LOCUS       DATITW010000001         1524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_480738, whole genome shotgun sequence.
ACCESSION   DATITW010000001 DATITW010000000
VERSION     DATITW010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480738"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1524)
                     /gene="fusA"
                     /locus_tag="VLS86_00005"
     CDS             complement(<1..>1524)
                     /gene="fusA"
                     /locus_tag="VLS86_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020572746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSO48899.1"
                     /translation="SAATTCVWHEHWINIIDTPGHVDFTVEVERSLRVLDGAVAVFDG
                     VAGVEPQSETVWRQADRYHVPRICFINKLDRTGADFYADVDSITDRLGAHPLVMQLPV
                     GTEGDFMGVVDLVEEKAHLWRGDDGKQWDTTEIPEDLVVLSAKYREALIEKVAETSED
                     LFEAYLEHGTLTIDQIKAGVRIGTLQGDFNPVFCGSAFKNKGVQLLLDAVVDYLPSPA
                     DLPPIKGFKPGDEEVVIERKLEDTEPFSALAFKIMSDPHVGKLTYFRVYSGHAAKGDS
                     VLNTTTGKKERFGRILRMHANSREDVDEVFAGDIVAGIGFKSTTTGDTLSSERAPIQL
                     ESMEFPAPVISVAIEPKTKSDDEKLSESLHRLTEEDPTFLVRTDEETGQTIISGMGEL
                     HLEILVDRLLREFKVDATVGKPQVAYRETIRRAIEKVVYRHVKQTGGKGQYAHVVINL
                     EPTSPGGGYEFVDKITGGRVPREFIPAVDQGIQSALDSGVLAGYPTVDVRAVLVDGQY
                     "
BASE COUNT          260 a          472 c          496 g          296 t
ORIGIN      
        1 gtactgcccg tcgactagca cggcccgcac gtccaccgtc ggatagccgg cgagcacacc
       61 cgagtcgagg gccgactgga tgccctggtc gacggccggg atgaactctc ggggcacgcg
      121 cccgccggtg atcttgtcga cgaactcgta accgccgccg gggctggtcg gctccaggtt
      181 gatgacgaca tgggcgtact gccccttgcc gccggtctgc ttgacgtgtc ggtaaaccac
      241 cttttcgatg gcccggcgga tggtctcccg ataggcgacc tggggcttgc cgacggtggc
      301 gtcgaccttg aactcacgca gcagacggtc cacgaggatc tccaggtgga gctcccccat
      361 tccggagatg atggtctggc ctgtctcttc atcggtccgg accaggaagg tggggtcttc
      421 ttcggtgagt cgatgcaggg actcggagag cttctcgtcg tcggacttgg tcttgggctc
      481 gatcgcaacc gagatcaccg gggccgggaa ctccatcgac tcgagctgga tcggtgcccg
      541 ttctgaagac agggtgtctc cggtcgtggt gctcttgaaa ccgatgcccg ccacgatgtc
      601 gccggcaaac acctcgtcga cgtcctctcg cgagttggcg tgcatccgga gaatgcgccc
      661 gaagcgctcc ttcttgccgg tcgtggtgtt caggaccgaa tcacctttgg ccgcatgccc
      721 ggaatacacc ctgaagtagg tgagcttgcc gacatggggg tcggacatga tcttgaaggc
      781 gagagccgag aagggctcgg tgtcctcgag tttgcgctcg atgacgacct cctcgtctcc
      841 aggtttgaag cccttgatgg gtgggaggtc ggccgggctg ggcagatagt cgacgaccgc
      901 atccaacagc agctggacac ctttgttctt gaatgccgaa ccgcagaaga ccgggttgaa
      961 atcgccttgg agggttccga tgcgcactcc ggccttgatc tggtcgatgg tcagcgtccc
     1021 gtgctcgaga taggcctcga acaagtcctc agaggtctcc gccaccttct cgatgagcgc
     1081 ctcgcggtac ttggcggaga ggacaaccag atcctcgggg atctccgtgg tgtcccactg
     1141 cttcccgtcg tcgcccctcc agagatgagc cttctcctcc acgaggtcga cgacacccat
     1201 gaagtcaccc tcggtgccca ccggcagctg catcacgagg ggatgagcgc cgagccggtc
     1261 tgtgatcgag tcgacgtcgg catagaagtc ggcgccggtg cggtcgagtt tgttgatgaa
     1321 acagatgcgg gggacgtggt agcgatcggc ctggcgccac accgtctccg actgcggctc
     1381 cacaccggcg accccgtcga atactgcaac tgcgccgtcc agaacccgga gcgaacgctc
     1441 cacctcgacg gtgaagtcga catggcccgg ggtgtcgatg atgttgatcc agtgctcgtg
     1501 ccacacgcag gtggtggcgg cgga
//
LOCUS       DATITW010000002         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_600987, whole genome shotgun sequence.
ACCESSION   DATITW010000002 DATITW010000000
VERSION     DATITW010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600987"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..609)
                     /locus_tag="VLS86_00010"
                     /pseudo
     CDS             complement(166..609)
                     /locus_tag="VLS86_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(609..1019)
                     /gene="ndhC"
                     /locus_tag="VLS86_00015"
     CDS             complement(609..1019)
                     /gene="ndhC"
                     /locus_tag="VLS86_00015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSO48900.1"
                     /translation="MTLADYLPIGLMLLLAAGFVAVSLVVSRLFSPNNPTEAKLDPYE
                     SGIVPETEPVQRFPVKFYLVAMLFVIFDIEIIFLFAWASLFDTLGWYGVAVVFIFTFF
                     VAETLAYVWKRGALDWNVHRRSRYITADASDEAA"
     gene            1111..1848
                     /locus_tag="VLS86_00020"
     CDS             1111..1848
                     /locus_tag="VLS86_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HSO48901.1"
                     /translation="MEAGSVGYGPECPHAVDRVAMLHRWDRLAFLHWRYDSGVVQQLL
                     PEGLVVEEYQGSAWVGLVPFFMEVRFPRVPRIPWLLEFPETNVRTYVTGPGGESGVWF
                     FSLDAARLAAVVAARDTYRLPYFWSQMRVSRRDDAITYQTRRRFGPRNASSSVVVEIG
                     SPYLPDDLEELDHFLTARWTLFGVQRKRLLMARAEHAPWALHRARALSWKDDLIEAAG
                     LPSPVGEPVVHWSPGVDVRVGFPHRVG"
     gene            1961..>3096
                     /locus_tag="VLS86_00025"
     CDS             1961..>3096
                     /locus_tag="VLS86_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSO48902.1"
                     /translation="MSSLRSALDEWIEEDVDQLHLDQLADDLVELEQVSGLIEAIRVQ
                     RIEAFERKAGHRSFGYPSMTACLKHRCRMASGRAWRLVAAARAIETAPHVFRAWLGNR
                     LSTDQTHRLLETAAAVPDEFPAAERLLIETVEDLSHTDTRRVLGYWRQSVDGPGTTTD
                     EVAQQEMRGISASVSLGGMLRIDGWMTPVAGQALLEAVQALMPPPDSGDTRTPRQRRH
                     DAIEDLARDFLDRGRTPTVGGEKPHVNVVCDLPALQGIAGGLHETETGHLLTISQLRT
                     ITCDCSLSRIVLGPDSEIIDVGRRTRGIPAALRRAIAARDRHCTWEGCDRNPHWCDVH
                     HIRHWADGGATEPTNLRLLCRFHHTLAHQADEPGRGPLVSGLSA"
BASE COUNT          558 a          974 c         1026 g          538 t
ORIGIN      
        1 ggacgcgggc cacatcctgg ccccgggaca gctcccagac cacgtccccg aactcctcga
       61 agagaccgcg gagcaggtcg ttctccggaa gcggaaccgg tggcgtctgc tcggtggtct
      121 cgaccgcgcc gttctcgtcg ctcacttgac gatctcgcct ttcatgatct tgtcgtgaag
      181 ggtgaggatc ccgtgcatca ggctttgcgg cccgggaggg cacccgggga cgtagatgtc
      241 gaccggcacc acctgatcga ccccttggac cagcgcatag ttgttgaaca tcccgccggt
      301 agaggcacag gcacccatcg agaccaccca tatcgggtct ggcatatgat cgaagacctg
      361 tcgcaggacc ggggccatct tctgactcac ccgaccggcg acgatcatga gatcggcctg
      421 gcgtggcgac gcccggaaca cctccatccc gaaccgggcc aggtcgtaat gggcggcccc
      481 ggtggccatc atctcgatgg cgcagcaggc aaggccaaag gtcgccggga acatggaacg
      541 ggtgcgggcc cagcgtgcgg ccttgtccac cgtagtcatg aggaggttgc ccggggcggt
      601 ggtggccact aagcagcctc gtccgacgcg tcggcggtga tgtacctgga gcgtcggtgc
      661 acgttccagt cgagtgcccc ccgcttccag acataggcga gggtctcggc gacgaagaag
      721 gtgaagatga agaccacggc cacgccgtac caccccaggg tgtcgaagag cgaggcccag
      781 gcgaagagaa agatgatctc gatgtcgaag atcacgaaca gcatcgccac cagatagaac
      841 ttgacgggga accgctgcac gggctccgtc tcggggacga tgccgctctc gtaggggtcg
      901 agcttggcct cggtggggtt gttggggctg aacaaccggg agacgacgag ggacaccgcc
      961 acgaatcctg cggcgagcag gagcatcagg ccgattggca gatagtcggc gagagtcacc
     1021 atcggagctt aggacgcggc acagggtggg ttaaatcagg tgggccgagg gctacggtga
     1081 ggcctacctc atctcggtta ggaaccatcg atggaggccg ggtcggttgg ctacgggcca
     1141 gagtgcccac atgcagtgga tcgggtggcg atgctccacc gatgggaccg attggccttt
     1201 ctccactggc gatacgactc cggcgtggta cagcagcttc tccccgaggg tctggtcgtc
     1261 gaggagtacc aggggtcggc gtgggtcggg ttggtgccgt tcttcatgga agtgcgcttt
     1321 ccccgggtcc ctcggattcc ctggctcctg gaattcccgg agaccaatgt tcggacctac
     1381 gtgacaggcc ccggcggcga gtcgggggtg tggttcttct cgctggatgc ggcccggctg
     1441 gccgcggtgg tggcggccag ggacacctac cggctcccat acttctggtc tcagatgcgc
     1501 gtcagcagac gtgacgatgc catcacctac cagacacgcc gacgattcgg gccgcgcaat
     1561 gcctcatcat cggtcgtggt cgagatcggt tctccctatc tccctgatga cctcgaggag
     1621 ttagatcatt tcctgaccgc gcgctggacc ttgttcgggg tgcaacgaaa acgtctgctg
     1681 atggcaaggg ccgagcatgc cccctgggcc ctgcatcggg cccgagccct gagttggaag
     1741 gacgacctca tcgaagctgc cggtctgcca tcgcctgtcg gtgagcccgt ggtgcattgg
     1801 tcacccggag tcgacgtgcg ggtcggtttt ccgcaccggg tggggtgagg cctcgcggcg
     1861 gatggtcgag aaaaacctca aacgaccccc tggaatctgt cagtgggggt cggtaggtta
     1921 gcgaacatat gttcgagaga gacatattgg ggagagaatc atgagctcct tgcgatcggc
     1981 actcgacgag tggatcgagg aagacgtcga ccagcttcac ctcgaccagc tagccgatga
     2041 tctcgtcgaa ctggagcagg tgagtggcct gatcgaagcg atccgggtgc agcggatcga
     2101 ggcgttcgag cgcaaggccg gccatcggtc attcggctat ccctcgatga ccgcctgtct
     2161 caagcaccgt tgtcgcatgg ccagcggccg ggcctggcgg ctggtggccg cagctcgggc
     2221 catcgagacc gcaccccacg tcttccgggc gtggctcggc aacagactct ccaccgatca
     2281 gacccaccga ctcctggaga ctgccgccgc agtcccggac gagttccccg ccgctgaacg
     2341 tctcctgatc gaaacggtgg aggatctctc ccacaccgac acccgccgcg tgctcggcta
     2401 ttggcgtcag tcggtcgatg gccccggcac cactaccgac gaagtcgcgc agcaagagat
     2461 gaggggcatc tcggcttcgg tcagtctcgg cggcatgctg cggatcgacg ggtggatgac
     2521 ccccgttgcc ggtcaagcct tgctcgaggc tgtgcaggcg ctgatgcccc caccagattc
     2581 gggcgacacc cgcactccgc gccaacgtcg tcacgacgca atcgaagacc tggcgcgcga
     2641 tttcctcgac cgcggccgga cgccgacggt tggaggcgag aagccccacg tcaacgtcgt
     2701 ctgtgatctt cccgctctcc aggggatcgc gggcggtctc cacgagaccg agaccggaca
     2761 tctgctcacg atcagccagc ttcgcaccat cacctgcgac tgctcgctga gcaggatcgt
     2821 gctcgggccc gactcggaga tcatcgacgt cggaagacgc accaggggga tcccggctgc
     2881 cttgagacga gcgatagccg cgagagatcg gcactgcacc tgggagggat gtgaccgcaa
     2941 cccgcattgg tgtgacgtcc accacatccg acactgggcc gacggcggcg ccaccgagcc
     3001 gaccaatctg aggcttctgt gtcgcttcca ccacacgctg gctcaccagg ccgacgagcc
     3061 gggtcggggt ccactggtca gcgggttgtc ggcttt
//
LOCUS       DATITW010000003         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_280596, whole genome shotgun sequence.
ACCESSION   DATITW010000003 DATITW010000000
VERSION     DATITW010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280596"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VLS86_00030"
     CDS             complement(<1..880)
                     /locus_tag="VLS86_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSO48903.1"
                     /translation="MAAENPEVSKQGSSGAPGTPERESLLDQLRAANEQLVVTSMRAQ
                     DLADEADASRAAAEAANRLKDEFLAVVSHELRTPLNAILGWAHLLGGEQLDRSRTLNA
                     IHIIERNAKALTRIIDDLLDISRIIGGTMRIDPLAVDLVAVIQGALDEVEPLAEAKDV
                     KVTFTCQVVPGPVGGDALRLQQIVANLLSNAVKFTPSGGKVQVRLTSTDILAEIQVAD
                     TGQGIAEQFLPTIFERFTQADPSTTRRQGGIGLGLAIVKALVERHGGTVHAESPGVGK
                     GATFTVRIPVLDPQEVD"
     gene            complement(890..2431)
                     /locus_tag="VLS86_00035"
     CDS             complement(890..2431)
                     /locus_tag="VLS86_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HSO48904.1"
                     /translation="MTESEKGVSIRKLPTGVPGFDDVLGGGLPELSFNLIVGGPGSGK
                     TTLAHQILFANATADRPGLYFTIVGEPPLKMLRYQQQYSFFDPAKVNGLIRFASLSGE
                     LEQGLTAVLDGITRQIEAAKPAFVVVDSFRSVMRVSSGADTHALELQDFIQRLALHLT
                     AWEATTFLIGEFTEPEAQDHPVFTVADSLIALSQKTERNSVVRKMRVMKMRGQGDLPG
                     LHTFRITSDGLKVFPRIPKPEEEPNQQDGSQARTSVPERLSTGVKGLDEMLNGGVPRG
                     YSVMLAGPSGSGKTVLAGQFIADGVKHGEPGVIAIFERRPAGYLQTTSLGRDLERMIH
                     EGKVAIVYLRPLDLSVDETLYELREAILRMKATRVVIDSLSGFEMAVSPGFREDFRES
                     LYRLVGVLTGLGVTVMMTLEIEDAYTELRLGPRQNAFLADAILLMRYVELGGTLRRVM
                     TVIKVRGSEHSKDLRLYEVHAQGLTIGESVTLYDHGLLTANPTRTSGPDRSPGRSRRG
                     RRRDS"
BASE COUNT          462 a          800 c          789 g          452 t
ORIGIN      
        1 cgtccacttc ctgaggatcg agaaccggaa tgcgcaccgt aaacgtcgcc ccctttccga
       61 cgccagggct ttcggcgtga acggtgccgc catgccgttc aaccagtgcc ttcacgatgg
      121 cgagtcccag gccaatccca ccctgtcgcc gcgtcgtcga gggatcggcc tgagtaaatc
      181 gctcgaagat ggtcgggaga aactgctccg cgatgccctg gcctgtgtcg gccacctgaa
      241 tctctgccag gatatccgtc gaggtgagtc gcacctggac cttcccccca gacggcgtga
      301 acttgacggc gttcgagagc agattcgcca caatctgctg caagcggagc gcatcgcccc
      361 cgaccgggcc gggcacaact tgacaggtga acgtcacctt cacatctttc gcttcggcga
      421 gcggctcaac ctcatcgagc gccccttgga tcacggcgac aagatccacg gccagcggat
      481 cgatgcgcat cgtgccgccg atgatccgcg agatgtcgag gaggtcgtcg atgatccgcg
      541 tcagcgcctt cgcattgcgc tcgatgatat ggatcgcgtt gagcgttcgc gagcgatcga
      601 gctgctcgcc gccgagcagg tgcgcccagc cgaggattgc attgaggggc gtccgcaact
      661 cgtgggacac gaccgccagg aactcatcct tcagccgatt cgcggcttcg gccgcagccc
      721 gcgaggcgtc cgcttcgtcc gcaaggtcct gggcccgcat tgaggtaacg accagttgct
      781 cattcgcggc gcggagttga tccagtaagg actcgcgctc aggcgtaccg ggcgcgccgg
      841 atgatccctg tttggacacc tccggattct cggcagccat ttggccctct tacgagtccc
      901 gacggcgacc acgcctggac cgtcccggcg atcggtcggg gccagacgtg cgcgtcggat
      961 tggcagtcag gagcccatga tcatagaggg taacgctttc gccaatggtc agtccttggg
     1021 cgtgcacctc gtagagccgc aagtccttac tgtgttcgct accccggacc ttgatgacgg
     1081 tcatcactcg cctgagtgtt ccgccgagct cgacgtatcg catgaggagg atcgcgtccg
     1141 cgagaaaggc gttctgacga gggccgagcc gaagctcagt gtaggcgtct tcaatctcca
     1201 gggtcatcat cacggtgacg ccgaggccag tcaggacacc gaccagccgg tagagcgact
     1261 cccgaaagtc ctcgcgaaac cccggcgaga ccgccatctc gaagccggag agcgaatcga
     1321 tgaccacacg ggtggccttc atgcgcagga tcgcctcgcg caactcgtac aatgtctcat
     1381 cgacggacag gtccaggggg cgaagataga caatggcgac cttgccctcg tggatcatcc
     1441 gttcgagatc gcggcccagc gaggtggtct ggagataccc ggctggacgc cgctcgaaga
     1501 tcgcgatgac gccgggctca ccgtgcttca ccccatcggc gatgaactgc ccggccaaca
     1561 ccgtcttgcc tgagccagag gggcccgcga gcatcacgga gtagccccgc ggcacgccac
     1621 cattcagcat ctcatcgagg cccttcaccc cggtcgagag gcgctccggg accgacgtgc
     1681 gcgcctggga cccgtcttgt tgattcggct cttcctccgg cttggggatg cgagggaaga
     1741 ccttgaggcc atcgctcgtg atgcggaagg tgtggagccc cggcagatca ccctgcccgc
     1801 gcatcttcat gacccgcatc ttgcgcacga cagagttccg ctcggtcttc tggctgagcg
     1861 caatgaggct gtcagccacg gtgaagacgg gatggtcctg agcctccggc tctgtgaact
     1921 cgccgatcag gaaggtcgtg gcctcccagg cggtgaggtg cagcgcgagc cgctgaatga
     1981 agtcctgcaa ctccagcgcg tgtgtgtccg ctccgctgct cacccgcatc acggagcgaa
     2041 aggagtccac gacgacaaag gccggtttgg cggcttcgat ctgacgggtg atcccgtcga
     2101 gcaccgccgt gaggccttgc tcaagctcgc cgctcagtga agcaaagcgg atgaggccat
     2161 tcaccttcgc cgggtcgaag aagctgtact gttgctggta gcggagcatc ttgagcggcg
     2221 gctcgccgac aatcgtgaag tagaggcccg gacggtcagc ggtggcattg gcgaacagaa
     2281 tctggtgggc caaggtcgtc ttgccagagc cggggccgcc gacgatcagg ttgaaggaca
     2341 actcaggaag tccgccaccc aggacgtcat cgaatccagg gacccctgtg gggagcttcc
     2401 gaatcgacac ccctttctcg ctctcggtca ttcgtgggac tcctggagtt caggttcggg
     2461 gaggaggatg tcaggccaaa tctgctgcaa caggctccat gtg
//
LOCUS       DATITW010000004         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_240569, whole genome shotgun sequence.
ACCESSION   DATITW010000004 DATITW010000000
VERSION     DATITW010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240569"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VLS86_00040"
     CDS             complement(<1..739)
                     /locus_tag="VLS86_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HSO48905.1"
                     /translation="MSQIVEGEIVKSKVLRVTDNAVILDVGFKSEGSVPLDEFKDPHS
                     LKEGDEVEVFLEHLEDQEGAVVLSKKKADFMRVWEKIRVAHESDQPVEGTLVKKIKGG
                     VVVNLMGVDAFLPGSQIALRRVPNIDELLGQTYEFKIIKLNKRRRNIVVSRRVILENE
                     RAHKREHLMKELAVGQVRKGVVKNITDFGAFIDLGGVDGLLHITDMSWGRINHPSEMV
                     KLDQEIEVVVLRVDQDRERIALGLKQKT"
     gene            complement(1049..>1527)
                     /locus_tag="VLS86_00045"
     CDS             complement(1049..>1527)
                     /locus_tag="VLS86_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HSO48906.1"
                     /translation="LQPDGAGFAAYFEGEPVDVEIRSAEVTGQVSAISAIPVVREWIN
                     SRLRALVRAGQDVVVDGRDIGTVVVPEADLKNFLTASPEARAGRRISQRGMPVQPDGL
                     DAEAAALAARDHADSTRAVAPLRPAGDAVHLDTTTMDFAEQVRFIVERARPIFSRR"
BASE COUNT          264 a          493 c          476 g          294 t
ORIGIN      
        1 gcgtcttctg cttcaggccg agcgcgatgc gctcgcgatc ctgatcgacg cgcaagacga
       61 ccacttcgat ctcttgatcg agcttgacca tctcgctcgg gtggttgatg cggccccacg
      121 acatgtcggt gatgtggagc agaccgtcca caccgccgag atcgatgaac gcgccgaagt
      181 cggtgatgtt cttgaccacg cccttccgca cctggccgac ggcgagctcc ttcatcaggt
      241 gctcgcgctt gtgggcccgc tcgttctcca gtatcacccg gcggctgacc acgatgttcc
      301 ggcgccgctt gttgagcttg atgatcttga actcgtaggt ctgacccagc agctcgtcga
      361 tgttgggcac ccgccggagc gcgatctggc tgccgggcag gaaggcatcg acacccatca
      421 ggttcaccac cacaccaccc ttgatcttct tgaccagggt accttcgacc ggttgatcgc
      481 tctcgtgcgc cacgcggatc ttttcccaga cccgcatgaa gtcggccttc ttcttcgaca
      541 ggacgacggc gccttcctgg tcctccagat gctcgaggaa gacttcgacc tcatcgccct
      601 ctttcagcga atggggatcc ttgaactcat ccagcggaac cgagccctcg gacttgaacc
      661 cgacgtcgag gatgaccgcg ttatcggtga ctcggaggac cttcgatttg acgatctcgc
      721 cttcgacgat ctgcgacatg gtgccctcat acatcgagag catctgttcg tagtcggcgt
      781 cgctatagtc ttcgtcggag aggtcctgcc gaaagcggag gccggtcggg gagatgaact
      841 ggtcggtcga gtggtcagtt gagggattga gttggtcgag catgcgaagt tctgccgtcc
      901 tttacgtgcc gcgccggcta gcgccgcggg ccgaagttga gccgcgccag aggcggaagt
      961 gccgctggcg agagcccatg aggatagcgg ccgaagcccc acaacgcaag tcctgtcaat
     1021 cggttgggac ttgagatatg gcgtcgtctc agcggcggga gaagatcggc cgggcccgct
     1081 ccacgatgaa gcggacctgt tcggcaaagt ccatcgtggt ggtgtcgagg tgcaccgcgt
     1141 cgccggcggg tcgaagcggt gccacggccc gggtagagtc cgcatggtcc cgcgccgcca
     1201 gggccgccgc ctcggcatcg aggccgtcgg gctggaccgg catgccccgc tggctgatgc
     1261 ggcggccggc tcgagcctcc ggcgaggcag tcaggaagtt tttgagatcg gcctcgggga
     1321 ccacgactgt accgatgtcc cgcccatcca ccacgacatc ctggcccgca cgaactaggg
     1381 cccgcagtct cgaattgatc cattcccgga ccaccggaat ggcagagatg gcggagacct
     1441 gcccagtgac ctccgcgcta cggatctcca catccaccgg ttccccttcg aaataggcgg
     1501 caaacccggc tccatccggc tggagca
//
LOCUS       DATITW010000005         1723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_440942, whole genome shotgun sequence.
ACCESSION   DATITW010000005 DATITW010000000
VERSION     DATITW010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440942"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..519)
                     /gene="rimI"
                     /locus_tag="VLS86_00050"
     CDS             complement(43..519)
                     /gene="rimI"
                     /locus_tag="VLS86_00050"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HSO48907.1"
                     /translation="MTAVTIRAFDDADVDAAVALEANDRLKPWSAGVFRDELAAENRV
                     YLVADSDALVGFGGVMIVGDEAHVTNLLVDSESRRQGIGSLLMLALIVASIERGARHL
                     TLEVRSRNHAARSLYAAFGLAPVGLRKGYYGDDDALVLWAHEIDSDEYRQRLESLR"
     gene            complement(516..1220)
                     /gene="tsaB"
                     /locus_tag="VLS86_00055"
     CDS             complement(516..1220)
                     /gene="tsaB"
                     /locus_tag="VLS86_00055"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HSO48908.1"
                     /translation="MLAIETSTPGSSVALVEDASTLATASRIDRMGHATFLVPAIDFC
                     FDQVGWSPSALDAVVVDIGPGLYTGIRVGLATAQGLAAAFGIPVIPAMSLDAIALEAR
                     TGHRLIWALVDVRRGELAVARYRPVPGGVVKEGAVELTRPEVLAAQLDSSPDESLVVG
                     DVSALPDGLLLGLHRVKTGRPRYPYAVTLAEAARAKFERGEFPAPDEIRPLYLREPDV
                     TINWAKLRQEGPWGSE"
     gene            complement(1223..>1723)
                     /gene="tsaE"
                     /locus_tag="VLS86_00060"
     CDS             complement(1223..>1723)
                     /gene="tsaE"
                     /locus_tag="VLS86_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HSO48909.1"
                     /translation="GDRGDRRRSSPDGVVRMIEVLCPTPDDTRAAGRRLAAALRPGDV
                     VELAGGLGAGKTLFTGGLCEGLGVEEMVVSPSFVLVRQYGSGFMPVVHVDVYRLGSLH
                     EFEDLDVFELAADGVLVIEWGDAVESALPEDHLRIEFVVSQDESRLLRLIPSGDWVER
                     TLETVT"
BASE COUNT          349 a          604 c          504 g          266 t
ORIGIN      
        1 cggtctcgtc acagctggtc tcgaaggcga ggactagtgg cgtcatcgaa gactctccag
       61 ccgctggcgg tactcatcgc tgtcgatctc gtgcgcccag agcaccaggg cgtcgtcgtc
      121 cccgtagtag cccttcctca acccgaccgg agccagaccg aaagcggcat acagagatcg
      181 cgcagcgtgg ttccgggagc gcacttcgag tgtgagatga cgcgcaccgc gctcgatcga
      241 ggcgacgatc aaggcgagca tcagaaggct tccgatccct tgccgccgcg actccgaatc
      301 gacgagcagg ttggtgacat gagcctcgtc accaacgatc atcactcctc caaacccaac
      361 cagggcgtcg gagtcggcga ccagataaac ccggttctcg gccgccaact cgtcacgaaa
      421 gaccccggcg ctccagggtt tcaaccgatc gttcgcctcc agggccacgg cggcatcgac
      481 gtcggcatcg tcgaaagctc tgatggtcac ggcggtcact ccgaacccca tggcccctcc
      541 tgacgcaact ttgcccaatt gatcgtcacg tcgggctctc gcaggtagag gggacggatc
      601 tcatccggag caggaaattc gccgcgttcg aacttggccc gggcggcctc ggccagggtc
      661 accgcatacg gatagcgggg gcgaccggtc ttcacccgat gcaggccgag caagagcccg
      721 tcggggaggg cgctgacatc accaaccacc agactctcgt ccgggctcga gtcgagttgg
      781 gccgccaaca cctcgggtct cgtcaactcc actgcacctt ccttgaccac tcccccgggg
      841 accggccgat agcgagcaac ggccaactcg ccccgacgca catcgaccaa agcccaaatg
      901 agacgatgcc cggtacgggc ctcgagggct atcgcatcca gtgacatcgc gggtattacc
      961 gggatcccga acgcagccgc gagtccctgc gccgtggcca agccaacgcg aatcccggtg
     1021 tataggccag gcccgatgtc gacgaccacc gcgtcgagcg ccgacggaga ccacccgacc
     1081 tggtcgaagc agaagtcgat ggccgggacc aggaaggtgg catgacccat ccgatcgatc
     1141 ctcgaagccg tggccagggt cgaggcgtcc tcgaccaggg caactgacga tccaggtgtc
     1201 gaggtttcga tagcgagcaa tctcacgtga ctgtctccaa cgtgcgctcg acccagtccc
     1261 cgctcgggat gagacgcaac agcctcgact cgtcctggct cacgacgaac tcgatccgga
     1321 ggtggtcttc gggcagggcc gattcgacgg cgtctcccca ttcgatgacg aggacgccat
     1381 cggcagccaa ctcgaacacg tcgagatcct cgaattcgtg gagcgatccg agtcgataca
     1441 catcgacgtg aaccaccggc atgaacccag aaccgtattg acgcaccaat acgaacgacg
     1501 ggctgaccac catctcttcg acacccagcc cctcgcacag cccgcccgtg aacagggtct
     1561 tgcccgcccc cagacctccg gcgagctcca ccacatcgcc cggtctcagc gcggcagcga
     1621 ggcgccgacc tgcagcccga gtgtcgtcag gcgtggggca caacacctcg atcatccgaa
     1681 caactccatc tgggctggat cggcgacggt ctcctcggtc tcc
//
LOCUS       DATITW010000006         1909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_761425, whole genome shotgun sequence.
ACCESSION   DATITW010000006 DATITW010000000
VERSION     DATITW010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761425"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VLS86_00065"
     CDS             complement(<1..446)
                     /locus_tag="VLS86_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HSO48910.1"
                     /translation="MNKQAEYVLRTVEERGIRFVRLWFTDVQGFLKSVSISPAELEVA
                     FEEGMTFDGSAIDGYARIQEADMLARPDPSTFAVLPWRTEQQVARMFCDIATPDGEPF
                     PGDPRIVLKRNLDRASEMGYTFYAAPELEFFYFRDSGPEPEVLDRGG"
     gene            complement(477..1613)
                     /locus_tag="VLS86_00070"
     CDS             complement(477..1613)
                     /locus_tag="VLS86_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO48911.1"
                     /translation="MAMVFFVNGVVVGSWLPRLPEIRDRLGIDLGALGLTLALGGVGS
                     LVGSSLSGVTVGRFGARRSAVAAGVVLYLILPFIAVAPTATVLAVVLATMGFIDAQAD
                     VGMNAVGIRVEEAVGRSIMTRLHGLWSLGTLVGAGASALFVLAGIDLLLQLVILAVLG
                     LITTLVAARLVPESPPRAAVGQRSGTLAIGLMLAGGTAVFIEGSPFEWSAIFLSDVVG
                     ATAALAGAGVILFTGGMLTGRLAGDHLVDRFGAVPTLFAGFVISVAAMLGTVTTSVVG
                     VALVGFAFWGLGISVVLPVLYKLAGGHESFAEGSGLAALTVGTRLGVMVSPALIGVAA
                     TAWGLPTALAVVVGVAAVASMLVIRLTLGSGGSVRDSLQETGVS"
BASE COUNT          359 a          654 c          621 g          275 t
ORIGIN      
        1 cccccgcggt cgagtacctc gggctcgggc cccgagtcgc gaaagtagaa gaactcgagc
       61 tcgggagccg cgtagaaggt gtagcccatc tccgaggcgc ggtccaggtt ccgcttgagc
      121 acgatccggg ggtctccggg gaagggctca ccgtcgggcg tcgcgatgtc gcagaacatc
      181 cgagccacct gctgctcggt ccgccaggga agcaccgcga acgtcgacgg gtcgggtctg
      241 gccagcatgt cggcttcctg aatgcgggca tagccgtcga tggctgatcc gtcgaaggtc
      301 attccttcct cgaaggcgac ttcgagctcg gcagggctta tcgagaccga cttgagaaac
      361 ccctggacgt cggtgaacca gagccggacg aagcggatgc cacgctcctc aacggttcgt
      421 agtacgtact cggcttgctt gttcatctct gattccctgt ctccggtcgt cacacattat
      481 gagacacctg tctcctgaag ggaatcgcga accgaccctc cgcttccaag ggtgagacgg
      541 atgacgagca tcgacgccac ggcagccacc ccgaccacca ccgccagagc agtgggcagg
      601 ccccatgccg tggcggcgac accgatcagt gctggtgaca ccatgacccc gagacgggtc
      661 ccgaccgtga gcgcagccag cccggaccct tcggcaaacg actcgtggcc gcccgccagc
      721 ttgtagagga ccgggaggac gaccgagatc ccgagacccc agaatgcgaa gccgacgagg
      781 gcgactccca cgacagaggt ggtcaccgtc cccaacatcg cagccactga gatgacgaag
      841 ccggcgaaca acgtcgggac cgccccgaat ctgtccacca ggtggtctcc ggccagacgt
      901 ccggtcaaca tccctccggt gaacaggatc actccggctc cggcgagggc agctgtggcg
      961 cccaccacgt cgctgaggaa gatggcgctc cactcgaatg gagagccttc gatgaacacc
     1021 gcggtcccac cggcgagcat caacccgatg gcgagagtcc ccgatcgctg cccgaccgcg
     1081 gcgcggggag gggactcggg tacgagtcgg gcggccacca aggtcgtgat cagccccagg
     1141 acggcgagga tgaccagttg cagcaagaga tcgatcccgg ccaggacgaa gagcgcactg
     1201 gcaccggcgc cgaccagggt gccgaggctc cacagcccat ggagccgggt cattatcgac
     1261 cgtcctacgg cctcttcgac cctgatcccg accgcgttca tcccgacgtc ggcttgggcg
     1321 tcgatgaatc ccatggtggc cagcaccacc gccagcactg tcgccgttgg tgccacggcg
     1381 atgaagggga gaatcaggta gaggacgaca ccggccgcca cggccgaccg gcgggccccg
     1441 aaccgcccca cggtgactcc gctcagcgag gagccgacca gtgagcccac tccgccgagg
     1501 gcgagagtga ggccgagagc cccgaggtcg atgcccaatc ggtccctgat ctcggggagc
     1561 cggggaagcc agctcccaac cacgaccccg ttgacgaaga acaccatcgc caccccgaga
     1621 gtggcccgtt tgacgatggt cagctcacgg cctcgcgccg acccggctcc atcccgcgac
     1681 ttaccaggca gacaccgcga tgtcgccggt cagcggcctg ccccggggtg gatgggaagg
     1741 agttggtggt cacccttcgg agtttggcca gccggtccgc gttttaccag gaccagcgca
     1801 gccaagctcg gtccggtcac atgtcgtcac gcgggttcgg gcctgggtcg tcgtcgggca
     1861 catcgtcagg gcgctcgtcg ggcacatcgt cagggcgctc gtcgggccg
//
LOCUS       DATITW010000007         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_481117, whole genome shotgun sequence.
ACCESSION   DATITW010000007 DATITW010000000
VERSION     DATITW010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481117"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /gene="msrB"
                     /locus_tag="VLS86_00075"
     CDS             <1..249
                     /gene="msrB"
                     /locus_tag="VLS86_00075"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006618845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HSO48912.1"
                     /translation="GSDLFGSDTKFDSGTGWPSFWAPIAKESIRAEMDNSLSMRRVEV
                     MCSRCDAHLGHVFEDGPKPTGLRYCINSASLNFLEKGE"
     gene            complement(379..1185)
                     /locus_tag="VLS86_00080"
     CDS             complement(379..1185)
                     /locus_tag="VLS86_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSO48913.1"
                     /translation="MAYTTLKLEFEGLSLSGVVGELATITLNRPEKRNAISTEMIEEL
                     LAALDEVEASAARVVVVTGAGKAFCSGMDLDALKAVSSQSPEQYLEDCRRVARLFRRL
                     YSFPKPLIAAVNGPAIAGGCGIATLCDFTLAAPEARFGYSEVRIGFIPAIVSVFLIRQ
                     IGEKRARDLLLTGRILGAAEAHQIGLVTEVVPAGILFDRARELAATLIAASPTSLLLT
                     KRLLGHLWEADLDREIELAIRENARSRSTADFREGVASFLEKRNPVWHGD"
     gene            complement(1229..>2082)
                     /locus_tag="VLS86_00085"
     CDS             complement(1229..>2082)
                     /locus_tag="VLS86_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HSO48914.1"
                     /translation="PRDAWQGLPQVVPTEVKVEYLRRLVELGFRHIDAVSFVSPKHVP
                     QMADSEAVMKALCASLPRGADPPEIIGIVMNQKGLQRALAAPGVTTIGYPHSISATFL
                     RANSNLSLSESRGMIEKLAKETGAAGRSLLVYISMAFGNPYQEAWEPDIVQETLEWLA
                     GIDVRTVSLADTVGHATPEQVGSLFGAVKDSAGEMELGVHLHSRHEDAAEKVLAAHEA
                     GCRRFDSVLTGLGGCPFAGDQLVGNIPTEIVVPTLARRGAALDVSVASLDHATTMAQE
                     MCSKYAR"
BASE COUNT          371 a          667 c          667 g          377 t
ORIGIN      
        1 ggcagcgacc tgttcggttc agacaccaag ttcgactccg gcaccgggtg gccaagcttc
       61 tgggccccga tcgccaagga gagcattagg gccgaaatgg acaacagcct ctccatgcgg
      121 agagtggagg tgatgtgcag caggtgcgat gcccacctcg gccacgtctt tgaggacggg
      181 ccaaagccca cgggactgcg ctattgcata aattccgcat ctcttaactt tcttgagaaa
      241 ggtgagtagg gcagagggtt tcttgccttt ctcctcccct cggagacaag gagtatcttg
      301 ctcccctctc cccgctgggg agagggttcg ggtgaggggg accctcggcg caatgagact
      361 tcacctagcg cgtctgtatt agtcgccgtg ccagacaggg ttgcgcttct cgaggaagga
      421 ggcaacccct tcgcgaaagt ccgccgtcga gcggctgcgg gcattctcgc ggatggccag
      481 ttcgatctcg cggtccaggt cggcctccca gaggtgtccc agcagtcgct tggtgaggag
      541 gaggcttgtg gggcttgcgg caatcagcgt ggcggcgagc tcccgggccc gatcgaaaag
      601 tatcccagcc ggcacgacct cggtcaccag gccgatctgg tgcgcctctg cggccccgag
      661 gatgcgccca gtcagcagaa ggtcgcgtgc ccgtttctcg ccgatctgac gaatcaggaa
      721 caccgagacg atggccggga tgaagccgat gcgaacctcg ctgtagccga acctggcctc
      781 gggcgcggcg agggtgaagt cgcagagcgt cgcgatcccg cagccgccgg cgatggccgg
      841 gccgttgacc gcggcgatca gcggcttcgg gaagctgtac agacggcgaa agagacgcgc
      901 cacccgccga cagtcttcca ggtattgctc gggcgattgt gacgaaacgg ctttcagcgc
      961 gtcaaggtcc atcccggagc agaacgcctt gcctgcgccc gtcacgacga cgactcgcgc
     1021 ggcactggcc tccacctcgt ccagcgccgc aagtagctct tcgatcatct cggtggagat
     1081 cgcgttgcgc ttctccggcc gattgagggt gatagtggcc agctccccca cgaccccgct
     1141 cagggaaaga ccttcgaatt cgagcttcag tgtggtatag gccattgagc ttctctctga
     1201 ttcatcttcc gacccggaca gggaagcgct agcgggcgta cttgctgcac atctcctggg
     1261 ccatcgtcgt cgcgtgatcc agcgatgcca cggagacatc gagggcggcg ccgcgccgag
     1321 ccagcgtggg cacgacgatc tcggttggga tattgccgac aagttgatcc ccggcgaacg
     1381 ggcacccgcc caatccggtg agcacgctgt cgaatcgccg gcacccggct tcgtgggcag
     1441 cgaggacttt ctcagcggca tcctcgtgcc ggctgtgcag atggaccccc agttccattt
     1501 cgcccgcgga atccttcact gctccgaaaa gactgcccac ctgttcgggt gtggcgtgac
     1561 ccaccgtgtc cgcaagcgag accgtgcgca cgtctatgcc cgccaaccac tcgagtgtct
     1621 cttggacgat gtccggctcc cacgcctcct gataggggtt cccaaaggcc atcgagatat
     1681 agaccaacag gctgcgaccc gcggcgcccg tctccttcgc gagcttctcg atcattccgc
     1741 gggattccga gagggagagg ttggagttgg cccgtaggaa ggtcgccgag atggagtgcg
     1801 ggtagccgat cgtcgtcacg cccggtgcgg ccaaggcgcg ctggagacct ttctggttca
     1861 tcacaatgcc gatgatctcg ggagggtcag cgccccgggg gagcgacgcg cacagcgcct
     1921 tcatcaccgc ctcgctatcg gccatctggg ggacgtgctt cggcgagacg aaactcacgg
     1981 cgtcgatatg ccggaacccc agctcgacga ggcgtcgcag gtattcgacc ttgacctcgg
     2041 tcggaaccac ctgcggcaaa ccctgccagg catccctcgg gc
//
LOCUS       DATITW010000008         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_360949, whole genome shotgun sequence.
ACCESSION   DATITW010000008 DATITW010000000
VERSION     DATITW010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360949"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VLS86_00090"
     CDS             <1..1341
                     /locus_tag="VLS86_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO48915.1"
                     /translation="DLLTAPPPDLPSVVARGRRKQMTKKLASGVAAAALIVGAVMLIQ
                     AQPFEPPLVSQPTSTTTVSSQSAVNGWVAFAANNSAGDNDIYVARVGESPRRIMGFDS
                     DGLEQVCPAFSPDGARLAYGQAQGRSEVGYSGGLLVVASLDREGNASTSLELDVGATS
                     APPCPLWSPDGERIAITVHSGDRVSPPQGSDVEGDVWIVNLDSGKTTVLSGLYVWDDG
                     WGFPDMEWSPDGAELAIANGQVTIYSAVTGESRAMDGATGAEALTWSEDGTRIAFQRF
                     GLGPHSSSSPWRGGELRIAEVDGTGGYLLADGFMAFHGIGPVWSPLGDRIVYQRVCST
                     YASSFSPTQPCREEHDVVLVTLDGTETVLPHLRLPGSDATEPMWPYRVTWSPDGEQLL
                     YQVWGSADSDFTEALIAVPLDPSSPPIVLYEGDGGYVDDGWFAFQSWGRQPGAP"
     gene            1605..2075
                     /locus_tag="VLS86_00095"
     CDS             1605..2075
                     /locus_tag="VLS86_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HSO48916.1"
                     /translation="MALVVGLIVMAIAGAVLATPGSGFTAAQQWKGVFGDIDVHLVDM
                     PGHQVKVKTNGVSDVYVTRNAIAVDGHSGWHTHPGPSLIMVTVGEIMAYDGDDPTCTP
                     TLYTTGEGFVDEGGGHVHLLRNESGAPAETVAIQFLPKDATRRIDMPDPGNCSF"
BASE COUNT          407 a          679 c          700 g          404 t
ORIGIN      
        1 gatctcttga ccgcgccgcc gcccgacctc ccgagtgtcg ttgcgcgggg ccgacgcaag
       61 cagatgacaa aaaagctcgc ctcgggtgtg gctgccgcgg cgttgatagt cggcgcggtc
      121 atgttgatcc aggcccagcc gttcgagccg ccgctggtca gccaacctac atccacaacc
      181 actgtgtcct cccagagtgc ggtgaatgga tgggtggcct tcgccgccaa caactcggct
      241 ggcgataacg acatctacgt ggcgcgcgta ggtgagtctc cccgccggat catgggcttc
      301 gattcggatg gtctggaaca ggtgtgtccg gccttctcgc cggatggtgc tcgactcgcc
      361 tacggtcaag cacaagggag atcggaggtc ggttacagcg gcggtctgtt ggtcgtcgcg
      421 agtcttgatc gggagggcaa tgcctcgacg tcgctcgagc tcgacgtcgg cgcgacctca
      481 gccccgccgt gtccactgtg gtctcccgat ggtgagagga tcgccatcac cgtccattca
      541 ggtgatcgag tgagccctcc tcagggatcc gatgtcgaag gcgacgtctg gattgtcaac
      601 cttgacagcg ggaagacaac agtgctgtcg ggtttgtacg tctgggacga tggctggggg
      661 ttccccgaca tggagtggtc tcccgacggc gccgagcttg ccatcgccaa cggtcaggtc
      721 accatctatt cggccgtcac tggcgagtcg cgagccatgg acggcgcgac aggggcggaa
      781 gccttgacct ggtccgagga cggcacccgg atcgcctttc agcgtttcgg cttgggaccg
      841 cattcctcga gctctccatg gcgaggtggc gaactccgga tagccgaagt cgatggcacc
      901 ggaggatatc tgctggctga cggtttcatg gccttccatg gaattggacc ggtgtggtct
      961 cccctcggcg atcgcatcgt ctatcagcga gtctgcagca catacgcttc gagcttctcg
     1021 ccgacgcagc catgccggga agagcatgac gtggtcctgg tcactcttga tggaaccgag
     1081 accgtgcttc cccatctccg actgccgggc agcgatgcga ccgagccgat gtggccatac
     1141 cgcgtcacct ggtcacccga cggcgagcaa ctcctctatc aggtatgggg atcagcagat
     1201 tcagatttca ccgaggccct cattgcggtt ccgctcgacc cgagttcacc gccgattgtg
     1261 ctctacgagg gggatggggg gtacgtcgat gacggttggt tcgccttcca atcctggggt
     1321 cgccaaccag gagccccatg aactcgaccg gaaacgactg ggactcactg aatggaacgg
     1381 aaaggagcaa cgaacaaccc aaagcgacta gtcgaagcgg aggggtcggt gccagtgtcg
     1441 ccgctgcaat cggcgaaccc gctaaccaag atcccacagg tccggtcgcg accggcactc
     1501 cgtcagaaag tgtgccaggt ggacattcac tggttgccca ggggccgctc cgggacaaac
     1561 cacgcgacca ccaacatgga ggagaaatga gaggtaaatg gatcgtggcg ctcgtagtcg
     1621 ggctcatcgt catggcgatc gccggcgccg tgctcgccac gcccggttcg gggttcaccg
     1681 ctgcacagca gtggaagggt gtgttcgggg acatcgatgt gcacctcgtc gacatgcccg
     1741 gccaccaggt caaagtcaag acgaatggcg tctccgacgt ctacgttacc cgcaatgcga
     1801 ttgcggtcga cgggcacagc ggctggcaca cgcacccggg cccaagtctg atcatggtga
     1861 ccgtcggcga gatcatggcc tacgacggcg acgatccgac ctgcacgccc acgctgtaca
     1921 cgacgggaga aggcttcgtc gacgagggcg ggggccacgt tcaccttctg cgcaacgaga
     1981 gtggcgcacc tgctgagacg gttgccatcc aattccttcc gaaggacgcg actcgacgga
     2041 tcgacatgcc cgatcccgga aactgctcct tctgagaagc accctgtgag gaggctcgcc
     2101 caacgggcct cctcatggcg gccgtcgtcg cctgctggtc gcgaccggac ctgtcgaatc
     2161 tcggtctcgc tcgactcgtg ggtaagcgaa
//
LOCUS       DATITW010000009         1611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_320948, whole genome shotgun sequence.
ACCESSION   DATITW010000009 DATITW010000000
VERSION     DATITW010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320948"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VLS86_00100"
     CDS             complement(<1..721)
                     /locus_tag="VLS86_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015378168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSO48917.1"
                     /translation="MSGPVQPLDATVDQGPLTRIEVKEGRSASVGGMSISRVLPTKGR
                     RTIGPWCFVDLILPPDFEDPHPMEVGPHPHTGLATVTWLFEGEALHTDSLGTEQPIRP
                     GQLNLMTSGHGIAHAELGTTTGIHGAQMWLAQPEGTRHGPSRFQHVEDLPSASLRGGE
                     AMVIVGELEGARSPAVLAWPTMGLDVRLHGGRVEIPTNPAYEHAVVPIDRAVKVDEAI
                     VEPGWLALVPVGAEMPPLEGGG"
     gene            complement(746..1360)
                     /locus_tag="VLS86_00105"
     CDS             complement(746..1360)
                     /locus_tag="VLS86_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HSO48918.1"
                     /translation="MSESTVLSVTPEALDMILQLRDQEPGDGEFALSIEVTGFRGPQF
                     TYELAFVGLDDMKPGWVLERHGDLALVFPGDDGEKLEGASLELTDQGLSMNNPNSPAP
                     PAMSAPAGDLSGPLVDQVQQVILEQVNPAIAAHGGGAELVSVDGTIAYLRLFGGCQGC
                     GLAQVTLKQGIERIILESIPALTQVVDVTDHASGEDPYYQSQKK"
     gene            1394..>1611
                     /locus_tag="VLS86_00110"
     CDS             1394..>1611
                     /locus_tag="VLS86_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO48919.1"
                     /translation="MTTAVVVLPSTTYRAADFVRAAEGLGIDLIVVSEQPPPFDMGDR
                     YLEIDCADAEKAAEAIVRLGDVAPIDGVV"
BASE COUNT          269 a          550 c          523 g          269 t
ORIGIN      
        1 cgccgccgcc ctcgagcggg ggcatctccg ccccaacggg caccagggcc agccaaccgg
       61 gctcaacgat ggcctcgtcc accttgaccg cacggtcgat cggcaccacc gcgtgctcat
      121 aggccgggtt ggtcgggatc tccacccgcc caccatgcag acggacatcg agccccatcg
      181 tcggccaggc gaggacggcc ggcgatctgg caccctcgag ctctccgacg atgaccatcg
      241 cctcgccgcc gcgcagtgag gccgatggca ggtcttcgac atgctggaag cgtgatggcc
      301 cgtgacgggt cccttcgggc tgggcgagcc acatctgtgc cccgtggatg ccggtggtgg
      361 tgcccaactc ggcgtgggcg atcccatgac cggaggtcat caggttcagc tgtccgggcc
      421 ggatcggctg ctcggtgccc aaagagtcgg tgtgcagggc ctcgccttcg aacagccagg
      481 tgacggtggc caacccggtg tgaggatggg ggccgacctc catgggatgt ggatcctcga
      541 agtcgggagg gaggatcagg tcgacgaagc accaggggcc gatggtccgg cgccccttgg
      601 tgggaagcac ccggctgatc gacatcccgc ccaccgatgc cgagcggccc tctttcacct
      661 cgatccgggt cagggggccc tgatcgacgg tcgcatcaag cggttgaacc ggcccgctca
      721 tcaggctcct cgactcggcc tacggctact tcttctggct ctggtagtag gggtcttcgc
      781 cggaggcatg atcggtgacg tccacgacct gggtcaaggc agggatcgac tcgaggatga
      841 tcctctcgat gccttgcttc agagtgacct gagccagccc acagccctgg caacccccga
      901 acaaccgaag ataggcgatc gtcccgtcga ccgacaccag ctcggctcca ccgccgtgag
      961 ccgcaatcgc cgggttgacc tgctccagga tgacctgttg cacctggtcg accagtgggc
     1021 cggagagatc acccgcgggc gccgacatcg ccggtggcgc cgggctgttc gggttgttca
     1081 tcgagaggcc ctgatcggtc agctccaggg aggcgccttc gagcttctcg ccgtcgtcgc
     1141 cgggaaagac gagcgccaga tccccatgtc gttccagcac ccagcccggt ttcatgtcgt
     1201 cgagcccgac aaaggccagc tcgtaggtga actgggggcc gcggaagccg gtgacttcga
     1261 tcgacaaggc gaactcaccg tcgcccggct cctgatccct caactggagg atcatgtcga
     1321 gtgcctctgg cgtgacactg aggacggtgc tctcggacat ctgccaggat ggtaacccct
     1381 ctccagagcc aagatgacca ccgccgtagt cgtactcccc agcaccacct atcgggccgc
     1441 ggacttcgtc cgcgccgccg aaggcctggg aatcgacctg atcgtcgtct ccgagcagcc
     1501 gcctcctttc gacatgggcg accgatatct cgagatcgac tgcgccgatg cggagaaggc
     1561 cgccgaggcg atcgtccgtc tcggcgatgt cgccccgatc gacggcgtgg t
//
LOCUS       DATITW010000010         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_280897, whole genome shotgun sequence.
ACCESSION   DATITW010000010 DATITW010000000
VERSION     DATITW010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280897"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..709)
                     /locus_tag="VLS86_00115"
     CDS             complement(77..709)
                     /locus_tag="VLS86_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HSO48920.1"
                     /translation="MLDGLVDWVTSVVETLGYGGVAFLVALENLFPPIPSEVVLPLAG
                     FVASSGDASLVGMIVAATIGSMVGAFLLYGIAAAVGPIRLRALVVRHGRWFGLDETDL
                     DKTEEWFDRKANSAVLICRCIPLMRSLISIPAGFRRMRLVPFTIFTLAGSLVWNVLLI
                     GAGYLLGEQWNKVEAPLELFQTLVLLVIGLALAWFIWRKIVRPRLRGAAG"
     gene            827..>2878
                     /locus_tag="VLS86_00120"
     CDS             827..>2878
                     /locus_tag="VLS86_00120"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HSO48921.1"
                     /translation="MDLSILAAEGGYQDFTLGAAEWIWLWVSAITAVVALLVGVVLSR
                     GVLKADKGTAAMQEIALAIQEGAMAYLRRQFKTIAVILVPLALIVFFTSTAVLKPDGS
                     EALSFAQSGAWRTTAFVLGGVLSGLTGFIGMSLAVRGNVRTAAAARSGSLPAALKVAF
                     RTGGVAGMFTVGLGLLGATIIIMVFQNTSSAILVGFGFGGSLLALFLRVGGGIFTKAA
                     DVGADLVGKVEQGIPEDDARNPATIADNVGDNVGDCAGMASDLFESYEVTLVASIILG
                     VAAFNSIGANPALGLIFPVVVRAIGVLASIAGVFQVRATPKDRSALAPINRGFLTAGL
                     LTVAGTLAVALLYVGNENGNEGWKVFGAVVTGLVLAQVVSRLTEYFTSTDEKPVQEIA
                     EATQTGPATTVLSGISSGLESSVWAIVAIAGALGVAIALGGGNIQFALYLVALTGMGM
                     LATTGVVVSEDTFGPVADNAAGIAEMSGEFHGEARSVMVSLDAVGNTTKAVTKGFAIG
                     SAVIAAVALFASFIETIAGELGLVAEGSDLFSLPETSMNVADPSTFIGLLIGGSVAFL
                     FSSLAIRAVGRTAAVVVQEVRRQFADGAIMRGERRPDYAPVIDICTAASLRELATPAL
                     LAVLTPVIIGFGISYFALGGFLAAAILTGQLMANFLSNSGGAWDNAKKYIEDGNYGGK
                     GS"
BASE COUNT          479 a          964 c          915 g          520 t
ORIGIN      
        1 gatggacttc gacctgtggg gtcatccacg attggcccag cttaaagcac gtgcataaac
       61 ccggttcgac ggccctttag cccgccgccc cacggagacg tggtcggacg atcttccgcc
      121 agatgaacca ggccagcgcc agcccgatca ccaacagcac gagtgtttgg aacagctcga
      181 gcggcgcctc gaccttgttc cactgctcgc cgagaaggta accggcgccg atcaacagca
      241 cgttccagac gaggcttccg gccagggtga agatggtgaa cgggaccaat cgcatccgtc
      301 tgaaccccgc cggaatcgag atgagggatc gcatgagcgg gatgcaacga cagatcaaca
      361 ccgcgctatt ggccttacgg tcgaaccact cctcggtctt gtccaggtcg gtctcgtcga
      421 gcccgaacca tctcccgtgc ctcaccacca gggcgcggag acggatcggc ccaacggcag
      481 ccgcgatgcc gtagaggaga aacgccccga ccatcgagcc gatggtggct gcgacgatca
      541 tccccaccag ggaggcatca ccactcgacg ccacgaaacc cgccaacgga agcaccactt
      601 ctgacgggat cggcgggaag aggttctcga gggcgacgag gaaggcgaca cctccgtatc
      661 cgagggtctc caccacggag gtcacccagt caacaagtcc gtcgagcaac tgcggtcctc
      721 ctgttcaagg gctcgtatgt gccgggacca cccggcctcg ggagattgtc gacaggaaac
      781 ggcctccact acagtctcgg cccgcccccc acggaggata actctcttgg acctgtcgat
      841 cttggctgcc gaaggcggct atcaggactt cacgctcggc gccgccgagt ggatctggct
      901 ttgggtatcc gccatcaccg cggtcgtcgc tctcttggtc ggcgtggtcc tgtcgcgcgg
      961 agtcctcaag gcggacaagg gcacggcagc gatgcaggag atcgcccttg ccattcagga
     1021 aggggcgatg gcctatctcc gccgccagtt caagaccatc gcggtcatcc tcgtcccgct
     1081 ggctctcatc gtcttcttca cttcgaccgc cgtgctcaag cccgacggga gcgaagcgct
     1141 gagttttgcc caatcagggg cgtggagaac caccgcgttc gtcctcggag gtgtcctctc
     1201 cggattgacc ggtttcatcg gcatgtcgct ggccgtgcgg ggcaacgtcc gcaccgcggc
     1261 cgcggccagg tctggctccc tgcccgccgc gctcaaggtg gcgtttcgga cgggcggcgt
     1321 ggccgggatg ttcaccgtcg gcctcggtct gctcggggcc accatcatca tcatggtctt
     1381 ccagaacacc tcctcggcga tcctggtcgg ctttgggttc ggaggatcgc tgctcgccct
     1441 cttcctccgg gtcggtgggg ggatcttcac caaggcggcc gatgtgggcg ccgacttggt
     1501 gggcaaggtc gaacagggga ttcccgagga cgacgcgcga aatccggcca ccatcgccga
     1561 caacgttggt gacaatgtcg gagactgcgc cggaatggcc tcggacctgt tcgagagcta
     1621 cgaggtcacc ctggtcgcct cgatcatcct cggcgtggcc gccttcaact cgatcggcgc
     1681 caaccccgct ctcggtctca tcttcccggt ggtggtgaga gccatcggag tcctggcctc
     1741 gatcgcgggc gtgttccaag tgagggccac gccgaaggac aggtcggccc tggccccgat
     1801 caaccgcggg ttcctcaccg ccgggctcct caccgtcgcc gggaccctgg cggtggcctt
     1861 gctctacgta ggcaacgaga acggaaacga gggctggaag gtgttcggtg ccgtggtcac
     1921 gggcctcgtc ctggctcaag tggtcagccg gctcaccgag tacttcacct cgacggatga
     1981 gaagccggtc caggagatcg ccgaagccac ccagaccggc ccggctacaa ccgttctctc
     2041 cggcatctcc tcgggcttgg agtcgtcggt gtgggccatc gtggccattg ccggcgctct
     2101 cggtgtcgcc atcgccctcg gtgggggcaa catccaattc gccctctacc tggtggcgct
     2161 gaccgggatg ggaatgctcg ccaccacagg tgtcgtggtt tccgaggaca cgttcggccc
     2221 cgtggccgac aacgcggccg gcatcgccga gatgtcgggg gagttccacg gtgaggcgcg
     2281 ctcggtgatg gtcagcctcg atgccgtggg caacaccacc aaggcggtga ccaagggatt
     2341 cgccattggc tcggcggtga tcgcggccgt agctctcttc gcctcgttca tcgagaccat
     2401 cgccggagag ctgggtctgg tcgccgaggg ctcggatctc ttcagtctcc ccgagacctc
     2461 gatgaacgtg gccgatcctt ccaccttcat cgggcttctc atcggaggct ccgtggcgtt
     2521 cttgttctcc agtctggcca ttcgggccgt gggccgcacc gcggcggtcg tggtccagga
     2581 ggtgcgccgc cagttcgccg acggcgccat catgagaggc gagcgtcgcc ccgattacgc
     2641 accggtcatc gacatctgca ccgcggcctc cctgcgtgag ctggcgacgc cggcgctcct
     2701 ggcggtgctc accccggtga tcatcgggtt cgggatcagt tacttcgccc tcggtggctt
     2761 cctggcagcc gcgatcctca ccgggcagct catggccaac ttcctctcca actccggtgg
     2821 ggcctgggac aacgccaaga agtacatcga ggacggcaac tacggtggca aaggctca
//
LOCUS       DATITW010000011         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_761736, whole genome shotgun sequence.
ACCESSION   DATITW010000011 DATITW010000000
VERSION     DATITW010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761736"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1992
                     /gene="nosZ"
                     /locus_tag="VLS86_00125"
     CDS             106..1992
                     /gene="nosZ"
                     /locus_tag="VLS86_00125"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HSO48922.1"
                     /translation="MFRGLIALVLLGLAACAPETTPVAGAKGGARLADAREAAQRTFV
                     EPGKLDDYYLFYSGGHSGQVYVAGVPSMRHIATIPVFAPYPATGYGFDEESKKLLGSY
                     TWGDMHHPALSKVKGDYDGRWLFVNDNANNRLARIDLRDFKTKQILGPIPNSSGNHGS
                     AFVTENTEYALVASRFSIPVPRGKYAPLSKYATEYHGVLSGVRIDSVTGAMSMGWQIL
                     TPPFNWDLAATGREISKDWAVCSSYNTERAIGRLEVTASQKDRDYIAFVNWRKAAAAA
                     AAGKGRMIDGVRVLNPTEVPGMMYLVPLAKSPHGADFSPDGRYVVGSGKLAPYVTVFE
                     FEKFLKAVESGVTIGEEDGIPVLKHEDIRVAEVPVGLGPLHTQFDDQGYAYTSLFIES
                     AIVKWKLGPFNGNDSALVVAKIPVHFSVGHLVTAHGDTRHPHGKYLVSMNKLSKGRHL
                     NVGPSQPESSQLIDITGTGMTMLYEAYTEPEPHFAQLVKADLLKPIEVYLKAENKDPN
                     AIWAAADGKATRKGNRVEVKLFSVRSYFAPSAIEVQQGDSVIIHVTNAEQERDMLHGL
                     GIAGYNINLVMDPGETKTVRFKANKPGVWPFYCTNFCSAMHQEMQGYLLVKPRAAASE
                     VARK"
BASE COUNT          411 a          678 c          676 g          362 t
ORIGIN      
        1 cggtgattca catcctcacc cagctccacg aggatgccga ggacgtggca gagaccaaga
       61 aggacgattg atatggcaca gtctcatcca actaaggagc gtcccatgtt ccgaggcctt
      121 atagcgctgg tgctgcttgg gttggccgca tgcgcgcccg aaaccacccc ggtggccggt
      181 gcgaagggag gcgctcgcct ggcagatgcc cgcgaggccg cgcagcgcac cttcgtggag
      241 ccggggaagc tcgacgacta ctacctgttc tacagcggtg ggcactctgg ccaggtctat
      301 gttgccggcg tcccctccat gcgtcacatc gccaccatcc cggtattcgc cccctatccc
      361 gccaccggtt acggcttcga tgaggagtcg aagaagctgc tcggcagcta cacctggggc
      421 gacatgcatc acccggcgct ctccaaggtg aagggtgact acgatggccg ctggctgttc
      481 gtcaacgaca atgccaacaa ccgcctggcc cggatcgatc tgcgcgactt caagaccaag
      541 cagatcctcg gccccatccc gaactccagc ggcaaccacg ggtcggcctt cgtgaccgag
      601 aacaccgagt atgcgctggt ggcgagtcgg ttctcgatcc cggtcccgcg ggggaagtat
      661 gcaccactga gcaagtatgc gaccgaatac catggcgtcc tgagtggtgt cagaatcgat
      721 agcgtcacgg gggcgatgtc catgggctgg cagatcctca ccccgccctt caactgggac
      781 ctggccgcga cagggaggga gatctcgaaa gactgggcag tgtgtagctc ctataacacc
      841 gaacgggcca tcggccggct ggaggtgacc gcgagccaga aggatcggga ctacatcgcc
      901 ttcgtgaact ggcgcaaggc ggccgcggcc gcggcggcag ggaaaggcag gatgatcgac
      961 ggtgtgcggg tgctcaaccc gaccgaggtt ccgggcatga tgtacctggt tccactcgcc
     1021 aagagcccgc acggtgcgga cttctcgccc gacggccgct acgtcgtggg cagcggaaag
     1081 ctggcaccgt acgtcacggt ctttgagttc gaaaagttcc tcaaggctgt ggagagcggc
     1141 gttacaatag gagaggaaga cgggatcccg gtcctcaagc acgaggacat ccgggtggcc
     1201 gaagtgccgg tgggcctggg tccgctgcac acccagttcg acgatcaggg ttacgcctac
     1261 acgtcgctct tcatcgagtc ggcgatcgtc aaatggaagc tcggcccttt caacggcaac
     1321 gattcggcgc tcgtggtggc gaagatcccg gtccacttct cggtcggcca cctggtgacc
     1381 gctcacgggg atactcggca cccccacggg aaatacctgg tctcgatgaa caagctctcg
     1441 aagggtcgcc atctcaacgt gggcccgtcc cagccggagt cgagccagct gatcgacatc
     1501 accggcaccg ggatgacgat gctgtacgaa gcctacaccg agcccgagcc ccatttcgcc
     1561 cagctggtca aggcggacct gctcaagccg atcgaggtct acctcaaggc ggagaacaag
     1621 gatcccaacg ccatctgggc cgcggcggac ggcaaggcca cccgcaaagg caaccgggtg
     1681 gaggtcaagc tcttctcggt ccggtcctac tttgcgccta gcgcgatcga ggtccaacaa
     1741 ggcgactcgg tcatcatcca tgtcaccaac gccgagcagg agcgggacat gctccatggc
     1801 ctcgggatcg ccggctacaa catcaacctg gtgatggacc cgggtgagac caagacggtg
     1861 cgcttcaagg cgaacaagcc cggcgtgtgg ccgttctact gcaccaactt ctgctcggcg
     1921 atgcaccagg agatgcaggg ctatctgctg gtcaaacccc gcgccgcggc atccgaagtg
     1981 gcgcggaagt aggcccgggt gaagaccagg tcgctcacgc tctggctcga tcgggttctc
     2041 tccccccggg cgcggctcgc cgtgctcggg gcgggggtcc tcctgctggt ggcggttttc
     2101 tttcccctgt gggagatcac catgctg
//
LOCUS       DATITW010000012         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_521383, whole genome shotgun sequence.
ACCESSION   DATITW010000012 DATITW010000000
VERSION     DATITW010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521383"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VLS86_00130"
     CDS             complement(<1..993)
                     /locus_tag="VLS86_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSO48923.1"
                     /translation="MLGGFAAAAAIILFVTAHEAGHFIAAKTVGMKATEFFFGFGPKI
                     WSTQKGETEYGFKWIPLGGYVRIVGMNPLEEVSPADLGRTYREKKFWEKSVVVLAGVG
                     MNFLIAFVIFYGLVVANGVSEPLPVVDEVIQTTEVDGESSPTPAAQAGLEEGDVIVSI
                     GDVSIDDWDDVPLALTETGAGATTVVVNRDGRMESLDTVLVERGADEGGAATVFLGIA
                     PAIETTEPGVIGSIGVAGNQVWQGVGFTFQAIGDMVKPSSLAQYVGVLAGDTEVDEEI
                     RPVSLIGIVNIGTQTESVAAFLGILAFVNVILGTLNLLPLSPLDGGHFAVAVYEK"
     gene            complement(1024..>2151)
                     /gene="dxr"
                     /locus_tag="VLS86_00135"
     CDS             complement(1024..>2151)
                     /gene="dxr"
                     /locus_tag="VLS86_00135"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HSO48924.1"
                     /translation="VLGATGSIGEQTIEVAAGLGLEVSALAARSPSAKLAALAAEHPS
                     ARVIVAGGSSEERDDFRRWVGREVEFGSEAVADAARTTDATIVNGIVGAAGLRATVAG
                     LEAGNRVALANKESLVAGGPVVKQALADHGGELIPVDSEHSALFQCMVGEDPAGVSRL
                     ILTASGGPFLGRSRESLGEVTPAQALAHPTWDMGRRISIDSATLFNKGLEVIEAHFLF
                     GVPYERIVVVIHPQSILHSAVEFVDGSWKGHIGHPDMRIPIQYALTTPGRARSAVAPF
                     DLAGRELSFEEPDRATFPALDLAYRAGGQGGSSPAVLNAADEVAVEAFLQGRLGFLGI
                     ADVVSSTLDLVAWRPLESVEEVLEADREARETAASLIAGAC"
BASE COUNT          372 a          766 c          681 g          332 t
ORIGIN      
        1 cttctcgtac acggccacgg cgaagtgccc gccgtccagg ggggagagtg gaagcaggtt
       61 gagcgtgccc aggatcacgt tgacaaacgc caggatcccg aggaaggcag cgacagactc
      121 ggtctgggtc ccgatgttga cgatgccgat caggctcacc gggcgaatct cctcgtcaac
      181 ctcggtgtca ccggccagca ctcccacgta ctgggcgaga ctcgacggct tcaccatgtc
      241 cccgatggcc tggaaggtga acccgactcc ctgccacacc tggttgccgg ctacgccgat
      301 cgagccgatg acaccgggct cggtggtctc gatagccggc gcgatcccga ggaagaccgt
      361 ggctgcgccg ccctcgtcgg caccgcgctc gaccagcacg gtgtccaggc tctccatccg
      421 gccgtcgcga ttcacgacga cagtcgtggc accggctccg gtctcggtga gagccagggg
      481 aacgtcatcc cagtcgtcga tcgacacgtc cccgatggag acgatcacat cgccttcttc
      541 cagcccggcc tgcgccgccg gggtcgggct gctctcccca tcgacctcag tggtctggat
      601 cacctcatcg acgacgggca gcggctcgga gacgccgttg gcgacgacga gcccgtagaa
      661 gatcacgaag gcgatgagga agttcatccc cacacccgcc aggaccacca cgctcttctc
      721 ccagaacttc ttctcgcgat aggtgcgacc gaggtcggca ggtgacacct cctcgagcgg
      781 gttcatcccg acgatgcgga cgtacccgcc cagcgggatc cacttgaagc cgtactcggt
      841 ctcgcctttc tgagtcgacc agatcttggg gccgaagccg aagaagaact ccgtggcctt
      901 catgcccacc gtcttggccg cgatgaagtg accggcttcg tgggcggtga cgaaaaggat
      961 gatggctgcc gcggcagcaa agcctccgag catcctcaca cgctacccga ggccaccctc
     1021 tactcagcag gctccggcga tgagcgaggc cgccgtctcc cgggcctcac ggtcggcctc
     1081 gaggacttcc tccaccgact cgagcggtcg ccacgccacc aggtcgaggg tggatgacac
     1141 cacgtctgcg attccgagga accccagtcg tccctggagg aaggcctcga ccgcgacttc
     1201 gtcggcagcg ttgagcactg ccggtgacga gcctccctgc ccgcccgccc gataggcgag
     1261 atcgagggcg gggaaggtgg cgcggtccgg ctcctcgaag ctcagctcgc gcccggcgag
     1321 atcgaacggc gcgaccgccg accgggcccg gcccggcgtg gtgagggcgt attggatcgg
     1381 aatcctcatg tcggggtgcc cgatatggcc cttccacgag ccatcgacga actcgaccgc
     1441 cgaatgcagg atcgactgag ggtggatcac gaccacgatc cgctcgtagg ggaccccgaa
     1501 caagaagtgg gcctcgatca cctccaggcc cttgttgaac agggtggccg aatcgatgct
     1561 gatcctccgt cccatgtccc aggtcgggtg agccagagct tgggcgggtg tcacctcgcc
     1621 gagcgactcc ctcgacctgc ccagaaaggg cccgccggag gcggtgagga tcaggcgcga
     1681 cacgcccgcc gggtcctcac cgaccatgca ctggaagagg gcagaatgct cactgtccac
     1741 cgggatcagc tcacccccgt ggtcggccag ggcctgcttc accaccgggc ctccggcaac
     1801 gagactctct ttgttggcca gcgccacccg gttgcctgcc tccagaccgg ccactgtcgc
     1861 ccggagcccg gccgccccca cgatcccgtt gacgatcgtc gcgtccgtgg ttcgggcggc
     1921 gtcggccacc gcctcggagc cgaactcgac ctcccgcccc acccatcgtc gaaagtcgtc
     1981 gcgctcctcg gaggagccgc cggcgactat cacccgtgcc gagggatgct cggcggcgag
     2041 cgcagccagc ttggccgaag gggatcgggc agccagcgcc gacacctcga gaccgagacc
     2101 ggctgcgacc tcgattgtct gttccccgat cgatccggtg gccccgagaa c
//
LOCUS       DATITW010000013         1647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_40861, whole genome shotgun sequence.
ACCESSION   DATITW010000013 DATITW010000000
VERSION     DATITW010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40861"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..659)
                     /locus_tag="VLS86_00140"
     CDS             complement(69..659)
                     /locus_tag="VLS86_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO48925.1"
                     /translation="MTEGTSRTDPLDMVKRYAILAYGAFGTMLDVGLMVLGSLLVGLS
                     IAVLLAGFQIVNVVQELSTGGMLASALVLAIVGLFCLGVASEGPLGRGRKLVGFQLWE
                     VGIGRTVAVFLVGFIGLLVYGFISRVLEDLPEPLHKGAEGLRAVGIAGMVVMPLIGVP
                     LSLLVRWAPNAPTWLDKAEIPVLFVVWAIATMAIFA"
     gene            complement(656..>1647)
                     /locus_tag="VLS86_00145"
     CDS             complement(656..>1647)
                     /locus_tag="VLS86_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HSO48926.1"
                     /translation="EGTRAEIVAQRPSLSHRLRSFLLGSVLGSRRSLGFGTGALSLAQ
                     MSGVTKAAPKRWRRLSSGMRQLRGRPGSVIGHSGGLGAAGRVAVLAGCIQDQWFQPVN
                     HAAIELLEMAGFRVDVPAGQTCCGALAAHDGKAPEAHRLAERNTPVFTGFDLVVATAA
                     GCSAHLRDYSHWAEDGEGVGSTSLDITVAVARAIEGGLLPKVSSVSGRIGIQDPCHLR
                     HAQKVTVEPRLILEAAGYEVVEIDPTGMCCGAAGLYTILQPEASQELGNRKASQVRST
                     GVSKVASANPGCEMQLRSALGDGFEIAHPIEWYLQAVKAEKRGGIVAALEESR"
BASE COUNT          317 a          586 c          506 g          238 t
ORIGIN      
        1 agaggcgctg atcagagaag gattcaccac ccgccggttc ttgatctacg cgaagagcct
       61 ctgagccttc aggcgaagat tgccatggtg gcgatcgccc agaccacgaa caggaccggg
      121 atctcggctt tgtccagcca ggtcggggcg ttgggggccc agcggaccag tagtgagagc
      181 ggcaccccga tcaggggcat gaccaccatc ccggcgatcc ccaccgcccg caatccttcg
      241 gcgcccttgt ggagaggctc gggaagatcc tcgagcaccc tgctgatgaa cccgtacacc
      301 aacaacccga tgaacccgac caggaacacc gccacggtgc ggccgatccc cacctcccac
      361 aactgaaagc cgaccagctt ccggccccgc ccgagcgggc cttcggaagc caccccgaga
      421 cagaagaggc cgacgatcgc caggaccaga gccgaggcga gcatgccacc ggtggacaac
      481 tcctggacga cgttgacgat ctggaacccg gcaagcagca ccgcgatcga aagcccaacc
      541 agaaggctcc ccaggaccat cagccccacg tcgagcatgg tcccgaacgc cccataggcg
      601 aggatggcgt aacgcttgac catgtcgagg gggtcggtgc gactggtgcc ttcggtcaac
      661 gtgactcctc caaggcggcg acgataccgc cccgcttttc cgctttgacg gcttgcagat
      721 accactcgat tgggtgggcg atctcaaacc cgtcgccgag cgcgctgcgc aactgcatct
      781 cgcaaccggg attcgccgac gccaccttgc tcaccccggt tgaacgcacc tgggatgcct
      841 ttcggtttcc gagctcctgg gaggcctcgg gttggaggat ggtgtagagc ccggccgcgc
      901 cacagcacat cccggtgggg tcgatctcga ccacctcgta gccggccgcc tccaggatca
      961 ggcgcggctc gacggtcacc ttttgtgcgt gacggagatg acacggatcc tggatcccga
     1021 tccggcctga gaccgagctc accttgggca gcaaaccgcc ttctatggca cgggctacgg
     1081 cgaccgtgat gtcgagagat gtggacccga ccccctcgcc atcctccgcc cagtggctgt
     1141 agtcccggag atgcgccgaa caaccggccg cggtggccac gaccaggtcg aaaccggtga
     1201 acaccggggt gttgcgctcg gcgagccgat gggcttccgg cgcttttccg tcgtgggcgg
     1261 cgagtgcgcc acaacaagtc tgacccgccg gcacatcgac ccgaaatccg gccatctcca
     1321 acagttcgat ggcggcgtga ttcaccggct ggaaccattg gtcctggata cacccggcaa
     1381 gcacggcgac cctccccgcc gcgcccaacc caccggagtg cccgatcacc gatccgggcc
     1441 ggccacgcaa ttgacgcatc cccgacgaca accggcgcca ccgtttgggc gcggccttgg
     1501 tcaccccgct catctgggcg aggctgagcg ccccggtccc gaaaccaagc gaccgccggg
     1561 agccgagcac cgacccgagc aggaacgacc ggagacgatg tgacagcgac ggccgttggg
     1621 cgacgatctc ggcccgggtg ccctcca
//
LOCUS       DATITW010000014         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_161009, whole genome shotgun sequence.
ACCESSION   DATITW010000014 DATITW010000000
VERSION     DATITW010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161009"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1051)
                     /locus_tag="VLS86_00150"
     CDS             complement(161..1051)
                     /locus_tag="VLS86_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSO48927.1"
                     /translation="MPIERLRITVLRRLDGLLAGDHSGLFPGHGTERGEARPYVPGDD
                     PRHIDWAVTARTREPHVRDTIADHELQVWLVLDTSSSMGFGTGRATKHEVAWSAAGAL
                     ALLAVSSGNHVGAVRTTTDGPRSRPQILPARGGSTHAGAVLAALHRSPVEGETGDLRR
                     AIEMIRRTARRRGMAVVVSDFLGTATWERSLRALSHRHEVIAVEVTDPRETDLPDVGL
                     ISVVDPETGRRRLVDTAKPTTRQRYGELAGRRREELAARLKSAGADHLVLRTDRDWVL
                     DFVRFVSGRKARLMAAGRSR"
     gene            complement(1129..2166)
                     /locus_tag="VLS86_00155"
     CDS             complement(1129..2166)
                     /locus_tag="VLS86_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSO48928.1"
                     /translation="MPFPIPPTGGEPQSGLLGIRLREVMAEMQTVIVGQERVLERVLV
                     ALLAGGHCLLEGVPGLGKTLTVSTLAKALGGTFTRIQFTPDLLPSDIVGTRIYRPSTE
                     RFDVELGPVFANFVLADEINRAPAKVQSALLEAMAERQVSIGGTTYPLPSPFLVLATQ
                     NPIESEGVYPLPEAQRDRFLMRIPVDYPTPGEEHEIVDRSRRQTTAAASLLSTEDVAS
                     LQSAANAVYVERSVQDYAIRLVLATRHPSEHGVKIDGLLAYGASPRASIGLISAGRAL
                     ALLRGRDHLLPQDVYDIAYDVLNHRLVLSFDAVADGVSVDDVLIEVLTTVVAPRVSSS
                     QLFEQRSSVGV"
BASE COUNT          410 a          784 c          792 g          339 t
ORIGIN      
        1 cccgggcgat ccggggcgac ctgatcgagc agatcgatcg tggtgaaccg gagcgcatag
       61 cggcgtcgtc tcgcctggac gacgaggtag gcgatcgcca gcgccaccgg gagcaacagg
      121 agaaccagcc gccccggagc gaggaacccg aagtcgccga tcatcgcgac ctccccgccg
      181 ccatcaacct cgccttgcgc cccgagacga agcggacgaa atcgagcacc caatctcgat
      241 cggttcgcag caccaggtga tcggcaccgg cgctcttgag tcgggcggca agctcttccc
      301 ggcggcgccc ggccagctcg ccgtagcgct gccgcgtcgt gggcttcgcg gtgtcgacca
      361 gccggcgccg gccggtctcg gggtcgacca ccgagatcaa cccgacgtcg ggaaggtcgg
      421 tctcgcgagg gtcggtcact tcgaccgcga tcacctcgtg acgatgtgac agcgcacgca
      481 gcgacctctc ccacgtcgcc gtccccagga agtcggagac caccacggcc atgccgcgac
      541 gacgggccgt gcgccggatc atctcgatgg cgcgacggag gtcccccgtc tcgccttcaa
      601 ccggcgaccg atggagagcg gcgagcacgg caccggcatg ggtcgaaccg ccgcgggcgg
      661 ggaggatctg cgggcgtgac cggggaccgt ccgttgtcgt ccgcaccgcg cccacgtggt
      721 tgcccgacga aacggcgagc agggcaagag cccccgccgc cgaccaggcc acctcgtgct
      781 tggtggcacg gccggtcccg aaccccatcg acgacgaggt gtcgagcacc agccatacct
      841 gaagctcatg atcggcgatg gtgtcccgga cgtgtggttc tcgagtccgg gccgtcaccg
      901 cccagtcgat gtgacgggga tcgtcaccgg gcacataggg ccgcgcctcg ccgcgctcgg
      961 tgccgtgacc gggaaacagg ccggagtggt ctccggcgag gagaccgtcg agacggcgca
     1021 gcacggttat ccgcaacctc tcgatcggca cctgcgacgg cgaggtcagt cgcgacgacc
     1081 tggggatcgc ctctacgccg gtcgggggac cggttctgcc tcgcatgctc agacccccac
     1141 tgaggatcgc tgctcgaaga gctgcgagga cgacacccgg ggggcaacca ccgtggtgag
     1201 aacctcgatg agcacgtcgt ccacgctcac cccgtcggcc actgcgtcga aggagagcac
     1261 cagccgatgg ttgagcacgt cgtatgcaat gtcgtagacg tcctggggga ggagatggtc
     1321 gcgccctctg agcagggcga gggccctgcc ggcgctgatc aggccgatgc tcgcccgggg
     1381 gctggcgccg taggcgagaa ggccatcgat cttcaccccg tgctcggagg gatggcgggt
     1441 ggccagcacc agtcttatcg catagtcctg caccgatcgc tcgacataga cggcattcgc
     1501 cgcggactgc aggctggcca catcctcggt ggagagcaga gatgccgcag ccgtcgtctg
     1561 gcgacgggaa cggtcgacga tctcatgctc ctctccggga gtcgggtagt ccaccgggat
     1621 cctcatcagg aaacgatctc gctgtgcctc cggaagcgga tagacaccct cggattcgat
     1681 cgggttctgt gtggcgagca ccaggaaggg tgaaggcagc gggtaggtgg tgcctccgat
     1741 ggagacctgt cgctccgcca tggcctcgag caatgcggac tgcaccttgg ctggggcccg
     1801 attgatctca tcggcgagca cgaagttggc gaacaccggg cccagctcga cgtcgaagcg
     1861 ctcggtggaa ggacggtaga tccgggttcc gacgatgtcc gagggcaaca ggtcaggggt
     1921 gaactggatc cgggtgaagg taccgccgag agccttggcc agggtcgaga ccgtgagggt
     1981 cttgcccagg cccggaactc cttcgagcaa gcagtggccc ccggccagca gcgcaaccag
     2041 gacacgctcg agaacgcgct cctggcccac gatgaccgtt tgcatctcgg ccatgacctc
     2101 ccggagccgg attccgagaa gcccggactg aggctctcca ccggtcggcg gaatcgggaa
     2161 gggcatcatg cgtctccctc ctcacccggt atcggaaccg acatgcgcaa cagctcatcg
     2221 agaagctgct ccagctcggg gggaatggtc cccgggccga acatctcctc caacatctcc
     2281 tgcagaccgg gcaggtcctc gagcccctga ccgcctccgg ggcca
//
LOCUS       DATITW010000015         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_481473, whole genome shotgun sequence.
ACCESSION   DATITW010000015 DATITW010000000
VERSION     DATITW010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481473"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..768
                     /locus_tag="VLS86_00160"
     CDS             400..768
                     /locus_tag="VLS86_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSO48929.1"
                     /translation="MRLLVVEDDAKVGGFLEQGLREEEFEVWRGYDGEEAVSMALNQH
                     YDLILLDYMLPKRNGPQVVKALRDQGCLTPILMLTARDAPEDIQLCFASGANAYLTKP
                     FRFADLLDQIQRLAGARREG"
     gene            837..>2167
                     /locus_tag="VLS86_00165"
     CDS             837..>2167
                     /locus_tag="VLS86_00165"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSO48930.1"
                     /translation="MALLGLALWGYLGVRSTLMRQLDRSLESSARLQVDNLAANGNFD
                     AHEHESVELNHFIENGNRLVVIRDSTGRILTSNTALARALPLETLSFRKSREGSRALI
                     TQQWHGQGFRSIYLPVPRTRRTEAAVVQVGASLISLDASLSTVVLQMVGTVVLVTLAT
                     AIGAGWLAGSAVAPVHQITAQARAITGEVAGQRITAHANVSEFHGLTEVLNAMVARLE
                     RASQWHRRIIRDLGHDLRTPITAMRAGVEVALWSERSPDDYRRILASTLEEIDRLSLI
                     GDALVLLGRFQAGEVTLDLAEIDARVLARKAVAEARDGVGSHPVTLVQPAEPVPIRAD
                     VRLTRMVLDQLLDNARRYTPPGSPIEVTVRAGETGAVITVEDNGTGVTDEVLPHLFEP
                     FYRSDMARAREGGPGLGLTATASIVTLHGGTVEANRGSGGGLRVTVTLPAAA"
BASE COUNT          409 a          671 c          699 g          388 t
ORIGIN      
        1 ccggctgcgc cagcatgagc agccaggcga tgctcacgaa gatggcgagg ccaatggccc
       61 gatggtgtct cgagagaacc ggtgccaccg acctcacaga cctcattgca ggctcccctg
      121 gagcttccga cctcacctgg aacaccaccc taccactgtg acgatgacac cgctccattc
      181 aaggcgccgg gaaagggcca tgaaggcctc ttcacgagac ctccacctcg gcttcacggg
      241 cagcgcacta gactctccat ccatggatag gtcgatcaca tcataagcta gtggcggcgc
      301 ctcaacgaca gttcgcatgg gtgcccattg caccggggag ctgccccgcc accaagccgc
      361 tggtgtgcaa gcaggggcat cgaacaggtt ggagcgaaca tgcgacttct cgtcgtggaa
      421 gacgacgcca aggtgggcgg atttctggag caaggactca gggaagagga gttcgaggtt
      481 tggcgcggat acgatggcga agaggcagtc tccatggcgc tcaatcagca ctatgacctc
      541 atcctgctcg actacatgct gcccaagcgg aatggcccgc aagtggtcaa ggcgttacgc
      601 gaccagggtt gtttaacgcc gatcctcatg ttgaccgcca gggacgcgcc cgaggacatc
      661 cagctctgtt tcgcctcggg ggccaacgcc tacctgacga agccattccg gttcgctgat
      721 ctgcttgatc agatccagag gctcgcggga gcccggcgcg agggctaatg gcacgcacga
      781 cgcgttcgtt cagtctgcag cggacgctcg cagtccggtt ctccttgaca atgttcgtgg
      841 cattgctcgg cctggcgctc tggggctatc tcggggttcg cagcacgctg atgagacagc
      901 tcgatcgctc cctggagagc tccgcgcgtc tccaggtgga taaccttgcc gcgaacggca
      961 atttcgacgc gcatgagcat gagagcgtag agttgaatca cttcatcgag aacggcaacc
     1021 gtcttgtcgt catccgtgac tccacaggcc ggatcctcac cagcaacacc gctttggctc
     1081 gtgctcttcc gttagaaacg ctcagcttcc ggaagtcccg ggagggcagc cgtgcgctga
     1141 tcacgcaaca atggcacggc cagggctttc gttcgatcta cctcccggtg ccgcgaacga
     1201 ggcgaaccga agccgcagtg gtccaggtgg gagcatccct gatatctctc gacgcgagcc
     1261 tgagcaccgt agtcctccag atggtcggga cagtggttct ggtcacgctt gctaccgcca
     1321 taggtgcggg ctggttggct ggttctgccg tggccccggt gcaccagatc acggcacagg
     1381 cacgtgcgat taccggagag gtcgccgggc aacgcatcac tgcccacgcc aacgtgagcg
     1441 agttccacgg actcactgag gtgctcaacg ccatggtggc tcgactggaa cgtgcctcgc
     1501 aatggcatcg gcgaattatc cgggatctcg gccacgacct gcgcacgccg atcaccgcga
     1561 tgcgggccgg cgtcgaggtc gccctctgga gtgaacggag cccggacgat tatcggcgaa
     1621 ttctggcgag cacgctcgag gagatcgatc gcctcagcct gatcggtgat gcgctggttc
     1681 tcctgggacg gttccaggcg ggagaggtta cgctggacct ggccgaaatc gacgctcggg
     1741 tcctggcgag gaaagcggtg gctgaagcgc gggatggggt tggttcacac cccgttaccc
     1801 tggtgcagcc cgcggagccg gtgccgattc gggccgatgt tcgtttgacg aggatggttc
     1861 tggatcagct gctcgacaac gcgcgacggt acactcctcc gggctccccc atcgaggtca
     1921 ccgtgcgcgc cggggaaacg ggagcggtga tcacggtcga ggacaacggc acaggggtca
     1981 cggacgaggt cttgccccat ctgtttgagc cgttctatcg cagcgacatg gcgagagcac
     2041 gcgagggcgg gccggggctc gggctcaccg caaccgcctc aatcgtcacg ctgcacgggg
     2101 gaaccgtgga ggccaatcga ggatcaggag gcgggcttcg agtcaccgtc acgttgcctg
     2161 cggcggc
//
LOCUS       DATITW010000016         1609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_241271, whole genome shotgun sequence.
ACCESSION   DATITW010000016 DATITW010000000
VERSION     DATITW010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241271"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VLS86_00170"
     CDS             <1..805
                     /locus_tag="VLS86_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HSO48931.1"
                     /translation="LFDEGKIALDDVEAMVNQFVLLRSGASRSALARVVQAEPMVIVE
                     RVPTHIRAFGVEPKNVRQTVALHQLLDPDIPAVSLLGMAGTGKTFLALAAALEQVLEQ
                     GRYRRVSVYRPLVAVGRQEVGYLPGDLNEKLAPWMAAVHDNLYALFSDAGVDGAKHAI
                     EELVERGELEMAAVTYLRGRSITGEFVIIDEAQNLELPTLKVILTRMSRDSKVVFCGD
                     LTQVDNPYISPFGGLAALIEKFKGSSLFGHVTLEKSVRSPLAEMAATIL"
     gene            complement(802..>1609)
                     /locus_tag="VLS86_00175"
     CDS             complement(802..>1609)
                     /locus_tag="VLS86_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSO48932.1"
                     /translation="ITTTAVAVMVVVRQNLVVREKRELLEQQRDDLVASVSHELRTPL
                     TGIQGYAQILGESWEELSPEERDEMLETINTQANHLGHIVTDLIDVARDRLQNVELKL
                     VDYNAADLAREAVAVGGGGKGIRVEADAEARVWAEPDRIRQVLVNLLTNAVRYGRDQI
                     LVTVARSGPVVLFAVHDNGDGVPAKHQSEMWERFERGLHQQNTSVPGSGIGLSVARDL
                     VSAHGGTIEYRTSERLGGACFEFTIPVPRPGVGELVSAGAAGRQTGSGRN"
BASE COUNT          254 a          524 c          526 g          305 t
ORIGIN      
        1 cctcttcgac gaaggcaaga tcgccctcga cgatgtggag gcgatggtca accagttcgt
       61 cctgctccgg tcgggcgctt cgcgttcggc cctggccagg gtggtgcagg cggagccgat
      121 ggtgatcgtc gagcgggtcc ccacccacat ccgggcgttc ggcgtggagc ccaagaacgt
      181 gcggcagacg gtggcactcc accagctgct cgatcctgac atcccggcgg tctccctgct
      241 gggaatggca ggaaccggga agacttttct cgccctggcc gcggctctgg aacaggtatt
      301 ggagcaaggt cgctaccgac gggtgtcggt ttaccgcccg ctggtggcgg tggggcgcca
      361 ggaggtcggc tatctccccg gcgacctcaa cgagaagctg gcgccgtgga tggcggcggt
      421 gcacgacaac ctctacgccc tcttctccga cgccggtgtc gacggggcga agcacgccat
      481 cgaggagctg gtggagcggg gcgaattgga gatggcggcg gtcacctatc tgcggggccg
      541 gtcgatcacc ggtgagttcg tcatcatcga cgaggcgcag aatctggagc tgcccacgtt
      601 gaaggtcatc ctgacccgga tgtcccggga ttcgaaggtg gtcttctgtg gtgatctgac
      661 ccaggtcgac aacccctata tctccccgtt tggcgggctc gccgcgttga tcgagaagtt
      721 caagggctcg tcactgtttg gccacgtgac cctggagaag tcggtgcgtt ccccgctggc
      781 cgagatggcg gcaacgatcc tctagttgcg acccgaaccg gtttgccgtc ccgcggctcc
      841 ggctgagacg agctctccca cacccggcct gggaacgggg atggtgaact cgaaacatgc
      901 tccgccgagt cgctctgagg tccggtattc gatggtcccg ccgtgggcgg agaccagatc
      961 tcttgccacc gagagaccga tgcccgagcc cggcacgctg gtgttctgct ggtggagacc
     1021 ccgctcgaac ctctcccaca tctcggattg atgcttggcc ggcaccccgt cgccgttgtc
     1081 atgaaccgcg aacaacacca ccgggccact ccgggcgact gtcaccagga tctgatcccg
     1141 gccgtagcgc accgcattcg tgagcagatt gaccaggacc tggcggatgc gatcgggctc
     1201 ggcccacacc ctggcctcgg catcggcctc gacccggatc cccttgccac caccgccgac
     1261 cgccaccgct tcgcgggcga ggtctgccgc gttgtagtcg accagcttca gctccacgtt
     1321 ttgcaggcgg tcccgggcga catcgatgag atcggtgacg atgtggccga ggtggttggc
     1381 ctgcgtgttg atcgtctcca gcatctcgtc gcgttcttcc gggctgagct cttcccagga
     1441 ctcgccgagg atctgggcat aaccctggat cccggtgagc ggtgtcctca actcgtggga
     1501 gaccgaggcg acgaggtcgt ctcgctgttg ctcgagcagc tccctctttt cgcgtaccac
     1561 aaggttctga cgcaccacca ccatcaccgc cacggcggtg gtggtgatg
//
LOCUS       DATITW010000017         1556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_561815, whole genome shotgun sequence.
ACCESSION   DATITW010000017 DATITW010000000
VERSION     DATITW010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561815"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VLS86_00180"
     CDS             <1..333
                     /locus_tag="VLS86_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSO48933.1"
                     /translation="GDNIGIEVSGGDGVVDIEVWDDGPGVPEEQVEKLFERFIHDGSA
                     PLLTGSIGLGLAVASRLTALLGGKLSYQRFVGKTYFIVSLPGLEQVAEETGGSQESVA
                     EMIRAMSA"
     gene            330..1436
                     /locus_tag="VLS86_00185"
     CDS             330..1436
                     /locus_tag="VLS86_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO48934.1"
                     /translation="MKRVLGLVLIVMILALALPVAATENANYKDKFDSIGWGGSNGSL
                     PWSGPWSEIGDDNDEKYGSVRVVSSGNCPSGGVCMRISATLILTDIGARRGADLSVLQ
                     EPELCFDVVNIPDALSLPGTKLRVQVNTGSGWSTLDGGEWDLGDELEAHPTFDLSDFR
                     AENFQVRFLVVGLLSTSEVFIDNVEITGELIETTTTTTVPDTTTSTQGDDGPATTTTT
                     TKPDAPTTTTTIRATTTTRPSNTPGGAVDDITTSTTVASTTTTTEAGDGNDAILIGGG
                     GGSDDGEPPGLDDSPAGSGIRQTARGLQANFDAGLYGDVSAISPITGVDYQARFSMTV
                     EIIEATWAWIVLLALVIAWSIVSGMDRRKSQPLG"
BASE COUNT          301 a          484 c          506 g          265 t
ORIGIN      
        1 ggcgacaaca tcgggatcga ggtctccggt ggtgacgggg tagtcgacat tgaggtctgg
       61 gatgacgggc ctggggttcc cgaggagcag gtggagaagc tcttcgagcg cttcatccac
      121 gatggctccg cacccttgct cacggggagc atcgggctcg gcctcgccgt ggcctcgcgg
      181 ctgaccgcac tcttgggcgg aaagctttcc tatcagcgat tcgtaggcaa gacctacttc
      241 atagtgagtc ttcccggtct ggaacaagtt gccgaagaga cgggcggcag ccaggagtcg
      301 gtcgccgaga tgatccgggc gatgtcggcg tgaaacgggt cctgggcctg gtgctgatcg
      361 tcatgatcct ggctctggcg ctgccggtag cggcgaccga gaacgccaat tacaaggaca
      421 agttcgactc catcgggtgg ggcgggtcaa atggctcact tccatggtcc gggccgtgga
      481 gcgagatcgg cgacgacaac gacgagaagt acggctcggt ccgggtggtg tcctcgggga
      541 actgcccatc gggcggggtt tgcatgcgga tcagtgccac tctgatcctg accgacatcg
      601 gggccagacg aggagccgac ctcagcgtcc tccaagagcc ggagctctgt ttcgacgtgg
      661 tcaacatccc ggacgcgttg agtctcccgg gcaccaagct gcgggtccag gtgaacaccg
      721 gcagcggttg gtcgacactc gacggcgggg agtgggactt gggcgatgaa ctcgaggcgc
      781 atcctacctt cgacctctct gacttccgag ccgagaactt ccaggtccgt ttcctggtgg
      841 taggcctcct cagcaccagc gaggtgttca tcgacaacgt cgagatcacc ggggagttga
      901 tcgaaaccac gaccaccaca actgttcccg acaccaccac atccacacag ggcgatgacg
      961 ggccggcaac caccaccacg accaccaagc cggacgcgcc cacgaccacc accacgatcc
     1021 gtgcgacgac aaccacccgc ccgtcgaaca ccccgggcgg ggccgtcgac gacataacga
     1081 cctcgacgac tgtcgcttcc acgacaacca ccaccgaggc cggcgacggc aacgacgcga
     1141 tcttgatcgg tggtggcggg ggctccgacg atggggagcc tccgggtttg gatgactcgc
     1201 cggcgggaag tgggatcagg cagacggctc gcggtctgca ggccaacttt gacgccggcc
     1261 tctatggcga cgtgagcgcg atctcaccga tcacgggtgt cgactaccag gctcgattca
     1321 gcatgacagt cgagatcatc gaggcgactt gggcttggat cgtgctgctc gccctggtga
     1381 tcgcatggtc gatcgtgtcg ggcatggacc ggcggaagag ccaaccgctc ggctgacaag
     1441 cgcctacgcg agatccattg tctcctcatt tgaggtgaag atcaccagca ggccgaccag
     1501 gccgtgcact gccacgacta ccgcgaccat caacggtgcg tattccggat ccgagt
//
LOCUS       DATITW010000018         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_241276, whole genome shotgun sequence.
ACCESSION   DATITW010000018 DATITW010000000
VERSION     DATITW010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241276"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..803)
                     /locus_tag="VLS86_00190"
     CDS             complement(60..803)
                     /locus_tag="VLS86_00190"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HSO48935.1"
                     /translation="MSLRTRRSTFAIIGGLAVVLAACGGTTPQAITVNNSEESQMGIS
                     VTGMGEVTGTPDTVEVDLGVSILAPTVAEATSLAAEKADSLIAALTSNGVAREDIATT
                     DYSIYPEYDYSTNAQRLLGYRVSTTVRAKIRNVQETGAVLDSVTTAAGDEVRISGLRF
                     SIEDNAELITAAREAAWNDAFDKATELAALSGQTLGAATSISETVSNPPVPIPYAADE
                     AGAERAVTPIEPGTSAVTITLQVEFAFEG"
     gene            complement(828..1106)
                     /locus_tag="VLS86_00195"
     CDS             complement(828..1106)
                     /locus_tag="VLS86_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HSO48936.1"
                     /translation="MTAFEDAVIEVLASLEAGDVVTYGEVAAQAGFPGAARAVGTILR
                     TSDLDLPWWRVVGAGGLIRTPQPERQAELLAREGILVAGGKVVGVRNR"
     gene            complement(1103..1723)
                     /gene="upp"
                     /locus_tag="VLS86_00200"
     CDS             complement(1103..1723)
                     /gene="upp"
                     /locus_tag="VLS86_00200"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HSO48937.1"
                     /translation="MPLTILDHPLAADLLAILRNKDTEPPEFRQIAERLGYLLVAEAL
                     SDMSTVDIEIETPLEKTSGQQLRRPVVAVAVLRAGLGLLHSVLTLVPDAAIGFAGVQR
                     NEETAEPIEYYTKFPELSSARVLILEPMLATGGSLSWAIDKVKENGAKDITAVCVVTA
                     PEGLARIEREHPDVRIVAAASDRELNPQFYIAPGLGDMGDRLFGTL"
     gene            complement(1749..>2119)
                     /locus_tag="VLS86_00205"
     CDS             complement(1749..>2119)
                     /locus_tag="VLS86_00205"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006143823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HSO48938.1"
                     /translation="WGFFQTFLSNVEMTLAKTDLDIAGRYVTSLVPPDHQGLLDLVRA
                     EYELTTEEILTLRKEPVLLDDLPLLKRTLGVRDIYLDPINYLQVSLLARSRAGESGEE
                     LNRALLLTVNGIAAGMRNTG"
BASE COUNT          344 a          674 c          710 g          391 t
ORIGIN      
        1 ggcgcgatat tcctctctcc gtcgcacaaa gccctcgccg gcgccgtgga cagggaccgc
       61 tagccctcga aggcgaactc gacctggagg gtgatggtca ccgccgacgt gcccggctcg
      121 atcggggtca cggctcgctc cgcgcctgct tcgtcagctg cataggggat gggaaccggt
      181 gggttgctca ctgtctccga gatcgacgtg gcggctccaa gggtctgtcc cgacaatgcg
      241 gccagctcgg tcgccttgtc gaatgcgtcg ttccaggcgg cctcgcgggc ggccgtgatc
      301 agctctgcgt tgtcttcgat actgaaccgg agcccgctga tccggacttc gtcacctgcg
      361 gcggtggtga ccgagtcgag gaccgctccg gtctcctgga cattgcggat cttggcccgg
      421 accgtggtgc tcacccgata accgagcagt cgctgggcat tggtggagta gtcgtactcg
      481 gggtagatcg agtagtcggt ggttgcgatg tcctcgcggg cgaccccgtt ggaagtcagg
      541 gcggcaatca gtgagtcggc cttctccgcg gcgagcgacg tcgcctcggc gacagtgggg
      601 gcaagtatcg aaacgccgag atcgacttca accgtgtccg gggtgccggt cacctctccc
      661 atcccggtta ccgagattcc catctgtgat tcctccgagt tgttcaccgt tatcgcctgc
      721 ggtgtggtcc cgccacaggc ggcgaggacg acggccaggc cgccgatgat tgcgaatgtc
      781 gaccgtctgg ttctcaggct catgtccttt ggacggtcgt gggcggctca acggttcctg
      841 acccctacca cctttccccc ggcgacgagg atcccctccc tcgccagcag ctcagcctgt
      901 ctctccggct ggggagtcct gatcaggccg cccgccccga ccacacgcca ccagggaaga
      961 tcgaggtcag aagtcctgag gatggtgccc acggcgcgag ccgcccccgg gaaccctgct
     1021 tgggccgcga cctcgccgta ggtcaccaca tcgccggcct ctagggaggc gaggacttcg
     1081 atgacggcgt cctcgaaggc ggtcaaagcg tgccgaagag cctgtctccc atgtctccca
     1141 gacccggcgc gatgtagaac tggggattga gctcccggtc ggacgcggcg gccacgattc
     1201 gcacgtcggg atgctcgcgc tcgatgcggg ccagaccctc gggcgcggtg accacacaca
     1261 cggcagtgat gtcctttgcc ccgttctcct tgaccttgtc gatcgcccag gagagcgagc
     1321 ctccggttgc cagcatcggc tccaggatca acacccgcgc tgaggagagc tcggggaact
     1381 tggtgtagta ctcgatgggc tcggcggtct cctcgtttcg ctggacgccg gcgaagccga
     1441 tggccgcgtc ggggaccagg gtgagcaccg aatgcaacag cccgagaccg gcccgcagca
     1501 cggccacggc gaccaccggc cgccgcaact gctggccgct cgttttctcc agcggggttt
     1561 cgatctcgat gtcgacagtg gacatgtcgg agagggcttc ggccacgagc agatagccga
     1621 gacgctcggc gatctgtcga aactcgggtg gctcggtgtc cttgttgcgg agaatggcga
     1681 gaagatcggc tgccagcggg tgatcgagga tggtgagggg catgggactc agcctaattt
     1741 gggccgactc agcctgtatt tcgcatcccg gcggcgatgc cgttgacggt gagcagaagg
     1801 gcgcggttca gctcctcccc cgactcgcct gcccgacttc gggcgagcaa cgagacctgg
     1861 agatagttga tcgggtccag gtagatgtcg cgcacgccca gggttcgctt gaggagcgga
     1921 aggtcgtcga ggagcaccgg ctccttgcgc agggtgagga tctcctccgt ggtcagctcg
     1981 tactcggctc gaaccaggtc gagaagaccc tgatgatccg gcggcaccag gctggtcacg
     2041 tatcgaccgg cgatgtcgag gtctgtcttg gccagggtca tctcgacgtt ggacagaaaa
     2101 gtctggaaga agccccact
//
LOCUS       DATITW010000019         1614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_601877, whole genome shotgun sequence.
ACCESSION   DATITW010000019 DATITW010000000
VERSION     DATITW010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601877"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VLS86_00210"
     CDS             complement(<1..224)
                     /locus_tag="VLS86_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016814838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSO48939.1"
                     /translation="MADSNASSLNAQRRRADLARMREQPFDLAVIGGGVTGCGVALDA
                     ASRGLSVALVEQRDFASGTSSRSSKLFHGGL"
     gene            258..1172
                     /locus_tag="VLS86_00215"
     CDS             258..1172
                     /locus_tag="VLS86_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSO48940.1"
                     /translation="MTGQMKIGVQLPEVEREVRWPEIRDIAHTAEEVGFDSLWLGDHL
                     LYNDPVAGARGPWEAWSLLAALGEATSRVMIGPLVAATSFHSPAMLAKKAATVDEISG
                     GRLILGLGAGWNRTEYDAFGFAYDHRVSRFEEAFSIIKTLIREGSIDHVGRFYTHRGM
                     ELLPRARRDIQLMVGSNGPRMLRITAPDVDMWNTWFTDFENRAENLAPLVAAVDSACR
                     DVGRDPGSVQRSAAVLVQVEGGSGRVAGSGERPVVTPLTGSKTELAERLAGFAGAGMD
                     HIQLVLDPIEARTVEEMGEVLRMLRRPG"
     gene            1236..>1614
                     /locus_tag="VLS86_00220"
     CDS             1236..>1614
                     /locus_tag="VLS86_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO48941.1"
                     /translation="MANLRITRLGHGGVLYRSPNDAWIWVDRWTGAPNHAAAFRTPEK
                     VSVVAPTHCHFDHVGDDCVDLVELAEVEDATVIGSHELSVWLGNRGTDSIGMNKGGTV
                     EAAGVRFTMVHADHTGGATLGTGS"
BASE COUNT          293 a          518 c          548 g          255 t
ORIGIN      
        1 agaccaccgt gaaacagctt gctggagcgg ctcgaagtcc ccgaggcgaa gtcccgctgc
       61 tccaccaggg ccaccgacag ccctcgtgac gccgcatcga gggcgacgcc acatcccgtc
      121 accccacctc cgatcacagc gagatcgaag ggctgctctc gcatccgagc caggtcggcg
      181 cgtcgccgct gtgcgttgag actggatgca ttcgagtcgg ccaatgtccg cagaggtcta
      241 gccgtaccat ctcgcgaatg acaggtcaga tgaagatcgg tgttcagctc cccgaggtgg
      301 agcgagaggt gagatggccc gagattcggg acatcgcgca cacggcggaa gaggttggat
      361 tcgattcgct ctggctcggc gatcatctcc tctacaacga tccggtggcc ggtgcccgcg
      421 gcccgtggga ggcatggtcc ctgctcgcgg ccctcggcga agcgacgagc cgggtgatga
      481 tcggcccgct ggtggccgcg acctcgttcc acagcccggc gatgctggcc aagaaggcgg
      541 ccaccgtcga cgagatcagt ggagggcggc tgatcctcgg cctgggagca gggtggaaca
      601 ggaccgagta cgacgccttt ggattcgcct acgaccatcg cgtctctcgc ttcgaggagg
      661 cgttctccat aatcaagacc ctgatccgcg aaggatccat cgaccacgtc ggtcgcttct
      721 acacccatcg cggaatggag ctcctgcccc gcgcccggag ggacattcag ctcatggtcg
      781 gctccaacgg gccacggatg ttgaggatca ccgcccccga cgtcgacatg tggaacacgt
      841 ggttcaccga cttcgagaat cgcgccgaga acctggcccc gctggtcgcc gccgtggaca
      901 gtgcctgtcg cgacgttggc cgtgatccgg gaagcgtgca gcgctccgca gcggtgctcg
      961 tccaggtcga aggaggttcg ggccgcgtgg ccggaagtgg cgagcgaccc gtggtgaccc
     1021 cgctcaccgg ctcgaagacg gagctggccg agagactggc gggattcgcc ggtgcgggca
     1081 tggatcacat ccagctggtg ctcgacccga tcgaggcccg cacggtggag gagatgggcg
     1141 aggtcctgag aatgctgcga cgaccgggtt gaccacgacg tcggattcgg gcgccgcata
     1201 ggatcgaaga ccgactcgag gaggcaccag acacgatggc aaaccttcgc atcacccggc
     1261 tggggcacgg tggcgtgctc tatcgatctc cgaacgacgc atggatctgg gtcgaccgat
     1321 ggaccggcgc cccaaatcac gctgctgcct ttcgcacccc ggagaaagtg agcgttgtcg
     1381 cccccaccca ctgtcacttc gaccacgtgg gagacgactg tgtcgacctg gtggagctcg
     1441 ccgaggtcga agatgcgacc gtgatcggct cccacgagct gagcgtgtgg ctcggcaacc
     1501 ggggcaccga ttcgatcggg atgaacaagg ggggaaccgt cgaggcggcc ggggtccggt
     1561 tcacgatggt ccatgcggac cacaccggtg gcgccacgct cggcaccggc agcg
//
LOCUS       DATITW010000020         1570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_121233, whole genome shotgun sequence.
ACCESSION   DATITW010000020 DATITW010000000
VERSION     DATITW010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121233"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VLS86_00225"
     CDS             complement(<1..602)
                     /locus_tag="VLS86_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO48942.1"
                     /translation="MKRLTRTIAALIVMALAIPALGQVTDEDIDRARREVNEILADSE
                     ALGIEVQEAWARQFSLEKEISDLNSSIEVAKVKITETERKLEEVAVEMYMGSTSVATI
                     QVLFSTSDDAYGAGFEYLRNVSGVEGDVVNQLRSFRTELGRQTERLAEASVEQQALAA
                     ELEVKATELQGDLVAAQGVYEGLVQQQAAEEEARRRAEEEA"
     gene            complement(599..1375)
                     /locus_tag="VLS86_00230"
     CDS             complement(599..1375)
                     /locus_tag="VLS86_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSO48943.1"
                     /translation="MQVERILANNPGPFTGPGTNTWLLDDGAGTVVIIDPGPIDDTHL
                     ERVLERLVGRQAIGVLVTHTHPDHAPLANPMARELQVPAYGHSPGPEFDPDLLLADGS
                     SLDVGKLRLVVIHTPGHADDHLCFRVGDVLFTGDHIMGGSSVMVEDMGRYLDSLTRLR
                     GTGLRRLHPGHGDDMDDPDQVIDWYLAHRRQRHEQIFEAIAAGARSASEVLELVYADV
                     DRSLYPLAARSVEAHLTLLRDQGRIALSPEREISVPPEPS"
     gene            complement(1375..>1570)
                     /locus_tag="VLS86_00235"
     CDS             complement(1375..>1570)
                     /locus_tag="VLS86_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO48944.1"
                     /translation="SSALHRHEQGELEMFLPTIAHLRWLERRSTVEDAIASASGADGR
                     SLIQPQRMEDGSMLPIHMPS"
BASE COUNT          264 a          525 c          510 g          271 t
ORIGIN      
        1 gcctcctcct cggcgcgccg ccgagcttcc tcttcggctg cctgctgctg aaccaggccc
       61 tcatagacgc cctgtgcggc gaccaggtca ccttgcagct cggtcgcctt cacctcgagc
      121 tcggcggcga gcgcctgctg ctcgaccgac gcctcggcca gccgttcggt ctgtcggcca
      181 agctcggtgc ggaatgagcg aagctgattg accacatcgc cctcgacccc ggagacgttc
      241 ctcagatact cgaagccggc gccataggcg tcgtcactgg tcgagaacag aacctggatg
      301 gtcgccaccg acgtcgagcc catgtacatc tcgaccgcca cttcttccag cttgcgctcg
      361 gtttcggtga tcttgacctt ggcgacctcg atcgacgagt tgagatcgga gatctccttc
      421 tccagggaga actggcgggc ccaggcctcc tggacctcga tgccgagcgc ctccgagtcg
      481 gccaggatct cgttgacctc ccgtcgggcg cgatcgatgt cctcgtcggt gacctggcca
      541 agggcaggta tggccagtgc catgacgatc agggcagcga tggttctggt gagacgcttc
      601 acgagggctc ggggggaacg gagatttccc gctccgggga caaggctata cggccctgat
      661 ccctgagcaa cgtgaggtgg gcctccaccg agcgggcggc gagcgggtac agcgagcgat
      721 ccacgtcggc gtacaccagc tccaggacct ccgatgcgga ccgggcaccg gccgcgatcg
      781 cctcaaagat ctgctcgtga cgctgacgtc gatgggcgag ataccaatcg atcacctggt
      841 ccgggtcgtc catgtcgtcg ccatgaccgg gatggagccg cctcagcccg gtcccccgga
      901 gacgggtcaa tgagtccaga tagcgaccca tgtcctcgac catcaccgac gagccgccca
      961 tgatgtgatc tccggtgaac aggacatcac ccacgcgaaa gcagagatgg tcgtccgcat
     1021 ggcccggggt atggatcacc accagccgga gcttcccgac atcgaggctc gagccgtcgg
     1081 ccagcagcag gtcgggatcg aactcagggc ccggggagtg cccgtaggcg ggcacctgca
     1141 gctcgcgcgc catcgggttg gcgagcgggg catgatccgg atgggtatgg gtgaccagca
     1201 ccccaatcgc ctgtctcccg acaagcctct ccagcacgcg ctcgaggtgg gtgtcgtcga
     1261 tgggccccgg atcgatgatc accacggtcc cggccccgtc gtcgaggagc caggtgttgg
     1321 tgccgggccc ggtgaacggc cccgggttgt tggccagaat ccgctcgacc tgcatcagga
     1381 cggcatgtgg atcgggagca tcgatccgtc ttccatcctc tgcggctgga tcagggagcg
     1441 gccatcggcc ccgctcgccg acgcaatcgc gtcttcgacc gtggatcgtc gctccagcca
     1501 acggaggtgg gcgatcgtgg ggagaaacat ctccagttcg ccttgctcat gtcggtgcag
     1561 cgcggacgac
//
LOCUS       DATITW010000021         1532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_361654, whole genome shotgun sequence.
ACCESSION   DATITW010000021 DATITW010000000
VERSION     DATITW010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361654"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1532
                     /locus_tag="VLS86_00240"
     CDS             <1..>1532
                     /locus_tag="VLS86_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HSO48945.1"
                     /translation="GSIILWGLVLAVFTWAVARRALDRLGAAALAVMGGVGVFFFGLM
                     LTVANPFEVCVDALATGCAASSAWPFTAADIPLNGPGPNPLLQNHFLMAVHPPVLYIG
                     YVGLTVPFAYAIGALALALPGPEWLRRSHRSTLVAWSFLTLGITLGGWWAYEVLSWGG
                     YWAWDPVENASLMPWLAATAFIHSGLVQQRRGMLQAWNFVLVISAFALTILGTFLTRS
                     GTINSVHSFTQSAIGPALLGFLVLVLVGSFTLFAMRSHLVASSPRIETFVSREGTFLV
                     NNLFLTVYAFVVLIGTTYPLLLEAFTGTQVGVGEPFFNRLAVPLSFMLLLTMGFGPVT
                     PWRGAAPGLVWRRLRWPTILALAVGLIVALTVTRVAWVILAIVLGVFVTAAIGCLLVE
                     LARNRKAKSGRPLYQEAKTVVGNDQAFWAGQISHIGVVLVAIGIAFAANLPLHDEVEL
                     APGESVDFAGYTLTYDAPFRREEPNRVAQGVRVHVSQGDRLVATLEPRANFYDSAMGG
                     II"
BASE COUNT          198 a          527 c          487 g          320 t
ORIGIN      
        1 agggatcgat aatcctctgg ggcctcgtcc tggccgtgtt cacctgggcg gtggcccgac
       61 gggcgctgga ccgcctcggg gcggccgcgc tggccgtgat gggcggggtg ggcgtgttct
      121 tcttcggtct gatgctcacc gtggccaatc ccttcgaggt gtgcgtcgat gctctggcca
      181 cggggtgcgc cgcctccagt gcgtggcctt tcaccgccgc cgacatcccc ctcaacggcc
      241 cgggccccaa cccattgctg cagaaccatt tcctgatggc ggttcacccg cctgtcctct
      301 acatcggcta tgtcgggctg accgtgccct ttgcatacgc gatcggagcc ctggccctcg
      361 cacttcccgg gcccgagtgg ctgcgtcgga gccatcgctc gaccctggtc gcctggtcgt
      421 tcctcaccct cgggatcacc ctcggaggct ggtgggccta cgaggtcctt tcgtgggggg
      481 gctactgggc ctgggatccg gtggagaacg catcgctgat gccgtggctc gctgccaccg
      541 ccttcatcca ctccggattg gtccaacagc gccgggggat gctccaggcg tggaacttcg
      601 tcctggtgat cagcgccttc gccctcacca tcctcgggac gttcctgacc cggtcgggga
      661 ccatcaactc ggtccactca ttcacgcaat cggccatcgg gccggcgttg ctcggattcc
      721 tggtgttggt gctagtgggc tcattcactc tcttcgcgat gcgctcgcac ctggtcgcct
      781 cctcaccacg catcgagacc ttcgtgtccc gagagggcac cttcttggtc aacaacctct
      841 tcctcaccgt ctacgccttc gtggtcctga tcgggaccac ctatcccctg ctcctggaag
      901 ccttcaccgg gacccaggtc ggggtgggtg agcctttctt caaccggctg gccgtgcccc
      961 tctccttcat gctgctgctg acgatgggtt tcggtccggt gacgccgtgg cgtggggctg
     1021 cgcccggcct ggtctggcga cgcctccgct ggccgaccat cctcgccctc gcagttggtc
     1081 tgatcgtggc tctaaccgtg acccgggttg cctgggtgat cctcgccatc gtcctcggcg
     1141 tcttcgtgac agctgcaatc gggtgtctcc tggtggagct ggcgcggaac cgaaaggcca
     1201 agtcgggacg gccgctctat caggaggcga agaccgtggt cggcaacgac caggcattct
     1261 gggcgggcca gatcagccat atcggggttg tgctcgtcgc gatcggcatc gcctttgccg
     1321 ccaatctccc cctccacgac gaggtcgagc tggccccggg cgaatcggtt gacttcgccg
     1381 gttacaccct gacctacgac gcgcccttcc gacgggagga gccgaatcgt gtggcccagg
     1441 gggtgcgggt ccatgtcagt cagggtgacc gcctcgtggc cacgctcgaa ccgagggcga
     1501 acttctacga cagcgcgatg ggagggatca tc
//
LOCUS       DATITW010000022         1771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_281536, whole genome shotgun sequence.
ACCESSION   DATITW010000022 DATITW010000000
VERSION     DATITW010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281536"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1752)
                     /locus_tag="VLS86_00245"
     CDS             complement(<1..1752)
                     /locus_tag="VLS86_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSO48946.1"
                     /translation="MTAGVAAPVLDLYGRNPSVFVANRSTPEQIVMFGLLVTFTLPLI
                     ALVILTVTEKISPRAADIAYYILVGVSAAATGLVISRQVVPESTLGAIGLAIAVTVVV
                     LLLYRRLEPGLLFFSLLGPAVLVMFLAISPTSRLLAEPESPPVTPDQVGLAAPIVFIQ
                     LDELPMASLMREDGTINVALFPNFARLAASGNWYRNALSNSIATTSSVPAILTGRLGD
                     KEKSPSLIDHPNNLFTLLGEGYEMHVVEWLTDMCPEEVCKDYAGRGPARFGSLLQDVG
                     VVYGHLSLPESARAGLPSIDGSWKGFLGQAEAREAAPVDVGDLPVPEPGVRSKWIDWI
                     ERIIDGVEAGAAPTLHFAHLQAPHIPWRINPSGSHYERPEQYDEVDGIEGGKWVTRDD
                     LPRLGYQRHLYQLGFLDQRLGALFDRLEETGTWDDALIVVVADHGASFVAGEHRRWPM
                     EGNLSDLYRVPLIIKLPGQVEGQVFDEPAFAIDIVPTIVDALDITTEWEFDGHSLLEI
                     EGTDYPHQPIFYCCSPDPASTDIAELFEQVRRNHQWVPDQGSWMAIAGSGLYADLVGQ
                     PVASLDPQPSGALAWSLT"
BASE COUNT          333 a          558 c          587 g          293 t
ORIGIN      
        1 ggtcaacgac caggcgaggg caccgctcgg ttgcggatcc aggctggcca ccggctggcc
       61 gacgaggtcg gcatagaggc cagatccggc gatagccatc caactcccct gatcgggcac
      121 ccattggtga ttgcgccgca cctgttcgaa cagctcggcg atgtcggtgc tggccgggtc
      181 cgggctgcag cagtagaaga tcggctggtg tgggtaatcg gtcccttcga tttccaggag
      241 ggagtggccg tcgaattccc actcggtagt gatatccagg gcatcgacga tggtgggaac
      301 gatgtcgatg gcgaacgccg gctcgtcgaa gacctgaccc tcgacctgtc ccgggagctt
      361 gatgatgagc ggaacccggt agagatcgct gagatttccc tccatcggcc agcgtcggtg
      421 ctctcccgcg acaaacgagg cgccgtggtc ggccacgacc acgatcagcg cgtcgtccca
      481 ggtgccggtc tcctccagac ggtcgaacaa cgcccccaga cgctgatcca ggaagccgag
      541 ctggtacagg tgacgctggt accccaatcg ggggagatcg tccctggtca cccatttgcc
      601 gccttcgatg ccgtcgacct cgtcgtactg ctcgggtcgc tcgtagtggc tccccgatgg
      661 gttgattcgc catgggatat gaggcgcctg gagatgagcg aagtgcaggg tgggtgccgc
      721 accggcctcg accccgtcga tgatccgctc gatccagtcg atccacttgc tcctcacccc
      781 cggctcggga accgggaggt cgcccacatc gacgggcgcc gcctcccgcg cctccgcctg
      841 tcccagaaag cccttccaac tgccgtcgat gctggggagc ccggcacgcg cagattcggg
      901 aagagagagg tggccgtaaa ccacccccac gtcctggagc aaggaaccga agcgggccgg
      961 ccctcggccg gcgtagtcct tgcacacctc ctcggggcac atatcggtga gccactcgac
     1021 gacgtgcatc tcgtatccct cgccgagaag cgtgaacaag ttgttggggt gatcgatcag
     1081 cgaagggctc ttctccttgt cgccgagacg cccggtgagg atggcgggga cactggatgt
     1141 ggtggcgatc gagttggaga gcgcattgcg ataccagttc ccggaggcgg ccaatcgagc
     1201 gaagttgggg aatagggcga cgttgatggt cccgtcctct cgcatcaggg aggccatcgg
     1261 cagctcgtcg agttggatga acactatcgg ggctgccaat ccgacttgat cgggggtgac
     1321 gggtggcgat tcgggctcgg ccaagagacg tgacgtgggc gatatcgcca ggaacatcac
     1381 cagcaccgcc ggcccgagca gcgagaagaa caagaggccg ggctcgagcc gccgatagag
     1441 cagcaacacg acgaccgtca cggcgatcgc cagcccgatg gcccccagcg tgctctcggg
     1501 caccacctgg cggctgatga ccaggccggt tgccgccgcc gagaccccca ccaatatgta
     1561 ataggcgata tcagcggccc gggggctgat cttctcggtg acggtgagga tgactaacgc
     1621 aatcagcggc agcgtgaatg tgaccagaag cccgaacatg acgatctgct ccggcgtgct
     1681 ccggttggcg acgaagacgc tggggttcct gccgtacagg tccagcaccg gagcggccac
     1741 accagcggtc atcaggccga ggatcaccaa c
//
LOCUS       DATITW010000023         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_682262, whole genome shotgun sequence.
ACCESSION   DATITW010000023 DATITW010000000
VERSION     DATITW010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682262"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="VLS86_00250"
     CDS             complement(<1..1409)
                     /locus_tag="VLS86_00250"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HSO48947.1"
                     /translation="MTANPDEALRADIRRLGNQLGDALVRQHGPGLLELVEQVRALGK
                     SSRRGGSTEAAGQLDQLLAGLTITDVIPLVRAFTAYFYLANVAEQVHRIDQLSIDELG
                     LAATVDRVLAADVDPELLTSVLKRLEVRPVFTAHPTEAARRSILSKTVVLAELIERRL
                     HATDRERDRIDRRTAETIDEIWQTDELRLQKPNPVEEARSALYYLVQIAAEVMPDLSE
                     SVAVELARLGTEITASPIRFGSWVGGDRDGNPGVTPETTLETLLLHHDRGLRHLIGLI
                     EELSEELSVSELLAPISDELRESLERDREALPEVWRRERRRTASEPYRLKCSYIHARL
                     LNTSRRLRQGLAHVTGHDYPHPSALVADLDLMADSLVRSKGELIAQGAVARVRRAIET
                     FGFQVATLDVREHSSKHRETITELFSLVDVDYQSLDPEQRAAVLASELDGARPLSGIT
                     TEFTEESARTLGTFHAIREA"
     gene            complement(1439..>2140)
                     /locus_tag="VLS86_00255"
     CDS             complement(1439..>2140)
                     /locus_tag="VLS86_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HSO48948.1"
                     /translation="EGPEVGSKVVLDSTGTILSGELPAEIAAEVTADALVLMDHEQHR
                     SMGYPPHDVFVETLAPPQEMLIFGAVHIGQSLASFATQMGYRVTVADSRAAFATPERY
                     PTARVLVGWPEDVLDQVRLDRRTWVIILSHDKRHEDPVLEAALKSPVRYIGAMGSRRT
                     QDLRVERLSEKGFSGEDIARVRGPVGLDIGAESPQEVAVSILAEITMVRYGHGTGLSL
                     HGTEGRVHKQRPPDA"
BASE COUNT          327 a          721 c          714 g          378 t
ORIGIN      
        1 gcctcacgaa tcgcatgaaa ggtgccgagg gtgcgggcgg attcctcggt gaactcggtc
       61 gtgatcccgc tcagcggtcg cgctccgtcc aactctgagg cgaggacggc tgccctctgt
      121 tccgggtcca ggctctgata gtcgacatcg acgagcgaga acagctcggt gatcgtctcc
      181 cggtgcttgg aggagtgctc ccggacgtcg agcgtcgcca cctggaatcc aaaggtctcg
      241 atcgcccgtc gcacccgcgc caccgctccc tgggcgatca gctctccttt ggagcgcacc
      301 agcgaatcgg ccatcaggtc gaggtcagcc accagggcgc tcgggtgggg atagtcgtgc
      361 ccggtcacgt gtgccaatcc ctgccgcagc cgtcgactgg tgttgaggag acgggcgtga
      421 atgtatgagc atttcagccg atagggctcc gaggccgtgc gtcggcgctc acgccgccac
      481 acctcgggga gtgcctcccg gtcgcgttcc agtgactccc tcaactcgtc actgatcggg
      541 gcgagcaatt cggacacgga gagctcctcg gacagctcct cgatcaaccc gatcaggtgc
      601 cggagaccgc ggtcgtgatg gagcaggagg gtctccagcg tcgtctccgg cgtcacccct
      661 ggattcccgt cccggtcccc gccgacccac gagccaaacc ggatcgggct cgctgtgatc
      721 tctgtcccca gcctggccag ctccaccgca acgctctcgc tgagatctgg catcacctcg
      781 gcggcgatct ggaccaggta gtagagggcg gagcgggctt cctccaccgg gttcggcttc
      841 tgcagcctca gctcgtcggt ctgccagatc tcgtcgatgg tctccgcggt tcgtcgatcg
      901 atgcggtccc gctcccggtc agtggcgtgg agccgtcgct cgatcagctc ggccagcacc
      961 accgtcttgc tcaggatcga gcggcgggca gcctccgtgg gatgagcggt gaacacaggt
     1021 ctgacctcga gccgcttcag gacggaagtc agcagctcgg gatcgacatc tgcggccaaa
     1081 acccgatcga cggtggcggc cagacccagc tcgtcgatgc tgagctggtc gatccgatgc
     1141 acctgctccg ccacgttggc caggtagaag tacgcggtga acgcccggac cagcgggatc
     1201 acgtcggtga tggtcagccc ggcgaggagc tggtcgagct gtccggcggc ctcggtgctc
     1261 ccaccacgtc gtgacgactt gcccaacgcc cgcacctgtt ccaccagctc gagcagcccg
     1321 gggccgtgtt gtcggaccag ggcgtcgccg agctggttgc cgagtcgccg aatgtcggct
     1381 cggagcgctt cgtccgggtt tgctgtcacc agcacaggct attcaggtgt tggacgactc
     1441 aggcgtcggg agggcgctgc ttgtgcaccc ttccctcggt gccgtgcagt gagagaccgg
     1501 tgccgtgtcc gtagcggacc atggtgatct cggccaggat ggacacggcc acctcctggg
     1561 ggctctcggc accgatgtcg agccccaccg ggccccgcac cctggcgatg tcctcgcctg
     1621 agaagccctt ctcgctgaga cgctcgaccc tcaggtcctg ggtgcgccgg ctgcccatgg
     1681 ccccgatgta acgcaccggg ctcttcaggg cagcctcgag caccgggtct tcgtgccgct
     1741 tgtcatggga gaggatgatg acccaggtcc ggcgatcgag gcggacctgg tcgagcacgt
     1801 cctcgggcca gcccaccagc acccgggccg tggggtagcg ctcgggggtg gcgaatgctg
     1861 cgcgggaatc ggccaccgtc acccggtatc ccatctgcgt ggcgaagctg gccagggact
     1921 ggccgatgtg gaccgcacca aagatcaaca tctcctgggg tggggccagg gtctcgacga
     1981 acacgtcgtg gggcgggtaa cccatcgacc ggtgttgctc gtgatccatg aggaccaggg
     2041 cgtctgcggt cacctccgcc gctatctccg ccggcagctc tccgctgagg atggtgccgg
     2101 tcgagtccag cacgaccttg gagccgacct cggggccttc
//
LOCUS       DATITW010000024         1647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_321739, whole genome shotgun sequence.
ACCESSION   DATITW010000024 DATITW010000000
VERSION     DATITW010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321739"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1647
                     /locus_tag="VLS86_00260"
     CDS             <1..>1647
                     /locus_tag="VLS86_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HSO48949.1"
                     /translation="CMGLLNTASSVRLNGTFAPVYPPAGNVAMSSQSGALGIAILDYA
                     TRNNIGISQFVSVGNKADVSGNDLMLAWEDDPEIDVITLYLESFGNPKKFYRIARRIG
                     RRKPIIAVKSGRSAAGSRAASSHTGALASSDVAVQALFRQAGVIRVDTIEELFDAANQ
                     LANQPIPAGERVGVVTNAGGPGILAADALEAKGLVLPELSAELRDKIGSRLPAEASTR
                     NPVDLIASGGPGEFEHATSLLLESGEVDAVMVIYVPVSPDGAPDVAAAINRSQRSYEG
                     EVPLLSVFMQTGDTAALLAGGDESRAIPTYMFPESAALALARAVRHGEWRQRDPGIEA
                     RIDHTVELELRALVDDALTRIPPEGGWLEPDMVDAILKVIGLRVPASSVVSSSKEAVA
                     AAAAFEGPAVLKVISASALHKSDVGGVVLGVRGDEAIKSAYKRVTGAVSDAEGVLVQE
                     YVEGGHEVLIGMTLDPNFGPLVVFGLGGIYVELLQDVTFRIHPLTDVDASEMIKEIKG
                     AKLLDGYRNQPHGDVAALEETLLQVSGLISAVPELVEMDLN"
BASE COUNT          272 a          532 c          557 g          286 t
ORIGIN      
        1 tgcatgggtc tgctcaacac tgcgtcgtcc gtccggctca atggcacgtt cgccccggtc
       61 tacccaccgg ccggcaacgt ggcgatgtcg agccagtcgg gcgccctggg gattgccatc
      121 ctcgactacg ccacccggaa caacatcggg atctcccagt tcgtctccgt cgggaacaaa
      181 gccgacgtca gcggcaatga cctgatgctc gcctgggagg acgatccgga gatcgacgtc
      241 atcaccctgt acctcgagtc gttcgggaac ccgaagaaat tctaccgcat cgcccgacgc
      301 attggtcgtc gcaagccgat catcgcggtc aagtcgggac ggtctgcggc tggcagtcgc
      361 gccgcgtcct cgcacaccgg cgcccttgcc tcgtcggatg tcgcggttca ggcgctgttc
      421 cggcaagccg gtgtcatccg ggtcgacacc atcgaggagc tgttcgatgc ggccaaccag
      481 ttggccaacc agccgatccc ggccggagag agggtggggg tggtgaccaa cgccggcggc
      541 cccgggatcc ttgccgccga cgccctcgag gccaagggcc tggtcctccc cgagctgtcc
      601 gccgagttgc gggacaagat cgggagcagg ctcccagccg aagcctcgac ccgcaacccg
      661 gtggatctca tcgcttcggg cgggcctggc gagttcgagc atgccacctc actcctgctg
      721 gagtccggcg aagtcgatgc ggtgatggtc atctacgtcc cggtgtcccc ggacggggct
      781 cccgacgtgg cggctgcgat caatcgatcg caacgttcgt acgaggggga ggtgcccctg
      841 ctctcggtgt tcatgcaaac cggcgacacc gccgcattgc tggccggcgg cgatgaatcg
      901 cgggcgattc ccacttacat gttccccgag tcggcggcgc tggccctggc ccgcgcggtt
      961 cgtcacggtg agtggcgcca gcgcgacccg ggcatcgagg ccaggatcga tcacaccgta
     1021 gagctggagc tgagggccct cgtcgacgac gccctgaccc ggatcccgcc cgagggaggc
     1081 tggctggagc cggacatggt cgatgccatt ctcaaggtca tcggcctgcg ggtgcccgcg
     1141 tcttcggtgg tgagctcgag caaggaggcg gtggcggcgg cagccgcgtt cgagggcccg
     1201 gcggtgctca aagtcatctc cgcatcggct cttcacaagt cagacgtcgg cggggtggtc
     1261 ctcggggtgc gaggtgacga agcgatcaag tcggcataca agagggtgac tggcgcggtc
     1321 agcgatgcgg aaggagtcct ggtccaggag tacgtagaag gcggtcacga ggtgttgatc
     1381 ggcatgactc tggatcccaa cttcggcccc ctcgtcgtct tcgggctggg tgggatctat
     1441 gtcgagctgc ttcaggacgt cacgtttcga atccacccgt tgaccgacgt cgacgcctcg
     1501 gagatgatca aggagatcaa gggcgccaag ctcctggatg ggtaccgcaa ccagccccat
     1561 ggcgatgtgg ctgccctcga ggagaccctg ctccaagtct cggggctgat cagcgccgtt
     1621 cccgagctcg tggagatgga cctgaac
//
LOCUS       DATITW010000025         1775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_201509, whole genome shotgun sequence.
ACCESSION   DATITW010000025 DATITW010000000
VERSION     DATITW010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201509"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VLS86_00265"
     CDS             <1..651
                     /locus_tag="VLS86_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSO48950.1"
                     /translation="YGLTKGQYSPTSEPGKTTKSSPYGSIDYPFNPVSLALGAEATFV
                     ARTLDMDRGHTQDLLQAAYDHEGSAFVEIYQNCNVFNDKAFIQLTGKEERVHNRIDLE
                     IGKPIVFDGGAKAIVLRDGQATIVDSSKVDPGAILVHDPTRPDPAVAFALSRLSHGPY
                     GPTPLGIFRNVHRPTYESQMNAQIEAAREKLGEGDLSRLISSQGTWTVKDPSSNGG"
     gene            complement(667..>1775)
                     /locus_tag="VLS86_00270"
     CDS             complement(667..>1775)
                     /locus_tag="VLS86_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSO48951.1"
                     /translation="HLGQEGRYGDGMAWNEAGKLNPGEWYCIEGEVALNRLGVADGAL
                     RAWVDGTPAFDEAGIEFRRPDEPNIKIESFWFNVYYGGKPRAERDFGLVIDEVVVDSA
                     RVGCGVGDSITRVVTADVTGDGHDDGLSWGPCPGGTCFSTGASTDPGRQVERSMGDGA
                     WFSLDGMRLGMAAGDFNGDGRDDVLYHGRCGRSVRCWRIHLSGGTALGTAQDWGDGAR
                     FAPTARSLISGDWNGDGFDDIVYQGLCGSGPVGCWRAHLNTDGLLVADDWGPLPGGAG
                     PVEMSSADLNGDGRDDLLYSSPCDTGDCWHGQMSTGESFSSPIDLGPSRPEAMVLNEL
                     FDTDGDGHADVVTVGEALDEFNLRHRAGVTGWPR"
BASE COUNT          304 a          646 c          556 g          269 t
ORIGIN      
        1 tacggcctga ccaagggcca gtactcaccg acctccgagc cggggaagac caccaagtcg
       61 tcgccatacg ggtcgatcga ctacccgttc aaccctgtgt ccctagccct gggggcggag
      121 gccaccttcg tcgcccggac gctggacatg gatcgcggtc acacccagga cctgctgcag
      181 gcggcgtacg accacgaggg ctcggctttc gtcgagatct accagaactg caacgtcttc
      241 aacgacaagg cgttcatcca gttgaccggc aaggaggagc gggtccacaa ccgcatcgac
      301 ctcgagatcg ggaagccgat cgtgttcgac ggtggggcca aggcgatcgt gctccgcgac
      361 ggccaggcca cgatcgtcga ctcgtccaag gtcgacccgg gagccatcct ggtccacgac
      421 ccgacgcgtc ccgacccggc ggtggccttc gccctgagca ggctgtccca tggcccgtac
      481 gggccgaccc cgctcgggat attccgcaac gtccatcgcc cgacctacga gtcgcagatg
      541 aacgcccaga tcgaggcggc ccgggagaaa ctgggcgaag gcgacctgtc ccggctgatc
      601 tccagccagg ggacgtggac ggtcaaggac ccctcgtcca acggaggctg accgagccga
      661 acctcgtcat cgaggccagc cggtgacccc cgcccggtgt ctcaggttga actcgtcgag
      721 cgcttcaccg actgtgacca cgtcggcgtg accgtcaccg tcggtgtcga acaactcgtt
      781 gagcaccatt gcctcgggtc ggctgggacc gaggtcgatg ggcgaggaga aagactcccc
      841 ggtggacatc tgtccgtgcc agcaatcccc ggtgtcgcac ggggacgagt agagaagatc
      901 gtcacggccg tcgccgttga gatccgccga cgacatctcc accggtcccg caccgccggg
      961 aagaggtccc cagtcgtcgg cgaccagaag accgtctgtg ttcaggtggg cgcgccagca
     1021 gccaaccggg cccgagccgc agaggccctg atagacgatg tcgtcgaagc cgtcgccgtt
     1081 ccagtccccc gagatcaggg agcgtgccgt cggggcgaac cgggctccgt caccccagtc
     1141 ctgggcagtg cccagcgcgg tgcccccgga gagatggatc cgccagcatc gcaccgagcg
     1201 cccgcacctc ccgtgataca agacgtcgtc acggccgtcg ccgttgaagt cgcccgcggc
     1261 catccccaac cgcatgccgt cgaggctgaa ccaggccccg tctcccatgc tcctctccac
     1321 ctgccgcccc gggtcggttg acgccccggt gctgaagcag gtgccaccag gacaaggccc
     1381 ccaggagagc ccgtcgtcgt gcccgtcccc ggtgacgtcg gcagtcacga cccgggtgat
     1441 gctgtccccg actccgcatc cgacccgggc gctgtcgacc accacctcgt cgatgaccag
     1501 gccgaagtcg cgttcggccc tgggcttgcc gccgtagtag acgttgaacc agaacgactc
     1561 gatcttgatg ttcggctcgt ccgggcggcg aaactcgatg ccggcctcgt cgaaggcggg
     1621 agtcccgtcc acccatgccc gcagcgcccc gtcggcgacg ccgagccggt tcagggccac
     1681 ttcgccctcg atgcagtacc actcgcccgg gttgagcttt cccgcctcgt tccaggccat
     1741 gccatccccg tagcgccctt cctggccgag gtgat
//
LOCUS       DATITW010000026         1508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_642406, whole genome shotgun sequence.
ACCESSION   DATITW010000026 DATITW010000000
VERSION     DATITW010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642406"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /gene="tatC"
                     /locus_tag="VLS86_00275"
     CDS             complement(<1..748)
                     /gene="tatC"
                     /locus_tag="VLS86_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HSO48952.1"
                     /translation="MRVIPRRRRSDEERSGTMTLLDHLGELRHRLIICLIALAVCAIP
                     GWFLYEPLKEALRAPYCDFVASNPSLVPLRNCDLAFMGVIDGFTTKMKMVVYIGLVIA
                     LPVILYQLWMFNMPGLTPRERKYAVPFVASSLILFVTGGIFAYLVLPRGLQFLLGFAG
                     EGVGPILTFDRYFGFVVLVLLAFGVSFLLPVLLVFLEAVGVLTPQLLASWRRWAFLGI
                     FVFAAIITPSADFLTLFALAIPMYLFYEAAI"
     gene            complement(745..1128)
                     /locus_tag="VLS86_00280"
     CDS             complement(745..1128)
                     /locus_tag="VLS86_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HSO48953.1"
                     /translation="MFNIGAQELLVILAIALIVVGPAKLPDLARSLGKGLREFRKVQD
                     DVKDMVRFDLDPSSDRPVSRGPVRPTKPRVGGGPGTSASDESPTTNGTPEAPTSADPD
                     ATSAAPDDDASSADGATSERTADPT"
     gene            complement(1145..1387)
                     /locus_tag="VLS86_00285"
     CDS             complement(1145..1387)
                     /locus_tag="VLS86_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO48954.1"
                     /translation="MPPTWILVWFIIGICSTLALIGLLLFLGWHGVLLGRTFQRLGDE
                     IRPLTDDISRATDRRSRRVDELRTKAAGLRFGKTRR"
BASE COUNT          303 a          453 c          521 g          231 t
ORIGIN      
        1 tgatagcggc ctcgtagaac agatacatcg ggatcgcgag ggcgaacagg gtcaggaagt
       61 ccgcggatgg cgtgatgatc gcagcgaaca cgaagatccc gaggaaggcc catcgtcgcc
      121 acgaggccag cagctgaggt gtcaggacgc ccaccgcctc caggaacacc agcagcaccg
      181 gcagcaggaa cgacacaccg aacgcgagga gcaccaggac gacgaacccg aagtacctgt
      241 cgaacgtgag gatcggtccg accccttctc cggcgaaccc gaggaggaac tgcagccccc
      301 tcgggaggac gaggtacgcg aagatcccac cggtcacgaa gaggatcaac gaagacgcca
      361 cgaaagggac cgcgtacttt cgctcgcgcg gtgtgaggcc cggcatgttg aacatccaga
      421 gctgatacag gatgacgggg agtgcgatca ccagaccgat gtagacgacc atcttcatct
      481 tcgtggtgaa gccgtcgatc acacccatga aggcgaggtc gcagttcctg agtggaacca
      541 ggctggggtt gctcgccacg aagtcgcagt acggtgcccg aagggcctcc ttcagcggtt
      601 cgtagaggaa ccagcccggg atggcgcaca cggccaacgc gatgagacag atgatgagcc
      661 ggtgacggag ttcaccgagg tggtcgagca gggtcatggt gccggagcgc tcctcgtcag
      721 agcggcggcg gcgagggatg accctcatgt cgggtctgcc gttcgttccg aggtcgcacc
      781 gtccgcgctc gacgcatcgt catcgggcgc agccgaggtc gcgtccgggt cggccgaggt
      841 gggggcctcg ggcgtcccgt tcgttgtggg ggactcatcg ctcgcggacg tcccgggacc
      901 tcctccaacc ctgggtttcg tcgggcgcac gggtcctctc gacaccgggc ggtcggacga
      961 tggatcgagg tcgaaccgga ccatgtcctt cacatcgtct tggaccttgc ggaattcgcg
     1021 cagccccttg ccgagggatc gagccaggtc gggcagcttg gcagggccaa cgacgatcag
     1081 ggcgatggcg aggatgacga gaagctcttg agcgccgatg ttgaacatga gctcggaggc
     1141 gattctacct gcgggtcttg ccgaacctga gccctgcagc tttcgtccgg agctcgtcga
     1201 cccgtcggga ccggcgatcg gttgcccggg agatgtcgtc cgtgagcggc cggatctcat
     1261 cgccgagccg ctggaacgtt ctcccgagga gcaccccgtg ccagccgagg aagaggagca
     1321 acccgatcaa cgccagggtg ctgcagatcc cgatgatgaa ccacacgagg atccaggtag
     1381 gcggcacgca tggatgctac ctagcaggac gtcgcggcgg gcgatgcccg aggggttcag
     1441 gtgccgttgc tggccaagaa ctcgcggatg tacgcgtcgt cgtccaggag gaagtggaac
     1501 tcgatcac
//
LOCUS       DATITW010000027         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_762600, whole genome shotgun sequence.
ACCESSION   DATITW010000027 DATITW010000000
VERSION     DATITW010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762600"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..479
                     /locus_tag="VLS86_00290"
     CDS             81..479
                     /locus_tag="VLS86_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO48955.1"
                     /translation="MSSLHEPGTEVPLQTRAPTALDLFMFSASAWLLHRIHYDTPFTV
                     EHEGHPGLLIHGPLQGVYLVQTAESWLGDSARISSISYRHLAPAYLGDRLECGGTVVG
                     SDDGGIDIELWARKPDGTITTTGKARFERT"
     gene            479..2146
                     /locus_tag="VLS86_00295"
     CDS             479..2146
                     /locus_tag="VLS86_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSO48956.1"
                     /translation="MTTGARVLAEMLDGYGVTHLFHVPAVLRRTMVEIEARTSIKRLR
                     PHGEKPAAYMADGYARASGRPGLCAAQVVGAHNLAAGLRDAYLAHSPVIAFTGGRDPH
                     TKFRRVYQEVDDVPAFETVTKMNVTIDAVERIPDMVRQAFRVATTGAPGPVHIQFAGN
                     EGQIDAEEAEMDVYCEETFARVPPFRPVAPDDVLVRALDLLKAAERPVIVAGGGVRAS
                     GAGPDLVRLAERLGIPVATSLNGKDTIPGHHPLAVGVVGTYSRGSANRVVLDADLVFF
                     VGTETGGMTTHFWKVPPIGTRAIQLDIEPEALGRNYPPEVAINADAKTALNRMTELAD
                     DSSASRTPWAETSAGHVADWRLEYAPLLESGAVPMRPERLCAELTELVPDDGIVVVDT
                     GHAGMWMGGMYDLRRPSQSYIRSAGHLGWAFPASLGAKCARPERPVVCFTGDSGFWYH
                     VAEIETAVRWGINSITVVNNNHSGNQSKRGFDRVYGGKQSEKAREMWVFTEVDFAALA
                     EHIGAIGIRVEKPADLASALDRALSADRPVVLDVVTDIDALAPPAVE"
     gene            complement(2180..>2629)
                     /locus_tag="VLS86_00300"
     CDS             complement(2180..>2629)
                     /locus_tag="VLS86_00300"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HSO48957.1"
                     /translation="YEAPKVRLPGAGGAPEIAAHCREVFVVMEHNSRALVPKVDFVTS
                     LGFGPSGTEREKLGITTKGPTLVVTDLALLQPEPGSNELVVTSLHPGVTSLHPGVTTE
                     EIRAKTGWPVRFSPDLGQTPAPTDTELTVLRELQERTNRAHQKAADG"
BASE COUNT          431 a          886 c          886 g          426 t
ORIGIN      
        1 gtcttcgtcg agatggagtt caccttcacc aaccagcgag gtgctctcgt ggcccgggag
       61 agcttcacca ggatctaccg atgagctcat tgcatgagcc cggcaccgaa gtcccgctcc
      121 agacccgggc tcccaccgcg ctcgacttgt tcatgttcag cgcctcggcc tggctgctcc
      181 accgcatcca ctacgacacc cccttcaccg tggagcacga gggtcatccc gggctcctga
      241 tccacggccc gctacaaggg gtctatctcg tccagaccgc ggagtcgtgg ctcggagact
      301 ctgcccggat ctcttcgatc tcctaccggc acctggcacc ggcctatctc ggtgaccggc
      361 tcgaatgtgg gggaaccgtg gtcgggtccg acgacggcgg aatcgacatc gagctatggg
      421 cccgcaaacc ggacggcacg atcaccacca cgggaaaggc caggttcgag aggacatgat
      481 gaccaccggt gcccgggtcc tggccgagat gctcgacggc tacggggtga cccatctctt
      541 ccatgtcccc gcggtgctgc ggcgaaccat ggtcgagatc gaggccagga cctcgatcaa
      601 gagactgcgt cctcacggcg agaagccggc ggcctacatg gccgacggct atgcccgcgc
      661 ctcgggacgg cccgggttgt gtgccgccca ggtggtgggg gcgcacaatc tggccgccgg
      721 tctccgtgac gcctatctcg cccactcgcc ggtgatcgcc ttcaccgggg gccgggatcc
      781 tcacaccaag ttccgcaggg tgtaccagga agtcgacgac gtccccgcct tcgagacggt
      841 gaccaagatg aatgtgacca tcgatgcggt ggagcggatc cccgacatgg ttcgccaggc
      901 gtttcgtgtt gccaccaccg gcgctccagg accggtccac atccagttcg ccggcaacga
      961 gggccagatc gatgccgagg aagcggagat ggacgtctat tgcgaggaga cgttcgcccg
     1021 ggtgcccccg tttcgcccgg tggcccccga cgacgtcctg gtccgagccc tcgacctgct
     1081 gaaggcggcc gagcgaccgg tgatagtggc cggaggaggc gtccgcgcct cgggcgccgg
     1141 cccggacctg gtccggctgg cggagagact gggcatcccg gtggcgacat cgctcaacgg
     1201 caaggacacg atccctggtc accacccgct ggcagtcgga gtggtgggga cctactcccg
     1261 cggctcggcc aaccgggtgg tgctcgacgc cgacctcgtc ttcttcgtcg ggaccgagac
     1321 cggcgggatg accactcatt tctggaaggt cccgcccata ggcaccaggg cgatccagct
     1381 cgacatcgag cccgaggccc tgggccgcaa ctacccgccc gaggtggcga tcaatgccga
     1441 tgccaagacg gcactgaacc ggatgaccga gctggccgac gattcgtcgg cgtcgaggac
     1501 gccctgggcc gagacttcgg ccggccacgt cgccgactgg cgtctggaat acgcaccgct
     1561 gctggagtcc ggagcggtgc caatgcgccc cgagcggctg tgcgccgagc tcaccgaatt
     1621 ggtgcccgat gacgggatcg tggtcgtcga caccggtcac gccgggatgt ggatgggagg
     1681 catgtacgac ctgcgccggc cgtcgcagtc ctacatccgc agcgccgggc atctggggtg
     1741 ggcctttccc gcttcgctcg gcgccaaatg cgcccggccc gagcgaccgg tggtctgctt
     1801 caccggtgac tcggggttct ggtaccacgt cgccgagatc gagaccgcgg tgcgctgggg
     1861 gatcaacagc atcaccgtgg tcaacaacaa ccattcgggg aaccagtcga aacgaggctt
     1921 cgaccgggtc tacgggggca agcagagcga gaaggcccgg gagatgtggg tgttcaccga
     1981 agtcgacttc gccgctctcg ccgagcacat cggtgccatc gggatcaggg tggaaaagcc
     2041 cgccgacctg gcctccgcct tggaccgggc tctgtcagcc gaccggccgg tggtgctcga
     2101 cgtggtcacc gacatcgacg ccctggcccc accggcagtg gagtgaccca ccggccgctg
     2161 aggttgggag ggcccggcgc taaccgtccg cggctttctg gtgagcccgg ttggtccgct
     2221 cctgaagctc gcgaagcacc gtcaactcgg tatcggtggg ggcaggggtc tgccccaggt
     2281 cggggctgaa tcggaccggc caccctgtct tggcccggat ctcctcggtg gtgacaccgg
     2341 gatgaagcga ggtgacaccg ggatgaagcg aggtgaccac cagctcgttg ctccccggct
     2401 cgggctggag caaggcgagg tcggtcacta ccagcgtcgg ccccttggtg gtgattccca
     2461 gcttctcccg ttcggtgccc gacggcccaa agccgagcga ggtgacgaag tcgaccttgg
     2521 ggaccagcgc ccggctgttg tgctccatga cgacgaagac ctcacgacaa tgtgccgcga
     2581 tctcgggggc cccgccggcg ccggggagtc tcaccttggg tgcctcgta
//
LOCUS       DATITW010000028         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_321967, whole genome shotgun sequence.
ACCESSION   DATITW010000028 DATITW010000000
VERSION     DATITW010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321967"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VLS86_00305"
     CDS             complement(<1..392)
                     /locus_tag="VLS86_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSO48958.1"
                     /translation="MIELQSAVSGALSGSLFALMALGLSMTWGYLKVINLAHFAMILV
                     GGYLSFQLVTSTGFDPITTILLTAPLMAVVGGLVQLGYERFNVSEFGSLLISYGLLII
                     TVQVVSNVWSADFRRLDPSVNPYATESVS"
     gene            complement(494..1828)
                     /locus_tag="VLS86_00310"
     CDS             complement(494..1828)
                     /locus_tag="VLS86_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSO48959.1"
                     /translation="MGRRFRILAVVTAIGLVAGACAGDTATTTTDGGTATTTGPETTE
                     APSTTTASSTTVAAEGDPIVVGGTLSLTGAFGPTGIIHRIAGEMFIERLNASGGLLGR
                     PVEWNLLDDESDGAQVAALYEQLISQDGVDLIMGPYATPNILAAMPVAERYGYALPQH
                     TSVLAPLMEYACQFPGWSIGPEPNVFIPNQIADAMDSLDGAVSTVALLTNESGSTSFI
                     SYGRADVEEPAAITILPERGYEIVADIPYPPGTSEWGSIATQVRDANADLVLMNGLGV
                     EANGLIEAMQQLDYSPPMMFALFPAPGPLLGLGEASEGVMSVSTFEPNEPILERMGPE
                     VREIVDEFATRAEAEGLPYTVFETQAAASWNAWEILAAGVEGSESLEGQAICDQLHEA
                     GADLTFAGSVVFDPEVNNFWDTNEGLKQIQGGDWVMVWPGDIAAGELAGPAG"
     gene            1979..>2162
                     /locus_tag="VLS86_00315"
     CDS             1979..>2162
                     /locus_tag="VLS86_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO48960.1"
                     /translation="MRLTERGVALSELAYLLWCWAGRSGYTKEALMSRSLLRHRIVKV
                     VGIVTVLAMVPLGVMAS"
BASE COUNT          403 a          696 c          682 g          381 t
ORIGIN      
        1 gacaccgact cggtggcata gggattgacg ctcggatcga ggcgcctgaa gtcagcactc
       61 cacacgttgc tcacgacctg gaccgtgatg atgagcagcc cgtagctgat gagcaacgag
      121 ccgaactccg acacgttgaa cctctcgtaa cccaattgga ccagaccacc gaccacggcc
      181 atcagggggg cggtgaggag aatcgtggtg atcgggtcga atccggtgga ggtgaccagc
      241 tggaagctca ggtagccccc gaccaggatc atggcgaagt gggcgagatt gatgaccttg
      301 aggtatcccc aggtcattga caaccccaag gccatcaggg cgaagaggct ccccgacagc
      361 gcgccggaaa cggcggactg gagctcgatc aattacggca ctcctcaggg gccagtcgac
      421 tcgtcagcat gtcgagaccg ggccgtccgt catcgacgga cggcccgaaa tcccgaccac
      481 actgtcgcag agctcagcca gccgggccag cgagctcccc ggcggcgatg tcaccgggcc
      541 acaccatcac ccagtcacca ccctggatct gcttcaggcc ttcgttggtg tcccagaagt
      601 tgttcacctc gggatcgaag acgacagacc cggcgaaggt gaggtccgcc ccggcctcgt
      661 gcagctgatc gcagatggcc tggccttcga gagattcgga cccctcgacc ccggccgcca
      721 ggatctccca ggcgttccat gaggccgctg cctgagtctc gaacacggtg tagggcagtc
      781 cctcggcctc ggccctggtg gcgaactcgt cgacgatctc acgcacttcg ggacccatcc
      841 gctccaggat cggctcgttg ggctcgaagg tggacaccga catgactccc tcggaggcct
      901 cgcccagccc gagcagcggg cccggggcgg ggaagagggc gaacatcatc ggtggtgagt
      961 agtccagttg ctgcatcgcc tcgatgaggc cgttcgcctc gactccaagc ccgttcatca
     1021 aaaccagatc ggcgttggca tcgcgcacct gggtcgcaat cgagccccac tcggaggtcc
     1081 cgggggggta ggggatgtcg gcgacgatct catacccacg ctcgggcaga atcgtgatcg
     1141 cggccggctc ctccacgtcg gcgcgcccgt agctgatgaa gctggtcgag cccgactcgt
     1201 tggtcaacaa cgccaccgtg gacacggccc cgtcgagact gtccatggcg tcggcgatct
     1261 ggttggggat gaacacgttg ggttcagggc cgatcgacca tcccgggaac tggcacgcgt
     1321 attccatcaa tggcgccagc accgaagtgt gctggggcaa ggcgtagcca taccgctccg
     1381 ccaccggcat cgcggccagg atgttgggtg tggcgtaggg gcccatgatc aggtcgacac
     1441 cgtcctggct gatcaactgc tcgtacaagg cggcaacttg tgcaccgtcc gattcgtcgt
     1501 ccagcaggtt ccactccaca ggtcggccga gcaggccacc ggaggcgttc aaccgttcaa
     1561 tgaacatctc gccggcgatc ctgtggatga tgccggtggg accgaatgca ccggtcagcg
     1621 acaaggtgcc accgacgacg atcggatcgc cttccgccgc caccgtcgtg ctcgaggccg
     1681 tcgtggtcga cggtgcctcc gtggtttcgg gtcctgtggt cgtagccgtg ccgccgtcgg
     1741 tcgtagtggt ggcggtgtcg cctgcgcacg caccggccac gagacctatt gcagtgacca
     1801 ccgccaggat tcggaatcgt ctgcccacgt tttcctcctc gtcagtagat gatcctcccc
     1861 ggtcagtctc cctgtcgcgc tctagcgcca ggaatgcgcg cggagtatac gaatactgaa
     1921 cgctcggctc aacaagaggg ggaaaattaa gtatcactgc acaaagacgg tgaatcctgt
     1981 gaggctcact gagcgaggag tcgccctctc cgaactcgcc tatcttctct ggtgctgggc
     2041 cggccggtct ggttacacca aggaggcgct catgtcccgc tcactgctca gacaccggat
     2101 agtgaaagtc gtcgggatcg tgacagtcct cgccatggtt ccgctcgggg tgatggcgtc
     2161 tc
//
LOCUS       DATITW010000029         1654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_241785, whole genome shotgun sequence.
ACCESSION   DATITW010000029 DATITW010000000
VERSION     DATITW010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241785"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="VLS86_00320"
     CDS             <1..1280
                     /locus_tag="VLS86_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HSO48961.1"
                     /translation="RQYGFDTRQIHAGQRPDPETGARAMPIYATTSFVFEDAQQAADL
                     FALQTFGNIYTRIGNPTTAAFEERMASLEGGLGAVATASGQAAQLIAIFTLCNAGDQV
                     VASKSLYGGTYTQFDVTLRRMGIDVVFVDISDLDAVRGAITDKTRAIYGETVGNPAAD
                     ILDIEAVSAVAHEHGVPLIVDNTFATPYLCRPIEHGADIVVHSATKFIGGHGVAIAGV
                     IVESGLFPFDNGRFPGITEPSPAYHDLRFWENFREYAYLTKVRVETLRDTGASLSPFN
                     SFILLLGLETLSLRMARHTRNARLVAEFLRERSEVAWVRYPIFDDNEWTSLAKRYLPE
                     GPGAVFTFGLSGGFEAAAKFIAGVELASHLANVGDAKTLVIHPASTTHQQVPVEERRA
                     AGVSDEMIRISVGLEDIEDILWDLGQALEGAGT"
     gene            1277..>1654
                     /locus_tag="VLS86_00325"
     CDS             1277..>1654
                     /locus_tag="VLS86_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HSO48962.1"
                     /translation="MTTLVQDATALERLQIIQSARTVALVGVSANPLRSSNFVATYLV
                     RTDLEMYPVNPMYDEILGLRSYPSLADLPVVPDIVDVFRRREELPGVVEEAIAIGAKV
                     VWFQLGLRHEEAAATAAAAGLLVV"
BASE COUNT          275 a          554 c          524 g          301 t
ORIGIN      
        1 aacgccagta cggattcgat acccgccaga ttcacgccgg ccagcgcccg gatccggaga
       61 ccggggcacg agccatgccg atctacgcga caacgtcgtt cgtgttcgag gatgcccagc
      121 aagcggccga tctctttgcc ctgcagacct tcgggaacat ctacacccgg atcgggaacc
      181 cgacaacggc tgccttcgag gagcgaatgg cctccctgga gggcgggctg ggcgcggttg
      241 ccaccgcctc gggtcaggca gcccagctga tagcgatctt caccctgtgc aacgccggtg
      301 atcaggtcgt tgcctccaag tcgctgtacg gaggcaccta cacccagttc gacgtgacgc
      361 tgcgccgaat gggcatcgac gtcgtcttcg tcgatatctc cgatctcgat gcggtcaggg
      421 gagccatcac cgataagaca cgggcgatct acggggagac ggtgggcaac ccggcagcgg
      481 acatcctcga catcgaggcc gtgtccgcgg tggcccatga gcacggtgtc cccctcatcg
      541 tcgacaacac ctttgccacg ccctatctct gccgcccgat cgagcacgga gccgacatcg
      601 tggtccactc cgccaccaag ttcatcgggg gccatggtgt ggcgattgcg ggagtgatcg
      661 tcgaatccgg tctgttcccc ttcgacaatg gtcgttttcc cgggatcacc gagccgtcgc
      721 ccgcctatca cgatctcagg ttctgggaga actttcgcga gtacgcctac ctgaccaagg
      781 tgcgggtcga gacgctccgg gacaccgggg cctcgctgtc gcctttcaac tcgttcatcc
      841 tcctgctcgg tctggagaca ctctcgctgc ggatggcgcg gcacactcga aacgcacgtc
      901 tcgtcgccga gtttctccga gagcgctccg aggtggcctg ggtgcgatac ccgatcttcg
      961 atgacaacga gtggacgtcc ctcgccaagc ggtacctccc ggaggggcct ggcgccgtgt
     1021 tcaccttcgg tctatccggc gggttcgagg ccgcggcgaa gttcatcgcc ggggtcgagc
     1081 tcgcgtctca cctcgccaat gtcggcgacg ccaagaccct ggtgatccat ccagcgagca
     1141 ccacccatca gcaggtcccg gtcgaagagc ggcgggcggc cggggtcagc gacgagatga
     1201 tccggatctc agtgggcctc gaggacatcg aggacatcct ctgggacctg ggccaggcac
     1261 tggagggggc gggcacatga ccactctcgt ccaggacgcc acggcgctgg aacggctcca
     1321 gatcatccaa tccgcgcgaa ccgtggctct ggtcggtgtc tcggccaacc cgctgcgctc
     1381 ctcgaacttc gttgcgacct atctggttcg caccgatctc gagatgtacc cggtcaaccc
     1441 gatgtacgac gagattctcg gtctgcgcag ctatccgtcc ctggccgatc tcccggtggt
     1501 gcctgacatc gtcgacgtgt tccgccgccg cgaggagctt cccggtgtgg tcgaagaggc
     1561 gatagcgatc ggggccaagg tcgtctggtt ccagctgggt ctccgtcacg aggaggcggc
     1621 cgccaccgcg gccgcggcgg ggctgctggt ggtc
//
LOCUS       DATITW010000030         1684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_442197, whole genome shotgun sequence.
ACCESSION   DATITW010000030 DATITW010000000
VERSION     DATITW010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442197"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..899
                     /locus_tag="VLS86_00330"
     CDS             228..899
                     /locus_tag="VLS86_00330"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="catalyzes the formation of pyruvate and glyoxylate
                     from 4-hydroxy-2-oxoglutarate; or pyruvate and
                     D-glyceraldehyde 3-phosphate from
                     2-dehydro-3-deoxy-D-glyconate 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HSO48963.1"
                     /translation="MASHTRLEVLSIIAREGVVPVFYDPDVVVTMQVATTLVAGGIST
                     IEFTNRGDGALDVLGELIRDARRRLPELIVGAGSIVDMATAAHVIDLGANFVFGPSFS
                     PEVAGACHLRNVPYVPGCGTMTEILAAYRAGCDYVKLFPAASIGGPAFLEAVRAPCPW
                     VQAIPTGGVEPTVESMRSWYRAGAPAIGMGSKLLPKQLVDARDFDGLRVLVDATVAAI
                     AEARG"
     gene            862..>1684
                     /locus_tag="VLS86_00335"
     CDS             862..>1684
                     /locus_tag="VLS86_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HSO48964.1"
                     /translation="MPPLPPSPRPGADVVALQIKPAETCRWDCLALGEVMLRFDPEWG
                     RIRTTRHFRVSEGGGEYNVARALRKVFGLRTAVVTALVDSEIGHLVEDLMMTGGVDLR
                     HVKWVPHDGVGHESRVGLNFTEKGFGIRAALGVSDRANTAVSKLKPGDVDWNRIFGEE
                     GVRWLHTGGIFAALSGTTAEVALEAMRAAKDFGTIVSYDLNYRPSLWKPRGGHASAVE
                     VNRALAPYVDVMIGNEEDFTSALGFEVPDLDHEITNLDPSAFKAMIGAVLDAFPGL"
BASE COUNT          293 a          516 c          558 g          317 t
ORIGIN      
        1 gtcgccgtgg gcgatcttga ggtccgtccc gcagattccc gtgaacgcga cagcgatgct
       61 gacctgaccc gggcccggtt cggtcgtttc atacgagcga ggtctgaaga caccgtcccc
      121 gtggtagtca atcgccagat gagtcatcgg attcacaaca gcaggctgca agaacgtcag
      181 tatactgccg acgatgacga gggtgcagca gcgagagagc aagtaggttg gccagtcaca
      241 ccagactcga agtactgagc atcatcgccc gcgagggtgt ggtcccggtt ttctacgacc
      301 ccgacgtggt ggtcacgatg caggtggcaa ccacgctggt ggcgggcggc atcagcacca
      361 tcgagttcac caaccgcggt gacggcgcac tcgacgtgct tggggagttg atccgtgatg
      421 cgcgacgcag actcccggag ctcatcgtcg gggcgggatc gatcgtcgac atggcaaccg
      481 cagcacacgt gatcgatctc ggcgcgaact tcgtgtttgg accgagcttc tcgcccgagg
      541 ttgctggcgc ctgccacctg cgcaatgtcc cgtacgtgcc cggctgcgga acgatgaccg
      601 agatcctcgc cgcctatcgc gccggatgcg attacgtcaa gctcttcccg gcggcctcga
      661 tcgggggtcc tgcgtttctc gaggcggtgc gcgctccgtg cccttgggta caggctatac
      721 ccactggtgg cgtcgagccg accgtggaat cgatgcgctc ttggtatcga gctggcgctc
      781 ctgcgattgg catgggatcg aaactgcttc ccaagcagct ggtggatgcg agagatttcg
      841 atggtctccg ggtgctggtc gatgccaccg ttgccgccat cgccgaggcc aggggctgac
      901 gtcgtggccc tacagatcaa gccggccgag acatgccgtt gggactgtct cgcccttggt
      961 gaggtgatgc ttcgcttcga tcccgagtgg ggtcggatca ggaccacccg gcacttcagg
     1021 gtcagtgagg gtggtggcga gtacaacgtg gcccgtgcgc tcagaaaggt cttcgggctg
     1081 cggaccgccg tcgtcaccgc gctcgtcgac agcgagattg gtcatctcgt cgaggatctc
     1141 atgatgaccg gcggagtcga ccttcgtcac gtgaagtggg taccccacga cggcgtcggg
     1201 cacgaatcgc gggtcgggct caacttcacc gagaagggat tcggcatccg ggcggccctc
     1261 ggggtgtccg accgggcgaa caccgccgtc tccaaactga agccgggcga cgtcgactgg
     1321 aaccggatct tcggcgaaga gggggtgcgg tggctccaca ccggcggcat atttgccgcc
     1381 ttgtccggga ccacggccga ggtggctctc gaggcgatgc gagccgcaaa ggacttcggc
     1441 accatcgtct cctacgacct caattaccgg ccgtcgctgt ggaagccccg cggtggacat
     1501 gccagtgccg tggaggtcaa tcgagctctg gctccatacg tcgatgtgat gatcgggaac
     1561 gaagaagatt tcacttcggc gctcggcttc gaggtccccg acctcgatca cgagatcacg
     1621 aacctggacc cgtccgcctt caaggcgatg atcggggcag tcctcgacgc cttcccgggt
     1681 ctcg
//
LOCUS       DATITW010000031         2989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_201849, whole genome shotgun sequence.
ACCESSION   DATITW010000031 DATITW010000000
VERSION     DATITW010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201849"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VLS86_00340"
     CDS             complement(<1..423)
                     /locus_tag="VLS86_00340"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSO48965.1"
                     /translation="MNIVDLTVPIGKDTHSPPSVNQPIGIKEYHKEPGFWMVSEITMM
                     LHSGSHVDFSKHYVEDGETAEKVLLDRTCGEAVIVDLTPVEPDHDITPDDLLSAAPVI
                     RPGDIVLVRTGWSDVAWGEYPRYYVGSPACTPEAAHWLV"
     gene            582..1658
                     /locus_tag="VLS86_00345"
     CDS             582..1658
                     /locus_tag="VLS86_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO48966.1"
                     /translation="MIERSWDLTVEMVAAADRNETPAWVRTLLSLAGTLVAMAALVWL
                     LGANPLEVVEAIVLGALGNPFNIGQTVVITGILIMTALAAAIPFSARLWNVGGEGQMT
                     VGAVAAAVLGIVLPGTWSSWLLVPVVVMGSMAAGALYGAVPGWLKARFDASEIVTSLM
                     LNFVAASLAAWVIFDVYPEGFVQRTEAIHVNAELPRPIDGSLVDVGIIIAVAVAVIAW
                     FVMKQTRLGFSIRAIGANTSASRLSGIRTERVTVQTFLMAGAFAGLAGSLVVQGRDHS
                     LLQDFSANLGFLGIGVALVARLHPLWIIASASFFAILRVGSNSLQAGAGLSPVVGEIV
                     IATFVILLMVTKVIKFRYPESVDA"
     gene            1651..2574
                     /locus_tag="VLS86_00350"
     CDS             1651..2574
                     /locus_tag="VLS86_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO48967.1"
                     /translation="MPSEALVLAFFVIGIQSMVPIVMAALGEAVAEKSGVLNVGIEGM
                     MLIGAFATAYMGITRTSAGLGILTAIVAGCVFGTLLAFLYVNRGTDQIVTGLMFNIFA
                     VGLTGTLHSLYLAGQNGETLTGISIPGIGSIPWVGEILNKQNLMLYLAIGMAVGVWYL
                     LNKTWYGLYARAAGERPLAAESGGLDVLRLRYPAVIVGSVLAALGGSALVLATSGGFV
                     PGMTAGRGFIALGVVVLAKWRPFWIMIYALGFGLTQGLQFLAAQVPALQSVPRQLWVA
                     LPYLVTVVAVVFAPGSTYPAAVGIPYRRAGA"
     gene            2645..>2989
                     /locus_tag="VLS86_00355"
     CDS             2645..>2989
                     /locus_tag="VLS86_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO48968.1"
                     /translation="MLLHGGTPQLEEQMRTSKPRLAPRWLLLVTAIAMVLAACGGDTG
                     ESTTTAGSTETTAGSTDTTGGSTDTTAGSTDTTAAPDGESYQVALIYPGTADDLSWSN
                     AWFDGAEQAMEAN"
BASE COUNT          482 a          922 c          958 g          627 t
ORIGIN      
        1 gaccagccag tgggccgcct ccggggtgca tgccggcgac ccgacgtagt aacgggggta
       61 ctcaccccat gccacatctg accagccggt cctgaccaac acgatgtcgc cgggccggat
      121 cactggggcg gcggacagca ggtcgtcggg cgtgatgtca tggtccggct cgaccggggt
      181 aagatcgact atcaccgcct cgccacaggt gcggtcgagg agaaccttct ccgccgtctc
      241 cccatcctcc acatagtgct tcgaaaaatc cacatgtgag cctgagtgaa gcatcatcgt
      301 gatctcggag accatccaga acccgggttc cttgtggtac tccttgatcc cgatcggttg
      361 gttcaccgac ggcgggctgt gggtgtcctt cccgatcggg acggtcaggt cgacgatgtt
      421 catgcccctc ccatcgcagt gccgagcgga gcgcaggctg ctcgggcgac ttcaataaca
      481 ctctatcttc cttcacgagg ctcaatccaa gatggtacgg tcgccgcgat cgcgagggac
      541 ctgctgctcc cccgggaggc cggtccgggc cttatggaat cgtgatcgag aggagctggg
      601 acttgacggt ggagatggtc gcggccgcag acaggaatga gactcctgcg tgggttagga
      661 ccctcttgtc tctggccggc acgttggtgg cgatggcggc gctggtttgg ctgctgggcg
      721 ccaaccctct ggaagtggtc gaggccatcg tcctcggcgc cctcggaaac cctttcaata
      781 tcggtcaaac ggtcgtcatc acgggaatcc tcatcatgac cgcgttggcg gcggctatcc
      841 cgttctcggc ccgcctctgg aatgtgggtg gggagggaca gatgaccgtt ggcgcagttg
      901 cggccgccgt gctcgggatc gtcctaccgg gcacgtggtc gtcgtggctg ctggtcccgg
      961 tagtcgtgat gggatcgatg gcggccggtg cgctgtacgg cgcggtcccc ggctggctca
     1021 aagcccgctt cgatgccagt gagatcgtca ccagtctcat gctcaacttc gtcgccgcca
     1081 gcctggcggc ctgggtcatc ttcgatgtct accccgaggg cttcgtccag cgtaccgagg
     1141 ccatccacgt gaacgcggag ctcccccggc cgatcgatgg ctccttggtc gacgtcggaa
     1201 tcatcattgc cgtcgccgtc gcggtgatcg cctggtttgt catgaagcag accaggttgg
     1261 ggttctcgat ccgagcgatc ggcgccaaca cctcggcttc tcgactgtcc ggcatacgaa
     1321 ccgaacgtgt cacggtccag acgttcctca tggccggtgc gttcgccggg ctcgccggat
     1381 cgctggtcgt ccaggggcgc gatcactccc tcctgcagga cttctcagcc aacctcggtt
     1441 tcctcggcat tggggtggca ctcgtggcga ggcttcatcc tttgtggatc atcgcctcgg
     1501 cctcgttttt cgccatcctg cgcgtcggct cgaacagcct tcaggctggc gccgggttgt
     1561 cacctgtggt gggcgagatc gtgatagcca ccttcgtgat cctcttgatg gtgaccaagg
     1621 tcatcaagtt cagatatccg gagagcgtcg atgcctagtg aggctctggt cctcgcattt
     1681 ttcgtgatcg ggatccagtc gatggtgccg atcgtgatgg cggcgttggg cgaggccgtc
     1741 gccgagaagt cgggggtcct caatgtgggc atcgagggga tgatgctgat cggtgccttc
     1801 gctacggcat atatggggat cactagaacc agtgcggggc tcggaatcct gaccgccatc
     1861 gtggccggat gcgtgttcgg caccctgctc gccttcctct acgtgaatcg gggcacggac
     1921 cagatcgtca ccggcttgat gttcaacata ttcgcggtgg gcctcaccgg aaccctccac
     1981 tcgctgtacc tggcggggca gaacggagag acgctgacgg ggatcagcat tccgggcatc
     2041 ggctcgatcc cctgggtcgg agagatcttg aacaagcaga acctcatgtt gtatctcgcc
     2101 attggaatgg ctgtcggcgt gtggtacctg ctgaacaaga cctggtacgg cctctacgcg
     2161 cgagcggctg gcgaacggcc cctcgctgcg gagtccggcg gattggacgt cttgcgtctc
     2221 cgttacccgg ctgtgatcgt ggggtcagtg ctggccgctc ttggcggctc ggctctggtc
     2281 ctagccacct cgggaggttt cgtgccgggt atgactgccg ggcgggggtt cattgccctg
     2341 ggggttgtcg tgctcgcgaa atggcgacct ttctggatca tgatctatgc cctcggcttc
     2401 ggtttgaccc agggtcttca attccttgcg gcccaagtcc cggctctgca gtcggtaccc
     2461 agacagctct gggtggccct tccctatctc gttaccgtcg tagccgtcgt cttcgctcca
     2521 ggctcgacct acccggcggc cgtcggcatc ccatatcgcc gggcaggcgc ttgaccctgc
     2581 aattagtcgt ttcctcccag atttctccga acacccctcg tttgctgtcg gtaaagtaga
     2641 tcgagtgctg ctgcacggcg gcacaccaca attggaggag cagatgcgaa catcaaaacc
     2701 gagattggct ccccgatggc tgttgttggt gacagcaatc gcgatggtcc ttgccgcgtg
     2761 tggtggcgat accggggaat cgaccacgac cgccgggagc accgaaacca ccgccgggag
     2821 caccgatacc accggtggga gcaccgatac gaccgcgggg agcaccgata ccacggcggc
     2881 gccggacggc gagtcatacc aggtggctct gatttaccca gggaccgccg acgatctctc
     2941 ctggtcaaac gcatggttcg acggggctga acaggcgatg gaagccaac
//
LOCUS       DATITW010000032         1656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_81741, whole genome shotgun sequence.
ACCESSION   DATITW010000032 DATITW010000000
VERSION     DATITW010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81741"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..>1656)
                     /gene="ilvD"
                     /locus_tag="VLS86_00360"
     CDS             complement(161..>1656)
                     /gene="ilvD"
                     /locus_tag="VLS86_00360"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSO48969.1"
                     /translation="RHAKTGVASAGGVGLVFSTIAVSDGISMGHEGMRASLVSREVIA
                     DSVELVMHAERFDALVAIAGCDKSLPGMLMAAARLDLPAIFLYGGSSLPGHYAGRDIS
                     IVDVFEGIGAESEGRITIDELLELERAACPGAGSCAGMFTANTMASVGEAIGMSLPGS
                     ASVPAVDPRLRGYAERSGEAVMRLLEDGVRPRDIMTRPAFENAITTVMALGGSTNAVL
                     HLLAIAHEAEVGLELEDFDRISRRTPHIGDLKPFGRYHMVDLDRVGGVPIVLRALLDE
                     GLINGDTLTVTGHTMAENLAEIVFNHEQDVIAPTSAPLAPEGGIAVLRGNLAPGGAVV
                     KVAGIDLDVFEGPARVLDGEQGAIQALFDHQIKSGDVVLIRTEGPKGGPGMREMLQVT
                     AALKGAGLGKEVLLVTDGRFSGGTTGLCIGHVAPESEAGGPMALVAEGDRVRVDVPAR
                     RLDVLVDQQVLDDRATTWSPHPARYETGALAKYARLVGSAETGAVTG"
BASE COUNT          302 a          567 c          513 g          274 t
ORIGIN      
        1 gtctatgcgt tcgagcagcg cattcacatc gacatgctcg aggaccaagt ccgggtcgac
       61 gacatcgagt acccgctcgc tcgctccacg ggcggcgcgc ttgaagaacc ctcgtttttc
      121 cctctcttcg gggatcggct ctgcgctcac cccggagaag ctagccggtg accgcccccg
      181 tttcggccga accgacgaga cgggcatatt tggcgagagc accggtctca taccgcgccg
      241 ggtgtggtga ccaggtcgta gcgcggtcat cgagcacctg ttggtcgacg aggacgtcga
      301 ggcgtcgggc ggggacgtcg acgcggacgc gatccccctc ggctacaagc gccatcgggc
      361 cgccggcctc cgattcgggg gcgacatggc cgatgcaaag cccggttgtg cctccggaga
      421 acctcccgtc tgtcaccagg agcacctcct tgccgagacc ggcgcccttg agggccgctg
      481 tgacctggag catctcccgc ataccgggcc cgcccttcgg cccctcggtc ctgatcaaga
      541 ctacgtcgcc agacttgatc tgatggtcga acaacgcctg aatcgcacct tgctccccat
      601 ctaatacccg agccggtccc tcgaacacat cgagatcgat tccagccacc ttgaccaccg
      661 caccaccggg ggcgagattg cccctgagga ccgcgatgcc gccctccgga gccagaggcg
      721 cagacgtagg cgctatcaca tcctgttcgt ggttgaagac gatttccgcc aggttctcgg
      781 ccatggtgtg accggtgacg gtcagagtgt caccgttgat caagccctcg tccaagaggg
      841 cgcgcaggac gatcggcact ccccccaccc gatccaaatc gaccatgtgg tatctcccga
      901 acggcttgag atcgccgata tggggggtgc gacgggagat ccggtcgaaa tcctccagct
      961 ccaggccgac ctccgcctcg tgggcgatgg ccaggagatg aaggacggca ttggtcgagc
     1021 caccgagggc catgaccgtg gtgatggcgt tctcgaaggc aggtcgcgtc atgatgtccc
     1081 ggggacgcac gccatcctcg aggagtctca tcaccgcctc gcccgatcgc tcggcatagc
     1141 cacgaaggcg agggtcgacg gcggggaccg aggcggagcc ggggagggac atccctatcg
     1201 cctcgccaac cgacgccatc gtgttggcgg tgaacattcc cgcacaggac cccgctccgg
     1261 ggcaggcagc tcgctcgagt tccaggagct catcgatggt gatgcgaccc tcggactctg
     1321 cgccgatccc ctcgaacacg tccacgatcg agatgtcccg cccggcgtag tggccgggaa
     1381 gactggaccc gccgtagagg aagatggcgg ggagatcgag tcgggccgcc gccatcaaca
     1441 tccctggtag cgacttgtcg catcccgcga tggcaaccaa ggcgtcgaat ctctcggcat
     1501 gcatcaccag ctcgaccgag tcggcgatga cctctcgaga gaccaatgag gccctcatgc
     1561 cctcgtgacc catcgagatg ccatcggaga ctgcgatcgt cgagaagacc agacccactc
     1621 ccccggccga cgccacaccg gtcttggcgt gcctcg
//
LOCUS       DATITW010000033         1846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_201967, whole genome shotgun sequence.
ACCESSION   DATITW010000033 DATITW010000000
VERSION     DATITW010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201967"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1846
                     /locus_tag="VLS86_00365"
     CDS             <1..>1846
                     /locus_tag="VLS86_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HSO48970.1"
                     /translation="LPLGREVAAYLDGEDLVAFDNQRFVAAPLPGSLKVRLHGESAFF
                     IEQILEAIPDVDAGVAPGEDVDFEIYSGVAVPLAPTEPFIAIDSPGGAPGITPAGRVE
                     DPIPTLVTDDPLLEDVDVSRIAIADAQVVEVEGGEVLLAAPGAPLIVRGETNGVDYFY
                     IAFTLEQSNLPGNVAFPIIASRMVGELASTDGAATSLTVGDRVPVGSLGGAVVDPRGN
                     RQTVPVGESAPATDQAGFWVVESTEQEPVVIAVNADTDESHLAPRTSLPGLRPAPEGA
                     GDEGTNTAQVARSLLLWVVGALLVVLGVEYWVSRRTRGVSDRQWRWGTALRIFIVGLL
                     VLSVIDPDFSRSDNSVTTVFVVDVSDSIGDSVSESRSWVEAAITDAGDGRWAVVEVGG
                     DARVATPRGNLPYSGAADVDVTATNLARGLRLGESLLTGETRERLVLVSDGRSNTGDL
                     SAEIDRLKALGVVVDVHTVEAAVGTDAAVATIDVPSEVNAGESFEAVVEILSTVSASG
                     EVELLANGEVVGRQSASLEPGSNLVTFPVDAGEPGLQDLTARVQVSGDSTRQNDSTTT
                     GVQVQGPASVLVIEGEEGEGQIIVDVLGSKGLVVETITMEELPGIQELS"
BASE COUNT          286 a          584 c          673 g          303 t
ORIGIN      
        1 ctgccgctgg gccgggaggt ggcggcctat ctcgatggtg aggacctggt cgccttcgac
       61 aatcagcggt ttgtggccgc ccctcttccc ggcagcctca aggtgcgtct ccacggagag
      121 agcgccttct tcatcgagca gatcctggag gcgatacccg acgtcgatgc gggtgtcgct
      181 cctggcgagg atgtcgactt cgagatctac tccggtgtgg cggtgcccct ggccccgacc
      241 gagccattca tcgccatcga cagcccgggt ggggcgcccg ggatcacgcc cgccggccgg
      301 gtcgaggacc cgatcccgac cctggtcacc gacgatccgc tgctcgagga cgtcgacgtg
      361 tccaggatcg ccatcgccga cgcccaggtg gtcgaagtcg agggaggcga ggtgctcctg
      421 gccgcgccgg gcgccccgtt gatcgtgcgg ggcgagacga acggagtcga ttacttctac
      481 atcgcgttca ccctcgagca gtcgaacctc ccggggaacg ttgcgtttcc gatcatcgcc
      541 agccgcatgg tcggtgagct ggcctccacc gacggtgccg cgacatcgct gacggtcggg
      601 gaccgggtgc cggtggggtc gctcgggggc gcggtggtag acccgagggg gaaccgacag
      661 accgtcccgg tcggcgagtc ggcgccggcc acggaccagg ccgggttctg ggtggtggag
      721 agcacggagc aggaaccggt cgtgatcgcg gtcaatgccg acaccgatga gtcccacctg
      781 gcgcctcgga ccagccttcc cggtctgagg ccggcaccgg agggggcggg tgacgagggg
      841 accaacacgg cgcaggtggc acgatcgctt ctcctctggg tcgtcggcgc cctgctggtg
      901 gttctgggcg tcgagtactg ggtcagccgt cgcacccggg gggtatccga ccgccagtgg
      961 cgttggggca ccgccctcag gatcttcatc gtcgggctgc tggtcctctc cgtgatcgac
     1021 cccgacttca gccgctccga caactcggtc acgaccgtct tcgtggtcga tgtctccgac
     1081 tcgatcggcg actcggtgag cgaatcgcgg tcctgggtcg aggctgccat caccgacgcc
     1141 ggggatggga gatgggcagt cgtcgaagtg gggggcgacg cacgagtcgc aactccccga
     1201 ggcaacctcc cctactcggg cgccgcagac gtggacgtga cggccaccaa tctggcgcgg
     1261 gggcttcgac ttggggagtc gctcctgacc ggcgagacca gggagcgact ggtcctggtc
     1321 tccgatggcc gctccaacac cggcgatctc tcggccgaga tcgaccggct caaggccctg
     1381 ggcgtggtgg tggacgttca caccgtcgag gcagctgtgg gcaccgacgc agcggtcgcc
     1441 acgatcgacg tcccgagtga ggtcaacgcg ggtgagtcgt tcgaggcagt ggtggagatc
     1501 ttgtccactg tctccgcatc gggcgaagtg gagttgctgg cgaacggaga ggtggtcggg
     1561 cgacagagcg ccagtctcga gccgggaagc aatctggtca ccttcccggt cgacgccggg
     1621 gagccggggt tgcaggacct gaccgccagg gtccaggtgt cgggggactc cacgcgtcag
     1681 aacgactcga cgacgaccgg ggtacaggtc cagggcccgg cgagcgttct cgtgatcgaa
     1741 ggtgaggaag gtgaaggcca gatcatcgtc gacgtgctcg ggtcgaaagg cctggtggtc
     1801 gagaccatca ccatggagga attgcccggg atccaagagc tctccg
//
LOCUS       DATITW010000034         1722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_642645, whole genome shotgun sequence.
ACCESSION   DATITW010000034 DATITW010000000
VERSION     DATITW010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642645"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VLS86_00370"
     CDS             <1..223
                     /locus_tag="VLS86_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HSO48971.1"
                     /translation="FLAVADALLLLGSTVAVWPASDVVYRAAHRSIPIVIINRGETEA
                     DLLAAAKIDAGIGEVRPDLVEAVLAAPIQ"
     gene            294..>1722
                     /locus_tag="VLS86_00375"
     CDS             294..>1722
                     /locus_tag="VLS86_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSO48972.1"
                     /translation="MSDYAPRLDDINFTLGHVAGLEEVSKLNGYQHADPETVAAILEE
                     AGRFFSEVMAPLNQIGDRQGSVLGEDGSVRTPDGFKEAYQKFVESGWAAAHLPEEWGG
                     GGLPYMVGVVIEEMFKTANMAFALCPTLTHGAVEALRIHGSEALLGAYMEKLVRGEWT
                     GTMNLTESEAGSDLGALRTRAEPQADGTYRIFGTKIFITWGDQDLTENIIHFVLARLP
                     DSAPGTKGISMFMVPKFILDENGNPGQRNDYKIVSLEHKLGIHASPTCVVSFGDAGEG
                     AVGYVVGEAGSGMRSMFTMMNAARIGVGMEGVAVGERAYQRALRYARERVQGRAIGAS
                     DTARVPILQHPDVRRMLLTMKANVEAMRSLLYVTAAEADRHIRGETEDAREVAGSRLS
                     LLTPIVKAWCTDLGVEVASLGIQVHGGAGYIEETGAAQYLRDSRIAPIYEGTNGIQAI
                     DLVLRKLPLENGAVVTALITEMTEVV"
BASE COUNT          314 a          562 c          558 g          288 t
ORIGIN      
        1 cttcctggcc gtggccgacg ccctcctcct cctcgggtcc acggtggcgg tgtggccggc
       61 ctcggacgtg gtctatcggg cagcacaccg ctcgataccg atcgtcatca tcaatcgcgg
      121 cgagaccgag gccgacctcc tcgccgccgc caagatcgac gccggaatcg gagaagtgcg
      181 tccggacctg gtcgaagccg tcctcgctgc acccattcaa taattgggac agggacgggt
      241 cgtatgtgac ccccccctta tcccaatcac gggagagggt ttagccttta ggcatgtccg
      301 actacgcccc gcgtctcgac gacatcaact tcaccctggg ccatgtggcc gggctcgagg
      361 aggtgtccaa gctcaacggt taccagcacg ccgaccccga gacggtggcc gccatcctcg
      421 aggaagcagg gcggttcttc tcagaggtga tggcccccct caaccagatc ggggaccgcc
      481 agggatcggt gctgggcgag gacgggtcgg tccggacccc ggacggtttc aaggaggcgt
      541 atcagaagtt tgtggagtcg ggttgggccg ccgcccatct ccccgaggaa tggggcgggg
      601 gcgggctccc ctacatggtg ggagtagtca tcgaggagat gttcaagacc gccaacatgg
      661 cctttgcgct ctgtcccact ctcacccacg gggcagtcga ggcgcttcgc attcacggat
      721 ccgaagccct cctgggtgcc tacatggaga aactggtccg tggcgagtgg accggcacca
      781 tgaatctcac cgagtccgaa gcagggtccg atctgggggc cctccgcacc agagccgagc
      841 cccaagccga tggcacctat cgcatcttcg ggaccaagat cttcatcacc tggggcgacc
      901 aggacctgac cgagaacatc atccatttcg tgcttgcccg gctccccgat tccgcccccg
      961 ggaccaaggg gatttcgatg ttcatggttc ccaagttcat cctcgacgag aacgggaacc
     1021 cggggcagcg caacgactac aagatcgtct cgctcgagca caagctgggg atacacgcct
     1081 caccgacctg tgtggtctcc ttcggcgacg ccggcgaggg ggcggtcggc tacgtggtgg
     1141 gcgaggccgg ctcggggatg cgcagcatgt tcaccatgat gaatgccgct cggatcggcg
     1201 tcggcatgga gggagtcgcc gtcggggaac gggcctatca acgtgccctc cgctacgcca
     1261 gggagcgggt ccaaggacgt gcgatcgggg ccagcgacac cgccagagtt cccatcctcc
     1321 aacaccccga tgtgaggcgg atgctgctga ccatgaaggc gaacgtggag gccatgcggt
     1381 ctctcctcta cgtcaccgcg gcagaggcgg accgacacat tcgcggggag accgaagacg
     1441 cacgcgaggt ggccgggagc cggctctctc tgctcacacc catcgtcaag gcctggtgca
     1501 ccgacctcgg ggtggaggtg gcctctcttg gaatccaggt ccacggtggg gccgggtaca
     1561 tcgaggagac cggtgccgcc caataccttc gtgactcgcg tatcgccccc atctacgaag
     1621 ggaccaacgg gatccaggcc atcgacctgg tgctccgcaa gctgcccctc gagaacgggg
     1681 cggtggtgac cgcgctgatc accgagatga ccgaagtggt ca
//
LOCUS       DATITW010000035         1797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_402291, whole genome shotgun sequence.
ACCESSION   DATITW010000035 DATITW010000000
VERSION     DATITW010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402291"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /gene="sucC"
                     /locus_tag="VLS86_00380"
     CDS             <1..293
                     /gene="sucC"
                     /locus_tag="VLS86_00380"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859073.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HSO48973.1"
                     /translation="GGADEAQVTTAFQIITQDPNVRGIFVNIFGGIMRCDVIATGIVA
                     AVKNVGLDVPLVVRLEGTNVEQGKQIIRDSGLDVVAADSLRDGAEKIVELVS"
     gene            290..1213
                     /gene="sucD"
                     /locus_tag="VLS86_00385"
     CDS             290..1213
                     /gene="sucD"
                     /locus_tag="VLS86_00385"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HSO48974.1"
                     /translation="MSILVGKDTRVLIQGLGKTGQFHTDKAIAYGTQMVGAVHPSRAG
                     TTSHFEGETDQSGVPGRGDTYSVDLPVFATVSEAVEATGATASVVYVPPPFAADAIME
                     AAAAGLDLVVAITEGVPVSDMIKAKRYLADKVTRLIGPNCPGVITPGECKIGIMPGYI
                     HQRGRIGVISRSGTLTYEAVYQLTLNGLGQTTAVGIGGDPVNGTSFVEMLEMFEDDPE
                     TEGVIMIGEIGGTAEEDAAEFVAKHMSKPVAGFIAGTTAPAGKRMGHAGAIVSGGKGT
                     AEAKIEAMEVAGIVMAETPTDMGEAMARALG"
     gene            1200..>1797
                     /locus_tag="VLS86_00390"
     CDS             1200..>1797
                     /locus_tag="VLS86_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HSO48975.1"
                     /translation="MPSAEFLAAIRSGGQRILELARSDPSRPVPQYPGWSMTDLATHV
                     ASIHGRTTLICHDHPTERISAPRLPEGQDVFDWYAANLGEMLTALEETDPEIPVWGFA
                     DSSTIAFWERRMVVETGVHRWDAEQAFGDEMPLPEVVAAAGLDEYAGMWVPRLGDMPE
                     IEVTATDLGRTWVFGSLAPRSEVKGTASDLYLRLMSRSS"
BASE COUNT          324 a          552 c          628 g          293 t
ORIGIN      
        1 gaggtggggc cgacgaggca caggtgacca ccgcgttcca gatcatcacc caggacccga
       61 acgtcagggg gatcttcgtc aacatcttcg gggggatcat gcgctgtgac gtgatcgcga
      121 ccggcatcgt cgctgcagtg aagaacgtgg gtctcgatgt ccccctggtg gtccgtctcg
      181 aggggaccaa cgtcgaacag ggcaagcaga tcatccgtga ctccggtctc gacgtggtag
      241 ccgccgacag tctcagggac ggcgccgaga agatcgtgga gttggtgagt tgagcatcct
      301 ggtaggcaag gacacccggg tcctgattca gggtctgggc aagaccggtc aattccacac
      361 cgacaaggcc atcgcctacg ggacgcagat ggtcggcgca gtccatccgt ctcgtgccgg
      421 caccacgtcg catttcgagg gggagaccga ccaatcgggt gtccccggcc ggggtgacac
      481 ctattcggtc gacctcccag tgtttgccac ggtcagcgag gcggtggagg ccacgggcgc
      541 caccgcctcg gtggtctacg tgcccccgcc gttcgccgcc gacgccatca tggaggcggc
      601 ggcagccggc ctggatctgg tggtggccat caccgagggt gtgccggtct ccgacatgat
      661 caaggccaag cgctatctgg ccgacaaggt cacccgtctc atcggcccca actgcccggg
      721 ggtgatcact ccgggtgagt gcaagatcgg gatcatgccc ggctacatcc accagcgggg
      781 gaggatcggg gtgatctcca ggtcggggac cctcacctat gaggcggtgt atcagctgac
      841 cctcaacggg ctggggcaaa ccaccgcggt cgggatcggg ggtgatccgg tcaacgggac
      901 cagcttcgtc gagatgctgg agatgttcga ggacgacccg gagaccgagg gtgtgatcat
      961 gatcggtgag atcgggggga cggccgaaga ggacgcagcc gagttcgtcg ccaaacacat
     1021 gagcaagccg gtcgccgggt tcatcgcggg gaccacggct ccggcaggca agcgaatggg
     1081 tcacgccggt gccatcgtct ccgggggcaa gggaacggca gaggccaaga tcgaggccat
     1141 ggaggtggca gggatcgtca tggccgagac accgacagac atgggggagg ccatggcccg
     1201 tgccctcggc tgagttcctg gcggcgattc gctccggagg gcagcggatt ctcgagctgg
     1261 cccgcagcga cccgagccgg ccggtgcccc aatatccggg ctggtcgatg accgatctgg
     1321 ccacccacgt tgcctcgatc catggtcgga ccacgctgat atgccatgac cacccaaccg
     1381 agagaatctc agcgccccgt ctccccgaag gtcaggatgt cttcgactgg tacgcggcca
     1441 atctcggcga gatgctgacc gctctggagg agacggaccc cgagataccg gtgtggggct
     1501 ttgccgactc atcgaccatc gccttctggg agcgacggat ggtcgtcgag accggagtcc
     1561 atcgctggga cgccgagcag gcatttggcg acgagatgcc cctgcccgag gtcgtcgccg
     1621 cagccgggct ggacgagtat gcgggcatgt gggtgccccg actgggtgac atgcccgaaa
     1681 tcgaggtgac tgccaccgac ctcggccgga cctgggtctt cgggtcgctc gccccgaggt
     1741 cggaggtgaa gggcacggca tcggacctct atctgaggct gatgtcacgg tcttccc
//
LOCUS       DATITW010000036         2821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_642791, whole genome shotgun sequence.
ACCESSION   DATITW010000036 DATITW010000000
VERSION     DATITW010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642791"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VLS86_00395"
     CDS             <1..830
                     /locus_tag="VLS86_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HSO48976.1"
                     /translation="LASGEDRVVETREIPPDLAVDASFEDPLQSLDAVAFVARALAER
                     LLERLRASGVAPHRVSILVEAAAGEPRERIWRSADPFTEKALSDRVWWQLRAWVESGG
                     VPGGISRLRIAPADLSDEGRQLGLLQDESSRIEAERALARAQALLGPDGVLQGRAQGG
                     RLPQERVAWSRWGEPPVDQDRDPSAPWPGATPSPSPALIPPRLQPLDVDWDDGMPTRV
                     RLGTRWEPVLTWSGPWRLSGRWWAGERDADRYQLVTSVGAFLCVVTGGKSYLAGIYD"
     gene            888..1556
                     /locus_tag="VLS86_00400"
     CDS             888..1556
                     /locus_tag="VLS86_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HSO48977.1"
                     /translation="MLADPSFRKGLRDTALIFFAVAAFGLAYGVLAVEAGLSPWLVLF
                     SSVVIVSGAAQFAMVGLLAAGPIPVLVAATGLGLRHLPMSATLARLIGPQPLGTRLRL
                     AFILVDETFGLSVRAANAGVEDLVAYKTGADLMLYTGWLLGSGVGAWFGAAIDPEAAG
                     VGVLFGLLFLGLAAPMIHRRRDWIVVAAAIVATLVATLTLPPAWQVTVAATAASLVGM
                     VAGD"
     gene            1549..1860
                     /locus_tag="VLS86_00405"
     CDS             1549..1860
                     /locus_tag="VLS86_00405"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HSO48978.1"
                     /translation="MTDLMLILAVAAVTYSSRVAFLIRPRPVPGGALGRFLDVFPLAL
                     FVAIATVGLATPEGSPAVTPGLAAAAGGVLGGIAFRRSLVGILVVGAASFYLVRALLG
                     S"
     gene            complement(1888..2013)
                     /locus_tag="VLS86_00410"
     CDS             complement(1888..2013)
                     /locus_tag="VLS86_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO48979.1"
                     /translation="MANKDKGGKSSKKVAAKDLKQKRQSKKEKKAAADSANRAIR"
     gene            complement(2092..>2821)
                     /locus_tag="VLS86_00415"
     CDS             complement(2092..>2821)
                     /locus_tag="VLS86_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSO48980.1"
                     /translation="VGRTVLTGAAGVIATIVAVAVIAVIAVFDKTSPKIERVIRGWSR
                     VWLTTSGTRLEVDGQENIDPNRSYVVVANHLSALDIMACVLAVPLPIRFLAKKELFRI
                     PVLAQGMRTVGIIEVDREARGAIHAEVNRQSKELIALGRSLIIYAEGTRPRNGVMKPF
                     KKGAFTMAIATQLPVLPLSLHGSYEAWPPGRHLIRGGLIRVVLDKAIETEGLSHSDTG
                     DLRDQVRDVISGRVSEMGGVVSGG"
BASE COUNT          371 a          951 c          954 g          545 t
ORIGIN      
        1 ggttggccag cggggaggac cgagtcgtcg aaaccaggga gatcccaccc gatctcgcgg
       61 tcgatgccag cttcgaggac cctctgcaga gtctcgatgc cgtggccttc gtcgcccgag
      121 ccctggccga gaggctcctc gagcgtctga gagcttccgg ggtcgctccg caccgggtgt
      181 cgatcctggt cgaggcggcg gccggagagc cccgggagcg aatctggcgc tccgccgatc
      241 cgttcaccga gaaggcattg tccgaccggg tgtggtggca gttgcgagca tgggtggagt
      301 cgggcggtgt gcccggtggc atctcccggt tgcggatcgc ccctgcggat ctctccgacg
      361 aggggagaca actgggtctg ctccaggacg agagctccag gatcgaggcc gagcgggcgc
      421 tggccagggc ccaggccctg ctcgggcccg atggcgtcct ccaagggcga gctcagggcg
      481 ggcggttgcc acaggagcgg gtggcctggt cacggtgggg cgagcccccc gtggaccagg
      541 atcgagaccc ttcggccccc tggcctggtg ccacgccctc gccgagcccg gccctgatcc
      601 cgccccgctt gcagccactg gacgttgatt gggacgacgg gatgccaacc agggtccgac
      661 tcgggaccag atgggagccg gttctcacct ggagtggtcc ctggcgtctc agcggccgat
      721 ggtgggcggg ggagagggat gctgaccgct atcagctggt cacctctgtc ggggcatttc
      781 tctgcgtggt gacgggagga aagtcatatc tggccgggat ctacgactga ttcgacccac
      841 cctggccccc tggccgggcc accacccacc accgttatcg tcgtccgatg ctcgccgacc
      901 cgtcctttcg caaggggcta agagacaccg ctctgatctt cttcgcggtg gccgcattcg
      961 gactggccta cggggtgctg gcggtcgagg ccgggctgtc tccgtggctg gtcctcttct
     1021 cgtccgtggt gatcgtctcc ggcgccgccc agttcgccat ggtcgggctg cttgccgccg
     1081 gtccgatccc ggtgctggtc gcggccaccg gtctcgggct gcgtcatctc ccgatgtcgg
     1141 cgacgctcgc ccgtctcatc gggccccagc cgctggggac acgtctccgg ctcgcattca
     1201 tcctcgtcga cgagaccttt ggcctctcgg tgagggcggc caacgccgga gtggaggacc
     1261 tggtcgcgta caagaccggc gccgacctga tgctgtacac gggatggctg ctcggatcag
     1321 gcgtcggagc ctggtttggc gctgcgatcg atcccgaggc cgccggggtc ggggtcctct
     1381 tcggtctgct gttcctcggc ctcgcagccc cgatgatcca cagacgtcga gactggatcg
     1441 tggtcgcggc ggcgatcgtg gccacgctgg tggcgacctt gacgctgccg ccggcgtggc
     1501 aggtgacggt ggctgccacc gccgcctcac tggtggggat ggttgccggt gactgacctg
     1561 atgctgatcc tggcggtcgc ggccgtcacc tactcgagca gggtggcctt cctgatccga
     1621 cctcgaccgg tgccgggcgg agccctgggc cggttcctcg acgtgttccc gctggccctg
     1681 ttcgtcgcga tcgcaaccgt cgggctggcg accccggaag ggtcacctgc ggtcacgccc
     1741 ggtcttgccg ccgccgccgg gggagtgctc ggcggaatcg ccttccggcg ctcgctggtc
     1801 gggatcctcg tcgtcggggc ggccagcttc tatctggtcc gtgccttgct cgggtcctga
     1861 gcaccccgac gcgccgcctc gggcgggcta gcggatcgcc cggttggcgc tgtctgcagc
     1921 cgccttcttt tccttcttcg actggcgttt ctgcttgagg tccttggcgg cgaccttctt
     1981 gctgctcttg ccgcccttgt ccttgttagc catctgtggc ctccctgtgg gtgggatctg
     2041 tgcgatccat caccagtctg tcacgttcgg cgccgaagtt ggcaccaggg ctcatccgcc
     2101 ggagacgact cctcccatct cgctgacgcg gcccgagatc acgtctcgaa cctgatcccg
     2161 gagatcgccg gtgtcggaat ggctcagccc ttcggtttcg atcgccttgt cgagcacgac
     2221 tctgatcagg cccccccgga tcagatgcct gcccggcggc catgcttcgt aggacccatg
     2281 cagagacaac ggaagcaccg ggagctgggt ggcgattgcc atggtgaatg ctcccttctt
     2341 gaacggtttc atcaccccgt tgcgcggtct ggtcccctcg gcgtagatga tcaggcttcg
     2401 ccccaacgca atcagctctt tcgactgtcg attgacctcg gcgtggatcg cgccccgggc
     2461 ctcacggtcg acttcgatga tgccgaccgt tctcatcccc tgtgccagaa ccgggattcg
     2521 aaacagctct ttcttggcca gaaaccggat cggcagcggc acggcgagca cgcaggccat
     2581 gatgtcgagg gccgagaggt ggtttgccac caccacgtag gaccggttgg ggtcgatgtt
     2641 ctcctgccca tcgacctcga gtctggtgcc cgaggtcgtc agccacaccc gactccagcc
     2701 gcggatgacc cgctcgatct tcggcgaggt cttgtcgaac acggcgatga ccgcgatcac
     2761 ggcgactgcg acgatggttg cgatcacacc ggcggcaccg gtgagaacag tcctccctac
     2821 g
//
LOCUS       DATITW010000037         1726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_242186, whole genome shotgun sequence.
ACCESSION   DATITW010000037 DATITW010000000
VERSION     DATITW010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242186"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VLS86_00420"
     CDS             <1..510
                     /locus_tag="VLS86_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HSO48981.1"
                     /translation="HKRNPILSERMTGIARILRGYSQVGLENVALWHERDISHSSAER
                     VVLPDASIALHYRLVKFTDLIANLVVDADRMSANLAASRGLVFSQAVLLALVETGMTR
                     DQAYRIVQRHSMVAWSEARDLRDLLAADPEVPLGDDALETCFSLERVVESTRPVFDRL
                     RGLELEPVA"
     gene            complement(544..918)
                     /locus_tag="VLS86_00425"
     CDS             complement(544..918)
                     /locus_tag="VLS86_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HSO48982.1"
                     /translation="MLAADAYARSLGVELISVDDDEIVVGLEVRPDHLNFLEVGHGGM
                     VFSLADCAFSLGANAAGDRAVAIDTHFVLTAASRLGDRLQARVTEASRGRTIATYRVD
                     VSRGDGRRVGLFTGTVHITPAQ"
     gene            complement(993..>1726)
                     /locus_tag="VLS86_00430"
     CDS             complement(993..>1726)
                     /locus_tag="VLS86_00430"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSO48983.1"
                     /translation="ELTHTVKGKGEQMKLRNLMTRDVITIGPDASLKEAARRMIEARV
                     SGLPVTEADGSLVGIITEADFVTAEADRRHRKRAGLLRLVYKDVALPSHERLVRDVMS
                     TDLVVLGPEADHAEAARLMQTEGVKRIPVVDDDDKLVGLVSRSDVLRVFARSDQEILD
                     EIREHVMGDILWIDPSRIDLRSTEGNVVLRGRLETRSDAELLARLAARVDGVVSVANH
                     LEFEVDNLKLEMSGPPTGMGIPRSW"
BASE COUNT          294 a          592 c          532 g          308 t
ORIGIN      
        1 cacaagcgga acccgatcct ctccgagcgg atgacgggga tcgcccgaat cctccgtggc
       61 tactcacagg tgggtctgga gaatgtcgcc ctctggcacg agcgcgacat ctctcactcc
      121 tcggcggagc gcgtggtgct ccccgacgcc tcgatcgccc tccattacag gctggtgaag
      181 ttcaccgatc tgatcgccaa tctggtggtc gacgccgatc ggatgtcggc caatctcgcg
      241 gccagtcgcg ggctggtctt cagccaggcg gtgttgctcg ctctggtgga gaccggaatg
      301 actcgagacc aggcatatcg catcgtccag aggcactcga tggtcgcctg gagcgaagcc
      361 cgggatcttc gggacctcct tgccgcggac cccgaggttc ccctcggcga tgatgccctg
      421 gaaacctgct tctcgctgga gcgggtcgtc gagagcaccc ggccggtctt cgaccgtctc
      481 cgcggcctcg agctcgaacc ggtcgcttag ggcttgcccc gggcgggcac aaccgctctc
      541 cgctcactgg gccggcgtga tgtgaaccgt gcccgtgaac agacccaccc tgcgaccgtc
      601 gcctctgctg acgtcgaccc ggtaggtcgc gatggtgcgg ccccgggagg cctcggtgac
      661 cctcgcctgg aggcggtcgc ccagccggga agctgcggtg aggacgaaat gggtgtcgat
      721 cgccaccgcc cggtcgccgg cggcattggc gccgagcgag aaggcgcaat ccgccaggga
      781 gaacaccatc ccgccgtgac ccacctcgag gaagttcagg tggtcggggc gtacttcgag
      841 gccgacgacg atctcgtcgt catcgaccga gatcaactcc acacccaggg atctggcata
      901 ggcgtcggcc gcgagcattt gctcgacact cgatcgggag aacccaggca ccaggcggcg
      961 agcctagagg ggaaacccgc tccccgccgt cgtcaccagc ttctgggaat ccccatcccc
     1021 gtcggcggcc cggacatctc gagcttcagg ttgtcgacct cgaactcgag gtggtttgcc
     1081 accgacacca cgccgtcgac cctggctgcc agcctggcca gaagctctgc gtcgcttcgg
     1141 gtctccagcc gtccccggag caccacattt ccctcggtgc tgcggagatc gatccggctg
     1201 ggatctatcc agaggatgtc acccatcaca tgctctcgaa tctcgtccag gatctcctga
     1261 tcggagcggg caaagacccg gagcacgtcg gatcggctca cgaggccaac cagcttgtcg
     1321 tcgtcgtcga ccactggaat ccgcttgaca ccctcggtct gcatgagacg ggcagcctcg
     1381 gcatggtctg cctcggggcc gagcaccacc aaatcggtgc tcatcacatc acgaaccaga
     1441 cgctcatgcg aaggcagagc gacatccttg tagaccagtc gcaacaggcc ggctctcttc
     1501 ctgtgacgac gatcggcctc agcagtcacg aagtcggcct cggtgatgat cccgaccagt
     1561 gagccatccg cctcggtcac aggcagaccg ctcacgcggg cctcgatcat gcgccgcgcc
     1621 gcctctttga gtgatgcgtc gggaccgatg gtgatgacat cgcgtgtcat caggttgcgg
     1681 agcttcatct gttctccttt ccccttcacc gtatgggtga gctcga
//
LOCUS       DATITW010000038         1799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_562849, whole genome shotgun sequence.
ACCESSION   DATITW010000038 DATITW010000000
VERSION     DATITW010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562849"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..986
                     /gene="pdxS"
                     /locus_tag="VLS86_00435"
     CDS             105..986
                     /gene="pdxS"
                     /locus_tag="VLS86_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HSO48984.1"
                     /translation="MQQGTDRVKRGLAEMLKGGVIMDVVTAEQALIAEDAGAVAVMAL
                     ERVPADIRKAGGVARMANPTKVEEIQAAVSIPVMAKCRIGHFIEARVLQSLGVDYIDE
                     SEVLTPADNQHHIDKWDFTTPFVCGARNLGEALRRIGEGAAMIRTKGEPGTGNVVEAV
                     THMRAVTGEIRRLTQLDEEQLMATARDLGAPYELVKEIHETGKLPVVNFAAGGIATPS
                     DAALMMALGCDGIFVGSGIFKSDDPSGFARAIVEATTFWADADVVAKVSRGLGEAMAG
                     LEIDKIPAGEKMAERGW"
     gene            991..1554
                     /gene="pdxT"
                     /locus_tag="VLS86_00440"
     CDS             991..1554
                     /gene="pdxT"
                     /locus_tag="VLS86_00440"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018836750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HSO48985.1"
                     /translation="MPATGVLALQGDFREHLTMLRQVGTDPIPVRTPAELAEVEALVI
                     PGGESTTIGKLADRFGLIDPLRTRIEAGMPVYGTCAGLIFLSAAVTEGEQHLLGVLDA
                     VVHRNAFGRQNESFEEDLDIVGLDTPFRAVFIRAPWVAKLGDGVEVLATIDSHPVMVR
                     QGSILASSFHPELTDDTRIHRMLVDLI"
     gene            1564..>1799
                     /locus_tag="VLS86_00445"
     CDS             1564..>1799
                     /locus_tag="VLS86_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSO48986.1"
                     /translation="MSGHSKWATLKRKKAATDSKRGKLFAKLIRAIEVSARQGGGDVN
                     ANATLAAAVAKARASAVPLDNIERAIQRATGEADT"
BASE COUNT          344 a          542 c          596 g          317 t
ORIGIN      
        1 tttcggttct attccccttc caaaacccgt gggacttgtc ttcactaccg agaagggcca
       61 tcgacccgtc gaggagtaga attgccttca agcaatgagt tgttgtgcaa caaggcaccg
      121 atcgggtgaa gcgtggtctg gcggagatgc tgaagggtgg cgtcatcatg gacgtcgtca
      181 ccgccgagca ggctctgatc gccgaagatg ccggcgctgt tgcagtgatg gccttggagc
      241 gggtcccggc cgacatccgc aaggcagggg gagttgcccg catggccaac ccgaccaaag
      301 tcgaggagat ccaggcggca gtttcgatcc cggtcatggc caagtgccgg atcggccact
      361 ttatcgaagc ccgcgtgctg cagtccctgg gcgtcgacta catcgacgag tccgaagttc
      421 tcactcccgc cgacaaccag caccacatcg acaagtggga tttcaccacg ccgttcgtct
      481 gcggcgcccg caacctggga gaggccctgc gtcgaatagg cgaaggggcc gcgatgatcc
      541 gcaccaaggg cgagcccggg accggaaacg tggtcgaggc cgtcacccac atgcgtgccg
      601 tcaccggcga gattcgtcga ttgacccagc tcgatgagga acagctgatg gccactgccc
      661 gggacctcgg ggccccctat gaattggtga aggagatcca cgagacgggc aagctgccgg
      721 tcgtgaactt cgccgccggt gggatagcga ctccgagcga cgctgccctg atgatggcct
      781 tgggctgcga cgggatcttt gtgggctcgg gcatcttcaa gagcgacgat ccatccgggt
      841 tcgcccgggc catcgtcgaa gcaaccactt tctgggccga cgccgatgtt gttgccaaag
      901 tgtcgcgtgg cctcggtgaa gcgatggccg gtctcgagat cgacaagatc ccggcaggtg
      961 agaagatggc ggagcggggc tggtgagacc atgccggcaa ccggggtcct tgctcttcaa
     1021 ggagacttcc gcgagcacct gaccatgctt cgacaggttg ggaccgaccc gattccggtt
     1081 cggactccgg ccgaattggc cgaggtcgag gcgctggtga ttcccggagg ggagtcgacc
     1141 actatcggaa agctggccga tcgcttcggc ctcattgatc cgctgcgaac gcgcatcgag
     1201 gccggcatgc cggtgtatgg cacctgcgcg ggactgatct tcctctcggc cgcggtgacc
     1261 gagggtgagc aacacttgct cggggtcctc gacgcagtcg tccatcggaa cgcctttgga
     1321 cgtcaaaacg aatccttcga ggaggacctg gacatcgtcg gtctcgacac cccgtttcgg
     1381 gcggtcttca tcagggcgcc gtgggtggcc aagctgggcg acggagtcga ggtgctggct
     1441 acgattgatt cgcatccggt gatggtccgc caggggtcga tcctggcatc gtcgtttcac
     1501 cccgagttga ccgacgacac ccggatccat cgcatgctgg tcgatctgat ctagagagaa
     1561 gaaatgtccg gacactccaa atgggcgacc ctcaagcgga agaaggcggc gaccgattcc
     1621 aagcgcggga agctcttcgc caagttgatt cgggccatag aagtgtccgc ccggcaggga
     1681 ggcggggatg tcaacgccaa cgccacgctg gcggcggcag tagccaaagc ccgggccagc
     1741 gccgtcccgc tggacaacat cgagcgagcc atccagcggg ccacgggtga ggctgacac
//
LOCUS       DATITW010000039         1563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_442680, whole genome shotgun sequence.
ACCESSION   DATITW010000039 DATITW010000000
VERSION     DATITW010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442680"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1563
                     /gene="metG"
                     /locus_tag="VLS86_00450"
     CDS             <1..>1563
                     /gene="metG"
                     /locus_tag="VLS86_00450"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HSO48987.1"
                     /translation="TIATALPFTAFLIAAQVNPASRLSVAGYLVPVLAVTLAVIFLGE
                     TVTVAVVTGAVLIIGGVALTERASRHVPVAGIDTAGGLRGAVYPPPQMTRRILVAVAW
                     PYASGSLHLGHLGGAYLPSDIFARYHRIAGHDVLMVSGSDAHGTPITVRADEEGITPR
                     EVVDKYHAEILGYWEAVGISFDLFTTTMTENHHAVTQDVFLTLLEKGYLVKGTSTQFY
                     DPEAERFLPDRYVEGTCPHCGYTEARGDQCENCGRTLDATDLIDPRSRLSGATPVLRE
                     TEHYFFQLPAFAEQLKAWLESKTTWRRHVKNWALGMIEEGMPERAITRDIEWGVEVPV
                     DDLGPGKRIYVWFDAVIGYLSAAKEWAQRSGDPEAWRRWWEDPEAESYYFVGKDNIPF
                     HAVIWPAMLLGYGGLNLPTDVPANQYITFKGAKASKSKGVGRSIGWYAERLQPDALRY
                     AIASVLPEQNDTDLSDDEIIRRINDELVATWGNLVNRVLSLTSANFDGKVPDPGELTG
                     EDQGLLASVDKA"
BASE COUNT          285 a          535 c          473 g          270 t
ORIGIN      
        1 aacaatcgcc accgccctgc ccttcacggc tttcttgatc gcagcgcagg tgaatccggc
       61 ctctcgactc agtgtggccg gatacctggt gccggtcctg gcagtgaccc tcgcggtgat
      121 cttcctcgga gaaaccgtca ccgtcgcagt agtgaccggc gctgtcctca tcatcggcgg
      181 cgtggcactc accgagcgag ccagccgcca tgttccggtc gcgggcatcg acacagccgg
      241 tggattgcgt ggcgccgttt accctccgcc ccagatgact cgtcgcatcc tcgtcgccgt
      301 cgcctggccc tatgcctccg gttcacttca cctcggtcat ctcggcggcg cctatctccc
      361 ctccgacatt ttcgcccgct atcaccggat cgccggtcac gacgtcctga tggtctccgg
      421 ctccgacgcc cacggcaccc cgatcacggt tcgtgccgac gaggaaggca tcaccccgcg
      481 cgaggtggtc gacaagtacc acgccgagat cctcgggtac tgggaagcgg tggggatctc
      541 tttcgatctg ttcaccacca ccatgaccga aaaccaccat gccgtgaccc aggacgtgtt
      601 cctcaccctc ttggagaagg gctacctggt caagggcacc tcgacccagt tctacgaccc
      661 cgaggccgag cggttcctcc ctgaccgata cgtcgaaggg acctgccccc actgcgggta
      721 caccgaagcc cgcggcgacc agtgtgagaa ctgtggccgc accctcgatg ccacagacct
      781 gatcgacccc cgttcccggc tcagtggggc caccccggtc ctgcgcgaga ccgagcacta
      841 cttcttccag ctcccggcgt tcgccgagca gctcaaggcc tggctggaat cgaagaccac
      901 ctggcgtcgt cacgtcaaga actgggcttt ggggatgatc gaggagggga tgccggaacg
      961 agcgatcacc cgcgacatcg agtggggggt cgaggtgccc gtggatgatc tcgggcctgg
     1021 caaacggatc tacgtctggt tcgacgccgt catcggctac ctgtcagccg ccaaggagtg
     1081 ggcgcaacga agtggtgacc cggaggcctg gcgcaggtgg tgggaagacc cagaagcgga
     1141 gtcctactac ttcgtcggca aggacaacat cccgtttcac gcggtgatct ggccggcgat
     1201 gctcctcggc tacggagggc tcaacctccc caccgacgtc ccggcgaatc agtacatcac
     1261 gttcaaaggg gccaaggcgt caaaatcgaa gggcgtgggc cggtcgatcg gttggtacgc
     1321 agagcgactg caaccggacg cgctccgcta cgccatcgcg tcggtcctgc ccgagcagaa
     1381 cgacaccgac ctcagcgacg acgagatcat ccgcaggatc aacgacgaat tggtggcgac
     1441 ttggggcaac ctggtcaatc gggtcctctc gctcacctcg gccaacttcg acggcaaggt
     1501 gcccgacccc ggtgagctga ccggcgagga tcaggggctg ctggcaagcg tcgacaaagc
     1561 att
//
LOCUS       DATITW010000040         4448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_282351, whole genome shotgun sequence.
ACCESSION   DATITW010000040 DATITW010000000
VERSION     DATITW010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282351"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1405)
                     /locus_tag="VLS86_00455"
     CDS             complement(<1..1405)
                     /locus_tag="VLS86_00455"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HSO48988.1"
                     /translation="MSNHVCIHGHFYQPPRENPFTGEVAEQPTAAPWHDWNERITEEC
                     YAANARANILDDAGAVVRTVNTYERISFDFGPTLLSWLEESSPQTYQAIIGADDVSQQ
                     RFGGHGSAMAQAYNHTILPLTNRCDKVTQVRWGIADFEHRFGRRPEGIWLPETAVDTE
                     TLDLVAREGISFTVLSPYQAASAQRDDGTWDELEGSMVDTRVPYTVDLPEGRSISVFF
                     YDGPLSQEIAFNGLLNDGRDLARRLIEAGGAPGDRPALVHVATDGESYGHHHRHGEMA
                     LAVALDLIDADSEAHLVNYGEFLSLHPPSRKARIVEASSWSCAHGVERWRGDCGCSTG
                     QHPDWNQRWRGPLRQALDWLRDLLIEPFEVIGSRLFPDPWAARDAYIAVILGGPEEEF
                     MATHARPGLTAEEQATALGLLEIQHRSMLMYTSCGWFFDDISGLETVFVLRHAGRVAE
                     MAREVLGLDLEPGFLTRL"
     gene            complement(1437..3341)
                     /gene="glgB"
                     /locus_tag="VLS86_00460"
     CDS             complement(1437..3341)
                     /gene="glgB"
                     /locus_tag="VLS86_00460"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HSO48989.1"
                     /translation="MPRRVPESTSLLTDDDLHLWNEGTHYRLYQKLGSHPAETGTHFA
                     VWAPSADSVSVIGDWNGWDSGANPLRPRGVSGIWEGFIEGVTPTAAYKYRITNGDFVG
                     EKADPFGFHHETPPMTASKVWDLSYEWGDAEWMATRGPRNALDAPISVYEVHLGSWRR
                     AEDGRHLTYLELAEQLPTYLTRLGFTHVELMPVMEHPFYGSWGYQTTGYFAPTSRYGT
                     PQDFMALVDALHAAGIGVILDWVPSHFPEDAHGLGLFDGTHLYEHADPRLGFHPDWNT
                     YIYNYGRAEVPSFLISNALFWLDVYHVDGLRVDAVASMLYLDYSREEGEWVPNLHGGR
                     ENLEAIAFLRRLNEVVYLSFPDTQTMAEESTSWPMVSRPTFLGGLGFGLKWDMGWMHD
                     TLVHLSREPIHRLYHHHELTFRAVYAFSENFILPLSHDEVVYGKKAMVEKFPGDDWQK
                     FATLRLLLAYMYTQPGKKLLFMGVEIAQRSEWSHEGQLEWGLLDSEPHRGVQQVTTDL
                     NRVYADEPALHELDCDPRGFEWAVANDSANSVAAYFRRSEEGETLLVAINLTPVPRHM
                     YRIGVPAPGRWAEILNTDSEIYGGSGQGNLGGVDTGPLPVPHQGRPHSIDLTLPPLAV
                     VILKHEPTRS"
     gene            3564..4190
                     /locus_tag="VLS86_00465"
     CDS             3564..4190
                     /locus_tag="VLS86_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO48990.1"
                     /translation="MAYVADTSDRRRRRAVRAVSPFAALLVAALLIWQGSNAAFSATT
                     DNTSDAWATGNLALTNNGGGTVYSGTTAALFTESNLKPGDTGAKCITVQSSGSLPGSL
                     KLYRGTITGTNSVALAAVLDVTVDAVAVAAATNVQANCTGYTGGTSGAVFNGTLSGVP
                     STYAAAAGTAVAGGTQRVVYRIGWTINATAGNTVQSSSAQANLTWEIN"
BASE COUNT          788 a         1434 c         1470 g          756 t
ORIGIN      
        1 cgagcctggt gaggaacccc ggctccaggt cgagaccgag cacctcccga gccatctcgg
       61 cgacccggcc cgcatgacgc aacacgaaca ctgtctccaa tcccgagatg tcgtcgaaga
      121 accagccgca ggacgtgtac atcagcatcg accgatgctg gatctcgagc agccccaggg
      181 ccgtcgcctg ctcctcggcg gtgagcccgg gtcgggcgtg ggtggccatg aactcctcct
      241 cgggcccgcc cagaatcacc gcgatgtagg cgtccctggc cgcccacggg tcggggaaca
      301 gccgggagcc gatgacctcg aagggctcga tcagcaggtc gcgcaaccag tcgagagcct
      361 ggcgcagcgg gccgcgccat cgttggttcc agtccgggtg ctgtccggtg gagcagccac
      421 aatcccccct ccatcgctcc accccgtggg cacagctcca tgagctggcc tcgacgatcc
      481 tcgcctttcg cgacggcggg tggagggaga ggaactctcc gtagttgacg agatgggctt
      541 cggagtcggc gtcgatcaga tccagggcca cggcgagcgc catctcgcca tgacggtgat
      601 gatggccgta gctctcccca tcggtggcga cgtggaccag ggccgggcga tcaccgggcg
      661 caccacccgc ctcgatcagc cggcgagcca ggtcacggcc gtcgttgagc aggccgttga
      721 aggcaatctc ctgggagaga gggccgtcgt agaagaagac cgagatgctg cgaccttcgg
      781 ggaggtcgac ggtgtacggg acccgggtgt cgaccatcga cccttccagc tcgtcccagg
      841 tgccgtcatc cctctgcgcc gaggctgcct ggtaggggga gagcacggtg aacgagatcc
      901 cctcccgggc gaccaggtcg agggtttcgg tgtcgacggc ggtctccggg agccagatcc
      961 cctccggacg acggccgaac ctgtgctcga agtcggcgat tccccaccgc acctgggtca
     1021 ccttgtcgca ccggttggtg agggggagga tggtgtggtt gtaggcctga gccatcgccg
     1081 agccatggcc accgaagcgc tgctgcgaga cgtcgtcggc cccgatgatc gcctggtagg
     1141 tctggggaga gctctcctcc agccagctga gcagggtggg gccgaagtcg aagctgattc
     1201 gctcataggt gttgaccgtg cgcacgaccg caccggcgtc gtcgaggatg ttcgccctgg
     1261 cgttggccgc gtagcactcc tcggtgattc gctcgttcca gtcgtgccat ggggcggcag
     1321 tgggctgctc ggccacctcg ccggtgaatg ggttctcccg aggcggctga tagaagtgcc
     1381 cgtggatgca gacgtgattg ctcatcggaa gacgtcgggg ctcggcaatt cccggctcag
     1441 gaccgcgtcg gctcgtgctt gaggatgacc acggcgaggg gaggcagggt caggtcgatg
     1501 ctgtggggcc gaccctgatg gggaacgggc agcgggccgg tgtccacacc accgaggttt
     1561 ccctggccac tgccaccgta gatctccgag tcggtgttga ggatctcggc ccatcgaccg
     1621 ggagcgggga caccgatccg gtacatgtgc cgtggcaccg gcgtcaggtt gatcgcaacc
     1681 agcagcgtct ctccttcctc ggaccttcga aagtaggcgg ccacgctatt cgccgagtcg
     1741 ttggcgaccg cccattcgaa tccgcgtggg tcgcagtcga gctcgtggag ggccggctca
     1801 tcggcataga cgcggttgag gtcggtcgtc acctgttgca ccccccgatg cggctcgctg
     1861 tcgaggagac cccattcgag ttggccctcg tgggaccact cggagcgctg ggcgatctcc
     1921 acccccatga acaacagctt cttgcctggc tgggtgtaca tgtaggcgag aagcagccgg
     1981 agcgtggcga acttctgcca gtcgtccccg gggaacttct cgaccattgc cttcttcccg
     2041 tagaccacct cgtcgtggga aagggggagg atgaagttct cgctgaaggc gtacaccgcg
     2101 cgaaaggtca gctcgtgatg gtgatagaga cgatggatcg gctcccgcga gagatggacc
     2161 agggtgtcgt gcatccaccc catgtcccac ttcaacccga agccgaggcc accgaggaag
     2221 gtcggtctcg acaccatcgg ccatgaggtg gactcctcgg ccatggtctg ggtgtcggga
     2281 aagctgaggt agacgacctc gtttagccgt ctgagaaagg cgatcgcctc caggttctcc
     2341 cgccctccgt ggagattggg gacccactcc ccttcttcac gcgagtagtc gaggtagagc
     2401 atggaagcca cggcatcgac ccgcagcccg tcgacgtgat agacgtcgag ccagaagagg
     2461 gcattgctga tcaggaacga agggacctcg gcccgcccgt agttgtagat gtaggtgttc
     2521 caatcaggat gaaacccgag ccgggggtcg gcatgctcgt agagatgggt gccgtcgaac
     2581 agacccagac cgtgggcgtc ctcgggaaaa tgcgaaggga cccagtcgag gatcacgccg
     2641 atacccgccg catgcagggc gtcgacgagt gccatgaagt cctgcggggt gccatagcga
     2701 ctggtcgggg cgaagtagcc ggtggtctga tagccccatg agccgtaaaa ggggtgctcc
     2761 atcaccggca tcagctcgac atgggtgaac ccgagccggg tcagatacgt ggggagctgt
     2821 tcggcgagct cgagataggt cagatggcgg ccgtcttcgg cccggcgcca tgacccgaga
     2881 tggacctcat agacactgat cggggcgtcc agcgcattgc ggggccccct ggtcgccatc
     2941 cactcggcgt cgccccattc gtaggagaga tcccagacct tggatgcggt cattggcggg
     3001 gtctcgtggt gaaagccgaa cggatcagcc ttctccccga cgaagtcccc gttggtgatc
     3061 cggtatttgt acgccgcggt cggcgtcacc ccctcgatga atccctccca gatcccggag
     3121 acaccccggg gtcgcagtgg attggcgccc gaatcccagc cgttccagtc gccgatgacc
     3181 gaaacgctgt cggcactcgg ggcccagacc gcaaagtggg tcccggtctc ggcggggtgg
     3241 gagcccaact tctggtacaa gcggtagtgg gtcccctcgt tccagagatg caggtcgtcg
     3301 tcggtgagaa gactcgtaga ttcagggacg cggcgcggca agctctcttc ctggccgatg
     3361 gcccggcgag tgtatccgag ccgtctaggc cctccggccc agagcggtcc tccgcaagtc
     3421 gtcaatggga agaaacccct tgggtccaca ggcctattcg atgactagtc accatgtcgt
     3481 ttgtgaggac tgtcctcgtc cgctgccccc cgaaatgtcg atgaatagag gacagaccga
     3541 ccggacacga aaggcttcag accatggcat acgttgcaga caccagcgac cgacgacgga
     3601 gaagggccgt tcgggccgtc tccccgttcg ccgctctcct cgttgccgcc cttctcatct
     3661 ggcagggcag caacgcagca ttcagcgcca cgaccgacaa caccagcgat gcgtgggcaa
     3721 ccgggaacct cgccctgacc aacaacggtg gtggcacggt ctactcgggc accacggcgg
     3781 cactcttcac cgagagcaac ctcaagcccg gcgacaccgg ggccaagtgc atcaccgttc
     3841 agtccagtgg ctcgctgccg ggcagcctca agctgtaccg gggaacgatc accggcacca
     3901 acagcgtcgc cctggctgca gtgttggacg tcaccgtgga cgcggttgcg gttgccgccg
     3961 ccaccaacgt ccaggccaac tgcaccgggt acaccggcgg cacctcagga gcggtcttca
     4021 acggcacact gagcggcgtg ccttcgacct acgcggcggc cgcgggcacc gcggtggccg
     4081 gcgggaccca gcgtgtggtc taccggatcg gctggaccat caacgccacc gccggtaaca
     4141 ccgtgcagag ctcctcggcg caggccaacc tgacctggga gatcaactag ggacgattcg
     4201 catccgatct gagacgccgg ccctggcacg ggagtccccg ggccggggcc ggcgtcgtat
     4261 cagagggaaa gtggagcccc atcgagatga ctgccataac cgaaccgatc cggagcgaag
     4321 tccacatcca ccagccctac tcccggctgc gtctggctgc cggtttcatc ggccgctcct
     4381 ggctctggtt cctcgcagcc tgtgtcctgg tgacatttat ccccctgctc ttcggatggc
     4441 gaccgttc
//
LOCUS       DATITW010000041         1939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_763209, whole genome shotgun sequence.
ACCESSION   DATITW010000041 DATITW010000000
VERSION     DATITW010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763209"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VLS86_00470"
     CDS             complement(<1..192)
                     /locus_tag="VLS86_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO48991.1"
                     /translation="MRVDPATTRTLIHRDPQVQEVLDIWAARFLAGQIPLGDLTTTID
                     RIDNWSDWGPEWMATARVHE"
     gene            complement(189..1247)
                     /locus_tag="VLS86_00475"
     CDS             complement(189..1247)
                     /locus_tag="VLS86_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSO48992.1"
                     /translation="MSEPVKLASIGLGRWAKVLARAAQRGSVIELASCFSRSPEKRAA
                     FQDEFGIPRGASTLDEVLGDPEIEGVLVTTPNDTHKSVILECLDAGKAVYTDKPIAHT
                     MEDAGEIARAAAASGLAFAVGHSARRLGGHRAMKQWVETGKIGQVSLAEANFSNERGL
                     ELTTDTWRWFADKSPGGAMIQLGVHHADNLQYLLGPVAAVSGHARKLYTKAEVPDAIM
                     SILEFESGPLGYLGTGWASPGVYQLRLQGTEANLFYDLDFNNWDESHLVDSKSTLVSQ
                     AYRESVRPPVDMPHTDMFREQLDEFALAIRGEAAIEVGALEAIRALAVVHASLESSER
                     NGEAVAVAPMVEKAGALQ"
     gene            complement(1240..1629)
                     /locus_tag="VLS86_00480"
     CDS             complement(1240..1629)
                     /locus_tag="VLS86_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSO48993.1"
                     /translation="MTVVGAEELEFREFPGRRTSDPFRHAGQGESTVRQVVIEHVASR
                     SPHLHPKSEEIVYVVEGRGRVWLNGVFHSVGPGSWYRIPVGTPHATLADAGERMSLVC
                     FFPDDDFASNIEELDLLLEPDKEERDE"
     gene            complement(1626..>1939)
                     /locus_tag="VLS86_00485"
     CDS             complement(1626..>1939)
                     /locus_tag="VLS86_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HSO48994.1"
                     /translation="AGGELTHGGGIETLAVLAHDPELARVDQVKEPTRPEGAEELDAM
                     RRSREVYGYITDVTEIADDGWYGNPAWADEERAATFATSVANEIAVTVESILKMRGRG
                     S"
BASE COUNT          307 a          636 c          634 g          362 t
ORIGIN      
        1 ctcatgtacc cgggctgtcg ccatccactc ggggccccaa tcagaccagt tgtcgatgcg
       61 gtcgatggtc gtggtcaggt ctccgagcgg gatctgaccc gccagaaacc gggcggccca
      121 gatgtcgagc acctcttgga cctgtgggtc ccgatggatg agggtgcgcg tcgtggccgg
      181 atcgaccctc actggagggc tcccgccttc tccaccatgg gcgcgacagc gaccgcctca
      241 ccgttgcgct ccgaggactc cagtgaagcg tggaccaccg ccagggcgcg gatcgcctcc
      301 aaggccccaa cctcgatcgc ggcttcgccc ctgatggcga gggcgaactc gtcgagttgc
      361 tctcggaaca tgtcggtgtg aggcatgtcg acgggcggtc tgaccgactc ccgataggcc
      421 tggctgacca gggtgctctt cgagtcgacc aggtgcgact cgtcccagtt gttgaagtcg
      481 aggtcgtaga acaggttcgc ctcggtcccc tggagacgga gctggtagac gccgggtgag
      541 gcccatcccg tgcccaggta cccgagcggg cccgactcga actcgaggat cgacatgatg
      601 gcgtcgggga cttccgcctt ggtatagagc tttcgggcgt gaccggacac cgcggccacc
      661 gggccgagca ggtactggag attgtcggcg tggtgtaccc cgagttggat catcgcccca
      721 ccggggctct tgtcggcgaa ccagcgccag gtgtcagtgg tcagctcgag cccgcgttcg
      781 ttggagaagt tcgcctccgc cagggatacc tgaccgatct tgccggtctc gacccactgc
      841 ttcatggcgc gatgtccacc gagccttcga gcgctgtggc ccacggcgaa agcgagaccc
      901 gaagcggcgg ccgcccgtgc gatctccccg gcgtcctcca tggtgtgcgc gatcggcttg
      961 tcggtgtaga ccgccttgcc cgcgtcgagg cattcgagaa tgacgctctt gtgggtgtcg
     1021 tttggcgtgg taaccagcac cccctcgatc tcagggtcgc ccagtacctc atcgagcgtc
     1081 gaagcgccac ggggaatgcc gaactcgtcc tggaaggcgg cgcgcttctc gggagaacgc
     1141 gagaagcagc tggccaactc gatgaccgag ccccgctggg ctgctcgtgc cagcaccttt
     1201 gcccagcggc ccagtccgat cgatgcgagc ttgacgggtt cactcatctc tctcctcctt
     1261 gtcgggctcg agaagcaggt cgagttcctc gatgttgctc gcaaaatcgt cgtcggggaa
     1321 gaagcagacg agtgacatcc gctcgccggc gtcggcgagg gtggcgtggg gagtccccac
     1381 cgggatgcga taccaggagc ccgggcccac cgaatggaag accccgttca gccaaactcg
     1441 tcccctgcct tccaccacgt agacgatctc ctcgctcttc gggtggaggt gaggggatcg
     1501 gctggctacg tgctcgatga ccacctgtcg gaccgtggac tcaccttggc cggcgtgtcg
     1561 gaaagggtcg gatgtcctcc tgccgggaaa ctctcggaac tcgagctctt cagcgccgac
     1621 gaccgtcatg atccacgccc tctcatcttc aggatcgact cgaccgtgac ggcgatctcg
     1681 tttgccaccg aggtggcgaa ggtggcagca cgttcctcgt cggcccatgc cgggttcccg
     1741 taccagccgt cgtcggcgat ctcggtgacg tcggttatgt agccataaac ctcgcgggag
     1801 cggcgcatgg cgtctagctc ctcggctcct tcaggcctcg tcggctcttt gacctggtcg
     1861 acccgggcga gttcgggatc gtgggcgagg acggcaagag tctcgatccc gccgccatga
     1921 gtgagctccc ctccggccg
//
LOCUS       DATITW010000042         1597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_122309, whole genome shotgun sequence.
ACCESSION   DATITW010000042 DATITW010000000
VERSION     DATITW010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122309"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..636
                     /locus_tag="VLS86_00490"
     CDS             283..636
                     /locus_tag="VLS86_00490"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSO48995.1"
                     /translation="MASPPEFGGPGAVWSPEHLFVASVSACLMTTFRSIAEMSGLEVL
                     AYSDSATGTLVRGDDRLFKMTEITLRPHVEIADPAMADRALRLLAKAEKVCLISRSIT
                     STVHIEPSVDIAVAA"
     gene            complement(637..>1597)
                     /locus_tag="VLS86_00495"
     CDS             complement(637..>1597)
                     /locus_tag="VLS86_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HSO48996.1"
                     /translation="PVMPSASPLTGDIDHILALVEAGAPAIVLPSLFEEQIEHDAMAV
                     HFGIELGAEGFAEAPSGYLPSLNSYNTGPERYLDLVRRARSEAGVPVIASLNGVSSGG
                     WTLYARVVADEGIDALELNIYQIAGDVFSKSSDVEKSYLRLVETVRAAVEVPLAVKVG
                     PYFSAMASMARRLGEAGADGLVIFNRFYQPDIDLSTLEVTPNLQLSTSSELRLVLRWL
                     AILSGRVDVDLAATTGVHVGEDAVKAVLAGADVVMMASALLRAGPKALTTVQVGLQTW
                     LEENDYESVAQARGSLRQRAVSDPSAFERSNYMKTLTSYVPSW"
BASE COUNT          289 a          528 c          521 g          259 t
ORIGIN      
        1 aagatgggga tgagccgccg cagcgaggct gcggcctacg tggcccgtct cgaaggggag
       61 cacgagggcg ccgacgcgcc ccagggatgg ggcgagggct gatccaagcc cgacgtcttt
      121 agtgcccaaa gaccacagat caagggcggt tcgccccggg catgtttccg gatcctgctg
      181 tatccaggag taatggaaca ccagaaatac gacgtcgaca tcctgggctc agggctcaag
      241 acgggacgtc tccggtcgcc gcaaggcctc cccgagctgt aggtggcctc cccgcccgag
      301 tttggcgggc cgggcgcggt gtggagcccc gagcacttgt tcgtggcttc ggtgtccgcc
      361 tgtctgatga caacgttcag gtcgatcgcc gagatgtcgg ggctggaagt gctcgcctac
      421 tcggactcgg ccaccgggac cctggtccgt ggcgacgacc gactgttcaa gatgaccgag
      481 atcacgctcc gtccccacgt cgagatcgcc gacccggcga tggccgatcg ggcgctacgg
      541 ctcctggcca aagcggagaa ggtgtgcctc atcagcagat cgatcacctc gacggttcac
      601 atcgagccgt cggtcgacat cgccgtggcc gcctgatcac cagctcggca cgtagctggt
      661 cagcgtcttc atgtagttgg accgctcaaa ggctgatggg tccgagaccg cgcgctgacg
      721 gagactcccc cgcgcctggg ccaccgactc atagtcgttc tcttccagcc aggtctggag
      781 accgacttgc actgtggtta gggccttggg cccggctcgg agcagggcgg aggccatcat
      841 cacgacgtcg gcgccggcga ggacagcctt gaccgcgtcc tccccgacgt ggacgccggt
      901 ggtggcagcc agatccacgt cgacgcggcc actgagaatt gccagccaac gcagcaccag
      961 acgaagctcg cttgaagtgg acagctgcaa attgggggtc acctcgaggg tgctgaggtc
     1021 gatgtccggc tggtagaagc gattgaagat caccagcccg tcggcacccg cctcgccgag
     1081 ccttcgcgcc atggaggcca tggcggagaa gtaaggcccg accttgaccg cgagcgggac
     1141 ctcgacggct gcccgcaccg tctccaccag acggaggtag ctcttctcca cgtccgagga
     1201 cttggagaat acgtcccccg cgatctggta gatgttcaac tcgagcgcat caatgccctc
     1261 gtctgcgacc actctcgcat acagcgtcca cccccccgag gacacgccgt tcaggctggc
     1321 gatgactggc accccggctt cggagcgagc ccggcgcacc agatccagat agcgctcggg
     1381 gccggtgttg tagctgttca ggctggggag atacccgctg ggcgcctcgg caaagccctc
     1441 ggcgcccagc tcgatcccga aatggacagc catcgcatcg tgctcgatct gctcctcgaa
     1501 gagactggga agcacgatcg ccggcgcccc cgcctccacc agagccagga tgtgatcgat
     1561 gtcgccggtc aacggggacg ccgaaggcat gaccggg
//
LOCUS       DATITW010000043         1954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_683229, whole genome shotgun sequence.
ACCESSION   DATITW010000043 DATITW010000000
VERSION     DATITW010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683229"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /gene="rplI"
                     /locus_tag="VLS86_00500"
     CDS             <1..340
                     /gene="rplI"
                     /locus_tag="VLS86_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HSO48997.1"
                     /translation="KASEGSMAQAEATREARIESEARAREQAENLATQLVGTRVVLAA
                     QAGDEGKLYGSISVHDVVEGIKRFTGLELERDTVVLPAPIKAIGLHEIQIKLHPEVEF
                     PLILDVIPAS"
     gene            599..1951
                     /gene="dnaB"
                     /locus_tag="VLS86_00505"
     CDS             599..1951
                     /gene="dnaB"
                     /locus_tag="VLS86_00505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HSO48998.1"
                     /translation="MSGLTEQTEMRPGYRSPPHSREAEESVIGAVLLSEDAVNEVMDR
                     LHPEDFYIPAHQAIFESMRQLFDGNQAIDAVTVSEELRRRGELEKVGGVNYLTRLVDI
                     VPSTSNILYYAGIVEEHGKRRELIRAGATVTEVAFNIDEEIAIVLDRAEQTVLGVAER
                     RSSQTLLEVGPLFSEVLEHIEALEQLGSEINGLATGFVDLDSKLAGIQPANLVVIAGR
                     PGMGKSAIALNIATNVAGKGEPVAIFSLEMSKEEVVQRILSSVGQVDSKKLRNGQLGP
                     LWQRVVDAAGKMYKAPIFIDDSSVVTVTDIRAKCRRLKRKRGLSLVIVDYLQLLQAST
                     RENRQQEISEITRNLKNLAKELAVPIIAVSQLNRSVESRENRRPRLSDLRESGAIEQD
                     SDVVMFVYRHEYYHPEDQEKQGIAELIIEKHRSGATGTVEMTFQPEFTRFANLGRDVS
                     "
BASE COUNT          373 a          608 c          625 g          348 t
ORIGIN      
        1 gaaggccagc gagggctcga tggcccaggc cgaggccacc cgtgaggctc gtatcgaaag
       61 tgaggcccgg gcacgggagc aggcggagaa tctcgccacc cagctcgtgg gcacgagggt
      121 ggtgctcgcc gcccaagcgg gtgacgaggg caagctctac ggctcgatct cggtccacga
      181 cgtggtcgag gggatcaaac ggttcaccgg cctcgagctg gagcgggaca cggtcgtgct
      241 tcccgccccg atcaaggcga tcgggctcca cgagatccag atcaaactgc atcccgaggt
      301 cgagttcccc ctcatcctcg acgtcatccc cgccagctga attcctaatg caggacgtca
      361 cggatacgtg acgccctgca taaagaaccc cggttccggt gaactagcgt cagatttcgc
      421 gtgtttacgc accgatttcg cgtcttttga cagtgtttcg cggccttttg gaatgcgacc
      481 cggctgtggt ctatgttcca ttcgcttccc cagaagctgg ccgatcttcc cctaacccgt
      541 cagggtttga cgcgtcggtt cgcgaaaagt gtcacacggc gttggtagaa gagggtagat
      601 gagcggcttg accgaacaga ccgagatgag gcccgggtac cggagcccac cccactcccg
      661 tgaggccgag gagtctgtca tcggcgcggt gctgctatcc gaggacgcgg tcaacgaggt
      721 gatggaccgg cttcatcccg aggacttcta catcccggct catcaagcca tcttcgagtc
      781 gatgcgtcag ttgttcgacg gcaaccaggc gatcgacgcg gtcaccgtct ccgaggagct
      841 tcgtcgccgc ggcgagctgg agaaggtcgg gggggtcaac tacctgaccc ggctggtgga
      901 catcgtcccc tccacctcca acatcctcta ctacgccggg atcgtcgagg agcacggcaa
      961 gcggcgggag ctgatccggg cgggggccac ggtcaccgag gtcgccttca acatcgacga
     1021 ggagatcgcg atcgtcctcg accgtgccga gcagacggtg ctgggtgtcg ccgagcgacg
     1081 cagctcacag accctgctcg aggtcggccc tttgttcagc gaagtcctgg agcacatcga
     1141 ggctctcgaa cagctcggct cggagatcaa cgggttggcg accgggttcg tcgacctcga
     1201 ctccaagctc gctgggatcc aacctgccaa cttggtggtg atcgccggcc ggccgggcat
     1261 gggcaagtcg gccatcgctt tgaacatcgc aaccaacgtt gccggcaagg gcgagccggt
     1321 ggcgatcttc tcgttggaga tgtccaagga ggaggtggtg cagaggatcc tgtcttcggt
     1381 gggccaggtc gattccaaga agctccgcaa cggccaactc gggccgctct ggcaacgggt
     1441 ggtcgacgcc gcggggaaga tgtacaaggc gccgatcttc atcgacgact cctcggtggt
     1501 cacggtgacc gacatccggg ccaagtgccg gcgtctcaag cgaaagcgag ggctctccct
     1561 ggtcatcgtc gactatctcc aattgctcca ggcttcgacc cgggagaatc gccagcagga
     1621 gatctcggag atcacccgaa acctgaagaa cctggccaag gagctagcgg tcccgatcat
     1681 cgccgtctcc cagctcaatc gctcggtgga gtcacgtgag aaccgacgtc ctcgtctctc
     1741 cgacctgcgc gagagtggcg ccatcgagca ggactcggat gtggtgatgt tcgtctaccg
     1801 ccacgagtac taccaccccg aggatcagga gaagcagggg atcgccgagc tgatcatcga
     1861 gaagcatcgc tccggtgcca ccgggaccgt ggagatgacc ttccagcccg agttcacccg
     1921 cttcgccaat ctgggccggg atgtgagctg agat
//
LOCUS       DATITW010000044         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_162363, whole genome shotgun sequence.
ACCESSION   DATITW010000044 DATITW010000000
VERSION     DATITW010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162363"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1368
                     /locus_tag="VLS86_00510"
                     /pseudo
     CDS             190..1368
                     /locus_tag="VLS86_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
     gene            1368..>2017
                     /locus_tag="VLS86_00515"
     CDS             1368..>2017
                     /locus_tag="VLS86_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HSO48999.1"
                     /translation="MAVLSRLREVAVDRRNNRSDQVLFGTMLALSGFGLLMIYSATRS
                     AGSVSMERQMIFVAAGLTIYLIVSNIDYREYKAIIPWISAVILVGLIAVYAFPPVNGA
                     RRWIPLGFFKLQPAEFAKVAVIVLLAVILSPHTRDEIGRRNLRWTHIFQALTVVAIPA
                     VLIYFEPDLGTTMVFGFILFAMLFTAGATWRQLVALLSATVLGGFVIWQQGWLAEY"
BASE COUNT          359 a          635 c          635 g          388 t
ORIGIN      
        1 ggtctcgtcc agtatcaggt gcaacggggt cgaatcgtcg accagaagcc gccgatcgag
       61 cccgaaccgg gtaactcggt ggtgctcaac atcgatttcg agctccagga gttggtcgag
      121 ctggccctcg aggagggggt cgctctttcc aattcggtca aggaacaggc gcgcgacgcc
      181 ggggacgacg tgttcagcga gactcagcgg gccgccgcgg tcgtcctcga tgccaagacc
      241 tttgatgtca tggccctggc ttccttcccc gatttcgacc cccagttgtt cgtggccggg
      301 atcgatgccg ccaccttcga agggctgacc gatagcaagg cattcaacaa tctggcgatc
      361 agcggtctct atccgccgtc ttcgacgttc aaggcgatta cctacacggt gacgtaggaa
      421 gaggacctcc cgttggaccc cgacctcgaa ggggtggatg tcgccaatca gaccgtccac
      481 tgcgacggcg agctcatcct ctccgagctc gaagacggct cgcagccacc aaagaccgac
      541 tggtactacc cggacaacaa gggttggctc gacatccacg gagcgttgga gcaatcctgc
      601 aacatctact tctggtcggt ggcgatgggc acctggcagg cgttcaaggg caccccacgg
      661 gagaacgttc tccaggattg ggcgaaatcc gttggatatg ggtcggcgac ggggatcgat
      721 ctcagcgccg aggccgaagg caccgtcccc acccgggagt tgttcgagga gtgggccgtc
      781 taccagctgg agaacccgga ccagcctccc ctgctcgagc cgaatcgact cgaactggcc
      841 agcccctggc tgggtggtga cctggtggac atggcgatcg gtcagggagc gttcacggca
      901 accccactcc aggtcgcggt ctcctatgcg gctctggtca acgggggcca gatcatggag
      961 ccacgcgtcg tcaaccggat cgtggacgcg gaggggaccg tcgttttcga gccggagtcg
     1021 cccgtggtcg gcaccgttga catcgacccc gagacccgct cccagcttct gaccgacttg
     1081 aatcgggttg tgaccagtgg caccgccgcc accgcctttg ccgatttcgg gcctggcctg
     1141 gagaacgtcg gggggaagac cgggaccggg cagacctcga agaatcggga caatcacgcc
     1201 tggttcgtgg gggtggcgcc aatcgatgat ccccggtaca tcgtggtgat cctcatcgac
     1261 gaaggtgggt ccggaggacg ggtcgcggcc ccggtcggcc gtcacatcat gcaatacctg
     1321 atgggcaacg agccgacccc gatcgtcgaa ggcgcccagg cggactgatg gcagtgctca
     1381 gccgtctccg cgaggtggcc gtcgatcggc gtaacaaccg gtcggaccag gtcctgttcg
     1441 ggacgatgtt ggccctgtcc gggtttgggc tgttgatgat ctactcggcc accaggagcg
     1501 cagggtcggt ttcgatggaa cggcagatga tcttcgtcgc cgccgggctc accatctatc
     1561 tgatcgtctc caacatcgac tatcgggagt acaaggcgat cattccctgg atctcggcgg
     1621 tgatcctggt cggattgata gcggtctacg ccttcccacc ggttaacgga gcgcgacgct
     1681 ggatccccct cggcttcttc aagctgcaac ccgcggaatt cgccaaggtc gccgtgatcg
     1741 tgctcttggc ggtgatcctc tcaccccata cccgtgacga gatcggacgt cgcaatctcc
     1801 gctggaccca catcttccag gcgctgacag tggtcgccat ccctgccgtt ctcatctact
     1861 tcgagccgga cctgggcacc acgatggtgt tcgggttcat acttttcgcc atgttgttca
     1921 ccgccggcgc cacctggcgc cagctggtcg cattgctctc cgccacagtg ctcggagggt
     1981 tcgtcatttg gcaacagggc tggctggccg aatacca
//
LOCUS       DATITW010000045         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_402669, whole genome shotgun sequence.
ACCESSION   DATITW010000045 DATITW010000000
VERSION     DATITW010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402669"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VLS86_00520"
     CDS             complement(<1..342)
                     /locus_tag="VLS86_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014058685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSO49000.1"
                     /translation="MTASQSLSIGEVINLLRDDFPDVSVSKIRFLESQGLIDPGRSDS
                     GYRQFDQTDLARLKFILQQQRDHFLPLKVIKSKLTLWERGEDLSTKISQPAAKIESKG
                     EPLSREDLLRRS"
     gene            complement(332..775)
                     /locus_tag="VLS86_00525"
     CDS             complement(332..775)
                     /locus_tag="VLS86_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HSO49001.1"
                     /translation="MTDEREREEGQPEPQVEVESDPTVVYVPIQDRAEHPMTAEEASN
                     LEGIWGYALVVHRGPRTGMTYVLPAGNTTVGRHPESDIFLNDVTVSRQHCRFVITDAG
                     LMVEDSGSTNGTYVNDARVDEAMLVAGDEVLVGRFHFVVAHGDDR"
     gene            complement(820..1200)
                     /gene="gcvH"
                     /locus_tag="VLS86_00530"
     CDS             complement(820..1200)
                     /gene="gcvH"
                     /locus_tag="VLS86_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HSO49002.1"
                     /translation="MQVPDDLLYTEEHEWIRKVEGSLVQIGITDYAQDQLGDIVFVEL
                     PEVGATVAKDDILVEVESTKSVGEVYAPFAGTVTAINQAVADNPELVNQSPYDQAWLV
                     TLSLDGEIPSDGLLDAAAYRQLTE"
     gene            complement(1200..1796)
                     /locus_tag="VLS86_00535"
     CDS             complement(1200..1796)
                     /locus_tag="VLS86_00535"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSO49003.1"
                     /translation="MSDGVASNRILTIPNLVSFIRLLGIPVFWWALLVQDDVALAAWL
                     VFGIGWTDWIDGYLARRLNQVSELGKALDPIADRLLIASALIGGLIAGVLPLWFGWGL
                     IAREVLVGIVALNLAVRGGGTIEVRYLGKLATFLLYGAIPAFYLAEAGVAEWFMWPAA
                     YINGIVGLALYWYVAFFYIGDSRRKLAGLESTPHPEEV"
     gene            complement(1818..>2146)
                     /locus_tag="VLS86_00540"
     CDS             complement(1818..>2146)
                     /locus_tag="VLS86_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49004.1"
                     /translation="GWTLGNPALPDFYRLFFASDGKFNSTGYASPQFEALLARYEQAT
                     DVDAAKKALWDMEKALAIDLPYLALYHPVIIEAYRSDRLGFGETATLGGIQGRLGGIG
                     DLEPVE"
BASE COUNT          409 a          673 c          680 g          384 t
ORIGIN      
        1 ggatcggcgg agcaggtcct cgcggctcaa cggctcgccc ttggactcga tcttggctgc
       61 aggctgggag atcttggtcg acagatcctc cccccgctcc cagagcgtga gcttcgactt
      121 gatcaccttc agtgggagaa aatggtccct ctgctgctgc aggatgaatt tgagccgcgc
      181 caggtcggtc tggtcgaact gccggtagcc gctgtcggag cgcccggggt cgatcagacc
      241 ttgagactcg agaaaacgga tcttggacac cgaaacgtcg ggaaaatcgt cgcggaggag
      301 gttgatgacc tctccgatgc tgagcgattg gctagcggtc atcgccgtgg gcgaccacga
      361 agtggaatct ccccaccaac acctcgtctc cggccacgag catcgcctcg tcgaccctgg
      421 cgtcgttgac ataggttccg ttggtcgaac cactgtcctc gaccatcagc ccggcgtcgg
      481 tgatgacaaa ccggcaatgc tgtcgggaga cggtgacgtc gttgagaaag atgtcgctct
      541 ccgggtgacg gccgaccgtg gtgttgcccg ccggcaggac gtaggtcatc cccgtcctcg
      601 ggccgcgatg aacgaccagg gcatatcccc aaatcccctc caggttggag gcctcctcgg
      661 cggtcatcgg gtgctcggcg cgatcctgga tcggcacgta gaccaccgtt gggtcgcttt
      721 cgacctcaac ttgaggttcg ggctggcctt cctcgcgctc tctctcgtcg gtcatctggt
      781 gtgatgatag gtaattccgg catatgagcc ggccaccctc tactcggtga gctggcggta
      841 ggcggcggcg tcgagaaggc cgtctgacgg aatctcaccg tcgagggaca gggtgaccag
      901 ccacgcctgg tcgtacgggc tctggttgac caactcggga ttgtcggcga cggcctggtt
      961 gatcgcggtc acggtgccgg cgaaaggggc gtacacctcc cccaccgact tggtgctctc
     1021 cacctccacc aggatgtcgt ccttggccac cgtggcgccg acctcgggga gctcgacgaa
     1081 cacgatgtcg cccagttgat cctgggcgta gtccgtgatc ccgatctgga ccaaagagcc
     1141 ttctaccttg cggatccact catgttcctc ggtgtagaga agatcgtccg gtacctgcat
     1201 caaacctcct cggggtgcgg cgtggactct aaccctgcga gctttcggcg gctgtcgccg
     1261 atatagaaga aagcgacgta ccaatagagg gccaggccca cgatgccgtt gatgtatgcc
     1321 gcgggccaca tgaaccactc ggcgaccccg gcctcggcga gatagaaggc cgggatggcc
     1381 ccgtagagca agaaggtggc gagtttgccg aggtatcgca cttcgatcgt cccccctccc
     1441 ctgaccgcca ggttgagggc gacgatccca accaacacct ccctggcgat cagaccccag
     1501 ccgaaccaga ggggaagcac tcctgcgatc agcccgccga tcagggcgga ggcaatcaaa
     1561 aggcggtccg cgatcgggtc gagcgccttg cccagctcgc tgacctgatt cagcctccgg
     1621 gcgagatagc cgtctatcca gtcggtccaa ccgatgccga aaaccagcca ggcagccagc
     1681 gccacatcgt cctgcaccag aagcgcccac cagaaaacgg ggatcccgag gagtcggatg
     1741 aaggagacga gattgggaat ggtcaggatg cggttgctag ccacgccgtc gctcacgagg
     1801 gcaggctaac ggcggctcta ctcgaccggt tccaggtctc cgataccgcc aagacggccc
     1861 tggatgccgc ccaaggtcgc ggtctcacca aacccgagtc gatcggagcg gtaggcctcg
     1921 atgatcacgg ggtgatagag ggccagatag ggaagatcga tcgccagagc tttctccatg
     1981 tcccagagag ccttcttggc cgcatcgacg tccgtagcct gctcatagcg ggcgagaagc
     2041 gcctcgaact gcgggctggc gtacccggtc gagttgaact tcccgtcgga ggcgaagaag
     2101 agccgataga agtcggggag ggcagggttg cccagcgtcc aaccga
//
LOCUS       DATITW010000046         3908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_522920, whole genome shotgun sequence.
ACCESSION   DATITW010000046 DATITW010000000
VERSION     DATITW010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522920"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VLS86_00545"
     CDS             complement(<1..966)
                     /locus_tag="VLS86_00545"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HSO49005.1"
                     /translation="MAVLTVAGTTELVVGEGLPDQILPERAGRQRVAVVTQPGATHIA
                     LDVAQRLRDDDLHVEVIGLPDRDEAKTLVVAESVYEALARFGLGRHDTVVGVGGGSVS
                     DLAGFVAGTWLRGVEVVHIPTTLLAAVDAAIGGKTGVNLAGKNLVGLFWHPTRVIVDV
                     STLGRLPSSLRREGLAEALKAGLVGDPGLFELLAREGEQAPLEEVVTRAASVKAKVVG
                     EDERELGVRAVLNFGHTIGHAIEFASPLSHGQCVAVGMVAAGHISERRLRFPGLGPMV
                     EAIAGLGLPVEVDGLDRARVEDLLRHDKKRDATGVRMVLLRAIGEP"
     gene            complement(1001..2158)
                     /gene="aroC"
                     /locus_tag="VLS86_00550"
     CDS             complement(1001..2158)
                     /gene="aroC"
                     /locus_tag="VLS86_00550"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HSO49006.1"
                     /translation="MLRFMTAGESHGPGLVTIVEGLPRGLVVSADRISDELARRRAGY
                     GRGKRMAIERDQVEILGGIRHGETLGSPVAVLIRNTEWPRWSEEMSPEPAAAKKQVTR
                     PRPGHADLAGMLKYGTDDARDILERASARETAARTVAGVLAKILLETVGVTVVSHVIA
                     IGPVASEGPPPGPGDLDLVDASPVRCFDQAAETAMIEAIDDAKSQKDTLGGVFEVLGF
                     GIPPGIGSHVHYDRKLDALLAGAVMSIPAIKGVEIGDGFEVAGLPGSKSHDEIVLHDG
                     SLTRPTNRAGGSEGGISNGETIRVRAAMKPISTLMRPLRTVDMTTGEPAEAVRERSDV
                     CAVPAAAVVGEQMVAVVLAAEFQRKFGGDNVEELVTAVDRYKSGLEGRPGR"
     gene            complement(2170..2955)
                     /locus_tag="VLS86_00555"
     CDS             complement(2170..2955)
                     /locus_tag="VLS86_00555"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HSO49007.1"
                     /translation="MINVLAGAFGFLGAFIAHDLAAQSLADLPLRPLTGTCPRCGNPR
                     GWAHGVCGQCGRRIGRELLFALLAAGFAVAFANTLGQVWALLAYLGFLILTLALGITD
                     IDALRIVDRLNLRGTLIVVGALAVGSFADGTEAGFVRGLLGGAAYFVGTNLVFLLVRG
                     RGFGYGDVKLSAQLGVFTAFLSWGTLGWSVFLTALIGGVLSIGFLLAGVVTRARAKST
                     GGSGSAPSVRDAMKVELPYGPSMILGAWISITLAGLGAFPIPT"
     gene            complement(2999..>3908)
                     /locus_tag="VLS86_00560"
     CDS             complement(2999..>3908)
                     /locus_tag="VLS86_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49008.1"
                     /translation="LTNTGATFEVSEGATSPYGSTRPFELEAILDDCRRRADEWATVH
                     EVIADMDSTIRMNRDPGDREEIKINREAWRLLCELGSGASVSLMAERLGTTDFWIAKV
                     AAEMADQKLLVLTEAISETEAPISEVTADVEEVRDANESWWVEPQAEEPVADPSVASV
                     DPSVAPVDEEQEAAPSEPVKDSRFGHFVRATLQEPVAPVEESVADAVTESFAPAAPES
                     FAPAAPESFAPTAPEAEAAGDVYDETPVFEPVDETEDVEEDTEAFLEKVFSQLEVNGQ
                     ADVEEEGHGLLRRRRMGSVLKEIDEN"
BASE COUNT          655 a         1373 c         1271 g          609 t
ORIGIN      
        1 cggctcgccg atcgcccgca gcaggaccat cctcacgccc gtcgcatcgc gcttcttgtc
       61 atgacgaagc aggtcctcga cgcgggctcg atcgagcccg tccacctcga cggggagacc
      121 gagacctgcg atggcctcga ccattgggcc caacccgggg aaacgcaacc ggcgctcgga
      181 gatgtgcccg gcggcaacca tccccaccgc cacacactgc ccgtgagaca acggggaggc
      241 gaactcgatg gcatggccga tggtgtgacc gaaattgagg accgcccgga ccccgagctc
      301 acgctcgtcc tcgcccacga ctttcgcctt caccgaggcg gcacgggtca ccacctcctc
      361 gagcggggcc tgctctccct cccgggccag cagctcgaac aggccagggt cacctaccag
      421 cccggccttg agcgcttcgg ccagaccttc cctgcgcagc gaggagggaa gccgccccag
      481 ggtgctcacg tccacgatca cccgggtcgg gtgccagaac aggccgacca ggttcttgcc
      541 ggccagattt acgccggtct tgcccccgat ggcggcgtcg acggcggcga gcaacgtggt
      601 cgggatatga accacctcga cccccctcag ccaggtgccg gccacgaacc cggcgaggtc
      661 ggagactgat cccccgccga caccgaccac ggtgtcgtgg cgccccaacc cgaatcgggc
      721 cagcgcctcg tacaccgact cggccaccac cagtgtcttg gcttcgtccc ggtcggggag
      781 gccgatcacc tcgacgtgga ggtcgtcgtc gcgcagacgc tgggcgacgt ccagcgcgat
      841 gtgagtcgcc cccggctggg tcacgacggc gacacgctgg cgtccggcgc gctcggggag
      901 gatctggtcg ggaagaccct cgcccacgac cagctccgtc gtgccggcaa cggtcaggac
      961 ggccacaggc cctcgattct tatcccgatc aaccagaggg tcatctgccg ggccggccct
     1021 cgagacccga cttgtagcgg tcgactgcgg tgacgagctc ctcgacgttg tccccgccga
     1081 acttccgttg gaactcggcg gccaggacca ccgccaccat ctgctccccc accaccgcgg
     1141 cggcggggac ggcgcatacg tccgagcgct ctctcaccgc ctccgccggc tcccctgtgg
     1201 tcatgtccac ggtgcgcagt ggtctcatca atgtcgagat gggcttcatc gccgcccgga
     1261 cccggatggt ctcaccattg ctgatcccac cttcgctccc gccggcccgg ttggtggggc
     1321 gggtgaggct cccgtcgtgg agaactatct cgtcgtgcga tttggagcca ggaagaccgg
     1381 cgacctcgaa gccgtctccg atctcgaccc ctttgatcgc cgggatcgac atcacggcac
     1441 cggcgagcag ggcgtcgagc ttccggtcgt agtggacgtg cgagccgatc ccggggggaa
     1501 tgccaaagcc gaggacctcg aacacgcctc ccagggtgtc cttttgcgac ttggcgtcgt
     1561 cgatcgcctc gatcatcgcg gtctccgctg cctggtcgaa acagcgcacc gggctggcat
     1621 ccaccaggtc caggtcgcca ggcccgggtg gggggccctc actggccacc gggccgatgg
     1681 cgatgacgtg actgaccacc gtgactccca cggtctcgag gaggatcttg gccaggaccc
     1741 cggccacggt gcgggcggcg gtctcccggg cggaggctct ctccaggatg tcccgagcgt
     1801 cgtcggtccc gtacttgagc atgcccgcca ggtcggcatg accgggccga gggcgggtca
     1861 cttgcttctt ggcggccgcc ggctccgggc tcatctcctc ggaccatcgc ggccattccg
     1921 tgttgcggat caagactgca accggcgagc cgagggtctc accatggcgg atcccgccga
     1981 gaatctcgac ctggtcccgc tcgatggcca tgcgcttgcc ccggccgtaa cctgccctcc
     2041 tcctggccag ctcatcggag atccggtccg cggagaccac cagtccgcgc ggcagccctt
     2101 ccacgatcgt caccaggccc ggcccgtgtg actcgccggc tgtcatgaag cgcaacatgg
     2161 gggcggatgc tacgtcggga tcgggaaagc tccgagtccg gcgagggtga tcgatatcca
     2221 cgccccgaga atcatcgacg ggccgtaggg gagctcgacc ttcatcgcgt cgcgaaccga
     2281 cggcgccgac ccactccctc cggtgctctt cgctcgcgcc cgggtgacga cgccggcgag
     2341 gaggaagccg atcgagagga ctccgccgat cagggcggtg agaaaaacgc tccaacccaa
     2401 tgtcccccag gagagaaagg cagtgaaaac cccgagttgt gcggagagct tcacgtcgcc
     2461 gtaaccgaac ccccggcccc ggacgaggag gaacacgaga ttggtgccga cgaagtatgc
     2521 ggctccgccc agtaggccgc gaacgaaccc ggcctctgtg ccatcggcga atgagccgac
     2581 tgccagggcg cccacgacga taagcgtgcc acgcagattg agccggtcga cgatgcgcag
     2641 ggcgtcgatg tcggtgatgc cgagggcaag ggtcaagatg agaaagccga gataagcgag
     2701 cagggcccag acctggccca gggtgttggc gaacgcgacg gcgaacccgg ccgccaacag
     2761 cgcgaacagg agctcacgac cgatccggcg accacattgc ccgcagacgc cgtgtgccca
     2821 gcccctcggg ttgccgcatc gagggcaagt cccggtcaag gggcggagcg gtagatcggc
     2881 cagcgactgt gctgccaggt cgtgggcgat gaaggcccca agaaagccaa atgctccggc
     2941 gaggacgttg atcaccgccg gagcaaccta gccgcggtcg tggggacgac cggtcaaatc
     3001 agttttcgtc gatctccttg agcaccgagc ccatgcggcg gcggcggagg agaccatgcc
     3061 cctcctcctc gacgtcggcc tggccgttca cttcgagctg ggagaacacc ttctccagga
     3121 aggcttcggt gtcctcttcc acgtcctcgg tctcgtccac cggctcgaag acgggggtct
     3181 cgtcgtagac gtccccggcg gcctcagcct cgggagcggt gggagcgaag ctctcgggag
     3241 cggccggggc aaagctctcg ggagcggccg gagcaaagct ctcggtaact gcgtcggcga
     3301 cgctctcttc caccggagcg accggttcct ggagcgtggc tctgacgaaa tggccaaacc
     3361 ggctgtcctt gaccggctcg ctcggtgcag cctcctgctc ctcgtcgacc ggggcaacgc
     3421 ttggatcgac cgaggcaacg cttggatctg cgaccggctc ctctgcctgg ggctcgaccc
     3481 accacgactc gttcgcgtcc cgtacttctt cgacgtcggc ggtgacctct gagatcgggg
     3541 cctcggtctc cgagatcgcc tcggtcagga ccaggagctt ctggtcggcc atctcggctg
     3601 ccacctttgc gatccagaag tcggtcgtgc ccaaacgctc cgccatcagc gacaccgaag
     3661 cgccggagcc aagctcgcag agcagacgcc acgcctcgcg gttgatcttg atctcctcac
     3721 ggtcaccggg atcacgattc atccggatcg tggagtccat gtcggcgatg acttcgtgga
     3781 cggtggccca ctcgtcggcg cgacgacggc agtcgtccag aatcgcctcg agctcgaaag
     3841 ggcgggtgct gccgtagggt gacgtagcgc cctcggagac ctcgaaagtc gcacccgtgt
     3901 tggtcagg
//
LOCUS       DATITW010000047         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_442985, whole genome shotgun sequence.
ACCESSION   DATITW010000047 DATITW010000000
VERSION     DATITW010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442985"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /locus_tag="VLS86_00565"
     CDS             <1..1733
                     /locus_tag="VLS86_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSO49009.1"
                     /translation="RIALIGLFLVAAWGGMAYRLVQVQVVQAAELANKGLSQRLVSRE
                     LPPQRGRIYDRNGDLLAMTVESQSLYAVPGQVEDPLWVAQQLGGVLGVDSDVLFDRLT
                     SERDFVYLKRQVEAELAEEVLGLEVKGVYSLPEPNRIYPAGQVASHVTGFVDIDGNGQ
                     EGLEYVYDEQLRGIQGHALFERDLDGRPIPQGISEIVPAVPGVDLNTTIDLPLQFQAQ
                     EACLDAVERTDAKSCWVVVLAVETGEVLAMTGAPAFDPMTRENVDPRCADDPDPAGCL
                     AFSNFIVRGLYEPGSTEKLITVAAAIEEGEVDDTTVIPDVADVLELREDACRSADDDT
                     YGCYRDFDEHETSDLTVAEIFTQSSNVGSILVAEKLGQERLISYIEAFGLGSKTGVDY
                     SVEAPGLLNFAAGCETCWASAAIGYSVAVTPLQMASAYAAIGNDGVWTTPHIVSTMTD
                     VDGDTDVAGYETRRVVSQETAVLMRTLLANVVDEGTGQGATIEGYRVGGKTGTANILG
                     EDGRYTETTRASFVGMAPIDDPKVVVAVLVDSPSWDYRTGGASAAPVFAEVMEEALHR
                     LGVTPDAGQG"
     gene            1738..>2051
                     /locus_tag="VLS86_00570"
     CDS             1738..>2051
                     /locus_tag="VLS86_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HSO49010.1"
                     /translation="MAELAVAVGGTTHGDPDVTVTDVTHDSRHAAEGTMYVAVRGAHV
                     DGHDFIDSAVAAGSTAVCVDHFTGSEVSELEVDDTRLVMGPLAAAVHDDPSATVAVVG
                     VTG"
BASE COUNT          361 a          643 c          693 g          354 t
ORIGIN      
        1 tgcgaatcgc actgatcggc ctctttctgg tcgcggcatg gggcggcatg gcatatcggc
       61 tggttcaggt tcaggtggtg caagcggccg aactggcgaa caaagggcta tcccaacgtc
      121 tcgtgtcgag agagctgccg ccacaacggg gcaggatcta cgaccggaac ggcgacttgc
      181 tggcgatgac cgtcgagtcc cagagcctct acgcggtgcc cggccaggtg gaagatccgc
      241 tgtgggtcgc ccagcagttg ggaggggtcc tcggagtcga ttccgatgtc ctcttcgacc
      301 gcctcacctc ggagcgtgac ttcgtctacc tcaagcgaca ggtcgaggcc gaactggccg
      361 aagaggtcct cgggctcgaa gtcaaggggg tctactccct tcccgagccc aaccgcatct
      421 acccggccgg ccaggtggcg agtcatgtga ccggcttcgt ggatatcgac gggaatggcc
      481 aggaagggct ggagtatgtc tacgacgagc agctgagggg gattcaggga cacgccctct
      541 tcgagcgcga cctggacggg aggccgatcc cccagggaat cagcgagatt gtgcccgcgg
      601 tgcccggcgt cgatctcaac accacgatcg acctgcccct gcaattccag gcccaggagg
      661 cctgtctcga cgctgtggag cgcacagacg ccaagtcgtg ctgggtagtg gtgctcgcag
      721 tcgagaccgg cgaagtcctg gcaatgaccg gggcgcctgc cttcgacccg atgacccggg
      781 agaacgtgga tcctcgatgc gccgacgatc ccgacccggc cggctgcctc gccttctcca
      841 acttcatcgt gcgcggtctc tatgagccgg gctcgaccga gaagctgatc acagttgccg
      901 ccgccatcga agagggagag gtggatgaca ccacggtgat cccggatgtg gccgacgtcc
      961 tggaactgag agaagacgcc tgtcgttcag ccgatgacga cacctacggc tgctatcggg
     1021 acttcgacga acatgagaca tcggacctga ccgtggccga gatcttcacc cagtcgtcga
     1081 acgtgggctc gatcctggtc gccgagaaac tgggtcagga acgtctgatc tcttacatcg
     1141 aggcgttcgg ccttggttcc aagaccggcg tcgactactc ggtcgaggcg ccggggctgc
     1201 tcaacttcgc cgccggatgt gagacctgct gggcctccgc tgccatcggc tactcggtgg
     1261 ccgtgacccc gctgcagatg gcatccgcct atgcggcaat cggcaacgac ggggtctgga
     1321 ccacccctca catcgtttcg accatgaccg atgtggacgg cgacaccgat gtcgccgggt
     1381 acgagactcg ccgggtcgtc tcgcaggaga cggcggtgct gatgcgaacc ttgctcgcca
     1441 atgtcgtcga tgagggcacc gggcagggag ccacgatcga gggctaccgc gtgggcggca
     1501 agaccgggac cgccaacatc ctcggcgagg atgggcggta cacggagacc acccgggcct
     1561 cgtttgtggg aatggcgccc atcgacgacc cgaaggttgt ggtggcggtc ctcgtcgatt
     1621 cgccttcctg ggactaccgc accggtgggg catcggccgc cccggtgttc gccgaggtca
     1681 tggaagaagc cctacatcgt ctcggagtga cccccgatgc cgggcagggg taagaagttg
     1741 gctgaactgg ccgtcgcggt cggggggacg acgcatggcg accccgatgt gaccgtcacc
     1801 gatgtcaccc acgattcgcg acacgccgcc gagggaacca tgtatgtggc tgtccgggga
     1861 gcgcacgtcg acggccacga cttcatcgac tcggcggtag ctgccggctc gaccgcggtc
     1921 tgcgtagatc atttcacggg gagcgaggtc tccgagctcg aggtggacga cacccggttg
     1981 gtcatgggcc cgctcgccgc agcggtgcat gatgatccat cggccaccgt ggccgttgtc
     2041 ggcgtcaccg g
//
LOCUS       DATITW010000048         1775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_122478, whole genome shotgun sequence.
ACCESSION   DATITW010000048 DATITW010000000
VERSION     DATITW010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122478"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="VLS86_00575"
     CDS             <1..1385
                     /locus_tag="VLS86_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HSO49011.1"
                     /translation="EEIRVVLALIETVRDGPVFIESVRASLARGKPVVSCRIGTGRHG
                     KTLMTTHTGAMAVPEAVLEGVLEYLGVVIAETPGEAYDIAEVMARVAVPAGPRVAIVT
                     HSGGIAIHLADLAERHRIELADAGPELQTRLAPLLDLGAANNPLDMGGIIGGPGRFGE
                     VVETFAESGEYDMVLAVSTAHPPAHTEDRVTALIARSAVVDVPIVHLWMAGDQAGEGL
                     RVLRAAGAALTEEPRAAVRAMAALAALSRRIDDAAVEPITGAIESWGLPMVEGGFAAD
                     PSQAVAVAERIGYPVVVKVSAPGMAHKTEVGGVRLDLKDAKAVMAGFDDVTASGRAAG
                     LPVAGARVERFRPGLEMIVGGLVDPVFGPVVSVGIGGVMTELLDDVVFAPAPVTKEAA
                     LKMIDRLGGRRLLDGFRGSDPPDVLGLARVISLLSRGLVGSGLTEVELNPLIWDGEWV
                     AVDWLVGAGL"
     gene            complement(1392..>1775)
                     /locus_tag="VLS86_00580"
     CDS             complement(1392..>1775)
                     /locus_tag="VLS86_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HSO49012.1"
                     /translation="EDRPERSGEICVAEVFGETILDGWSEVGIGVKRLGDTALAQEFS
                     AHRIEIDVSEFHTYGVEWRPGHLEYAIDGQVVSRDVQAPDYPMQLMIGVFDFPAKAHA
                     TDDDVPVPELIVSHVRGRSWATSTT"
BASE COUNT          264 a          533 c          665 g          313 t
ORIGIN      
        1 tggaggagat tcgagtcgtg ctggcattga tcgagacggt ccgtgacggg ccggtgttca
       61 tcgagtcggt ccgcgcctct ctggcccggg gaaagccggt ggtgtcctgt cgcatcggca
      121 ccgggcgcca cggaaagacc ctgatgacca cccacaccgg ggcgatggcg gttcccgaag
      181 cggtgctgga aggtgtcctc gaatatctgg gcgtggtgat cgccgagaca ccgggagagg
      241 cctacgacat cgcggaggtg atggcccgag tggccgtgcc cgccgggcct cgggtcgcca
      301 tcgtcactca ctccggaggc atcgccatcc atctcgccga tctggccgag agacatcgga
      361 tcgagttggc cgacgcgggc cccgagctcc agacccggct cgccccgctg ctcgacctcg
      421 gtgcggccaa caacccgttg gacatgggcg ggatcatcgg tgggcccggt cgattcggag
      481 aggtggtgga gacattcgcc gagtcgggcg agtacgacat ggtgctggcg gtgtctacgg
      541 cgcaccctcc ggctcatacc gaggatcgag tgaccgcgct gatcgcccgc agcgcagtcg
      601 tcgacgtgcc gattgtccat ctgtggatgg caggcgacca ggccggcgag ggcctccgcg
      661 tgctgcgtgc cgccggcgcg gccctgaccg aggagccgag ggcggcggtg cgggccatgg
      721 cggcgttggc cgcgctgagt cggcggatcg acgacgcagc cgtggagccg atcaccggcg
      781 ccatcgaatc ctggggtctc cccatggtcg aaggagggtt tgccgccgac ccttcccagg
      841 cggtggctgt ggcggaacgt atcggctatc cggtggtggt aaaggtctcg gctcccggta
      901 tggcccacaa gaccgaggtc gggggagtga ggctggattt gaaggacgcg aaggcggtga
      961 tggctggatt cgacgatgtg acggcttcgg gaagggctgc cggtctgccg gtcgccgggg
     1021 cacgggtgga gagattccgt cccggtctgg agatgatcgt cggtggcctg gtggacccgg
     1081 tcttcggccc ggtggtttcg gttgggatcg gcggtgtcat gaccgagctt ctcgacgacg
     1141 tggtattcgc gccggccccg gtcaccaagg aagctgcgct gaagatgatc gaccgactgg
     1201 gcgggcggcg tctgctcgac gggttccggg ggagtgatcc ccccgatgtc ctgggcctcg
     1261 cccgtgtcat cagcttgttg agcaggggtc tggtcggctc gggtctgacc gaggtggagc
     1321 tcaatcccct gatctgggac ggcgagtggg tggcggtgga ctggctggtc ggtgcaggtc
     1381 tctgactgga gtcaggtagt ggaggtggcc cagctgcggc cgcggacatg ggagacgatt
     1441 agttcaggga ccggcacgtc atcgtcggtc gcgtgggcct tggccgggaa gtcgaacacg
     1501 ccgatcatga gctgcattgg gtagtcgggc gcctggacgt ccctggaaac cacctggccg
     1561 tcgatggcgt actcgaggtg ccccggtcgc cactcgaccc cgtaggtgtg aaactcggag
     1621 acatcgatct cgatccggtg agctgagaac tcctgggcca gtgccgtgtc gccgagcctc
     1681 ttcacgccga tgccgacctc ggaccagcca tcgaggatcg tctcgccgaa cacctcggcg
     1741 acgcagatct cgccggagcg ctcggggcga tcctc
//
LOCUS       DATITW010000049         1507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_763543, whole genome shotgun sequence.
ACCESSION   DATITW010000049 DATITW010000000
VERSION     DATITW010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763543"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VLS86_00585"
     CDS             <1..438
                     /locus_tag="VLS86_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HSO49013.1"
                     /translation="HDFIGRSALEDMSQEPPRTKVTLALDQGDVLGIIASQLEPADRG
                     KFIEFPSAVYAMHPYDAVVIDGETVGISTWIGYSSNERKMLALAVIANEFAQPGTEVT
                     LLWGEADGGSKKVNVERHQQTVIRATVSPVPYSEYARRSYRAD"
     gene            complement(386..>1507)
                     /locus_tag="VLS86_00590"
     CDS             complement(386..>1507)
                     /locus_tag="VLS86_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSO49014.1"
                     /translation="GGGGGDHPHPSLRPARGAGMNDRFIEATGSSGICGVEHRASRSL
                     IALVWPILALGAVLALVPLWLGGSRVMMGVSVVGLVFACYAIGFNLIFGSTGQLFLCV
                     GALAGIGGYGSAILADTVGLPLPLSMLLATAAASLLGGLLSWTAVRRSLGVIFTGIIT
                     LIFSLVFGNLLLGAGGVTGGDGGFRVQTGAGTFLRQPIPPYYLMLTLVLSYLLIHGLL
                     RRSRAGWAFRALRDDEVAAELAGVDVARYRVYAAVVGSAMLGLTGSVYAITEGTISPS
                     TYGFAQVDVETMVMLAFGGIGTLFGPILGAAIVTVINELTLDLGQLRLLVYGALIIAL
                     FLWVPRGVIPTLRDLLRRRARTSSRPDTTAAHTPSTGPG"
BASE COUNT          310 a          501 c          494 g          202 t
ORIGIN      
        1 cacgatttta tcggtcgttc cgccctcgag gacatgtccc aggaaccgcc tcggaccaag
       61 gtgactctcg ccttggacca gggagacgtt ctcgggatca tcgcatcgca gctggagccc
      121 gcggaccgtg gcaagttcat cgagttcccc tcggccgtct acgccatgca tccctacgat
      181 gcggtcgtga tcgacgggga gacggtggga atctccacct ggatcggcta cagctcgaac
      241 gagcgcaaga tgctggccct tgcggtgatc gccaacgagt tcgcccagcc cggcaccgag
      301 gtgaccctgc tctgggggga ggcggacggc ggctccaaga aggtcaacgt ggagcgccac
      361 caacagaccg tgatccgggc gaccgtcagc ccggtcccgt actcggagta tgcgcggcgg
      421 tcgtatcggg ccgactagac gtgcgcgccc gccgccggag caggtcgcgg agcgtgggga
      481 tcacaccccg gggcacccaa aggaacaagg cgatgatgag cgccccgtat acgagcaagc
      541 ggagctggcc cagatcgagg gtcaattcgt tgatgacggt gacgatggcc gctcccagga
      601 tggggccgaa gagggtgcca atgccaccga aggcgagcat caccatcgtc tccacgtcga
      661 cctgggcgaa cccataagta gaggggctga tcgtgccctc ggtgatcgcg tagacggacc
      721 cggtcaaccc cagcatggcg gagcccacca cggcggcata cacccgatat cgggcaacgt
      781 cgactccggc caactcggct gcaacctcgt cgtcacggag ggctcggaag gcccagccgg
      841 cacgggaccg gcgcaacagg ccatggatca atagatagct gagcaccagg gtcagcatca
      901 agtaataggg cgggatcggc tgtcggagga aggtcccggc tcccgtctgg acacgaaagc
      961 ccccgtcgcc cccggtaaca ccgcccgccc cgagcaggag gttcccgaac accagcgaga
     1021 agatgagtgt gatgatcccg gtgaagatca ccccgaggga gcggcgcacc gcggtccagc
     1081 tgagcagacc ccccagcagg ctcgctgccg cggtggccag gagcatgctc agaggcagcg
     1141 gcaatcccac agtgtcggcg aggatggccg atccgtaccc cccgatcccc gccagggccc
     1201 cgacgcatag gaacagttgc ccggtacttc cgaagatcag gttgaacccg atggcgtagc
     1261 aagcgaagac cagaccgacc accgacacgc ccatcatcac ccgagagccg ccgagccaga
     1321 gagggacgag ggcgagtacc gcacccaggg ccaggatggg ccagaccagg gcaatcagtg
     1381 accgtgatgc tcggtgctcc accccgcaga tgccggagct gccggtcgcc tcgatgaatc
     1441 tgtcgttcat tcccgcaccc cgagcaggcc ggagggacgg atgaggatgg tcaccgccgc
     1501 cgccacc
//
LOCUS       DATITW010000050         1637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_402872, whole genome shotgun sequence.
ACCESSION   DATITW010000050 DATITW010000000
VERSION     DATITW010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402872"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VLS86_00595"
     CDS             <1..331
                     /locus_tag="VLS86_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49015.1"
                     /translation="IRTTSLALGVVATALALSAGALFQASSRGADLAPIAGAETLVRV
                     LQENQNVLWTSLRGNVTSYVVQGWPSSSGDTELGGAMMMERPASGEAGVIDFGEKPMT
                     LRFEIAR"
     gene            complement(328..1581)
                     /locus_tag="VLS86_00600"
     CDS             complement(328..1581)
                     /locus_tag="VLS86_00600"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSO49016.1"
                     /translation="MHYDLTTLPNGIRVITEPMPSLRSIALGCWVDTGTRDELPNEQG
                     ASHFLEHLLFKGSETLSAREVSEIFDSIGAESNAFTSKENTCYWTRLLDQDLGTGFDV
                     LAEMIQRPAFRQNEIDAERQVVIEEINMNEDDPDDVAFENFTRAAFSGHPLEAPVLGT
                     RESIRGMSRDDILGYWKRRYGAGSMVVAGAGSLEHEAVVEMVSARFGDWSGEAVDHEF
                     GPSTPVSTISITRRDTEQAHIILGGQGINRSDERRWAFEVMNHVVGSGMSSRLFREVR
                     EERGLAYAIYGFKLAYADAGAWGVYVGTTPSQADTALNVIRDELSKVVADGITPEELE
                     RAKGSMRGGLALSLEDPNSRMVRLGRDELSGMPHLSVDERLAKLEAVTLSDVQSIATD
                     LFTGERILGAVGPFDESDLEPYLVA"
BASE COUNT          303 a          512 c          524 g          298 t
ORIGIN      
        1 aattcgcacc acctcgctcg ccctgggcgt cgtggccacg gcgctggccc tgagcgccgg
       61 cgccctgttc caggcctcaa gccggggagc cgacctcgcc cccattgcgg gcgccgagac
      121 actggtccgg gtgctccagg agaatcagaa cgtcctatgg acctcgctca gggggaacgt
      181 gaccagctac gtggtgcagg gatggccttc gagctccggt gacaccgagc ttggcggagc
      241 catgatgatg gagcgccctg cgagcggtga ggccggtgtc atcgattttg gcgagaaacc
      301 aatgacgctt cgcttcgaaa tcgcccgcta ggcgaccaaa tagggctcga ggtcggactc
      361 gtcgaaggga ccgacggcac ccagaatccg ctccccggtg aacagatcgg tcgcaattga
      421 ctggacgtcg gagagggtga ccgcttccag cttggccagt ctctcatcga cggagagatg
      481 gggcattcct gagagctcgt cacgccccag tcgcaccatc ctgctgttcg gatcctccag
      541 tgacagggca aggccacccc gcatcgaccc cttggcgcgc tccaactcct cgggtgtgat
      601 cccatctgcc accaccttcg acagctcgtc gcgaatcaca ttgagggcgg tgtcagcctg
      661 actcggggtg gtcccgacgt agacgcccca cgccccggcg tcggcgtaag cgagtttgaa
      721 cccgtagatg gcataggcga gacctcgctc ttcgcgcacc tcgcggaaga gacgggacga
      781 catgcccgag cccaccacgt gattcatgac ctcaaacgcc caacgacgct catcggatcg
      841 gttgatgccc tgacccccaa ggatgatgtg ggcctgctcg gtgtcgcgac gagtgatcga
      901 tatggtcgag accggtgtgc tcgggccgaa ctcgtggtcg acggcctcgc ccgaccaatc
      961 cccgaaccgt gcggacacca tctcgacgac ggcctcgtgc tcgagggatc ccgcccctgc
     1021 cacgaccatg gaaccggctc cgtaacgccg cttccagtat ccaaggatgt cgtcgcggga
     1081 catcccacgg atcgactcgc gggtccccag caccggagcc tccaaggggt gaccggagaa
     1141 ggcggcccgg gtgaagttct caaaggcaac atcgtcggga tcgtcttcgt tcatgttgat
     1201 ctcttcgatg acgacctgac gctcggcatc gatctcgttc tgacggaatg ccggtcgttg
     1261 gatcatctcg gcgaggacgt cgaagccagt tcccaggtcc tgatcgagca gacgggtcca
     1321 gtaacaggtg ttctccttgg aggtgaaggc attcgactcg gcgcctatcg agtcgaagat
     1381 ctccgagacc tcacgagcag acaatgtctc cgagcccttg aacagcagat gctcgaggaa
     1441 gtgagaggcg ccctgttcgt tgggaagctc gtctcgggtt ccggtgtcga cccagcaccc
     1501 gagggcgatc gatcgcagcg agggcatggg ctcggttatg acgcggatgc cgttgggcag
     1561 ggtggtgagg tcgtagtgca cgccgggagt gtatgggagg ctgcgcggta cttagtactt
     1621 ggtactgggt acttgga
//
LOCUS       DATITW010000051         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_603207, whole genome shotgun sequence.
ACCESSION   DATITW010000051 DATITW010000000
VERSION     DATITW010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603207"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VLS86_00605"
     CDS             <1..693
                     /locus_tag="VLS86_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49017.1"
                     /translation="HRRDTREPAPRRELRTVTSVSSGQLPKWVREEIQRSTPKDRRDP
                     ALNHLAKALNQFADERYGAALPELRKAKTLAPRSSTVREMLGLSAYHTGNWEEALREL
                     RTFRRLTGDLIHMPMEMDSLRALGRKGDVMKTWDLLQELDTSATVSHEAKVVFASFLL
                     DEGKPREAWDVVRPGRLVASPSPGELRRWYVAAKAAIAAGDLDAARRLVAALDEQDPD
                     FEGVDELRTALL"
     gene            761..1090
                     /locus_tag="VLS86_00610"
     CDS             761..1090
                     /locus_tag="VLS86_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSO49018.1"
                     /translation="MDLNLVVLCGRLATDPELRAFDSGTQLARLLVTVRSDEPRRRVD
                     VVPVTLWDPPAEFLEDPPEKGCRVWVCGSVQRRFWESPDGRRSRLEVVAEQVHVKDVE
                     DLEPVSV"
     gene            1179..1355
                     /locus_tag="VLS86_00615"
     CDS             1179..1355
                     /locus_tag="VLS86_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49019.1"
                     /translation="MPWYWTDDIARALIDAGRLTADAAGPMLSVPVAIRRDESSIEAA
                     ARALVDDGEIPLAA"
     gene            1359..2090
                     /locus_tag="VLS86_00620"
     CDS             1359..2090
                     /locus_tag="VLS86_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HSO49020.1"
                     /translation="MRWLLSPRWVVAHIVVLALAVVFVNLGFWQLRRLDERRLTNQVG
                     ESRYAATPVDLAELLSAAGDDVESLEYRRATATGVFQPDQEILVRSQVYQGAAGFHVI
                     TPLLGESGSAVLVNRGWVPLGLDTVPVVEAPPPEGEVTVTGWVRPTQERMSLGPTDPE
                     DGRLVALNRVDIDRIEEQVPFELAPVYLSQLGDQEPDRPLLVSAPTFDDEGPHLAYAV
                     QWFGFMVIGVVGYLMLIRRVSRRSG"
     gene            complement(2082..2573)
                     /locus_tag="VLS86_00625"
     CDS             complement(2082..2573)
                     /locus_tag="VLS86_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HSO49021.1"
                     /translation="MTADPSVLESMRALGIEFEEMECDPALADTAQFCEAYGVPLDRS
                     ANTILVASRKPEGMMVACLVLATTRLDVNDVVRRKMGARKASFANAEQTMEVTGMEIG
                     GVTIFGLPEDMAVWVDTRVVDCEWVIIGAGSRSAKIRLDPRQLIGLDGFEVVDGLARE
                     ASA"
BASE COUNT          434 a          897 c          924 g          492 t
ORIGIN      
        1 caccggcgcg acacccggga gccggctccc cggcgcgagc tccgaaccgt gacctcggtg
       61 agctcgggtc agctcccgaa atgggtgcgc gaggagatcc agcgctccac tcccaaggac
      121 cggcgcgacc ccgccctcaa ccacttggcc aaggccttga accagttcgc cgacgagcga
      181 tacggcgccg cacttcccga gctgcgcaag gcgaagaccc tcgccccgcg ttcgtccacg
      241 gtgcgggaga tgctcggcct gtccgcgtat cacaccggca actgggagga ggcgctccgg
      301 gagctgagga cctttcgccg tctcaccggc gatctgatcc acatgccgat ggagatggat
      361 tcgctgcgcg ccctggggcg caagggcgac gtgatgaaga cctgggacct gctccaggag
      421 ttggacactt cggccacggt ctcacatgag gccaaggtcg tgtttgcctc cttcctgctc
      481 gacgaaggca agccacgcga ggcgtgggat gtcgtgcgac cgggtcgcct ggtcgcctct
      541 ccctccccag gagagcttcg ccgctggtat gtggcggcga aggccgcgat cgcggccggg
      601 gacctggacg ctgctcgacg gctggtggcg gctctcgacg agcaggaccc cgatttcgag
      661 ggcgtcgacg agctgcgcac cgcccttctc tgatctgtca ctgacagccg gcatagtcgt
      721 ctcatctgcg cttccccgac gatgagtagg ggagagtgtc atggatctca acctggttgt
      781 cctctgtgga cggcttgcca ccgatcccga gctgagggcc ttcgattcgg gaactcagtt
      841 ggcccgattg ctggtgacgg tcagaagcga cgaaccaaga cggcgagtcg acgtggtacc
      901 ggtcacattg tgggacccgc ccgcggaatt cctcgaggac ccaccggaga aagggtgccg
      961 ggtctgggtg tgcggctcgg tgcagagacg gttctgggag tcgcccgacg ggcgccggtc
     1021 gcgtctcgaa gtcgttgccg agcaggtgca tgtcaaggat gtcgaagacc tggagccggt
     1081 gtcggtttag cccgtgttca ttggcgattt cggggattga cagcgggggt acagtggcca
     1141 ggtccctgtc ccctgacatc ttctctggga ggttggccgt gccctggtat tggactgatg
     1201 acatcgcccg agccctgatc gacgcaggtc ggctcactgc cgacgccgcg ggcccgatgt
     1261 tgtcggtgcc ggttgcgatc cgtcgtgacg agtcgagcat cgaggcagca gcccgggctc
     1321 tcgtcgacga cggtgagatc ccgctagccg cctagcgggt gaggtggctg ctcagcccac
     1381 gatgggtcgt ggcccacatc gtggtgctcg cgctggcagt cgtcttcgtc aacctcggct
     1441 tctggcagct ccgtcgcctg gatgagcgcc ggctgaccaa tcaggtgggg gagtcgcgat
     1501 acgcggccac acccgtcgac ctcgcggagc tgctctctgc tgccggcgac gacgtcgaga
     1561 gcctcgagta tcgacgggct accgccacgg gcgtgttcca gcccgaccag gagatcctgg
     1621 tcagaagcca ggtctaccaa ggggctgcag gcttccacgt gatcaccccg ctgctggggg
     1681 agagcgggtc ggcggtgctg gtcaataggg gttgggtccc actcgggctg gacacggttc
     1741 cggtggtgga agcgcctccg cccgaaggcg aggtcaccgt cacgggttgg gtgcgaccca
     1801 cgcaggagcg gatgtccctg gggcctaccg accccgagga cgggcgtctg gtcgccctga
     1861 accgggtcga catcgatcgc atcgaggagc aggtgccgtt cgagctcgcc cctgtctacc
     1921 tgtcacagct gggggaccag gagccggatc gaccgcttct cgtctccgct cccaccttcg
     1981 acgacgaggg cccccacctt gcctacgcgg tccagtggtt cggattcatg gtgatcgggg
     2041 tggtcgggta cctgatgctg atcagacgcg tgtcgagacg ctcaggctga ggcttcgcgg
     2101 gccaggccgt cgaccacctc gaagccgtcg agcccgatca gctgacgggg atcgagccgt
     2161 atcttggcgc tcctcgagcc ggccccgatg atgacccatt cacagtcgac gacccgcgtg
     2221 tccacccaca ccgccatgtc ctcggggagg ccgaagatcg tgaccccgcc gatctccatc
     2281 cccgtgacct ccatggtctg ctcggcgttg gcgaaactcg ccttcctcgc tcccatcttc
     2341 cgtcgcacca cgtcgttgac atcgagacgc gtggtggcca ggaccagaca ggcaaccatc
     2401 atcccttccg gtttccggga tgcgacgagg atggtgttcg ccgatcgatc gagaggcacg
     2461 ccgtaggcct cacagaactg agcagtgtcg gccagggccg ggtcacactc catctcctcg
     2521 aactcgattc cgagtgctcg catcgactcg aggactgatg ggtcggccgt catgccgggg
     2581 agagtaggta attggcggac gaacccgggg ccgtgtgcga gagttccccc atgagcgagt
     2641 acgacatcgg ctcctacggc tccttctggg cgccctatta cgacgagatc ttccgctcgg
     2701 ccgacgcgga ggtcgccttc ctgctgccgc tggccgggaa cccgccg
//
LOCUS       DATITW010000052         5201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_162658, whole genome shotgun sequence.
ACCESSION   DATITW010000052 DATITW010000000
VERSION     DATITW010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162658"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VLS86_00630"
     CDS             <1..594
                     /locus_tag="VLS86_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HSO49022.1"
                     /translation="LDIGVDEWGDHFEETHVSWSNALHARRKGGGSYHLGPMARYSLN
                     SGQLTPVAAEAAHEAGLGPTCHNPFRSIVVRAVEIAFACEEALRLIDGYTQPPIPAIE
                     VEPRAVTGHGASEAPRGTLYHRYAIDPDGLITEAHIVPPTSQNQLSIEDDLRAYVVGR
                     AHLPDDQLQWQLEQAIRCYDPCISCATHFLDLTVDRG"
     gene            591..1016
                     /locus_tag="VLS86_00635"
     CDS             591..1016
                     /locus_tag="VLS86_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HSO49023.1"
                     /translation="MRRIVIGVGNPVRGDDGAGLEVAGRLRRVETYQRSTGSYELIDL
                     WDGADEVIVVDAARSGAPAGTIHRFDAHERPLPEGVLATSTHSVGVMETIEMARSLGR
                     LPERVVVYGIEVSTLSPGSGLSDVVEQAVNELVTVIDNA"
     gene            1039..1269
                     /locus_tag="VLS86_00640"
     CDS             1039..1269
                     /locus_tag="VLS86_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49024.1"
                     /translation="MSRVEEEADVGNGQVTRLRFRVGALAGIGSESLIEGARHYAIEN
                     WGYAPEVLVEEAADPTDPNALGVLLVSIGLED"
     gene            1274..1519
                     /locus_tag="VLS86_00645"
     CDS             1274..1519
                     /locus_tag="VLS86_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HSO49025.1"
                     /translation="MCVAIPARVIAIGEPTAASIPGTVSMIDSERDIDLVMVPDARVG
                     DYVVVHSGYAIEVIPEQRATETMRLLGVEEGGRTDPL"
     gene            complement(1516..2193)
                     /locus_tag="VLS86_00650"
     CDS             complement(1516..2193)
                     /locus_tag="VLS86_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HSO49026.1"
                     /translation="MAVPGGVIVVGGGKVGRYLASLLQESGSMVRVIETRPARATELQ
                     SAGFDVVEGSGTDADVLERAGIRAGSSLVAVTGSDETNLAIASLARYEFGVERVIARV
                     VDPRNAWIYGPELGVDAAVNQADILAHLALEELSLGGMRILLKLRRGQYDLIEEEVQS
                     GAKVDGLLISDLRLPDRCVMVGVIRSGELESVGPDTRLQSGDHVLAVVHSDHIALLSS
                     LLGEPEG"
     gene            complement(2193..2843)
                     /locus_tag="VLS86_00655"
     CDS             complement(2193..2843)
                     /locus_tag="VLS86_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HSO49027.1"
                     /translation="MRIVVVGCGRVGSGVAGTLDGAGHSVTVLDRDPAAFSRLGRQFG
                     GQTVLGVGFDLEVLEKAEVSRADAIAVVTGSDEANAVISRLASTRFRVPRVVARMYDP
                     RQADLYRRLGVMTISPGSWGISRVVELVTLADLAITASLGAGQVDVMEGVVSVRLGGS
                     PVGELEIPGEAKVIAVTRGGRTRLMEELSVLEAGDVVHMVVAAGAAGRVQTLLRGE"
     gene            complement(2840..>5201)
                     /locus_tag="VLS86_00660"
     CDS             complement(2840..>5201)
                     /locus_tag="VLS86_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HSO49028.1"
                     /translation="GGLETSLRAEDLVPGDVLLLAEGDRISADARLVEAVALRVDQST
                     LTGESRSVRKSAEPYASDGRSPAELPCLVMAGTTVSSGRGRAVVCATGTHTEFGKVAA
                     LTGGMKEELSPLQVEMKRLSVMVSVVAVAAGMVFFVLALTLGEVPAARGLVFALGMIV
                     AFVPEGLLPTVTLALAMGTQRMATRNALVKRLSAVETLGSTTVICTDKTGTLTQNEMT
                     VRAAAVGPSVYQVGGVGYAPEGIIAPDPDEALLDLFRALALASNARLVAPGAEVDRWS
                     VVGDPTEGALLVAAVKAGVDLGALSMAAPRIAEFPFDTERKRMSAVNRIGAGSVLSVK
                     GAPSELLARCSRLADGRELDETLRAQIMTTVERFAVEGLRVLGAARRTIRESEVGGEA
                     DTVERDLEFLGLVAMHDPPRPEVKAAVETCKRAGIRIVMITGDYGVTAATIGRRIGVV
                     SGEGTRIVNGEELERMTDLELARALDGEVVFARATPELKLRVVTALQARGDVVAVTGD
                     GVNDAPALKKADIGVAMGLSGTDVAKEAADMILLDDNFASIVAAVEEGRAVYSNIRKF
                     TTYILTSNVPEAVPFVAFALSGGRIPLALDVMHILAIDLGTDLIPALGLGAEPPEPGV
                     MDKPPRAQTEHVIDKRLLRRAYLWLGPVQAAFVMLAFFIGFRLLGYDTSGDLPASGPD
                     YRAATAMALAAVVFTQIGNLFAQRSETESLRRVGLGGNRMLWWGVASELVLIGLIVYT
                     PVLNRIIGTAPFPAAGWLWLLLGIPVVPLADELRKSLSRRRARRKK"
BASE COUNT          924 a         1829 c         1622 g          826 t
ORIGIN      
        1 ctggatatcg gcgtcgacga gtggggtgac cacttcgagg agacccacgt ctcgtggtcg
       61 aacgccctcc acgcccgacg caaggggggt gggtcgtacc acctcggacc catggcccgt
      121 tactccctca actcgggaca gctgaccccg gtggccgccg aggctgccca cgaggccggt
      181 cttggcccca cctgtcacaa ccccttccgc agcatcgtcg tccgggcggt cgagatcgcc
      241 ttcgcctgcg aggaggcact gcgcctgatc gacggctaca cccagccccc gatcccggct
      301 atcgaggtcg agccacgggc cgtgacgggt catggggcgt cggaagcacc gcgcggcacc
      361 ctctaccacc gctacgcgat cgatcccgac gggctcatca ccgaggcgca catcgtcccc
      421 ccgacctcgc agaaccagct gagcatcgaa gacgacctcc gcgcctatgt ggtcggccgc
      481 gctcatctgc ccgatgacca gctccagtgg cagctggagc aggcgatccg ctgctatgac
      541 ccgtgcatct cttgcgccac ccacttcctc gacctgaccg tggatcgggg atgaggcgaa
      601 tcgtgatcgg cgtcgggaac ccggttcgtg gcgacgacgg cgcgggtctg gaagtcgccg
      661 gccgcttgcg ccgagtcgag acctaccagc gatccaccgg ctcctacgag ctgatcgatc
      721 tctgggacgg tgccgacgag gtgatcgtgg tcgacgcggc caggtcgggc gcgccggccg
      781 ggaccatcca tcgcttcgac gcccacgaaa ggccactgcc cgaaggggtc cttgccacca
      841 gcacccactc agtgggagtc atggagacca tcgagatggc gcgcagtctg ggccggctcc
      901 cggagcgagt ggtcgtgtac ggaatcgagg tctcgaccct gagtccggga tcgggtctct
      961 ccgacgtggt ggagcaggcg gtcaacgagt tggtgacggt gatcgacaat gcatgagagc
     1021 gggatcgtgc gcgacctgat gagcagggtc gaggaggaag ccgacgtcgg aaacggtcag
     1081 gtcaccaggc tgagattcag ggtcggggcc ctggccggaa tcggctcgga gtcactgatc
     1141 gagggtgcgc gccactacgc gatcgagaat tggggctacg cccccgaagt cctggtcgag
     1201 gaggcagcgg accccaccga ccccaacgcg ctcggtgtgc ttctggtgtc tatcgggctc
     1261 gaggattagc accatgtgcg tcgccatccc ggcacgggtc attgccatcg gagagcccac
     1321 cgcggcgtcg atcccgggaa cggtgtcgat gatcgactcg gagagggaca tcgatctggt
     1381 catggttccc gatgccaggg tcggggacta tgtggtggtc cattccgggt acgcgatcga
     1441 ggtcatcccc gagcagcggg ccaccgagac catgcggctg ttgggggtcg aagagggcgg
     1501 caggaccgac ccgctctagc cctcgggctc gccgagtaac gaggacagga gcgcgatgtg
     1561 atcggagtgg actacggcga gaacgtggtc gcccgactgg agacgggtgt ccgggcctac
     1621 cgactcgagc tcaccggacc ggatcacccc aaccatcacg caccggtccg gcagcctcag
     1681 gtcactgatc agcaagccgt cgaccttcgc ccctgactgc acctcttcct cgatcaggtc
     1741 gtattgaccc cgacgcagct tgagcaggat cctcatcccg cccagtgaca gctcttccaa
     1801 ggcgagatgg gccaggatgt cggcctggtt cacggcggcg tcgacaccca gctccggacc
     1861 gtagatccag gcattcctgg ggtcgaccac gcgagcgatc acccgctcca cgccgaactc
     1921 gtatcgagcc agcgatgcga tcgccaggtt ggtctcatcg gagccggtga cggcaacgag
     1981 agacgagccc gccctgatcc cggcgcgttc caggacgtcg gcatcggtcc ccgagccttc
     2041 gacgacgtcg aaccccgcgg attggagctc ggtcgctcgg gccgggcggg tttcgatgac
     2101 tctgaccatc gaccccgact cctgtaacag ggacgccaga tatcgaccca ctttgcctcc
     2161 gccgacgacg atcactcctc ctggcacagc catcactccc ccctcagcag ggtctgcaca
     2221 cgaccggcgg cgcccgcggc cacgaccatg tgaaccacat cacccgcctc gaggacactc
     2281 aactcctcca tgagccgggt cctcccaccg cgggtgactg cgatcacctt ggcctcgccc
     2341 gggatctcga gctcacccac cggcgaaccg ccgagtcgca ccgacaccac accttccatc
     2401 acgtcgacct gaccggcacc caggcttgcg gtgatcgcca ggtcggccag ggtgacgagt
     2461 tccacgaccc tgcttatccc ccaggaaccg ggcgagatgg tcatgactcc gagacggcgg
     2521 tagagatcgg cctgtctcgg gtcatacatc cgggccacca ccctggggac acggaatcga
     2581 gtcgacgcca gacgagagat caccgcattc gcctcatcgc tgccggtgac gaccgcaatc
     2641 gcgtcggcac gactcacttc cgccttctcc agcacctcca gatcaaagcc gacccccagc
     2701 accgtttgcc ctccgaactg tcgtccgaga cgggagaagg cggcaggatc acgatcgagg
     2761 actgtgaccg aatggcccgc cccatccagt gtgccggcga ctccggagcc gaccctcccg
     2821 cagcccacaa cgacgattct catttcttcc tccttgcccg ccgcctgctc aggctctttc
     2881 gcaattcgtc cgccaaaggc acaaccggga tcccgagcag gagccagagc cagcccgctg
     2941 cggggaaggg ggcagtgccg atgattcggt tcagcaccgg ggtgtagacg atcagcccga
     3001 tcagaaccag ctccgaggca accccccacc acagcatccg gttgccgcca agaccgactc
     3061 tcctgagtga ctcggtttcc gagcgctgcg cgaagagatt gccgatctgg gtgaacacca
     3121 ccgcggccag ggccatggcc gtggcggccc ggtagtcggg gccggatgcc ggcaggtcgc
     3181 cgctcgtgtc gtagcccagg agcctgaacc cgatgaaaaa ggccagcatc acgaacgcgg
     3241 cctgaaccgg gcccagccag agataggcgc gacggaggag acgcttgtcg atcacatgct
     3301 cggtctgtgc ccgcgggggc ttgtccatga cacccggctc tggcggctct gcacccaacc
     3361 cgagagccgg gatcaggtcg gttccgaggt cgatagcgag gatgtgcatc acatcgagtg
     3421 ccagggggat cctgccccct gacagggcaa aggcgacaaa gggcaccgct tcgggcacat
     3481 tggaggtgag gatgtaggtc gtgaactttc ggatgttgga gtagacggct cgaccctcct
     3541 ccacggcggc gacgatcgag gcgaagttgt catcgaggag gatcatgtcg gcggcctctt
     3601 tcgccacgtc ggtcccggag aggcccatcg caaccccgat gtctgccttc ttcagcgccg
     3661 gggcgtcgtt gacgccgtcc ccggtgaccg caacgacatc cccacgtgct tgcagggcgg
     3721 tgacgaccct cagcttgagc tctggcgtcg ccctggcgaa gacgacctcg ccatcgaggg
     3781 ccctagccag ctcgagatcg gtcatgcgct ccaactcttc gccgttgacg atcctcgtcc
     3841 cctccccgga caccaccccg atgcggcgcc cgatggtggc cgcggtgacg ccgtagtcgc
     3901 cggtgatcat cacgatccga atcccggccc gcttgcaggt ctccaccgcc gccttcacct
     3961 cggggcgggg tgggtcgtgc atggcgacca gacccagaaa ctcgagatcc ctctcgaccg
     4021 tgtctgcctc gcctccgacc tctgattcgc gaatggttcg tctcgctgcc ccgaggaccc
     4081 ggagaccctc gaccgcgaat ctctccaccg tcgtcatgat ctgggccctc agcgtctcat
     4141 cgagctcccg accgtccgcc aatcgggagc agcgagccag gagctcggac ggagcgccct
     4201 tcaccgagag aacagagccg gctccgatcc ggttgacggc gctcatgcgc tttcgctctg
     4261 tgtcgaacgg gaactccgcg atgcgcggag cagccatcga caacgccccg aggtcgacgc
     4321 ccgccttcac cgcggcaacc agcagcgccc cttcggttgg atctccgacc accgaccacc
     4381 ggtccacctc cgcgccggga gcgacgagtc gcgcgttcga ggcgagcgcc agcgctcgaa
     4441 acagatccaa cagtgcctcg tccggatcgg gcgcaatgat gccttccggt gcgtagccga
     4501 cgccgcccac ttggtagacc gaaggcccga cggctgccgc ccttaccgtc atctcgttct
     4561 gggtgagggt gcccgtcttg tcggtgcaga tcaccgtggt cgagcccagg gtctccactg
     4621 cggagagccg cttgacgagt gcgttcctgg ttgccattcg ctgtgttccc atggccaggg
     4681 caagtgtcac cgtggggagc aagccctcag gcacaaaggc cacgatcatg cccagagcga
     4741 agaccaagcc cctggccgcg gggacctctc ccagggtcag ggcaagcacg aagaacacca
     4801 tcccggctgc cacggccacc acactcacca tcacgctcag acgcttcatc tcgacctgga
     4861 gcgggctgag ctcctctttc atgccgccgg tgagcgcggc caccttgccg aattcggtgt
     4921 gggtcccggt cgcacagacc acggcccgac cccgcccgct ggaaaccgtg gttcccgcca
     4981 tcaccagaca gggcaactcc gccggagagc gcccgtcaga cgcatagggc tcagccgact
     5041 ttcgcaccga ccgggactca ccggtgagtg tcgattggtc gactcgcaac gccacggctt
     5101 cgaccaacct ggcgtcggcg gagatgcggt cgccctcggc gagaagcagc acgtctcccg
     5161 gaaccaggtc ctccgctctc agagaagtct cgagcccgcc a
//
LOCUS       DATITW010000053         1502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_122681, whole genome shotgun sequence.
ACCESSION   DATITW010000053 DATITW010000000
VERSION     DATITW010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122681"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..699)
                     /gene="extP"
                     /locus_tag="VLS86_00665"
     CDS             complement(10..699)
                     /gene="extP"
                     /locus_tag="VLS86_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HSO49029.1"
                     /translation="MLRPGSPFKKGYSDSPRNRSYVVMNNVLYHLHPVKVKRHGVKLS
                     YTLCLGGLSFFLFILLTITGIFLMFYYTPSAETAYADIEALSTNVAFGSLVRNMHRWG
                     AHLLVLSVFLHMSRVFYHGAYKPPREFNWVVGVVLLLLTLLLSFSGYLLPWDQLALWA
                     VTVGTNMAGFVPVVGAQVKFALLAGVEVSAATLLRFYVLHVLAFPFIIVLFMAVHFWR
                     VRKDGGISGPL"
     gene            complement(795..1244)
                     /locus_tag="VLS86_00670"
     CDS             complement(795..1244)
                     /locus_tag="VLS86_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HSO49030.1"
                     /translation="MPKGVVDETPPYRDDYQLAMVGNDYFADKLRPKQFLHIDQSECI
                     ICEGCVDICPWKCIHFLSLAAISEGVNVDHPGEDPQNLGFFVVDEDECTRCALCIDRC
                     PTNVITLGKFEGSLADQAAELGLDRRPWDLDTGQRDFKNGYAYGMRW"
     gene            complement(1244..>1502)
                     /locus_tag="VLS86_00675"
     CDS             complement(1244..>1502)
                     /locus_tag="VLS86_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49031.1"
                     /translation="AQTDLNWSSQRVALVIPPVLVLGNDVEISWEVVKDSISMGYHIV
                     MLVAAAVFALQLQKMKQGAPASAEKPQARSPYGRPLIKGGA"
BASE COUNT          300 a          454 c          492 g          256 t
ORIGIN      
        1 ggatgtctcc tacagcggtc ccgagattcc gccgtccttg cggactctcc agaaatggac
       61 ggccatgaac aggacgatga tgaacgggaa ggcgagcacg tggaggacgt agaagcggag
      121 cagggtggcg gcggaaacct cgaccccggc cagcagggcg aacttcacct gggccccgac
      181 caccggcacg aaaccggcca tgttggtccc gacggtgacc gcccacaggg cgagctggtc
      241 ccaggggagc agatagccgg agaacgacag aagcagggtc agcagcagca gcaccacgcc
      301 gaccacccag ttgaactcgc gcggcggctt gtacgccccg tgatagaaga cccggctcat
      361 gtgcaggaac accgacagca ccaggaggtg ggcgccccag cggtgcatgt tcctcaccaa
      421 cgacccgaag gcgacgttgg tggagagggc ctcgatgtcg gcgtaggcgg tctccgccga
      481 cggcgtgtag tagaacatta ggaagatgcc ggtgatggtg aggaggatga agaggaagaa
      541 gctgagacct ccgagacaaa gggtgtagga cagcttcacc ccatgacgct tcaccttgac
      601 cgggtggagg tgatagagga cgttgttcat caccacgtag gagcggttcc tggggctgtc
      661 cgagtagccc ttcttgaagg gcgagccggg acgcaggatc gactcggcga cctgagaggt
      721 ccataccttc tcattcacct ctttgatcct gtccaccaga ccggtcttgc cgtttccgtt
      781 agccaatccg cctcctacca gcgcatgccg tatgcgtagc cgttcttgaa gtcacgctgt
      841 ccggtgtcga ggtcccaggg gcggcgatcg agaccgagct ccgccgcctg gtcggccaac
      901 gagccttcga acttgcccag cgtgatgaca ttggtcgggc accgatcgat gcagagggcg
      961 cacctggtgc actcgtcctc gtcgacgacg aagaagccga gattctgcgg gtcttccccc
     1021 gggtggtcga cgttgacccc ttccgatatg gcggccagtg agaggaagtg gatgcacttc
     1081 caggggcaga tatcgacaca gccctcgcag atgatgcact ccgactggtc gatgtggagg
     1141 aattgcttgg gtcgcagctt gtcggcgaag tagtcgttgc ccaccatcgc cagctgatag
     1201 tcgtcacggt atgggggagt ctcgtcgacg acgcccttgg gcatcaggcg ccgcccttga
     1261 tcagcggccg cccgtacggg gagcgagcct gtggcttctc ggcggaggcc ggggcgccct
     1321 gcttcatctt ctgcagctgg agggcgaaca ccgcagccgc aaccagcatc acgatgtggt
     1381 agcccatcga gatcgagtcc ttcaccactt cccagctgat ctcgacatca ttgcccagga
     1441 ccagcaccgg gggaatcacc agggccaccc gctgcgagga ccagttgaga tcggtctggg
     1501 cg
//
LOCUS       DATITW010000054         1544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_42647, whole genome shotgun sequence.
ACCESSION   DATITW010000054 DATITW010000000
VERSION     DATITW010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42647"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VLS86_00680"
     CDS             <1..257
                     /locus_tag="VLS86_00680"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HSO49032.1"
                     /translation="RARSSALRIGSFLTHPASTAEVFAYRRESDDETMTIALNFGQQK
                     TTISLGACTVVFSTADPERSDRANQGLRLAPLEGVVVSHV"
     gene            329..>1544
                     /gene="ugpC"
                     /locus_tag="VLS86_00685"
     CDS             329..>1544
                     /gene="ugpC"
                     /locus_tag="VLS86_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HSO49033.1"
                     /translation="MARINMEGLGKVYEDGTRAVGNLDLEINDGEFLVLVGPSGCGKS
                     TVLRMIAGLEDITEGTMRIGDKVVNDVAPKDRDIAMVFQNYALYPHMSVFDNMAFGLK
                     LRKFKKEEILRRVQEAADILEIGEYLQRKPKALSGGQRQRVAMGRAIVREPAAFLMDE
                     PLSNLDAKLRVQMRAEVERLHSKLATTTVYVTHDQTEAMTLGDRVAVLRPVSAARPFN
                     LQQVGPPTELFDRPDNLFVAGFIGSPAMNLSYGHLENEGDQIWAVTTGGFRVKVDQRA
                     LASHPGIEKKMNQELVVGIRPNSLEAAQVAGADPDRTIRAHVEVTEMLGAETFVHFTV
                     NRPPVMTPDIEELLADTGQDPSSLGETTNFIARVSPDAQIKRGDELDLIVDCAKLHFF
                     DPKTGLRIGSELK"
BASE COUNT          308 a          484 c          476 g          276 t
ORIGIN      
        1 ttcgtgcccg atcctcggcg ctccgcatcg gttcgttcct cacccatccc gcgtccactg
       61 ccgaggtgtt tgcctatcgg cgcgagtccg acgacgagac aatgaccatc gccctcaact
      121 tcggccaaca gaagacgacg atctcacttg gagcgtgcac cgtcgtcttc tccaccgccg
      181 acccggaacg atcggaccga gccaaccagg ggctacgtct ggcaccgctc gaaggggtgg
      241 tggtcagcca tgtatgacac cacccgacca cacctggtgt tttccgctag tttcatttct
      301 cagccgacga gggaacggag aatctgagat ggctcgcatc aacatggaag ggctggggaa
      361 ggtctacgag gacggcacga gggccgtcgg gaacctcgac ctcgagatca acgacggcga
      421 gttccttgtg ctcgtcgggc cttcgggatg cggcaagtcg accgtcctgc ggatgatcgc
      481 cggtctcgag gacatcaccg agggcacgat gaggatcggt gacaaggtgg tcaacgatgt
      541 cgctcccaag gaccgtgaca ttgcgatggt cttccagaac tacgccctct acccccacat
      601 gtcggtattc gacaacatgg ctttcggtct caagctgaga aagttcaaga aagaggagat
      661 tctgcgccgg gtgcaggaag ccgccgacat cctcgagata ggtgagtacc tccagcgcaa
      721 gccaaaggcg ctctctggcg ggcagcggca gcgggtggcg atggggcggg ccatcgtccg
      781 cgagccggcg gcgttcctca tggacgaacc tctctccaat ctcgacgcca agctcagggt
      841 gcagatgcgc gccgaggtgg agcggctcca ctcgaagttg gcgacgacca cggtctatgt
      901 gacccatgac cagaccgagg cgatgacgct cggtgatcgc gttgccgtcc tgcggcctgt
      961 ctccgcggcg cgtccgttca acctgcagca ggtcgggcct cccaccgagc tgttcgatag
     1021 gcccgacaat ctctttgtcg ccggtttcat cggaagcccg gcgatgaacc tctcgtacgg
     1081 gcatttggag aacgagggcg atcaaatctg ggcggtgacg accggggggt tcagggtcaa
     1141 ggtggaccag cgcgccctcg cctcacatcc cggcattgag aagaagatga accaggaatt
     1201 ggtggtcggc atcagaccca actcgctgga agcagcacag gtcgccgggg ccgatccaga
     1261 tcgcacgatt cgcgcccacg tggaagtcac ggagatgctc ggcgcggaga catttgtgca
     1321 tttcacggtg aaccggccgc cggtgatgac cccggacatc gaagagctcc tcgctgacac
     1381 cggtcaggac ccgtcctcgc ttggtgaaac caccaacttc atcgcacgtg tcagccccga
     1441 cgcccagatc aagcgcgggg acgagctgga tctgatcgtc gattgcgcca agctccactt
     1501 cttcgacccg aagactggtc tccgcatcgg atccgagctc aagg
//
LOCUS       DATITW010000055         1506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_122730, whole genome shotgun sequence.
ACCESSION   DATITW010000055 DATITW010000000
VERSION     DATITW010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122730"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VLS86_00690"
     CDS             complement(<1..863)
                     /locus_tag="VLS86_00690"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HSO49034.1"
                     /translation="MVVDSLLNGGRIVNLVSSFQRMGAVLALLLLTACAAGVTEATGQ
                     SSTTSGAPDQASTVTSAPDRTTTTGSADAQLEGNRPASIDVPAGYDPAVPVPLLVVLA
                     SFGTPGAEELTYTGLGDLVAEEGILVVAPDGSLNDDGEHFWNATDACCADVGSDVDDV
                     AYLGSLIEEISAEWNVDPARVFAFGHSNGGFMAYRLACDRADLGAAVVSVAGVTWSDP
                     TACDPSHAVSVLQIHGTDDEVVRYEGGRFAVPYPGGMETVAAWATYDSCTGGLVTDPA
                     QIDIDENVAGI"
     gene            936..1334
                     /locus_tag="VLS86_00695"
     CDS             936..1334
                     /locus_tag="VLS86_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49035.1"
                     /translation="MAEIVVGGIQVAVGAKRVRQAANVCQHTETLITATAGLERIVCE
                     TCGHLSFNFPDVLSGPVHRRWFARPADRVEEARVVDEPDDDWLTQYTPVTHTPHVDEE
                     AAAARAFAAQERFHIRSRYELHPEPSVAVA"
BASE COUNT          289 a          500 c          468 g          249 t
ORIGIN      
        1 tcgatcccgg ccacgttctc gtcgatgtcg atctgggccg gatcggtgac gaggccgccg
       61 gtgcagctgt cataagtggc ccacgcggcg acagtctcca tcccccccgg ataaggcacc
      121 gcgaaccggc cgccctcata gcgaacgacc tcgtcatccg tgccgtggat ctggagcacg
      181 ctcacagcgt gtgagggatc gcatgccgtc gggtcgctcc aggtgacgcc cgcgacgctg
      241 acgaccgcag cccccaggtc ggcacggtca caggccaacc gatatgccat gaagcctccg
      301 ttggagtgcc cgaacgcgaa cacccgagcc gggtcgacat tccattccgc ggagatctcc
      361 tcgatcagag agccgaggta ggcgacgtcg tcgacgtctg acccgacatc ggcgcagcac
      421 gcgtcggtgg cgttccagaa atgctcaccg tcgtcgttca aggaaccgtc gggtgcgacg
      481 accaggatgc cctcctcggc gaccagatcc ccgagcccgg tataggtcag ttcctccgcc
      541 ccaggtgtgc cgaatgaagc cagcaccacc agcaacggca ccgggaccgc cgggtcatag
      601 cccgcaggga cgtcgatgct ggccgggcgg ttgccctcga gttgagcgtc cgcggacccg
      661 gtcgtggtgg tccggtccgg cgcactggtg acagtggagg cctgatccgg cgctccgctc
      721 gtggtgctgg attgacccgt cgcctcggtg actccggcgg cgcaagctgt cagcagcaac
      781 aaggccaaca cggcacccat ccgctggaag ctagatacca ggttcacgat gcgcccacca
      841 ttcagcaggc tgtcgacaac cacagcacga cttatccaca gtttgggtct tctgacccat
      901 atcggattgc caccgctttc gatacaccac gtgctatggc ggagattgtt gtgggaggca
      961 ttcaagtcgc tgtcggcgcc aagcgggtgc gacaggcggc caacgtctgc cagcacaccg
     1021 agacgctgat caccgcaacc gcaggcctgg aacgaatcgt ctgtgagacc tgtggtcatc
     1081 tcagcttcaa tttcccggac gttctctccg ggcccgtaca ccgcagatgg ttcgctcgcc
     1141 ccgcagatcg ggtcgaggag gctcgggtgg tcgatgaacc ggacgacgat tggctaacgc
     1201 agtacacacc ggtgacccat actccccatg tcgatgagga agccgcggcc gcccgagcct
     1261 tcgcggccca agagcggttc cacatccgga gccgctacga gctgcatccc gaaccgtcgg
     1321 tggcagtcgc ctagatcgac cggaaacagt cgaccgactg aggacaccgg tcaggcgata
     1381 gccgtactct tcgaccagga gagaacatcc cagcttgtag aggaactggc atgagtctca
     1441 cggagatcga agccgacacc gccgtcgcct ttgtcaggag cttcgtgtag acggagggca
     1501 ggagcg
//
LOCUS       DATITW010000056         1550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_763712, whole genome shotgun sequence.
ACCESSION   DATITW010000056 DATITW010000000
VERSION     DATITW010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763712"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VLS86_00700"
     CDS             complement(<1..223)
                     /locus_tag="VLS86_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014695275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HSO49036.1"
                     /translation="MTQLVAVGAHVDPRSPVAEARARGADLVQIFLSDPQSWHKPAPR
                     PDAAELAASPIPIYVHAPYRINVVAEASRI"
     gene            complement(220..885)
                     /locus_tag="VLS86_00705"
     CDS             complement(220..885)
                     /locus_tag="VLS86_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49037.1"
                     /translation="MMEEIRVSRSGIRMWLIALAGVPMVVIGVDVLLRRRIFGVFSSL
                     VFTGDPPPVEPRDVIWAVVLLILGLIFIGFGLRELLVPSPVLVADAFGLHLHLGHPLS
                     RLVTIPWSEVDDIGAEDLDDDGSVIPVMWVRGLNPEQLPANPWGARWIEPDTLAVMAA
                     DWERTPRFVAETAAEIAVSAAQVPDLPVVESAAERMAREALFGSPPGPGAVAGSTDTE
                     VIT"
     gene            complement(882..1418)
                     /gene="pyrE"
                     /locus_tag="VLS86_00710"
     CDS             complement(882..1418)
                     /gene="pyrE"
                     /locus_tag="VLS86_00710"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HSO49038.1"
                     /translation="MEALIAHLRKNALRTDGPFVLRSGATSDWYLDARQTTYSGEGAR
                     LVGEAILKVLHESVVAVGGMTMGADPVAMAVALMSADGHRALRAFSIRTEAKDHGTGG
                     RLVGPIAAGDTVAVVEDTVTTGSAMLEAIDVLTEAGIDVVQAISVVDRSGGAAGGRIG
                     QRGIPFVALLRPADLGVE"
BASE COUNT          304 a          525 c          472 g          249 t
ORIGIN      
        1 ggatacgaga ggcctcggct acgacattga tccgataagg cgcatgcacg tagatcggta
       61 tgggtgacgc agccagctcg gcggcgtcgg ggcgaggagc cggcttgtgc cacgattgcg
      121 ggtcggacag aaagatctga acgagatctg ctcctcgagc ccgcgcctcg gcgacgggcg
      181 acctgggatc tacatgggcc ccgaccgcca caagctgagt cacgtgatca cctcggtgtc
      241 ggtcgaccct gccactgcgc ccggtcctgg tggcgaccca aacagcgctt ccctggccat
      301 gcgctcagcc gcggattcga caaccggtaa gtcgggaacc tgtgcggcgc tgacggcgat
      361 ttcggccgca gtctcggcca cgaaccgcgg ggtccgctcc caatcggccg ccatcaccgc
      421 cagcgtgtcc ggctcgatcc agcgggcgcc ccacgggttg gcgggaagtt gctcggggtt
      481 caaccctctc acccacatca cggggatcac cgacccgtcg tcgtcgaggt cttcggcccc
      541 gatgtcgtcg acctcggacc acgggatcgt caccaatcgt gaaaggggat gtccaaggtg
      601 cagatggagc ccgaaggcat cagccaccaa gacggggctt ggtaccaaaa gctcgcggag
      661 tccgaatccg atgaagatga gaccgaggat caggaggacc accgcccaga tcacatctcg
      721 gggttcgacc gggggcgggt cgcccgtgaa cacgagagaa ctgaagactc cgaagatgcg
      781 acgtcgcagc aggacgtcga cgccgatgac caccatcggc acgcctgcga gcgcgatcag
      841 ccacatccga atgccggatc ggctgactct gatctcctcc atcattcgac tcccagatcc
      901 gccgggcgaa gcaacgccac aaatggtatg ccccgctggc cgatacgccc acccgccgca
      961 ccgccactgc gatcgacaac gctgattgcc tggaccacgt cgatccccgc ctcggtcaac
     1021 acatcgatcg cttccaacat ggcagagccg gtggtgaccg tgtcctcgac caccgccact
     1081 gtgtctccag cagcgatggg accgacgaga cgaccgccag ttccgtggtc cttggcttct
     1141 gttcggatcg aaaaggcacg cagagcgcgg tgaccatcgg cactcatcaa cgccacggcc
     1201 atcgcgaccg ggtcggcccc catcgtcatt cccccgacgg cgaccaccga ctcatggaga
     1261 accttcaaga tggcctcccc gaccaatcga gccccttccc ccgagtaggt ggtctggcga
     1321 gcgtcgagat accagtccga cgtggcaccg gatctcagga cgaacgggcc gtcggttctg
     1381 agcgcattct ttcgaaggtg ggcgatcagg gcttccatcc gggcttcaca gtcgattgga
     1441 gggctaaacc attggacgag acatgccgat aaatgcacga agttcacgag ggttccttct
     1501 ccccccgtgg tctgtcccga cagtagtggc cgcccgtaac ccgggcggcc
//
LOCUS       DATITW010000057         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_323050, whole genome shotgun sequence.
ACCESSION   DATITW010000057 DATITW010000000
VERSION     DATITW010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323050"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VLS86_00715"
     CDS             complement(<1..946)
                     /locus_tag="VLS86_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HSO49039.1"
                     /translation="MYETQQLLRQFEKRAYDLFLQNLVKGTSHLSLGMEAVAAGFGHA
                     MRGDDYSFATYRGHAHTLARGAAPGPVLAELLGRENGILKGKGGSMHLTSVEHGMMGS
                     YAIIGAHLTIANGAAWSAQMRGTDQVTVCFFGDGTTNIGAFHEALNFAAIWELPVVFV
                     CENNLYMEYTPIDLITAVPNPAADRAGAYGLESIIVDGNDADAVFDVAKRVLGRARDG
                     GGPALVEAQTYREQGHSRADPGKYRPPAEIEAWKARDPIPMYHERLLRLGVPEGDLSS
                     IQQDVARRVDEATEFAKGGREPGREHLMTDVWADGGSAW"
     gene            complement(1172..>2386)
                     /locus_tag="VLS86_00720"
     CDS             complement(1172..>2386)
                     /locus_tag="VLS86_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012808064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO49040.1"
                     /translation="VDGVSFDVERGQTLGLVGESGCGKTTLGRVVSGLSAPTGGGVYF
                     DVDPLTRARLDDLLAVPEPDRTATQTTELATIESAHRVDRLEGAAWKRFRRNCQVVFQ
                     DSFASLNPRHLVRDIVGRPLKVQREASGGELTERVVELLEQVGMGRQHLYRYPHQFSG
                     GQRQRISIARALALDPELLVLDEPTSALDVSVQAQILNLLHGLQVQRNLTYLFITHDL
                     SVVRHMADRIVVMYVGKVAEAGPTEVIFDAPQHPYTRALLGASPDIDDDRSQFRGLEG
                     SVPDPARPPQGCRFHTRCPVATPVCGWEVDDVVLRLQDEPGLFDQVDAVEHRTDFDAD
                     IRFADPTGAQRLASLLRSQRVPGAMSDAIEQISVSDRTVTLRFRPVEEVRLTQRGPDH
                     VAACVLGERLSP"
BASE COUNT          408 a          805 c          777 g          396 t
ORIGIN      
        1 gccacgccga ccccccgtcc gcccagacgt cggtcatcag gtgctcacgc cccggctcgc
       61 ggccaccctt ggcaaactcc gtcgcctcgt ccactctcct ggcgacgtcc tgttggatcg
      121 agctcagatc accttcgggc acaccgagac gcagcagacg ctcgtggtac atgggaatcg
      181 ggtcccgggc cttccaggcc tcgatctcgg cgggaggccg gtacttgccg ggatcggccc
      241 ggctgtggcc ctgctcccga taggtctggg cctcgaccag cgccgggcct ccgccatccc
      301 gggcccgtcc caggacccgc ttcgcgacgt cgaagaccgc atccgcatcg ttcccgtcga
      361 cgatgatgct ctcgaggcca taggcccctg cccggtcggc agcagggttg ggcaccgcgg
      421 tgatcaggtc gatcggcgtg tactccatgt acagattgtt ctcacacacg aacaccaccg
      481 ggagctccca gatggcggcg aagttgagcg cctcgtgaaa ggccccgatg ttggtcgtcc
      541 catcgccgaa gaagcagacg gtcacctgat cggtcccccg catctgcgcc gaccacgcgg
      601 ctccattggc gatggtcaga tgggcaccga tgatggcgta ggagcccatc atgccgtgct
      661 cgacagaggt caggtgcatc gagccgccct tgcccttgag gatcccgttc tcccggccca
      721 ggagctcggc cagcaccggg cccggcgccg cgccccgggc cagggtgtga gcgtggccgc
      781 gataggtggc gaagctgtag tcgtctcctc gcatagcatg gccgaaaccg gcggccaccg
      841 cctccatgcc gagcgagaga tgagaggtcc ccttgaccag gttctggagg aagaggtcgt
      901 aggcccgctt ctcgaactgc cggagaagct gctgggtctc gtacatctcg agacgcagat
      961 cgatcggaat ctccagacca tcgttgtcac ggttctcggg ctccgggagc cgtggctcgc
     1021 gcaaggcgta gtcgtagagc ccccgatcct ggtcggcact catgtcagtg gtctcctctc
     1081 acgccacaaa gctaacggct cacaacgcat cagccgaaga agaagggatg gcgatggcga
     1141 tgcatgaccg gtcacgagac ggtggcgggg ttcagggcga cagccgctct ccgagcacac
     1201 aggccgcgac atggtccggc ccgcgttgag tgagccggac ctcctccacg gggcggaatc
     1261 tcagagtgac cgtcctgtca ctcaccgaga tctgctcgat cgcatcactc atggcaccgg
     1321 gaaccctttg cgaccggagc agggaggcga gccgctgggc gccggtcggg tcggcaaacc
     1381 ggatgtcggc gtcgaagtcg gtgcggtgct ccaccgcgtc cacctggtcg aagagacccg
     1441 gctcatcctg cagtcggagc acgacgtcgt cgacctccca accgcacacc ggcgtcgcca
     1501 ccgggcaacg ggtgtggaag cgacatccct gtgggggtcg agctggatcg gggacactcc
     1561 cctccaatcc ccgaaattgg gatcggtcgt cgtcgatgtc ggggctggcg ccgagcaggg
     1621 ccctggtgta cgggtgctgg ggggcatcga atatgacttc tgtcggaccg gcctccgcca
     1681 ccttgccgac gtacatcacc actatccggt ccgccatgtg gcggacgacc gaaaggtcgt
     1741 gggtgatgaa cagataggtg aggtttcgct gcacctggag accatggaga aggttgagga
     1801 tctgggcctg aaccgagacg tcgagggcac tggtcggctc gtcgaggacc agcagctccg
     1861 ggtcgagggc cagggcccgc gcgatcgata tccgctgacg ctggccaccg gagaactggt
     1921 gtgggtaccg atagagatgc tgacgtccca tgcccacctg ctccagcagc tcgacgactc
     1981 gctcggtcaa ctctcctccg gacgcctcgc gctggacctt gagcggacgg ccgacgatgt
     2041 cccggaccag atgccgcggg ttgagcgagg cgaaggagtc ctggaacacc acctggcagt
     2101 tgcgccggaa acgcttccat gctgcccctt ccaaccgatc cacacgatgc gccgattcga
     2161 tcgtggcgag ctcggtcgtc tgtgtcgcgg ttcggtcggg ctcggggacg gcgagcaggt
     2221 cgtcgagtct cgccctggtg agcgggtcga cgtcgaagta caccccacca ccggtgggag
     2281 ccgagagacc gctcaccacc cgtcccagtg tggtcttgcc gcacccactc tcaccgacca
     2341 ggcccagggt ctgaccgcgc tcgacatcga agctcacccc gtcgac
//
LOCUS       DATITW010000058         1673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_643489, whole genome shotgun sequence.
ACCESSION   DATITW010000058 DATITW010000000
VERSION     DATITW010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643489"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VLS86_00725"
     CDS             complement(<1..648)
                     /locus_tag="VLS86_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HSO49041.1"
                     /translation="MIPNTTRKPRDVESAVALLRPARRILVFTGAGISTESGIPDFRG
                     PDGLWSKVDPDDFHIDRYRSSPELRMRGWKMHLDGELWGARSTVRPNTGHEAIKRLAD
                     AGRLAGVVTQNVDGLHLVSGLDERQVAELHGNVRKSLCVECGQVWDTEIVLAWVETGE
                     LDPACPDCGGIVKTATVMFGEILPQEEMEKAMEFLAVSDAIIVVGSTVSVSPASDI"
     gene            724..1107
                     /locus_tag="VLS86_00730"
     CDS             724..1107
                     /locus_tag="VLS86_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSO49042.1"
                     /translation="MTTSEVQAWLDRYIEAWQKNDPELVEALFTSDAVYRFRPYGGEG
                     RVAEGIPDIVNSWIDFDEDPAEWEASYRPYAVDGDRAVAVGTSRYFGAGDEADEVYHN
                     CFLLRFADGRCAEFTEYWMLEPSEG"
     gene            1114..>1673
                     /locus_tag="VLS86_00735"
     CDS             1114..>1673
                     /locus_tag="VLS86_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HSO49043.1"
                     /translation="MSDPQSEMMRKGWDPEEGLDSLKTPLFHTSTFVFESSAAAERLF
                     AIVYGGESPEPGEDVGFIYTRMDHPNLVIVESRLAVWEGAEAALLFSSGTSAVFTTFL
                     THVRPGGLILHSTPLYGGTNTMLYKVLAPLGFELASYGPDASQDEIEDVIGDRHLAAV
                     YLETPANPSNDVFDIDLAARVAAAATT"
BASE COUNT          295 a          537 c          527 g          314 t
ORIGIN      
        1 gatgtcggac gccggcgaca ctgacaccgt cgatccgacc acgatgatgg cgtcggacac
       61 ggcgaggaac tccattgcct tctccatctc ctcctggggc aggatctccc cgaacatcac
      121 cgtggccgtc ttgacgatgc ctccgcagtc cgggcaggcg gggtcgagct cgccggtctc
      181 cacccaggca agaacgatct ccgtgtccca aacctgcccg cactccacgc aaaggctctt
      241 tctcacgttt ccgtgcaatt cggccacttg gcgctcgtca agtcctgaga ccaggtggag
      301 gccgtcgacg ttctgggtga ccactccggc cagccggcca gcgtcggcga gtctcttgat
      361 ggcttcgtga ccggtgttgg gtcgtaccgt ggagcgggcg ccccaaagct cgccgtcgag
      421 gtgcatcttc cagcccctca ttctgagctc gggactcgac cgatagcgat cgatatggaa
      481 gtcgtcgggg tcgactttgc tccacaagcc atcaggcccg cggaaatcgg gaattcccga
      541 ctcggtcgag atcccggcgc cggtgaaaac aaggatcctt cgtgctgggc gcaggagagc
      601 cacggccgat tcgacatcgc gaggcttgcg ggtcgtgttg ggaatcatcc ggatacgcac
      661 ctgtagctca actctacggc gaattcggtg tgagccggca agagcccggc gtacgatgcg
      721 cttgtgacga cctccgaagt ccaggcctgg ctcgatcgtt acatcgaggc gtggcagaag
      781 aacgaccccg agttggtcga ggccttgttc accagcgatg ccgtctaccg cttccggccg
      841 tatggcggcg agggccgcgt cgccgaaggt attcccgaca tcgtgaacag ctggatcgac
      901 ttcgacgagg acccggctga gtgggaggcg tcgtaccggc cctacgccgt cgatggcgat
      961 cgcgccgttg ctgtcggtac cagtcggtat ttcggtgccg gggacgaggc cgacgaggtg
     1021 tatcacaatt gcttcttgct ccggttcgcc gacggccgtt gcgccgagtt cactgagtac
     1081 tggatgctcg agccgagcga gggctgaacc gggatgtccg atccccaatc ggagatgatg
     1141 cgcaagggct gggatcccga ggaagggctc gactcgctca agacccccct cttccacacc
     1201 tccaccttcg tcttcgagtc ctcggccgcc gccgaacgtc tcttcgccat cgtctatgga
     1261 ggggagtctc cggagccggg cgaggacgtg gggttcatct acacgcgaat ggaccacccg
     1321 aacctggtca tcgtcgagag ccggctcgcc gtgtgggagg gggcggaggc agcgctccta
     1381 ttctccagcg ggacctccgc cgtgttcacc acattcctga cccacgtcag acccggcggt
     1441 ctgatcctgc acagcacacc cctttacggg ggtacgaaca ccatgctcta caaggtgctg
     1501 gccccacttg gattcgagct ggcgtcatat ggcccggacg catcccagga tgagatcgag
     1561 gacgtgattg gggatcgtca ccttgcggcc gtgtacttgg agaccccggc caacccgagc
     1621 aacgacgtgt tcgacatcga tttggcggca agggtcgcgg ccgcagcaac gac
//
LOCUS       DATITW010000059         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_763744, whole genome shotgun sequence.
ACCESSION   DATITW010000059 DATITW010000000
VERSION     DATITW010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763744"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..453)
                     /locus_tag="VLS86_00740"
     CDS             complement(184..453)
                     /locus_tag="VLS86_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49044.1"
                     /translation="MIAREDLETMTSRDLHDRAIGTAKAEGDLDWLWHLLRSIPAAEG
                     EVGDLEDSGMDMVSTVSAINGYTRADRDLPGTLRSQYVDYLLEHL"
     gene            625..906
                     /locus_tag="VLS86_00745"
     CDS             625..906
                     /locus_tag="VLS86_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49045.1"
                     /translation="MIRQPRGETSVSVPSAIDSTAPILAHHEVEIQAPLDEVWRLHTD
                     VNAWTTWQSDITTASIEGAFGVGNSFGWSSYGLAVTSTIYAITDHSRIL"
     gene            complement(1503..1961)
                     /locus_tag="VLS86_00750"
     CDS             complement(1503..1961)
                     /locus_tag="VLS86_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HSO49046.1"
                     /translation="MTDVSLDELGPVDYIVVEFPAGASNFTGEMAAELLALVDAGTIR
                     VIDVLILTKNEDGSVEAMELSDVSELGALQAVEAQLAELLAEEDVEHLAAAIEPGSTA
                     GVLIWENLWAAPFASAARRSGGQLIANGRIPIQAIIASIEADEAPTTQGD"
BASE COUNT          511 a          912 c          918 g          552 t
ORIGIN      
        1 tcttgagctg cggaaataag agtttgcgca cccttcacgc gttgactgct ccttcgtcgc
       61 gaaagagaca tagacatgga ctccacctcg gcaacgctct gacctcgggg aggctgaagg
      121 tcatcgacgc ctcgctagcc gggcggggct gcctgatcga gtttcttgta ccccacggcg
      181 tcgctacagg tgctccagga ggtagtcgac gtactgcgat cgaagggtgc cggggaggtc
      241 gcggtcggcc cgagtgtagc cgttgatggc ggagaccgtg ctgaccatgt ccatgcccga
      301 gtcctcgagg tctccgacct cgccctcggc cgcggggatc gagcgcagga ggtgccaaag
      361 ccaatcgagg tcgccttccg ccttggccgt gccgatggct cggtcgtgca ggtcgcggga
      421 ggtcatggtc tcgaggtcct cgcgggcgat cacggtgcaa ccctaccccg cggtggtggc
      481 gaccacccag catgggcttg cgtctcgacc ggccctaacc gcaactgcgt acacgagctc
      541 cggcctcgtt ctcgctcagc accccgaagg agggaggaag ggccgctcgc acccacagga
      601 gggcgaggag tacgattccg cgcgttgatc cgacaacctc ggggggagac atcggtgtcg
      661 gtcccgtccg cgatagacag cacagctccg atcctcgccc atcatgaagt tgagattcag
      721 gcgccattgg atgaggtctg gcggcttcac accgatgtca acgcttggac gacgtggcag
      781 tccgacatca ccaccgccag catcgagggc gcattcgggg tcggcaattc gttcggatgg
      841 agcagctacg gacttgccgt cacatcgacg atctacgcca tcactgatca ctcgcgaatc
      901 ctctagggcg ggaccagcga tgggatcacg ggtatccacg agtggctctt ttccgagacc
      961 ccggacggtg ttcgtgtgac gacgaatgag tcgttcgcgg gccagccggt tgaagcacac
     1021 gcgaccaaca tgcagtcact gctcgacgcg tcgctcgtgt cctggcttga tcacttgaag
     1081 gaggcagccg agtccggctc cgtctgacgc cagagggaag gcccgttcga cctcgccgcg
     1141 caccatccac gtccgccgac aaggctcctt attcgtacac caagacggcg atgaactgag
     1201 gcctcatgcg ccggggcgca agtcccagtt tggcaaaaga gctagacgaa ccagcggtcg
     1261 agaaggctcg ggcggtcggg accgtgagtc tcgctcgcga tgtttgcgcc tgcgttccga
     1321 gttcaggcag gctggctaga ttcgccgacg acgccgccca ggtgtgacgg ccgcggcaac
     1381 gaccgcggtt ttcgctacgg gcgaggggcc aggtgtgacg gccttggcca cgaccgcggt
     1441 cttcgccacc ggggcgccga tgacaccaac tcttcctgca cgtgctggcc tgagtggcat
     1501 gctcagtctc cttgggttgt aggtgcttca tcggcctcga tggaggcgat gatcgcctgg
     1561 atcgggatcc gtccgttcgc gatcagttgg ccaccagaac gccgcgccgc tgaggcgaat
     1621 ggcgcagccc acaggttctc ccagatcagc actccagcag tgctccctgg ttctatggcg
     1681 gcggccaggt gctcgacgtc ttcctcggcc aacagctccg ccagctgcgc ctcgactgct
     1741 tggagcgctc cgagctcgga gacgtcggat agctccatcg cctcgacgga accgtcctcg
     1801 ttcttggtca ggatcaacac gtcgatcacg cggatcgttc cggcgtcaac caaggcgagc
     1861 agttctgccg ccatctcgcc cgtgaagttg ctcgctcctg ccggaaactc gacgacgatg
     1921 tagtcaacgg ggccgagttc atctagggat acatccgtca tggtcagtgc ctttcgttag
     1981 ctcgaatgag cactctcgct aggaagtccg tggcccggga tcaccatagt tcgccggcct
     2041 ctccgcggca acacggactg atgcgtggag aagttttgat aggccgtgcc tgggctgcgc
     2101 tgcctgtgtc cgttggtgcg tgatcaacgc gcggaggcct cgagggttgc cagtgcggac
     2161 ggggcgatga ctgcgcgcac acacggacca agttcgaccc gaaacgtccc acgatcccct
     2221 atcaggatga ttacctgttc gcctcacgca agctggccga gcgactcggg gactgcatcg
     2281 cccttcccgt caccggggac tcgcccagtg atcacgcccc gattgtggcg acgttcgata
     2341 tctgaagata gcggtgatgt tcttccgcgc gatcgtgttg cgcacctcga agtagccaag
     2401 ggcacctacg acgatcgagc ccgcccgaac aggatggcgg gccctccgct ttccggaccc
     2461 tgcaagaggc caaagtgtta caggatatgg actccgcctg gatgcgggag gctctgtcag
     2521 cgccggccgg aaggctccgt tggcacacac gcgatgcgga tgtgcacaca tcgcggtggg
     2581 gccgagagat ccaggatacg tcagtcgcat cttgcgggtg actctcgatg agtgctaagc
     2641 cttccatggg atcggacccc atctctcatg ttcaccgaag agggggtgag gcgagccaag
     2701 ggagtccgtc ccttcggtcc gtggttcttc agtctggagg ggatccaatg acccaacgtc
     2761 cggtcagaat ggctcgtctc gcattcttcg ttctcctcat tggcttggtc gcattgcctg
     2821 tcacggctgc tcccaaggac aggaaggtgg gagcggacgc gctcacgcac tcctctcaat
     2881 ccgtggccat tcg
//
LOCUS       DATITW010000060         1968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_563486, whole genome shotgun sequence.
ACCESSION   DATITW010000060 DATITW010000000
VERSION     DATITW010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563486"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VLS86_00755"
     CDS             <1..407
                     /locus_tag="VLS86_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO49047.1"
                     /translation="AWDLIRRIRERGTTVVLVTHFMEEAESLCDRLIVVDDGVVVAEG
                     TPAGLIDRHGGGVRVKFTLDGEDPAWLSSVPHVKELERQHDRVEVRGEGPVLAYVASA
                     LVDRGIAPIDLRQERASLEDVFLNLTSEGSES"
     gene            404..1141
                     /locus_tag="VLS86_00760"
     CDS             404..1141
                     /locus_tag="VLS86_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49048.1"
                     /translation="MSALAKMTWLELKLFLREPLTVLFAVVLPLMILFVMGGVFGNTA
                     DADVYRGVGAMTYYLPAYVALVAASVGLISLPTHLASNRELGVLKRYRASSIPAYVVV
                     GSQVGVTFIISMISALILVAVAVPVYDLPAPSSYPMVVAGFALVALAFSALGVLLGAV
                     LPSSRAAQALGVMLWFLMLMVGGAGPPPEVLTGAMGTVGDFTPLRHAIRAMQDGWLDL
                     DPGISWPVVGGITVLSTAAAVYFFRWE"
     gene            1225..>1968
                     /locus_tag="VLS86_00765"
     CDS             1225..>1968
                     /locus_tag="VLS86_00765"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HSO49049.1"
                     /translation="MATLSRLAAIPVTEVKSLGPKRAAVLAKSGIVTVADLLHHVPRT
                     YLDRSDKPPLARVELGTEVTIIAEVKKIGTRRPKPKLAMTEAVISDDTASLKIVWFNQ
                     VYREKMLPPGTQAAFSGTVESFRGKLQMSNPAVDVLDGGEPLVTGRIVPIYPQVGEVK
                     SGRFLHAVHNALKRARPIPDPMPDVLLAVNGLMARDQAFTSIHFPESVEQSDQARRRL
                     VYDEFFRLELSLALRKRRQIEDATGFAHNP"
BASE COUNT          303 a          632 c          648 g          385 t
ORIGIN      
        1 tggcgtggga cctgatacgc cggattcggg aacggggcac cacggtggtt ctcgtcactc
       61 acttcatgga ggaggcagag tcactctgcg atcgtctcat cgtcgtcgac gacggcgtcg
      121 tggtggccga aggcacgccc gccggtctca tcgaccgtca tggtggggga gtgagggtga
      181 agttcaccct cgacggtgag gatcctgcct ggctgtcgtc agtgccccac gtcaaggagt
      241 tggagagaca acacgacagg gtcgaggtca ggggggaggg gccggtgctg gcctatgtcg
      301 cctcggctct ggtggaccgc ggcatcgccc ccatcgacct ccgtcaagaa cgagcgagtc
      361 tggaagacgt gtttctcaat ctcacctcgg aggggagcga gtcgtgagcg cattggccaa
      421 gatgacctgg ctggagttga agctgtttct gcgggagccg ctcactgtcc tcttcgccgt
      481 ggtgctcccg ctgatgatcc tgttcgtcat gggcggggtg ttcggcaaca cggccgacgc
      541 cgacgtctat cggggggtgg gggcgatgac ctactacctc ccggcgtacg tcgctctggt
      601 ggcggcctcg gtgggtctga tcagtctccc cacccatctg gcgagcaatc gggaactggg
      661 cgtgctcaag cggtatcggg catcctcgat acccgcctac gtcgtcgtcg gctcacaggt
      721 cggggtgacc ttcatcatct cgatgatcag cgccctgatc ctggtcgcgg tcgcggttcc
      781 ggtgtacgac cttcccgccc cgtcttcgta cccgatggtt gtcgccggct ttgccctggt
      841 tgcactggcc ttctccgccc tgggagtcct cctcggcgca gtcctcccgt cgtcgcgggc
      901 ggcgcaggcg ttgggggtga tgctctggtt cctgatgctc atggtgggcg gtgccggccc
      961 accaccggag gtcctgaccg gtgcgatggg aacggttggt gatttcaccc cgctccgcca
     1021 cgcgatccgg gcgatgcagg acgggtggct cgacctcgat cccggcatct cgtggccggt
     1081 cgtcggtggg atcaccgtgc tctccacggc tgccgctgtc tacttcttcc gctgggagta
     1141 gatccgaacc gtccggtcca tctcccattc ctgcaccggt tgtaccgttg acctcgtgcg
     1201 aaatcgcccc cgccgtgagc agtcgtggcc acgctgagcc gtctggcagc gattccggtg
     1261 accgaggtca agtcgctggg gccgaagcga gccgcggtgc tcgccaaatc cgggatcgtc
     1321 acggttgcgg acctgctcca ccacgtgccg aggacctacc tcgaccgatc cgacaagccg
     1381 ccgctggccc gtgtcgagtt ggggaccgag gtgacgatca tcgccgaggt gaagaagatc
     1441 ggcacccgcc gccccaagcc gaagctggcg atgaccgaag ccgtgatctc cgatgacacg
     1501 gcatcactca agatcgtctg gttcaaccag gtctatcggg agaagatgct cccaccaggc
     1561 acccaggctg cgttctcggg gaccgtcgag tccttccggg gaaagctgca gatgtcgaac
     1621 cctgcggttg acgtcctcga tggtggtgag ccgttggtga ccggtcgcat cgtcccgata
     1681 tatccccagg tgggcgaggt gaagtcgggg aggttcctcc acgcggtgca caacgccctg
     1741 aagcgagcca gaccgatccc ggatccgatg cccgatgtcc tcctcgccgt caacggcctg
     1801 atggcccggg atcaggcctt cacgtcgatc catttccccg agtccgtcga gcaaagcgac
     1861 caggcgcggc gacgtctggt gtacgacgag ttcttccggc tcgagctatc gcttgcgctc
     1921 cgcaagcggc gtcagatcga agacgccacg ggctttgccc acaacccg
//
LOCUS       DATITW010000061         1526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_42879, whole genome shotgun sequence.
ACCESSION   DATITW010000061 DATITW010000000
VERSION     DATITW010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42879"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VLS86_00770"
     CDS             complement(<1..1031)
                     /locus_tag="VLS86_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HSO49050.1"
                     /translation="MTGTENTKRRSYLLIAMVMALLITAFGVTSPADATHNSTPKVDV
                     RLTLLHNNDAESALLETLVEGQPFGGVAPFATQVDQLRKQGDRGRDNVSLLVSSGDNY
                     LASAQFQASLDKGVPFYDAMAMDLIGYDAVAIGNHEFDFGPDVFADFVRSFKSRTPFL
                     SSNLDFSNEPVFDRLVRRGRIAASVIVREEGEKFGIIGATTDFLPIISSPRDVIVNEV
                     LPAVQAEVERLKSKGVDKIILISHLQSILEDLDLAAMLNDIDIVVAGGGDELLANDDD
                     LLVPGDLAAVFGSYPITALDIDGTEVPVVTTSGGYRYVGRLIVDFDSRGNVIQVDDES
                     GPVRVTTGE"
BASE COUNT          302 a          479 c          467 g          278 t
ORIGIN      
        1 aactccccgg tggtgacccg gaccggcccg ctctcgtcgt cgacctggat cacgtttcct
       61 cgactgtcga aatcgacgat caaacggccg acgtagcgat agcccccgct ggtggtgacc
      121 accggcacct cagtgccgtc gatatcgagg gcggtgatcg ggtaggaacc gaaaacggct
      181 gccaggtcgc cggggaccag aaggtcgtca tcgttggcca gaagctcgtc cccgcccccg
      241 gcaaccacga tgtcgatgtc gttcagcatc gcggcgaggt cgagatcctc gaggatgctc
      301 tgcagatggg agatgaggat gatcttgtcg acacccttgc tcttcagcct ctcgacttcg
      361 gcctgtactg ctgggagcac ctcattgacg atgacgtctc ggggactgga gatgatgggg
      421 aggaagtccg ttgtggcgcc gatgatcccg aacttctcgc cctcctccct cacgatcacg
      481 ctggctgcga tcctgccccg acggaccaac ctgtcgaaca ccggctcgtt ggagaagtcc
      541 agattggagc tgaggaacgg ggtcctcgac ttgaagctcc tgacgaagtc ggcgaataca
      601 tcggggccga agtcgaactc atggttcccg atggcgaccg cgtcgtagcc gatcaaatcc
      661 atggccatcg cgtcatagaa ggggacgcct ttgtcgagac tcgcctggaa ctgggcgctg
      721 gccagatagt tgtcgccaga ggacacgagc aacgagacgt tgtcccgtcc cctgtcaccc
      781 tgtttccgca actgatcgac ctgagtggcg aacggcgcga cacctccaaa cggctgaccc
      841 tcgaccagcg tttccagcaa ggccgactcc gcgtcgttgt tgtgcagcag cgtgagccgg
      901 acgtcgacct tcggagtcga gttgtgggtg gcgtcggccg ggctggtcac gccaaacgcg
      961 gtgatgagca gggccatcac catggcgatc aggagatacg acctccgctt cgtgttctct
     1021 gttcctgtca tgaaaacccc tttgacttga tccgacccga cgggccggcc ccatggttca
     1081 ccctccaaac aacacgtcgg ttacccgacg gcgaaagtct ggagacgatt gggtcgagaa
     1141 tgcgggaaca cctcctcatc ggttgagagc gaactgctcg cgataggaat cccggaacgg
     1201 gcgtgcccgg ggtcggcaca gatgcgatgt ctggcctgtt gtgccaagtc ggtctacttc
     1261 ccccccgacc cgcgtttcaa accctacaga tccgacaaca agagtgggct gtgccaataa
     1321 gggcaacaag aagggtcttg ctcaggaccg gccgtcgatg ccgataaacc tccgatggct
     1381 gggatcatgg acccgaggga caccgagcac caccacccca tggcagacca agagactttc
     1441 gccgagacgc ttgccgcggc caaggccggc tccgaggcgg cgtgggccac tctgtaccgg
     1501 agactcgccg ggccggtcac cggata
//
LOCUS       DATITW010000062         2646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_403324, whole genome shotgun sequence.
ACCESSION   DATITW010000062 DATITW010000000
VERSION     DATITW010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403324"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VLS86_00775"
     CDS             <1..815
                     /locus_tag="VLS86_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014911941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HSO49051.1"
                     /translation="ASAAFTESWDHFGFQVDDGVATVTFSRPDRLNALTFDIYADLRD
                     LLAELEHRDDVATLVITGEGRGFCSGGDVDEIIGSLVGMDSKGLLEFTRMTGSVVERI
                     RRLPIPVIAAVNGVAAGAGAVIALASDLRILAESASFQFLFTKVGLAGADMGSAYLLP
                     RVVGLGRATEMLLLGEKVEAVDAVAVGLATKVVPDVDLAADVAGLARRLAAGPALAYG
                     ATKVLIGRELDMDLASAIELEAFTQALLMTTKDHAEFYEAFTGKRPPKWKGR"
     gene            812..1213
                     /locus_tag="VLS86_00780"
     CDS             812..1213
                     /locus_tag="VLS86_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017558936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HSO49052.1"
                     /translation="MSRHHLFNPNGMTPPFGFSHGALSAEGRTLYIAGQTGHHPDLDI
                     DDGLVEQFAQACRAVATVIEDAGGAPDDLVSLTIYTTQINSYREHLGPIGAAYRQVFG
                     RHFPPMALIGVSELFDPRAVVELVGVAVVNP"
     gene            complement(1210..2355)
                     /locus_tag="VLS86_00785"
     CDS             complement(1210..2355)
                     /locus_tag="VLS86_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HSO49053.1"
                     /translation="MNLKPGFSRFLDAAPGRLHFAAHSHHPWPDVSFEAHQQMWLDAA
                     RLVDDKWDHIFGDVIPDTQSKVASILGLSDPTTLTFGPNTHGFLARIFSCLEPPVRVL
                     TSDAEFHSFNRQSRRWEEAGLAIVDRVPAEPFETFADRFTREARAGVHDLVYLSQVFF
                     DSGFVVPELDRVVSSVSEDRAYVVIDGYHGFMARPTNLGAIQDRVFFLAGGYKYAMAG
                     EGACFLHSPPGYGARPVDTGWYAGFGQLETGVSGQIPYSQDGSRFAGATYDVSGIYRL
                     QAVLRWLQGSGVTVADIRAYVVDLQDRFLGGASLPGELIPPLPQDRGNFLTFRSDDAG
                     GIYRSLHQRAVITDYRADRLRIGFGIYQDEADVDRLIAVLAELPAAR"
     gene            complement(2352..>2646)
                     /locus_tag="VLS86_00790"
     CDS             complement(2352..>2646)
                     /locus_tag="VLS86_00790"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HSO49054.1"
                     /translation="YRDQPALHEPYQMLESLMDIDEGFTAWRHRHAQMALRMIGRRIG
                     TGGSAGAKYLEKSAERSRIFGDLFDLSTFLIPRDSRPPLPRDLVEAMRFRYQG"
BASE COUNT          474 a          823 c          888 g          461 t
ORIGIN      
        1 gcgcctctgc cgcattcacc gaatcctggg accatttcgg attccaggtc gacgacggcg
       61 tggcgacggt caccttcagt cgtccggatc gcctcaatgc gctcactttc gacatttacg
      121 ccgacctgag ggacctgctc gctgagttgg agcaccgcga cgatgtggcg actctggtca
      181 tcaccggcga agggcgaggg ttctgctccg gcggggatgt cgatgagatc atcgggagcc
      241 tggtcgggat ggactccaag ggtctgctcg agttcacccg gatgactgga tccgtggtgg
      301 agcggatccg gcggcttccg atcccggtga tcgccgctgt caacggagtg gccgcgggcg
      361 cgggggcggt gattgccctt gcttccgacc tgaggatcct ggctgaatcg gcctcctttc
      421 agttcttgtt caccaaggtg ggacttgccg gggccgacat gggctcggca tatctgctcc
      481 ccagagtggt ggggctgggg cgggccaccg agatgctcct gctaggggag aaggtggagg
      541 cggttgatgc tgtcgcggtc gggctcgcca ccaaggtggt tcccgacgtc gatcttgccg
      601 ccgacgtcgc cggcctggct cgtcgtctcg ccgcggggcc ggcactcgcc tacggcgcga
      661 ccaaggttct gatcgggcgt gaattggaca tggacctggc ctcggcgatc gagttggagg
      721 ccttcaccca ggcgttgttg atgaccacca aggaccacgc cgagttctac gaggcgttca
      781 ccgggaagcg accgccgaag tggaaaggcc gatgagccga caccatctct tcaaccccaa
      841 tgggatgaca ccaccattcg ggttctccca tggagcccta tccgcagagg gccgcaccct
      901 ttacatcgcg ggtcagaccg gtcatcaccc cgacctggac atcgacgacg gcttggtcga
      961 gcagttcgcc caggcctgtc gggcggtggc cacggtgatc gaggacgccg gcggagcccc
     1021 agacgacctg gtgtcgctga ccatctacac cacccagatc aactcctatc gggagcacct
     1081 ggggccgatc ggcgccgcct accgccaggt gttcggacgg catttcccgc cgatggccct
     1141 gatcggtgtc tccgagctct tcgaccctcg cgcggtggtg gagttggtcg gggtggcggt
     1201 ggtcaatccc taacgggcgg cgggcagctc ggccaacacc gcgatcagac ggtcgacatc
     1261 ggcctcgtct tgatagatgc cgaagccgat gcgaagccga tcggcccggt agtcggttat
     1321 cacggcgcgt tggtgcagtg agcgatagat cccaccggcg tcatccgatc tgaaggtgag
     1381 gaagttgccg cggtcctggg gcaacggcgg aatcagctcc ccgggaaggc tcgcaccgcc
     1441 gagaaaccgg tcctggagat cgacgacata ggcccggatg tccgcgaccg tcacccccga
     1501 tccctggagc cagcgcaaca cggcctgcag tcgatagatc cccgagacgt cataggtggc
     1561 cccggcaaac ctcgagccgt cctgggagta gggaatctgc ccgctgacac cagtctccag
     1621 ttggccgaag ccggcatacc aaccggtgtc aaccggccgg gccccgtagc caggcgggga
     1681 gtggagaaaa caggccccct cgcctgccat ggcgtacttg taacccccgg ccaggaagaa
     1741 gacccggtcc tggatggcgc cgaggttggt gggtcgcgcc atgaacccgt ggtaaccgtc
     1801 gatgaccaca taggcacgat cctcggatac cgaggagacc acccggtcca gctcgggaac
     1861 gacgaatccg gaatcgaaga agacctggct gagatacacc agatcgtgaa cccctgctcg
     1921 ggcttcacgg gtgaaacgat cggcgaaggt ctcgaacggc tcggcgggaa cccggtcgac
     1981 gatggcgaga cccgcctcct cccatctccg tgactgccgg ttgaatgagt ggaattcggc
     2041 atcgctggtg agcacccgca ccggcggctc gagacaggag aagatccggg ccaggaagcc
     2101 atgggtgttg ggcccgaagg tgagtgtggt ggggtcggag aggccgagga tcgaggcgac
     2161 cttgctctgc gtgtcgggga tcacgtcgcc gaagatgtgg tcccatttgt catccaccag
     2221 ccgagccgca tccagccaca tctgttggtg ggcttcgaac gagacgtccg gccatgggtg
     2281 gtgggaatgt gccgcgaaat ggagacgccc cggagcggca tccaggaagc gagagaagcc
     2341 cggcttcagg ttcatccctg ataccggaat ctcatcgcct ccacgaggtc tcggggaagt
     2401 ggcggccggc tgtcccgggg gatgaggaag gtcgagaggt cgaacagatc gccgaagatc
     2461 ctgctccgct ccgccgattt ctccagatac ttggcgcctg ccgatccacc ggtcccgatg
     2521 cggcgtccga tcattctcag agccatctgg gcatggcgat gacgccacgc ggtgaagcct
     2581 tcgtcgatgt ccatcagcga ctccagcatc tgatacggct cgtggagggc gggctggtcc
     2641 cgatag
//
LOCUS       DATITW010000063         3610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_243220, whole genome shotgun sequence.
ACCESSION   DATITW010000063 DATITW010000000
VERSION     DATITW010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243220"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VLS86_00795"
     CDS             complement(<1..669)
                     /locus_tag="VLS86_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSO49055.1"
                     /translation="MPNDRLSSALSVEVTALREAGTDKGIETVITGVEPAQGGLGPRL
                     RLAGSDERYLRMNSNSYLGLSRHPTVIDADEKATKEFGAGPGAVRFISGTYQAHIDLE
                     GTLARFHGRAAAMAFSSAYATVLSTLTTLITPETVVLSDELNHNCIINALRLARPGAR
                     EVYPHLDYSVLDAKLSEARGAGRRVIVVTDGVFSMRGDFADLAALMDLARAHDGHFEE
                     NVVVV"
     gene            708..1589
                     /locus_tag="VLS86_00800"
     CDS             708..1589
                     /locus_tag="VLS86_00800"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSO49056.1"
                     /translation="MTRLGFIGLGIMGHGMAVNLLASGNPLTVWNRTPEKAVDLVCDV
                     ALNPHDLGEASDIVFVCVSDTPDVEEVVFGDFGVIHGMTEGDILVDHSTISPSATKDF
                     SMRAAELGVTWLDAPVSGGSEGAEQGTLAVMVGGPPATLETVRPFMEAYSKSIVHVGE
                     EPGSGQMAKAVNQVLVVLNQLAASEALLLAQGAGLDLTMTLEAVEGGAAGSWMLSNRG
                     PQMIRRDWRPGFTIDLQQKDLRLVLEAADELGVPMPGVSLVFQLYRALQAQGLGSEGN
                     HALVKALEALAGIRVGD"
     gene            complement(1607..2053)
                     /gene="bcp"
                     /locus_tag="VLS86_00805"
     CDS             complement(1607..2053)
                     /gene="bcp"
                     /locus_tag="VLS86_00805"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HSO49057.1"
                     /translation="MSETVSFNLPNQDGKLVSSQDFAGRKLVMFFYPAAMTPGCTKES
                     CDFRDSYEELTAAGYEIVGVSPDSPARNAKFKEKEGLNFDLLSDEDTQLATTLGAWGE
                     KTLYGKLMEGLIRSTFVIGPDGSVEKAYRNVKATGHVARVKEDLLG"
     gene            complement(2086..3021)
                     /locus_tag="VLS86_00810"
     CDS             complement(2086..3021)
                     /locus_tag="VLS86_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HSO49058.1"
                     /translation="MSPKRVAWIAVIAAVLYVGVAGFVLNNFELPEMDSLAWTQVGLA
                     ALAQITAAWFFGLLYKESVEGAGGTLKAFSAFKAALVGAGVARLIPAGGAITPVAMAW
                     TVRDEEPGTTGAAVRTVLLNYAGLLMMTGVGLLLARPRESAQLFSISLAVLAPFVLFA
                     GLVLMFGSGKLGSLSKYLPKVLRKRLDATVIDNLPGPESQTYIWCRLALEGAALGLVL
                     RAFGIEVNVFQVMAAFGVAQLAGGLPGTPGGLGVTEAGLVFILSAYGFPSRETLAPVI
                     IYRIVSYWLPAALSFFAGGMTFLRSPEAKAVSEAD"
     gene            complement(3018..>3610)
                     /locus_tag="VLS86_00815"
     CDS             complement(3018..>3610)
                     /locus_tag="VLS86_00815"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HSO49059.1"
                     /translation="FAQVIGRVRGVDLRQVGRGNVGAGNLTRVAGWPWGLAAGVCDGL
                     KGLFPVLIVQNAGFGKGAAGMVGLMAVIGHNWSLFMRGRSGRGLATAAGMLAALDPVL
                     LVWTTGWSVAGWKIGGGVAGFLGWGLLPIVAATMGRPPTEWFVLLVLSGVLMARRMQG
                     NPESPRGVRPAMRRAVFDTDPGGEDFPRTADDPLTP"
BASE COUNT          671 a         1193 c         1179 g          567 t
ORIGIN      
        1 caccaccacc acgttctcct cgaaatgccc gtcgtgggcc cgagccaggt ccatgagcgc
       61 ggcgagatcg gcgaagtcgc cccgcatcga gaacacgccg tcggtgacga cgatcacccg
      121 cctcccggcg cccctcgcct cgctcaactt ggcgtcgagc accgaatagt cgagatgggg
      181 gtacacctcc ctggcccccg gccgcgccag ccgtagggcg ttgatgatgc aattgtggtt
      241 gagctcgtcg ctcagcacca ccgtttccgg cgtgatcagc gtggtcaggg tggacagcac
      301 ggtggcgtag gccgaggaga acgccatcgc cgcggctcgc ccgtggaatc gggcgagagt
      361 cccttcgaga tcgatgtgag cctgataggt gcccgagatg aagcgcaccg ccccgggacc
      421 ggcgccaaac tccttggtcg ccttctcgtc ggcatcgatc acggtcgggt gtcgggacag
      481 gccgagatag ctgttggagt tcatcctcag gtagcgctca tccgatccgg cgagccgcag
      541 tcggggaccg agaccaccct gagcaggctc cacccccgtg atgacggtct cgatcccttt
      601 gtcggtcccg gcttcgcgga gggcggtgac ctcgaccgag agtgcagagc tgagacgatc
      661 gttgggcatg cagcccgggt ttagcacacc tatagagtgc gccggcaatg actcgactcg
      721 gattcatcgg actggggatc atgggccacg ggatggcggt caatctgctc gcatccggaa
      781 acccgctcac ggtgtggaat cggaccccgg agaaggcggt ggacctcgtt tgcgacgttg
      841 ccctcaaccc gcacgacttg ggcgaagcca gtgacatcgt cttcgtctgc gtctcggaca
      901 ctcccgatgt ggaggaagtg gtctttggcg actttggcgt gattcacggg atgaccgaag
      961 gcgacatcct cgtcgaccac tcgacgatct cgccaagcgc caccaaggac ttctcgatgc
     1021 gtgccgccga gctcggtgtg acctggctcg atgccccggt gtccggtgga tccgagggcg
     1081 cggagcaagg gacgctggcg gtgatggtgg gagggccacc ggcgactctc gagacagttc
     1141 gcccgttcat ggaggcctac tcgaagtcga tcgtgcatgt cggcgaggaa cccggctcgg
     1201 gtcagatggc caaagccgtc aatcaggttc tggtggtact caatcagttg gctgcgtccg
     1261 aggctctgct cctcgcccaa ggcgcaggtc tcgacctgac gatgacgctc gaggcggtgg
     1321 agggcggggc ggccgggtcg tggatgttgt ccaaccgcgg gccacagatg atccggcgtg
     1381 actggaggcc gggcttcacc atcgacctcc agcaaaaaga cctgcggctg gtgctcgagg
     1441 cggccgacga gctgggtgtc cccatgcccg gggtctcgct cgtcttccag ctgtaccggg
     1501 ccttacaggc acagggtttg gggtcggagg gcaatcacgc tctggtcaag gcactcgagg
     1561 cgttggccgg gatccgggtc ggggactgag aaccaccgaa aaggcgctag ccgagcaggt
     1621 cttccttgac ccgggccacg tgaccggtgg ccttgacatt gcggtatgcc ttttccaccg
     1681 agccgtcggg cccgatgacg aaggtcgagc gaatcaggcc ttccatcagc ttgccgtaga
     1741 gcgtcttctc cccccaggcc ccgagcgtgg tcgccagttg cgtgtcctcg tcggagagca
     1801 agtcgaagtt gagcccctcc ttctccttga acttcgcgtt gcgggccgga gaatcggggc
     1861 ttaccccgac gatctcgtac ccggcggcgg ttaactcctc gtagctgtcc cggaaatcgc
     1921 acgattcctt ggtgcacccc ggggtcattg ccgccgggta gaagaacatc accagcttgc
     1981 ggccggcgaa gtcctgggac gaaacgagtt ttccgtcctg attggggagg ttgaagctca
     2041 cggtctcgga catgggacca gggtagtgag cgagccggcg accctctagt cggcctccga
     2101 gaccgccttg gcctccgggg acctgaggaa cgtcatgcca ccggcgaaga agctgagggc
     2161 agcggggagc cagtaggaca cgatccggta gatgatcacc ggggccagcg tctcccggga
     2221 cgggaacccg taggcggaga ggatgaagac gagcccggcc tcggtgactc ccagaccacc
     2281 cggcgttccc ggcaggccac cggcgagctg ggccacgcca aacgcggcca tcacctgaaa
     2341 cacattgacc tcgatgccga aagccctgag aaccagaccg agggcggcgc cctcgagagc
     2401 gagacggcac cagatgtagg tctgcgactc cggccccggg agattgtcga tcaccgtggc
     2461 atcgagacgc ttccgaagga ccttgggcag gtacttggac agcgaaccga gctttcccga
     2521 gccgaacatc agcactaggc cggcaaagag cacgaagggg gcgagaacag caaggctgat
     2581 cgagaacaac tgagccgact cccggggtcg ggccaggagg agacccacac cggtcatcat
     2641 caacagaccg gcgtagttga gcaagactgt ccgcaccgcg gcgccggtgg tccccggctc
     2701 ctcatcacga accgtccagg ccatcgccac cggggttatc gccccgcctg ccgggatcaa
     2761 ccgggcaacc ccggcgccga ccagtgcggc cttgaacgca gagaatgctt tcagggtccc
     2821 cccggcgccc tccaccgatt ccttgtagag gagcccgaag aaccatgccg ccgtgatctg
     2881 ggccaacgcc gccagcccga cctgggtcca ggcgagcgag tccatctccg gcaactcgaa
     2941 gttgttgaga acgaagcctg cgactccgac atagagcacc gccgcgatca cggcgatcca
     3001 tgcgacccgc ttcgggctca cggggtcagc ggatcgtcag cagtccgggg aaagtcttcg
     3061 ccgccagggt cggtgtcgaa gactgcacgc ctcatggccg gtcgaactcc acgcgggctt
     3121 tcgggattgc cctgcatgcg tctcgccatg agcactccgg agagcacgag caggacaaac
     3181 cactcggtgg gaggacggcc catggtggcc gcgacgatcg gcagcagccc ccagcccagg
     3241 aacccggcga cgccgccgcc tatcttccac ccggcgaccg accaacccgt cgtccaaacc
     3301 aggagcaccg gatcgagggc ggcgagcatc ccggccgcgg tggccagacc gcgccccgaa
     3361 cgaccacgca tgaacagaga ccagttgtga ccgatcacag ccatcaaccc gaccatgccc
     3421 gcggcgccct tgccgaaccc ggcgttctgg acgatgagga cagggaacag acccttgagc
     3481 ccgtcgcaca ctccggcggc gaggccccag ggccaacccg ccaccctggt cagattgccg
     3541 gcgcccacat tgccacggcc cacctggcga aggtcgactc cccggacgcg accgatcacc
     3601 tgggcgaacg
//
LOCUS       DATITW010000064         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_764112, whole genome shotgun sequence.
ACCESSION   DATITW010000064 DATITW010000000
VERSION     DATITW010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764112"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /gene="rplT"
                     /locus_tag="VLS86_00820"
     CDS             complement(<1..259)
                     /gene="rplT"
                     /locus_tag="VLS86_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HSO49060.1"
                     /translation="MARVKRAVHAKKSHKKVLARAKGYKGARSRRYRTANEQVMHAMQ
                     DAYRDRRARKGEFRRLWIARINAAARQNGTTYSQLIAGLKAA"
     gene            complement(278..472)
                     /gene="rpmI"
                     /locus_tag="VLS86_00825"
     CDS             complement(278..472)
                     /gene="rpmI"
                     /locus_tag="VLS86_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HSO49061.1"
                     /translation="MPKMKTHSGAKKRIKVTGSGKYMRRQSGRGHLRLAKGKNVFRRR
                     SGEVELAPGDAKVAKKLLGR"
     gene            complement(491..1060)
                     /gene="infC"
                     /locus_tag="VLS86_00830"
     CDS             complement(491..1060)
                     /gene="infC"
                     /locus_tag="VLS86_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HSO49062.1"
                     /translation="MNGAIRAKEVLVIAPDGAQIGVKKLNEALWLAEQLDLDLVEVAP
                     NAKPPVCRLMDYGKYKYEQSVKAREARKKQTRTTIKELRMKVRIGEHDFDITRRKAQE
                     FLADGDKVKVTMRFRGREQERPSFGKELLDRLAEDLDGFGVVEQNPKLEGRSMTMVIA
                     PARRPRAVVEHAEDVVEISEQVVEISEEE"
     gene            complement(1300..1764)
                     /gene="ribH"
                     /locus_tag="VLS86_00835"
     CDS             complement(1300..1764)
                     /gene="ribH"
                     /locus_tag="VLS86_00835"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HSO49063.1"
                     /translation="MTRFVEGDRDATGLHVGIAVASWNRAVTDRLLEGALGRLGELGA
                     DPVTVLRVPGALELPLGARRLVDRGCDAVVAIGTVVRGDTDHYDIVVRESTAGVARVS
                     LDTGVPVANAILAVNEYAQAIERAGEGPANKGHEAAEAAVLMAVALRRLGEG"
     gene            complement(1761..>2138)
                     /locus_tag="VLS86_00840"
     CDS             complement(1761..>2138)
                     /locus_tag="VLS86_00840"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894823.1"
                     /note="bifunctional enzyme DHBP synthase/GTP
                     cyclohydrolase II; functions in riboflavin synthesis;
                     converts GTP to
                     2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine;
                     converts ribulose 5-phopshate to 3,4-dihydroxy-2-butanone
                     4-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HSO49064.1"
                     /translation="RVGAEGRGVVLYIRGHEGRGIGLLHKLAAYKLQDEGFDTVDANV
                     NLGLPVDARDYGVGAQILYDLGVRSMRLMTNNPTKRAGIEGYGLSIIEQVPLSVDAND
                     ENRAYLRAKATRLGHQMDLGQGA"
BASE COUNT          318 a          744 c          642 g          434 t
ORIGIN      
        1 cggccgcttt cagccctgcg atgagttggg agtacgtggt cccgttctga cgcgcggcgg
       61 cgttgatccg ggcaatccac agccgtctga actcgccctt gcgggcgcgc cggtcgcgat
      121 acgcatcctg catcgcatgc atgacctgct cgttggcggt ccgatagcgt cggctgcggg
      181 cacccttgta ccccttggct cgggccagca ccttcttatg tgacttcttg gcgtgaaccg
      241 cgcgcttgac acgtgccatt tcgttcctcc tgagctatca gcgaccgagc agcttcttgg
      301 cgactttggc gtcgcccggt gccagctcga cttccccgga cctgcgccga aagacgttct
      361 tgcccttggc cagacgcaaa tgcccgcggc cggattggcg tctcatgtac ttgccggacc
      421 ctgtgacctt gatccgcttc ttggcgccac tgtgcgtctt catcttgggc acggcatcct
      481 cctgttcgtc ctactcctct tcggagatct cgaccacctg ttcggagatc tcaaccacgt
      541 cttcggcgtg ctccaccacg gccctcggcc tcctggccgg agcgatcacc atcgtcatgc
      601 tccgcccttc gagctttggg ttctgctcga ccacgccaaa gccgtccagg tcctcggcca
      661 gccggtcgag gagctctttg ccaaagctcg ggcgctcctg ctcccggccc cggaaccgca
      721 tcgtgacctt caccttgtcc ccgtccgcca ggaactcctg tgccttgcgg cgggtgatgt
      781 cgaagtcgtg ctccccgatc cgcaccttca tccggagctc cttgatcgtg gtacgggtct
      841 gcttcttgcg agcctcgcgg gccttcaccg attgctcgta cttgtacttc ccgtaatcca
      901 tcaggcggca caccggcggc tttgcgttgg gtgccacctc gacgagatcc agatcgagtt
      961 gctctgcgag ccatagggcc tcgttcaact tcttcacccc gatctgtgcg ccgtcaggtg
     1021 caatgacaag cacctctttg gccctgatcg cgccgttcac acggagctcg ttgatgctga
     1081 tacctcctgt tgattgcagc cactactgag catgaaaaat ggcagcaggg tgaaatctgc
     1141 tgccataggc agggtcactg gacccgttgg accacggctc gccattggaa tcaagaagat
     1201 ctcacagggc ggccgggtgg ggaagcagca gcttccgctc ttgctatcaa ttgtgcccgg
     1261 gaggttacca gtggggccct cgaaaccggt agtcggatct cagccctcac ccagtcggcg
     1321 caaggccacc gccatgagaa ccgcggcctc ggcggcctcg tgacccttgt tggccggccc
     1381 ctccccggcg cgctcgatgg cctgggcgta ctcgttgacc gcgagtatgg cgttggccac
     1441 cgggaccccg gtgtcgagac tgacccgggc cacgccggcg gtggactccc tgacgacgat
     1501 gtcgtagtga tcggtgtcgc cgcgcaccac ggttccgatg gcaacgacgg cgtcacatcc
     1561 tcggtcgacc aggcggcgcg ccccgagcgg gagctcgagt gcccccggga ccctgagcac
     1621 agtcaccggg tcggcgccca actcgccgag ccgaccgaga gcgccttcga gaagccggtc
     1681 ggttaccgct cgattccagc tggcgacggc gatcccgacg tgcaagccgg tggcgtcccg
     1741 gtctccttcg acgaatctgg tcatgccccc tggcccagat ccatctgatg cccgagacgg
     1801 gtcgccttgg ccctgaggta ggcccggttc tcgtcgttgg cgtcgacgga gagcgggacc
     1861 tgctcgatga tcgacagccc gtagccctcg atcccggccc gcttggtcgg gttgttggtc
     1921 atcagccgca tcgagcggac cccgaggtcg tacaggatct gggcccccac cccgtagtca
     1981 cgagcgtcga ccggaaggcc gagattgacg ttggcgtcca ccgtgtcgaa accctcgtcc
     2041 tggagcttgt atgcggccag cttgtggaga aggccgatcc cacggccctc gtggcccctg
     2101 atgtagagca ccacgccgcg gccctcggcg cccactct
//
LOCUS       DATITW010000065         1840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_443782, whole genome shotgun sequence.
ACCESSION   DATITW010000065 DATITW010000000
VERSION     DATITW010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443782"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1783
                     /gene="nuoL"
                     /locus_tag="VLS86_00845"
     CDS             <1..1783
                     /gene="nuoL"
                     /locus_tag="VLS86_00845"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSO49065.1"
                     /translation="TFEIARIGSDLVFELGWVVDGLSIMMFFLVNVVGLLVFVYAVGY
                     MHGDVRVPWFFAAFSLFAGSMLLLIGAPNLIQLIIGWEGVGLASYLLIGFYWEDLDNV
                     RAGNKAFLVNKVADVGVLLGAIIVALAVGSFDFQAINIAAAEHLEELSSVAVVGGALL
                     FLGAMGKSAQFPFHIWLPDAMAGPTPVSALMHAATMVTAGIYLMARTFPLFQNLAQEA
                     RPWMVAIGAVTLFALGLLALVADDIKKVLAYSTVSQLGYMMTAVAAGGYTAGVFHLFT
                     HAFFKALLFLGAGSVIHAVHSNNMSDMGGLRKDMPITYWTFLVGSLSLAGIIPLAGFW
                     SKDEILATLGHEGYTAVMWIAIAGAFVTAFYMTRAVALTFHGSYKGHGHPHESPRVMT
                     LPLILLAIPSAIAGLVNIPGVSLPGIGNFTEWLAVREVAMGDHHAEAIEWALAGSGIF
                     AALLGIVLGWMIFGRDRETQQQRDAFVVPVLYPLLRNKYYMDDLADGVVGATMGPVAR
                     FVNWTNTYIIDAVVNSVALLTKGLGSFVYGILDQRGVDGAVHGLSAAADGAGSALRKM
                     QTGRVQQYAASFVGGVLALIIVFVFVT"
BASE COUNT          276 a          573 c          586 g          405 t
ORIGIN      
        1 cactttcgag atcgcacgga tcggcagcga cctggtcttc gagttggggt gggtggtcga
       61 cggcctttcg atcatgatgt tcttcctggt caacgtggtc ggcctgttgg tcttcgtcta
      121 cgcagtcggc tacatgcacg gtgacgtcag ggtgccctgg ttcttcgccg ccttttccct
      181 gttcgccggg tcgatgctgc tcctgattgg ggcgccgaac ctcatccagc tcatcatcgg
      241 ctgggaagga gtgggtctcg cctcatacct gctgatcggg ttctactggg aggacctcga
      301 caacgtgagg gcgggcaaca aggcgtttct cgtcaacaag gtggccgatg tcggggtgtt
      361 gctgggagcg atcatcgtcg ccctcgccgt tggatcgttc gacttccagg cgatcaacat
      421 cgccgccgcc gagcacctgg aggagctgtc ctcggtggcg gtggtcggag gtgcgctcct
      481 cttcctgggg gcgatgggca agagcgccca gttcccattt cacatctggc tcccggatgc
      541 gatggccggc cccaccccgg tttcggcatt aatgcatgcc gcgaccatgg tcaccgccgg
      601 gatctatctg atggctcgca ccttccccct gttccagaac ctggcgcaag aggcgcggcc
      661 ctggatggtg gcgatcggcg ccgtcaccct cttcgccctg ggcctgctgg cgctggtggc
      721 cgacgacatc aaaaaggtgc tcgcctactc cacggtttcc caactcgggt acatgatgac
      781 cgcggtggcg gccggcggtt acaccgctgg ggtgtttcac ctattcaccc acgccttctt
      841 caaggccttg ctcttccttg gcgccggctc ggtgattcac gccgtccact ccaacaacat
      901 gagcgacatg ggggggctgc gcaaggatat gccaatcacc tattggactt ttctggtggg
      961 ctcgttgtcc ctggccggca ttatccccct ggccgggttc tggtccaagg acgagatcct
     1021 ggccaccctg gggcacgagg gctacaccgc ggtgatgtgg atcgccatcg ccggggcttt
     1081 cgtcaccgcg ttctacatga ccagggctgt ggctctcacc ttccatggga gctacaaggg
     1141 ccatggccat cctcatgagt cgccgcgagt gatgacgttg cccctcatcc ttttggccat
     1201 cccctcggcc attgccgggc tggtgaacat ccccggggtc tctctgcccg ggatcggcaa
     1261 cttcaccgaa tggctggcgg tgcgtgaggt tgcgatgggc gaccaccacg ctgaagccat
     1321 cgagtgggcg ctggccggga gcggcatctt cgccgcgttg ctcgggatcg tcctgggctg
     1381 gatgatcttt ggcagggacc gcgagaccca gcaacagcgg gacgccttcg tggtcccggt
     1441 tctctacccg ctgctccgca acaagtacta catggacgat ctggcggatg gcgtggtggg
     1501 agccaccatg ggaccggtcg ctcgcttcgt gaactggacc aacacctaca tcatcgatgc
     1561 cgtggtgaac tcggtcgccc tcctcaccaa gggcctgggt tccttcgtct acgggatcct
     1621 cgaccagcgc ggagtcgacg gtgcggttca cggtctttcg gcggccgcgg acggcgctgg
     1681 ttcggccctg cggaagatgc aaaccggccg ggttcagcag tacgcggctt cctttgttgg
     1741 cggggtcctg gccctgatca tcgtgttcgt gttcgtgaca taggagaggc tgagaagaat
     1801 ggaattgttc gaaacctggg gactcaccct catcgtgttc
//
LOCUS       DATITW010000066         1915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_604074, whole genome shotgun sequence.
ACCESSION   DATITW010000066 DATITW010000000
VERSION     DATITW010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604074"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VLS86_00850"
     CDS             <1..195
                     /locus_tag="VLS86_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015497796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HSO49066.1"
                     /translation="DVFTPSAEVVEYQKKVLAAFEEAEARGSASIAVDGKMVDYAVAR
                     VARAIIARADAAARGNREGS"
     gene            192..1292
                     /locus_tag="VLS86_00855"
     CDS             192..1292
                     /locus_tag="VLS86_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSO49067.1"
                     /translation="MTRSRYIADGVGVAVVGSGSIGSLRAEISHRHPSVNFLAVCDIV
                     EDKAKRLADSCQADTWSTEATEIVTRDDVDAVIIASTEDSHFEPAMAAVQAGKAVLVE
                     KPFTILAEEGHKLMAAAEEYGVSIHTGFTQRFRRRYLAIKEHVLKGYVGDVTSAKATI
                     YLTQAVARSVISRAGSTTPSVNTMTYLFDLLAWYLGGTLPATAYAAGSNKGRIHEEFG
                     ALDSTWCVLTYEDGMVANLGVSWELPEFWPAYVASMDFELFGREGVISVKDDHRDVLM
                     ASHKAVPSPYTPDTSMNVAMLGSYMPGDWALGEHFGAMQEETHAFINSVGTGRPDPIL
                     ATGNDGMDVLLVSRAVDESARTGQVVPMTWAR"
     gene            1300..>1915
                     /locus_tag="VLS86_00860"
     CDS             1300..>1915
                     /locus_tag="VLS86_00860"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSO49068.1"
                     /translation="MRDGMLAGITILELGQVIAGNFGGVILADLGAEVIKIEPPSGDT
                     ARNATIAPLHGESAIHLFMNRGKKSVVIDLKDPRGLEMFYSLVSQADVVIDNFRPGVM
                     TRLGIDHETLKRHNPRIITVSVTGFGEYGPAKDKAAFDLVVQASSGHVDITGPADGDP
                     ARVGIPIADIGGGIYSCISVLAALAGRELHGNGQHADVAMLDTLV"
BASE COUNT          332 a          618 c          639 g          326 t
ORIGIN      
        1 gacgtgttca ccccttcggc cgaggtcgtc gagtatcaga agaaggtgct ggccgccttc
       61 gaggaggccg aggcccgggg ctcggcctcg atcgcggtgg acgggaagat ggtggactat
      121 gcagtggccc gggtggcgag ggcgatcatc gcccgtgccg acgccgcggc ccgtggcaac
      181 agggagggat cttgaccaga agcaggtaca tcgcagacgg ggtgggggtt gccgtcgtcg
      241 gcagcgggtc gatcggaagc ctgcgggccg agatctccca tcgtcatcct tccgtgaact
      301 tcctcgcggt ctgtgacatc gtcgaggaca aggcgaagcg tctcgccgat tcgtgccaag
      361 ccgatacttg gtcgaccgag gccaccgaga tcgtgacccg ggacgacgtc gacgcggtga
      421 tcatcgcctc caccgaggac tcccacttcg aaccggcgat ggccgcggtt caggccggaa
      481 aggcggtgct ggtcgagaag ccgttcacca tcctcgccga agaggggcac aagctcatgg
      541 ccgccgccga ggagtacggc gtctcgatcc acaccggctt cacccagcgt tttcgtcgtc
      601 gctacctggc gatcaaggag cacgtgctca agggctacgt gggcgatgtc acctcggcca
      661 aggcgaccat ctacctgacc caggccgtgg cgcgatcggt catatcccgg gcgggctcca
      721 ccaccccctc ggtgaacacg atgacctatc tcttcgacct cctcgcctgg tatctgggcg
      781 ggacgcttcc ggccaccgcc tacgcggcgg gctccaacaa gggccgaatc cacgaggagt
      841 tcggcgcatt ggactcgaca tggtgtgttc tcacctacga agacgggatg gtggcgaatc
      901 tcggggtgag ctgggagctg cccgagttct ggccggcgta tgttgccagc atggacttcg
      961 agctgttcgg gcgggagggg gtcatctcgg tgaaagacga ccatcgcgac gtgctcatgg
     1021 cctcgcacaa ggcagtgccc tctccgtaca ccccggacac ctcgatgaac gtggccatgc
     1081 tcggctccta catgcccggc gactgggccc tgggggagca cttcggcgcc atgcaggagg
     1141 agactcacgc cttcatcaac agtgtcggca ccgggcggcc cgatccgatc ctggctaccg
     1201 ggaacgacgg gatggacgtg cttctcgtct cccgggccgt ggacgagtcg gcccgcaccg
     1261 gccaggtggt cccaatgacc tgggccaggt gaagaacgag tgagggatgg gatgctcgcc
     1321 gggatcacca tcctcgagtt gggccaggtg atcgccggca atttcggtgg ggtgatcctg
     1381 gccgaccttg gagccgaggt gatcaagatc gagcctccgt cgggggacac cgcgcgcaac
     1441 gcaaccatcg ccccgctcca cggggagagc gcgatccacc tcttcatgaa tcgcgggaag
     1501 aagagtgtcg tcatcgacct caaggacccc cgtggcctcg agatgttcta ctccctggtt
     1561 tcccaggccg acgtggtcat cgacaacttc cgccccgggg tcatgacccg tctcgggatc
     1621 gaccacgaga cgctgaagcg gcacaacccg aggatcatca ccgtctcggt gaccggcttt
     1681 ggtgagtacg gcccggccaa ggacaaggcg gctttcgacc tggtggtcca ggcctccagc
     1741 ggtcatgtcg atatcacggg ccctgcggac ggcgacccgg cccgggtcgg catccccatc
     1801 gccgacatcg ggggcgggat ctattcctgc atctcggtcc tggccgccct cgccggtaga
     1861 gagctccacg gcaatggcca gcatgccgac gtggcgatgc tggacaccct ggtct
//
LOCUS       DATITW010000067         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_83410, whole genome shotgun sequence.
ACCESSION   DATITW010000067 DATITW010000000
VERSION     DATITW010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83410"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VLS86_00865"
     CDS             <1..647
                     /locus_tag="VLS86_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49069.1"
                     /translation="TSAGETTSAPGTIATTTTTTTTTTTTTTTTTIDRAASTTIAAAT
                     TTAPVDPGLVVSESLAASSANYRFTSVVLVGEQTLTTITGAVDGSSVAADVVTGSGEV
                     SYVRTPDGEWVTGPDGEWVELEGEPPVAPPLGALVDAGNLVLESGDGTRGVFTGTLGP
                     AAGEAQGLGFSLTVESGLVTEVRYQVDTGGEMAQVITTFTDFGQAGTVTAPDGA"
     gene            complement(675..1961)
                     /locus_tag="VLS86_00870"
     CDS             complement(675..1961)
                     /locus_tag="VLS86_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010504568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HSO49070.1"
                     /translation="MSDLTYDRIRFLWPDHLGLARGKYLSPARAAEGSNHCVALFSLQ
                     LNRVMIPHPGAHHESFPDIEARFSTEDIRPGWEKGTGVVVASITKDGIPVELAPRSIL
                     ERVIGEWKEIGLTPMVGFELEAFLFEPAENRGWQPIHTPGSYVYGTGSAVDPSGTIDE
                     LMEKAHDIGIELESVNSEYDDGQFELTLRYGEALRAADDAFLFRVLAKEIPARRGYLL
                     TFMGKPINGRGGSGIHVNFSLLDENGDNAFVDDSAANGLSELAGRCIAGIVSDHVAMT
                     AVVAPTVNAYKRLQPGSLNGYFANWGLDHRGVAVRVPPSRGPGTRLEHRLADGAASVH
                     LAAAATLVAALRGVKMELECPPAETQDCITGADTDVTVPLSLGAALDALEASKDFVEA
                     FSPGYVEGFLAVKRAEWERYVTHTTDWELGEYLEYY"
     gene            2073..>2501
                     /locus_tag="VLS86_00875"
     CDS             2073..>2501
                     /locus_tag="VLS86_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HSO49071.1"
                     /translation="MRALVIEHDPLSTPDRVGAHLEARGVRLEPFVVVDDIRHPDVTV
                     SFPDDGHDLVVLMGAPWSVYDSNVQGWVRPELEMVRGHLARGTPILGICFGAQSLSAA
                     LGGTVSHSKRPEYGWVSIDSKVEEIAAGPWFQFHHDEFTLP"
BASE COUNT          409 a          846 c          832 g          414 t
ORIGIN      
        1 ccacgagcgc cggggagacc accagcgccc ccggcaccat cgccaccacc accaccacca
       61 ccaccaccac caccaccacc accaccacca ccaccatcga tcgggctgct tcgaccacca
      121 tcgccgccgc cacgaccacc gctccggtcg accctggtct cgtggtgagc gagagcctgg
      181 cggcgagcag cgccaactac cggttcacct cggtggtgct ggtgggggag cagacactga
      241 ccaccatcac cggcgccgtt gacgggagct cggtcgcggc cgatgtggtc accggctccg
      301 gagaggtctc ctacgtgcgg acgccggacg gggagtgggt caccgggcct gacggcgaat
      361 gggtcgagct cgagggggag cctcccgtgg ccccgcctct cggcgccctg gtcgatgccg
      421 gcaacctggt gctcgaatcg ggcgacggga cccggggcgt cttcaccggg accctcggcc
      481 ctgctgctgg tgaggcccag ggtctcggct tctcgctcac cgtcgagagt ggcctggtga
      541 ccgaggttcg ctatcaggtc gacacaggtg gtgagatggc ccaggtgata accactttca
      601 ccgattttgg ccaggcgggc acggtcaccg ctcccgacgg cgcctgagca cagtcctctc
      661 cagggtcagg gctctcagta gtactcgagg tactcgccca gctcccagtc ggtggtgtgg
      721 gtgacgtatc gctcccactc ggcgcgtttg accgccagga acccctccac gtagccaggg
      781 gagaaggcct cgacgaagtc cttggaggct tcgagcgcat ccagtgccgc tccgaggctg
      841 aggggcacgg tgacgtcggt gtccgccccg gtgatgcagt cctgggtctc ggcaggaggg
      901 cactcgagct ccatcttcac cccgcgcagc gcggcgacca gagtggcggc ggcggcgagg
      961 tggaccgagg ccgccccatc ggcgagccga tgctcgagac gggtgcccgg gccgcggctg
     1021 ggggggaccc ggactgcgac cccgcgatgg tcgagacccc aattggcgaa gtacccgttg
     1081 agcgagccgg gctggagccg cttgtaggca ttgaccgtgg gggcgaccac cgcagtcata
     1141 gccacgtggt cactgacgat tcccgcgatg caacgcccgg caagctcgct gagcccgttg
     1201 gcagcggaat cgtcgacgaa cgcgttgtcg ccgttctcgt cgagaagaga gaaattgacg
     1261 tggatgccgc tgccgcctcg cccattgatc ggcttgccca tgaaggtcag caggtagccg
     1321 cggcgggccg ggatctcctt ggccagtacc cggaacagga aggcgtcatc ggcggcgcgc
     1381 agcgcctctc cgtaacggag ggtgagctcg aactgcccat cgtcgtactc ggagttgacc
     1441 gattcgagct cgatcccgat gtcgtgtgcc ttctccatca gctcgtcgat ggtgccgctc
     1501 ggatcgaccg cagacccggt gccgtagaca tacgagccgg gggtgtggat cggctgccat
     1561 ccccggttct ccgccggctc gaaaaggaag gcctcgagct cgaagccgac catcggggtg
     1621 agaccgatct ccttccactc tccgatgacg cgttccagga tcgacctggg ggcgagctcc
     1681 acaggaatgc cgtccttggt gatcgaagcc acgaccaccc cggttccctt ctcccacccc
     1741 ggtctgatgt cttctgtgga gaaccgggcc tcgatatcgg gaaagctctc gtgatgggcc
     1801 ccggggtgcg ggatcatcac ccggttcaac tggagggaga acagagccac gcagtggttc
     1861 gatccctcgg ccgcccttgc cggcgacagg tacttgccgc gggctagtcc gaggtgatcg
     1921 ggccacagga accggatgcg gtcgtaggtc aggtcgctca accacaatct cctctcgcct
     1981 cggtcggccg ggtggacccc aagacagtat caaggggccc cctggctgtg acgacccggc
     2041 gccgtcgcgg gggccattga taagctgccc cggtgcgggc actcgtcatc gaacacgacc
     2101 cactttcgac tccggatcgg gtcggtgccc atctcgaagc gcgaggtgtc aggctcgagc
     2161 catttgtggt ggtcgacgac atccgccatc ccgacgtcac tgtcagcttc cccgacgacg
     2221 gccacgacct ggtggtcctc atgggagctc catggtcggt ctacgactcg aacgtccagg
     2281 gttgggtgcg tcccgagttg gagatggtgc ggggccacct cgcccggggc accccgatcc
     2341 tggggatctg cttcggggcg caaagcctct ccgcggccct gggagggacg gtcagccact
     2401 cgaagcgacc ggagtacggc tgggtgagca tcgactcgaa ggtcgaggag atcgctgccg
     2461 ggccctggtt ccagtttcac cacgacgaat tcactttgcc c
//
LOCUS       DATITW010000068         1705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_3283, whole genome shotgun sequence.
ACCESSION   DATITW010000068 DATITW010000000
VERSION     DATITW010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3283"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VLS86_00880"
     CDS             complement(<1..561)
                     /locus_tag="VLS86_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HSO49072.1"
                     /translation="MPTVASIVSALAERTRPESAAAWDPVGLQLGDLSAQAESVAVCH
                     EVTEDVVAALEKEPVDLLVTYHPLLFEPVNRILAGRSPSARAFRLMTAKVNLVVTHTD
                     FDAAPGGTADALASLLQLRDATPFGSYEAEGRPAIGRVGLFEATLDALDARISDTFGS
                     NGLRVSGDRSRRLERVAVVPGSGAGLI"
     gene            639..1679
                     /locus_tag="VLS86_00885"
     CDS             639..1679
                     /locus_tag="VLS86_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HSO49073.1"
                     /translation="MDLTPSTTWAPPPDWARVVTIDSHTAGEPFRVVVGGTPPIPGAT
                     MIERRRFAQEHLDVLRRALMWEPRGHADMYGGLIGSPTDPSSDLSVLFIHNEGFSTMC
                     GHGIIALTKVVLDTGILPRTEPETVIRIDTPAGQIVATATVADGVVGNVRFRNVPSFV
                     VDLDNTVEVPGLGPVRYDLAFGGAFYAYVHAADLGIPLLPAATNLLIEAGRAIKEAVI
                     GSRTIPHPGEPDLGFLYGVIFVGPAEDPAHHSRNVCVFAEAEVDRSPTGTGVSGWLAI
                     HHARGELGVGDTVTIESIVGSTFTGTVIETTEFEGLAAVIPEVAGTAHITGRNELWLD
                     PDDVLGAGFLLR"
BASE COUNT          282 a          606 c          570 g          247 t
ORIGIN      
        1 gatgagcccg gcgccggatc cggggaccac tgccactcgc tccaagcggc gagagcggtc
       61 ccccgagacc cgcaacccgt tggagccgaa agtatcggag atccgggcgt cgagagcgtc
      121 cagggtggcc tcgaagagac cgacccgacc gatcgccggg cggccttctg cctcgtacga
      181 gccgaagggg gtcgcgtcgc gaagctggag cagggaggcc agcgcatcgg cggtgccccc
      241 cggagcggcg tcgaagtcgg tgtgagtgac gaccagattc accttggccg tcatcagccg
      301 aaacgcccgg gccgacgggg atcgaccggc caggatccga ttgaccggtt cgaagagcaa
      361 cgggtgatag gtgaccagca gatcgacggg ctctttctcc aacgccgcaa ccacgtcctc
      421 ggtgacctca tgacacaccg ccaccgactc ggcctgggcg ctcaggtcgc caagctgaag
      481 cccgaccggg tcccaggccg cggcggactc gggacgggtc cgctccgcca gagcggagac
      541 gatgctggca accgtcggca tgcgacgatg gtagagcgga ccgaacccac ccccgcgaag
      601 ggcgagcccg gcggggtggg ggcataggct ccctcgccat ggatctcacg ccgtcgacga
      661 cctgggcgcc tcccccggac tgggcgcggg tggtgaccat cgactcgcac accgccggcg
      721 agccgtttcg ggtcgtggtc ggggggacgc ctccgatccc cggcgccacc atgatcgaaa
      781 gacgccgctt cgcccaagag cacctcgacg tcttgaggcg ggcgctgatg tgggagccac
      841 ggggccatgc cgacatgtac ggagggctga tcggctcccc caccgatccc agcagcgacc
      901 tgtcggtcct cttcatccac aacgagggct tctccaccat gtgcggtcac gggatcatcg
      961 ccctgaccaa agtggttctc gacaccggga tcctgccccg aaccgagccc gagaccgtga
     1021 tccgaatcga cacccccgcc ggtcagatcg tggccacggc gaccgtggcc gatggtgtgg
     1081 ttggaaatgt ccgcttccgc aatgtcccct cgttcgtggt cgacctggac aacacggtcg
     1141 aggtgccggg gctggggccg gtgcgttacg acctggcgtt tggcggcgcc ttctacgcct
     1201 atgtccacgc cgcggacctg gggatcccac tgctccccgc agccaccaac cttctcatcg
     1261 aagcggggcg ggcgatcaag gaggcggtga tcgggtcgag gacgatcccc caccccgggg
     1321 agcccgacct cgggttcctc tacggcgtca tcttcgtcgg accggccgaa gacccggccc
     1381 atcactcgcg gaacgtctgc gtctttgccg aagccgaggt cgaccgctcc ccgaccggaa
     1441 ccggggtcag cggctggctc gccatccacc acgcccgcgg tgagctcggc gtcggcgaca
     1501 ccgtcaccat cgagagcatc gtgggcagca ctttcaccgg gaccgtgatc gaaacgaccg
     1561 agttcgaggg tctggccgcg gtgatccccg aggtggccgg gaccgcccac atcacgggtc
     1621 gcaacgagct ctggctcgac cccgacgacg tcctcggggc gggcttcctg ctccgctgac
     1681 cgctaccggg cgaccttcgc cctcc
//
LOCUS       DATITW010000069         1855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_724502, whole genome shotgun sequence.
ACCESSION   DATITW010000069 DATITW010000000
VERSION     DATITW010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724502"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VLS86_00890"
     CDS             complement(<1..824)
                     /locus_tag="VLS86_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660553.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HSO49074.1"
                     /translation="MTDGLSPRAGLSNRVRFVTATTLFDGHDASINIFRRILQASGAE
                     VIHLGHNRTGLDIVETAIEEDAHAIAVTSYQGGHMEFYRYVRDLLVERDAGHIRLFGG
                     GGGTILPTEGGELANYGITRIYSPDDGRELGLQGMVADMMTRSDFPTGELGDDFDLSA
                     LTTENTKVVAGLISAAENYSEKYADVFEQVEARATAAKTLVIGVTGTGGSGKSSLVDE
                     LVRRLLLDFEDLRLAVISVDPSKRRSGGALLGDRIRMNSLPHPRAYMRSLATRQANL"
     gene            920..1231
                     /locus_tag="VLS86_00895"
     CDS             920..1231
                     /locus_tag="VLS86_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49075.1"
                     /translation="MAYYLGRLQGSRLEPTNVTIEIDDGRFRITTGRRHMGSWPMRDV
                     KVERTSIYRFSFDIEGDHLEFFPDDPSEFSDAVGAFIDLTDSKRGRFGLKARIQHASG
                     S"
     gene            1257..>1855
                     /locus_tag="VLS86_00900"
     CDS             1257..>1855
                     /locus_tag="VLS86_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HSO49076.1"
                     /translation="MARHLIKSGAIAGAVVIGLEAAYAVLRPTPTLTEFDPSASFGDS
                     TSPQLRVVVLGDSSVTAPGVSGPEEVWVSLICERLAADHHVILDSLAVGGSCAHDLIQ
                     NQVGPALELDPDLILIAVGANDVIRGVTRKRFEMNLDHLVSELSSTRATIILSGVGDL
                     GTIPRLHPPLRQLFSHRSAGFDRIHRRVAAKYDAHVVEQR"
BASE COUNT          325 a          600 c          570 g          360 t
ORIGIN      
        1 aggttcgcct gccgggtggc gagcgagcgc atataggcgc ggggatgggg cagagagttc
       61 attcggatcc ggtccccgag cagggcgccg ccggagcgtc gcttcgacgg gtccaccgag
      121 atcaccgcaa gacggagatc ctcgaagtcg agaagcagcc tgcgcaccaa ctcgtcgacg
      181 agactcgact tccccgaccc accggtgccg gtgaccccga tcaccagcgt cttcgcggcc
      241 gtggcccggg cctcgacctg ctcgaagaca tcggcgtact tctccgagta gttctcggcg
      301 gccgagatca aaccggccac gaccttggtg ttctccgtgg tcaatgccga gaggtcgaag
      361 tcgtcgccca gctcaccggt cggaaaatcc gacctcgtca tcatgtcggc gaccatcccc
      421 tggagaccca gttcccggcc gtcgtcgggc gagtagatcc tggtgatgcc gtaattggcc
      481 agctcaccgc cctcggtggg caggatcgtc cctcccccgc ctccgaagag acggatatgg
      541 ccggcatccc tctcgacgag caggtctctc acatagcggt agaactccat gtggccgccc
      601 tgataggagg tgaccgcgat ggcatgggcg tcctcctcga tggcggtctc cacgatgtcg
      661 agaccggtgc ggttgtgacc gaggtggatc acctccgccc ccgaggcctg aaggatgcgg
      721 cggaagatgt ttatcgacgc atcgtgcccg tcgaacaggg tggtggcggt gacgaatcgg
      781 actcggttgc tcagacccgc tcgcggtgag agaccgtcgg tcatgttctc cttcggggac
      841 tagtccaaac tgtaccggca gattcgggcg ctttttctcg tttcaacatg acgtgaacac
      901 cgctagattg tgttttggga tggcttatta cctagggcgg ctacaaggct ccagactgga
      961 gccaacaaac gtgacgatcg aaatcgacga tgggcgattc cgaatcacca ccgggcgacg
     1021 gcacatgggg tcatggccga tgcgtgatgt caaggtagag cgaacctcta tctaccgatt
     1081 ctccttcgac atcgagggcg atcatctgga gttcttcccc gacgatccat cggagttctc
     1141 agacgccgtt ggcgcattca tcgacctgac ggacagcaaa cgcggtcgct tcgggctcaa
     1201 ggcacgtatc caacacgcat ccggcagttg atcctgccgt ctggcagtat cacgccgtgg
     1261 ctcgccatct gatcaagagt ggagcgattg ccggcgcggt cgtcatcggt ctggaagctg
     1321 cctacgcggt gctccggccc accccgaccc tcaccgagtt cgacccgtct gcgtcgttcg
     1381 gtgactcgac atcgcctcaa cttcgggtcg tggttctcgg cgattcttcg gtgacggcac
     1441 cgggtgtctc cggccctgag gaggtgtggg tttccctgat ctgtgaacgc ctcgccgccg
     1501 accaccatgt catcctcgac tccttggccg tcgggggatc ctgcgcccac gacctgatcc
     1561 agaaccaggt cggcccggcc ctcgagctcg acccggacct gatcctgatt gcggtggggg
     1621 cgaatgacgt gatccgcggt gtcactcgca agcgattcga gatgaacctg gatcacctgg
     1681 tcagcgagct ctcgtcgacc cgggccacga tcatcctcag cggggttggc gacctgggga
     1741 cgattccccg gctccatcct cccctccgac agctgttctc ccaccgctcg gccgggttcg
     1801 acaggatcca tcgtcgggta gcggccaagt atgacgccca cgtcgtcgag caacg
//
LOCUS       DATITW010000070         5897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_324095, whole genome shotgun sequence.
ACCESSION   DATITW010000070 DATITW010000000
VERSION     DATITW010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324095"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VLS86_00905"
     CDS             <1..282
                     /locus_tag="VLS86_00905"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HSO49077.1"
                     /translation="IGSASLRDQMSAGINRLLVGLVLEDKGIPRHGYRVRSEEGEGQV
                     TSGNLSPMLDRGIALAYLSPPPSPEAPIEVEIRGRWVPGRVARPPFHKR"
     gene            285..788
                     /locus_tag="VLS86_00910"
     CDS             285..788
                     /locus_tag="VLS86_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012832683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSO49078.1"
                     /translation="MEPRISGRPLMPEGYGVKDDGPFLEWKEVAVRLVDANEYWLAST
                     RPDGRPHVIPRWGVWLDNHFWYDGSPLTRHVQNLERNPACALHLESGTRVTIVEGISE
                     ASRPIVGELGERLAAEYSRKYKDQGYAPTPDAWSDEVAGGMRVLTPNKAIAWSQFPTD
                     LTRFAFD"
     gene            836..1636
                     /locus_tag="VLS86_00915"
     CDS             836..1636
                     /locus_tag="VLS86_00915"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HSO49079.1"
                     /translation="MSRPVPVGDIGMELVREWVPETAPRGHLVLVHGIAEHCGRYERT
                     GDLLAAAGFFVRSFDLIGFGGSGGRRGDINVWSRYHDQIQRHMEWAKAQGGPVILMGH
                     SMGGNLALGYVLSGRAQPDLLVLTAPALGGGAGWQKALAGVASRIAPTVAIPNSLKGE
                     QLSRDPEVGEAYFSDPLVYAKATPRLGAALFNAMEELTEDAPQLAVPTLVLHGTADTI
                     VPPQSSAVLGDLPNVERRLLPNLRHEILNEPEGPELVQEIIVWIDAHL"
     gene            1640..2035
                     /locus_tag="VLS86_00920"
     CDS             1640..2035
                     /locus_tag="VLS86_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HSO49080.1"
                     /translation="MPLPRRLARFNKVVTNRIAGPLASRVPAMAVVVHRGRISGNEYR
                     TPVNCWIDDRTAIVALTYGRGTDWLKNLTAAGGGTVVARGRSHRVGKPELIGVEGMVR
                     MPRIVRGILGLIDVTEFAELPLLSRAQPT"
     gene            complement(2013..3047)
                     /gene="tdh"
                     /locus_tag="VLS86_00925"
     CDS             complement(2013..3047)
                     /gene="tdh"
                     /locus_tag="VLS86_00925"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HSO49081.1"
                     /translation="MRALVKHADEIGLRLEDVPIPEPGHDDILIRVFTTGICGTDLHI
                     FNWDDWARTTIPTPMTVGHEFSGEIVAIGSNVSGFEVGELVGAEGHVVCNRCRNCLAG
                     RRHLCQDAHGIGVNLPGAFADYVLIPKSNVWRHHEGLDPEIAALFDPFGNAVHTALEF
                     DVLGEDVLITGAGPIGLMAVAVVAHAGARNVVITDVNPFRLDLAKKMGATRAVDVRSV
                     DLHDIQIELGMTEGFDVALEMSGAPSAMEDILLNTIHGANVAILGIPADPFPIDWSIV
                     IFNMLNLKGIYGREMFETWYKMNVLVESGVDISPVITHRFEVEEFEAAFEATASHDSG
                     KVLIRWAEPD"
     gene            3177..3704
                     /locus_tag="VLS86_00930"
     CDS             3177..3704
                     /locus_tag="VLS86_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HSO49082.1"
                     /translation="MTEYLAGNLLVATPLLVDPNFWRTVVLLLLHDGESTIGVVLNRP
                     TQELVETHIPVWGEVSAPPGLVHFGGPVEPQVAIGLATGQTGEPTGVPGLSIVDLDSS
                     PGPELGLVRVYSGYAGWGPGQLEAEIEDGSWYVVPASPDDPFDRPEGQWSRVLRRQQG
                     HLGLVSTFPDDVSLN"
     gene            3744..4445
                     /locus_tag="VLS86_00935"
     CDS             3744..4445
                     /locus_tag="VLS86_00935"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HSO49083.1"
                     /translation="MTTTAPKLRPSLKRKAPGLLVERGLRLRGLDVIVGIDEVGRGSW
                     AGPLTVGAAVVPLDRRVYKIRDSKLLTEDEREALFDRIAGWCERWSVGHASPEECDRL
                     GMSDAQRLAAKRAIGGLGIVPDHVLVDGTWDFVGGATRIVRGDRTSLSIAAASILAKV
                     TRDRIMRDLANHHPGYNFEANKGYPCPIHKAALQAWGPSSIHRKSWVFMDHLMWNGLE
                     RYHRPEPQLLLPFET"
     gene            4614..5633
                     /locus_tag="VLS86_00940"
     CDS             4614..5633
                     /locus_tag="VLS86_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSO49084.1"
                     /translation="MNGIEELNAFFRRMPVAFYRTSATGKLIAANPALANILGFDSVE
                     EMMSRLETVESVYMDPRQRTEWLDRIAREGVILDFDVQLSRPDGKPTWVRDSARAIRD
                     ADGEIQYYEGSLVDVTDKIEAQKDKDDFIATVSHELRNPLAAMLGLGQELADHYDSFS
                     DEERRDMAQVIARQADDASWLIEDLLVAYRANLSQVSVTLQEFDVTKEIERVLEVVDH
                     PIQLKVFQGSPRLLADPRRTRQILRNLVSNAIRYGGDDIQVSIAKAGDRLEVKVQDSG
                     ERIPHADVERIFRAYERGRGPSHSASVGLGLNVARRLARLMDGDLTYRYEDEWSSFVL
                     SLPSA"
     gene            5661..>5897
                     /locus_tag="VLS86_00945"
     CDS             5661..>5897
                     /locus_tag="VLS86_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSO49085.1"
                     /translation="MRVLVTGGAGFIGSHTVVSLIEAGHEPVIVDNLSNARRSVVKRI
                     GQVVGTEPEFHEADVRDVDAVTKILQSGFDACIHF"
BASE COUNT         1044 a         1874 c         1959 g         1020 t
ORIGIN      
        1 atcggcagcg caagtctccg cgaccagatg tctgccggca tcaaccgatt gttggtcggg
       61 ctggtgctcg aggacaaggg gataccccgc cacggatacc gtgtgcgatc cgaagagggc
      121 gagggtcagg tgacctcggg caacctgagc ccgatgctcg acagggggat cgccctggcg
      181 tacctgagcc ctcccccctc cccggaggct ccgatcgagg tggagataag aggtcgttgg
      241 gtgccgggtc gggtcgccag gcccccgttt cacaagaggt gatcatggaa ccacgaatca
      301 gcggccgtcc cctgatgccg gaggggtacg gggtcaaaga cgatggcccg ttcctggaat
      361 ggaaggaggt cgcggtccgg ctggtggatg ccaacgagta ctggctggcc agcaccagac
      421 ccgacgggcg acctcatgtc attccccggt ggggagtctg gctggacaac catttctggt
      481 acgacggatc accactgacc cgccacgtcc agaatctcga gcgcaacccg gcttgcgccc
      541 tccacctcga gagcggcaca agggtgacca tcgtcgaagg gatctccgaa gcctcccgac
      601 ccatcgtcgg ggagttgggg gagcgtctcg ccgccgagta ctcgaggaag tacaaggacc
      661 agggctatgc ccccaccccc gacgcctggt cggacgaagt ggcgggaggg atgcgggtgc
      721 tgacaccgaa caaggccatt gcctggtcgc agttccccac cgacctgacc cgctttgcgt
      781 tcgactgatc ctccgtagga tctgactttc acaggaggag ttgattttgc ccagtgtgtc
      841 tcgcccggtg ccggtcggcg acatcggaat ggagttggtc agagaatggg ttccggagac
      901 cgcgccccgg ggccatctcg tgctggtcca tgggattgcc gagcactgtg gtcgctacga
      961 gcgaaccggt gacctgctgg ctgcggccgg tttcttcgtt cgttctttcg acctcatcgg
     1021 cttcgggggg tctggcggga gacgcgggga tatcaacgtg tggtcccgat accacgacca
     1081 gatccaacgc cacatggaat gggccaaggc ccagggtggg cccgtcatcc tgatgggtca
     1141 ctccatgggg ggaaatctcg ccctcggcta cgtgctctcc ggccgggccc aacccgatct
     1201 cctcgtcctg actgcaccgg ccctcggcgg cggagccgga tggcagaagg ccctggccgg
     1261 ggtcgcgtcc aggatcgcgc ccaccgtggc catccccaac tcgctcaagg gcgagcagct
     1321 gtcccgagac cccgaggtcg gtgaggccta cttctccgac ccgctggtgt acgccaaggc
     1381 gaccccccgg ctcggagctg ccctgttcaa cgccatggag gagctcaccg aagatgcacc
     1441 acagctcgcg gtgcccactc tcgtgctcca cggcactgct gacaccatcg tcccgccaca
     1501 gtcatcggcg gtcctgggcg acctgcccaa cgtcgagcgg agactgttgc caaatctccg
     1561 gcacgagatc ctcaacgagc cggagggccc cgaactggtc caggagatca tcgtctggat
     1621 cgatgcccac ctctgagcga tgccgctgcc acgccgactc gctcgcttca acaaggtggt
     1681 caccaaccgc atcgccggac cactggcctc ccgggtcccg gccatggcgg tcgtcgtcca
     1741 cagaggcagg atctcgggaa acgagtatcg gactccggtg aactgctgga tcgatgatcg
     1801 gactgccatc gtcgccctga cctacgggag aggcaccgac tggttgaaga acctcaccgc
     1861 cgccggaggc ggcactgtcg tggcgagggg gaggtcgcat cgggtcggca agccggagct
     1921 gatcggcgtg gagggcatgg tccggatgcc gcggatcgtc cgagggatcc tcggcctgat
     1981 cgacgtcacc gagttcgcag agctgccgct gctcagtcgg gctcagccca cctgatcagc
     2041 accttgcccg agtcgtggga ggcggtggcc tcgaaggcgg cctcgaactc ctccacctcg
     2101 aagcgatggg tgatgaccgg tgagatgtcg acccctgatt cgacgagcac gttcatcttg
     2161 taccaggtct cgaacatctc ccggccgtag atgcccttca ggttgagcat gttgaagatg
     2221 acgatgctcc aatcgatggg gaacgggtcc gccggtatcc cgaggatggc gacgttggcg
     2281 ccatggatcg tgttcaggag gatgtcctcc atggccgacg gggcccccga catctcgagg
     2341 gcgacgtcga agccttcggt catccccagc tcgatctgga tgtcgtggag atcgaccgac
     2401 ctgacatcga cggcgcgcgt cgcgcccatc ttcttggcca ggtcgagacg aaacgggttc
     2461 acgtcggtga tgaccacatt gcgggcaccg gcgtgggcca caaccgccac ggccatgaga
     2521 ccgatggggc cggcccccgt gatcaggacg tcttcaccca gcacgtcgaa ctcgagggcg
     2581 gtgtgaaccg cattgccaaa gggatcgaag agggcggcaa tctcggggtc gaggccttcg
     2641 tggtggcgcc agacattcga tttggggatc agcacgtagt cggcgaatgc cccggggagg
     2701 ttgacgccga tcccgtgggc gtcctggcac agatgccgtc ttcccgccag acagttgcga
     2761 caccgattgc acaccacgtg gccctcggcg cccaccagct cgccgacttc gaaaccggac
     2821 acgttggacc cgatggcgac gatctcaccg gagaactcgt ggccgaccgt catcggggtg
     2881 ggaatggtgg tccgcgccca gtcgtcccag ttgaagatgt ggaggtcggt gccgcagatc
     2941 ccggtggtga agacccggat caggatgtca tcgtggccgg gctcggggat ggggacatcc
     3001 tcgagacgca ggccgatctc gtcggcgtgc ttgacgagtg ccctcactcc gaccgctccc
     3061 cggacgagga ggctgtcgaa ttcgatggct gatggtcgtt gctcgggctc acgatgcgcc
     3121 tcccacgata aagccccgag gtcgcggaat gggacacgct gtggcacgat gcagccgtga
     3181 ccgaatatct cgccgggaat ctgctggtgg ccacgccgct cttggtagac cccaatttct
     3241 ggcggaccgt ggtgctcctg ttgctgcacg acggtgagtc aacgattggc gtcgtcctga
     3301 atcgcccgac tcaggaactc gtcgagaccc acatcccggt ctggggtgag gtctccgccc
     3361 cacccggtct ggtccacttc ggcggaccgg tggagccgca ggtggcgatc gggctggcca
     3421 ccggtcagac cggagagccc acgggagtcc ctggtctgag catcgtcgat ctcgactcgt
     3481 cgccgggccc cgagttgggg ttggtgaggg tctactccgg gtacgccggt tgggggccgg
     3541 gccagctcga ggccgagatc gaggacgggt cctggtacgt cgtgcccgca tccccggacg
     3601 acccattcga tcggccggaa gggcagtggt cacgggtgct gcgacgtcag caggggcatc
     3661 tcggcctggt ctcgaccttt cccgatgatg tctcgctcaa ctgaaccact gacgggtcct
     3721 ccccggacct accctgatcg ccaatgacca ccaccgcccc caagctccgt cccagcctga
     3781 agcgcaaggc gcccgggctc ctggtagagc gggggctgcg gctgagaggg ctcgacgtca
     3841 tcgtcgggat cgacgaggtg gggcggggct cctgggccgg tccgttgacg gtcggggccg
     3901 cggtggtccc tctcgatcga cgggtgtaca agatccgcga ctcgaagctg ttgaccgagg
     3961 acgaacgtga ggcgttgttc gaccggatcg cgggttggtg cgagaggtgg tcggtgggcc
     4021 acgccagccc ggaagaatgc gaccggctgg gcatgtcgga cgctcagcgt ctggcggcga
     4081 agcgggcgat cggaggtctc gggatcgtgc cggatcatgt cctcgtcgac ggcacttggg
     4141 acttcgtcgg cggtgccacc cgaatcgtca ggggtgaccg gacctcactg tcgatcgccg
     4201 cggcctcgat cctggccaag gtgacccggg accggatcat gcgtgatctg gcgaatcacc
     4261 acccgggcta caacttcgag gccaacaagg gctatccgtg cccgattcac aaggccgcgc
     4321 tccaggcgtg gggtccctcg tcgattcacc gcaagtcgtg ggtgttcatg gatcatctga
     4381 tgtggaacgg cttggagcga taccaccggc ccgagccgca gctgctcttg ccgttcgaaa
     4441 cctgactccc gggggtatgg gtgaaggtcg cgacaacttc aggtcgtgag gtcgggcagg
     4501 aacctggagg atcgaccaca atcaagtgac ttcggttccc gcacgaagac agtgctcagg
     4561 gcagggcgac cgatctgtga agaaaggccg gcgcgttgcc gatagacacc gaaatgaacg
     4621 gcattgaaga gctgaacgcc ttcttcaggc gcatgccggt tgccttctac cgcacttcgg
     4681 ccacgggcaa gctgatcgcg gccaaccccg ccctggccaa catcctcggc ttcgactcgg
     4741 tggaggagat gatgagccgg ctggagaccg tggaatctgt ctacatggat ccccggcaga
     4801 gaaccgaatg gctggatcgg atcgcaaggg agggtgtcat cctcgacttc gacgtgcagc
     4861 tgtccagacc cgacgggaag ccgacctggg tccgtgactc ggcccgggcc atccgcgacg
     4921 ccgacgggga gatccagtac tacgaagggt cgctcgtcga cgtcaccgac aagatcgaag
     4981 cacagaagga caaggacgat ttcatcgcaa ccgtgtcgca cgagctgcgc aaccccctgg
     5041 ccgcgatgct cggcctcggc caggagctgg ccgatcacta cgactcgttc agcgacgagg
     5101 agcgacggga catggcccag gtcattgcca gacaggccga cgatgcctcc tggctgatcg
     5161 aagacctcct cgtggcctat cgggccaacc tgagccaggt gtcggtgacg ctccaggagt
     5221 tcgacgtcac caaggagatc gagcgggtcc tggaagtggt cgaccacccg atccagctca
     5281 aggtgttcca gggctctcca cgactccttg ccgatcccag gcgaacccgc cagatcctgc
     5341 gcaacctggt ctccaatgcg atcaggtacg gaggtgacga catccaggtc tccatcgcca
     5401 aggccggcga caggctcgag gtcaaagtcc aggactcggg cgagcgaata ccccatgccg
     5461 acgtcgagcg gatcttccgg gcctacgagc gggggagagg gccgtcgcat tcggcatcgg
     5521 tggggctcgg gctcaacgtg gcgcgccgtc tggcccggct catggatggc gatctgacct
     5581 accgttatga agacgagtgg tcgagcttcg tcctctccct cccgtcagcg tgactccggg
     5641 gcggtcctac catgccccgg atgcgtgttc tagtaaccgg tggcgccggg ttcatcggca
     5701 gccacaccgt ggtttcactg atcgaggccg gtcacgagcc cgtcatcgtg gacaatctgt
     5761 ccaatgcccg gcgctcggtg gtgaagagga tcggacaggt tgtcggcacc gagcccgagt
     5821 tccacgaggc cgatgtcagg gacgtggacg ccgtcaccaa gatcctccaa tccggcttcg
     5881 acgcctgcat tcacttt
//
LOCUS       DATITW010000071         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_724577, whole genome shotgun sequence.
ACCESSION   DATITW010000071 DATITW010000000
VERSION     DATITW010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724577"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..755
                     /locus_tag="VLS86_00950"
     CDS             237..755
                     /locus_tag="VLS86_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSO49086.1"
                     /translation="MDPEVLQRFRVGDEAAVKAVYDRFGGSVFALSMSILGEHGLAAD
                     ATQQTFIKAWRAASTYDPERSFAPWIYAIARRTAIDIYRKQIRVVVSDEIEIAVYPPG
                     LETAWEVFEVRSALDRLPDEERQVVKLSHLDGFTHAEISEQLDIPVGTVKSRSHRAHQ
                     RLAELLRHVEEA"
     gene            798..1448
                     /locus_tag="VLS86_00955"
     CDS             798..1448
                     /locus_tag="VLS86_00955"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HSO49087.1"
                     /translation="MMASDETRAQDLASGSGDAPRDYERLDLIRGVLAGEATWVEPPP
                     AVVDALLAAVAHEAPGSADGAVGAAPARWPLVVAAVGTVAAAVALVLSTISVIDAQQE
                     TVVAMEGTELEVGAWGQAAVRPTESGWWILLDLKGLPPAPEGSFYEGWVWSDDGDGVS
                     IGTFHLRGGDAPVVLWSGVEMADYPSIWVTLEHEDEGAEASDLVIMTGRMPDAPEA"
     gene            complement(1457..1684)
                     /locus_tag="VLS86_00960"
     CDS             complement(1457..1684)
                     /locus_tag="VLS86_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HSO49088.1"
                     /translation="MRVSSLLIAVGVGIALIGIVLRYFPSAFSWFGNLPGDIKYESET
                     GRVFIPLTSMLVVSVVATVIINLAGRLFRDG"
     gene            complement(1688..2239)
                     /locus_tag="VLS86_00965"
     CDS             complement(1688..2239)
                     /locus_tag="VLS86_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSO49089.1"
                     /translation="MLERAQLRAEQTLLVVGVGGGVSAAAMHLGLAFRANVYVTSRSQ
                     EKIDWAVEGGATGGFDSGSDFGVEMTALGGADVVVDNVGPATMRQSMRAAKNGGRVMI
                     CGSTSGPKLELTVPYLFFRQLELIGSSMATHGQFARAVTWVTTEGADSPVGKVFDFAE
                     LPAALEYLEAGDQVGKVVLRHPD"
     gene            complement(2288..2704)
                     /locus_tag="VLS86_00970"
     CDS             complement(2288..2704)
                     /locus_tag="VLS86_00970"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSO49090.1"
                     /translation="MKAWQLHDTRGIESYELGDVEEPEPGPGEVRIKISHSALNHLDL
                     WVSQGLPAPKHFPHTTGADGAGYVDAVGEGASGFDIGDEIIIDPSISCGQCVHCHNDD
                     IVYCDTFQILGEHRPGTFTEKIVITTINVVRNPESL"
     gene            2746..3180
                     /locus_tag="VLS86_00975"
     CDS             2746..3180
                     /locus_tag="VLS86_00975"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HSO49091.1"
                     /translation="MTVRVPTDREVHTTSVRVRFYELDPYDHVNHTVYFSYFETARID
                     YLAEMGWGLDRLKEQGWQFVVVELSARFQAAARFGEELTIHTWIEEVGRVKSTWQQVM
                     LRGGEVVARLTISAAFTDLLGKPRRIPEEFIRHMDSLRAPAP"
     gene            complement(3294..>3554)
                     /locus_tag="VLS86_00980"
     CDS             complement(3294..>3554)
                     /locus_tag="VLS86_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HSO49092.1"
                     /translation="GSESHKAAVIGDPIGAPFKDTAGPALTPLIKVMNLISLLILPAV
                     IALSDSSARYVIAGVSLVILLTAITWSKRKTDVGAMTSELVS"
BASE COUNT          607 a         1130 c         1192 g          625 t
ORIGIN      
        1 atcatcgtcg gccacggtgg tatctggagc ggctgcggtc gtcgtggcct cgtcggcagg
       61 cgccgtggtc gtggtatccc cgccctcgtc ggcgtcggtg gcacaggcgc tgagcaccag
      121 catcgctgcc atgagcagag cccagactcg gatcttcatc atgtgtctcc tcggttcatc
      181 gtctcctcat tacgcatcgg ggatgcgcat ggttcgcgac ctggcacaat ccggttgtgg
      241 accccgaggt tctgcaacga ttccgggtcg gtgacgaagc ggcggtgaaa gccgtttacg
      301 accgattcgg aggctctgtg ttcgccctct cgatgagcat cctgggtgag cacggtcttg
      361 ccgcggacgc gactcagcag accttcatca aggcttggcg cgcggcttcg acctacgacc
      421 cggagcggag cttcgcccca tggatctacg ccatcgctcg ccggaccgcg atcgacatct
      481 accgaaagca gatccgggtg gtggtcagtg acgagataga gatcgccgtc tacccgccag
      541 gcctggaaac cgcatgggaa gtgttcgagg tccgctccgc cttggatcgg ctgccggacg
      601 aggagcgaca ggtggtcaag ttgagccatc tcgacgggtt cacccacgcc gagatctccg
      661 agcagctcga catcccggtg gggacggtca agtcccgatc gcatcgggct caccagcgac
      721 tggccgagct gctgaggcat gtggaggaag catgatctcc gcgaaccgcc ggatgtatcg
      781 ggacgtacag ggtgtgaatg atggcctctg atgaaacgcg cgcgcaggat ctggcctcgg
      841 gctcaggtga tgcgccgcgc gattacgagc gactcgacct gatcaggggg gtgctcgccg
      901 gcgaggcgac ctgggtcgag cccccacctg cggtggtcga cgctctcctg gccgcggtcg
      961 cacacgaggc cccgggttcg gccgacggcg ctgtcggggc cgcccctgcc cggtggccgc
     1021 tggtcgtcgc tgccgtgggc acggtggccg cggcagtggc cctggtcttg tccacgatca
     1081 gcgtcatcga cgcccagcag gagaccgtgg tggcgatgga agggacggag ctggaggtcg
     1141 gggcatgggg ccaggccgcg gtccgcccca ccgagtcggg ctggtggata cttctcgacc
     1201 tgaaaggtct gccaccggcc ccggagggga gcttctacga aggttgggtg tggagcgacg
     1261 acggcgacgg tgtcagcatc gggaccttcc atcttcgcgg tggcgacgcc ccggtggtcc
     1321 tgtggtccgg tgtcgagatg gcggattacc cctcgatctg ggtcactctg gagcacgagg
     1381 atgaaggagc cgaggcctcc gacctggtga tcatgaccgg ccgtatgccg gacgcacccg
     1441 aggcctgatc caccgctcaa ccgtcacgga acagcctgcc cgccaggttg atgatcaccg
     1501 tcgccaccac gctcaccacc agcatcgagg tcagcgggat gaatacccga cccgtctccg
     1561 actcgtactt gatgtcgccc gggaggttac caaaccagga gaaagccgaa gggaagtagc
     1621 ggaggacgat cccgatcagg gcgatcccca ccccgactgc gatgaggagg ctggagactc
     1681 gcaacggtca gtccggatgg cgcaggacga ccttgcccac ctgatctcct gcttcgaggt
     1741 attcgagagc ggcgggcagc tctgcgaagt cgaagacctt gcccaccggg gagtcggccc
     1801 cctcggtggt gacccaggtg acggcacgcg cgaactggcc gtgggtggcc atcgacgacc
     1861 cgatcagctc cagctggcgg aagaagagat agggcaccgt caactccagc ttgggcccgc
     1921 tggtcgagcc gcagatcatg acccgcccgc cgttcttggc ggctcgcatc gactggcgca
     1981 tcgtcgcggg gcccacattg tcgaccacga catcggcgcc acccagggcg gtcatctcca
     2041 ccccgaagtc gctcccggaa tcgaagcctc ccgtcgcgcc tccttccacg gcccagtcga
     2101 tcttctcctg ggaccgcgag gtgacataga cattggccct gaaggccaga ccgaggtgca
     2161 tcgcagcagc cgagacgcca ccacccactc cgaccaccaa cagggtctgc tctgctctca
     2221 gttgggctcg ttccagcatg cgcagggcgg tggcggtgga caggacgaat gaccctgcga
     2281 cttgccatta caacgactcc ggattgcgta ccacatttat ggtcgtgatg acgatcttct
     2341 cggtgaaggt gccgggccgg tgctcgccga ggatctgaaa cgtatcgcag tagacgatgt
     2401 cgtcgttgtg gcagtggaca cactgtccgc aggagatcga ggggtcgatg atgatctcgt
     2461 cgcctatgtc gaagccactc gccccctcgc ctaccgcgtc cacataccct gccccgtcgg
     2521 ccccggtggt gtgagggaag tgcttggggg cgggcagccc ctgggacacc cagaggtcga
     2581 gatgattgag ggcggagtgg ctgatcttga tccgaacctc acccggcccc ggctccggct
     2641 cctcgacatc gccgagctcg tacgactcga tcccccgggt gtcgtgaagc tgccaggcct
     2701 tcatgttggc gagggtagcc tcgaccctgt gagcggagga gagagatgac cgtccgggtg
     2761 ccaaccgacc gcgaggtcca caccacatcg gtcagagtcc gtttctacga gttggatcct
     2821 tatgaccacg tcaatcacac cgtctacttc tcctatttcg aaacggcgcg tatcgactac
     2881 ctggccgaga tgggctgggg tctggaccga ctcaaggagc agggctggca gttcgtcgtc
     2941 gtcgagctca gcgcccggtt tcaggcagcc gctcgctttg gcgaagagct gacgatccac
     3001 acctggatcg aggaggtggg ccgggtgaaa tccacctggc agcaggtcat gctccggggc
     3061 ggggaggttg tggcccggct cacgatctcg gctgccttca ccgatctcct cgggaagccg
     3121 cgacggatcc ccgaggagtt catccgtcac atggacagct tgagggcccc ggcgccctga
     3181 gccgttgagg gccagctgcc tggctccccg taattgggac cgtcaggggt cagatacgga
     3241 cctgtcggtc ccaatttcgg attgggggcc cccgccgccc tgagcgctga gggctagctg
     3301 accagctccg aggtcatggc gccgacatct gtcttgcgct tcgaccaggt gatggctgtg
     3361 agcaggatga cgaggctgac cccggcgatg acgtagcgcg ccgacgagtc gctgagggcg
     3421 atcacggcgg ggaggatcag gagcgagatc aggttcatca ccttgatcag cggggtgagg
     3481 gcgggcccgg ccgtgtcctt gaacggggcg ccgatggggt ccccgatcac cgccgccttg
     3541 tgcgactctg agcc
//
LOCUS       DATITW010000072         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_324150, whole genome shotgun sequence.
ACCESSION   DATITW010000072 DATITW010000000
VERSION     DATITW010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324150"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1026
                     /locus_tag="VLS86_00985"
     CDS             184..1026
                     /locus_tag="VLS86_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49093.1"
                     /translation="MTREVSAPESTATETIKRIGPPLVVGSVLGSTLGIVVAQFADDL
                     GDGILAVILGGNAIVYNNRVEITGASDLAWAGGFLLCLIIGFLALFTYPTARGHGVAR
                     LTLLWMVLHLLRQAFAQAVLLPLDSTSDLALAYETLDAPPGLDVVIAAGGGVGLLLIA
                     LSAASAFLAFAPHHRMISTGRRRFTFAFWIALVPAVAAAFIAIPFFLPDAGSGVVPSL
                     PLTAVIFLATVAAAPGTTTVFGPEDTRETPWPWGLGATLVVILLVQLLVFAGGVSVDP
                     TRWG"
     gene            1023..1502
                     /locus_tag="VLS86_00990"
     CDS             1023..1502
                     /locus_tag="VLS86_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HSO49094.1"
                     /translation="MTIHTAHVITVSDGVNAGAREDRSGDALARVLSDAGFAVLRSVV
                     PDEEAAIIAAIHEVTPVARLVVTTGGTGFGPRDVTPEATALALEREAPGLTHLMMARG
                     LESTTMAALSRGRAGSVGSTLVVNLPGSTKGAVESLEAIMEVIPHALDLLAGDTEHR"
     gene            1562..2119
                     /locus_tag="VLS86_00995"
     CDS             1562..2119
                     /locus_tag="VLS86_00995"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSO49095.1"
                     /translation="MKIEHEFTVARPPDVVWEFFQDVPSVAQCLPGAELAGQSDDGSY
                     EGKLSVKLGPMAASFEGKAVVTPDPATKTALIEGKGVDRSGGSRGQVKVNYSIVPDGA
                     GSRVGVDADVTLSGPVAQFGRTGLINEMSKRLIGEFVECLEAKLTAVTPVEAAAIEAG
                     EVKGISLFFASLWSWLRGLFRRGET"
     gene            2122..2493
                     /locus_tag="VLS86_01000"
     CDS             2122..2493
                     /locus_tag="VLS86_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HSO49096.1"
                     /translation="MRRIRLGLVCLLLLVSCGGEGGASITGDDVVVGMFDNRYEYTEI
                     QIPVGGSVTFRGAGRNPHNAVAADSSWSTEDVFGSLEQLEGDEATLTFDAPGEYVFFC
                     TFHGNDEGDGMAGTLTVGDSG"
     gene            2493..>3729
                     /locus_tag="VLS86_01005"
     CDS             2493..>3729
                     /locus_tag="VLS86_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSO49097.1"
                     /translation="MKRGWALLVAGAALSLASCAGPPFGDPSPAALGDPATDSPSDWS
                     GETRLVPQGYPTIQAAVDAAGPGDLILIDRGVYREEVEVTTPGITIRGVDRNQVVIDG
                     EFQRPNGIEVFLADGVVVENLTVRNATSNGVFWTGVRGYRGSYLTAVNNGVYGIYAFD
                     SSDGLLDHSYASGSPDAGFYIGQCDPCQSTITDSISEWNGLGYSGTNASTDLYIVNSV
                     FRHNVAGIAPNTLDGELLPPFHGVNVVGNLVHDNGNVEAPALAFEWAAQGNGILLAGG
                     GDSYVSRNRVLNHPQSGIVVLPMLDANFYMSYDHRVVGNHVAGSGLADLTLGGPATSG
                     NCFEDNVYDTSLPAALEFKQPCDGIRFPALFEIGSTTAQLGRLFESGLGLQPEVDITS
                     LPVPEPQPPMPDGAGAPVLP"
BASE COUNT          576 a         1201 c         1247 g          705 t
ORIGIN      
        1 cttctctcga tctggtgcgc gttgaattgg cgtcggacat ggcctgtgtt gtccggggca
       61 atcgcctatt tcctgatctg ggcgattggg acctcgatcg ttgcctcctg aggcttcccc
      121 ccgtatcgtt ctgctgacga cttaccggat ccggttcacc cccgtcgtta gcatccccca
      181 cacgtgacca gagaagtgtc cgcaccagag tcaaccgcaa ccgagacgat caagagaatc
      241 gggcctccac tggtcgtcgg ctcggtgctc ggctcgacgc tgggcatcgt cgtcgcccag
      301 ttcgccgacg atctcggcga cgggatcctg gcggtcatcc tcggtggcaa tgcgatcgtc
      361 tacaacaacc gggtcgagat caccggggcg tcggatctgg cctgggccgg tggttttctc
      421 ctgtgtctga tcatcgggtt cctcgccctc ttcacctacc cgacggcccg gggtcacggc
      481 gtcgcccggc tcaccctgct gtggatggtg ctgcatctgc ttcgtcaggc attcgcgcaa
      541 gccgtgctcc tgccgctgga cagcacgagc gacctggccc tggcgtacga gacgctcgac
      601 gcgccacccg ggctcgatgt ggtgattgcc gccggtggcg gggtcggcct gcttctgatc
      661 gccctgtcgg cagcgtctgc gttcctggca ttcgctcctc atcaccggat gatctccacc
      721 ggtcgcaggc gattcacatt tgccttttgg atcgcgctgg tgccggcggt tgctgcggcg
      781 ttcatcgcca ttcccttctt cctccccgac gcaggcagcg gggtggtgcc ctcattgccc
      841 ctgaccgccg tgatcttcct ggcgaccgtg gccgcggctc cggggaccac caccgtgttc
      901 gggcccgagg acacgcgcga gacgccatgg ccctgggggc tgggtgcgac cctggtggtg
      961 atcctgctcg tccagttgct cgtcttcgcc ggcggggtct cggtcgatcc cacccgctgg
     1021 ggttgaccat ccacacggca cacgtgatca ccgtcagcga cggggtgaat gcaggggcca
     1081 gggaggatcg gtccggcgac gccctggccc gggtgctgtc cgatgcaggc ttcgcagtcc
     1141 tccgcagcgt ggtcccggac gaggaagcag cgatcatcgc cgcgatccat gaggtgactc
     1201 ctgtcgctcg cctggtggtg acgaccggag gaaccggttt tggcccccgt gacgtgacac
     1261 cggaggcgac cgctctggcc ctggagcgag aagcccccgg tctcacccac ctgatgatgg
     1321 cgagaggcct cgaatcgacg acgatggctg cattgtccag ggggcgtgcc gggtcggtcg
     1381 gctcgacgct ggtggtgaat cttcccggat ccaccaaggg agccgtggag agtctcgagg
     1441 ccatcatgga ggtgatcccc cacgccctgg acctgctggc cggggacacc gagcatcgat
     1501 agggaaaacc aggggcgagt cactaccgtg cggcggccct ggttcggggg cgaggagagc
     1561 tatgaagatc gagcatgagt tcacggtggc gaggccacca gacgtcgttt gggagttctt
     1621 ccaagacgtg ccttctgtcg cccaatgcct gcccggcgcc gagctcgcgg gacagtccga
     1681 cgacggatcg tacgagggca agctgtcggt gaagttgggc ccgatggcgg cgtctttcga
     1741 aggcaaggcg gtggtcaccc cggacccggc taccaagacg gcgctgatcg aaggcaaggg
     1801 ggtcgaccgc agcggcggct ccaggggtca ggtcaaggtc aactactcga tcgtccccga
     1861 tggcgccggc tcgagagtcg gcgtggatgc cgacgtcacc ctctccggcc ccgtcgccca
     1921 gttcgggcgc acaggtctga tcaacgagat gtccaagcgc ctgatcggtg agttcgtcga
     1981 gtgtctcgag gcaaagctga ccgcggtgac cccggttgag gcggcggcga tcgaggccgg
     2041 cgaggtcaag ggcatatctc tcttcttcgc ttccctgtgg agctggctca gaggtctctt
     2101 tcgcagggga gagacctagc cttgcgtcgg atccggctcg gtctcgtctg cctcttgctg
     2161 ctggtgtcgt gcggcggaga agggggagca tcgatcactg gggacgatgt ggtggtgggc
     2221 atgttcgaca accgctatga gtacaccgag atccagatac cggtcggggg gtcggtgacc
     2281 tttcgcgggg cgggccgcaa tccgcacaac gccgtcgccg ccgacagctc gtggtcgacg
     2341 gaggacgtct ttggcagcct cgagcaactg gagggagacg aggccacgct caccttcgac
     2401 gctcccgggg agtatgtctt cttctgcacc ttccacggga acgacgaagg ggacgggatg
     2461 gccggcacgc tgaccgttgg agattccggc taatgaagag aggctgggcc ctgctggtgg
     2521 cgggggcagc cttgagtctg gcctcttgtg ccgggcctcc atttggcgac ccctcaccag
     2581 ccgccctcgg cgatcctgcg accgactcgc catcggattg gtccggcgag acccgcctcg
     2641 tcccccaggg ctatccgacg atccaggcgg cggtggacgc tgccgggccc ggtgacctga
     2701 tcctgatcga ccgcggcgtc taccgggagg aggtcgaggt gaccacaccc gggatcacca
     2761 tcagaggtgt cgaccgcaat caggtggtga tcgacggcga gttccagagg ccaaatggga
     2821 tcgaggtgtt tctggccgac ggggtggtgg tggagaacct gaccgttcgc aacgccacat
     2881 cgaacggtgt gttctggaca ggggttcggg gttatcgcgg gtcgtacctg accgcggtga
     2941 acaacggtgt ctacgggatc tatgccttcg attccagcga cggcctgctg gaccactcct
     3001 acgcctcggg atcgcccgat gccgggttct acatcgggca gtgcgacccg tgtcagtcga
     3061 ccatcaccga ttcgatctcc gagtggaacg ggctcggcta ttcgggcacc aacgcttcca
     3121 ccgacctcta catcgtcaac tcggtctttc gccacaacgt cgccggcatc gctcccaaca
     3181 ccctcgatgg tgagctgctt ccgccgtttc atggggtgaa tgtcgtgggg aacctggtgc
     3241 acgacaacgg gaacgtcgag gctccggccc tcgccttcga gtgggcggcc cagggcaacg
     3301 ggatcttgct cgcaggtgga ggtgactcct acgtgtcccg gaaccgggtg ctcaaccatc
     3361 cccagagtgg gatcgtcgtg ttgccgatgc tcgacgccaa cttctacatg tcatacgacc
     3421 accgcgtcgt ggggaatcac gtcgcgggct ccggtctcgc cgacctgacc ctgggcgggc
     3481 cggcgacgtc gggcaactgc ttcgaggaca acgtgtacga cacatcgttg ccggcggccc
     3541 tcgagttcaa gcagccgtgt gacggcattc gattcccggc cctcttcgag atcggctcga
     3601 ccacggccca gctcgggcgt ctcttcgaga gcgggctcgg gctccaaccc gaagtcgaca
     3661 tcacgtcatt gcccgtgccc gagccgcaac caccgatgcc ggacggggcc ggcgctccgg
     3721 tgctgcccg
//
LOCUS       DATITW010000073         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_243912, whole genome shotgun sequence.
ACCESSION   DATITW010000073 DATITW010000000
VERSION     DATITW010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243912"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VLS86_01010"
     CDS             complement(<1..632)
                     /locus_tag="VLS86_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO49098.1"
                     /translation="MTVPAADVEVEVPPGDGPFPAVVLVHGGGWVAGSPRLMSDLAGV
                     LTGEGYLTVNASYTLAGELPGFPVAVDDVACAVRYAAAHPDGDGTVAIIGHSAGAHLS
                     ALVALDDVGYGAGCPLEEPAIPDRLIGLAGPYDVARLGPLLLPFFGAGPREDPEAWIA
                     GNPMNHAGANTDLSSLLVHGEEDGLVDLSFATDFAEVLTEAGSEALVEVVT"
     gene            complement(791..1471)
                     /gene="pgl"
                     /locus_tag="VLS86_01015"
     CDS             complement(791..1471)
                     /gene="pgl"
                     /locus_tag="VLS86_01015"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HSO49099.1"
                     /translation="MELLVQESPEAVAEAASKRVADLISSAVSRFSLGLAGGTTPEAT
                     YRALRGRAAGWANVDAWLSDERWVPPDHERSNGRMAASTILDHVDATFHRPRVSGSML
                     PEDSAAFYEAQLRSIHPDGRPPDLVMLGLGEDGHTASLFPGTAALDERQRWYVANRVP
                     QLNEERLTATFPLLWSARLLMVLVVGVGKAGAVRASFEKTTPAGLLGEGNAEVEWYLD
                     REAASLLV"
     gene            1503..2165
                     /locus_tag="VLS86_01020"
     CDS             1503..2165
                     /locus_tag="VLS86_01020"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSO49100.1"
                     /translation="MADRITIELPDGEVSGRLAGAGPRGVLLAHGAGTNQDHPFMVSL
                     RDALAAAGHTVMTFNYRYTERGSKRPDRTESLVEVHHAAAESLRQRVDDLYLAGKSMG
                     GRMATYLAADGYPAKGLVLFGYPLHPAGKEDRLRVAHLADVQAPMLFFQATRDALSRM
                     ELFDRHVRSLPNVSVELLEGASHSFRGGGWTEETTIDRLTTGTTKWIEAVSSGRTVDS
                     RP"
     gene            2202..2873
                     /locus_tag="VLS86_01025"
     CDS             2202..2873
                     /locus_tag="VLS86_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HSO49101.1"
                     /translation="MSQVSLRAEAGRATGSRESRRLRRTGLVPAIVYGKGTDPINVAV
                     DAHDLHMALHTEAGSNALINLEIEDDKVVLTMARIIERHPFRNEYRHVDFVTVSLDET
                     VHAEVAIHFEGTPAGVIEGGVFSPQRTHVSVETLATTIPSFIELDVSAVEIGDSLRIA
                     DLPVLEGVVYTEDPEGVVMSVTTPAAEIEEPEEVEGEEGEELAEGEESDGVAAEGEAE
                     GEPTE"
     gene            2878..>3088
                     /locus_tag="VLS86_01030"
     CDS             2878..>3088
                     /locus_tag="VLS86_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49102.1"
                     /translation="MRAVVGLRNPGAEYEGTRHNSGYEVVARVLERSGESLGRAPSRV
                     RARVAQVGGGEERILYAVPLTYMNES"
BASE COUNT          516 a         1045 c         1038 g          489 t
ORIGIN      
        1 gtcaccacct ccaccagagc ttccgatccg gcttccgtga gcacctcggc gaaatcggtg
       61 gcgaagctga gatcgaccag gccgtcctcc tcgccgtgca ccaagagact cgacagatcg
      121 gtgttggccc cggcgtggtt catcgggttg ccggcgatcc acgcctcggg atcctccctc
      181 gggccggccc cgaagaaggg cagcagcagt gggcccagtc gagcgacgtc gtacggcccg
      241 gcgagaccga tcagacggtc cgggatagcc ggctcttcga gcgggcagcc cgccccgtag
      301 ccgacgtcgt cgagggcgac cagggccgag agatgggctc ccgcggaatg cccgatgatg
      361 gccaccgtgc catccccgtc cgggtgagcg gcggcgtatc tcactgcgca tgccacgtcg
      421 tcgaccgcca ccgggaaccc ggggagctcc ccggccaggg tgtaggacgc attgaccgtc
      481 agatacccct ccccggtgag gaccccggcg aggtcgctca tcagccgggg cgacccggcg
      541 acccagccgc ccccgtgaac cagcaccacc gcagggaaag ggccgtctcc aggtggcacc
      601 tccacttcga cgtcggccgc gggaacggtc acagtcgtcg tcgtcgtcgt ggtggtagta
      661 gaagtcgttg tcgttgctgg gggagcggtg gtgtccgaag tggtcgtggc cagcgccggc
      721 ggcgtcgtgc tcgatccccc tccggtcggc gcgcaggacg ccagcagcgt ggcggcaacg
      781 atcagacagg tcagacgagg agggaagccg cttctcggtc caggtaccac tcgacctccg
      841 cattgccctc gccgagcaga cctgccggcg tcgtcttctc gaagctggcc cggaccgcgc
      901 cggcctttcc caccccgacg acgaggacca tgaggagccg ggcgctccac aagagcggga
      961 aggtcgcggt gagtctctcc tcgttcaact gcggcacccg attggccaca taccagcgct
     1021 gccgctcatc gagggccgcc gttcccggaa acagcgacgc ggtgtggccg tcctcgccca
     1081 ggcccaacat caccaggtcg gggggccgcc cgtcggggtg gatcgatcga agctgcgcct
     1141 cgtagaacgc cgccgagtcc tcgggcagca tcgaaccact cactcggggc cgatgaaacg
     1201 tggcgtcgac atggtcgagg atggtggagg cggccatgcg cccgttggaa cgttcgtgat
     1261 ccggtgggac ccatctctcg tccgatagcc aagcgtcgac gtttgcccat cccgccgctc
     1321 ttccccgcag ggcccggtat gtcgcttcgg gcgtcgtccc cccggcgagg cccaaggaga
     1381 agcgggagac ggccgacgag atcagatcgg cgacacgctt ggatgccgcc tcggcgactg
     1441 cttcagggct ctcctggacg agcaactcca tccgctcacg gtaacccttc tacattgtca
     1501 tgatggccga ccggatcacg atcgagctcc ccgacggcga ggtgtcgggt cgtctggccg
     1561 gggccgggcc ccgaggggtc ctcctcgccc acggcgccgg caccaatcag gatcatccct
     1621 tcatggtcag cctgcgcgac gccctggctg cggcggggca cacggtgatg actttcaact
     1681 accggtacac cgagcgcggc tccaagcgcc ccgaccgaac cgagagtctg gtcgaggtcc
     1741 atcacgcggc cgccgagtct ctccggcagc gggtcgacga tctgtacctg gccggaaagt
     1801 cgatgggcgg ccgaatggcg acctatctgg ccgccgacgg ctatccggcg aagggactgg
     1861 tcctgttcgg ctatccgctg caccctgcgg ggaaggagga caggttgcgg gtcgcacatc
     1921 tggccgatgt gcaggcgccg atgctcttct ttcaggccac ccgggatgcc ctgagccgga
     1981 tggagctgtt cgaccgtcac gtccgctccc tgcccaatgt gtccgtggag ctgctcgagg
     2041 gcgcatccca ctcgtttcga gggggaggtt ggaccgagga gaccacgatc gaccgtctca
     2101 ccacggggac cacgaagtgg atcgaagccg tctcatctgg cagaaccgtc gattctcggc
     2161 cataatcgac ctctcaccca ctccccaagg agtctcttca gatgtcgcaa gtaagtcttc
     2221 gcgccgaggc gggccgtgcc accggttcgc gtgagtcgcg gcgtcttcgc cggacggggc
     2281 tggtgccggc catcgtctac ggaaagggca ccgacccgat caacgtcgcg gtggacgccc
     2341 acgacctgca catggcgctg cacaccgagg ccggctccaa cgcattgatc aacctcgaga
     2401 tcgaggacga caaggtggtg ctcaccatgg cgcggatcat cgagcgccat ccatttcgca
     2461 acgagtaccg gcacgtcgac ttcgtcaccg tctctctcga cgagacagtc cacgccgagg
     2521 tcgcgatcca cttcgagggg acgccggcgg gtgtcatcga aggtggggtc ttctcaccac
     2581 agcggactca cgtgagtgtc gagaccctgg ccaccaccat cccgtcgttc atcgagttgg
     2641 acgtctccgc tgtcgagatc ggcgattcgc ttcggatcgc agatctgccc gtcctcgaag
     2701 gtgtcgtcta caccgaggac cccgagggcg tcgtgatgag cgtcaccacc ccggccgccg
     2761 agatcgaaga gccggaagaa gtcgaaggcg aagagggcga ggagctcgct gagggtgagg
     2821 agtccgatgg cgtggcggct gagggcgaag ccgagggcga gccgaccgaa tagcccgatg
     2881 cgtgccgtcg tcggtctccg caacccgggg gccgagtacg aggggacgcg ccacaactcc
     2941 gggtatgagg tggttgcccg cgtcctggag cgctcagggg agagtctggg ccgggctccg
     3001 tcccgggtca gggcccgggt ggcccaggtc ggtggtggcg aagagcggat cctctacgcc
     3061 gtccctctca cctacatgaa cgagtcgg
//
LOCUS       DATITW010000074         1734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_764812, whole genome shotgun sequence.
ACCESSION   DATITW010000074 DATITW010000000
VERSION     DATITW010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764812"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VLS86_01035"
     CDS             <1..461
                     /locus_tag="VLS86_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49103.1"
                     /translation="SKQYLVFLAPLLLLHRDEGWGKRAATATATALVVLVPPLLLDPV
                     AYYDAIIGNPTGVGFRPDSVSLMGLLDGFGIRFEPAHWVWLVYGLAVAVIVSIGSRVW
                     STFLGRAGLILGLTFFMSVAFANYWFLIMGMVAISTILDDRDRTTVMASV"
     gene            549..>1734
                     /locus_tag="VLS86_01040"
     CDS             549..>1734
                     /locus_tag="VLS86_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HSO49104.1"
                     /translation="MSTDSILIRGAREHNLKNLTVELPRNKLIVFTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAISIDQKTSSRNPRSTVGT
                     VTEVHDYLRLLYARIGIAHCPNDGTPVERQTPQQIVDRILELEEGTRFQVLAPVVRGR
                     KGEYEGLLSDLAKEGFARARIDGEVRELTEEIKLDRYYQHTIEVVVDRLVRKEGMEQR
                     LTQSLETALTLTEGTADIEIEDGATLTFSQHLACSLCGLSFEDLQPRNFSFNSPYGAC
                     PVCSGIGTKFQVDPELVIADPGRSLEDGAIPMWSSNRMRYYHRLLSGLSEVFGFSMTT
                     PWQDLSPEIKNKILYGTGEDKITIKYTNRFGRNRQWQATFEGAIPFLNRRHEAAESDS
                     ARE"
BASE COUNT          317 a          557 c          532 g          328 t
ORIGIN      
        1 catcgaagca atatctggtg tttctcgccc cgttgctgct cctccaccgc gacgagggtt
       61 ggggaaagcg ggcggctacg gcgacggcga ccgccctggt cgtgctcgtg ccaccgctat
      121 tgctcgatcc ggtggcctac tacgacgcga tcatcgggaa tccgaccggg gtcgggttcc
      181 gacccgacag tgtgagcctg atggggctgc tcgatgggtt cgggatcagg ttcgagccgg
      241 cgcattgggt ctggctggtc tatgggctcg cggttgcggt catcgtcagc atcggctcga
      301 gggtgtggtc caccttcctg gggcgagccg gcctcatcct cgggctcacc ttcttcatga
      361 gcgtcgcctt tgccaactac tggttcctga tcatggggat ggtggccatc agcaccatcc
      421 tcgacgatcg cgaccggacc actgtcatgg catcggtgtg acgtctcggc ggtctctgac
      481 cacgaacaag tgttcgcctc gaatctggat tacactgtcg taactcaggc gaccaagcag
      541 gcggagcgtt gtccaccgat tcgatactca tcaggggcgc ccgtgagcac aatctgaaga
      601 acctcacggt cgaactgccc cgtaacaagc tgatcgtctt caccggtctc tccggatcgg
      661 ggaagtcctc ccttgccttc gacaccatct atgccgaggg gcaacggcgc tatgtggagt
      721 ccctgtctgc atacgcccgt cagttcctgg ggcagatgga caagccagac gtcgatttca
      781 tcgaagggct gtcacccgcg atctcgatcg accagaagac gtcttcccga aacccgaggt
      841 cgacggtggg caccgtcacc gaggtccacg attatctcag gcttctctat gccaggatcg
      901 gcatcgccca ttgtcccaac gacgggacac cggtcgagcg ccagacgccg cagcaaatcg
      961 tcgaccgcat cctcgagttg gaggagggca cccgattcca ggtgctggca cccgtggtcc
     1021 gggggcgcaa aggggagtac gaggggctgc tctccgacct ggccaaggag ggcttcgccc
     1081 gggcccgcat cgatggcgaa gtgcgagagc tcaccgagga gatcaagctc gatcgctact
     1141 accagcacac catcgaggtg gtggtcgatc gtctggtgcg caaggaaggg atggagcagc
     1201 gtctaaccca gtcgttggag acggcgctca ccctcaccga gggcaccgcc gacatcgaga
     1261 tcgaagacgg agccaccctc accttctccc agcatctggc ctgctcattg tgcggcctct
     1321 ccttcgagga cctccagccc cgcaacttct cgttcaacag cccttacggc gcctgcccgg
     1381 tgtgctcggg gatcgggacc aagttccagg tcgaccccga gttggtcatc gccgaccccg
     1441 ggcgttctct cgaggacggg gcgatcccca tgtggtcctc gaaccggatg cgctactacc
     1501 accggcttct gagcgggctc agcgaggtgt ttggcttctc gatgaccacg ccctggcagg
     1561 acctctcacc ggagatcaag aacaagatcc tctacggcac cggtgaggac aagatcacga
     1621 tcaagtacac caaccggttc gggcgcaatc ggcaatggca ggcgaccttc gagggcgcca
     1681 tcccgtttct caatcggaga cacgaggcgg ccgagtcgga ctccgcccgg gagt
//
LOCUS       DATITW010000075         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_444363, whole genome shotgun sequence.
ACCESSION   DATITW010000075 DATITW010000000
VERSION     DATITW010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444363"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VLS86_01045"
     CDS             <1..366
                     /locus_tag="VLS86_01045"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HSO49105.1"
                     /translation="LVPLLVGLGGSWSLLFYNNGKEALVSPITDTEITAIFETGRLRG
                     HSGCNRYTTTYEADGGSLRIPPPVGTRMLCSEPPGLMEQEARYLELLPTTDSYLLRDG
                     RILELFDPAGARILQFSRT"
     gene            complement(404..1465)
                     /locus_tag="VLS86_01050"
     CDS             complement(404..1465)
                     /locus_tag="VLS86_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HSO49106.1"
                     /translation="MTTETLALVIAAASVLGFVGLAVVIIVTRSSAGPVDPPMSAPEM
                     ARRLEAVQSALANRDGAFASQVDQLDTKLAMLQQAVTGREAALDEQVRGIGSQMQSIT
                     SLFTNDRARGGWAEIGMRRIFEQGGLVEGRDFTCQVNGGDVHPDAVVYLPGGRNIVID
                     AKFPVARFVEALTEEDPDRRRRLLGEQGRELERVGKSLTAKGYAELASGGYVVMYLPS
                     QAVYEAAASAHPELLDRLMTARVIIAGPSTLYALLLNVASLLTEFKALQQADQILDDA
                     RELHKRMATFAGHLQGVGVGLTGAVRAFNGAVGSWGSRVAPQLTRMSDQTGRAEIDAP
                     AVVEEAVREVPEPALRVVG"
     gene            1554..2066
                     /locus_tag="VLS86_01055"
     CDS             1554..2066
                     /locus_tag="VLS86_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HSO49107.1"
                     /translation="MIHFDNPFRPDPGDRDQARRFRGRLAAGVTIVTSGAGPDRTGLT
                     VSSLLVIEGSPALVHLLVGPSTDLWAVAAESGHFVVHACAYDHHQLADVFAGLSPSPG
                     GLFAPVEATPSDWGPVLTDLGDRLYCSFVSKEEMGWSGIVIGRIDEIEVSDLDDPLIH
                     FRSSYRRLGK"
BASE COUNT          344 a          722 c          645 g          382 t
ORIGIN      
        1 ctggtcccgc ttctggtcgg gctcggggga tcgtggtctc tgctcttcta caacaacggc
       61 aaggaggccc tcgtcagccc gatcaccgac acggagatca ccgcgatatt cgagacagga
      121 agactgaggg gtcacagcgg ctgcaaccgg tacacgacga cctacgaggc ggacggagga
      181 tcgctccgca tccctccacc ggttgggacc cggatgctgt gctcggagcc accgggactc
      241 atggagcagg aagcgaggta cctcgagctg ttgcccacca cggacagcta tctgctgcga
      301 gacggacgga tcctcgagct gttcgacccc gccggggccc ggatcctcca gttctccagg
      361 acttgaacga tcgatctcgg atgcgccacg gcgggccagg tgatcacccg accacccgca
      421 gggccggttc ggggacttcc cggaccgcct cctcgaccac cgccggtgcg tcgatctcgg
      481 cccgtcccgt ctggtcgctc atcctggtga gctgtggtgc cactctgctc ccccaggatc
      541 cgacggcgcc attgaaggcc cgcaccgcac cggtcaaccc gacgccgact ccctggagat
      601 gaccggcgaa cgtagccatc cgcttgtgga gctcacgggc gtcgtccagg atctgatccg
      661 cctgctgcag ggccttgaac tcggtgagca gcgaggcgac gttcaacagc aaggcgtaaa
      721 gggtgctcgg cccggcgatg atcacccgcg ccgtcatcaa ccggtcgagg agctcgggat
      781 gagccgaggc cgccgcctcg tatacggcct gcgaaggcag gtacatcacc acgtagccac
      841 ccgaagccag ctcggcatag cccttggcgg tgaggctctt tcccacccgc tccagctccc
      901 tgccctgttc tccgagcagg cgacgacgcc gatcagggtc ctcctcggtc agggcctcga
      961 cgaatctcgc caccgggaac ttggcgtcga tcacgatgtt ccgcccaccc gggaggtaga
     1021 cgacggcatc ggggtggaca tcgccgccgt tcacctggca ggtgaagtca cgaccctcga
     1081 ccagtccccc ttgttcgaag atccgcctca tcccgatctc cgcccagccc cctcgggccc
     1141 ggtcgttggt gaacaacgac gtgatcgact gcatctgcga cccgatgcca cgcacctgct
     1201 cgtccagagc cgcttcgcga ccggtcaccg cctgctgaag catggcgagc ttggtgtcca
     1261 actggtcgac ttgcgaggcg aacgccccat ctcgattggc gagggccgat tgcaccgcct
     1321 cgagacgtcg tgccatctcg ggggccgaca tcggggggtc gaccgggccc gcgctgcttc
     1381 tggtcacgat gatgacgacg gcgaggccga cgaagcccag cacagaagcc gctgcgatca
     1441 ccagggcgag tgtctcggtt gtcatgttct catcctcgtt tttcggtccc gcttctactc
     1501 ctgacctcat cctctcatgg gggtgtgaca gatttgccta gggtcaccgt ccgatgatcc
     1561 atttcgacaa ccctttccga ccagatcccg gggaccgcga tcaggcccga cgcttccgtg
     1621 ggcggctcgc ggctggagtc accatcgtga ccagcggggc aggcccagat cgaaccgggc
     1681 tcactgtctc ctcacttctc gtgatcgaag gcagtccggc tctggttcat ctcttggttg
     1741 gccccagcac cgacctctgg gcggttgcgg ccgagagcgg gcacttcgtg gttcacgcct
     1801 gcgcctatga ccaccatcag ctggctgatg tgttcgcagg cctgtccccc tcccccggtg
     1861 gtctgttcgc accggtcgaa gcgacaccct cggactgggg cccggtgttg accgatctcg
     1921 gcgaccgcct ctattgcagc ttcgtgtcga aggaagagat gggatggtcg ggaatcgtga
     1981 tcggccggat cgacgagatc gaggtgtcgg atttggacga tccgttgatc cacttccgca
     2041 gcagctaccg ccgtctcggc aaatgacctg ctccgtggcc gccgcttcgc ggc
//
LOCUS       DATITW010000076         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_3678, whole genome shotgun sequence.
ACCESSION   DATITW010000076 DATITW010000000
VERSION     DATITW010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3678"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..511)
                     /locus_tag="VLS86_01060"
     CDS             complement(14..511)
                     /locus_tag="VLS86_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSO49108.1"
                     /translation="MARVLVLNASYEPLSVVTARRAVVLVLNQKASIVVGRDEVWSSE
                     SYLVDVPSVVRLNRFVKVPYRRAAPVTRRAVFGRDGHRCQYCGGPAESLDHVLPRSKG
                     GDHTWENVVACCRPCNIHKGDRLPVDAGLRLNTNPGPPRRYAWIYASTGYQVDPAWHP
                     YLGAA"
     gene            complement(555..1109)
                     /locus_tag="VLS86_01065"
     CDS             complement(555..1109)
                     /locus_tag="VLS86_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49109.1"
                     /translation="MRERLEDIPGVASVSLDLEDVESGIKVKLDDDANEGEVLERVRE
                     LLVAYGVRSHDHPTLQIGQSRFGRVGVDIGVAVEVTPVKGGARVEVIGPTVRSFRVVP
                     PNPTAIAQGMSDAWCQVLGKIPVEITRVSRTGDESLRVVATDGSTERDGLGDLAYGWI
                     YALAQAVGKAIGVLDAESGAKMRN"
     gene            1423..2424
                     /locus_tag="VLS86_01070"
     CDS             1423..2424
                     /locus_tag="VLS86_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSO49110.1"
                     /translation="MNHPGAHVTEISPAQIELFSERFGRIAANVEKVIQGKREVIELV
                     LLALVAEGHVLVEDVPGVGKTQLAKSVARSIDSLYQRIQFTPDLLPSDVTGVSVWDRN
                     RNEFEFKPGPIFANIVVGDEINRASPKTQSALLEAMEERQVTSDGVTRALPLPFLVMA
                     TQNPLEHEGTYPLPESQLDRFMMRVVIGYPSRQKELEILDTHGVRSTFFDLEPVVSVE
                     EVEEMVAIARSVAVSQSVKSYIVDLVEGTRSHSDILLGASPRSALFLQRLSRARAAAA
                     GREYVTPDDIKFLAHPVLEHRMGLRPEAQMRGESLPEAIDELMTRLRVPGTTTRIAT"
     gene            2424..>3341
                     /locus_tag="VLS86_01075"
     CDS             2424..>3341
                     /locus_tag="VLS86_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSO49111.1"
                     /translation="MPTLRGWALSGAGLALILLWYALGETELLLTGLFLLVAQLVAVV
                     YVRTRRPNLDINRRLGSSTVHDGETTTITLLVENLSRRPIGYLTVDDDVNRLGVATFE
                     VARLKKKESTTATYRVTCRPRGVYRVGPTTVTATDPLGLAETSAGIGPVDRIVVYPAV
                     EELSGFPMSRGQDPAMQASRPDHSTRGGEDFYTLREYQRGDDLRRVHWPSSARTDVLM
                     IRQLETPWQSRALVLLDVRSGSYESQDAFEKAVSGAATIVTHLVGAGFDADLWAGDAE
                     PIDASRYTTAMERLALVQPDPAIDIEAVAA"
BASE COUNT          590 a         1139 c         1029 g          583 t
ORIGIN      
        1 gtcatcgcgg tgcttatgca gccccgaggt aggggtgcca ggcgggatcg acctggtagc
       61 cggtggaggc atagatccag gcgtagcggc gtggcggccc ggggttggtg ttgagccgga
      121 gccctgcgtc cacggggaga cggtcaccct tgtggatgtt gcaggggcgg cagcaggcaa
      181 ccacgttctc ccatgtgtga tcgccgccct tggagcgggg aagcacgtgg tcgagggact
      241 cggccggccc tccgcagtat tggcagcgat gcccgtcccg accgaacacg gcacgacggg
      301 tgaccggagc cgcccgacgg tacgggacct tcacgaagcg attgagccgc accaccgaag
      361 ggacgtcgac cagatagctc tccgagctcc acacctcgtc tctccccacg acgatgcttg
      421 ccttctggtt gagcacgagg acgaccgccc tgcgcgcggt caccaccgag agcggttcat
      481 agctggcatt tagtacgaga accctggcca taacgtgcgc ttgtcgctga cgcgacgtta
      541 ccaagcagat caggtcaatt tcgcatcttt gcgccggatt cggcgtcgag aaccccgatc
      601 gccttcccca cggcctgggc cagggcatag atccagccat aggccaggtc gccgaggcca
      661 tcgcgctcgg tgctgccgtc ggtggccaca acccggagcg actcgtcacc ggtgcgggag
      721 acgcgggtga tctccacggg gatcttcccc agtacctggc accaagcgtc cgacatgccc
      781 tgtgcgatcg cggtcgggtt cggtggcacc acccggaacg atcgcaccgt gggaccgatg
      841 acttcgaccc gggctccccc cttcaccggt gtcacttcga ctgcgacccc gatatcgacg
      901 ccgactcgcc caaaccggga ttgcccgatc tggagcgtgg gatgatcgtg ggaacggact
      961 ccgtaggcga ccaggagctc acgtacccgc tccaggacct ctccctcgtt ggcatcgtcg
     1021 tcgagcttga ccttgatccc cgattccacg tcctcgaggt cgagcgagac cgaggccaca
     1081 cctggtatgt cctcgagacg ttctctcaat ttcgctctca cgaaaggcct cgcattcgac
     1141 gtcgccacag cagataagac tccaaatcct gtcgttgggt ttccgggttg tcgaccccgt
     1201 atgccgtatg aaccctccag tcgatgtacg tcgacgatgg gaacacgcct cgacgggacc
     1261 gcatcgcagc accggcccgt atcgcctccc agagcaacac cggccgacga acaagcgtca
     1321 gcagcatccc ggccatcccc agcccatcac tcatcagata gccactccct gggtgacagt
     1381 ctagctccca cagcgcctgg tcatcggtag agtttcgtgc caatgaacca cccgggagcg
     1441 catgtgaccg agatttcgcc agctcagatc gagctgttca gtgagcggtt tggccggatt
     1501 gccgccaatg tcgagaaggt gatccagggc aagcgggaag tcatcgagct ggtgttgctc
     1561 gcgttggtcg ccgaagggca tgtgttggtc gaggacgtcc ccggcgtggg caagacccaa
     1621 ctggccaaat cggttgcccg atccatcgac agcctgtacc agaggatcca gttcaccccc
     1681 gatctgctcc cctcggatgt gaccggtgtc tcggtctggg ataggaaccg aaacgagttc
     1741 gagttcaagc cagggccgat cttcgccaac atcgtcgtcg gcgacgagat caaccgtgcc
     1801 agtcccaaga cccagtccgc tcttctcgag gccatggagg agcgccaggt gacctcggac
     1861 ggtgtcaccc gcgctctccc cctccccttc ctggtcatgg ccacccagaa ccctctggag
     1921 cacgagggca cctatccgct tcccgaatct caactggatc gattcatgat gagggtggtc
     1981 atcggctacc cgagccgtca gaaagagctg gagatcctgg acacccatgg tgtccgctcc
     2041 accttcttcg atctggagcc ggtcgtctcc gtcgaggagg tggaggagat ggtcgccatc
     2101 gcccgctcgg tggcagtgtc ccagtccgtc aagtcctaca tcgtcgacct ggtcgagggc
     2161 actcgaagcc actcagacat cctcctcggt gcctcccccc gatccgctct ctttctccag
     2221 cgtctgtctc gggcccgggc tgctgccgcc ggccgcgagt acgtgactcc cgatgacatc
     2281 aagttcctcg cccaccctgt gctcgagcac cgaatggggc tcagacccga ggcccagatg
     2341 cgcggcgagt cactgcccga ggccatcgac gagctgatga ccaggctccg tgtcccgggc
     2401 accaccaccc ggatcgccac ctgatgccaa cgctgcgagg ctgggcgctg tcgggagccg
     2461 gcctcgccct gatcctcctc tggtatgccc tgggtgagac cgagcttctg ctcaccgggc
     2521 tgttcctgct cgtggcgcaa ctggtggccg tcgtctacgt gcgcaccagg agacctaacc
     2581 tcgacatcaa tcgacgcctg ggctcgtcga ccgtgcacga cggcgagacc accaccatca
     2641 cactcctcgt cgagaacctc tcacggcgcc cgatcggcta tctgaccgtc gacgacgacg
     2701 tcaatcgtct cggggtggcg actttcgagg tggcaagact caagaagaag gagtcgacca
     2761 ccgccaccta ccgggtcacc tgccgtccac gcggcgtcta tcgcgtcggg ccgaccaccg
     2821 tgactgccac cgatccgctc gggctcgccg agaccagcgc cggcatcggg ccggtggaca
     2881 ggatcgtggt ctaccccgcg gtggaagagc tcagcgggtt cccgatgagc cgcgggcagg
     2941 acccggcgat gcaagcctcg aggccggacc acagcacgcg tggcggcgag gacttctaca
     3001 cgctcaggga ataccagcgg ggcgacgacc tgcggcgggt gcactggccc tcatcggccc
     3061 ggaccgatgt gctgatgatc cgccagctgg agacgccgtg gcagtcgcgg gccctcgtcc
     3121 tgctcgatgt ccgcagtggc agctacgagt ctcaggacgc gttcgagaag gcggtgtcgg
     3181 gtgcagccac catcgtgacc catctcgtcg gcgccggttt cgacgccgac ctctgggcag
     3241 gagacgcgga gccgatcgac gcctctcgct acacgactgc catggagcgg ttggcactgg
     3301 tccagcccga cccggcaatc gacatcgagg cagtggcggc t
//
LOCUS       DATITW010000077         1866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_724729, whole genome shotgun sequence.
ACCESSION   DATITW010000077 DATITW010000000
VERSION     DATITW010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724729"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /gene="glmS"
                     /locus_tag="VLS86_01080"
     CDS             <1..1237
                     /gene="glmS"
                     /locus_tag="VLS86_01080"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HSO49112.1"
                     /translation="LEFTRQMVVFEDDRVVEIRPDGVTLRALDGNILPLDERRVEWDL
                     EAAEKGGFPTFMLKEIFEQPHAIADTLAGRVEEPGGVVLDELTIEPEVLREIDKVFVV
                     ACGTSYHAAMMAKYAIERWARLPVEIDIASEFRYRDPVLDARSLVIGISQSGETIDTL
                     AAIRYARAQGSLVIGVSNVVESALARESDAVLYTRAGPEIGVAATKTFTTQLVAMQLL
                     GLYLAQVRGALDPGAIEVQVKELLRLPDQVESVLARAGEVEALSGLWSETRDWFFLGR
                     SGDFPVAMEGALKLKEIAYVRAEGYAAGEMKHGPIALIEPGVVVVGVATDSHVQAKTL
                     SNMEEMKARGGTILLIAEEGDDVGDVADHVIRVPAGPDLLCTVTAVVPLQLLAYYVAT
                     AKGMDVDKPRNLAKTVTVE"
     gene            complement(1307..>1866)
                     /locus_tag="VLS86_01085"
     CDS             complement(1307..>1866)
                     /locus_tag="VLS86_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HSO49113.1"
                     /translation="RTTVAAQAPTAGLWQIDQSHSDLRITARHLMVAKVRGTFADFAG
                     TIVVADDPTESTVEFEAKAASITTGTADRDAHLRSPDFLDAEQHPLITFTSTALATAG
                     DRWKLSGDLTIRGISKPVTFDMAFEGEMTDPYGNTKAAFNAVGEIERKDWGLTWNVPL
                     EGGGVLVSEKLKLEFDVEAGLQTSS"
BASE COUNT          304 a          579 c          654 g          329 t
ORIGIN      
        1 cctcgagttc acccggcaga tggtggtctt cgaggacgat cgagtggtcg agatccggcc
       61 cgacggggtc acgcttcgcg ctctcgacgg gaacatcctc cccctcgacg agagacgcgt
      121 ggagtgggac ctggaggcag cggagaaggg cgggttcccg accttcatgc tgaaggagat
      181 attcgaacag ccccatgcca tcgccgacac cctcgcgggt cgggtcgagg agccgggagg
      241 agtggttctc gacgaattga ccatcgagcc cgaggtgctt cgagagatcg acaaggtctt
      301 cgtcgtcgcc tgcggcacgt cataccacgc ggcgatgatg gccaagtacg cgatcgagag
      361 atgggcgcgt ctcccggtgg agatcgacat cgcctccgag ttccgctacc gggatccggt
      421 tctcgacgcc cgctccctgg tgatcgggat cagccagtcg ggggagacga tcgacactct
      481 ggccgcgatt cgatacgcaa gagcgcaggg ctcgctggtc atcggtgtct ccaatgtcgt
      541 cgaatccgct ctggcccggg agtcggatgc ggtcctctac acccgcgccg gccctgagat
      601 cggggtagcg gccaccaaga ccttcaccac ccagctggtg gcgatgcagc ttctcggcct
      661 gtatctcgcc caggtacggg gggctctcga ccccggtgcg atcgaggtcc aggtcaagga
      721 gttgctcaga ctgcccgacc aggtggagtc ggtgctggcc cgcgccgggg aggtggaggc
      781 cctgtccggt ctgtggtcgg agacccgcga ctggttcttt ctcggcagat ccggtgactt
      841 cccggtggca atggaggggg cgctcaagtt gaaggagatc gcctacgtcc gcgccgaggg
      901 ctatgccgca ggcgagatga agcatggccc gatcgctctc atcgagccgg gtgtcgtcgt
      961 ggtcggggtc gccaccgaca gccacgtcca ggccaagacc ctgtccaaca tggaggagat
     1021 gaaggcgcgg ggcggcacca tcctgctcat cgccgaggag ggggacgatg tgggcgatgt
     1081 cgccgaccat gtgatcaggg tccccgccgg gcccgatctc ctgtgcaccg tgaccgcggt
     1141 cgtgccactt cagttgctcg cctactacgt ggccacggcg aaggggatgg atgtcgacaa
     1201 gccgcgcaac ctggccaaga cggtgacagt ggagtgacga gaaaggagcc cggcgtgccg
     1261 ggctcctttc ccggggacgt tggccggccc cgccgtcgta tcgggatcag ctcgaagtct
     1321 ggagcccggc ctcgacgtcg aactcgagct tgagcttctc ggacacgagg acgccgccac
     1381 cctccagtgg gacgttccag gtgaggcccc aatccttgcg ctcgatctcg cccaccgcgt
     1441 tgaaggctgc cttggtgttc ccgtaggggt cggtcatctc gccttcgaag gccatgtcga
     1501 aagtcaccgg cttgctgatc ccgcggatcg tgaggtcacc gctgagcttc cagcggtcgc
     1561 cggccgtggc cagggcggtc gaagtgaagg tgatcagcgg gtgctgctcc gcgtcgagga
     1621 agtccggcga gcgaagatgg gcatctctgt ccgcggtgcc agtcgtgatc gaggccgcct
     1681 tggcctcgaa ttcgacggtc gactcggtcg gatcgtcggc gaccacgatg gtccctgcga
     1741 agtcggcgaa cgtcccgcgc accttggcca ccatgaggtg acgggcggtg atgcggaggt
     1801 cactgtgtga ctggtcgatc tgccacaacc cggcggtcgg ggcctgtgcg gcaacggtgg
     1861 tgcgtg
//
LOCUS       DATITW010000078         2304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_244067, whole genome shotgun sequence.
ACCESSION   DATITW010000078 DATITW010000000
VERSION     DATITW010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244067"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VLS86_01090"
     CDS             complement(<1..640)
                     /locus_tag="VLS86_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49114.1"
                     /translation="MTTTTVDKAPATEKRPGGWRVVAAKEVADHLLSGRFVVLMGVLG
                     IATAAAVFAASGGLRDLGAETEGVTALFLKLFTVAADPVPRSLLFFVAFLGPLLGIMF
                     GFDAVGGERSQGTLPRLLSQPIHRDEVILGKFVAGLAVVTIMLVSLVLFVSGIGIARL
                     GVVPSPAEISRLVVWLVFTIIYIGLWQALATLCSVVAKRAATAVMIPVGIWLV"
     gene            complement(637..1581)
                     /locus_tag="VLS86_01095"
     CDS             complement(637..1581)
                     /locus_tag="VLS86_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008504885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO49115.1"
                     /translation="MTTNIRGKGLTKTYGEVTVVDELDLEIGAGEVFGLLGPNGAGKT
                     TTILMLLGLVEPTSGSMEVLGLDPRRNPLEVKRRVGYLPDAVGFYDSMTGRENLRFTA
                     RLNGLEDFEGLIQSLLETVGLEEAADQPAGEYSRGMKQRLGVADALVKDPSVLILDEP
                     TTAIDPEGVAEMLDLIRRLAKEDGVTVLLASHLLHQVQTVCDRVAIFVRGKIVAQGLP
                     HELAAVEKGGRITVEFRMDGDPAEGRRHLESLGTVSAVTPGQIPGSWVGELEAQSLAP
                     TVSGLVSGGANVIGVHRRDEDLESTYRRYFETHEEVPA"
     gene            complement(1583..>2304)
                     /locus_tag="VLS86_01100"
     CDS             complement(1583..>2304)
                     /locus_tag="VLS86_01100"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HSO49116.1"
                     /translation="PNFPGLRTPAGQAVTFDVELANGTPADLEFELAPSGPAGWDVTA
                     ELAGQAQASTVQVEAGGTETINVEVTPPFQAEAAQYPISLRVTSAETQVETEMIVEVV
                     GSFAIDLTTPDQRLNAEVTVGSSSEFQLLLTNSGTAPLEEVIVGVTPPSGWEVTFDNQ
                     TVSIPPGETAAVTATITPSEEAVAGDYVITFTATNEQVDDQIEVRTTVNPSPVWGFVG
                     IAVIALTLAGLAWVFRRFGRR"
BASE COUNT          396 a          788 c          759 g          361 t
ORIGIN      
        1 gcaccagcca gatgccgacg gggatcatga ccgctgtcgc agcccgcttc gccaccaccg
       61 agcacagggt ggccaaagcc tgccacagcc cgatgtagat gatggtgaag accagccaga
      121 cgacgagccg ggagatctcg gccggcgaag ggaccacccc gagccgggcg atcccgatcc
      181 ccgacacgaa cagcaccaag gagacgagca tgatcgtcac cactgcgaga ccggcgacga
      241 acttgcccaa gatcacctcg tcgcggtgga tgggctggga caggagccgg ggcagcgttc
      301 cctgggagcg ctcgccgccg accgcgtcga acccgaacat gatcccgagc agcgggccca
      361 ggaacgcgac gaagaagagc aggctccgcg gaaccgggtc ggcggcgacc gtgaacagct
      421 tgaggaacag ggcggtgaca ccctcggtct cggcccccag atcgcgcagg ccgcccgagg
      481 cggcgaacac ggccgcggcg gtggcgatgc cgagcactcc catcaggacc acgaaacgcc
      541 cggacaggag atggtcagcc acttccttgg cggctaccac ccgccatccg ccaggtcgct
      601 tctcggtggc cggcgccttg tcgacagtgg ttgttgtcat gccggcacct cctcatgggt
      661 ctcgaagtag cgtcggtatg tcgactccag gtcctcgtca cgacgatgga ccccgatcac
      721 gttggcgcct cccgacacca gcccgctgac ggtgggagcg agggattgcg cctccagctc
      781 gccgacccac gaccccggga tctggcccgg ggtgaccgcg gagaccgttc ccaacgactc
      841 gagatgtctt cggccttcgg cgggatcgcc gtccatgcgg aactcgaccg tgatccgccc
      901 ccctttctcc acggcggcca gctcgtgggg cagaccctgg gcgacgatct tccctcgaac
      961 gaagatcgcc acccggtcgc acacggtctg gacctgatgc aggagatggc tggcgaggag
     1021 gacggtcacc ccgtcctcct tggccagtcg acggatcaga tccagcatct cggcaacgcc
     1081 ttcgggatcg atggcagtgg tcggctcgtc gaggatgagc acagacgggt ccttgaccag
     1141 ggcgtcggcc accccgagcc tttgtttcat gcccctcgag tactccccgg cgggttgatc
     1201 ggctgcctct tccaagccca cggtctcgag cagggactgg atgagcccct cgaagtcctc
     1261 aagcccgttg aggcgggcgg tgaacctgag attctcccga ccggtcatgg agtcgtagaa
     1321 gccgaccgcg tccggtagat aacccacgcg acgcttcact tcgagcgggt tgcgccgcgg
     1381 gtcgagcccc agcacctcca tcgacccact ggtcggctcc accagaccga gcagcatgag
     1441 gatcgtcgtc gtcttccccg cgccgttggg cccgagcaga ccgaacacct cgccggcgcc
     1501 gatctcgagg tcgagctcgt cgaccaccgt gacctcgccg taggtcttgg tcagaccctt
     1561 gcctcgaatg ttggtggtca tctcagcgtc gtccgaagcg cctgaacacc caggcgagac
     1621 cggccagcgt cagcgcgatc accgcgatcc cgacgaagcc ccagaccggc gacgggttga
     1681 ccgtggtccg cacctcgatc tggtcgtcga cctgctcgtt ggtcgcggtg aaggtgatca
     1741 catagtcccc ggctactgct tcctccgaag gcgtgatcgt tgcggtcacc gcggccgtct
     1801 ccccgggggg gatggacacg gtctggttgt cgaaggtcac ctcccagcca ctgggtggtg
     1861 tgaccccgac gatgacctcc tccaggggag cggtgcccga gttggtgagc agcagctgga
     1921 actcgctcga gctgccgacc gtcacctcgg cgttgagcct ttggtcggga gtggtcagat
     1981 cgatggcaaa cgagccgacg acctcgacga tcatctcggt ctccacctgg gtctcggccg
     2041 aggtgacacg aagactgatc ggatattgcg cagcctcggc ctggaacggc ggggtcactt
     2101 cgacgttgat ggtctccgtc cccccggcct cgacctggac ggtcgaagcc tgcgcctggc
     2161 cggccagctc cgccgtgacg tcccagccgg cggggccgga aggagccagc tcgaactcga
     2221 ggtcggccgg ggtgccgttg gcgagctcga cgtcgaaggt gacggcctgc ccggcgggtg
     2281 tacgcagacc ggggaagttg gggg
//
LOCUS       DATITW010000079         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_164148, whole genome shotgun sequence.
ACCESSION   DATITW010000079 DATITW010000000
VERSION     DATITW010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164148"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1730
                     /locus_tag="VLS86_01105"
     CDS             <1..1730
                     /locus_tag="VLS86_01105"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HSO49117.1"
                     /translation="LMAISRREPGLYFSAQAQVHHDRLSTFLEHAMRCLDLSEVSPTL
                     SLKVSVESMVLLKEVLDRIDLPEPASVPDTQAMAAASLDRWRIPHTEIVIAKVAKGDR
                     AGDFLFSPATLQRLPEFYEKVRDRPYKPGSWEGVHTFFTTMGVARYVPWKLTERLPPW
                     AKALVWEQALWRWIVFGSALLTVLGAVTIVRRWINRRGDVHPIRQDVRRLVLPVSVSL
                     AAWFLGFVSDAINITGQARDVIATGLGAILFVALAWSVLMIGELIAEGIVASPRFRTR
                     SLDEAIVRLTSRILALISAVLVTVYGAQSLGLPVVPLLAGLGVGGLALALAAQPTIEN
                     LIAALTLHADRPVRVGDFCRFGDALGIVEEIGMRSTRIRTLDHTVLSVSNADFSKGRV
                     ENYSARRKMWYHPRIRLRYETTPDQVRYILVEIRKLLYAHPRVLPDPARIRFVGFGEY
                     SLDLDVFAYVDTKDYGEYLEIAEDLNLRIMDVVKAAGTELAIPARLEYQMDKAPLDEK
                     GIQQAEAHVEEWRGQQAMYLPKFPPETIANLRGSLDYPPAGSPGMTGERQERDPSSDT
                     PRPASGRRS"
     gene            complement(1746..>2096)
                     /locus_tag="VLS86_01110"
     CDS             complement(1746..>2096)
                     /locus_tag="VLS86_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008851103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO49118.1"
                     /translation="VTVVQDVNDVIRSNPDALSIEADDVPTAVDNMRPGHRFIRALTE
                     RPVAPGVRTHSIIAVVGSGPVTGKTDGVVAYESAHLDGVASEKVVRSGHSTQANPETI
                     LEVRRILREHVAER"
BASE COUNT          343 a          655 c          712 g          386 t
ORIGIN      
        1 ccctgatggc gatctctcgg cgggagccag gactctactt ttctgcgcag gcacaagtcc
       61 accacgatcg tctcagcacc ttcctggaac acgccatgcg ttgtctcgat ctcagcgagg
      121 tgtcgcccac gctgagcctg aaagtcagtg tggagtccat ggttctcctc aaggaggtcc
      181 tcgaccgtat cgacctgccg gaaccggcat cggtacccga cacccaggcc atggcggcgg
      241 cgagtctcga tcggtggcgg attccgcaca cggagatcgt catcgcgaag gtggccaaag
      301 gggaccgggc gggtgacttt ctcttctcgc cggcgacgct ccagcgcctc ccggaattct
      361 atgaaaaggt gcgggaccga ccgtacaagc ccgggtcctg ggaaggcgtg cacacctttt
      421 tcaccacgat gggggtggcc cggtacgttc cctggaagct gaccgaacgg ctgcctccgt
      481 gggccaaggc tctggtctgg gagcaggcgc tctggcgctg gatcgtgttc ggatcggccc
      541 ttctgactgt cctcggagcg gtgaccatcg tgcgccgctg gatcaaccgg aggggagatg
      601 tccacccgat tcgccaagac gtgcgtcgtc tggtgctacc ggtgtcggtg agcctcgccg
      661 cctggttcct gggctttgtc agcgacgcca tcaatatcac ggggcaggcg cgcgatgtga
      721 ttgccacggg cctcggcgcc atactcttcg tggccttggc ctggtccgtc ctgatgattg
      781 gcgagctcat cgccgagggc atcgtagctt caccccggtt caggacccgg agtctggatg
      841 aggcgatcgt ccggctgacc tcgcggatcc tggccctgat ctcggcggtt ctggttaccg
      901 tgtacggggc ccagtcgctt ggcctgccgg tggttccgct gttggcgggg ctcggggtcg
      961 gcggtctagc gctggccttg gcggcccagc cgacgatcga gaacctcatc gcggcgttga
     1021 cgctccatgc cgatcggccc gtacgcgtcg gcgatttctg tcgcttcggc gatgcgctgg
     1081 gcatcgtgga agagatcggc atgcgatcca cgcgcatccg cacgctggac cacacggtgc
     1141 tgtctgtgtc gaacgccgat ttttcgaagg gcagagtgga gaactactcg gcgcggagga
     1201 agatgtggta tcacccgagg atcaggctgc gctatgagac gacaccggac caggtccgct
     1261 acatcctggt cgagatacgc aagcttctgt acgcgcaccc gcgagtcttg ccggacccgg
     1321 cccggatccg attcgtgggc ttcggcgaat attctctgga tctcgacgtg ttcgcgtatg
     1381 tcgataccaa ggactacggc gagtatctcg agatcgccga ggacctaaat ctaaggatca
     1441 tggacgtcgt caaggctgcg gggaccgagt tggcgattcc ggcgcgactc gagtatcaaa
     1501 tggacaaggc cccgctggac gagaagggga tccagcaggc cgaagcccac gtcgaggaat
     1561 ggcgaggcca gcaggcgatg taccttccga aattcccacc cgagacgatc gccaacctgc
     1621 gcggatcgct cgactaccct ccggccggat cgcccgggat gaccggggag cgccaggagc
     1681 gtgatccttc ctcggacacc ccaaggccag cctcgggccg cagatcgtag agccgcgggg
     1741 gacggtcagc gctcggcgac gtgctcgcgc aggatgcgcc ggacctcgag gatcgtctcg
     1801 ggattggcct gggtggagtg gcccgaccgc acgaccttct cggaggccac cccgtcgaga
     1861 tgggcgctct cgtaggcgac gacgccgtcg gtcttgccgg tgaccgggcc gctgccgacc
     1921 acggcgatga tcgagtgagt cctcacgccg ggcgcgacgg ggcgctccgt gagggcccgg
     1981 atgaagcggt ggccggggcg catgttgtcg acggcggtcg ggacgtcgtc ggcctcgatc
     2041 gacagcgcat ccgggttgga ccggatgacg tcattgacgt cctggacgac cgtgac
//
LOCUS       DATITW010000080         1816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_4037, whole genome shotgun sequence.
ACCESSION   DATITW010000080 DATITW010000000
VERSION     DATITW010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4037"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VLS86_01115"
     CDS             complement(<1..474)
                     /locus_tag="VLS86_01115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSO49119.1"
                     /translation="MWADRDVTRVVAARFISRVGGEAAFFVGIWGKAAFELEATAAGL
                     AAVMAALGLAALIGSAIAGVLVDRFDPRRVLLGGELLFIPVALGLVFADTIPAMVVAT
                     FLLGLVGTPVYTAIASFGPFLTDDPERLAKVNGLIEGESWAAFVVGPAIGSIITIT"
     gene            complement(527..>1816)
                     /locus_tag="VLS86_01120"
     CDS             complement(527..>1816)
                     /locus_tag="VLS86_01120"
                     /inference="COORDINATES: protein motif:HMM:NF019626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HSO49120.1"
                     /translation="EGLLNEVDSYTQAIKELSDRIVEAQRPIRVLSAINWDDRIKAEF
                     FASGFKEQPKVDGDFYQAKELKLDPEETRAAFRSIEGDILGRLGPVAPAGVMMRFMCE
                     QYRLTVDLLEARGTNHFTQISGLLSGTPFDVFHVGGPTISDLAKQMRHILEGLAGETM
                     EMVDDRSIDGRTAVGILQSQLDDSMGAGVIDVRADDGIAADAAAGADYIKLNEDRFFS
                     QRDLDLLEAHEGWVHVGTTLNGLAQPVCTFLGKAAPRSTVTQEGLAVLTEMINMRSHP
                     RRLARLMHRIEGIERAAEGATFLDVFESFRTDGLDEEESWSAAHRIFRGSLPDAGPFT
                     KDLGYGKGLVLTLLYVRLAIQSGRAHRIPLLFCGKVDLLDMGVLHQLEEEGLVQQPSF
                     VPPPFNDLPALAVALSLGGFTAKLDWTRLESDYSGLI"
BASE COUNT          350 a          625 c          551 g          290 t
ORIGIN      
        1 cgtgatcgtg atgatcgacc cgatcgcggg gccgaccacg aatgcggccc aagactcacc
       61 ctcgatcaaa ccgttgacct tggccagccg ctccgggtcg tcggtcagga acggcccaaa
      121 cgacgcaatg gcggtgtaga caggcgtgcc gacgagcccg agcaggaagg tggcgaccac
      181 catcgcgggg atggtgtcgg cgaagaccag tcccagggcc accgggatga aaagcaattc
      241 gcccccgagc aacacccggc gcgggtcgaa ccggtcgacg agcaccccgg caatggctga
      301 gccgatcaac gccgccaggc cgagggcggc catcacagcg gccagaccgg ccgccgtggc
      361 ctccaactcg aaggcagcct tgccccagat gccgacgaag aaggccgctt cgccaccgac
      421 tctggagatg aaacgggcgg caaccaccct cgtgacatct cggtcggccc acatggcggc
      481 cacggtaccg ccccctcccc cggccctgcc gcgggataag aaggtgctag atcagccccg
      541 agtagtcact ctccaaccgg gtccaatcca gctttgccgt gaacccgccg agactgagcg
      601 ccactgccag cgcggggagg tcgttgaacg gcggaggaac gaacgacggt tgttggacca
      661 gaccctcttc ctccaattga tgaagcaccc ccatgtccaa cagatccacc ttcccgcaga
      721 acagcaacgg gatgcgatgc gccctaccgc tctgaatcgc cagccggacg tagagaagcg
      781 tcaggaccag tcccttgcca tacccgaggt ccttggtgaa cggccccgcg tccggaaggc
      841 tcccccggaa gatccgatgc gccgcggacc acgactcctc ctcgtcgagg ccatccgtgc
      901 gaaacgactc gaagacatcg agaaaggtgg ctccttcggc ggcgcgctcg atcccctcga
      961 tccgatgcat caacctcgcc agcctcctcg ggtggctgcg catgttgatc atctcggtga
     1021 ggaccgccag tccttcctgg gtcacggtcg accggggagc cgccttgccg aggaaagtgc
     1081 acaccggctg agccaagccg ttgagggtgg tcccgacatg gacccacccc tcgtgagcct
     1141 ccaacaggtc gaggtcgcgc tgtgaaaaga accggtcctc gttgagcttg atgtagtcgg
     1201 ctcccgctgc agcatcggcc gcgatcccgt cgtcggcccg gacatcgatc accccggcgc
     1261 ccatcgagtc gtcgagctgg ctctgcagga ttcccaccgc ggttcgcccg tcgatcgagc
     1321 ggtcgtcgac catctccatg gtctcaccgg cgaggccctc gaggatgtga cgcatctgct
     1381 tggcgaggtc ggagatggtt ggccctccga catggaacac gtcgaacggc gtgccggaga
     1441 gcagcccgct gatctgggtg aagtggttcg tgccccgagc ctcgagcaga tcgacggtca
     1501 atcgatactg ctcacacatg aatctcatca tcaccccagc cggggcgact gggccgagac
     1561 gcccgagaat gtcgccctcg atggagcgga aggcggcgcg ggtctcctcc ggatcgagtt
     1621 tgagctcctt cgcctggtag aagtcgccat cgaccttcgg ctgctccttg aagccgctgg
     1681 caaagaactc ggccttgatc cgatcatccc agttgatcgc gctcaacacc cggatcggtc
     1741 gctgcgcctc gacgattcga tccgagagct ccttgatggc ctgggtgtaa gaatcgacct
     1801 cgttcaacaa cccctc
//
LOCUS       DATITW010000081         1517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_604910, whole genome shotgun sequence.
ACCESSION   DATITW010000081 DATITW010000000
VERSION     DATITW010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604910"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VLS86_01125"
     CDS             <1..672
                     /locus_tag="VLS86_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HSO49121.1"
                     /translation="RLLPFRSPAALEVFRYAEDVALERRQKPEDDIITVLLTAEPDGQ
                     RLTDLEFKNFFTLMMVAGNETTRHTISHGLTYLIDHPEQMEQLRDNPAGLSATATEEI
                     LRASAVTMHFRRTATEDVEMRGASIKAGDRVVMWYPSANYDEEVFEDPFRFDIRRDPN
                     DHLTFGTGRHVCLGASLARLEVRVVFEELLKRVASIELTAEPDRLRSNFINGIKHLPV
                     RVSQV"
     gene            complement(669..1376)
                     /locus_tag="VLS86_01130"
     CDS             complement(669..1376)
                     /locus_tag="VLS86_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49122.1"
                     /translation="MSDGRPAFYARPGSSWDDLVSLLHVPYTLWHLSYVVIGAALAPT
                     VDWLILSGTLAAFAVGLGIGAHALDEVKSRPLSTGLTDRVLWLIGIGAMVVATGIAVV
                     GSFQVSPWVMAWGVVGVFLAVGYALEWPVIHTDLGFGLAWGAFPVVVGYWAQTQAISV
                     SVVVAAAAATLLSLAQRALSTPARFVRRRTRESVVEFDGEVRWDRAELLQTWERPLRL
                     LAWAMVALALGLISTHL"
BASE COUNT          280 a          529 c          487 g          221 t
ORIGIN      
        1 cgcctgttgc cgttccgctc tccggccgca ctcgaggttt tccgctacgc cgaggacgtg
       61 gctctggagc gacgccagaa gcctgaggac gacatcatca ccgtgctcct gactgccgag
      121 cccgacggcc agcggctcac cgacctcgag ttcaagaact tcttcaccct gatgatggtg
      181 gccggaaacg agacaacgag gcacaccatc agccacgggc tcacctacct catcgatcat
      241 ccggagcaga tggagcagct gcgcgacaac ccggcagggc tgtcggcgac agcaaccgag
      301 gagatcctcc gtgcgtcggc ggtcaccatg cattttcggc gaacggccac cgaggatgtg
      361 gagatgcgcg gcgcctcgat caaggcgggg gaccgcgtgg tcatgtggta cccgtccgcc
      421 aactacgacg aggaggtgtt cgaggacccg ttccggttcg acattcgacg tgatccgaac
      481 gaccacctca cctttggcac cggccgccat gtctgtctcg gggcttccct tgcccgtttg
      541 gaggtgaggg tggtcttcga ggagctgctg aagcgcgtcg cctcgatcga gctgaccgcg
      601 gagccggatc gtcttcgctc caacttcatc aacgggatca agcatctccc ggtgcgggta
      661 tcgcaggtct agaggtgggt cgagatcagg ccgagggcaa gggccaccat cgcccaggcc
      721 agcaggcgaa gcggccgctc ccacgtctgc agcaactcgg cccggtccca tcgcacctcg
      781 ccgtcgaatt ccacgaccga ttcccgggtg cggcgtctga cgaaccgggc cggtgtgctc
      841 aacgccctct gcgccaggct gagcagggtg gcggccgccg cggccaccac gaccgacacc
      901 gatattgcct gggtttgggc ccaatacccg acgacgaccg ggaacgctcc ccaggccagc
      961 ccgaacccga gatcggtgtg gatcacgggc cactccaggg cgtagcccac ggcgaggaag
     1021 acaccgacca ctccccaagc catgacccat ggtgacacct ggaaagaccc gaccactgcg
     1081 atccccgtcg ccaccaccat cgcgccgatc ccgatcagcc acagcacccg gtcggtgagg
     1141 cccgtgctca agggccggct cttgacctcg tccagggcgt gggccccgat cccgagcccg
     1201 accgcgaacg cggccagggt tccggagagg atcagccagt cgacggtcgg ggccagggcg
     1261 gcgccgatca ccacatagga cagatgccac aaggtatagg ggacgtggag cagagagacg
     1321 agatcgtccc agctcgagcc gggtcgggcg tagaaagccg ggcgcccgtc gctcaccgct
     1381 tgcgcccggt catcaccagc ccaccgccca aggagagccg ggtcacctcg acttcggcca
     1441 gccccgccga ctgccagatc tcgctcaggc gcgaggcggg gtagcgcgtg gcgaagtcgt
     1501 cgatgctggg cccgaga
//
LOCUS       DATITW010000082         2945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_604927, whole genome shotgun sequence.
ACCESSION   DATITW010000082 DATITW010000000
VERSION     DATITW010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604927"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /gene="coaBC"
                     /locus_tag="VLS86_01135"
     CDS             complement(<1..1169)
                     /gene="coaBC"
                     /locus_tag="VLS86_01135"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HSO49123.1"
                     /translation="MLQGRRVLLCVTGGVAAYKSAYLARRLIEAGAVVRVEMTDSALE
                     FVGPQTFAAITGEHPFTSLFAADLVSPHTELARWADIVIVAPATAATIGRVANGISSD
                     LVSSTLLATTAPVLLAPAMHTEMWEHPATRRNLELVTGFGYHLVGPNPGELAGRDTGI
                     GRVAEPEEIVEAAGDILSGDGAGRRLLVTAGGTREPLDPVRFLGNRSSGKMGHSIADA
                     AIRRGYEVTLVTTADLPVHPAVKVVRVETADEMLAAVSGTQPEIAVMAAAVADFKPAV
                     SADEKIPRADGLDSVPLVPTPDILASLVGREPRPFVVGFAAETGGVERAIEKAKRKEV
                     DLMVYNDVTEPGSGFGTDTNRVVLIGSEGDTEELPLLPKQEVAEKLLDRIGSAMG"
     gene            complement(1169..1390)
                     /gene="rpoZ"
                     /locus_tag="VLS86_01140"
     CDS             complement(1169..1390)
                     /gene="rpoZ"
                     /locus_tag="VLS86_01140"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HSO49124.1"
                     /translation="MKIEDVIDQIGNRFATVVVAARRARQINGYFHQLGVGFGEFVPP
                     QVHSLSRKPLTIAMEEIAEGKVSVDTVEA"
     gene            complement(1430..1981)
                     /gene="gmk"
                     /locus_tag="VLS86_01145"
     CDS             complement(1430..1981)
                     /gene="gmk"
                     /locus_tag="VLS86_01145"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HSO49125.1"
                     /translation="MAGRLLVVSGPSGSGKSSIVRELLDRLDVEFSVSVTTRLPRPGE
                     RHGDHYNFVSRRDFEAMIENGELLEWAIYNNRFYGTPAAPIEQAVAEGRDILLEIEIQ
                     GARQVREQRPDAVMFFVAPPSMDELERRLRRRGDTSEEDIEDRLEIAEQEMQEAPILF
                     DHVIVNDDLDRAIEDIVGLITAD"
     gene            complement(1983..2297)
                     /gene="mihF"
                     /locus_tag="VLS86_01150"
     CDS             complement(1983..2297)
                     /gene="mihF"
                     /locus_tag="VLS86_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HSO49126.1"
                     /translation="MAQPPQLTDEQRAAALVKAAEARRVRAEVKELLKTGSLRLSELI
                     DKAGSDDILAGLKVERVIASMPGTGKIKAKRMMESLGIAENRRIRGLGEKQKEALLAE
                     FS"
     gene            complement(2345..>2945)
                     /gene="pyrF"
                     /locus_tag="VLS86_01155"
     CDS             complement(2345..>2945)
                     /gene="pyrF"
                     /locus_tag="VLS86_01155"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HSO49127.1"
                     /translation="LLMGEGSAIVSRVADLGLPVFVDAKLHDIPNTVGRAAERLGSLG
                     ARWVTVHASGGREMVRVAAEALNESSGGTAGVLAVTILTSLGEYELAEIGIERPLDEL
                     VSSLARSAAAGGAEGVVCAVTEARLVQALGLGLIVVTPGIRPADVGASDQKRVASPAT
                     AIRAGSDLLVVGRPSTSAPDVVAAAIAICEEVEAAVSTP"
BASE COUNT          488 a         1039 c          924 g          494 t
ORIGIN      
        1 tcccccattg ccgagccgat gcggtccaac agcttctccg ccacttcttg cttgggcagc
       61 aagggcagct cctcggtgtc accttcgctg ccgatgagga ccacccgatt ggtgtcggtg
      121 ccgaacccgg agcccggctc ggtcacgtcg ttgtacacca tcaggtcgac ctccttccgc
      181 ttcgccttct cgatggcccg ttccacccct ccggtttccg cggcgaagcc gaccacgaac
      241 gggcggggct cacgacccac cagcgaggcg agaatgtcgg gggtcggcac cagtggcacg
      301 gagtcgagac cgtcggcgcg cgggatcttc tcatcggcgc tgacagcagg cttgaaatcg
      361 gcgaccgcag cggccatgac ggcgatctcc ggttgcgtcc ccgacacggc cgccaacatc
      421 tcgtccgccg tctccacccg caccaccttc accgccgggt gcaccgggag atcggcggtc
      481 gtgaccaggg tgacttcgta tccacggcga atcgcggcgt cggcgatgga gtgacccatc
      541 ttccccgaag accggttgcc cagaaaccgc accggatcca gcggctcgcg ggtcccaccc
      601 gcggtgacca gcagcctcct ccccgcacca tcaccgctca ggatgtcgcc ggccgcctcc
      661 acgatctcct cgggctccgc cactctcccg atcccggtgt ctcgccctgc gagctccccc
      721 gggttggggc ccacgaggtg ataaccgaac cccgtcacca gctcgagatt gcgacgggtc
      781 gccgggtgct cccacatctc ggtgtgcatg gcgggagcca gcaacaccgg ggcggtcgtg
      841 gcgagcaacg tcgagctgac caggtcagag gagatgccgt tggccaccct gccaatcgtg
      901 gccgcggtcg ccggggccac gatcacgatg tcggcccacc tggccagctc ggtgtgtggg
      961 gacacgaggt cggctgcgaa cagcgaagtg aacgggtgct caccggtgat ggcggcaaag
     1021 gtctgcggcc cgacgaactc gagagcggag tcggtcatct ccacccggac cactgccccg
     1081 gcttcgatca ggcgccgggc caggtatgcg gacttgtagg cggcaacgcc acccgtgacg
     1141 cagaggagga cgcgtcgccc ctggagcatc aggcctcgac ggtgtcgact gagaccttgc
     1201 cctcggcaat ctcttccatg gcgatggtca gcggcttgcg cgacagcgag tgaacctgtg
     1261 gcgggacgaa ctcgccgaaa cccacaccta gctggtggaa gtacccgttt atctgccggg
     1321 cgcggcgggc ggcgacaacg accgtcgcga agcggttgcc gatctggtcg atgacgtctt
     1381 cgatcttcat gggggtcctc agtggggaga caagggacag agtacccgat cagtcggcgg
     1441 taatcagccc cacgatgtct tcgatcgccc gatccaggtc gtcgttgacg atcacatggt
     1501 cgaacaagat cggtgcctcc tgcatttcct gctctgcgat ctcgagccga tcctcgatgt
     1561 cctcttccga tgtgtcacca cgccggcgga gacgccgctc caactcgtcc atcgatggcg
     1621 gcgcgacgaa gaacatcacc gcgtccggtc tttgctccct gacctggcgg gcgccctgga
     1681 tctcgatctc gagcaagatg tcgcgccctt cggccacggc ttgctcgatg ggtgcggccg
     1741 gcgtcccgta gaaccggttg ttgtagatcg cccactcgag cagctcgccg ttctcgatca
     1801 tcgcctcgaa atcgcgtcgt gagacgaagt tgtaatgatc gccgtgtcgc tcacctggcc
     1861 tggggagacg ggtggtgacg gagacggaga actccacgtc gaggcggtcc agcaactccc
     1921 tcacgatgct cgactttccc gagcccgagg gcccggacac gacgaggaga cgaccagcca
     1981 cgtcaggaga actcggctag gagagcctct ttctgctttt ctcccagacc cctgatccga
     2041 cgattctcgg cgattccgag tgactccatc atcctcttgg ccttgatctt cccggtgccg
     2101 ggcatcgagg cgatcacacg ctcgaccttg agaccggcga ggatgtcgtc gctcccggcc
     2161 ttgtcgatca gctcggagag acgaagcgac ccggtcttca gcaactcctt gacctcggcc
     2221 ctcacccggc gggcctcggc ggccttgacc aaggcggcag ctcgttgttc gtctgtcagc
     2281 tggggtggtt gggccatgtc ctcatctcgc gtcgaaagcg cgaccatagc caccacggtc
     2341 ggggctacgg ggtcgaaacc gccgcctcga cctcttcgca gatcgcgatc gcggccgcga
     2401 cgacgtccgg tgccgatgtg ctcgggcgcc caaccaccag caggtccgag ccggcgcgga
     2461 tcgcggtcgc cggagacgcc acacgcttct ggtcggaggc tccgacatca gcgggcctga
     2521 tcccgggagt gaccactatc aggccgagac ccagggcctg gaccaatctg gcctccgtga
     2581 ccgcacagac cacaccctcg gcgccaccgg ccgccgcgga tctggcgaga gacgagacca
     2641 gctcgtcgag tggccgctcg atcccgatct cggccagctc gtactcgccg agcgaggtga
     2701 ggatggtgac ggcgaggact ccggccgtgc cccccgagct ctcgttcagc gcctcggcgg
     2761 ccactctgac catctcccga ccccctgagg cgtgcacggt gacccacctc gcccccaggg
     2821 agcccagacg ctccgcggcc cggcccacgg tgttgggaat gtcgtgaagc ttggcgtcga
     2881 cgaacaccgg caggccgaga tcggccaccc gtgacacgat cgcggacccc tcccccatca
     2941 agagc
//
LOCUS       DATITW010000083         1730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_765379, whole genome shotgun sequence.
ACCESSION   DATITW010000083 DATITW010000000
VERSION     DATITW010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765379"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..350
                     /locus_tag="VLS86_01160"
     CDS             78..350
                     /locus_tag="VLS86_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HSO49128.1"
                     /translation="MAWTRELPPCEHLGSIHEVEPVSTGCAECLAVGDTWVHLRLCLS
                     CGNVGCCDQSKNTHARKHAGTSEHPIVRSFEVGETWRWCYPDNRFV"
     gene            402..>1730
                     /locus_tag="VLS86_01165"
     CDS             402..>1730
                     /locus_tag="VLS86_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017300127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSO49129.1"
                     /translation="MPKPVILTVDDDPEVLRAVQRDLRRHYSEEFRVVAASSGTEALE
                     ALRDLNLRNEPVALLLVDQRMPEMSGVQFLAQTLERLPDAKRALLTAYADTEAAISAI
                     NEVSLDHYIMKPWDPPEERLYPVLDDLLEDWWDHFTPRFEGISVIGHRWGRATHEIRA
                     FLARNQVPYRSLDLGTDEEAETLLTDVGGDPTLLPVVVMPDGVVLVQPTSADVAGRIG
                     LKTQPTERSYDLVIVGAGPSGLAAAVYGASEGLATLLVESEAPGGQAGQSARIENYRG
                     FPKGLSGSDLTRRAVTQATRFGAEILTPAEVVSVTRNDPFRTVGLNDGTEVSTKALLI
                     TTGVSYTKLDAPGIEDLTGAGVYYGTSRIEGAAHRDEPMFIIGAGNSAGQAAMFLSRF
                     GSSVTLLVRGSSLQEFMSQYLIDAIAAEDNIFVRYSTQLLEARGDSHLEQL"
BASE COUNT          315 a          600 c          525 g          290 t
ORIGIN      
        1 ggttagggcg gtgtcgcact gcaatcgcca aacgcctaga ttccggcacg aggtttcgga
       61 gaggcaggga aatcaccatg gcatggaccc gcgagttacc accgtgcgag catctcggct
      121 cgattcatga ggtggaacca gtcagcacgg gctgcgccga gtgcctggct gttggagata
      181 cctgggttca tctccgtctc tgtctcagct gtggcaatgt cgggtgttgc gaccaatcga
      241 agaacacaca cgcccgcaag cacgccggaa ccagcgagca cccgatcgtg cgctcgttcg
      301 aggtcggcga gacctggcgc tggtgttacc ccgacaaccg cttcgtctga gatgcctatg
      361 tcggctcccc tgacccgacc cctcggcctc ggttcaccct gatgcccaag ccagtcatcc
      421 tcaccgtcga cgacgacccc gaagtcctca gagccgtcca gcgcgacctg cgacgccact
      481 acagcgagga gttcagagtc gttgccgcca gttccggcac cgaggccctc gaggccctga
      541 gagacctcaa cctccggaat gagccggtcg ccctgctctt ggtggatcag cgcatgcccg
      601 agatgtcggg ggtccagttc ctggcccaaa ccctggagcg cctgccggat gccaagcggg
      661 cgctgctgac ggcatatgcc gacaccgagg cggcgatctc cgcgatcaac gaggtttcac
      721 tggatcacta catcatgaag ccctgggatc cccccgagga gcgcctctat ccggtgctcg
      781 acgacctcct cgaggactgg tgggaccact tcaccccgcg tttcgaggga atcagcgtga
      841 tcggtcatag atggggccgc gccacccacg agatccgggc cttcctcgcc cgcaaccagg
      901 tcccctaccg atccctcgac ctcggtaccg acgaggaggc ggaaaccctg ctcaccgacg
      961 tcgggggcga ccccaccctg ctcccggtgg tggtcatgcc ggatggggta gttctcgtcc
     1021 agccgacatc tgcggacgtc gctggtcgca tcggcctgaa aacccagcca accgagcgct
     1081 cctacgacct cgtgatcgtc ggcgccggcc cctctggtct cgccgccgcg gtctacggcg
     1141 cctcggaggg gctggccact ctcctggtgg agagcgaggc acccggaggg caggccggtc
     1201 agagtgcccg aatcgagaac taccgtggat tcccaaaagg cctcagcgga agcgacctga
     1261 cgagacgagc ggtcacccaa gccacccgct tcggagcaga gatcctcacc ccggccgagg
     1321 tggtgtcggt cacccggaac gacccgttcc gaacggtggg actcaacgac ggcaccgagg
     1381 tgagcaccaa ggccctcctc atcaccaccg gagtctccta caccaagctc gacgcgcccg
     1441 gaatcgaaga cctgaccggg gcgggggtct actacgggac ctcccggatc gagggcgccg
     1501 cccatcgtga cgagccgatg ttcatcatcg gagccggcaa ctccgccggc caggccgcta
     1561 tgttcctctc ccggttcggc tccagcgtga ctctgctggt gcgtggatcc tcactccagg
     1621 agttcatgtc gcagtacctg atcgatgcaa tcgcggccga ggacaacatc ttcgtccgct
     1681 acagcaccca gctgctcgag gctcgtggtg acagtcatct cgagcagttg
//
LOCUS       DATITW010000084         7876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_44408, whole genome shotgun sequence.
ACCESSION   DATITW010000084 DATITW010000000
VERSION     DATITW010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7876
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44408"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VLS86_01170"
     CDS             <1..344
                     /locus_tag="VLS86_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSO49130.1"
                     /translation="DAVAAAGGALEGAVLESLNLASPVLDGDHIQIPGPGDVIAATGG
                     GEEGDGRIALNRADAAALEQLPGVGPVLAERIVAHREANGPYQEVEDLLEVSGIGEAK
                     LASIRDLIVVP"
     gene            341..2416
                     /locus_tag="VLS86_01175"
     CDS             341..2416
                     /locus_tag="VLS86_01175"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HSO49131.1"
                     /translation="MSIAHPALPPPVSRPWVAGAVLTFTVAVTVSTSARALPLISLPV
                     LWLVLKGRVVLAVLATLAAVTSLVASRPLEPLAPGPISGEAVLATDVTQGRFGPYALV
                     EMGDGLILANLPMGAEGALGDLVWLQGSVVGDPGAIKGRRYRGAVGVSEFEVLSGARS
                     PVMAMGNAIRDRVTTRLAPLEEGRGLLAGFLVGDTSGVDEIDQAAMRRAGLSHFTAVS
                     GSNVALFLGLVFVALGPIGVGPRRRAIVGLLALPVFAAATRFEPSVLRASAMAALALG
                     GRLVGLAMEAWQILSAAVVGLVLLEPMLVKSIGFQLSVAATAGVMVGARWPVPPTRSA
                     RALAVAFGAQTAVAPLLALHFGVVPLLSPVVNLVAAPLVGVSTILGAVGMVGPVQLTS
                     LGAWLAGGVLWLARVAAGWPQIGAVGVLVSLMWGLLYLAMPRLRGALALTAGLLVASL
                     VVGGTDPVPTPGVVVLDVGQGDSILVSGGAGRYALVDGGPDPVRLLESLRRYGVTQLE
                     LVVLTHVHADHATGLSGLVGRIPIGRVWASVEPHRSPASTELFRLLEAAEVSIDQPQP
                     GTRWELADLTLRVEGPLRRYASPNDQSVVLLVEGPSQSLLLSGDIETVAQAELGHLRA
                     DVLKVPHQGAATSDPDWLREVGSELAVISVGPNDFGHPAAWVVELLESSGADVVRTDE
                     QGDVPVPLS"
     gene            2445..3269
                     /locus_tag="VLS86_01180"
     CDS             2445..3269
                     /locus_tag="VLS86_01180"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HSO49132.1"
                     /translation="MAGVLDLGEARLPLGESTYVMGVINVSPDSKNVHTVARSVDAAL
                     LMADRYRAWGATLIDLGGQSSHFDNPTLEVADEVSLVVPVIEALSAEGHLVAIDTWKP
                     EVADAAVDAGAVIVNDTGGLADPEMREVVAATGVAAIAVYVEGENPHDVGEIEMSDDK
                     AEQTAAGFRALLADLAESGIHEVIFDPGIALNYRGDYEAYTRMQLQVIAGSEMIRALG
                     RPLLVPIPRKRDFHRVVAYMALALEHRADIIRVHDVAVACDLADLFGRKAPALGLG"
     gene            3300..4283
                     /locus_tag="VLS86_01185"
     CDS             3300..4283
                     /locus_tag="VLS86_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49133.1"
                     /translation="MGLLLFSAPANAGPGERQQMLDAAAAHFTRCGLERSDLLRIDVP
                     GRGAGEEGDGALRAELEPVIPLLQSGSLFGVAQGLLLVDAQNLQASEGEVLDTLIRAA
                     DPTSVEVVLLATGAVPKTLAALVKQIGSSQSVTKMWERQARQWLDAEIRDRGLVFEKG
                     AEKALLQKFGSDTASMGQALDQLEQTTGKITKDLILDRFKNRPDEPTFLITDAISKGE
                     VGEALRRLSDFLVHGHPLVFLAAVESDLKKRALAAAAPDETTLREWLGARPSDRGPTR
                     VWSQRGRVRESSLRRAQEALVRADRVIKSQPEDVHLVTLERLTVALCRWYG"
     gene            complement(4319..4570)
                     /gene="rpsT"
                     /locus_tag="VLS86_01190"
     CDS             complement(4319..4570)
                     /gene="rpsT"
                     /locus_tag="VLS86_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HSO49134.1"
                     /translation="MANIKSQIKRNRQNEHRRQRNKSVNSSLKTSVKKVEAAALAGEP
                     TEDLLKAAQSKIDNAVSRGVLHKKTAARKKSRLAKRVSA"
     gene            4681..6489
                     /gene="lepA"
                     /locus_tag="VLS86_01195"
     CDS             4681..6489
                     /gene="lepA"
                     /locus_tag="VLS86_01195"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HSO49135.1"
                     /translation="MTNQTRIRNFSIIAHIDHGKSTLADRLLERTGAVTDRDMREQVL
                     DTMDLERERGITIKAQTVRLNHVAADGEEYVLNLIDTPGHVDFSYEVSRSLAACEGAL
                     LLVDAAQGVQAQTLANAYLAINAGLEVIPAINNIDLVAADPDRVAGEITNVIGGTAED
                     IVRLSAKTGDGVDELLQRLVDQVPAPRGEPDAPLRALVFDSYYDAYRGVVCYVRVVDG
                     RLRSGARLKFIATGESHDAAEIGVLTPSAKPVNLLEAGEVGYLITGVKEIDRIRVGDT
                     LTDAASPARDPLPGYAEPRSMVFSGLFPTDGDDFERLREALEKLRLNDGSLMWEPETS
                     RALGFGFRVGFLGLLHMEIVRERLEREYDLDLVATAPSVAYQVTLTDGTVREIKSPQD
                     LPDPSGRRGVSEPYVRAMILTPTEFVGPIMELVTTKRGDLANMSYLSPERVELDYKIP
                     LAEIVFDFFDLLKSRTKGYASFDYEPIEYREADLVKVDILLNGQPVDAFSAVVHRDKA
                     ADYGKTMTTRLRQLIPRQMFDVPIQAAIGGKIISRETVRAKRKDVTAKCYGGDVSRKK
                     KLLQKQKEGKKRMKMVGSVEVPQEAFVAALRVDDEL"
     gene            6501..7694
                     /gene="hemW"
                     /locus_tag="VLS86_01200"
     CDS             6501..7694
                     /gene="hemW"
                     /locus_tag="VLS86_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HSO49136.1"
                     /translation="MDHHVNSTAAYVHIPFCSAVCPYCDFAVVAGADHLAERYVEALV
                     AEIERGEVWLPLDSVYVGGGTPSHIDPRLLGRILETLSRHHGLAGDAEVSLEANPEDF
                     SLDHARRLASHGFNRVSFGAQSFDDAVLARLGRRHRGPDIVRSVENARTAGIRSVSLD
                     LIFGTPVESDESWERSLRSAIALGPDHVSCYALTVEPGTPLNREVLSGAAAPDPDVQA
                     DRYELADEMLGSSGLQRYEVSNWSRPGHRCRYNLTVWAQGEYEAYGHGAHRFRRGIRS
                     HNIRRLEAYLDRVEQGESPIAGGEPIHGWDRELDRLFVGLRRSDGVASGPGVAALLET
                     PGGEILVRERVIELRAGRLVVSRPLLTDEVHRQVLSLDPPIGWVEAPNPDNLLADRDA
                     GTTSL"
     gene            7672..>7876
                     /locus_tag="VLS86_01205"
     CDS             7672..>7876
                     /locus_tag="VLS86_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HSO49137.1"
                     /translation="MLEQRRSEILRALVEEHIRSGEPVSSRAIVERAHLSVSTATVRH
                     DLTSLEAEGFVIQPHTSAGRIPTA"
BASE COUNT         1234 a         2489 c         2735 g         1418 t
ORIGIN      
        1 ccgatgcggt ggccgcggcc ggtggggccc tcgagggagc cgtgctcgag tctctcaacc
       61 tggcctctcc agtcctcgac ggggaccaca tccagatccc gggcccaggc gacgtgattg
      121 ccgccacggg tggtggggaa gagggcgacg ggcggatcgc gctgaatcga gccgatgctg
      181 ccgctctcga gcaacttccc ggggtggggc cggtgctcgc tgaacgcatc gtcgcccacc
      241 gggaggcgaa cgggccgtat caagaggtgg aggacctgct cgaggtgtcc ggcatcggtg
      301 aggccaagct ggcctcgatc cgggacctga tcgtcgtccc ttgagtatcg cccatcccgc
      361 attgccgccc ccggtgtcgc gtccttgggt cgccggggcg gtgctgacct tcaccgtcgc
      421 cgtgacggtc tcgacctcgg cccgggcgtt gcccttgatc tcactccccg tgctctggct
      481 ggtgctgaag ggccgggtcg tgctggccgt cctggcgaca cttgccgccg tcacgtcgct
      541 tgtggcgtcg aggccactgg aaccactcgc ccccggcccg atcagtggag aggcggtcct
      601 cgccacggat gtcacccagg ggaggttcgg gccctacgcc ctcgtcgaaa tgggagacgg
      661 cctgatcctg gccaatctcc cgatgggcgc cgaaggggcc cttggggatt tggtgtggct
      721 gcagggatcg gtggtgggag atccgggcgc gatcaagggt cgccgctatc gcggcgcggt
      781 tggggtgagc gagttcgagg tcttgtccgg ggcgagatcg ccggtgatgg ccatgggcaa
      841 cgcgatacgg gaccgggtca ccacccggct cgccccgctg gaagagggac ggggtctgct
      901 ggcaggcttt ctcgtgggtg acacatctgg agtggacgag atcgaccagg cggcgatgcg
      961 tcgggcgggt ctgtcccact tcacggctgt cagcggctcg aacgtggcgc tgtttctcgg
     1021 gctcgtcttc gtggccctgg ggccgatcgg cgtcggtccg cgacgtcgtg cgattgtggg
     1081 tcttctcgct cttcccgtgt tcgccgccgc tacccggttc gagccgtcgg tgctcagggc
     1141 gtcggcgatg gccgccctgg ctctcggtgg gcgtctggtc gggttggcga tggaggcgtg
     1201 gcagatcctc tcggccgccg tggtcgggtt ggtgctcctc gagccgatgt tggtgaagag
     1261 cattgggttt cagctctcgg tcgccgccac cgcgggggtc atggtcgggg cacgatggcc
     1321 ggtgccgccg accaggtcgg ctcgggcgtt ggccgtggcc ttcggagccc agacggcagt
     1381 ggcgcccttg ttggccctcc attttggggt ggtgcccctt ctctccccgg tggtcaacct
     1441 ggtggctgcg ccacttgtgg gcgtctccac gattctcggg gccgtcggca tggtcgggcc
     1501 ggtgcagctg acgagcctcg gggcctggct ggccggcggc gtgctgtggc tggctcgtgt
     1561 cgctgccgga tggccacaga tcggggcggt cggtgtgctg gtgagcctga tgtgggggct
     1621 gctctatctg gcaatgccgc gactgagggg cgccctggcc ctgaccgcgg gtctcctcgt
     1681 tgcctcattg gtggtcggcg gcaccgaccc cgtgcccacc ccgggtgtcg tggtcctcga
     1741 cgtgggtcag ggcgactcga tcctcgtctc gggcggggcc ggtcgatacg cactcgtcga
     1801 tggcgggccc gacccggtgc gactcctcga gagtctccgc agatacggcg tgacccagct
     1861 cgagctcgtg gtgctgaccc acgtccatgc cgatcacgcc accgggctgt cgggtctggt
     1921 ggggcggatc ccgatcggac gggtctgggc cagcgtcgag cctcaccggt cgccggcctc
     1981 caccgagttg tttcgccttc tcgaagctgc cgaggtgtcg atcgaccaac cccagcccgg
     2041 gacgcgatgg gagctggccg atctgacctt gagggtcgaa ggcccgttgc gacgctacgc
     2101 ctccccgaac gatcagtcgg tcgtgctcct ggtggaaggc ccctcgcagt cgttgcttct
     2161 ctccggtgac atcgaaaccg tggcccaggc ggagctgggc catctgcggg cggatgtgct
     2221 caaggttccc catcaagggg cggctacctc ggatccggat tggctgcggg aggtcggctc
     2281 tgagctcgcg gtgatctcgg tcggtcccaa cgacttcggc catccggcgg cgtgggtcgt
     2341 ggagctgctg gagtcgagtg gcgccgacgt cgtccgaacc gacgagcagg gtgacgtccc
     2401 ggtccccctg tcctgagcgg ggaaacgatg ggagactgac agcaatggcc ggagtgctgg
     2461 atctcgggga ggcgaggctg cccctcgggg aatcaaccta tgtcatgggc gtgatcaacg
     2521 tctcacctga ttcgaagaac gtccacaccg tcgcccgttc ggtggacgcg gcgctgctca
     2581 tggcagatcg ttaccgggcc tggggcgcca cgctgatcga tctcggggga cagtcgtcgc
     2641 atttcgacaa tccgacgctc gaggttgccg atgaggtcag cctggtggtg cctgtgatcg
     2701 aagcgctgtc ggccgaaggt catctggttg cgatcgacac ctggaaaccg gaggttgccg
     2761 acgctgccgt ggatgcgggt gcggtgatcg tcaacgacac cggcgggctt gccgaccccg
     2821 agatgagaga ggtggtagcc gccaccgggg tggccgcgat cgcggtctac gtggagggcg
     2881 agaatccaca cgatgtgggg gagatagaga tgagtgacga caaggccgag cagaccgcgg
     2941 ccggctttcg agccctgctg gccgacctcg cagagagcgg gattcacgag gtcatcttcg
     3001 accccggcat tgccctcaac taccggggcg attacgaggc ctacaccagg atgcaactcc
     3061 aagtgatcgc aggctccgag atgatccgtg ccctcggtcg ccctctcctg gtcccgatcc
     3121 cgcgcaagag ggactttcat cgggtggtgg cctatatggc cctcgccctg gagcacaggg
     3181 cagacatcat tcgggtgcac gatgtggcgg ttgcctgcga cctggccgat ctgttcgggc
     3241 ggaaggctcc agccctcggg ctcgggtgac tcgtcaccct ggggggttat cgtgagcaga
     3301 tgggcctgct cctcttctcc gcacctgcca acgccgggcc cggggaacgc cagcagatgc
     3361 tcgatgcagc cgcagcgcat ttcacacgtt gtggtctgga acggtccgac ctgctgagga
     3421 tcgacgtccc gggtcgcggc gccggcgagg agggcgacgg agccctgcgc gccgagctcg
     3481 agcccgtgat ccccctcctc caatcgggct cattgttcgg cgtggcccag ggcttgctgc
     3541 tggtcgacgc ccagaatctc caagcaagcg agggcgaggt gctcgacacc ctgatccgcg
     3601 ccgcggaccc gacgtcggtc gaggtggtgc ttctggcgac cggcgccgtg ccgaagacgc
     3661 tggcggcgct ggtcaagcag atcgggagct cacagtccgt gaccaagatg tgggagcgtc
     3721 aggcccgtca gtggctcgac gccgagatca gagaccgggg actggttttc gagaagggcg
     3781 cagagaaggc gctgctgcag aagttcggct ccgacaccgc ttccatggga caggcgctcg
     3841 accaactgga gcagacgacc gggaagatca ccaaagacct gatcctggac cgcttcaaga
     3901 atcggcccga cgagccgacc tttctcatca ccgatgccat ctccaaggga gaggtgggag
     3961 aggccctgcg tcgtctgtcc gatttcttgg tccatggcca tcctctggtg ttcctggccg
     4021 cggtggaatc ggacctgaag aagcgggcgc tggccgcagc cgcgccggac gagacgacac
     4081 ttcgggagtg gctcggtgct cgacccagcg atcgcgggcc gactcgggtc tggagccagc
     4141 gggggcgggt cagagaatcg tcactgcgcc gagcacagga ggcattggtc cgcgccgatc
     4201 gggtgatcaa gtctcagccc gaggatgtgc atctggtgac tctggagagg ctgaccgtcg
     4261 ccctgtgccg gtggtacggc tgaatctgcg agacgagggg accggccgtg acggctggtt
     4321 aggcgctgac tcgcttggcg agtcgggact tcttgcgagc tgccgtcttc ttgtgaagca
     4381 cgccacgaga aactgcgttg tcgatcttcg actgggccgc cttgagcaga tcctccgtcg
     4441 gctcgccggc gagggcagcc gcttcgacct tcttcacgct ggtcttcagg ctgctgttga
     4501 ccgacttgtt ccgctggcgc cggtgctcgt tctggcgatt ccgcttgatc tgggacttga
     4561 tgttggccat gttgaaagac tctcatccga ctcggggatg gcgccaccag gcgggtgctc
     4621 gccagaaacc agagagtagc agggccccaa tcgaagttac aatgggtcca ccgcactcct
     4681 gtgaccaatc aaacgcgcat ccgcaacttc tcgatcatcg cccacatcga ccacggaaag
     4741 tcgactctgg ccgatcgtct cttggagcgc accggcgccg ttaccgaccg ggacatgcgg
     4801 gaacaggtcc tggacaccat ggatctggag cgggagcggg ggatcacgat caaggcgcaa
     4861 accgtgcgtt tgaaccacgt ggcggccgac ggcgaggagt acgtcctcaa cctgatcgac
     4921 actccgggcc acgtcgattt ctcatatgag gtctcccggt cactggctgc ctgcgagggc
     4981 gcgctgcttc tggtcgacgc cgcccagggg gtgcaggccc agaccctggc caatgcctat
     5041 ctcgcgatca acgccgggct cgaggtcata ccggccatca acaatatcga tttggtagcc
     5101 gccgacccgg atcgggttgc gggcgagatc accaatgtca tcggtggcac ggccgaagac
     5161 atcgtcaggc tctcggcgaa gaccggtgac ggagtggacg agctactgca gaggctggtg
     5221 gatcaggtcc cggcgccacg cggcgagccg gatgcgccgc tgcgtgctct cgtcttcgat
     5281 tcctactacg acgcctaccg cggggtggtt tgttatgtcc gcgttgtcga cggacgtctg
     5341 cgttccgggg cccgcctcaa gttcatcgcc accggggaga gccacgacgc ggccgagatc
     5401 ggtgtcctca ccccgtcggc gaagccggtc aatctcctcg aggccggtga ggtgggatac
     5461 ctgatcaccg gggtcaagga gatcgaccgg atcagggtgg gggacaccct caccgatgcc
     5521 gccagccccg cccgggaccc gcttcccggg tacgccgagc ccaggtcgat ggtgttctcc
     5581 ggtctcttcc ccaccgacgg ggacgacttc gagaggctgc gggaagccct ggagaagctg
     5641 cgactcaacg atgggtcgct gatgtgggag ccggagacca gccgggcgct gggattcggg
     5701 ttccgggtcg gcttcctcgg gctgctccac atggagatag tccgcgagcg actggagcgg
     5761 gaatacgacc tcgatctggt cgccactgcc ccctcggtgg catatcaggt gaccctcacc
     5821 gacggcaccg tgcgcgagat caaatcccct caggatcttc ccgacccctc gggacggagg
     5881 ggggtctccg agccctacgt gagggcgatg atcctgaccc cgaccgagtt tgtgggcccg
     5941 atcatggagc tggtgacgac caagcggggg gatctggcca acatgagcta cctgagcccc
     6001 gagcgtgtgg agctcgacta caaaatcccg ttggcggaga tcgtcttcga cttcttcgac
     6061 ctgctcaagt cgaggaccaa gggatatgcc tccttcgact acgagccaat cgaataccgg
     6121 gaagccgacc tggtcaaagt cgacattctg ctcaacggcc agccggtcga tgccttctcg
     6181 gccgtagtcc atcgggacaa ggctgccgac tacggcaaga cgatgaccac ccggcttcgc
     6241 cagctgatcc cgcgtcagat gttcgacgtg ccgatccagg ccgcgatcgg aggcaagatc
     6301 atcagccggg agacggtcag ggccaagcgc aaggacgtca cggccaagtg ctacggcgga
     6361 gacgtcagca ggaagaagaa gctgctccag aagcagaagg aaggcaagaa gcggatgaag
     6421 atggtgggct cggtcgaggt cccccaggag gcattcgtgg ccgcactgcg cgtcgacgac
     6481 gagctgtgag ccggcacgac gtggaccatc acgtcaacag cacggcggca tatgtccaca
     6541 tcccgttctg ctcggcggtc tgcccctact gcgactttgc cgtcgtcgcc ggcgccgacc
     6601 atctcgccga gcggtacgtg gaggcgctgg tcgccgagat cgagagggga gaggtgtggc
     6661 tgcctctcga ctcggtctat gtcggtggcg ggaccccgag tcacatcgat ccccggcttc
     6721 tgggacggat cctggaaacg ctgagccgtc accacggcct ggccggagac gccgaggtct
     6781 cactcgaggc caatccggag gacttcagcc tcgatcacgc ccgacgtctg gcgtcacatg
     6841 gcttcaaccg cgtgtccttc ggtgctcaga gcttcgacga cgcggtcctg gccagactgg
     6901 ggagacggca ccgcgggccg gacatagtcc gttccgtgga gaatgcccgg accgccggga
     6961 tccggagtgt gtcgctcgat ctcatcttcg ggacgcccgt ggagagcgac gaatcatggg
     7021 aacggtcttt gcgcagcgcc atcgccctgg gtcctgacca cgtctcctgc tatgccctca
     7081 ccgttgagcc cggcaccccg ctcaaccgtg aggtcttgtc cggggcagcc gcgcccgacc
     7141 ctgacgtgca agccgaccgt tacgagttgg ccgatgagat gctcgggagc tccggtctgc
     7201 agcgctacga ggtctccaac tggtcgagac ccggccaccg gtgccgctac aacctgaccg
     7261 tgtgggccca gggcgagtac gaggcatatg gacacggtgc tcatcgcttc cgccgcggga
     7321 tcaggagcca caacatccgc cggctggagg cgtacctcga tcgggtggag cagggtgagt
     7381 cgccgattgc gggcggggag ccgatccatg gctgggaccg cgagctcgat cgactgtttg
     7441 tcgggcttcg tcgctccgac ggtgtcgcct ccggtcctgg cgtcgccgcg ttgttggaga
     7501 cgccgggtgg tgagatcctg gtgcgggagc gggtgatcga gcttcgcgcc gggagactgg
     7561 tcgtcagtcg tcccctgctc accgatgagg ttcaccggca agtgctgagc ctcgacccgc
     7621 cgatcgggtg ggtggaggcc ccaaacccgg ataacctgtt ggcggatagg gatgctggaa
     7681 caacgtcgct ctgagattct ccgtgccctc gtcgaggagc acatacggtc gggtgagcct
     7741 gtcagctcac gggccatcgt cgaacgcgcc catctctcgg tttccacggc aaccgtcaga
     7801 cacgacctca cttccttgga ggcagagggc ttcgtcatcc agccccacac ctcggcgggc
     7861 aggatcccca ccgccg
//
LOCUS       DATITW010000085         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_324876, whole genome shotgun sequence.
ACCESSION   DATITW010000085 DATITW010000000
VERSION     DATITW010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324876"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="VLS86_01210"
     CDS             complement(<1..1332)
                     /locus_tag="VLS86_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSO49138.1"
                     /translation="MDVALVDTFAADLRGELIGGGDPEYDEARKLYNGMIDKHPALIA
                     RTVDAGDVMTAVNFGREAGLDIAIRCGGHNGPGLGSVDDGLVIDLSEMRGVRVDPEAR
                     TAQVAGGSQLGDVDHATHAFGLATPSGIFSTTGVGGLTLGGGVGNLGRTFGLSIDNLL
                     SADVVLADGSFVTTDEKREADLFWALRGGGGNFGVVTSFTFRLHPVDTVVAGPMLWPI
                     DRSTEVLSFYRDFLPSAPDELNGWFAFLTVPPAPPFPEELHLQKMCAVLWCYVGSQED
                     ADTALSPVRALAPALDGVMEMPFPVIQSAFDELYPPGLQWYWKVQLVKEISDEAVATN
                     VAFGEKLPSIFSTTHMYPVDGAAGRIDDDATAWAHRDANWVQVIAGIDPEPENADAIR
                     TWASDYWEALSPYSTGGGYVNMMMVEGEDRLKATYGEHWDRLARIKAHYDPS"
BASE COUNT          360 a          707 c          639 g          328 t
ORIGIN      
        1 gctgggatcg tagtgggcct tgatccgagc cagccgatcc cagtgttccc cgtaggtcgc
       61 cttcaaccga tcctcgccct ccaccatcat catgttcacg taaccgccgc ccgtggagta
      121 cggactgagg gcctcccagt agtcagaggc ccaggttcgg attgcatccg cgttctccgg
      181 ttccggatcg atccccgcga tcacctgaac ccagttcgcg tcccggtggg cccaggcggt
      241 cgcatcgtca tcgatgcggc cggcggcccc gtcgaccggg tacatgtggg tggtcgagaa
      301 gatcgagggc agcttctctc cgaacgccac gttggtggcc accgcctcgt cgctgatctc
      361 cttcaccagc tggaccttcc agtaccactg caacccgggt ggatacagct cgtcgaacgc
      421 actctggatc acagggaacg gcatctccat gaccccatcg agcgcggggg cgagcgcccg
      481 gaccggcgag agcgccgtgt cggcatcctc ctgcgaaccg acgtagcacc aaaggacggc
      541 gcacatcttc tggaggtgaa gctcttcggg gaagggcggc gctggcggga cggtcaggaa
      601 cgcgaaccac ccgttcagct catcgggagc cgacggcagg aagtcgcggt agaaagagag
      661 cacctccgtg gatctatcga tcggccagag catcggacca gcaaccaccg tgtcgaccgg
      721 atggagccgg aacgtgaacg aggtcacgac gccgaagttc ccgccgcctc cacgaagagc
      781 ccagaacagg tccgcctctc gcttctcatc ggtcgtgacg aaactgccgt ccgccaagac
      841 gacgtcggcc gacagaaggt tgtcgatgct caatccgaag gtccggccga ggttgccgac
      901 cccgccaccg agcgtcaggc cgccgacgcc ggtggtcgaa aagatcccgg aaggcgtggc
      961 caagccgaac gcatgcgtcg cgtggtcgac atcgccgagc tggctgccac ccgctacctg
     1021 cgccgtccgg gcctccgggt cgacccgcac cccgcgcatc tcggagaggt caatcaccaa
     1081 gccgtcgtcg acgcttccca gacctggccc gttgtgacca ccgcaccgga tcgcgatgtc
     1141 gagccctgcc tcgcgcccga agttgaccgc ggtcatcacg tcgccggcat ccaccgtgcg
     1201 ggcgatcagc gccggatgct tgtcgatcat cccgttgtag agcttgcgtg cctcgtcgta
     1261 ctccggatcc cccccgccga tcagctcgcc ccgaagatcg gcggcgaacg tgtccaccaa
     1321 tgcaacgtcc atacggactc cctcccgcga gtgcggtaca gccgattaaa gagcatggcc
     1381 ctgaacccgc gcaagccggg cggcagttca cctggatccg gaccacgcgc ccgaccaagc
     1441 ctgagtaccg tggccgcctc gtctgagtca accggtgggt gcacagcgga ctcaggcggc
     1501 cgacctgcgg gatacgcccg cgccgcctga gtcacctatg gcgggtgcgt tcggcagcgg
     1561 ccacgaaggc agacggggcg tccaaccgga cgagaagcgt gtccgatttg ctatgccggt
     1621 tcgccgaccg cgaactctcg acctcgacca acaacttcga agaggagctc cggtgcgcca
     1681 tgcgacccct ggcatcgagc ggctcctgca cgatgagaag ctctagcggc cggtcgagag
     1741 ttcgtctcca gcttcttcga gacatcgttc taagctcggt tcacgacgtg catcgcggct
     1801 ctcgggatct tgaaggagga ggccgcgttc cccgcgagcg acgcgtgaga gcgggccgca
     1861 gtcgcgaggg aacttgagca gtcccactct ccggaccggt taggaaaggc gttcgaagag
     1921 cttccgcgcg gccaggaggt cgggggtctg gtggccctcg gtgaaccact ggtagacgtc
     1981 cccgagcgcg cgcctcccct cggccttctc ggcggccgtt ccctgatctg ccca
//
LOCUS       DATITW010000086         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_284751, whole genome shotgun sequence.
ACCESSION   DATITW010000086 DATITW010000000
VERSION     DATITW010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284751"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1197
                     /locus_tag="VLS86_01215"
     CDS             109..1197
                     /locus_tag="VLS86_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSO49139.1"
                     /translation="MDPATAPLMGPSRRSFLKAMGVVTAAGLINACTSNGDANGTTLT
                     TGAAGALGAGNFPETPEWNFVFVNHVTTNPFFVPTVYGIEDASAILGTTFQWTGSEIA
                     DNGEMVNAMEAAIASGADGIGVAIVDLEAFNGPTDAALADGIPVLSYNADAPNSRLCY
                     VGQDLYGSGFEMGLRIVDLVGSGKVGLFIATPGQLNIQPRIDGAIDAIAESGADIEAL
                     DIATGADLTEELSTVEAWYLGNQDATGMFAVDAGSTQAVGQVVRDQNARDNALVGCGG
                     YDLLPETVELVADGVLDFTIDQQPYLQGFLPVVYMYLYKLSGGVITPPETNTGLVFLE
                     SASAQLFLETASRFEGDAAEQKILEPVA"
     gene            1222..2256
                     /locus_tag="VLS86_01220"
     CDS             1222..2256
                     /locus_tag="VLS86_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49140.1"
                     /translation="MTELRERDTGGDATAPPVPRSVTARVGRFLLGKPESTIAIAALI
                     LAAYFALTNDAFLTTANIRIISQYLIATAILATGQTMLLISGEIDLSMGNVYFMTPFL
                     VFFATEAGLSLPLAILIGVVGAGVVGVLNGAITIGAGIPSFIVTLAMGFIINGFTLNI
                     SDGFPKRPPSAGLASDIFGGAAFSGLAWVLVIVVLMHIVLTKTRWGVYTVAIGGNKTG
                     AREAGVPSTKIRMRNFVVAAMLAGFAGVIEGIRIGSFDPGAGVAGPRVFEAVAAAVIG
                     GTVLTGGAGTIIGAFLGALVLAILKDGLVLAGASAFTFTTVLGVAIILAMVLNAIASK
                     ARIRSQSVRL"
     gene            2253..>2496
                     /locus_tag="VLS86_01225"
     CDS             2253..>2496
                     /locus_tag="VLS86_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO49141.1"
                     /translation="MTVAASNGDVIRAESIGKRYGAVVALRDVTLRLGRGEVLGLIGD
                     NGAGKSTLIKMLSGAVVPDEGEIYVEGERVRLHGPLD"
BASE COUNT          425 a          816 c          806 g          449 t
ORIGIN      
        1 cacatgcatg acgtatgcat gcaggcgctt gcgcacaaag ggattcaggc ttatgctccg
       61 cccggccatt cggacggatg gctccgctca ccaaggagga ggaagcaaat ggacccggca
      121 actgcccctc tcatggggcc ctcgcgtcga agtttcctca aggcgatggg ggtcgtcacc
      181 gccgccggct tgatcaacgc ctgcaccagc aatggcgatg cgaacggaac cacgctcacc
      241 accggtgctg ccggggcgct tggcgcaggc aacttcccgg agacaccgga atggaacttc
      301 gtcttcgtca atcatgtgac caccaaccca ttcttcgtgc ccactgtgta cgggatcgag
      361 gacgcctctg ccatcctcgg caccaccttc caatggaccg ggtcggagat cgccgacaac
      421 ggtgagatgg tcaacgcgat ggaggcggcc atcgcctccg gcgccgatgg gatcggtgtg
      481 gcaatagtcg atctcgaggc gttcaacggc cccacggacg ccgccctcgc cgacggaatc
      541 ccggtgcttt cctacaacgc cgacgccccc aatagccgac tctgttacgt gggtcaggac
      601 ctctacggtt ccgggttcga gatgggtctg cgcatcgtcg acctggtcgg ctcgggcaag
      661 gtgggcctgt tcatcgcgac cccgggtcag ctcaacatcc agccccgcat cgatggcgca
      721 atcgacgcca tcgccgagtc cggggccgac atcgaggccc tggatatcgc caccggggcg
      781 gacctcaccg aggagctgag cacggtcgag gcctggtacc tgggaaacca ggacgccacc
      841 ggcatgttcg cagtcgacgc cggaagcact caggccgtcg gccaggtggt gcgggaccag
      901 aatgcccggg acaatgccct ggtcggctgt ggtggatacg acctcctccc ggaaacggtg
      961 gagttggtgg ccgatggcgt gctcgatttc accatcgacc agcagccgta tctgcaagga
     1021 ttcctcccgg tggtgtacat gtacctctac aaactgtcgg gcggcgtcat cacaccaccg
     1081 gagaccaaca ccggccttgt gttcctcgag tcggcctcgg cccaactctt cctcgagacg
     1141 gcgagccggt tcgagggcga tgccgcagag cagaagatcc tggagcccgt ggcctgatcc
     1201 acaaccggac aagcgggaca aatgactgaa ttgcgggagc gggacaccgg gggcgacgca
     1261 acggcgccac cggtgccccg ctccgtgacg gcccgggtcg gccgtttcct tctcgggaag
     1321 ccggagtcga ccatcgccat cgcggccctg atcctggccg cctacttcgc cctgaccaac
     1381 gatgccttcc tgaccaccgc caacatcagg atcatctccc agtacctgat cgccaccgcg
     1441 atcctggcca ccggacagac gatgcttctc atctccggcg agatcgacct ctccatgggc
     1501 aacgtctatt tcatgacgcc attcctcgtc ttcttcgcca ccgaggccgg cctctccctc
     1561 ccgctggcga tcctgattgg agtggtcggg gcaggggtgg tgggagtcct caacggtgcc
     1621 atcaccatcg gcgccggtat cccctcgttc atcgtcaccc tggccatggg attcatcatc
     1681 aacggcttca ccctcaacat ctccgacggc tttcccaagc ggccaccgtc ggcggggctc
     1741 gcctccgaca tctttggcgg tgccgcattc tccgggctgg cgtgggttct ggtgatcgtg
     1801 gtgttgatgc acatcgtgct caccaagacc cgttggggcg tgtacaccgt cgccatcggg
     1861 ggcaacaaga caggcgccag agaggcgggc gtcccttcga cgaagatcag gatgcggaac
     1921 ttcgtggtgg ccgcgatgct cgccggattc gccggtgtca tcgaagggat ccggatcggg
     1981 tcattcgatc caggggccgg agtggccggg cccagggtgt tcgaagcggt ggccgcggcg
     2041 gtgatcggcg gaaccgtcct caccggtggg gcgggcacca tcatcggcgc ctttctcggg
     2101 gcgttggtgt tggccattct caaggacggg ttggtgctcg ccggggccag cgctttcacc
     2161 ttcaccacgg tgctgggggt ggccatcatc ctggccatgg tcctcaacgc catcgcctcc
     2221 aaggcccgga tccgatccca gagcgtgcga ttatgaccgt cgccgcctcg aacggggatg
     2281 tgatccgggc agagagcatc ggcaagcgtt atggcgcggt ggtggccctg cgtgacgtga
     2341 ccctgcggct ggggcgaggt gaggtgctgg ggctgatcgg cgataacggc gccgggaagt
     2401 cgaccctgat caagatgctt tcgggggcgg tggtcccgga cgagggcgag atctacgtcg
     2461 agggcgagcg ggttcgtctc cacggtccgc tcgacg
//
LOCUS       DATITW010000087         7556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_484947, whole genome shotgun sequence.
ACCESSION   DATITW010000087 DATITW010000000
VERSION     DATITW010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484947"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="VLS86_01230"
     CDS             <1..1162
                     /locus_tag="VLS86_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49142.1"
                     /translation="TVDVAAYDRLISDLDKRQFVLHAVKSSSPTLFRTRWAMSYLAGP
                     LTRDQVSALMEGRSPLQSPSPGPVTAREEPTSTITPTVATAPTGAAGIVETYLDPAAA
                     WSGEVGADPTGTHYQAGAAATVNLLYDDTSANVSHQEVFEAVVFPLGSVLTPDSIREV
                     DHDGRDFRLSPRDGASFEAPQAGIDTKTFWTGLETDLKNHLVANRKVTVFKNPALKLY
                     SRPGESREGFESRATEMAEQTADMALVKLKERYATRIERARATLAKAENRVAELEDAA
                     QSKQQDELLSGAGDLLGAFLGGRRRSNPLGQAARRRSATQTAQNRAETASEALDDDRA
                     EVEALEQELASEIAALTEEHMAKAAEIEEVEIPLEKTDIRVAELKQVWIPVS"
     gene            complement(1191..1565)
                     /locus_tag="VLS86_01235"
     CDS             complement(1191..1565)
                     /locus_tag="VLS86_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSO49143.1"
                     /translation="MRLGDLLGDAPHVCGPDTTVLEAAIAMEGSDLGSLAVVEGMVLV
                     GLVTERDIRRSVAEGMGAETPVSSLMSGDPDTFDPDLDVWDAAAWIAESGYRHLPVMD
                     DEGTLLGVVSLRDLLKGLVDTV"
     gene            complement(1638..2624)
                     /locus_tag="VLS86_01240"
     CDS             complement(1638..2624)
                     /locus_tag="VLS86_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSO49144.1"
                     /translation="MAAAETRTDKTEKPRIRLHSKLTDERGRLTTDLVSQYLTAIGEY
                     DLLTAEQEVELAQKIEGGEEAAEKLEKGEYSGKREEMELRRFARRGAQAKDDFLTANL
                     RLVVANARRYANTSGIDFLDLIQEGNLGLIRAVEKFDWRKGFKFSTYATWWIRQAITR
                     AIADKSRTVRIPVHLHDTLAAVRAAQASLKAELGRDPRPEEIAEEAGVTPDKVELALS
                     VADTVSLEQPIGEDGAQLGDFIEDEDAADPVQMTEEMDVANSLRVSIERLPEREGRIL
                     ALRYGFYDGVPRTLEEIGDEFNLTRERIRQLEKLALCRLRHPSFGIREADLV"
     gene            complement(2868..3377)
                     /locus_tag="VLS86_01245"
     CDS             complement(2868..3377)
                     /locus_tag="VLS86_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSO49145.1"
                     /translation="MRTLLLMRHAKSDWEADYGSDHERPLNKRGTESARLMGRVLAGQ
                     ALTPDLVISSTAVRARTTAELAIEAGGWGCPLRLDRSLYDSGPETVLATAAGAPDVGT
                     LMLVGHQPTWSMLVWDLTGARVEMKTATVAMVGLDLEAWPDLEGANGSLISVLNPGTF
                     LGSEYDRPH"
     gene            complement(3387..3989)
                     /locus_tag="VLS86_01250"
     CDS             complement(3387..3989)
                     /locus_tag="VLS86_01250"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSO49146.1"
                     /translation="MQGWLDGPSLEARPMRRGDLAPSVEVLDTDGAPVSLESIWRERP
                     GLLLLWRHLGCGCGLERAERLMTEYQGYIDSGLNVAIVAPGEPERVAAYRAKYKVPAP
                     MLADQDYSAHMAFGLGHWSVEQAVYDAPDEFCELREDTGIEFQADRRSQGRPLVDDPW
                     MQSGEFVVAPDGVIRVAYLYNYCADYPDPRVFTTAGRLLS"
     gene            4167..6146
                     /locus_tag="VLS86_01255"
     CDS             4167..6146
                     /locus_tag="VLS86_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSO49147.1"
                     /translation="MPNRLADATSPYLLQHQDNPVDWHEWGDTAFAEARERDVPVLLS
                     VGYSACHWCHVMAHESFEDPETAAVMNRLYVNIKVDREERPDVDSIYMEAVQTMTGRG
                     GWPMTVWLTPDGRPFYAGTYFPDEDRHGMPAFRSVMRAVSDAWDTKREEVLGQSDRLL
                     DAISRNIPAAEGVPDAGTLQTAYASLASSFDPINGGFGSAPKFPQQPVLEFLLRAHAE
                     DWAPRAAHMVKVTLAEMADGGIHDQLAGGFARYSVDDQWLVPHFEKMLYDNAQLARLY
                     LWAGIELDEPRFVEVARATLGYLVTDLHDPEGGFHSAEDADSEGVEGKFYVWTTEELE
                     DVLGSADAVRAAAFFGATPHGNFEGSNILYRPTSEDWTDEIESIRIRLRAHRAKRVRP
                     GLDDKVIASWNGLAIRAFAEAGAALGDESYLDIARTTSRFVLDRMVVDGKLRRSWRKG
                     RVGVAGFLEDHAAIGLGLFALYAATGEIDWYLAARDLTLGIPQQFADPDGGFFDTALD
                     GESLIKRPKSQADNPLPSGNGMAAEALMILSAYTGEAGHRELAESAIRSAGLLMERYP
                     SMVGHHLAVLHSSLDSKELAVVGPDWKELSRVYWERFRPEVVIAVSDDGSDPVPLLTG
                     RDGSGTTQAYLCRGHVCELPTPDREKLRDQLDSGL"
     gene            complement(6147..6935)
                     /locus_tag="VLS86_01260"
     CDS             complement(6147..6935)
                     /locus_tag="VLS86_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSO49148.1"
                     /translation="MFDELGDGVFRRRYESMDLNVGVVVSEQGILVVDTRCTHVEADE
                     LLADIRTLSPLPVRWVVDTHWHWDHTFGNSRFTGAEIWGHELCRRALEERGESMKHAA
                     AEWLPDLGDDIESVVIAAPSRSFADTASIDLGSRTALLSYHGLGHTDSDIAISVEGED
                     VLFLGDLVEESGPPHFDDGYPIHWPGTLRSALHPGKAVIVPGHGDTMSWAEADEQLEE
                     LESVALLARECVEAGAPVSQAAAQGPYPVEVMISALTRAVEIGP"
     gene            complement(6969..>7556)
                     /locus_tag="VLS86_01265"
     CDS             complement(6969..>7556)
                     /locus_tag="VLS86_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HSO49149.1"
                     /translation="LIGAMIVLAEWVGDDASVARVIAVPLHLVNTLFLLAALTLTAYW
                     LSGGRSVSPRTHPVVWRWVIFGGLSIVLLAATGAVTALADTLFPKSGTGSGATTQEHL
                     LTDLRMIHPVLAVIAASIGWWASGRVAGSRSAAGRALPVLVGLMLITGSLNIVLGVPV
                     WMQLVHLFLADALWIAYVLASAEALAAPVEARQSA"
BASE COUNT         1347 a         2604 c         2395 g         1210 t
ORIGIN      
        1 gactgtcgac gtggctgcct acgaccggct gatctcggac ctcgacaagc gccaattcgt
       61 cctccatgcc gtcaagtcct cctcaccgac cctgttccgg acgagatggg cgatgtccta
      121 cctcgccggc ccgctgaccc gcgaccaggt gtccgccctc atggaaggcc gatccccgct
      181 ccagtcccca tcaccgggtc cggtgacggc cagggaggag ccgacgtcga ccatcacgcc
      241 caccgtcgcc accgcgccga ccggcgcagc cggcatcgtg gagacgtatc tcgacccggc
      301 cgctgcatgg tcgggtgagg tcggcgccga tcccaccggg actcactacc aggcaggagc
      361 ggcggccacg gtcaacctgc tctacgacga caccagcgcc aacgtcagcc accaggaggt
      421 cttcgaggcc gttgtcttcc ccctcggctc cgtcctcacc cccgattcga tccgggaggt
      481 cgatcatgac ggacgcgact ttcggctcag cccgcgggac ggagcatcgt tcgaggctcc
      541 ccaggccggc atcgacacca agaccttctg gaccgggctc gagaccgacc tgaagaacca
      601 cctggtggcc aacaggaagg tgaccgtctt caagaacccg gcgctgaagc tctactcacg
      661 accgggtgag tcccgcgagg gcttcgagag cagagccacg gagatggccg agcagaccgc
      721 ggacatggcc ttggtgaagc tgaaggagcg ttatgcgacc cgaatcgagc gggcacgcgc
      781 cactctcgcc aaggcggaga accgggtcgc cgagctcgag gacgcggccc aatccaagca
      841 acaagacgag ctgctctccg gcgccggcga cctgctcggg gcgtttctcg gtggccgcag
      901 acgctccaac ccgctggggc aggccgccag gaggagatcc gccactcaga ccgcccagaa
      961 ccgggccgag actgcgagcg aggcgctcga cgacgaccgc gcagaggtcg aagcgctgga
     1021 acaggagctg gcctcggaga tcgccgccct caccgaggag cacatggcca aggccgccga
     1081 gatcgaagag gtcgagatcc ccctggagaa gaccgacatc agggtggccg agctgaagca
     1141 ggtgtggatc cccgtctcct gaccgagact ccattggatc cctggcggga tcagaccgtg
     1201 tccaccaggc ctttgagcag gtctcggagc gagaccacgc cgagaagtgt cccctcgtcg
     1261 tccatgaccg ggagatgccg gtatcccgat tcggcgatcc aggcggccgc gtcccagaca
     1321 tcgaggtccg gatcgaaggt atcgggatca ccgctcatca ggctcgacac cggggtctcg
     1381 gcgcccattc cctccgccac cgacctgcgg atgtcccgct cggtaaccag cccgaccagg
     1441 accattccct cgaccaccgc cagtgacccg agatcggagc cctccatggc gatcgcagcc
     1501 tccagcactg tggtatcagg gccacaaacg tgtggcgcat cgccgaggag gtcacccagt
     1561 ctcatcaggt gacaccatag atagaaagaa gacccaccgt ttccggtggg ccctcttcga
     1621 cagatgctga gatccgctca gaccaggtcg gcctctcgga tcccgaagga cgggtggcgg
     1681 agacggcaca gcgcgagctt ctccaattgc cggatgcgct cgcgggtgag gttgaactcg
     1741 tccccgatct cctcgagggt gcgggggacg ccgtcgtaga agccataacg caaggcgagg
     1801 atccgccctt cgcgctcggg cagtctctcg atcgagaccc ggagcgagtt ggccacatcc
     1861 atctcctcgg tcatctggac cgggtcggcc gcgtcctcgt cctcgatgaa gtcaccgagt
     1921 tgagcgccgt cctcaccgat aggctgctcc agggacacgg tgtctgcaac gctgagtgcc
     1981 agctccacct tgtccggggt gacaccggcc tcctcggcga tctcctcggg acgtgggtca
     2041 cgacccagct cggctttgag cgaggcctgg gcagctcgaa ccgcggccag cgtgtcgtgg
     2101 aggtgcaccg ggatgcgcac ggtccgcgac ttgtcggcga tggcgcgggt gatcgcctgg
     2161 cggatccacc atgtcgcgta ggtggagaac ttgaacccct tgcgccagtc gaacttctcg
     2221 acggcgcgga tcaggccgag atttccctcc tggatcaggt ccaggaaatc gatgcccgag
     2281 gtgttggcgt agcgacgagc gttggccacc acgagacgga ggttggccgt gaggaagtcg
     2341 tccttcgcct gcgccccacg ccgggcgaat cgacgaagct ccatctcctc gcgcttcccg
     2401 ctgtactcgc ccttctccag cttctcggcg gcctcttcgc caccttcgat cttctgggcg
     2461 agctccactt cctgctcggc cgtgagaagg tcgtactcgc cgatcgcggt cagatactga
     2521 gaaaccagat ccgtggtgag acggccgcgc tcgtccgtca gcttcgagtg aagtcggatg
     2581 cgcggcttct cggttttgtc cgtacgcgtt tcagcagcag ccatctagat ctagtcctcc
     2641 tggagccctg tgggcggggg atggggcatc cccgaacgtc caactatgac acaagagcgg
     2701 tggtttgtga acccccctct ttgcgacagt gaactggcga ttcggcgacc cgaacctcga
     2761 ttctaccccg atatcgacgc aaccacccac gttctcgtgg tcggcctcac cagtaaaaac
     2821 gggcttctca ccgggtttgt tccccgattc ttcgccgttg tgaccgctca gtgggggcgg
     2881 tcgtactccg acccgaggaa cgtccccggg ttgagcacag agatcagaga gccgttggcg
     2941 ccttcgagat cgggccaggc ctcgaggtcg agaccgacca tcgcgaccgt tgccgtcttc
     3001 atctccaccc tggcgccggt caggtcccag accaacatcg accacgtggg ctggtgaccc
     3061 accagcatca gggttccgac atcgggagca ccagccgcag tggcgagcac ggtctctggt
     3121 cccgaatcgt agagcgagcg atcgagtcgg agcgggcagc cccacccacc ggcctcgatg
     3181 gcaagttccg ccgtggtccg cgcccgcacc gccgtcgagc tgatcacgag atcgggtgtg
     3241 agcgcctgcc cggccagcac ccggcccatc agtcgggcgc tctcagtccc ccgcttgttg
     3301 agcggccgct cgtggtccga gccatagtcg gcctcccagt ccgacttcgc gtgacgcatg
     3361 aggaggagag ttcgcaccct gaaccgtcag ctgaggagac ggccggcggt ggtgaagacc
     3421 ctggggtccg ggtagtcggc gcagtagttg tagagatagg cgacccggat caccccatca
     3481 ggcgccacca cgaactcgcc cgattgcatc cacgggtcgt ccaccagcgg gcggccctgg
     3541 gatcgccggt cggcctggaa ctcgattccc gtgtcctcac gaagctcgca gaactcgtcg
     3601 ggagcgtcat acacagcctg ctccaccgac cagtgcccga gaccaaacgc catgtgggcc
     3661 gagtagtcct ggtcggcgag catcggggcc gggaccttgt atttggcgcg gtaggcggcg
     3721 acccgctcgg gctcccccgg cgccacgatc gccacgttga ggccactgtc gatgtacccc
     3781 tggtattcgg tcatcagccg ctccgcccgc tccaaaccgc atccgcaacc gagatgacgc
     3841 cagagaagca gcagacccgg ccgttcccgc cagatcgact ccagcgacac cggggccccg
     3901 tccgtgtcga gcacttcgac gctgggagcg agatcccccc tgcgcatcgg tcgggcctcg
     3961 aggctgggcc cgtcaagcca cccctgcatc catctccgct cagccgagag gtacagcgct
     4021 tgaaaatccc tgtcgttcat cgtcttcccc cagattccgc cgggacgacc ctaatccggc
     4081 attgcacgag gcgatcgccg gcctgacgcc aggccagcat cgggagcagg ctgagttgat
     4141 cacgacatgg actacgttca ccgacgatgc ccaaccgact cgccgacgcc acgtcgccct
     4201 atctcctcca gcaccaggac aacccggtcg actggcacga gtggggtgac accgccttcg
     4261 ccgaagccag ggagcgtgac gtcccggtgc ttctcagcgt cggctattcg gcctgtcatt
     4321 ggtgccatgt gatggcacac gagtcgttcg aggacccgga gactgcggct gtgatgaacc
     4381 gtctctacgt caacatcaag gtcgaccgtg aggaacggcc cgacgtcgac agcatctaca
     4441 tggaggcggt gcagacgatg accggccggg gagggtggcc gatgacggtg tggctcaccc
     4501 ccgatgggcg gcccttctat gccggcacct actttcccga cgaggaccgt cacgggatgc
     4561 cggccttccg cagcgtgatg agagcggtct ccgacgcatg ggacaccaag cgcgaggagg
     4621 tcctgggcca gtccgatcgg ctgctggacg ccatcagcag gaacatcccg gccgcagagg
     4681 gggtgcccga tgccgggaca ttgcagactg cttacgcctc cctggcctcg agcttcgacc
     4741 cgatcaacgg cggcttcggg agcgctccca agttccccca acaacccgtc ctcgagttcc
     4801 tgctccgggc ccacgccgag gactgggcgc cacgcgccgc ccacatggtc aaggtcacgc
     4861 tcgccgagat ggccgacggg ggcatccacg accagttggc cggcgggttc gctcgctact
     4921 cggtcgacga ccaatggctg gtcccccatt tcgagaagat gctgtacgac aacgcccaac
     4981 tggctcgtct ctatctctgg gccggcatcg agctcgacga gccgaggttc gtcgaagtcg
     5041 cccgcgccac cctgggctat ctggtcacgg atctgcatga tcccgaaggt ggcttccact
     5101 cggcggaaga cgccgacagc gaaggtgtcg aggggaagtt ctacgtgtgg accaccgaag
     5161 agctcgagga cgtcctgggg agcgcggacg cggtcagggc cgcagctttc ttcggggcaa
     5221 ccccccacgg caacttcgaa ggctccaaca tcctctaccg gccgaccagc gaggactgga
     5281 ccgatgagat cgagtcgatc cgcatccgac tccgcgccca ccgggcaaag cgggtcagac
     5341 ccggtctcga cgacaaggtg atcgcttcct ggaacggtct cgccatccga gcgttcgccg
     5401 aagccggggc ggcgttgggc gacgaaagct acctcgacat cgcccgcacc acgtccaggt
     5461 tcgtgctgga ccggatggtc gtcgacggga agctgcgtcg ctcgtggcga aagggaaggg
     5521 tgggcgtggc cggtttcctc gaggatcacg ccgccatcgg cctgggcctg ttcgccctgt
     5581 acgcggccac cggggagatc gactggtacc tcgccgcccg ggacctgacc ctcggcattc
     5641 cccagcagtt cgccgaccct gacggcggct tcttcgacac cgctctcgat ggcgagagcc
     5701 tgatcaagcg ccccaagagc caggcggaca accccctgcc ctcgggaaat gggatggccg
     5761 ccgaggcact gatgatcctg tccgcctaca ccggagaggc cgggcaccgg gagctggctg
     5821 agagcgccat tcgctccgcc ggtctcctca tggagcgcta cccgtcgatg gtcggccacc
     5881 acctcgccgt gctccactca tcgctcgaca gcaaggaatt ggccgtcgtc gggccggact
     5941 ggaaggaact gtcccgtgtg tactgggaga gattcagacc cgaggtcgtg atcgcggttt
     6001 ctgacgacgg gtcggatccg gtgccgctgt tgaccgggcg agacggctca gggacaacac
     6061 aggcctatct gtgccgcggc cacgtctgcg agctcccgac accggacagg gagaagctga
     6121 gagaccagct cgactctgga ctgtgatcag ggcccgatct ccacggctcg ggtgagggcg
     6181 gagatcatca cctccaccgg atacgggccc tgtgctgcgg cctggctcac cggcgccccc
     6241 gcctcgacac attcccgggc gaggagggca accgattcca gctcttcgag ctgctcgtct
     6301 gcctccgccc agctcatggt gtcgccatgc ccggggacga tgaccgcctt gccggggtgg
     6361 agagccgagc gcagggtccc cggccagtgg atcgggtagc cgtcatcgaa atggggaggc
     6421 ccgctctcct cgaccagatc tcccaggaag agaacgtctt ccccttccac ggaaatcgcg
     6481 atgtcggagt cggtgtggcc cagaccgtga tagctgagca gggccgtcct tgacccgagg
     6541 tcgatgctgg cggtgtcggc gaagctccgg gacggcgccg cgatcaccac cgattcgatg
     6601 tcgtcgccga gatcgggcag ccattccgcc gcggcatgct tcatcgactc tcccctctcc
     6661 tccaacgccc tgcgacagag ttcgtgtccc cagatctcgg cgccggtgaa gcgagagttg
     6721 ccaaatgtgt gatcccaatg ccagtgggtg tcgaccaccc acctgaccgg gagaggggag
     6781 agagtgcgga tgtcggccaa cagctcgtcc gcctcgacgt gggtgcagcg agtgtcgacg
     6841 accaggatcc cctgctcgga gacgacgact ccgacgttga ggtccatcga ctcgtacctt
     6901 ctccggaaca ccccgtcgcc gagctcgtcg aacacgttga tgaccctagg accctgcgtc
     6961 cccgatggct atgcgctctg cctggcctcg accggcgcgg ccaaggcctc ggcggaggcg
     7021 agcacgtagg cgatccacag cgcgtcggcc aggaacaggt ggaccagctg catccagacc
     7081 gggacgccga gcacgatgtt gagcgacccg gtgatgagca tcaggccgac caggacgggc
     7141 agggcacggc ctgcggccga tctgctgccc gcgactcgac ccgaggccca ccatccaatc
     7201 gacgccgcga tcaccgccaa caccggatgg atcatccgca ggtccgtcag cagatgctcc
     7261 tgcgtggttg ccccactccc ggtccccgac ttggggaaca acgtgtcagc gagcgcggtc
     7321 accgccccgg ttgccgccaa cagcacgatc gacaacccgc cgaagatcac ccatcgccag
     7381 accacggggt gggtgcgagg cgacaccgac cggccaccgg acagccagta ggcggtcaga
     7441 gtgagcgcgg ccagcagaaa caacgtgttg accaggtgca atggcaccgc gatgaccctg
     7501 gcgaccgagg cgtcgtcccc gacccattcg gcgagcacga tcatggcccc gatgag
//
LOCUS       DATITW010000088         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_484977, whole genome shotgun sequence.
ACCESSION   DATITW010000088 DATITW010000000
VERSION     DATITW010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484977"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VLS86_01270"
     CDS             complement(<1..413)
                     /locus_tag="VLS86_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49150.1"
                     /translation="MEDQTERGSSWPLYLVGALFVVGALILGAWNLELPYLAFSSGPV
                     SDAADAIVVSEVETYPPQGELLMLTVVSQDINLFEAFIAGIDPTIDLVRAEAYRQPEE
                     SDEDYRKRVLQQMDDSQTRAITVALDYLGYDMVPT"
     gene            495..1076
                     /locus_tag="VLS86_01275"
     CDS             495..1076
                     /locus_tag="VLS86_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HSO49151.1"
                     /translation="MTSTVISQRVLPRSAVVSVLLVVGAAGLTALAAQWEIHLAFTPV
                     PITGQTFAVLLTGAALGSRLGAAGQTLYVAAGAAGLPVYAGGAAGWDQAQAVGTTGYL
                     IGFIVAAGLVGYMAERRQDRTFPTMFTAFILGSFVIYGFGVTGLILNFGMSLVEAIVA
                     GVVPFLLGDLIKAACAGLLLPGAWKLVGEHPRA"
     gene            complement(1083..1910)
                     /locus_tag="VLS86_01280"
     CDS             complement(1083..1910)
                     /locus_tag="VLS86_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009157175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO49152.1"
                     /translation="MSGKLLERRAERSGPKSYEHLLVVDDLHLAFKGVKAIDGVSFHV
                     DDGELFAIIGPNGAGKTSLFNSINQVYNPQEGDILWKGESLMGLKPDRTAALGIARTF
                     QNIALFPHMTVIENLLTGRHIRMRSSWLAGSVWLGKAKREEIENREIVEDIIDFLEIE
                     QWRKYPVGLLPYGIQKRVELGRALAMDPELLMLDEPVAGMNLEETEDMARFILDVQDE
                     LGIAIILVEHDMGLVMDIADRVLVVDFGKAIATGVPAEVQQNPNVIKAYLGEEVETV"
     gene            complement(1907..>2302)
                     /locus_tag="VLS86_01285"
     CDS             complement(1907..>2302)
                     /locus_tag="VLS86_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013808740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO49153.1"
                     /translation="RSGQYAGTLSGGEQQMLATGRGLMSEPKLLILDEPSLGLAPLLV
                     QEVRDIIVEINNQGTSVLLIEQNAKMALSISHHGYIMETGKMVMDGDPARLLRDDDVK
                     EFYLGLHATGEQKSFRDVKHYKRRKRWLS"
BASE COUNT          383 a          756 c          709 g          454 t
ORIGIN      
        1 tcggtaggga ccatgtcata tcccaggtag tcgagcgcga ctgttatcgc ccgggtctgc
       61 gagtcgtcca tttgctgcag gacacgcttt ctgtagtcct cgtcggactc ctccggctgg
      121 cgataggcct cggcccgcac caggtcgatg gtggggtcga tcccggcgat gaaagcctcg
      181 aagagattga tgtcctggct gaccacggtg agcatcagca actcaccttg gggcgggtag
      241 gtctcgactt cgctgacgac gatcgcatcc gcggcgtctg agaccggccc cgaggagaag
      301 gcgagatagg gcagttccag gttccaggcg cccaggatca gggcgcccac gacgaacagg
      361 gccccaacca ggtacagggg ccaggaagag ccgcgttcgg tctggtcttc cacaggcaaa
      421 agagtaacgg tgccctccgg gtaggggtct ctacacctaa ggttgcacgc cgagtggttt
      481 cgaggagcta aagaatgaca tcgaccgtca tctcccaacg ggtcttgccc agatcggccg
      541 tcgtctcggt gctgttggtg gtcggcgccg ccgggctcac cgccctggcc gcccagtggg
      601 agattcacct cgcgttcacc ccggtcccga taacgggtca gacctttgcc gtgctcctca
      661 ccggcgccgc cctcgggtcc aggctcgggg cggccgggca gactctgtat gtggccgccg
      721 gcgcagccgg cttgccggtg tacgcgggtg gggccgccgg ctgggaccag gcgcaggcgg
      781 taggaaccac cggttatctg atcggcttca tagtggcggc aggactggtc gggtacatgg
      841 ccgagcggcg ccaggaccgg acctttccca ccatgttcac cgccttcatc ctcggatctt
      901 tcgtcatcta cggattcggg gtgaccgggc tcatcttgaa ctttggcatg tcactcgtag
      961 aggcgatcgt cgccggggtc gtcccgttct tgttggggga cctgatcaag gctgcatgcg
     1021 ccggtttgct cctccccggc gcctggaagc tggtcggaga gcacccccgc gcctgaccgc
     1081 actcagaccg tctccacctc ttcaccgagg taggccttga tcacgttcgg gttctgttgg
     1141 acctcggctg gcacgccggt tgcgatcgcc ttcccgaagt cgaccaccaa cacccggtcg
     1201 gcgatgtcca tcaccagccc catgtcgtgc tcgacgagga tgatggcgat ccccaactcg
     1261 tcctgaacgt cgaggatgaa cctggccatg tcctcggtct cctcgaggtt catccctgca
     1321 acgggctcgt cgagcatcag caactcgggg tccatggcca gggcccggcc cagctccacc
     1381 cgcttctgga ttccgtaggg gagaagcccg accgggtact tgcgccactg ctcgatctcc
     1441 aggaagtcga tgatgtcctc gactatctcc cggttctcaa tctcctcccg cttcgccttg
     1501 cccagccaca cactccccgc cagccacgag ctgcgcatcc tgatatggcg accggtgagc
     1561 aggttctcga tcacggtcat gtgcgggaac agggcgatgt tctgaaatgt cctggcgatc
     1621 ccgagcgccg cggtccggtc cggcttgaga cccatcaggc tctcgccctt ccacaggatg
     1681 tcaccctcct gggggttgta gacctggttg atcgagttga acagggaagt cttccccgct
     1741 ccgttggggc cgatgatcgc aaacaactcg ccgtcgtcga catggaacga gacgccatcg
     1801 atcgccttga cccccttgaa ggcgagatgc agatcgtcaa ctacgagcag atgctcgtag
     1861 ctcttcggcc ccgaccgttc ggcccgccgc tcgaggagct tgccgctcat gacagccacc
     1921 gcttgcgacg cttgtagtgc ttgacgtcac ggaacgactt ctgctcaccg gtggcgtgca
     1981 aaccgagata gaactccttc acgtcatcgt ctctgagcag ccgcgccggg tcgccgtcca
     2041 tgaccatctt cccggtctcc atgatgtatc cgtgatggga gatggacagg gccatcttgg
     2101 cgttctgctc gatgagcagc accgacgtcc cctggttgtt gatctcgacg atgatgtctc
     2161 gcacctcctg aaccagcaag ggtgccagac ccagcgaagg ctcgtcgagg atcagcagct
     2221 tcggctcgct catcaggccg cggccggtgg cgagcatctg ctgctcgccg cccgacaggg
     2281 tgccggcata ctggccggag cg
//
LOCUS       DATITW010000089         1885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_725456, whole genome shotgun sequence.
ACCESSION   DATITW010000089 DATITW010000000
VERSION     DATITW010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725456"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VLS86_01290"
     CDS             <1..698
                     /locus_tag="VLS86_01290"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HSO49154.1"
                     /translation="MLASVGLTASDLQCPPSRPLSETAWRRNQAAGDDAPQRIWHNCS
                     GKHAAWLRASRARGWPIEGYLDPEHPLQRRIAGFVSELGEYPATPSGVDGCGAPVHRT
                     TTRAMALLFARLATLPDLREVFAAMHRFPALVSGTGNGDAAVATAINAAAKRGAAGCA
                     GVAIENRLGVAVKAWDGDGSVADVAMVSTLESLGVLSSYATAQLASIARPAVLGGESQ
                     VGEVEPRVELQWT"
     gene            695..1159
                     /locus_tag="VLS86_01295"
     CDS             695..1159
                     /locus_tag="VLS86_01295"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HSO49155.1"
                     /translation="MSADRGAGPPGLGAAMRDVLERRIETWLADPGSSVEYAEQLEGR
                     WAVRMRQEARDATTVWFTIGERSLEYEAYLLPVGEGTTELFAQALHRNMRSWRVFFAL
                     DGEGALVLRGRLSGWSVTDLELDLALGEVYDTIEVSFRPLLRALLENRENSG"
     gene            complement(1430..>1885)
                     /locus_tag="VLS86_01300"
     CDS             complement(1430..>1885)
                     /locus_tag="VLS86_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49156.1"
                     /translation="SGWDQIGENVGRGQSPTSLHKAFMASSGHKKNIVGEYNDVGIGT
                     GSSEGYLYVTVVFMKKGSTASPTTTEAPAPTTTTTTVAGAPSVKSDPAPADPEPEPEK
                     TNTTTTTLATTTTTVPPTTTTSLVVPPDKAVTPGQSCLEANRYYQLCHS"
BASE COUNT          292 a          577 c          674 g          342 t
ORIGIN      
        1 cgatgctcgc ctcggtaggg ctcaccgcat ccgatctgca atgcccccca agccgtccgc
       61 tgtcggagac ggcctggcgg cgaaatcaag ccgcgggtga cgacgccccg cagcggatct
      121 ggcacaactg ctcgggcaag catgccgcct ggctccgagc ctcccgagcc cgcgggtggc
      181 cgatcgaggg ctatctcgac cccgagcacc ccctgcagag gaggatcgcc gggttcgtct
      241 ccgaattggg cgagtatccg gcgacaccct cgggggtcga cggttgcggg gcgccggtgc
      301 atcgcaccac gacccgggcc atggcactgc tcttcgcccg actggcgacc ctgcccgacc
      361 tgagagaggt gttcgccgcc atgcatcgat tcccggctct ggtctccggc accggcaacg
      421 gggacgcggc tgtggccact gccatcaacg cagccgccaa gcggggggcg gcaggctgtg
      481 ccggcgtggc gatcgagaat cggctcgggg tggcggtcaa ggcctgggac ggtgacggat
      541 cggtcgccga cgtggcgatg gtgagcaccc tcgagtccct cggcgtgctc tcgtcctacg
      601 ccacggccca gctggcttcg atagcacggc cggcggtgct cggtggggag agccaggtag
      661 gggaggtcga gccgagggtg gagctgcaat ggacgtgagc gcggatcggg gtgccggccc
      721 cccgggcctc ggggcggcaa tgagagatgt cctggagcgt cggatcgaga cctggctcgc
      781 cgatccgggc tcgagcgtcg agtacgcaga gcaattggag ggccgctggg cggtccggat
      841 gcgtcaggag gcccgggatg ccaccacggt ctggttcacg atcggggagc gaagcctcga
      901 gtacgaggcc tacctgctgc cggtgggcga gggcacgacc gaactcttcg ctcaggccct
      961 gcatcggaac atgaggtcgt ggcgagtctt cttcgccctc gacggcgagg gcgcgttggt
     1021 gcttcggggt cggttgtcgg gctggtcggt gaccgatctc gagttggatc tggccctggg
     1081 cgaggtctac gacaccatcg aggtcagctt ccggccattg ctacgcgcct tgctcgagaa
     1141 tcgcgaaaat tcgggttaga aacttgtgaa cacatccatg tggacaagcc ctgtggataa
     1201 ccccgatttc tgttggtaac ccgccccgga accgtttcga ccctcccccc aacggtcgat
     1261 gacgttggtt gacgatagtc aaagctacaa acgcacacat tgattagccg atagcgaact
     1321 cgtacattcg gcgctgcgaa cgcccgagag gcagaccaga gcaccgtccg ttcggggacg
     1381 tgcggaggag gctctggaaa gtcactcggg cgttggcgcg cccgacaact cagctgtgac
     1441 agagctggta gtagcggttt gcctcgagac aggactggcc gggggtgact gccttgtcgg
     1501 gggggacgac gagactggtg gtcgttgtcg gcgggacggt tgtcgtcgtg gtcgccaacg
     1561 ttgtcgtggt ggtgttcgtc ttctcgggct cgggctcggg gtcggcgggg gccggatcgg
     1621 atttcaccga gggcgccccg gcgaccgtgg tggtggtcgt ggtgggcgca ggagcttcgg
     1681 ttgtggtcgg gcttgccgtc gagcccttct tcatgaagac gactgtcacg tagaggtatc
     1741 cctcactgct tccggtgcca atccccacgt cgttgtactc gccgacgatg ttcttcttgt
     1801 ggcccgacga agccatgaac gccttgtgaa gtgaggtcgg gctctgcccg cgaccgacgt
     1861 tctcgccgat ctggtcccag ccgct
//
LOCUS       DATITW010000090         3413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_404968, whole genome shotgun sequence.
ACCESSION   DATITW010000090 DATITW010000000
VERSION     DATITW010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404968"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VLS86_01305"
     CDS             <1..841
                     /locus_tag="VLS86_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49157.1"
                     /translation="VEAVEEPLSEEPVEEPAEAAVEEPLSEEPVEAPAEEPVAEEPMT
                     PAAEEPAGPDPEVETETEPAAAAVATAVEAKPVVSGNGSGNGKPASVVTARPVVPPKG
                     VPEGVRTQRLLTVVKARAIQTVKTEPTDKVNTWPHLMLMEFGALLAITGLLVIMSVII
                     HSPLLDPANFNATPNPSKAPWYFLGLQELLSYFDPQIAGVMVPTITGMIGFMMVPFVD
                     KNPSTRPSDRKFAIALYSLFLAGAATLTIIGVLFRGRGFNFSYPWKDGVFFDDLKDWV
                     HFE"
     gene            838..1740
                     /locus_tag="VLS86_01310"
     CDS             838..1740
                     /locus_tag="VLS86_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HSO49158.1"
                     /translation="MTLTTLALIAVAVVAILGAAGAFAVAYRRSQSVEKPDPLAGVSA
                     ETRAADRPVTTIWLPQSVDEAGDVEATEAETAEPGVEEAPVPVEQGDLAAVHIVETQR
                     IVEVSPEEGGVSRRQFFNRAIAGSFGAFLALQGVFYLGFFWPKLSGGFGADINAGPVG
                     DLRTQVFTSDGAVLPVFIPEARAYLVPAPAQLSEQFEGLSVAAEGLMALFQRCVHLGC
                     RVPWCAPSQGFECPCHGSKYNSIGEYFGGPAPRNLDRFVVEIRGNDFWIRTGTIIQTP
                     RAPERTVEYPQGPSCIGAVAVAPQ"
     gene            1737..>3413
                     /locus_tag="VLS86_01315"
     CDS             1737..>3413
                     /locus_tag="VLS86_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSO49159.1"
                     /translation="MTANIITVLAVVAGIAWLGVLFVSAIRNRGSEEIAPNLRPGITD
                     EEMETRRLEGGQKAAIVFSAFLAISLPLYFLGEPARQQGFVEEFAASSITRGEHIVTE
                     FACFSCHGPEGSGGVASYVEKRSGVTVSWAAPSLNDIFFRFDEDEVNYWVTFGRGNTP
                     MPPWGLPGGGPLNDEQVVDVVNYLKTIQVSQAENLVDVEPGINNALAALADADATVEA
                     AILKQEQVVAQINSAPADVAIVVPLAESARSVLDNAVDGLDTDADGLSDSAETELSAI
                     SQQAVDGFQIMDPVTLDPAVPDAELAAEALAALQAAAETDPIVITSIDAIEEALEQGE
                     VDPVVGLSPASLTALDETRAAAETAGIEVPATVENLADAEALVAALEEAAAAADPVEA
                     AAGLVTEATEAVEDGSDPDGDGLSSGAEEAVSTQVAAAISATTPPQVTLITLDPTNPA
                     SVGGVPDAATASQFVGNLESLVTTLSVTASNQDALRTTEEEGLAFLRESLEVKAYEID
                     FDGVAEAMGSSVEEAQRAVGLFNSTCARCHTAGYSAGVPFTQEAGSGGFGP"
BASE COUNT          537 a         1176 c         1143 g          557 t
ORIGIN      
        1 ggtcgaggcc gtggaggagc ccctgtccga ggagcccgtc gaggagcccg ccgaggcagc
       61 agtggaggag cccctgtccg aggagcccgt cgaggcgccc gccgaggagc ccgtggccga
      121 ggagccgatg actccggccg ccgaggagcc cgccggcccc gatcccgagg tggagaccga
      181 gaccgagccc gccgcggccg cggtggccac cgcagtcgag gccaagcccg tcgtgtccgg
      241 caacggctcc gggaacggca agcccgcctc ggtggtcacg gcgcggcccg tagtcccgcc
      301 caagggagtc cccgaggggg tccgcaccca gcgactcctg accgtggtca aggcgcgggc
      361 gatccagacg gtgaagaccg agccgacgga caaggtcaac acctggcccc atctcatgct
      421 gatggaattc ggcgcccttc tcgccatcac cgggctcctg gtgatcatgt cggtgatcat
      481 ccactccccg ttgctcgacc cggccaactt caacgcgacc ccgaacccgt ccaaggcccc
      541 ctggtacttc ctcgggcttc aggagctgct gtcgtacttc gaccctcaga tcgccggggt
      601 catggtcccg accatcaccg ggatgatcgg gttcatgatg gtccccttcg tcgacaagaa
      661 cccttccacc cggcccagtg atcgcaagtt cgccatcgcc ctctactccc tgttcctggc
      721 cggggcggcc accctgacca tcatcggtgt cctgttccgt ggccgcgggt tcaacttctc
      781 ctacccgtgg aaggacggtg tgttcttcga cgacctcaag gactgggtgc atttcgaatg
      841 accctgacca cccttgccct gatcgcggtc gccgtggtgg ccatcctcgg cgcggcaggc
      901 gccttcgccg tcgcctatcg gcgttctcag agtgtcgaga agccggaccc actggccggg
      961 gtgtccgccg agacgagggc cgccgaccgc ccggtgacca ccatctggct gccgcagtcg
     1021 gtcgatgaag ccggagacgt cgaagcgacc gaggccgaga ccgccgagcc gggggtggag
     1081 gaagccccgg tccctgtgga gcagggtgac ctggcggcag tccacatcgt ggagacccag
     1141 cgcatcgtcg aggtgtctcc ggaggaaggc ggggtgagtc gtcgtcaatt cttcaatcgg
     1201 gccatcgccg gcagcttcgg tgctttcctc gccctccagg gcgtgttcta cctcggcttc
     1261 ttctggccca agctgagcgg tggctttggc gccgacatca atgccggccc ggtgggcgat
     1321 ctcaggaccc aggtgttcac ctccgacggc gcggtgctgc cggtgttcat cccggaggcc
     1381 agggcctacc tggtcccggc accggcccag ctctccgagc agttcgaggg gctcagcgtg
     1441 gctgccgagg gtctgatggc cctcttccag cgctgcgtcc atctcgggtg ccgggtcccg
     1501 tggtgcgccc cctcccaggg attcgagtgc ccgtgtcatg gctccaagta caactcgatc
     1561 ggggagtact tcgggggccc cgctccgaga aacctcgacc ggttcgtggt cgagatccgg
     1621 ggcaacgact tctggatccg gaccgggacc atcatccaga ccccccgcgc cccggagcgc
     1681 acggtcgagt acccacaggg accgagctgc atcggcgcgg tggccgtggc gccgcaatga
     1741 cggcaaacat catcactgtg ctggcggtgg tggccggcat cgcctggctt ggcgtgctgt
     1801 tcgtctctgc gatccgcaac cgcggcagcg aagagatcgc ccccaatctc aggccaggga
     1861 tcaccgacga ggagatggag acccgtcgtc tcgaaggggg acagaaggcg gccatcgtct
     1921 tctcggcctt cctcgccatc tctcttccct tgtacttcct cggggagccc gcccgccagc
     1981 agggtttcgt cgaggagttc gccgcctcct cgatcacccg cggcgagcac atcgtcaccg
     2041 agtttgcctg cttctcctgc catggccccg agggatccgg cggtgttgcc agctatgtgg
     2101 agaagagatc cggggtgacg gtgagttggg cggcgccctc gctcaatgac atcttcttca
     2161 gattcgacga ggacgaggtc aactattggg tcaccttcgg acgcggtaac accccgatgc
     2221 cgccatgggg ccttcccggc ggtggcccgc tcaacgacga gcaggtggtc gacgtcgtca
     2281 actacctgaa gacgatccag gtctcccagg ccgagaatct cgtcgacgtc gagcccggga
     2341 tcaacaacgc actcgccgcg ctggccgatg ccgatgccac ggtggaggcc gcgatcctga
     2401 agcaagagca ggtggtggct cagatcaact ccgcccccgc agatgtggcg atcgttgttc
     2461 ccctcgccga gagtgctcgt tcggtgctcg acaacgcagt cgacgggctc gacaccgatg
     2521 ccgacggcct gtccgactcg gcagagaccg agctctccgc catctcgcag caggcggtcg
     2581 acgggttcca gatcatggac ccggtcaccc tcgaccccgc cgtccccgat gccgagctcg
     2641 ccgccgaggc tttggccgct ctgcaggccg ccgccgagac cgacccgatc gtcatcacca
     2701 gcatcgatgc gatcgaggag gcattggagc agggtgaggt cgatcccgtt gtcgggctga
     2761 gcccggcgtc gctgaccgca ctcgacgaga cgcgggcggc ggctgagaca gccggcatcg
     2821 aggtcccggc cacggtcgag aacctggccg acgccgaggc actggtggcg gcgctggaag
     2881 aggcggctgc ggccgccgac ccggtggaag ccgcggcagg tctggtgacc gaggcgaccg
     2941 aagctgtcga ggacgggtcg gaccccgacg gcgacgggct ctcctcaggc gctgaggagg
     3001 cggtgagcac ccaggtggcc gcggcgatct cggcaaccac cccgccccag gtgaccctga
     3061 tcactttgga cccgaccaac ccggcatcgg tcggcggcgt gcccgatgcg gcgactgcga
     3121 gccagttcgt cgggaatctc gagtcgctgg tcaccacgct ctcggtcact gccagcaacc
     3181 aggatgcgct tcgcaccacc gaggaagaag ggctggcctt tctccgggag tcgctcgagg
     3241 tcaaggcgta cgagatcgac ttcgatgggg tggccgaagc catgggctca tcggtcgaag
     3301 aggcgcagcg tgcggtgggg ctgttcaact cgacgtgcgc ccgttgtcac accgccggtt
     3361 acagcgccgg ggtcccgttc acccaggagg cggggtccgg tggattcggg ccc
//
LOCUS       DATITW010000091         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_725536, whole genome shotgun sequence.
ACCESSION   DATITW010000091 DATITW010000000
VERSION     DATITW010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725536"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="VLS86_01320"
     CDS             <1..1583
                     /locus_tag="VLS86_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSO49160.1"
                     /translation="LHYATLTDKAAADFETIPGLAESWEASDDGLTYTYTLREGLLWS
                     DDEPLTAEDVAWNINTSRDQGWLNHVSTTQNLEATVIDERTVQITSSVPDPKLPIMDV
                     YLLPKHIWEPVATDAEAVTSYDGTDGVGSGPFTISEFRDQQSVTMVANPNYWGWEGEE
                     PPIDQVVFQLFSNPDAMVAALTQGELDVAHGIPAGSVDSLEANDSIVVVAGQQGGFDE
                     LAINGGAAEGQPHPALLDLEVRRAIGFALDKDAVIEDLWFGLAAPTDAISPSADPKWT
                     PEIPEESRFTYDPDEANRILDEAGYLDTDGDGVREMPDGTNPLVLRHGVNTNTDLGGS
                     IGELFSGWMEQIGIDVELNSYDSDQLYEVIVNGDYDTFYWGWVPYVDPDPMVSYFTTG
                     ELGNNNDANWSDPVYDELYIEQNQELDPERRLEIVHEMLTLFHDSAVYFPLHLSPDLQ
                     AYRTDTFEGWVRQPAEIGPVVFTNSSPTYVQLTPIGGDGGGDSNNLLLWGGIAAGVIV
                     VVGAFLAMRRRSTADERE"
     gene            1642..>2362
                     /locus_tag="VLS86_01325"
     CDS             1642..>2362
                     /locus_tag="VLS86_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49161.1"
                     /translation="MLGSFLTLLFVLVFNFVLFRVVQTDPIGSLFRGRNIPAERLEEL
                     RASFGLDQSMANQFVLYLRETAQLNLGISYQSRQPVWDEIQSRIPATLALVGISAVLS
                     AVIGTIGGVIAAWRRNTWKDRSITSGTMVFYSMPDFWLGMILLVAFAVVLGWFPVGGI
                     VDSGSTATGLARLLDQLHHLFLPALTLTLASLGEYTLVTRSSLLDVMGEDYLTVARAK
                     GLRDVVVRNRHAVPNALLPVVT"
BASE COUNT          432 a          729 c          744 g          457 t
ORIGIN      
        1 acctccacta cgccaccctg accgacaaag ctgcggccga cttcgagacg attcccggtc
       61 tcgccgaatc gtgggaagcg tctgatgacg gactcactta tacctacaca ctgcgtgagg
      121 gcctcttgtg gtcggacgac gaaccgctca ccgcggagga tgtggcctgg aacatcaaca
      181 cctcgcgcga ccagggatgg ctgaatcacg tctcgaccac gcagaacctg gaggcgacgg
      241 tcatcgacga acgaaccgtg cagatcacgt cctcggtgcc cgatcccaag ctgccgatca
      301 tggacgtcta cctacttccg aagcacatat gggaaccggt tgccaccgac gccgaggcag
      361 tcacgagtta cgacgggacc gatggtgtgg ggagtggccc gttcaccatc tccgagtttc
      421 gagatcagca gtcggtgacg atggtggcca atcccaacta ctggggttgg gagggcgagg
      481 agccaccgat cgatcaggtt gtcttccagc tgttctcgaa ccccgatgcg atggtggcag
      541 ccctgaccca gggggagctg gacgtggcgc acggcattcc ggctggctcc gtggactctc
      601 tcgaggcgaa cgacagcatc gtggtcgtcg caggtcaaca gggtggtttc gacgagcttg
      661 cgatcaacgg aggcgcagcc gaaggtcagc ctcatcctgc gttgctcgac ctcgaggtgc
      721 gaagggcgat cggattcgcc ctcgacaaag acgccgtgat cgaggatctc tggttcggcc
      781 tggcggcccc caccgacgcc atcagcccct cagccgatcc caagtggacc cctgagatcc
      841 ccgaggagag cagattcact tacgatccag acgaggcgaa ccggatcctc gacgaggccg
      901 ggtacctgga caccgacggt gacggggtca gggagatgcc cgatgggacc aatcccttgg
      961 ttcttcgtca cggggtcaac accaacaccg atctgggcgg atcgatcggc gagctgttct
     1021 ccggctggat ggagcagatc ggcatcgatg tcgagctcaa ctcctacgac agcgatcagc
     1081 tctacgaggt gatcgtcaat ggtgactacg acaccttcta ttggggctgg gtgccgtatg
     1141 tcgatcccga tccgatggtc tcttacttca ccactggcga gctgggcaac aacaacgatg
     1201 ccaactggtc cgatccggtc tatgacgagt tgtacatcga gcagaaccag gaattggacc
     1261 cggagcggag gttggagatc gtccatgaga tgctcacgct cttccatgac tctgccgtct
     1321 acttcccgct ccacttgagc cctgatctcc aggcctacag gacggacacc ttcgagggtt
     1381 gggtgagaca gccggccgag atcgggcctg tggtgttcac gaactcctcg ccgacttacg
     1441 tccagctcac gccgatcggc ggtgacggcg gcggcgactc gaacaacctg ctgctctggg
     1501 gcgggatcgc ggccggagtg atagtggtgg tcggcgcgtt cctggcgatg cgacgaaggt
     1561 cgacggccga cgagcgggag tagccagctg agcgtcgaac aatcgaagaa acgggggcgc
     1621 ggtcgcctgg tcctgcgcaa ggtgctgggg tcattcctca ccctgctctt cgtcctggtg
     1681 ttcaacttcg tcctgttccg cgtggtacag accgacccca tcggatcgtt gttcagggga
     1741 cgcaacatcc ctgcggagcg gctggaggag ctgcgagcgt cctttgggct ggaccagtcg
     1801 atggcgaacc aattcgtgct ctacctgaga gagacggctc agctgaatct ggggatctcc
     1861 tatcagagcc gacaaccggt ctgggatgag atccagtccc gtatcccggc cactctcgcc
     1921 ctggttggca tctcggccgt tctctcggct gtgatcggca cgatcggagg tgtcatcgcc
     1981 gcctggcgca ggaacacgtg gaaggaccgc tccatcacat cggggaccat ggtcttctat
     2041 tcgatgccgg acttctggtt ggggatgatc ctcctggtgg cgtttgcggt agtgctgggt
     2101 tggttcccgg tcgggggaat cgtcgattcc gggtcgaccg cgaccgggct ggcccgtctc
     2161 ttggaccagc tccatcacct gttcctcccg gcgctgaccc tgaccctcgc ctctctgggc
     2221 gagtacaccc tcgtcaccag aagctcgttg ctcgacgtca tgggcgagga ctatctgacg
     2281 gtggcccggg ccaagggtct tcgcgacgtc gtggttcgca accggcacgc cgtgcccaat
     2341 gccctgctcc cggtggtgac cc
//
LOCUS       DATITW010000092         3221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_4539, whole genome shotgun sequence.
ACCESSION   DATITW010000092 DATITW010000000
VERSION     DATITW010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4539"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3221
                     /locus_tag="VLS86_01330"
     CDS             <1..>3221
                     /locus_tag="VLS86_01330"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HSO49162.1"
                     /translation="EAAAGIRDAAMLMREAAEPLSANSDIAVLSPRELDRELIEFLSS
                     LRSRGVAGSGRLAEILAEAETVADIARTLATREDAGELAVAAEALVATVRGHLHDRQT
                     FEEPSSRDDLSNRLATLAERTAALAEEMDFSFLLNPENKLLSVGYRVGENTLDQSHYD
                     LLASEARLASFVGIAKGDLPPSHWFRLGRSMTPMGVDAALISWSGSMFEYLMPLLVMR
                     SPPESILDTTYRAVVRRQIEYGHERQIPWGISEAAYAARDIHLVYQYRAFGVPGLGFE
                     RGLSEDLVVAPYATLLATMVEPRDAVENLGRLDSLGALGRYGYYEALDFTPTRLPEDQ
                     ERVLVRAYMAHHQAMSLVSLTNVIFSGLCRDLFHADPSVRAAELLLHERVPRDVEVAR
                     PRAEEVAESARPVEEAKGVVRRISSPHGPVPLTHALSNGRLSTVITAAGSGYSQWQGL
                     AVNRWREDATRDNFGSYFFIRDGSGRTWSAGYQPTLAEPDSYQVAFSEDKAEISRRDG
                     SLATRLTVVVSEEDDAEVRRVSITNRGLSAREVEVTSYAELVLAPARADAAHPAFSNL
                     FVQTELVAGRDALLAGRRPRSGEEETVWAGHVLAVTGEVVGGVQYETDRVRFLGRGNQ
                     VHRAAAVGRPLSNTVGRVLDPVFSLRRKVLIQPGATAHLDFTTVVAPSRDEALDLVDK
                     FSDARAFERSLTLAWTQAQVRLHHLRIDPDQAHIFQQLAGTLIYSDPVLRAPRSTEPE
                     ASARQDSLWRHGISGDFPIVLVRIDQPEERGLVRDLLRAQEYWRIKGLAADLVIINEK
                     PGSYAQDLQDAIEELIRESGHLLGQVPDGGVFVLNGPLLSVEDRATLQSSARAVLLSH
                     HGTLADQLARRPAEDLAPTIRPRPVAVDLTGGPQPRPDREFHNGLGGFAADGTEYVVV
                     LGEGQWTPAPWVNVVANPGFGFCVSESGSGYTWAENSRENQLTPWSNDPVSDRPGEVL
                     YVRDEETGEVWGPTALPIRDDLFAYLARHGPGYSEFEHNSRGIALHLHQTVPLEDPVK
                     ISRLRVRNLSGRRRRLSLTAYVEWVLGTTRSGSRTQIVTERDE"
BASE COUNT          517 a         1071 c         1098 g          535 t
ORIGIN      
        1 ccgaggcggc ggccgggatc agggatgcag cgatgctgat gcgggaggcg gccgagcccc
       61 tctccgccaa ctccgacatc gcagtgctgt ctccccgtga gctggatcgg gagctgatcg
      121 agttcctcag ctcgttgcgg agccggggcg tggccgggtc ggggcgtctg gctgagatcc
      181 tcgccgaggc cgaaaccgtg gccgacatag cccgcaccct ggccacgagg gaggacgccg
      241 gggagctggc ggtcgccgcc gaggctctgg tggcgacggt gcggggccac cttcatgacc
      301 ggcagacgtt cgaggagcca tcgagccgtg acgatctctc caatcggctc gcgaccctgg
      361 ccgagaggac cgctgccctc gcagaggaga tggatttctc gttcctcttg aaccccgaga
      421 acaagttgct ctcggtcggc tatcgggtgg gggagaacac tctcgaccag agccattacg
      481 acctgcttgc ctcggaggcc aggctggcca gcttcgtcgg catcgccaag ggcgacctcc
      541 ctcccagcca ctggttccgt ctcggccgct cgatgacccc gatgggcgtc gacgcggccc
      601 tcatctcgtg gtcgggctcg atgttcgagt acctgatgcc gttgctggtg atgcgctccc
      661 caccggagag catcctcgac accacctatc gggcggtggt gcgcaggcag atcgagtacg
      721 ggcacgagcg gcagataccg tgggggatct ccgaggcggc atatgccgcc cgcgacatcc
      781 acctcgtcta ccagtatcgg gcgttcggcg tgcccgggct cggtttcgag cggggtctca
      841 gcgaggacct ggtggtggcg ccatacgcca ctctgctggc gacgatggtc gagcccaggg
      901 acgcggtgga gaacctgggt cggctcgaca gcctgggggc gctgggcagg tatggctact
      961 acgaggcgct cgatttcacc ccgacccgtc ttccggagga ccaggagcgg gtcctggtcc
     1021 gggcctacat ggctcaccac caggcgatgt cactggtttc cctgaccaac gtcatcttca
     1081 gcggactgtg ccgggacctg ttccatgccg acccctcggt cagggcggcc gagctgttgc
     1141 tgcacgagag ggtgccacgc gacgtggagg tggcgcgccc acgcgccgag gaggttgccg
     1201 agtcggcccg tccggtggag gaggccaagg gtgtggtccg tcgcatctcc tcgcctcacg
     1261 ggccggtccc cctcacccac gccctgtcca acggccggtt gagcacggtg atcaccgcag
     1321 ccggctccgg ctacagccag tggcagggat tggcggtcaa tcgctggcgg gaggacgcca
     1381 cccgcgacaa ctttggaagc tacttcttca tcagggacgg atcggggcga acctggtcgg
     1441 ccggctacca accgacactg gccgagcccg actcctatca agttgccttc agcgaggaca
     1501 aggccgagat atcacgccgc gacgggagtc tggccactcg gctcactgtg gtggtgtccg
     1561 aggaggacga cgccgaggtg cggcgggtga gcatcaccaa tcgtggcctt tctgcccgag
     1621 aagtagaggt cacctcctat gccgagctgg tcctggcccc ggcccgggcc gacgccgctc
     1681 accccgcctt ctccaacctg ttcgtgcaaa ccgaactggt ggccgggcgc gacgccctcc
     1741 tcgccggtcg ccggccgcgc tcgggtgaag aggaaacggt atgggcgggg cacgtgctgg
     1801 cagtgaccgg cgaggtggtg ggcggagtcc agtacgagac ggatcgagtt cgtttcctcg
     1861 gccgggggaa tcaggttcac cgggccgcgg cagtcggtcg ccccttgtcg aacacagtgg
     1921 gccgggtcct cgatccggtg ttcagcttgc ggaggaaggt gctcatccaa ccgggcgcca
     1981 ccgcccatct cgatttcacg acggtggtcg ccccgagccg ggacgaggcc ctcgacctgg
     2041 tggacaagtt ctccgacgcc agggccttcg agcggagcct caccctggcc tggacccagg
     2101 cccaggtccg tctccatcat ctgcgcatcg atcccgacca ggcccacata tttcaacagc
     2161 tcgccgggac gttgatctac tccgacccgg tgctccgggc gccgcggtcc accgagcccg
     2221 aagcctcggc gagacaggac agtctgtggc gtcacggcat ctcgggggat ttccccatcg
     2281 tcctggtccg gatcgatcag cccgaagagc gcgggctggt cagggacctg ctccgggccc
     2341 aggagtactg gaggatcaag gggctggccg ccgacctggt gatcatcaac gagaagcccg
     2401 gctcctacgc ccaggatctg caggacgcca tcgaggagct gatccgggag agcgggcacc
     2461 tgctgggtca ggtgcccgac gggggagtgt tcgtgctcaa tggccccctg ctcagcgtcg
     2521 aagacagggc gacgctccag tcgtcggcac gggcggtgct gctcagtcat cacggcaccc
     2581 tggccgacca gctggcgcga agacctgccg aggacctggc gccgacgatt cgacctcggc
     2641 cggtggcagt cgacctgacg ggtgggcctc agccccggcc cgatcgtgag tttcacaacg
     2701 gtctcggcgg tttcgcagcc gacggcaccg agtacgtggt ggttttgggc gaaggccaat
     2761 ggacacctgc gccgtgggtc aacgtcgtcg ccaaccccgg ctttggcttc tgcgtctcgg
     2821 agtcggggtc cggatacacc tgggcggaga acagccggga gaaccagctg actccctggt
     2881 ccaacgatcc ggtatcagat cgtcccggtg aggtgctcta tgtgcgagac gaggagaccg
     2941 gagaggtctg gggacccacc gccctcccga tcagggacga cctattcgca tatctggccc
     3001 gccacggtcc cggctactcc gaattcgagc acaactcccg ggggatagcc ctccacctcc
     3061 atcaaaccgt tcccctcgag gacccggtca agatctctcg tctgcgggtg cgcaacctga
     3121 gtggccggcg tcgccgcctg tcgctcaccg cctacgtgga atgggtgcta gggaccacca
     3181 ggtcgggatc gcgcactcag atcgtgaccg agcgcgatga a
//
LOCUS       DATITW010000093         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_4588, whole genome shotgun sequence.
ACCESSION   DATITW010000093 DATITW010000000
VERSION     DATITW010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4588"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VLS86_01335"
     CDS             complement(<1..397)
                     /locus_tag="VLS86_01335"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSO49163.1"
                     /translation="MAGSVEIEPDDLVQEALVRILRKGSISDLDNPLAYLQKAIVNLA
                     SNQRRSLGRRRKAVARLSVEESWSPSYPADIEAILDLPPKHRAILYLVEVEGVPYAEA
                     ADQWGMTTMAARAMASRAREKARLAMEVLG"
     gene            746..1744
                     /locus_tag="VLS86_01340"
     CDS             746..1744
                     /locus_tag="VLS86_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HSO49164.1"
                     /translation="MSHVSPPPPPSQPAARGRRHRYSRGQRWTIRILLLLVLGTAALV
                     ALFLVTDFQMGDAGQLSALAEGDDVYLIVGSDSRENLPDDLDGSFGDFAGARADVIIL
                     AQVADGRRQLLSIPRDLKVDIPGQGTNKINAAYAFGGPNLLVETVAGATGIRANHYLE
                     VEFGGFAGIVDALGGIELDFPYPARDLKSGLNVDAGTQTVDGATALAYARSRSYEELQ
                     DGEWVAQGGGDIARAARQREVLTEIMAEASSVSGAVRSPWVLGALGSHLTADSDLSML
                     TLARTGWAMRSAGETDSVTLPVHGEDEGGVSYLVRDEPAATEVLDAFAAKVPLPVQ"
     gene            1783..>2150
                     /locus_tag="VLS86_01345"
     CDS             1783..>2150
                     /locus_tag="VLS86_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009851328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HSO49165.1"
                     /translation="MPDPFLDAAIDEAGLGLAEGGIPIGSVLAVDDLIVGRGHNRRVQ
                     KGSPILHGEMDALENAGRLLAVEYRRSVIYTTLSPCHMCTGAILLYGIPRVVIGENRT
                     FLGAEDLLRANRVEVIVVDD"
BASE COUNT          362 a          720 c          698 g          370 t
ORIGIN      
        1 caccgagcac ctccattgcg agccgggcct tttccctggc tctgctcgcc attgctctcg
       61 ccgccatggt ggtcatcccc cattgatccg ccgcctccgc gtagggcaca ccctccacct
      121 cgacgaggta gaggatggcc cggtgctttg gaggaagatc gaggatcgcc tcgatatcgg
      181 ccggatagct gggcgaccag ctctcctcga cggaaagccg agctacagcc ttccgccgac
      241 gaccgaggct gcgccgctgg ttcgaagcca aattcacgat cgccttctga aggtaggcca
      301 gtggattgtc gaggtcggag atcgaccctt tcctcaatat cctcaccagt gcctcctgaa
      361 ccagatcgtc cggctcgatt tcaacggagc ccgccaccgc cgcaatcctt cgcagagccg
      421 ggtagagagc cgttatcagc tccctctccc cgtcctctat ggaagatcgc cttggcaatc
      481 gaaggttctc cctgtcgtca cataagaaga caccgcacac aggcgatatg tgacgaaatt
      541 tctcgacacg gcaagttcat cgaccgagaa cagaacgagg gtaggggcct gacaagcgcc
      601 accgagatcg ccgctgcgta catgggagcg aaggtacgga ggcgggggct cggtttccag
      661 gggtccgcag ccgccgttga gccggagtaa gtcgggggcc agggctaccg tgcccttcgt
      721 cctcctccca atcgagcacc atttcatgag ccacgtgtct cccccgccgc caccgtccca
      781 gccagcagcc cgtggtcgac gccaccgcta cagccgcggt cagcgctgga ccatccgcat
      841 cctgttgctg ctggtgctgg ggaccgcggc tctcgtcgcc ctgttcctcg tgaccgattt
      901 ccagatgggt gatgccggcc agctctcggc tctggctgag ggcgacgacg tctacctcat
      961 cgtcggcagc gacagccgcg agaacctccc ggacgacctg gacgggagct ttggggactt
     1021 cgccggggct cgcgccgacg tgatcatcct ggctcaggtg gcagacgggc gtcgccaact
     1081 gctctcgatc ccccgtgacc tcaaggtcga catcccgggc cagggcacca acaagatcaa
     1141 tgcggcctat gccttcggag ggcccaacct gctggtggaa acggtggccg gggccaccgg
     1201 catccgggcc aatcactacc tcgaggtcga gttcggaggc ttcgccggga tcgtcgatgc
     1261 cctcggaggc atcgagctcg acttccccta cccggctcgc gacctgaagt cggggctgaa
     1321 cgtcgacgcg ggcacccaga ccgtcgacgg cgccacggca ttggcctatg cccgttcccg
     1381 aagctacgag gagctccagg acggggagtg ggtggcgcaa gggggcggcg acatcgccag
     1441 ggctgcccgc caacgcgagg tgctgaccga gatcatggcc gaggcctcct cggtctccgg
     1501 cgcggtgcgc agcccatggg tgctgggcgc cctcggctcg catctgaccg cggactccga
     1561 tctctcgatg ctgacactgg cccgcaccgg gtgggcgatg cgatcagccg gggagaccga
     1621 ctcggtgacg ctcccggtgc atggggaaga cgaaggcggc gtctcctatc tggtgcgcga
     1681 cgagcctgcg gcgaccgagg tgttggatgc cttcgccgcc aaggtccccc taccggttca
     1741 gtaggggtcc gggctcgaga gcaccggacc tagcattcga tcgtgcccga cccgtttctc
     1801 gacgccgcca tcgacgaggc cggattgggt ctggccgaag gcgggatccc catcggctcg
     1861 gtgctggcgg tggacgatct gatcgtgggg cggggacaca atcggcgggt gcagaagggg
     1921 agccccatcc tccacggcga gatggacgcc ctggagaacg cggggcggct gctggcggtg
     1981 gagtaccgga ggtccgtgat ctacaccacc ctctccccat gtcacatgtg caccggcgcc
     2041 atcctgctct acgggatccc cagggtggtg atcggtgaga atcggacctt tctcggcgcc
     2101 gaggacctgc tccgggcgaa tcgggtcgag gtcatcgtgg tcgacgatca
//
LOCUS       DATITW010000094         1514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_685667, whole genome shotgun sequence.
ACCESSION   DATITW010000094 DATITW010000000
VERSION     DATITW010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685667"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VLS86_01350"
     CDS             complement(<1..1078)
                     /locus_tag="VLS86_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HSO49166.1"
                     /translation="MKRVSALVAALVLGLSTVALAQTEAPEIEIVDINGARYAEGGQT
                     LMVVEFRNLPETPDPSRLAISVDGQPVTDLEVAPLGESSVPVGVVLVIDTSGSMEGAP
                     IEAAKSAAKSFVSQKRPEDSIALVTFADTAVVQTGFTTNANDLNSRIDAITATGETAF
                     NDGVILGLTLFDSSAENILPNMIVLTDGEDTVSEATAEQAIAAVEASDARVFGVALES
                     PDFNPDAVAAIAAAGNGLFLSTPDPAELSGLYGQIGREISNTLVARFVSPIAAPGEAE
                     FAVSYPGGLSSTVVFPVSGYATTTTTAPTTTTTAVPVTGEVVQSSSPLALNTLALIGA
                     AGIGITAFLFIVILFGRDEDAGGGR"
     gene            complement(1080..>1514)
                     /locus_tag="VLS86_01355"
     CDS             complement(1080..>1514)
                     /locus_tag="VLS86_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HSO49167.1"
                     /translation="EALDMRQAMNTGHDGSLTTVHSNSPRDTLSRIETMVLMAGFDLP
                     IRAIREQIAAAVDLIIHVSRLRDGTRRVTYVTEVEGMEGDIITLQDIFLFDYGMGVDE
                     DGVYKGRLKATGIRPTFSEDLENQGIRLPADLFDPEPFARGR"
BASE COUNT          260 a          465 c          526 g          263 t
ORIGIN      
        1 aacgcccgcc acccgcgtcc tcgtcccgtc cgaagaggat cacgatgaac aagaaggcgg
       61 tgatgccgat gcctgcagcc ccgatcagcg ccagggtgtt cagtgccagc ggcgatgagc
      121 tctgcacgac ctcgccggtc accgggaccg cggtcgtcgt agtggtggga gctgtggtcg
      181 tggtggtggc gtagcccgag accgggaaga cgacggtcga gctgagacct ccggggtagc
      241 tgaccgcgaa ctcggcttcg ccgggagccg cgatcggcga gacaaaccgg gccacgagcg
      301 tgttgctgat ctcccggccg atctgaccgt acagacccga caactcggcc ggatcgggtg
      361 tcgacaggaa caacccgttg ccggcagccg caatcgctgc cacggcgtcg gggttgaagt
      421 cgggtgactc caaggcgacc ccgaacaccc gggcgtcgct ggcctcgact gccgcgatcg
      481 cctgctcggc ggtggcctcg gacacggtgt cctcgccatc ggtgaggacg atcatgttgg
      541 gcaggatgtt ctccgccgac gaatcgaaga gggtcagacc caggatcaca ccgtcgttga
      601 acgcggtctc accggttgcc gttatggcgt cgattcgact gttgaggtcg ttggcgttgg
      661 tggtgaagcc ggtctggacg acggcggtgt cggcaaatgt gacgagagcg atcgaatcct
      721 cgggacgctt ttgggagacg aagctcttgg cggcgctctt ggccgcctcg atcggggcac
      781 cttccatcga gcccgaggtg tcgatgacca ggacgacacc gaccggaacg ctcgactcgc
      841 cgagaggagc gacctcgaga tcggtgaccg gctggccgtc cacggagatc gcgagccggc
      901 tcggatcggg agtctcgggg agattgcgga actcgaccac catcagtgtc tggccgccct
      961 ccgcatagcg agcgccgttg atgtcgacga tctcgatctc gggggcctcg gtctgcgcca
     1021 gagccactgt gctcagcccg aggacgaggg ccgcgaccag tgcggaaact cgcttcaagt
     1081 cagcgccccc tggcaaacgg ctctgggtcg aagaggtcgg cgggaagcct gatcccctgg
     1141 ttctccagat cctcggagaa ggtgggtcgg atgccggttg ccttgagacg gcccttgtac
     1201 acgccgtcct cgtcgacacc catgccgtag tcgaagagga agatgtcctg gagggtgatg
     1261 atgtcgcctt ccataccttc cacctcggtc acgtaggtga cgcgacgggt tccgtcccgg
     1321 agacgtgaga cgtggatgat caggtcgacc gcagccgcga tctgctcccg gatggcccgg
     1381 atcggcaggt cgaacccggc catgaggacc atggtctcga tacgggacag ggtgtccctc
     1441 gggctgttgg agtggaccgt ggtcagtgag ccgtcatgac cggtgttcat ggcctggcgc
     1501 atgtcgagcg cctc
//
LOCUS       DATITW010000095         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_365035, whole genome shotgun sequence.
ACCESSION   DATITW010000095 DATITW010000000
VERSION     DATITW010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365035"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VLS86_01360"
     CDS             complement(<1..1036)
                     /locus_tag="VLS86_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49168.1"
                     /translation="MKVLAIAGSNVRRMVRERSNIFFVFIFPLALILLIGAQFGGDVD
                     PVIGLYQADDDQLAVAVAEAVEAEDTLEVVDYDTEEQLVSAVERGNAQAGLFLPAGMD
                     QKAEAGELVEIGFVSRTDGFGPQIQSVVGAAVSEVMTHVTAAQFAVVKTGASFEAALE
                     VARSQSAAAPGLGTEVTAIGEALFPSTLGRFDLGASSQLILFVFLTALAGSAALILSR
                     QLGISRRMLATPTATGTIVIGEGTGRWAVAMVQGIYIILATFLIFRVNWGDPLGALLI
                     LITFSAVGAGAGMLMGAVFSNDAQASGIGIVLSLGLAALGGCMLPVELFSPTMQKVAH
                     ITPHAWALDGF"
     gene            complement(1033..>1717)
                     /locus_tag="VLS86_01365"
     CDS             complement(1033..>1717)
                     /locus_tag="VLS86_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49169.1"
                     /translation="PPEVAGWGGEAAARGPAYVLADVTTETRQLDATTYFSAGMAVFF
                     LFFTVQFGVIGLLEEQRDGTMARLMAAPIRRTSVITAKALLSSWLGVISVGVLIVATH
                     YLLGAIWGPPLGVILLVVAAVLSAVAIMGLVGSVAETPEGAGNLGAIIAVVLGMLGGT
                     FFPIGQGDDLLSKLTYLTPHAWFMQGLAEISGGAEWTAALPAVGALLIFALVLGTIGW
                     ALMRRKIHA"
BASE COUNT          346 a          592 c          531 g          248 t
ORIGIN      
        1 cgaagccgtc gagcgcccag gcatgagggg tgatgtgggc caccttctgc atcgtcgggg
       61 agaacagctc gacaggcaac atgcaaccgc cgagcgcggc cagacccagg ctgaggacga
      121 tcccgatccc gcttgcctgg gcatcgttgc tgaagaccgc tcccatcagc atcccggcgc
      181 ctgcgcccac cgccgagaac gtaatcagga tcaggagcgc acccaaagga tcgccccaat
      241 tgacacggaa gatcaggaac gtggcgagga tgatgtagat cccctggacc atggccaccg
      301 cccaccgccc ggtcccctcg ccgatcacga tggtcccggt ggcggtcggg gtggccagca
      361 tccgacgcga tatccccagc tggcggctga ggatcaatgc agccgacccg gccaaggcgg
      421 tgaggaagac gaacaggatg agctgcgacg aagctccgag atcgaaccgt cccagagtgg
      481 atgggaacaa cgcctcgccg atcgcggtca cctcggtgcc caaccccggc gccgctgcgc
      541 tctggctcct tgccacctcg agtgccgcct cgaacgaggc cccggttttc acaaccgcga
      601 actgggctgc cgtcacgtgt gtcatcacct ccgagaccgc ggcgccgact actgactgaa
      661 tctgagggcc gaacccgtca gtgcgggaaa cgaacccgat ctcgaccaac tcgccggctt
      721 ctgccttctg gtccatcccg gccggcagga atagcccggc ttgggcgttg ccccgctcca
      781 ccgccgagac caactgctct tcggtgtcgt aatcgacgac ctcgagggtg tcctccgcct
      841 cgaccgcctc ggccactgca accgccagtt gatcatcgtc ggcctggtag aggccgatca
      901 ccggatcgac atcgcctccg aactgagcgc cgatgagcag tatcaacgcc agggggaaga
      961 tgaacacgaa gaagatgttg gagcgctccc gcaccatgcg acgcacattg gacccggcga
     1021 tggcgagcac cttcatgcgt ggatcttccg acgcatcagg gcccagccga tggtccccag
     1081 gaccagtgcg aagatgagaa gagcacccac ggcgggcaag gcggccgtcc actccgctcc
     1141 acccgagatc tcggccagcc cctgcatgaa ccaggcgtgc ggggtgagat aggtcagctt
     1201 gctcaggaga tcgtcaccct ggccgatcgg gaagaaggtc ccgccgagca tgccgaggac
     1261 gacagcgatg atcgccccca ggttccctgc cccttccggg gtctcggcga cactccccac
     1321 cagccccatg attgccaccg ccgagaggac tgcggcgacg acgaggagaa tcaccccgag
     1381 cggcggcccc cagatcgcac ccagcaggta atgagtggcc acgatgagca cacccaccga
     1441 gatcacaccg agccaggacg agagcagtgc cttggcagtg atgacactgg tgcggcgaat
     1501 cggggccgcc atcaacctgg ccatggtccc gtcacgttgt tcttcgagaa gcccgatgac
     1561 gccgaactgg acggtgaaga acaggaagaa gacggccatc cccgcggaga aataggtggt
     1621 ggcgtccagt tgtcgtgtct ctgtggtgac gtcggcaagc acatacgccg gtccgcgcgc
     1681 cgcggcctcc ccaccccatc ccgcgacctc gggtgga
//
LOCUS       DATITW010000096         1585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_84712, whole genome shotgun sequence.
ACCESSION   DATITW010000096 DATITW010000000
VERSION     DATITW010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84712"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /gene="purF"
                     /locus_tag="VLS86_01370"
     CDS             <1..1125
                     /gene="purF"
                     /locus_tag="VLS86_01370"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HSO49170.1"
                     /translation="PAHRQVGRTSIALGHNGNLTNTRALAERLGVRGATTDSDLMVEG
                     IARALDDERSDARGLEMAIMEVASTWEGAFSLVIMDQGRIVGVRDRNGFRPLCLGSLP
                     EGGWVIASETAALDLVGAQFVREVAAGEMVVIDAGGVHSYSIAPSAKPGHCLFEFDYF
                     ARTDSQMYGRGIHAARRQMGETLAAEHPASADVVVPVPESGLPAAQGFAAASGIPYSD
                     GLVKNRYIGRTFIEPTQSLRDRGISLKLNPIPDNLRDRRVVLVDDSIVRGSTTRNLVQ
                     MVRDAGATEVHLRVSSPPYRWPCFYGMDTSDRSTLIAARLEVDEIREHVGADTLGYLS
                     LQGLLDATKVADAGFCTACLSGEYPTEIPEVTDKFQLERS"
     gene            1127..>1585
                     /locus_tag="VLS86_01375"
     CDS             1127..>1585
                     /locus_tag="VLS86_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HSO49171.1"
                     /translation="MTSYGEAGVDLDGAERHVAGIADAVTATWAGVVGGFGGFAAGVE
                     IPTGYDRPVLMMSTDGVGTKLELARRANRWDGVGFDLVAMCVDDLAVVGAKPLGFVDY
                     LAVGALQLDRDRSIVTSIAAACVEAGCPLLGGETAEHPGIMAPDAVDLAGA"
BASE COUNT          250 a          528 c          545 g          262 t
ORIGIN      
        1 cctgcccacc gccaggtggg gaggaccagc atcgccctcg gtcacaacgg gaatctgacc
       61 aacacccgcg ccctggccga gcgactggga gtgcggggcg cgaccaccga ctccgatctg
      121 atggtggagg gcatagcccg agctctcgac gacgagagat ccgacgcacg aggtctcgag
      181 atggcgatca tggaggtcgc ttcgacctgg gaaggcgcct tctccctggt catcatggat
      241 cagggccgga tcgtcggggt gcgcgaccgc aacgggttcc gaccactctg tctgggatcg
      301 cttcccgaag gcgggtgggt gatcgcctcg gagacagcgg cgctcgacct ggtcggcgcc
      361 cagttcgtcc gagaggttgc cgccggagag atggtggtca tcgacgccgg gggcgtccac
      421 agctactcca tcgcccccag cgccaagccc ggtcactgtc tcttcgagtt tgactacttc
      481 gctcgaaccg acagccagat gtacggccgg ggcatccacg ccgctcgtcg ccaaatgggc
      541 gagaccctcg ccgccgagca tcccgcctcc gccgacgtgg tggtgcctgt ccccgagtcg
      601 ggtcttcccg ccgcccaggg ctttgccgcc gccagcggga tcccgtactc cgacggtctg
      661 gtcaagaacc gatacatcgg gcgcaccttc atcgagccga cccagagcct gagagaccgt
      721 ggcatcagcc tgaagctcaa tcccattccc gacaatctca gggaccggcg cgtggtgctg
      781 gtcgacgatt cgatagtgcg agggtcgacg acgaggaatc tggtgcagat ggtccgcgac
      841 gccggggcca ccgaggtgca tctccgggtg tcctcaccgc cctacaggtg gccgtgcttc
      901 tacggcatgg acacctcgga ccggtcgaca ctgatcgcgg cccgtctcga ggtcgacgag
      961 attcgagagc acgtcggcgc cgacacgctg ggctatctct cgctgcaggg gcttctcgac
     1021 gcgaccaagg tcgccgacgc cggcttttgc acggcgtgcc tctccggtga gtacccgact
     1081 gagatccccg aggtgacgga caagttccag ctcgagcgat cctgagatga ccagctacgg
     1141 agaggccggc gtcgacctgg atggtgccga gcggcacgtg gccggcatcg cagatgcggt
     1201 gaccgcgacc tgggccgggg tcgtcggcgg tttcggcggt tttgccgcag gtgtcgagat
     1261 cccaaccggc tacgaccggc cggtgctgat gatgtccacc gacggggtcg ggaccaagct
     1321 cgagctggcg agacgggcca accggtggga cggcgtcggg ttcgacctgg tggcgatgtg
     1381 tgtcgacgat ctcgccgtcg tgggcgccaa gcctctcggc tttgtcgact atctcgcggt
     1441 gggtgccctc cagttagacc gcgatcgatc gatcgtcacc tcgatcgcgg ccgcctgcgt
     1501 cgaggcgggg tgcccgctgc tgggaggcga gaccgccgag caccccggga tcatggcccc
     1561 cgacgcggtc gacctcgcgg gggcg
//
LOCUS       DATITW010000097         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_765894, whole genome shotgun sequence.
ACCESSION   DATITW010000097 DATITW010000000
VERSION     DATITW010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765894"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..927)
                     /locus_tag="VLS86_01380"
     CDS             complement(25..927)
                     /locus_tag="VLS86_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HSO49172.1"
                     /translation="MTIPDPYWPRADVWLNQEHPDPQVLVVGVPSSKASLSPSRADLS
                     PMAMRDRLARFSTFHGEWGVDFGQVAVSDYGNWPVSELDRDLMPELVTELAGDLPTVP
                     LTLYLGGDNAITRPLVGALAPDLSRIGVLTFDAHHDVRTLELGRTNGNPIRGLIDEDG
                     LPGSNVIQIGIHSFANSAEYRAYCDEVGIGVVTVADIETSGISEVVNASLTTLGDRCD
                     SIYVDVDIDVLDRVFAPACPGARPGGMTVRDLAEGVRRCAQHPKVGAIDFVEVDSEAD
                     QDGMTLDAMAHLMLSAVAGFAERS"
     gene            1119..1436
                     /locus_tag="VLS86_01385"
     CDS             1119..1436
                     /locus_tag="VLS86_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49173.1"
                     /translation="MTEVTRMPRRSYVVEWTMGIAGVLAAGIGAWMYYVPADWFLGGL
                     AEAWYLGFFVGAGVLLAIAFGLTARGMLREDHGFTARATAMTVLAVLAAAGAVTFGLI
                     WIL"
     gene            complement(1501..>2297)
                     /gene="hutU"
                     /locus_tag="VLS86_01390"
     CDS             complement(1501..>2297)
                     /gene="hutU"
                     /locus_tag="VLS86_01390"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HSO49174.1"
                     /translation="PDTYIKAARESMAVHCEAMVGFQTAGAETFDYGNNLRGEAEIAG
                     YADAFSYPGFVPAYIRPLFCEGSGPFRWVALSGDPADIAATDRALGELFQDNDPLHRW
                     LRLASEKVQFQGLPARICWLSYGERHLAGLRFNELVSNGEVSAPIVIGRDHLDSGSVA
                     SPYRETEAMADGSDAVADWPILNAMLNVSSGAAWVAVHHGGGVGIGNSIHAGAQVVAD
                     GTDQGALRVELVLTNDPGTGVMRHADAGYETARQVARERGVDIPML"
BASE COUNT          422 a          732 c          735 g          408 t
ORIGIN      
        1 cgccgatcgg acaaggaacc aggactaaga gcgctcggcg aagccggcaa ccgcggagag
       61 catcagatgg gccatggcat cgagcgtcat tccatcttga tcggcttcgg agtcgacctc
      121 gacgaagtca atcgctccca ccttcgggtg ttgggcgcag cgccgcaccc cttcggccag
      181 gtcgcgcacc gtcatcccac cgggtcgcgc gccgggacaa gcaggggcga agacccggtc
      241 gaggacatcg atgtccacgt cgacgtagat ggagtcacaa cggtcgccca gtgtggtgag
      301 cgaagcattg acgacctctg agatgccgga tgtctcgatg tcggcgacgg tcacgacgcc
      361 gatcccgacc tcatcgcagt aggcgcggta ttcggcggag ttggcaaagc tgtggattcc
      421 gatctggatc acgttggagc cgggcaggcc gtcctcgtcg atgagccctc gaatcgggtt
      481 cccgttggtc ctccccagtt ccagggttct cacgtcgtgg tgggcgtcga aagtgaggac
      541 accgatccgg gagagatcgg gggccagggc cccgaccagg ggccgggtga tggcgttgtc
      601 gcctcccaga tacaaggtca gggggaccgt gggcaggtca ccggccagct cggtgaccaa
      661 ttccggcatc aggtctcggt ccaactctga gaccggccaa ttgccatagt cactgactgc
      721 gacctgaccg aaatccaccc cccattcccc gtgaaaagtc gagaaccggg cgagacgatc
      781 ccgcatcgcc attgggctca gatccgccct cgacggtgag agtgaggcct tcgaggaagg
      841 cactccgacc accaggactt ggggatcagg atgctcctgg ttgagccaga cgtcggcccg
      901 cggccagtaa gggtcaggga tggtcatcgg cttccagggt atcggctgtg ggcaataccc
      961 cgttcatcgg gggggaaagg gccgattggg tacctgcttc gggccccttt gccctagttc
     1021 ggcgaacgcc aatcgtggac actcgaatcg gacgccccga acgccgggtc gtccaaggaa
     1081 gggaggtgat cttcgtgaca acgcttaccc agcgtccaat gacggaggtc acccgaatgc
     1141 cgcgacggtc ttacgtcgtc gaatggacga tggggatcgc cggtgtgctg gcagccggta
     1201 tcggggcgtg gatgtactac gtccccgccg attggttcct tggcggattg gccgaagctt
     1261 ggtacctcgg cttcttcgtc ggagccggtg tgctgctggc cattgcgttc gggctgactg
     1321 cccgaggcat gctgagagaa gaccacggct ttacagctcg cgcgaccgca atgacggtcc
     1381 ttgctgttct ggccgcggcc ggagccgtga cattcggcct gatctggatc ctctgatacg
     1441 catgccagca caggtggggc cccggtcgcc cggggtccca cctttgtgca ttccggccga
     1501 tcacaacatc gggatgtcga ctccacgctc ccgggcaacc tgtcttgcgg tctcgtaacc
     1561 ggcgtcggca tgacgcatga caccggttcc cgggtcgttg gtgagcacca actccacccg
     1621 tagcgccccc tgatccgtgc cgtccgcaac cacctgcgcc ccggcatgga tcgagttacc
     1681 gatccccacg cctcctccgt gatgcaccgc cacccaggcg gcgcccgatg agacgttgag
     1741 catcgcattg aggatcggcc aatcagctac tgcatccgac ccgtcggcca ttgcctcggt
     1801 ctcacggtat ggcgaggcga ccgagcccga gtcgaggtgg tcacgtccga tcacaatcgg
     1861 ggctgacacc tctccgttgg acaccagttc attgaaccgg agcccagcca gatgtcgttc
     1921 gccgtaggag agccaacaaa tccgagccgg aagtccctgg aattgaacct tctcggacgc
     1981 cagccggagc caacggtgca gggggtcgtt gtcctggaac aactcgccga gggctcgatc
     2041 ggtggctgcg atgtctgccg gatcgccgct gagggcgacc cagcggaaag gccccgatcc
     2101 ctcacagaac aacggccgga tgtaggcggg cacaaaaccc ggatacgaga aggcatcggc
     2161 ataccccgcg atctccgcct cgccgcgcag attgttcccg taatcgaagg tctcggcacc
     2221 ggcggtttga aacccgacca tcgcctcaca gtggacggcc atcgactcac gcgctgcctt
     2281 gatgtaggtg tcggggt
//
LOCUS       DATITW010000098         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_605618, whole genome shotgun sequence.
ACCESSION   DATITW010000098 DATITW010000000
VERSION     DATITW010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605618"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VLS86_01395"
     CDS             <1..703
                     /locus_tag="VLS86_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSO49175.1"
                     /translation="GGVAERSDLTLTAADGNRFAAFSAESADSRGPGVVILPDVRGLH
                     QFYAELALRFAENGYDAVAIDYFGRTAGVGRRDEEFPYAEHVPLTTLEGVTADTAAAV
                     AHLRSTDPERPVFTLGFCFGGSNSWHQAAGGHGLAGVIGFYGNPKRPPLGPIEKVSEY
                     RSPLLALMGGDDPGIPIEVVDEFRDALESAGIEHEVVVYPGAPHSFFDRKQKQFAAES
                     ADAWRRALEFIERHS"
     gene            complement(712..2073)
                     /locus_tag="VLS86_01400"
     CDS             complement(712..2073)
                     /locus_tag="VLS86_01400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSO49176.1"
                     /translation="MSRLDDAADVVIVGAGIAGVSVAYHLAVKHEVGGIVVVDPRPPL
                     TLTSDKSTECYRNWWPSKPMVGLMNRSIDLLDQSARDSGNSFGLNRRGYMFATADPTR
                     FRNLVEEANHISMLGAGPVRTHPGPIPYRESPAEGVHGAPEGADVFVGSETVREHFPY
                     LSDHTVGAIHVRRAGWFSAQQLGSWMLDRARERGVDLVVDEVTGIETKGDLVEGVGLK
                     RGGGIRAGTVVNAAGPMSATVAAMAGVGLPLFSELHLKVAYREHLGLVPRDAPLLIWS
                     DTQHLDWTTEERTELAGLGRYDLLGEMPIFCHARPEGGVDSPYLLALWEYHGRVQEPV
                     WPLPEDPLYPEVVMRGLTTMVPGLAEYRDRLPASVVDGGYYTKTSENRPLIGPAGPKG
                     YHLAAGLSGFGVMVAAGAGDLLARHITGADLPDYAPSFLLSRYDDPEYLGAMEMGAGS
                     GQL"
BASE COUNT          360 a          738 c          741 g          342 t
ORIGIN      
        1 cggcggggtc gcagagcgct ccgacctgac cctgaccgcg gcggacggca atcgattcgc
       61 cgcgttcagc gccgagagcg ccgacagcag gggaccgggg gtggtgatcc tccccgatgt
      121 gcgggggctg catcagttct acgcggaact ggcgttgcgc ttcgccgaga acggctacga
      181 cgcggtggcc atcgactact tcggtcgaac cgccggggtc ggcaggcgcg acgaggagtt
      241 tccctacgcc gagcacgtcc cactgaccac gctcgaaggg gtgacggcag ataccgcggc
      301 cgccgtcgcc catctgcgga gcacggatcc ggaacgcccc gtgttcaccc tcgggttttg
      361 cttcgggggc agcaactcgt ggcatcaggc tgccggtggg cacgggctgg ccggggtcat
      421 cggcttctac ggcaacccca agcgacctcc gctggggcct atcgagaagg tgagcgagta
      481 tcggtcaccg ctgctggcgc tcatgggcgg tgacgatccc ggcatcccca tcgaggtggt
      541 cgacgagttc cgcgatgccc tggagtcggc ggggatcgag cacgaggtcg tcgtctatcc
      601 cggcgcccct cactcgttct tcgatcgcaa gcagaagcaa ttcgccgccg agtcggcgga
      661 tgcctggcga cgcgccctcg aattcatcga gcgccacagc tagcggcaag ctcagagctg
      721 gccggagccc gctcccatct ccatcgcgcc caggtattcg gggtcgtcgt atcggctcag
      781 gaggaatgac ggggcatagt cggggaggtc ggcgccggtg atgtggcggg ccaggaggtc
      841 gccggcgccg gcggccacca tcaccccgaa tcccgacaat ccggcggcga ggtggtagcc
      901 cttggggccc gcgggcccga tcagtgggcg gttctccgag gtcttcgtgt agtagccacc
      961 gtcgacgacc gacgcaggaa gacggtcccg gtactcggcc agcccgggga ccatggtggt
     1021 cagcccacgc atcacgacct cgggatagag ggggtcctcg ggaagtggcc ataccggctc
     1081 ctgcaccctt ccgtggtact cccacagagc aagcaggtag ggagaatcga cgccaccctc
     1141 ggggcgggcg tggcagaaga tcggcatctc accgagcagg tcgtatctgc cgagaccggc
     1201 aagctcggtt cgctcctcgg ttgtccagtc cagatgttgg gtgtcactcc agatcaacag
     1261 cggggcgtcg cggggcacca ggccgagatg ctcgcggtag gcgaccttga gatgcagctc
     1321 cgagaacaag gggagcccca ccccggccat agctgccacg gtcgccgaca tcgggcccgc
     1381 ggcgttgacc acggtgccgg cccggatccc gcccccacgc ttcagcccta ccccttcgac
     1441 caggtcaccc ttggtctcga tgccggtcac ctcgtcgacc accaggtcga cgccgcgctc
     1501 gcgggcccga tcgagcatcc aggatccgag ctgttgggca ctgaaccacc cggccctccg
     1561 gacgtgaatg gcgccgacgg tgtgatcgga taaataggga aagtgctctc gaaccgtctc
     1621 cgagcccacg aacacgtccg ccccctcggg agccccgtga accccctcgg ccggggactc
     1681 ccggtacggg atggggccgg ggtgggtccg aaccgggccc gccccgagca tcgagatgtg
     1741 attcgcctcc tccaccaagt tccgaaaccg tgtgggatct gccgtggcga acatatagcc
     1801 gcgtcggttg agaccgaagc tgttgcccga gtctcgagcc gactggtcga gcaggtcgat
     1861 ggatctgttc atgagcccca ccatcggctt gctcggccac cagttccgat agcactcggt
     1921 cgacttgtcg gaggtgagtg tcagcggcgg gcggggatcg acgacgacga tccctcccac
     1981 ctcgtgcttc acggcgaggt ggtaggcgac ggagaccccg gcgatcccgg cgccgacgat
     2041 gacgacgtcc gcagcgtcat ccaaccggct catgtcatct cgatagatgt gcccggaccc
     2101 gggccgccac ctccgggccg gtgaacccga acttctcggc gaggacggtg tcgggcgccg
     2161 aggcgccgaa tcggtcgatc c
//
LOCUS       DATITW010000099         6667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_325361, whole genome shotgun sequence.
ACCESSION   DATITW010000099 DATITW010000000
VERSION     DATITW010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325361"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VLS86_01405"
     CDS             <1..427
                     /locus_tag="VLS86_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49177.1"
                     /translation="VAVSLWGNGAIRILTGFLTLFVAFVVKGAGEAQAAAAGAPALVE
                     NGEDRKGLGALSMFRGSSQSRATEERSPATNKNGNRNAKAGNGKPSPKQGGPPAKGTQ
                     AKQGQGQGQSGRQGTSGNRRSGSRSSGKGKSSGPRRGKR"
     gene            1437..1889
                     /locus_tag="VLS86_01410"
     CDS             1437..1889
                     /locus_tag="VLS86_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HSO49178.1"
                     /translation="MVEADTERAGDFLEGLLDALDVDGDLTTWVDEVGGHIDLEGSDL
                     DVLVGANGETLDALQELTRLAVLRQSKRRVRLLLDINGFRARQREQLITIVKATAEQV
                     IRTREDHEFQPMSPAERKIVHDAVGAIDGAQTESLGEDPHRRVVIRPT"
     gene            1898..2617
                     /gene="rsmG"
                     /locus_tag="VLS86_01415"
     CDS             1898..2617
                     /gene="rsmG"
                     /locus_tag="VLS86_01415"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HSO49179.1"
                     /translation="MKHWSDGDHSPWRPALAARDVELLEQAYAEAIELGFLGPREQER
                     LWQRHLDDAFGLAAIRRPEPGERWADLGSGAGLPGLPLAVAHPTTSFTLIDAQRRRLD
                     WIEATATKLRLTNVSVVHARLEDYGRGPARESFEVATARALGPLPVVAELGLPLLAVG
                     GRLLVPRGQPEPDELEQANLACEQLGARLEEVVPNPSSPIDRVGFVVIMAKIAATSPR
                     FPRRSGMPARTPLGQQRDQKR"
     gene            5418..6443
                     /locus_tag="VLS86_01420"
     CDS             5418..6443
                     /locus_tag="VLS86_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSO49180.1"
                     /translation="MEQEHEDGAIEDEAAIAVREAVTRAAAEAGVDVSRETNQMAPDV
                     SIDELEQEPSVDPEAAARNARVTFRIHHDSGNGTRRVLAVANQKGGVGKSTTAVNIGA
                     YLALAGARVLVVDLDPQGNASTGLGLDHREIEPSIYDVLTGDITPAAAIRATGVANLH
                     VLPSTIDLAGAEVELVSAMSRETRLRRALESIDHQYDTILIDCPPSLGLLTVNALAAA
                     DELLIPIQCEYYALEGLGQLLRNVELVRANLNPELRIGGIVLTMYDGRTRLALQVVDE
                     VRRHFTDVVYQTVVPRSVRLSEAPGYGLPIALYDPLSRGGIAYRDLTLELAERSGLLA
                     PTGEGAS"
BASE COUNT         1219 a         2235 c         2243 g          970 t
ORIGIN      
        1 cgtcgcggtg tcgctgtggg gcaacggcgc gatccggatc ctgaccggct tcctcaccct
       61 gttcgtcgcg ttcgtcgtca agggtgctgg cgaggcccag gccgcggccg ccggggcccc
      121 cgccctggtg gagaacggcg aggaccgcaa gggcctcggc gcgttgagca tgttccgcgg
      181 ctcgtcgcag tcgcgggcga cagaagagcg ttccccggcc accaacaaga acggcaaccg
      241 caacgccaag gccgggaacg gcaagccatc ccccaagcaa ggtggaccac cggccaaggg
      301 aacccaggcg aagcaagggc agggccaggg gcagagtggc cggcagggca ccagcggcaa
      361 ccggagatca ggcagccgct cgtccggaaa gggcaagtcg agtggcccac gccgaggcaa
      421 gcgctaagac ggtcgacgcg gcggtcgccg ccgcggccgc ccagctcggt ctccgttccg
      481 accaggtcga tatcgaggtc ctcgaagacc ccgtcccctc gaccttcggc tacatcggca
      541 cccccgcccg agtccgggtc acctcccgct ccgggtccgc tcccgatacc tcaggcacgt
      601 cgcccaccgg ttcggaggcc gaggcctccg ctcccaaagc agaggcgagt ggtcaggcgg
      661 cctccgaatc agccgttact gccgccgccg accaggccac ctccgatgct ctaggcgcct
      721 tgagttcacc gccgtctgca tcagcgcagg ccgccacctc aactccggcc tcgactccag
      781 gcgacgtccc gattccgggt gacgcctcga ctccgggcga cgacccggcc cgggccacgg
      841 acgcgattga tcccgcgacc tcgcctccgg cctcgcccct cgaccccgcg gccacgaccc
      901 cgccgcctcc gaccctggcc tccgacccct ccccggccct ggactccgat cctgtgccga
      961 gtgccctaac caccgtgacg caaggaacga acgactcgaa cctaacgagt ccctcgtata
     1021 cctcagactc ctcctctacc ccgaaggtga ctggagttgg caccggatcg ggcgcgggtt
     1081 cagagggagg acaggcttcg gcgggcttcg ggggtctggg ctcgggaacg ggatctgctg
     1141 gtgcggattc ggttgtgggc ccggacgtgg gtacggtcgt gaaggcagga agtgacgaga
     1201 ccaccccgtc cacaggcgcg ccagaaaccg ccactgtcgc tgcgtccccc acggcgcagc
     1261 cttcctcggc gaccgcgccg gggggcaatg gcgatcgagc ctcagaggta gaggcgacca
     1321 ggggatcctc gccgctctcc acaccgccag ccaccccgcc gcctcccggc tcggaaccgc
     1381 cgagcacgac caggcctcca cgtcgcacgt accgcgacga accaatcgag ccggagatgg
     1441 tcgaggctga taccgagcgg gccggcgact tcctcgaggg acttctggac gccctggacg
     1501 tcgacggcga cctcaccacc tgggtggatg aggtgggcgg ccacatcgac ctcgagggct
     1561 ccgacctgga cgtgctcgtg ggagccaatg gggagaccct cgacgccctc caggagctga
     1621 cccgtctcgc cgtcctccgt cagtcaaagc gacgggttcg gttgctcctc gacatcaacg
     1681 gcttccgtgc tcggcaacgc gaacaactga tcaccatcgt gaaggcgact gccgaacagg
     1741 tgatccgtac ccgcgaggat cacgagttcc agcccatgag cccggctgag cggaagatcg
     1801 tccacgacgc cgtaggcgcg atcgatggcg cccaaaccga gagcctgggc gaagaccctc
     1861 accggcgcgt ggtcatccgg cccacctagc gtttcacgtg aaacattgga gcgacggcga
     1921 ccattcgcct tggcgtccgg cgctcgccgc ccgcgacgtg gagctcttgg agcaggccta
     1981 tgcggaggct atcgaactcg gcttcctagg accgcgggag caggaacggc tctggcaacg
     2041 ccatctcgat gatgccttcg gcctggcggc gatccgacga cccgagccgg gggagcgctg
     2101 ggccgacctc ggatcaggcg ctggattgcc cgggctccct ctcgccgtag cgcatccaac
     2161 cacctcgttc accttgatcg atgcgcaacg tcgtcgactg gactggatcg aggcaaccgc
     2221 caccaagttg cgcctcacca acgtctcggt cgtccatgcg agactcgagg actacggccg
     2281 cggccctgcc cgggagtcct tcgaggtggc aacagcaaga gcgctcgggc cgcttcccgt
     2341 ggtcgctgag ctcggccttc cgttgctggc cgtcggagga cgactgctcg tcccccgagg
     2401 gcaaccggag cccgatgagc tcgagcaagc caatctcgcc tgcgagcagc tcggcgcgcg
     2461 cctcgaagag gttgtcccca acccgagttc gccgattgac cgggtcggtt tcgtcgttat
     2521 catggcaaag atcgctgcga cctccccccg cttcccccgg cgatcgggca tgccggcgcg
     2581 aactccgctc ggccagcagc gcgatcagaa gcgctagctg acaccaagcg tcgatcggag
     2641 tgccgtcagg atggtggtgt aggttgctgc gggatcgagg agacaagcag ggacggtatc
     2701 tggtcgtgga ccggcaccgt cttggctttc ggcattttgt ggtcggagtg gcggtaggcc
     2761 gaacgtacgg cccggggtcg ggttggcggc atcaggagcc agttcgagcg tcgggcgtgc
     2821 tcgaccagcg ctcccgggtc ccacgcgccg cctcgtagtt gttgtgaagg gaggcgggag
     2881 ccatcagtcg gcgacccgat gaggcaccgc aggtccaaga agaaccaacg ggcgggttcc
     2941 tgagcaggct gctagccgca ctggggctac gccggcgacg gagaaccgag gaccacgcag
     3001 gagcaggcgt acgcgagcag ggtaaatcgg tgccgtcgga atcgccgtcg acgggcaccg
     3061 ccgggggggc cggcatcgcg gaagtgtcac ccgcaggcga gaggagcggg cgcggtgccg
     3121 tcataggatc agcacaggac ggtgaaacgc cgacggtgaa tcagtctcgg gagtggccgt
     3181 ttcgcgatac cgcacccagg caggacccag ccgaagctcg acacgaccca atctggggaa
     3241 ccgacccacc ggacgccgtc cggtggcaca tggacatgac tcccgaccag gagcccatca
     3301 cctccaggtc gccaacgacg aatggggatg tggcaggcca ggagtcggtc gacgccgacg
     3361 acgtacagca ggttgaacag ccgttggcag aacaggtatc ggtcgagcag gtccaggtgg
     3421 atcagggtga ccatgccgca ggtcctgatc gggaagccgg ggtggccggt ccggacggcc
     3481 aggatctcga gcaggaggta cccgcgcagg cctacgaccg atctgagggt ctcgtcgttg
     3541 agcagcccac ttcagagggc gagaacgctg accccgaact gccggatccc cctggccaac
     3601 acgttagatc agcatcagct gacggcgcga cggtgcgggg cggtcctccg ggtggcgctg
     3661 ggtcgagcgg tggcggcgac atttctccgc gagcagcagt ggctacggcg gctgaggaat
     3721 cggcaatcgg gcccgccgga tccctgcacc gcgacgagtc cggcgtcggt tggttttcga
     3781 cagagcccgg caccagccag cacagcgaga tgctctcaga agaactgagc gccgaacgcc
     3841 cgagcgacga gccagctcgg tcctctggac ctgagggttt cccaagcggg gggacaccgc
     3901 atgttgggat cgggctcacg ccgggtcgag gggagcatcg aggtccagag ctgatcgaga
     3961 acgggatggt ccacgtcgac cccgatcctg agatcggatc tccgcctcgc gcggccggat
     4021 ggaccgaaca ccaggccagc cagaccgaat cggaggagcc tggtcgttcg ccagtggagg
     4081 gtcggcctgc ggcggtcgct ccagggcctt cccaggtacg cgacagttcc agctccacgg
     4141 atctcgttgc ccgcgcgacg gctggaccag gtccaggcac ctcagatgtc gagacggaag
     4201 cccgactcgc gcgtgatggc tcagaatccg tcgtcggtga cggaagcgat gccgacgcgg
     4261 ttgttcggga tcaggggatc gctaccgctc ccgatccgag cacaaacgcc gaggatgatg
     4321 aagggaccga cgccacctcc gcagggaccc agaccgcagg gaccgaggcc tccgcggcaa
     4381 ccaagggctc caccgaggcc gggggccccg cgacagggac caaggcctcc ggtcctccat
     4441 cgaacgccat cggcgaaccg ggagcccgtg ttccgcgacc ggaggtagag gcctcgccgg
     4501 caaaccctct ggctccgctc tcgtcctctc ctcagtccgg cgaggctgcg accgtgggcg
     4561 caaggacaaa cgacgggtcc atcggcagcg gctcattcga agtccctccg gccagcccag
     4621 acgctgctcc ggaaggctcc aggctcgtag atgcgatcgg ttccagtagt agggccgaaa
     4681 ccggcggcga gcaggtcccc tggaacgaac cagcgctacc gatggcatgg ccgccggtga
     4741 tggagccatc agaccgtgcc gagcgacgtg agccgaccga cgaggggtca cccgacgacg
     4801 agctccgacc ccagccagat ccaacttggc cgcgcctctt cagtcggacc cctgagggtc
     4861 aggacatcgg tgggacggcg gagtcgaatg acccgggacc cgtcatcggg cctgctggcg
     4921 agggcacggt gccggagtcc ccacatgagt cgagccagcg tgttgcttcg ccggcctccg
     4981 catgggctgc cgggggtaaa ccggccacag atcacgcggc gccgtccaat ggggaggaag
     5041 aggcatcctc agggcaggag catcggcagt cgggtgtcgc tccatcgaga tctaatgacc
     5101 cgagcaacgc cccatcggag gtcgaggacg acctgaccaa ttcggcgacg gtcgtcgacc
     5161 tcacaaccgg agacgcagag cgcgttcgtg gatccctgaa ggttcctgag gttgatgggc
     5221 aagctgcgag cacaggccgc caggactccg ccgcggagcc cggaacggcc gtcggggctg
     5281 gcagcgccac aacgagcccc gcgaacacct cgaccactac gcgagtcgaa gcagaggaag
     5341 tagacctgcg agatgcatcg gcggatccag cgaaggtcga tgtttcacgt gaaacatctg
     5401 taggcggtgg taaagggatg gaacaagaac acgaggacgg ggccatcgag gatgaggctg
     5461 cgattgcagt tcgtgaggct gtgacccggg cggcggcgga agctggcgtc gatgtttcac
     5521 gtgaaacaaa ccagatggcg ccagacgttt cgatcgacga gctggaacaa gagccgagcg
     5581 tcgaccccga ggcggcagcg agaaatgccc gagtcacctt ccgtattcac cacgactccg
     5641 ggaacggcac ccgccgcgtc ctcgccgttg ccaaccagaa gggtggagtg gggaagtcca
     5701 cgacggccgt gaacatcggg gcgtaccttg cgctcgcagg agcacgagtc ctggtggttg
     5761 atctcgatcc gcaaggcaac gcatcgacgg gcctcggtct cgatcatcgt gagatcgaac
     5821 catcgatcta cgacgtgctc accggcgaca taaccccggc ggcggcgatc cgcgcgactg
     5881 gggtcgcgaa cctccacgtc ctgcccagca cgatcgacct cgccggtgcg gaggtcgagt
     5941 tggtgagtgc gatgtcgaga gagactcgcc tccggcgagc tcttgaatcc atcgaccacc
     6001 agtacgacac gatcctgatc gactgcccgc cgtcgcttgg gctgctcacg gtgaacgcct
     6061 tggccgcagc ggatgagctc ctgatcccga tccaatgcga gtactacgcg ctcgaggggc
     6121 tgggccaact gctccgcaac gtcgagctcg tccgggcgaa tctcaacccg gagcttcgta
     6181 tcggcggcat cgtgctgacg atgtacgacg gccggacccg gttggcgctg caggttgtcg
     6241 acgaggtccg cagacacttc acggatgtgg tgtatcaaac ggtcgtgcct cgaagtgtcc
     6301 ggctgtcgga agccccgggc tatgggcttc cgatcgctct ctacgatcct ctttcgcgtg
     6361 gcggcatcgc ctatcgcgac ctgacccttg aactcgcgga gcgaagtggc ttgctcgcgc
     6421 cgaccggaga aggagccagc tgatgcgacg ccgtggtggc ctcggccgtg gactggaagc
     6481 gctgattcca agcagcactc aggacgatcg cgaaccggcg actcagccca ccgaccagaa
     6541 gcctgtggcg gcagaggagg ttacggactc cgctggaccg acgttcgagc agcttctgat
     6601 cgaccggatc gatcccaacc cgcgtcagcc tcgtgaggcc ttcgacgagg agccgcttca
     6661 ggaactc
//
LOCUS       DATITW010000100         1591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_4855, whole genome shotgun sequence.
ACCESSION   DATITW010000100 DATITW010000000
VERSION     DATITW010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4855"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VLS86_01425"
     CDS             complement(<1..1010)
                     /locus_tag="VLS86_01425"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HSO49181.1"
                     /translation="MFTRVPSDYKSDRRRPLTLILIGVAVVYLFLTALGTLWTDYLWF
                     DSIGFEGVWQRRWGLSIVLGAIGIAVAFAILWVSLQLVDRMSPRWAPFDLTEEEELIE
                     RFREWVEPRIRQVRFWLTGGLAVLLGLTVSTWRDEVFLFLNSQEFGVVDPIFEQDIGF
                     YMFRLPLMGTVVDWVFNLLVLTTVVVAVAHYFNGGLRFNGRRFTTTRPTKTHISVMFA
                     LIAVVRAVVYRLDMYELLFSDRVADKFFGPGYADITARLPAYRLLILVALLAAVLFIV
                     NIFRPGWSLALVAVGSWLVVAVAAAAIYPAFIQRFQVTPDPFDKEAPYIEHSLEFTKA
                     AWG"
     gene            complement(1076..>1591)
                     /locus_tag="VLS86_01430"
     CDS             complement(1076..>1591)
                     /locus_tag="VLS86_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49182.1"
                     /translation="VTDAQNVALDSLRTEDGWYPDGPLLAEMMRADLIGLWAESYSAG
                     HTTAEEMAGAKLRRRDTPASDAAEVFGEDLAAAVSDALIDAGDGQREKQAATSRVFRG
                     WRTDEAERRVRELALIGYHRGLVESVSGQGDLEWVPSGTPCSSCRAASTDPANNTPPV
                     HAGCACTLALV"
BASE COUNT          319 a          531 c          504 g          237 t
ORIGIN      
        1 ccccacgccg ccttggtgaa ctcgagactg tgctcgatgt acggggcctc cttgtcgaaa
       61 ggatcgggag tcacctggaa acgctggatg aacgccgggt agatggccgc cgcggccacc
      121 gccaccacca gccacgatcc aacggcgaca agggcgagac tccaacccgg tctgaagatg
      181 ttgacgatga acaacaccgc ggccagcagc gccaccagga tcagcagccg gtaggcggga
      241 agacgcgccg tgatgtcggc atatccggga ccgaagaact tgtccgcgac gcgatcggag
      301 aacaacagct cgtacatgtc gagccggtag accaccgccc gcaccacggc gatcaaggcg
      361 aacatcaccg agatgtgggt cttggtgggt cgggtggtcg tgaaccggcg cccgttgaag
      421 cggagccctc cgttgaagta gtgggccacc gcgaccacaa ccgtggtcag gaccagcagg
      481 ttgaacaccc agtcgacgac cgtgcccatg agcggaagcc ggaacatgta gaagccgatg
      541 tcctgttcga atatcgggtc gacgacgccg aactcctgtg agttgaggaa cagaaatacc
      601 tcgtcacgcc aggtggacac ggtgagcccg aggagcaccg ccagaccacc ggtcagccag
      661 aatcgaacct gacggatccg gggctccacc cactcacgga agcgctcgat cagctcctct
      721 tcctcggtga ggtcgaaggg cgcccaacgc ggtgacatgc gatcgaccag ctgcaagctc
      781 acccagagga tggcgaaggc aactgcgatt ccgatcgccc cgaggacgat ggagagaccc
      841 catctccgct gccacacgcc ttcgaacccg atcgagtcga accagagata gtcggtccac
      901 agcgtgccga gagccgtcag gaacaggtag accacggcca ccccgatcag gatcaaggtc
      961 agcggccgtc ggcggtcaga cttgtagtcc gagggaaccc gggtgaacat cggccctgag
     1021 ggtaacggct ctgcggatca gcgttccgcg tgggtcgaca cgccggtgat cgagctcaga
     1081 ccagggccag ggtgcatgcg cacccggcgt gcaccggagg agtgttattc gccgggtcgg
     1141 tggatgccgc ccgacatgag ctgcatggcg tgccgctcgg cacccattcg agatcaccct
     1201 gccccgacac cgactccacc aggccgcggt gatagccgat gagggcgagc tctcgcacac
     1261 gacgttccgc ctcgtcggtt cgccaacccc tgaatacccg cgaggttgcc gcctgtttct
     1321 cacgctgacc gtcaccggcg tcgatcagcg catcggagac tgcggccgcc agatcctccc
     1381 cgaagacctc ggcggcgtcc gatgccggtg tgtcccgacg tctcagcttc gcgcccgcca
     1441 tctcctcggc ggtggtgtgg ccggccgagt agctctccgc ccagagcccg atcagatccg
     1501 cccgcatcat ctcggcaagc agcggcccgt cggggtacca gccatcctcg gtgcgcagac
     1561 tgtcgagggc cacgttctgg gcgtcggtga c
//
LOCUS       DATITW010000101         1608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_205263, whole genome shotgun sequence.
ACCESSION   DATITW010000101 DATITW010000000
VERSION     DATITW010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205263"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VLS86_01435"
     CDS             <1..264
                     /locus_tag="VLS86_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HSO49183.1"
                     /translation="RRLERVAVVPGSGAGFIEEAATLADAIVTGDVSHHRAVHAMDLG
                     LAVVDPGHIATERPGMVALVALVADVVGGRVVDLTELDPETWG"
     gene            289..996
                     /locus_tag="VLS86_01440"
     CDS             289..996
                     /locus_tag="VLS86_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HSO49184.1"
                     /translation="MEITSLADLLDVQDLDSQIDRLLEKRQTLPELSNYKAAHEQEQA
                     LNEELEAAGSELKVLELDFDKSEGELQILEAKLQEHETRLYAGGMSGRETEHMRLEVQ
                     SLKGQREALEERVLNMLEQLDPAREQVADLRSRLDAVAVEKSGFETTIKAEWKVIDAE
                     LARKEERKKEALSPIPPDLVEMYEKLRHSKEGVAIGRYEHGVCGGCHMALSPAEEVEA
                     LGAEIPRCVHCRRILVA"
     gene            1000..1539
                     /locus_tag="VLS86_01445"
     CDS             1000..1539
                     /locus_tag="VLS86_01445"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HSO49185.1"
                     /translation="MVFWHLGATLWLFRWIFRDPKVDVRFLLAGALLPDVIDLLLGTV
                     IFAERYSTGELWSHTLLVPSLYMTVVLLLTRRGRRRRAYMALGIGWLLHLLLDGMWTD
                     PELFFWPFFGWEMPQGAAPFWPLAWERAMSDPWRWILEAVGLAYLVWLWFAVGLNHRE
                     ARQSVRRTGRLPEYVSEGA"
BASE COUNT          264 a          509 c          559 g          276 t
ORIGIN      
        1 cgacgcttgg agagggtcgc cgttgtacct ggatccgggg ccggattcat cgaggaggct
       61 gccactctgg ccgacgccat cgtgaccggc gacgtgtccc accaccgcgc cgtgcatgcc
      121 atggacctcg ggctcgccgt cgtcgacccg ggtcacatcg ccaccgagag accggggatg
      181 gtggctctcg tcgctcttgt cgccgatgtg gtcggcggac gggtggtcga tctcaccgag
      241 ctcgaccccg aaacatgggg ctgactcacc gctaccctcc cgtcctgcat ggagataacg
      301 agcctcgccg atcttctcga cgtccaggat ctcgattctc agatcgaccg cctcctcgaa
      361 aagcgccaga cgctccccga gctgtccaac tacaaggccg ctcacgagca agaacaggcc
      421 ctcaatgagg agctggaggc ggcgggatcg gagctgaagg tcctcgagct cgatttcgac
      481 aagtcggaag gcgagctgca gatactggag gccaagctcc aggagcacga gacccgtctc
      541 tacgcgggtg ggatgagcgg gcgggagacc gagcacatgc ggctcgaggt gcagagcctc
      601 aaggggcaac gggaggccct ggaagagcgg gtgctcaaca tgctggaaca gctcgacccg
      661 gcccgggagc aggtggccga tctcaggtcc cgtctcgatg cggtggcggt cgagaagtcc
      721 ggattcgaga cgacgatcaa ggcggagtgg aaggtgatcg acgccgagct ggcccgcaag
      781 gaggagcgga agaaggaggc gctatccccg ataccgcccg acctggtcga gatgtacgag
      841 aagctccgtc acagcaagga aggcgtcgcc atcggccgct acgagcacgg ggtgtgcggc
      901 gggtgccaca tggccctgtc accggccgag gaagtcgagg cgctgggcgc cgagatccca
      961 cgatgtgtcc actgccggcg gatcctcgtc gcctgagaca tggtgttctg gcacctcggg
     1021 gccaccctgt ggttgtttcg ctggatcttc cgcgacccga aggtggacgt caggttcctc
     1081 ctcgccgggg cgctgctccc cgatgtcatc gatctcctcc tggggaccgt gatcttcgcc
     1141 gagcggtact cgaccggaga gctgtggtcg cacacgctgc tggtcccctc gctgtacatg
     1201 accgtcgtgt tgctcctcac ccgaagggga cgtcgtcgtc gcgcctacat ggccctgggg
     1261 atcggctggc tgctgcatct gctgctggac gggatgtgga ccgaccccga gctcttcttc
     1321 tggcccttct tcggctggga gatgccccag ggtgcagccc cgttctggcc attggcctgg
     1381 gagcgagcga tgtcggaccc atggcggtgg atcctcgagg cggtggggct ggcctacctg
     1441 gtctggctct ggttcgcggt gggtctcaac caccgggagg cccgtcagag cgtgagacgc
     1501 accggccggc tgccggagta tgtgagtgag ggcgcgtaac gccctcgctg tcgtgctgct
     1561 ggtggccgcc gcatgcagcg gcgacaaccc ggcgtcgtcc acggtgac
//
LOCUS       DATITW010000102         1980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_45121, whole genome shotgun sequence.
ACCESSION   DATITW010000102 DATITW010000000
VERSION     DATITW010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45121"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VLS86_01450"
     CDS             complement(<1..523)
                     /locus_tag="VLS86_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HSO49186.1"
                     /translation="MIHLLALPGAPRFNGSIDEIVNAAVSDAEALRGAGFPALLVENF
                     GDAPFHADRVEPETIAAMTVAVEAVIAATSLPVGVNVLRNDAIGALGVAAVTGARFIR
                     VNVLTDLMYTDQGPIVGRAAELLRRRRVLAPDVEIWADVMVKHATPPPGLEPAHAALD
                     TVERGLADAVIVSG"
     gene            complement(553..1284)
                     /locus_tag="VLS86_01455"
     CDS             complement(553..1284)
                     /locus_tag="VLS86_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HSO49187.1"
                     /translation="MRDILSDLVAEQQFLDQSLQRIPIKIWDMVTPSKPWTVRDTISH
                     LADFEELAADTIAGGNKIQGWRNASDLDGLRKEAIKKGRAMRPQDVIEWWRGGRAKVV
                     EPLSHMDAMSRIEWIEGDMSARTFATFRLMETWAHGLDIYATLGLEVEDTSRIRHVCW
                     LGWKTLPHAFKEAGEDYEPVRVEVIGPGYAKWVYGPADTNQVIKGSASDWARVAVRRA
                     KPESTRLKYEGEHARKALQVAQAYL"
     gene            complement(1335..>1980)
                     /locus_tag="VLS86_01460"
     CDS             complement(1335..>1980)
                     /locus_tag="VLS86_01460"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HSO49188.1"
                     /translation="EARTVNFKYFFTRKLAFVKESHAFAILPGGWGTLDEGFELLTLI
                     QTGKSDMHPIVLLEAPGTNYWDPMIDFIKEHQLQRGLISPTDLALFLHTTDPVEACDH
                     IWGFYANYHSQRYAGGRLILRLVQGPNDAQLEELNREFADIVVEGEIERAEPAPSEIS
                     DGDALDTDRISFVFDRRQFGRLHQLIDHLNGLVATPESFELPEPMTEEQAERPW"
BASE COUNT          343 a          602 c          661 g          374 t
ORIGIN      
        1 ggccggagac gatgaccgcg tcggcgagtc cccgttccac ggtgtcgagg gcggcgtgag
       61 cgggctcgag accgggagga ggggtggcgt gcttgaccat gacatcggcc cagatctcga
      121 catcgggagc caggacacgg cggcggcgaa gtagctcggc agcgcgaccc acgatcggcc
      181 cctggtcggt gtacatcagg tcggtgagca cattgaccct gatgaagcgg gctccggtca
      241 ctgccgccac tcccagggcg ccgatcgcgt cgttgcgaag gacgttgaca ccgacgggga
      301 ggctggttgc cgcgatcacg gcctcgaccg cgaccgtcat cgccgcgatc gtctccggtt
      361 ccacccggtc ggcatggaat ggggcgtcac cgaagttctc caccagcaat gcgggaaagc
      421 cggcgccgcg cagggcctcg gcatcggaaa ccgcggcgtt cacgatctcg tcgatcgatc
      481 cgttgaaacg cggcgccccc gggagggcaa gcagatggat catccccacc aggtggggaa
      541 gcccactcac actcacagat aggcctgggc tacctggagg gcttttctgg cgtgttcgcc
      601 ttcgtacttg agacgggtgg attcgggttt tgctctacgg accgcgaccc gagcccaatc
      661 cgacgcggag cccttgatca cctggttggt gtcggcaggc ccgtagaccc acttggcata
      721 tccggggccg atcacctcga cccgaaccgg ctcgtagtcc tcaccggcct ccttgaaggc
      781 atgggggagt gtcttccacc ccagccaaca gacgtgccgg atccgggagg tgtcttcgac
      841 ctcgaggccg agtgtggcgt agatgtcgag gccgtgggcc caggtctcca tgagacggaa
      901 cgtggcaaag gtccgtgcgc tcatgtcccc ctcgatccac tcgattctcg acatggcgtc
      961 catgtgggag aggggctcga ccaccttggc tcggccgccc cgccaccact cgatgacgtc
     1021 ttgaggcctc atggcgcggc ctttcttgat cgcctccttg cggagcccgt cgaggtcgga
     1081 ggcattgcgc cagccctgga tcttgttgcc accggcgatg gtgtcggcgg ccaattcctc
     1141 gaagtcggcg aggtgggaga tggtgtcgcg cacggtccac ggcttggagg gcgtgaccat
     1201 gtcccagatc ttgatcggga ttctctgcag ggactgatcc aggaactgct gctcggctac
     1261 caggtcggaa aggatgtcgc gcacgttcca atggtagtag ccatctgcaa gctattccca
     1321 ggcgaaacta cctctcacca gggtctttcc gcctgctcct cggtcatcgg ctcgggcaat
     1381 tcgaagcttt ccggtgtcgc caccagcccg ttgaggtggt cgatcagctg gtggagacgg
     1441 ccgaattggc ggcgatcgaa tacaaatgag atgcgatcgg tgtccagcgc gtcgccgtcg
     1501 ctgatctcgg atggcgccgg ttcggctcgc tcgatctcgc cttcgaccac gatatccgcg
     1561 aactcccggt tcaattcctc gagttgggcg tcgttgggcc cctggacgag gcgaaggatc
     1621 agccggccgc ccgcgtaacg ctgggagtgg tagttggcgt aaaagcccca gatgtgatca
     1681 caggcctcga cgggatcggt ggtatgcagg aagagggcga ggtcggtggg ggagatcaac
     1741 ccacgctgca attggtgctc cttgatgaaa tcgatcatcg ggtcccagta gttggtcccg
     1801 ggagcctcga gcaggacgat cgggtgcatg tcactcttgc cggtctggat gagggtgagc
     1861 agctcgaatc cctcgtccaa ggtgccccat ccgccgggga ggatggcgaa ggcatgcgat
     1921 tccttgacga aggccagttt tcgggtgaag aagtacttga aattgacagt gcgcgcctcc
//
LOCUS       DATITW010000103         1636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_45163, whole genome shotgun sequence.
ACCESSION   DATITW010000103 DATITW010000000
VERSION     DATITW010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45163"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VLS86_01465"
     CDS             complement(<1..1276)
                     /locus_tag="VLS86_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSO49189.1"
                     /translation="MTATRDSYEAKDITVLDGLEAVKRRPGMYIGTTGPRGLHHLIWE
                     VVDNSVDEAMAGFCTKIMVTLRADGGVEVIDDGRGIPVERVEKSKKSALTTVLTTLHA
                     GGKFEQGAYTVSGGLHGVGVSVVNALSSRLEAEVRRGGFAYTQEFRDGQPKAKEPKKG
                     RPIKKSGTTIRFWPSPETFQETTSFEYDTVASRLRETAFLNRGLHITLTDEREAEPRT
                     ESFQYKGGLVDFVKHLNSKRDPLHAHVIDISDVGDDAELDVALQWTTSYTESVLSFAN
                     NINTHEGGTHEEGFRKALTKAINDFARAKGLLKEKDENLTGEDVREGLTAVLSVRVKE
                     PQFEGQTKTKLGNTEIRSYVERALNRKLPEWLERYSPEARRIVEKCVSAARAREAARK
                     ARELTRRKSGLESGGLPDKLADCSSRDPGESEL"
     gene            complement(1395..>1636)
                     /locus_tag="VLS86_01470"
     CDS             complement(1395..>1636)
                     /locus_tag="VLS86_01470"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HSO49190.1"
                     /translation="PDPVLMSQLVDEWDELAGNPWVGRSRPVVIQGRTLVVEASSPSM
                     VAFLRYGSAQLLESLKARFGEGVVDQIEVTPPTRN"
BASE COUNT          264 a          541 c          507 g          324 t
ORIGIN      
        1 acaactccga ctcgcccggg tcgcgactcg agcaatcggc gagtttgtcg ggtagcccgc
       61 cggactcgag acccgacttg cgacgggtga gctctcttgc cttgcgcgcc gcctcccttg
      121 ccctcgccgc gctgacacat ttctcgacga tgcgccgggc ttcgggcgaa tagcgctcga
      181 gccactcggg gagcttgcga ttgagcgccc tctcgacata ggagcggatc tcggtgttgc
      241 ccagcttggt cttggtctga ccttcgaact gcggttcctt gacccgcacc gagagcacgg
      301 ccgtgaggcc ctcgcggaca tcctccccgg tcagattctc gtccttctcc ttcaacaagc
      361 ccttggcccg ggcgaagtcg ttgatcgcct tggtcagcgc cttgcggaac ccctcctcat
      421 gggtgccgcc ctcgtgggtg ttgatgttgt tggcgaacga cagcaccgac tcggtgtagg
      481 aggtggtcca ctgcagggcc acgtccaact cggcgtcgtc gccgacatcg gagatatcga
      541 tcacgtgggc gtggagcggg tcccgcttcg agttgaggtg cttcacgaag tcgacgagac
      601 cgcccttgta ctgaaagctc tcggtacggg gctcggcctc ccgctcgtcg gtgagggtga
      661 tgtgaaggcc gcggttgagg aaggcggtct cgcggagacg ggacgccacg gtgtcgtact
      721 cgaagctggt ggtctcctgg aaggtctccg ggctcggcca gaacctgatc gtggtgccgc
      781 tcttcttgat cgggcgcccc ttcttgggct ctttcgcctt gggctggccg tccctgaact
      841 cctgggtgta ggcaaacccg ccgcggcgca cttcggcctc gagacgagaa gagagggcgt
      901 tgaccaccga gaccccgacc ccgtgcagcc caccggagac cgtgtacgcg ccctgttcga
      961 acttcccgcc ggcatgcaag gtggtgagca cggtggtgag ggccgacttc ttcgacttct
     1021 cgacacgctc gaccgggatg ccgcggccgt catcgatgac ctcgacccct ccgtcggcgc
     1081 gcaaggtcac catgatcttg gtgcagaagc cggccattgc ctcgtccacc gagttgtcga
     1141 ccacctccca gatgagatgg tggagacccc ggggcccggt ggttccgatg tacatgccgg
     1201 gtcggcgttt caccgcctcg agaccgtcga gaacggtgat gtctttggct tcgtaactgt
     1261 cgcgggtggc cgtcacgacc ctcctttagg gacctggatg gaaggtggga tccgcctggc
     1321 tcgcggagac tttcacgtat gtcattttac cgatgcttac tgacaaaaac caatccatcc
     1381 gttatcggga gacctcagtt ccgcgtcggc ggcgtgacct cgatctgatc gacgactccc
     1441 tcgccgaaac gggccttcaa gctctccaac agctgggccg agccatacct cagaaaagcc
     1501 accatcgagg gcgaagacgc ctccaccacc agggttctcc cctggatgac aaccggtcgc
     1561 gacctcccca cccacgggtt cccagcgagc tcgtcccact cgtcgaccag ttgggacatc
     1621 agcaccgggt cgggca
//
LOCUS       DATITW010000104         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_85081, whole genome shotgun sequence.
ACCESSION   DATITW010000104 DATITW010000000
VERSION     DATITW010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85081"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VLS86_01475"
     CDS             complement(<1..737)
                     /locus_tag="VLS86_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSO49191.1"
                     /translation="MALKLPIFMDSQSTTPVDPRVLDAMIPYFTEKFGHPASRNHPFG
                     WEAEGAVDRARDEIAKLIGARDPKEVIFTSGGTESINLALKGVAEMYREKGTHIVTTT
                     IEQRASMDVGKRLERQGFEVTYVSVGPDGLVDPAELEKAITPKTILISVMFANNEIGT
                     IQPIAEIGKLAKARGIVFHTDATQAAGKVPIDVEAMGIDLLSATAHMMYGPKGIGALY
                     VRRKGPRVRISPMMDGGGHERGMRSGTV"
     gene            complement(771..1217)
                     /locus_tag="VLS86_01480"
     CDS             complement(771..1217)
                     /locus_tag="VLS86_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSO49192.1"
                     /translation="MLRFTKRADYGLMAMHFISSHGDEGVVSAKRIAEEFHIPPERLA
                     KILQRLAKKGLITSHNGPKGGYVLTREAGAITVGQVVRALEGPLRIVSCMSEKDDCPQ
                     FSRCNLRGPVQKIQTSISMVLDGMTLAELAADPGISVGAPALMSQR"
     gene            complement(1368..>2298)
                     /locus_tag="VLS86_01485"
     CDS             complement(1368..>2298)
                     /locus_tag="VLS86_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HSO49193.1"
                     /translation="PPPTAECPFCPGNESLTRPEISAYRKDGSPPDTPGWSVRVIPEI
                     DPYFGVEQELVLEGIGLYDRVSPRGATELIVESPGHGDAPATMREEQWEQVLWMYRER
                     VRDLKRDRHIRDILVTRRHRSPGSRITHPYSRLTAIPIIFDDVRRKLRQCRDYYQYKR
                     RCIYCDIVRQDLADGDRVIHATEHFAVLAPYAARSAFETWILPRRHGCSYEDALTEDV
                     VADLASLLRSYFRTLAGSLGDPPFEMTLYTAPNVGVKILSGEWSTIAEDYHWHLEVTL
                     DPYRRARIGGIYVNEMPPEEAAKTLRDAWPRGV"
BASE COUNT          365 a          764 c          730 g          439 t
ORIGIN      
        1 accgtacccg agcgcatgcc gcgctcgtgc ccgccgccgt ccatcatcgg cgagatgcgc
       61 acccgggggc cctttcgccg cacgtagagc gcgccaatcc ccttggggcc gtacatcatg
      121 tgggcggtcg cggaaagcag atcgattccc atcgcctcga cgtcgatcgg gaccttgccc
      181 gcggcctggg tggcatcggt gtggaagacg atacccctgg ccttggcgag cttgccgatc
      241 tcggcgatgg gctggatggt gccgatctcg ttattggcga acatgaccga gatcaggatg
      301 gtcttcggcg tgatggcctt ttcgagctcg gccgggtcga cgagtccgtc gggtcccacc
      361 gagacgtagg tgacctcgaa gccctggcgc tcgaggcgct tgccgacatc catgctcgcc
      421 cgctgctcga tcgtcgtggt gacgatgtga gtccccttct cgcggtacat ctcggccaca
      481 cccttgagcg ccaggttgat ggactccgtg cccccagagg tgaagatgac ctccttggga
      541 tcccgggcgc cgatcagctt ggcgatctcg tcgcgggcgc ggtccaccgc cccttccgct
      601 tcccagccga aggggtgatt gcggctcgcg gggtgcccga atttctccgt gaagtacgga
      661 atcatcgcgt cgaggacccg agggtccacc ggcgtcgtcg actgactgtc catgaagatc
      721 ggcagtttca gggccatcga ttctcccctc tccgggtcac tcgtatctga tcagcgctgg
      781 ctcatcaggg ccggcgcacc gaccgagatg cccgggtcgg ccgccagttc ggccagcgtc
      841 atgccatcga gcaccatgct gatgctcgtc tggatctttt gcaccggacc ccgcagattg
      901 cagcgcgaga actgcgggca gtcatctttc tccgacatgc agctcacgat tcggagcggc
      961 ccctcgaggg cccggaccac ctgcccgacc gtgatagctc ccgcttcacg cgtcagcacg
     1021 tagccgccct tgggaccgtt gtggctcgtg atgagcccct tcttggccaa gcgctggagg
     1081 atcttggcca ggcgctcggg cgggatgtgg aactcctccg cgatgcgctt ggcgctcacc
     1141 accccttcat cgccatggga cgagatgaag tgcatcgcca tcagtccgta gtcggcccgc
     1201 ttggtgaatc tgagcatcta tttgagttcg acctcgctgg tccggtctcc acctggccgg
     1261 taatcgctat atccgacctt cgtgatcgcc gacccttata gtcggagtct gccacccaca
     1321 ttgtcgcctg tcaactgaaa catgaggttc tacagtaatc tcgacgctta cacccctcgt
     1381 ggccaggcat cccggagtgt cttcgccgcc tcttcggggg gcatctcgtt gacgtagatg
     1441 cccccgatcc tggcccggcg atagggatcg agcgtcacct ccaggtgcca gtggtagtcc
     1501 tcggcaatgg tcgaccactc ccccgacagg atcttgacgc ccacattcgg agccgtgtag
     1561 agcgtcatct cgaacggggg gtcccccagc gaaccggcta gggtgcggaa atagcttctc
     1621 aagagggagg ccaggtccgc caccacgtcc tcggtcaggg cgtcctcgta ggagcagccg
     1681 tgccgcctcg gcaggatcca ggtctcgaac gccgacctcg ctgcatatgg cgcgaggacc
     1741 gcgaagtgct ccgtggcgtg gatgacccgg tcgccgtcgg ccagatcctg acggacaatg
     1801 tcgcagtaga tgcagcgccg cttgtactgg taatagtcac ggcactgccg cagcttcctg
     1861 cggacgtcgt cgaagatgat cgggatcgcg gtgaggcgcg agtacggatg cgtgatgcgg
     1921 gagcccggcg agcggtgccg ccgggtgacc aggatgtccc gaatatggcg atcacgcttc
     1981 aggtcgcgca cccgctcccg gtacatccag agcacctgct cccactgctc ctcgcgcatc
     2041 gtggcgggcg cgtcgccatg cccggggctc tccacgatca gctcggtggc cccgcgtggc
     2101 gagacccggt cgtagagccc gatgccctcc aggaccagct cctgctcgac accgaagtag
     2161 gggtcgatct ccgggatcac tcggaccgac catcccggcg tgtcgggtgg agaaccgtcc
     2221 ttgcgatagg cggagatctc cggtcgagtc aggctctcat tgcccgggca gaaggggcac
     2281 tcggcggtcg gcggtggc
//
LOCUS       DATITW010000105         1791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_205347, whole genome shotgun sequence.
ACCESSION   DATITW010000105 DATITW010000000
VERSION     DATITW010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205347"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..>1791)
                     /locus_tag="VLS86_01490"
     CDS             complement(26..>1791)
                     /locus_tag="VLS86_01490"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSO49194.1"
                     /translation="RIRRGDQTTRGDVVVEGFYEVGMQDQASLGTEAGLAVPDGQGGV
                     DLFATSQFVHVDQEQVVASLGLRPDQVRAHPTGIGGAFGSREDVNLHIHLCMLALHTG
                     RPVKMVYHRSESFAGHVHRHPARLRYRHEADRDGWLVRVEAKVVIDGGAYASTTSAVL
                     ANACYFAVGPYRCDSVAVDGVGVRTNNPPCGAMRGFGAVQVCFAYETQMDRLAHALGM
                     DPLVLREINALERGDPMPTTGQLIGTPLPTKEVIRSLRAMPLPDADPVPDRSRLPGGS
                     GLTTEQHEVTRGVGYAFGIKNLGFSEGFDDYAQARVRLPADGAVVETAAIEVGQGLVT
                     VLAQIARTALGITNVQVGHVDTSKIDSAGSTSASRQTQLSGGATLEAATRLRERVLSS
                     FGGDELDESGVWRGDELIAGWPELGDGDWSETVTFRHPPTDAPDADGQGSMHADFAVA
                     GHRAVVDVDVELGLVKVIRVDTVQDVGIALNPVSVRGQIEGGILQGVGLAIMEELILE
                     DGVIRNPTFTDYLLPTILDAPEVEALLIEQSGSWGPFGAKGVGEPPTISSTAAVVAAI
                     RVATGRELNRVPVRPQDIALP"
BASE COUNT          295 a          622 c          580 g          294 t
ORIGIN      
        1 gatcagatcg ctcatgtctg gtcgctcacg ggagagcgat gtcctgtggt cgcacgggga
       61 cccggttgag ctcccggccg gtggccaccc ggatcgcggc caccacagcg gcagtcgagg
      121 agatggtggg cggctcgcca acccctttcg ccccgaacgg gccccatgat ccgctctgct
      181 cgatgagcag cgcctcgacc tccggagcat cgaggatggt gggcagcagg tagtcggtga
      241 aggtcgggtt ccgaatcaca ccatcctcca ggatcagctc ctccatgatc gccagaccca
      301 ccccctgcag gatgccgcct tcgatctgac cacggaccga taccgggttc aaggcgatgc
      361 cgacatcctg gacggtgtcg acccgaatca ccttcaccag gccgagctcg acatcgacgt
      421 cgaccacggc ccggtggccg gcaactgcga agtcggcgtg catgctgccc tggccgtcgg
      481 cgtcgggtgc gtcggtcggt ggatggcgaa aggtcacggt ctccgaccag tcaccgtcac
      541 cgagctcggg ccacccggcg atcaactcgt ctccccgcca cacaccactc tcgtccagtt
      601 cgtcgcctcc gaagctcgag agcacccgct cccggagcct ggtggccgcc tccagggtcg
      661 ccccgcccga gagctgggtc tgcctcgagg cggaggtcga tcccgcagag tcgatcttcg
      721 aggtgtcgac gtggcccacc tgcacattgg tgatccccag cgcggtgcgg gcgatctggg
      781 ccaggaccgt caccaggccc tggccgacct cgatggcggc agtctcgacc acggccccat
      841 ctgcggggag ccggaccctg gcttgtgcgt agtcgtcgaa tccctccgag aaaccgaggt
      901 tcttgatccc aaaggcatag cccacccctc tcgtcacctc atgctgttcg gtggtgagcc
      961 cggagcctcc gggtagacgg ctgcgatcgg gaaccgggtc ggcgtcgggc aggggcatgg
     1021 cccgaagact gcggatgacc tccttggtgg ggaggggggt gccaatcagc tgaccggtgg
     1081 tcggcatcgg atcacctctc tccagggcgt tgatctccct gagaaccagg gggtccatgc
     1141 ccagggcgtg tgccagccgg tccatctgcg tctcgtaggc gaagcacacc tggaccgcgc
     1201 caaaaccgcg catcgcgccg cagggcgggt tgttggtccg gaccccgacg ccgtccaccg
     1261 ccaccgagtc gcatcgatag ggaccgaccg cgaagtagca ggcattggcc agcaccgcag
     1321 atgtcgtcga tgcgtacgcc ccgccgtcga tgaccacctt ggcctcgacc ctcaccagcc
     1381 agccatcgcg gtctgcctcg tggcggtagc gaagccgcgc cgggtggcgg tggacgtgcc
     1441 cggcgaagga ctcggaccgg tggtagacca tcttgaccgg tcgaccggtg tgcagggcca
     1501 gcatgcagag atggatgtga aggttcacgt cttccctgga cccaaatgct ccgccaatcc
     1561 ccgtgggatg ggcccgaacc tgatcagggc gaagaccgag gctggcgacc acctgctctt
     1621 ggtcgacgtg tacgaactga ctggtggcaa atagatcgac gccgccctgc ccatcgggga
     1681 ccgccaaccc ggcctcggtt ccgagggagg cctggtcctg catccccacc tcgtagaagc
     1741 cctcgaccac cacgtctcca cgcgtggtct ggtctcctct gcggatgcgc a
//
LOCUS       DATITW010000106         1583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_245454, whole genome shotgun sequence.
ACCESSION   DATITW010000106 DATITW010000000
VERSION     DATITW010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245454"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VLS86_01495"
     CDS             complement(<1..737)
                     /locus_tag="VLS86_01495"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HSO49195.1"
                     /translation="MSAEPTQPNSGRRGFFSRAARGASERVLDVVDPDMVLDHIDING
                     LLDRIDINALLDKVDVDRLLERIDIDELLQRADNDAVMARVDVRALVDRAGIPEIVAE
                     STSHLTGSALDMFRRPIVGIDQVTSTALNRLVRRDVRQFPTGPGALVSWVADQGDDDP
                     AIRTGRYAGPVTRLIAFVLDSLIVTTGFTVIVAGFTFLVELITARQVEVTEGVWFVAG
                     YVLWAFLYLWLSVAVFGKTLGKTVMGVR"
     gene            complement(747..>1583)
                     /locus_tag="VLS86_01500"
     CDS             complement(747..>1583)
                     /locus_tag="VLS86_01500"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSO49196.1"
                     /translation="LLAIAHEAGVELELEDFDRISRRTPHLGDLKPFGKYHMVDLDRV
                     GGVPLVLRALLDENLIDGDVLTVTGRTMAENLSDVRFDPTQGVIASPATPLAPEGGIA
                     ILRGNLAPEGAVVKMAGIDLDVFEGPARVFDGEQGAIDALFAHQIAHGDVVVIRTEGP
                     KGGPGMREMLQITAAIKGAGLGKDVLLITDGRFSGGTTGLCIGHVAPESEVGGPMGLV
                     EEGDRVRVDLPARRLDVMVDDAVLDERAARWTPHRPRYETGALAKYARLVGSAQSGAV
                     TN"
BASE COUNT          285 a          554 c          486 g          258 t
ORIGIN      
        1 cggaccccca tcacggtctt tcccagggtc ttgccaaaaa ccgcgaccga caaccagaga
       61 tagagaaacg cccagaggac gtatccggcc acgaaccaaa cgccctcggt cacctccacc
      121 tgacgggcag tgatcaactc gaccaggaac gtgaagccgg cgacgatcac ggtgaagccc
      181 gtggtgacga tgaggctgtc gagcacgaaa gcgatcagcc gggtcaccgg tcccgcataa
      241 cgtccggtgc ggatcgccgg gtcgtcgtca ccctggtcgg ccacccagct gaccagcgca
      301 ccgggcccgg tggggaactg acgtacatcg cgacggacga gccggttgag agcggtggac
      361 gtgacctggt cgatgccgac gatcggcctg cggaacatgt cgagcgccga tccggtgagg
      421 tgactggtgc tctcggcgac aatctcgggg atccccgccc gatcgaccag ggccctgaca
      481 tcgacccgcg ccatgacggc gtcgttgtcg gctcgctgca acaactcgtc gatgtcgatc
      541 cgttccaaga gccgatcgac gtccaccttg tccagcagcg cgttgatgtc gatccgatcg
      601 agaagtccgt tgatgtcgat gtggtccagc accatgtccg ggtcgaccac gtcgaggacc
      661 ctctcgctgg caccccgagc ggcacgggag aagaagcctc tacgacccga attcggctgc
      721 gtcggctctg cgctcacacc acgacgctag ttggttaccg ccccggattg cgcggaaccc
      781 accaaacgtg cgtacttggc cagtgccccc gtctcgtagc ggggccgatg cggcgtccat
      841 cgcgcggcgc gctcatccag cacggcgtcg tcgaccatga catcgagacg tcgggcggga
      901 aggtccaccc tcacccggtc accttcctcg acgaggccca tcggccctcc cacctccgac
      961 tcgggagcga catgtccgat gcagagaccg gtggtgccgc cggagaaccg cccatccgtg
     1021 atcaacagca cgtccttgcc cagaccggcg cccttgatcg ccgcggtgat ctgcagcatc
     1081 tctctcattc ccggcccgcc cttcggcccc tcggtgcgaa tcacgaccac gtcgccgtgg
     1141 gcgatctgat gggcgaacag agcgtcgatc gcaccctgtt cgccgtcgaa gacccgagcc
     1201 ggtccttcga acacgtcgag gtcgattccc gccatcttca ccaccgcacc ctcgggagcg
     1261 aggttgcccc gcaggatcgc gatcccgccc tcgggcgcca gcggggttgc cggtgaggcg
     1321 atgacgcctt gggtggggtc gaaccgcacg tcactcaggt tctcggccat ggtgcgaccg
     1381 gtcaccgtca gcacgtcgcc atcgatgagg ttctcgtcga gcaatgccct cagcaccagc
     1441 ggcactcccc cgacccggtc caggtcgacc atgtggtact tgccgaatgg cttgagatcg
     1501 ccgagatgag gggtgcgtcg tgagatgcgg tcgaaatcct ccagctcgag ctcgactccc
     1561 gcctcatggg cgatcgcaag cag
//
LOCUS       DATITW010000107         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_325718, whole genome shotgun sequence.
ACCESSION   DATITW010000107 DATITW010000000
VERSION     DATITW010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325718"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VLS86_01505"
     CDS             <1..647
                     /locus_tag="VLS86_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HSO49197.1"
                     /translation="ATSERFPVSGSRLPSRGGGVEAGRLIVGEVDVAVADLVSADPIH
                     LVNLATASALALRLGGTPSGIGKAARVFAPGGHRRTMVTVSGDVTWIDDSKATNPHAA
                     IASIRSHESVILIAGGLAKGLDLRPLAEEPNLRAVIGIGAEGPVLVEAAGSRGYLAGT
                     LDRAVELAATLAEPGDTVLLAPGCASFDQFKSYAERGDQFASLVREKTGGGSAG"
     gene            644..1807
                     /gene="ftsW"
                     /locus_tag="VLS86_01510"
     CDS             644..1807
                     /gene="ftsW"
                     /locus_tag="VLS86_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HSO49198.1"
                     /translation="MTANVTSIAKMRAAARDRAERIKVNNRTATTLLVLVVVLVVIGL
                     GATQSASSAVAIEQAEDRFYFFKRQLVGVGIGTVALLVASRIPYQFYRKIAIPFFVAT
                     VALLVAVLAVGPVIGGAQRWLPVAGFSFQPSELAKVAVVFALAYLLEKKHKLVDRFGH
                     FLVPVVFIVGTIAVLVMKQPDLGTVIVIGAAALAVILASDAPLRYVAFLAIAAILIVS
                     VLAFSDDYRADRIVGFLDPWGTSSGEGYQLVQSYYALSNGGIFGVGLGASRARWFYLP
                     NAHTDFIFAIIGEETGLIGALTVIGLFVVLALAGWMVALRAPDRFGRMVAAGITVWLS
                     FQALVNIGGVLGVLPITGIALPFISFGSTALIASMAALGVLVNIAQAGVHRKT"
     gene            1804..>2026
                     /locus_tag="VLS86_01515"
     CDS             1804..>2026
                     /locus_tag="VLS86_01515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSO49199.1"
                     /translation="MSFLIAAAGTGGHVFPGLAVGEALIDLGVPKEDVVFVGGDRMEA
                     EVYPRAGFSFLQVEMRGLRRSASAKNLGLP"
BASE COUNT          301 a          628 c          676 g          421 t
ORIGIN      
        1 gtgcgaccag tgagaggttc ccggtttcgg gctcacgtct tccatcgcgc gggggaggtg
       61 tcgaagctgg gcgtctcata gtcggcgaag tggatgtggc cgttgccgac ctggtttcgg
      121 ccgatccgat ccatctcgtc aacctggcga ccgcttcggc gcttgcgctg cgactgggag
      181 gcactccatc tgggatcggc aaagcggccc gagtcttcgc tccagggggc caccgtcgca
      241 caatggtgac ggtcagtggc gatgtgacct ggatcgatga ctccaaggcg accaacccgc
      301 atgccgcgat tgcctcaatt cgctcccatg agtctgtgat cctgatcgcc ggaggacttg
      361 ccaagggcct cgacctgaga cctctcgccg aggagccaaa tctgcgggcg gtcatcggga
      421 tcggtgccga gggcccggtc ttggtggaag cagcaggatc ccggggatat ctcgccggga
      481 ccctggaccg agcggtcgag ttggcggcca ccctcgccga gccgggggac acggtcctcc
      541 tggcgccggg atgcgccagc ttcgatcaat tcaagtcgta tgcagagcgg ggcgaccagt
      601 tcgcctcctt ggttcgagag aagacaggag gaggctcagc aggatgacgg cgaatgtgac
      661 ttcgatcgcc aagatgaggg cggccgcccg cgaccgtgcc gagcggatca aggtcaacaa
      721 ccgcaccgcg accacattgc tggttctcgt cgtggtcctg gtggtgatcg ggctcggggc
      781 aactcaatcc gcctcgtctg cggtagccat cgagcaagcc gaggaccggt tctacttctt
      841 caagcgtcag ctggtcgggg tggggatcgg gacggtcgcc ctgctcgtgg caagccggat
      901 tccctatcag ttctatcgca agatcgccat ccccttcttc gtggccaccg ttgccctgct
      961 ggtcgcggtg ctcgcagtcg gtccggtgat cgggggagcc cagcgttggc tgccggtggc
     1021 cggcttcagc ttccagccat cggagctggc gaaggtggcg gtggtcttcg cccttgccta
     1081 tctcctcgag aagaagcaca agctggtcga tcgtttcggc cacttcctgg tccccgtggt
     1141 cttcatcgtg gggacgatcg cggtgctggt gatgaaacaa ccggacctgg gcacggtgat
     1201 cgtgattgga gcggccgccc tcgcggtgat cctcgccagt gacgcacccc ttcgctatgt
     1261 cgcctttctc gctatcgcgg caatcctcat cgtttcggtg ctcgccttca gcgacgatta
     1321 ccgggccgac cggatcgttg gcttcctcga tccgtggggc acctcgagcg gcgagggcta
     1381 ccagctggtc cagagctact acgccctgtc caatggcggg atcttcgggg tcgggttggg
     1441 ggcgagccgc gctcggtggt tctatctgcc caacgcccac accgacttca tctttgcgat
     1501 catcggtgaa gagaccggtt tgatcggggc cttgaccgtg atcgggctct tcgtggtgct
     1561 cgcccttgcc ggttggatgg tcgccttgag agcgcctgat cgattcggga ggatggtcgc
     1621 cgccgggatc acggtttggc tctcgttcca ggccctggtc aatatcgggg gcgtcctcgg
     1681 cgtgcttccg ataacgggga tcgcgcttcc cttcatctcg ttcggcagca ccgccctcat
     1741 cgcctcgatg gcggcgctcg gcgtcctggt gaacatcgcc caggctggag tacatcgcaa
     1801 gacatgagct ttctgatcgc ggccgctggc accggtgggc atgtgttccc gggtctcgcg
     1861 gttggcgaag ctctgatcga cctcggggtc cccaaagaag acgttgtctt tgtcggtggc
     1921 gaccggatgg aggccgaggt ctatccccga gccgggtttt cgttcctcca agtcgagatg
     1981 cgcggcctgc gacgctcggc ctcggcgaag aacctgggtc tgcccc
//
LOCUS       DATITW010000108         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_5235, whole genome shotgun sequence.
ACCESSION   DATITW010000108 DATITW010000000
VERSION     DATITW010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5235"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..983)
                     /locus_tag="VLS86_01520"
     CDS             complement(291..983)
                     /locus_tag="VLS86_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSO49200.1"
                     /translation="MTLPDLAIPGVRRGIEEMLVRRAQVLAQGAKPIGWKLGFGAPAW
                     LDKFGLTGPLVGFLTDATRYPSGAAVSCEGWSNPVAEPEIAVHIGPEAVDPDHAGAAI
                     DVIGPAIELADVHSPPEDIAEVLAGNIFHRALVLGETASPRAGGVLAGLRARIVCDGV
                     EVADTTDLEVLTGGLVAILGHAATLLASAGEAIRPGEVVIMGSVIPPQPVRPGQVVSF
                     ELSPISTVSVSV"
     gene            complement(980..1384)
                     /locus_tag="VLS86_01525"
     CDS             complement(980..1384)
                     /locus_tag="VLS86_01525"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSO49201.1"
                     /translation="MDPFRTSLDAVPLVPGLRTDEGWVDMRVQFLVDQQTAGSDDFLL
                     GWTVLPPGARHDRHRHHNAEEFFLVLQGAGMVFTDRGREPCGQGDVVFTPRGHWHGFD
                     NTGDEDVVLVWGWSGAGSLEGSGYEAESPESS"
     gene            complement(1384..2265)
                     /locus_tag="VLS86_01530"
     CDS             complement(1384..2265)
                     /locus_tag="VLS86_01530"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSO49202.1"
                     /translation="MDLGFIGLGDMGQVIVQRLLDAGHRVTGWNRTAAKAAPLLARGM
                     TWADTPRQVAALSEVVFSVVTDAAAVRAVALGEDGVIAGLPADSIYLDMSTIDPDVSR
                     VIAAEFAAANLTMLDAPLSGSPITVANGTASTMVGGDRAAFDRVAPVLYDIGSKVSYI
                     GVTGSAVQMKLAINLSLIVEMVAFCESVALAEKGGVDRAVAVDAMLKSVVASPVMAYR
                     GPFILEGNMPDKPLADVTLQQKDMLLALEVARRQGSPAPLAAVANEMLNACRGRGIDH
                     RDFVTVFDVYHLLSGMS"
     gene            complement(2402..>2985)
                     /locus_tag="VLS86_01535"
     CDS             complement(2402..>2985)
                     /locus_tag="VLS86_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HSO49203.1"
                     /translation="GIHLIMATQRPSVDVITGVIKANVPSRLAFSVASQTDSRVILDG
                     SGAEKLIGMGDMLVVTAKEPRAQRIQGAWVRESEIHAVVEWVRTQQEAKYEEQEVLQA
                     AAGAAAANAEEVDDEEAALVRQAMELVVRSELGSTSMLQRKLRIGFARAGRVMDILES
                     RGVVGPSHGSKAREVLMTVEEMEDALAAEGAGV"
BASE COUNT          482 a         1065 c          944 g          494 t
ORIGIN      
        1 accctccgct tcgggggccg tggtgtccga tggggtcgtc gtctctccgg tgtcgggggc
       61 ggtggtggtt gcaccctctc cggccgtggt cgtcgtgtct gccgtttcac cacacgctga
      121 tgccaccaac gccaccacgg cgagggtcgc gaccaaccgc ttccaagatt tcatgccctg
      181 ctcctcctca gctcatcctc ccactgaatg cgggcggcaa acgattgtat gccgtggccg
      241 ccgagcgcgt caacagaccg tcctgcaatc gattgcttac cgtagaacag ctagaccgac
      301 acggagaccg tcgagatcgg cgacagctcg aaactcacca cttgccccgg cctcaccggc
      361 tgtgggggga tcaccgagcc catgatgacc acctcgccag gtcggattgc ctcgccggcg
      421 ctggcgagca gggtggccgc atgaccgagg atggccacca agccgccggt caggacctcg
      481 aggtcggtgg tatcggcaac ctcgaccccg tcgcacacga tccgggcacg gagaccggca
      541 aggaccccgc cggctcgggg cgacgcagtc tcaccgagca ccaatgcccg gtggaagatg
      601 ttcccggcca gcacctccgc gatgtcctcg ggtggggagt ggacatcggc gagctcgatc
      661 gcgggcccta tgacgtcgat cgccgccccg gcatgatccg ggtccacagc ctccggtccg
      721 atgtggaccg cgatctcggg ctcggccacc gggttgctcc acccctcgca cgacacggcg
      781 gcgcccgacg ggtatctcgt cgcatcggtg aggaagccga ccagaggtcc ggtcagcccg
      841 aacttgtcga gccaggccgg ggccccgaaa cccagcttcc aaccaatcgg cttggcacct
      901 tgggccagga cctgcgctcg tcgcaccagc atctcctcga ttccgcgtcg cacacccggg
      961 atggcaagat cgggaagggt cacgacgatt ccgggctctc cgcctcgtat cccgagccct
     1021 cgagtgagcc ggcgccactc catccccaga ccaggaccac gtcctcatcg ccggtgttgt
     1081 cgaagccgtg ccagtgcccg cggggggtga agaccacatc gccctggccg cacggctcgc
     1141 gacctcggtc ggtgaagacc atgccggcgc cctggaggac gaggaagaac tcctcggcgt
     1201 tgtggtgccg gtgtcgatcg tgtctcgccc cgggcggaag caccgtccac cccaggagga
     1261 agtcatcaga gcccgccgtc tgctgatcca ccaggaactg cacccgcatg tcgacccagc
     1321 cctcatcggt cctcagaccc gggaccaggg gcaccgcgtc gagacttgtg cggaaggggt
     1381 ccatcaggac atcccgctca gcaggtgata cacgtcgaac acggtgacga agtcccggtg
     1441 gtcgattccc cgtccccggc atgcattgag catctcgttg gcaactgcag ccaacggcgc
     1501 cggcgagccc tgacgacggg caacttccag cgcgagaagc atgtcctttt gctgcagggt
     1561 gacgtccgcc agcggcttgt cgggcatgtt cccctcgagg atgaacggcc cccgatacgc
     1621 catgaccggt gaggcgacga cgctcttgag catggcatcg actgcgactg cccggtcgac
     1681 ccctcccttc tcggccaggg ccaccgactc gcaaaaggcc accatctcca cgatcaggct
     1741 caggttgatg gccagcttca tctggaccgc gctgccggtc actccgatgt agctcacttt
     1801 ggacccgatg tcgtagagca ccggcgccac ccggtcgaat gccgcccgat ctccgcccac
     1861 catcgtcgac gccgtcccgt tggccacggt tatcggactt cccgacaagg gggcgtcgag
     1921 catggtgaga ttggctgcgg cgaactcggc tgcgattacg cggctgacat cgggatcgat
     1981 ggtgctcatg tcgaggtata tcgaatccgc ggggagcccg gcgatcacac cgtcttcccc
     2041 cagggccacc gcccgcaccg ccgcggcatc ggtgacgacg gagaacacca cttcggagag
     2101 cgccgccacc tggcgcgggg tgtcggccca cgtcatgcct ctggcgagga gtggggcggc
     2161 cttggccgcg gtgcgattcc acccagtgac ccggtgcccg gcgtcgagca gtctctgcac
     2221 gatgacctgg cccatgtcgc cgagaccgat gaaaccgagg tccattgcct cctactcctc
     2281 ttgggccgga gactgtaacg gggcgtgggc aggctcagcg gcgggcgacg aagcccaagc
     2341 caagtgtcat caagagcggg ccggaggccg gtgacggcct ggcctcagtc gggtgacgga
     2401 gtcagactcc cgccccctcg gcggcgagag catcctccat ctcctccacc gtcatcaaga
     2461 cttcccgggc cttgctcccg tgtgagggcc cgaccactcc cctgctctcg aggatgtcca
     2521 tcacccggcc ggcgcgggcg aacccgatcc ggagcttgcg ttgcagcatc gacgtggagc
     2581 cgagctcgct gcgcaccacc agctccatcg cctgacggac gagggcggct tcctcgtcgt
     2641 cgacctcttc ggcgttggcg gctgccgccc cggccgccgc ctggaggacc tcctgctctt
     2701 cgtacttggc ctcctgctgg gtgcggaccc actcgaccac cgcgtggatc tcgctctccc
     2761 gcacccatgc tccctggatt cgctgcgccc tgggctcctt ggcggtgacc accagcatgt
     2821 cgcccatgcc gatcagcttc tctgcgcccg aaccgtcgag gatgacccga ctgtcggtct
     2881 gcgaggcgac gctgaaggcg agacggctcg gcacgttggc cttgatcacg ccggtgatga
     2941 cgtcgaccga tgggcgctgg gtcgccatga tcaggtggat gccgg
//
LOCUS       DATITW010000109         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_726209, whole genome shotgun sequence.
ACCESSION   DATITW010000109 DATITW010000000
VERSION     DATITW010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726209"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VLS86_01540"
     CDS             complement(<1..1259)
                     /locus_tag="VLS86_01540"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HSO49204.1"
                     /translation="MRTSTRDIGTPQRDPGQQDLHRVFISYSRSDFYFAEQLAGALRR
                     RGFAVWFDVHELGPGTDWSSAIDRAIAECDTFLVVASRAALESPYVQQERDRAVELTR
                     SCIAVLPRRLGSADIPTGPTYDLASSFRRGVDEIAADLTAGRPRGWRPRLPLPFPASV
                     LLVALAPVLATLLAVVMGTSFARAVAGQLIAFIPNGTWAVAVAVSMIALVGVYSAYCS
                     WAFIMRRSSWLNVRGFQFTMPLIALLAVVTVDTMAGYINEPWIFALGGEAEETYLGEA
                     SSVLAVGVVLASAAAAIITSYAAGPCRHLKTGIAPERVRRRHIGPVPGSVEIRPPARS
                     YRLIAAEDDSPVADEVRRALAGVGMAEVGDDAWSDRQIVVTSDRTPTEWLARDELKEP
                     LAVVATSIDLPVRGVLQRFQWVDYRRRR"
     gene            1771..2574
                     /locus_tag="VLS86_01545"
     CDS             1771..2574
                     /locus_tag="VLS86_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO49205.1"
                     /translation="MTDPDRQSLQAGWNQIDLNGARQWVSLRGDKDAPILLFLHGGPG
                     GAEYGPRRHYLAALEETWWVVDWDQRGAGRSFRGDETADSLSIDVLVGDGLALVDRLR
                     AEMPGRPIVVAGHSFGTVLGVMMASRAPGRIDAYVGASQVVNWALQESRSYQWALAQA
                     HRSGNEKATAALQAIGPPEGGVYPGGRASVDVQRRWLGTLGGVAGDPRFLMRWTMSIL
                     LARDYPMGAKLRFTKGLARSMDLVWPELGERIVSERSHHPRSPPPHLRR"
BASE COUNT          510 a          878 c          891 g          453 t
ORIGIN      
        1 cggcgacgtc ggtagtcgac ccactggaaa cgttgcagca caccgcgcac cggcaggtcg
       61 atgctcgtcg ccaccacggc gaggggctcc ttgagctcgt cgcgggcgag ccactcggtc
      121 ggtgtcctgt cggacgtcac cacgatctga cgatcgctcc aagcgtcatc gcccacctcg
      181 gccatcccga caccagccag ggcgcggcgg acctcgtcgg ccaccggcga gtcgtcctcg
      241 gccgcgatca accggtagga gcgggcgggg ggacgtatct ccaccgagcc cgggaccggg
      301 ccgatgtggc ggcgccggac acgttcaggg gcaatccccg tcttcaggtg gcggcacggc
      361 cccgctgcgt acgaagtgat gatggcggcc gccgcactcg ccagcacgac accgaccgcc
      421 aggacggagg acgcttctcc caggtaggtc tcctccgctt ccccgcccag cgcaaagatc
      481 cacggctcgt tgatgtatcc cgccatcgtg tcgacggtca ccacggccaa caacgcgatc
      541 aatggcatcg tgaactgaaa tccacggaca ttgagccagg acgagcgtcg catgatgaaa
      601 gcccaagagc agtatgcgga atagacgccg acgagcgcga tcatcgacac ggcgactgcc
      661 acggcccagg tgccgttggg tatgaaagcg atcagctggc cggcaaccgc ccgggcaaac
      721 gacgtgccca tcaccactgc gagcagtgtc gccaacaccg gcgccaacgc caccaggagc
      781 accgaggcgg gaaacgggag tggaagccgg ggacgccatc cccgtgggcg cccggctgtc
      841 aggtcggccg cgatctcgtc gaccccccgt ctgaaggagc tggcgagatc gtaggtcgga
      901 cctgtcggga tgtcggcgga gcccagtcgc cggggcagga ctgcgatgca gcttcgagtc
      961 agctcgactg cccggtctcg ttcctgctgc acatagggcg attccagcgc ggcccgggac
     1021 gcaacgacga gaaaggtgtc gcattcggcg atggcccggt cgatcgccga agaccagtct
     1081 gtgcctggcc cgagctcgtg cacgtcgaac caaaccgcga agccgcgccg acgcagggca
     1141 ccggccaact gctcggcgaa gtagaagtcg ctccgcgagt agctgatgaa aacgcggtgg
     1201 aggtcctgct gacctggatc cctctgcggc gtccctatgt cacgggtcga agtcctcaca
     1261 gcgccgcctc acggtcgagc cacgcacaac tgccagccag gtatgggccg aatgaaacgc
     1321 ggacgcggtc cacgacttac cccttgatcg ggcgatcaga cgatactccg atgcacaata
     1381 ccgaaaaccc caagtgctac ctggggattc tcggggtgga gccgagggga tttgaacctt
     1441 tgaccccttg ccgcgagtca tctcagcatg ccaagaaggc caacagccac cccggacgtt
     1501 gctcccccga tgcgctacga agcatgtctg ttccggcgtt tcggaccgat tctctgtcag
     1561 aagcgtttca catcctggat cgattcctga gagcgaagta ccaagacgtc acttagaacc
     1621 agtcagtgac cgagcatgaa ggctgggtcg caggctcaca gggtcaaaac gtcctgatcg
     1681 tgaccgctga ttcaggcgaa ttcgccatga cgcctggtga ctttgggccc taggcgcagg
     1741 tcacgtcctc catggacaat ccaacacatg gtgaccgatc cagataggca gtcgctgcag
     1801 gccggatgga accagatcga cctcaacggc gcccgccagt gggtctcact tcgtggcgat
     1861 aaagacgcgc cgatcctcct gttcctccac ggcggtcccg gtggggccga atacggtccg
     1921 agacgtcact acctcgctgc cctggaggag acgtggtggg tggtcgactg ggaccagcga
     1981 ggagcaggac gctcgttccg cggggatgag acagctgact cgctttcgat cgatgtcctg
     2041 gtgggagatg ggctggccct ggtggaccgg ctcagggcag aaatgcccgg acgcccgatc
     2101 gtggtggccg ggcactcctt tggcactgtg cttggtgtca tgatggccag tcgggcgccg
     2161 ggacggatcg atgcctatgt cggcgcctcc caggtggtga actgggctct ccaggagagc
     2221 cgttcctacc agtgggccct ggcccaggca catcgctcag gaaacgagaa agcaactgcc
     2281 gctctccagg cgatcggacc gcctgaaggc ggggtctatc cagggggcag ggcctcggtc
     2341 gatgtgcagc gtcggtggct cgggacgctg ggcggcgtcg ccggcgaccc ccgattcctc
     2401 atgcgatgga cgatgagcat cttgctggct cgtgactacc ccatgggtgc caagcttcgc
     2461 ttcaccaagg ggctggcccg ttcgatggac ctggtctggc cggagctcgg tgagcgcatc
     2521 gtttcggaga gaagtcacca ccctcggagt ccccctccac atcttcgccg gtgaccagga
     2581 tcggatcacc gacttggatc aggttcggga ttggctcgct gccctcgagg ctccgaccaa
     2641 gcggctcgag gtggtggagg gtgtcggaca tctgaatctg ttcgaggctc cgcaccggtt
     2701 cgtggaattc atgtcggggg tgcgacaagc ta
//
LOCUS       DATITW010000110         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_686287, whole genome shotgun sequence.
ACCESSION   DATITW010000110 DATITW010000000
VERSION     DATITW010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686287"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..136
                     /locus_tag="VLS86_01550"
     CDS             11..136
                     /locus_tag="VLS86_01550"
                     /inference="COORDINATES: protein motif:HMM:NF022219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49206.1"
                     /translation="MQASLWSQQMTAQTQLAEMQKRALLPPGETPPAEDASGLYL"
     gene            complement(183..458)
                     /locus_tag="VLS86_01555"
     CDS             complement(183..458)
                     /locus_tag="VLS86_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HSO49207.1"
                     /translation="MKSKLIEALAVGELSWQDYANCRGADADLFFPERGASTRKAKAI
                     CASCEVKVECLDYAVEMGEKFGIWGGLSERERRRVRRERAVRERKVG"
     gene            599..1327
                     /locus_tag="VLS86_01560"
     CDS             599..1327
                     /locus_tag="VLS86_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HSO49208.1"
                     /translation="MATTLEKRTLPWPLGFYRSAIGKKWVMAITGIAIILFVLAHMVG
                     NWKIFLPDVDGIPDIDIYAEALRQLLVPFAPEHVVLWLLRTGLIVAFLLHIHAAYSLT
                     LMNRRARPDDYQGPRNYVAANYASRTMRWSGTIFGAFLLFHLADLTWGIQPMAPETWE
                     RGEVYANFVATFSRAPVAIFYVVAMVLLGLHLYHGAWSMFQSLGINHPRFNAARRVFA
                     VGLAVLVTVGNAIMPLAVLFGFVG"
     gene            1332..>1527
                     /locus_tag="VLS86_01565"
     CDS             1332..>1527
                     /locus_tag="VLS86_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HSO49209.1"
                     /translation="MTVQLDARIPPGPIADKWENHKFSIKLVNPANQRKFKILIVGTG
                     LAGASAAATLAELGYEVEVFT"
BASE COUNT          275 a          525 c          430 g          297 t
ORIGIN      
        1 gttccacggg atccaggcgt cgctgtggtc acagcagatg acagcccaga cccagttggc
       61 ggagatgcag aaacgggctc tgctcccacc gggggagacg ccaccggccg aagacgcctc
      121 cggtctgtac ctttagtcct attacctagt accagtaccc ggtaaggcgg cgctgggcgc
      181 ccctacccaa ctttgcgttc gcggaccgcc cgctcccgcc gcacccgtcg acgctctcgt
      241 tcgctgagac caccccagat gccgaacttc tcacccatct cgaccgcata gtcgagacac
      301 tccaccttca cctcgcacga ggcacagatt gccttcgcct ttcgggtgga ggctcccctt
      361 tcggggaaga agaggtccgc atccgccccc cggcaattgg cgtagtcctg ccacgaaagc
      421 tcgcccaccg ctagcgcttc gatgagcttg ctcttcatca ggtctcctca caaacgtgta
      481 attacacatt tgagattacg actacccgcg cgcgccgtca agggcgcgct ctgagaagcc
      541 gtcgaccaca ggcggtcggg gattggaccg gacgggagag ttgccataat gagcagccgt
      601 ggcgaccacg ctcgagaaga gaacactccc ctggcccctg ggcttctatc gatcggcgat
      661 tggcaagaaa tgggtcatgg cgatcaccgg aatcgccatc atcctcttcg tgctggccca
      721 catggtgggc aactggaaga tcttcctccc cgacgtcgac ggcatccccg acatcgacat
      781 ctatgccgaa gcactgcgtc agttgctggt ccccttcgcc cccgagcacg tggtcctttg
      841 gctgctgcgc accgggctga tcgtcgcctt cctactccac atccatgccg cctactccct
      901 gaccctgatg aaccggcgcg ctcgacccga cgactatcag ggaccccgca actacgtcgc
      961 cgccaattac gccagtcgca ccatgcgttg gagcggcacc atcttcggag ccttcctgtt
     1021 gttccatctc gccgacctca cctggggcat ccagccgatg gcgcccgaga cctgggaacg
     1081 aggtgaggtg tacgccaact tcgtcgccac cttcagccgg gcaccggtgg ccatcttcta
     1141 tgtggtggcg atggtgttgc tcggtctcca tctctatcac ggtgcgtggt cgatgttcca
     1201 gagtctcggg atcaaccacc ctcgattcaa cgcggcacgc cgggtgttcg ccgtcgggct
     1261 tgccgtgttg gtgacggtcg gcaacgccat catgcctctc gccgtgctct ttggtttcgt
     1321 gggatgagga gatgacagtc caactcgacg ccaggatccc gccggggccg atcgccgata
     1381 agtgggagaa ccacaagttc tcgatcaaac tggtcaaccc ggccaaccag aggaagttca
     1441 agatcctcat cgtgggaacc ggcctcgccg gcgcctcggc cgctgcgacg ctcgccgagc
     1501 tcggctacga ggtcgaggtg ttcacct
//
LOCUS       DATITW010000111         1764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_365714, whole genome shotgun sequence.
ACCESSION   DATITW010000111 DATITW010000000
VERSION     DATITW010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365714"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VLS86_01570"
     CDS             <1..860
                     /locus_tag="VLS86_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49210.1"
                     /translation="RRIDGGTVPFFYAWSPEGDHVAYLGNSPDGAGVAMGLVDVAAGT
                     ARLVDSGAPYYLDWAPDGSRLAVHVDSTFLGFVDLEGNRTPITTVAGLFQAPQFLNDG
                     RLLVAAGGDLQGVTLAGDSSEPVLIAPITGGTFFSASANGAMLAFTDTANAPILGSLA
                     VVPIEGGEPQSVNEDGVVAFEWSPSGEQLLFMTVNAEGTALIPQLWEAGSVEMYEELL
                     PTQEMISSYLPFWDQYSRVLTLWAPDGASFVLPVNGENGGSIVVYDVESGATTRVSGG
                     RFASWAPGS"
     gene            915..>1764
                     /locus_tag="VLS86_01575"
     CDS             915..>1764
                     /locus_tag="VLS86_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49211.1"
                     /translation="MVAVTITPEILWTLPRVGAPVTAGGGRVVVPVTTYDITANRGSS
                     QIWLVDGNGSRKALTAPEFNASKPAVDPTGTRLAFVASIGEDKVKQIYISDVDALAEP
                     GSTKALTSFPLGALGGRWLPDGRGLVALAYLLKGHLDLGATATELARRKDAKFVVHTT
                     EDLIYRYWDTWLTTGEVPHLFRVDLHSGKTTDLTPGSTRWWSWPNTDDPLDSFAVSPD
                     GRHIAYVADSSEPPHRRIRRHLYELDLETGHEADLTPEGVVQVHRPRYSPDGRAIAYG
                     FQIVPDY"
BASE COUNT          310 a          583 c          542 g          329 t
ORIGIN      
        1 aaagaaggat tgacggcggc actgttccct tcttctacgc ctggagcccc gaaggcgacc
       61 acgttgccta tctcggcaac tcccccgacg gtgccggagt ggccatgggt ctggtcgacg
      121 tcgcggcggg cactgcccgc ctcgttgaca gcggggcccc ctattacctg gattgggcac
      181 cggacggctc gcgcctggcg gtgcatgtgg attcgacatt tctcggcttc gtcgacctcg
      241 aagggaacag gacgccaatc acgactgtgg ccggcttgtt ccaggcacca cagttcctta
      301 acgacggccg tttgctggtg gcagcggggg gcgacctcca gggcgtgacc ttggccggag
      361 actccagtga accggtgctg atcgctccca tcacgggagg caccttcttt tccgcttccg
      421 ccaacggagc catgctcgcc ttcacggata ccgccaatgc gccgatcctc gggtccctcg
      481 cggtcgttcc gatcgagggg ggagaaccac agtcggtcaa tgaggacggc gtcgttgcct
      541 tcgagtggtc cccatccggc gaacaactcc tcttcatgac cgtgaacgca gaaggcaccg
      601 ccctgatccc ccaactctgg gaggcgggct cggtggagat gtacgaggaa ctgcttccca
      661 cccaggagat gatcagctcg tatctcccct tctgggatca gtacagccgg gtcctgaccc
      721 tgtgggcacc ggacggtgcc agcttcgtcc tgccggtcaa cggcgagaac ggcggcagca
      781 tcgtggtcta tgacgtcgag agcggcgcca ccacccgggt gtccggcggt cgcttcgcca
      841 gctgggctcc cggttcctag ctagagaccg aacttttcgg atcgggcgct cgggatcgag
      901 ccgttatggt cccgatggtg gcggtgacta tcacacccga aatcttgtgg acccttcccc
      961 gggtcggggc accggtgacg gcgggcggcg gtcgcgtggt tgtgccggtg accacctacg
     1021 acatcaccgc caacaggggc agcagtcaaa tctggttggt cgacggcaat ggttcccgaa
     1081 aggccctcac cgcccccgaa ttcaatgcct ccaaaccagc tgtggacccg accggtacca
     1141 gactcgcgtt tgtcgcttcg atcggtgagg acaaggtcaa gcagatctac atctcggatg
     1201 tggacgcgct cgccgagccc ggttcgacca aagcattgac ctcgttcccg ctaggagccc
     1261 ttggtggtcg atggctgcct gatggcagag gactcgtggc attggcgtac ctgctcaagg
     1321 gacatctgga tctcggagca acggccaccg aacttgctcg acggaaggat gcgaagttcg
     1381 tcgtgcacac caccgaagac ctcatctatc ggtactggga tacatggttg acgacgggcg
     1441 aagtgccgca tttgttccgc gtcgacctgc atagcggcaa gacgaccgat ctcactccgg
     1501 gctcgacccg atggtggagc tggccgaaca ccgacgaccc gctcgacagc ttcgccgtct
     1561 cccccgacgg ccgtcacatc gcctacgtgg ccgattcgtc cgagcctccc caccgcagga
     1621 tccggcgcca cctctacgag ctcgacctcg agaccggaca cgaagcggac ctcactccgg
     1681 aaggggtggt tcaggttcac cggccccgct actcacccga cggccgcgcg atcgcctatg
     1741 ggttccagat cgtccccgat tatt
//
LOCUS       DATITW010000112         1797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_165380, whole genome shotgun sequence.
ACCESSION   DATITW010000112 DATITW010000000
VERSION     DATITW010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165380"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VLS86_01580"
     CDS             complement(<1..316)
                     /locus_tag="VLS86_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014154700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSO49212.1"
                     /translation="MFTIGEFVALTGVSAKKLRHYDAIGLFRPAWVDPRSSYRYYLAT
                     QIPQLQRIVALRDLGIPLATIARLVDGGDSLEEELRRRRREILEERRSLDARLAALEI
                     RID"
     gene            369..1184
                     /locus_tag="VLS86_01585"
     CDS             369..1184
                     /locus_tag="VLS86_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014689471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSO49213.1"
                     /translation="MPEQNLRRPIRVAAQLHPQHGEYPRLHATVDQAEEMGFDLLYTW
                     DHFYPLYGERNGAHFECWTMLAAWAEQTTTIELGALVTCNSYRNPDLLADMARTVDHI
                     SGGRLVFGIGAGWFRRDFEEYGYEFGTAGSRLESLAGAIPRIKTRWEKLNPPPVRRPP
                     ILIGGGGLRKTLRMVAEHADSWHAGFPDDPAELEPKVSALLGWCDQLGRDPAEIEWGL
                     GVEPEDLDRFLSDDVASYVEMGFTQFTLGFQGPDWDLGPGGDWLAWRDRENLR"
     gene            1181..>1797
                     /locus_tag="VLS86_01590"
     CDS             1181..>1797
                     /locus_tag="VLS86_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSO49214.1"
                     /translation="MTEARERGHQIRFGVVTPQMWHSWGEMVELWSRIEGAGWDAAFV
                     VDHFLSDWEGEMGAQLEAFTLLAGLAREVPRIDIGTFVAGITHRRPAVLAKQAVTLDH
                     VSDGRFIFGVGAGWNEREHEAYGIPFPGAADRVSQVGEALEAIRLLEANTTTDFTGET
                     LRLNQAPFEPKPVNGRLRVLVGSRRPKMLGHLARFGDLWDAAGSL"
BASE COUNT          302 a          594 c          603 g          298 t
ORIGIN      
        1 gatcgatcct gatctccagc gccgcgagcc gggcgtcgag acttcgtcgc tcctccagga
       61 tctcccggcg ccgacgtctc aactcctcct ccagggagtc tccgccgtcg acgagccggg
      121 cgatggtggc cagggggatc cccagatcac gaagggcgac gatccgttgc agctgtggga
      181 tctgggtcgc caggtagtag cggtaggacg accggggatc gacccaggcc ggtcggaaca
      241 aaccgatggc gtcgtagtga cggagcttct tcgccgagac cccggtcagg gccacgaact
      301 caccgatggt gaacatggcc gcagaaacta cttgaccttc ccacgatggg aaggttgata
      361 ctcgctccat gcccgaacaa aacctgcgtc ggccgatccg ggtcgccgcc cagcttcatc
      421 cccagcacgg ggagtaccca cggctccatg ccaccgtcga ccaggcggag gagatgggat
      481 tcgacctgct ctacacctgg gatcacttct acccgctgta tggcgaacgc aacggtgccc
      541 acttcgaatg ctggacgatg ctcgccgcct gggccgagca gaccacgacc atcgagctcg
      601 gggcgctcgt cacctgcaac tcctaccgga acccggacct gttggccgac atggcccgca
      661 ccgtggatca catctccggg ggccgtctcg tgttcgggat cggcgccgga tggttccgaa
      721 gggacttcga ggagtacgga tacgagttcg gcaccgccgg ctcccggctc gagtcgctgg
      781 ccggggccat ccccaggatc aagacccgct gggagaagct gaaccctcct ccggtgcgac
      841 gcccaccgat cctgatcggg ggcggagggc tgcgaaagac gctgcgcatg gtcgccgagc
      901 acgccgactc ctggcacgcc ggctttcccg acgatcctgc cgagctcgag cccaaggtct
      961 ccgccctcct cggttggtgc gatcaattgg gacgcgaccc ggccgagatc gagtggggtc
     1021 tcggggtcga gcccgaggac ctggaccgat tcctcagtga cgacgtcgcc tcgtacgtgg
     1081 agatgggctt cacccagttc accctcgggt ttcagggccc ggactgggat ctcgggccgg
     1141 gaggcgactg gctggcctgg cgtgaccggg agaatctgcg atgaccgagg ccagggagcg
     1201 tggtcatcag atccgcttcg gggtggtcac cccgcagatg tggcactcgt ggggggagat
     1261 ggtcgagctg tggtcccgga tcgagggcgc gggatgggat gccgccttcg tcgtcgacca
     1321 tttcctctcc gactgggagg gggagatggg tgctcaactc gaggccttca cccttctcgc
     1381 cggtctggcc cgcgaagtcc cccggatcga catcggcacc ttcgtcgctg ggatcaccca
     1441 ccggcggccg gccgtgctcg ccaagcaggc agtgaccctg gaccacgtct ccgatggccg
     1501 attcatcttc ggggtgggtg ccgggtggaa cgagagagag cacgaggcat acggcatccc
     1561 cttccccgga gccgcggacc gggtgagcca ggtcggtgag gcactggagg cgatccggct
     1621 cctcgaggcc aataccacca ccgatttcac cggggagacg ctccgattga accaggctcc
     1681 gttcgagccg aagccggtca acgggcggct ccgggtcttg gtcggaagcc gtcgccccaa
     1741 aatgctcggt cacctcgccc gattcggcga cctgtgggat gcggcagggt cactcga
//
LOCUS       DATITW010000113         1810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_405784, whole genome shotgun sequence.
ACCESSION   DATITW010000113 DATITW010000000
VERSION     DATITW010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405784"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1810
                     /gene="uvrA"
                     /locus_tag="VLS86_01595"
     CDS             <1..>1810
                     /gene="uvrA"
                     /locus_tag="VLS86_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HSO49215.1"
                     /translation="GTERLVIHGAREHNLRNLTLELPRDALIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSSYARQFLGQMEKPDVDFIEGLSPAISIDQKSTSRNPRSTVGTI
                     TEVYDYLRVLFARVGRPHCPTCGRPIGRQTPEQIVDQVMELPEGTRFQVLAPVVRGRK
                     GEYGQLLQELARKGFPRARIDGEVRDLAEPIRLPKTYKHTIEVVVDRLVAKPDIRRRV
                     ADSIERALELAEGIASIAVITSEGHEEVQTFSQNLACTHCHLSFEELAPRNFSFNSPY
                     GACQTCDGLGTRLEVDPELIVPDPDLSVEDGALAPWASATLEYWYRVLQAVTQAHGFD
                     MTTPWKRLPKKAKEVLLYGSDESIYVQYKNRYGRQRAYWTTFEGAIPNIERRHTETDS
                     DAAREKLEQFMREIPCRACKGARLRPETLAVTIGGKNISELTALSIRDTLAFAQDMDL
                     SEREQMIAERLLKEIRSRLGFLVDVGLDYLTLGRAAGTLAGGEAQRIRLATQIGSGLV
                     GVLYILDEPSIGLHQRDNKRLIDTLIRLRDLGNTLIVVEHDEATILEADHIVDIGPAA
                     GEHGGEIVYSGDLKGLLECDDSLTGAYLSGRRSIPVP"
BASE COUNT          307 a          644 c          583 g          276 t
ORIGIN      
        1 gggcaccgaa cgcctcgtca tccatggcgc ccgggagcac aacctcagga acctcacgct
       61 cgagctcccc cgcgacgcgc tgatcgtctt caccggcctg tccgggtcgg ggaagtcgtc
      121 gttggcgttc gacaccatct acgcggaggg tcagcggcgg tacgtcgagt ccctgtcctc
      181 gtacgcgcgc cagttcctcg gccagatgga gaagcccgac gtcgacttca tcgaaggact
      241 ctcgccggcc atctccatcg accagaagtc cacctcccgg aaccctcggt cgaccgtcgg
      301 gaccatcacc gaggtctacg actacctgcg ggtgctcttc gcccgggtgg gtcgcccgca
      361 ttgccccacc tgcggccggc ccatcgggcg ccagaccccc gagcagatcg tcgaccaggt
      421 gatggagctc ccggaaggca cgcggttcca ggtgttggca ccggtcgtgc gaggccggaa
      481 gggggagtac gggcagctcc tgcaggagct cgcccgcaag gggttcccgc gggcccggat
      541 cgatggggag gtgcgcgacc tcgccgagcc catccgactc ccgaagacct acaagcacac
      601 catcgaggtc gtcgtggacc gactcgtggc caagcccgac atccgtcggc gggtcgctga
      661 ttccatcgag agggccctcg agctggccga aggcatcgcc tccatcgccg tcatcaccag
      721 cgaagggcac gaagaggtcc agaccttctc ccagaacctc gcgtgcacgc attgtcacct
      781 ctcgttcgag gagctggcgc cccgcaactt ctcgttcaac tccccgtacg gtgcctgcca
      841 gacgtgtgac gggctcggga cgcggctcga ggtggacccc gagctcatcg ttcccgaccc
      901 cgatctctcg gtcgaggacg gcgccttggc gccgtgggcc agcgccaccc tcgagtactg
      961 gtaccgggtc ctccaggccg tgacccaggc gcacgggttc gacatgacca ccccatggaa
     1021 gcggctgccg aagaaggcca aagaggtgct cctctacggc tccgacgagt cgatctacgt
     1081 ccagtacaag aaccggtacg gccgccaacg cgcgtactgg accacgttcg agggtgccat
     1141 ccccaacatc gagcggcgcc acaccgagac ggactccgac gcggccaggg agaagctcga
     1201 acagttcatg cgcgagatcc cgtgtcgcgc gtgcaagggc gcccggctgc gccccgagac
     1261 cctggccgtc acgatcggcg ggaagaacat ctcggagctc accgcgctct ccatccgcga
     1321 cacgctcgcg ttcgcccagg acatggatct ctccgagcgc gagcagatga tcgcggagcg
     1381 gctgctgaag gagatccggt cccgtctcgg gttcctggtc gacgtgggtc tcgactacct
     1441 gacgctgggc cgtgctgccg gcaccctcgc cgggggggag gcgcagcgca tccggttggc
     1501 cacccagatc gggagcgggc tcgtcggggt gctgtacatc ctcgacgagc cttcgatcgg
     1561 gctgcaccag cgcgacaaca agcggctcat cgacacgttg atccggctcc gcgacctcgg
     1621 caacacgttg atcgtggtcg agcacgacga agccaccatc ctggaggcgg accacatcgt
     1681 cgacatcggc cctgcggccg gcgagcacgg aggggagatc gtctactcgg gtgacctgaa
     1741 gggtctcctc gagtgcgacg actcgctgac gggcgcgtac ctgtcgggcc gccgcagcat
     1801 ccccgtcccc
//
LOCUS       DATITW010000114         1830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_446034, whole genome shotgun sequence.
ACCESSION   DATITW010000114 DATITW010000000
VERSION     DATITW010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446034"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VLS86_01600"
     CDS             complement(<1..418)
                     /locus_tag="VLS86_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49216.1"
                     /translation="MSRAEEIVRGVARWLNPLIGSPAGPLETSVFVDSFGPSLMPRTS
                     VHQGVATGLSVLAARAVTKIADVPFAALVPTDAGLAQRLAMRGVMGVTGKTLAAMPEE
                     EGETMWRSTARTTGRIVRAAAIGGAIYDLGIWLRDRN"
     gene            complement(415..1155)
                     /locus_tag="VLS86_01605"
     CDS             complement(415..1155)
                     /locus_tag="VLS86_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HSO49217.1"
                     /translation="MFNSIYPKPGVVDDLLDLVLEPSREVAGRPWVLLNMVASIDGAT
                     TVEGRSSGLGDDDDLAMFKALRAVPDVILVGAGTARAEDYGPVRLDGERVERRRALGM
                     AELPRLAVVTGRLDLDPKARLFSRPETPPLILTGPAADPGRRRALDAVAEVVVLEDLS
                     VEAVIGHLSPAKVVLCEGGPGLNAQLAAARLIDEVNLTLAPIMVGGVSKRIIEGVPTD
                     PVLEMVLDRLLSGERSLFFRYLKQDGQS"
     gene            complement(1175..>1830)
                     /locus_tag="VLS86_01610"
     CDS             complement(1175..>1830)
                     /locus_tag="VLS86_01610"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HSO49218.1"
                     /translation="NQLPMGTDIHWHGVKVPNSMDGVAPLTQPLVESGETFVYEFTAL
                     EPAIAMYHAHHHGQMQVPNGLFGTFIIGDTPVPRGQTISGVEIPEDLEIAHEIPMVLN
                     DAGVIGLTLNAKSFPATEPYVGKVGDWVVAHYFNEGLQIHPMHQHQFFQLVFAKDGVP
                     LDHPYWADTVAVAPGERYSVLFQLEDPGVWVWHCHILNHVESDTGMFGMVTAWIVEE"
BASE COUNT          345 a          614 c          559 g          312 t
ORIGIN      
        1 cattgcggtc gcgcagccag atgccgaggt catagatggc cccaccgatc gccgccgccc
       61 tgacgatccg gccggtggtg cgagcggtgg accgccacat cgtctccccc tcctcctccg
      121 gcattgcggc caatgtcttg ccggtcaccc ccatcactcc gcgcatcgcc agacgctggg
      181 ccaggccggc atcggtgggg accagtgccg caaatggcac gtcggcaatc ttggtgaccg
      241 ctctggcggc cagaacactg aggccggtcg ccactccctg gtgcaccgat gtccgtggca
      301 tcagcgacgg cccgaaggag tcgacgaaca ccgaggtctc gagcggtccg gccggtgacc
      361 cgatcagtgg gttgagccat ctcgcaaccc cgcggacgat ctcttcggcc cggctcatga
      421 ctgaccgtct tgcttcaggt aacggaagaa caacgagcgc tcgccgctga gaagacgatc
      481 gagaaccatc tcgagaaccg ggtctgtggg gacgccttcg ataattcgct tcgacacgcc
      541 gccgaccata atcggcgcca gagtgaggtt gacctcgtcg atcagccggg cagccgccaa
      601 ctgggcattg agaccaggcc cgccctcgca caggaccacc ttcgccgggc tcaggtgccc
      661 gatcaccgct tcgacactca gatcctcgag caccaccacc tcggcgaccg catcgagcgc
      721 tcggcgccga cccgggtccg cagccggccc ggtcaggatc aaggggggtg tctcgggtct
      781 ggaaaagagc ctggctttgg gatcgaggtc gagacggccc gtgaccacgg cgagtctggg
      841 caactcagcc atgcccaaag cccgtcgccg ctctacccgt tccccatcga gtcgtaccgg
      901 cccatagtcc tcggcacgag ccgtgcctgc ccccaccagg atcacatcgg gaacggcccg
      961 aagtgccttg aacatcgcca ggtcgtcgtc gtcacccaga cccgacgacc gcccctccac
     1021 agtggtggct ccgtcgatgg acgccaccat gttgagcagc acccatggac ggcctgcaac
     1081 ctcacggctt ggctccagca ccagatctag aaggtcgtca accacccctg gcttcggata
     1141 gatgctgttg aacatcgggg ccgaaaggta gcgatcactc ctcgacaatc cacgccgtga
     1201 ccatgccaaa catcccggtg tcgctctcca cgtggttgag gatgtggcag tgccacaccc
     1261 acacgccggg gtcctcgagc tggaacagca ccgagtaccg ctcgccagga gccactgcga
     1321 ccgtgtccgc ccagtaggga tggtcgagag ggaccccatc cttggcgaac acgagttgga
     1381 agaactgatg ctggtgcatg gggtggatct gcaggccttc gttgaagtaa tgggccacta
     1441 cccaatcccc gacctttccg acgtaaggct cggtggccgg gaacgacttg gcgttgaggg
     1501 tgagcccgat cacccccgca tcattgagaa ccatcgggat ctcatgtgcg atctccaagt
     1561 cttcggggat ctcgaccccg gaaatggtct gccctcgggg aacgggagtg tctccgatga
     1621 tgaaggtgcc gaagagaccg ttgggcacct gcatctgccc atgatgatga gcgtggtaca
     1681 tggcgatcgc aggctccagc gccgtgaatt catagacgaa ggtctcgccg gattccacga
     1741 ggggttgggt cagaggggcg accccgtcca tgctgttcgg caccttgacg ccatgccagt
     1801 ggatgtcggt gcccatcggc aactggttgt
//
LOCUS       DATITW010000115         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_646325, whole genome shotgun sequence.
ACCESSION   DATITW010000115 DATITW010000000
VERSION     DATITW010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646325"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VLS86_01615"
     CDS             complement(<1..197)
                     /locus_tag="VLS86_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49219.1"
                     /translation="MPARKRVLSVLAIEIRCAISTLGLIARRKLAQPKDLVQSQLEFA
                     DGSISRVYRETTKRDLETTPQV"
     gene            361..750
                     /locus_tag="VLS86_01620"
     CDS             361..750
                     /locus_tag="VLS86_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49220.1"
                     /translation="MIETKGRKPFRKEVISVTQVLERERTEITRTPVVRRATEWVVGV
                     AGAVAAAIGAYVYYAPTDWFLGDVSESWAFGLFTGAGVLLATAFGVFARKAYLEDRYM
                     SGRVMVATVLSVAAIAGAVTFALIWIL"
     gene            892..1356
                     /locus_tag="VLS86_01625"
     CDS             892..1356
                     /locus_tag="VLS86_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSO49221.1"
                     /translation="MEDLSLERCGQVLEDGWVGHLGVISDGEPYVAPISYVVVDGRVC
                     FKTGPGRRADAIQSSPRVCLETSHVDQSTGYWESVVVWGNAEEIREDVEAQTVISALV
                     SKYRPVLGSPLSPGPNDVGLGEPDILIALSIETITGRCSGSFFHISSRPGRL"
BASE COUNT          255 a          445 c          491 g          318 t
ORIGIN      
        1 acctgcggag tcgtctcgag gtcacgcttg gtggtttccc gatagaccct cgagatcgac
       61 ccgtcggcga actcgagttg tgattgcacc aggtccttgg gctgggccag cttgcgtcgg
      121 gcgatgagac ccagcgtcga aatggcgcat cgaatctcga tcgcgaggac tgacagcacc
      181 ctctttcggg cgggcatctg ttgactgctc atgatcgtca acggtgccgt ctggtctatt
      241 tggtagcaag ggtcgaaggt cacgacccgg gccgccgacg ctgccggcgc ctggtgccag
      301 agtcgcttgg acggtgccga ttgtgacctt cgcctctggg ctggatatcg ccgggtcggc
      361 atgatcgaga cgaaaggcag gaagcctttc agaaaggagg tgatctctgt tacgcaggta
      421 ctcgagagag agcggaccga gatcacccga acccctgtcg tgcggcgtgc caccgagtgg
      481 gtggtcggcg tggccggggc tgtggcagct gcgatcggag cgtacgtcta ttacgccccg
      541 accgactggt tcctcggaga cgtgtctgag agctgggctt tcggcctgtt caccggggcc
      601 ggagtcttgc tggccacggc attcggggtc ttcgcccgca aggcgtacct cgaagataga
      661 tacatgtcag ggcgtgtgat ggttgccact gtcttgtcgg ttgccgccat cgcgggcgcg
      721 gtgacattcg cgctcatctg gatcctctga cactgttgcg aaaggtccgg aggccaacgg
      781 cctccggacc ttcccgcgcc gttggtcata gcctgtttgg accaatggcc ctgggcctgg
      841 accggtgaat ctcttacgtt cgggacatga gtcgagtgat cgagtgacgc agtggaggac
      901 ttgagtctcg agcgttgcgg ccaggtcctg gaagacggtt gggtggggca cctcggcgtc
      961 atctccgatg gagagcccta cgttgccccg atctcttacg tcgtcgtgga tggacgagtc
     1021 tgcttcaaga caggcccagg gaggcgagcc gacgccatcc aatcatcacc cagggtctgt
     1081 ctcgagacca gccatgtcga ccagtcgacc gggtattggg agtcggtggt ggtctggggc
     1141 aatgccgagg agatcagaga ggatgtcgaa gcccagacgg tcatctccgc cctcgtctcc
     1201 aaataccggc cggtcttggg ctcaccgctt tcacctggtc cgaacgatgt gggtcttggc
     1261 gagcccgaca tcctcatcgc cctctccatc gaaaccatca ccggccggtg ttcgggcagc
     1321 ttctttcaca tttcgagtcg acccggacgc ctatgacacc ctcgatcaca gcttctccga
     1381 ccagtgccct tgcattcgcc tcgactcgtg taccgattgc ccacgtcgga gagaatgtcg
     1441 gggaggtgcg tgaccggttg gttggcggtg accggctcga aagcgcagcc cagattgccg
     1501 tcctacatg
//
LOCUS       DATITW010000116         1731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_45592, whole genome shotgun sequence.
ACCESSION   DATITW010000116 DATITW010000000
VERSION     DATITW010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45592"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VLS86_01630"
     CDS             complement(<1..597)
                     /locus_tag="VLS86_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49222.1"
                     /translation="MGRPDAPSADPLLIVDDRVSGATDEVLAAGVSLGMLRREAEALA
                     PFATILTRDLGEESRRFDPVVEAIEALIPRVEVVAPGLVFVPITGAVRFYGGETVLAE
                     LVTKEMDRLVGHVRGGQALVGVADGPFAARWAAATTSVGVPNVVTDSIGFISRLDLAA
                     LRESLSADELIDTFRWLGVSTLGDLARLPREALATRFGS"
     gene            complement(569..1228)
                     /locus_tag="VLS86_01635"
     CDS             complement(569..1228)
                     /locus_tag="VLS86_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49223.1"
                     /translation="MPDDGVSLGITVSPFVDLKQREEGYLHLQPGRVIELAGVPGTGL
                     TRLGLRLLAPPSLVGTVVALDSRGWISPEAAWETGVIPENLVIVRCDQAQIWPKVAAA
                     LLEGVRALYAEVPAGTKDQDLRRLAALARARKVSVAMRPLGDGLPPGVAHLRLRALGV
                     TWEGADQGHGQLKRRRLVLEASGKGASGVSRRFEVEDEGSDVVRLVSDVVVGETRRAV
                     G"
     gene            complement(1294..>1731)
                     /locus_tag="VLS86_01640"
     CDS             complement(1294..>1731)
                     /locus_tag="VLS86_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HSO49224.1"
                     /translation="DPRLFSGIGNAYSDEILWAARLSPVKRTSQLDGAEMELLHRAVV
                     DSLQDWTQRLREEVGDGWPTKVTAFRDDMAVHGKFGEPCPRCGSKVQRIVYADNETNY
                     CATCQTEGKLLADRSLSRLLKEDWPRTLEELEEVVPSSGDGSV"
BASE COUNT          320 a          609 c          529 g          273 t
ORIGIN      
        1 gctaccgaat cgagtggcaa gggcttcacg gggcagacgg gccagatcgc ccagggtgga
       61 tacgccgagc catcggaaag tatcgatcaa ctcgtcggca ctgagactct cgcggagggc
      121 agccaggtcg agacgagaga tgaatccgat gctgtcggtc accacgttgg gcacgccgac
      181 actggtggtg gccgccgccc agcgggcggc gaacggcccg tcggccaccc cgaccagggc
      241 ctgcccgcct cggacatggc ccaccagcct gtccatctct ttcgtcacca actcggccag
      301 caccgtctcc cccccgtaga agcggacagc cccggtgatg ggcacgaaca ccagaccggg
      361 ggccaccact tccacccgtg ggatcagggc ttcgatggcc tcgaccaccg gatcgaaacg
      421 tctggactct tcaccgaggt cacgggtcaa gatcgtggca aagggcgcca gggcctctgc
      481 ctcgcgacga agcatgccca ggctcacccc ggccgcgagc acctcgtcgg tggcaccgga
      541 gacccggtcg tcgacgatga ggagtgggtc agccgacggc gcgtcgggtc tccccaacga
      601 ccacatcgga aaccaaacgc acaacgtccg aaccttcatc ctccacctcg aatcgccggc
      661 tcacccccga agcgcctttt ccggacgcct ccagcaccag acgtcttctc ttcaattgcc
      721 catgaccctg atccgctccc tcccaggtga ccccgagcgc tctcagccgg agatgagcca
      781 ctcccggtgg cagaccgtct cccagggggc gcatggcgac cgagaccttc cttgccctgg
      841 ccagcgcggc cagacggcga agatcctgat ccttggtccc cgctggaacc tcggcgtaga
      901 gggcccggac cccctcgagc aaggcggcag cgaccttggg ccagatctgt gcctgatcgc
      961 aacggacgat gaccaggttc tccgggatca ctcccgtctc ccaggctgcc tccggtgaga
     1021 tccaccctct gctgtccagg gccaccaccg tgccgaccag tgagggtggg gccaggagtc
     1081 gcagaccgag acgggtcaac ccggtgccgg ggaccccggc caactcgatg acccggccag
     1141 gttggagatg gagataaccc tcttctcgct gcttcaggtc gacaaagggt gagactgtga
     1201 tgcccaagct gactccgtca tcgggcactc catgttatcg aacacatgtt cgatagggaa
     1261 atgggaaata ccgaacaggt caccctggcc ggactagacg gacccgtccc cagaagaggg
     1321 gacgacttcc tcgagctctt ccagcgtccg gggccagtcc tccttgagga gacgggagag
     1381 ggagcgatcg gcgagcagct ttccctcggt ctgacaggtg gcgcagtagt tggtctcgtt
     1441 gtctgcgtac acgatgcgct gcaccttcga gccacaccgg gggcagggct ccccgaactt
     1501 gccgtgcacc gccatgtcgt cgcggaacgc tgtcaccttc gtcggccacc cgtctcccac
     1561 ttcctcccgc agccgctggg tccagtcctg aagtgagtcg accaccgccc ggtgcaagag
     1621 ctccatctcg gccccatcga gctgcgacgt cctcttcacc ggtgagagac gggcggccca
     1681 caggatctca tcggagtagg cgttcccgat cccggagaag aggcgggggt c
//
LOCUS       DATITW010000117         1809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_125689, whole genome shotgun sequence.
ACCESSION   DATITW010000117 DATITW010000000
VERSION     DATITW010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125689"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VLS86_01645"
     CDS             complement(<1..908)
                     /locus_tag="VLS86_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HSO49225.1"
                     /translation="MATAITTGPGGLVVPDDPIIPFIEGDGIGPDIWAASVRVFDAAV
                     KKAYGDERAVAWQEVLAGEKAHNETGSWLPDGTVDAFSTHLVGIKGPLTTPIGGGFRS
                     LNVALRLTLDLYACVRPVRWFEGVPSPVKDPGQVDMVIFRENTEDVYAGLELEANTED
                     VAKLIDFVKSEYGWEVRPDSGVGLKPVSITGSKRLVRAALKYAVANRRKSVTLVHKGN
                     IMKFTEGAFRIWGYELVAEEFADVAVSWEDSGGEPGDKLLVQDVIADAMLQQVLTRPA
                     NSDVIATTNLTGDYISEALAAQVGGIG"
     gene            1073..>1809
                     /locus_tag="VLS86_01650"
     CDS             1073..>1809
                     /locus_tag="VLS86_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSO49226.1"
                     /translation="MRLFQDHASRARDLGISRQLIEDDLFSGDSIVLDGKRLANFGLC
                     SYLGLGDDERLKNGARRAIDKYGTSYSSSIAYTAIPLYRDLSERLATIFGASVVLAPT
                     TTLAHLAALPVLVRPGDQVLVDSVAHASLQTATQLLQANGIPVQKVPHNDLAATETAV
                     SEFDGAGRVWLLIDGVYSMHGDLAEAAELDTLLDRHPKLHVYCDDAHGFGWDGEHGRG
                     TYLARTSWHDRLVVAAGLSKSFGATGGV"
BASE COUNT          307 a          614 c          586 g          302 t
ORIGIN      
        1 ccgatccccc ccacctgggc ggcgagggcc tcggagatgt agtccccggt gaggttggtg
       61 gtggcgatca cgtcggagtt ggcgggccgg gtgagcactt gctgcagcat ggcgtctgcg
      121 atcacatcct ggaccagcag cttgtcaccg ggctcaccac ccgagtcctc ccaggacacg
      181 gccacatcgg cgaactcctc cgcaacgagt tcgtaacccc agatgcggaa ggcgccctcg
      241 gtgaacttca tgatgttgcc cttgtggacc agggtcaccg atttgcgccg gttggcgacg
      301 gcgtacttga gggcggcccg caccaggcgc ttcgacccgg tgatcgacac cggcttgagc
      361 ccgacaccgg aatcggggcg gacctcccac ccgtactcgc tcttgacgaa gtcgatcagc
      421 ttggcgacat cctcggtgtt ggcttccagc tcgagcccgg cgtagacgtc ctcggtgttc
      481 tcgcggaaga tgaccatgtc cacctgaccc gggtccttga ccggggaggg aacaccctcg
      541 aaccaacgga cggggcggac acaggcgtag agatccaagg tgagccggag cgccacgttg
      601 agggaccgga aacccccacc gatcggggtg gtcagcgggc ccttgatccc gaccaggtgg
      661 gtgctgaaag cgtcgacggt cccgtccggc agccacgacc cggtttcgtt gtgggccttc
      721 tccccggcga ggacctcttg ccaggcgacg gccctctcgt ctccataggc cttcttcacc
      781 gcggcgtcga agacccggac cgaggcggcc cagatgtcgg gcccgattcc gtcgccctcg
      841 atgaagggga tgatcgggtc gtcgggtacg accaatccgc ccggaccggt cgtgatagca
      901 gtggccattt gtcaggactc cctggataca gttaggaacc gtcggacact agcgaataag
      961 atcgggtgat ctcccccggg tcggcgcagg gaagcggacc ggggacgaac cgccaggcaa
     1021 cctcggagaa gtcgtggaga aagccctcag ccccacgcgg ggcgatcgga tgatgcgtct
     1081 gttccaagac catgcatccc gggcccgcga cctgggaatc tcccggcagc tcatcgaaga
     1141 cgacctgttc agcggggact cgatcgtcct cgacgggaag cggctggcca acttcgggct
     1201 ctgctcctac ctgggcctgg gcgacgacga gcgtctcaag aacggggcgc gtcgggccat
     1261 agacaagtac gggacctcct actcctcctc gatcgcctac accgcgatcc ccctctaccg
     1321 cgacctgagc gagcgtctgg cgaccatctt cggcgccagc gtggtcctgg ccccaaccac
     1381 cacactggcg catctcgccg cccttcccgt gctggtgcgc cccggtgacc aggtcctggt
     1441 cgactcggtg gcccatgcct cgctgcagac ggcaacccag ctcctccagg ccaacgggat
     1501 cccggtgcaa aaggtgcccc acaacgacct cgccgccacg gagacggcgg tctcggagtt
     1561 cgacggcgca ggacgggtct ggctgctgat cgacggggtg tactcgatgc acggcgacct
     1621 tgctgaagcg gccgagctcg acacgcttct cgaccgtcat cccaagctcc acgtctactg
     1681 cgacgatgcc cacgggtttg gctgggatgg cgagcacggg cgtggcacct atctcgcccg
     1741 aacctcatgg cacgatcggc tggtggtggc ggccgggctc tccaagtcgt ttggcgccac
     1801 cggaggtgt
//
LOCUS       DATITW010000118         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_45649, whole genome shotgun sequence.
ACCESSION   DATITW010000118 DATITW010000000
VERSION     DATITW010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45649"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VLS86_01655"
     CDS             complement(<1..588)
                     /locus_tag="VLS86_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49227.1"
                     /translation="MGQFSASLKVPGDTSSLPATVDIDDGRIRVASGDHEIGDWALVD
                     IHLTPGPEGVHVSAEGEELILQFSESAAFEEAAGLNDKKSVVSLPKPFKLPSLSKTSK
                     HASPGKSTTRPEGSAPTARVDQPAKLAPSAIEQNAVREAKPAKQPGTMDRALERAEKT
                     AGGRLPSWIFSRGGALVVLSALAVAIIFPALVSTLL"
     gene            complement(632..916)
                     /locus_tag="VLS86_01660"
     CDS             complement(632..916)
                     /locus_tag="VLS86_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HSO49228.1"
                     /translation="MSQSPYPEASQATTALVLGILGIVLCPILGPFAWSIGAKEMQAI
                     DAGRRPLENRGTANASKILGIVGTVLMGVYVIGAIIAIVFLSVATVTTSS"
     gene            complement(923..1210)
                     /locus_tag="VLS86_01665"
     CDS             complement(923..1210)
                     /locus_tag="VLS86_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HSO49229.1"
                     /translation="MSQSPYPEASQATTVLVLGILGVVLCQFLGPFAWSMGNKELQAI
                     DAGRRPPENRATANAGKILGIISTVLLAIGIVFIILLVIGTVTFSALDEIG"
     gene            1279..2193
                     /gene="coaA"
                     /locus_tag="VLS86_01670"
     CDS             1279..2193
                     /gene="coaA"
                     /locus_tag="VLS86_01670"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005832040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HSO49230.1"
                     /translation="MSQPRYLDFDRSEWSALRANTPLTLTVEDIDKIRGINVLLDMDM
                     VEDTYLPLSRLLNLHVQGSHQLATVTDTFLGSPASPVPFVIGVAGSVAVGKSTTSRVL
                     QELLARWPHHPRVELVTTDGFLFPNRILEEKGLMERKGFPESFDRRGLLDFVARAKSG
                     DDHLETPVYSHIAYDIVPDEVRLLVRPDILIVEGLNVLQAGDAGAFVSDYFDFSIFVD
                     ADIAYIRKWYIERFLTLRDTAFTQSDAYFRRYAGLSDEEAAKVALDIWERINEVNLVD
                     NILPTRERASLILEKAEDHSVQLVRLRR"
BASE COUNT          426 a          701 c          681 g          442 t
ORIGIN      
        1 cagcagcgtg gagaccagcg ccgggaagat gatggcgacc gccagggccg agaggaccac
       61 cagcgccccg cctctgctga agatccaact cgggaggcgc cccccggccg tcttctccgc
      121 ccgctccagg gcgcgatcca tcgtgcctgg ttgcttggcc ggtttcgctt ccctcaccgc
      181 gttctgttcg atcgcgctcg gagccagctt cgcgggctgg tcaactctgg ccgtgggagc
      241 ggaaccttcc gggcgggtcg tcgacttgcc cggggaggca tgcttggaag tcttcgacag
      301 gctgggcaac ttgaacggtt ttggcaagga aacgactgac ttcttgtcgt tgaggcccgc
      361 cgcctcttcg aaggccgcac tttcgctgaa ctggaggatc aactcctcgc cctcggccga
      421 gacgtgaacc ccttcggggc cgggggtgag gtggatgtcg accaacgccc agtcaccgat
      481 ttcatgatcg ccggatgcca ccctgatacg accgtcatcg atatcgacag tggccggtaa
      541 tgagctggtg tcaccgggga ctttcaggga agcgctgaat tgacccatgg ggtcatttat
      601 cggcagctaa tggcccgact tgtggaaaaa gtcaggaaga agtggtgacg gtggccacgg
      661 agagaaagac gatggcaatg atggcgccga tgacatagac acccattagc accgtgccga
      721 cgatcccgag gatcttggat gcgttggcgg tgccccgatt ctccagtggt cgtctcccgg
      781 catcgatggc ctgcatctcc ttcgcgccta tggaccatgc gaatgggccc agaatcgggc
      841 agaggacaat tcccaggatt cccaacacaa gggctgtggt tgcctgactc gcttccgggt
      901 aggggctctg gctcatcgcc ccttatccaa tctcgtctaa cgctgagaag gtgacagttc
      961 cgatcacgag gaggatgatg aacactatcc ctatggcgag gaggactgtg cttatgatcc
     1021 cgaggatctt gccagcgttt gcagtggcac ggttctcagg gggccgtctc ccggcatcga
     1081 tggcctgcaa ttccttgtta cccatcgacc acgcaaacgg gccgaggaac tggcaaagga
     1141 cgactccaag gattcccaac acaaggactg tggtcgcctg actcgcttcc gggtagggac
     1201 tctggctcat ctcctgagta tcgcacgtgg gttcgggtcg gtgcggcgca gaacaggcct
     1261 ggaagtagcc tgatcggagt gagccagccg cgatacctcg atttcgaccg ctccgagtgg
     1321 tccgcattgc gggccaacac ccccttgacc ctgaccgtgg aggacatcga caagatcagg
     1381 ggaatcaatg tgcttctgga catggacatg gtcgaggaca cctatctccc cctgtcccgt
     1441 ctgctcaacc tccacgtcca gggcagtcat caactggcga cagtgaccga caccttcctc
     1501 ggctccccgg cttcgccggt cccgtttgtg atcggggtcg caggctcggt cgcggtcggc
     1561 aagtcgacga catcacgcgt cctccaagag ctgttggcca ggtggccgca tcacccccgg
     1621 gtcgaactgg tgaccacgga tggctttctc ttccccaatc gaatcctcga agagaagggc
     1681 ctcatggagc gcaagggctt tcccgagagc ttcgacagac gtgggctcct cgacttcgtg
     1741 gccagagcca agtccggcga cgaccatctc gagactcctg tctattccca cattgcctac
     1801 gacatcgtcc ccgacgaggt gcgccttctg gtgcggccgg acatcctcat cgtggagggg
     1861 ctcaacgtgc tgcaggccgg cgatgccggg gcattcgtgt ccgactactt cgacttctcg
     1921 atcttcgtcg acgccgacat cgcttacatc cgcaagtggt acatcgagag gttcctgacg
     1981 ctgcgcgaca cggcgttcac ccaaagcgac gcctacttcc gccggtacgc cggcttgtcc
     2041 gacgaagagg cggcgaaggt ggcgctcgac atctgggagc ggatcaacga ggtcaacctc
     2101 gtcgacaaca tcctgcccac cagggagcga gcttccctga ttctggagaa ggcggaagat
     2161 cactcggttc agctggtccg gcttcgacgc tgatcgccgc cgaactcgga cggtaccctc
     2221 tcgaccaatg atcaagatgt tctccagagc
//
LOCUS       DATITW010000119         1566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_5789, whole genome shotgun sequence.
ACCESSION   DATITW010000119 DATITW010000000
VERSION     DATITW010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5789"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VLS86_01675"
     CDS             <1..493
                     /locus_tag="VLS86_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HSO49231.1"
                     /translation="VIGILLVGAAVFLLLYLFASDDDPEAGGSTTSSSSSSSSATSGS
                     STTTGPETSTTSSTLSTTTTTVPVRPPEEVRVVVLNSMGLSGAAGRMTQRLADAGYQT
                     QQADDFEPEQDPSRIWYREGFAAEANALLEFLPGAVIESIPDPVLEEGSDLVLVLGTG
                     FTE"
     gene            490..1353
                     /locus_tag="VLS86_01680"
     CDS             490..1353
                     /locus_tag="VLS86_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49232.1"
                     /translation="MTHLAAGSRRGIPKAWILPPLGVLLILIAVYLDLVSRSIMIDFV
                     AWWPVWALLVLLAILARGRRWGRVRLSGLVPVLFVLALAIFVTGNLRGLEAMPSASIQ
                     LIGPGAGAASTMALSAHVDGVLEVDSDQSSFLYVVEPVRRGGDVGPPAATEQIQGPNL
                     SVVLVPAADPGLYTFAGWLLDLAEGPTWNLSLGGEVVADLSRLRVSALQVNGTGKVAL
                     GAVTESVVVNVSGTFSIAVPSTAPVRVVGPAVVPDDWLETTEGFASPATGSGWVISVG
                     DGSSLTVIEAS"
     gene            1376..>1566
                     /locus_tag="VLS86_01685"
     CDS             1376..>1566
                     /locus_tag="VLS86_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49233.1"
                     /translation="MPSDRLRRIALVVWTAVGAVALGYVLLYVAGSVRVIWLPVAFAA
                     GMVFLLEPLVRAFERMRLPR"
BASE COUNT          223 a          498 c          532 g          313 t
ORIGIN      
        1 ggtgatcggg atcctgctgg taggcgccgc cgtgttcctt cttctctacc tgttcgcttc
       61 cgatgacgat ccggaggccg gcgggtcgac gacctcgtca tcttcgagca gcagcagcgc
      121 aaccagtggc tcgtcgacca ccacggggcc tgagacctcg accacgtcct cgacactcag
      181 caccacgacg acgaccgtcc ccgtgcgacc cccggaggag gtccgggttg tggtgctcaa
      241 ctcgatgggc ttgtccggcg cggcggggag aatgacccag cgactggcag atgccggcta
      301 tcagacccag caggctgacg atttcgagcc cgagcaggat ccgtcacgga tctggtatcg
      361 agaaggattc gccgccgagg cgaatgcgct cctggaattt cttcccggtg ccgtgatcga
      421 gtcgatcccg gaccccgtcc tcgaggaagg gtccgacctg gtcttggtgc tgggcaccgg
      481 cttcaccgaa tgacccatct cgccgccggc tctcgccgcg gcattcccaa ggcctggatc
      541 ctgcccccac tcggcgtgct cctgatcctg atcgcggtgt atctggatct ggtgagccgg
      601 tcgatcatga tcgacttcgt cgcctggtgg ccggtgtggg cgctgctggt gctgctcgcc
      661 atcctggccc gggggagacg atggggaaga gtgcgtctct ccgggctggt gccggtgctg
      721 ttcgtcctcg ctctggccat attcgtcacc ggaaatctca ggggtttgga ggcgatgccc
      781 tcggcctcga tccaactgat cggccccggc gccggagccg catcgaccat ggcgctttcg
      841 gctcacgtcg atggggtgct cgaagtggac agcgaccagt cgagttttct gtatgtggtg
      901 gagccggtgc gccgaggcgg cgatgtcggg cctccagcgg ccacggaaca gattcaaggc
      961 ccaaacctct cggtggtcct ggtgccggcc gcggaccccg gtctctacac cttcgccggc
     1021 tggctgctcg acctggcgga gggaccgaca tggaatctct ccctcggcgg ggaggtggta
     1081 gccgatctga gccggctgcg agtctctgcg ctgcaggtca atgggaccgg caaagtggcc
     1141 ctgggagccg tcaccgagag cgtggtggtc aacgtgtccg ggaccttttc gatcgcggtc
     1201 ccgtccacag ccccggtcag ggtcgtcggc cctgccgtgg taccggatga ttggctggag
     1261 accacagaag gcttcgccag ccctgccacc gggagtggtt gggtgatctc ggtgggagac
     1321 ggctcgtctc tgacagtcat cgaggcttcg tgaccgctcg ttacactttc tggctgtgcc
     1381 ctccgaccga ctgagacgca tcgccctggt cgtgtggacc gccgtcgggg cggtagcgct
     1441 tggttacgtg ctcctctacg tggcgggatc ggtgcgggtc atctggctgc cggtggcgtt
     1501 cgcagcaggg atggtgttcc tcctcgaacc gctggtcagg gcattcgaac ggatgcgtct
     1561 accgcg
//
LOCUS       DATITW010000120         3774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_646741, whole genome shotgun sequence.
ACCESSION   DATITW010000120 DATITW010000000
VERSION     DATITW010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646741"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="VLS86_01690"
     CDS             <1..1426
                     /locus_tag="VLS86_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSO49234.1"
                     /translation="IFEDLVADVPKEMGLKHEEIEFFVGKLWQVLTSCDARRQEIYET
                     IGWWEFVDAGNKSEGYQKFLAEGLTRSLVAANAQLANAHVEGDVGLALILDMLVPGPS
                     TDRVLNGPTNEVWLHPWLDYLTKSGVDYQFDRRIEKIVCKPEDGRPGGRIEAVHMSDS
                     AGNTYELDDADYYIAALPAEVMARLLDDGILDVDPSLEDIKELGTKYVEWMNGIQFYL
                     TEDMPLSNGHTIYLDSPWALTSISQQQFWPNHPLQSYGDGKVRGVISVDISNWHADGN
                     TVKKPAMYCSAEEIRTEVWEELKTSHSKDDKPLTDEMLCCWFLDPDIVRENWIKTLAA
                     PGPPTVSGLVPMDEDAEPLFVAYINTWRLRPQAFTAVSNLLLASDYVRTFTSVATMEA
                     ANEAARRAVNAIIQESGHRATLCRLWNVTEPRFAFLFRMIDQHRYDRGLPWKDPPLFA
                     KAATRAIARGGPVTTTARRMVTGS"
     gene            complement(1447..>3774)
                     /locus_tag="VLS86_01695"
     CDS             complement(1447..>3774)
                     /locus_tag="VLS86_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49235.1"
                     /translation="IDDGHWVDSASWAMAEKVVREVPSLLLVIATRPFGEGSGRSAPE
                     EYLRLLEDAGTEHVVVSELLPAEVMALVERCLGVSSVSGPVFEMIIEQADGHPYFSEE
                     IAFALRDAGLLIVENGESRLAPNVQDLRDVDFPSTVQDIIIGRFDRLSARQQSIMKVA
                     SVIGREFAVDTLVEVYPAELDAAQATDSLRSLVGLDLVQEVLLDRSRYRFRHAITRDI
                     AYGLLLSSQTSELHRAVAVSLETEHGDDLDAVATTLAHHWRNSLGAESESSDADKALD
                     YLGRAGRMSLRNFAHREAIGFLTDAIDLATQGKGALGQVTPSREVPSATLARMEQDLA
                     EAYLGMGRVDSSADHFERSLELHGYPVGHGKFGVGARLLAASALQLRHRLAKADVSAS
                     HAHTKEDLIEAATAYERLMKIYYYANDPGALITAAVSGLNLAEKAGTSPVLARIYANM
                     SIVAGIVPLHKLARLYARRSREVADEVDRPPEHAWVRLATSVYGVGVGDWETTEQDLR
                     DALDLYKRLGDTRQMEETLGTTGQMMTAQGRFAEVYQIGEQLKESGAGRDDAQGELWG
                     WLFQGTSLLRQGDYRTALSRFRNGELLLDAGVGAINVGWFYGLIAETYWRLGDRDRAR
                     AAAASASQALYPERPGVVFALDGFTGVTDIYLGEWATGRAREAAAAKSASELAVKKLR
                     AFSKVFPIGQPAAHRYAGAVREQSGNPEAARKEWEQGIEIAVDLQMPYEEGRLRLSLG
                     QLSGDKAHLETALGLFEATGAASFSEAARAALRDI"
BASE COUNT          670 a         1235 c         1225 g          644 t
ORIGIN      
        1 gatcttcgag gatctggttg cagacgtccc caaggagatg ggactgaagc acgaggagat
       61 cgagttcttc gtcggcaagc tgtggcaggt gctcaccagt tgtgacgccc ggcgccagga
      121 gatctacgag acgatcggat ggtgggaatt cgtcgacgcc ggcaacaagt cggagggcta
      181 tcagaagttc ctcgccgagg gtctgaccag gtcgctggtc gcggcaaacg cccaactcgc
      241 caacgctcat gtcgagggag acgtcgggct ggccctgatc ctggacatgc tcgtccccgg
      301 cccgagcacc gaccgtgtgc tcaacgggcc caccaacgag gtctggctcc acccctggct
      361 cgactacctc accaagagcg gtgtggacta ccagttcgat cggaggatcg agaagatcgt
      421 ttgcaagccc gaagatggcc gtcccggtgg gcggatcgag gcggttcaca tgtccgactc
      481 cgcggggaac acctacgagc tggacgacgc cgactactac atagctgcct tgcccgctga
      541 ggtgatggct cgcctgctcg acgacgggat actcgacgtc gatccctccc tcgaggacat
      601 caaggagctc ggcaccaaat acgtcgagtg gatgaatggg atccagttct atctcaccga
      661 ggacatgccg ttgtccaatg ggcacacgat ctacctcgac tcgccgtggg cccttacttc
      721 catctcccag cagcagttct ggcccaacca cccgctccag agctatggcg acggcaaagt
      781 gcgcggggtc atctcggtcg acatctccaa ttggcatgcc gacggcaaca cggtcaagaa
      841 gccggcgatg tactgctcag ccgaggagat acgcaccgaa gtctgggagg agctgaagac
      901 ttctcacagc aaggacgaca agccactgac cgacgagatg ctctgttgct ggtttctcga
      961 cccggacata gtgcgcgaga actggatcaa gacgctcgcc gcccccgggc cgcccacggt
     1021 cagcgggctg gtccccatgg atgaggatgc ggagccgctc ttcgtcgcct acatcaacac
     1081 ctggcgtctg cgaccgcaag ccttcaccgc cgtatccaac ctgctgctgg cttcggacta
     1141 tgtgaggacc ttcacctcgg tggccaccat ggaggcggcc aacgaggctg cccgccgggc
     1201 ggtcaacgcc atcatccaag agtcgggcca tcgggcgacc ctgtgccggc tgtggaatgt
     1261 gaccgaaccg cggttcgcct tcctcttcag gatgatcgat cagcatcggt acgaccgtgg
     1321 tctgccctgg aaagacccgc cactgttcgc caaggccgcc accagggcga tcgcacgggg
     1381 cggtccggtg acgacgactg cgcgaaggat ggtcaccggg tcctagcccg ggtcacccgg
     1441 gagagatcag atgtctctga gggctgcccg ggccgcttcc gagaaggaag cggcgccggt
     1501 ggcctcgaac aggcccaggg ccgtttccag atgggctttg tcacccgaga gctggcccag
     1561 gctcaaccgc aacctgcctt cctcgtaggg catctgcaga tcgactgcga tctcgatgcc
     1621 ctgctcccac tctttgcgcg cggcttccgg gttgcccgac tgctctctga ccgcgccggc
     1681 atagcgatgc gcggcgggct ggccgatggg gaagaccttg ctgaaggcgc gcaacttctt
     1741 gacggccaat tccgacgccg atttggccgc ggcggcctcc cgagcgcgcc cggtcgccca
     1801 ctcgccgagg tagatgtcgg tgacgccggt gaacccgtcg agagcgaaca ccaccccggg
     1861 acgttccggg tagagggctt gggacgccga agccgcggcg gcgcgtgctc ggtctcgatc
     1921 accgagtcgc cagtaggtct cggcgatgag accgtagaac cagcccacgt tgattgctcc
     1981 caccccggcg tcgagcagaa gctcaccatt gcggaatcgg gacagcgcgg ttcgatagtc
     2041 gccctgtcgg aggaggctgg tgccctggaa gagccaaccc cagagctcgc cctgcgcgtc
     2101 gtcgcggccc gcacccgact ccttcagctg ctcgccgatc tgatacacct cggcgaagcg
     2161 gccctgtgcc gtcatcatct ggccggtcgt gcccagggtc tcctccatct gccgggtgtc
     2221 accgagacgc ttgtagagat cgagggcgtc ccgtagatcc tgctcggtgg tctcccagtc
     2281 gccgacccca accccataga cactggtggc gagacggacc caggcgtgtt cgggggggcg
     2341 atcgacttcg tcggcgacct cccggctcct ccgtgcgtag agccgtgcca gcttatggag
     2401 agggacgatc ccggcgacga tgctcatgtt ggcgtagatc cgggcgagca ccggcgaggt
     2461 gcccgccttc tcggccaaat tcaaacccga gacggctgcg gtgatcagcg ccccgggatc
     2521 gttggcgtag tagtagatct tcatcagccg ttcgtaggcg gtcgcagcct cgatgaggtc
     2581 ctctttcgtg tgggcgtggg aggctgagac gtcggccttg gccagccgat gcctgagttg
     2641 cagtgccgag gccgcgagaa gcctcgctcc gacgccgaac ttgccatggc cgaccgggta
     2701 tccgtggagc tcgagagagc gctcgaaatg gtccgcgctc gaatcgaccc ggcccatgcc
     2761 gaggtaggcc tcggcgaggt cctgctccat gcgggccagg gtcgccgacg gcacctcacg
     2821 gctcggcgtg acctgcccca gtgcgccctt gccctgggtt gccagatcga tggcgtcggt
     2881 gaggaatccg atcgcctccc tgtgggcgaa gttgcggagc gacattcggc ccgcccggcc
     2941 caggtagtcg agggccttgt cggcgtcgct gctctccgac tcggcgccga ggctgttgcg
     3001 ccaatgatgg gccagcgtgg tggcaacggc gtcgaggtcg tcgccatgct cggtctccag
     3061 agatacggcg accgcgcggt gaagctcgct cgtctgcgac gagaggagca gcccgtaggc
     3121 gatgtcccgg gtgatggcat gacggaatcg gtaacgggac cgatccagca gcacttcttg
     3181 caccagatcc agcccgacca gggagcggag gctgtcggtg gcttgggcgg cgtccaactc
     3241 tgcggggtag acctcgacga gagtgtcgac cgcgaactcc cgtccgatca cgctcgccac
     3301 cttcatgatc gactgctgac gggccgacaa ccggtcgaag cggccgatga tgatgtcctg
     3361 gacggtgctg ggaaagtcca cgtcacgcag atcctggacg ttgggggcca gccggctctc
     3421 cccgttctcg acgatgagca gaccggcgtc tcggagagcg aaggcgatct cctcgctgaa
     3481 ataggggtga ccgtcggcct gctcgatgat catctcgaaa accggcccgg acacactcga
     3541 cacaccaagg caccgctcga ccagtgccat cacctcggcc gggagaagct cggagacgac
     3601 gacatgctcg gtgcctgcgt cttcgagaag acgcaggtac tcctcaggtg cggagcgccc
     3661 cgatccctcg ccgaaaggac gggtggcgat cacgagtagc agcgacggga cctcccgcac
     3721 cactttctcg gccatggccc acgacgccga gtccacccag tggccgtcgt cgat
//
LOCUS       DATITW010000121         1814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_126017, whole genome shotgun sequence.
ACCESSION   DATITW010000121 DATITW010000000
VERSION     DATITW010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126017"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..>1814)
                     /gene="cofH"
                     /locus_tag="VLS86_01700"
     CDS             complement(1..>1814)
                     /gene="cofH"
                     /locus_tag="VLS86_01700"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HSO49236.1"
                     /translation="PTATSRRREGTTTVEMRSPSVAEIGAGHKAGLLVVAETVADVSP
                     AAWVVGPVEALELGERGVPGWRITVLGEGPKESILDAVARTRARYVRCTRSETTDIAA
                     ITSLPGIDCSVSVYADDVPQALRAIEQGATDLLLRDWDVERIGELRLALGDHLLVERS
                     VYPPGTEIDEAREVLPAEAFTAWVGQTDGSGAARPRYEWAPGRDMPPPVPADRLSAEW
                     LDVSWVGGLASTFPDSAGDLAPVLHRSLEGIAPTTSEVEALFRARGDQVDAIAHVADI
                     LRERAVGDTVTYVVNRNINYTNLCYFRCGFCAFSKGPRSLNLRGEPYLMGLDEIVDAA
                     AEAWDRGATEVTLQGGIHPDFTGNFYVDVVNAIKERIPGMHIHGFTPLEVWQGAETLG
                     IPVRRFLEELRQAGLGTLPGTAAEILDDEVRIHLCPDKIRTSQGAEVMITAHELGLRA
                     TSTIMFGHIDHPRAWANHLEVIRHIQRRTGGFTEMVPLPFVHMGSPIFLRGRSRPGPT
                     WDEVVLIHAVSRIALDGLVPNIQASWVKLGLDGGRRLLDAGCNDLGGTLMGEIITRSA
                     GAEHGQEVSPQQFREVIDAAGRTPALRSTTYEILDRV"
BASE COUNT          293 a          627 c          603 g          291 t
ORIGIN      
        1 ctagacccgg tccaggatct cgtaggtagt cgagcgcagg gccggggtgc gccccgcggc
       61 gtcgatcacc tcgcggaact gctgaggtga cacctcctgg ccgtgctcgg cacccgccga
      121 gcgggtgatt atctccccca tcagggtccc ccccaggtcg ttgcacccgg catcgagcag
      181 gcggcgaccc ccatcgagac cgagcttgac ccacgacgcc tggatgttgg gcaccagacc
      241 gtcgagggcg atccgggaca cggcgtggat cagcaccacc tcgtcccagg tgggaccggg
      301 ccgtgaccgc ccgcgaagga agatgggcga ccccatgtgg acgaagggaa gcggcaccat
      361 ctcggtgaag cccccggtcc tgcgctggat gtgacgaatg acctcgagat ggttggccca
      421 ggcccggggg tggtcgatgt gtccgaacat gatcgtcgag gtggcccgca gccccagctc
      481 gtgggccgtg atcatcacct cggccccctg cgaagtcctg atcttgtccg ggcagagatg
      541 gatgcgaacc tcgtcatcga ggatctctgc cgcggttccg ggcagggtcc cgagaccggc
      601 ctgtctcagc tcctccagga agcgacggac cgggatcccg agtgtctccg cgccctgcca
      661 cacctcgagc ggggtgaatc cgtggatgtg catccccggt atgcgctctt tgatcgcgtt
      721 caccacgtcg acgtagaagt tgcccgtgaa atcggggtgt atcccgccct ggagggtgac
      781 ctcggtggcc ccccgatccc acgcctcggc ggcggcgtcg acgatttcgt cgagacccat
      841 caagtagggc tcgccacgca ggttgagact gcgcggtccc ttggagaaag cgcagaaacc
      901 gcatcggaaa tagcagagat tggtgtagtt gatgttccgg ttgaccacat aggtcaccgt
      961 gtcgcccacg gcccgctccc gcaggatgtc ggcgacatgg gcgatcgcgt cgacctggtc
     1021 accccgggcc cggaacagcg cttcgacctc gctggtggtc ggggcgatcc cttcgagtga
     1081 gcggtggaga accggcgcga ggtcgccggc cgaatccggg aatgtcgatg cgagcccgcc
     1141 cacccatgac acgtcgagcc actcggccga cagccggtcc gcggggaccg ggggcggcat
     1201 gtctctcccc ggcgcccact cgtagcgggg gcgggcggcg cctgagccgt cggtctgacc
     1261 cacccaggcg gtgaatgcct ccgccggcaa cacctcccga gcttcgtcga tctcggtccc
     1321 cggcggatac accgatcgtt cgaccagcag gtgatcgccc agagcaagtc gcaactcgcc
     1381 gattcgctcg acatcccaat ctcgaagcag gagatcggtg gctccctgtt cgatggccct
     1441 gagcgcctgg gggacatcgt cggcatagac agagacagag cagtcgatcc cggggagcga
     1501 cgtgatcgcc gcgatgtcgg tcgtctcgga tctggtgcag cggacatatc tggcccgggt
     1561 gcgggcgacg gcgtccagaa tcgactcctt cggcccttcc ccgagcacgg tgatcctcca
     1621 accggggacc ccacgctcac ccagctccag ggcctcgacc gggccgacca cccaggccgc
     1681 cggcgacacg tcggcgacgg tctcggccac caccagcagt cccgccttgt gcccggcccc
     1741 gatctcggcc acggatggag atctcatctc gaccgtggtg gttccctccc ggcggcggct
     1801 ggtcgccgtg ggct
//
LOCUS       DATITW010000122         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_766984, whole genome shotgun sequence.
ACCESSION   DATITW010000122 DATITW010000000
VERSION     DATITW010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766984"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VLS86_01705"
     CDS             complement(<1..843)
                     /locus_tag="VLS86_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HSO49237.1"
                     /translation="MSPTVRPVVLSGGAGTRLWPLSTADRPKQFLELFGESLFEATLN
                     RLQGMVGVGPVTVVTGIDHLEPVERAAEIVAVDLASIVIEPVGRNTAAAVVAAALVSD
                     PEDVLVVLPSDHTIADVEAFHTVVETAISLATDGDLVTFGALALRPETGYGYIEMGEP
                     IEGGFRVARFKEKPDASEATRMVKSGTHLWNSGMFVFAAGHLLDEASAHSPRLVQDVD
                     QAMPSERTGRVFLDDSFATVESISIDHAVMEKTDKAVVIPMDVGWSDIGSWQSVWELT
                     GRDDS"
     gene            complement(865..2154)
                     /locus_tag="VLS86_01710"
     CDS             complement(865..2154)
                     /locus_tag="VLS86_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49238.1"
                     /translation="MKFTGQVRVPEIDHPGVPATILVEDGQTEIFLEGESLGRWSLYD
                     VRADRLVSSAFSLSLHGEEITFIADEPVDFAYKGVDHMAEVWARYKSMAMPRRALAVG
                     KSRRGTLPSRIGELRTAMLQNLEAAGRGMTVLPPIETVRGPLAKYTAQPSTREARPAT
                     PPVTEKVVAPPPSEPLFRPSPVEEAPSTPEVAATAAPSPPPRLRPGPGLVGRTARPAT
                     EPLPADSAEAAESEEALESVFASPEVTTPVLRSPWDEPALPDDHEEPVPVEVFAPESV
                     EDQAPQDPVVQIEPDPVLEEGAELVEAPLAEEVIPPAAPAEMPELVKELLSTSTDEAG
                     EEGARDLVVDLGPYEARGRRDTGPVAPPTSSPSPKEPAMAAAVPPDKSGLMGAVRSAF
                     ARNKVAHEHQFVEAPGGIGIVRQICAECGYISIGLSD"
BASE COUNT          402 a          805 c          767 g          396 t
ORIGIN      
        1 cgaatcgtcg cgcccggtca gctcccaaac cgactgccac gacccgatgt cgctccatcc
       61 gacatccatc ggtatgacga cggccttgtc ggtcttctcc atcaccgcgt ggtcgatcga
      121 gatcgattcg acggtggcga acgagtcgtc gaggaatact ctccccgtgc gctcggaagg
      181 catcgcctgg tcgacatctt gcaccaggcg cgggctgtgc gcactggcct cgtcgagcag
      241 atgacctgcg gcgaagacga acatgcccga gttccagagg tgggtcccgc tcttcaccat
      301 ccgcgtcgct tcggaagcat cgggcttctc cttgaatcgg gcgacgcgaa acccgccttc
      361 gatcggctcg cccatctcga tgtacccgta gccggtctcg ggccgaagcg cgagtgcccc
      421 gaaggtcacc agatcaccat cggtggcgag cgagatcgca gtctccacca cggtgtgaaa
      481 agcctcgaca tcggcgatgg tgtggtcgga ggggagaacg acgaggacat cctcgggatc
      541 tgagacgagg gccgcggcca ccaccgcggc cgcggtgttc ctccccaccg gttcgatgac
      601 tatcgaggcc agatccactg cgacgatctc cgcagcacgc tcgacgggct cgaggtggtc
      661 gatgccggtc accaccgtca ctggcccaac cccgaccatt ccctgtagcc ggttcagggt
      721 cgcctcgaac aacgattcgc caaacagctc gaggaactgc tttgggcggt cagccgtcga
      781 caacggccaa agccgggtgc cggcacctcc ggagaggacg accggtcgga cggttgggct
      841 cacccgggga gggtaccggc gctatcagtc agagaggccg atcgagatgt agccgcactc
      901 ggcgcagatc tggcgaacga tccctatccc gcccggagcc tctacgaact gatgctcatg
      961 agccaccttg ttcctggcga acgccgagcg caccgctccc atcaagccac tcttgtcggg
     1021 agggactgca gccgccattg caggttcctt gggggaaggg ctcgaggtcg gcggtgcgac
     1081 gggcccggta tcgcgacggc ccctggcttc gtatggaccc agatcgacca ccaggtcgcg
     1141 tgctccctcc tcgccggcct cgtcggtgga ggtgctgagc agctccttga ccaattccgg
     1201 catctcggcc ggggcggcgg gtggaatgac ctcttcggcg agtggcgcct ccacgagctc
     1261 cgccccctcc tcgagcaccg gatcgggctc gatttgcaca accggatcct ggggcgcctg
     1321 gtcctcgacc gattccgggg cgaacacctc gaccgggacg ggctcctcgt gatcgtcggg
     1381 aagtgccggc tcgtcccatg gggatctgag gaccggcgtg gtgacctccg gtgaggcaaa
     1441 gaccgactcc agcgcctcct ccgattctgc cgcctccgcc gagtccgcgg gcagtggctc
     1501 cgtggccggc cgggccgttc ggcccacgag cccagggccg ggtcggagac ggggcggcgg
     1561 tgagggagcg gcagtcgccg ccacctcggg tgtcgatggc gcctcttcga ccggtgatgg
     1621 gcgaaacaac ggctcgctcg ggggcggggc cacgactttc tcggtgaccg gtggggtggc
     1681 gggcctcgct tcacgggtcg aaggctgcgc ggtgtacttc gcgagcggtc ctcggacggt
     1741 ttcgatcggg ggcagaaccg tcatgccacg tcccgccgct tccaggttct gcaacatcgc
     1801 ggtgcgcagc tcgccaattc gggaaggcag cgttccccgg cgcgacttgc cgacggccag
     1861 ggcgcggcgg ggcatcgcca tcgacttgta gcgggcccac acctctgcca tgtgatcgac
     1921 gcccttgtat gcgaaatcga ccggttcgtc ggcgatgaag gtgatctcct cgccatgcag
     1981 cgacagcgag aaagcactgg agacgagacg gtcggcccgc acgtcgtaca acgaccagcg
     2041 tcccagagac tccccctcga ggaagatctc ggtctggccg tcttcaacca ggatcgtggc
     2101 cgggacccct ggatggtcga tctccggaac ccttacctga ccggtgaact tcaccgtgac
     2161 accgtcttct ctgcatcact acccgcgcca gtgctgacca tcaagatccc atttacccgc
     2221 caaaagtcaa gcaattcaag cgtaggcgtc gagcggcgga caggcgcaaa ccaggtttcg
     2281 gtcccccccg ggcccgtcga cccgacccac gggaggccag tacttgtcga cccgaagcga
     2341 gggcaccggg taagccgcag tctcacgtgt
//
LOCUS       DATITW010000123         1565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_126078, whole genome shotgun sequence.
ACCESSION   DATITW010000123 DATITW010000000
VERSION     DATITW010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126078"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VLS86_01715"
     CDS             complement(<1..493)
                     /locus_tag="VLS86_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49239.1"
                     /translation="MSRRTQFRLLVAFTIVWAVWAGWSLLGRATPYTLRVLDDSGAPV
                     ASAFIDVDGRQVGTSAEDGLVEMEWNSSKSVVEVSAPGHVPLTLTVAERPDGVVDAVI
                     KARILRGKVIDGAGVPVESAHVVAGPARGLSDAEGEFTIRGAEPGTIQVSRPAWIETS
                     FVWD"
     gene            597..1547
                     /locus_tag="VLS86_01720"
     CDS             597..1547
                     /locus_tag="VLS86_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSO49240.1"
                     /translation="MGKPIRASDARPERGSGPGDTVGLYLDEVSAHLLLTAEDEVHLA
                     RAMERGKKAKSLLESGDFQAAERPQLMRLIAEGEEAKMTFIRSNLRLVISIAKRYTGR
                     GLDLLDLIQEGNLGLIRAVEKFDWRKGFKFSTYATWWIRQAITRGLGNNGRTIRLPVH
                     MVDVVRTVQDTKQSLHDKYHRAPTLEEISEASGLDTERVLAALEAPAETVSLDRPVGE
                     DGDASLQDFVEDTGAANPEDRAASAWRRERLIKALQILDLEEQQVLWLRFGLDGDEPW
                     TLSDVGKVVYCTRERVRQIEARGLAKLRHPSCDIDLQALL"
BASE COUNT          317 a          501 c          482 g          265 t
ORIGIN      
        1 catcccaaac gaagctggtc tcgatccacg ccgggcggga cacctggatc gtccccggct
       61 cggcacctcg gatcgtaaac tcgccttcag cgtcgctcaa acctctcgcc gggcctgcca
      121 ccacgtgggc agactccacc gggaccccgg caccatcaat cactttccct ctgaggattc
      181 tcgctttgat gacagcgtcg acaacgccgt ctgggcgctc ggccacggtg agggtgagcg
      241 ggacgtggcc aggagcgctc acctcgacga cactcttcga agagttccac tccatctcga
      301 ccaagccgtc ctcggcgctg gtgccgacct gacggccgtc gacgtcgatg aacgccgatg
      361 ccaccggggc cccggagtcg tcgagcacac gcaatgtgta gggagtagcg cgacctagca
      421 gtgaccaacc agcccacact gcccagacga tggtgaacgc caccagcaaa cggaactggg
      481 ttcggcgcga cactgagaaa tggaggttac ccagggcgaa tctgtaagcc gcgattttgg
      541 tttgccagat tcaaccagga gggttagttt tcaacagggt tgttgaggag gtcagattgg
      601 ggaagcccat cagggccagc gatgcgagac cggaacgggg ctcaggtccc ggtgacaccg
      661 tcggtctata cctggacgag gtgtccgccc atcttcttct gacggccgaa gacgaggtcc
      721 atctggcccg ggccatggag cggggcaaga aggcgaaatc gctgctcgaa tctggcgatt
      781 tccaggccgc ggagcgcccc cagttgatgc gtctcatcgc agagggcgaa gaggccaaga
      841 tgaccttcat ccgctccaac ctccggctcg tgatctcgat cgcgaagcgt tacaccggac
      901 gtgggctcga ccttctcgat cttatccagg agggaaatct cggactgatc cgggctgtgg
      961 aaaagttcga ctggcgcaaa ggcttcaaat tctccaccta cgccacgtgg tggatacgtc
     1021 aggccatcac cagaggcctt ggcaacaacg gccggactat ccggctcccg gtgcacatgg
     1081 tcgacgtggt ccgcaccgtt caggacacca agcaaagcct ccacgacaag taccaccggg
     1141 cacccactct cgaggagatc tccgaggctt ccgggctcga caccgagcgg gtcctcgccg
     1201 ctctggaggc tccggccgag acagtttcgc tcgaccgccc cgtcggcgaa gacggggatg
     1261 cgtcactcca ggattttgtc gaagacaccg gagcggccaa ccccgaggat cgggcagcct
     1321 cggcctggcg acgggagcgt ctgatcaagg ccctgcaaat cctcgacctg gaagaacaac
     1381 aggtgctctg gctgcgtttc gggctcgacg gtgacgagcc gtggaccttg tccgacgtgg
     1441 gcaaggtggt ctactgcacc cgggagcgag tacgtcagat cgaagcccgg ggcctggcga
     1501 aactgcgcca ccccagttgc gatatcgacc tccaggcgct gctgtagaag gcctcaggcc
     1561 ataag
//
LOCUS       DATITW010000124         3118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_366351, whole genome shotgun sequence.
ACCESSION   DATITW010000124 DATITW010000000
VERSION     DATITW010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366351"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VLS86_01725"
     CDS             complement(<1..435)
                     /locus_tag="VLS86_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49241.1"
                     /translation="MIAALFFTWFFAASLWTINALRRPVPPGRGFPPLWLPGMIVSEL
                     APWLFVLRALIAAGFVALGALDLWIGRAGLALFLLSEVGLLPLVVRSWRSSKEAGGRP
                     LPWALFLVRARPPKTIVTTTYVHYWDDLTLDVYRRPDASHAPC"
     gene            complement(432..803)
                     /locus_tag="VLS86_01730"
     CDS             complement(432..803)
                     /locus_tag="VLS86_01730"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HSO49242.1"
                     /translation="MDGGGLYRMAVRLMPETAEALATSSGIAIEDIDVLVAHQANDRI
                     LEGVRHRLGMRPEQVPSNIANYGNTTAATLPILFHELRGAGRVSPGHWSALPPSGRAP
                     TTAQPCTGCRPIRSPESLRPP"
     gene            complement(912..1187)
                     /locus_tag="VLS86_01735"
                     /pseudo
     CDS             complement(912..1187)
                     /locus_tag="VLS86_01735"
                     /inference="COORDINATES: protein motif:HMM:NF020134.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1203..1502)
                     /locus_tag="VLS86_01740"
     CDS             complement(1203..1502)
                     /locus_tag="VLS86_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49243.1"
                     /translation="MCVPDNVVTNDDLARIMETSDDWIASRTGVRQRRFADPGVGASD
                     LGAEAAMLAIADAGVAPSDVDIRVTATMTPDHMAPGIAALVQAKAGLGHIAAFDL"
     gene            1592..2578
                     /locus_tag="VLS86_01745"
     CDS             1592..2578
                     /locus_tag="VLS86_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HSO49244.1"
                     /translation="MIDNTISRRLITIPVVVVMFLLVSVGLPFLIATGLLVDLARAGL
                     SGRPAMASRLIVFLWLYLLGEMLALLALAFAGLHSHDRAVDLTYRFQAVWAAWNLNAI
                     EQVFGLTFVAEGLDQVPPPPILLLARHASLIDTMLPARYVARDHGIMLRYVLKKQLLI
                     DPALDIGGNRLPNHFVDRGSGDTESEIASIRALGAGMSDAEGILIYPEGTRYSEEKRV
                     RYVGSLLRKGGPLAEIAAGFERVLPPRSGGTLALLDATTADVVVLAHRGLEGLATVKD
                     IWSGELIGSRIDVKLWRVSRAGIPRDRSARVEWLYRLWGDVDHWVSERRRVG"
     gene            2575..>3118
                     /locus_tag="VLS86_01750"
     CDS             2575..>3118
                     /locus_tag="VLS86_01750"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HSO49245.1"
                     /translation="MTSTGDLLVVAAVTVLVLMILAWLLSLLVKDASIVDMIWGLGFV
                     IVAWATYLQAAERGGRGLLLTVLVTVWGLRLSGYLIWRNLGKPEDFRYREMRARAPRW
                     FWLVSLFQVFLLQGALMWVISAPVVVTLLGGGDLYWLDYLGIALWAVGLFFESIGDIQ
                     LARFKSQPGSTGKVMDRGLWR"
BASE COUNT          494 a         1019 c         1039 g          566 t
ORIGIN      
        1 acacggtgcg tggctggcgt cgggccggcg gtagacgtcg agggtgagat catcccagta
       61 gtggacgtag gtcgtggtca cgattgtctt cggtggtcgt gccctgacca ggaagagggc
      121 ccacggcagg ggacgaccac ccgcttcctt cgacgatcgc cacgatctga cgacgagggg
      181 cagcagtcca acctcggaga gcaggaacag ggccagcccg gcccgcccga tccacagatc
      241 cagggcccca agggccacga acccggctgc gatcagagcg cggaggacga agagccaggg
      301 cgccagctcg gagacgatca tgcccggcag ccacagtggg ggaaagcccc gccctggggg
      361 caccggccgg cgaagagcgt tgatggtcca caggctggcg gcgaagaacc aggtgaagaa
      421 cagcgccgcg atcatggcgg ccggaggctc tctgggctac ggatcggccg gcacccggta
      481 cagggctgcg cagtagtggg cgcccgcccc gaaggcggca aagccgacca gtgcccgggg
      541 ctgacccgac cggctccacg cagctcgtgg aacaggatcg gcagcgtcgc cgcggtggtg
      601 ttgccgtagt tggcgatgtt cgagggcacc tgctcgggtc tcatgccgag ccgatgtcgc
      661 actccttcca gaatccggtc gttggcctgg tgggcaacca gcacgtcgat atcctcgatc
      721 gcgatgcctg agctcgtggc cagcgcctcg gccgtctcag gcatcaatct gaccgccatc
      781 cggtagagac caccgccatc catcaccggc agatgcgcat cgacatcgat ctggcggcgg
      841 ctgacatacg ggcgctcctt gaaccccacg ccgggaaccc agatcagctc gaagccggcg
      901 ccgtcggtgt gaagcgacgt ggcgagcaga ccttcctcgg gctcgtcccc ccggcgcagc
      961 accatggccc cggctccatc cccgaacagg acgctccacg cccgatacct ggtgttgcgt
     1021 gcgtactcgg cctcgcgcaa cgtccccttg ccccggccca ggacctcgtc ccaggagtcg
     1081 gcgccccacg ggaggaagcc ggcgtggacc tccgctccga ccacgaccgc gatctcgcca
     1141 cgactcgagg agatcatcgc atccgccagg tcgagaccgt agagaaaccc gctgcattgc
     1201 tgtcacaagt cgaaggctgc gatgtgcccc aaccccgctt ttgcctggac caacgcagcg
     1261 attccaggtg ccatgtggtc gggggtcatg gtggcggtca cccggatgtc cacgtcgctg
     1321 ggggcgaccc cggcgtccgc gatcgccagc atcgcagctt ctgccccgag gtccgaggca
     1381 ccgactcccg ggtcggcgaa tcgacgctgt ctgaccccgg tgcggctggc gatccagtcg
     1441 tctgaagtct ccatgatccg ggccaggtca tcgttggtga ccacgttgtc gggtacgcac
     1501 atcccggtcc cgacgatcac atttcccaag tggggcctat ttctttcatc cgccggatat
     1561 tcggcgccga acttagtgtt ggattgccgc agtgatcgac aacaccatct ccagacgcct
     1621 gatcacgatc ccggtggtcg tggtcatgtt cctcctggtc agcgtcggtc ttccatttct
     1681 gatcgcgacc ggcctgctgg tcgatctcgc ccgggcaggg ctctcgggaa gaccggccat
     1741 ggcatcgcgg ctcatcgtgt ttctctggct gtatctgctc ggtgagatgt tggcgctgct
     1801 tgccctcgcc tttgccgggc tgcactccca tgaccgcgcg gttgacctca cctaccgatt
     1861 ccaagcggtt tgggccgcct ggaacctgaa cgcaatcgaa caagtcttcg ggctgacctt
     1921 cgtggcggag gggctggacc aggtcccgcc gcccccgatc ctcctcctcg ccagacatgc
     1981 gtcgctgatc gacaccatgc tccccgcccg ctatgtggcc cgtgaccacg gaatcatgct
     2041 ccgctatgtc ctcaagaagc agttgctcat cgacccggcc ctcgacatcg ggggcaaccg
     2101 gcttcccaat catttcgtcg accgtggttc gggtgacacc gaatccgaga tagcgtcgat
     2161 ccgtgccctc ggcgccggga tgagcgatgc cgagggcatt ctgatctatc cggaggggac
     2221 gaggtactcc gaggagaagc gggtgaggta tgtgggctct ctgctgcgaa agggaggtcc
     2281 cctggcggag atcgcagccg ggttcgagag agtcctccct ccccgctccg gggggacgct
     2341 ggccctcctc gacgcaacga cggccgacgt cgtggtgctt gcccatcgcg gtctcgaagg
     2401 gttggccacg gtcaaggaca tctggtcagg cgagctcatc ggctccagaa tcgacgtcaa
     2461 gctgtggagg gtgtcccggg ccggcatccc ccgcgatcgc tcagctcggg tggagtggct
     2521 ctaccgactc tggggcgatg tggaccactg ggtgtcggag agacggcgcg tcggatgaca
     2581 tccaccggcg acctgctcgt ggttgcggca gtgaccgtgc tcgtcttgat gatcctggcc
     2641 tggctgctca gcctcctcgt gaaggacgcg tcgatcgtgg acatgatctg gggtctcggg
     2701 ttcgtgatcg ttgcttgggc gacctatctc caggcggcgg agcggggagg gcgcgggctg
     2761 ttgttgaccg ttctggtcac ggtgtgggga ctgcgcctga gtggctatct gatctggcgc
     2821 aacctgggca agcccgagga ctttcgctac cgggagatgc gggcccgcgc cccgaggtgg
     2881 ttctggctgg tcagcctgtt ccaggtgttt ctgctccagg gcgccctgat gtgggtgata
     2941 tcggcgcccg tggtcgtgac tctgctgggg ggcggcgatc tctactggct cgactatctc
     3001 gggatcgccc tatgggcggt cggcctgttc ttcgagagca tcggggacat ccagctcgcc
     3061 cggttcaagt cccagccggg ctccacgggc aaggtgatgg accgtgggct gtggcgct
//
LOCUS       DATITW010000125         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_6030, whole genome shotgun sequence.
ACCESSION   DATITW010000125 DATITW010000000
VERSION     DATITW010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6030"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..493)
                     /locus_tag="VLS86_01755"
     CDS             complement(125..493)
                     /locus_tag="VLS86_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HSO49246.1"
                     /translation="MDLFWSMLWFFMFFLWIWLLISLFADIFRSDDLSGWGKAGWVIF
                     MVLLPWLGALIYLIARGKGMQERAVADMAAREQATRQYIQEVATPTSTADELSKLAAL
                     RDQGVLTSAEFESQKAALLG"
     gene            597..1001
                     /locus_tag="VLS86_01760"
     CDS             597..1001
                     /locus_tag="VLS86_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49247.1"
                     /translation="MAPIPIHPGKGMRKSPDSAMFATKESPDEETPLTSAQRELLRRL
                     ALNDERALSDVIIGSNPLVSDLLDDKTRALVRLAGVITVGPEETSLRAAVDSARGSGA
                     RDEEILGTVISIAPMVGGTIISLALPLLFSAM"
     gene            1075..>2029
                     /locus_tag="VLS86_01765"
     CDS             1075..>2029
                     /locus_tag="VLS86_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSO49248.1"
                     /translation="MTARKWQVAPQGARLPLEDSFDLTDLSVRPPRLPSGSVVRRKLI
                     HRLRSARETDVIALIAPAGYGKTTLLAQWAESEAGPIAWLTLTRADNDESTLLSHLGM
                     ALSRAGLIDSDVSFSLRFGRPLSTSDGLSRIGWALANSQSRGALVIDQAEILKTRVTS
                     RMVQDLIDHLDGRLQLVVASRSSTVIPMAKLRSQGKLLEVGEGDLRLDMGEARQLLEV
                     HGIELDLRAANLIERSEGWPVALSLLALAVDPDTEVPAEARGDDRYLAEYFATEVLAK
                     LSPGRRDFLTKTSFLDRLTGGLCNAVLERDDSDKILRLIAEQ"
BASE COUNT          402 a          656 c          619 g          352 t
ORIGIN      
        1 caccgagagt ccgtgccacg ctcggtacgt ccaaaccgtg ccaagaggcc gaatcgccgc
       61 ccgggccggc gacgccccgg gcggccgaat cgatcgcttc agaaccgctg ctcgtggtca
      121 tgactcagcc gaggagggct gccttctgtg actcgaactc ggcgctggtc aggaccccct
      181 gatcacgaag cgctgccagc ttggagagct cgtcggcggt cgaggtgggg gtggcgacct
      241 cctggatgta ctggcgggtc gcctgctccc tcgctgccat gtctgccacg gcgcgctcct
      301 gcatcccctt gcccctggcg atcagataga tgagggcacc gagccacggc aggagaacca
      361 tgaagatcac ccatcccgcc ttgccccatc cggaaaggtc gtcgctgcgg aagatgtcgg
      421 cgaaaaggct gatcaacaac cagatccaga gaaagaacat aaagaaccac agcatcgacc
      481 agaacaggtc catcaagggc atagcctctc cttccatttg gcgggaccga cttcctcgaa
      541 tcccaagccg acacaacagg acggaacgac agagggcatc ccccgcgatg ggggaaatgg
      601 cgccgatccc cattcaccca ggaaagggga tgcgaaagag ccccgattcc gcgatgttcg
      661 ccaccaagga gagtccagac gaggaaaccc ctttgacctc ggcgcagcgc gagttgcttc
      721 gacgtctcgc tcttaatgac gagagggcac tcagcgacgt gatcattggc tcgaatccgc
      781 tggtctccga tctcctcgac gacaagacac gcgcgctggt gagactggcc ggtgtgatca
      841 cggtcggtcc tgaggagaca tctctccggg cggccgtcga tagtgcccgg ggctcgggag
      901 cgcgggatga ggagatcctg ggcaccgtca tcagcatcgc cccgatggtg ggagggacca
      961 tcatcagcct ggctctgccc cttctgttct cagcgatgta ggtcggctga ggctagggcg
     1021 gccccgcgaa tctgtgcacg tcgtttagac ccgatgatgg ctaggttcac cgccatgaca
     1081 gcgcgtaaat ggcaggtggc gcctcaaggt gcccgcttgc ccctcgaaga cagcttcgac
     1141 ctcaccgacc tgagcgtccg accaccacgg ttgccatccg gttcggtcgt gagacgaaag
     1201 ctgatccatc gcttgcgatc cgctcgcgag acggacgtca tcgcgttgat cgccccggcc
     1261 ggttacggca agacgaccct cctggcgcaa tgggccgaga gcgaagcggg gccgatcgcg
     1321 tggctgactc tcacccgcgc agacaacgac gagtcgactc tgctgtcaca tctcggcatg
     1381 gcgctgtcac gtgccgggct catcgactca gatgtcagct tctcgttgcg cttcgggcgc
     1441 ccactgtcca cctccgacgg attgtcacga atcggatggg ctctcgccaa ctcacagagc
     1501 cgcggcgctc tggtcatcga ccaagccgag atcctcaaga cccgggtcac gagcaggatg
     1561 gtccaggatc tcatcgatca tctcgacgga cgtctacagc tggtggtcgc ttcgaggtcg
     1621 agcacagtca tccccatggc gaagctcaga tctcagggaa agctcctcga ggtcggcgag
     1681 ggcgacctca gactggacat gggcgaggct cgccagttgc tcgaagtcca cgggatcgag
     1741 ctcgatctac gtgcagcaaa cctcatcgag cgcagtgaag gatggccggt cgcgctatcc
     1801 ctcctggccc ttgcagtcga ccccgacacg gaggttcctg ccgaggctcg tggagacgac
     1861 agatatttgg ccgagtattt cgccaccgag gtcctcgcca agctgtcacc gggtcgtcga
     1921 gacttcctca ccaagacctc attcctggac cggctgaccg gcggactatg caacgcggtc
     1981 ctggagcgag atgactcgga caagatcctg cgtctgatcg ccgagcaaa
//
LOCUS       DATITW010000126         1759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_206473, whole genome shotgun sequence.
ACCESSION   DATITW010000126 DATITW010000000
VERSION     DATITW010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206473"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /gene="lgt"
                     /locus_tag="VLS86_01770"
     CDS             complement(<1..546)
                     /gene="lgt"
                     /locus_tag="VLS86_01770"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HSO49249.1"
                     /translation="MTFLASIPSPPISGFEIGPLRVTFYGILIAIGVMLGWRLTVKRY
                     EEKGGDPALAERILIWMVVIGFLGARLAYVSTHLSRFEGEWWKVLAIWEGGLALFGGL
                     TAGALTVIILNRRWGGSLPLFLDSVAPAVPLAQAFGRWGNYFNQELFGTPTNLPWGLE
                     IAPENRPEAYPDATTFHPTFLY"
     gene            654..>1759
                     /locus_tag="VLS86_01775"
     CDS             654..>1759
                     /locus_tag="VLS86_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSO49250.1"
                     /translation="MIRPSGAAIEVPPRSSMESFLDEYQLVRLILSLIGLALIGGFAL
                     SGYGPPQLVPYAIGLAGTGFHAYWCRHNKIRAPRAMLVIDLTLWGSLWLLMADMPVVT
                     TASLAFLVLLAVLFSDGIWMIGFLVYVVGWYGAAYFMGHDLTLETAGGFVSVILTIGG
                     LAMVMYRIRGWLGRLDANRSQMLGTVSHELRNTLTGMMGLTEVVSTATDLGPDETREL
                     IGLAHQQTVDASEIIEDLLTVSRLEQAALSVGVEEVDVNREVATTVRRFVGAGTEVTV
                     GAEVTLPMASGDALRVRQILRNLVSNAVRYGGPAIAVTTLAARAGVQIIVGDDGPGVA
                     REDESTIFLPYRRSSNTPADVASVGLGLWICRQLA"
BASE COUNT          317 a          541 c          583 g          318 t
ORIGIN      
        1 gtagagaaag gtgggatgaa aggtggtcgc atcgggatag gcctcgggcc ggttctcggg
       61 tgcgatctcc aggccccagg gcaggttggt cggggtcccg aacagctcct gattgaagta
      121 gttgccccag cgcccaaatg cctgggcgag ggggacggcg ggagccaccg agtcgaggaa
      181 cagcgggagg ctgccacccc agcggcgatt gaggatgatc acggtcaggg cgccggcggt
      241 gagaccaccg aacagggcca gaccaccctc ccagatggcg aggaccttcc accactctcc
      301 ctcgaatcgg gagagatgag tggagacgta ggcaagtctc gccccgagga acccgatgac
      361 caccatccag atcaggattc gctcggcaag ggccgggtcg cctcctttct cctcgtagcg
      421 tttgaccgtc aaccgccaac cgagcatgac accgatggcg atgaggatcc cgtagaaggt
      481 cacgcgcaac ggcccgatct caaagccgct gatcggaggt gacgggatcg aggcgaggaa
      541 agtcatgacc gacgacaact acaacccatg gcggccgcaa ccatatagcc gccggtgaag
      601 gcgcgccttg gccaaaaact ccagttcggg acaggatcgg tcgatcaata gggatgattc
      661 gcccttcggg cgccgccata gaggtgccgc cgcgttcctc gatggagagc ttcctggacg
      721 agtaccagct ggttcgtctg attctgagcc tcatcggatt ggccttgatc gggggcttcg
      781 ccctcagcgg ctacgggcct ccgcaactcg tcccctacgc catcgggctc gcgggcaccg
      841 ggttccacgc ctactggtgc cgtcacaaca agatccgagc cccacgggcg atgctcgtca
      901 tcgacctgac cctgtgggga tcgttgtggc tcttgatggc agacatgcca gtcgtgacca
      961 cggccagcct cgcttttctc gtgctcctcg cggttctctt ctccgacggg atctggatga
     1021 tcgggttcct cgtctatgtc gtcgggtggt atggagctgc ctacttcatg ggccacgacc
     1081 tcaccctgga gacagccggc ggttttgtct cggtcatcct caccatcggc gggctggcca
     1141 tggtcatgta tcggatccgt ggctggctgg gccgtctcga cgccaatcgc tcccagatgc
     1201 tgggcacggt cagtcacgag ctccggaaca cgctcaccgg gatgatgggg ttgaccgagg
     1261 tggtcagcac cgccaccgat ctcggccctg acgagaccag ggaactgatc gggcttgccc
     1321 accaacagac ggtcgatgcg tctgagatca tcgaggatct gctcaccgtg tcgcgtctgg
     1381 aacaggctgc gttgagcgtc ggggtggagg aagtggatgt gaaccgcgag gtcgcgacca
     1441 cggttcgacg cttcgtgggt gcggggaccg aggtgacggt tggggccgag gtgacacttc
     1501 ccatggcgag tggagacgcg ctccgggtcc gccagatcct gcgcaacctg gtctcgaacg
     1561 cggtccggta cggaggaccc gcgattgcag tcaccacgct ggctgcccga gcgggagtgc
     1621 agatcatcgt cggcgacgat ggcccgggtg tggccaggga agacgagagc accatattcc
     1681 tcccttaccg ccggtccagc aacacccccg ccgatgtcgc ttcggtgggt ctgggcttgt
     1741 ggatctgtcg tcagctggc
//
LOCUS       DATITW010000127         1682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_486799, whole genome shotgun sequence.
ACCESSION   DATITW010000127 DATITW010000000
VERSION     DATITW010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486799"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..240
                     /locus_tag="VLS86_01780"
     tRNA            164..240
                     /locus_tag="VLS86_01780"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:198..200,aa:Trp,seq:cca)
     gene            271..594
                     /gene="secE"
                     /locus_tag="VLS86_01785"
     CDS             271..594
                     /gene="secE"
                     /locus_tag="VLS86_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HSO49251.1"
                     /translation="MNREMRRMQEREERLQKKGDTGRDKANRRAAATQAKTPVAERKS
                     LWARLRAYLHEVRQELRKVTWPTREQMIAFTAVTVITSTVLTAVVFGLDIVMKEFVFW
                     LVARS"
     gene            594..1469
                     /gene="nusG"
                     /locus_tag="VLS86_01790"
     CDS             594..1469
                     /gene="nusG"
                     /locus_tag="VLS86_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005518225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HSO49252.1"
                     /translation="MPDLIETAEVESEEAEAAEADPVEADELEAAEPETTEPEAAEDE
                     IADAEAAGAEPAEVETRDVADAVAEAPAPDVAIADIDTVEIDVVEVDGSGESEESAPP
                     VEAAKPASPYDLPGDWYVIHSYSGYENKVKANLETRIQSMHMGDRIFEVHIPMEDVVE
                     FKAGKKVTVPKKVFAGYILVRMTLDDDSWYAVRNTPGVTGFVAGNAQKPTPLSRREVE
                     RFLGVQEEEEKAKPRFKPEWEVGEQVRVVTGPFADFNGVIEDMNVDQQKVVVLVNIFG
                     RDTPVELGFEDIQKS"
     gene            1544..>1682
                     /locus_tag="VLS86_01795"
     CDS             1544..>1682
                     /locus_tag="VLS86_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HSO49253.1"
                     /translation="MQLPAGQATPAPPVGTALGPHGVNLMEFVKAYNEATASQRGQII
                     PV"
BASE COUNT          338 a          518 c          550 g          276 t
ORIGIN      
        1 ttccctgaac gaatctgaca ccgggctggt tccggtttct atggtctgtg ggaattcggt
       61 ccggtggtca ccggggctcg ggtcagtcac cggctgttct tcccgagcca gagtttccac
      121 accagcggcc gtgccttata cttggtcgtt ccgccaaacc ccgaggggtg tagctcgaat
      181 tggtagagcg ccggtctcca aaaccggagg ttgtgggttc gagtcccccc gcccctgcca
      241 gtctttgatt gagtcgatca ggaagagttg atgaaccgag agatgcgccg aatgcaggaa
      301 cgcgaggagc gcctgcagaa gaagggtgac actggccgtg acaaggccaa tcgtcgcgcc
      361 gcggccacgc aggcgaagac cccggtcgcc gagcgcaagt cactctgggc tcgtcttcgc
      421 gcctacctgc acgaagtgcg tcaggagctg agaaaggtca cctggccgac ccgggagcag
      481 atgatcgcct tcaccgccgt gactgtcatc acctccaccg tgctcactgc ggtggtgttt
      541 ggcctcgaca tcgtgatgaa agagttcgtc ttctggctgg tggcgaggtc ctgatgcccg
      601 acttgatcga gaccgccgag gtcgagagcg aagaggcgga agccgcagag gctgaccccg
      661 tcgaagccga cgaactcgaa gccgcagagc cggaaaccac cgagccggaa gccgcagagg
      721 acgagatcgc ggacgcagag gctgctgggg cggagcccgc cgaggtggag acccgtgacg
      781 tggccgacgc cgtcgccgag gcgccggcgc ctgacgtcgc catcgccgac atcgacacgg
      841 ttgagatcga cgtcgtcgag gtggacgggt cgggagaatc ggaggaatca gcgcctccgg
      901 tcgaagctgc caagccggcc agcccctacg acctccccgg tgactggtac gtgatccact
      961 cctactcggg ctacgagaac aaggtgaagg ccaatctcga gacccgcatc cagtcgatgc
     1021 acatggggga tcggatcttc gaagtccaca tccccatgga ggatgtcgtc gagttcaagg
     1081 ctggcaagaa ggtcaccgtg cccaagaagg tcttcgccgg ttacatcctg gttcgcatga
     1141 ccctcgatga cgactcgtgg tacgcggtgc gcaacacgcc cggcgtgacg ggtttcgtcg
     1201 ccggcaacgc ccagaagcca actccgctgt cccgacgtga ggtcgagcgc tttctcggcg
     1261 tccaggagga ggaggagaag gccaagcctc gcttcaagcc ggaatgggag gtcggcgagc
     1321 aggtgcgcgt ggtcaccggg cccttcgcag atttcaatgg cgtcatcgag gacatgaacg
     1381 tcgaccagca gaaggtcgtg gtcctcgtca acatctttgg ccgcgacacg ccggtcgagc
     1441 tgggcttcga agacattcag aagagttagg cccagcctca ggtcccgggc cccaacacac
     1501 aggacacacc aggggtagaa gaagactcgt aaccacgctc aaactgcagc tgcctgcggg
     1561 gcaggcgaca ccggccccgc cggttggcac cgcgctcggc ccgcatgggg tcaacttgat
     1621 ggagttcgtc aaggcctaca acgaggcgac cgccagccag cgtggccaga taatcccagt
     1681 cg
//
LOCUS       DATITW010000128         1862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_767382, whole genome shotgun sequence.
ACCESSION   DATITW010000128 DATITW010000000
VERSION     DATITW010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767382"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VLS86_01800"
     CDS             <1..396
                     /locus_tag="VLS86_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006823749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSO49254.1"
                     /translation="GGAGQLGRAVKVLDELELDIPVIGLAKRMEEVYLPGRPEPISIP
                     RDAEALYLLQQVRDEAHRFAITYHRNLRSKSMIDSVLDEVPGIGPKRKRDLLKRFGSL
                     KKIREVGPESLAEVVPKNVADDLYAALHS"
     gene            409..1284
                     /gene="rapZ"
                     /locus_tag="VLS86_01805"
     CDS             409..1284
                     /gene="rapZ"
                     /locus_tag="VLS86_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HSO49255.1"
                     /translation="MERVATDHPHVLVVTGMSGAGRSSAAKILEDLGYTVIDNLPPRL
                     LVPAAQSHELPAERHTHLAVVIDSRGGLPIQELRDAVEELGRAGVMTRVVFLDADDDV
                     IVSRYEENKRPHPLGGPTISDSITAERHLLADLREEADVLVDTSSLNVHELRERLTAL
                     FSEEDMTRPMRVSLRSFGFKHGTPRDTDLLFDVRFLPNPYWVPELRHLRGTDERVAEY
                     VMNDDAAKEFVDRIDELLAFLIPRFEAEGKSYLTVGVGCTGGHHRSVAISEELGRRLA
                     QHGVTATVRHRDIDR"
     gene            1281..>1862
                     /locus_tag="VLS86_01810"
     CDS             1281..>1862
                     /locus_tag="VLS86_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HSO49256.1"
                     /translation="MKPQTEIALAPLLLDPEGPRVVAVGGGHGQAAALEAIQCYAGAI
                     SALVSVGDDGGSSGRLSDMGMPPPGDVRRCLLALTPEPSLWSELFSHRFTGGDVAEHS
                     LGNLILAGLTDLFGDFESAVATAERMLGTLGSVIPVADHPITLEATIDGRRVVGQKKI
                     SQTAGEVSELHIEPRDTAATQRALAAVAAANQIV"
BASE COUNT          324 a          610 c          620 g          308 t
ORIGIN      
        1 ggtggggctg gtcagctggg aagggcggtg aaggtcctcg acgagctgga gctcgacatc
       61 ccggtcatcg ggctcgccaa gcggatggag gaggtctacc tgccgggaag acccgagccg
      121 atctcgatcc cgcgtgatgc cgaggccctg tacctcctgc aacaggttcg ggacgaggcg
      181 caccgcttcg ccatcaccta tcaccgcaat ctccgatcca agtcgatgat cgactcggtg
      241 ctcgacgagg tcccggggat cggccccaag cgcaagcgcg acctgctcaa gcggtttggc
      301 tcgctgaaga agatccggga ggtcgggccc gaatcgctgg cagaggtggt ccccaagaac
      361 gtcgcggacg atctgtacgc tgctctgcac agctgaacga gagccccgat ggaacgtgta
      421 gcaaccgatc atccccacgt tctcgtggta accggcatgt cgggcgccgg gcggtcatcg
      481 gcagccaaga tcctcgagga tctcggctac accgtcatcg acaacctgcc gccgcgattg
      541 ttggtcccgg ccgcccagtc gcacgaattg cccgcagagc ggcacacgca cctcgcggtg
      601 gtcatcgact ccagaggcgg tcttcccatt caggagctgc gcgacgccgt cgaggagctg
      661 gggagggccg gggtcatgac cagggtggtc ttcctcgacg ccgatgacga cgtcatcgtc
      721 agccggtacg aggagaacaa gcggccgcat ccgctgggtg ggccgaccat ctccgactcg
      781 atcaccgccg agcggcacct cctcgccgac ctgcgcgagg aggccgacgt gctggtcgac
      841 acctcctcgc tcaacgtcca cgagctgaga gagcggctca cagcgttgtt cagcgaagag
      901 gacatgacca ggccgatgcg agtctcgctt cgctcctttg gcttcaagca cggcacgccg
      961 cgcgacaccg accttctctt cgacgtccgg tttctgccca acccgtactg ggtacccgaa
     1021 ctgcgccatc tgcgtggcac cgacgaacgg gtggcggagt acgtgatgaa cgacgacgca
     1081 gccaaggaat tcgtcgaccg catcgacgag ctcctggcct ttctcatccc cagattcgaa
     1141 gccgagggca agtcgtacct caccgtcggg gtcggatgca ccggtgggca tcaccgctct
     1201 gtggccatat ccgaggaact ggggcgtcgg ctcgcccagc acggggtcac ggccacggtc
     1261 cgtcatcgcg acatcgatcg gtgaagcctc aaaccgagat cgcgctggca ccccttctcc
     1321 tcgaccctga aggaccgagg gtggtggcgg tcggtggcgg ccacgggcag gcagcggctc
     1381 tggaggcgat ccagtgctac gccggggcga tatcggcgct ggtgtcggtg ggcgacgacg
     1441 gcggctcgtc gggacgtctc agcgacatgg gaatgccgcc acctggtgat gtgcgtcgct
     1501 gtttgctggc tctcaccccc gagccgagtc tgtggtcgga gctcttctca catcgtttca
     1561 ccgggggaga cgtggcggag cattcgctgg ggaatctgat cctggccggg ctcaccgatc
     1621 tctttggcga cttcgagtcg gcagtggcca cggcggagcg catgttgggc accctgggat
     1681 cggtcattcc cgtcgccgac catccgatca cgctcgaggc gacgatcgac ggacgacggg
     1741 tcgtggggca gaagaagatc tcgcagacgg ccggcgaggt gtccgagctc cacatcgagc
     1801 cgcgagacac cgcggcgacg caacgggccc tggccgcggt cgccgccgcc aaccagatcg
     1861 tc
//
LOCUS       DATITW010000129         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_46447, whole genome shotgun sequence.
ACCESSION   DATITW010000129 DATITW010000000
VERSION     DATITW010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46447"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VLS86_01815"
     CDS             complement(<1..251)
                     /locus_tag="VLS86_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSO49257.1"
                     /translation="MRQLGWGVIGIGNIVQSTMAPAMVEEAECDLVAAVSRDQGRADE
                     FADRFGARFAYTDYDEMLANPEVEAVFIATPNIFHPEQVV"
     gene            complement(262..1257)
                     /locus_tag="VLS86_01820"
     CDS             complement(262..1257)
                     /locus_tag="VLS86_01820"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSO49258.1"
                     /translation="MPAIDTHAHVTPERYKAAIRDRGEWHGLDSVAGELGRGGFDKSL
                     SERLDEMDELGVDRQLVTPTVGFYQYGNQLEKTKLIARECNDEIADMVRDHPTRFSGL
                     ATLPMQDPASAIAELERSVDELGLEGAIVNDHVAGKTYDQPEFLPFFEAAQELGALLF
                     FHQGGDTCVSHRTKLYKLGNAVGNLTERALVFATLVFGGVLDRFPDLRLLLAHAGGYT
                     PYGVPRMDKVAGAMPGDFDGQMRPPFPGDDGFSQKLAPSAYLGRFYYDCCTYSGPVLR
                     FLIDTVGIDRVVLGTDYPAPMVLHDPVNWVNGLPELTTSEKQAIISTNPMTLLGM"
     gene            1383..>2086
                     /locus_tag="VLS86_01825"
     CDS             1383..>2086
                     /locus_tag="VLS86_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HSO49259.1"
                     /translation="MAELMKHRLQRGERLVVFAVGRMFHHNVIHHLGMQGDFDGFWID
                     VEHGGLTTHDIEIAATAGMAHDLDCFVRVPPTDYATVTRCFESGATGVMAAQITSADH
                     AEEFVRWAKFAPRGRRGLNPLGFDGGFGTVPLAEFAERSNRDTFVAIQIETAQAVEEV
                     DAIAAIDGVDLLFVGPSDLSQAVGVIGDFTGQASMEAVDRVAAACRAHGKQWGAVTPT
                     PGYAATLIDKGCTLISP"
BASE COUNT          356 a          683 c          675 g          372 t
ORIGIN      
        1 accacttgct cgggatggaa gatgttgggg gtggcgatga agaccgcttc gacctcggga
       61 ttggccagca tctcgtcgta gtcggtgtag gcgaaccggg ccccgaatct gtcggcgaac
      121 tcgtcggcgc gaccctgatc ccggctcacc gccgccacca ggtcgcattc cgcctcctcg
      181 accatggccg gcgccatcgt gctctggacg atgttgccga tcccgatcac tccccagccg
      241 agctgtctca ccacaccagg ctcacatgcc gagcagggtc atcgggttgg tggagatgat
      301 cgcctgcttc tcgctcgtcg tcagctcggg gagcccgttg acccagttga ccgggtcatg
      361 aagcaccatg ggagccggat agtcggtgcc gagcaccacc cgatcgatgc cgacggtgtc
      421 gatgaggaat cggagcaccg ggccgctgta ggtgcagcag tcgtaataga agcgacccag
      481 gtaggcgctt ggcgccagct tctgggagaa gccgtcgtcg ccggggaatg gcggccgcat
      541 ctgcccgtcg aaatcgcccg gcatcgcccc ggccaccttg tccatgcggg gcacgccgta
      601 aggggtgtag ccaccggcgt gcgccagaag cagcctcagg tcggggaacc ggtcgaggac
      661 ccccccgaac accagagtgg cgaacaccag ggcgcgctcg gtcaggtttc ccaccgcgtt
      721 ccccagcttg tacagcttgg tgcggtggct gacacaggtg tcgcccccct gatggaagaa
      781 gagcagcgcc cccagctcct gggcggcttc gaagaagggc aggaactccg gctggtcgta
      841 ggtcttgccc gcgacgtggt cgttgacgat ggcgccctcc agccccaact cgtcgaccga
      901 tcgttcgagc tcggcgatgg cgctcgcggg atcctgcatg gggagggtgg ccagcccgga
      961 gaaacgggtc gggtgatccc ggaccatgtc ggcgatctca tcgttgcatt ctcgggcgat
     1021 gagcttggtc ttctccagct ggttcccgta ttgatagaag cccacagtcg gcgtcaccag
     1081 ctgtcggtcc accccgagct cgtccatctc gtcgagacgc tcggacagcg acttgtcgaa
     1141 accgccacga cccagttccc cggcgaccga gtccaggcca tgccattcgc ccctgtctcg
     1201 aatcgctgct ttgtatcgct caggggtgac gtgagcatgg gtgtcgatcg ccggcattga
     1261 atctctcctc gtggagtctc aaagtctctc ccccgcccac gggaaacaga ccgattggcc
     1321 tactacataa ggtgcattat acattctacc gaaccgccgg gttccgagac agaggaggag
     1381 acatggccga gctgatgaag catcgtctgc agcgaggcga gcgcttggtg gtgttcgccg
     1441 tgggacggat gttccaccac aacgtcatcc accacctcgg gatgcaggga gacttcgacg
     1501 gcttctggat cgatgtcgag cacggtggcc tcaccactca cgacatcgag atcgcggcca
     1561 ccgccgggat ggcgcacgac ctcgattgct tcgtcagggt gccacccacc gactatgcga
     1621 ccgtcacccg ttgcttcgag agcggggcca ccggggttat ggcggcccag atcacctcgg
     1681 cggatcatgc cgaggagttc gtgcgctggg cgaagttcgc ccccaggggc cgacgcgggc
     1741 tcaatccact cggcttcgac gggggcttcg gcacggtgcc tttggccgag ttcgccgagc
     1801 gctccaatcg cgacacgttc gtcgccatcc agatcgagac cgcccaagcg gtcgaagagg
     1861 tcgatgccat cgccgccatc gacggggtcg atctcctgtt tgtcggtccc tccgacctga
     1921 gtcaggcggt gggcgtgatc ggggacttca ccggtcaggc atccatggag gcggtggatc
     1981 gggtggcggc cgcgtgccgc gcccacggaa agcagtgggg ggcggtgact cccaccccgg
     2041 gctacgccgc cacgctcatc gacaagggtt gcaccctcat ctcacc
//
LOCUS       DATITW010000130         2899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_607098, whole genome shotgun sequence.
ACCESSION   DATITW010000130 DATITW010000000
VERSION     DATITW010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607098"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VLS86_01830"
     CDS             <1..538
                     /locus_tag="VLS86_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HSO49260.1"
                     /translation="IATAAIRWVEDVDQVVIATPDKDLNQLVIGSRIVTYNRREQKIL
                     DESGVVEKFGIHPESVPDYLALMGDTADGVPGLPGWGAKSAATVLARYPRLELIPESA
                     GDWDLTVRGADKLAATLRENRELAFAYRELTTLRLDVPIEETLSDLEWRGVPRAAFEE
                     FCSEVGFDPETIRVHRWA"
     gene            complement(585..2573)
                     /locus_tag="VLS86_01835"
     CDS             complement(585..2573)
                     /locus_tag="VLS86_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO49261.1"
                     /translation="MTGRTMKETERIQGHVGPGRGPFAAGLIGQKSLDFGSSSRRLAH
                     RLRPQLPRVISVFIAGVISVALSTIGPKVLGRATDLIFAGVVGQRLPAGITREEAIDA
                     ARAQGQNQIADLLSGVDVVPGQGIDFAAVGQVLLVVLALYAGSALLAYLQGFLLNDVV
                     QHTVFRMRSDVEDKLNRLPLRYFDRQPRGELLSRVTNDIDNVAQSLQQTMSQMLTSVL
                     TILFVVAMMFYISPTLALIALVTIPITMVLAGFVMRRSQRQCIAQWRVTGNLNAQVEE
                     TFTGHALVKVFGRRKEVQESFELENQELFQASFKAQFLSVLIMPIMMFIGNLNYVVVA
                     VLGGLRVASGTMTLGDVQAFIQYSRHFTQPVTQVASMVNLLQSGVASAERVFELLDAE
                     EEISDHDRTAKVIDPHGRVRFEDVSFRYDEDRPLIEDLSLSVQPGQTVAIVGPTGAGK
                     TTLVNLIMRFYELDSGRITLDEIDIASMQRADLRSEIGMVLQDAWLFKGTIRDNIAYG
                     RPEATEDEILAASRATFVDRFVHSLPDGYDTVIDDEGGNVSTGEKQLITIARAFLADP
                     ALLILDEATSSVDTRTELLLQHAMAALRTDRTSFVIAHRLSTIRDADLILVMEEGRIV
                     EQGTHDELIALDGAYADLHRSQFTGPGQEPEPGPELAA"
     gene            complement(2570..>2899)
                     /locus_tag="VLS86_01840"
     CDS             complement(2570..>2899)
                     /locus_tag="VLS86_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018254217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO49262.1"
                     /translation="GTNLSGGQRQRLAIARALIRRPDLYLFDDSFSALDLATDARLRR
                     ALKPVTADATVIIVAQRVSTIADAEQIIVLEDGAIVGLGTHRSLLESCPTYVEIVESQ
                     LTTELAS"
BASE COUNT          530 a          902 c          963 g          504 t
ORIGIN      
        1 gatcgccacc gctgccattc gatgggtcga ggacgtggac caggtggtga tcgcgacacc
       61 ggacaaggac ctcaaccaac tcgtcatcgg cagtcgcatc gtcacctaca accggcgtga
      121 gcagaagatc ctcgacgagt ccggcgtcgt cgagaagttc ggcatccacc cggagtccgt
      181 ccccgattac ctggcgctga tgggcgacac cgccgacgga gtgcccgggt tgcccggttg
      241 gggcgccaag tcggcggcca cggtcctcgc ccgatatccc cgactggagc tgatacctga
      301 gtctgcgggg gactgggact tgaccgtgcg gggcgcggac aagctggccg ccaccctgag
      361 agagaacaga gagctggcct ttgcctaccg cgagctgacg acgctccgtc tcgacgtgcc
      421 catcgaggag accctctcgg atctcgaatg gaggggagtg ccccgcgccg cgttcgagga
      481 gttctgctct gaggttggat tcgacccgga gacgatccgg gtccacagat gggcctagtc
      541 gaatcgggcc cggtcagatg ggcctggtcg ggctgggtgc cggctcaggc ggccagctcg
      601 ggcccgggct ccggctcctg accgggcccg gtgaactgtg agcggtggag atcggcgtag
      661 gcaccatcga gggcgatgag ctcgtcgtgg gtgccctgtt ccacgatccg gccctcctcc
      721 atgaccagga tcaggtcggc atcccggatc gtcgacagac gatgggcgat cacaaagctg
      781 gtgcgatcgg tgcgcaaggc ggccatcgcg tgctggagca acagctcggt cctggtgtcg
      841 accgaactgg tcgcttcgtc gagtatcagc agcgccgggt cggcgaggaa tgcccgggca
      901 atcgtgatca gctgcttctc gccggtgctg acgttgccac cttcatcatc gatcacggtg
      961 tcgtagccat cgggaaggct gtgcacgaac ctgtccacga aagtcgcccg tgatgcggcg
     1021 aggatctcgt cttccgtggc ttcgggccgc ccataggcga tgttgtcccg gatggtcccc
     1081 ttgaacagcc aggcgtcctg gaggaccatg ccgatctcgg agcgcaagtc cgccctttgc
     1141 atcgaggcga tgtcgatctc gtcgagggtg atccgccccg agtccaactc gtagaagcgc
     1201 atgatcagat tgaccagcgt cgtcttgccc gccccggtgg gcccgacgat ggccaccgtt
     1261 tgccccggtt gcaccgacaa cgagagatcc tcgatcagcg ggcggtcctc gtcgtatcgg
     1321 aacgagacgt cctcgaacct cacccggcca tgggggtcaa tgaccttggc ggtgcgatcg
     1381 tggtcggaaa tctcctcctc ggcatcgaga agctcgaaaa cccgctcagc ggaagccact
     1441 cccgattgga ggaggttgac catcgaggcc acctgggtga ctggctgggt gaagtgacgc
     1501 gagtactgga tgaacgcctg cacgtcgccg agggtcatcg tgccgctggc gacgcgaaga
     1561 ccaccgagca ccgcaaccac cacgtagttc aggttgccga tgaacatcat gatcggcatg
     1621 atcagaacgg agaggaactg agccttgaag ctggcctgga acagctcttg gttctcgagc
     1681 tcgaacgact cctgcacttc ctttcgccgg ccaaacacct tgaccagcgc atgcccggtg
     1741 aacgtctcct cgacctgggc gttgaggttc cctgtgaccc gccattgcgc gatgcactgg
     1801 cgttgcgatc ttctcataac gaacccggcg agcaccatgg tgatcgggat ggtcaccaga
     1861 gcgatgaggg ccagcgttgg ggagatgtag aacatcatgg cgacgacgaa caggatcgtg
     1921 agcaccgagg tcagcatctg gctcatcgtc tgctgaaggc tctgtgccac gttgtcgatg
     1981 tcattggtga cacgactcaa cagttccccg cgtggctggc ggtcgaagta gcgaagcggc
     2041 agtcggttca gcttgtcctc gacatccgag cgcatccgaa acaccgtgtg ctggaccacg
     2101 tcgttgagga gaaagccctg gagatacgcg agcagtgccg agccggcgta cagggccaaa
     2161 accacaagca gcacctggcc cacggcggca aagtcgattc cttggccagg caccacgtcg
     2221 acacccgaga gcaggtcggc gatctggttt tgaccctggg ctctggccgc gtcgatggcc
     2281 tcctccctgg tgatgccggc cggcaggcgc tgcccgacga ctccggcgaa gatcaggtcg
     2341 gttgcccttc cgaggacctt ggggccaatg gtcgagagcg ccacgctgat caccccggcg
     2401 atgaagactg agatgaccct gggcagctgg ggtcgtagtc gatgggccag ccgtcgcgac
     2461 gaggagccga aatcgagcga cttctggccg atcagcccgg cggcgaaggg gccgcgaccg
     2521 ggcccgacgt gcccctggat ccgctcggtc tccttcatgg tccggccggt catgacgcca
     2581 gctccgtggt caactgcgac tccacgatct cgacgtaggt ggggcaagac tccagcaagg
     2641 agcggtgggt gccaagaccg acaatcgccc cgtcctccag cacgatgatc tgctcggcat
     2701 cggcgatggt cgagaccctc tgggcgacga tgatcacggt ggcatcggcg gttaccggct
     2761 tcagggcgcg tcgcagcctg gcatcggtgg ccaggtcgag ggcggagaag gagtcgtcga
     2821 acaggtagag atccggtctg cgaatcaggg cacgggcaat ggcgagacgc tgccgctgcc
     2881 cacccgacag gttggtgcc
//
LOCUS       DATITW010000131         1990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_767602, whole genome shotgun sequence.
ACCESSION   DATITW010000131 DATITW010000000
VERSION     DATITW010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767602"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /gene="nuoD"
                     /locus_tag="VLS86_01845"
     CDS             <1..1292
                     /gene="nuoD"
                     /locus_tag="VLS86_01845"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HSO49263.1"
                     /translation="PQFSPTDEGAQEISEHESEARLDEQFGRVVDDDYVFDDGIDDDE
                     RMILNMGPQHPSTHGVLRLQIELEGEIIRRTKPIIGYLHTGMEKTAEQLNFSQGGTNV
                     TRMDYLAPFHNELAHALAVERLLEIEVPPRAAAIRVLLTELNRCSSHLVALATNGMDI
                     GAISMMIYGFREREQILAFFEKTTGLRMNHNFIRPGGVAADLPPGWKDDVVDILDELP
                     RRIQQYDDLLQNNPIWVSRTKGVVVISAEEAIAYGITGPPLRAAGVDWDLRKAFPYSG
                     IEQYEFDVPVGENGDVYDRYRVRVDEIHQSLRIVEQVMDSMPKGDYRVDDRKVTPPPR
                     ARIDESMEALIHHFKLFTEGYKVPPGETYVAVESPRGELGCYLVSDGQARPYRMHTRA
                     PSFANLQALPIAMADSLMADTVATIASMDPVMGDVDR"
     gene            1365..1988
                     /locus_tag="VLS86_01850"
     CDS             1365..1988
                     /locus_tag="VLS86_01850"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HSO49264.1"
                     /translation="MSQWSEATEERAREIISRYPQKRSAVMPLLYLAMAEDGHLTNEG
                     MEEVARWVGITPAQVHAVASFYTMYKREPTGRYLISCCTSISCMLLGGDDILHAIEDE
                     SGVPHGETDAHGLLSVEHVECIGACGGAPAVQVNYELIEGVTPERARDLVRWLSGARP
                     EVVNGDEMQELFGGVRSFDWAIKEADGALGPYPAFQPLGTVGSEGDA"
BASE COUNT          366 a          622 c          679 g          323 t
ORIGIN      
        1 agccgcagtt cagcccgacc gacgaggggg cccaggagat ctccgagcac gagtcggagg
       61 ctcgtctcga cgagcagttc ggacgggtgg tggacgacga ctacgtgttc gacgacggga
      121 tcgacgacga cgagcggatg atcctcaaca tgggccccca gcatccgtcg actcacgggg
      181 tcctcagact tcagatcgag ctcgagggcg agatcatccg tcggaccaag ccgatcatcg
      241 gctatctcca caccggcatg gagaagacgg cggaacagct gaacttctcc cagggtggga
      301 ccaatgtcac ccggatggac tatctggccc cgttccacaa cgagttggcc cacgccctgg
      361 cggtggagag gcttctcgag atcgaggtgc cgccgcgggc ggcggcgatc agggtcctgc
      421 tcaccgagct caaccgctgc tcctcgcatc tggtggcgct cgccaccaac ggcatggaca
      481 tcggcgccat ctcgatgatg atctacggct tccgggagcg ggagcagatc ctggccttct
      541 tcgagaagac gacggggctg cgcatgaacc acaacttcat ccgccccggg ggggtcgccg
      601 ccgaccttcc ccccggatgg aaggacgacg tggtggacat cctcgacgag ctgccaagac
      661 gcatccagca gtacgacgac ctgctgcaga acaacccgat ctgggtgtca cggaccaagg
      721 gtgtcgtggt gatctcggcc gaggaggcga tcgcctacgg gatcaccggg ccgccactgc
      781 gtgctgccgg ggtcgactgg gacctgcgca aggcgttccc ctacagcggg atcgagcagt
      841 acgagttcga cgtcccggtc ggggagaacg gcgatgtcta cgacaggtac cgggttcgag
      901 tcgacgagat ccaccagtcg ttgcggatcg tggagcaggt gatggactcg atgcccaagg
      961 gtgactatcg ggtggatgat cgcaaagtga ctccgccgcc cagggcgcgg atcgacgagt
     1021 cgatggaggc cttgatccac cacttcaagc tgttcaccga gggctacaag gtcccaccgg
     1081 gggagaccta cgtcgcggtc gagtcacccc ggggggagct gggctgttat ctggtctccg
     1141 acgggcaggc ccggccctac cggatgcaca ccagggcgcc gtcgttcgcc aacctccagg
     1201 ccctgcccat cgccatggcc gactccctca tggccgacac cgtggccacc atcgcctcga
     1261 tggacccggt gatgggcgac gtcgaccgct gaacagggtc gatcccgtcg gccgggtcgg
     1321 ccggtttcgc tcggattgtg gctgctacct aatctccggc gtcgatgagt caatggtctg
     1381 aggccaccga ggagcgcgcc cgggagatca tctctcgtta cccacagaaa cggtcggctg
     1441 tcatgccgct gctctatctg gccatggcag aggatggcca tttgaccaat gagggcatgg
     1501 aagaagtcgc ccgatgggtc ggcatcaccc cggcccaggt gcacgcggtc gccagcttct
     1561 acaccatgta caagcgagag ccgaccggca ggtacctgat ctcctgctgc acctcgatca
     1621 gctgcatgct gctcggaggc gacgacatcc tccacgcgat cgaggacgaa tcgggtgtcc
     1681 cccatggcga gaccgacgct catggcctgc tctcggtgga gcacgtggag tgcatcggcg
     1741 cctgtggcgg cgccccggcg gtgcaggtga actacgagtt gatcgaagga gtgaccccgg
     1801 agcgggccag ggatctggtg aggtggctca gcggggcccg gcccgaggtg gtcaacggag
     1861 atgagatgca ggagctgttc ggcggggtga ggagcttcga ctgggcgatc aaggaagcag
     1921 acggcgccct cgggccctat ccggcgttcc agccgctggg aacggtgggg agcgagggcg
     1981 acgcttgagc
//
LOCUS       DATITW010000132         1645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_126580, whole genome shotgun sequence.
ACCESSION   DATITW010000132 DATITW010000000
VERSION     DATITW010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126580"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..936)
                     /locus_tag="VLS86_01855"
     CDS             complement(67..936)
                     /locus_tag="VLS86_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSO49265.1"
                     /translation="MYGIVELGGTKSLVGFGTTPDDIGDVLRVATTEPEETLGLLVAH
                     LQRQTGELTGLGIVSFGPLELRVDHPDYGQITNTTKPGWSQTSVLTPFRKDFDLAIGF
                     DTDVNGAALGEGKWGAALGLENFVYLTVGTGIGGGAVSHGRLVGGLVHPEMGHVVVSP
                     LPGDGFGGVCPYHDSCLEGMASGPALEARFGIPADELGAEAGRAVDLVGFYLAQGIRD
                     IVYTLSPERIVVGGGVAKLPGFFESIDTHLSYQLGGYPGHEEHGPGFVVAPGLGDMSG
                     LAGALVLAESAGA"
     gene            1136..>1645
                     /locus_tag="VLS86_01860"
     CDS             1136..>1645
                     /locus_tag="VLS86_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSO49266.1"
                     /translation="MTEKPYTGSDSLMRSLEHEGVDLVFGVPGGAILPAYDPLVDSPI
                     RHVLARHEQGAGHMAEGFAQASGRVGVVMATSGPGATNLITPLQNALMDSTPLVAITG
                     QVPTTAIGSDAFQEAYTTGLAMHCTKHSYLVTDADEIPDIVHQAFHIASTGRPGPVLV
                     DVAKSALQAA"
BASE COUNT          302 a          589 c          485 g          269 t
ORIGIN      
        1 ggcccacgga aggtcgagga agtcgggatt tccgggacgt atctggaggt gtggtgtccc
       61 cacgactcag gcccccgcgc tctctgccag gaccagggca ccggcgagac cggacatatc
      121 ccccagtccc ggagccacca cgaagccggg cccgtgctcc tcgtgacccg ggtacccgcc
      181 caactggtag gagaggtggg tatcgatcga ttcgaagaac ccgggcaact tggcgacccc
      241 tcccccgacc acgatccgtt ccgggctcag ggtgtagacg atgtcgcgga ttccctgcgc
      301 caggtagaac ccaaccaggt cgaccgcccg ccctgcctcc gccccaagct cgtcggcggg
      361 gatgccgaac cttgcctcga gggcgggccc actcgccatg ccctccagac atgagtcgtg
      421 ataggggcag actcccccga atccatcccc gggaagcggg ctcacgacga cgtggcccat
      481 ctctgggtgc accagcccgc cgaccagtcg gccatgggac actgccccgc ctccgatccc
      541 ggtgccaacg gtgagataga cgaagttctc caacccaagg gcggcacccc acttcccctc
      601 cccgagggcg gcgccattga cgtcggtgtc gaacccaatc gccaggtcga agtcctttcg
      661 aaatggcgtg agcaccgagg tttgggacca ccccggtttg gtggtgttgg tgatctgccc
      721 gtagtcggga tgatccacac gcagctcgag gggcccgaac gacacgatcc ccaacccggt
      781 caactccccc gtctgccgct gaagatgagc gaccaggagg cccaaagtct cctccggctc
      841 ggtggtggcg acccgcagga catcgccgat gtcatcgggc gtggtgccaa agccaaccag
      901 gctcttggtg cctccgagct cgacgattcc gtacatcagg gcattcaacc tcaagccaga
      961 cttccctggg accgattggt ctgggagatc ttgacatctc gctgaccagg gtgtttactc
     1021 gtgaggcaca tgaagaccaa cctgcccgcc gccgtagtac tgcttattga gtagcgcgcg
     1081 ccgtcatttg cgtgcgctct accctcccac ccggagggtt ttttgttgaa agaccatgac
     1141 tgaaaaaccg tacaccggat ccgactcatt gatgcgaagc ctcgagcacg agggggttga
     1201 cctcgtcttc ggggtgcccg gaggtgcgat cctccccgcc tacgacccac tggtcgacag
     1261 cccgattcgc cacgtcctgg cgagacacga acagggcgcc ggccacatgg ccgaaggctt
     1321 tgcccaggcc agcggccggg tcggagtggt catggccact tccgggccgg gcgccaccaa
     1381 cctgatcacc cctctccaga acgccctcat ggactcgacg ccactggtcg ccatcaccgg
     1441 tcaggtgccc accaccgcca tcgggagcga cgccttccag gaggcctaca ccaccggact
     1501 ggcgatgcat tgcaccaagc actcctacct ggtcaccgac gccgacgaga tccccgacat
     1561 cgtccaccag gcattccaca tcgcctcgac cggccgcccc ggaccggtgc tggtcgacgt
     1621 cgcgaagtcc gctcttcagg cggcc
//
LOCUS       DATITW010000133         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_166562, whole genome shotgun sequence.
ACCESSION   DATITW010000133 DATITW010000000
VERSION     DATITW010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166562"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VLS86_01865"
     CDS             <1..820
                     /locus_tag="VLS86_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HSO49267.1"
                     /translation="LLAVTALIAAACGDGDGAGDLLASIEDEGVLTVSTDPAYPPQSS
                     LNEDTGEYEGFDIDVATEIAERIGVDIAWEAPAWETITAGSWNGRWDLSVGSMTVTPE
                     RSDVLHFSTPYYYTPAVMAVAADDDSISDLTTDLDGQSIAVCGSCTYDFYLQKDLEIP
                     GESFDYVIDDADIVTTDTDTTALEQLVQGRVVAVMSSATTIQAAIDAGLPIKVVGEPL
                     FYEPLAAAIDGSASLDTDSFVSRVSEIIEEMHEDGTLSELSDKWFGLDLTQKTT"
     gene            888..1751
                     /locus_tag="VLS86_01870"
     CDS             888..1751
                     /locus_tag="VLS86_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HSO49268.1"
                     /translation="MRNRLELLSARIPFRLKLALVWLVIALIVVVLFALADFDVEWML
                     DQFWFIARGLPWTVIICGLAIVLAVVLALLGALGRLSNSAVAQGITSFYTSFFRGTPL
                     IVQMFLIFLALPQLAPSGWPGLRDSLTLTALQAGVIALGFNYGAYMTEIFRAGIQSVG
                     HGQIEAAEALGMTYRQRMRRIVLPQAFRMIIPPTGNEFIAMMKDSALISLLGSVVAQM
                     EIFRRAQLVGRADFRPLEAFVVAAALYWVLTTVFQYFQTRLERRLSAGYVRTVEVSNR
                     PADTVLDGDPA"
     gene            1748..>2036
                     /locus_tag="VLS86_01875"
     CDS             1748..>2036
                     /locus_tag="VLS86_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007497782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO49269.1"
                     /translation="MTEPVVRVRNLRKVFGDNEVLKGIDLDVDRGEVVVVFGRSGSGK
                     STLLRCINFLEEPTSGTVEVGGIRLEPGTRTHRQREAIRSVRLKAGMVFQEF"
BASE COUNT          341 a          653 c          659 g          383 t
ORIGIN      
        1 gcttctcgcg gtcaccgccc tgatcgcggc cgcgtgtgga gacggggacg gtgccggcga
       61 cctcctggcg tcgatcgagg acgagggagt gctcacggtg tcgaccgatc ccgcatatcc
      121 cccgcaatcc tccctgaacg aggacacggg tgagtacgag ggcttcgaca tcgacgtggc
      181 caccgagatc gctgagcgga tcggcgtcga catcgcctgg gaggcgccgg cttgggagac
      241 gatcaccgcc ggaagctgga acggtcgttg ggacctcagt gtcgggtcga tgaccgtcac
      301 cccggagcgt tccgacgtgc tccacttcag cacgccctac tactacacgc cggccgtgat
      361 ggccgtcgcc gccgacgacg actcgatcag cgatctgacg acagacctcg acggtcagag
      421 catcgcggtc tgcggatcgt gcacctacga cttctacctg cagaaagacc tggagatacc
      481 gggtgaaagc ttcgactacg tcatcgacga cgccgacatc gtcaccaccg acaccgacac
      541 cacagccttg gagcaactgg tccagggccg ggtggtggcc gtgatgtcct cggcgacgac
      601 gattcaggcg gcgatcgacg ccggtctgcc gatcaaggtc gttggagagc ccttgttcta
      661 cgagcctctg gccgcggcca tcgacgggag cgcctcgctc gataccgact cgttcgtgag
      721 ccgggtctcg gagatcatcg aggaaatgca cgaggacggc acactgagcg agctgtcgga
      781 caagtggttt ggcctcgacc tgacccagaa gaccacttga acgccgacgg cgagggctgg
      841 cagcgacacc gccgggccac cgccgtcctc gacatgatca agggttgatg aggaatcgtc
      901 tagagctgct cagtgcccgg atcccgttcc ggctgaagct cgccctggtt tggctcgtca
      961 tcgccctgat cgtcgttgtc ctgttcgcat tggccgattt cgacgtcgag tggatgctcg
     1021 atcagttctg gttcatcgct cgcggcctgc cctggactgt catcatctgt ggtctcgcca
     1081 tagtgctggc ggtcgtgctg gcgctgctcg gcgccctcgg gcgtctctcc aacagcgccg
     1141 tcgctcaggg gatcacgagc ttctacacgt ctttctttcg gggcaccccg ctcatcgtgc
     1201 agatgttcct gatctttctc gccctccccc agctggcgcc ttcgggttgg cccggcctgc
     1261 gcgactccct gaccctcacc gcgcttcagg ccggggtcat cgccctcggc ttcaactacg
     1321 gggcctacat gaccgagatc ttcagggcgg gcatccagtc ggtgggacac ggccagatag
     1381 aggccgcaga ggccctgggg atgacctacc ggcagcggat gcggcggatc gtccttcccc
     1441 aggcgtttcg gatgatcatc ccccccacgg gaaacgagtt catcgcgatg atgaaggact
     1501 ccgcgctgat ctctctcctc gggtcggttg tggcgcagat ggagatcttt cgacgcgccc
     1561 aactcgtggg ccgggcggac tttcgaccgt tggaggcatt cgtggtggcc gctgccctct
     1621 attgggtgct caccaccgtg ttccagtact tccagacacg tctcgagcga cgtctgtccg
     1681 ccggctacgt ccgcaccgtc gaggtcagca accggccggc ggacaccgtc ctggacgggg
     1741 atccggcatg accgagccgg tggtcagggt gaggaacctg agaaaggtgt ttggtgacaa
     1801 cgaagtcctc aagggcatcg acctcgacgt ggaccgagga gaggtggtgg tcgtgtttgg
     1861 gcggtcgggg tcgggcaaga gcaccctgct tcgatgcatc aacttcctcg aggagccgac
     1921 atcaggaacc gttgaggtgg gcgggatccg cctcgagccc gggactcgga cccatcgtca
     1981 gcgggaggcc atccgcagcg tccgcctcaa ggcaggcatg gtgttccagg agttca
//
LOCUS       DATITW010000134         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_166597, whole genome shotgun sequence.
ACCESSION   DATITW010000134 DATITW010000000
VERSION     DATITW010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166597"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..577)
                     /locus_tag="VLS86_01880"
     CDS             complement(146..577)
                     /locus_tag="VLS86_01880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HSO49270.1"
                     /translation="MASPMPNDRQVHTTSLKVRFYELDPYDHVNHTNYFSYFETARIE
                     YLTDLGWGLDYLKTQGWQLVVVEVHARFLAAARYGEELTIHTWIEDAGRVRSKWRQTM
                     HRGEEEVARLTVDAAFTDLQGKPRRIPTEFVAGMRKLQGAE"
     gene            622..1638
                     /locus_tag="VLS86_01885"
     CDS             622..1638
                     /locus_tag="VLS86_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSO49271.1"
                     /translation="MKAWQLTDTEGPDSYQLNEVEEPEPEPGQVRIKINVSALNHLDL
                     WLARGLPAPKHLPHTGGADGAGVVDAVGEGTAGFAPGDEIIIDPSFSCGKCTACLNDD
                     IVYCESFQILGEHRPGTFTEKIVIPTINAVRKPEAMSWEVAGSFGLVSATALRMLERA
                     RLRRGETVLVVGVGGGVSAAALHLALAMGAEVYVTSRSPEKIAWAKEEGAVEGFDSAG
                     EFGAEMTEQGGADIVIENVGPATIRQSMRAAKRGGRIAICGSTSGAKMELTIPHLFFR
                     QLELIGSSMGTHGQFARAAEWIGSARAKAPVGKVFDFGDLPSALAYLDSGEQTGKVVL
                     RHPE"
     gene            1642..1869
                     /locus_tag="VLS86_01890"
     CDS             1642..1869
                     /locus_tag="VLS86_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HSO49272.1"
                     /translation="MRLSNILIVVGAGILLVGITMRFFPSLLSWFGHLPGDIRYQREN
                     AQVFIPITSMLVVSVVATVIFNLAARLFGQR"
     gene            complement(1874..>2166)
                     /locus_tag="VLS86_01895"
     CDS             complement(1874..>2166)
                     /locus_tag="VLS86_01895"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HSO49273.1"
                     /translation="SLRPTPSGWYIRLDVNGLPAAPEGSYYEGWVWSDDHEGVSIGTF
                     HFRSGEDPVVLWSGVNVADYPSIWVTLETEDEGPEASDQIMMKGRVEDLVTS"
BASE COUNT          367 a          696 c          709 g          394 t
ORIGIN      
        1 ggtctcggat cgtctggccc tgatgcggga ggggaccatc gtccaaaccg gaaccctgga
       61 ggagatcctc gcccaaccgg ccgatgaatg ggtgaccgag ttcctcgcct agcaaccctt
      121 tctgcgccag aacggtaaaa agggcctact ccgctccctg cagtttcctc atcccagcca
      181 cgaactcggt cgggatccgg cgtggctttc cctggagatc ggtgaaggcg gcgtcgaccg
      241 tgagccgcgc cacctcctcc tcaccacggt gcatcgtctg acgccatttc gacctgaccc
      301 gcccggcatc ctcgatccag gtgtggatgg tcagctcctc cccgtaacgg gccgcggcga
      361 gaaaccgggc atggacctcg accacgacca gctgccaccc ctgggtcttc agatagtcca
      421 gaccccaccc gagatcggtc aggtactcga tgcgggcggt ctcgaagtag gagaagtagt
      481 tggtgtgatt gacgtggtcg taggggtcga gctcgtagaa ccgcaccttg agggatgtgg
      541 tgtggacctg gcggtcattg ggcatcggcg aggccatatc gctcttctcc gcgagaacgg
      601 cgcgaggcta ccctcgacca aatgaaggcc tggcagctga ccgacactga gggaccggac
      661 tcctaccagc tcaacgaggt tgaggagccg gagccggagc cgggtcaggt tcggatcaag
      721 atcaacgtct ccgccctcaa tcatctcgat ctctggttgg cacgtgggtt gccggcaccc
      781 aagcacctgc ctcataccgg tggcgccgac ggggcagggg tggtcgatgc agtcggtgaa
      841 ggcaccgccg ggttcgcgcc cggcgacgag atcatcatcg acccctcctt ctcctgcggg
      901 aagtgcacgg cctgtctcaa cgacgacatc gtctactgcg agtccttcca gattctcggc
      961 gagcaccgac ccggcacttt caccgagaag atcgtgatcc cgaccatcaa cgccgtgcgc
     1021 aagcccgagg cgatgtcctg ggaggtggcg ggatcgttcg ggctggtatc ggccactgcc
     1081 ctgcggatgt tggaacgggc acgcttgcgc cgcggggaga ccgtgctggt ggtgggtgta
     1141 ggcggcgggg tgtcggcggc ggcgcttcat ctcgccctgg cgatgggagc cgaggtctac
     1201 gtgacctccc gctcgccgga gaagatcgcc tgggccaagg aagagggcgc cgtcgaggga
     1261 ttcgactcgg ccggggagtt cggggccgag atgaccgagc agggcggtgc cgacatcgtt
     1321 atcgagaacg tgggcccggc caccatccga cagtcgatgc gggccgccaa gcgcggcggg
     1381 cggatcgcga tctgtggctc caccagtggc gccaagatgg agctaaccat ccctcatcta
     1441 ttcttccgcc agctggagct gatcgggtcg tcgatgggca cccacggtca gttcgccagg
     1501 gccgccgagt ggatcggctc ggcaagggcc aaagcgcccg tggggaaggt gttcgacttc
     1561 ggggatctcc cctcggccct cgcctatctc gactccgggg agcagaccgg aaaggtcgtc
     1621 ctccgtcatc cggagtgagc ggtgcgtctc tccaacattt tgatcgtggt cggggcgggg
     1681 atcctcctgg tcgggatcac catgcggttc ttcccctctt tgctctcctg gttcggccat
     1741 cttccgggcg acatccgcta ccagcgggag aacgcccagg tgttcatccc catcacctcg
     1801 atgctggtgg tgagcgtggt ggcgaccgtg atcttcaacc tggcggcccg cctcttcggc
     1861 caacgctgaa cgatcagctg gtgaccaggt cctcgacccg acccttcatc atgatttggt
     1921 ccgaggcctc ggggccttcg tcctcggtct cgagagtcac ccagatcgac gggtagtcgg
     1981 ccacgttgac gccggaccac aaaaccaccg ggtcctcgcc ggagcggaaa tggaaagtgc
     2041 cgatgctcac gccttcgtgg tcgtcactcc acacccagcc ctcgtaatag ctgccttcgg
     2101 gcgcggcggg gagcccgttg acgtcgagac ggatgtacca ccccgatggt gtcgggcgca
     2161 acgagg
//
LOCUS       DATITW010000135         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_206883, whole genome shotgun sequence.
ACCESSION   DATITW010000135 DATITW010000000
VERSION     DATITW010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206883"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VLS86_01900"
     CDS             <1..1000
                     /locus_tag="VLS86_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSO49274.1"
                     /translation="EVLLAPAVVLATGGAGRIYSQTTNPAGVTGDGIAMAGRAGARLA
                     DLEFVQFHPTALLVGKDPMPLLTEALRGEGAILVDAHGRRFMPQYHPMAELAPRDIVA
                     RAIFWQYDRGLGGAYLDARSIVDFADRFPTVNAHAHSVDLDPSDHLLPVSPAAHYYMG
                     GIDADANGRTSVPGLWAVGEASSTGVHGANRLASNSLLEGLVFGARTSADVATSLVAP
                     DGEPFAPKEAFDLPLVAGPALEDLRQIRWDRVGLVRTGPGLWEARSHLLELDSVLRRT
                     IAGRVALELSLMVILAALRRSESRGGHYRADYPEPDPMQEHRTLSDPTRAQMTAVS"
     gene            1000..1833
                     /gene="nadC"
                     /locus_tag="VLS86_01905"
     CDS             1000..1833
                     /gene="nadC"
                     /locus_tag="VLS86_01905"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HSO49275.1"
                     /translation="MLRADYVEVVGRALSEDLGSEGDITSDSCVPKTAESKGVFVARM
                     PGVIAGIEVARYVFEALDPSVRFEPKVDDGHRVDRGGHVADISGHSRSILSGERTALN
                     LLGRMSGVATATAQLVEAVAGTGARMSDTRKTMPGLRALDKYAVRVGGGMNHRFGLYD
                     AVLIKDNHLVAAGDIAAAVAAARTKVRPGVMIEVEVVSLEQLAQVLQTDADRVLLDNM
                     GPEMLRQAVEMTEGRVTLEASGGVTLENVGLLAETGVDIISVGWITHSAPQLDIALDF
                     M"
     gene            1862..>2089
                     /locus_tag="VLS86_01910"
     CDS             1862..>2089
                     /locus_tag="VLS86_01910"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578853.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HSO49276.1"
                     /translation="MSERPGRVAVTGAAGQIGYSLVFRIAAGDMLGKDQPVILQMLEI
                     PQALGALDGVTMELEDGAFPLLEGTVATDDPE"
BASE COUNT          337 a          670 c          722 g          360 t
ORIGIN      
        1 ggaggtgctt ctcgccccgg cagtggtgct cgccaccggt ggtgcgggga ggatctactc
       61 ccagaccacc aaccccgcag gggtgacagg agacgggata gccatggcag ggcgggccgg
      121 agcgcgtctg gccgatctcg agttcgtgca gttccatccc accgcccttc ttgtcggcaa
      181 ggacccaatg ccgctgctga ccgaggccct gcggggcgaa ggagccatcc tggtcgacgc
      241 ccacggccgc cgtttcatgc cccagtacca ccccatggcc gagctggccc cccgggacat
      301 cgtggcgcgg gccatcttct ggcaatatga ccggggtttg ggcggtgcct atctcgatgc
      361 gagatcgatc gtcgacttcg ccgatcggtt cccgactgtc aacgcccacg cccactctgt
      421 cgatctcgac ccgtccgatc atctgctgcc ggtctcaccc gcggctcact actacatggg
      481 tgggatcgat gcagatgcca acggacgaac gtcggtccct ggtctgtggg cggtgggaga
      541 ggccagctcg accggcgtgc acggggccaa ccggcttgcc tccaactcct tgttggaggg
      601 cctcgtcttc ggggcgcgga cgtcggccga tgtggcgacc tcgctggtag cgcccgatgg
      661 ggagccgttc gcccccaagg aggccttcga tctgccgttg gtggccggcc ccgctctcga
      721 ggacctccgt cagatcaggt gggaccgggt aggcctcgtg cgcacaggcc cgggtctctg
      781 ggaggcgagg agccacctgc tcgagctgga ttccgtcctg cgtcggacga tcgctggtcg
      841 ggttgccctc gaactgtcgc tgatggtcat cctggccgcg cttcggcgct ccgagtcgag
      901 gggcgggcat taccgggccg attacccgga acccgatccg atgcaggagc accgcaccct
      961 gagcgatccg accagggcac agatgacggc ggtgagctga tgcttcgcgc cgactacgtc
     1021 gaagtggttg gccgcgccct gtccgaggac ctcggctctg agggggacat cacttccgac
     1081 tcgtgtgtcc caaagaccgc ggagagcaag ggcgtgttcg tcgcccggat gcccggcgtg
     1141 atcgccggga tcgaggtcgc gagatacgtg ttcgaggcgc tggacccgtc ggtgcgcttc
     1201 gagccgaagg tggacgatgg ccatcgtgtc gacaggggag ggcacgtcgc tgacatatcg
     1261 ggacactcac gttccattct cagcggagag cgaacggcgc tcaacctgtt gggccggatg
     1321 tctggtgtgg ccacggcaac ggcgcaactg gtggaggccg tcgccgggac cggggcgcgc
     1381 atgtccgaca cccggaagac gatgccgggg ctcagggcgc tcgacaagta tgcggtccga
     1441 gtcgggggcg gtatgaacca ccgattcggc ctctacgacg cggtcctgat caaggacaac
     1501 catctcgtcg ccgccggtga cattgccgcc gcggtcgccg cggcgcggac caaggttcgc
     1561 ccgggtgtga tgatcgaggt cgaggtggtc agtctggaac agctcgctca ggtcctccag
     1621 actgacgctg accgtgtgct gctcgacaac atgggcccgg agatgctgcg ccaggcggtc
     1681 gagatgacag agggtcgcgt cacgttggaa gcgtcgggcg gcgtcacgct cgagaatgtc
     1741 ggcctcctgg ccgagaccgg cgtcgacatc atctcggtgg gatggatcac ccattcggcg
     1801 ccacagctcg acatcgcact ggatttcatg tgactctcgg atcggagata acttcgacgc
     1861 gatgagtgaa cgaccaggac gagttgcagt gaccggagcg gccgggcaga tcggctacag
     1921 cctcgtcttc cggatcgccg ccggggacat gctcggcaag gaccagccgg tcatcctcca
     1981 aatgctcgag atcccccagg cacttggcgc gctcgacggt gtgaccatgg agctggagga
     2041 cggcgccttt cccctgttgg agggaaccgt cgctaccgac gaccccgag
//
LOCUS       DATITW010000136         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_367129, whole genome shotgun sequence.
ACCESSION   DATITW010000136 DATITW010000000
VERSION     DATITW010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367129"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VLS86_01915"
     CDS             complement(<1..266)
                     /locus_tag="VLS86_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HSO49277.1"
                     /translation="MTATAERPSTTIFRRPASTTGFWSWLTTVDHKRIGIMYGYAAFS
                     FFLIGGIEALLLRIQLAQANSEFLTAGAYNALFTMHGTTMIFLFV"
     gene            complement(263..1321)
                     /gene="coxB"
                     /locus_tag="VLS86_01920"
     CDS             complement(263..1321)
                     /gene="coxB"
                     /locus_tag="VLS86_01920"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HSO49278.1"
                     /translation="MARTRQYLRRTGTILGVALLLGACSLIEIEDAALTTFEPAGPFA
                     ERIDGLFWPVFWIAAGVFVLVQGAILVAVFLFRDKEGAKEARQLHGSPKLEVLWTVIP
                     ALILAGVAVPTTAAVFDLTECDPGAMEIQVTGHQWWFEYHYPEADITTANVMVIPAGQ
                     EVCAHMTSDDVLHNFWIPSLNGKRYLVPGQTTLLRLEAFEPGEFWGHCAEFCGLSHSL
                     MRARVLALEPSEYEAWVEGQQAPATLPVEGTPEFAGYQVFLNKACIQCHTVRFDDDAA
                     SNILPPEAFNGPELTHFASRSVFAGATLPEEGQSREDGLKEWLADPPSMKPGSFMPNL
                     ALTEQEIDDLIAWLESNE"
     gene            complement(1382..>2195)
                     /locus_tag="VLS86_01925"
     CDS             complement(1382..>2195)
                     /locus_tag="VLS86_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49279.1"
                     /translation="VALALLLGGCGSEEPTPFALVASSNGSIGVGEQRVMFALVDPET
                     DEFLASPDREATVNFRDENGSPIATYPMEFIWTIPDVRGLYVAKLEIPEAGTYQATID
                     AEGFATAGPVGVVAFEDPPLIQPGEQAPPSETRTSAEYPDLSVITSDPDPDPAMYQLS
                     VSDAVTDGTPAVIVFATPAFCVSQTCGPLLDQVKALRPEYDTVDFVHVEIYEDLQVDS
                     PEELTTVAATGEWGLPSEPWVFVVDGTGLVTAAFEGAASDAELRAAIDTVAP"
BASE COUNT          391 a          690 c          744 g          370 t
ORIGIN      
        1 acgaacagga agatcatggt ggtgccgtgc atcgtgaaca gggcgttgta ggcgccagcg
       61 gtgaggaact ccgagttcgc ctgggccagc tggatccgga gcagcaacgc ctcgatgcca
      121 ccgatcagaa agaacgagaa tgcggcgtag ccgtacatga tgccgatccg cttgtgatca
      181 acggtggtga gccaggacca gaacccggtc gtggacgccg gccggcggaa gatggtggtc
      241 gatgggcgct cggcagtggc ggtcattcgt tgctctccaa ccatgcgatc aggtcatcga
      301 tttcttgttc ggtcaaggcc aggttgggca tgaagctgcc cggcttcatg ctcggcgggt
      361 cggccagcca ttccttgaga ccgtcctccc ggctctgccc ctcctcgggg agggtggctc
      421 cggcgaacac actgcgcgag gcgaagtgtg tcagctcagg gccgttgaaa gcctcgggag
      481 gcaggatgtt ggacgccgca tcgtcgtcga aacgcacggt gtggcactgg atgcacgcct
      541 tgttcaggaa cacctggtaa ccggcgaact ccggagtgcc ctctaccggg agcgtggccg
      601 gagcctgttg cccctcgacc catgcctcgt attcggacgg ttccagcgcc aggactcgtg
      661 cccgcatcag ggaatgggag agcccgcaga actcggcgca gtgaccccag aactctcccg
      721 gctcgaaggc ctccaaccgg agcagtgtgg tctgcccggg caccaggtag cgcttgccgt
      781 tgagggacgg gatccagaaa ttgtggagga cgtcatcgga cgtcatgtgg gcgcacactt
      841 cctgtcccgc cggtatcacc atgacgttgg cggtggtgat gtcagcctcc gggtagtggt
      901 actcgaacca ccactggtgt ccggtgacct ggatctccat cgccccgggg tcgcactcgg
      961 tgaggtcgaa cacggcagcc gtggtcggca ctgcgacgcc ggccaggatg agtgccggga
     1021 tcacggtcca gaggacttcg agcttcgggc ttccgtgaag ctgcctggct tccttggccc
     1081 cttccttgtc ccggaacagg aagacggcca ccaggatcgc cccctggacc aggacgaaca
     1141 ccccggccgc gatccagaac accggccaga acaggccatc gatcctctcc gcgaaaggac
     1201 cggccggctc gaatgtggtg agtgccgcgt cctcgatctc gatcagcgaa caagcgccca
     1261 gaagcagcgc gaccccgagg atggtgcctg tgcgacgcag atactgacga gtccgggcca
     1321 tcggctgagc atcataagga ctcgacaaac cggcgaccaa tctcgcctct tgccgggcca
     1381 atcagggtgc cacggtgtcg atcgcggccc tcaactcggc gtcggaggcg gcgccctcga
     1441 aggccgcggt gaccaggcct gtgccgtcga cgacgaagac ccaaggctcg gatggcagac
     1501 cccactcccc ggtggcggcg acggtggtga gctcctccgg ggagtcgacc tggaggtcct
     1561 cgtagatctc cacgtgcacg aagtccaccg tgtcgtactc ggggcgcagc gccttgacct
     1621 gatcgagcag cgggccacac gtctgggaca cgcagaaggc gggcgtggcg aagacgatga
     1681 ctgccggcgt cccatcggtc acggcgtcgg acacactcag ctggtacatg gccgggtcgg
     1741 ggtcggggtc ggatgtgatc accgaaaggt cgggatactc ggccgaggtc ctcgtctctg
     1801 agggaggagc ctgctcacct ggctgaatca acggcgggtc ttcgaatgcg accaccccga
     1861 ccggtcccgc agtggcgaat ccctcggcgt cgatggtggc ctgataggtg ccggcttcgg
     1921 ggatctcgag tttcgccacg tacagtcccc gcacgtcggg gatggtccag atgaactcca
     1981 tgggataggt ggcgatcggc gagccgttct cgtcacggaa gttcacggtc gcctcccggt
     2041 cgggtgaggc caggaactcg tcggtctcgg ggtcgaccag cgcgaacatc accctctgct
     2101 ctccgacgcc gatgctcccg ttgctcgatg cgaccagggc gaacggggtc ggctcttcgg
     2161 acccgcatcc gccgaggaga agggcgaggg cgacg
//
LOCUS       DATITW010000137         1851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_367158, whole genome shotgun sequence.
ACCESSION   DATITW010000137 DATITW010000000
VERSION     DATITW010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367158"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /gene="prcA"
                     /locus_tag="VLS86_01930"
     CDS             complement(<1..506)
                     /gene="prcA"
                     /locus_tag="VLS86_01930"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009375808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HSO49280.1"
                     /translation="MTIPFYVAPEQLVRDKAEFARKGIARGRSIIAVDYADGVLLLAE
                     NPSKALHKISEVYDRIAFAGVGKYNEFETLRKAGIRQADLMGYLYSREDVTAMGLATA
                     FAQTLGAIFTREPKPFEVEVLIAEISEDGSEHRLFRVTYDGTLYDERNFVAIGGKAEQ
                     LTEGLEGL"
     gene            complement(503..1294)
                     /gene="prcB"
                     /locus_tag="VLS86_01935"
     CDS             complement(503..1294)
                     /gene="prcB"
                     /locus_tag="VLS86_01935"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HSO49281.1"
                     /translation="MNRPMGPLPMQAPSPGSSFSDLLDSLGAKPTLALPPGEKAPLSP
                     EGTTVLALRYLDGVVMAGDRRATEGNLVAHRRIRKVFPADKYSAVSIAGTAGLAIEMV
                     RLFQVELEHYEKIEGVRLSLEGQATFLARLVRGQLPMAFQGLVVVPLFAGYDERERRG
                     RLYSYDAVGGRYEEIDFGSTGSGARLAKSFMRTVYRDDLSGGEAAEIAVRALVAASQE
                     DTATGGPDLRRGIYPTVIRISAAGLEELDDSVIEPMAEQAVETIR"
     gene            complement(1291..1479)
                     /locus_tag="VLS86_01940"
     CDS             complement(1291..1479)
                     /locus_tag="VLS86_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HSO49282.1"
                     /translation="MSDRERSQSRGEDKEQSEEQSATRAESEADLEALDDLLDEIDEV
                     LEENAEEFVKNYVQKGGE"
     gene            complement(1485..>1851)
                     /locus_tag="VLS86_01945"
     CDS             complement(1485..>1851)
                     /locus_tag="VLS86_01945"
                     /inference="COORDINATES: protein motif:HMM:NF015119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HSO49283.1"
                     /translation="TWVSPKLRALDLQYHDVDPERSLYQRLVDRGSMRRLFDDSQIRE
                     AAVSPPSRTRAYFRGRCVSEFSDSLVAANWDSLVFDIGESHLKRVPMMEPLRGGADLV
                     GPMIDEAEDAADLLRRLGG"
BASE COUNT          308 a          626 c          563 g          354 t
ORIGIN      
        1 cacagccctt cgagaccttc ggtcagctgc tcggctttgc caccgatggc gacgaagttc
       61 cgctcgtcgt acaacgtccc gtcgtaggta acccggaaca ggcggtgctc cgagccgtcc
      121 tcgctgatct cggcgatgag gacctcgacc tcgaacggct tgggctccct ggtgaagatg
      181 gcccccaggg tctgggcgaa ggcggtggcc agccccatcg cggtgacgtc ctcccgcgag
      241 tagaggtagc ccatcagatc cgcctggcgt atccccgcct tgcgcagcgt ctcgaactcg
      301 ttgtacttgc cgacaccggc aaacgcgatg cggtcgtaca cctcgctgat cttgtgcagc
      361 gccttcgagg gattctcggc gagcaggagc accccgtcgg cgtagtcgac cgcgatgatc
      421 gagcgcccgc gggcgatgcc cttgcgggcg aactccgcct tgtccctgac cagttgctcg
      481 ggtgcgacgt agaacgggat cgtcatcgga tcgtctccac tgcctgctcg gccatgggct
      541 cgatcaccga gtcgtccagc tcctccaaac cggcggcgct gatccgaatc accgtcgggt
      601 agatgcctcg tctcagatcg ggtccgccgg tggcggtgtc ctcctgtgaa gcggccacca
      661 gcgccctgac cgcgatctca gccgcctcgc cgccgctgag atcgtcccgg tagacggtgc
      721 gcatgaacga tttcgcgagt cgcgccccgg acccggtgct accgaagtcg atctcctcgt
      781 agcgaccacc gaccgcgtcg tagctgtaga gacggccccg gcgctctctt tcgtcgtatc
      841 cggcgaacag ggggacgacc accagcccct ggaaggccat cggcaactgg cctcgcacca
      901 gacgtgcgag aaacgtggct tgaccttcga gggagagtct gaccccttcg atcttctcgt
      961 agtgctccaa ctcgacctga aacagacgca ccatctcgat ggcgagccct gcggtgcccg
     1021 cgatcgacac cgccgagtac ttgtcggcgg gaaagacctt ccgaatccgg cgatgagcca
     1081 ccaggttgcc ctcggtcgcc cttcgatcgc cggccatcac caccccgtcg aggtagcgca
     1141 gagcgaggac cgtggtgcct tccggagaga gcggcgcctt ctcccctggg ggaagagcaa
     1201 gtgttggctt ggctcccaac gagtcgagca gatccgagaa actcgacccc gggcttggtg
     1261 cctgcatcgg cagcgggccc atgggtcggt tcactcgccg cccttctgga cgtagttctt
     1321 cacgaactct tcggcgttct cttcgagcac ctcgtcgatc tcgtcgagaa gatcgtcgag
     1381 ggcctcgaga tccgcttcgg actcggccct ggtggcggac tgctcttcgc tctgttcctt
     1441 gtcctcacca cgggactgtg agcgttctct gtcagacatc tggttcatcc tcctaggcga
     1501 cgcaggaggt cggctgcgtc ttcggcttca tcgatcatgg ggccgaccaa atcggcccca
     1561 cctcgaagtg gttccatcat aggaacccgt ttgagatgcg attcaccgat gtcgaagacc
     1621 agtgagtccc agttggccgc caccagtgaa tcggagaact cggagacaca ccttccccgg
     1681 aagtaggccc gggtgcggct tggcgggctc accgccgcct ctcttatctg gctgtcgtcg
     1741 aacagcctcc tcatcgagcc ccgatcgacc aagcgctggt agagactgcg ctcgggatcc
     1801 acgtcgtgat actggagatc gagggcgcgc agcttgggac tgacccaggt c
//
LOCUS       DATITW010000138         1597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_126885, whole genome shotgun sequence.
ACCESSION   DATITW010000138 DATITW010000000
VERSION     DATITW010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126885"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VLS86_01950"
     CDS             complement(<1..192)
                     /locus_tag="VLS86_01950"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HSO49284.1"
                     /translation="MELARVGTYPSLTEAELVKARLEVNGIDALVQADSASGAVPFLE
                     VLEGVRVFVRQADLPEALEI"
     gene            242..1015
                     /locus_tag="VLS86_01955"
     CDS             242..1015
                     /locus_tag="VLS86_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HSO49285.1"
                     /translation="MAAHITSSSNPRIKRLAGLRDRKARDLESVFLVEGMAGIERAMD
                     AGHHPLEVYYDPARHASAPFPTEIELSVEQAALDRASYRGRSQGVIAVFGQFDVGLNA
                     LRLGSDPLLIVVEAIEKPGNLGAILRTADAVGAAAVIAADPGTDPFNPNVIRASTGTL
                     FSVPIAVSDLETAVVWLRESSIRLVAADPDGETPLWSADLTGACALLVGAEHDGLTQA
                     AKAAAGTTVSIPMRGSADSLNASVAAAVLAYEALRQRLA"
     gene            1088..>1597
                     /locus_tag="VLS86_01960"
     CDS             1088..>1597
                     /locus_tag="VLS86_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HSO49286.1"
                     /translation="MSALLQPSFDDLSTPLFDVTFCVLDLETTGGSPLNCAITEIGAV
                     KYRGGELIGTFQTLVDPGLPIPPSITVLTGITEAMVVDAPGIGEALPSFLEFIGDSVI
                     VGHNVRFDLSFLRAAGTQLGYEPLSNDSVDTAGLARRLVRDEVRNLRLQTLSAHFRSP
                     VTPIHRALDD"
BASE COUNT          261 a          569 c          491 g          276 t
ORIGIN      
        1 gatctcgagg gcctcgggaa gatcggcttg gcgcacgaac acccgaaccc cctccaggac
       61 ttcgagaaag ggcaccgctc ccgacgccga atcggcctga accaatgcat cgatcccgtt
      121 cacctcgagc cgggctttga ccagctccgc ctcggtcagc gagggatacg tgcccactcg
      181 agccagctcc atcgggcaat cctacgggcc cgatgggggg tcgctgcgat actcggcgcc
      241 gatggccgcc cacatcactt cgagctcgaa tccgaggatc aaacgactgg cgggtcttag
      301 ggaccgcaag gctcgggacc tcgaatcggt gttcctcgtc gagggcatgg ccggcatcga
      361 gcgagccatg gacgccggtc accatccatt ggaggtgtat tacgacccgg cgcgccatgc
      421 cagtgctccc ttcccgacgg agatcgagct cagcgtggag caagcagcat tggatcgcgc
      481 ctcgtaccgg ggacgctccc agggagtgat cgccgtcttc ggccagttcg acgtcggcct
      541 gaacgccctc cgcttgggat cggacccatt gctcatcgtt gtcgaggcga tcgagaagcc
      601 gggaaacctg ggcgccatcc ttcgcaccgc agacgcggtc ggcgccgcgg ccgtgatcgc
      661 tgccgacccc ggtaccgacc cgttcaaccc caacgtgatc cgggcttcga cagggacgtt
      721 gttctcggtc ccaatcgccg tctccgacct ggagacggca gtggtttggc ttcgggaaag
      781 cagcatccgg ctcgtcgccg ccgaccctga cggcgagacc ccgctgtggt cggccgatct
      841 caccggcgcc tgcgccctgt tggtcggggc ggagcacgat gggctgaccc aagcggcgaa
      901 ggccgcagcc gggaccacgg tgtcgatacc gatgcggggc tccgccgact ccctgaacgc
      961 atcggtggcc gccgccgtcc tcgcctacga agcgcttcgc cagcggctgg cctgagacgc
     1021 ccgcggttcg aggtcggtcc cgacgtgctt gatatcgaac atatgttcgg tctagtctcg
     1081 aatgggagtg tccgccctcc tccagccgag tttcgacgac ctgtcgaccc cattgttcga
     1141 cgtgaccttc tgcgtgctcg acctggagac caccggcggg tcacccctca actgcgccat
     1201 caccgagatc ggggcggtca aataccgggg aggggagctg atcgggacct tccagaccct
     1261 ggtcgatccc ggtctgccca tccccccttc gatcacagtc ctcaccggga tcaccgaggc
     1321 gatggtcgtc gacgcccccg gcatcggcga ggcgctcccc tcgtttctcg agttcatcgg
     1381 cgactcggtg atcgtcggtc acaacgtccg cttcgacctg tcctttctgc gagccgccgg
     1441 cacccagctc ggctacgagc cactctccaa cgattcggtg gacaccgccg gcctcgcccg
     1501 ccgtctggtg agagacgagg tgaggaacct caggctgcag acactctccg cccactttcg
     1561 cagcccggtc actcccatcc accgcgccct ggacgat
//
LOCUS       DATITW010000139         1811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_247051, whole genome shotgun sequence.
ACCESSION   DATITW010000139 DATITW010000000
VERSION     DATITW010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247051"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VLS86_01965"
     CDS             <1..402
                     /locus_tag="VLS86_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HSO49287.1"
                     /translation="DVAHPAPGVVGAIEAADTVIIGPSNPPLSIWPILAVDAISAAVR
                     SHPRVIAVSPLIGGKTLRGPAAMVLADLGLGQGTPGVVASYRGLIDTLIVDSSDVVDV
                     GNVDGVSVVAMDTLISRQVEAERLARGILQL"
     gene            399..1097
                     /gene="cofE"
                     /locus_tag="VLS86_01970"
     CDS             399..1097
                     /gene="cofE"
                     /locus_tag="VLS86_01970"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HSO49288.1"
                     /translation="MTLQILPVAGIGHVAHGDDLSEILADALQPLRPSAGDVLVVTHK
                     VVSKAEGRVVAIEGDEEQFKRALVESEASSIVRRRGDLIIAETKHGFICANAGVDRSN
                     AEPGTMILLPEDPDRSAHRIRLRLERRFGVDIPVIISDTFGRPWRRGLTDVAIGVSGL
                     SAIVDLRGSMDWTGRELEVTEIAVADELASAADLVMGKASGIPAALVRGYEGPRGEGR
                     GADLVRPANEDLFR"
     gene            complement(1170..>1811)
                     /locus_tag="VLS86_01975"
     CDS             complement(1170..>1811)
                     /locus_tag="VLS86_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HSO49289.1"
                     /translation="LTFVNTLANVCEAVGADILDVVQGLGMDHRIGPHFLQPGPGYGG
                     SCFPKDTQALIAVAQDAGYDFELLRAVIEADEEQRRRVADKVRQAAGGGLRGRRVGMW
                     GVAFKAGTDDVRESPAVRIAALLLADGAEVVAYDPEASTDLFPMVDSAIGAAIDADVL
                     LVATEWPEFGEVDMTGVKDVMRGHRVVDARNLLDPKAVRAAGLDYWGLGRPGA"
BASE COUNT          300 a          620 c          598 g          293 t
ORIGIN      
        1 gacgtcgcgc acccggcacc cggtgtcgtc ggagcgatcg aggcggccga cacggtgatc
       61 atcggcccct ccaacccgcc actctcgatc tggccgatcc tcgccgtcga cgcgatcagc
      121 gcggctgtcc gaagccatcc ccgcgtcatc gcggtgagcc cgctgatcgg gggcaagacg
      181 ctgcggggcc ctgccgccat ggttcttgcc gacctcggtc tgggacaggg gacgccgggg
      241 gtcgttgcct cctaccgtgg cctcatcgac actctcatcg tcgattccag tgacgtggtc
      301 gacgtcggca acgtggacgg tgtgtcggtg gtcgccatgg acaccctgat ctcccggcag
      361 gtggaggcag agcgactggc acgaggaata ctgcagctgt gaccctccag atccttcctg
      421 tcgccggcat cgggcacgtg gcccacggcg acgacctgag cgagatcctg gccgacgctc
      481 tccagccgct gcgcccatcc gccggtgacg tgctggtggt gacgcacaag gtcgtatcca
      541 aggcggaggg cagggtggtg gccatcgaag gggacgagga gcagttcaag cgggctctgg
      601 tcgagtcgga ggcctcctcg atcgtccgac gcagaggcga cctcatcatc gccgagacca
      661 agcacgggtt catctgcgcc aacgcaggag tcgaccggtc caacgccgag ccgggaacga
      721 tgatcctgct gccggaagac ccggaccgct cggctcatcg cattcgcctg agactggagc
      781 gccgattcgg ggtcgatatc ccggtaatca tcagcgacac ctttggccgt ccttggcgac
      841 gggggctcac agatgtcgcc atcggtgtct ccgggctgag cgcgatcgtg gatctgagag
      901 gctcgatgga ttggacaggt cgggagttgg aggtgaccga gatagcagtc gccgacgagc
      961 tggcctcggc tgcggacctg gtcatgggga aggcgagcgg gatcccggcc gccctggtcc
     1021 gtggctacga aggcccgcgt ggcgagggcc ggggcgccga cctggtccgc cccgccaacg
     1081 aggacctgtt ccgataggcg tgcctccccc gtaattggga gcgagaggtc cgcatatgac
     1141 ccacgaggct cccaattaca ggcagagggt caggctcccg gtctacccag cccccagtag
     1201 tcgagaccgg cggcacgcac ggccttgggg tcgaggagat tccgggcgtc gaccactcga
     1261 tgccctctca tcacgtcctt caccccggtc atgtcgacct cgccgaactc gggccattcg
     1321 gtggcgacga gcaggacgtc ggcgtcgatg gccgccccga tcgcgctgtc caccatggga
     1381 aacagatctg tcgacgcctc cgggtcgtag gccacgacct cggctccgtc ggcgaggagc
     1441 agagcggcga tccgcaccgc cggcgactcc ctgacgtcat cggtcccggc cttgaacgcc
     1501 acgccccaca tcccgacacg gcggccacga agccctccgc cggcggcctg gcggaccttg
     1561 tccgccactc gccgacgctg ctcctcatcc gcctcgatga ccgccctgag cagctcgaag
     1621 tcgtatcccg catcctgggc gactgcgatc agcgcctggg tgtccttggg gaagcacgat
     1681 ccaccatatc caggccccgg ctggagaaaa tgggggccga tccggtgatc catgcccaat
     1741 ccctggacca cgtcgagtat gtccgcaccc actgcctcgc agacattggc cagcgtgttg
     1801 acaaatgtga g
//
LOCUS       DATITW010000140         1552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_607722, whole genome shotgun sequence.
ACCESSION   DATITW010000140 DATITW010000000
VERSION     DATITW010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607722"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VLS86_01980"
     CDS             <1..740
                     /locus_tag="VLS86_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSO49290.1"
                     /translation="AGIKTSATRDGDVYVVNGSKTFITNGINSDVVITAVKTDPQERH
                     SGMSLLVIEDGMEGFKRGRNLDKIGLHAQDTAELFFDEVRVPVSNRLGEEGMGFSYLV
                     HNLPQERLGLAAASVAHAEVAFGWTLDYIKERQAFGQPIGSFQTVKHLMAEMRTELDI
                     AQGYVDRQIQALNRGELTADDAAKAKWWVTELEKRVIDNCLQLFGGWGYMEEYPIAKA
                     YRDARVQTIYGGTTEIMKEIIGRSLGL"
     gene            759..>1552
                     /locus_tag="VLS86_01985"
     CDS             759..>1552
                     /locus_tag="VLS86_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSO49291.1"
                     /translation="MSEPITFDGRVAVVTGAGGGLGKEYALELARRGARVVVNDLGGA
                     VDGSGSSRGAADVVVDEIREAGGEALASYDSVATREGGAAIVQTALDSYGTVDVVINN
                     AGILRDKSFSNMTLDEVEAVLDVHLKGAFYVSHPAFKVMKERGYGRFVHTSSASGLFG
                     NFGQANYGAAKAGLVGLSNVLAIEGAKYNIKSNAVAPTARTRMTEDILGPFVEMFDPR
                     QVMPMVVYLSSEQNQFTHEVFSVGGGRFARVFIGTNTGWFSGQGVVP"
BASE COUNT          296 a          451 c          535 g          270 t
ORIGIN      
        1 tggcggggat caagaccagt gcgacgaggg acggggacgt ctacgtggtc aacgggtcca
       61 agacgttcat caccaacggg atcaactccg acgtcgtgat cacagcggtc aagaccgacc
      121 cgcaggagcg tcattccggc atgagccttc tcgtgatcga ggacgggatg gagggattca
      181 aacgggggcg gaatctggac aagatcgggc tccatgccca ggacacggcg gagctgttct
      241 tcgacgaggt ccgggttccg gtgtcgaatc ggctcggtga ggaggggatg gggttctcct
      301 acctggttca caacctgccc caggagcgtc tgggcctcgc tgccgcctcg gtggcccatg
      361 ccgaggtcgc ctttggctgg actctggact acatcaagga gcgtcaggcg ttcggtcagc
      421 cgatcggcag cttccagacg gtcaagcatc tgatggcgga aatgagaacc gagctcgaca
      481 tcgcccaggg atatgtcgac cggcagatac aggccctcaa tcggggtgag ctcaccgccg
      541 acgatgcggc caaggcgaag tggtgggtga ccgagctcga gaagcgggtc atcgacaact
      601 gtctccagct cttcggtggg tggggataca tggaggagta tccgatcgcc aaggcctacc
      661 gtgacgccag agttcaaacc atatacgggg ggacgaccga gatcatgaaa gagatcatcg
      721 ggcgttcgct tggtctctag cgagaggaaa cgagcagatt gagtgagcca atcaccttcg
      781 acgggagggt cgcggtcgtc accggagccg ggggcggtct cggcaaggag tacgccctgg
      841 aactggcccg gcggggggca cgggtcgtgg tcaacgactt gggcggcgcg gtcgacggca
      901 gcgggtcctc tcggggcgca gccgatgtcg tggtggatga gatcagagag gccggtggtg
      961 aggccttggc gagctacgac tcggtggcga cgcgcgaggg tggggcggcc atcgtccaga
     1021 ccgcgctcga ctcgtacgga acggtcgacg tcgtcatcaa caacgcgggc atcctccgcg
     1081 acaagtcttt ctccaacatg accctcgatg aggtcgaggc ggttctcgac gttcatctca
     1141 agggtgcctt ctacgtctcc cacccggcgt tcaaggtgat gaaggagcgg ggatacggtc
     1201 gcttcgtcca caccagctcg gcgtccggcc tcttcggcaa cttcgggcag gccaactacg
     1261 gcgcagccaa ggccggcctc gtcggtctct ccaatgtcct cgccatcgag ggcgccaagt
     1321 acaacatcaa atcgaacgcc gtcgccccga ccgcccggac caggatgacc gaggacatcc
     1381 tggggccgtt cgtcgagatg ttcgaccccc gtcaggtcat gccgatggtg gtctacctca
     1441 gctcggagca gaaccagttc acccatgagg tgttcagcgt cgggggcggc aggttcgcca
     1501 gggtgttcat cgggaccaac accgggtggt tctccggtca gggggttgtg cc
//
LOCUS       DATITW010000141         1585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_7013, whole genome shotgun sequence.
ACCESSION   DATITW010000141 DATITW010000000
VERSION     DATITW010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7013"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="VLS86_01990"
     CDS             <1..1217
                     /locus_tag="VLS86_01990"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HSO49292.1"
                     /translation="QFDRVAKLMGLDDNVADRLRVPQKSTVVSFPFRHDTYSDVETLF
                     GYRVQHLTTMGPTKGGIRYAPDVDLGEVAALAMLMTWKCSIVGVPFGGAKGGVRVDPM
                     GLSRAELQRVTRRFTMEIINVIGPETDIPAPDLGTNEQVMAWLMDTYSQHVGHSVPAV
                     VTGKPPALGGSVARREATGRGLMFLVPQAAAVRELDPQNATVAIHGFGNVGRYAAIAG
                     QQMGIRIVAVSDISGAVYNPDGLDVEAVAKWVDENRFLEGYPDADFIDGDRVFGLPVD
                     VLVPAAVQNVLRADNAGEVQAKIVIEGANGPTTLEADVILKEKEVLVIPDILANAGGV
                     TVSYFEWVQDVQKYFWTENEVVARLREIMTRAFSDVHNIALSESVDMRSAALIKGIRK
                     VADAKLVRGIFP"
     gene            complement(1214..>1585)
                     /locus_tag="VLS86_01995"
     CDS             complement(1214..>1585)
                     /locus_tag="VLS86_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HSO49293.1"
                     /translation="RGILDRIDRDHDGRLTITDYKTGKAPPERYALPAFFALKIYALL
                     IRRRTGETPKELRLIYLNGPTLYRLDIDDRQLDAMDGQLRALWNAINRALATRTFPPR
                     PGALCNWCSFQDICPAFAGGV"
BASE COUNT          287 a          488 c          534 g          276 t
ORIGIN      
        1 gtcaattcga tcgtgtcgcc aagctcatgg gtctcgatga caacgtcgcc gaccgtctcc
       61 gggtgccaca gaagtcgacc gtggtcagct ttccgtttcg ccacgacacc tactccgatg
      121 tcgagaccct gttcggatat cgggtgcagc acctcacgac catgggcccg accaagggcg
      181 gcattcgcta tgcgccggat gtcgacctgg gggaggtggc cgccctggcg atgctcatga
      241 cctggaagtg ctccatcgtc ggggttccgt tcggtggggc gaagggtggg gtccgggtgg
      301 accccatggg tctgtcccgg gccgagctgc agcgggtcac tcgtcgattc acgatggaga
      361 tcatcaacgt gatcggcccc gagaccgaca tccccgcccc tgacctcggc accaacgaac
      421 aggtgatggc ctggctcatg gacacctact cacagcatgt gggacattcg gtgccggcgg
      481 tggtgacggg caagcccccg gcgctgggag gcagtgtggc caggagagaa gccaccggcc
      541 gtggcctcat gtttctcgtc ccccaggcgg ccgcggtcag ggagttggac ccgcagaacg
      601 ccacggtggc aattcatggc tttgggaacg tcggccgcta tgccgcaatt gcaggtcagc
      661 agatggggat caggatcgtc gcggtgtcgg acatctccgg cgccgtctac aaccccgacg
      721 ggctcgacgt cgaggcggtc gccaaatggg tcgacgagaa ccggttcctc gagggctatc
      781 ccgatgccga cttcatcgac ggggacaggg tcttcggtct cccggtcgac gtcctggtcc
      841 ccgccgccgt ccagaacgtg ctccgtgcgg acaatgccgg cgaggtgcag gccaagatcg
      901 tcatcgaagg cgccaacggg ccgacgacac tcgaagccga cgtcatcctc aaggagaaag
      961 aggtgctggt catccccgac atcctggcca acgccggagg cgtcaccgtc tcctacttcg
     1021 agtgggtaca ggacgtccag aagtacttct ggaccgagaa cgaggtagtg gcacgtctcc
     1081 gggagatcat gaccagggca ttcagcgacg tccacaacat cgccctcagc gagagtgtcg
     1141 acatgaggag cgcggctctg atcaaaggga tccgcaaggt ggcggacgcc aagctggtcc
     1201 gggggatctt cccctagacg cccccggcga acgccgggca gatgtcctgg aatgagcacc
     1261 agttgcacag ggcgcccggt cgtggaggga aggtccgggt ggcgagagcc cggttgatcg
     1321 cgttccagag ggcgcgtagc tggccgtcca tggcgtcgag ctgacgatcg tcgatgtcca
     1381 ggcgatacag ggtggggcca ttgagataga tcagcctcag ctccttgggt gtctccccgg
     1441 tccgacgtcg gatcaacagc gcgtagatct tcaacgcgaa gaaggcgggg agcgcgtacc
     1501 gctccggggg cgccttgccc gtcttgtagt cggtgatggt gagacgcccg tcatggtcac
     1561 ggtcgattcg atccaggatc cccct
//
LOCUS       DATITW010000142         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_607843, whole genome shotgun sequence.
ACCESSION   DATITW010000142 DATITW010000000
VERSION     DATITW010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607843"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..841
                     /locus_tag="VLS86_02000"
     CDS             188..841
                     /locus_tag="VLS86_02000"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017537287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSO49294.1"
                     /translation="MTHLIDLSHDIESGMITYPGLPGPVISEHLSRSDSRERYEPGTE
                     FSIGKIDMVGNTGTYLDAPFHRFEDGLDLAGLVLEDLADLPGTCIASPGPGIGSDVLD
                     GVEVAGRAVLFATGWDRHWLTEAYGGPDHPFVTAELTDALVEGGAVLVGIDSVNIDDT
                     SGGSRPAHTGLLAAGIPVVEHLTGLDQLVGATFRFFAVPPKVRMLGTFPVRAFAVVE"
     gene            complement(860..>2068)
                     /locus_tag="VLS86_02005"
     CDS             complement(860..>2068)
                     /locus_tag="VLS86_02005"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSO49295.1"
                     /translation="KGAEHCRLQEQFVPGSAIRYEYSPESFTGTELPFAKAVCEAVMD
                     VIEPTTESPIILNLPATVEMSTPNLYGDMIEWFHREIRDRDRIVLSLHPHNDRGTAIA
                     AAEFGVMAGADRIEGTLFGNGERTGNVDVVTLALNLFSQGVDPELDLSDINEYRRIAE
                     HCNQLPVHPRHPYVGDLVYTAFSGSHQDAIKKGFEAMEATGQTLWEVPYLPIDPQDVG
                     RSYEDVIRVNSQSGKGGVAYLLKTEQELILPRRLQIEFTRVIQGITDTTGGEITAAEI
                     WTEFRRHYLAILKPFELLRFEITQNAQGENRTFINAIVRHDDEEVEIHSEGGGSIEAF
                     VNAMIANFDVSCRVLDYHEHSIGAGTDAQAVSYVEIEVGKHTIWGVGIHSDVTTSSLQ
                     AIVSGINRAL"
BASE COUNT          332 a          643 c          682 g          411 t
ORIGIN      
        1 ccgccaacac cacatcttcg ccggcaagct ggatcgcaag gtcctgaact tcggcgagcg
       61 tgccatcgtc aacgcgttca aggtgcccga cggcgacttc cgcgattggg accagatcaa
      121 ggaatgggcg ctcggcatcg ccgaggagtt gaagaccaag gtctgagccg gggcgtacgc
      181 tcccccgatg acgcatctga tcgacctctc ccacgacatc gaatccggga tgatcaccta
      241 tcccggtctg cccgggccgg tgatctccga gcatctgtcg cgttcggact ccagggagcg
      301 ctacgagccg ggaaccgagt tctcgatcgg gaagatcgac atggtcggca acaccggcac
      361 ctatctggac gccccgttcc accggttcga ggatgggctc gacctggccg gtctggtgct
      421 cgaggatctc gccgacctgc ccggaacctg catcgcgtcc ccggggccgg ggatcggttc
      481 cgatgtcctc gatggtgtcg aggtggcggg ccgcgccgtc ctgtttgcca ccgggtggga
      541 tcggcactgg ctcaccgagg cttacggcgg ccctgatcat cctttcgtga ccgctgagct
      601 cacagatgcc ttggtcgagg gcggggcggt gctggtcggg atcgactcgg tgaacatcga
      661 cgacaccagt ggcggaagcc ggcccgccca caccggtctg ctcgccgcgg ggatcccggt
      721 cgtcgagcac ctcaccgggc tcgaccaact cgtcggcgca actttccggt tctttgcggt
      781 gcctcccaag gtcaggatgc tgggcacctt cccggttcgg gcgttcgccg tcgtcgagtg
      841 acctggctgc agagtccggt cagagagccc ggttgatgcc ggacacgatg gcctggagcg
      901 acgaagtggt gacgtcggag tggatgccga ctccccagat cgtgtgcttg ccgacctcga
      961 tctcgacgta gctgaccgcc tgggcgtcgg tgccggcccc tatcgaatgc tcgtgatagt
     1021 cgagcacccg acaggaaacg tcgaagttgg cgatcatcgc gttgacgaac gcctcgatcg
     1081 acccgcctcc ctcgctgtga atctccactt cttcgtcgtc gtgccggacg atggcattga
     1141 tgaaggtgcg gttctcgccc tgggcgttct gcgtgatctc gaatcggagc agctcgaagg
     1201 gcttgaggat ggcgagatag tgacgacgaa actcggtcca gatctcggct gcggtgatct
     1261 cgcccccggt ggtgtcggtg attccctgga tgaccctggt gaactcgatc tgcagtcttc
     1321 ggggaaggat gagctcttgc tcggtcttca gcaggtaggc gacgccaccc ttgcccgact
     1381 gcgagttgac cctgatcacg tcctcgtagg aacgtccgac gtcctggggg tcgatgggga
     1441 gatagggcac ctcccacagg gtttgacctg tcgcctccat cgcctcgaag cccttcttga
     1501 tcgcgtcctg gtgtgacccg gagaacgcgg tgtagaccag atcgcccaca tacgggtgac
     1561 gggggtggac cgggagttgg ttgcagtgct cggcgatcct gcggtactcg ttgatgtcgc
     1621 tgagatcgag ctcaggatcg acgccctgac tgaacaggtt gagggcgagg gtgacgacgt
     1681 cgacgttgcc cgttctctct ccgttgccga agagggtgcc ctcgatgcga tccgcgcccg
     1741 ccatcacccc gaactcggcg gccgcgatgg ccgttccccg gtcgttgtgt ggatgcaggg
     1801 agaggacgat ccggtcccgg tcacgtatct cgcggtgaaa ccactcgatc atgtctccgt
     1861 agagattggg tgtgctcatc tcgacggtcg ccggcaggtt gaggatgatc ggcgactcgg
     1921 tggtgggctc gatgacatcc atcactgctt cgcacacggc tttggcgaag ggcagttcgg
     1981 tcccggtgaa gctctcgggg gagtactcgt atcggatcgc cgaccctggg acgaactgct
     2041 cctggagccg gcagtgctcc gccccctt
//
LOCUS       DATITW010000143         1854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_528026, whole genome shotgun sequence.
ACCESSION   DATITW010000143 DATITW010000000
VERSION     DATITW010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528026"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VLS86_02010"
     CDS             complement(<1..793)
                     /locus_tag="VLS86_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSO49296.1"
                     /translation="MARTVIKGGTVVTANATTTSDVLIDGETVVALAAPGSHDWEANA
                     DKVIDASGKYVIPGGIDVHTHMELPFGGTRAADTFETGTRAAAWGGTTTIIDFAVQTK
                     GQNLMDGFEAWMGKADGNCALDYGFHLIVGDVTEQSLKEMDTALDEGVTSFKMFMAYP
                     GVFYSTDGEIFRAMQKAAENGSTVMMHAENGIAIDVLVEQALARGHSDPIYHGLTRPA
                     ILEGEATHRAIKLAELAGCPLYVVHMSAAEALLELTLARDDGQNAF"
     gene            complement(805..1647)
                     /locus_tag="VLS86_02015"
     CDS             complement(805..1647)
                     /locus_tag="VLS86_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HSO49297.1"
                     /translation="MPNIVRAAIVQTEWTGDKDSMIKKNADYAREAAQQGARVMCFQE
                     LFYGPYFCQVQENEHFEYAEPIPDGPTTKLMQDLAKETGMVLVVPIFEKEQEGFYYNT
                     AAVIDADGSYLGKYRKNHIPHVKGFWEKFYFRPGNMGYPVFDTAVGKVGVYICYDRHF
                     PEGWRALGLNGAKIVFNPSATSRGLSMYLWNLEQPASAVANEYFIGAINRVGKEPLGD
                     NDFYGSSYFVNPRGQIIGDSASDTEEELVIRDLDLDLIDEVRQQWAFYRDRRPDAYGD
                     LVAP"
BASE COUNT          316 a          590 c          567 g          381 t
ORIGIN      
        1 caaaggcgtt ctggccatcg tcccgggcca gggtcaactc cagcaaagcc tccgctgccg
       61 acatgtgcac cacgtacagc gggcaacccg ccagctcggc gagcttgatc gcccggtgag
      121 tcgcctcgcc ttcgaggatc gcaggcctgg tcagcccgtg atagatcgga tcgctatgac
      181 ctctggccag cgcctgctcg acgaggacat cgatggcgat tccgttctcg gcgtgcatca
      241 tcacggtcga gccgttctcg gccgccttct gcatcgcccg gaagatctcg ccgtcggtgg
      301 agtagaagac acctggataa gccatgaaca tcttgaacga ggtgacgcct tcatcgagag
      361 cggtgtccat ctccttcagc gactgttcgg tgacatcacc gacgatgaga tggaacccgt
      421 agtcgagggc gcagttgccg tcggcctttc ccatccaggc ctcgaatccg tccatgaggt
      481 tctgaccctt ggtctgcacc gcgaagtcga tgatcgtggt ggtgccgccc caggcagccg
      541 cccgggtccc ggtctcgaag gtgtccgcgg ccctggtccc gccgaagggc agctccatgt
      601 gggtgtgaac gtcgatgcca cccgggatga cgtatttgcc ggatgcgtcg atcaccttgt
      661 cggcattcgc ctcccaatca tgcgagccgg gagccgcgag ggcgaccacg gtctccccgt
      721 cgatcaggac atccgacgtt gtggtcgcgt tggcggtgac gacagtgcca cccttgatga
      781 cggtccttgc catgactcct cccttcaggg tgcgaccagg tcgccgtagg cgtcgggtcg
      841 tcggtcccga tagaacgccc actgctggcg gacctcgtcg atcaggtcca ggtcgagatc
      901 tctgatcacc agctcctctt cggtgtccga ggccgagtcc ccgatgatct gtccccgtgg
      961 gttgacgaag taggaacttc cgtagaagtc gttgtcgccg agtggctcct tgccgacccg
     1021 gttgatggct ccgatgaagt actcgttggc caccgccgaa gcaggttgct ccaggttcca
     1081 caggtacatc gagaggccgc ggctggtggc ggaggggttg aacacgatct tggccccgtt
     1141 gaggccaagc gcacgccatc cctcggggaa gtgacggtcg taacagatgt agacaccgac
     1201 cttgcccacc gcggtgtcga acaccggata gcccatgttc ccggggcgaa agtagaactt
     1261 ctcccagaac cctttgacgt gggggatgtg gttcttgcgg tacttcccca ggtagctgcc
     1321 atcggcgtcg atcaccgcgg ccgtgttgta gtagaagccc tcctgctcct tctcgaagat
     1381 cggcaccacc aagaccattc cggtttcctt ggccagatcc tgcatcaact tggtggtggg
     1441 gccgtcgggg atcggctcgg catactcgaa atgctcgttc tcttggacct ggcagaagta
     1501 gggcccatag aacagctcct ggaagcacat gaccctggcc ccctgctgcg ccgcctcacg
     1561 ggcatagtcg gcgttcttct tgatcatcga gtccttgtcg ccggtccatt cggtttggac
     1621 gatggcggct ctgacgatgt tgggcattca gggtcctacc tgacgacttg tgctgaaagc
     1681 tagcgcagca ggagaccccc cgcgcccgac cagaggtcgg ggtggtcccc ccgctttccg
     1741 aggggggacc actttcagtc cgttcgctac tactcgccgt tctcccgcag ttcgatggtg
     1801 cggcgcacat aaccggcacc atcggtgtcc atgtcagaga ggttggcaac agcc
//
LOCUS       DATITW010000144         1691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_287738, whole genome shotgun sequence.
ACCESSION   DATITW010000144 DATITW010000000
VERSION     DATITW010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287738"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VLS86_02020"
     CDS             complement(<1..362)
                     /locus_tag="VLS86_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HSO49298.1"
                     /translation="MCGIVGYVGPRQVIDVLMPGLARLEYRGYDSAGVALNHGGSLTV
                     LKQAGRIADLQALVDRERPEGAHTGIGHTRWATHGAPNTGNAHPHLDCHRDVVVVHNG
                     IIENWVSLKKKLVEAGHD"
     gene            complement(678..>1691)
                     /locus_tag="VLS86_02025"
     CDS             complement(678..>1691)
                     /locus_tag="VLS86_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HSO49299.1"
                     /translation="IKLLAARGTKLSDEVERELEDRLRSSGGKTAVGPDIGSRFPDGD
                     ALEQYVGHLVDISRYSLSGLRIALDCANGAAYRAAPMLFQRLKADLMVHGDSPDGTNI
                     NAGCGATHPEMLASVARGRIGLSFDGDADRLIAIDEDGRVANGDVIMAIVARHMKTQG
                     ALKKNLVVATVMSNLGFRKSMAEAEIEIVETKVGDRYVMEALLEHKGILGGEQSGHII
                     FLDKGQTGDGLLTAVRLLDVVAGTGKELRQLRAEAITEYPQVLQNVRVARGSSLTDAT
                     DLWDEVRLVESELGEEGRVLVRASGTEPLIRVMVEAPSEAAARGYADRLASVTESSLR
                     KEG"
BASE COUNT          280 a          577 c          516 g          318 t
ORIGIN      
        1 aaatcatgac cggcctcgac cagcttcttc ttgagggaga cccagttctc gatgatcccg
       61 ttgtgcacga cgactacgtc acgatgacag tcgaggtgag ggtgggcgtt tccggtgttg
      121 ggggcgccat gggtcgccca gcgggtgtgc ccgatcccgg tgtgggcccc ttcgggacgc
      181 tcacggtcga ccagggcctg gaggtccgca atccggccgg cctgcttgag aacggtgagg
      241 gaccccccgt gattgagggc gaccccggcc gagtcgtatc ccctgtattc gagccgggcc
      301 agacccggca tcaagacgtc gatgacctgt ctgggaccga cgtatccgac aattccgcac
      361 atgactcttc ctttcaaggc gagatcggga ggtcagggaa atgcgaccct gctcggcccg
      421 atttcgaggg cgatctggag cggcgtgcgt gaaagccggt atcggcgctc atgggtgggc
      481 tctctgtgcc cgggatcact cccgggtttt gcacccatcc tcacgacctg agctggccgg
      541 gaaggcatcc gccgaatgtt tcgatggcct tcctcctcgt cacctcgtcg ccctggatat
      601 ctggggaacc atccccagac cacggttcga cgaggcctgg cgctatctct atccgcgcac
      661 gcaacctgat gttcgggcta tccctccttc cgcagcgagc tctcggtgac actcgccaga
      721 cgatcagcgt accccctcgc agccgcttcg gaaggggctt ccaccatcac ccggatcaaa
      781 ggctcggttc cggaggctcg aaccaggacc cgcccctctt ctcccagctc tgattccacc
      841 agtcgcactt cgtcccagag gtcggttgca tcggtcagac tcgagccacg ggcgactcgt
      901 acgttctgca agacctgggg gtactccgtt atcgcctccg ccctcaattg gcggagctct
      961 ttgccggtgc cggcgaccac gtcgaggaga cgcaccgcag tgagcaaccc gtccccggtt
     1021 tgccccttgt cgagaaagat gatgtggccc gactgctcgc cgccgaggat gcccttgtgc
     1081 tcgagcaggg cctccatcac gtaccggtcg ccgaccttgg tctcgacgat ctcgatctcc
     1141 gcctcggcca tcgacttccg aaagcccagg ttggacatca cggtggccac gacgaggttc
     1201 ttcttgagtg caccctgggt cttcatgtgc ctggcgacga tggccatgat cacgtcgccg
     1261 ttggcgaccc gaccgtcttc gtcgatcgcg atcagacgat cggcatcccc gtcgaacgag
     1321 agcccgatcc gaccccgggc caccgacgcc agcatctcgg ggtgagtcgc cccgcaaccc
     1381 gcgttgatgt tggtcccgtc gggagagtcg ccgtgcacca tgaggtcggc cttgagccgc
     1441 tggaacaaca tcggcgccgc ccggtaagcg gccccgttgg cacagtcgag ggcgatccgc
     1501 agcccactca ggctgtaccg ggagatgtcg accagatgcc cgacatactg ctcgagggcg
     1561 tcaccgtcgg gaaaacggga accgatgtcc gggcccacgg ctgtcttgcc cccggatgac
     1621 cggagacgat cctcgagctc ccgctccacc tcatcggaga gcttggtccc ccgggccgcg
     1681 agcagcttga t
//
LOCUS       DATITW010000145         1836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_87453, whole genome shotgun sequence.
ACCESSION   DATITW010000145 DATITW010000000
VERSION     DATITW010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87453"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VLS86_02030"
     CDS             complement(<1..294)
                     /locus_tag="VLS86_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49300.1"
                     /translation="MAQRPSRVFADPPFAMRHPDRGDRERLAELMLDAYIGTIDYEGE
                     GIDEAQAEITDYLDGEPLLDSSWVLEDGELLLAAILICKYDDGPLVGYVMTRGA"
     gene            complement(336..1115)
                     /locus_tag="VLS86_02035"
     CDS             complement(336..1115)
                     /locus_tag="VLS86_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HSO49301.1"
                     /translation="MTEATRGPETNQVDALWERFKASADADARERLILHYSPLVKYVA
                     GRVGAGLPRSVDHNDLASYGLFGLIDAIDKFELERGFKFETYAINRIKGAILDELRAL
                     DWVPRSVRSRARQIETAIAELEHSLQRAPTDQELADVLEMQVEDLQGALSEIGRSGIA
                     ALDEFVGADSSTSVGDMLADPAGISPETVFQAEETRHGLVDAINRLPQRERLVVTLYY
                     YEGMTLAEIGEVLGVTESRVCQIHAKTMMSLRNRLGEPSWS"
     gene            complement(1090..>1836)
                     /locus_tag="VLS86_02040"
     CDS             complement(1090..>1836)
                     /locus_tag="VLS86_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HSO49302.1"
                     /translation="EEGLRRIADVERRHARRYLAALSKAGYSKRSMTRKASAVRSFYN
                     DSGRRGSTVTNPFEGVSRPRLDRPLPHALPARTLIHAIEAIDSGTPEGLRDKALVETL
                     YSTGLRISELSALTVDDLGGDMVTVVGKGRKTRRVPIGRPAQEAVRRYLSEGRPQLAG
                     REAGTALWVGIRGGRLDSRGIRRIVKKRVATFPHALRHSFATHLLEGGADLRTVQDLL
                     GHTDLATTQIYTAVTRQHLRGTYDRSHPRA"
BASE COUNT          319 a          598 c          594 g          325 t
ORIGIN      
        1 cgccccccgg gtcatcacgt agccgaccag tgggccgtcg tcgtacttgc agatgaggat
       61 cgcggccagg agcaactcac cgtcctccag cacccaggag ctgtcgagca gcggctcacc
      121 atcgaggtag tcggtgatct cggcctgcgc ctcgtcgatc ccctcgcctt cgtagtcgat
      181 ggtcccgatg taggcgtcga gcatcaactc ggcgagtcgc tcccgatcgc cacggtccgg
      241 gtgacgcatg gcgaacggtg ggtcggcaaa gacccggctc ggtctttggg ccaatgagat
      301 tcgatatcgg tgacgctccg gagagcccaa gagaatcagc tccaggaagg ctcgccgagc
      361 cgattgcgaa gagacatcat cgtcttggca tggatctgac acaccctgga ctcggtgacc
      421 ccgagaactt cgccgatctc ggccagggtc atcccctcgt agtagtagag ggtgaccacc
      481 agacgctcac gctgcggaag ccggttgatg gcgtccacca ggccatgacg ggtctcctcg
      541 gcctggaaca cggtctccgg tgagatcccg gccgggtcgg ccaacatgtc ccccaccgag
      601 gtcgaggaat cggcccccac gaactcgtcg agggcggcga tgcccgagcg tccgatctcg
      661 gagagggcgc cctgaaggtc ttcgacctgc atctcgagca cgtcggcgag ctcctgatct
      721 gtcggcgccc tctgcagtga gtgctccaac tcggcgatgg cggtctcgat ctgccgggcc
      781 cgggaccgaa ccgagcgcgg cacccagtcg agggcgcgca actcgtcgag gatcgcgccc
      841 ttgatccggt tgatcgcata ggtctcgaac ttgaacccgc gctccaactc aaacttgtcg
      901 atggcgtcga tcagaccgaa caacccgtag ctggcgaggt cgttgtgatc gaccgaccgc
      961 gggagaccgg cccccacccg cccggccacg tatttgacca ggggcgagta gtggaggatc
     1021 agacgctcgc gagcatcggc atcggccgaa gccttgaagc gctcccatag ggcatcgacc
     1081 tgattggtct caggccctcg ggtggcttcg gtcataggtc cctctcagat gctggcgggt
     1141 cacggcagtg tagatctggg tcgtggccag atcggtatgt ccgagcagat cctgcacggt
     1201 tcgcaagtcg gcgccaccct ccaggaggtg ggtggcgaaa ctatggcgaa gggcgtgtgg
     1261 gaaggtcgcg acccttttct tgacaattct ccgaatccca cgtgaatcta gccgaccacc
     1321 cctgattccg acccacaacg cggttcctgc ctcccttccg gcgagctggg gtctcccctc
     1381 actcaggtag cgcctcaccg cttcttgggc gggacggccg atcggaactc tgcgggtctt
     1441 cctccctttc ccgacgacgg tgaccatgtc cccacccagg tcgtcgaccg tcagcgccga
     1501 cagctcggag atgcgaagcc cggtcgaata cagggtctca accagtgctt tgtcccgcag
     1561 tccttccggg gttcccgagt cgatggcctc gatggcatgg atcagggtgc gggcgggcag
     1621 ggcgtgagga aggggtcggt cgagccgggg gcgagagacg ccttcgaacg ggttggtcac
     1681 cgtcgaaccg cgccgccccg aatcgttgta gaaggagcga accgccgatg ccttgcgggt
     1741 catcgatcgc ttcgagtagc cggccttgga caacgcggcg agatagcgcc gggcgtggcg
     1801 acgctcgacg tcggcgatcc ggcggagacc ctcttc
//
LOCUS       DATITW010000146         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_648287, whole genome shotgun sequence.
ACCESSION   DATITW010000146 DATITW010000000
VERSION     DATITW010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648287"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..468)
                     /locus_tag="VLS86_02045"
     CDS             complement(10..468)
                     /locus_tag="VLS86_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSO49303.1"
                     /translation="MKLPGGEERTVEVLLVEDSPGDVRLTREAFRDADVSIRLHVASD
                     GEEAMAFLRREGPSNVHAPRPDLILLDLNLPKMDGREVLAQVKEDPGLKTIPTVILTT
                     SEAEVDILKSYELQANAYLSKPVQLEAFDSLVKSISDFWLTQVKLPQRTD"
     gene            complement(465..1601)
                     /locus_tag="VLS86_02050"
     CDS             complement(465..1601)
                     /locus_tag="VLS86_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSO49304.1"
                     /translation="MNRLAPDDNWSADLKESEARYRGLLEAAPDAMVVVNQAGEIVLL
                     NVQAESQFGYHRDELLGQPVTNVIPQGFAERLIADDLRSAEDALAQQIGTGIELTALR
                     KDGTEFPIEIMLSPLDSPEGILVTAAIRNITVRKSAEEQLIQSLEELNRSNEELGQFA
                     YIASHDLQEPLRMVASYTQLLARRYKGQLDADADEFIAFAVDGASRMQRLIEDLLSFS
                     RVGTKGQPMAPTSSEGALQQALSNLDVAMSDSGAQVTYDPLPTVLADEIQLVQLFQNL
                     VDNAIKYRRPGVPMIHVSSSDNGDHTWRFSVKDNGLGIEPKYFERIFGMFERLHQRDE
                     FAGTGIGLAICKKIVERHGGRISVESQPGEGSTFHFGLMGAEAT"
     gene            complement(1598..1912)
                     /locus_tag="VLS86_02055"
     CDS             complement(1598..1912)
                     /locus_tag="VLS86_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HSO49305.1"
                     /translation="MAEPTWRDAAACREWTGVDFFPFSEDAVAIRRVKDVCALCPVAD
                     ECLSYAIETRQTDGVWGGHTPKERSYLRRKWMEETRSLNETQRGIALTKAVSLRREKA
                     SS"
     gene            complement(2206..>2713)
                     /locus_tag="VLS86_02060"
     CDS             complement(2206..>2713)
                     /locus_tag="VLS86_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49306.1"
                     /translation="GWQVPGSRSQVNELWLNWYRSWVYGLGFGWQIGTGVVTYLMTAG
                     VYLLILAGALTGSPVVALAGGLAFGSIRGIAVLAGAGISSQARLNSFHRRFESLRRPA
                     ELVTITGLVLVATIAAIGSGDLRLMGVAAVLGATILWAGFKAGPVAGPRKRTDIPLVA
                     TTGQTSEH"
BASE COUNT          508 a          876 c          819 g          510 t
ORIGIN      
        1 tgtagctgat cagtcggtcc tctgcggcag cttgacctgg gtcaaccaga agtcactgat
       61 gctcttcacc aggctgtcga acgcctccaa ctgcaccggc ttgctcaagt aggcgttggc
      121 ttggagctcg tagctcttca agatgtcgac ctccgcctcg gaggtggtga ggatgacagt
      181 ggggatcgtc ttcagtcctg gatcctcctt gacctgagcc aagacctccc gtccatccat
      241 cttggggagg ttcaggtcga gcaggatcag gtcgggacgg ggagcgtgaa cattgctggg
      301 cccttcccgt cggagaaagg ccatggcctc ctcgccgtcc gaagccacat gcagtcggat
      361 cgacacgtcg gcatcccgga acgcctcccg ggtcagccga acgtctccag ggctgtcttc
      421 gaccagaagc acctcgacgg tcctttcctc tcctcctggc agcttcatgt cgcctccgca
      481 cccatcaggc cgaaatgaaa agtggagcct tccccgggct gggactcgac cgagatccgg
      541 cctccgtggc gctcgacgat cttcttgcag atggcgagac cgatcccggt gccggcgaac
      601 tcgtctcgct ggtggagtcg ctcgaacatg ccaaagatcc tctcgaagta cttgggctcg
      661 atcccaagcc cattgtcttt caccgagaac ctccaggtgt gatcaccatt gtcggaagac
      721 gagacgtgga tcatcggcac ccctggccgc cggtacttga tcgcgttgtc aaccaaattc
      781 tgaaacagct ggaccagctg gatttcgtca gccaggacgg tgggcaaggg gtcgtaggtc
      841 acctgcgccc cgctgtcgga catagcgaca tcgagattgg agagggcctg ttgcagcgcc
      901 ccctcgctcg aagtgggggc catgggttgg cctttggtcc cgacgcgtga gaaggagaga
      961 aggtcttcga tcagccgctg catgcgactg gcgccgtcga cggcaaaggc gatgaactcg
     1021 tcggcgtcgg cgtcgagctg gcctttgtat cgtctggcca ggagctgggt atagctggcc
     1081 accatccgca acggctcctg caggtcgtgc gaggcgatgt aggcaaactg ccccagctcc
     1141 tcgttggacc ggttgagctc ctccaggctc tgtatcagct gctcctcggc tgacttgcgc
     1201 accgtgatgt tgcggatggc tgcagtgacc aggatcccct ccgggctgtc caaagggctc
     1261 aacatgatct cgatcgggaa ctcggtccca tccttgcgca gggcagttag ctcgatcccg
     1321 gtcccgatct gctgcgccag ggcatcctcg gccgagcgca ggtcgtctgc gatcaaacgc
     1381 tccgcaaacc cctgcggaat cacgttggtc accggctgcc cgagaagctc gtcacggtga
     1441 tatccgaact ggctctcggc ctggacgttc aacaagacga tctcgccggc ctggttcacc
     1501 accaccatcg catccggagc cgcctccaac agaccccgat acctggcctc ggactccttc
     1561 agatcggcgc tccagttgtc gtctggagca agcctgttca cgaagatgct ttctccctgc
     1621 gcagggagac tgccttggtc agggcgatgc cacgctgggt ttcgttgagc gaccgcgtct
     1681 cctccatcca cttcctgcgg aggtacgacc tctccttggg ggtgtgacca ccccacaccc
     1741 cgtcggtctg gcgggtctct atggcatagc tgaggcactc gtcggcaacg gggcacagag
     1801 cgcatacgtc cttgaccctg cgaatcgcca ctgcgtcctc agagaaggga aagaaatcga
     1861 caccggtcca ctcacggcag gccgccgcgt ctcgccaggt cggttcagcc atttgctcat
     1921 atggctgatt gggggctcgt cggtctaatc gagtcgagat cggggaagaa ttggtaagca
     1981 catcaaggcc ttttcatact tcccgagggg aaccaatcct cggaacgctt ctcaaagacc
     2041 ttcgacggcg ggagagttcc tgcccgcaca accgatagac gaaatgactc gatcggccca
     2101 ggtgggccgg gctcgggtca acgccagata aaccatagca gggcgctcta aaagtcaaca
     2161 gggaggggac gttttaaatc cgccggggcg ctccccggcg acagatcagt gctctgaggt
     2221 ctgaccggtg gtcgcgacca gcgggatatc ggtccgcttc cgggggccgg ctacgggacc
     2281 ggctttgaag ccggcccaca agatcgtcgc gccgaggacg gcggcgacgc ccatcagacg
     2341 cagatcacca cttccaatgg cggcgatcgt ggccaccagg accagcccgg tgatggtcac
     2401 gagctccgcc gggcgccgaa gcgattcgaa gcgacggtgg aagctgttca gcctggcctg
     2461 gctgctgatc ccggcgccgg ccaggactgc gatcccacgg atcgagccaa acgccagacc
     2521 tcccgccaat gcgacgaccg ggcttccggt gagggctccg gccagtatca agaggtagac
     2581 gcccgcagtc atcagatagg tcacgacacc ggtgccaatc tgccacccga acccaagccc
     2641 gtagacccag gaccggtacc agttcagcca gagctcgttc acctggctgc ggctacctgg
     2701 cacctgccac ccg
//
LOCUS       DATITW010000147         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_568278, whole genome shotgun sequence.
ACCESSION   DATITW010000147 DATITW010000000
VERSION     DATITW010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568278"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VLS86_02065"
     CDS             complement(<1..1045)
                     /locus_tag="VLS86_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HSO49307.1"
                     /translation="MSTEREISVDYLARVEGEGAMYLKLKGEEVLDLKFKIFEPPRFF
                     EAFLRGRKFSEAPDITARICGICPIAYQTSACNAMESAAGVQLTGGLRDLRRLIYCGE
                     WIESHALHVYFLHAPDFLGYDNAIQMAADFPEQVQMGLRLKKLGNQIMSLVGGREIHP
                     INIRVGGFYRVPTEEEMKSLVPDLEWALEAAVATASWVAGFPFPDFERDYEFVALRHP
                     DEYAIHEGRIVSNRGLDIGVAEWSDYFEESQVPWSNALHARRKDGGSYHLGPMARYSL
                     NSEQLTPAAMQAAQEAGLGPTCTHPFRSIVVRAVEIAFACEEALRLIRGYRRPESPSL
                     VVEPRDVVGHGASE"
     gene            complement(1035..1811)
                     /locus_tag="VLS86_02070"
     CDS             complement(1035..1811)
                     /locus_tag="VLS86_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HSO49308.1"
                     /translation="MTPARPRLAVWKFASCDGCQLTLLDAEDHLLEVAGAIEIAYFPE
                     ATRGEIEGVYDLSLVEGSITTPHDAERIRAVRQMSKAVVTIGACATAGGIQALRNWEN
                     VDEFLSIVYAHPEYIETLATSTPIAEHIEVDFELRGCPISKYQLLEVIDAFLHRRKPN
                     VPRHSVCVECKLRGTNCVMVAQGIPCMGPITQAGCGAICPAYDRGCYGCYGPSQSPNV
                     GSLAAWLQRLGVPDVDLVRALRTFNAYSPEFREASEVYEH"
     gene            complement(1808..>2372)
                     /locus_tag="VLS86_02075"
     CDS             complement(1808..>2372)
                     /locus_tag="VLS86_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HSO49309.1"
                     /translation="DGVGIRGPYGMGWPVGEAVGKDLVVVAGGIGLAPLRPVVLDVLA
                     RRDRFGAVSIIYGARSPGDLLFEDELRSWRSRFDVETEVTVDRSDDDWFGDVGVVTGL
                     LPRISFDPSNTVAMLCGPEIMMKVVANEMAARGVGAEHLHVSVERNMKCAIGFCGHCQ
                     FGRDFMCRDGPVLPYARVAERLRVSEL"
BASE COUNT          444 a          787 c          739 g          402 t
ORIGIN      
        1 cttccgaggc cccgtgaccg accacatccc gcggctcgac gacgagtgac ggggactctg
       61 gccggcggta gccccggatc aatcgcaatg cctcctcaca ggcaaaggcg atctccacgg
      121 ctcggacgac gatgctccgg aaggggtggg tgcaggtggg gccgagcccg gcctcttggg
      181 cagcctgcat ggcggccgga gtcagctgct cggagttgag cgagtagcgg gccatcggcc
      241 cgaggtgata cgaccccccg tccttgcgcc gcgcatgcag ggcgttggac cacgggacct
      301 ggctctcctc gaagtagtcc gaccactcgg ccacgccgat gtccaaaccg cgattcgaca
      361 cgatcctccc ctcgtggatc gcgtactcat cgggatggcg aagagctacg aattcgtaat
      421 cccgctcgaa atcggggaag gggaagccgg caacccacga cgcagtggcg accgcggctt
      481 ccagcgccca ctccaggtcg ggcaccaggc tcttcatctc ctcttcggtg ggtacccggt
      541 agaagccgcc gacccggata ttgatcgggt ggatctcccg gcccccgacc agggacatga
      601 tctggttgcc gagcttcttc agccggagac ccatctgcac ctgctcgggg aagtcggccg
      661 ccatctgaat tgcgttgtcg tatccgagga agtcgggggc atggaggaag tagacgtgga
      721 gggcatgact ttcgatccac tcgccgcagt agatgagacg gcgcagatcg cggagcccgc
      781 cggtcaactg aacccctgcg gccgactcca tcgcgttgca ggcactggtc tgataggcga
      841 tggggcagat gccgcagatt cgggcggtta tgtcgggcgc ctcggagaac ttgcgcccgc
      901 gaaggaacgc ctcgaagaag cgtggtggct cgaagatctt gaacttgaga tccaggactt
      961 cttccccctt caacttgaga tacatcgccc cctcgccctc cacccgggcc aggtagtcga
     1021 ctgagatctc tcgttcagtg ctcatagacc tcgctcgcct ctcggaactc aggggaatac
     1081 gcgttgaagg tgcgaagggc ccggaccagg tcgacgtcgg ggaccccgag ccgttgcaac
     1141 caggcggcga gggatccgac attgggggat tgggagggcc cgtagcagcc gtagcaaccc
     1201 cggtcatagg cggggcagat ggccccgcac cccgcctggg tgatcggtcc catgcaggga
     1261 atcccctggg caaccatgac acagttggtg ccccggagct tgcactcgac acagacactg
     1321 tgccggggga cgttgggctt tcgccggtgg agaaaggcgt cgatcacctc gagcagctga
     1381 tacttggata tcgggcagcc acggagctcg aaatcgactt cgatgtgctc tgcgatgggt
     1441 gtggaggtcg ccaaggtctc gatgtactcg ggatgggcat agacgatgct cagaaactcg
     1501 tctacgttct cccagttccg caacgcctga atcccacccg cggtggcgca tgccccgatg
     1561 gtcaccacgg ccttcgacat ctgcctgaca gctcggattc gttcggcgtc atgcggggtc
     1621 gtgatcgagc cctccaccag tgagaggtca tagaccccct cgatctcgcc gcgcgtggcc
     1681 tcggggaagt aggcgatctc gatcgcgccg gcgacctcca agaggtggtc ctcggcgtcg
     1741 aggagtgtca gctggcaccc gtcgcacgag gcgaatttcc aaacggcgag tctgggcctt
     1801 gccggagtca cagctctgac acccgaagcc gctccgccac ccgggcgtag ggcagcaccg
     1861 gcccgtcgcg acacatgaag tcccgaccga attggcagtg gccgcagaac ccgatggcgc
     1921 acttcatgtt ccgctccaca ctcacatgca ggtgctcggc gcccactcca cgcgccgcca
     1981 tttcgttggc cacgaccttc atcatgatct cggggccaca gagcatggcg acggtgttgg
     2041 aggggtcgaa cgagatcctc gggagcaggc cggtgaccac acctacatcg ccaaaccagt
     2101 cgtcatccga ccgatcgaca gtgacctcgg tctcgacgtc gaatcgactc cgccatgagc
     2161 gcagctcgtc ctcgaagagc aggtcacccg gcgatcttgc gccatagatt atcgaaaccg
     2221 ctccgaaccg atcccgacgg gccaacacgt ccagcaccac cggacgaagc ggcgccaggc
     2281 cgatacctcc agccaccacc acgagatcct tgcctacggc ttctccgacc ggccatccca
     2341 tcccgtacgg gcctctgatg cccacgccgt cc
//
LOCUS       DATITW010000148         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_448055, whole genome shotgun sequence.
ACCESSION   DATITW010000148 DATITW010000000
VERSION     DATITW010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448055"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VLS86_02080"
     CDS             complement(<1..318)
                     /locus_tag="VLS86_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49310.1"
                     /translation="MSRVYDVHAHVVPEALVDLIRADGHRFGIELVEGEKGLAVMFNG
                     RVTAGPLRRSLTDRGPRLESMDAAGIDVQVLANWVDMTGYALEGPSGAGYSRRLNEII
                     AEEA"
     gene            complement(315..2024)
                     /locus_tag="VLS86_02085"
     CDS             complement(315..2024)
                     /locus_tag="VLS86_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HSO49311.1"
                     /translation="MITDWDVPIPMSDGLVLRADVYRPDTGSHPVIVSYGPYAKGLPF
                     QVGYPDQWNAMVGGHPDVSAGSSNKYQSWEVVDPEKWVPEGYVCLRIDSRGAGRSPGF
                     IDHFSPRETRDLYESIEWAAAQPWSTGRIGLNGVSYYGINQWQAASLRPPSLAAMCVW
                     EGASDHYRDMTHHGGIICTFFANWYDKQVKTVQHGLGTRGPASSINGLQVCGDEDLSD
                     EDLAGNRVDLGAAIASHPFDDQYHRDRSAVFEQITTPLLSAGNWGGHGLHLRGNVEGF
                     MRAGSTQKWLELHGLEHWTHFYTDYGRLLQLEFFDHFLKDEDNGWDQRPPVMLNVRHV
                     DHFEPRTAVSWPLPETKWTRLYLDPDMGLGNEPVDGEWQVSFPAMGDGLTFLTEPYPT
                     EVEITGPSSARLRISSSTEDADLFIVLRLFDPAGDEVTFQGALDPHSPLAHGWLRASH
                     RRTDPDRSTEWRPYHQHTDREALIPGVAYDLDIEIWPTSIVVPAGHRLGVTIRGKDYE
                     GTAPAGSISTFKNPFTGVGPFLHDDPGDRPPDVFAGVTTIHGNGESASILLPFIPRAD
                     VSR"
BASE COUNT          362 a          658 c          734 g          379 t
ORIGIN      
        1 ggcctcttcg gcgatgatct cgttgagacg ccggctatag ccggccccgg aagggccttc
       61 gagggcgtag ccggtcatgt cgacccagtt ggccaggacc tggacgtcga tgcccgcagc
      121 atccatggac tcgagtctcg ggccgcggtc ggtcagcgac cgtcgcaagg ggccggccgt
      181 caccctcccg ttgaacatca cggcgaggcc tttctcacct tccacgagct cgattccgaa
      241 tcggtgaccg tcggcccgga tcagatcgac cagagcctcg gggacgacgt gggcgtgaac
      301 gtcgtagacc cggctcatcg gctcacgtcg gcccgcggga tgaaggggag gaggatcgat
      361 gcgctctcgc cgttcccgtg aatggtggtg accccggcga acacgtccgg cggccggtct
      421 ccggggtcgt catggagaaa aggcccgaca ccggtgaacg ggttcttgaa ggtggagatc
      481 gatccggctg gcgccgttcc ctcgtagtcc ttgccccgaa tcgtcacacc gagccgatgt
      541 cccgcgggca ccacgatcga agtcggccag atctcgatgt cgaggtcgta ggccacaccc
      601 gggatcagcg cttcgcgatc ggtgtgctgg tggtaggggc gccattctgt ggagcggtcc
      661 gggtcggttc tccgatgcga ggcgcgcagc catccgtggg cgagtggaga gtgtgggtcg
      721 agcgcaccct gaaatgtcac ttcatcgccg gcggggtcga acagacgcag cacgatgaac
      781 aggtcagcgt cctcggtgga agaggagatg cgaagccggg ccgaggacgg accggtgatc
      841 tcgacttcgg tcgggtaggg ctcggtgagg aaggtcagcc cgtcgcccat ggccgggaac
      901 gagacctgcc actccccgtc gacaggctcg ttccccagtc ccatgtcagg gtcgagatac
      961 agtctggtcc acttggtctc gggcaacggc caggagaccg cggtccttgg ctcgaaatgg
     1021 tccacatgac ggacattgag catcaccggg gggcgctggt cccagccgtt gtcctcgtct
     1081 ttcaggaagt gatcgaagaa ctcgagctgg agcagacgcc cgtaatccgt gtagaagtga
     1141 gtccagtgct cgagaccgtg cagctcgagc cacttctgtg tcgatcccgc ccgcatgaaa
     1201 ccctcgacgt ttccccgcag atgaagaccg tggccacccc agttccccgc gctgagcagt
     1261 ggggtcgtga tctgctcgaa gaccgccgat cggtcgcggt ggtactggtc gtcgaacgga
     1321 tgtgaggcga tggcggcacc gagatcgacc cggttcccgg ccaggtcttc gtcagagaga
     1381 tcctcgtcgc cacacacctg caacccgttg atcgaggagg cagggccgcg ggtgcccagc
     1441 ccgtgctgga ccgtcttgac ctgcttgtca taccagttgg cgaagaaggt gcagatgatc
     1501 cctccgtggt gggtcatgtc ccggtagtgg tcggacgctc cttcccagac gcacatcgcg
     1561 gccaggctcg ggggacggag cgacgccgcc tgccactggt tgatcccgta gtaggagacc
     1621 ccgttgagcc cgatcctgcc ggtcgaccat ggttgggccg ccgcccactc gatgctctcg
     1681 tagaggtccc gggtctcgcg tggcgagaaa tgatctatga acccggggga gcggcccgcg
     1741 ccccgggagt cgatgcggag gcaaacgtag ccttcgggga cccacttctc ggggtcgacc
     1801 acctcccagc tctggtactt gttcgaggag cccgcggaga catcgggatg gcctccgacc
     1861 atggcgttcc actgatccgg gtagccgacc tggaagggga gccccttggc gtagggccca
     1921 tagctcacga tgacagggtg actcccggtg tccggtcgat acacgtcggc ccgcaacacc
     1981 agaccgtcgc tcatcgggat gggcacgtcc cagtcggtga tcatcggcgg gacggggtgg
     2041 cggcagtcag gtcgaagagg gacatcgttt tgcaggatat aggatatagc gtccccgtgg
     2101 gcgcggtccg tgaggtcctg tttggggggt cgg
//
LOCUS       DATITW010000149         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_367825, whole genome shotgun sequence.
ACCESSION   DATITW010000149 DATITW010000000
VERSION     DATITW010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367825"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..498)
                     /locus_tag="VLS86_02090"
     CDS             complement(73..498)
                     /locus_tag="VLS86_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003939438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HSO49312.1"
                     /translation="MDYLVVEFPAGASNFTGEMSEELLRLVESGIIRVIDVLILTKAD
                     DGSVDAMELSDVEGLGDLATLEAALAELLAAEDVEPLAAAMDPGSTAGVLIWENLWAA
                     PFAYAARLSGGQLIANGRIPIQAIIASIEADDDTTTEGD"
     gene            905..>2350
                     /locus_tag="VLS86_02095"
     CDS             905..>2350
                     /locus_tag="VLS86_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49313.1"
                     /translation="MRSLAVPGASYFVATAGQPRVRRPRDVLTAIVGLLLVFWAVNAI
                     DQPPSWELSLTELVKSSPPWLQTLLEGAYSFSLIYSLVLFGALLFGGRERRTALRDVS
                     IVAAGAAALVILLSFLINDAWPYVLPEIDLEDPVPRFPVMRVALVTAILIVASPYVTR
                     PLRRFGWLAIVTTAFASISLGYATPSHTIGSFGIGLFTAGVLLAVAGSPRGYPNPEMV
                     AAALTHLGVPNREIRAAPYQTWGVVRFVAIDNDGEPLEVKVHGRDAFDSQLAAKVWHT
                     LWYRETGPAVSYSRLQAVEHEALMAVVANGVGVNVPRLTAVGAASSEIALISFRESGV
                     ALHDLDPDKLTDDQLVETWSQVRLLHDRSMSHGSLRASAVHVSRDGPMITDLALGSLA
                     ADEADQGNDIVELLFSLTALVGEERAVRSALQGLGHERLVAALPYLQVAAVSPTGRRL
                     VEKPKQLMTSLRAKVGEITESEIPEPVKLRRV"
BASE COUNT          411 a          754 c          720 g          465 t
ORIGIN      
        1 acgacggcgg tcttggccac cgggtggccg ataaccccga ctcttcctac tcgacctggt
       61 ctgaggggca tgtcagtcgc cttcggtagt ggtgtcgtca tcggcctcga tcgaggcgat
      121 gatcgcctgg atgggaatcc gtccgttggc gatgagctgc ccgccggaaa gccgcgcggc
      181 gtacgcgaat ggcgcggccc aaaggttctc ccagatgagc acgcccgcag tgctccctgg
      241 gtccatggcg gcagccaggg gctcgacgtc ctccgcggct aaaagctctg ccagcgcggc
      301 ctctagagtg gccaggtctc ccaggccttc gacatcggag agttccatcg catctacgga
      361 accgtcatcg gccttggtga ggatcagcac atctatcacg cggatgatcc cgctttcgac
      421 caacctcagc agctcctcgg acatctcgcc ggtgaaattg ctggcaccgg ctggaaattc
      481 tacaaccagg taatccacag gcccaagctc atccagcgat ctatcggtca ttagtagtcc
      541 gttcgtttgg ggcgatacgt aatggcgcac gtcggcgtcc gggttcggcc acacgatgac
      601 ggtttgaagg atagcgctgg gatggctacg acgccctgtc catcatcaag tcacccgact
      661 accgcgtccc cctcgtcgcg ggtgactcgc tgcgctgggt cccgtaccta ccacgtagct
      721 ttgtagggga gctcgacgta gcgtcgcctt ggatttcgcc gagacccaga agaatccgcc
      781 tgagacctga aatcatgtag gcgccgggat atctcccccc gatcaggcga tgctcgaaag
      841 ggcaccaggg gtagcgtgca gatcgacgat catcacataa ggtgactgga ctcgagagga
      901 tctgatgagg tcgctagctg tccctggggc ctcctatttc gtggccacgg cgggacaacc
      961 aagggtgcgc cggccgaggg acgtcctcac cgcaatcgtc gggctgttgt tggtgttttg
     1021 ggccgtgaac gcgatcgacc agccgccgag ctgggagcta tccctcactg agctcgtgaa
     1081 atcgagcccg ccgtggctgc aaacgctgct ggaaggcgcc tactcgttca gcctgatcta
     1141 ttcattggtc ctcttcggtg ctctcttgtt cggtgggcgc gaacgacgta cggccctgag
     1201 ggatgtgtca atagtggcgg ctggggccgc cgccctcgtg atcctgctct cgttcctgat
     1261 caatgacgcc tggccctatg tcctgcccga gatcgatctc gaggatcctg tgcctcgctt
     1321 ccccgtgatg agggtggcgc tggtcaccgc gatcctcatc gtcgccagcc cgtatgtcac
     1381 ccggcccctc cgccgcttcg gctggctggc gattgtcacc acggcctttg cctccatcag
     1441 tctcgggtac gcaaccccgt cgcacacaat tggctcgttt ggaatcgggc tattcaccgc
     1501 aggggtcttg ctagctgtcg ccgggtcacc tcgcggctat ccaaacccag agatggtggc
     1561 cgccgccctg acccatctcg gcgtcccgaa tcgagagatc cgggccgccc cgtaccaaac
     1621 ttggggtgtc gtccgctttg tagcgatcga caacgatggc gagcccttgg aagtcaaagt
     1681 gcatggtcgc gatgccttcg actcccaact tgcggccaag gtttggcaca cgctctggta
     1741 ccgcgagacc ggcccagcag tcagctattc ccggcttcag gcggttgagc acgaggcgct
     1801 gatggccgtc gtggccaatg gggtcggtgt gaacgtaccc cggctcaccg ccgtgggcgc
     1861 cgcctcttct gagatcgcgt tgatctcctt ccgagaatcg ggggtcgccc tccacgacct
     1921 cgacccggac aaactcaccg acgatcagct cgtcgaaaca tggtcacagg tcaggctcct
     1981 tcacgaccgg tccatgagcc acggttcgct tcgcgcgtcg gcggtacacg tgagccggga
     2041 tggtcccatg atcaccgacc tcgctctggg atccctggcc gctgatgaag ccgaccaggg
     2101 gaacgacatc gtggagctgc tattcagcct gaccgcttta gtcggagagg agagagcggt
     2161 tcgcagcgcc ctccagggac tcggacacga gcggttggtc gccgccttgc cctacctgca
     2221 agtggcagcc gtgagcccga caggccgccg cctggttgag aagccaaagc agttgatgac
     2281 gtccctgcgc gccaaggtgg gcgaaatcac ggaatcagaa ataccggagc ccgtcaagtt
     2341 gcgccgagtg
//
LOCUS       DATITW010000150         1591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_328048, whole genome shotgun sequence.
ACCESSION   DATITW010000150 DATITW010000000
VERSION     DATITW010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328048"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1359
                     /locus_tag="VLS86_02100"
     CDS             73..1359
                     /locus_tag="VLS86_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HSO49314.1"
                     /translation="MAPFAQESGKSAIGDPPERPPYRSGLSPIDVVGPGRHHSPVSQR
                     RPFEFYRRRSVRMIAGVAGGLADELGVNDAFVRGAFVSLTMVWGLGVLIYFGLWLTAI
                     DRVDDVEANPAEPQQVLGLGFAFVGLMLLLGVVGWWPSNALVLTVSALSFGLAALADR
                     NMTNSVTAFFDPNATQPGRWRLLVGVGLLIGGLAMLSTGVGPLLEVGPVLLAVALTVL
                     GILIAFGPWVTRLARDLGAERRERIRQEERAELAAHLHDSVLQTLALIQRTDDPARMS
                     ILARHQEGELREWLYGNAPVDGSDLVSTALKGAATRIETDHQVPIDVVTVGDHPVDES
                     SRALVSAASEALVNAAKHSGSDRLSLFFEAEESQLSVFVTDHGKGFDRSAVPKDRKGI
                     TQSIMARVERAGGKVEINSEPGEGTEVILRMPVRVE"
     gene            1356..>1591
                     /locus_tag="VLS86_02105"
     CDS             1356..>1591
                     /locus_tag="VLS86_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSO49315.1"
                     /translation="MSIKVYLVDDHRLFLAGVKAELEEEFDIVGSAYDVDTAIEEIRG
                     LNPTVVLIDVHMPDGGGIAVVEGVGQTHPDVKFLA"
BASE COUNT          241 a          486 c          535 g          329 t
ORIGIN      
        1 ttgggtcgga tcagctcctt cggcgcaacc accctgggct ctagggcctc ggtagtcgtg
       61 tcgttttgtt ccatggcccc atttgcccag gaatcaggca aatctgcaat cggggacccc
      121 cctgagagac ccccgtatcg ctcagggttg tccccgattg atgttgtggg gccgggccgc
      181 catcattctc ctgtgagtca acgccgcccg ttcgagtttt accgtcgccg gtccgttcgg
      241 atgatcgccg gggtcgccgg ggggctggcc gatgagctcg gggtcaacga cgcattcgtc
      301 cggggtgcct tcgtcagttt gaccatggtg tggggtctcg gtgtcctcat ctatttcggt
      361 ctgtggctga cggcgatcga ccgggtggat gacgtcgagg cgaatcccgc cgagccacag
      421 caggtgctcg gtctcggctt cgccttcgtc gggttgatgc tgctcctcgg agtcgtggga
      481 tggtggccat cgaatgcgct ggtgctcacc gtctcggcgt tgagcttcgg cttggctgcc
      541 ctcgccgatc gaaacatgac caactcggtc acggcgttct tcgaccccaa cgccacccag
      601 cccgggcgat ggcgcctgtt ggtcggcgtc ggcctgttga tcggcggact ggcgatgctc
      661 tccacgggtg tcggccccct tctcgaggta ggtccggtcc tgttggcggt ggcgctcacc
      721 gttctcggaa tcttgatcgc ctttgggcct tgggtcaccc gtctcgcccg ggatctgggg
      781 gccgaacgaa gggagcggat ccgccaggag gagcgcgccg agttggcggc gcatctccat
      841 gactcggtgc tgcagactct ggccttgatc caacgaaccg atgatccggc ccggatgtcg
      901 atcctcgccc gccaccagga gggcgagttg cgagagtggc tttacgggaa tgcccccgtc
      961 gacggttccg acctggtctc gactgctctc aagggggcag ccacccgcat cgagaccgat
     1021 catcaggttc cgatcgacgt ggtgactgtg ggggaccatc cggttgatga gtcttcccgg
     1081 gcattggtca gcgccgccag cgaggcgctc gtgaatgccg ccaagcactc gggctcggac
     1141 cggctctccc tcttcttcga ggccgaggag tcccagctct cggtgttcgt caccgaccac
     1201 ggcaagggct tcgaccgatc tgcggtgccg aaagaccgca aagggatcac tcagtcgatc
     1261 atggcgcggg tggaacgcgc cgggggcaag gtggagatca acagtgagcc gggggagggc
     1321 accgaggtca ttctccggat gccggtgcgg gtggaatgag catcaaggtc tacctcgtcg
     1381 acgatcaccg gttgttcctg gccggggtca aggccgagct cgaagaggaa ttcgacatcg
     1441 tcggctcggc ctatgacgtc gacactgcca tcgaggagat tcgtggtctc aacccgaccg
     1501 tggtgctgat cgatgtccac atgcccgatg gcggtggaat tgcggtggtg gaaggagtcg
     1561 gccagaccca ccctgacgtg aagtttctcg c
//
LOCUS       DATITW010000151         1548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_608314, whole genome shotgun sequence.
ACCESSION   DATITW010000151 DATITW010000000
VERSION     DATITW010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608314"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VLS86_02110"
     CDS             <1..231
                     /locus_tag="VLS86_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSO49316.1"
                     /translation="EGALANSLSEAMRRLREMDLFKPPGVAETLDWAESLRLLGAGEL
                     SPKTIESTLGVVLKYKDDIDMVRAAGVDSLVG"
     gene            228..1346
                     /locus_tag="VLS86_02115"
     CDS             228..1346
                     /locus_tag="VLS86_02115"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSO49317.1"
                     /translation="MKRAGLIDQLSVFSGALRNRGLTVTSDQTADMARALTLIDLTRR
                     SQAEASLRSLAVTDPAQIPAFAEEFERFFDRIRPPTLVDRDPDTPSSSGASYTLQGTP
                     ATSEAEVSQHHGASAVERISARDFADLDEDDIQEARRLVMTMLWHPADYKTRRWSPSN
                     YGNRPDLRRTLHNAVGPTGDMMFLSWKQRRRKERPLIIIADISGSMEKYADIFLVFAH
                     AAQRRVREVEIFTFSTRLSRITEDMRHRDTKVALAGVASRVTDWAGGTQIGEALAEWN
                     RRWSRRLARGGPVVLILSDGWDCGDPEILSTEMGRLARSVHQVIWLNPLAARTSYQPL
                     TRGMQAVLPHVDQLLPAASVNDFKGVVRVLDSITRGKR"
     gene            1343..>1548
                     /locus_tag="VLS86_02120"
     CDS             1343..>1548
                     /locus_tag="VLS86_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006115363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HSO49318.1"
                     /translation="MRHQIEVRNRWAGEGKKVAVATVIRVHGSAPRPEGSKFLVSDAG
                     DMEGSVSGGCVENDVFLHAQEVLET"
BASE COUNT          270 a          522 c          510 g          246 t
ORIGIN      
        1 gagggggcct tggccaactc gctcagcgag gcgatgcggc ggctccggga gatggacctg
       61 ttcaagccgc cgggtgtggc cgagacactc gactgggcag agtcgttgcg tttgctcggg
      121 gccggggagc tgtcgccgaa gaccatcgag agcaccctcg gggtcgtgct caagtacaag
      181 gacgacatcg acatggtgcg cgccgccgga gtcgacagcc tggtgggatg aagcgcgccg
      241 ggctcatcga tcagctgagc gtgttctccg gcgccctgcg caaccggggg ctgaccgtga
      301 cttcagacca gaccgccgac atggcccggg cgctgaccct gatcgacctc acccgacgtt
      361 cgcaggccga ggcatcgctc aggtcgctcg cggtgacaga tcctgcccag atcccggcct
      421 tcgccgagga gttcgagagg ttcttcgatc ggatccgccc gccgacgctc gtcgaccggg
      481 acccggacac accaagctct tcgggcgcca gctacacgtt gcagggcacg ccggccacct
      541 cggaggccga ggtgagccag catcatggcg cttcggcagt ggagcggatc tccgctcgcg
      601 actttgccga tctggacgaa gacgacatcc aggaggcgcg gcgtctggtc atgaccatgc
      661 tctggcaccc ggccgactac aagacccgtc gctggtcgcc gtcgaactac ggcaatcgcc
      721 ccgacctgcg ccgcaccctg cacaacgcgg tggggccgac gggggacatg atgttcctca
      781 gctggaagca gcgacggagg aaagaacggc ccctgatcat catcgccgac atctcgggct
      841 ccatggagaa gtacgccgac atcttcctgg tctttgccca cgccgctcag cgtcgggttc
      901 gcgaggtcga gatcttcacc ttctccaccc ggctcagccg gataaccgag gacatgcgcc
      961 atcgcgacac caaggtcgcc ctggccggtg tcgccagccg ggtcaccgac tgggcgggcg
     1021 gcacccagat cggagaggcg ctcgccgaat ggaatcgacg ctggagccga cgtctcgccc
     1081 gcggcgggcc ggtggtgctg attctctccg acggttggga ctgcggcgac ccggagatcc
     1141 tgtccaccga gatgggccgt ctcgcacgtt cggttcacca ggtgatctgg ctcaatcccc
     1201 tcgccgcccg aacctcatat cagcctctca cccgggggat gcaggccgtc ctgccccacg
     1261 tcgatcagtt gctccctgcg gctagcgtca acgacttcaa gggagtggtc agagtgctcg
     1321 actccatcac cagaggcaag agatgagaca ccagatagag gttcgcaacc gctgggccgg
     1381 tgaaggcaag aaggtggccg tcgccaccgt gatccgggtc catggcagcg ccccccggcc
     1441 cgagggatcc aaattcctcg tctccgacgc cggggacatg gagggctcgg tcagcggggg
     1501 ctgtgtcgag aacgacgtct tcctccacgc ccaggaggtc ctcgagac
//
LOCUS       DATITW010000152         1540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_127749, whole genome shotgun sequence.
ACCESSION   DATITW010000152 DATITW010000000
VERSION     DATITW010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127749"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VLS86_02125"
     CDS             complement(<1..644)
                     /locus_tag="VLS86_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005447755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HSO49319.1"
                     /translation="MGSGIAEVCARAGSTVTIHEIDGETAAAALARVERSLDRALEKG
                     KLDAPERDAILGRITVSTDLGEQADRQLVIEAATEDEGLKRKVFADLDSIVATDTILA
                     SNTSSIPITRLASATSRPDPVVGLHFFNPVPVMALVELVTTVMSSAEVESRAQEYAES
                     LGKTVIRAKDRAGFIVNMLLVPYLLGAIRMYEAGVASREDIDNGMRLGANHPMGP"
     gene            814..>1540
                     /locus_tag="VLS86_02130"
     CDS             814..>1540
                     /locus_tag="VLS86_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSO49320.1"
                     /translation="MTYDHKAIEAKWQQRWETEGVFRAAEDEAMPKFYNLCMYPYPSG
                     ALHQGHVRNYTFGDLLTRYKTMRGFNVLSPMGWDSFGLPAENAAIESGVHPRENTEAQ
                     IVTMTRQIKQLGAMYDWDREIASHHPDYYRWDQWIFLQLLDRGLAYKKEAPVNWCPRD
                     QTVLANEQVVDGACERCGTIVEKKNLAQWFFKITDYADRLLDDLDTLDEWPDRVRTMQ
                     RNWIGRSEGVRFTLEVDGLDESFA"
BASE COUNT          287 a          502 c          483 g          268 t
ORIGIN      
        1 gggcccatcg ggtggttcgc cccgagacgc atcccgttgt cgatgtcctc ccgggaggcc
       61 acccctgcct cgtacatccg gatcgctccc agcaggtagg ggaccaggag catgttgacg
      121 atgaagccgg cccggtcctt ggcccggatc acggtcttgc ccaacgactc ggcgtactcc
      181 tgggctctgc tctcgacctc ggccgaggac atgacagtcg tcaccagttc cactagagcc
      241 atcaccggga ccgggttgaa gaagtgcagc ccgacgacag ggtcgggtct cgatgttgcc
      301 gaggcgaggc gggtgatcgg gatcgaggat gtgttggagg cgaggatggt gtcggtggcg
      361 acgatcgaat ccaggtcggc aaagaccttc ctcttcagcc cttcgtcctc cgtggccgcc
      421 tcgatcacca gttgacggtc cgcctgttcg ccgagatcgg tcgagaccgt aatccgcccc
      481 aagatcgcgt cgcgctccgg ggcatcgagc ttgcccttct ccagggcccg gtccagtgac
      541 ctctcgacac gggccagagc tgcggccgcc gtttcgccgt cgatctcgtg gatcgtgacc
      601 gtgctgcccg cgcgggcaca gacctcggcg attcccgagc ccatgagccc gccgccgatg
      661 acgccgacac gttcaatcgc caaagcagca ccacccttct tctgtaattg ggatcgacag
      721 gatcgtatat gacccctgac cgtcccaatt acggggaggg ggaggaccgg gtgggacacg
      781 cacctcgccc gacgcattta ccctgagggg ccgatgacct acgaccacaa ggcgatagag
      841 gccaaatggc agcagaggtg ggagacggag ggggtattcc gcgccgccga ggacgaggcc
      901 atgcccaagt tctacaacct gtgcatgtac ccctacccgt cgggggcgct tcaccagggc
      961 cacgtgagga actacacctt tggcgacctg ctcacccggt acaagaccat gcgcgggttc
     1021 aacgtgctct ccccgatggg ctgggacagt ttcgggttgc cggctgagaa tgccgccatc
     1081 gagtccggtg tccaccccag ggagaacacc gaggcccaga tcgtcaccat gacccgacag
     1141 atcaaacagc tcggcgccat gtacgactgg gaccgcgaga tcgcctcgca tcatcccgat
     1201 tactaccgct gggaccagtg gatcttcctc cagctgctcg accgtggact ggcatacaag
     1261 aaggaggcac cggtcaactg gtgtccccgt gatcagaccg tgctcgccaa cgagcaggtc
     1321 gtggacggcg cttgtgagcg ctgcggcacc atcgtcgaga agaagaacct ggcgcagtgg
     1381 ttcttcaaga tcaccgacta cgccgaccgg ctactcgacg acctcgacac cctcgacgag
     1441 tggcccgatc gggttcggac catgcagcgc aactggatcg ggcggtccga gggcgtcagg
     1501 ttcaccctcg aggtcgatgg tctcgatgag agctttgctg
//
LOCUS       DATITW010000153         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_448250, whole genome shotgun sequence.
ACCESSION   DATITW010000153 DATITW010000000
VERSION     DATITW010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448250"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..526)
                     /locus_tag="VLS86_02135"
     CDS             complement(68..526)
                     /locus_tag="VLS86_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49321.1"
                     /translation="MDRAPDSLGPTVDVALLADAVQAVRGKLFILGGGWDTLWVRSFP
                     ARHPSLAIGLRLRVPSSWGEEMLKLSVELQDADGAPLLPHPLAHNIKLPAHPQPPSAT
                     DFGLIRSFTFNNLLFGGEGSYSFVISVDEEPVSRLRFSVRARPDDPPPGL"
     gene            640..1962
                     /locus_tag="VLS86_02140"
                     /pseudo
     CDS             640..1962
                     /locus_tag="VLS86_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
BASE COUNT          411 a          680 c          670 g          364 t
ORIGIN      
        1 ggctttttct tatgccaacc ccgcgcttac cgtgacaact gcgcgcgcag agcgcgaggt
       61 ggtcgattca gagaccgggt ggtgggtcgt caggtcgggc ccgcaccgag aaccggagcc
      121 gggagacagg ctcttcgtcg acggagatca cgaacgagta ggagccctcg cccccgaaca
      181 ggaggttgtt gaaggtgaag gacctgatga gcccgaagtc ggtggccgac ggaggttggg
      241 ggtgggccgg gagcttgatg ttgtgggcga ggggatgtgg gagcaggggc gcgccatcgg
      301 cgtcttgaag ctccaccgac agcttgagca tctcctctcc ccaggaggac ggcacccgca
      361 gccggagacc tatcgccaga ctcgggtgtc tggcaggaaa cgatcgcacc cacagcgtgt
      421 cccagccacc gccgaggatg aacaacttgc cccgaaccgc ctggactgcg tccgcgagca
      481 gcgcgacatc gaccgtcggg ccaagtgagt ccggggcgcg atccatgccg caacactacg
      541 gccactccac accccggcgc gtcccgcagc ttcgcctctc gccgccccgg tgcagggcgg
      601 cgatggccca tgccctatcg tttgtcgcca ggaggtttca tgtctgacaa tcagtctccg
      661 gctccggcca ccgggaagct cggggtgctc accccgggga tgggcgccgt cgcgacaacc
      721 ttcatctccg gtgtcctcgc ctcgcgcaag ggctacgccg tcccctacgg atcgttgagc
      781 cagctgtccc atatccgtct gggcaagcgg accgatgagc ggaacccgct gatcagggag
      841 ttcgtcccgt tggccggtct cgacgacctc gagttcgggg gttgggaccc gtattcagac
      901 gatgcctaca catcggcgat gaaggccggg gtgctcgacc cgaggcacat cgaagggatc
      961 ggcgacgagc tgaggtcgat caagccttac cccgcggtgt tctcgcaccg gtggatccga
     1021 aacctcgacg gcgtcgacca ggtcaaggag ggagtcacgc acatggacct cgccgagagc
     1081 ctgatggagg acatcgatcg ctttcgctct gacaggcagg tggaccggct ggtcatggtc
     1141 tggtgcgggt ccaccgaggc ctatgaagag ccttcccagg tacatgccga gcttcagacc
     1201 ttcgagcaag gcttgaagga caacgatccg gcgatcactc cctcccagat ctatgcctac
     1261 gccgccctga agatgaaggt gcccttcgcc aacggagctc ccaatctgac cgtcgatatc
     1321 ccggccatgg tcgagttggc ggtccaggag ggtgtcccca ttgccggcaa ggacttccag
     1381 accggccaga ccctgatgaa gacgatcgtg gcccctggtc tcaaggcgcg gatgctcggc
     1441 gtgaagggct ggttctccac caacatcctc gggaaccgcg acggtgaggt gctcgatgac
     1501 cccgacagct tcaggtcgaa ggaggtctcc aagctgggag tgctggagca gatcctccag
     1561 cccgacgtct accccgagct gtacggcgac ctctatcaca aagtccggat caactactac
     1621 ccgcctcttg gtgacgacaa ataaggatgg gacaacatcg acatcttcgg ttggatgggt
     1681 taccccatgc agatcaagat cgacttcctg tgcaaggact cgatactcgc cgctcccatc
     1741 gtgctcgacc tcgccctgtt catggacctc gcggcccgaa ccgggaggaa gggagtccag
     1801 gagtggctct ccttctactt caagagccct caggtggccg aaggcctcta cgccgagaac
     1861 gacttgttca tccagctcac caagctgaag aacaacctcc ggcacatgat gggtgaagac
     1921 ctgattactc acctcggcac cgagtactac caagaggcat gaccaccgag gggatcactg
     1981 tcggccaatg gctgatcccc gagtcggaga tcgacgagcg gttcgacacc tcgggtggcc
     2041 ccgggggcca gcacgccaac cggaatgaga ccgcggtgac tctgcgcttc gacgtggcgg
     2101 cgtcgtcgct cccccaggag atccg
//
LOCUS       DATITW010000154         1604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_768749, whole genome shotgun sequence.
ACCESSION   DATITW010000154 DATITW010000000
VERSION     DATITW010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768749"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1604
                     /locus_tag="VLS86_02145"
     CDS             <1..>1604
                     /locus_tag="VLS86_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HSO49322.1"
                     /translation="IFKFGSGAGSNFSHIRGENERLSGGGKSSGVMSFLKIGDRAAGA
                     IKSGGTTRRAAKMVILDVDHPDIEKFIDWKKVEEDKARVLINHGGLPADFNGDAYATV
                     SGQNSNNSVRITNEFVQTVIDDGDWSLFNRTDGSVRKTVKARDLWNKIAEAAWACADP
                     GLQFDTTINEWHTSPAGGRIRASNPCFTGEMRVATDKGLIPFKSLVTRVAEGESFQIY
                     THDATNPDVPTETIDLTTPTQVMVTGVNQLHRREFSDGRVLRCTPNHRLWTANREWVR
                     ADELAESDQIRLLDQEIDFGMASWAMPVSTEAATYQQRASSRKYAVNLPEKWTEDFAH
                     YLGWLVGDGSVTSKAAVTIYGSDEEQQTTMWLHRELLAELNGGVAPRPVEMANGTYQL
                     RVTRNAIRKFFEALGLSPVKADDKRVPWSIFEAPKPIVAAFLRGLYDADGCVRYGDTT
                     RYAGLASASKELLRDVQQLLDTFGVHGTIYSSKRKESPTFEYTTKDGEHRTYRGRSEI
                     NDLRVMGSDLERFFHAIGFGLANKDD"
BASE COUNT          341 a          498 c          491 g          274 t
ORIGIN      
        1 atcttcaagt tcgggtcagg cgcggggtcc aacttcagcc acatccgcgg cgagaacgag
       61 cggctctccg gcggtggcaa gtcgtcaggt gtcatgtcct tcctcaagat cggtgaccgg
      121 gcggcgggcg cgatcaagag cggagggacc acgagacgcg ccgccaagat ggtgatcctc
      181 gacgtcgacc atcccgacat cgagaagttc atcgactgga agaaggtcga ggaggacaag
      241 gcgcgggtcc tgatcaacca tggcggcctc cccgccgact tcaacggtga cgcctacgcc
      301 accgtgtccg ggcagaactc gaacaactcg gttcgaatca ccaacgagtt cgtccagacc
      361 gtgatcgacg acggtgactg gagtctgttc aaccgcactg acggttcggt tcggaagacg
      421 gtcaaggcac gtgacctatg gaacaagatc gccgaggcgg catgggcctg cgccgacccc
      481 ggtctccaat tcgacaccac gatcaacgag tggcacacca gtcccgccgg tggccggatc
      541 cgggcatcca acccatgctt caccggtgag atgagggttg ccaccgacaa agggctcata
      601 ccgttcaaat cactggttac ccgggtcgcc gaaggcgagt cgttccagat ctacacccac
      661 gacgccacca accccgacgt cccaaccgag accattgacc tgaccacccc gacccaggtg
      721 atggtgactg gagtcaatca actccaccgg cgcgagttct ccgatggccg ggtgttgcga
      781 tgcactccca accaccggct gtggactgcg aaccgcgaat gggtgcgagc cgacgagctg
      841 gccgagtccg atcagatccg gcttctggat caggagatcg acttcggcat ggcctcatgg
      901 gcgatgccgg tttcaaccga ggctgcgaca tatcaacagc gcgcctcgag ccgcaagtac
      961 gcggtcaacc tgccagagaa gtggaccgag gactttgctc attatctcgg ctggctggtc
     1021 ggcgacggca gcgtgacatc caaggccgct gtaaccatct acggatcgga cgaagagcag
     1081 cagaccacca tgtggctgca tcgtgaactg ttggccgagc tcaacggtgg ggtcgcgccc
     1141 aggccggtcg agatggcgaa cgggacctac cagctccggg tgacgcgaaa tgcaatccgc
     1201 aagttcttcg aggcccttgg gttgtcaccg gtcaaagccg acgacaagcg ggtgccatgg
     1261 tcgatcttcg aggcgccaaa gccgatcgtg gccgccttcc tccgcgggct ctacgacgcc
     1321 gacggctgcg tccggtatgg cgacaccact cgttatgccg gcttggccag tgcatcgaag
     1381 gaactgttgc gagatgttca gcagcttctg gacacctttg gagtgcacgg cacgatctac
     1441 tcgagcaagc ggaaagaatc cccgaccttc gagtacacga ccaaggatgg cgaacatcgc
     1501 acctatcggg gtcgctccga gatcaacgat ctccgagtca tggggtcgga cttggagagg
     1561 ttcttccacg ccattgggtt tggcctggcc aacaaggacg acat
//
LOCUS       DATITW010000155         1929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_568599, whole genome shotgun sequence.
ACCESSION   DATITW010000155 DATITW010000000
VERSION     DATITW010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568599"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VLS86_02150"
     CDS             <1..694
                     /locus_tag="VLS86_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49323.1"
                     /translation="AAGALAGLIQGFLVGRVGLPSLVVTLAGLIGFRGAARVLLEDRS
                     VGDFPEWFDRLGQQPLIGPIPLALFVFFAGLAVAWVTLERGALGREVYVIGDSADVGR
                     YSGIEVGGLKLRLLVTSGLVAGLAGVLFAARLGSVRGSLGEFFELDIITMVLLGGVSI
                     FGGSGSMLGVVLAIFTVLNIRNGLGLANVDGIVQTGVIGLLLIGSVLIPNLAQRFGSR
                     RVAHEAVAPGPS"
     gene            773..1927
                     /locus_tag="VLS86_02155"
     CDS             773..1927
                     /locus_tag="VLS86_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSO49324.1"
                     /translation="MTAIKRRMRILGPLAALVLVVAACGDDGGDATTTTAGDPTTTVA
                     EPTTTAGEEPTTTTAAPAPEAVEITGSGSITSTFLPKCTNIAVFPQAFEGSEEAATEL
                     GSDPAEFVGPADCGDATGQIEFMSNAVTQGVQAIMVSNNAGDELAPSAEAAAAAGIPV
                     VAWDSPIPSAAGESVFIAQVDFDETGVVMADMALSILGDDGGEFAILSATPEAANQNA
                     WIAAMEEALAADAKYANLVLVDTVYGNDVAEDSVTAALGLVDSHPDLELIMAPTTVGI
                     VAAAQAMTQEGLCDQVKVSGLGLPAEMAEYVESGCAPEFALWSFVDLGYLAYYVAYGI
                     ATEQIEGVEGETFVAGRMGEYTLTADPTRDAGLRVLMGPFSVYNAENPGT"
BASE COUNT          325 a          586 c          655 g          363 t
ORIGIN      
        1 ggctgccggc gctcttgccg gtttgatcca gggttttctg gtgggtcgcg tcggcctgcc
       61 ttcgctggtg gtgactcttg ccggcttgat cggattccgc ggagcggcac gggtcctgct
      121 cgaggatcgg tcggtcggcg acttccccga gtggttcgat cgtctgggtc agcaaccgtt
      181 gatcgggccc attcccttgg ctctctttgt cttctttgcc gggctggcag tcgcttgggt
      241 caccctcgag cgcggtgccc tcggtcgaga ggtgtatgtc atcggggaca gcgctgacgt
      301 gggacgctat tccggtatcg aggtcggcgg cttgaaactc cgcctgttgg tcacgtcagg
      361 actggtggca gggctggccg gcgtcttgtt cgccgctcgg ctcggatcag tgcggggcag
      421 cctcggcgaa ttcttcgaac tcgacatcat caccatggtc ctgctcggtg gagtcagcat
      481 cttcggcgga tcgggctcga tgctgggcgt ggtcctggcc atcttcaccg tgctcaacat
      541 ccgcaacggc ctcgggctgg ccaatgtcga cggcatcgtc caaaccggcg tgatcggtct
      601 gttgctgatc ggatcggtcc tgatcccaaa tctcgcccag agattcggct cgcgccgcgt
      661 ggcgcacgaa gccgtggctc ctgggccgtc ttgacgaacg acagaaaacg ggaggtcatc
      721 gagtcagaga acaaccagca agacaccgga caaacaagag gaggaacgga aagtgacagc
      781 aatcaagcga aggatgcgaa tactggggcc attggcagct ttggtcctgg tggtcgccgc
      841 ctgcggggat gatggcggtg acgccacaac gactaccgca ggcgatccca cgaccaccgt
      901 tgcggaaccg accacgactg caggtgaaga acccaccacc accacggcag ctccagcgcc
      961 ggaagcggtg gagatcaccg gctccgggtc gatcacctcg acattcttgc cgaagtgcac
     1021 caacattgcc gtattcccac aggccttcga gggctccgag gaagcggcga ccgagctcgg
     1081 atccgatcct gcggagttcg tcggcccggc ggactgtggt gacgccaccg gtcagatcga
     1141 gttcatgagc aacgcggtga cccagggcgt ccaggcgatc atggtctcca acaacgccgg
     1201 cgacgagttg gcgccctccg ccgaagccgc ggcggcagcg gggatacctg ttgtggcttg
     1261 ggactctccg attccgtcgg cggcggggga gagcgtgttc atcgcccagg tcgacttcga
     1321 cgagaccggg gtggtgatgg cggacatggc gctgtccatc ctgggtgacg acggcggcga
     1381 attcgccatc ctgtcggcga ccccggaggc ggcgaaccag aatgcctgga tcgcggccat
     1441 ggaggaagct ctggctgcgg acgccaagta cgccaatctg gtcctggtcg acacggtcta
     1501 cggcaacgac gtggccgagg attcggtcac tgccgcgttg ggactggtcg actcgcaccc
     1561 cgacctggag ctgatcatgg ctccgaccac ggtcgggatc gtggcggctg cccaggccat
     1621 gacccaagaa gggctgtgtg accaggtcaa ggtctccggt ctgggtctcc cggccgagat
     1681 ggcggagtac gtcgagagcg gctgcgctcc cgagtttgcg ctctggagct ttgtcgacct
     1741 cggctatctg gcgtactacg tggcttacgg gatcgccacc gagcagatcg agggcgtcga
     1801 gggtgagacc ttcgtggcgg gacggatggg tgagtacaca ctcaccgccg acccgactcg
     1861 tgatgcaggt ctgagggtgc tcatgggccc cttcagcgtt tacaacgcgg agaaccccgg
     1921 cacctgact
//
LOCUS       DATITW010000156         1794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_488390, whole genome shotgun sequence.
ACCESSION   DATITW010000156 DATITW010000000
VERSION     DATITW010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488390"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..489
                     /locus_tag="VLS86_02160"
     CDS             28..489
                     /locus_tag="VLS86_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HSO49325.1"
                     /translation="MDESLAMHHYNQFFFHGTSHWLGLDVHDAGAYRIDGVSRKLEPG
                     MAFTVEPGLYVAPHKAEIELSLLEYDLDEWAQRRILQGRAAAMAKEQEARDGAEKLKH
                     QVPEAFLGIGIRIEDDVVITDQGHENLTSAVPTGLEEIEALCAEESLLPTG"
     gene            complement(486..1148)
                     /locus_tag="VLS86_02165"
     CDS             complement(486..1148)
                     /locus_tag="VLS86_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HSO49326.1"
                     /translation="MERLTLGRMQNPIRGLLHGAAAIAAIFGLFVLLSRAWGNRAAAV
                     GALVFGVAMLAMYTVSTLYHSVPWGAEWKLRLQRLDHSMIFLLVAGTFTPIAIAAVDG
                     AILVLALTLVWTTAIAGIILKVFLPNVKTTFSVTLQMVMGWSALVLMPWVLSRLGLGA
                     VVLIALGGLCYTVGMVIFTSKRPKLFPRTFSYHELFHVLVIGGTTFHFLAVLWYAIPA
                     TG"
     gene            1252..>1794
                     /locus_tag="VLS86_02170"
     CDS             1252..>1794
                     /locus_tag="VLS86_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HSO49327.1"
                     /translation="MALGLLYRIYERNLLHGLDKDRLPRHIGIVLDGHRRHAREERLP
                     SYADSYRVGMRRFEEFLGWAHELEIPAVTAWVLSKENLIRPDEELAPYFEVLIELFER
                     LPALCEKYDMAIKFIGSLDLLPDQLVSTAKSAEEAHPRGSRRLNVAMGYGGRQEIVDA
                     AKDLVSSLLSDGVPPEEVASY"
BASE COUNT          358 a          572 c          568 g          296 t
ORIGIN      
        1 gtcgagttgg gcctgctccc gaagaccgtg gatgagagcc tggcgatgca ccactacaac
       61 cagttcttct tccatggaac cagtcactgg ctcggtctcg atgtccacga tgcaggcgcc
      121 tatcgaatcg atggagtgtc acgcaagctg gagcccggga tggccttcac cgtggaaccc
      181 ggcctctacg tcgcgccgca caaggccgag atcgagctca gcctcctcga gtacgacctc
      241 gacgaatggg cccagcgcag gatcctccaa ggccgggcgg cggccatggc caaggagcag
      301 gaggcgcggg acggtgcgga gaagctcaaa caccaggtac ccgaggcctt tctcggaatc
      361 gggatccgga tcgaggacga cgtcgtgatc accgaccagg ggcatgagaa cctgacgtcg
      421 gcagttccca cgggcctgga ggaaatcgag gcgctctgcg ccgaggagtc gttgctcccg
      481 accggttagc cggtggccgg gatcgcgtac cagagaaccg cgaggaaatg gaacgtggtc
      541 cctccgatca ccagcacgtg gaacagctcg tgataggaga acgtcctggg aaacagcttg
      601 ggtcgcttcg aggtgaagat caccatccca accgtgtaac agaggccccc cagcgcgatc
      661 agcaccaccg cgcccaggcc gagccggctc agcacccagg gcatcaagac caacgccgac
      721 catcccatca ccatctgcaa ggtgaccgag aaggtagttt tcacgttggg gaggaacacc
      781 ttgaggatga tcccggctat ggcggtggtc cataccaggg tcagtgccag gaccaggatc
      841 gccccgtcga cggcagcgat ggcgatcggg gtgaaggtcc cggccaccag gagaaagatc
      901 atggaatggt cgagtcgctg caggcggagt ttccattccg caccccacgg gaccgagtga
      961 tagagggtcg agaccgtgta catcgccagc atcgccactc cgaacaccag cgcccctacg
     1021 gcggctgccc gattgcccca cgcccggctg aggagcacga acaacccgaa gatggcggct
     1081 atggcggcgg cgccatgaag aagcccccga atcgggttct gcatccggcc gagggtgagt
     1141 ctttccatct gctcccagga tatttgggcc caacgccaag cggtaagccg acgtcggatg
     1201 ccgaccgcgc tggcgaggcc cctcggcgcc ggtagcatca cagggttcgc catggctctc
     1261 ggccttctct accgcatcta cgagcgaaac ctgctccacg gtctcgacaa ggaccgtttg
     1321 ccccggcaca tcggcatcgt tctcgacggt catcgccgcc acgctcggga ggagcggctt
     1381 ccctcctacg ccgattccta tcgggtgggg atgcgtcgtt tcgaggagtt ccttggatgg
     1441 gcccacgaat tggagattcc ggcggtcacc gcctgggtcc tgtcgaagga aaacctgatc
     1501 cgtcccgatg aagagctggc cccctatttc gaggtgctca tcgagctatt cgagcggctc
     1561 ccggcgctgt gcgagaaata tgacatggcc atcaagttca tcgggagcct cgacctgctc
     1621 ccggaccagt tggtgtcgac ggcaaagagc gccgaggagg cgcatcccag agggtcccgg
     1681 cgactcaacg tcgccatggg ttatggcggt cgtcaagaga tcgtcgacgc cgccaaggat
     1741 ctggtctcca gcctcctctc cgacggtgtg cctcccgagg aggtggcgtc ctac
//
LOCUS       DATITW010000157         1992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_87962, whole genome shotgun sequence.
ACCESSION   DATITW010000157 DATITW010000000
VERSION     DATITW010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87962"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..755
                     /gene="extP"
                     /locus_tag="VLS86_02175"
     CDS             6..755
                     /gene="extP"
                     /locus_tag="VLS86_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HSO49328.1"
                     /translation="MKYRLNKTVDSVQGSQAWASNFRPGSIFRKGYTDSPRNRSYVVM
                     NSVLYHLHPVKVKRHGVRLSYTLCLGGLSFFLFILLTITGIFLMFYYRPTSPQAYFDI
                     QALSTDVAFGQLVRNMHRWGAHLMVLSVFLHMSRVFYHGAYKPPREFNWVVGVVLLFL
                     TLLLSFSGYLLPWDQLALWAVTVGTNMAGFVPVVGAQVKFALLAGVEVSAATLLRFYV
                     LHVLALPFILVIFLAVHFWRVRKDGGISGPL"
     gene            767..1855
                     /locus_tag="VLS86_02180"
     CDS             767..1855
                     /locus_tag="VLS86_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49329.1"
                     /translation="MTEIPESLLRRSAEAKAKALGLPVEQVLAEMKGEAPPTPPPAPE
                     PEPEEAVVEDAVEEVPPASATPVEEPVVEVVSEPVVAAEAPHEAPVEDSTPPEPEPDD
                     TAVEASEESGSDEPEAPAPAIEEPETPAPAIEESAAAAVEAEEAPAAVATMVEVPPPA
                     VEGNGAGNGKVASTVSPRPVVPPTGTPEGVRTQRLLTVVKARAIQTVKAEPTDKVNTW
                     PHLMLMEFGALLAITGFLVILAVIIHSPLLEPANFNSTPNPSKAPWYFLGLQELLSYF
                     DPQIAGVTVPTITGMIGFMMVPYVDKNPSHRPSDRKFAIALYSTFLAGAATLTIIGVL
                     FRGRGFNFSYPWKDGVFFDDLKDWVQFE"
BASE COUNT          321 a          680 c          627 g          364 t
ORIGIN      
        1 cgacgctgaa gtaccgtctg aacaagacgg tcgactcggt gcagggcagc caggcctggg
       61 ccagcaactt ccggccgggc tcgatcttcc ggaagggcta caccgacagc ccgaggaacc
      121 ggtcgtacgt ggtgatgaac agcgtgctct accacctcca cccggtgaag gtgaagcggc
      181 acggggtgcg gctctcctac accctctgcc tcggtgggct cagcttcttc ctgttcatcc
      241 tgctcaccat caccggcatc ttcctgatgt tctactaccg gccgacctcg ccgcaggcct
      301 acttcgacat ccaggcgctc tccaccgatg tcgccttcgg ccagctcgtc cgcaacatgc
      361 accggtgggg cgcccatctc atggtgttgt cggtctttct ccacatgagc cgggtcttct
      421 atcacggcgc ctacaagcca cctcgcgagt tcaactgggt ggtcggagtg gtgctgttgt
      481 tcctcaccct gctcctctcc ttctccggct atctgttgcc ctgggaccag ctggcgctct
      541 gggctgtcac ggtcgggacc aacatggccg ggttcgtgcc ggtggtgggg gcgcaggtga
      601 agttcgcgct gctcgccggg gtggaggtct cggcggccac cttgctccgg ttctacgtgc
      661 tccacgtgct ggccttgccc ttcatcctgg tgatcttcct cgccgtccat ttctggaggg
      721 tgcgcaagga cggcgggatc tcaggtcccc tctaaggagt attgaaatga ctgagatccc
      781 agagagcctc ctcagacggt cggcagaagc caaggccaag gcgctcggtc tgcccgtgga
      841 gcaagtcctc gccgagatga aaggcgaggc cccgcccact ccacccccgg cgccggaacc
      901 cgagccggag gaggcggtcg tcgaggacgc ggtcgaggag gttccacctg cctcggcgac
      961 cccggtcgag gaaccggtgg tggaggtcgt atccgagccg gtggtggcag cggaagcgcc
     1021 acacgaggct ccggtagagg attccactcc ccccgagccc gagcccgacg acaccgcggt
     1081 ggaggcgtcc gaggaatcgg gctccgacga gcccgaggcg cctgccccgg cgatcgagga
     1141 gcccgagacg ccggcccctg cgatcgagga gtctgccgcg gcggcagtgg aggccgaaga
     1201 ggctccggcc gcggtcgcca ccatggtcga ggtgccgcca ccggcggtcg aaggaaacgg
     1261 cgcaggaaac gggaaggtgg cttccaccgt cagcccccgg cccgtggtgc cccccacggg
     1321 gaccccggaa ggggtgcgga cccaacgact cctcaccgtc gtcaaggccc gtgcgattca
     1381 gaccgtcaag gccgagccca ccgacaaggt gaacacctgg ccgcatctga tgctgatgga
     1441 attcggagcc ttgctcgcca tcaccggttt cctggtcatc ctcgcggtga tcatccactc
     1501 gccgttgttg gagcctgcga acttcaactc cactccgaac ccttccaagg cgccgtggta
     1561 tttcctcggg ctccaggagt tgctgtcgta tttcgacccg cagatcgccg gggtgacggt
     1621 gccgaccatc accgggatga tcgggttcat gatggtcccc tacgtcgaca agaacccctc
     1681 gcaccggccc agcgaccgca agttcgccat cgccctctac tcgaccttcc tcgccggggc
     1741 cgccaccctc accatcatcg gggttctgtt ccggggcagg ggcttcaact tctcctatcc
     1801 gtggaaggac ggagtcttct tcgacgacct caaggactgg gtgcagttcg aatgagtgcg
     1861 accaatctcg cgctgatcgc catcgctgtg gtcgcgatat ttggcgctat cggcgcgttc
     1921 acagtcgcct ttcgccggtc ccagggcggg gcccgcaagc agacggaccc aacttcgggt
     1981 gtctccgacg ag
//
LOCUS       DATITW010000158         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_248085, whole genome shotgun sequence.
ACCESSION   DATITW010000158 DATITW010000000
VERSION     DATITW010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248085"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VLS86_02185"
     CDS             <1..575
                     /locus_tag="VLS86_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49330.1"
                     /translation="DVDMARSLELLTTMVDRAQAMAGPQGGLMFKAWEAWIRAREGDV
                     DEALNRLHALSSQMMVRPLVDTVMASVLLDARLFDDSAPFIAETRAYADWGGINALPP
                     HLDRLEGAARLSAEDPSGLAILERARSAFDDLGMKWEVARTDLWLTEGLIETGDRGKA
                     AAALDSTRPVLDELASSLEIERARLLLERL"
     gene            complement(576..1274)
                     /locus_tag="VLS86_02190"
     CDS             complement(576..1274)
                     /locus_tag="VLS86_02190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HSO49331.1"
                     /translation="MKLGLLICDHVRPEFLEVSGDYDDMFRRLFADHRDVEVVTYDAI
                     GGELPASPAECDAWLTTGSRYSVNDDQRWIRDLEEFVRLVAGTDVPFIGICFGHQLIA
                     RALGGTVVRSDRGWGVGVKEVELKPDLGLGGSYRLLNSHQDQVESVPPGGEILGWNEH
                     CPVSMLGVGDTIVGIQGHPEFETAYSSALMESRRGHEIAEDTVDAGLASLQEGSESAR
                     LADWILDFAKKKSV"
     gene            complement(1277..>2357)
                     /locus_tag="VLS86_02195"
     CDS             complement(1277..>2357)
                     /locus_tag="VLS86_02195"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSO49332.1"
                     /translation="DTFHGQTIPGNADGTLMTFREPIGVCAAITPWNFPMLILSWKTA
                     PALAMGNSVVVKPAEVTPLTALALADLAIEAGLPPGVLNVVTGKGSEAGDALVRHPKV
                     GKISFTGSTEVGAGVMATAASGIKRVSLELGGKSATVVFADAELDECVESSVFAVYDN
                     AGQDCCARSRILVEKGIFDEFAERFVARAGSIVVGDTDDEATEMGPLITPSHRETVES
                     YLAIGESEGATLVIGGERIGERGNYLSPAVFTGARPGMRIVEEEIFGPVVALTPFADE
                     EEAIRLANDSVYGLSGSIWTRDVGRALRVARRFETGMLSINSSSSVHIEAPFGGVKAS
                     GIGREQGMVALEHYSEYKSVFIANH"
BASE COUNT          434 a          796 c          729 g          398 t
ORIGIN      
        1 gggacgtgga catggcccgg tcgctcgagc tgctgaccac gatggtcgac cgtgcccagg
       61 ccatggccgg cccccagggc gggctcatgt tcaaggcatg ggaggcgtgg atccgggccc
      121 gtgaggggga tgtggacgag gctctcaacc gcctgcacgc gctgtcatcc cagatgatgg
      181 tgaggcctct ggtcgacacg gtgatggcca gtgtcctact cgatgcgagg ctgttcgatg
      241 actccgcgcc gttcatcgcc gagacgcgtg cctatgcgga ctggggcggg atcaacgccc
      301 tgccgccaca tctggatcgg ctggagggag ccgcccggct ctccgcggaa gacccgagtg
      361 ggctcgccat cctggaacgg gccaggtcgg ccttcgacga tctcggcatg aaatgggagg
      421 tcgccaggac cgatctctgg ctcaccgagg ggttgatcga gaccggcgac cggggcaagg
      481 ccgcggccgc actcgactcg acacgaccgg tcctcgacga gctcgcctcg tcgctggaga
      541 tcgagcgggc gcgattgctc ctcgagcggc tctgatcaga ccgacttctt cttcgcgaaa
      601 tcgagtatcc aatcggcgag acgagccgat tcggagccct cttggagaga ggcgagaccg
      661 gcatcgaccg tgtcctcggc gatctcgtga ccccgcctcg actccatgag cgctgacgaa
      721 taggcggtct cgaactcggg atgaccctga atgccgacga tggtgtcacc cacgcccagc
      781 atcgacaccg ggcaatgctc gttccaaccc aggatctcac cgccgggcgg cacagactcg
      841 acctggtcct gatggctgtt caagagccga taggagccgc ccagaccgag atcaggcttc
      901 agctcgacct ctttgactcc aacaccccaa cctcgatccg acctgaccac cgtcccaccc
      961 agggctcggg cgatcagttg atggccaaag cagatcccga tgaacggcac atcggtgcca
     1021 gccaccaacc ggacgaattc ctccagatca cggatccaac gctggtcgtc gttgaccgag
     1081 taccgcgagc cggtcgtcag ccatgcgtcg cactcggcag gactggctgg cagctctccg
     1141 ccgatcgcgt cgtatgtcac cacttccaca tctcggtggt cggcgaacag acgacggaac
     1201 atgtcgtcgt agtcccccga gacctcgagg aactcgggtc gaacgtgatc acagatgagc
     1261 agtccgagct tcatggtcaa tggttggcga tgaagaccga cttgtactcc gagtagtgct
     1321 cgagggcaac catcccctgc tcccgcccga ttccggaggc cttgaccccg ccaaacgggg
     1381 cctcgatatg gacactggac gatgagttga tcgaaagcat cccggtttcg aaccgtctcg
     1441 ccactcgcag agcacgaccc acgtctcggg tccagatcga gccggagagc ccgtagaccg
     1501 agtcattggc caaccggatc gcctcctctt cgtcggcaaa gggagtcagg gccaccaccg
     1561 ggccgaatat ctcttcctcg acgatccgca tcccgggtcg ggcgccggtg aagaccgccg
     1621 gggagagata gttgccacgt tccccgatgc gctcaccccc gatgaccagc gtggcgcctt
     1681 cgctctcacc gatcgccagg tatgactcga ccgtctcccg gtgcgacgga gtgatcagcg
     1741 gtcccatctc ggtggcctcg tcgtcggtgt cgccgaccac gatcgacccg gcgcgggcga
     1801 cgaagcgctc tgcgaactcg tcgaatatgc ccttctcgac gaggatccgg cttcgggcac
     1861 agcagtcctg accggcgttg tcatacacgg cgaacaccga ggattcgacg cactcgtcga
     1921 gctcggcatc agcgaagacc acagtcgccg atttgccgcc gagctccagg gacaccctct
     1981 tgatccccga ggccgcagtc gccatcaccc cggcgcccac ctcggtggaa cccgtgaacg
     2041 agatcttgcc cactttcgga tgtcggacca gggcatcacc cgcctcggat cccttcccgg
     2101 tcaccacgtt cagcactccc ggcggcaatc ccgcctcgat ggcgagatcg gccagggcga
     2161 gggcggtcaa tggggtgacc tctgccggct tgaccacgac cgagttgccc atggccaggg
     2221 caggcgctgt cttccaggac aggatcagca tcgggaagtt ccagggggtg atggcggcac
     2281 agaccccgat cggctcccgg aaggtcatca gagtcccgtc ggcattgccc gggatggtct
     2341 gaccatggaa cgtgtcc
//
LOCUS       DATITW010000159         3840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_408377, whole genome shotgun sequence.
ACCESSION   DATITW010000159 DATITW010000000
VERSION     DATITW010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408377"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1212
                     /locus_tag="VLS86_02200"
     CDS             151..1212
                     /locus_tag="VLS86_02200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSO49333.1"
                     /translation="MRITFVGPVPPLRGGIAHHSSRLIDALRSQGHDVQVISWRAQYP
                     WFLYRGEQRDEGVPPYPGARFVLRWWNVVSWWRARRMSRSSDLLVFPYVTPFLAVPQW
                     FMAMGSRRVAAMVHNALPHERMPFERSLAKLAMGNVQLLVTHGEGVADDIRSLGISAD
                     IEVVSMPPTLDVEPSPLPERPPLRLLFFGYVRPYKGLKVAIASVAHLVELGVDVSLDV
                     VGDFWEPVDEYSALIERLGLEDRVHLKVGYVADDELGRALADHHIVVAPYLEDSLSGV
                     VPLAFGAGRPVVSTLVRGVAEQVDDNINGVLVAPGDPIALAEGLLKAAEDLTALARGA
                     AASSTSWGSVAKALTKPFD"
     gene            1334..2401
                     /gene="gmd"
                     /locus_tag="VLS86_02205"
     CDS             1334..2401
                     /gene="gmd"
                     /locus_tag="VLS86_02205"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSO49334.1"
                     /translation="MRKKALITGITGQDGSYLAQYLLEKDYEVHGIKRRTSSFNTVRI
                     DHLHEEPNQPNRQFFLHHGDLTDTLSLMHVLQAVQPDEVYNLGAQSHVAVSFEEPEYT
                     ANSDALGTLRLLEGIRILGLAERTRFYQASTSELFGYAEESPQTEATAFHPRSPYGVA
                     KLYAHWITINYREAYGLFACNGLLFNHESPVRGETFVTRKITRGLARIRFGLQDCLYM
                     GNLNARRDWGHARDYVEAQWLILQQEEPDDFVIATGEQHSVREFVEMAAREMGMSISW
                     HGSGAEEVGVDDRSGQTVIRVDPRYLRPAEVDDLLGDASKARAKLGWKPMTSFSELVR
                     EMVATDLQVAEQDAAVLGGAS"
     gene            2441..3439
                     /locus_tag="VLS86_02210"
     CDS             2441..3439
                     /locus_tag="VLS86_02210"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSO49335.1"
                     /translation="MSGSGRKALITGVRGQDGWYLAKTLLELGYEVIGTSHRADEESM
                     RIGDHDLPVTHLDLAEMSRIEHLVSTQGFDEIYNLASRSSSAQLFDDVITTSEINGVA
                     VARILESIKRHSPTTRFCQASSSEVFAGAEQALQDESTPRIPRNAYGAAKAFADHLVT
                     AYRSEHRLFACSAVLFSHESPRRHPHFVVRKITQGAARIATGDTESVTLDNLESRRDW
                     GYAPDYMDAMRRMLQPDEPRDYVIATGQGHTVQDVCQVAFDHVGLDWRNHVEVSPRSG
                     RLPEIAGRLGDARRAAADLGWTPTLGFAEMIREMVDADLALLQTRSARTGQDALTD"
     gene            3450..>3840
                     /locus_tag="VLS86_02215"
     CDS             3450..>3840
                     /locus_tag="VLS86_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSO49336.1"
                     /translation="MCGNPELTPILDLGEQALTGVFPKSSDRHVEVGPLRLVKCHGSG
                     SCGLVQLEHTYSFDELYGEDYGYRSGLNPTMVRHLQGKVDRILSSHDLPDDALVVDIG
                     SNDGTTLRAYPDRYLRVGIDPTASKFLD"
BASE COUNT          717 a         1238 c         1211 g          674 t
ORIGIN      
        1 cgaccatgca gccaattcgg aatgtcggca agccgccgca aaccgatgac gctgcgactg
       61 ggcagatagg aaacgagagt ccgggtcagc ggtgtcgctc gcatcgccgg ggacgctaac
      121 aaggggtggt ggtgaattat cgtcccgccg gtgcgaatca cctttgtggg gccggttccc
      181 ccgctccggg gaggcatcgc ccatcactcc tcccggctca tcgatgcgct tcgcagtcag
      241 ggacacgatg tccaggtgat cagttggcgc gctcagtatc cctggttcct gtatcgaggt
      301 gaacagcggg acgagggggt ccccccgtat cccggggcgc gattcgtgct ccgctggtgg
      361 aacgtcgtct cctggtggcg ggcacggcgc atgtcccgga gctcggacct cttggtcttt
      421 ccctatgtga ccccgtttct cgcggttcca cagtggttca tggcgatggg atcccgtcgc
      481 gtggcggcca tggtgcacaa cgccctcccc catgagcgca tgccgttcga gcgctccctg
      541 gccaagctcg ccatgggcaa cgtccagctt ctggtcactc atggcgaagg agtggcggac
      601 gacatccgca gccttgggat ttcggcggat atcgaggtgg tcagcatgcc gcccacgctc
      661 gacgtcgagc cgtcacccct tccggagcgg cctcctctgc gtctcttgtt ctttggctat
      721 gtccgcccct acaagggtct caaggttgcg attgcctcgg tggcccacct tgtcgagctc
      781 ggagtcgatg tgagtctcga cgtggtcggc gacttctggg agccggtcga cgaatactcg
      841 gctctcatcg agcgtctcgg tctcgaggat cgtgtccatc tcaaggtcgg ctacgtggcc
      901 gacgacgaac tgggccgggc cctggccgat caccacatcg tggtcgcccc atacctcgag
      961 gactcactct ccggtgtggt gcccctggca tttggcgcag gacggccggt ggtttcgacc
     1021 ctggtcagag gagtagccga gcaggtggac gacaacatca acggcgttct cgtcgccccg
     1081 ggtgacccga tcgccctcgc cgagggtttg ttgaaagcag ccgaagacct gaccgctctg
     1141 gccagggggg cggcagcatc ctccacatcc tggggatcgg tggcgaaagc gctgaccaag
     1201 ccgttcgact agcagcgttc ggctacggtg tgcgatcgtt ggcgaacctc gcattcgccg
     1261 acggggccgc ggtccgtctc ccgggcacgg ccacctgctc gcaccaggct catcagccca
     1321 acgagagagt cacgtgagga agaaagcgct cataaccggg atcacaggtc aagacggctc
     1381 gtacctcgcc cagtacctgc tggaaaagga ttacgaagtc cacggcatca aacgtcgcac
     1441 ctccagcttc aacaccgtcc gcatcgacca ccttcacgaa gagcccaatc agccgaaccg
     1501 gcagttcttc ctccatcacg gagacctgac agacaccctg agcctgatgc acgtgctgca
     1561 ggcggtccaa ccggacgagg tgtacaacct gggtgcgcag agccacgtcg cggtcagctt
     1621 cgaggagccc gaatacaccg ccaactcgga tgctctgggg acgcttcgct tgctcgaagg
     1681 gatccgcatc ctcgggttgg cggaacggac caggttctat caggcgtcga cttcggagct
     1741 gttcggctat gccgaagaga gcccacagac cgaagcaacg gccttccacc cccgcagtcc
     1801 ctacggggtg gccaagctct atgcccattg gatcacgatc aactaccgcg aggcctatgg
     1861 gctgttcgcg tgcaacgggc tcctgttcaa ccatgagtcg ccggtgcgtg gagagacatt
     1921 cgtcacccgc aagatcaccc gtggattggc tcggatcaga ttcgggctcc aggactgcct
     1981 ctacatgggc aacctgaacg cacgccgcga ctgggggcat gcccgggact acgtcgaagc
     2041 ccaatggctg atcttgcagc aggaagagcc agacgacttc gtcatcgcca ccggtgaaca
     2101 acacagcgtc cgcgagttcg tggagatggc cgcccgcgag atggggatga gcatcagttg
     2161 gcatggctcg ggcgcggagg aggtgggcgt cgacgaccgc tcgggccaga ccgtgatccg
     2221 ggtcgacccg agatacttga gaccggcgga agtcgacgat ctgctcggag atgcaagcaa
     2281 ggctcgggcc aagctcggct ggaagccgat gaccagcttc tccgagctcg ttcgcgagat
     2341 ggtcgcaacc gatctgcagg tcgccgagca ggatgctgcc gtcttgggag gagcgagttg
     2401 acctccccgc gattgctcgg accgtggggc agtgcgggcg atgtccggta gcggccgcaa
     2461 ggcgctcatc accggtgtgc gaggtcagga cggctggtac ctggcaaaga cccttctcga
     2521 gctcggctat gaagtgatcg ggaccagcca tcgcgccgac gaggagtcga tgcggatcgg
     2581 cgaccatgac ctgccggtga cgcatctcga tctcgccgag atgagccgga tcgagcacct
     2641 ggtgtcgact caaggattcg acgagatcta caacctggcc tctcgctcat ccagcgcgca
     2701 actcttcgac gacgtcatca ccaccagcga gatcaacggg gtggcggtgg cccggatcct
     2761 ggaatcgatc aagaggcatt ctccgacaac acgcttctgt caggcatcga gcagtgaggt
     2821 ctttgccggt gccgaacagg cccttcagga cgaatccacc ccgaggatcc cacgaaacgc
     2881 ctatggggcg gccaaggcct tcgccgacca cctggtgacc gcctatcgca gcgagcaccg
     2941 gctgttcgcc tgctcagccg tgctgttctc gcacgagagc ccgcggcgcc accctcattt
     3001 cgtagtccgc aagatcaccc agggagcggc caggatcgcc accggggaca cagagtcggt
     3061 gaccctcgac aatctcgagt cgagacgtga ttggggctat gcgcccgatt acatggatgc
     3121 gatgcgacga atgctgcagc ccgacgagcc ccgggattac gtgatcgcaa ccgggcaggg
     3181 acacaccgtg caggacgtct gtcaggtcgc cttcgaccac gtgggtctcg actggcgcaa
     3241 ccacgtcgaa gtctcgccgc gatcgggacg cctgcccgag attgccggtc ggttgggcga
     3301 cgcacggcgc gccgcggcgg atctcggctg gacgcccacg ctcggcttcg ccgagatgat
     3361 cagggagatg gtggacgccg acctggcgct gctccaaacg cgatctgcca ggacaggaca
     3421 agatgcactc acagattgag gtctgccggg tgtgcggcaa cccggagctg acaccgatac
     3481 tcgacctggg ggaacaggcc ctaaccggag tgttccccaa gtccagcgac cgacacgtcg
     3541 aagtcgggcc actcagactc gtcaaatgcc acggatccgg cagctgcggt ctcgtccagc
     3601 tcgagcacac ctacagcttc gatgagctgt atggagagga ctacggctac cgctcgggtc
     3661 tgaaccccac gatggtccgc cacctccagg gcaaggtcga ccggatcctc tcgtcacacg
     3721 acctccccga cgacgcgctg gtggtggata tcggcagcaa tgacggcacc accttacggg
     3781 cctatccgga ccgttacctg agagtcggca tcgaccccac cgcgtcgaag ttcctcgact
//
LOCUS       DATITW010000160         1505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_288448, whole genome shotgun sequence.
ACCESSION   DATITW010000160 DATITW010000000
VERSION     DATITW010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288448"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1505
                     /locus_tag="VLS86_02220"
     CDS             <1..>1505
                     /locus_tag="VLS86_02220"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HSO49337.1"
                     /translation="AHRTSPTNIGLYLLAVLSAREFGWIGLEETVTRLENTVATLNTL
                     ERFQGHFLNWYDTKSCEPLAPRYVSTVDSGNLAGHLLAVSQGCQRLRHLAPGSEVVAG
                     INDAGLLMQHAASLLAANDDIEVVAPRELDRALVEFLTSLHSRDAAGFERLGEILAEA
                     ETVVDIARTLATQKGAEELAIWAEALLATVRGHLHDHRSLAEGTDREYLSRRLAALAE
                     RTADLAAEMDFSFLMDPDNKLLAIGYRVAEKALDQSHYDLLASEARLASFVGIAKGDL
                     PPSHWFRLGRPMTPMGAEAALISWSGSMFEYLMPLLVMRSPPESILDTTYRAVVRRQI
                     EYGSERQVPWGISEAAFAARDIHLVYQYRAFGVPGLGFERGLSEDLVVAPYATLLATM
                     VEPRDAVVNLTRLNSLGALGSYGYYEALDFTPTRLPEDGETVVVRAYMAHHQAMSLVS
                     LTNVIFDGFCRDLFHSDPSVRAAELLLHERVPRDVEVARPRAEEVAESARP"
BASE COUNT          253 a          495 c          473 g          284 t
ORIGIN      
        1 gcacaccgca cctctcccac caacatcggg ctctacctgc tggcggtgtt gtcggctcgg
       61 gagttcggct ggatcggcct cgaagagacg gtcaccagac tcgaaaacac ggtcgccacc
      121 ctcaacacgc tcgaacggtt ccagggtcat ttcctcaact ggtacgacac gaagtcctgt
      181 gagccgctgg cgccccgtta tgtgtcgacc gtggatagcg gcaacctggc cggccacctg
      241 ctggcagtgt cccagggctg tcagcgcctt cgccatctcg caccgggctc cgaggtagtg
      301 gccggcatca acgacgcagg cctgttgatg cagcacgcgg ccagcttgtt ggccgccaac
      361 gacgacatcg aggtggtcgc accccgcgaa ctggaccggg ctctggtgga gtttctcacc
      421 tcgctgcaca gccgggatgc cgccggattc gagcgtctgg gggagatcct cgccgaagcc
      481 gagaccgtgg tcgacatcgc ccggaccctg gccactcaga agggtgctga ggaactggcg
      541 atttgggccg aagcgttgct ggcgaccgtg cggggccacc ttcacgacca tcggtcatta
      601 gccgagggga ccgaccggga atatctctct cgccgtcttg ccgcactggc tgagcggaca
      661 gccgatctgg cggcggagat ggacttctcg tttctcatgg accccgataa caagctgctg
      721 gcgattggct atcgggtcgc cgagaaggcc ctcgatcaga gccattacga cttgctcgcc
      781 tccgaggccc ggctggccag cttcgtaggc atcgccaaag gtgaccttcc ccccagccat
      841 tggttccgtc tcggtcggcc gatgactccg atgggagccg aggcggcgct gatctcatgg
      901 tcaggctcga tgttcgagta cctgatgccc ctgctggtga tgcgctcccc acccgaaagc
      961 atcctcgaca ccacctaccg ggccgtcgtc cgtcgccaga tcgagtacgg gagcgagcgc
     1021 caggtccctt gggggatttc cgaggcagcc tttgcggcac gagacatcca cctcgtctat
     1081 caataccggg cctttggcgt cccggggctc gggttcgaac ggggtctcag tgaagacctg
     1141 gtggtggcgc cctacgcaac cctcctggcg accatggtcg aaccgcgaga tgcggtggtg
     1201 aacctgactc ggctcaacag cctgggggcg ctgggcagct acggctatta cgaggcactc
     1261 gacttcaccc caacccgcct ccccgaggac ggagagaccg ttgtagtgcg cgcctatatg
     1321 gctcaccatc aggccatgtc gctggtttca ttgaccaacg tcatctttga cgggttctgc
     1381 agagacctgt tccattcgga cccctcggtt cgagccgccg aactgttgtt gcatgagagg
     1441 gttccccgcg atgtggaggt ggcgcgcccg cgggccgagg aagtcgccga gtcggcccgt
     1501 ccctt
//
LOCUS       DATITW010000161         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_8069, whole genome shotgun sequence.
ACCESSION   DATITW010000161 DATITW010000000
VERSION     DATITW010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8069"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VLS86_02225"
     CDS             complement(<1..1037)
                     /locus_tag="VLS86_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSO49338.1"
                     /translation="MVGQCQVCGNSAIEPFFSMGQIPLSSCVLLGDRQAAMEFGRGDL
                     ELAVCGRCGFIQNRLFDPGLVDYTMPYEESQAFSPRFTFFQDELISRLVTDHRLHGKT
                     IMEIGSGKGAFLETICRVAGAKGIGIDPAGPTSRIGTGVKITLIGAPFDETNTHLTGD
                     LICCRHTLEHIQPVGRFVANVANSLRRTPGAAAFFELPDTERILEEGAFWDVYYEHCS
                     YFTMTSLAHLFRSKGLGDLKVERGFDDQYLLLEAREGPSNGPPDPHEVEQMVERCRSF
                     GDATSREVDRWRGLIAKAGAGGQTVVVWGAGSKAVGFLSAVSLDEGIRAVVDINPFKQ
                     GSFLPGSAHEIV"
     gene            complement(1031..1675)
                     /locus_tag="VLS86_02230"
     CDS             complement(1031..1675)
                     /locus_tag="VLS86_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HSO49339.1"
                     /translation="MPLGISVGDRPLQVLCLGAHSDDIEIGAGATLLTLASNLDAILV
                     TWVVFSAPERRDAEARHGAELFAPGAEIRLHDFPDARFPMHLDPLKDVFVELGRQLQP
                     DLIFTHTGHDLHQDHGVVSRLTWQTFRDHLILEYEVPKFEGDLQTPNLYVEVEADNAH
                     AKARHLMTAYPSQISKPWFDTDTFLGLMRIRGIECRSRSGFAEGFHVRKATIAW"
     gene            complement(1675..>2052)
                     /locus_tag="VLS86_02235"
     CDS             complement(1675..>2052)
                     /locus_tag="VLS86_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HSO49340.1"
                     /translation="SYLAWFEKHSAVAAFISVRPPHSYHVATADESGVVTDLMPVGEI
                     DQWINAGFFIFRSEIFDYISPGEDLVVEGFQRLISKGLLVTYRYRGFWAPLDTFKEKV
                     LLDELAATGRAPWELWKTERDPG"
BASE COUNT          412 a          637 c          635 g          368 t
ORIGIN      
        1 acgatttcat gggcagatcc gggcaggaag ctgccctgtt tgaaagggtt gatgtcgact
       61 accgcacgta tcccctcgtc gagcgaaacc gccgacagga acccaaccgc cttggagccg
      121 gctccccaga cgaccacggt ctggcctccg gcgccagcct tggcgatgag cccgcgccat
      181 cggtcgacct ctcgactggt ggcgtcaccg aaactcctgc accgttcgac catctgctcc
      241 acctcgtgcg ggtcgggtgg cccgttggac ggcccctccc tcgcctcgag caataggtac
      301 tgatcgtcga agcctcgctc cactttgaga tcgcccaagc ccttggatcg gaacaggtgg
      361 gcgagagatg tcatggtgaa gtaagagcag tgctcgtagt acacgtccca gaaggcaccc
      421 tcctcgagga ttcgctcggt atccggaagc tcgaagaagg cggcagcgcc cggcgtcctc
      481 cgaagtgagt tcgccacgtt cgcaacgaac cgtccaacag gctggatgtg ctcgagagtg
      541 tgtcggcaac aaatcaggtc cccggtcaaa tgagtgttgg tctcatcgaa aggcgcccca
      601 atgagggtga tcttcacgcc cgtgccgatc cgactggtgg gaccggcggg atcgatcccg
      661 attcccttcg ccccggccac ccgacatatg gtctcgagaa atgccccctt cccagacccg
      721 atctccatga tggtcttgcc atgcaaccgg tggtcggtca cgagccggga aatcagctcg
      781 tcctgaaaga acgtgaaacg aggagagaac gcctggctct cctcgtaagg catcgtgtag
      841 tcgaccagcc cagggtcgaa gaggcgattc tggatgaacc cgcaccgacc gcaaaccgcc
      901 agctccaggt caccccgtcc gaactccatt gcggcctgtc ggtcccccaa caggacgcag
      961 ctcgacaacg ggatctgccc catggagaag aacggctcga tggccgagtt gccgcagacc
     1021 tggcactgcc ctaccacgcg atggtggctt ttctcacgtg aaatccctcg gcaaagcccg
     1081 accgagagcg gcactcgatc ccccggatgc gcatgagacc gagaaaggta tcggtgtcga
     1141 accagggttt cgagatctgc gacgggtatg ccgtcatcag gtggcgggcc ttggcgtggg
     1201 cgttgtccgc ttcgacttcc acgtagagat tcggagtctg caggtcgccc tcgaacttgg
     1261 gaacctcgta ctcgaggatg agatggtccc ggaatgtctg ccaggtcaat ctgctcacca
     1321 cgccgtgatc ctgatggaga tcgtggccgg tatgggtgaa tatcagatcg ggttgaagtt
     1381 ggcgaccgag ctctacgaag acgtccttca gagggtcgag gtgcatgggg aatcgggcgt
     1441 cggggaagtc gtggagccgg atctcggcac cgggggcgaa aagctcggcg ccgtgcctcg
     1501 cctcggcgtc gcgtctctcg ggagccgaga agacgaccca ggtgacgagg atcgcgtcca
     1561 ggttcgaagc aagagtgaga agggtcgccc cagcgcctat ctcgatgtcg tcgctgtgtg
     1621 cgcccaggca gaggacctgg agcggacgat cgccgaccga gatcccgaga ggcatcaccc
     1681 gggatctcgc tccgtcttcc acaactccca cggggcccgc ccggtggcag ccagctcatc
     1741 gaggaggacc ttctccttga aggtgtccag tggcgcccag aaccctcggt atcgataggt
     1801 caccaacagg cccttggaga tcaaccgctg gaatccctcg accacgagat cctcgccggg
     1861 agagatgtag tcgaagatct ccgagcggaa gatgaaaaac ccggcattga tccattggtc
     1921 gatctcgcca accggcatga ggtcggtgac gacgccgctc tcgtcggcag tcgccacgtg
     1981 ataactgtgt ggggggcgaa ccgatatgaa tgcggcgact gcgctgtgtt tctcgaacca
     2041 cgccagatag ct
//
LOCUS       DATITW010000162         1657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_729013, whole genome shotgun sequence.
ACCESSION   DATITW010000162 DATITW010000000
VERSION     DATITW010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729013"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VLS86_02240"
     CDS             complement(<1..274)
                     /locus_tag="VLS86_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HSO49341.1"
                     /translation="MADAVIRIDDRELQLDVIEGVEGDVAVDISRLRPELGAIPLDRG
                     FGNTAESVSGVSYVGGEEGVLHYRGYPIEQLAESATFLEVVYLLQYG"
     gene            complement(296..>1657)
                     /locus_tag="VLS86_02245"
     CDS             complement(296..>1657)
                     /locus_tag="VLS86_02245"
                     /inference="COORDINATES: protein motif:HMM:NF015706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HSO49342.1"
                     /translation="TRDQVVATIHKGIISGDIHPVLVTSAERMIGIDLLAEFIVDYGP
                     NPLERSLPKLTDGEAFSPVTDGPVLAYVFKSVSDPFVGRISLFRIYSGSVKADQELEL
                     ARGGKVRLHNLFKLQGKDHHDVPDLPAGGVGAVAKVENVRVGDTLRSSGTTVVIEPVS
                     YPRPVAEVAITPRSHHDEEKLSTALHRIEEEDPTVKIERRADTGETILAGLGDSHLDV
                     TVARIHRKFGVEVDTALPVIPYRETVTATASAEGKHKKQSGGRGQFGVALVKFAPKPR
                     GSGYEYIDSIKGGSIPRQFIPAVDKGIQEALQRGILAGFPVIDISAEVYDGKFHAVDS
                     DEMSFRMAGIQALRAATGDLRPILLEPVMKVTVTVPEEHLGDVMGDINSKRGRVLGME
                     GEGMLRSVVAEVPMAEIQQYAAELRSLTSGRGNFEVVFDHYAEVPHAEAQQVIAAARS
                     TDD"
BASE COUNT          269 a          555 c          530 g          303 t
ORIGIN      
        1 ctccgtactg gagcaggtag acgacttcga ggaacgtggc gctctccgcc aactgttcga
       61 tcgggtagcc ccgatagtgg agcacgcctt cctcgccacc cacataagag actccagaca
      121 cgctctcggc cgtgttccca aagccacgat cgagggggat cgccccgagc tcggggcgta
      181 gccggctgat gtcgaccgcg acgtctcctt cgactccctc gatgacgtcg agttgcagct
      241 ctctgtcgtc gatgcggatc acagcgtcag ccatatccag tcctccctag agccatcagt
      301 cgtcagtgga gcgggcagcg gcgatcacct gctgtgcctc ggcatggggc acctcggcgt
      361 agtggtcgaa caccacctcg aagttacccc gtcccgaggt caatgatcgc agctctgcgg
      421 cgtactgctg aatctcggcc atcgggacct cggcgaccac cgatctcagc atcccctccc
      481 cttccatgcc cagcacccgg cctcgcttgg agttgatgtc gcccatgacg tcgccgagat
      541 gctcctcggg gaccgtgacc gtgaccttca tcaccggctc cagcaggatc ggcctcaggt
      601 cgccagtggc ggcccgaagc gcctggatgc ccgccatcct gaacgacatc tcgtccgagt
      661 cgacggcgtg gaatttgccg tcgtagacct cggcggagat gtcgatgacc ggaaagccgg
      721 ccaggatgcc tcgctgcagc gcctcctgta tccccttgtc gacagcgggg atgaactggc
      781 gggggatcga gccgcccttg atcgaatcga tgtactcgta ccccgatccc cggggctttg
      841 gggcgaactt gaccagggcc acaccgaact gcccccgtcc tcccgactgc ttcttgtgct
      901 tgccctcggc cgaggcagtg gcggtgacgg tctcccggta ggggatgacc gggagcgcgg
      961 tgtcgacctc gaccccgaac ttgcggtgga tgcgtgccac cgtcacgtcg agatggctgt
     1021 cgcccagccc ggcgaggatc gtctcgccgg tgtccgccct ccgctcgatc ttgaccgttg
     1081 ggtcctcctc ctcgatgcgg tgcagagcgg tcgacagctt ctcctcgtcg tgatgggatc
     1141 tcggtgtgat ggcgacctcg gcgacgggtc gcggatagga gaccggctcg atgaccaccg
     1201 tcgtgccgga gctgcgcaac gtgtcgccca cccggacgtt ctccactttg gccacggcgc
     1261 ctacgccacc cgccgggagg tcgggcacgt catggtgatc tttgccctgc agcttgaaca
     1321 ggttgtggag acggaccttc cccccgcgcg ccaactcgag ctcctggtcg gccttgaccg
     1381 agccggagta gatgcggaac agggagatcc ggcccacgaa cggatcggag accgacttga
     1441 agacgtaggc gagcaccggc ccgtcggtga cgggcgagaa cgcctcgcca tcggtgagct
     1501 tgggaagtga gcgctcgagc gggttggggc cgtagtcgac gatgaactcg gcgagcaggt
     1561 cgatgccgat catcctctcc gccgaggtga ccaggaccgg atggatgtcg ccggagatga
     1621 tccccttgtg gatcgtggcc accacctggt cgcgggt
//
LOCUS       DATITW010000163         1924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_729045, whole genome shotgun sequence.
ACCESSION   DATITW010000163 DATITW010000000
VERSION     DATITW010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729045"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VLS86_02250"
     CDS             complement(<1..577)
                     /locus_tag="VLS86_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HSO49343.1"
                     /translation="MPVRSHKNRRVVEAARLHRARERRDRGLTLIEGPHLLEEALAAG
                     VTPVEVFAREGDEPTRELAAARQIDLVIVDDLGLARVAGTKSPRGPVASIEIPRNGSL
                     GDAPGVVVSWGIGDPGNVGTLIRTAAAFGWAFAYTSDTADPWAPKVLRAGAGAHFRTG
                     LMPIESMAQLDSLGYQTLATVVDGGMAPSALV"
     gene            complement(601..951)
                     /gene="rplT"
                     /locus_tag="VLS86_02255"
     CDS             complement(601..951)
                     /gene="rplT"
                     /locus_tag="VLS86_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HSO49344.1"
                     /translation="MARVKRAVHAKKSHKKVLDRAKGYKGARSRRYRTANEQVMHAMQ
                     DAYRDRRARKGEFRRLWIARINAAARQNGTTYNQLIAGLKANDIEVDRKMLAEMAVND
                     PGAFAQLVEASKQG"
     gene            complement(970..1164)
                     /gene="rpmI"
                     /locus_tag="VLS86_02260"
     CDS             complement(970..1164)
                     /gene="rpmI"
                     /locus_tag="VLS86_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HSO49345.1"
                     /translation="MPKMKTHSGAKKRIQVTGSGKLMRGQSGRGHLKLAKGKNVFRRR
                     AGQVELAPGDAKTAKKLLGR"
     gene            complement(1183..1710)
                     /gene="infC"
                     /locus_tag="VLS86_02265"
     CDS             complement(1183..1710)
                     /gene="infC"
                     /locus_tag="VLS86_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HSO49346.1"
                     /translation="MNGAIRAKEVRVVAPDGSQIGVKKLNEALWLAEQLDLDLVEVAP
                     NAKPPVCRLMDYGKYKYEQSVKQREARKKQTRTTIKELRMKVRIGDHDFDITKRKAKD
                     FLAEGDKVKVTIRFRGREQERPGFGKDLLDRLAEELGDVSVVEQVPKIEGRSMTMVLA
                     PAKKARATAESSEEN"
BASE COUNT          285 a          642 c          580 g          417 t
ORIGIN      
        1 cgaccagggc cgacggtgcc atcccaccgt cgaccacggt ggccagcgtt tggtatccga
       61 ggctgtcgag ttgggccatc gactctatgg gcatcagccc ggttcggaaa tgagccccgg
      121 ctccggcacg cagcaccttg ggggcccagg gatctgcggt gtccgaggtg taggcgaagg
      181 cccagccgaa ggcagccgct gtccggatga gggttccgac gttgcccggg tcgccgatcc
      241 cccacgacac caccacaccg ggagcatctc cgagagatcc gttgcgggga atttcgatcg
      301 atgccaccgg gcctcgtggg ctcttggttc ccgctacccg ggcgagcccc aggtcgtcga
      361 ctatgaccag gtcgatttgc ctggcggccg ccagctctcg ggtgggctca tcgccctccc
      421 gtgcgaacac ctccacgggg gtgacacctg cagccagcgc ctcctccagg agatggggac
      481 cctcgatcag ggtcagcccc cgatctcggc gctcccgcgc gcggtgaagc cgggccgcct
      541 ccaccacccg acggttcttg tggctgcgga ccggcatcac tcccggctgt ctacgcgtcg
      601 tcagccctgc ttcgaggcct cgaccagctg ggcgaaagcc ccgggatcgt tgacggccat
      661 ctcggcgagc atcttccgat cgacctcgat gtcgttggcc ttcaacccgg caatcagctg
      721 gttgtaggtg gtcccgttct gacgagccgc ggcgttgatc cgggcgatcc agagacggcg
      781 gaattcgcct ttgcgtgcgc ggcggtcgcg ataggcgtcc tgcatcgcat gcatgacctg
      841 ctcgttggcg gtgcggtaac gcctgctgcg ggcgcccttg taacccttgg ctcggtcgag
      901 gaccttcttg tgtgacttct tggcgtgaac cgcgcgcttt acacgtgcca tctcatacct
      961 cctggattct cagcgaccga gcagcttctt ggcggtcttg gcgtcgcccg gggcgagctc
     1021 gacctgaccc gcccgacgac ggaacacgtt cttgcctttg gccagcttga gatggccgcg
     1081 acctgactgg cctctcatca atttgccgga accagtgacc tggattcgct tcttggcacc
     1141 gctgtgggtc ttcatcttgg gcacagcgtc ctcctgttgt ttctaattct cttcgctgct
     1201 ctcggcggtg gccctcgcct tcttcgccgg ggccagcacc atcgtcatgc tgcgaccctc
     1261 gatcttgggc acttgctcca cgacgctgac atcgcccaac tcctcggcga gacggtcgag
     1321 aaggtccttc ccgaacccgg ggcgctcttg ttccctgccg cggaaccgga tcgtcacctt
     1381 gaccttgtct ccctcggcca ggaagtcctt ggcctttcgc ttggtgatgt cgaagtcgtg
     1441 atcgccgatc cgcactttca tgcggagctc tttgatcgtg gtccgagtct gcttcttgcg
     1501 agcctcgcgc tgcttgacgg actgctcgta cttgtatttc ccgtaatcca tcaatcggca
     1561 caccggcggt ttggcgttgg gtgccacttc gacgagatcc agatcgagtt gttctgcaag
     1621 ccacagggct tcgttgagtt tcttgacccc gatctgggag ccatccggtg cgactacccg
     1681 cacctctttt gctcgtatcg cgccgttcac acggagctcg ttggtgatgc cgacacctcc
     1741 tgttgagtgc aaatgaaaga gcagcaagcg aaaacttgct gctcaagagc ggctattgcc
     1801 gatctggtgg accacggctc gcctcgatcg tcttcggccg ggcggccggg tggggaagca
     1861 gcagcttcca ctcttgctat cgattgtccc ccgaaggcta ccagcgacgg aggctctatc
     1921 acca
//
LOCUS       DATITW010000164         2180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_48200, whole genome shotgun sequence.
ACCESSION   DATITW010000164 DATITW010000000
VERSION     DATITW010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48200"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1431
                     /locus_tag="VLS86_02270"
     CDS             136..1431
                     /locus_tag="VLS86_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HSO49347.1"
                     /translation="MTDRVEFFSSERWTAGIIGLGYVGLPLLITAVNNGLSAIGFDVS
                     EARVADLTAGRSHVDDITDEALRAAIDSGARFTSDPEALAEADAIFICVPSPLGRNRQ
                     PDLSYIEAAADTVSAIARPGQLVSLESTTYPGTTEDYLVPAVEKAGLTVDEDVWVAFS
                     PERVSPGDEMKTGDIPKVVGGITEISGQVAEAAYSRLAPFVHRVSSARAAEMSKLLEN
                     TYRAVNIGLINEMAQLAHKLGIDIWEVVDAAATKPFGFQPFYPGPGVGGHCIPLDPQY
                     LAWRAKEANFTTRFIDTAEQVNSKMPAYNVTRISELLNTRGLPILGTQMLGVGIAYKP
                     NIADDRESAALEVLKELTERGARVAVLDPIVGSERIRSYGYEPVDESEDLSRFEIAVI
                     LTDHSDIDYSKLAREIGVVFDTRGVYRRLGVEASNVESL"
     gene            1428..>2180
                     /locus_tag="VLS86_02275"
     CDS             1428..>2180
                     /locus_tag="VLS86_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSO49348.1"
                     /translation="MTRPAILVLTTVHPPDDTRIRERLIRSIEHLGEVTYASRSPGPS
                     DTAGLSWVELPGGRAGRNWRASRLVVGRAWDLVILHDPETIPAGLVARMLRRTRVVFD
                     VHEDLPSQIESKEWVPSWARPLVRGIARGLYWAAERGLDITLSEPGYQRLFARPHPVF
                     ANYPRSSSFPDPEPVGDGSAIYVGDITRARGIEEAMEASAKAGVSLVAVGRVDPTLAV
                     RLQTGAASRGTELTLSGHLANPVALERVSVASV"
BASE COUNT          379 a          704 c          717 g          380 t
ORIGIN      
        1 cgaggcgcag atcactgttg ggtgcaaccc acccgccaac gatcgctact gccccggcga
       61 ccgggtgaca cgcgggcaga tggctgcctt cttcaagagg gcttgggggc cctgagcggt
      121 cctactatca gttttgtgac agaccgagtc gagttcttct cctccgagcg atggactgca
      181 ggcatcatcg gtctcggcta tgtggggctc ccgcttctca tcaccgcggt caacaacggc
      241 ctttcggcga tcggattcga cgtctccgaa gcccgggtcg ccgatctgac cgcgggtcgc
      301 tcgcatgtgg acgacatcac ggacgaggcg ttgcgcgctg cgatcgattc cggggcgagg
      361 ttcacctccg atcctgaggc gctggcagaa gccgatgcca tcttcatctg tgtccccagc
      421 ccactgggcc ggaaccgcca gccggatctc tcgtacatcg aggcggcggc ggacacggtc
      481 tccgccatcg cccgccccgg ccagttggtg agtctggagt cgaccacata tccgggcacc
      541 accgaggatt atctggttcc ggcggtggag aaggcgggcc tgaccgtgga tgaggacgtc
      601 tgggtcgcct tctcgccgga aagggtgtcg cccggggacg agatgaagac cggggacatc
      661 cccaaggtgg tgggggggat caccgagatc tcggggcagg tggcagaagc tgcgtattcg
      721 cgactggctc cattcgtgca cagggtctcc tcggctcggg cggccgagat gagcaagctg
      781 ctggagaaca cctaccgggc ggtcaacatc gggttgatca acgagatggc ccaactggcg
      841 cacaagctgg gcatcgacat ctgggaggtg gtcgatgcgg ctgcaaccaa gccatttggc
      901 ttccagccct tctatcccgg ccccggcgtt ggtggccatt gcattcccct cgatccccag
      961 tatctggcat ggcgggcgaa ggaggccaac ttcacgaccc ggttcatcga caccgccgaa
     1021 caggtcaact cgaagatgcc tgcctacaac gtcacccgga tatcagagct tctcaacacc
     1081 cgggggctgc ccatcctcgg gacacagatg ctcggggtcg gcatcgccta caagccgaac
     1141 atcgccgacg atcgggaatc ggccgcactc gaggtgctga aggagctgac cgagcgcggc
     1201 gcccgggtgg cggtcctcga tccaatcgtc gggagcgagc ggatcaggtc atacggatac
     1261 gagcccgtag acgagtcgga ggacctgtcc cggttcgaga tcgcagtgat cctcaccgac
     1321 cattcggaca tcgactactc gaagctggca cgcgagatcg gggtcgtctt cgacacgcga
     1381 ggtgtgtatc gacggctggg ggtggaggcc tccaacgtag agtcgttatg acccgtccgg
     1441 cgatcctggt cctgacgacg gtccacccac cggatgacac caggatccgg gagcggctga
     1501 tcaggagcat cgagcatctc ggcgaggtga cttacgccag ccggagcccg gggccttctg
     1561 acaccgccgg actgagctgg gtcgagctcc ccgggggccg agccggacgc aactggcgcg
     1621 cttcccgctt ggtggttggg cgagcctggg acctggtcat cctccacgat ccggagacca
     1681 tccccgccgg tctggtcgcc cgaatgctgc gtcgcaccag ggtggtcttc gacgtgcacg
     1741 aagaccttcc ctcgcagatc gaatccaagg agtgggtccc ctcctgggcc cggcccctgg
     1801 tcagggggat cgcccgcggt ctctattggg ctgcggagag gggattggac atcaccctct
     1861 ccgagcccgg ctaccagaga ctgttcgcac ggccgcaccc tgtcttcgcc aactatccca
     1921 gatcatcctc attccccgat ccggaacctg tgggcgacgg ctcggcgatc tatgtcgggg
     1981 acatcacccg agctcggggg atcgaagagg ccatggaggc ttcggccaag gcgggggtgt
     2041 ccctggtggc ggttggccgg gtcgacccga cactggcggt cagactgcag accggagccg
     2101 cctcccgcgg aaccgagcta acactcagcg gccaccttgc caatccagtg gcgctggaac
     2161 gcgtctccgt ggcctccgtg
//
LOCUS       DATITW010000165         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_288523, whole genome shotgun sequence.
ACCESSION   DATITW010000165 DATITW010000000
VERSION     DATITW010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288523"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VLS86_02280"
     CDS             <1..662
                     /locus_tag="VLS86_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013019252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSO49349.1"
                     /translation="GPAIGIGTINAFVRRLQASVRHLERMIRADVPDPERHKIRLDPN
                     QVTVVDLHNLNDRAKRFVVGVTLKRAFDDKERAGQSEELLLVVLDELNKYAPREGTSP
                     IKEVLLDVAERGRSLGVILIGAQQTASEVERRIIANSSIRVVGRLDAAEAAREEYGFL
                     PSVHRQRATIIKPGTMILSQPELPVPLVLEFPFPAWATRASEALAAPSSAGPEDPFEG
                     L"
     gene            721..>1618
                     /gene="sbcD"
                     /locus_tag="VLS86_02285"
     CDS             721..>1618
                     /gene="sbcD"
                     /locus_tag="VLS86_02285"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HSO49350.1"
                     /translation="MTVKILHTSDWHVGRRIRGRDRSDEHRAVLAELVTIAAGAEVQL
                     VVVAGDVFDRSAPTPAAEQIVWRGLLDLAEVAPVVVIAGNHDNPGRLEAVSPLLELGR
                     IVVCSEPSRPDRAGVMTYDELGIKVAMLPFVSQRGIVRAEQIMGSDPDQHAGAYEERI
                     KRLIEALTASMTPDTVNLLAGHLTVHGAQEGGGERQAHIFGYAVPASVFPGHLSHVAL
                     GHLHRQQKVPHGGSVWYSGSPLQLEFGEVDDRKGALLVEASPGLPSKVTAVPLTSGRR
                     LVTLRGTLEQVRAHADDVEGAYV"
BASE COUNT          257 a          528 c          556 g          277 t
ORIGIN      
        1 cgggcccggc gatcggcatc ggcaccatca acgctttcgt gcgaagactc caggcctcgg
       61 tccgtcacct ggaacggatg atccgggccg acgtccccga ccccgagcgg cacaagatcc
      121 gactcgaccc caaccaggtg accgtcgtgg atctgcacaa tctcaacgat cgagcgaagc
      181 gcttcgtggt cggggtgacc ttgaagcgtg ccttcgacga caaagagagg gccggccagt
      241 ccgaggagct gctcctggtg gtgctcgacg agttgaacaa gtacgccccc cgtgagggga
      301 ccagccccat caaggaggtt ctgctcgatg tcgccgagcg agggcgctct ctgggggtga
      361 tcctgatcgg ggcccaacag acggcgtcgg aggtggagcg caggatcatc gccaactcct
      421 cgatccgggt ggtgggccgg ctcgacgcgg ccgaagcggc gcgggaggag tacgggtttc
      481 tcccctcggt gcaccgccag cgggcgacca tcatcaagcc ggggaccatg atcctgagcc
      541 agcccgagtt gccggtgccc ctggtgctgg aattcccgtt cccggcatgg gcaaccaggg
      601 catcagaggc gcttgcggcg ccttcatcgg caggtcccga agacccgttc gagggtctgt
      661 agcgggccgg ggcggggtgg ctccccgaat ctgtcacccc tgctctttac gatcgacctt
      721 gtgacagtga agatccttca tacctccgat tggcatgtcg gtaggcggat ccggggcagg
      781 gaccggtccg atgagcaccg tgctgttctc gccgagttgg tgacgatcgc ggccggcgcc
      841 gaggtccagc tggtggtggt ggccggcgac gtctttgaca ggtcggctcc gacccccgcc
      901 gcggagcaaa tcgtgtggag gggcctgctc gacctggccg aggtggcgcc ggtcgtggtc
      961 attgccggca accacgacaa cccgggtcgg ctggaggcgg tgtcgcccct gctcgagttg
     1021 ggccggatcg tcgtctgctc cgagccatct cgacccgatc gggccggggt gatgacctat
     1081 gacgagctcg ggatcaaggt cgccatgctc cccttcgtct cccagcgcgg catcgtgcgc
     1141 gccgagcaga tcatgggctc cgaccccgat cagcatgccg gcgcctacga ggagcggatc
     1201 aagaggctga tcgaggccct caccgcttcg atgacccctg acaccgtgaa tctgctggcg
     1261 ggccatctca ccgtgcacgg cgcccaagag ggcgggggag agcgccaggc ccacatcttc
     1321 ggttatgcag tgcccgcatc ggtgttcccc ggtcatctct cccatgtcgc cctcggccat
     1381 cttcatcgcc agcagaaggt gccacatgga ggctcggtct ggtattcggg gtcgcctctc
     1441 cagctcgaat tcggggaggt ggacgatcgc aagggggcgt tgttggtcga ggcgagccca
     1501 gggctcccct ccaaggtgac ggccgtcccg ctgacctcgg gtcgacggct ggtcaccctc
     1561 cggggcacgc tggaacaggt ccgggctcac gccgatgacg tggagggcgc ctacgtca
//
LOCUS       DATITW010000166         1742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_128222, whole genome shotgun sequence.
ACCESSION   DATITW010000166 DATITW010000000
VERSION     DATITW010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128222"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1486)
                     /locus_tag="VLS86_02290"
     CDS             complement(5..1486)
                     /locus_tag="VLS86_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSO49351.1"
                     /translation="MDDRLYIGGRFTPALAGATIEVRNPHDGDKLADVAEARAEDVDL
                     AVEAASEAFPVWSATSAMDRGRLLLRLADAIEADAEELARIETLDTGHPIRDTSVLDV
                     PRTAATFRYFGGMADKFQGSIPPVEAGFLNHVYRRPLGVVGQIVPWNFPLMFTSWKLG
                     PALAAGNTVVYKPAELTPLSTLRLARLIDDAGFPPGVVNIVPGYGHTAGARIGEHSGI
                     AKVSFTGSTLTGRSLVAASAGNLKRLQLELGGKGANIVFEDADLDAALAGSAFAIFHN
                     QGQACIAGSRLLLHEAVADEFLERFLRLTKAIKLGDPMDRATEMGPLTSPGHRDRVLD
                     YVKVALDEGGEILSGGRAPADPSLANGCYVEATVVRAKPADRVCHEEVFGPFVTVSTF
                     STEDEAIGIANGVEYGLSGSIYTRDVNKAFRAMREFETGIFYVNSSTIGAEVHLPFGG
                     IKNTGNGHREAGIAGLEVFTEWKSIYVDFSGRLQKAQIDTHEN"
     gene            complement(1563..>1742)
                     /locus_tag="VLS86_02295"
     CDS             complement(1563..>1742)
                     /locus_tag="VLS86_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HSO49352.1"
                     /translation="GTVRAKVVMRAGDVAAMPADIRHQGYSRKRSMLLVWENADSALP
                     ELIATGQTPTYPAEF"
BASE COUNT          289 a          585 c          557 g          311 t
ORIGIN      
        1 agcgctaatt ctcgtgcgtg tcgatctgcg ccttttgcag tcttcccgag aagtcgacgt
       61 agatcgattt ccactcggtg aagacctcca ggcccgcgat ccccgcctcg cgatgcccgt
      121 tgcccgtgtt cttgatcccg ccgaagggca ggtgcacctc ggcgccgatg gtcgaggagt
      181 tcacgtagaa gatgccggtc tcgaactcgc gcatcgcgcg gaacgccttg ttaacgtcgc
      241 gagtgtagat cgagccggac agcccgtact cgaccccgtt ggcgatcccg atcgcctcgt
      301 cctcagtcga gaaggtcgac acggtcacga aggggccgaa cacctcctcg tggcacaccc
      361 ggtccgcagg cttcgcccgc accactgtcg cctctacgta acagccgttg gccaacgacg
      421 gatccgcggg cgcccttccc ccgctgagga tctcgcctcc ctcgtccaac gcgaccttga
      481 cgtagtcgag aactcgatcc cggtgaccag gcgaggtgag agggcccatt tccgtggccc
      541 ggtccatcgg gtcgcccagc ttgatcgcct tggtgagtcg gaggaatcgc tcgaggaact
      601 catcggccac cgcctcatgg agtaggagtc tcgacccggc gatacatgcc tgcccttggt
      661 tgtggaagat ggcgaaggcc gagccggcca gtgcggcgtc gagatcagcg tcctcgaaga
      721 cgatgttcgc ccccttgccc cccagctcca gctggaggcg cttcagattt cccgcagagg
      781 ccgcaaccag gctgcggccc gtgagggtgg agccagtgaa cgacactttg gcgattccgg
      841 agtgctcgcc gatccgggcg ccagcggtgt gcccgtaccc ggggacgatg ttgaccactc
      901 cgggcggaaa gccggcatcg tcgatcagcc tggccagacg cagcgtggac aggggtgtca
      961 gctcggccgg cttgtagacc acggtgttcc cggcagcgag ggccggaccc agcttccagc
     1021 tggtgaacat gagcgggaag ttccagggca cgatctggcc caccacgccg agcggccgcc
     1081 tgtagacgtg gttgaggaaa ccggcctcga ccggtgggat agagccttgg aacttgtccg
     1141 ccattccgcc gaagtaccgg aacgtggccg cggtacgggg cacgtcgagg accgaggtat
     1201 cccttatcgg gtgtccggtg tcgagagtct cgatcctggc tagctcctcg gcgtcggcct
     1261 cgatcgcgtc ggcgagccgg agcaggagac ggccacgatc catggccgac gtcgccgacc
     1321 aaaccggaaa cgcttcggac gcagcctcga cggctaggtc tacgtcctca gccctggcct
     1381 ctgcaacgtc cgccagcttg tccccgtcgt gtgggttgcg cacttcgatc gtggctccag
     1441 ccaacgcagg ggtgaaccgg ccaccaatgt agaggcggtc atccactcac tcacctccgg
     1501 cgtgggccgt gtcggaccgc gttggtcgcg ctatgagcct catccgcgcc gggtcggttc
     1561 ggctagaact cggccgggta tgtgggcgtc tgaccggtgg caatcagctc aggcagcgct
     1621 gagtcggcgt tctcccaaac caggagcatc gaccgcttgc gtgagtagcc ctggtgtctg
     1681 atgtcagccg gcatcgcagc gacatcgccg gccctcatca ccaccttggc cctcaccgtc
     1741 cc
//
LOCUS       DATITW010000167         1593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_528951, whole genome shotgun sequence.
ACCESSION   DATITW010000167 DATITW010000000
VERSION     DATITW010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528951"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VLS86_02300"
     CDS             <1..305
                     /locus_tag="VLS86_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49353.1"
                     /translation="MPTRRSHVTEWILGIVGVLAAGVGAWMYYVPADWFLGGLAEGWY
                     FGMFIAAGLLLAAAFGLFARMTYDDDREWTTPVIVSTVLSAAALAGAIAFTVILII"
     gene            complement(524..1117)
                     /locus_tag="VLS86_02305"
     CDS             complement(524..1117)
                     /locus_tag="VLS86_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017297299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HSO49354.1"
                     /translation="MPSIVARAQPGSPNGKEVLTPLTRGAWRDWLARNGGREEGLWVV
                     YRKKSSDLEGPLYDDLVEEALCFGWIDSRVQRVDDERRMQWFSPRRKGGVWSSLNKAR
                     IERLIGEGLMSAAGQRVIDEAIADGSWSQTDEVDALVIPPDLETALDVVPGTRDAFDA
                     LPVSTRRQHLWWIHSAKRAVTRSNRIEELMRHLSEDD"
BASE COUNT          278 a          530 c          479 g          306 t
ORIGIN      
        1 ggatgccgac aaggcgctcc cacgtgaccg agtggattct cgggattgtt ggtgtgctgg
       61 cagcaggagt cggcgcgtgg atgtactacg tgccggccga ctggttcctt ggtgggcttg
      121 ccgagggttg gtacttcggc atgttcatcg cagccggact tctgctcgcg gccgcattcg
      181 ggctcttcgc tcggatgacc tacgacgacg accgggaatg gacgaccccc gtgatcgtca
      241 gcacggttct gtccgcggct gcccttgcgg gcgccatcgc atttaccgtg atcctgatca
      301 tctagacgcc caatggaacc ggggctcacg atcgagtccc ggttccgcgt tcgggcggcc
      361 agatccctat cgccgggacc tgaacgtcgg gcagggagcc gagtacatga gccccttgat
      421 gtgccgttct cgacggcccc gcgaggtcgt gatcatcccg cagcgcggcc gaacgtcggg
      481 cacatcgaag acccggacgt ctatcctgct ccggcccggc acctcaatca tcctccgaca
      541 gatgacgcat caactcctct attcggttgg acctcgtaac ggctcgcttc gcgctgtgga
      601 tccaccacag atgctgcctt ctggtcgaga cgggcaaggc atcgaatgca tctctcgttc
      661 cgggcaccac gtccaacgcg gtctcgaggt ccggtgggat cacaagggcg tcgacctcgt
      721 cggtctgcga ccatgatccg tcggcgatcg cctcgtcgat caccctttga ccggctgccg
      781 acatgagccc ttccccgatc aggcgctcga ttcgagcctt gttgagcgac gaccagaccc
      841 caccttttct ccgcggggag aaccactgca tccttcgctc gtcgtccacc cgctgcaccc
      901 gactgtcgat ccaaccgaag cagagggcct cctccacgag gtcgtcgtac agcggacctt
      961 ccaggtcact ggacttcttc cggtacacca cccagagccc ctcctcccgc ccgccgtttc
     1021 gagccagcca atctctccac gcccccctcg ttagaggagt gaggacctct ttgccgttcg
     1081 gggagccagg ttgggcacgg gccacaatgc tcggcatcgg gaggcgaacc taccccttgc
     1141 ttcgctcata gcgtggtctc ccgagggaat acgcacgtcg acaagcacca cccccgagga
     1201 ggaaagaccc ggcatcaggt gggccaatgg actcctcagt ccttcttccg ccttatagga
     1261 ggtgaccctc tcgtgggtgg agctgacggg atttgaaccc atgacttcct cgttgcgaaa
     1321 gatgcggtca aaaccctctg accaggggaa acgaccgatc cagaccgtcg tgtggagcgg
     1381 gtgtggggcg agcgacgtga ggtgttgtga ggcgaagtga acgcccggcc cagcggcgtg
     1441 gccctaggac agggcttcac gccgaggatg aggacgaatc cggcggacct catcgccctc
     1501 attctgctgc gcgtggtaca ggtcccatcg catctgaccg ttcatccaga agccactcga
     1561 cgtgccgaag taccgcgaga gcctcagggc agt
//
LOCUS       DATITW010000168         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_488821, whole genome shotgun sequence.
ACCESSION   DATITW010000168 DATITW010000000
VERSION     DATITW010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488821"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VLS86_02310"
     CDS             <1..293
                     /locus_tag="VLS86_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSO49355.1"
                     /translation="ALFCANDNSAVGALDAIRNAGLRVPEDIALAGFDDLAIASEIDP
                     PLTTMRPDISGIGQEAARNLLSLLENPDGGPRRVLLPTELVIRQSTIGDFPG"
     gene            393..1847
                     /locus_tag="VLS86_02315"
     CDS             393..1847
                     /locus_tag="VLS86_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSO49356.1"
                     /translation="MKKTKSIALVVVLFLILGACGDSGTSSSTTAAGSEGSTTAAPAG
                     STTAAPSGEAVEIRWFVGLGAGDQPEQKAAQEAVVAEFNESQDEIELVLEIVDYDVAY
                     TTLANQIATGDAPDIIGPVGRDGSNGFAGLYLDIEPLIESTGVETSQWATAAVDNQRE
                     ADGTLVGLPFGSFPSAIYFNRDLFAEAGLPEPPQEYGPDGVTVYGEGTEYEGTWDWAK
                     VQEIAEILTVDGNGVDATDPAFDRTTATQWGFHWQFTTGLFQNGSFWGAGYPMADDGT
                     ADIPAIWEEEWKWFHANVWERGISPDQTVLDAGDNLFQGGNIAMAATHLWYTCCIQSD
                     TIAGDFWDYGVMPSNDGVVTANLHADTFRILDSTEHPDEAFTAMYYLLTEAALDLLTA
                     YGAAPADPALMDAYLAAQDERYPQGVNWQTILDGANYADNPSHETFLPGWSEYKIRLK
                     ELETGLLSNPDIDLDAEIAQVESDLSAIFAENSG"
     gene            1985..>2589
                     /locus_tag="VLS86_02320"
     CDS             1985..>2589
                     /locus_tag="VLS86_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSO49357.1"
                     /translation="MPWILGFFLWYAIPMAASLWFSFTDYDLVSDEPMQFVGLENWVR
                     LFNDADVHQSVLVTLKFGAIALPVAILFPMAIAYLLVAKSLKGREGFRALFFMPSIIP
                     FVAAVLVFNGVFNGQIGWVNKILAIFGIDGPEWFYDEFWIYPSLVFIGLWAVGNAILI
                     FIAAMKGVPSDLYAAARIDDAGEWGSFRHITVPFTTPIIFY"
BASE COUNT          489 a          790 c          819 g          491 t
ORIGIN      
        1 acgccttgtt ctgtgccaac gacaacagtg ctgttggggc attggacgcg attcgaaatg
       61 cggggcttcg agtgcccgag gatattgccc tggccggctt cgacgatctg gcgatcgcgt
      121 cggagatcga cccacccctc accacgatgc gcccggacat ctccgggatc ggccaggagg
      181 cagcccgcaa tctcttgagt cttctggaaa accccgatgg tgggccgcgt agagtgcttc
      241 ttccgaccga gttggtcatc agacagtcga cgattggaga tttcccggga tagcccggga
      301 gagaaagcgg ctccgcccgt ccccggggca gagccacacc ggtcaagccg atcggtgata
      361 tatccggggt cgaaggagga gaacggatag aaatgaagaa aactaagagt atcgccctcg
      421 tggtggtcct tttcttgatt ctcggcgctt gtggtgactc cggtacttcc agcagcacca
      481 cagcggctgg gagcgagggg tccacgacgg cggcgccagc cgggtcaacg acggcggcgc
      541 cttccggcga ggccgtcgag atccgatggt tcgtcgggct cggtgccggt gaccagccgg
      601 agcagaaggc agcccaagag gcagtggtcg ccgaattcaa cgagtcgcag gatgagatcg
      661 agctcgtgct cgagatcgtc gactacgacg tcgcatacac gaccctggca aaccagatcg
      721 ctacgggcga cgccccggac atcatcggtc cggtaggccg cgacggctcg aatgggttcg
      781 ccgggctcta cctggacatc gagccgttga tcgagtcgac cggggtggag acctcacaat
      841 gggcgaccgc cgccgtggac aaccagcggg aagccgacgg gaccttggtc ggtctcccgt
      901 tcgggtcctt cccgtcggcc atttatttca accgggacct cttcgcggag gccggtctgc
      961 ccgaaccgcc acaggagtat gggccggatg gcgtgactgt ctacggtgag ggcacggaat
     1021 acgagggaac ctgggactgg gccaaggtcc aggagatcgc cgagatcctg accgttgacg
     1081 gcaacggggt ggacgccacg gatcccgcct tcgaccgcac gactgcgacc cagtggggct
     1141 tccactggca gttcaccacc ggtctcttcc agaacgggtc gttctggggc gccggttacc
     1201 cgatggcgga tgacggcaca gccgacatcc cggcgatctg ggaagaggag tggaagtggt
     1261 tccacgccaa cgtgtgggag cgaggcatct caccagacca gaccgtattg gatgcgggcg
     1321 acaacctgtt ccagggcggg aacatagcaa tggcggcgac tcacctctgg tacacgtgct
     1381 gcattcagag cgacaccatt gccggagact tctgggacta cggggtcatg ccttccaatg
     1441 acggggtggt cacggccaac ctgcatgcgg acaccttccg catcctggac tcgaccgagc
     1501 atcctgacga ggccttcacg gccatgtact acctcctcac cgaggcggct ctggacctgc
     1561 tgacggccta cggtgcagcg ccggcggacc cggcgctgat ggacgcttac ctggcagccc
     1621 aagatgagag gtacccgcaa ggggtcaact ggcagaccat cctcgatggc gccaactatg
     1681 cggacaaccc gagccacgag accttcctcc ccggatggtc ggagtacaag atccggctca
     1741 aggagctcga aaccgggctc ctgtccaatc cagatatcga tctggatgct gagatcgccc
     1801 aggtggaaag cgacctgagt gcgatcttcg ccgaaaacag tggatagcta acgtcacggt
     1861 cgatggtatg tccgtgatcg tcgatgaggg ggtggccggt tcggccaccc cctcatcacc
     1921 ctcatcacca cgcaaacgct catccctggc aaagagaaga ggccgcgccg ggctttggtt
     1981 cgtcatgccc tggatactcg ggttcttcct ctggtacgcg atcccgatgg cggcctccct
     2041 ctggttctcg ttcaccgact acgacctggt gagtgacgag ccgatgcagt tcgtgggact
     2101 ggagaactgg gtcaggttgt tcaacgacgc cgacgttcac cagtcggtct tggtgaccct
     2161 caagttcggg gcgatcgccc tccccgtcgc gattctgttc ccgatggcaa tcgcatatct
     2221 gttggtcgcg aagtcactca aggggcggga gggctttcgc gctctgttct tcatgccctc
     2281 gatcatcccg ttcgtggccg cggtcctcgt cttcaacggg gtcttcaacg ggcaaatagg
     2341 ctgggtgaac aagatcctcg ccattttcgg catcgacggg cccgaatggt tctacgatga
     2401 gttctggatc tacccctccc tggtgttcat cggtctctgg gcggtgggga atgcgatcct
     2461 catcttcatc gccgcgatga agggggtgcc gtctgatctg tatgcggcag cgcgcatcga
     2521 cgacgctggg gagtggggtt ccttccggca catcaccgtc ccgttcacca ccccgataat
     2581 cttctacaa
//
LOCUS       DATITW010000169         2545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_649281, whole genome shotgun sequence.
ACCESSION   DATITW010000169 DATITW010000000
VERSION     DATITW010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649281"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VLS86_02325"
     CDS             complement(<1..813)
                     /locus_tag="VLS86_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HSO49358.1"
                     /translation="MRIILVTGKGGVGKTTVAASTAISAADAGHRTLITSTDPAHSLS
                     DALSVALGSDPSPVVPGLDGQQIDTQRQLERYWGAIRRQLMDVLDWGGAGGIEAEEFL
                     VFPGMDELFALLEVNHHARSGDYDVIVVDCAPTAETLRLLSLPEVLSWYFEKVLPTER
                     RLMRAARPILTRFTDLPLPQDEVFTAAQSVFVSIEEVKELMSDPAITSARLVVNPEKM
                     VIDEARRTYSYLGLFGYGVDGVVVNRVLPEEVADPYFERWRAIQKGHLDSIED"
     gene            complement(816..1256)
                     /locus_tag="VLS86_02330"
     CDS             complement(816..1256)
                     /locus_tag="VLS86_02330"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSO49359.1"
                     /translation="MTEGTFQTLEIDAPAEDLYDVAADIASYPEWATGVKEVEVLEST
                     PDGQVQRARFVIEGFVKEIEYVLRYTHDRPNTLSWVAEADSKDVKSLEGSYTFNPVDG
                     ATEVVYALTVEPSFVVPGFLRRQAEKQIVTTALRGLRKRAAGSG"
     gene            complement(1303..2076)
                     /locus_tag="VLS86_02335"
     CDS             complement(1303..2076)
                     /locus_tag="VLS86_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSO49360.1"
                     /translation="MLVLSDVHFALEPLRKVAASGETLLILGDLVNLTDYRTGEGAVA
                     EVLGIEFSRASAAARGRGDFQQMRALWLERVGDDLDEIRLAIGKAIDSQYERVAEALN
                     GGSGYVIHGNVDRPGSLQRSLPAGYEYVHGDVREIDGARFGFIGGGAATPLQAEGEVS
                     DETVEELLEVIGPVDVLCTHVPPALGPLRTDVVTGRAERGSVPILNYLEKHAPRLHLF
                     GDVHQPQASTWRVGRTRCRNVGYFRATGRPVRLDTATIE"
     gene            complement(2060..2374)
                     /locus_tag="VLS86_02340"
     CDS             complement(2060..2374)
                     /locus_tag="VLS86_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49361.1"
                     /translation="MVKIYSIALALGFFGLLVVILGGAFADNLGRDKDDPSRSLGVAG
                     RSVIGGLVGFGMAGLSAEFSTFDLTWQTALVVAVLGALVGALWAGYASREGSRSEDAG
                     PL"
BASE COUNT          463 a          869 c          793 g          420 t
ORIGIN      
        1 gtcctcgatc gaatcgagat ggcccttctg gatcgcccgc caccgctcga agtagggatc
       61 ggcgacctcc tcggggagga cccgattcac aaccaccccg tcgaccccgt agccaaacag
      121 gcccaggtag ctgtaggtgc gtctcgcctc gtcgatgacc atcttctccg ggttgaccac
      181 caggcgggcc gaggtgatcg caggatcgga catcagctcc ttgacctctt cgatcgacac
      241 gaataccgac tgggcggcgg tgaacacctc atcctgggga agggggaggt cggtgaaccg
      301 ggtcaggatc gggcgagccg cccgcatcag gcgacgttcg gtgggcaaca ccttctcgaa
      361 gtaccagctc aggacctcag gcagcgagag caaccgcaga gtctccgcgg tcggggcgca
      421 atcgaccacg atcacgtcgt agtcacctga tcgcgcgtgg tggttcacct cgagcaaggc
      481 gaacaactcg tccatacccg ggaacaccag gaactcctcg gcctcgatcc cgccggcgcc
      541 gccccagtcc aggacatcca tcagctgtct tcggatcgct ccccagtagc gctccaactg
      601 gcgttgggta tcgatctgtt ggccgtcgag acccggaacc accggagatg ggtccgagcc
      661 cagggcgacc gagagcgcgt ccgacaggga gtgggccgga tcggtggagg tgatcagggt
      721 ccggtggccg gcatcggcag cagagatcgc ggtggaggcg gccacggtgg tcttgcccac
      781 accccccttg ccggtgacga ggatgatcct catcttcagc ccgagcctgc tgctctcttg
      841 cgcaggccgc gcaatgctgt cgtcacgatc tgcttctcgg cttgacgtct cagaaatccg
      901 ggcacgacga agctgggctc gacggtcaag gcgtagacga cttcggtcgc cccgtccacc
      961 ggattgaagg tataggaccc ttccagcgac ttcacatctt tggaatcggc ctccgccacc
     1021 cagctcagcg tgttcggccg atcgtgggtg tagcgcagga cgtactcgat ctccttgacg
     1081 aaaccctcga tcacgaaccg ggctcgctgg acctgaccgt cgggggtgct ctccaggacc
     1141 tcgacctctt tcacaccggt ggcccattcc ggatacgagg cgatgtcggc ggccacgtcg
     1201 tagagatcct cggcgggagc gtcgatctcg agcgtctgga aggttccttc ggtcatggct
     1261 ccgtcagtcg tgtggaggtg ccgagattag ccagactgcc gattattcaa tcgtggccgt
     1321 gtccaacctc accggtctcc cggtcgcccg aaaatagccg acgttccggc atcgggtccg
     1381 acccacccgc caggtgctgg cctgaggctg gtgcacgtct ccaaacaaat gcagtcgtgg
     1441 ggcgtgtttc tcgaggtagt tgaggatcgg aaccgagccc cgctcagctc ggcccgtgac
     1501 cacgtcggtc cgcagtggcc ccagcgccgg cggcacatgg gtacacagca cgtcgacggg
     1561 accgatgact tccagcaact cctcgacggt ctcgtctgac acctctccct cggcttgaag
     1621 cggtgtggcc gccccgcccc cgataaaacc gaaccgggcg ccgtcgatct ctcgcacgtc
     1681 gccgtggaca tactcgtaac ccgccgggag cgaacgttgc agggatccgg gacggtcgac
     1741 attgccatgg atcacgtaac cgcttccacc gttcagggcc tcggccaccc tctcgtactg
     1801 ggagtcgatc gcctttccta tcgccaggcg aatctcgtcg aggtcgtcgc cgactcgctc
     1861 cagccagaga gctctcatct gctggaagtc accccggccc cgggcggctg ccgaggcgcg
     1921 ggagaactcg atgccgagga cttcggcaac cgctccctca ccggtgcgat agtcggtgag
     1981 attcaccagg tcgcccagga tgagcagggt ctctcctgag gccgccacct tgcgcagcgg
     2041 ctccagggcg aagtggacgt cagagaggac cagcatcttc tgacctggat ccctcccggg
     2101 aggcgtaccc ggcccagagc gccccgacca gggcgccgag gaccgcgacc accaatgccg
     2161 tttgccaggt caggtcgaac gtcgagaact cggcagacag accggccatc ccaaacccga
     2221 ccagaccgcc gatcaccgac cggccggcga cgccgagcga tcgggaggga tcgtccttgt
     2281 cccggccgag gttgtcggcg aacgcgccgc cgaggatcac caccagcaaa ccgaagaagc
     2341 cgagggcgag ggcgatcgag tagatcttga ccatgcccgt cggagggtaa cccgccggga
     2401 tgctcagccc gcccgtctct ccgtcggcac cagccgcaga cggggctcca gaccctcacc
     2461 ggcgaggttg gccagcgcgc tcacctcgga gtcgcccagc tccaccgggc catcggcaag
     2521 gagaaaggtc accacacagt cgtca
//
LOCUS       DATITW010000170         1705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_649391, whole genome shotgun sequence.
ACCESSION   DATITW010000170 DATITW010000000
VERSION     DATITW010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649391"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VLS86_02345"
     CDS             complement(<1..498)
                     /locus_tag="VLS86_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HSO49362.1"
                     /translation="MQRHTKIIATLGPAVASEARIRALVEAGMDVARLNFSHGDHELH
                     RALAGWVREAAEETGRSVALMQDVQGPKLRVGEFNNGAVMLEAGRQVVLVPGGGMGDE
                     NLIPVGYEPLLDDVIAGDRVQLSDGLIRCEVVGATEGGLLAEVRVGGILSDHKGVAFP
                     DSRLSL"
     gene            complement(545..>1705)
                     /gene="gatB"
                     /locus_tag="VLS86_02350"
     CDS             complement(545..>1705)
                     /gene="gatB"
                     /locus_tag="VLS86_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HSO49363.1"
                     /translation="VPVCHDGHLDIEIDGATRRIGIERAHMEEDTGKSTHLGAGGRIH
                     DAESTLLDFNRSGVPLVEIVSRPDLRSASESRAYAQELRSIVAELGVSDARLEEGSIR
                     FDANVSIRPMGDEELGTKVEVKNMNSFRSLERAVDFEIERQVALVESGGSVTQETRHW
                     DEEAGVTHSMRTKEGSSDYRYFTEPDLVPMAMSAEWVDRVRAGLPELPAQRRERYRSQ
                     GLDHETAGVLSGVDATLRSIYDDAVLSGAPARAAANWLTGEVVGWLRKRDVEATALPL
                     GGAALAELIAMVDGGLVSSSAAKEVLDGVLSGEGTPQVVAATRDLLQMSDTAELGQIV
                     SDVLAANPQAIAEYRAGEDKVVGYLVGQVMKATQGKADPRAVNQILRDQMAG"
BASE COUNT          272 a          604 c          537 g          292 t
ORIGIN      
        1 cagggagaga cggctgtcgg gaaaggcgac gcctttgtgg tcgctgagaa tgcctccaac
       61 ccgcacctcg gcgagaagcc cgccttcggt ggcccccacc acctcacagc ggatgaggcc
      121 gtcggagagc tggacccgat ccccggcgat gacatcgtcg agcaggggct catagccgac
      181 agggatcagg ttctcgtccc ccatgcctcc gcccgggacc aggaccacct gtctcccggc
      241 ctcgagcatc accgcgccgt tgttgaactc gcccaccctg agctttgggc cctggacatc
      301 ctgcatcagg gccaccgacc gacctgtctc ctcggcggct tccctcaccc agccggccag
      361 ggcgcgatgc agttcgtggt cgccgtggga gaaattgagc cgggcgacat ccatgcccgc
      421 ctcgaccagg gcccggatcc gggcttctga ggccacggcg gggccgagag tggcgatgat
      481 cttggtgtga cgctgcattt cgacctgacg ctaaccagtc atcccccgga gacggggaag
      541 ccgctcagcc cgccatctgg tcgcggagga tctggttcac cgcccgcgga tcggccttgc
      601 cctgggtcgc cttcatcacc tggccgacca aataaccgac caccttgtcc tctcccgccc
      661 ggtattcggc aattgcctgc gggttggcgg cgaggacgtc gctgacgatc tgccccagct
      721 ccgcggtgtc gctcatctgg agcaggtcac gggtcgcggc caccacctgg ggggtcccct
      781 caccggagag caccccatcg agcacctcct tggccgcgga gctggagacc aacccgccgt
      841 cgaccatggc gatcagctcg gcaagggcag caccacccag gggcagggcc gttgcctcca
      901 catcccgctt ccgcagccaa ccgacgacct cgccggtcag ccaattggcg gcagctctcg
      961 ccggggcgcc ggagagaacc gcgtcgtcgt agatcgagcg cagggtggcg tcgacgccgg
     1021 aaagaactcc tgccgtctcg tggtccaggc cctggctccg gtatcgctcc cgacgctgcg
     1081 ccggaagctc gggaagaccg gcccggaccc gatcgaccca ttcggcgctc atcgccatcg
     1141 gcaccaggtc gggctcggtg aagtagcgat agtcggatga gccttccttg gtgcgcatgg
     1201 aatgggtgac acctgcttcc tcatcccagt gtctcgtctc ctgggtcacc gacccacctg
     1261 actccaccaa tgcgacctga cgctcgatct cgaagtcgac ggcccgctcc agggatcgga
     1321 acgagttcat gttcttgacc tcgaccttgg ttcccagctc ctcatcgccc atgggacgga
     1381 tcgacacgtt ggcgtcgaat cggatcgagc cttcctccag cctggcgtcg gacaccccga
     1441 gctcggcaac gatcgatctc agctcctggg cataggccct cgactcggat gcgctgcgga
     1501 ggtcggggcg ggagacgatc tcgaccagcg gcactcccga tcggttgaag tcgaggaggg
     1561 tcgactcggc gtcgtggatt cggcccccgg caccgagatg ggtcgacttg ccggtgtctt
     1621 cctccatgtg ggcccgctcg attccgatgc gccgggtcgc tccgtcgatc tcgatgtcga
     1681 gatggccgtc gtggcagacc gggac
//
LOCUS       DATITW010000171         1998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_88635, whole genome shotgun sequence.
ACCESSION   DATITW010000171 DATITW010000000
VERSION     DATITW010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88635"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1932
                     /locus_tag="VLS86_02355"
     CDS             301..1932
                     /locus_tag="VLS86_02355"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSO49364.1"
                     /translation="MSNRFIRPGSWRRRRQLSWRRRLPLGSFVLAMMLLVPIPVSAVD
                     DNEFEVVLDAEMPGLLDDYGVSGSVVSHIANGSVTWTNAYGIADLDAGTAMSPDMVFE
                     FGSCGKILTAWATMKLVERGAIELDTPVNEYLNRLQIESGRYDSSGVTVRRLLSHTSG
                     LGIHGYVDYSPRRANPPDPVQTLQGAHLLEGIVETLEPGGVSFGRIGLVQEPGSGYRY
                     SGAGYGVLQILIEDVTGEPFATFVQREITDPLGAASVRWEWTSELEARSPTPYGEEGQ
                     PMEHRQLTIHGIGSELGTVEDFARFVAATVAGPNGEPAGRGVLSPETIEMMTTPLSEA
                     GSYQGLGYPLGSLNGHRSVSHGGRNMGWEAFFILDTVTGDGFVVASASNRAGPLHSAI
                     TDLFLDATYGPSIRSSAAPLPGLELLSWVFLAISAVLLVVLALSLLRFVSDVRSGRRA
                     RSDSPSRQSLVRAVPWLLALLFGWYTLYSPLPIYLPAWYPDLWPTRGSGVLMGTLVAG
                     ITYRVATAFFPRLAAAPTPDPAEPSTESRLPAAAR"
BASE COUNT          323 a          634 c          660 g          381 t
ORIGIN      
        1 tgaggatgac atcgtggtct actgcaccag tgatgactgc catgccagcg tcgccctcta
       61 tcacgctctg gtcgaccacg gctacccgaa cgtccatcga tactccggcg ggttgaccga
      121 atgggaggat gccggtctgc cgctggaggg tgagtggtca tcgtgaccgg ctgagcaccg
      181 gggcgctgga gagacgcagt cgccgacaac gcacgccccg aacgttcgac gcagcctcgt
      241 cccggttgaa gcggtcattg gcccctatcg cgggacggac gggggctgta tccacgaggc
      301 atgagcaacc gcttcatccg cccaggttcc tggcggcgga ggagacagct ttcctggcgt
      361 cggcgtcttc cgctcgggag cttcgtcctc gcgatgatgc tcctggtccc gatcccggta
      421 tcggctgtgg acgacaatga gttcgaggtc gtactggacg cggaaatgcc cgggctcctc
      481 gacgactatg gggtctctgg ttccgtggtt tctcacatcg cgaacggatc cgtgacctgg
      541 accaacgcat atgggatcgc cgacctcgac gcgggcacag cgatgagccc ggacatggtc
      601 ttcgagttcg gatcttgtgg gaagatcttg acggcttggg cgacgatgaa gctggtcgag
      661 cggggggcaa tcgagctcga cacgccggtc aatgagtatc tcaaccggtt gcagatcgaa
      721 tcgggccggt atgactcgag cggggtcacg gtccgacggc tcctcagcca cacctccggc
      781 ctcggcatcc acgggtatgt cgattacagc cctcggcgcg ccaatccgcc cgatccggtt
      841 cagaccctcc aaggcgcgca cctcctggag gggatcgtcg agacgctgga gccaggcgga
      901 gtgtcattcg gccgaatcgg tctggtgcag gagcccggat ctggctaccg ctactcagga
      961 gccggctatg gcgtgcttca gatcctgatc gaggacgtga ccggtgagcc tttcgccacg
     1021 ttcgtgcaga gggagatcac cgaccccctg ggcgcggcaa gcgtgcggtg ggagtggacg
     1081 tctgagctcg aggctcggtc tcccactcca tacggtgagg aaggacagcc catggagcat
     1141 cgccagctca cgattcatgg gatcgggagc gagctgggaa cggtcgaaga cttcgcccgc
     1201 ttcgtggcag ccacggtcgc aggccccaat ggtgagcccg cgggtagggg agtgctctcc
     1261 ccagaaacca ttgagatgat gacgacaccg ctgtcggagg ccggctctta tcagggtctg
     1321 ggatatccgc tgggctcgct caacgggcac cgttccgtgt cccacggggg acggaacatg
     1381 ggctgggagg cgttcttcat actcgacaca gtgaccggcg acggattcgt ggtcgcttcg
     1441 gcttccaacc gggccgggcc gctgcacagt gcgatcaccg acctgtttct cgacgcaacc
     1501 tacggaccca gcatccgctc cagcgcggcg ccgctccccg gactggaact gctttcctgg
     1561 gttttcctcg ccatcagtgc cgtgctgctg gtcgtgcttg cgctgagcct cctccgcttc
     1621 gtcagcgatg tgcgatccgg cagacgagcg cgaagcgact cgccgagccg gcaaagcctg
     1681 gtgcgtgccg tgccctggct tctggctttg ttgttcgggt ggtacacgct ctattcgcca
     1741 ttgcctatct atctccctgc ctggtatccc gatctgtggc cgaccagggg cagtggtgtg
     1801 ctcatgggca cgctggtagc cggcatcacc tacagagtcg ccaccgcgtt cttcccccgg
     1861 ctggcagcgg ccccgacacc tgatccggcc gagccgagca ccgagagtcg tctaccggcc
     1921 gctgcccggt aggaccatct gccgcgacgg tgggtgaggg gtggccgcat cggccctggt
     1981 catgtgttgt cgcgcccg
//
LOCUS       DATITW010000172         1519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_449200, whole genome shotgun sequence.
ACCESSION   DATITW010000172 DATITW010000000
VERSION     DATITW010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449200"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VLS86_02360"
     CDS             complement(<1..360)
                     /locus_tag="VLS86_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HSO49365.1"
                     /translation="MRRSSLVVLLVVGALLVPAAVFASHQFNDVPDSHTFHTGISWMK
                     DNNITVGCNPPTNTNYCPDDNVTRGQMATFMKRLADNQVVDAGALGGQTAASYQGSAA
                     GVLISSLTPANVVRTRIA"
     gene            complement(469..1056)
                     /locus_tag="VLS86_02365"
     CDS             complement(469..1056)
                     /locus_tag="VLS86_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49366.1"
                     /translation="MRLVLVFTIVYMVVLFGYGLAVASPLTNLYPGINLLVFAVFAVI
                     HRWARFPLPVIWGIALVGLGNMIGGVILVDGQPLYVAPFIGPVPYDKFFHGAASFVMF
                     FVAWAAMTRFAGAAPHFGGLVLFTFLATMGGGAVVEIAELIGSAAGGGRFNVGDYGNN
                     ALDLVSNAAGAAVGVIVLLVARRSLAPAAIEGEDG"
     gene            1174..>1519
                     /locus_tag="VLS86_02370"
     CDS             1174..>1519
                     /locus_tag="VLS86_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49367.1"
                     /translation="MPILERRPITVAAKKYLAATLVLLATLVLAPIASAQTDEEPSPP
                     PATELTVTTPYPSVAVEAGDQVSFALSVASPSRVEVGLTADGVPEGWTAAFRGGGFEV
                     DSVTAGPGLTPEV"
BASE COUNT          291 a          502 c          475 g          251 t
ORIGIN      
        1 ggcgatccgg gttcggacca cattggcagg agtcagactg ctgatcagga ccccagccgc
       61 gctgccctga taggaggcgg ctgtctggcc accgagggcg ccggcgtcca cgacctggtt
      121 gtcggcgaga cgcttcatga acgtggccat ctgaccacgg gtcacattgt cgtccgggca
      181 gtaattggtg ttggtcggcg ggttacaccc caccgtgatg ttgttgtcct tcatccagct
      241 gataccggta tgaaaagtgt gactgtccgg cacgtcattg aactggtgag aggcgaacac
      301 ggccgccggc acgagcagag ctcccaccac cagcagcacg acaagcgacg atctgcgcat
      361 attgactgac cctccttcgg tctgcagcgg aacatagtcg tcgctccccg aagaggcatg
      421 gagttggatc gatcttcatc ttcgattcac cgaccagccg acctcttgtc acccgtcctc
      481 accctcgatc gccgccggtg ccaggctccg ccgcgcgacg aggagcacga tcaccccgac
      541 tgcggcaccg gccgcgttgg agaccaggtc gagtgcgttg ttcccgtagt caccgacgtt
      601 gaacctcccc ccgcccgccg ccgagccgat cagctccgcg atctcgacca ccgcgccgcc
      661 ccccatcgtc gccaggaagg tgaacaggac cagaccaccg aaatgcggcg ccgccccggc
      721 gaaacgggtc atcgccgccc atgcgacgaa gaacatgacg aacgaagcgg ctccatggaa
      781 gaacttgtcg taggggacgg gcccgatgaa cggggcaacg tacaggggct ggccgtcgac
      841 gaggatcacc ccgccgatca tgttgcccag cccgaccagc gcgattcccc agatcacggg
      901 cagcgggaac ctcgcccagc gatggatcac ggcgaagact gcaaacacca ggagattgat
      961 cccggggtag agattggtga gagggctggc gacggctagg ccgtagccga aaagcacgac
     1021 catgtagacg atggtgaaca cgaggacgag gcgcatcgct cccccagact cgcaccgggg
     1081 cgagcaaccg ggcaaactcc cccacgcttc gctgggcggg acctgccgtt tatcacgtct
     1141 ttaggggtga tcagcgaacg atcgggttgt tttgtgccca ttctcgaaag gagaccgata
     1201 accgtggccg cgaagaagta tctggctgcg actcttgtcc tgttggcgac cctggtgctg
     1261 gcaccgatcg cctcggccca gaccgatgag gaaccctctc ctccgcccgc aaccgagctg
     1321 acggtcacca ctccctaccc ctctgtcgcc gtagaggcgg gagatcaggt gagcttcgca
     1381 ctgtctgtcg cctcccccag tcgcgtcgag gtcgggctca ccgcggacgg agtccccgaa
     1441 gggtggaccg ccgcgtttcg cggtggcggg ttcgaggtcg actcggtgac cgctgggccc
     1501 gggttgacgc cagaagtca
//
LOCUS       DATITW010000173         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_569487, whole genome shotgun sequence.
ACCESSION   DATITW010000173 DATITW010000000
VERSION     DATITW010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569487"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1020
                     /locus_tag="VLS86_02375"
     CDS             52..1020
                     /locus_tag="VLS86_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49368.1"
                     /translation="MFARGRDPYFPAWPDVLQLNAFQPGLRQAVIGTVAGIAGQCDGI
                     RCDMAMLMLNNVFERTWGARAGAKPAGDYWPTVIPAIQGIHREFRFMAEVYWDLEWEL
                     QQQGFDYCYDKKLYDRMEHGGAESVRQHLLADSSYQEGMVRFIENHDEPRAAATFSSG
                     KGRAAAVAILTLPGAKLLHEGQFEGWKVRLPVFLGRRPAEPVDQDLAAFYCNLLKANH
                     HGAFRNGQWRLCERSGWPGTQSCRNHLAWCWVKDEERCLVIVNFSDGTAQALVHAPWD
                     DLGGRTWRLADALSGEVYDRSGDEMRDPGLYVDLPPWGYHVFSLGS"
     gene            1046..1735
                     /locus_tag="VLS86_02380"
     CDS             1046..1735
                     /locus_tag="VLS86_02380"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSO49369.1"
                     /translation="MKAITVEPKKAGTARLEEIPEPDERDGSVLVEAVAVGVCGTDIE
                     IVEGKYGWAPPGKTRLVLGHESLGRVVDPGPRSGFRKGDLVVGIVRRPDPVPCPNCAV
                     GEWDMCRNGRYTERGIKEIDGFMSERWRIEPEYAMKVDSSLGLLGVLIEPTSVVAKAW
                     EQIAAVGQRSFWKPRTVLVTGAGPLLGMFLAILVFSFLAKRFEVAKRYHFESGRRRMF
                     RTMSRQDPGGP"
     gene            complement(1799..>2036)
                     /locus_tag="VLS86_02385"
     CDS             complement(1799..>2036)
                     /locus_tag="VLS86_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSO49370.1"
                     /translation="YVELAQLRFDNGYASYLEVLDAQRSLFNVQLSYTQNQGVLFQAM
                     INLYKAMGGGWVTEADRPTAGAAGPAPKAPPAGK"
BASE COUNT          345 a          612 c          723 g          356 t
ORIGIN      
        1 cccgacgatg cgaggaacga tccggcatcc ttcgtggagg cgggaggaac ggtgtttgcc
       61 cgcggccggg atccgtactt cccggcgtgg cccgatgtgc tccagctcaa cgccttccag
      121 ccggggctcc ggcaggcggt gatcgggacg gtcgcgggga tcgccggaca gtgcgacggc
      181 atccggtgcg acatggccat gctcatgctg aacaatgtct tcgagcgcac ctggggggct
      241 cgtgcgggag cgaaaccggc aggggattac tggccgacgg tcatcccggc gatccagggg
      301 atacatcgag agttccggtt catggccgaa gtgtactggg acctcgagtg ggagctgcag
      361 cagcaggggt tcgactactg ctacgacaag aagctttatg accggatgga gcacgggggc
      421 gccgaaagcg tgcgccagca cctccttgcg gattcttcct accaggaggg aatggtccgg
      481 ttcatcgaga accacgacga gccgagggcg gccgccacgt tttcgtccgg aaaaggacgc
      541 gccgcggcgg tggcgatcct cacgctcccg ggggcgaagc tgctgcacga ggggcagttc
      601 gagggatgga aggtcaggct gccggtcttt ctgggccgtc gcccggcaga gccggtcgat
      661 caggacctcg cggctttcta ctgcaatctt ctgaaggcga accaccatgg tgcctttcgg
      721 aatggccaat ggcgcctctg cgagcggagc ggctggcccg gcacccagag ctgccggaat
      781 cacctggcct ggtgctgggt caaggacgaa gagcgctgcc tcgtcatagt gaatttttcc
      841 gacgggaccg cccaggcgct cgtccatgcg ccgtgggatg acctgggagg cagaacgtgg
      901 cgccttgcgg atgccttgtc aggggaagtc tacgaccgca gtggcgacga gatgagggat
      961 ccgggcctgt acgtcgacct gccgccttgg ggttaccacg ttttctcctt gggcagctaa
     1021 ttcacgggaa tcaagagggg accacatgaa agccatcacg gtggaaccga agaaggcggg
     1081 gaccgcgcga ctggaggaga tcccggagcc cgatgagcgc gacggatccg tgctggtgga
     1141 agccgttgcg gtcggggtct gcgggaccga catcgagatc gtggagggga agtacggctg
     1201 ggcgcctcct ggaaagaccc gtctcgtgct gggccacgaa tcccttggcc gcgtggtcga
     1261 tccgggcccg aggagcgggt tccggaaggg cgatctcgtg gtcggcatcg tccggcgtcc
     1321 cgaccccgtc ccctgcccca actgcgcggt gggggagtgg gacatgtgcc gcaacggaag
     1381 gtacaccgag cgcggcatca aggaaatcga cggcttcatg tccgagcggt ggcggatcga
     1441 gcccgagtac gcgatgaagg ttgactcctc cctcggcctc ctcggagtgc tgatcgagcc
     1501 cacgtcggtg gttgcgaagg cgtgggaaca gatcgccgcg gtcggccagc gctccttctg
     1561 gaagccacga accgtgctgg tgacaggtgc ggggccgctg ctcggcatgt tcctggcgat
     1621 cctcgtcttc agtttcctgg cgaagcgatt cgaagtggcg aaacgatatc atttcgagag
     1681 cggccgtcgc cggatgtttc gcacgatgag ccggcaagat cccgggggac cgtaaccgtt
     1741 tggccagccc ctcctccctc gggaggaact ctctgtgcga tggacacctt gcgccgggtt
     1801 acttcccggc cgggggagcc tttggcgccg ggccggccgc cccggcggtc ggcctgtccg
     1861 cctccgttac ccagccgccg cccatggcct tgtaaaggtt gatcatggcc tggaacagga
     1921 ccccctggtt ctgggtgtag ctcagctgca cgttgaacag cgaccgttgc gcgtcgagca
     1981 cctcgaggta gctggcgtag ccgttgtcga accggagctg ggccagttcc acgtac
//
LOCUS       DATITW010000174         1584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_689742, whole genome shotgun sequence.
ACCESSION   DATITW010000174 DATITW010000000
VERSION     DATITW010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689742"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..201)
                     /locus_tag="VLS86_02390"
     CDS             complement(10..201)
                     /locus_tag="VLS86_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49371.1"
                     /translation="MSRCTGKLHWVQLDAGTNDNDHFIDPEERIRVAMARAVGARTAK
                     EHPRLLAAGPSEGLKRPAI"
     gene            complement(228..713)
                     /locus_tag="VLS86_02395"
     CDS             complement(228..713)
                     /locus_tag="VLS86_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HSO49372.1"
                     /translation="MSIQDGSQTETAPVPSHRTIRKPNPALLEHARERAKSRENRFAD
                     RVTAFSGSMRFVYIHVIWFGCWIGFGLEEYPYGLLTMIVSLEAIFLSTFVLISQNRAD
                     ARRQVVADQQWTMVQEEEAQNIELLDLSRQILKLTQDVHFLTRSRADHGDDRGGPSHS
                     D"
     gene            complement(777..>1584)
                     /locus_tag="VLS86_02400"
     CDS             complement(777..>1584)
                     /locus_tag="VLS86_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSO49373.1"
                     /translation="LGAVQVGMLQALGEHGIAPDLVTGTSIGSINGAVLALDPKGAAN
                     RLSHAWPKMKREHILPGGPIEQARTLRQTKTHLFPNKGLATVISDFLGDDTTFDDLEL
                     PVAAVTMDVATTRPHAIREGRLLPALLASAAIPGVWPPVEHQGRLLYDGGIVANVPLR
                     QAQDMGARSLVVLDCAFAGRLPETPETIAEVLLFTGLVAMRAQAVLEAPLVAATVPVV
                     YLPGPDPHPMSTLDFDHTPELIEASYAAARSFLEHLDITEPGLYGSPSSR"
BASE COUNT          266 a          446 c          551 g          321 t
ORIGIN      
        1 aatcgacaac taaatggccg gacgtttcag cccttcggat ggaccagcgg ccagcagccg
       61 cgggtgctcc ttcgcagtcc gcgctcctac tgcccgcgcc atcgcaaccc ggattcgctc
      121 ctcgggatcg atgaagtgat cgttgtcgtt ggtgccggcg tcgagttgaa cccaatggag
      181 cttgccggtg caccgactca tatgcgcctc gcagatctcg tgcgggatca gtccgagtgt
      241 gatggccccc cgcggtcgtc gccgtgatcc gctcgggagc gtgtgaggaa atgcacgtcc
      301 tgggtcagtt tcagtatctg cctcgagagg tcgagcagct cgatgttctg tgcctcctcc
      361 tcctgaacca tggtccactg ctgatcggcg accacctggc gtctggcgtc ggcgcggttc
      421 tgtgagatca ggacaaaggt ggagaggaag atcgcttcga gcgagacgat cattgtcagg
      481 agaccgtatg ggtattcctc cagtccgaag ccgatccagc agccgaacca gatgacgtgt
      541 atgtagacga agcgcatcga acctgagaag gcagtgaccc ggtccgcgaa cctgttctcc
      601 cttgacttgg cgcgctcccg ggcgtgctct agaagagccg gattcggttt acggatggtc
      661 ctgtggctgg ggacgggggc ggtttccgtt tgtgatccgt cttggattga catcgagtct
      721 cctttcgcca ggcgtcgtcg gtggttgtcg gttccatgcc ggcgaggatg ggtttctcac
      781 ctagacgatg gtgacccgta cagaccgggt tcggtgatgt cgagatgttc gaggaacgat
      841 cgggccgccg cgtaggaggc ctcgatgagc tcaggagtgt gatcgaagtc gagtgttgac
      901 atggggtgtg ggtcgggccc tgggagatac acgacaggga ctgtggcggc aaccaacggc
      961 gcttcgagga ccgcttgagc ccgcatcgcc accagcccgg tgaagagcag cacttcggcg
     1021 attgtctcgg gcgtctctgg gagccggccg gcgaaggcgc agtcgaggac caccagggac
     1081 cgggccccca tgtcctgggc ttgacgcagc ggaacgttgg cgacgatgcc gccgtcgtag
     1141 aggagacggc cttggtgctc gacgggcggc caaactccgg ggatggccgc gctcgccagg
     1201 agcgcgggga gaagcctccc ttctcgaatt gcatgaggtc gggttgtggc gacgtccatg
     1261 gtgaccgcgg caaccggcag ctcgaggtcg tcgaacgtcg tgtcgtcacc gagaaagtcg
     1321 gagatgacgg tggcgaggcc tttgttgggg aataggtggg tcttggtctg cctcagtgtg
     1381 cgcgcctgtt cgatcggtcc gccagggagg atgtgctcgc gtttcatctt cggccatgcg
     1441 tgtgacagcc gattggctgc gcctttgggg tcgagtgcca ggacagcacc attgatcgag
     1501 ccgatggatg tgccggtgac cagatccggg gcgataccgt gttcgcccag tgcctggagc
     1561 atgccgacct ggactgctcc gagg
//
LOCUS       DATITW010000175         1923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_769846, whole genome shotgun sequence.
ACCESSION   DATITW010000175 DATITW010000000
VERSION     DATITW010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769846"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..744
                     /gene="merB"
                     /locus_tag="VLS86_02405"
     CDS             82..744
                     /gene="merB"
                     /locus_tag="VLS86_02405"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HSO49374.1"
                     /translation="MDDEDIRLAIYHSFATTGAPPAYDELASRLGGTYDDVVRGISRL
                     ADQRHVVVDEAGSIVMAHPFASIPLGFSVMGRSTLWWGGCCWDSFAIPHLLTDEPDVL
                     VATRCPGCDGPEAWVVDREGPPDSDWLAHFLVPVSRMWDDVVHTCRNQRLFCSEGCID
                     DWLERGSHQKGYVMDMETLWRLAAGWYQGRLDRGYVRREPVAASEYLRSVGLADPFWG
                     TG"
     gene            complement(778..1764)
                     /locus_tag="VLS86_02410"
     CDS             complement(778..1764)
                     /locus_tag="VLS86_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HSO49375.1"
                     /translation="MIIDAALYRDGVRLPDPFVLEGAHEYCNGTNTFAWIGLYEPTEE
                     EFEAVREEFGLHELAVEDAINAHQRPKLEIYDDTLFVVLKPARYVDSVEVVDFGEILL
                     FIDEDFVVAVRHREASNLVQVRKALETKPQELALGPGAVLHAIVDKVVDDYSDVLAGV
                     EGDIGELEAQVFSSGEAPTERIYKLKREVLQFHQATQPLLEPIERLVSGRFTALSPEL
                     REYFRNSHDHLQRVVERIAAFRDLLTSILEANLIQVTVRQNEDMQRQNEIVKKVSSWA
                     AIIAVPALITGYYGMNVPYPGSGSTWGVVASTLLIAFLSLGRYLVFRRRDWL"
BASE COUNT          359 a          571 c          600 g          393 t
ORIGIN      
        1 tacgatccga ggacatttcg gccggctcac gatctgttcg gcgtggccaa cccggtctcg
       61 gcatggatgt agcttccctc gatggacgac gaagacattc gtctggcgat atatcactct
      121 ttcgcgacga ccggagcgcc acctgcctac gacgagttgg cgtcacgtct cggagggacc
      181 tatgacgacg tcgtccgcgg aatcagccga ctcgctgatc agagacacgt ggttgtcgac
      241 gaggccggga gcatcgtgat ggcccacccc ttcgcctcga tcccccttgg cttctcggtc
      301 atgggccgga gcacactgtg gtggggcggg tgctgctggg attcgttcgc cattccccac
      361 ctgctcaccg acgagccaga cgttctcgtg gctacgaggt gcccgggctg cgatgggcct
      421 gaggcttggg tcgtggatcg ggagggaccg ccggacagcg attggctggc ccacttcctg
      481 gtccctgtgt cccggatgtg ggacgacgtc gtccacacct gccgcaatca gcgtcttttc
      541 tgctccgagg gttgtatcga cgactggctg gagcggggct cgcaccaaaa ggggtatgtg
      601 atggacatgg agaccctatg gcgtcttgcc gccggctggt accaggggcg tctcgaccgt
      661 ggatacgtga gaagggagcc ggtggcggcc agtgagtatc tgcgcagcgt cggtctggcc
      721 gaccctttct ggggcacggg gtgaggacta tccaacggcg acgacagatc gccttcatca
      781 aagccagtcg cggcgtcgga atacgagata gcgacccaaa gaaaggaagg cgatgagaag
      841 ggtcgaggcc acaacgcccc aggtgctgcc cgagccgggg tagggcacat tcatcccgta
      901 gtaaccggtg atcagcgccg gcacggcaat gatcgctgcc cagctgctga ccttcttgac
      961 aatctcattc tggcgttgca tgtcctcgtt ctgccgcacg gtcacctgga tcaagtttgc
     1021 ctccaggatc gaggtgagta ggtcgcggaa ggcggcgatt cgctccacga ctcgctgcaa
     1081 gtggtcgtga gagttgcgga agtactcacg cagctccggg ctgagagcgg tgaagcggcc
     1141 actgaccaac cgctcgatgg gttccagcaa tggttgggtg gcctggtgga attggagcac
     1201 ttctctcttc agcttgtata tgcgctcggt cggcgcctca ccggaggaga acacctgagc
     1261 ctccagttcg ccgatgtctc cttccacacc ggccaacacg tctgagtagt cgtcgaccac
     1321 cttgtcgacg attgcatgga ggacggcacc cggcccgagc gccagctctt gtggtttggt
     1381 ttccagcgcc ttgcgaacct gaaccaggtt ggacgcttcg cggtgacgga cggccacgac
     1441 gaagtcctcg tcgatgaaca gcagaatctc cccgaagtcg accacctcga ccgaatcgac
     1501 gtaccgagcc ggcttgagga caacgaacaa ggtgtcgtcg tatatctcga gcttcggtcg
     1561 ctgatgggca ttgatcgcat cctctaccgc aagttcgtgg aggccgaatt cctctctcac
     1621 agcctcgaac tcctcttctg tgggctcgta gagcccgatc caggcgaagg tgttggttcc
     1681 gttgcagtac tcgtgggcgc cctccagcac aaacggatca ggaaggcgaa ccccatcgcg
     1741 atacagagcg gcgtcgatga tcatgcgtgc caggatacgc ctggtcaact cacatagaac
     1801 caattccctg gagcagggcg ggcttgtcag aaccgctcag tcgaagacgg accagggtgc
     1861 tcttttctgc gctttcctcg ccatggataa ttctcgagat gttgtctatg catttgtaga
     1921 cac
//
LOCUS       DATITW010000176         1605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_209098, whole genome shotgun sequence.
ACCESSION   DATITW010000176 DATITW010000000
VERSION     DATITW010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209098"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VLS86_02415"
     CDS             complement(<1..341)
                     /locus_tag="VLS86_02415"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="lipoyl/octanoyltransferase; catalyzes the transfer
                     of the lipoyl/octanoyl moiety of lipoyl/octanoyl-ACP onto
                     lipoate-dependent enzymes like pyruvate dehydrogenase and
                     the glycine cleavage system H protein; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HSO49376.1"
                     /translation="MSADQGDEGALASTTPLLRARWLGRLPYTEAWDLQRAIHEGKVV
                     GRTGDDYLLLLEHPPVFTIGRNGDASNLLVDREGLAPEVHHVDRGGDITFHGPGQLVG
                     YPILTLDDPKQV"
     gene            complement(338..>1605)
                     /locus_tag="VLS86_02420"
     CDS             complement(338..>1605)
                     /locus_tag="VLS86_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HSO49377.1"
                     /translation="PAAAEQPAAAEQPAAPEQPAAPKQPGATEQPEVEPAAAAAEFAP
                     EPEPVDPQPASTVDAAAASPAEPAEEEVEAFFAEPDETEKKPNLLSPVVRKLIREHEL
                     DVSQLTGTGEGGRITRKDVEAYIESGGPASVTTPAATLPAPATPPPSAPAAAEPVVAE
                     PEPEPEPAPSAPAPAAAAAGSPDVEEVELNRIRIRTAENMIKAKQTAAHVWTSVEVDY
                     ERVERVRQARKEQFKSAEGFSLTYLPFISRAVIDALREYPVVNSSFYLEEKKAVFHKK
                     VDLGVAVDLNQGGLVVARIRDADGLRLVGIARAIRDLAEQARAGQLGVDDLTGSTFTI
                     TNPGPFGSYLSVPIINIPNVAILSTETVSKRATVVTDADGQDMIAIRHIGYLGLTWDH
                     RAFDGSTAVLFLQRIKSNLETWDWEQELA"
BASE COUNT          246 a          542 c          536 g          281 t
ORIGIN      
        1 acctgcttcg ggtcgtccag ggtgaggatc gggtatccga ccaactgccc cgggccatgg
       61 aaggtgatgt caccaccacg atcgacatgg tgcacctcgg gcgccaagcc ctcccggtcc
      121 acgagcaggt tggaggcgtc cccgttcctc ccgatggtga acaccggggg atgctcgagc
      181 agcaggagat agtcgtcccc ggtccgaccg acgacttttc cctcatggat cgcccgctgc
      241 aggtcccagg cctcggtata ggggagccga cccagccagc gggcccgaag tagtggtgtg
      301 gtcgaggcga gagccccctc atcgccctgg tcggcactca cgccagttcc tgctcccagt
      361 cccaggtctc gagattgctc ttgatccgct gcaggaagag gacggcggtg gacccgtcga
      421 aggccctgtg gtcccaggtg agcccgagat acccgatgtg ccggatggcg atcatgtcct
      481 ggccgtcggc atcggtgacc acagtcgctc gcttggagac ggtctccgtc gacaggatcg
      541 caacattggg gatattgatg atcgggaccg agaggtacga cccgaacggc cccgggttgg
      601 tgatggtgaa cgtcgacccg gtcagatcgt cgacgcccag ctgcccggct ctcgcctgct
      661 ccgccaggtc tctgatcgcc cgggcgatcc cgaccagacg caggccgtcg gcgtcacgga
      721 tcctggccac caccagccca ccctggttca ggtcgaccgc gacgccgagg tccactttct
      781 tgtggaacac cgccttcttc tcttccagat agaaggagct gttcacaacc gggtactcgc
      841 gcagcgcatc gatgacggcc cgcgagatga agggcagata ggtcagagag aaaccctcgg
      901 cgctcttgaa ctgctctttg cgggcctgcc gcacccgctc cacgcgctcg tagtccacct
      961 ctaccgaggt ccacacatgc gccgcggtct gcttcgcctt gatcatgttc tccgcggtgc
     1021 ggatccggat ccggttgagc tcgacctctt cgacatccgg ggaaccggcg gcggccgccg
     1081 caggagccgg ggcggacgga gccggttccg gctcgggctc cggttccgca acgacgggct
     1141 cggcggcggc cggagcagac ggaggcggag tggccggggc cggcaacgtg gccgccggcg
     1201 tcgtcaccga agccgggcct cctgactcga tgtaggcctc gacatccttg cgagtgatcc
     1261 gcccaccctc tccggtcccg gtgagttggg acacatcgag ctcgtgctca cggatcagct
     1321 ttcgcaccac cggggacagg agattgggct tcttctccgt ctcgtccggc tcggcgaaga
     1381 aggcctcgac ctcctcctct gccggctctg ccggcgaggc ggccgcggcg tcgacggtcg
     1441 aggcgggttg aggatccacc ggctccggtt ccggggcgaa ctcggccgcg gcggcagcgg
     1501 gctcaacctc tggctgctcg gttgcaccag gttgcttggg cgcagcaggt tgctcaggcg
     1561 cagcaggttg ctcggctgca gcaggttgct cggctgcagc tggct
//
LOCUS       DATITW010000177         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_289245, whole genome shotgun sequence.
ACCESSION   DATITW010000177 DATITW010000000
VERSION     DATITW010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289245"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VLS86_02425"
     CDS             <1..765
                     /locus_tag="VLS86_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HSO49378.1"
                     /translation="PHLPTGIDPFEVLGVVDVGDVYVRPGELEVSIGRIADVVEQISA
                     EGKVPIVLGGDHTITWPDAMGVARVHGFGEIALIHFDAHADTGFIQNGSLHGHGTPMR
                     RLIESGAVPGHRFVQIGLRGYWPEPEVMQWMREQKMRCFLMNEIVDRGLDDVVDEAVE
                     HSLRGGAKGVFISVDIDVVDPGLAPGTGTPEPGGLNSREILDTVRRLARDLNVLGADI
                     VEVSPPYDGPGEQTVYLANRVVLEVLNGMAERKLAT"
     gene            complement(804..1193)
                     /locus_tag="VLS86_02430"
     CDS             complement(804..1193)
                     /locus_tag="VLS86_02430"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HSO49379.1"
                     /translation="MTQDPNPVTSDQQTWRVLAHASAFIQVIGVPSLVGPLVVWLMKR
                     DDPVVEPHARAALNFQLTLLIYFVGGIVLAFVAAITIVGLVLTVLIVVFLVLLILLEL
                     VFALLASLAASRGELYVYPMSLELIKP"
     gene            complement(1234..1860)
                     /locus_tag="VLS86_02435"
     CDS             complement(1234..1860)
                     /locus_tag="VLS86_02435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSO49380.1"
                     /translation="MSEVTIVNLERSMAAELEQIELTCFPMANPRDLLSREDIEAYAG
                     TFPQGYFVAIVDGRPVGMGAGIYLDFDFERPQHTIASITGDHQCGNHDPEGDWYYGTD
                     ITVLPEYRGRGIGGMLYEKRKALVVRDGKRGIIAGGSLPGYFHHKSSMPIQEYVDRVV
                     AGELTDPTLSFQMSHGFEVRGLLENYLEDEADDGWAVLIVWENRQAEG"
     gene            complement(1857..>2219)
                     /locus_tag="VLS86_02440"
     CDS             complement(1857..>2219)
                     /locus_tag="VLS86_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49381.1"
                     /translation="LLIGPDGSGVNIEAMPGSVKVDRVTEHPFVHTNHCIDDATRLEE
                     GTRTDEHVVGSQTRLQRGSALAEDLDAFFSEPSIARRSTSPSDVATCGAVVMRSRERR
                     LDSVWGIPGDVPWETFAL"
BASE COUNT          397 a          722 c          713 g          387 t
ORIGIN      
        1 ccgcatcttc ccaccggaat cgaccccttc gaggtgctgg gggtggtcga cgtgggagac
       61 gtctacgttc ggcccggcga gctcgaggtc tccatcgggc ggatcgccga cgtcgtggag
      121 cagatctcgg ctgaggggaa ggtcccgatc gtcctcggcg gtgaccacac catcacctgg
      181 ccggacgcga tgggagtggc tcgggttcat gggtttggcg agattgccct gatccacttc
      241 gacgctcacg ccgacaccgg cttcatccag aacggctcgc tccacggtca cgggacacca
      301 atgcgccggc tcatcgagtc gggtgccgtg ccggggcatc gcttcgtcca gatcggactt
      361 cgcggttatt ggccggagcc ggaggtgatg cagtggatgc gggagcagaa gatgcgctgt
      421 ttcctcatga acgagatcgt ggaccgcggg ctggacgatg tcgtcgacga ggcggtcgag
      481 cactcgctga gaggtggggc gaagggtgtg ttcatcagcg tcgacatcga cgtcgtcgac
      541 cccgggctcg cacccggcac cgggacccca gagcccggag ggctcaactc gcgggagatc
      601 ctggacaccg tgcgccggct cgcccgcgat ctcaacgtgc tcggcgccga catcgtggaa
      661 gtctccccgc cgtatgacgg tcccggtgag cagacggtct atctggccaa cagagtcgtc
      721 ctcgaggtcc tcaacggcat ggcggagcga aagctcgcca cctgacccct gaggcccggt
      781 cgagcccgag cccgatcagg cgatcaaggc ttgatcagct ccaagctcat cgggtagacg
      841 tacagctcgc ccctcgaggc ggccagcgac gccagcaggg caaataccag ctcgagcagg
      901 atcaacaaga cgaggaagac cacgattagg acggtcaaca ccagacccac gatcgtgatc
      961 gcggccacga aggccaggac gattccgccc acgaagtaga tcagaagagt cagttggaag
     1021 ttgagcgcgg ccctggcgtg tggctccacc accgggtcgt cccgtttcat caaccagacc
     1081 acgagtggcc cgaccaggga cgggacgccg atgacctgga tgaaggccga ggcgtgggcg
     1141 agaacccgcc acgtctgctg atcgctggtg accgggtttg gatcctgtgt catgcctcaa
     1201 tggtaggtgc gagggtgctt ccggcacccg taatcatcct tcggcctgcc gattctccca
     1261 gacgatcaag acggcccaac cgtcgtccgc ttcgtcctcg aggtagttct ccaacagacc
     1321 cctcacctcg aagccatggg acatctggaa gctgagcgtg ggatcggtca actctcctgc
     1381 caccacccgg tcgacgtatt cctggatcgg catcgaagac ttgtggtgga aatagccggg
     1441 gagagagcct ccggcgatga ttccccgctt gccgtcgcgt accaccagcg ccttgcgctt
     1501 ctcgtagagc atccccccga tcccgcggcc gcggtattcg ggcagcacgg tgatgtcggt
     1561 cccgtagtac cagtcgcctt cggggtcgtg gttcccgcac tgatggtcgc ccgtgatgct
     1621 ggcgatcgtg tgttgtggtc gctcgaaatc gaagtcgaga tagatgccgg cccccatccc
     1681 caccgggcgg ccgtcgacta tggcaacgaa gtaaccctgg ggaaaggtgc cggcatatgc
     1741 ctcgatgtcc tccctggaga ggagatccct cgggttcgcc atcgggaagc aggtcagctc
     1801 gatctgctcc aactcggcgg ccatgctccg ctcgaggttg acgattgtca cctcgctcac
     1861 agagcgaagg tctcccaggg aacgtctccc gggatgcccc acaccgagtc gagccgtctc
     1921 tccctggacc gcatcaccac cgcgccgcag gtcgccacgt cgctcggcga agtggaacgt
     1981 cgggcgatcg acggctcgga gaagaaggca tcgagatcct ccgccagcgc cgagcctcgc
     2041 tggagacggg tctggctgcc aaccacgtgc tcgtcggtcc tcgtcccctc ctcgagccgg
     2101 gtggcgtcgt cgatgcaatg gttggtgtgg acgaaggggt gctcggtcac tcgatccact
     2161 ttcaccgacc ccggcatcgc ctcgatgttc accccggacc catcggggcc gatcagcag
//
LOCUS       DATITW010000178         1808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_8952, whole genome shotgun sequence.
ACCESSION   DATITW010000178 DATITW010000000
VERSION     DATITW010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8952"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VLS86_02445"
     CDS             <1..310
                     /locus_tag="VLS86_02445"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSO49382.1"
                     /translation="WRTVVTTPSRVRFPEGESLLEAQTRMVKTCQDLANRHRNQAIVA
                     VSHADTIKAALSHYLGQPLDLFNRIAVAPASVAVIDLPESGAPRVVTMNSNGDPQTWQ
                     "
     gene            318..809
                     /locus_tag="VLS86_02450"
     CDS             318..809
                     /locus_tag="VLS86_02450"
                     /inference="COORDINATES: protein motif:HMM:NF022734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HSO49383.1"
                     /translation="MALEDLGPAERLAAGAIGQPGQRRFFLEVTAGGRIHSFPCEKSQ
                     VSELAQQGLQLLGLASLDSDEEAVQRLVLSRLPIIDPEEPKFRISSIGVAVHESEFLS
                     ITLSSPDDEDSATVVVTPEQFRAMALIAVEVVAAGRPICPRCGLPEDPDGHRCPSVNG
                     HHG"
     gene            1000..1494
                     /locus_tag="VLS86_02455"
     CDS             1000..1494
                     /locus_tag="VLS86_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49384.1"
                     /translation="MGLDLVPETILADGPFGPGSAQRFVEEDFDFDPRPLFIPQLDRR
                     LWPVAVLDIVCNNADRKIGHVIHDKTTDRLWAIDHGLTFHADPKLRTVLWGFAGEPIP
                     APLLEALGGLRARLTSGLSTRVAELLSEEEAVSLSARVEELISARVHPHPPADRPAVP
                     WPLV"
     gene            complement(1513..>1808)
                     /locus_tag="VLS86_02460"
     CDS             complement(1513..>1808)
                     /locus_tag="VLS86_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49385.1"
                     /translation="LRMFVEAGDLSALPLHVADFVQLALARDQFDRAIVLAGAASALQ
                     TYSETRLLDLVANDVKGLERAIEGVGRERAEKLAAEGQALSVDQILDRIATDE"
BASE COUNT          326 a          581 c          560 g          341 t
ORIGIN      
        1 ctggcgtaca gtcgtgacca ctccatcgcg ggtcagattc cccgagggcg aatcccttct
       61 cgaagcacag acaaggatgg tcaagacctg ccaggacctg gccaaccgcc atcgcaacca
      121 ggcgatcgtg gcggtgagtc atgccgacac catcaaggcg gcgttgtccc attacctcgg
      181 tcagcccctt gacttgttca atcggattgc ggtagcaccc gcctcagtgg ccgtgatcga
      241 tcttcccgaa agcggggcac cccgggtcgt caccatgaac agcaacggag atccccaaac
      301 atggcaatag aagagacatg gcattagaag atttgggacc ggcggagcga ttggccgcag
      361 gcgccatcgg acagccgggg cagcggcgct tcttccttga agtgactgca ggcggccgaa
      421 ttcactcctt cccatgtgag aagagccagg tttccgagct cgcccagcag gggcttcagt
      481 tgctcggtct ggccagtctc gactcagacg aagaagccgt gcaaaggctg gtcctcagtc
      541 gcctgccgat catcgacccc gaggagccca agttcaggat cagctcgatc ggggtggcag
      601 tgcacgagag cgagttcctt tcgatcacac tctcctcccc cgacgacgag gactccgcga
      661 ctgttgtcgt cactcccgaa cagttccggg ccatggctct gatagcggtc gaggtggtgg
      721 cggccggtcg accgatctgt cctcgctgcg gtttgcccga ggaccccgac ggccaccggt
      781 gtccttcggt caatggccat catgggtgag gacggccgtt tggttctggt cgaggtcgag
      841 ggccagatgc cgtacgccag caacacgacc cttctcgcca aagatgcctc cggcgacttg
      901 tgggtctaca agcccgagcg tggtgagaac ccactctggg acttcgcctg gcgcacgctg
      961 gcagcgcgtg aggttctgac ctatgaggtc gctaccgggt tgggcctcga tctcgtgccg
     1021 gaaacgatcc tggccgatgg accgttcgga ccgggttcgg cccagcggtt cgtcgaagaa
     1081 gatttcgact tcgatccgcg gccgctgttc atcccccagc tcgatcggcg cttatggccg
     1141 gtggcggtgc tagacatcgt ctgcaacaac gccgaccgca agattggcca cgtcattcac
     1201 gacaagacga ccgatcggtt gtgggccatc gatcacggcc tcacctttca cgccgatccg
     1261 aagctgcgaa ccgtcctgtg ggggttcgcg ggcgagccaa tcccggcgcc gctgctggag
     1321 gctctgggcg gactgcgggc gcggttgacg tccggcctct cgacgcgggt ggccgagctg
     1381 ctgtcggagg aagaagctgt cagcctgtcc gctcgagtcg aggagctaat cagcgcacgg
     1441 gttcatcctc atccacccgc cgatcgtccg gccgttccct ggcccctggt ctagtcgacg
     1501 tcgctgccag gatcattcgt cagttgcgat tcgatccagg atctggtcca cgctcaacgc
     1561 ctggccttcg gccgctagct tctcagcgcg ctcgcgcccg acgccttcaa ttgcacgctc
     1621 aagtcccttg acgtcgttgg cgaccagatc gagcaaccgg gtctcgctat aagtctgcag
     1681 tgcgctggcc gcaccggcca ggacgatggc ccggtcgaat tgatcacgcg ccagggccag
     1741 ttgaacgaag tcggcgacat gaagtggcag ggctgagaga tcccctgcct cgacgaacat
     1801 ccgtagac
//
LOCUS       DATITW010000179         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_329339, whole genome shotgun sequence.
ACCESSION   DATITW010000179 DATITW010000000
VERSION     DATITW010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329339"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /gene="lnt"
                     /locus_tag="VLS86_02465"
     CDS             complement(<1..1253)
                     /gene="lnt"
                     /locus_tag="VLS86_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HSO49386.1"
                     /translation="MIGAVLVVSGAILSALAFPRFGPGWLILPGIGLFLAGLRTAAGR
                     AQGLILGALYGLTFFACLIWWIGELGLIALIPLVLVQAAFPTAYGWWLARYNERTPGT
                     WFLLAVGGWALMELLRYHFPFNGLEWGAAGYALSDQGWARGLAPLYGTTGITILVVMS
                     AATMVALSSRPRNWWLLVPVPALVIGYWVWSFQNETPVLEGRSVAVVQGSTPCPFEHC
                     PPDERLRTFEQHLKLTRTIEPGSVSLVVWSEGSTGSFNADPVQNPESGAAIGAEARRI
                     GAWFVVGSDRPINETHWVNANVVFDPEGQIVGEYRKQHPVPFGEYIPFRPLFEWIPDL
                     DQVPRDMIPGEGPVVFDLGGGLQMGTVISFEGGFGRYPRQHVRAGANLIVVATNEASY
                     GTTPGSDQFIGMTRMRAAELNLDLV"
     gene            1303..2109
                     /locus_tag="VLS86_02470"
     CDS             1303..2109
                     /locus_tag="VLS86_02470"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSO49387.1"
                     /translation="MWEGDVWVAVRAARAGAEVVRHGFLDSFTTEMKGVVDPVTEVDR
                     ESEEAVKAVIATHFPDDAILAEEGGGADWRGRRVWIVDPLDGTVNFVNRIPQVAVSVA
                     LWSEGKPLVGVVVDVSRDEEFVAAHGEGAHCNGEPIRVSDTSVLEDSLLVTGFPYDRQ
                     EHARAYLDVMEQVMMRTRGTRRMGAAALDMAWVACGRFDGYWEHGGPAGVKPWDAAAG
                     TLLVTEAGGMATDSKGGVFDLEPSAFVVTNGKIHEDLRQIVDDYMPEHLR"
     gene            2109..>2558
                     /locus_tag="VLS86_02475"
     CDS             2109..>2558
                     /locus_tag="VLS86_02475"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSO49388.1"
                     /translation="MYHDHHFHPLGYANLVTGLELMDAADLGEVMRRVVARAEQVSGP
                     IIGQRLNDEGLAERRLPTRVDLDEAVSDRPVLLYRYCGHIAVANTKALDLAGVDARSS
                     DPIGGSIDRDATGAPTGVLRETGITMVSRALAQHVAVPSDAEILRALN"
BASE COUNT          471 a          838 c          868 g          381 t
ORIGIN      
        1 tggaccaggt ccaggttcag ctcggcagcg cgcatccggg tcatgccgat gaactgatca
       61 gaaccggggg tggtcccata ggaggcctcg ttggtggcca ccacgatgag attggcgccg
      121 gcccgcacgt gttgtcgcgg ataacggcca aagcctccct cgaacgagat caccgtcccc
      181 atctgcagac cgccaccgag gtcgaagacc accgggccct cgccagggat catgtcgcgg
      241 ggaacctggt cgaggtccgg gatccactcg aagaggggac ggaacgggat gtactccccg
      301 aacgggaccg gatgctgctt gcggtactcg ccgacgatct ggccctcggg gtcgaacacc
      361 acattggcgt tgacccagtg ggtctcgttg atcgggcggt cgctacccac cacgaaccag
      421 gccccgatgc gccgggcctc ggccccgatc gcagccccgc tctcggggtt ctgcaccgga
      481 tcggcattga acgagccggt tgacccttcg gaccagacca ccagggacac cgagccgggc
      541 tcgatggtgc gggtcagttt gagatgctgc tcgaaggtcc gcagccgctc atccgggggg
      601 cagtgctcga aggggcaggg cgtcgagccc tggaccacag ccaccgatct cccctcgagc
      661 accggtgtct cgttctggaa ggaccaaacc cagtacccga tcaccagtgc cggcaccggc
      721 accaacagcc accagttccg gggcctggag ctcagggcca ccatcgtggc tgcagacatc
      781 accaccagga tcgtgatccc ggtggtcccg tacaaaggag ccaagccacg cgcccagcct
      841 tggtcggaaa gtgcgtatcc cgcggccccc cactccagcc cgttgaacgg gaagtggtat
      901 cggagcaact ccatcaatgc ccacccccct accgcgagca ggaaccaggt gcctggagtg
      961 cgctcgttgt atctcgccag ccaccacccg tacgccgtgg gaaaggcggc ctggaccagg
     1021 accaagggga tgagcgcgat cagacccagt tcgccgatcc accagatcag gcaggcgaag
     1081 aaggtgagac cgtacagagc gccgagaatc agcccctgcg cccggccggc cgcggtgcgc
     1141 aacccggcga ggaagagccc gatcccgggc aggatcagcc atccgggccc aaatcgagga
     1201 aaggccaggg cagacaggat tgcccccgac accaccaaca cggcaccgat catcgggggc
     1261 agggtagaaa gccctcgccg aaaccaccgt agagtgactg cgatgtggga aggtgacgtg
     1321 tgggttgcgg tgcgtgcggc gagagccggc gccgaagtcg tgcgtcatgg ttttctcgac
     1381 agcttcacca cggagatgaa gggggtggtc gacccggtca ccgaggtcga ccgcgagtcg
     1441 gaggaggcgg tcaaggccgt catcgccact cacttccccg acgatgcgat cctcgccgag
     1501 gagggtggcg gggccgattg gagagggcgc cgggtctgga tcgtcgatcc gctcgacggt
     1561 acggtcaact tcgtgaatcg gatcccacag gtggcggtgt cggtcgccct gtggagcgag
     1621 ggaaagccct tggttggggt ggtggtagac gtctcccgag acgaggagtt cgttgccgcc
     1681 cacggcgagg gagcgcactg caacggtgag ccgattcggg tctcggacac gtcggtgctg
     1741 gaggactcac ttctcgtcac cgggtttccc tatgaccgcc aggagcacgc tcgcgcctac
     1801 ctcgacgtca tggagcaggt gatgatgcgg acccggggca cgagacggat gggcgccgcc
     1861 gccctggaca tggcctgggt ggcctgtggc cgattcgacg ggtactggga gcacggaggg
     1921 ccggccgggg tcaagccatg ggacgccgcc gccgggaccc tcctcgtgac cgaggccggg
     1981 ggaatggcca ccgacagcaa gggaggggtg ttcgacctgg agccatcggc cttcgtcgtc
     2041 accaacggca agatccacga ggacctccgc cagatcgtcg acgactacat gcccgagcat
     2101 ttgcgttgat gtatcacgac caccatttcc accccctcgg gtacgcgaac ctggtcaccg
     2161 ggctcgaatt gatggacgct gccgacctcg gcgaggtaat gcgccgggtc gttgccaggg
     2221 ccgagcaggt gagcggcccg atcatcggcc agcgtctgaa cgacgagggt ctcgccgaga
     2281 ggaggcttcc gacccgtgtc gatctggacg aggcggtcag cgatcgcccg gtgctcctct
     2341 atcgctactg cgggcacatc gcggtggcca acaccaaggc cctggatctt gccggggtcg
     2401 atgccaggtc ctcggacccg atcggcggct ccatcgaccg cgacgccacc ggcgcaccca
     2461 ccggggtgtt gcgggaaacc gggatcacga tggtgtcgag agcgctggcg cagcacgtgg
     2521 ccgtcccctc cgacgccgag atcctccgcg ccctgaac
//
LOCUS       DATITW010000180         1594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_128993, whole genome shotgun sequence.
ACCESSION   DATITW010000180 DATITW010000000
VERSION     DATITW010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128993"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VLS86_02480"
     CDS             complement(<1..556)
                     /locus_tag="VLS86_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HSO49389.1"
                     /translation="MAERDPRILLLTGDLGYTVLEPFAERFPDRFFNVGVAEQNMVGV
                     ATGLAEAGFLPFCYSIATFISLRSYEFIRNGPILQRLPVRIVGVGGGFEYGPAGATHH
                     ALEDVGIMRLQPGLTVIAPADHEQARTAVLATWKRPGPIYYRLGKDDRTAVTGLGGRF
                     ELGRIQLIREGAHLLIITMGSIASE"
     gene            complement(586..1398)
                     /locus_tag="VLS86_02485"
     CDS             complement(586..1398)
                     /locus_tag="VLS86_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HSO49390.1"
                     /translation="MEAVEGLPLSSRQIRKIVLDQSKRAHVGHIGSALSIAEIIAALY
                     AHVLRIPDPGDPDRDRFVLSKGHAVLAVYAALHLKGWLTEAELNTFCADGSLLGVHPE
                     HALRGVDFSTGSLGHGLSMGAGAALAARLRHSRRRVFVLVSDAECNEGSLWEAVMFAA
                     HHRLANLIAIIDLNGQQAFGYTDQVLSLTPMAERWRAFGWDVREINGHDVAAITETIA
                     GLETASGPPHVVIAHTVFGKGVSYMERQIKWHYWPMSDAEYRQALDEIDATP"
BASE COUNT          288 a          538 c          484 g          284 t
ORIGIN      
        1 cctcgctcgc gatgctcccc atcgtgatga tcagcaggtg ggcaccttct cggatgagtt
       61 ggatgcgtcc gagttcaaat cgcccgccaa gaccggtcac cgcagtccgg tcatcctttc
      121 cgagccggta atagatcgga ccgggccgct tccaggtcgc caggaccgct gtgcgggcct
      181 gttcgtggtc ggccggggcg atcaccgtca ggcccggctg caggcgcatg atgcccacgt
      241 cctccaaggc gtggtgggtc gcgcccgcag ggccatactc aaatccgccg cccactccga
      301 cgatccgcac cggcaaccgc tgcaggatgg ggccgttccg aatgaattca taggaccgca
      361 gcgagatgaa ggtggcgatc gaatagcaga acgggaggaa gcccgcttcg gccagtccgg
      421 tcgccacccc gaccatgttc tgctctgcca cccccacgtt gaaaaagcga tccgggaatc
      481 tctccgcgaa cggttccagg accgtgtagc ccagatcgcc cgtgaggagc agaatgcgcg
      541 gatcccgctc agccaattct accagggttc tcgtgaatgc tcctctcatg gcgtcgcgtc
      601 gatctcgtcc agcgcctggc ggtattccgc gtccgacatg ggccagtagt gccacttgat
      661 ctggcgctcc atgtaggaga cccccttgcc gaacacggtg tgggcgatca ccacgtgggg
      721 cgggccggag gcggtctcca gcccggcgat cgtctcggtg atggccgcga cgtcatgccc
      781 gttgatttcc cgcacatccc agccgaacgc ccgccagcgc tcggccatcg gggtgaggga
      841 taacacctgg tccgtatagc cgaacgcctg ctgtccgttc aggtcaatga tggcgatcag
      901 attcgccagg cggtgatgtg cggcaaacat gacggcctcc cagagggacc cctcgttaca
      961 ctctgcgtcg ctcaccagca cgaacacgcg ccgccgggaa tgtcgcaggc gtgccgccag
     1021 tgcggcgcct gcccccatgg acaaaccatg gccgagtgag ccggtcgaaa agtccacgcc
     1081 cctgagcgca tgctccggat ggacccccaa caggctgcca tctgcgcaga aggtattcag
     1141 ctccgcttcc gtcagccacc ccttcaggtg cagcgccgcg tagaccgcga ggacggcgtg
     1201 tcccttggac agcacgaacc ggtcgcgatc gggatcgccc gggtcgggaa tgcgcaggac
     1261 gtgcgcatac agcgctgcaa tgatctcggc gattgacagg gcagagccga tatgtcccac
     1321 atgcgcccgt ttcgactggt cgagcacgat ctttctaatc tgacgagacg agagtggtag
     1381 tccctccacg gcttccatct ccaactagat cctccctcgc ttcccacact cgcagcggtt
     1441 cgggtctcca cagggtgggt tccgcgggac cggggaattt cctgagcagg cgccactgtt
     1501 cggggtcaac ttcgtcggcc cggggaatgc aatcaatgca cccgtaatac ggctccctgc
     1561 ctcctcccgt aacaaggtac ccggacttga tggc
//
LOCUS       DATITW010000181         2402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_529738, whole genome shotgun sequence.
ACCESSION   DATITW010000181 DATITW010000000
VERSION     DATITW010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529738"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VLS86_02490"
     CDS             <1..232
                     /locus_tag="VLS86_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HSO49391.1"
                     /translation="TREVFEILEHTKPGHGGEIQLTDAIKELGRRRRLRGYVAADDLL
                     DVGTPLGLLEASVELGEHQFGDEFGAWLRARR"
     gene            211..1458
                     /gene="glp"
                     /locus_tag="VLS86_02495"
     CDS             211..1458
                     /gene="glp"
                     /locus_tag="VLS86_02495"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HSO49392.1"
                     /translation="MAESQEMKPLEEARSEVLSTVRVLGSETVPIWEALGRILAADVI
                     APEDVPPFPNSAMDGFAVRAEDVSSPGAVLEVLGDLQAGYTTETEVGPGEAIKIMTGA
                     PIPSGADTVVRVEDTAAEGSKVEIAVAVESGTSVRPAGGDVKRDQLVFEAGTRLTPMH
                     IGVLATNGGAKPEVFKRPRVAYMSTGDELAPVETPALVPGGIRDSNRPMLRALLEEAG
                     AELIDLGIVPDDTDVLRGALDSGSQADVMVSTGGVSMGDYDVTKLVLVGDAGVEFWKI
                     AIQPAKPFAFGRVGAALFFGLPGNPVSVLVSFEQFLRPALLRMQGARAVLRPQMVAVA
                     GEDLDTDPEKTVFVRVLVEGLAEGVPRVIKSGGQSSNVLSAAAAADAFAVVPEGVAAV
                     RSGEPVTIEMFKWPEAREWADGR"
     gene            1448..1927
                     /gene="moaC"
                     /locus_tag="VLS86_02500"
     CDS             1448..1927
                     /gene="moaC"
                     /locus_tag="VLS86_02500"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HSO49393.1"
                     /translation="MADDQGLPHLNEAGEAHMVDIGDKAVTDREAVAEGVVTMSPELV
                     ERFFAGDLPKGDAAAVARIAGITAAKKTSDLIPLCHPISLTGVEVSLTRSGRGIRVLA
                     TVRTTGRTGVEMEAMTAVAVAALTIYDMVKGVERGVTVESIGLLRKSGGRSGTWKRT"
     gene            1991..>2402
                     /locus_tag="VLS86_02505"
     CDS             1991..>2402
                     /locus_tag="VLS86_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49394.1"
                     /translation="METSVIVGLLLIAAVWSVYLLPSVFGDRQNAPLNSTEEFDRWTH
                     LMADVQKRPYSRNAVGERDIIRLRRRRTIAVLATFALVTAGLALLQNSLPLLLLHLFV
                     DSLIALYVAALAQMKQQRQWRLKVTHVSERPAEWD"
BASE COUNT          398 a          742 c          848 g          414 t
ORIGIN      
        1 cacgcgggag gtgttcgaga tcctggagca caccaagccg ggccatggcg gtgagatcca
       61 gctgacggat gcgatcaaag agctggggcg acgacggcgg ctgcgcgggt atgtggcggc
      121 cgacgacttg ctcgacgtcg ggaccccgct cgggctgctc gaggcatccg tcgagctggg
      181 cgagcaccag ttcggcgacg agttcggcgc atggctgaga gccaggagat gaagcctcta
      241 gaggaggcca ggtccgaggt cctgtccacg gtgcgtgtcc tcggatcgga gacggttccc
      301 atctgggagg cgctgggccg gatcctcgcc gctgacgtca tcgcccccga ggacgtgcct
      361 ccattcccca actcggcgat ggacggattc gcggtccggg ccgaagacgt ctcgtctccg
      421 ggggcggtcc tcgaggtgct tggtgacctc caggcggggt acacgaccga gaccgaagtg
      481 ggacccggcg aggcgatcaa gatcatgaca ggggcgccaa tccccagtgg ggccgacacg
      541 gtggtcaggg tcgaggacac ggcggccgaa ggatcgaagg tggagatcgc ggtcgctgtc
      601 gagtcgggca cctcggtgcg gccggcaggc ggggacgtca agcgcgatca gctcgtgttc
      661 gaggccggca cccggctcac ccccatgcat atcggggtcc tggccacgaa cgggggcgcc
      721 aagccggaag tattcaagcg tccccgtgtt gcctacatgt ccaccggcga cgagctcgca
      781 cccgtggaga ccccggccct ggtcccgggc gggatccgag actcgaaccg ccccatgctc
      841 agggcgctcc tcgaggaggc cggcgccgag ctgatcgacc tcggcatcgt gcccgacgat
      901 accgacgtcc tgcggggcgc cctcgacagt ggttcccaag ccgacgtcat ggtgtcgacc
      961 ggcggagtct cgatgggtga ctacgacgtg accaagctgg ttctggtagg cgacgccggg
     1021 gtggagttct ggaagatcgc catccagccg gccaagccgt ttgcctttgg gcgtgtcggc
     1081 gccgccctgt tcttcggtct ccccgggaac ccggtgtcgg tgctggtttc cttcgagcag
     1141 ttcctccggc ccgccctgct ccggatgcag ggtgcgcgcg ccgtgctccg accccagatg
     1201 gtcgcggtgg ccggcgaaga cctcgacacc gacccggaga agacagtgtt cgtcagggtc
     1261 ctcgtcgagg gtctcgccga gggtgtcccc agggtgatca agtcgggcgg ccagtcgtca
     1321 aacgtgttgt ctgcggcggc ggcggccgac gcctttgccg tggtgcccga gggcgtcgcg
     1381 gcggtcagga gtggtgagcc ggtgaccatc gagatgttca agtggccgga ggcgagggag
     1441 tgggccgatg gccgatgatc agggtctgcc ccatctcaac gaagcggggg aggcccacat
     1501 ggtcgacatc ggcgacaagg cggtcaccga tcgggaagcg gtcgccgaag gagtcgtgac
     1561 aatgtcgccc gagctggtgg agcggttctt cgccggcgac ctgcccaagg gagacgcggc
     1621 tgccgtggcc aggatcgcag gcatcaccgc ggcgaagaaa acctcggatt tgatccccct
     1681 gtgtcatccg atctccctca ccggggtgga agtctcgctc acgcggagtg ggcgtgggat
     1741 tcgagtgctt gctaccgttc gcactaccgg gaggaccggt gtcgagatgg aggccatgac
     1801 cgccgtcgcc gtggccgctc tgaccatcta cgacatggtg aagggtgttg agcgcggagt
     1861 cactgtggag tcgatcggac tgttgaggaa gtctggtgga aggtcaggca cctggaagag
     1921 aacttaggtc gttgatcggg cgtggggtct gccgctacgc tcgtacatgg acggagtagg
     1981 cgaaagctaa atggaaacaa gcgtcatcgt cggcttactg ctgatagccg cggtatggag
     2041 tgtttacctg ctgccttcgg ttttcgggga cagacagaac gctcccctga actcgaccga
     2101 agagttcgat cgctggaccc acctgatggc cgacgtccag aagcgcccct acagccgcaa
     2161 cgcggtcggc gagcgggaca tcattcggct tcgccgacgc aggaccatcg ccgttctcgc
     2221 caccttcgcc ctggtcaccg ccggtcttgc gctcttgcag aactcactgc cgctgctctt
     2281 gttgcatctc ttcgtcgact ccctgatcgc cctctatgtg gccgccctgg cccagatgaa
     2341 acagcagcgc caatggaggc tcaaggtcac ccatgtctcc gagcgccctg cggagtggga
     2401 cg
//
LOCUS       DATITW010000182         1938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_369414, whole genome shotgun sequence.
ACCESSION   DATITW010000182 DATITW010000000
VERSION     DATITW010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369414"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..726)
                     /locus_tag="VLS86_02510"
     CDS             complement(70..726)
                     /locus_tag="VLS86_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSO49395.1"
                     /translation="MTERSKPRIFLVEDHPLTLFGLQRYLEDPFDVVGSATEAGAAIE
                     MILERRPDLVLLDVHFPGGGGAAVIEAVKKVDSEIKFLAFTVSTSAEDVVRLFKAGVD
                     GYVVKTTDAAALAEQIELALDGGRPVSRYVAGHLLSIDEVVSTHSEIESLTPKEREVV
                     TLIARGFRYREAAEELGISQKTLETHMRHVFEKLGVASRHEMTRMAFETGFLRPGDFD
                     "
     gene            complement(723..1865)
                     /locus_tag="VLS86_02515"
     CDS             complement(723..1865)
                     /locus_tag="VLS86_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSO49396.1"
                     /translation="MTEASHHDSRLLPVDRTLTRVVVLMRLLGWAWMVILVVVAMLTA
                     FPEPDTRILAGALVLGTAGTGLTLLAASRGFLGEPWYVVVDGFLALLLVSAGWFAGTD
                     FVSGGYPASWLFIVAFATNFRATSVIGVVATAVFALLHLGMALDQTRLFGSVQFLVVA
                     VVVGWAFDALRERERLRITAETERAEAEAELARERERSMRLEERSQIARRLHDSVLQT
                     LKLISDTADDSAEVRYLARVQERDLRRTINEYQSPHQDSFRARLLDVQANVEDRFRVE
                     IDHVIRDDAEMDDALHAVVAATAEALNNAARHSGAHQIDLYSEIRDGVAQVNVRDRGT
                     GFDPETVARGGLVDSIVSRVTAAGGSAEVRSAPGKGTEVVIRAPVS"
BASE COUNT          352 a          674 c          601 g          311 t
ORIGIN      
        1 gtatgtgctc atgaggacag catcggtcat ggacaggaga acggccatcg ggatataccc
       61 cgatgccagt cagtcgaagt cgccggggcg gaggaagccg gtctcgaacg ccatccgggt
      121 catctcatgg cggctggcca cacccagctt ctcgaagacg tgcctcatgt gggtctccag
      181 cgtcttctga ctgatcccaa gctcctcggc cgcctcccga taacggaaac ccctggcgat
      241 cagcgtgacc acctcccgct ccttcggagt caggctctcg atctcgctgt gagtcgaaac
      301 cacctcgtcg atgctgagca agtgcccggc cacgtaccgg gacaccgggc gcccaccgtc
      361 gagggccagt tcgatctgct ctgccagtgc ggcagcgtcc gtggtcttga ccacatagcc
      421 gtccaccccg gccttgaaca acctgaccac gtcctcggcc gaggtggaga cggtgaaggc
      481 gaggaacttg atctcgctgt cgaccttttt caccgcttcg atcaccgcgg cgcctccgcc
      541 gccggggaaa tggacatcga gcaggaccag gtcgggtcgg cgctcgagga tcatctcgat
      601 ggcggctcca gcctcggtcg ccgagcccac cacgtcgaaa ggatcctcca gatatcgctg
      661 caagccgaac aaggtcagcg ggtggtcttc gaccaggaag atccgaggct tcgatcgctc
      721 cgtcacgaca cgggggcccg gatcaccact tcggttccct tgcccggggc cgacctcacc
      781 tcggcgctgc ccccggcggc tgtcacccgg ctgacgatcg aatcgacaag cccgccgcgt
      841 gccacggtct ccgggtcgaa cccggtcccg cggtcgcgga cattgacctg ggccaccccg
      901 tcacgaatct cggagtacag gtcgatttgg tgggccccgg agtgacgggc cgcgttgttc
      961 aacgcctccg cggtggcggc gaccaccgcg tgcagtgcgt cgtccatctc ggcgtcgtcg
     1021 cggatcacat ggtcgatctc gacccgaaac cggtcttcga cgttggcctg cacgtcgaga
     1081 agccgtgccc ggaaactgtc ctggtgcggc gactggtact cgttgatcgt ccgtctcaga
     1141 tctcgttcct ggacccgggc caggtagcga acctcggcgg aatcgtcggc ggtgtcgctg
     1201 atcaacttca acgtctgcaa caccgaatcg tggagccggc gggcgatctg cgatctctcc
     1261 tcgagccgca tcgaccgttc ccgctcccgg gccagctcgg cctccgcctc cgcccgctcc
     1321 gtctcggccg tgatccgaag cctttctctc tcgcgcaggg cgtcgaaggc ccaaccgact
     1381 accaccgcca ccaccaggaa ctggacgctt ccgaacagcc gggtctggtc gagcgccatc
     1441 cccaggtgga gaagggcaaa tactgccgtg gccaccaccc cgatgacact cgtcgcccgg
     1501 aagttggtgg cgaaggcgac gatgaacagc catgaggctg ggtagccgcc cgagacgaaa
     1561 tcagtcccgg cgaaccaccc tgcggagacg aggagcagag ccagaaaacc gtcgacgacc
     1621 acgtaccagg gctcaccgag gaatccccgc gaggcggcga gcagggtcag accggttcca
     1681 gccgtgccca gcaccagggc cccggccagg atccgggtat ccggctccgg gaaggcggtg
     1741 agcatcgcca ccacgaccag gatcaccatc caggcccacc ccagcagccg catcaggacg
     1801 actacccggg tgagagtgcg atcgacgggc agcagtcggg aatcgtggtg actggcttcg
     1861 gtcatcgaga aaggctatcg ggcgcacctt cgccatccac ggtggtcaga gagaagccat
     1921 ggcgcgctcc aacgccgg
//
LOCUS       DATITW010000183         3017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_730083, whole genome shotgun sequence.
ACCESSION   DATITW010000183 DATITW010000000
VERSION     DATITW010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730083"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..2026
                     /locus_tag="VLS86_02520"
     CDS             89..2026
                     /locus_tag="VLS86_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSO49397.1"
                     /translation="MEQFEIVWRPSPARAAATHTARFIAAHRLGDFEALRERSIADPE
                     WFWESVVGYLGLPFDKPWRTVLDTSRGIPWATWFSGSLFNISRSCVDRWADESPDRLA
                     VRSQKESGETRELSFGALRDEVALLAGALRDLGVGRGDAVAVFLPMSAEAVVSLLAVA
                     RIGAVFVPVFSGYGADAVASRLRDPKPKVLICANGFLRRGSVVEMKEVADRAVELAGG
                     IEHLVVVAYTDLAGNSMQEGRDLWWGEALAGAEPHAVLPTAAEDPMLIAYTSGTTGRP
                     KAAVHVHGGFGVQVAAEGAFQFDFKRDDVVMWATDMGWIMGPWIVVAGLSNGAAVATY
                     DAAPDYPGPDRIWQVASDLELTFLGISPTLIRALQRHGSEQAGRHDLSKLRTFGSTGE
                     PWNPDPWWWLFRDIGGETRPIVNISGGTEIGACLLSVNLLQGIKPTSLGGPALGIDAD
                     VYDPEGKPIRGEVGELVVRGSWPGRTRGFWNEPDRYLETYWERFEDTWVHGDWASIDD
                     DGFWYLHGRSDDTLNVAGKRIGPAEIESVAVAFPEVVMAAAIGIPDEIKGEAIGLYVV
                     TADGVEPDDRLAAAIEAAVVETLGKSFRPKTILWVTDLPRTRSAKIMRRVVKALALGQ
                     EPGDLSSLENPESLEGLGPLP"
     gene            complement(2040..2636)
                     /locus_tag="VLS86_02525"
     CDS             complement(2040..2636)
                     /locus_tag="VLS86_02525"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HSO49398.1"
                     /translation="MSTQTRIGLVCFDGMEELDLVGPWEVLRMWQLSWPEDGAEVFTM
                     SLDGETVTCAKGMQIVTERPMAEAGRLDVIVYPGGRGTRGHLGDDRIGTWLRSLAAEG
                     TLVTSVCTGSLVLADAGLLRNRPATTYWSELGTLTRLDPTIEVRPDDRFVDSGEVITA
                     AGVSAGIDMALHLVARLSSPERAREVRRAIQYDPEPPV"
     gene            complement(2636..>3017)
                     /locus_tag="VLS86_02530"
     CDS             complement(2636..>3017)
                     /locus_tag="VLS86_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSO49399.1"
                     /translation="AGNEPGESGQRGVIVNVASIAAFEGQVGQAAYSASQGGVVGMTL
                     PLARDLAGLGIRVVTVAPGIMDTPLLDGAPDELREALAAIHVFPKRLGTPEDFAHLVQ
                     AIVENPLLNGEVIRLDAAARMPPK"
BASE COUNT          493 a         1002 c         1017 g          505 t
ORIGIN      
        1 ctctgatggc gaggccataa gcgggcagcg taggcggccg cgggtgtcga cgatggctcc
       61 tagcctcccc cccgggtaga ggagggtgat ggagcagttt gagatcgtct ggcggcccag
      121 cccggctcgg gccgcggcca cccacaccgc gcgcttcatc gcagcgcatc ggctggggga
      181 cttcgaagcc ctgcgagagc gctcgatcgc agaccctgag tggttctggg aatcggtggt
      241 cggctatctc ggcctcccat tcgacaagcc atggcgaacc gtgctcgaca cctcgcgggg
      301 gatcccgtgg gcgacatggt tctccggatc cctcttcaac atcagccggt cctgtgttga
      361 ccgctgggca gatgagtctc cggatcggct cgcagttcgc tcccagaagg aaagcggcga
      421 gacccgggag ttgagtttcg gcgctctccg ggacgaggtc gcgctgctgg ccggcgcact
      481 ccgggatctt ggagtggggc gaggcgatgc cgtcgcggtg ttcctgccga tgtcagcgga
      541 ggcagtggtg tccctgctcg cagtggcccg aatcggcgcg gtgttcgtcc cggtcttctc
      601 cggctacggg gcggacgccg tagccagccg tctccgggac ccaaagccaa aggtcctcat
      661 ctgtgccaat gggttcctcc gtcgaggaag cgtggtggag atgaaggagg tcgccgaccg
      721 ggctgtcgag cttgccggtg gcatcgagca cctcgtggtc gtggcctaca ccgacctggc
      781 tggcaactcg atgcaagaag gtcgcgacct gtggtggggg gaggccttgg ccggtgccga
      841 acctcatgca gtgctcccca ctgccgcgga ggacccgatg ctcatcgcct atacctcggg
      901 caccaccggc cgccccaaag ctgcggtcca cgtccatggc ggtttcggcg ttcaggttgc
      961 agccgagggg gctttccaat tcgatttcaa gcgtgacgac gtcgtgatgt gggccaccga
     1021 catgggctgg atcatgggcc catggatcgt ggtcgccggc ctgtccaacg gagccgcggt
     1081 cgcgacctat gacgcggcgc cggactaccc gggtccggat cggatctggc aggtcgcctc
     1141 cgatctggag ctgaccttcc tcggcatctc ccccaccctg atccgggcgc tgcagcgaca
     1201 cggcagcgaa caggcgggtc gtcacgatct gtccaagctg aggacgttcg gatcgaccgg
     1261 ggagccgtgg aaccccgacc cctggtggtg gctgttccgg gacatcggag gtgagacccg
     1321 ccccatcgtc aacatatcgg gcgggaccga gatcggggct tgtctgctct cggtcaacct
     1381 gcttcaagga atcaagccca cgtccctcgg cggccccgcc ctcggaatcg acgccgacgt
     1441 ctacgaccct gaggggaaac cgattcgcgg cgaggtaggt gaattggtgg tccgtggctc
     1501 ctggccgggg cgaaccagag gcttctggaa cgagccggat cgctatctcg agacctactg
     1561 ggagcggttc gaagacacct gggtgcatgg cgattgggcc tcgatcgacg acgacggctt
     1621 ctggtacctc cacggccgat ccgacgacac gttgaacgtc gccggcaagc ggatcgggcc
     1681 cgccgagatc gagtcggtcg cggttgcctt cccggaggtg gtgatggcgg ccgcgatcgg
     1741 catccccgac gagatcaaag gggaggcgat cgggctctac gtggtgactg cggacggggt
     1801 cgagcccgac gatcggctgg ccgcagcgat tgaagcggcc gtggtcgaga ccctggggaa
     1861 gagctttcgc cccaagacca tcctttgggt gaccgacctg ccccgtaccc gctccgccaa
     1921 gatcatgcgg cgcgtggtca aggccctggc attgggccaa gagccgggag acctgtcgag
     1981 tctggagaac ccggagagcc tggaggggct tggcccgctg ccttgaagtc ggccgccaat
     2041 caaacaggcg gctccgggtc gtattggatc gcccgtcgca cctctcgggc gcgttccggg
     2101 gacgacagtc gggccacgag atgtagcgcc atgtcgatcc cggccgacac tccggcggcg
     2161 gtgatcacct cgccggagtc cacgaaccgg tcatccggcc tgacctcgat ggtcggatcc
     2221 aaccgggtca gcgtccccaa ctccgaccaa tacgtggtgg ccgggcggtt gcgcagaagc
     2281 ccggcgtcgg cgaggaccag agaaccggtg cagaccgaag tcaccagggt gccttccgcg
     2341 gccaggctgc gcagccaggt ccctatccga tcgtccccca aatggcctct ggtgcctctc
     2401 ccgcccgggt acacgatgac atccaaccga ccggcctctg ccatcggcct ctcggtcacg
     2461 atctgcatcc ctttggcaca ggtcacggtc tccccgtcga gggacatggt gaagacctca
     2521 gcaccgtcct ccggccacga gagctgccac atccggagca cctcccaggg gccgaccaga
     2581 tccaactcct ccatcccgtc gaaacagacc agcccaatgc gagtctgggt gctgatcact
     2641 tcggcggcat ccgggccgcg gcgtcgagac ggatcacctc accgttgagc aacgggttct
     2701 cgacgatcgc ctggacgagg tgggcgaagt cttcgggcgt ccccaggcgc ttggggaaga
     2761 cgtggatcgc agccagcgcc tcccgcagct cgtcaggggc gccgtcgagg agaggtgtgt
     2821 ccatgattcc gggcgccacc gttaccaccc gaatcccgag accggccaga tcccgagcga
     2881 gagggagggt catccccacc accccgccct gggatgccga gtaggcggcc tggcccacct
     2941 ggccctcgaa tgcggcaatc gaggccacgt tgacgatcac ccctcgctgg cccgactccc
     3001 ctggttcgtt gccggcc
//
LOCUS       DATITW010000184         1502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_49205, whole genome shotgun sequence.
ACCESSION   DATITW010000184 DATITW010000000
VERSION     DATITW010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49205"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VLS86_02535"
     CDS             complement(<1..648)
                     /locus_tag="VLS86_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49400.1"
                     /translation="MRRSEIMRRGLVLAVTLGVALFSVVAPAAASHTSDHAADQIRAI
                     VAQAEQDMEQTVSGFEAQSAGFDNQGQVDAAEDDATQAIETIWTDAKTAIEDLVKLYP
                     GELGNEGGTAKQEIQDKRQASRTEIASIANSWAPAATTTTTAPPATTTTTTTTPATTT
                     TRPPNQGNGVGPPAAGTNNGGGNSGGNSGGNSGGNSGGNSGGSGGVTAPPEATPVE"
     gene            718..>1502
                     /locus_tag="VLS86_02540"
     CDS             718..>1502
                     /locus_tag="VLS86_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSO49401.1"
                     /translation="MRKANSDYLQILIVGATLAAVLVLAVFLVNRESSGAIAADAEVA
                     ILSESTMSAASGVLNASNFAVVLAVNDADPDLVADARIGVENAVEELGSRVDRLLSSL
                     PADEQTPVDQAHRRFLLAINGALRNLDVFDAEAGTEVLADHGRIYDHLIDTLAEIRDA
                     RAGDILIAGEGVGRAAGAVRFLVVFLLPLGIMLALWKATRRGAAARILTEELRHEREV
                     SKSKDAFLADVSHELRPPLTGIYGLALALEDDPALEPSARELVG"
BASE COUNT          219 a          472 c          496 g          315 t
ORIGIN      
        1 ttcgacggga gtggcttcgg gtggcgccgt gaccccaccg ctgcctccgc tgttgcctcc
       61 gctgttgcct ccgctgttgc cgccactgtt gccgccacta ttgcccccac cgttgttggt
      121 ccctgccgcc gggggcccga cgccattccc ctggttcggg ggccgggtag tggttgtggc
      181 cggagtggtc gtggtcgtgg tcgtggtcgc gggcggagcg gtggtagtgg tggtggcggc
      241 cggcgcccac gaattggcga tcgaggcgat ctcggtgcgt gatgcctgac gcttgtcctg
      301 gatctcctgt tttgcggtcc caccctcgtt gcccagctct cctggataga gcttgaccag
      361 gtcttcgatc gcggtcttgg catccgtcca aatcgtctcg attgcctgag tggcgtcatc
      421 ttcggcggcg tcgacctggc cctggttgtc gaagccggcg gactgagcct cgaatccgga
      481 gacggtctgc tccatgtctt gctctgcctg ggccacgatg gcgcggatct ggtcagctgc
      541 gtggtctgat gtgtgactgg cggcggcggg agcgacgacg ctgaacagag caacgccgag
      601 cgttacagcg agtaccagcc cccttctcat gatttcactt cttctcatcg tcagaacggt
      661 ggttttcctt gatgggtcgc ttaaagccgg agaggcgtac tgccgaagaa gaaacaggtg
      721 cgcaaggcca attccgacta tctccagatc ctcatcgtgg gcgcgactct cgccgcggtg
      781 ctcgtgctcg ccgtgttcct cgtcaaccgc gagagctcgg gggcgatcgc ggccgacgcc
      841 gaggtggcga ttctctccga atcgacgatg agtgccgcct cgggggtgct caacgccagc
      901 aatttcgcgg tggttctggc ggtcaacgac gccgaccccg atctcgtcgc cgatgcccgc
      961 atcggtgtcg agaatgcggt cgaggagctc gggtcgcgcg tcgatcgtct cttgtcctca
     1021 cttcctgctg acgagcagac tcccgtcgac caggcacacc gcagatttct gttggcaatc
     1081 aacggtgctc tgagaaatct cgatgtcttc gacgccgagg ccggaaccga ggtgttggcc
     1141 gatcatggcc gaatctacga ccacctgatc gacactctgg ccgagatccg cgatgcgagg
     1201 gcgggcgaca tcctcatcgc cggggaaggg gtgggcaggg cggcaggtgc tgtccgcttc
     1261 ctggtggtgt tcctgctccc actcggaatc atgctcgctc tgtggaaggc aacccgacgt
     1321 ggggcggcgg cgcgcatcct caccgaggag cttcgtcacg agcgcgaggt gtccaagtcg
     1381 aaagacgcct tcctcgccga tgtctcccac gagttgagac cgccactcac cgggatctat
     1441 ggcttggccc tggcgctgga agacgacccc gcgctcgaac catcggcccg agagctggta
     1501 gg
//
LOCUS       DATITW010000185         2950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_690093, whole genome shotgun sequence.
ACCESSION   DATITW010000185 DATITW010000000
VERSION     DATITW010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690093"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VLS86_02545"
     CDS             complement(<1..895)
                     /locus_tag="VLS86_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HSO49402.1"
                     /translation="MERPAFTIGIEEEYLVVDRESRDLIKSPPPEMWDSLREVLGPQV
                     TPEFLKAQIEVGTKVCTRVQDAREDLANLRRDLSSVVNEYGAAIIAASTHPFANWAEQ
                     ETTEHPRYTNLATDYQQVARQLVICGMHIHVGIEDPHLRIDLMNQVKYMLPHLLALTT
                     SSPFWAGSPTGLLAYRLIIFRNLPRTGLPQDFLSWGEYERTVDLMIDAGIIDSPSSLW
                     WDIRPSGRYPTLEMRVSDVCTRLDDAITVAAIYVCLLSMLFRLRRQNQRWRTYPPFLI
                     AENLWRAQRYGTDGSLVDFGKG"
     gene            complement(908..1513)
                     /locus_tag="VLS86_02550"
     CDS             complement(908..1513)
                     /locus_tag="VLS86_02550"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HSO49403.1"
                     /translation="MELVIGTDGRPRCSWGSEPEIYRVYHDDEWGRAVTEDRRLFEKI
                     CLEGFQAGLSWLTILRKRDSFRAGFAGFDPESISLFDQDDVDRLLNDSGIVRHEGKIR
                     STINNAGRALEMIAEHGSLASYFWQWADPGDSPPNLEVAFTEASAAISKDLKKRGWSF
                     VGPTTIYAFMEAMGLVNDHLVGCHAREECARERQHVIELDL"
     gene            1588..>2950
                     /locus_tag="VLS86_02555"
     CDS             1588..>2950
                     /locus_tag="VLS86_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSO49404.1"
                     /translation="MGSTRARDSLIGAAAAALALGVSELAAGLLGLPSLIEGLGNWVI
                     DSVPTAIKDWAIATFGTNDKLVLLIGIALVTVAVGAVVGVLARERFGIAIAVFVGFAV
                     VAALAASRDPRVSLGTAIIPAGLAAISGLGALQWLYGLTGGTAGEETGESREQDGSRR
                     RVILGIGAVVGLAAVSAGAGRYLLDRAKRTVAGREEVVLPVPAQALAPVSPASDLGIP
                     GLSPVITPNATFYRIDTAFSVPRVDIQEWSLTVEGRVDRPYSISYFDLLDMRMVERDV
                     TLSCVSNQVGGDLVGNARWLGVPLVEILDRAGVQEGAEQLVGRSVDDFTVGFPVEAAY
                     DGREALVAVAMNGEPLPIEHGFPARLVVAGLYGYVSATKWLSSIELTGWDEFDAYWIP
                     RGWSKEAPIKTQSRIDTPQSNGQIEPGPRQVAGVAWAPHRGITKVEVQLGEDADWVEA
                     VLSE"
BASE COUNT          478 a          913 c         1008 g          551 t
ORIGIN      
        1 cgcccttgcc gaaatcgacg agcgagccat cggtcccgta acgctgggcg cgccacaggt
       61 tctcggcaat caagaacggt gggtaggtgc gccatctctg gttctgtctt cggagacgga
      121 acaacatcga cagcaggcac acgtagatcg ccgccaccgt gatcgcgtca tcgagacggg
      181 tgcagacgtc actcaccctc atctccaggg tggggtagcg ccccgagggt cgaatatccc
      241 accacaggct gctcgggctg tcgatgatcc cggcgtcgat catcaagtcg accgtgcgct
      301 cgtactcacc ccacgagagg aagtcctgag ggagaccggt tcgggggaga ttgcggaaga
      361 tgatcagccg gtaggcgagg aggccggtcg ggctcccggc ccagaacggc gaggacgtgg
      421 tcagcgccag aagatggggg agcatgtact tgacctggtt catcaggtcg atccggagat
      481 ggggatcttc gatcccgacg tggatgtgca tcccacagat caccagctgg cgtgccacct
      541 gttggtagtc ggttgccagg ttggtgtacc ggggatgctc ggtggtctcc tgttccgccc
      601 agttggcgaa tgggtgggtg gaggcggcga tgattgctgc cccgtactcg ttgaccacgc
      661 tggagaggtc ccgtctcagg ttggcgagat cctcgcgggc gtcctggact cgggtgcaca
      721 ccttggtccc gacttcgatc tgggccttga ggaattccgg tgtcacctgg gggccgagca
      781 cctcgcgcaa cgagtcccac atctcggggg gcgggctctt gatgaggtcg cgtgactctc
      841 ggtcaacgac gaggtactcc tcctcgatgc cgatggtgaa tgccgggcgc tcgatcatga
      901 gcgcacccta gagatcgagt tcgatcacat gctgtcgctc tcgggcgcat tcctctcggg
      961 cgtggcagcc cacgagatgg tcgttgacca ggcccatcgc ctccatgaag gcgtagatcg
     1021 tcgtcggccc gacaaacgac catcctcgct tcttcaagtc cttggatatc gcggcggagg
     1081 cttcggtgaa cgccacctcg agattggggg gcgagtcgcc cggatccgcc cattgccaga
     1141 agtaggaggc gagggaaccg tgctctgcga tcatctccag ggctcgaccg gcattgttga
     1201 tcgtggagcg gatcttcccc tcgtgacgga cgatccccga gtcgttcaac aggcgatcga
     1261 cgtcgtcctg atcgaagagg ctgatcgact cggggtcgaa tccggcgaat ccggcgcgga
     1321 agctgtcccg cttgcgaagg atggtcaacc aggagagacc ggcctgaaaa ccctcgaggc
     1381 agatcttctc gaacagacgg cggtcctcgg tgaccgcccg gccccactca tcgtcgtgat
     1441 agaccctgta gatctcgggc tccgagcccc atgagcagcg gggccgaccg tcggtgccga
     1501 tgacgagctc catatccgga cactttttac tcgtttgttt ctatttgctt tagcaccggt
     1561 tatccgatgt ccatctaggt tgttgccatg ggctcgacac gagcacgaga ttccctcata
     1621 ggcgccgccg cggctgcgct cgctctcggc gtgtcggagc tggccgccgg gctgttgggt
     1681 ctcccctcgc tgatcgaggg gttggggaac tgggtgatcg acagcgtgcc gaccgcgatc
     1741 aaggactggg cgatcgcgac cttcggcacc aatgacaagc tggtgctgct gattggcatc
     1801 gccctggtca cggtggcggt cggggcggtc gtcggcgtgc tggcccggga gaggttcggg
     1861 atagccatcg cggtcttcgt cggcttcgcg gtggtcgccg ccctggccgc ctcccgtgac
     1921 ccgagggtct ccctggggac cgcgatcatt ccggccggac tggccgcgat ctccggtctg
     1981 ggcgccctcc agtggttgta cgggctgact ggcgggaccg ccggggaaga gaccggcgaa
     2041 tccagggagc aggacggctc acgacgaagg gtcattctcg gcatcggggc ggtggtaggt
     2101 ctcgccgcgg tgtcggccgg cgccggccgt tatctcctcg acagggcgaa gcggacggtt
     2161 gcggggcggg aggaggtggt cctgcctgtg ccggcccagg cgctggcccc ggtcagtccc
     2221 gcgtcggacc tcgggatccc cggtctcagc ccggtcatca caccgaacgc caccttctac
     2281 cgaatcgaca ccgccttctc cgtgccacgg gtcgacatcc aggaatggtc gctgacggtg
     2341 gaagggcgtg tcgatcgtcc ctactccatc tcgtacttcg atcttctcga catgcgcatg
     2401 gtggaacgcg acgtcacgct ttcttgtgtg tccaatcagg ttgggggcga cctggtggga
     2461 aacgcacgat ggctgggggt gcctctcgtc gagatcctcg accgggccgg ggtccaggag
     2521 ggcgccgagc agctcgtggg acgctcggtc gacgacttca ctgtcggatt cccggtcgaa
     2581 gccgcctacg acgggcggga ggccctggtc gcggtggcga tgaacgggga gccgctgccg
     2641 atcgagcacg ggttcccagc ccgtctcgtc gtcgccggtc tctacgggta tgtgtccgcc
     2701 acgaagtggc tgtcgtcgat cgagctgacg ggatgggatg agttcgacgc ctactggatc
     2761 ccccgcggct ggtcgaagga agcaccgatc aagacccaga gccgcatcga cactccccag
     2821 tcgaacggcc agatcgagcc cggcccgcgc caggtggctg gagtcgcctg ggccccccac
     2881 cgcggcatca ccaaggtgga agtccagctc ggcgaggacg cggactgggt ggaagcggtg
     2941 ctctccgagc
//
LOCUS       DATITW010000186         3230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_49224, whole genome shotgun sequence.
ACCESSION   DATITW010000186 DATITW010000000
VERSION     DATITW010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49224"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /locus_tag="VLS86_02560"
     CDS             <1..1471
                     /locus_tag="VLS86_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HSO49405.1"
                     /translation="SVARRTQFRLLVALTIVWALWAGWSLVGRATPYTLRVLDDTGAP
                     IAAASVDIRNRQIGTSAGDGTIEVEWRRSNSVLEVSAAGHIPKTLTLTERPQGVVDVV
                     LNARILRGRVTDAAGAPVEAVQVVAGPAQGATDLEGRFSLRGAEPGPVSVSRPAWVET
                     SFEWDGGVGEAVVEMTPFVARAVHISGEAAGEDIDRFFEMAESTELNALMLDLKDESG
                     LVWYDSEDPVADEVGAIRGLYDLSEIASRADQADLYLIGRLVVFNDPTAAIRKPSMSV
                     WDADLDQPYSADGQYFLDPTDPEAQAYGLSLAEEVCEMGDDEVQFDYVRFPDARSDHA
                     TFDGGVGLEVRTSTIADFLRQAVALLHPMGCAVAADVFGFTTAPGTDDGGIGQRWEDV
                     TQIVDVASPMVYPSHYGEGWYDFETPNDHPGPMVTRAIEDGLGRLPRNVVVRPWLQDF
                     GYTPDQVRAQIESAEQFGLGWMLWNATSTVTVDALTRIE"
     gene            1527..2789
                     /gene="sufD"
                     /locus_tag="VLS86_02565"
     CDS             1527..2789
                     /gene="sufD"
                     /locus_tag="VLS86_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HSO49406.1"
                     /translation="MTAFDSEKLAQIKASAPEWRTGQMDAAYQVFTGLDEPTGAEEDW
                     RYVDFDLRFDELTPVGAPGAPLDPGPFVASLPERSGHVLIVDGSILEISSDIASVKRF
                     SELDTDPGVLGLVPVDLNKLAAAHAAFADDGVLVEVDRGVVMETPLVVDVQAATAGTA
                     SFPHLMVRVGENAEARVVVVYRSADGARLLMVPEVDLEVGDGGRLRFLSVQGLDHAAS
                     TVVHQRVVLGRDATSRIGEVGLGGKLARLDLGVSLVGNGSSSELVGLYFGEGDQTLDY
                     RVVIDHEGKNTTSDVFLKGAVEDDAQSVFTGLLRIEKDATRTSTFETNRNLVLSENAK
                     AQSVPNLEILCNDVICGHASSVGPLEREHLYYLQSRGLTRERAERLLIRGFFQEVIDR
                     LPIEGLEAPVSEEVFRRFVTAQEEGRVA"
     gene            2786..3097
                     /locus_tag="VLS86_02570"
     CDS             2786..3097
                     /locus_tag="VLS86_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HSO49407.1"
                     /translation="MRVEIGSIDALPRDRGVRVTIGEERIAMFRVGNDVFAIGDRCSH
                     AEASLAEGEVFDRVVECPRHGSEFDLATGNAMSLPATKPVPTYPVSVEDGTVYLLLGD
                     S"
     gene            3094..>3230
                     /locus_tag="VLS86_02575"
     CDS             3094..>3230
                     /locus_tag="VLS86_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO49408.1"
                     /translation="MTAALEVRELSAGVEGKQILDELDLEIPFGQVHAIMGPNGSGKS
                     TL"
BASE COUNT          538 a          985 c         1118 g          589 t
ORIGIN      
        1 ttcagtggcg cgacgaaccc agtttcgatt gctggttgcg ctcaccatcg tctgggcact
       61 ttgggccggc tggtcgctgg tcggtcgtgc gactccctac acgctgcggg ttctcgatga
      121 caccggggcg ccgatcgcgg ctgcctcggt cgacatcagg aaccgtcaga tcgggaccag
      181 cgccggagac gggacgatcg aggtcgaatg gcggcgatcc aactccgttc tcgaggtgag
      241 tgctgccggt catatcccca agacgctcac cctcaccgag cgcccgcaag gggtagtcga
      301 cgtggtgctc aatgcgcgca ttctgcgcgg gcgggtcacc gatgccgccg gcgccccggt
      361 ggaggcggtc caagtggtag cggggccggc ccagggcgcc accgatctcg agggacgatt
      421 cagcctcaga ggggcagagc ctggtccggt atcggtgtcc cggccggcat gggtcgagac
      481 cagtttcgaa tgggacggag gggtcggtga ggccgtcgtg gagatgacgc ccttcgtcgc
      541 ccgggcggtc cacatctcgg gtgaggccgc cggggaggac atcgatcgtt tcttcgagat
      601 ggccgaatcc accgagctca atgcgctgat gctcgatctc aaggacgaat ccgggttggt
      661 ctggtacgac agcgaagacc cggtggcaga cgaggtcggg gcgatacgcg gcctgtacga
      721 cctgagtgag atcgcgtcca gggccgatca ggccgatctc tatctgatcg gtcgcctggt
      781 ggtcttcaac gacccgaccg cggcgattcg gaagccttcc atgtcggttt gggacgcgga
      841 cctcgaccag ccgtactcgg cggacggcca gtacttcctc gacccgaccg atccggaagc
      901 gcaggcctat gggctctcgc tggccgagga ggtctgcgag atgggtgacg acgaggtgca
      961 gttcgactat gtcaggttcc ccgacgccag gtccgaccac gcgaccttcg atgggggtgt
     1021 cggtctcgag gttcggacca gcaccatcgc cgatttcctt cgtcaagcag tggcgttgct
     1081 ccaccccatg gggtgtgcgg tggcagcgga cgtgttcggc ttcaccacgg cgccgggcac
     1141 cgacgacggg ggcattggcc agcgatggga ggacgtgacc cagatcgtcg acgtcgcttc
     1201 gccgatggtc tacccgtcgc actacgggga gggttggtac gacttcgaga cacccaacga
     1261 tcatcccggg ccgatggtca cccgtgccat cgaagacggg ctgggcagac tccccagaaa
     1321 cgtcgtggtg cgcccctggc tgcaggactt tggctacacc cccgatcagg tccgggcgca
     1381 gatcgagtca gccgagcagt ttggtctggg ctggatgctg tggaacgcca catcgacggt
     1441 gactgtcgat gcgctgacga ggatcgagta attcgcacta tccgcgtagg tgaaatagca
     1501 tcagccggtc ccctccggag cactccttga ccgcgttcga ctctgaaaaa ctggctcaga
     1561 tcaaggcttc cgcccccgag tggcgaaccg ggcagatgga tgccgcctat caggtgttca
     1621 ccgggctgga tgagccaacc ggggccgagg aggactggcg gtatgtcgat ttcgatcttc
     1681 ggttcgacga gctcaccccg gtcggagcac cgggcgctcc gctcgacccg ggcccatttg
     1741 tggcttcgct cccggaacga tccgggcatg tgctgatcgt cgacgggtcg atcctcgaaa
     1801 tctcctctga catcgcctcg gtcaagaggt tctccgaatt ggacaccgac cccggtgtcc
     1861 tcggcctggt tccggtggac ctcaacaagc tggccgcggc tcacgccgcc ttcgccgacg
     1921 atggggtgct cgtcgaagtg gatcgtggcg tagtcatgga gaccccgctg gtggtcgacg
     1981 tacaagcagc gactgcaggc acggcctcgt tcccccatct gatggtgagg gtgggtgaga
     2041 acgcggaggc cagggtggtc gtggtctacc ggtcggccga cggggcgagg ctcctcatgg
     2101 tccctgaggt cgacctcgag gtcggagacg ggggaagact ccggtttctc tcggtgcagg
     2161 gtctcgacca tgcggcgtcc accgtggtcc atcaacgggt ggttctgggt cgtgatgcga
     2221 cctcgcggat tggagaggtg gggctcggtg gcaagctggc tcgtctcgac ctcggggtga
     2281 gcctggtcgg gaacgggtcg tcctcggagt tggtcggcct ctacttcggc gagggggatc
     2341 agaccctgga ctaccgggtg gtcatcgacc acgagggcaa gaacaccaca tcggacgtct
     2401 tcctcaaggg cgcagtcgag gatgacgcac agagtgtgtt caccgggttg ctccggatcg
     2461 agaaggacgc gacccgaacc tccaccttcg agaccaaccg caatctggtc ctatcggaga
     2521 acgccaaggc gcaatccgtg ccgaatctgg agatcctgtg caacgacgtg atatgtggcc
     2581 atgcctcttc ggtcggccct ctggaacgtg agcatctcta ctacctgcag agccgcggcc
     2641 tcaccaggga gcgcgcggag cggcttctga taagagggtt cttccaggag gtcatcgatc
     2701 gtcttcccat cgaagggttg gaggccccgg tctcggaaga ggtcttccgg aggttcgtca
     2761 ccgcccagga ggagggccgg gtcgcgtgag ggtcgagatc gggtccatcg acgccttgcc
     2821 ccgcgatcgc ggggtgcggg tcacgatcgg cgaggagcgc atcgccatgt tccgggttgg
     2881 caacgacgtc ttcgccatcg gcgaccggtg ctcccacgcc gaggcgtcac tggccgaggg
     2941 tgaggtgttc gaccgggtgg tggagtgccc ccgacacgga tccgagttcg acctggcgac
     3001 cggtaacgcc atgtcgctgc ccgccaccaa gccggtcccg acctacccgg tgtccgtcga
     3061 ggatggaacc gtctatctgc tgctggggga ctcatgacgg cggcgctgga ggttcgcgag
     3121 ctgtcggccg gtgtggaagg caagcagatc ctcgacgagc tcgatctcga gatcccgttc
     3181 gggcaggtgc acgcaatcat gggccccaac ggctcgggga agtcgacgct
//
LOCUS       DATITW010000187         3625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_329643, whole genome shotgun sequence.
ACCESSION   DATITW010000187 DATITW010000000
VERSION     DATITW010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329643"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VLS86_02580"
     CDS             complement(<1..293)
                     /locus_tag="VLS86_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HSO49409.1"
                     /translation="MGKVVMYSSVSVDGFIADENDQPGPLFDWLSSGDVPLDESGVLK
                     VSQTSYDYIRPYWDQIGATLVGRHVFDMTDGWDGKPPSGIDHVVVVTHRPKPEG"
     gene            complement(360..518)
                     /locus_tag="VLS86_02585"
                     /pseudo
     CDS             complement(360..518)
                     /locus_tag="VLS86_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(647..723)
                     /locus_tag="VLS86_02590"
     tRNA            complement(647..723)
                     /locus_tag="VLS86_02590"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(687..689),aa:Pro,seq:cgg)
     gene            848..1150
                     /locus_tag="VLS86_02595"
     CDS             848..1150
                     /locus_tag="VLS86_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49410.1"
                     /translation="MTAYDAMLRLKGTDDSPIHVLIDLTEDRFTMTAAEIEVASWARD
                     EIRVAPQVDGFHIRAEGEEIILDVTEDARFALDLGLRNAPPLLRRRMSALLRASED"
     gene            complement(1243..2259)
                     /locus_tag="VLS86_02600"
     CDS             complement(1243..2259)
                     /locus_tag="VLS86_02600"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HSO49411.1"
                     /translation="MSDRAVAIVGATGAVGLTMRAVLEESDFPLGSLRLMASERSAGT
                     SIDTSWGEVEVEDLATADPTGIDIALFSAGGDRSRAHAPRFAEAGAVVIDNSSAFRMD
                     PRVPLVVAGVNDAEANTHEGIIANPNCNTMTLMMAAGPLHRAAGLRSMTATSYQSVSG
                     SGQQGVVELISQTDRLRPDLDALMTGGWVDPVPSLYARPIGFNVLPYAGVETEQGYTD
                     EEWKLVNESRKILDIPDLLVEPTCVRVPVLVGHGVAATLRFERPVAADEARGILAVAP
                     GVPVWEDRVPTPLDSAGIDDVLVGRIRPTIGEPGGINLWVVGDNLRKGAALNAVQIAE
                     ILVR"
     gene            complement(2256..3488)
                     /locus_tag="VLS86_02605"
     CDS             complement(2256..3488)
                     /locus_tag="VLS86_02605"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HSO49412.1"
                     /translation="MSIVVQKFGGTSLADSARIKAVADRVEATRRDGNEVVVVVSAMG
                     ETTDQLVSLAAELSDDPSPREMDMLLSAGERISMALLAITLNSRGIPAVSFTGSQAGI
                     LTDSSHGSAKILDIKGTRVRDSLAQGRVVIVAGFQGVDPESKEVTTLGRGGSDATAVA
                     LAAALGADSVEIYTDVDGVFTADPRIVPEASKLDEVPFDEMLELSAGGAGVLMSRAVE
                     FGRRFEIPIHVRSSFHDGEGTWVRESSMEQAIVSGIAHDRSEAKLTVNRVPDRPGVAA
                     ALFGSLARAGVNVDMIVQNVSHDGTTDISFTVPRVNVVEGERIAKQVAVEIGAESVDV
                     DSEIAKVSLVGAGMKTHPGVAARVFEAMSEAGINIEMISTSTIRISCVIRAGQVEAAV
                     QALHDAFEPPTIFEEVGA"
BASE COUNT          634 a         1206 c         1185 g          600 t
ORIGIN      
        1 ccctccggct tcggccggtg cgtcacgacg accacgtggt cgatcccgct cggaggcttc
       61 ccgtcccagc cgtccgtcat gtcgaagacg tggcggccga cgagtgtcgc cccgatctgg
      121 tcccagtacg gccggatgta gtcgtaggac gtctgcgaca ccttcagcac gccgctctcg
      181 tccagcggga cgtcaccgct ggacaaccag tcgaacaggg gtccgggctg gtcgttctcg
      241 tccgcgatga agccgtccac cgacaccgag ctgtacatga ccaccttgcc catcgggctt
      301 ctcctttgct ttgggatgcc cccaaattaa ttgtcgtgag ctgtcgctct tgtaagaatt
      361 caatcggccg gcagcggcca gccgtccagc gcgtggccgg gatgttcgcg caggaaccgc
      421 caccggattt cgacgtaccg ggtcggcgtg agcccggtga acgctcggaa ctcgtggccg
      481 aaatgggcct ggtcgaaata gcctgcgcca ccggcgaggg tcggagcctg atctatgaaa
      541 ggcagggtgt ccatcgcagg agaccacagg ccgcgcggga acgcgggatg gatcaaggaa
      601 ggccctggtc agggccccgg ttccagagcc ctgaccagga cctttgtggt cgggacggtg
      661 ggacttgaac ccacgacctc agcgtcccga acgctgcgcg ctaccaagct gcgctacgtc
      721 ccgatcggct tgatgctagg cgctcccggg ggtctagccc atttggatca acggagaatg
      781 cactgttgcc cccaccgatc ggctgtagcc tttcccgtgg agggctgcta cctgcactgg
      841 gaggaaaatg accgcgtacg acgccatgct tcggctgaag gggaccgatg acagtcccat
      901 ccacgtgctg atcgacctca ccgaagaccg gttcaccatg accgcggcgg agatcgaggt
      961 ggcgagctgg gcgcgggacg agattcgggt ggcaccccag gtcgacggct tccacattcg
     1021 cgccgaaggc gaagagatca tcctcgacgt caccgaggat gcgcggttcg cgctcgacct
     1081 cgggctgcgc aacgccccgc cattgctccg acgccggatg tcggcgctgc tccgcgcctc
     1141 ggaggactag ggacatcgtc tccccaccca ccgtaattgg gacggcaggg ggtcatatcc
     1201 gaacctgagc cacccaagaa cgggtaagcg ggccaggaag ggtcagcgga ccaggatctc
     1261 ggcgatctgc accgcgttca gcgccgcccc tttgcgcaga ttgtccccga caacccacag
     1321 gttgatccca cccggctctc cgatggtggg gcggatcctc cccaccaaga catcgtcgat
     1381 tccggccgag tccagaggag tggggacccg gtcctcccac accggcactc cgggggcgac
     1441 agccagtatc ccccgggcct catcggcagc gaccggtcgc tcgaagcgaa gcgtggcggc
     1501 gacgccgtga ccgaccagca ccgggacccg aacacaggtg ggctcgacca ggaggtcggg
     1561 gatgtcgagg atctttctcg actcgttgac cagcttccat tcctcgtcgg tgtacccctg
     1621 ctcggtctcc acgccggcat aggggagcac gttgaacccg atcggcctcg catagagcga
     1681 aggcaccgga tcgacccagc cgccggtcat cagcgcgtcg aggtcgggcc gcagccggtc
     1741 ggtctgcgat atcagctcga ccacaccttg ctggccggat ccggacaccg actggtagga
     1801 ggtagcggtc atggagcgga gaccggcggc ccgatgcagg gggccggcag ccatcatcag
     1861 ggtcatcgtg ttgcaattgg ggttggcgat gatcccctcg tgggtattcg cctcggcgtc
     1921 gttgaccccg gccacgacca gcggcacccg cgggtccatc ctgaacgccg acgagttgtc
     1981 gatgaccacc gcgcccgcct cggcgaagcg cggtgcatga gcgcgggagc ggtcgccgcc
     2041 ggctgagaag agtgcgatgt cgatgccggt cgggtcggcg gtggcaaggt cctcgacctc
     2101 gacctcgccc cagctggtgt cgatcgaggt gccggcggag cgctccgacg ccatcagacg
     2161 gagcgagccc agcgggaagt cgctctcttc gaggacggcc cgcatggtca gcccgaccgc
     2221 cccggtggcg ccgacgatgg ccacggcacg gtcgctcatg ctccgacctc ctcgaagatc
     2281 gtcggcggct cgaaggcgtc gtggagggcc tggacggccg cttccacctg acccgccctg
     2341 atcacgcacg agatgcggat ggtcgaagtc gagatcatct cgatgtttat acctgcttcg
     2401 gacattgctt cgaaaaccct ggcagcaact ccgggatgcg tcttcatccc ggccccgacc
     2461 agtgacacct tggcgatctc cgagtctacg tccaccgatt cggcgccgat ctcgacggcg
     2521 acctgcttgg cgatgcgctc cccttccacg acgttgaccc ggggaacggt gaagctgatg
     2581 tcggtggtcc cgtcatggga tacgttctgg acgatcatgt ccacgttgac cccggctcgg
     2641 gccagggaac cgaacagggc cgcagccacc ccgggacggt cggggacacg gttcacggtg
     2701 agcttggcct ctgatcgatc gtgggcgatg ccgctgacga tggcttgctc catcgacgac
     2761 tcgcgcaccc aggtgccctc accgtcgtgg aacgaggatc gaacgtggat cgggatctcg
     2821 aagcgacggc cgaactcaac ggcgcgggac atgaggacgc cggcgccgcc cgcggacagc
     2881 tccagcatct cgtcaaaggg gacctcgtcg agcttgctcg cctcgggaac gatgcggggg
     2941 tcggcggtga acaccccgtc gacatcggtg tagatctcca ccgagtcggc tcccagggct
     3001 gccgccagtg cgaccgcggt ggcatcggag cctccgcggc cgagagtggt cacctctttc
     3061 gattcggggt ccacgccctg gaacccggcg acgatcacca cccggccctg ggccaggctg
     3121 tcacggaccc tggtcccctt gatgtcgagg atcttggccg aaccgtggga cgagtcggtg
     3181 aggatccctg cctgggatcc ggtgaagctc accgcgggga ttcccctcga gttcaaggtg
     3241 atcgcgagca gcgccatcga gatcctctcc ccggcgctga gcagcatgtc catctcacgg
     3301 ggtgaggggt cgtcgctcaa ctcggcggcg agcgacacca gttggtcggt ggtctctccc
     3361 atggcggaaa cgaccacgac cacctcgttt ccgtcgcgcc gggtcgcttc gacgcgatcg
     3421 gccacagctt tgatccgggc cgagtccgcc aacgacgtgc caccgaattt ctggaccacg
     3481 atcgacatga tgagcacagt ttggatcgtt ttggcgcaga acagaaacac cactgagagg
     3541 ttcgagggca ccggtgccta aagtcgctgc gccatgaccg accgacgaag gcgccagaag
     3601 gaacagcgca ccgccaagag acagg
//
LOCUS       DATITW010000188         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_650064, whole genome shotgun sequence.
ACCESSION   DATITW010000188 DATITW010000000
VERSION     DATITW010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650064"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VLS86_02610"
     CDS             complement(<1..338)
                     /locus_tag="VLS86_02610"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HSO49413.1"
                     /translation="MRDILSDLVAEQQFLDQSLQRIPIKIWDMVTPSKPWTVRDTIAH
                     LADFEELAADTIAGGDKLRRWQTEPNLDALRNEAIKKGRGMRPQDDIEWWRGGRAKVV
                     EPLSHMDAGDR"
     gene            complement(389..1471)
                     /locus_tag="VLS86_02615"
     CDS             complement(389..1471)
                     /locus_tag="VLS86_02615"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HSO49414.1"
                     /translation="MPYGLGNSDLDRMVAELVAAATSIPDGDLIGEMITTALKLGRDG
                     ASRGDLKLINTALKEMRYSNLVFSRHTEPKVTIYGSARLHEGDPNYQITMEFASTMAR
                     SGWGVITGAGPGIMEAGNRCAGIDHSYGVNIRLPFESEANPYIDPNRVVNFKYFFTRK
                     LGFVKEAHAFVLLPGGWGTLDEAFELLTLMQTGKSDLHPIVLLEAEGTNYWGPMIEFI
                     KDRQLDKGLITDSDLTIFLHTVDQRAAAEHIWHFYANYHSQRYAGGRLILRMREGPDD
                     DELANLNQEFSDIIVEGGLERVESSPAEVGDVDALDLDRIAFVFDRRHFGRLRMLIDR
                     LNDLVVAPKDFHLPEPMTEDHAERPW"
     gene            complement(1534..>2337)
                     /locus_tag="VLS86_02620"
     CDS             complement(1534..>2337)
                     /locus_tag="VLS86_02620"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSO49415.1"
                     /translation="EGLVPLHIAVRGHMGTGKDHDIEQFAAAMGLPYFRIPLTGEVRD
                     VTLIGSTRLHGDGKGGTESRWEDGDITRALRGPSLLNLSELNAAGAETLFALHGLLDR
                     YGALDLPTGETVKLRPDVRIFGTMNPTDLRDYAGTQTLNKAFADRWVIWEKNFPGNDQ
                     VVEMLTRRYPSLNEQYVTAIARLAVEVNASFEAQDSGVRVETPLSLRSVLDRIPTGLV
                     VYSDRTDPLRHAWEEYVLPHVDQFDRDHYETVWNAVVRKGPTVKPFAAG"
BASE COUNT          389 a          748 c          758 g          442 t
ORIGIN      
        1 cggtccccgg catccatgtg ggacaggggc tcgaccacct tggccctgcc cccacgccac
       61 cattcgatgt cgtcttgcgg ccgcatcccg cgacccttct tgatcgcctc gttgcgcaag
      121 gcgtcgaggt tgggctcggt ctgccagcgc cggagcttgt cacccccggc aatggtgtcg
      181 gcggccaact cttcgaagtc ggcgagatgg gcgatggtgt cccggaccgt ccacggtttc
      241 gatggcgtca ccatgtccca gatcttgatc gggatcctct gtagggactg atcgaggaac
      301 tgctgctcgg caacgaggtc ggagaggatg tcgcgcacac cccgatgtta gtccccggcc
      361 attgcttagt cccaggggaa aagctcgctt accagggtct ttccgcgtgg tcctcggtca
      421 tcggctccgg cagatggaaa tccttgggag ccacgaccag gtcgttgagg cggtcgatca
      481 acatcctcaa ccggccgaaa tggcgccgat cgaagacaaa agcgatgcgg tccaggtcga
      541 gggcgtcgac atcgccgacc tcggccggac tcgactcaac ccgctccaga ccaccctcga
      601 cgatgatgtc ggagaactcc tggttcagat tggccagctc atcgtcatcg gggccttccc
      661 gcatcctgag gatcaacctc ccgcccgcgt atcgctgcga gtggtagttg gcatagaaat
      721 gccagatgtg ctcggcggcg gccctctgat cgaccgtgtg gaggaagatc gtgaggtcgg
      781 aatcggtgat caagcccttg tccagttgcc ggtccttgat gaactcgatc atcggccccc
      841 agtagttggt gccctcggcc tcgagaagga cgatcgggtg gaggtcgctc tttccggtct
      901 gcatcagagt caacagctcg aacgcctcgt cgagggtgcc ccatccgccg gggaggagga
      961 cgaaggcgtg ggcctctttg acgaatccga gctttcttgt gaagaagtac ttgaagttca
     1021 ccacccggtt ggggtcgatg taggggttgg cctccgactc gaaggggagc cggatgttca
     1081 ccccgtagga gtgatcaatg ccggcgcagc ggttgccggc ctccatgatc cccgggcctg
     1141 caccggtgat cacaccccat ccgctgcggg ccatggtcga ggcgaactcc atggtgatct
     1201 ggtagttcgg gtcgccctcg tggagccggg ccgaaccgta gatggtcacc ttgggctcgg
     1261 tatgccggga gaagacgagg ttggagtacc tcatctcctt gagcgcggtg ttgatcagct
     1321 tcaggtcccc ccgtgacgcc ccgtcccgcc ccagcttgag ggcggtggtg atcatctccc
     1381 cgatcaggtc gccatccggg atcgaggtgg cggcggcgac cagctcggcc accatccggt
     1441 ccaaatccga gttgccgagg ccgtatggca tggttcgacg ctactcaact ccgggcgcgg
     1501 accgtgaccg gcggcggtct cgagcccctg gctctaaccg gccgcgaatg gcttcacggt
     1561 gggacccttg cgcaccactg cgttccaaac cgtctcgtag tggtcacggt cgaactggtc
     1621 gacgtggggc agcacgtatt cctcccaggc gtgtcgcagc ggatcggtgc ggtcggagta
     1681 gaccaccagc ccggtcggga tgcggtcgag caccgagcgg agcgagagcg gcgtctcgac
     1741 ccggacacct gagtcctggg cttcgaagga ggcattgacc tccaccgcca gccgggcgat
     1801 cgcggtcaca tactgctcgt tgaggctcgg atagcgtctg gtgagcatct cgacgacctg
     1861 gtcgttcccg gggaagttct tctcccaaat cacccagcgg tcggcgaacg ccttgttcaa
     1921 ggtctgggtg cccgcatagt cccgcaggtc ggtggggttc atggtgccga agatgcggac
     1981 atcggggcgc agcttgaccg tctccccggt cgggagatcg agggccccgt accgatcgag
     2041 cagaccgtgg agggcgaaca gcgtctccgc tcccgccgcg ttcaactcgg agaggttgag
     2101 cagcgacggg ccacgcagcg cccgtgtgat gtccccgtcc tcccaacggc tctcggtccc
     2161 gcccttcccg tctccgtgga gacgcgttga cccgatcagg gtcacgtcgc gcacctcacc
     2221 ggtcagcggg attcggaagt aggggagacc catggcagca gcgaactgct cgatgtcgtg
     2281 gtccttcccg gtccccatgt gaccgcgcac cgcgatgtga agcggcacca gtccctc
//
LOCUS       DATITW010000189         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_570231, whole genome shotgun sequence.
ACCESSION   DATITW010000189 DATITW010000000
VERSION     DATITW010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570231"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VLS86_02625"
     CDS             complement(<1..549)
                     /locus_tag="VLS86_02625"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HSO49416.1"
                     /translation="MVGAHVPSISPLDEAMERKADLVQIFLSDPQSWKKPAPRPDAED
                     LRRSPLPIYVHAPYLINVCADNNRVRVPSRRILQETCDAAAEVGAEAVVVHGGHVTGD
                     GPQEEGIDRWRKALDPLDSQVPVLIENTAGGDKAVARRLEWIKRLFDEIGGYGVGFVF
                     DTCHIWAGGEPLDEVVPRVMEAI"
     gene            complement(552..1139)
                     /locus_tag="VLS86_02630"
     CDS             complement(552..1139)
                     /locus_tag="VLS86_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49417.1"
                     /translation="MRVVVQRSAVKMWLLAIGSIPLLVISVDVLTNRRITNWLREMVF
                     NPNDTQIYEPRDVIWAWVMLLFSALLILWGLKELFVPTKVIEARAEGLAVRLRGPLAK
                     ADLIPWENVIDIRPGEIMDEGDVLILLMISLLTRGDLPDHPWGARWVETRVLGMLAQD
                     WATPPAKVAEQIAEFAAGVARHEAKARTARIWNPS"
     gene            complement(1136..1681)
                     /gene="pyrE"
                     /locus_tag="VLS86_02635"
     CDS             complement(1136..1681)
                     /gene="pyrE"
                     /locus_tag="VLS86_02635"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HSO49418.1"
                     /translation="MPSSALIDHLRTHALFTDGPYTLRSGARSDWYLDARRTTMSGDG
                     AHIVGRAVLEVLEPEVVAIGGMTMGADPIALAVAMVAHHGGRKLSAFSVRKTTKIYGI
                     GGRLVGPVSAGMAVAIVEDTTTTGGAALEAAEAAAAGGLQVVQAIALVDRSGGRAQEA
                     FDEHSIPYTALLLPKDLGVKT"
     gene            1744..>2350
                     /locus_tag="VLS86_02640"
     CDS             1744..>2350
                     /locus_tag="VLS86_02640"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSO49419.1"
                     /translation="MDLLLVRHGQPDWTPDRIARNDPDLTTLGHEQANRVAQRLGELE
                     RVDELWVSSMNRALQTSVPVSSEIEMVPEVHGWLQEINNPPSWDGSPADEIEKALRDS
                     NFRTMEEMWDGLPGGESFRAFHQRVVSGLEATLAALGITPMHGEHPHLWDVSDPNQRV
                     IVVAHAGTNAVVLGRLLGLEPVPWEWDRFRQPHTGVSRLTMS"
BASE COUNT          396 a          812 c          747 g          395 t
ORIGIN      
        1 gatggcctcc atgacccggg gcacgacctc gtcgagcggc tccccacccg cccagatgtg
       61 gcaggtgtcg aagacgaacc cgaccccgta ccctccgatc tcgtcgaaca gccgcttgat
      121 ccattccagt ctgcgggcca ccgccttgtc cccacccgcc gtgttctcga tcagcaccgg
      181 cacctggctg tcgagaggat cgagcgcctt gcgccatcga tcgatcccct cctcctgggg
      241 gccgtcgccg gtcacatgcc ccccgtggac cacgaccgcc tccgccccga cctcggccgc
      301 cgcgtcacaa gtctcctgga ggatgcgacg agacggcacc ctgacccgat tgttgtcggc
      361 acatacgttg atcaggtacg gggcatggac gtagatcgga agcggggatc ggcggaggtc
      421 ctcggcgtcg ggacggggag caggcttctt ccatgattgg ggatcggaga gaaagatctg
      481 caccaggtcg gcctttcgct ccatcgcctc gtccagggga ctgatcgagg gcacatgggc
      541 gccgaccagg gtcacgacgg gttccagatg cgagccgtcc tcgccttggc ctcgtgtctt
      601 gccacacccg cggcgaactc ggcgatctgc tccgccacct tcgccggggg agtcgcccag
      661 tcctgggcca gcatccccaa caccctcgtc tccacccatc gcgctcccca cgggtggtcg
      721 ggaaggtcgc cccgggtgag caggctgatc atgagcagga tgagcacgtc gccctcgtcc
      781 atgatctccc cgggccggat gtcgatcacg ttctcccagg ggatgaggtc tgccttggcc
      841 agaggcccgc ggagtctcac cgccagcccc tcggcccgag cctcgatcac cttggtcggc
      901 acgaacagct ccttcagccc ccaaaggatg agcaaggcgg agaacagcag catgacccag
      961 gcccagatga cgtctcgtgg ctcgtagatc tgagtgtcgt tcgggttgaa caccatctca
     1021 cgcagccagt tggtgatgcg acgattggtg aggacatcga ccgatatcac gagaaggggg
     1081 atgctcccga tcgccagcag ccacatcttg accgcggagc gctggaccac gaccctcatg
     1141 tcttgacgcc gaggtccttg ggcaacagca gagctgtgta ggggatggaa tgctcgtcga
     1201 acgcctcttg ggcgcgtccc ccggaacggt cgacgagagc tatggcctgg acgacctgga
     1261 gcccaccggc cgcagccgcc tccgcagcct cgagggctgc gcccccggtg gtcgtggtgt
     1321 cctcgactat cgccaccgcc atcccggcgg agacaggacc gaccagtcgg ccgcctatcc
     1381 cgtagatctt ggtggtcttt cgaaccgaga aggcactgag cttccgccca ccatggtggg
     1441 cgaccatggc caccgccaaa gcgatcgggt ccgcccccat ggtcatgccg ccgattgcga
     1501 ccacttccgg ttcgaggacc tcgagcacgg cccggcccac gatgtgagcc ccgtccccgc
     1561 tcatcgtcgt ccggcgggcg tcgaggtacc agtccgatcg cgcccctgat ctgagggtgt
     1621 aggggccgtc ggtgaacaat gcgtgtgtgc gcagatgatc gatcaaggct gaggagggca
     1681 cgccatcatc gtattgcccg accgatcgat atgagtcgtc ggggccggcg gtaccgttcg
     1741 ctcatggatc tcctcctcgt gcgtcacggg caacccgact ggaccccgga tcgcatcgcg
     1801 cgcaatgacc ccgacctgac caccctgggc catgagcaag caaaccgggt ggcgcagcga
     1861 ctcggcgagc tggagcgggt cgacgagctc tgggtgtcgt cgatgaaccg cgccctccag
     1921 acgtcggtcc cggtttcatc cgagatcgag atggtgcccg aggtccacgg ctggctacag
     1981 gagatcaaca acccaccttc ctgggacggc tcacccgccg acgagatcga gaaggcgctg
     2041 cgcgattcca acttccggac catggaggag atgtgggatg ggctgcccgg gggggagtcc
     2101 ttccgcgcct tccaccagcg ggtggtatcc ggtctcgagg ccaccctggc cgcgttgggg
     2161 atcacgccca tgcacggcga gcaccctcat ctctgggatg tgagcgaccc gaaccagaga
     2221 gtgatcgtcg tcgcccatgc cggcaccaac gccgtggtgc tgggtcgtct cctcggcctc
     2281 gagccggtgc cctgggagtg ggaccgcttt cgccagccac acaccggcgt ctcgcggctg
     2341 acgatgagtc
//
LOCUS       DATITW010000190         1678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_570235, whole genome shotgun sequence.
ACCESSION   DATITW010000190 DATITW010000000
VERSION     DATITW010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570235"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="VLS86_02645"
     CDS             complement(<1..1520)
                     /locus_tag="VLS86_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HSO49420.1"
                     /translation="MPATRGSGYEPIDEPIRAELFSVERLEEHGRSLAEAQELSERQG
                     RLRPISRRLVDNGEVLLSSYRELSKAIREEALLTPAAEWLVDNFHLVESQLHEIRDNL
                     PSSYYRELPKLDGGHLVGYPRVYGIVWAYVAHLDSRFDPEALRRFVASYQEVAPLTIG
                     ELWAIPITLRLILIENLRRMAESIIWARRLRREAEEIADGLLGIGGGRPGETRAALDR
                     YGPAKRPLPVAVQLLQRLRDQDPETTPGLTWLLDRLSAEGTSPDEIVGETHRRQAATN
                     VTVRNIVTSLRQVNFFDWADFVEGVSLVDHALAADSSYSEMSFATRDRYRHAIEELSK
                     GSGTSELDVTKTTLRLADPQLADRRQSDPGFYLLGEGRTTLEAQIGFRPGVGLVTSRF
                     VRRHAAAVYLSSVAVVSALILALPLLTVEQWSPWGWVMAGLALIPASDMGVVLVNRAI
                     AALTRPEELPSLALAHGPTAELRTLVAIPALLTRPDQVEELVERLEVHYLANPDGD"
BASE COUNT          306 a          521 c          557 g          294 t
ORIGIN      
        1 aaatcgccat ccggattggc caggtagtgg acctcgagcc gttcaaccag ctcctcgacc
       61 tggtcgggtc gcgtcaacag ggcagggatc gcaaccagcg tgcgcagctc tgcggtcggt
      121 ccgtgagcca acgccagtga aggcagctcc tcgggccggg tcagggctgc gatggcgcgg
      181 ttgacgagga ccacgcccat atcggaagcc gggatcaggg caaggccggc catcacccat
      241 ccccatggcg accactgctc gacggtgagc aggggcaaag cgaggatgag agcgctgacc
      301 acggcaaccg agctcaggta cacggcggcc gcgtgcctcc tgacgaatcg agacgtgacc
      361 agcccgacac cgggacggaa gccgatctga gcctcgagcg tggtcctgcc ctctccaagc
      421 aggtagaagc cgggatcgct ctggcgacga tcggcgagtt gagggtcggc cagtcggagt
      481 gtggtcttcg tcacgtcgag ctcggaggtc ccggatccct tcgacaactc ctcgatggcg
      541 tgccggtaac ggtcccgggt ggcgaagctc atctcgctgt aggagctgtc cgcggccaaa
      601 gcatgatcga cgaggctgac gccctccacg aagtcggccc agtcgaagaa gttgacctga
      661 cggaggctgg tcacgatgtt gcgaaccgtg acgttggtgg ccgcttgccg acgatgggtt
      721 tcgccgacta tctcgtcggg tgacgtcccc tcggcgctga gacgatccag cagccaggtg
      781 aggcccgggg tcgtctccgg gtcctggtcg cgaagacgct gcagcagctg gacagcaacg
      841 ggcagtggcc gcttggcggg gccgtagcgg tcgagggctg cccgtgtctc gccgggacgg
      901 ccgccgccga ttccgaggag cccgtcggcg atctcctcgg cctctcgacg aagcctcctg
      961 gcccaaatga tcgattccgc catccggcgc aggttctcga tcaggatcag gcgaagggtg
     1021 atggggatgg cccacagctc accgatggtt agaggcgcga cttcctggta cgaagccacg
     1081 aatctgcgca gcgcttcggg atcgaagcga ctgtccaggt gcgcgacgta tgcccatacg
     1141 atcccgtaaa cccgcgggta gcccaccaaa tgaccgccgt cgagcttggg aagctcgcgg
     1201 tagtaactgg aagggaggtt gtcccgtatc tcgtgcagct ggctctccac gagatgaaag
     1261 ttgtccacca gccattccgc cgcgggggtg agcagtgcct cttcccggat tgccttggac
     1321 aactcccggt atgaggagag aagaacctca ccgttgtcca ccagtcgccg ggagatgggg
     1381 cgcagtctcc cctggcgttc actcagttcc tgggcttcgg cgagactccg gccatgctcc
     1441 tccagccgtt cgacgctgaa cagctcggcc cggatgggct cgtcgatcgg ctcatatccc
     1501 gagcctcggg tggctggcac catcgcacaa tacgtgagca tccacaccgt gaactcgatt
     1561 ggcgatgtgt cgctgagaat tggtgctgac tcgaaatggg ccgctcgacg aagggacaga
     1621 cgaggtgacc caactcgatt gcgacaatcc ggttgttgct tctctcgtgt agctgtag
//
LOCUS       DATITW010000191         1808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_690510, whole genome shotgun sequence.
ACCESSION   DATITW010000191 DATITW010000000
VERSION     DATITW010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690510"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VLS86_02650"
     CDS             <1..203
                     /locus_tag="VLS86_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HSO49421.1"
                     /translation="GPWRAAPSLMVVVPTSEDVTIEFENTWAETGGLILSLAGIAVLV
                     GFGVAGLRRKKAGVSPDSVGSA"
     gene            complement(154..870)
                     /locus_tag="VLS86_02655"
     CDS             complement(154..870)
                     /locus_tag="VLS86_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSO49422.1"
                     /translation="MSPTLSIVVPVYNEAAFIPQALPLLISEMESLGLKYRIHLVENG
                     SNDGSAEVARSVAGQAPVTVSSLPTADYGAAMHHGFLEAEGEWVVNFDIDYFSARFLQ
                     QVMSTTDADLVIGSKRNPSSEDRRPLIRRLATWVFNLLLRTILGSKVSDTHGMKAFRR
                     NLVDDLAGLVVSRQDLYDTELVIRAERSGYRIIEVPVKVEEIRAARSSLVKRVPRTVV
                     GLMRIRRILRNQGSPQPSSA"
     gene            931..>1808
                     /gene="manB"
                     /locus_tag="VLS86_02660"
     CDS             931..>1808
                     /gene="manB"
                     /locus_tag="VLS86_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HSO49423.1"
                     /translation="MDSPIVFKAYDIRGRTDNGELTPELLEKVGGAFVSVVGAEQVAV
                     GRDCRISSPALFDGLVAGITGAGADVIDLGEVPTDLVYYYSGANPMPGAMITASHNPA
                     EYNGLKLCREMAAPVGADTGLNEIRDRVLSGIESSSATTGSVTKVEAVEAYVDHLLSI
                     VEPSSIGRMRVAVDGGNGMAGITVSRVFAKLDSVLDGLYLEPDGTFPNHPADPIQPEN
                     LVDLVEMVANGGHDLGVAFDGDADRAFFIDDQAQAVSGSTVTALIARWMLKRDPGAAI
                     IHNLITSRAVAEIIAEA"
BASE COUNT          341 a          545 c          584 g          338 t
ORIGIN      
        1 atgggccgtg gcgggcggcc ccgtccctga tggtggtggt ccccaccagc gaagacgtga
       61 ccatcgaatt cgagaacacc tgggcggaaa ccgggggttt gatcctgtct ctcgccggaa
      121 tcgccgtcct cgtcggtttc ggggtggccg gcctcaggcg gaagaaggct ggggtgagcc
      181 ctgattccgt aggatccgcc tgatccgcat caagccgacc acggtccggg ggacccgttt
      241 gaccaacgag gatctggccg cccgaatctc ctccaccttc accgggacct cgatgatccg
      301 gtatccgctt cgctcggccc ggatcaccaa ctctgtgtcg tacagatcct gtcgactgac
      361 gaccaggccc gcgaggtcat cgacgagatt gcgacggaag gccttcatgc catgggtatc
      421 cgacaccttc gagccgagga tcgtgcgcag gaggaggttg aagacccagg tcgccagacg
      481 acggatcagc ggccgtcggt cctcggagct cggattccgc ttggagccga tgaccaagtc
      541 ggcatccgtg gtcgacatca cttgctgcaa gaaccgggcg gagaagtagt cgatatcgaa
      601 attgaccacc cactcgcctt ccgcttccag aaatccgtgg tgcatggctg ctccgtagtc
      661 agcggtgggc aacgacgaga cggtcactgg cgcctgtccg gcgacggaac gcgccacttc
      721 cgccgatccg tcgttggagc cgttctcgac gagatgaatc cggtacttca gtcccagact
      781 ctccatttct gaaatgagca gcggcagagc ctgggggatg aatgccgcct cgttgtatac
      841 ggggacgacg atggagaggg ttgggctcat cggagtgggc gagtgtagga atcggagacc
      901 ggtacaccca cccctagaat cggcgtctcg atggactccc caatcgtctt caaggcctac
      961 gacatacgcg gccgcaccga caacggagaa ctgacaccgg aattgcttga gaaagtgggc
     1021 ggcgccttcg tgtcggtggt aggggcggaa caggtggcgg tagggcgtga ctgccgtatc
     1081 tcatccccgg ccctcttcga cggtctggtg gcgggaatca ccggtgcggg ggcggatgtg
     1141 atcgacctcg gggaagtccc caccgacctc gtctattact acagcggagc caacccgatg
     1201 ccgggggcga tgatcaccgc ttcgcacaac cctgccgagt acaacggtct caagttgtgc
     1261 cgcgagatgg ccgcaccggt cggggccgac accggtctca atgagatcag agatcgggtc
     1321 ctctccggga tcgaatcgtc gtcggcgacg accggctccg tgaccaaagt cgaggcagtc
     1381 gaggcctatg tcgatcatct cctctcgatc gtggagccct cctcgattgg gaggatgcgt
     1441 gttgcggtcg atgggggcaa cgggatggcc ggaatcacgg tgtcccgggt cttcgccaaa
     1501 ctcgacagtg tgcttgacgg tctctatttg gaaccggatg gcacgtttcc caatcatcct
     1561 gcagacccga ttcagcccga gaatctggtc gatctggtcg agatggttgc caacggcggt
     1621 cacgaccttg gcgttgcatt cgatggtgac gcagaccggg cgttcttcat cgacgatcag
     1681 gcccaggcag tgagcgggag cacggtgacc gccctcattg cccgatggat gctgaaacgc
     1741 gatcccggag cggcgatcat ccacaatctg atcacctcgc gggcggtggc cgagatcatc
     1801 gccgaggc
//
LOCUS       DATITW010000192         2842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_89707, whole genome shotgun sequence.
ACCESSION   DATITW010000192 DATITW010000000
VERSION     DATITW010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89707"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VLS86_02665"
     CDS             complement(<1..560)
                     /locus_tag="VLS86_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSO49424.1"
                     /translation="MSGRLEDKVALITGAGSGIGRETALLFAAEGASVLAVDLNQAAA
                     EETAEMARAGGGEAAAHHADVSSAEDSEAMVVAAETRFGRLDVMFNNAGIMHSDDDDA
                     VGTEEKVWDLTMDINAKGVFLGCKYGIPALRRAGGGSIINTASFVALLGAATPQVAYT
                     ASQGAVLAMTRELSIVHARESIRVNAL"
     gene            complement(557..1918)
                     /locus_tag="VLS86_02670"
     CDS             complement(557..1918)
                     /locus_tag="VLS86_02670"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HSO49425.1"
                     /translation="MISSEELEAGVASHDIETVVVAFTDHYGRLHGKRFAAHHFIEEI
                     SDHGTHGCDYLLTVDMEMEPVPGYTYANWELGYGDFHMVPDLSTLRLATWLDRTAFVL
                     CDLEDPKTHELVTVAPRTILRRQLERAAESGYTARGASELEYFLYQDSYQEAAEKRYQ
                     GIRPAGWYIEDYHLLQGTREEFYNAQVRRHLSASGIPVENSKGEWGRGQHEMNIRYTE
                     LLAMADRHTIMKQAMKEIADLSGVSITFMAKPDMTEAGSSCHLHVSLWSGDKSAFPGD
                     HVLGPVHGSDVFRWFLGGWMAHVPELMVFFAPTINSYKRYQDGSWAPTRLGWSYDNRT
                     AGFRVVGHDQSLRIECRIPGADANPYLAYAAVLAAGLDGIDNQLEPPDIFEGDVYQAR
                     ELPRVPYTLRDATDLFESSEFVNKVFGAAVQEHYSHFFRMEQKAFDNSVTDWERWRYF
                     ERI"
     gene            2089..>2842
                     /locus_tag="VLS86_02675"
     CDS             2089..>2842
                     /locus_tag="VLS86_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSO49426.1"
                     /translation="MSTERKVGSVTYRDVDQEYFDQRSLKRHAGVWMLWALGVAAVIS
                     GDFSGWNFGLDVGGFGGLLIATAVITVMYIGLCYSIAEMSPALPHTGGAYSFARSAMG
                     PWGGYITGLAENIEYVVTPAVVVYFAGAYAQPVFDDLFGLLIPQPAWWAIFYVIFVGL
                     NVIGVAATLRFSVVICLISLAVLAIFFVFAIPDFDAANLTNIAADPGNTSFLPFGVGG
                     IFAALPFAIWFYLAIEELPLAAEESMDPKRDVP"
BASE COUNT          485 a          907 c          899 g          551 t
ORIGIN      
        1 agggcgttga ccctgatgct ctcccgggca tggacgatgg agagctcacg ggtcatggcc
       61 agcaccgcgc cctgcgacgc ggtgtaggcg acctgtgggg tggcggcgcc gagcaaggcg
      121 acgaacgatg ccgtgttgat gatcgagccg ccgcccgccc gacgcagtgc gggaatcccg
      181 tacttgcacc cgaggaagac ccccttggcg ttgatgtcca tcgtcaggtc ccagaccttc
      241 tcctcggtgc cgaccgcatc gtcgtcatcg gagtgcatga tcccggcgtt gttgaacatc
      301 acatcgagac gaccgaatct ggtctcggct gccaccacca ttgcctcgct gtcctccgcc
      361 gaggagacat cggcatggtg ggcggctgct tcgccgcctc cggcccgggc catctccgcg
      421 gtctcctcgg ccgcggcttg attgaggtcg accgcgagca ccgacgcccc ctcggcggcg
      481 aagagcagag cggtctcgcg gccgatgcca gaacccgccc cggtgatcag ggccaccttg
      541 tcctcgagtc gaccgctcat atccgctcga agtatcgcca gcgctcccag tcggtcaccg
      601 agttgtcgaa cgccttctgc tccatgcgga agaagtgcga gtaatgctcc tgaaccgcgg
      661 cgccgaatac cttgttgacg aattccgacg actcgaagag atcggtggcg tcgcgcaggg
      721 tgtacgggac acggggaagc tcgcgggcct ggtagacgtc gccctcgaag atgtcaggtg
      781 gctcgagctg gttgtcgatc ccgtcgagac cggcggcgag gaccgcggcg taggccaagt
      841 aggggttggc atcggcgccc gggatccgac actcgatgcg aagactctgg tcatgaccga
      901 cgacgcggaa cccggcagtc cggttgtcat agctccagcc gagcctggtc ggcgcccagg
      961 agccgtcttg atatcgcttg taggagttga tcgtcggcgc aaagaagacc atcaactcgg
     1021 ggacatgggc catccagccg ccgagaaacc accggaagac atccgagcca tggaccggcc
     1081 ccagaacgtg gtcaccggga aaggcactct tgtcgcctga ccagaggctc acgtggagat
     1141 gacacgacga cccggcctcg gtcatatccg gcttggccat gaaggtgatg gagacaccgc
     1201 tgaggtcggc gatctccttc atcgcctgtt tcatgatcgt gtgccgatca gccatcgcca
     1261 acagctcggt gtatcggatg ttcatctcgt gctggccccg accccactca cccttggagt
     1321 tctccaccgg gatcccggat gcggacagat gacgtcgcac ctgggcgttg tagaactcct
     1381 cacgggttcc ctggagcagg tgatagtcct cgatgtacca gcccgcgggg cgtatcccct
     1441 gatacctctt ctcggcagcc tcctggtagg agtcctggta caggaagtac tccaactcgg
     1501 aggcgcctcg tgccgtgtac cccgactccg cggcacgctc cagctgacga cggagaatgg
     1561 tgcggggagc cacggtcacc aactcgtggg tcttgggatc ctcgagatcg cagaggacga
     1621 acgcagtgcg gtcgagccag gtggcgagac gaagcgtgga gaggtcgggg accatgtgaa
     1681 agtcgccgta ccccagctcc cagttggcgt aggtgtaccc gggaaccggc tccatctcca
     1741 tgtcgacggt cagcaggtag tcgcatccgt gcgtgccgtg atcagagatc tcctcgatga
     1801 aatggtgggc agcgaatcgc ttgccgtgca accgcccgta gtggtcggtg aacgccacga
     1861 ccacggtttc gatgtcatgt gaggcgacgc ccgcctcgag ctcttcgctg ctgatcatcc
     1921 ctcttacccc agccactgct tcgctcctcg gctctctgag ggcgcagaga gtaggcctct
     1981 gcacaccgat ccggctccaa cagaagcacc gagaaagggt gctactcaga cgtcatcggg
     2041 gagtgactgc tccctaatct ccgccggtat ccgaagggag gtacgtcaat gtcgaccgaa
     2101 cgcaaggtcg gctcggtcac ctatcgcgac gtcgatcagg agtacttcga tcagcgaagc
     2161 ctgaagcgcc acgccggggt atggatgctc tgggcgttgg gtgtggcggc tgtcatctca
     2221 ggtgacttct cgggctggaa cttcgggttg gatgtgggtg gcttcggggg cctcctcatc
     2281 gccaccgcgg tcatcacggt catgtacata ggtctctgct attcgatcgc ggagatgtcc
     2341 ccggccctgc cccacaccgg gggggcatac tcctttgccc ggtcggcgat ggggccgtgg
     2401 ggcgggtaca tcaccggttt ggcggagaac atcgaatacg tggtgacgcc cgccgtggtg
     2461 gtctacttcg ccggcgccta tgcccaaccg gtgttcgacg acctgtttgg cctgctgata
     2521 ccacaacccg cctggtgggc gatcttctac gtgatcttcg tcgggctcaa cgtcatcggc
     2581 gtcgcggcga ccttgcggtt ctcggtggtg atctgcctga tatcgctggc ggtgttggcg
     2641 atcttcttcg tcttcgccat ccccgatttc gatgccgcca acctgaccaa catcgccgcc
     2701 gaccctggca acacttcgtt cctccccttt ggcgtcggcg ggattttcgc cgctctgcct
     2761 ttcgctatct ggttctacct ggcgatcgag gagctgccgc tggccgccga ggagtcgatg
     2821 gatcccaagc gcgacgtccc ca
//
LOCUS       DATITW010000193         1531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_9651, whole genome shotgun sequence.
ACCESSION   DATITW010000193 DATITW010000000
VERSION     DATITW010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9651"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VLS86_02680"
     CDS             <1..574
                     /locus_tag="VLS86_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSO49427.1"
                     /translation="TDFTPFVRAMADFGAELIVATGHPPGNSAIVTLSADLHDVPVTG
                     AWTPPDLVVGGLQDLAIGRYTDFSCADYFSDSYADLAKRYLAFSDNKFMSDDAVAGYA
                     IVKIVAEAVGAVGDDPKAVAEYVHSHKFDMPGYAHPLSWTEWGELAEAQPIFTIISEG
                     PAPEGLKEAGDWWFELLTHSDPLEPYVPGS"
     gene            581..1321
                     /locus_tag="VLS86_02685"
     CDS             581..1321
                     /locus_tag="VLS86_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO49428.1"
                     /translation="MALLELDQVEKHFGGLAAVNGVTMRADEGEILAIVGPNGAGKST
                     LLKLIVGLEAPTRGTIRFAGEDITHHPPHRVRQMGVATVQQTPHHFSSMTVLENVALG
                     ALFGRAGAHRKEEQALELAGEALSFVGLADRRHDPVGNLNLHEQRFVELARAIAGQPR
                     LLVLDEVMAGLNDAELEASIEIIRMIRDQFNVTIIWVEHVMKAVMSLAERTVVLNFGD
                     VLTEGLPEDVMRHPAVVEAYLGKEIKHA"
     gene            1314..>1531
                     /locus_tag="VLS86_02690"
     CDS             1314..>1531
                     /locus_tag="VLS86_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO49429.1"
                     /translation="MLEVKGFSAGYQGNDVVHDVAFHVGPGEAVMIIGSNGAGKTTLF
                     RAMCGLLSPSSGKVLFEGDDITGMPAER"
BASE COUNT          287 a          457 c          488 g          299 t
ORIGIN      
        1 caccgacttc accccgttcg tacgggcgat ggccgacttc ggtgcggaat tgattgtggc
       61 cactggccat ccgccgggaa actcggcgat cgtcaccctg tcggcggacc tgcacgacgt
      121 gccggtcacc ggcgcctgga ctccgccgga tctggtggtc ggaggtctgc aggacctcgc
      181 catcgggcgc tacaccgatt tctcctgcgc cgattacttc agtgacagct acgccgatct
      241 ggccaagcgc tacctggcat tctccgacaa caagttcatg tcggacgatg ctgtcgccgg
      301 gtatgcgatc gtaaagatcg tcgccgaggc ggttggagcg gtcggtgatg acccgaaagc
      361 agtcgctgag tacgtccact cccacaagtt cgatatgcca ggctatgccc acccgttgag
      421 ctggaccgag tggggcgagc tggccgaggc ccagccgatc ttcaccatca tcagcgaagg
      481 accggctccc gaggggttga aggaagccgg cgactggtgg ttcgagttgc tgacccattc
      541 cgacccgctc gagccctacg tgccggggtc atagcgccaa gtggccctac tcgagttgga
      601 tcaggtcgag aagcactttg gaggcctcgc cgctgtcaac ggggtgacca tgagggctga
      661 cgagggcgag attctggcca tcgtcggccc caacggcgcc ggcaagagca ctctgctcaa
      721 gctgattgtc ggtctcgaag cgccgaccag aggcacgatc aggttcgccg gcgaggacat
      781 cacccatcac cctccccacc gcgttcgtca gatgggggtc gccacggtgc aacagactcc
      841 ccaccacttt tcctcgatga ccgtgctcga gaatgtggct ctcggggctc tctttggcag
      901 ggcgggagca catcgaaagg aagaacaggc tctcgagctc gccggtgagg cgctttcgtt
      961 cgtcggcttg gccgaccgac gccacgaccc cgtcggtaat ctcaacttgc acgagcagcg
     1021 gttcgttgaa ctggcccggg ccatcgccgg acaaccccgg cttctcgtac tcgatgaggt
     1081 gatggcaggt ctcaacgatg cagagttgga ggcctccatc gaaataatcc gaatgattcg
     1141 tgatcagttc aacgtgacca tcatctgggt agagcacgtc atgaaggcgg tgatgagtct
     1201 ggcggagcgg acggtggtcc tcaacttcgg ggatgtcctg accgaaggtc tgcccgaaga
     1261 tgtgatgcgc catccggcag tagtcgaggc ctatctgggc aaggagatca aacatgcttg
     1321 aggtcaaggg gttctcggcc ggataccagg gcaacgatgt cgtccacgac gtggcctttc
     1381 atgtcggccc aggtgaggcg gttatgatta tcggttctaa cggcgccggg aagacgacgc
     1441 tgttccgggc gatgtgcggg ctgctctcgc cttcttcagg taaggttctc ttcgagggtg
     1501 atgacatcac cgggatgcca gcggagcgca t
//
LOCUS       DATITW010000194         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_530395, whole genome shotgun sequence.
ACCESSION   DATITW010000194 DATITW010000000
VERSION     DATITW010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530395"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VLS86_02695"
     CDS             complement(<1..681)
                     /locus_tag="VLS86_02695"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HSO49430.1"
                     /translation="MSIENQLSELVDRLPAGITEGVALGTGLADGFDLYPSPVGEVAV
                     TFNVEGVSSVDLANVGFEQRFAERHGRRLIRAEPPAQWGRHIPDAIEAGTPGKVPVDL
                     SSRTPFQAQILRIAASIPRGEVRPYSWLAREASHPGAARAVGSTMARNPIPLIIPCHR
                     VVRSDGSIGEYSLGGPHNKWDLLTYEGVEPARLVELAEHHVRVQGNSSTGIYCHPTCR
                     AIRRSKQEN"
     gene            complement(678..1184)
                     /locus_tag="VLS86_02700"
     CDS             complement(678..1184)
                     /locus_tag="VLS86_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSO49431.1"
                     /translation="MTRSLAADLDGAFPTLVETTGPRLYWGLRRMCGDHQEAEDLTQE
                     TFIRAYRALVQFDRARFEELKLTPWLWTIALNLGRNHLRSRSRRPTLVEIGDSGVDDQ
                     ETPDSAAWDKRFTALTAPQRTAVVLRHVVGMGISEISQATGRPEGTIKADIHRGLERL
                     RRVMEQEA"
     gene            1249..1941
                     /locus_tag="VLS86_02705"
     CDS             1249..1941
                     /locus_tag="VLS86_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49432.1"
                     /translation="MKVMDESQIEAILTAAEGVVATGAPLGPTGFWKAVHVVKGDPSL
                     IERHADRIARIDRAALKAWALVTVPLWLGNVLMVGGSLFGLVLVAWAYSLEGTSAGLV
                     FLFGFGVVLVTTHGLGHLVAGRLMGIRFTHWFIGSISAPQPGVKVDYATYLRAPAPSR
                     AWMHASGAIVTKVLPFAFLGAALAADIPIWAVLILVAVGIGQIVTDLLWSTKASDWKK
                     FRREMGFAQSAS"
     gene            complement(1926..>2921)
                     /locus_tag="VLS86_02710"
     CDS             complement(1926..>2921)
                     /locus_tag="VLS86_02710"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSO49433.1"
                     /translation="FRRLLALVSIFAVTSSVVQAVLPGHTETLGGNAGTLGILLGAMG
                     AGALLGAFTRQQFLDRFRKRSLMTTITLFGLSGIGVGLATTVPIAFFFMFLSGIFWVL
                     TLTNLNATAQLMAPEWIRGRAMSLYSLAFGGILPIGAIIAGVLADVVGTGESMVILCS
                     GAVILGIFSPRFGIPTLDEVVSPEFSTGREAPIHDETVEGGPVLIFNTWKIDEADFDA
                     FTAVMNRVRLVRLRTGAYRWRLLRNVSDPLRLTELFAVGSWEEHLAQHRRIDDASAEV
                     IALARSFDRAEGPVTRHLIAVDVESPPNFDELVVAHEEMHQTDGSIPILADQEAD"
BASE COUNT          486 a          950 c          979 g          506 t
ORIGIN      
        1 gttctcctgc ttggagcgtc tgatggcacg acaggtcgga tgacagtaga tcccggtcga
       61 gctgttgccc tgaacccgca cgtggtgctc tgcgagctcc acgagacggg caggctccac
      121 gccttcgtag gtgagcaggt cccacttgtt gtggggaccg cccagcgagt actcaccgat
      181 cgacccgtcg gagcgaacca cccggtgaca ggggatgatc agcgggatcg ggttgcgcgc
      241 catcgtcgag cccacggctc gcgccgcacc cgggtggctc gcctcccggg ccagccagct
      301 gtacgggcgc acctcgccac gggggatcga cgccgctatc cgcaggatct gcgcctggaa
      361 aggggtcctc gagctcaggt cgaccgggac cttgcccggc gtccccgctt cgatcgcatc
      421 cgggatgtgt ctgccccact gcgccggcgg ttcggcccgg atcagacgcc ggccatggcg
      481 ctcggcgaac cgctgctcga agccgacgtt ggcgagatcc accgaggaga cgccctccac
      541 gttgaaggtg accgctacct cgcccacagg ggacgggtag aggtcgaacc cgtctgcgag
      601 cccggtgccc agggcaaccc cctcagtgat cccggccgga agccggtcga ccagctctga
      661 cagttgattc tcgatgctca tgcttcctgc tccatcactc gtctcaatcg ttccagtccg
      721 cgatggatgt ccgccttgat cgtcccctcc ggtcggccgg ttgcctggga gatctccgag
      781 atccccatcc cgaccacgtg tcggagcaca acggccgtcc gctgtggggc ggtcagggcg
      841 gtgaacctct tgtcccacgc cgccgagtcg ggcgtctcct ggtcgtcgac gccggagtcg
      901 ccgatctcga ccagggtcgg ccggcgcgac cggctgcgga ggtggtttct ccccaggttc
      961 agcgcgatgg tccacagcca gggggtcagc ttcagttcct cgaatctggc ccgatcgaac
     1021 tggacgaggg cccggtaggc gcggatgaag gtctcctgag tcagatcttc ggcctcctgg
     1081 tggtctccgc acattcggcg caagccccag tacagccgcg ggccggtggt ctccaccagg
     1141 gtggggaacg caccgtcgag gtcggcggcc agggatctgg tcatttcatc catcgcgagg
     1201 agaacaccgc ctggcatgat ccggttgcag agacggcacc gtacttagat gaaggtgatg
     1261 gacgagagtc agatcgaggc gattctcaca gccgccgagg gcgtggtggc caccggcgcg
     1321 ccgctgggcc cgaccgggtt ctggaaggcg gtccatgtgg tcaagggcga cccgtccctg
     1381 atcgagaggc acgcggatcg gatcgcacgc atcgatcgcg cagccctcaa ggcgtgggct
     1441 ctggtgacgg tgcccctgtg gctgggcaac gttctcatgg tcggcggctc gttgttcggg
     1501 ttggtgctcg tggcctgggc ctattcgctc gaaggcacca gcgcagggct cgtcttcctg
     1561 ttcgggttcg gcgttgtcct ggtcaccacc catgggctcg gccatctggt cgcgggccgg
     1621 ctgatgggga ttcggttcac ccactggttc atcggctcca tctcggctcc ccaacccggg
     1681 gtcaaggtcg actacgccac ctatctccgg gctccggcgc cgagcagggc ctggatgcat
     1741 gcctcaggcg cgattgtgac caaggtcctt ccctttgcct tcctcggcgc ggcgttggcc
     1801 gccgacatcc cgatttgggc tgtgttgatc ctggtcgcgg tcgggattgg ccagatcgtc
     1861 accgatctcc tgtggtcgac caaagcctcg gactggaaga agttccgcag agagatgggt
     1921 ttcgctcagt ctgcttcctg atcggcgagg atcgggatcg agccgtccgt ctgatgcatc
     1981 tcctcgtggg cgaccaccaa ctcatcgaag ttgggtgggc tctccacatc caccgcgatc
     2041 aggtgtcggg tcaccggccc ctcggcccgg tcgaaggatc gggccagggc gatcacttcc
     2101 gctgaggcgt cgtcgattcg gcgatgctgg gcaagatgct cctcccagga gcccacggcg
     2161 aacaactcgg tgagacgcag cgggtcggag acgttgcgaa ggagccgcca tcgataggcg
     2221 ccggtgcgca atcgaaccag ccgaacccgg ttcatcacgg cggtgaaggc gtcgaagtcg
     2281 gcttcgtcga tcttccaggt gttgaagatg agcaccggcc ccccctcgac ggtctcatcg
     2341 tggatcggag cctcccgacc ggtcgagaac tcgggggaga ccacctcgtc gagggtagga
     2401 atgccgaacc ggggcgagaa gatgcccaat atcaccgcac cggagcagag aatgaccatg
     2461 gattcgccgg tccccaccac atcggcgagg accccggcga tgatggcgcc aatggggagg
     2521 atgcccccga acgccaggct gtacaacgac atcgcccgtc ccctgatcca ttccggggcc
     2581 atcagttggg cggtcgcgtt caggttggtc agggtcagca cccagaagat cccgctgagg
     2641 aacatgaaga agaaggcaat ggggacggtg gtggcgagac cgacaccgat gcccgagagc
     2701 ccgaagaggg tgatcgtcgt catcagcgag cgcttccgga agcgatcgag gaactgctgc
     2761 cgtgtgaatg ccccgagcag cgccccggcg cccatggccc cgagcaggat ccccagggtc
     2821 ccggcgttac cgcccagagt ctcggtgtga cccgggagca cggcctggac caccgacgag
     2881 gtgacggcaa agatcgagac cagggccagc agtcgacgga a
//
LOCUS       DATITW010000195         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_570554, whole genome shotgun sequence.
ACCESSION   DATITW010000195 DATITW010000000
VERSION     DATITW010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570554"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VLS86_02715"
     CDS             <1..753
                     /locus_tag="VLS86_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSO49434.1"
                     /translation="QAMIRQAAEVDVRAVLPNIQAPVLVMHRTGYQGFPMALSRYVAE
                     HIPNGRFLELPGSDGPPWFEHADLFIEATRDFAQGLVGDAAHRTRAERVMATVLFTDL
                     VSSTERAQQVGDSEWRNLLQLHEDVSRRCVGDTGGKVVKTTGDGILATFDVPGRAISC
                     ATTLSRNLERLGLPIRAGIHTGEIEVRDDDVGGIAVHIASRVMAAAAPAEVLVSRTVR
                     DLVVGSDFVFSDRGQHSLKGIDGEWQLFALAN"
     gene            941..1483
                     /locus_tag="VLS86_02720"
     CDS             941..1483
                     /locus_tag="VLS86_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49435.1"
                     /translation="MDDDESNVAIPGLAAADEVPLVATEPDPSTDSFTWSDAYVVEDL
                     PKINVGQPTAEFMEDAARDPDEASQLVCMGDASGSDFCGVIASTEPDIAGVTFGERDV
                     RAWSWNNVPEEAAAVQFRDQDGTQTWQRPADSLVRFVMFPDTIPDDPDGLCPCRFDAI
                     DSDGEVITSVDIRTGEYVDS"
     gene            complement(1544..>2601)
                     /locus_tag="VLS86_02725"
     CDS             complement(1544..>2601)
                     /locus_tag="VLS86_02725"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSO49436.1"
                     /translation="LIPWVYTAAVSVSLAVFAVTHRYRWFRFTQLFLILTLPFLLQLA
                     LGGFVNASAVIIWSLLAPLGALAISGRPQAILWFAGYAGLVVAAQLIQPSLDIDNNLP
                     QPLVAVFFVMNIVATSGVAFFALHYFVGQKDQAFEMLDLEREKSDRLLLNVLPKEIAE
                     LLKENGQTIAARCPAITVLFADVVGFTPLSQGLSPEEVVNLLNDVFSFFDSLVDKYGL
                     EKIRTIGDSYMVASGVPLPRDDHAHALARMALEMTGYVPSARSPGTPPIVFRLGISSG
                     PAVAGVIGKAKFQYDVWGDTVNTASRMESHGVAGRIQLSASTYELIKDDFVVEPRGVV
                     ELKGNGPMETWFLVGAR"
BASE COUNT          516 a          799 c          840 g          446 t
ORIGIN      
        1 caggcgatga tcaggcaggc ggccgaagtg gacgtgcgcg cggtcctgcc caacatccag
       61 gcccctgttc tggtgatgca caggactggc tatcaagggt ttccgatggc attgagtcga
      121 tacgtggcgg agcacatccc caacggcagg ttcctcgagc ttccgggaag cgatggccct
      181 ccatggttcg agcacgcaga tctattcatc gaggctacga gagacttcgc ccaagggttg
      241 gtcggtgacg ccgcacaccg gacgcgagcc gaacgggtga tggccacggt gctcttcacc
      301 gacctggtgt cgtccacgga gcgggcccag caggtcggag acagcgagtg gaggaacctg
      361 ctccaactgc acgaggacgt ctctcgccgt tgcgtcggcg acaccggcgg gaaggtcgtg
      421 aagaccaccg gtgacggaat cctcgccacc ttcgacgtcc ctggccgggc gatctcttgt
      481 gccaccacct tgagccggaa cctggagcgt ttggggctcc cgattcgggc cggaatccac
      541 accggcgaga tcgaagtgcg tgacgacgac gtcggcggga tcgcagtcca tatcgcatcg
      601 cgggtgatgg cggctgccgc acctgccgag gtcctggtgt cgcggacggt tcgtgacctg
      661 gtggtcggct ccgatttcgt cttctccgat cgaggacaac actccctcaa gggcatcgac
      721 ggtgagtggc agctgttcgc cctggcgaac tgagcaccgg gattcctcca acctcgagtc
      781 cttcggaggg ccctcgacgg aaccacccag atcggtagcc tccgggtgca tagctcggag
      841 gtgtccagcg aggagtgttc cgaacggaag aggcgggcat cctggcttcc tcctcattgc
      901 ggtactggcc ctactcgtcg caggttgtgt tggcgacctc gtggatgacg acgaatcgaa
      961 tgtcgccatc ccggggctgg ccgcagccga cgaggtccca ctggtcgcaa ccgaaccgga
     1021 tcccagcacc gactcgttca cctggtccga cgcctacgtg gtcgaagacc tgcccaagat
     1081 caacgtaggc caacccaccg ctgagttcat ggaagacgct gcccgtgatc ctgacgaagc
     1141 cagccagctc gtctgcatgg gagacgccag cggcagtgac ttctgtggtg tgatcgccag
     1201 tacggagccg gacatcgcag gggtcacatt tggcgaacgt gacgttcgtg cctggtcctg
     1261 gaacaacgtg cccgaagagg cagcagcggt ccagttcaga gaccaggacg gcacccagac
     1321 ctggcaacgg cccgccgaca gtctcgtgcg tttcgtgatg ttccccgaca ccatccctga
     1381 tgaccctgat gggctttgcc cttgtcgctt cgatgccatc gactccgacg gcgaagtgat
     1441 cacctcggtg gacatccgaa ccggggaata cgtcgacagc taggtgcttc cccaagaccc
     1501 ggggcccagc gcccaggtca tctcgaggag gatgggctca ggttcatctg gccccgacca
     1561 gaaaccaggt ctccatcggg ccattaccct tgagctccac caccccgcgc ggctcaacca
     1621 cgaagtcgtc cttgatcagc tcataggtgg aggcactgag ctggatccgc ccggcgacac
     1681 catgggattc cattcggctc gcggtattga ccgtgtcgcc ccagacgtcg tattggaact
     1741 tggccttccc gatgactccg gcaaccgccg gccccgagct gatgccgagt ctgaacacaa
     1801 tcggtggcgt gccaggcgaa cgagccgagg gcacgtaccc ggtcatctcc aaggccattc
     1861 gggccagggc atgagcgtgg tcgtcccgtg gcagcggcac ccccgaggcc accatgtagc
     1921 tgtcgccgat ggtccggatc ttctccagac cgtatttgtc gaccagggag tcgaagaaag
     1981 agaacacgtc gttgaggaga ttcaccacct cttcgggcga gaggccttgc gacagcgggg
     2041 tgaaaccgac gacgtcggca aagagcacgg ttatcgcagg gcaacgggcg gcgatggtct
     2101 gaccgttctc cttgagcagt tcggcgatct ccttggggag gacgttgagc aagagacggt
     2161 ccgatttctc acgctccagg tcgagcatct cgaacgcctg gtccttctga ccgacgaagt
     2221 agtgcagggc gaaaaaggcc acacccgagg tggcgacgat gttcatcacg aagaagaccg
     2281 ccaccagtgg ctgaggaagg ttgttgtcga tgtcgagcga aggctggatc aactgagcgg
     2341 cgaccaccaa gcccgcgtag cccgcgaacc agaggatggc ttgaggtcgg cccgagatcg
     2401 ccagcgcacc cagcggggcg agcagcgacc agatgatcac ggcactggcg ttgacaaagc
     2461 caccaagcgc gagctggagg aggaacggca gggtgaggat gaggaacaac tgggtgaatc
     2521 ggaaccaccg gtagcgatgg gtgacggcga agacagccag gctgacagat accgctgcgg
     2581 tatagaccca tgggatgagt g
//
LOCUS       DATITW010000196         3334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_49896, whole genome shotgun sequence.
ACCESSION   DATITW010000196 DATITW010000000
VERSION     DATITW010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49896"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..2843
                     /locus_tag="VLS86_02730"
     CDS             75..2843
                     /locus_tag="VLS86_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HSO49437.1"
                     /translation="MSLTDELNRLEEARKGEVAWKKWGPYLSERQWGTVREDYSEGGD
                     AWNFFTHDHSRSRAYRWGEDGLAGISDEKMHLCFALALWNGKDPILKERLFGLTNSEG
                     NHGEDVKEYYFYLDSTPTHSYMKYLYKYPQAAFPYADLAETNRRRTRDEMEYELLDTG
                     VFDADRYFDIFVEYAKAGPEDILVKITAANRGPDAAELHLLPTLWFRNDWAPWIAEVS
                     RAAHKPNLRQIDAAAGTSTAAATDPALGEYTLCCEGEVPLLFTENETNHERLFPGQKN
                     EDPYVKDGINDCVVQGNPGAVNPEKEGTKVAAHYRRVVGSGQSATVRLRLTGQAAAGK
                     SGKAKTTSVFFETDFEKTLAVRLQEADAFYRSVTPPSASPDEANVMRQAIAGMLWSKQ
                     FFFFDGDNWLDEHKSNPLHAGYRISRNSEWFHMVNEDIISMPDKWEYPWYAAWDLAFH
                     TLPLAIVDPDFAKEQMGLMLRSRYLHPSGQMPAYEWNFSDVNPPVHAFATLFLYRTEQ
                     ALRGEADVDFLKSSFNKLLLNFTWWVNRKDRFGKNVFEGGFLGLDNIGVFDRSAPLPT
                     GGHLEQADGTAWMALFSQNMLELAAELAVHDPIYEDMVFKFSEHFFFIAAAMNRPGQD
                     SMWDEEDGFYYDLLRLPDGSATRLKVRSMVGLLPLCATTVVEAWQRERVPRAMAGIVE
                     RWRRMPELQKAIHPTGPGHQGVEGRGIFALLNPERLRRILTKMLDENEFLSPCGIRSI
                     SRFHERHPYVFHVNGQEYRVDYLPAESNTGMFGGNSNWRGPVWMPVNALILRALLNFY
                     LYYGDNFKIECPTGSGKLMNLFEVSKEIADRLGRIFLRDGQGRRPVHGGTEKFQTDPH
                     WRDLILFYEYFHGDNGAGLGASHQTGWTGFVAKTIQLFGLLDPKRALEVGKRSGFAQG
                     NRGPGKEK"
     gene            2848..>3334
                     /locus_tag="VLS86_02735"
     CDS             2848..>3334
                     /locus_tag="VLS86_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HSO49438.1"
                     /translation="MRAWPRHPVIYEINTWVWLSDLSRKYRKVVDLATVPEQEWDAVA
                     SHGFDAVWFMGVWERSPAGVEISMRNTGLLEDFRRALPDFSAEDNVGSPYCVRRYVVD
                     EHLGGPIGLATARNTLAKRGIGLILDFVPNHVAPDHPWVVAHPEYFVQGSPDDARNDP
                     AS"
BASE COUNT          648 a         1081 c         1057 g          548 t
ORIGIN      
        1 gctttcaatg ccgggcttcc tgagctcagg atagcgtaat gggtgccggt acctcaagag
       61 aggggggtgg aaaaatgtcc ctgaccgacg aactgaatcg actcgaggaa gcccgcaagg
      121 gggaggtcgc atggaagaaa tggggtccct acctcagcga gcggcagtgg gggacggtcc
      181 gggaggatta cagcgagggg ggcgatgcct ggaacttctt cacacacgat cattcccgtt
      241 cccgtgctta tcgctggggc gaagacgggc tggccggcat ttccgatgag aagatgcacc
      301 tgtgcttcgc gctggccctg tggaacggga aggacccgat tctcaaggaa cggctcttcg
      361 gcctgaccaa cagcgaaggg aaccacggcg aggacgtcaa ggagtactac ttctacctcg
      421 acagcacgcc gacccactcc tacatgaagt atctctacaa gtacccgcag gcggcctttc
      481 cctacgccga cctggcggag acgaaccgcc gtcgtacccg ggacgagatg gagtatgaac
      541 tcctcgacac cggcgtcttc gacgccgatc gctatttcga catcttcgtg gagtacgcca
      601 aagccggccc ggaagacatc ctggtgaaga tcaccgcagc caaccgggga ccggacgcgg
      661 cggaactgca cctcctgccg acgctctggt tccgcaacga ctgggcgccg tggatcgccg
      721 aggtcagcag ggccgcccat aaaccgaacc tccggcagat cgacgcggcg gcgggcacaa
      781 gcacggctgc agcaacggat ccggcgctgg gggagtacac cctctgctgc gaaggcgagg
      841 tgccgctcct cttcaccgaa aacgaaacga accacgagcg actcttcccc ggacaaaaga
      901 acgaggaccc ctacgtcaag gacggcatca acgactgcgt ggtacagggg aatccggggg
      961 cggtgaatcc cgaaaaggag ggcaccaagg tcgcggcgca ctaccgacgg gtggtgggtt
     1021 ccggccagtc ggcgaccgtg cggttgcgcc tgaccggcca ggccgccgcc ggcaagagcg
     1081 ggaaagccaa aaccacctct gtcttcttcg aaacggactt tgagaagacc ctggccgtcc
     1141 ggctgcagga ggcagacgcg ttctaccgct cggtgacccc gccttccgcc tccccggatg
     1201 aagccaacgt gatgcgccag gccatcgccg gcatgctgtg gagcaagcag ttcttcttct
     1261 tcgacggaga caactggctg gacgagcaca aatccaaccc cctccatgcc ggatatcgca
     1321 tctcccggaa ctcggagtgg ttccacatgg tcaacgagga catcatctcg atgcccgaca
     1381 agtgggagta cccctggtac gcggcctggg acctggcatt ccacacgctc ccgctggcga
     1441 tcgtggaccc cgacttcgcc aaggagcaga tggggctgat gctccgcagc cgttatctgc
     1501 accccagcgg ccagatgccc gcctacgagt ggaacttcag cgacgtgaac cccccggtcc
     1561 acgccttcgc gacgctcttc ctgtaccgga ccgagcaggc cctgcgcgga gaggcggacg
     1621 tggatttcct caagtcgtcc ttcaacaagc tgctgctgaa cttcacctgg tgggtgaacc
     1681 gcaaggaccg gttcggcaag aacgtcttcg aggggggctt cctcggcctc gacaacatcg
     1741 gcgtcttcga ccgcagcgcc ccgcttccca ccggcggcca cctggagcag gcggacggca
     1801 cggcgtggat ggccctcttc agccagaaca tgctggagct cgcggccgag ctcgccgtcc
     1861 acgaccccat ctacgaggac atggtcttca agttctccga gcactttttc ttcatcgcgg
     1921 ccgccatgaa ccgtccgggt caggacagca tgtgggacga ggaggacggc ttctactacg
     1981 acctgctccg gctccccgac ggcagcgcca cgcggcttaa agttcgctcg atggtggggc
     2041 tcctgcccct gtgcgcgacg accgtggtcg aggcgtggca gcgggagcgc gtcccgcggg
     2101 cgatggccgg gatcgtggaa cgctggcgcc ggatgcccga gctccagaag gccatccatc
     2161 ccactggccc gggacaccag ggcgtggagg gccgggggat cttcgcactg ctcaacccgg
     2221 agcggctccg ccggattctc acgaagatgc tcgacgagaa cgagttcctg agcccctgcg
     2281 gcatccgctc gatttcccgg ttccacgagc ggcacccgta cgtcttccac gtgaacggcc
     2341 aggagtaccg ggtggactac ctgccggccg agtcgaacac cgggatgttc ggcggcaact
     2401 ccaactggcg gggcccggtc tggatgccgg tgaacgcact catccttcgg gcactgctga
     2461 acttctacct gtactacggc gacaacttca agatcgagtg ccccacgggc tccggcaagc
     2521 tgatgaacct cttcgaggtg agcaaggaga tcgccgaccg gcttggccgg atctttctgc
     2581 gcgacgggca gggccggcga cccgtccatg gcggcacgga gaaattccag accgatcccc
     2641 actggcgcga cctgatcctc ttttacgaat acttccacgg cgacaacggc gccgggctgg
     2701 gcgcaagcca ccagaccggc tggaccgggt tcgtggccaa aaccatccag cttttcgggc
     2761 tcctagatcc caagagggcg ctggaggtgg gcaagcggtc cgggttcgct cagggcaacc
     2821 gcggccccgg gaaggagaag tgacgagatg agagcgtggc ccaggcatcc ggtcatatac
     2881 gagatcaaca cgtgggtgtg gctgagtgac ctgagccgga aataccggaa ggtcgtggac
     2941 ctcgccacgg ttccggagca ggagtgggat gccgtcgcct cccatggctt tgacgccgtc
     3001 tggttcatgg gggtgtggga gaggagcccg gccggggtcg aaatctccat gcgcaacacg
     3061 gggctcctcg aggatttccg gagagccctc cccgattttt cagcggagga caatgtcggc
     3121 tccccctact gcgtgcgccg ctacgtcgtc gacgaacacc tcggaggccc gattgggctc
     3181 gcaacagccc gtaacactct cgcaaagcgc ggcattggtc tcatcctcga ttttgtgccg
     3241 aaccacgtcg ctcccgacca cccctgggtc gtcgctcatc cggaatactt cgtccagggg
     3301 agccccgacg atgcgaggaa cgatccggca tcct
//
LOCUS       DATITW010000197         3697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_290167, whole genome shotgun sequence.
ACCESSION   DATITW010000197 DATITW010000000
VERSION     DATITW010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290167"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..774
                     /locus_tag="VLS86_02740"
     CDS             76..774
                     /locus_tag="VLS86_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016460401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSO49439.1"
                     /translation="MTVPTDRAIILVVDDEEALSDLGSSALRFAGYTVRVENNGFDAL
                     RSIKNETPDLVVLDVNMPEIDGFEVCRRIRRDGIQVPVIFLTARDDIDDLRVGFRQGG
                     DDYLTKPFSLEELGLRIEALLRRGAGNVEPPTLTVGDLLIDDAAHQVFRGELEIRLSP
                     TEFRLLRFLILNRGRVMSKSQIVDHVWEYDFDGNYGIVETYISYLRKKLGDDDSSLLQ
                     TVRGVGYVIRGPKV"
     gene            778..2190
                     /locus_tag="VLS86_02745"
     CDS             778..2190
                     /locus_tag="VLS86_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSO49440.1"
                     /translation="MSLRTRLVVSFTVLLLAVIVAVGLVASRSIETILIEQTDRVLIG
                     FVERGPIPGFPMPAPGSEGETDDEEILRTISEVVVTEDGNVLFSRPSGFSDDPDPLPR
                     VTDLPDTSDPFFVQSVDGSLEYRAVAVVLSEELEMLNLPEGLTLVRAAPLTDVEAATE
                     SLVQALVLAGFVVLIVGGAVTWWAVDRSMRPVEQMVETAEAIASGDLSRRVPELEKGT
                     ELGRLGASLNEMLAHIEEAVDTEREGRERLRQFIGDASHELRTPLTAISGYAELRRKG
                     GLTTPEEEAKAWSRIESEGNRMGSLIEDLLVLTRLGQSQPLEITEFDLVQIARNAAAD
                     HQVIDHERPVAVVGAETAMINADEQRLHQVVTSLLDNVRVHTPVGTAVQIEIRDEGET
                     VVLEVSDNGPGIPESALGHIFDRFYRADPSRARSSGGSGLGLSIVEAIVIAHGGTVAA
                     SNLDGGGALIGITLPRVSAP"
     gene            2285..2902
                     /locus_tag="VLS86_02750"
     CDS             2285..2902
                     /locus_tag="VLS86_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49441.1"
                     /translation="MRTRALLLLLAAGLAACGGAAADGGDEVASLSDTSTTAPVEEAL
                     DSEAAAIAFTECMRENGVEMEDPTVDADGNVVPGLPTDLPDPEDGDEAVRVDGALGEE
                     MRGAFEECGDLLEGTAFGFTNGDVTELQDELLDLAQCLRDQGLDVADPDLSAGANEGA
                     GPGAGPFGIDFEDPEVQAALEVCEEFLPNLGGPAGEIRPPGDSGE"
     gene            2965..>3697
                     /locus_tag="VLS86_02755"
     CDS             2965..>3697
                     /locus_tag="VLS86_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49442.1"
                     /translation="MKILDETPREPRSRSKRWWLLGLALALVGAAGAWYLLDDASMAV
                     VDPESTIELQFSEVVTTDLLETSEYEGTMGRLDGNPVSIRREGTVTALPEEGATLEQG
                     DVVAWIDNQPVIVLYGELPMWRTMSDDVEGPDVLQLETALTALGFNESESSMTVDDTY
                     TGSTESVGETWQESIGADEDGTVDLGDVIFLPGPVRVDALQVSVGDQVASGTAIFTTS
                     GDDLEIDFDLPTSEQDNVSVGDAVEV"
BASE COUNT          713 a         1151 c         1183 g          650 t
ORIGIN      
        1 cgcgccgtcc tgctctgagg ctgttcccaa ctatctcaca gacaactcac aggaaacggg
       61 gatagcttcg taatgatgac cgtccccacc gacagggcca tcatcctggt cgtcgacgac
      121 gaggaggccc tgtccgatct cgggtccagc gcacttcgtt ttgccggcta caccgtccgg
      181 gtcgagaaca atgggttcga cgccctgcgc agcatcaaga acgagacacc agacctcgtc
      241 gtgctcgacg tgaacatgcc cgagatcgac ggattcgagg tttgccgtcg gattcgccgg
      301 gacgggatcc aggtgcctgt gatcttcctg accgcccgcg atgacatcga cgatctgcgc
      361 gttggctttc gccagggtgg cgacgactat ctgaccaagc cgttcagtct cgaagagctg
      421 gggctgcgaa tcgaggccct gctgagacgt ggcgccggca acgtcgaacc gcccaccctc
      481 accgtcggcg accttctcat cgacgacgcc gcgcatcagg tgttccgggg cgagttggag
      541 atacgactct ctccgaccga gttccgtctt ctccggtttc tgatactcaa ccggggacgc
      601 gtcatgtcga agagccagat cgtggaccat gtctgggagt acgacttcga cggcaactac
      661 ggcatcgtcg agacctacat ctcctatctc cgaaagaagc tgggcgatga cgactcatca
      721 ctgctgcaga ccgtccgcgg cgtcggctat gtgatccgcg gtcccaaggt ctgatggatg
      781 agtctgcgca ccagactcgt ggtgtccttc actgtcctct tgttggcggt gatcgtggcc
      841 gtaggcctgg tcgcatcgcg ttcgatcgag accatcctca tcgagcagac cgaccgggtc
      901 ctgatcgggt tcgtggaaag ggggcccatc ccgggcttcc cgatgccggc tcccggttcc
      961 gagggcgaaa ctgacgatga ggagatactg agaaccatct ccgaggtggt ggtcacggaa
     1021 gacggcaacg tcctcttctc ccgcccttcg ggcttcagcg acgaccccga cccactgccc
     1081 agggtgaccg acctgcccga caccagtgat cccttcttcg tccagtcggt cgacgggagt
     1141 ctcgagtatc gggcagtcgc cgtggttctc tccgaggagc tggagatgct gaatctgccg
     1201 gagggattga cgctggtcag agctgccccc ctcaccgatg tcgaagcagc caccgagagc
     1261 ctggttcagg cccttgtgct tgccgggttc gtggtgctca tagtcggcgg tgccgtcacc
     1321 tggtgggcgg tcgaccgctc gatgcgaccg gttgagcaaa tggtcgagac cgccgaagcc
     1381 atcgcgtctg gagacctctc gagacgtgtt ccagaactgg aaaagggaac cgaactgggt
     1441 cggctcggcg cctcgctcaa cgagatgctg gcccatatcg aagaggcggt ggacaccgag
     1501 cgagaaggac gggagcgtct gcgccagttc atcggcgatg cctcccacga gctgcgcaca
     1561 ccactcactg cgatctcggg atacgcggag ctgagacgaa aaggcggtct caccactccg
     1621 gaggaagaag ccaaagcgtg gtcgaggatc gagagcgagg gcaaccggat gggctctctg
     1681 atcgaagact tgctcgtgct cacccggctc ggacagagcc agccgctcga gatcaccgag
     1741 ttcgatctgg ttcagatcgc acgcaacgct gcggctgatc atcaggtaat cgaccacgag
     1801 cgcccggttg cggtggttgg tgcagaaaca gccatgatca acgccgacga gcaacgactg
     1861 catcaggtag taaccagtct gctggacaac gtgagggttc acaccccggt cggaaccgcg
     1921 gttcagatcg agatcagaga cgagggcgaa accgtcgtcc tcgaggtgag cgacaacggt
     1981 ccgggcatac ccgaatcggc gctgggtcac atcttcgacc gtttctaccg ggccgatccc
     2041 tcccgagccc ggagctccgg agggagcgga ctgggtctct ccatcgtcga agcgatagtc
     2101 atcgctcacg gtggaactgt cgccgcgtcc aatctcgacg ggggaggggc actcatcggg
     2161 atcaccctcc cccgggtctc cgccccctga accagcttct gaggtaactc acaggtcgcc
     2221 cccagggcgg tcacaggccg gtcggcgatc ctgatgccaa agccttccaa ggagcatcca
     2281 actgatgaga acaagagcac ttctattact gctcgccgcc ggtctcgccg cgtgtggggg
     2341 agctgccgcg gacgggggcg acgaggtcgc cagcctcagc gatacctcta ccactgcgcc
     2401 cgtcgaggag gcactcgact ccgaggcagc cgcaatcgcc ttcacggagt gcatgcgcga
     2461 gaacggcgtg gagatggagg acccgacagt cgacgcagac gggaacgtgg tgccgggtct
     2521 tcctaccgac ctcccggacc ccgaagacgg ggatgaggcc gttcgcgtcg acggcgccct
     2581 gggtgaagag atgcgagggg cgttcgagga gtgcggcgat ctgctcgagg gaaccgcgtt
     2641 tggattcacc aatggcgacg tgaccgagct ccaggatgag ctcctggatc tggctcagtg
     2701 tctccgcgac caggggctcg acgtcgccga ccccgacctg tccgccgggg cgaatgaggg
     2761 cgccggacct ggagccgggc cattcggaat cgacttcgaa gatcccgagg tgcaggccgc
     2821 cctcgaggtg tgcgaagagt tcctgcccaa cctcggtggt cccgccggcg agattcgccc
     2881 gcccggcgac tccggcgaat gaacgttccc gacttgaaag gacccatcga catggcagcg
     2941 acagacacac gtgagaccaa aaaggtgaag atcctcgatg agacgccgcg ggagccgcgg
     3001 tcgcgatcga aacgctggtg gctcctcgga ttggcactgg cattggtggg ggcggccggc
     3061 gcttggtacc tgctcgacga cgcgagcatg gccgtggtcg accccgaaag cacgatcgag
     3121 ctccaattct ccgaggtggt gactactgat ctcctcgaaa cctccgaata cgagggcacg
     3181 atggggcgtc tcgacgggaa cccagtgagc atccgccgtg agggaaccgt cactgccctg
     3241 cccgaggaag gcgcaactct cgagcaaggc gacgtcgtgg cctggatcga caaccagccg
     3301 gtgatcgtgc tctatggcga gttgccgatg tggcggacga tgtccgatga cgtcgaggga
     3361 cctgatgtgc tccagctgga gactgccctt accgccctcg gattcaacga gtctgaatcc
     3421 tcgatgaccg tcgacgacac ctataccgga tcgaccgagt cagtgggcga gacctggcag
     3481 gagagcatcg gcgccgacga ggacggaact gtcgatctcg gcgacgtcat cttcctgccg
     3541 ggcccggtgc gtgtagatgc tcttcaagtc agcgtcgggg accaggtagc gtccggaacc
     3601 gccatattca ccacctcagg cgacgacctc gagatcgatt tcgatcttcc gacgtcggag
     3661 caggacaacg tgtccgttgg cgatgcagtc gaagtga
//
LOCUS       DATITW010000198         1959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_49917, whole genome shotgun sequence.
ACCESSION   DATITW010000198 DATITW010000000
VERSION     DATITW010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49917"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..>1959)
                     /locus_tag="VLS86_02760"
     CDS             complement(169..>1959)
                     /locus_tag="VLS86_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HSO49443.1"
                     /translation="TIIAVDEDVIGLFLGRPDLQFWRGAFYIGYSLVVDVFSLGFIAG
                     LLFLAWRRRQRPSRLNYARVDREQDSSDRRGYTLDDRAFLIILLFLGLSGLLLEGFRI
                     VATEFPDFELASVGGWLVALLTSGLGPEGNETARIVTWWIHAVTALAFIASIPYSKAM
                     HMVVDVANLALTDPDSARRLPAADGQAAAVQLKAFTVKELVDFDSCTKCGRCHEVCPA
                     RASGAPLSPRDLILDLREWADGLSRINLWYGPGAQPRHQNTVGLAGGVIAAETLWACT
                     TCMACMEVCPVGIEHVPTIVGLRRQLVDKGEMEPGLQNAFQALTRAGNSFGQPAKQRA
                     RWTKGAQVEVKDARVQPVEWLWFVGDYASYDPRAQQVSQTVARLLERAGIDFGILFDA
                     ERNSGNDVRRAGEEGLFDMLRDQNREALAAASYRRIFTTDPHTLNALCHEYGIPEALH
                     YTQLLDGLVASGELVLGRLDGKATYHDPCYLGRYAEEYEAPRTLIKATGLDLIEMGRC
                     RENSFCCGAGGGRIWMDDSGLAERPSENRIREAVALGDDVRYFVVACPKDLVMYSEAV
                     RSTGHESRLEVVAVAQRLERALEMEAVPIG"
BASE COUNT          356 a          645 c          641 g          317 t
ORIGIN      
        1 gactcgagtc gatcttcgcg gtcgatcacc ccgatcaggt aggagagcaa ggcgaacgcg
       61 gcctgcccga gggacgaggc caacttgccg gagggctccg ccacagcctg ctcggccaaa
      121 gcctgctcca atcgtgggga ccgggagatc gacaccggga tcgccttctc agccaatcgg
      181 gaccgcctcc atctctagcg cccgctccag tcgctgggcg acggcgacca cctccagcct
      241 gctctcgtgg ccggtgctcc tcaccgcctc cgaatacatc accaagtcct tggggcaggc
      301 gaccacgaaa taacggacat cgtcgcccag ggccaccgct tcccgtatcc ggttctcgga
      361 gggacgctcg gccagccctg agtcgtccat ccagatgcgt ccgccgccgg cgccgcagca
      421 gaagctgttt tctcggcacc gtcccatctc gatcagatcg agaccggtgg ctttgatcaa
      481 tgtacggggc gcctcgtact cttcggcgta acggcccagg tagcaggggt cgtggtaggt
      541 ggccttcccg tccagcctgc ccaacaccag ctcacccgac gcgaccaggc cgtcaagcag
      601 ctgcgtgtag tgcaatgcct ccggtattcc gtactcgtgg cagagggcgt tgagggtgtg
      661 cgggtcggtg gtgaagattc gccgatacga ggcggcggcc agcgcctccc ggttttgatc
      721 gcgcagcatg tcgaagagcc cctcctcgcc tgcgcggcgg acgtcgtttc ccgagttgcg
      781 ctcggcatca aagagtatcc caaagtcgat cccggcccgc tcgagcagtc gagcgacggt
      841 ctggctgacc tgctgggccc gggggtcgta ggaggcgtag tcgccgacga accacagcca
      901 ttccaccggt tggacccggg cgtccttgac ttcaacctgg gcgcccttgg tccagcgggc
      961 gcgctgtttg gcgggctgtc cgaagctgtt gccggcccgg gtcagcgcct ggaaggcgtt
     1021 ctgcaagccc ggctccatct ctcccttgtc cactagctgg cgacggagcc cgacgatggt
     1081 ggggacgtgt tcgatcccca ccggacagac ctccatgcac gccatgcagg tggtgcaggc
     1141 ccacagggtc tcggcggcga tcacaccgcc ggccagcccg accgtattct gatgacgggg
     1201 ttgggcgccc gggccgtacc acagattgat ccgggagagg ccatcggccc actcccgtag
     1261 gtcgaggatg aggtcacgag gcgagagggg tgccccggag gcgcgggcag ggcaaacctc
     1321 gtggcagcga ccgcatttgg tgcaagagtc gaaatcgacc aactccttca ccgtgaacgc
     1381 tttcagttgc actgccgcgg cctggccgtc ggcagccggg agacgtcggg ccgagtccgg
     1441 gtcggtgagg gccaggttgg cgacatcgac caccatgtgc atcgccttcg agtaggggat
     1501 agaggcgatg aacgccagag cggtaacggc gtgtatccac catgtcacaa tccgcgccgt
     1561 ctcatttccc tcgggtccca acccggaggt gagcagagcc accagccatc cccccaccga
     1621 agccagttcg aaatcgggaa actcggtggc gacgatgcgg aatccctcca gcagcaggcc
     1681 gctcagtccc aggaaaagga ggatgatgag gaaggcgcgg tcgtcgagcg tgtagccgcg
     1741 ccgatcggaa gagtcctgct ctcgatcgac tcgggcatag ttcaggcggg aggggcgctg
     1801 gcgtcgccgc caggccagga acagcagccc ggcgatgaaa cccaacgaga agacatccac
     1861 caccagggag tagccgatgt agaaggcccc tctccagaac tggagatcgg gccgaccgag
     1921 gaagagcccg atcacgtcct cgtcgacggc gataatggt
//
LOCUS       DATITW010000199         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_129782, whole genome shotgun sequence.
ACCESSION   DATITW010000199 DATITW010000000
VERSION     DATITW010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129782"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VLS86_02765"
     CDS             <1..309
                     /locus_tag="VLS86_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HSO49444.1"
                     /translation="HLSYLGDASIGAGSNIGAGSITCNYDGYQKHRTEIGAEVFIGSD
                     TMLVAPLTIGDRAVTGAGSVITRDISGGALAVERGEQREIPGYSDRRAERHAAKEAGD
                     "
     gene            335..1282
                     /locus_tag="VLS86_02770"
     CDS             335..1282
                     /locus_tag="VLS86_02770"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HSO49445.1"
                     /translation="MMLFTGGANITLAEEVAEVLGVALGDLERSRFANGEIYVRPAES
                     VRGADCYVMQSHCEPMNDNIMEHLITIDALKRASARRITAVMPFFGYSRQDKKVRPRE
                     PITARLVGDLFMTAGADRLVSVDLHTGQLQGFIHKPFDSLTALPIITDYLAERLDGPT
                     TVLSPDAGGVKRAERYARFLDASVAFVYKRRDPTQHNVSSALEMAGTVKDRHVVIVDD
                     IIDTAGTVTNAAEMVRAQGAATVRIAATHGVFSEPAVDRIKNAPVEEVIVTNTLPVSP
                     AVLELDNVRVLSIAPILGETLQAIFMDSSVSEIFLGENA"
     gene            1325..2653
                     /locus_tag="VLS86_02775"
     CDS             1325..2653
                     /locus_tag="VLS86_02775"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSO49446.1"
                     /translation="MSARHRQSDSPTQARVRALIIGLVVVFLIAFFFLRGSGESGPGE
                     DPPSTGATGTTVPPDSAGTTVPGAVTTTPGNGSSTTGSVTPLQAVRLETVAQDFRQPT
                     VLTAPVGDDRLFIAQRVGVIRILDADRQMLEPAFLDISDRVLANGIEQGLLGLAFHPD
                     YASNGRFFVYYTDREGRRQLSEFQVSVSDPNLVSPDTERVLFEFEQPPDATDIRHYAG
                     QLIFGPDGYLWVSLGDGADSRDQGQDPNTLFGTVSRIDVDNGDPYGIPPENPFVAGGG
                     APEVWAYGLRNPWRFSIDPVERLIYIADVGHADQEEINVLPIDEGGFNLGWSDVEGTR
                     CFHVQDCDLANYTAPVLTYSHADGCSVTGGFVYRGTEIPELVGTYFYSDWCSQWIKSF
                     RYVDGQVTEEIDWSDDLGQIGQINSFGLGGDGELYAVTHDGVVARFVADR"
     gene            complement(2650..>2878)
                     /locus_tag="VLS86_02780"
     CDS             complement(2650..>2878)
                     /locus_tag="VLS86_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HSO49447.1"
                     /translation="EEVVGPTADSVVVEPGDHLWKISQSHVEQVLSRAAEPDEVEPLW
                     RSVIDANRQRLRSGDPDLIYPGETILIPVAG"
BASE COUNT          494 a          957 c          921 g          506 t
ORIGIN      
        1 cacctctcct atctcggtga cgccagcatc ggcgccggct ccaacatcgg cgccgggtcg
       61 atcacctgca actacgacgg ataccaaaag catcgaaccg agatcggtgc cgaggtgttc
      121 atcggatcgg acaccatgct ggtggcaccc ctgaccatcg gcgaccgggc cgtgaccggg
      181 gccggctccg tcatcacccg ggacatctcc ggaggggcgc ttgccgtgga gcgaggcgag
      241 cagcgcgaaa tccccggcta ctctgaccga cgcgccgaga gacacgccgc caaggaagca
      301 ggggactgag ttggacattg tttcccgcaa ccgaatgatg ctcttcaccg ggggcgcaaa
      361 catcaccctc gccgaggaag tggccgaggt gctgggtgtc gcattgggtg atctggagcg
      421 gtcccggttt gccaacggcg agatctatgt ccgtcccgcc gagagcgttc gtggcgccga
      481 ctgctacgtg atgcagagcc attgcgagcc catgaacgac aacatcatgg agcacctgat
      541 cacgatcgat gccctgaagc gggcgtccgc tcgtcgcatc accgccgtca tgccgttctt
      601 cggctattcg agacaggaca agaaggtgcg gccccgggag ccgatcacgg cccggctggt
      661 gggcgacctg ttcatgaccg caggcgcgga tcgattggtg tcggtcgatc tccacaccgg
      721 gcagttgcag ggtttcatcc acaagccctt cgactcgctg accgctctgc cgatcatcac
      781 cgactacctc gccgagcgcc tggatggccc gaccacggtc ctgtcacctg acgccggcgg
      841 tgtgaagcgg gcggaacggt acgcccgctt cctcgatgcc tcggtcgcct tcgtctacaa
      901 gcggcgcgac ccgacacagc acaatgtgtc ctcggctttg gagatggcag gaacggtcaa
      961 ggacaggcac gtggtgatcg tcgacgacat catcgacacc gcgggcacgg tcaccaacgc
     1021 cgcggagatg gtccgggccc agggtgccgc caccgtgcgc atcgccgcca cccacggtgt
     1081 cttctccgag ccggcggtgg atcggatcaa gaatgcgccg gtggaagagg tgatcgtcac
     1141 caacaccctg ccggtctcac cggcggttct cgagttggac aatgtccggg tgctgtccat
     1201 cgccccgatc ctcggcgaga cgctgcaggc gatcttcatg gactcgtcgg tttcggagat
     1261 cttcctcggc gagaacgcat agccccgccc gaaccggcgt ggcggttcct acacttgccc
     1321 cttcgtgagt gcgcgccacc gccaatctga ttccccgact caagcccggg ttcgcgctct
     1381 catcatcggg ctggtggtcg tcttcctgat cgccttcttc ttcctgcgcg gctcaggcga
     1441 aagcggtcca ggcgaggacc ccccttcgac cggggcgacc ggcaccaccg ttccacccga
     1501 ttcggcgggc accaccgtcc ccggcgccgt gaccaccacc ccgggcaacg ggtcgagcac
     1561 caccggcagc gtgactcctc tccaggcggt gcggctggag actgtggccc aggacttccg
     1621 tcagccgacg gtcctcacgg caccggtcgg cgacgaccgt ctgttcatcg cccagagggt
     1681 tggcgtgatc aggatcctcg acgccgatcg ccagatgctg gagccggcgt tcctcgacat
     1741 ctcggatcgg gtcctcgcca atgggatcga gcagggtctt ctcgggctcg ccttccatcc
     1801 cgactacgcc agcaacgggc gcttcttcgt ctactacacc gatcgggagg ggcgtcgtca
     1861 gctctcggag ttccaggtct cggtgtcgga cccgaatctc gtctcgccgg acacggagag
     1921 agtgttgttc gagttcgagc agccgccgga cgccacagat atccggcact acgcgggtca
     1981 gctgatcttc ggcccggacg gatacctgtg ggtgtcgttg ggagatggtg ccgactcgag
     2041 ggaccagggc caggacccga acaccttgtt cggcaccgtc tctcgaatcg acgtcgacaa
     2101 cggcgacccg tacggcatcc cgcccgagaa cccgttcgtt gccggcggtg gcgcgccgga
     2161 ggtgtgggcc tacgggctga ggaacccatg gcgattctcg atcgaccccg tcgagcggtt
     2221 gatctacatc gccgacgtcg gccacgccga ccaggaggag ataaacgtgc tccccatcga
     2281 tgagggaggt ttcaacctgg gctggtcgga cgtggagggc acccggtgct tccatgtcca
     2341 ggactgtgac ctggccaact acaccgcccc ggtgctgacc tactcccacg cggacggatg
     2401 ctcggtcacc ggggggttcg tctatcgagg caccgagatc cccgagctgg tggggaccta
     2461 cttctacagc gactggtgct cgcagtggat caagtcgttc agatacgtcg acggccaggt
     2521 gaccgaggag atcgattggt cagacgatct cgggcagatc gggcagatca acagctttgg
     2581 cctcggggga gacggtgagc tgtacgcagt gacccacgac ggcgtggtcg ccaggttcgt
     2641 ggccgatcgc tagcccgcga ccgggatcag gatcgtctcc ccggggtaga tgaggtcggg
     2701 gtcaccggat cggagacgtt gccggttggc gtcgatcacc gaacgccaca acggctcgac
     2761 ctcgtcgggc tcggcggctc gggacagcac ctgttccaca tggctctggc tgatcttcca
     2821 caggtgatcg ccaggttcca ccaccaccga atcggcagtc gggcccacga cttcctcg
//
LOCUS       DATITW010000200         1831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_9874, whole genome shotgun sequence.
ACCESSION   DATITW010000200 DATITW010000000
VERSION     DATITW010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9874"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VLS86_02785"
     CDS             <1..552
                     /locus_tag="VLS86_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019871380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSO49448.1"
                     /translation="RIEVQGHQVTVVDLHNLNDRAKRFVVGVTVRGAFEDKERAGQSD
                     ELLFLVLDELNKYAPREGFSPIKEVLLDVAERGRSLGVILIGAQQTASEVERRIIANS
                     SIRVVGRLDSAEASREEYGFLPSAQRQRATILKPGTMLLAQPELPVPLVLEFPFPAWA
                     TRASEAGAMPPADNDPVDPFHDL"
     gene            618..1748
                     /locus_tag="VLS86_02790"
     CDS             618..1748
                     /locus_tag="VLS86_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HSO49449.1"
                     /translation="MRLLHTSDWHVGKKIRGRSRAAEHRDVLTEIVAIADSRRVDVAL
                     VAGDLFDVSSPSPEDEAIVYRALLDLAEICPVLVVAGNHDNPGRLEAVKPLLDLGRIT
                     VVARPAQPADGGVTVLDTLGLKVALLPFVSQRGIVKAEQIMDLDADQHAQSYEARLRR
                     IIDTLTEGMDLDTVNVVAGHLTVYGAAPGGGEREAHIFGYAIPPQAFPGSLSYVALGH
                     LHRQQRIAASAPVWYSGSPLQLDFGETGDEKGVLVVDVEAGKPARVEPVPLASGVRLV
                     QLSGSMEQVLAMASEVEGAYVKILLDEQARAGINEEVRAAIPGAIDVVIARPDEARSR
                     ERATRAGRSPVELFETYLSSIGVEDPAVVDLFRELEQEVAST"
BASE COUNT          298 a          606 c          613 g          314 t
ORIGIN      
        1 cggatcgagg tccagggtca ccaggtgaca gtggtcgatc tgcacaatct caacgacagg
       61 gccaagcggt tcgtggtcgg ggtgacggtc agaggcgcct ttgaggacaa ggagcgggcg
      121 gggcagtccg atgagttgct gttcctggtg ctcgacgagc tgaacaagta cgccccacgc
      181 gagggattca gcccgatcaa ggaagtcctc ctcgatgtcg ccgagcgggg ccgctccctg
      241 ggtgtgatcc tgatcggggc ccagcagaca gcttccgagg tggagcgtcg catcatcgcc
      301 aactcgtcga tccgggtggt gggtcggctc gactctgcgg aggcctcacg cgaggagtac
      361 ggcttcctcc cgtcggccca gagacaacgg gccacgatcc tcaagccggg gaccatgttg
      421 ctcgcccaac cggagctgcc ggtccctctg gtgctcgagt tcccgttccc cgcctgggcg
      481 acgagggcct ccgaggccgg agccatgccc ccggccgaca acgacccggt ggatcccttt
      541 cacgacctgt agcgacttcc ccgattctgt ccctgcccgg ttctacgatc gggggcaatg
      601 aggctcgagg caaccagatg aggctgctcc acacctccga ttggcacgtc gggaagaaga
      661 tccgcggtcg ctcccgagcc gccgagcatc gtgacgtcct gaccgagatc gtcgccatcg
      721 ccgacagccg gagggtcgat gtcgctctcg tcgccggcga cctgttcgac gtctcctctc
      781 cttcaccgga agacgaggca atcgtctatc gggcgctgct cgacctcgcc gagatctgcc
      841 cggtcctggt ggtcgccggt aaccatgaca acccgggcag gctggaggcg gtcaagccac
      901 ttctcgatct tggtcggatc accgtggtcg cccgtcctgc ccagccggcg gacggcgggg
      961 tcacggtcct cgacacgctc ggtctcaagg tcgccctcct ccctttcgtc tcccagcgag
     1021 ggatcgtcaa ggcagagcag atcatggacc tggacgccga tcaacatgct cagagctacg
     1081 aggcccggct gaggcggatc atcgacacgc tgaccgaggg catggacctc gacacggtga
     1141 acgtcgtggc cgggcatctg acggtctatg gcgcggcgcc cggcggaggg gaacgagagg
     1201 cgcacatctt cggctacgcc ataccccccc aggccttccc cggcagtctc tcctatgtgg
     1261 cgcttggcca tcttcatcgc cagcagcgga tcgcggcctc ggctccggtc tggtacagcg
     1321 gctcgccact ccagctcgat ttcggtgaga caggggacga gaagggagtg ctcgtcgtcg
     1381 acgtcgaggc gggcaagccg gctcgggtcg agccggttcc ccttgcctcc ggggtgaggc
     1441 tggtccaact tagcggcagc atggagcagg tgctggcgat ggcctccgag gtcgaggggg
     1501 cctacgtgaa gatcctgctc gacgagcagg cccgcgccgg gatcaacgag gaggtacgtg
     1561 ccgccatccc cggggcgatc gatgtcgtca tcgcccggcc cgacgaggcg aggtcacggg
     1621 agcgggcgac gcgggccggt cggtcgcctg tcgagctgtt cgagacctac ctctcatcga
     1681 ttggtgtgga agaccccgcg gtcgtcgacc tgtttcgcga gctggaacag gaggtggcat
     1741 cgacgtgaga ccccggcagc tcatcatgga gggctttctc gcctaccgcc accgcaccga
     1801 ggtcgacttc accgacgccg acctcttcgt g
//
LOCUS       DATITW010000201         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_89974, whole genome shotgun sequence.
ACCESSION   DATITW010000201 DATITW010000000
VERSION     DATITW010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89974"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VLS86_02795"
     CDS             complement(<1..846)
                     /locus_tag="VLS86_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSO49450.1"
                     /translation="MKSVGVARPWCGRLGKVDNCQVGVSMAYVSRKEHGIVDTRLYLP
                     AEWANDRGRREEAGVPESITFQTRHGLALEMLKEGGGRLPHAWVAGDDEMGRPSRFRQ
                     ELRTANERYLLGVPSNLLIRDLEVTPPEDSGRGPHPQVPFARLDRWCRALPEVAWPRI
                     DVRDGEKGPLVIEAVKRRIQARTETGGTGPEELLFVTRGRQANGTSKHDYDFSNADPG
                     TPREERARVAKAEHRVEECFERAQGEAGLADSQGRNWMAWHHHQILALLAAWFLTQET
                     RRGKNP"
     gene            complement(843..1223)
                     /locus_tag="VLS86_02800"
     CDS             complement(843..1223)
                     /locus_tag="VLS86_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSO49451.1"
                     /translation="MERRYEARLEEMWEDAEVPPAIVEGMLTRLETVVEPFAEALDAP
                     AQRRHALEYMTGLLSDLKRKTGEGIAYLQDQKRQGIPKFIGHVPWDHQPLFATLADQV
                     GEQLGEPDAVLVFDPSGFAKKGRE"
     gene            1360..>2100
                     /locus_tag="VLS86_02805"
     CDS             1360..>2100
                     /locus_tag="VLS86_02805"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HSO49452.1"
                     /translation="MTVGSDPIFTTPANNPISILDHFADLPDPRREQGRIHRLDEIVF
                     IATCAVFCGADTWVQIADYGHSKRDWLETFLTLPGGIPSHDTFRRVFCLLDPVAFQKG
                     FSAWITALMARNGLTPIATAPHELMPIAIDGKAQRGSARRTVGQSALPVVSAWSVENH
                     LTLGQVATDAKSNEITAIPELLELLDLNGAVVTIDAMGCQKEIAAEIVGRGGQYVLAA
                     KENPPHLYEDIKRAFDKALDQGEPGVDFT"
BASE COUNT          362 a          763 c          594 g          381 t
ORIGIN      
        1 tggatttttt ccccgccgcg tctcctgggt caggaaccac gcggccagca gcgccaggat
       61 ttgatgatga tgccaggcca tccagttccg accctgagaa tcggccaacc ccgcctcacc
      121 ctgcgcccgc tcgaagcatt cctcgacgcg atgctccgcc ttcgccaccc gcgcccgttc
      181 ctcccgcggt gtgcccgggt ccgcgttcga gaaatcgtag tcatgcttgg aggtgccgtt
      241 cgcttgccgt ccccgcgtca cgaacagcaa ctcctcggga cccgtacccc ccgtctcggt
      301 gcgggcctgg atccgacgct tgaccgcttc gatcaccaac ggccctttct cgccgtcacg
      361 cacatcgatc cggggccagg cgacctccgg cagcgctctg caccagcgat ccaaccgcgc
      421 gaaggggacc tggggatgtg gtccccgccc cgagtcctcc ggcggtgtca cctcgaggtc
      481 gcggatcagc agattcgagg ggactcccag caggtaacgt tcgttggcgg tgcgtaattc
      541 ctgccggaaa cgcgaaggac ggcccatctc gtcgtccccg gcgacccagg cgtgcggcag
      601 ccgcccgcca ccctccttga gcatctccag cgccagtcca tggcgcgtct ggaacgtgat
      661 cgactcgggc acccccgcct cctcccgacg tccccggtcg ttggcccatt ccgcagggag
      721 gtagagacgt gtatcgacga tcccatgttc cttccgcgag acgtaagcca tggagacgcc
      781 cacctggcag ttgtcgacct tcccgagccg gccgcaccac ggccgcgcca cgcccacgga
      841 cttcactccc ttcccttctt ggcgaagccc gagggatcga acacgaggac cgcgtcgggt
      901 tcccccaact gctcgccgac ctggtcggcc agcgtcgcga ataagggctg gtgatcccaa
      961 ggcacatgcc cgataaactt cgggatgccc tggcgtttct gatcctggag gtatgcgatc
     1021 ccctcgccgg tcttgcgctt gaggtccgac agcagtccgg tcatgtactc gagggcgtgg
     1081 cgtcgttgcg cgggcgcgtc gagtgcctcc gcgaacggct cgacgacggt ctccaaccgc
     1141 gtcagcatcc cctccacaat cgcggggggg acttccgcat cctcccacat ctcctccaac
     1201 cgcgcctcgt atcgccgttc catgctcttc tccgttccag gccgcgtcct tgacgaaagc
     1261 aattggtgca aaagacatta taccagacgc aaggccggga tacagtagaa gtaccatcga
     1321 ccaccaggag aacccgatct cttcagggac gtccacacca tgaccgtcgg ctcagacccg
     1381 atcttcacga ctccggccaa taacccgatc tcgatcctcg accatttcgc ggacttgccc
     1441 gatccccgcc gcgaacaggg gcgaatccat cgactcgatg agatcgtctt catcgccacc
     1501 tgcgccgtct tctgcggcgc cgatacctgg gtgcagatcg ccgactacgg ccactccaag
     1561 cgcgactggc tcgagacctt cctcaccttg cccggcggca tcccctcaca cgacaccttc
     1621 cgacgtgtct tctgtctgct cgacccggtg gcctttcaga agggcttctc cgcctggatc
     1681 acagcgctga tggctcgaaa cggattgact ccgatcgcca cggctccgca cgaactgatg
     1741 ccgatcgcca tcgatggcaa ggcccaacgc ggctcagccc gtcgtaccgt cggccaatcg
     1801 gccctccctg tcgtcagcgc ctggtcggtc gagaatcatt tgaccttggg gcaagtggcc
     1861 accgacgcca aatccaacga gattaccgcc attcccgagt tgctcgaact cctcgacctg
     1921 aacggggccg tggtgaccat cgacgccatg ggctgtcaga aggagatcgc cgccgagatc
     1981 gtcggccggg gtggtcagta cgtgctggcc gccaaggaga atccgccgca tctctacgag
     2041 gacatcaagc gggcgttcga caaggcgttg gatcagggcg agcccggtgt ggacttcacc
//
LOCUS       DATITW010000202         1906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_530691, whole genome shotgun sequence.
ACCESSION   DATITW010000202 DATITW010000000
VERSION     DATITW010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530691"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VLS86_02810"
     CDS             complement(<1..306)
                     /locus_tag="VLS86_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSO49453.1"
                     /translation="MAHHPEGDTELLRRAYELARRSHEGQLRKTGDPYITHPVTVALM
                     LAEYGLDEQTLAAAFLHDTVEDTSLTIADISKEFGNEVGRLIDGVTKLDRVRYSNREQ
                     "
     gene            complement(347..865)
                     /locus_tag="VLS86_02815"
     CDS             complement(347..865)
                     /locus_tag="VLS86_02815"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HSO49454.1"
                     /translation="MERLTSLIRDIPDFPEPGVVFKDITPVLADGPAFSMLIDSLAAP
                     FHDTGVSKVAGIEARGFTLASPVAGRLHAGFIPVRKPGKLPYHTVREDYTLEYGTDSL
                     EIHTDSAVPGEKILLVDDVIATGGTAAAAIRLLRGIGADVVGFAVFIELGFLDGARML
                     DGVPLHALVTYD"
     gene            complement(887..1186)
                     /gene="yajC"
                     /locus_tag="VLS86_02820"
     CDS             complement(887..1186)
                     /gene="yajC"
                     /locus_tag="VLS86_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HSO49455.1"
                     /translation="MTHVVLAQEAAGSPLPSLIFLGLMVGVFWLFIIRPQRNRAKQQQ
                     AVAAGLTAGQQVRTIGGIHGTVVSVDEESVVIRVEEGKIRVSRRAIGSVVEPDQT"
     gene            complement(1241..>1906)
                     /locus_tag="VLS86_02825"
     CDS             complement(1241..>1906)
                     /locus_tag="VLS86_02825"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HSO49456.1"
                     /translation="LPAPRERVQAEMERTLRRAERRLSAHTRADQALFGIVQGGVDPD
                     LRAESAARTAQLGFPGFGIGGLSVGETNEERRVALEAAIAERPETKPRYVMGLGDTEG
                     LLEAIGRGADLFDCVLPTRLARHGRVLTRFGDFNLRNAEYEGDDRPIDSACPCLACRG
                     YSRAYLRHLVRMKELSALRLLTIHNLAYTNELVRNAGEAIALGGFDIFVESVTAQRSN
                     GSP"
BASE COUNT          331 a          635 c          601 g          339 t
ORIGIN      
        1 ctgttcccga ttcgagtagc ggactcgatc gagcttggtc acaccgtcta tcagccggcc
       61 cacctcgttg ccgaactcct tcgagatgtc ggcgatggtg agcgatgtgt cctcgacggt
      121 gtcgtggagg aaagccgcag ccagtgtctg ctcgtccagc ccatactcgg ccagcatcag
      181 ggccacggtc acgggatggg tgatgtaggg gtcaccggtc tttcgcaact ggccttcgtg
      241 ggaccgtctc gccagctcgt aggcgcgtct cagcagctcg gtgtcgccct ccgggtggtg
      301 ggccaggaag gcagcgacaa cgtcttcgaa ttctgcctgt tgggtttcag tcgtaggtga
      361 caagggcatg taggggcacc ccatcgagca tccgcgcgcc gtcgagaaag ccgagctcga
      421 tgaagacagc gaacccgacc acgtcggcac cgatcccccg taacaaccgg atcgcggcgg
      481 cagctgttcc cccggtggcg atcacgtcgt cgaccagcag gatcttctcc ccaggaacgg
      541 ccgagtcggt gtggatctcc agggagtcgg tcccgtactc gagggtgtag tcctcccgga
      601 ccgtgtgata gggcagcttt cccggtttgc gaacgggtat gaaaccggcg tgaagacgac
      661 cggccaccgg gctggcgagg gtgaacccac gggcctcgat ccctgccacc ttggacacgc
      721 ctgtgtcgtg aaacggggcc gccagggagt cgatcagcat ggagaacgcc gggccgtccg
      781 ccagaaccgg tgtgatgtcc ttgaacacca ccccgggctc cggaaagtcg ggaatgtcgc
      841 gaatcaagga ggtgaggcgt tccacgacct gaagtctacg gtcggatcat gtctggtccg
      901 gttcgactac cgagccgatt gcccgccgtg acacccggat cttcccctct tcgaccctga
      961 tgacgacact ctcctcgtcg actgacacca ccgttccgtg gatccccccg atggtccgaa
     1021 cctgctgtcc ggcggtcagc ccggcggcca cggcctgctg ctgcttggcg cggtttcgct
     1081 gcggcctgat gatgaacaac cagaaaacgc caaccatcaa cccgagaaag atcagcgagg
     1141 gaagcgggct tcccgcggcc tcctgagcca ggacaacatg ggtcacaggg tcctccagtt
     1201 gatgagttcg gacggcggga ggttaggcgc cggccaccgt ctacggcgaa ccgttcgaac
     1261 gttgggcagt caccgattcc acgaagatat cgaagccacc gagggcgatc gcttcgcctg
     1321 cattgcgcac caactcgttg gtgtaggcca gattgtgaat cgtgagcagt cggagggccg
     1381 acagctcctt catcctcacc aggtgccgaa gataggcgcg ggagtaaccc cggcaggcca
     1441 ggcagggaca agcgctgtct atggggcggt cgtcgccctc gtactcggcg ttgcgcaggt
     1501 tgaaatcgcc aaagcgggtc agcaccctgc catgtcgcgc cagccgggtg ggtaggacgc
     1561 agtcgaacag gtcggcgccc ctcccgatcg cctccagcag cccctcggtg tcccccaggc
     1621 ccatgacata tcgcggcttg gtctcgggcc gttccgcgat cgccgcctcg agcgcgactc
     1681 ttctctcctc gttcgtctcc cccaccgaaa gccccccgat cccgaatccg ggaaagccga
     1741 gctgcgccgt cctcgccgcg ctctccgcac gaaggtcggg gtcaaccccg ccctggacga
     1801 tcccgaacag ggcctgatcg gctctggtgt gtgccgaaag tctccgttcg gcccttcgca
     1861 gcgtccgctc catctcggcc tgcacccgct ctcggggcgc gggcag
//
LOCUS       DATITW010000203         3435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_129901, whole genome shotgun sequence.
ACCESSION   DATITW010000203 DATITW010000000
VERSION     DATITW010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129901"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VLS86_02830"
     CDS             complement(<1..1003)
                     /locus_tag="VLS86_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSO49457.1"
                     /translation="MSDLIVHLAPAVVPVTGPPLTQGAVAVANGRIVAVGAREEILGI
                     SVDAATIEWEGVLIPGLVNAHTHLQYTSFAEVGALTHPSYVAWSERFVTEYEARRSDD
                     WGAVARAGVELALATGTTCFADVVTDLAALDALADMEVSGVSYLELIGVDEQAWEAGV
                     RDRVMATLRGAHRSPHAGVGLSPHAPYSVDEPVLKKAATLARVEGVRLHIHLAESDSE
                     DSYYRTGTGALAERVTLRVGRPWSILARGGTGLGAAEFAAACGLLGSDSHVAHGVYLG
                     SEGRAILRRTGTYVALCPRSNLTVGIEPPPVADFLAEESPIAVGTDSLGSNTSLDLLE
                     "
     gene            complement(1000..2202)
                     /locus_tag="VLS86_02835"
     CDS             complement(1000..2202)
                     /locus_tag="VLS86_02835"
                     /inference="COORDINATES: protein motif:HMM:NF004005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HSO49458.1"
                     /translation="MAETGRGMIEFARQFMPALRAATGIVAENGRLAGRRVGIALTLE
                     PKTACLVEAVASLGADVAVFGSGHSTKGDVVAALAESGVKVFAEAGAALDRVAELRDR
                     FIDTRPEYLCDDGAGIVRRMHGDRRDVLDELRGVAEETTSGVRPLRVMESEGALMVPC
                     VAVNDARTKVLFDNVYGTGQSVVMAVLDATNLQMSGKTVVVVGYGMVGRGIASVARGL
                     GARVTVTEIEPVEALVAMHDGMRVCPLSEAVADADFIFTATGIGHSLTAHHVEDMKDG
                     AVIAVGGAGPPEFDPGIGPGLVAGDEVRPKVRALATASGTTVFVVADGYCANTSAGEG
                     NPIEIMDLSLALQLRALDFLAAGGLSPGIHLLPRHIEDEVALSQLEANGIQIDQPTQD
                     QIRAAGSW"
     gene            complement(2195..>3435)
                     /locus_tag="VLS86_02840"
     CDS             complement(2195..>3435)
                     /locus_tag="VLS86_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HSO49459.1"
                     /translation="PTGHRAIDWARRHSPVLDGYVRSRLDDGSLRGRRVAVMVHLEAK
                     TAFLATVLADAGAEVVVAGSNPWTTRDDVAAALVERGIEVHSSRRSSAEVWESDLMAV
                     ADTAPELIVDDGAELTVRISRDRPQVYSGLSGVSEETTTGVARLRQLERAGRLPMPAI
                     AANDAACKHMFDNRYGTGQSTIQAILQLTNLRMSGKTVVLVGYGWVGRGIATYAHNLG
                     GRVIVVEIDPIKALEAHTDGHEVSGLRAALARADLVVTATGGMRAIGKEDLDAIAPSA
                     VLANAGHHDLEIDVPALEAAAGSSEEVRPGVTRYLLGDDRPVFVLSGGALVNIAGGLG
                     HPIEIMDLSFSVQALGCHLLARGGLPAGVHDFPRDLDHAIATAKLESRGIALDLPDLA
                     QQDTLTGILEVGDRAGGEHG"
BASE COUNT          535 a         1211 c         1167 g          522 t
ORIGIN      
        1 cctcgagcag gtcgagtgag gtgttcgacc ccagtgagtc ggtgccgacc gcgatcgggc
       61 tctcctctgc caggaagtcc gccaccggag gaggctcgat tccgacggtg aggttggagc
      121 gggggcagag tgcaacatag gtgccggtcc ggcgaaggat cgcccgcccc tcgctcccca
      181 ggtacacccc gtgggccacg tggctgtcgc tcccgagcag cccgcaggca gcggcgaact
      241 ccgcggcgcc caaaccggtc ccaccccggg ccaggatgct ccaaggccga ccgactcgaa
      301 gcgtgacccg ctccgccagg gcgccggtcc cggtgcggta gtaggagtcc tcgctgtcgg
      361 actcggcgag atggatgtga aggcgcaccc cttcgacgcg ggccagggtg gcggccttct
      421 tcagcaccgg ctcgtcgacg ctgtacggcg catgcggaga gagacccacc ccggcgtggg
      481 gagagcggtg cgcaccgcgc agagttgcca tcacccgatc cctgaccccc gcctcccatg
      541 cctgctcgtc gacgccgatc aactcgaggt aggacacgcc ggagacctcc atgtcggcca
      601 gggcgtcgag ggcggcgagg tcggtgacca cgtcggcgaa gcaggtggtg ccggtggcca
      661 acgccaactc cacgccggcc ctggccacgg cgccccagtc gtccgagcgg cgggcttcgt
      721 actcggtgac gaaacgctcc gaccaggcga catagctggg atgggtcagg gcgcccacct
      781 cggcgaacga ggtgtactgg agatgggtgt gggcattgac cagacccggg atcaacaccc
      841 cctcccattc gatcgtcgcg gcatcgacgg agatccccag gatctcttct cgggcgccca
      901 cggcgacgat cctcccgttg gccacggcca ccgctccctg ggtcaggggt gggcccgtca
      961 ccggcaccac ggccggggcg aggtgcacga tcaggtcgct caccagctcc cggcggctcg
     1021 gatctggtcc tgggtcggct ggtcgatctg aatcccgttc gcctcgagct gggacagggc
     1081 gacttcgtcc tcgatgtgac ggggcagcag gtggatcccc ggggagagac ccccggcggc
     1141 gaggaagtcg agcgccctga gctgcagggc gagggacagg tccatgatct cgatggggtt
     1201 gccctcgccg gcgctggtgt tggcgcaata gccgtcggcg acgacgaaga ccgttgtgcc
     1261 cgatgcggtg gccagtgctc gcaccttggg ccgcacctcg tcgccagcca ccagcccggg
     1321 gccgatgccg ggatcgaact cgggcggccc cgcccctccg accgcgatca ccgctccgtc
     1381 cttcatgtcc tccacatggt gggcggtgag agagtggccg atcccggtgg cagtgaagat
     1441 gaagtcggcg tcggccactg cctcggagag ggggcagacc cgcatcccgt catgcatcgc
     1501 taccagggcc tcgaccggct cgatctcggt gaccgtgacc cgggccccga gccctctcgc
     1561 caccgaggcg atgcctctgc ccaccatccc gtagccgacg acgaccacgg tcttgcccga
     1621 catctgcagg ttggtggcgt cgagcacggc catcaccacc gattggccgg tgccgtagac
     1681 gttgtcgaag agcaccttgg ttcgggcgtc gttgaccgcg acacaaggga ccatgagcgc
     1741 cccttcgctc tccatcaccc gaagcgggcg gaccccactc gtcgtctcct cggccacgcc
     1801 ccgcaactcg tcgaggacat cccgtcgatc cccatgcata cggcggacga tgccggcgcc
     1861 gtcgtcgcac aggtactcgg gtctggtgtc gatgaaccgg tccctcaact ctgcgacccg
     1921 atccagggcg gctcccgcct cggcgaagac ctttacaccg ctttcggcaa gggcggccac
     1981 gacatccccc ttggtcgaat gcccgctgcc gaataccgcg acgtcggcgc cgagcgaggc
     2041 aaccgcctcc accaggcacg cagtctttgg ctccagggtg agggcgattc ccacccgacg
     2101 cccggcgagc cgcccgttct ccgcgacgat gccggtcgct gcccggagcg cgggcatgaa
     2161 ctggcgggcg aattcgatca tcccccgacc ggtttcagcc atgttcgcca ccggcccggt
     2221 caccgacctc gagtatcccg gtcagggtgt cctgctgggc caggtcgggg aggtcgaggg
     2281 cgatgccccg actctccagc ttggcggttg cgatcgcatg gtcgaggtcc cgtgggaagt
     2341 cgtggacacc ggccggcagg ccgccacggg ccaggagatg acagcccagc gcctggacgg
     2401 agaacgacag gtccatgatc tcgatggggt ggccgagacc gccggcgatg ttgaccagcg
     2461 ctccgccgga gaggacgaag acgggtctgt cgtcccccag gaggtagcgg gtgaccccgg
     2521 gtcggacctc ctcagacgag ccggccgcgg cctccagcgc gggcacgtcg atctccaggt
     2581 cgtgatgacc ggcgttggcc agcaccgcgg agggagcgat cgcatcgagg tcctctttgc
     2641 ctatcgcccg catcccgccg gtggcggtga cgacgagatc ggcccgggcc agggcggccc
     2701 tcagccctga aacctcatga ccgtcggtgt gggcctccag tgccttgatc gggtcgatct
     2761 cgacgacgat cacccgcccc cccagattgt gggcataggt ggcgatcccc cggcccaccc
     2821 atccgtatcc aacgaggacg acggtcttcc ctgacatgcg gagattggtg agctggagga
     2881 tggcctggat ggtcgattgc ccggtcccat acctgttgtc gaacatgtgc ttgcaggcgg
     2941 cgtcgttggc ggcgatggcc ggcatcggga gacgaccggc tcgctccaac tggcggaggc
     3001 gggccactcc ggtggtggtc tcctcggaga ccccactcag ccctgagtag acctgggggc
     3061 gatcgcggct gatccgcacc gtcaactcgg cgccgtcgtc gacgatcagc tcgggcgcgg
     3121 tgtccgccac cgccatcaag tcggactccc agacctcggc agacgagcgg cgactggaat
     3181 gaacctcgat gccacgctcg accagagccg ctgccacatc gtcgcgggtg gtccaagggt
     3241 ttgagccggc caccaccacc tcggctccgg cgtcggcgag gacggtggcc aggaacgcgg
     3301 tcttggcctc gagatgcacc atgactgcca cccggcgtcc ccgaagcgag ccgtcatcga
     3361 gccggctcct cacgtacccg tcgagcaccg gcgagtggcg tcgtgcccag tcgatcgccc
     3421 ggtgcccggt gggcg
//
LOCUS       DATITW010000204         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_290329, whole genome shotgun sequence.
ACCESSION   DATITW010000204 DATITW010000000
VERSION     DATITW010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290329"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1690)
                     /locus_tag="VLS86_02845"
     CDS             complement(119..1690)
                     /locus_tag="VLS86_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HSO49460.1"
                     /translation="MTVADPHLMLAFEGTEVPAWLEQRLEESPPAGVTLFREWNMSSP
                     GQVAELTTALQEMNSSPLPLLIAVDQEGGQLIGLEGSTPFSGNMALGATDDADLAEAV
                     AAAMGRELAAVGINVNYAPVADVATQADNPSLGIRSFGEDPGQVARFVGAMVAGFSTA
                     GVHSTAKHFPGKGEARVDPHYELPLLDLDRDRLDTVELPPFRAAFEAGAELLMMGHYV
                     VPSITGSRDLPISASERGINGFVRQEMGFDGLVITDALDMGALDQGPAQVVEIITMMR
                     GGTDLLLCMPDPALQDRVRTAVERGASRGLIPEAILAASRQRIERLRESTAGGMLRPE
                     LVGSEQHLALAAELAEKSVTVVRNDDGLLPLRLTPESRILALEPQPTNVTPADTTALY
                     PPSLADALRKHHQSVTGIVYPHEPERSDVDSSVTALGEHDLAVVGTVAASRGQANLVR
                     ALLDTGKPVVTVALRTPYDLGHYPGSGTHLCTFSGHQPSLVALASVLFEGSGGRGVLP
                     VSIPGLHPRGHGLPL"
     gene            complement(1687..>2213)
                     /locus_tag="VLS86_02850"
     CDS             complement(1687..>2213)
                     /locus_tag="VLS86_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014370404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSO49461.1"
                     /translation="SRLFGVMVGLIVFPLQLALVPMLKIYQSAELTGSFLGIWLAHTG
                     FGLPLAVILLYNFISQLPKDIFESAYIDGASPFTAFTRLVLPLSVPALASFAILQFLW
                     VWNDFLVALVFLGTSSNVAVLTIELASIVGSRGEAFHLLTAAAFVSMLIPLIVFLALQ
                     RYFVRGLLAGSVKG"
BASE COUNT          411 a          699 c          774 g          329 t
ORIGIN      
        1 gccccgttga cctggatgtc gacgaatccg ggggcaagga tcgagtcgtc gctcgagctt
       61 gggtccggga gcccggtgat cagaccgtcg gagcaggtca tacccccggg caatgcggtc
      121 atagcggcag cccgtggccc ctggggtgaa gcccggggat gctcaccggc agcacccccc
      181 ggccgccact gccctcgaac aggacgctgg ccagtgccac cagtgagggc tggtgaccgc
      241 tgaaggtgca gagatgggtg ccggaccccg ggtaatggcc caggtcgtac ggagtgcgca
      301 gcgcgaccgt gaccacgggc ttgccggtgt cgagcagggc ccggaccaga ttcgcctgtc
      361 ctcgactcgc agccaccgtc cccacgaccg cgaggtcgtg ctctcccagg gcagtcaccg
      421 atgagtcgac atcgcttctc tccggctcgt gggggtacac gatcccggtg accgactggt
      481 gatgtttccg caaggcgtcg gccagggacg gtgggtagag ggcagtggtg tccgctgggg
      541 tgacgttggt cggctggggc tccagggcga ggatgcggga ctcgggtgtc agccggaggg
      601 ggagcaggcc atcgtcgttg cgcacaaccg tcaccgactt ctcggccagc tcggcggcga
      661 gagcgagatg ctgctcggag ccaaccagct ccgggcggag catgcccccg gcggtcgatt
      721 ctcggagccg ctcgatccgt tgccgggagg cggccaatat cgcctcgggg atcaggccgc
      781 gtgacgcgcc acgttccacc gcggttcgca cccgatcctg aagcgccggg tcgggcatgc
      841 agaggagaag gtcggtccct ccccgcatca tggtgatgat ctcgaccacc tgagccggac
      901 cctggtcgag ggcgcccatg tccagggcgt cggttatcac caacccatcg aatcccatct
      961 cctggcgcac gaagccgttg atgccccgct ccgaggcgga gatcggcaga tcccggctcc
     1021 cggttatcga cgggacgacg tagtggccca tcatcagcag ctcggcccca gcttcgaacg
     1081 cggcccggaa cggcgggagc tccacagtgt caagacggtc ccggtccaga tcgaggagag
     1141 gcaactcgta gtgggggtcg acacgggcct cacccttgcc cgggaagtgc ttggcggtgc
     1201 tgtggactcc ggccgtcgag aacccggcaa ccatggcccc gacgaagcgc gccacctggc
     1261 cggggtcttc tccaaagctg cgaatcccga gtgacgggtt gtccgcctgg gtggcgacat
     1321 cggctaccgg ggcgtagttg acgttgatcc ccaccgccgc cagctcgcgg cccatggcgg
     1381 cagcgaccgc ctctgccagg tcggcgtcgt cggtggcccc cagcgccatg ttgccggaga
     1441 acggggtcga tccctcgagc ccgatcagtt gaccaccctc ctggtccacg gcgatgagca
     1501 gggggagcgg gctcgagttc atctcctgaa gggcggtggt cagctcggcc acctgccccg
     1561 gcgaagacat gttccattcc cggaacaggg tcactcctgc cggtggtgat tcctcgagac
     1621 gttgctccag ccaggcaggg acctcggtcc cctcgaaggc gagcatgaga tggggatcag
     1681 cgacggtcat cctttgaccg agccggcgag gagaccccgg acgaagtatc gctggagagc
     1741 gagaaacacg atcagaggga tcaacatcga gacgaaggcg gcggcggtga gcaggtggaa
     1801 cgcctcgccg cgggagccga cgatcgaggc caactcgatg gtgaggacag ccacattcga
     1861 cgatgtgccc aagaacacca gggcgaccag gaagtcgttc cacacccaga ggaactggag
     1921 gatggcaaac gaggccaggg cggggacgga gaggggaagc accaggcggg tgaaggcggt
     1981 gaacggggaa gcgccgtcga tgtatgccga ctcgaagatg tccttgggca actgcgatat
     2041 gaagttgtag agaaggatga cagcgagagg aagcccgaat ccggtgtgtg ccagccagat
     2101 gccgaggaag ctcccggtga gctcggccga ctgatagatc ttcagcatcg gcaccagggc
     2161 caattgcagc ggaaacacga tcaggcccac catcacacca aacaaccggc tcc
//
LOCUS       DATITW010000205         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_370381, whole genome shotgun sequence.
ACCESSION   DATITW010000205 DATITW010000000
VERSION     DATITW010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370381"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VLS86_02855"
     CDS             complement(<1..1053)
                     /locus_tag="VLS86_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSO49462.1"
                     /translation="MSQTEPAPVPGRVKTAYRARLRSWGEISWDLLRLLALLAVVIWL
                     LYQVKLVVIPLMLGAFVASLGTPVVGWLDAKGLPRLLATWVVVLTTVLVAGLVGWFLV
                     AEIQGAAEEMQTALSQSWTQLEEWLDSAPFGFQGQSLSEAIERFFADSGTGAGVGGSL
                     LAGFETATEAVTGMFLTIIVSFFLIKDGERFWDWVLTKVPPDHRENTDAAGITAWATL
                     RRYLSGTAIVGIVNATVVAIALLILDVPLVMPLAILMFLGAFFPLVGAIISGAVITLT
                     VLATNGVGDALIIAGVVIVVQQLEGDLVAPLVLGRAVALHPLVILFGITAGFVIGGIV
                     GAFLTVPLIAIGAEVTR"
     gene            1304..2338
                     /locus_tag="VLS86_02860"
     CDS             1304..2338
                     /locus_tag="VLS86_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HSO49463.1"
                     /translation="MRAAIYHRFGGPISVEELPDPEVPADGVVVRVGASGLCRSDFHG
                     WDGHDRSIRLPHVPGHELAGTIEGIGPEVAGFRVGDRVTVPFAVGCGRCEQCTSGNPQ
                     ICDDDFQPGFTAWGSFAELLAIPRADLNLVPLPPLMELSSAALLGCRFATAYRAIAQR
                     GAVRPGHWVAVHGCGGLGLSAVMIAVALGARVVAVDIREQALARARDLGAELTVDGAS
                     SEDPAAVIREVTHGGAHLSLDSLGSPATATNSILSLRKQGRHVQVGLLSGGPTPLPMD
                     AVIARELEILGSHGLAAGDYAELLALVESGRVDPGLLVDREISLDQVPEELIAMRTFS
                     GDGVAVVTRF"
BASE COUNT          441 a          829 c          839 g          397 t
ORIGIN      
        1 cctggtcacc tcggcgccga tggcgatgag cggaaccgtc aggaacgctc cgacgattcc
       61 cccgatcacg aagccggccg tgattccgaa caggatcacc agcggatgga gggccacggc
      121 ccggcccagg accagtgggg caacgaggtc cccttccagt tgctgaacca cgatcaccac
      181 tccggcgatg atcagggcgt cgccaacacc gttggtggca aggacggtga gagtgatcac
      241 cgcacctgag atgatcgccc cgaccagggg gaagaaggcc ccgaggaaca tcaagatggc
      301 aagtggcatc accagtggca catccaggat cagcaaggcg attgccacca cggtcgcgtt
      361 gacgatcccc acgatggcgg tgcccgacag ataccggcgc agggtggccc atgccgtgat
      421 cccggcggca tcggtgttct cacgatgatc cggcggcacc ttggtcagca cccaatccca
      481 gaagcgctct ccgtccttga tcaggaagaa ggagacgatg atggtcagga acattcccgt
      541 gaccgcctcg gtggcggtct cgaacccggc gagcaggctg ccgccgacgc ccgctccggt
      601 cccggagtcg gcgaagaacc gctcgatggc ctccgacagc gactgccctt ggaagccgaa
      661 aggcgcgctg tccagccact cctcgagttg agtccaggac tgggagagcg cggtctgcat
      721 ctcctccgcc gcgccctgga tctcggcgac cagaaaccac ccgaccagtc cggcgacgag
      781 cacggtggtc aggacgacga cccacgtcgc cagcagtcgg ggcagaccct tggcgtccaa
      841 ccatcccacg accggtgtcc ccagactggc gacgaaggct cccaacatca acgggatcac
      901 caccaacttg acctggtaca gcagccagat cacgacggcg agaagggcca ggagacggag
      961 caggtcccat gagatctcac cccacgatcg gagacgggcg cggtacgccg tcttcacccg
     1021 gccggggacc ggtgctggct cggtctgcga catcaggagg ccattaaacc ctgctccggg
     1081 ggtgcttcat gcgtggggag gggcaggggc cgccaaaaaa agggagaagc cccatcgacg
     1141 tttccgccta cggggcttcc ccgagcttcc cacgagtgag acgctcgccg gtcttgtgat
     1201 ccagtcgttt ccgacctacg tgtccgacca gcaccatcga gaatacaccg gcgaatggcg
     1261 tctgtcaccc cacgacctcg ggtcgcaatg cgacactgag ccgatgcgcg ccgccatcta
     1321 tcaccgtttt ggcgggccga tttctgtcga ggagcttccg gaccccgaag tcccggcgga
     1381 cggggtcgtg gtccgggtcg gggcctccgg tctgtgccgg agcgacttcc atggatggga
     1441 cggccatgac cgctcgattc gcctgcccca cgtgccgggt cacgaactgg cggggacgat
     1501 cgaggggatc gggcccgagg tcgccgggtt ccgggtcgga gatcgggtga cagtcccgtt
     1561 tgcggtggga tgtgggagat gtgagcagtg cacctcgggc aacccacaga tctgtgacga
     1621 cgatttccaa cccgggttca ccgcatgggg ctcctttgcc gagttgttgg cgatcccaag
     1681 agcagacctg aatctcgtcc cgctcccgcc cttgatggag ctctcgtcgg cggcgcttct
     1741 cggctgcagg ttcgccaccg cgtaccgggc catcgcccaa cggggcgcgg tccgccccgg
     1801 gcactgggtc gcggtccatg gctgtggtgg gctgggtctg tcggcagtga tgatcgcggt
     1861 cgccctcggg gcccgggtgg tggcggtgga catccgggag caggccctgg cccgcgcccg
     1921 cgacctgggg gccgagttga cggtcgacgg cgcatcgagt gaagaccctg cggctgtgat
     1981 cagggaggtg acccacggcg gagcccacct ctcgctcgac agcctgggca gcccggccac
     2041 ggccaccaac tcgatcctct ccctgcgaaa gcagggacgt catgtccagg tgggtctgtt
     2101 gagcggcggg ccgacaccgc ttcccatgga cgcggtcatc gcccgcgagc tggagattct
     2161 gggcagccat ggtctggctg ccggcgatta tgcagagctg cttgccctcg tcgagtcggg
     2221 gcgagtcgac cccggcctcc tcgtcgaccg cgagatctca ttggaccagg tgcccgagga
     2281 actgatcgcg atgcgcacct tctcgggcga cggtgtcgcc gtggtgacga ggttctgaat
     2341 cagcacgggg cccggccgac cgggccacac cgtcacttgc ccgcatcgct gccgccgacc
     2401 gcgactatgg cagccatgac ctcgtcgaag tcggggagag tgagggcatc gtcactcgac
     2461 caggtgtagg tgatgcggcc gttgcggtcc atgacgaaga ccgatc
//
LOCUS       DATITW010000206         2744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_50154, whole genome shotgun sequence.
ACCESSION   DATITW010000206 DATITW010000000
VERSION     DATITW010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50154"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VLS86_02865"
     CDS             <1..578
                     /locus_tag="VLS86_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSO49464.1"
                     /translation="LTALLLAVAQRQERLRLSAVAGSVLAFAGVLLMSQVALDASISL
                     TSILVAMGAALCLAQGAVLVRIFPPAHPVSLNAVGMTVGAVILFIATLIAGDSFTMPT
                     LPETWLAIAYMVVIGSGVVFILWVFVLKRWEASRAAYNFVLLPPITLVFSSWILDEKV
                     GVELIFGGLLILVGVYIGALRPQRSPVEVPV"
     gene            686..1570
                     /locus_tag="VLS86_02870"
     CDS             686..1570
                     /locus_tag="VLS86_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49465.1"
                     /translation="MSVSLEQPLTTRADVLWPAPRHVYWLSMALALVAATSAALTFFV
                     PGILTGPAVTNGNARGTALVMLVAAVPVLLISMSLTARGSWRAPVVWLGALGYLLYNG
                     FLLLFLTPFNSLFLLYVATFSLCLFAIAGLVRVIDAPTLAARFRGIPRRGLALYVWTI
                     VFLNLLAWLRTIVPALGADDPSSFMEGSGVVTNPIFVQDMVFWFPMAALGAWWLWNGR
                     PWGVPVVGAWLVYGLLESIGVATDQWFGYRADPDTTFATMGGMWLFIALAVIGLIPLY
                     FFFRANRARKMGPEPRRQ"
     gene            1608..2216
                     /locus_tag="VLS86_02875"
     CDS             1608..2216
                     /locus_tag="VLS86_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49466.1"
                     /translation="MSRRSLFFVTVGLALVIAACGDDVTDTTSTTTGPTTTVAPTTTE
                     APTTTVPPATTTSASTTTSESDSDTNDLAEGSGCTPGGGPLPDGEWFGLVTSRGDDSI
                     EFDLACWFTGDAATQAAAEDGEESPPPNDYYVRNANPETREVPVASGAEVVFFPDGDP
                     NNEEVLAYADWAARVDERGYELGAWLEVEEGSITEIREQWVP"
     gene            2336..>2744
                     /locus_tag="VLS86_02880"
     CDS             2336..>2744
                     /locus_tag="VLS86_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSO49467.1"
                     /translation="MVLAGGAGNRLLPLTRDRAKPAVPFGGHYRLIDFALSNLVNGGH
                     RRIVVLTQYKSHSLNLHLSLTWRMSTLLGNYVTSVPAQMRLGERWFLGSADAIFQNLN
                     ILHDEHPEHVLVFGADHIYRMDPRQMLEQHLETG"
BASE COUNT          381 a          956 c          855 g          552 t
ORIGIN      
        1 cgttgacggc tctgctcctg gcggtggccc agcgccagga gagacttcgt ctctcagcgg
       61 tggcgggctc ggttctcgcc ttcgccgggg tgcttctcat gtcccaggtg gccctcgacg
      121 cttcgatctc gctgacctcg atcctggtgg ccatgggagc tgccctctgt ctcgcccaag
      181 gggcggtgct ggtgcggatc ttcccgccgg cgcacccggt gtcgctgaac gcggtgggca
      241 tgaccgtggg cgcggtgatc ctgttcatcg caacactgat cgccggcgac agtttcacga
      301 tgcccaccct tcccgaaacc tggctggcca tcgcctacat ggtggtgatc ggctccgggg
      361 ttgtgttcat tctctgggtg ttcgtcctca agcgttggga ggcgtcacgg gccgcttaca
      421 acttcgttct ccttcccccg atcacactgg tcttctcgtc ctggatcctt gacgagaaag
      481 tcggagtcga gttgatcttc ggcggtctgc tcatcctggt cggggtctac atcggcgccc
      541 tccgccccca gcgctcaccg gtcgaggtcc cggtctgaac cgacgccaag aaccggtaat
      601 ccccgccttc tgccggggac tttcggctct atgcgctccg ggctcgccgc caaatactga
      661 gactgaaatc tcacaaagga ggaccatgtc ggtctcgctc gaacagcccc tcaccacgag
      721 ggcagatgtg ttgtggcccg caccccgtca tgtctactgg ctgtcgatgg cgctggcgct
      781 cgtcgccgcg acctcggccg ctctcacctt cttcgtcccc gggatactca ccggccccgc
      841 ggtgaccaac ggcaacgccc gtggcactgc cttggtaatg ctggtggcgg cggtgccggt
      901 gttgctgatc tcaatgagtc tgaccgcccg cggctcgtgg cgggccccgg tggtgtggct
      961 gggcgcactc ggctacctgc tctacaacgg cttcctgctg ttgttcctga ccccgttcaa
     1021 ctcgttgttc cttctttacg tggcgacttt ctccctgtgt ctgttcgcga tcgcggggct
     1081 ggttcgggtg atcgacgccc cgacgctggc cgcccggttt cgggggatcc cgcgtcgggg
     1141 gctggctctc tacgtgtgga ccatcgtgtt cctcaacctg ctcgcctggc tgcggaccat
     1201 cgttcccgcc ctgggtgcgg acgacccgtc gtctttcatg gaggggtcgg gggtggtcac
     1261 caacccgatc ttcgtccagg acatggtctt ctggttcccg atggctgccc tcggcgcctg
     1321 gtggttgtgg aacggtcggc cgtggggggt tcccgtggtc ggagcctggc tggtgtacgg
     1381 cctcttggag tcgatcgggg tcgcaaccga ccagtggttt ggctatcgcg ccgacccgga
     1441 taccaccttc gccaccatgg gcggcatgtg gctgttcatc gccctggcgg tgatcggtct
     1501 catccccctc tacttcttct tccgggccaa ccgtgcccgg aagatgggcc cggagccacg
     1561 ccggcagtag cctcgaccct gtagcgcatt ctggatagga ggtctgcttg agccgccgtt
     1621 cgctgttctt cgtcactgtt ggtctggctc tggtgatcgc cgcctgtggc gacgacgtga
     1681 ccgacaccac gtccaccacc accgggccca cgacgacagt tgcccccacc accaccgagg
     1741 cgccgaccac caccgtccca cccgcaacca ccacatcggc ctcgaccacg accagcgagt
     1801 ccgactccga caccaacgat ctcgccgagg gctcgggatg cacgccgggg ggcggtccgc
     1861 tgcccgacgg tgagtggttc ggtctggtca cctcccgggg agatgactcg atcgagttcg
     1921 acctcgcctg ctggttcacc ggggacgccg caactcaggc cgccgcggag gacggcgagg
     1981 agtcgccgcc gcccaacgac tactacgtcc gcaacgcaaa tccggagacc agggaggtcc
     2041 cggtggccag cggcgccgag gtggtgttct tccccgacgg tgacccgaac aacgaggagg
     2101 tgctcgccta cgccgactgg gccgcccgcg tcgacgaacg tggctacgag ctcggtgcct
     2161 ggctcgaggt cgaagagggc tcgatcaccg agatccgcga gcagtgggtg ccgtgacctc
     2221 accctccttc ccgtaattgg gagcgccagg tccgtatatg acccctctcc gtcccaattt
     2281 gggagggggg attagctttg ccgcggtgag ctcggcccat ccccatgtgg tggcaatggt
     2341 cctcgccggt ggggcgggga accggctgtt gcccctgacc cgggaccggg ccaagccggc
     2401 cgtccccttt ggcggccact accggctgat cgacttcgct ctgtccaacc tggtcaacgg
     2461 gggccaccgg cggatcgtgg tgctcaccca gtacaagagc cacagtctca acctccatct
     2521 ctccctcacc tggcggatgt ccacgttgct cggcaactac gtcacctcgg tcccggctca
     2581 gatgcggctc ggagagcgct ggttcctcgg ctcggccgac gccatcttcc agaacctcaa
     2641 catcctccac gacgagcatc ccgagcacgt cctcgtcttc ggcgccgacc acatctaccg
     2701 catggacccg cgccagatgc tcgagcagca cctcgagacg ggtg
//
LOCUS       DATITW010000207         1986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_691117, whole genome shotgun sequence.
ACCESSION   DATITW010000207 DATITW010000000
VERSION     DATITW010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691117"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="VLS86_02885"
     CDS             complement(<1..1355)
                     /locus_tag="VLS86_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HSO49468.1"
                     /translation="MSPQVSRRNQGSTLQLLKTVAYVLGSILVFVAVAMLTAALISLI
                     NGELDTALWITVSAALTVAAGLVMRQLVPRPSSISVREGFVTVGLAWFVFSLFGAIPY
                     LLTGSIPSVTDAVFETASGFTTTGASILADPSLLPDGIAFWRGLTQWLGGMGVIVLGV
                     AVLPLLGTGGAQLAQAESPGPTLDRLTPRFQETAKRLWIVYAAITVVQIALLAFGDMD
                     MMQSVIHSFATMSTGGFGTEADSLAGFSNYTQWVITFFMFVAGVSFALHYRALSRPVE
                     YVRNSEFKLYLLITVAAIVVVAGGLWEDLAASEAVRHAAFNTVSIVTTTGFASTDFGL
                     WRPTLQIVLIGLMFVGGMAGSTAGAIKTFRIGVLSKAAYADLRRLIHPRGIFVTRFGG
                     AKVSDPVVEAVQSFFLFYMFLFMTSVFLLAFIDANIAEGLDLVTAASAVAASLGNIGP
                     GL"
     gene            complement(1352..>1986)
                     /locus_tag="VLS86_02890"
     CDS             complement(1352..>1986)
                     /locus_tag="VLS86_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HSO49469.1"
                     /translation="TRFAELAARELQKAGLSVVLVEEDKDRSNRLATKFPKALVVRGD
                     PTDPDLLRELDLGPEDVALGLSGWDEVNLLGCLVAKAIGAGMVISRFNRVAYVGLLTG
                     VGIDAAVSSRLLAASAILRFVRQGAIEQVAPFSDTDAEAIEIEVAEGAEAVGRTLLQL
                     ALPLGVVIGGISRQGTSFVPDGSTVIRPGDHIIFFSLPSEIEESAALFTA"
BASE COUNT          406 a          610 c          688 g          282 t
ORIGIN      
        1 agaccggggc cgatgttccc caatgaggcg gccacagccg atgctgcagt gaccaggtcg
       61 agaccctcgg cgatgttggc gtcgatgaag gcaaggagga agaccgaggt catgaagaga
      121 aacatgtaga agagaaagaa ggactggacc gcttcgacca ccgggtcgct caccttggcg
      181 ccgccaaacc gcgtgacgaa gatccctcgg ggatggatca accgccggag atcggcatag
      241 gccgccttgc tcaggacacc gatccgaaag gtcttgatcg cgcccgcggt ggagcccgcc
      301 atccccccga cgaacatgag cccgatgagg acgatctgga gggtgggacg ccacagaccg
      361 aagtcggtcg acgcgaaccc ggtggtggtg acgatggaca ccgtgttgaa ggcggcatga
      421 cggaccgcct cgctcgccgc caggtcctcc cagagcccgc cggcgaccac gacgattgca
      481 gcaacggtga tgagcaggta gagcttgaac tccgagttgc gcacatattc gacgggtcgg
      541 gacagggccc ggtagtggag ggcgaaggag acgccggcga cgaacatgaa gaaggtgatc
      601 acccattggg tgtagttgct gaagccggcc agcgagtccg cctcggtgcc gaagcctccg
      661 gtggacatcg tggcgaaaga gtggatcacc gactgcatca tgtccatgtc cccaaaggcg
      721 aggagggcga tctgcaccac ggtgatcgcc gcatagacga tccacagccg cttggccgtc
      781 tcctggaagc ggggggtcaa ccggtcgagg gtcgggcccg gcgactccgc ctgcgccagc
      841 tgcgccccac cggttccgag gagaggcagc accgcgactc cgagcacgat cactcccatg
      901 ccgcccagcc attgggtgag accccgccag aaggcgatcc cgtcgggcaa caggcttggg
      961 tcagccagaa tcgacgcccc ggtggtagta aagccggaag cggtttcgaa caccgcgtcg
     1021 gtcaccgagg ggattgaccc ggtgagcaga taggggatgg ctccgaacag ggagaacacg
     1081 aaccaggcga ggccaaccgt gacgaatccc tccctgacgc tgatcgagct gggccggggg
     1141 acgagctgtc gcatgacaag accggcggcg acggtgagcg cagcggacac cgttatccag
     1201 agggcggtgt cgagctcgcc gttgatcaga gagatcagag ccgcggtgag catggccacg
     1261 gcgacgaaga cgaggatcga cccgaggacg taagcgacgg tcttcaggag ctggagggtg
     1321 gatccctggt tgcgacgcga gacctggggg ctcatgccgt gaagagggcg gccgactctt
     1381 cgatctcgct gggaagcgag aagaagatga tgtgatcgcc cggtctaatc actgtcgagc
     1441 cgtcagggac gaacgacgtc ccctgacgtg agatgccgcc gatcaccacc ccgaggggca
     1501 gggcgagctg gaggagcgtc cggccgaccg cctcggcgcc ctcggccact tcgatttcga
     1561 tggcttcggc gtcggtgtcg gagaaagggg cgacctgctc gatggcaccc tggcgcacga
     1621 atcggaggat ggcgctggct gccaggagcc ggctcgacac cgcggcgtcg atgccgacac
     1681 cggtgaggag gccgacgtag gcaacccggt tgaatctgga gatgaccatc ccggcaccga
     1741 tggccttggc caccaggcac ccgagcaagt tcacctcgtc ccagccactg agccccagcg
     1801 ccacgtcctc agggcccagg tcgagctctc ggagaaggtc ggggtcggtc ggatcccccc
     1861 gcaccaccaa ggctttgggg aatttcgtgg ctagtcgatt ggaccggtcc ttgtcctcct
     1921 cgacgaggac caccgagaga ccggccttct gcaactccct ggcggcaagc tcggcgaagc
     1981 gggtcc
//
LOCUS       DATITW010000208         1656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_490708, whole genome shotgun sequence.
ACCESSION   DATITW010000208 DATITW010000000
VERSION     DATITW010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490708"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..599)
                     /locus_tag="VLS86_02895"
     CDS             complement(108..599)
                     /locus_tag="VLS86_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HSO49470.1"
                     /translation="MTVTVRSLRRAHDNTVLALDEIDKSIIRHLQEDGRMSYADLGPA
                     VGLSPAAARQRVLALLEAGAMQIVAVTDPTKMGFPVQAMVGFRVNGDLEAIAAEIGRL
                     PEVDYLVITTGRFDLLAEIVAEDNDHFLQVLSSIRSMPVVEASEAFTYLRLEKQSYDW
                     GAR"
     gene            709..>1656
                     /locus_tag="VLS86_02900"
     CDS             709..>1656
                     /locus_tag="VLS86_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSO49471.1"
                     /translation="MTTTATSIRLLTDIPGPKSRAILARREAATPQGNAKLTPVSIDS
                     AHGSVVIDVDGNHFIDLAGGIGVLAVGHTPERVVAALQEQAAKLIHMCAIVSTYEPFV
                     AVAERLNQLAPGDFPKKTVLMNTGAEAVETAVHIARAHQKRQGIVVFEGAFHGRTNLT
                     MSMTSKFNLFKKGFGPFAPEVYRFPFPNVYRRPPGMSEDAFVDWHLSLLDQSFVAQVD
                     PSHVAAIFVEPVQGEGGFVPAPFAWLRRLRELADEHGILLVSDEIQSGMGRTGTLWAI
                     DHSGVIPDMVTTAKSLAAGMPLSAVVGPASVMDAPHPGGL"
BASE COUNT          296 a          537 c          500 g          323 t
ORIGIN      
        1 cttcgaccgg gacccgttcc gggacggacc gatcggtgaa gcaacagttg tggcgaccct
       61 gatcggcgga gtggttgtct acgagagcga ctagtaaggc tgagacttta ccgggcaccc
      121 cagtcgtagc tttgtttttc gagtctcagg taggtgaagg cttcgctggc ttcgactacg
      181 ggcatgctgc ggatcgaact caggacctgg aggaagtggt cgttgtcctc ggccacgatc
      241 tcggcgagga ggtcgaagcg acccgttgtg atcaccaggt agtcgacctc gggcaggcgg
      301 ccgatctcgg cggctatcgc ctcgaggtca ccgttgaccc ggaaaccgac catcgcctgg
      361 accgggaagc ccatcttggt cgggtcagtc acggccacaa tttgcatcgc acccgcttcc
      421 agcaaggcca gcactcgctg gcgagcggcg gccggtgaca acccgacggc ggggccgagg
      481 tcggcatacg acatccgccc gtcctcctga agatggcgga tgatcgactt gtcgatctcg
      541 tcgagtgcga gaacggtgtt gtcgtgcgcc ctgcgaagcg atctaactgt tacggtcatt
      601 gcctggcccc attctcactt gcccttgtcg gaacggcaag cgatactgcc gtagatttag
      661 cataggacga gcgtttcgat tgcctctagt acctgggtga accgataaat gactacaacc
      721 gccaccagca tccgccttct caccgacatt cccggcccca agagccgtgc gatcctggcc
      781 cgtcgagagg ctgccactcc ccagggcaac gccaaattga ccccggtcag catcgactcc
      841 gcccacggtt cggtggtgat cgatgtcgac gggaatcact tcattgacct ggccggcggc
      901 atcggcgtcc tggccgttgg ccacactccc gagcgagtcg tggcggcgct gcaggagcag
      961 gcagccaagc tgatccacat gtgcgcgatc gtctcgacct acgagccgtt cgtggcagtc
     1021 gccgagcgcc tcaaccagct agcaccgggt gactttccca agaagaccgt cctgatgaac
     1081 accggggcgg aggccgtgga gaccgccgtc cacatcgccc gtgcccacca gaagcgtcag
     1141 ggcatcgtcg tcttcgaggg cgcgttccac ggtcgcacca acctcacgat gtccatgacc
     1201 tcgaaattca acctcttcaa gaagggcttc gggccttttg ctcccgaggt ctaccgcttc
     1261 ccgtttccaa atgtctaccg acgtccgccc ggcatgtccg aggatgcctt cgttgattgg
     1321 cacctcagcc ttctcgacca aagtttcgtc gcacaggtcg acccttccca tgtcgccgcg
     1381 atctttgtgg aacccgtgca gggtgaaggc gggttcgtgc ccgccccctt cgcctggcta
     1441 aggcggttgc gagaattggc cgacgagcac ggcatcctcc tggtgtccga tgagatccag
     1501 tccgggatgg gaaggaccgg aaccctctgg gccatcgatc actcgggcgt catccccgac
     1561 atggtcacca ccgccaagtc actggcggcc gggatgccgc tctcggctgt ggtcgggcct
     1621 gccagcgtga tggatgcccc tcatcctggt ggactc
//
LOCUS       DATITW010000209         1812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_490713, whole genome shotgun sequence.
ACCESSION   DATITW010000209 DATITW010000000
VERSION     DATITW010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490713"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VLS86_02905"
     CDS             complement(<1..592)
                     /locus_tag="VLS86_02905"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSO49472.1"
                     /translation="MTSAVAPLRYGSFRTLWVASVLSAVGTFIQSVASSWLMFELTRS
                     NTWVGMMVASTTLPLLFFALAAGALADMFDRTKLMLLAQGIMGGSALAMTILTALGSI
                     TPNLLLGLGLLLGVGVALNLPTWQALLPDLVPRGMIASAVALQSAAFNVARAIGPAIG
                     GALVVAVGPAAGFGINTLTYGFVIAALLIVGRSLTAR"
     gene            625..1260
                     /locus_tag="VLS86_02910"
     CDS             625..1260
                     /locus_tag="VLS86_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSO49473.1"
                     /translation="MVRDLAYFISGDRGPGPGVLVLHSFWGLTPSVKSLCDDLADHGF
                     TVLAPDINFGELPASEQEALDHLGKADPNRLASLVLSSAKLLHEKSTADLIGIVGFGM
                     GGSLALWASVRLANLVGAAVSFYGSQQIDFAGANAAYLIHLSEEDEYVTDDEAVFMEA
                     TMRLENLEVDVIRHANTRHGFAEPDGDAFDPSAFDKAWAGTLEFLERHLLR"
     gene            complement(1257..>1812)
                     /locus_tag="VLS86_02915"
     CDS             complement(1257..>1812)
                     /locus_tag="VLS86_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016362564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO49474.1"
                     /translation="DIYWNEELVEDPASFFVPPRSAYTAQVPRLFSESLADNIALGKR
                     ATRERLREAVQLAVLDPDLERMEHGLETMVGARGVKLSGGQVQRSAAARMLATEAELL
                     VFDDLSSALDLHTETELWRRLLTQRDVTCIVVSHRRAALQRADQILLMDRGRVVDRGG
                     LSDLLGRSDLMCSLWEEAGEEALS"
BASE COUNT          332 a          592 c          599 g          289 t
ORIGIN      
        1 cgcgagcggt cagcgacctc cccacgatca gcaacgccgc gatcacgaat ccataggtga
       61 gcgtgttgat gccgaatccg gcggcggggc cgacggcgac caccagggct ccaccgatcg
      121 caggcccgat cgcccgggcc acgttgaagg cagccgactg aagggccacg gccgaggcga
      181 tcatccccct cgggacgagg tcggggagga gcgcctgcca ggtggggagg ttgagcgcca
      241 cccccacgcc caggagcaaa cccagaccga ggaggagatt tggcgtgatc gagcccaagg
      301 cggtgaggat ggtcatcgcc agggcggagc cacccatgat cccctgagcg aggagcatca
      361 gcttcgttcg gtcgaacatg tcggcgaggg cgccggcggc caacgcgaag aagagaagcg
      421 gcaaggtcgt cgacgccacc atcatcccca cccaggtgtt ggatcgggtg agctcgaaca
      481 tgagccacga cgaagccacg ctctggatga aggtgcccac cgcggaaagg accgatgcga
      541 cccaaagcgt gcggaaggag ccatagcgga gtggggcgac cgcggaggtc atcgttacca
      601 gcgtaggccc ggtattttcg tgtcgtggtt cgtgatctcg cctacttcat ctccggggac
      661 cggggaccgg gcccgggggt gctggtgctc cactccttct ggggcctgac cccatcggtc
      721 aagagcttgt gtgacgacct cgccgaccac ggattcacgg tcctcgcccc cgacatcaac
      781 ttcggcgagt tgccggcctc ggagcaggag gcgctggacc acctgggcaa ggccgacccc
      841 aatcgtctgg cctcgttggt gctgtcctcg gccaagctgc tgcatgagaa gtcgaccgca
      901 gacctcatcg gaatcgtcgg attcggcatg gggggatcgc tggcgctctg ggcgtcggtc
      961 cggctggcca acctggtcgg ggccgccgtc tcgttctacg ggagccagca gatcgacttc
     1021 gccggggcca acgccgccta tctgatccac ctctccgagg aagacgagta cgtcaccgac
     1081 gacgaggcgg tcttcatgga ggccacgatg cgtctcgaga acctggaggt ggacgtgata
     1141 cgacacgcga acaccaggca cgggttcgcc gagcccgacg gcgacgcatt cgacccttcc
     1201 gccttcgaca aggcctgggc cgggacgctc gagtttctcg agcgtcatct gctccgttag
     1261 ctcagggctt cctcgccggc ttcctcccac agcgagcaca tgagatccga tcggcccagg
     1321 agatcgctca gccctccgcg gtcgacaacc cttccccggt ccatgaggag gatctggtcg
     1381 gctcgctgca gagccgccct gcgatgggag accacgatgc aggtgacgtc gcgctgtgtc
     1441 agcaggcgcc tccacaactc ggtctcggtg tgcaagtcga gggccgagga gagatcgtcg
     1501 aagacgagga gctcggcttc tgttgccagc atcctggccg cggccgaacg ctgcacctgc
     1561 ccaccggaca gcttcacccc tcgggcaccc accatggttt cgaggccgtg ctccatccgc
     1621 tccagatctg gatcgagtac cgccagttga acggcctcgc gcaggcgctc tcttgtggcc
     1681 ctcttaccga gagcgatgtt gtcggccagc gactccgaga agagacgcgg cacctgggcc
     1741 gtataggcag aacggggcgg cacgaagaag gatgccggat cttcgacgag ttcctcgttc
     1801 cagtagatgt ca
//
LOCUS       DATITW010000210         4870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_611051, whole genome shotgun sequence.
ACCESSION   DATITW010000210 DATITW010000000
VERSION     DATITW010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611051"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2284)
                     /gene="secD"
                     /locus_tag="VLS86_02920"
     CDS             complement(<1..2284)
                     /gene="secD"
                     /locus_tag="VLS86_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HSO49475.1"
                     /translation="MPKVSRWRLFLTVMSIGAAVYIVLNNPINLGLDLRGGTQIVLEA
                     QDTPEVTVDSEVTGRTLEVLRRRVDAFGVSEPTLQISGDRRIIIELPGLDDPDQALEV
                     IGRTAQLTFHPVLDSVPTGTAAEDDDALVLPGELGEDVVLGPAALTGDEVESALSIFD
                     SQGSGLWAVSIDFRPDGEQAWAELTAEAACYPQGDPRRRVAIVLDDTILSSPSVSPSV
                     QCDVGITGGGTLITGNFDEDSAGELALLVRAGALPVPVVVIEQGTIGPSLGEAAIAAS
                     LQAALIGAGLTIIYMVAYYRLMGAAAALSLGVYALLSYAVLAWMGATLTLPGIAGFVL
                     AIGMAVDANVLVYERAKEEYALEPSLGSSILAGFQRAWSAIADANVTTLLAAILLFFF
                     ASGAVRGFGVTLTVGVLVSMFSALVVTRVLLELLARIPALQARPNLMGMSVGRRLQNW
                     LKTSGLNLFANPRRWLVISGIAVLIAIAGAIVQGLNFGLEFLGGRLVEFSTTQTVDLE
                     ELRQDLTDLGLPRALIQETGEGNVVIRTEQLDADQEQLLEDTVAGIGGEVTVVRDQFV
                     GPTLGREIRNRALIALGLALLLQLGYLAIRFRWTIGLAAVAAMFHDVIIVIGVFAWLG
                     RTLDGVFLAALLTVIGYSINDSVVIFDRIREQRAARPDEPLSRIANDACLQTVPRTIN
                     TGLGAMMILLALFFLGGDTLADFALDLLIGILIGTYSSVFTATPIAIALEDRFPGPPP
                     EPVSKDTRRPHDKQKPKSGDR"
     gene            2414..3265
                     /gene="panB"
                     /locus_tag="VLS86_02925"
     CDS             2414..3265
                     /gene="panB"
                     /locus_tag="VLS86_02925"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HSO49476.1"
                     /translation="MREKVTVPAIRARKGGPKITMVTAYDYPGAVIVDRAGVAILLVG
                     DTAAHVVHGMATTLELGMDEIIMHSRAVRRADTLSLFVVDMPWLSFHISPEETVRNAG
                     RIVQEGGAEAVKIEGGRKRLPVIERILDAEIPVMGHLGLTPQSVHTMGGFKVQGKMVD
                     AAQEMIEDARALADAGCFSIVIEGVPDILGELITKEVDVPTIGIGAGPATDGQVLVFH
                     DVLGLGSGRYPKFVRSYANLADEAVAALERYVADVSSGDFPGEDESYHVSEEIAAEML
                     KGRQSES"
     gene            3277..3681
                     /locus_tag="VLS86_02930"
     CDS             3277..3681
                     /locus_tag="VLS86_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HSO49477.1"
                     /translation="MVACASSTTGGSDVPTQSVSVGDAELTVWVADDPDERRQGLRGV
                     EQLPPGVDGMLFVFPEPATPSFGMLGALLELDLWFFDDQGVLIGSEEMAPCAAEPCPR
                     YPAPGEVRWALETAQGSWELEPGDVLTTSAIG"
     gene            3792..4079
                     /gene="rpsF"
                     /locus_tag="VLS86_02935"
     CDS             3792..4079
                     /gene="rpsF"
                     /locus_tag="VLS86_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HSO49478.1"
                     /translation="MRDYELMTIHRPDLAEDEIAAKIGELESHLSGSGATVAGRDLWG
                     KRRFAYEIDHLSEGYYSVITFQAEVAAVDSLDRMLSLADEVIRHKIVRKGD"
     gene            4142..4600
                     /gene="ssb"
                     /locus_tag="VLS86_02940"
     CDS             4142..4600
                     /gene="ssb"
                     /locus_tag="VLS86_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSO49479.1"
                     /translation="MSNNVTLIGNLVDDPELRFTPSGVAMAKIRMAVNRRWQKDGEWQ
                     EDTSFFTGTVWREQAESAAESLQKGARVIVTGRLEQRSWETDQGEKRSVVEVQIDEIG
                     PSLRWATATVNKTQRSGGEWGGGQGGGGQAGDVPPAPVARDNYGPDEAPF"
     gene            4617..>4870
                     /gene="rpsR"
                     /locus_tag="VLS86_02945"
     CDS             4617..>4870
                     /gene="rpsR"
                     /locus_tag="VLS86_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HSO49480.1"
                     /translation="MARKSTNNPRPKRRRGVEVRRGRPKPCAFCKQRIDHIDWKDVAV
                     LRKYMSDRGKIRGRRVNGNCPTHQRAVANAIKNAREMALLP"
BASE COUNT          901 a         1532 c         1658 g          779 t
ORIGIN      
        1 tcctgtctcc cgactttggc ttctgcttgt cgtgtggacg ccgggtgtcc ttggacaccg
       61 gctcgggggg cgggccgggg aacctgtcct cgagggcgat cgcgatcggt gtcgcggtga
      121 acaccgacga gtaggtgccg atcaggatcc cgatgaggag gtcgagcgca aagtcggcga
      181 gggtgtcccc acccaggaag aacaaggcga ggaggatcat catcgccccc aacccggtgt
      241 tgatcgtccg cggcaccgtc tgcaggcacg cgtcattggc gatgcgcgac agcggttcat
      301 cgggccgggc ggcccgttgc tcgcggattc ggtcgaagat cacgaccgag tcgttgatcg
      361 agtagccgat cactgtcagg agcgcggcga ggaacacacc gtccagcgtt cgtcccagcc
      421 aggcgaagac gccgatcacg atgatcacgt cgtggaacat cgcggccaca gcggcgagtc
      481 cgatggtcca ccggaagcga atcgccagat agccgagttg cagcaggagt gccagcccca
      541 acgcgatcag ggcccggttg cggatctccc gccccagggt gggcccgacg aactggtccc
      601 ggaccactgt gacctccccg ccgatcccgg cgaccgtgtc ctcgagcaac tgctcctggt
      661 cggcatccag ctgctcggtc ctgatcacca cgttgccctc acctgtctcc tggatcaagg
      721 cccggggcag cccgagatcg gtcaggtcct gtcgcagctc ctcgaggtcg acggtctgtg
      781 tggtcgagaa ctcgaccaac cggccgccca ggaactcgag gccaaagttg agcccctgga
      841 cgatggcccc ggcaatggcg attagcaccg cgatgccgga gatcaccaac cagcgtcgcg
      901 ggttggcgaa caggttgagc cccgaggtct tcaaccagtt ctgcagtcgc cgccccacgc
      961 tcatgcccat caggttgggc cgagcctgaa gcgccgggat ccgcgccagc agctcgagga
     1021 ggacccgggt caccaccaac gccgagaaca tggagaccag gacgccaacg gtgagggtca
     1081 caccaaagcc tctgaccgcg ccggaggcga agaagaagag aaggatggcg gcgagcaggg
     1141 tcgtcacgtt ggcatcggct atcgcgctcc aggcacgctg gaacccggcc agtatcgagc
     1201 tgcccaggga cggctccagg gcgtactcct ccttggcccg ctcgtagacg agcacgttgg
     1261 cgtcgaccgc catgccgatg gcgaggacga aaccggcgat cccgggcagg gtcagggtgg
     1321 ctcccatcca ggcgaggacg gcgtaggaga gcagggcgta gacccccagc gacaatgcag
     1381 ccgccgcgcc catgagccgg taataggcga ccatgtagat gatggtgaga ccggccccga
     1441 tcagcgccgc ctggaggctg gcggcgattg ccgcctcacc cagggacggt ccgatggtgc
     1501 cctgctcgat gaccacgacc gggaccggaa gcgctccggc acggaccagc aaagcgagct
     1561 ctccggcgct gtcctcgtcg aagttccctg tgatcagcgt ccctccaccg gtgatgccga
     1621 cgtcacactg aaccgacggc gatacggagg gactggagag gatcgtgtcg tcgaggacga
     1681 tggcgaccct tcgccgcggg tcaccctggg ggtagcacgc cgcctcggcc gtgagctcgg
     1741 cccaggcctg ttccccgtcg ggacgaaagt cgatcgatac cgcccacaac ccgctgccct
     1801 ggctgtcgaa gatggaaagg gcgctctcca cctcatcgcc ggtgagggcg gccggtccca
     1861 ggaccacgtc ctcaccgagc tcaccgggaa ggacgagggc atcgtcgtcc tcggctgccg
     1921 tcccggtagg caccgagtcg agcacgggat ggaaggtgag ttgggcggtt cgcccgatca
     1981 cctccagggc ctggtcgggg tcgtcgagac cgggcagctc gatgatgatc cggcgatccc
     2041 ccgagatctg cagtgtgggc tcggagacac cgaaggcgtc gacacggcga cggaggactt
     2101 caagagtgcg accggtcact tcgctgtcga cggtgacctc gggggtgtcc tgggcttcga
     2161 ggacgatctg agtgccacca cgaaggtcga gaccgagatt gatcgggttg ttgaggacga
     2221 tgtagacggc agcacctatc gacatcaccg tcaagaagag gcgccaccgg gacactttgg
     2281 gcatagcctt cccttcgagg cccggagcaa tctccgcatg ctatcgggcg ctatccggca
     2341 tgccagatcg caaccgccga aaccaggagc gcgagccaaa accgcaatgg tcgccacccg
     2401 ttatccttct ccaatgcgcg agaaagtcac tgtcccggcg attcgtgctc gcaagggcgg
     2461 gcccaagatc acgatggtca ccgcctacga ctacccgggt gcggtcatcg tcgatcgggc
     2521 cggcgtcgcc atactcctgg tcggggacac tgccgcccac gtggtccacg gtatggcgac
     2581 gacactcgag ctcgggatgg atgagatcat catgcattcc cgggcagtga gacgggccga
     2641 caccctctcc ctgttcgtgg tcgacatgcc ctggctcagc tttcacatct cacccgagga
     2701 aaccgttcgc aatgcgggcc ggatcgtcca ggagggcggg gcggaggcgg tgaagatcga
     2761 aggtggccga aagcgactgc cggtgatcga gcgcatcctc gacgcagaga tcccggtgat
     2821 gggccacctc gggctcaccc cgcaatcggt gcacaccatg ggcggcttca aggtccaggg
     2881 gaagatggtc gatgccgccc aggagatgat cgaggacgcc agggccctcg ccgacgccgg
     2941 gtgcttctcg attgtgatcg aaggagtgcc cgacatcctc ggcgagttga tcacaaagga
     3001 ggtcgacgtc cccacgatcg ggatcggcgc cgggccggcg accgacgggc aggtcctcgt
     3061 cttccacgat gtgctcggtc tcgggtcggg tcgctatccc aagttcgtcc gcagttacgc
     3121 caacctcgcc gacgaggccg tggccgcgct ggagaggtat gtcgccgatg tgagctcggg
     3181 tgatttcccc ggtgaggacg agagctatca cgtgtccgag gagatagccg ccgagatgct
     3241 gaagggtcga cagtccgagt catagccctg ctcctggtgg tggcatgtgc ctcgtcgacc
     3301 acgggtggct ccgacgtccc gacccaatcc gtgagtgtcg gcgacgccga gctgacggtg
     3361 tgggttgccg acgaccccga tgagcgtcgg cagggcctgc gtggcgtgga gcaactgcca
     3421 ccgggggtgg atggcatgct gtttgtcttc cccgagcccg ccactccgag cttcgggatg
     3481 ctgggggcgc tgctcgagct cgacctctgg ttcttcgacg accagggagt gctcatcggc
     3541 tccgaagaga tggcgccctg cgctgccgag ccttgtcccc ggtacccggc tcccggagaa
     3601 gtgcgttggg cgctggagac ggcgcagggt tcgtgggagc tcgagccggg cgatgtcctc
     3661 acgacttcgg caatcggctg atatccccta ccattgcgca tccgcagcga cgtctcgcgc
     3721 cgttcgccgc gtgaagcccc tgctccaccg ggttggtggg gccttccaat cggatgtcca
     3781 caggaggtca catgcgcgac tatgagttga tgacgatcca tcggcccgat ctggccgagg
     3841 atgagatcgc agccaagatc ggcgaattgg agtcacatct ctccgggtcc ggggcaactg
     3901 ttgccgggcg ggacctgtgg ggtaagcgac ggttcgccta tgagatcgat catctatccg
     3961 agggctatta ctcggtgatc acgttccagg cggaggtggc tgccgtcgac tcgctcgatc
     4021 gcatgctgtc cctggccgac gaggtgatcc gacacaagat cgttcgcaag ggcgactgac
     4081 tggaatccgt cccagcggcg cggcatgatc gacgcaggga cgtgagtaga ggagatttcg
     4141 aatgtcgaac aacgtcacat tgataggcaa cctggtcgac gacccggagt tgcgcttcac
     4201 cccctcaggg gtggccatgg ccaagatccg catggcggtc aaccgtcgtt ggcagaaaga
     4261 cggcgaatgg caggaagaca ccagcttctt caccgggacc gtctggcggg agcaggctga
     4321 gagcgctgcc gagtctctgc agaagggggc cagggtgatc gtgaccggtc gtctcgagca
     4381 gaggagctgg gagaccgacc agggggagaa gcgctcggtg gtcgaggtcc agatcgacga
     4441 gatcgggcca tcgctccgct gggcgacagc caccgtgaac aagacccagc gctccggcgg
     4501 tgaatggggt ggcggacagg gtggaggcgg tcaggccgga gatgtcccgc ccgcaccggt
     4561 ggcacgcgac aactacgggc ccgacgaggc gcccttctga tcaggagtct gagctaatgg
     4621 caagaaagag caccaacaac ccgcggccca agcggcgtcg cggcgtggaa gtgagacgtg
     4681 gccgtcccaa gccatgcgcc ttctgcaagc agcgcatcga tcacatcgac tggaaggatg
     4741 tggcggtcct ccgcaagtac atgtccgacc ggggcaagat ccgggggcgg cgggtcaatg
     4801 gaaactgccc gacgcaccag cgggccgtgg ccaacgcgat caagaatgcg cgggagatgg
     4861 ccctgcttcc
//
LOCUS       DATITW010000211         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_571168, whole genome shotgun sequence.
ACCESSION   DATITW010000211 DATITW010000000
VERSION     DATITW010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571168"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VLS86_02950"
     CDS             complement(<1..274)
                     /locus_tag="VLS86_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49481.1"
                     /translation="MTVSAPWERRVHFSVEVGQFIALVGAIVVDLVSTGGHSESLFAM
                     GVATIYVLGSAAVPEEWYHIRFGAEAITLMGAFLTVVAITLTGGPSS"
     gene            299..880
                     /gene="coaE"
                     /locus_tag="VLS86_02955"
     CDS             299..880
                     /gene="coaE"
                     /locus_tag="VLS86_02955"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HSO49482.1"
                     /translation="MTTENRRWLLSGGIGSGKSEVGRLLAATGIRVVDADRMGHRVLE
                     PSGRAFGAVADRWPEVLRDGVIDRGRLASIVFADPGQLRELEAVTHPHIFGTIRDELE
                     GFEELAVVEVPIIDTGLVWPRMVVDAPDEIRFERALERGMNTDDLRARMASQPNRAQW
                     LASADLVIPNHGALEELTETVAEVLHLVKGNAG"
     gene            921..2046
                     /locus_tag="VLS86_02960"
                     /pseudo
     CDS             921..2046
                     /locus_tag="VLS86_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512502.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT          391 a          629 c          676 g          350 t
ORIGIN      
        1 ggctggaggg cccacccgtc agggtgatgg cgactacggt caggaaggcc cccatcaagg
       61 tgatggcctc ggcgccgaat ctgatgtgat accactcttc gggcactgcg gccgacccca
      121 ggacgtagat ggtggcgacg cccatggcga acaaggactc gctgtggccc ccggtgctca
      181 ctagatcgac gacgatggca ccgaccaggg caatgaactg gccaacctcg acactgaagt
      241 ggacgcgtcg ctcccaagga gcgctgacgg tcatagggaa aggctaccgt cgggcgaggt
      301 gactacagag aaccgtcgtt ggctgctgtc gggtggaatc ggctccggaa agtccgaagt
      361 gggccgtctc ctcgccgcca ccgggatccg cgtcgtcgac gccgaccgga tgggccatcg
      421 ggtgttggag ccgtcaggcc gtgcatttgg ggcggtggcc gaccgctggc cagaggtgct
      481 gcgcgacggc gtgatcgata gagggcggtt ggcaagcatc gttttcgccg atccgggtca
      541 gctccgggag ttggaggccg tgacccaccc ccacatattc ggcacgatcc gggatgaatt
      601 ggagggtttt gaggagctgg cggtcgtcga ggtgccgatc atcgacaccg gtctggtttg
      661 gccccgaatg gtggtcgacg cccccgacga gatccgattc gaacgcgcat tggaacgggg
      721 catgaacacc gacgaccttc gagcccgaat ggcctcccag cccaacaggg cgcaatggct
      781 ggcctcagcc gatctggtga ttccgaacca cggggccctg gaggagctga cggagacggt
      841 ggccgaggtg ttgcatttgg tgaaggggaa cgccggatag cgggcagtcc ttgtcccacc
      901 ctcccggtac cctccgtttc gtgcccgagt tcaagctcgt ctccgacttc gcccccgccg
      961 gggatcagcc acaagccatc gagaagctgg ccgaggggat caatcgcggc gatcggttcc
     1021 agacgctgct cgggatcaca gggtcgggaa agtcggccac tatcgcctgg accatcgaac
     1081 gggtgcagaa gccgaccctg gtcatcgctc ccaacaaggc actaggagcc cagctggcca
     1141 acgagttcag gcagttcttc cccgagaacc gggttgagta cttcgtctcc tactacgact
     1201 actaccagcc cgaggcctac atccctcaga ccgacaccta gatcgagaag gacgcaacga
     1261 tcaacgatga gatcgaccgg ctccggcatt ccgccaccgc gggactgctg gtccgtgacg
     1321 atgtgatcat cgtggcctcg gtgtcggcga tctacgggct gggctcgccc gagcagtacc
     1381 tgggtcaact gcttcgtctg atccggggag tggagtaccc gatgacggcg gcgatcaagc
     1441 gcctggtcga catccagtac gagcgtaacg aggtgaacct ggttcggggc aagttccggg
     1501 tccggggaga caccctcgag gtgttcccct cgtatgagga aaccatcgtc cgggtccaat
     1561 tctggggaga cgagatcgag cgcatcctcc ggctcaaccc ggtcaccggg gaggtgatcg
     1621 acgagatgtc ggagctgatg atcttccccg ccacccacta cgtcaccgag cgggatcgga
     1681 tgttgaaggc gatcgacggc atcgaggtcg agttggccga ccggctggcc gatctcgaga
     1741 gccagggcaa gctgttggag gcgcagcggt tgcggatgcg gaccaactac gacctggaga
     1801 tgatgcgaga gatcgggttc tgctcgggga tcgagaacta ctcacgtcac ctcgacgggc
     1861 gctcgccggg tgaggcgccc tacacgctcc tcgactactt ccccaaggac tacctcacca
     1921 tcatcgacga gtcccatgtc accgtgcccc agatcggtgg gcaattcgag ggagatcgct
     1981 cccgcaagga agtcctggtc gagcacgggt tccggctgcc ctcggctaag gacaaccggc
     2041 cgcttc
//
LOCUS       DATITW010000212         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_290764, whole genome shotgun sequence.
ACCESSION   DATITW010000212 DATITW010000000
VERSION     DATITW010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290764"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="VLS86_02965"
     CDS             <1..1482
                     /locus_tag="VLS86_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49483.1"
                     /translation="DEDNASPVASIVLIVDGLPLAIELAAARLRMLSPKALCDRLTRS
                     LDALGAGAADVPTRQRTLDAAIDWSYQLLTREEQAFFARLCVFSGGFTVESAQDVAVT
                     EGDAVDQLMVLVENSLVLPAHGGEGRLRMLAPIREFGLRRLHESGEYDRVKDRHAGYY
                     VRVAEGSLHDLRGVKQAEAVERLAADWNNIDVAADWLMGKGDHASLVTLTYGLWVFLW
                     VGNHLRDGRRWLDAVTGPEGLEPMLAGRFWWLKGGISYEMGEYSKSEEAIDLALALLE
                     VNGDVDCHNWAEFLSTLLIPAFGGEVEQTREGLEVSLSRFRGFGDRWGEGYALIGLGI
                     LAGSSGDAALAESYQLETRDLGFAMGNSALIGLAEAQMGFTYVGSGRLEEARQSLRRA
                     FDVFRDMNYREGLCYALEAAASLSFSEGRAELGMIALGAAEEVRARIGLHPWPLIMWL
                     FESLSAMADSLDEPALQAARLNGRQMNPFDAAALVLDAAPVPA"
     gene            1569..1997
                     /locus_tag="VLS86_02970"
     CDS             1569..1997
                     /locus_tag="VLS86_02970"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HSO49484.1"
                     /translation="MTQQETALPAETVERPPTRLRRSRDDRVIAGVCGGLGRYFNTDA
                     VWFRLGFVVLTVAGGAGLLIYLIAWLIIPEAAPGESLESGPTDIGAQGPVVAGVALVG
                     LGLMLLFNTLVPWFDQVMWPLAVIAAGTGLLYLGSRREHN"
BASE COUNT          319 a          650 c          756 g          383 t
ORIGIN      
        1 gacgaggaca acgcctcacc ggttgcctcc atcgtgctaa tcgtcgacgg acttcccctc
       61 gccatcgagc tcgccgcagc ccgtctccgg atgctcagcc caaaggccct ttgtgatcgg
      121 ctgacccggt cgctggatgc actgggggca ggcgccgccg acgtccccac ccggcagcga
      181 accctggatg cggcgatcga ctggagctat cagctcctga cccgggagga gcaagccttc
      241 ttcgccaggc tctgcgtgtt cagcggtggc ttcaccgtcg agtcggccca agatgtcgca
      301 gtgaccgagg gcgatgccgt cgaccagttg atggtgctcg tcgagaacag cctggtgctg
      361 ccggcgcacg gcggcgaagg ccggcttcga atgctggccc cgatcaggga gttcggcctc
      421 cggcgcctcc acgagtcggg tgagtacgac cgggtcaaag accgacacgc cggctactac
      481 gtccgcgtcg ccgaaggatc gcttcacgac ctgcgcgggg tgaagcaggc ggaggctgtg
      541 gagcggctgg ccgccgactg gaacaacatc gacgtggcgg cagattggtt gatgggcaag
      601 ggcgatcatg cgtcgctcgt caccctcacc tacgggctgt gggtcttcct ctgggtcggc
      661 aaccatctgc gggacggaag acgatggctg gacgcggtca ccggccccga ggggctcgaa
      721 ccgatgctcg ccggccggtt ctggtggctg aagggtggga tctcctatga gatgggcgag
      781 tactcaaagt ccgaggaggc aatcgacctg gcgctggcat tactcgaggt gaacggcgat
      841 gtcgactgtc acaactgggc cgagttcctc tccaccctgc tcatcccggc gttcgggggc
      901 gaggtcgagc agaccaggga ggggctcgag gtctcgctgt cccggttccg tgggttcggt
      961 gacaggtggg gagagggcta tgccctgatc gggctgggca tcctcgccgg gtcgagcggt
     1021 gatgctgcgt tggccgagag ctaccagttg gagacccgcg atcttggctt cgccatggga
     1081 aacagcgcgc tgatcgggct tgccgaggca cagatggggt tcacctacgt ggggtcggga
     1141 agactcgaag aggcgcgcca gtcgctgcgc agggctttcg acgtgttccg ggacatgaac
     1201 tatcgcgagg gcctctgtta cgcgctggag gcggcggcgt cgctctcgtt cagcgagggt
     1261 cgggctgagt tggggatgat cgccctgggt gcggccgagg aggtccgagc ccggatcggc
     1321 ctccatccct ggccgctgat catgtggctg ttcgagagcc tctccgccat ggccgactcg
     1381 ctggacgagc ctgcgcttca ggccgcccgt ctcaacgggc gccagatgaa tccgttcgac
     1441 gccgcggccc tggtgctgga cgcggctcct gttcccgcct gactcgcggc cttcccgccg
     1501 acgggtgact ttcgcctctg gtcgggttcg ggctgtggcg gcattgtcgg gctgaaggga
     1561 ggttgcgtat gacgcaacaa gagaccgccc tgcctgccga gaccgtcgag agaccgccaa
     1621 cgcggcttcg gcggagcaga gacgaccgag tgatcgccgg tgtctgcggt gggttggggc
     1681 ggtatttcaa caccgatgcc gtgtggttca ggttgggttt cgtggtcctc accgttgcgg
     1741 gtggggcggg ccttctgatc tacctgatcg catggctcat catccccgaa gccgcaccgg
     1801 gcgagagcct ggagagcggg ccaaccgaca tcggggcgca agggccggtg gtcgccggtg
     1861 ttgcgctcgt cggtctgggg ctgatgcttc tgttcaacac actggtgccc tggttcgacc
     1921 aggtgatgtg gccgctggcc gtcattgccg ccggcaccgg actgctctac ctcgggagtc
     1981 gccgtgagca caactgacac gctggagaga cccgggaccg agacggcccc gactcagaag
     2041 acgcggccct acggggcaat cgtctccgga gcactgctgg tggtggtcgg cggcctctgg
     2101 ctgctcga
//
LOCUS       DATITW010000213         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_290781, whole genome shotgun sequence.
ACCESSION   DATITW010000213 DATITW010000000
VERSION     DATITW010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290781"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            605..1234
                     /locus_tag="VLS86_02975"
     CDS             605..1234
                     /locus_tag="VLS86_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49485.1"
                     /translation="MDRGVLQEEVYVPVAVDAERVIEGVQMVRATYFVVRSDAPMGFW
                     QLELPPFASLRGVGAIGFFGVVYGALRGGGSAGIDLFSSSEDVAAIFALEVEGLPLSV
                     DLEDVWIPKSWIVAAQGGDESTASSDDELARGEVFRVALPVFQQVYRYTAGDLDLIEL
                     LSTEASGQVHHSPEETNALHAWSAEQIEITRQAFPSKTVKLRYREQGSA"
     gene            1227..>2029
                     /locus_tag="VLS86_02980"
     CDS             1227..>2029
                     /locus_tag="VLS86_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSO49486.1"
                     /translation="MPELAVTMLDVGWGDCILVDSRDDSGDRRFGLIDCNDFERQRTA
                     LPFVMRYFERLGIDWKARSHNFEWVLLTHGHADHARGLKEMMSTFGTRHFWYPKSVAS
                     TTYGVLLDFANRSSRVLHHQAVDQTKILDAPEVTFHADLRILWPNFDQIDTRNENNNS
                     VVLAVTLGQSTIVLTGDAEAENWPAIVPRLPATTTYLQVPHHGGRNGVFDPNESTPWL
                     DGISYRKTRLVMSSHIVPHGHPHPDVVSVLAARQFVSYRTDRNYDITLST"
BASE COUNT          371 a          640 c          630 g          388 t
ORIGIN      
        1 ggcatcttca atcacggcga ttaccggccg gggaggttgg gagagctctt cgagaaacgc
       61 gtcgaaagct ccctgcccgt cacccgcctg caaagtgtct tgaagggacc ctccgcatat
      121 ctgagcgatg tcggtcaggg gtcctagagg ccttggggcg aacaagtcat cacaacgccc
      181 aagcaacacc cgagagtcgt gtgcatagtc atggcagaag gcttcgacca gtgatgtctt
      241 accgaccccc gcctcgccac tgatcaagac cagctggccg ccgctatcaa cgctcgccag
      301 tcgctgtgcg atgattgcaa gcgagccggc tcgttctaag agcatggcca cgatgatggt
      361 agcgatgggc tgccggccga agccgtgcta tttctgagtc agcgacgccg accccgccta
      421 ccgacccagg cggttgactt ggtctggacg gccgtcgagc aattggccga cggccgggcg
      481 ctccgattat gcggttgcgg gtacggatct cagggaagag tccgggggcg tggctcgaag
      541 gggtaagtgt caccatcctc cgtctctggg cgtcgtcagg tggtgccggc gtagattcac
      601 cgcaatggac cggggtgtcc ttcaggagga ggtctatgtc cccgtcgccg tggatgcgga
      661 acgggtgatc gaaggtgtcc agatggtccg ggccacgtac ttcgtggtca ggtcggacgc
      721 cccgatgggc ttctggcaac tcgagcttcc accgttcgca tcgctgcgcg gcgtcggtgc
      781 gatcggcttc ttcggtgtgg tctatggggc cctccgtggc ggaggctctg ctggcatcga
      841 tctgttctcg agctcggagg acgttgcggc catcttcgcc ctggaagtgg aggggcttcc
      901 gctctcggtc gacctcgagg atgtctggat acccaagtcc tggatcgtgg cggcccaggg
      961 tggggatgag tcgacagcct ccagcgacga cgagctggcc cgcggtgagg tcttccgtgt
     1021 ggccctgccg gtcttccagc aggtgtatcg gtacacggcc ggggatctcg acttgatcga
     1081 gctcctcagc accgaagcct ccggtcaggt ccatcactcc ccggaggaaa cgaatgcgct
     1141 gcatgcgtgg tcggcagaac agatcgagat cacacggcag gctttcccgt ccaagacggt
     1201 gaagctgcga taccgggagc agggcagtgc ctgagctggc ggtgaccatg ctcgatgtcg
     1261 gttggggcga ctgcatcctc gtcgatagcc gggacgacag cggggatcgc cgcttcggct
     1321 tgatcgactg caacgacttc gagcgccagc gcacggcgct cccattcgtc atgcgctact
     1381 tcgagagact ggggatcgac tggaaagccc ggtcacacaa cttcgaatgg gtgttgctca
     1441 cccacggaca cgccgaccat gcccggggac tgaaggagat gatgagcacc ttcggaactc
     1501 ggcatttttg gtatcccaag tcggtcgctt ccaccaccta cggtgttctt ctcgacttcg
     1561 ccaaccgatc gagtcgggtg ctgcaccatc aggcggtcga ccagacgaag atcctcgatg
     1621 ctcccgaggt gacttttcat gcggatctcc gcatcctctg gcccaatttc gaccagatcg
     1681 acacccggaa cgagaacaac aattcagtgg tcctggcggt cacactcggc cagtcgacca
     1741 tcgtcctcac cggggacgcc gaagccgaga attggcccgc gatcgtcccg cgcctcccgg
     1801 ccaccaccac ctatcttcag gtcccccatc acggtggtcg aaacggggtc ttcgatccca
     1861 acgagtccac tccctggctc gacggcatca gctaccgaaa gacacgactg gtgatgagca
     1921 gtcacatcgt gccccatggc catccgcatc cggatgtcgt cagcgtcctc gccgcgcgac
     1981 agttcgtcag ctaccgcacc gaccggaact acgacatcac cctcagcac
//
LOCUS       DATITW010000214         1543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_771460, whole genome shotgun sequence.
ACCESSION   DATITW010000214 DATITW010000000
VERSION     DATITW010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771460"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VLS86_02985"
     CDS             <1..496
                     /locus_tag="VLS86_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008095599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSO49487.1"
                     /translation="HCPTSNMRLASGLAPVSRYLREGVPVGLGVDGSSSNDSSHLMAE
                     TRQALLLNRLGVSPGVGHGEQMSARQALELATVGGARVLGRDDIGRLVPGMAADLIAF
                     DLNRVEYAGAIHDPLAAVVMCSPVTVDRSWVGGRRVVENGRMVGVEVSELVGEHNRLA
                     RALV"
     gene            540..1328
                     /locus_tag="VLS86_02990"
     CDS             540..1328
                     /locus_tag="VLS86_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HSO49488.1"
                     /translation="MVHPTSMEEALAGLSEGVVPLAGGTDLMVEVNFGHAHPERVTGL
                     RRVPELKEWEASRIGAGVTWRRIEQDGPNALAQAARTVGSPQIRNAGTIGGNVGTASP
                     AGDGHPFLVAVDASIELVSAAETRVLTWSEFFLGVKKTARRPDELIAAVHLPEVLPER
                     HEFGKIGVRNAMVIATVNCVVTRDDSGRVTVGLGSVAPTPIRVRGAEEMISAVAKPTD
                     ADLAEFQRLVSAEVSPITDHRSTEEYRRYGAGVLARRLLERCLR"
     gene            1325..>1543
                     /locus_tag="VLS86_02995"
     CDS             1325..>1543
                     /locus_tag="VLS86_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006143313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSO49489.1"
                     /translation="MIVGFTVNGQQVTARVTDPGSLLDVLRDDLGLPGSKSACEQGEC
                     GSCSVILDGELVCSCLVMAADVEGSSVTT"
BASE COUNT          231 a          496 c          551 g          265 t
ORIGIN      
        1 ccactgcccg acgtcgaaca tgcgtctggc atcgggcctg gctccggtct ccaggtatct
       61 gcgggaggga gtcccggtgg gcctgggcgt ggacgggtcg tcctccaacg actcctcgca
      121 tctcatggcg gagacccgtc aggcgctctt gctcaaccgg cttggggttt ctcccggcgt
      181 cggccacggg gagcagatgt cggcgcgtca ggcactggag ttggccactg tgggcggggc
      241 gcgggtgctc ggtcgtgatg acatcgggag actcgtcccg gggatggctg cggatctgat
      301 cgccttcgac ctgaaccggg tcgagtacgc cggggccatc cacgacccgc tggcggcggt
      361 ggtgatgtgc agcccggtca ccgtcgaccg ttcctgggta ggtgggcgtc gggtggtcga
      421 gaacggccgg atggtgggag tcgaggtctc agaactggtc ggggagcaca atcgactggc
      481 cagggccctg gtgtgacccc gaccggggct gcctcaacta acttcatcgg ccagtgcaga
      541 tggttcaccc gacctcgatg gaagaggcgc ttgccgggct ctccgagggc gtcgtccccc
      601 tggccggcgg caccgacctc atggtcgagg tcaacttcgg tcacgcacat cccgagcggg
      661 tgaccggtct tcgccgcgtg cccgagttga aggaatggga ggcatcccga atcggcgccg
      721 gagtcacctg gcgccggatc gagcaagacg ggcccaacgc gctggcgcag gctgcgcgca
      781 ccgtcgggtc gcctcagatc agaaacgccg ggaccattgg cggcaatgtc ggcaccgcca
      841 gccccgcggg cgacggacat ccgttcctgg tggcggtgga cgcctcgatc gagctcgtct
      901 ccgccgcgga gaccagggtc ctgacctgga gcgagttctt cctcggcgtc aagaagacag
      961 cccggaggcc ggacgagctg atcgccgcgg tgcaccttcc cgaggtgctc ccggaacggc
     1021 atgagttcgg caagatcggg gtccgcaacg cgatggtgat cgcaaccgtc aactgtgtcg
     1081 tcaccaggga cgacagcgga cgggtcaccg tcggtctcgg ctcggtggcc ccaactccga
     1141 tccgcgtccg cggcgccgag gagatgatct ccgcagtcgc caagcccacc gatgccgatc
     1201 tcgccgagtt tcagcgtctc gtctccgcgg aggtcagccc gatcaccgat catcgttcca
     1261 ccgaggaata ccggcgttac ggggccgggg tcctcgctcg ccgtcttctc gagaggtgtc
     1321 tccggtgatc gtcggcttca cggtcaacgg gcagcaggtc acggcccgag tgaccgatcc
     1381 cgggagcctg ctcgacgtgc tcagggacga cctggggctc cccggctcga agagtgcctg
     1441 tgagcagggc gaatgtgggt cgtgctcggt gatcctcgac ggtgagctgg tgtgctcctg
     1501 tctggtgatg gccgccgacg tggaagggtc ttcggtgacc acc
//
LOCUS       DATITW010000215         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_330805, whole genome shotgun sequence.
ACCESSION   DATITW010000215 DATITW010000000
VERSION     DATITW010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330805"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            495..1556
                     /locus_tag="VLS86_03000"
     CDS             495..1556
                     /locus_tag="VLS86_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSO49490.1"
                     /translation="MDWGSLTEDQLDRIIAQSEQEIAGWRVVEMAAIREKRSRQSHHA
                     DGYRSIVDWVAARADVSQQTSRSLCWTASRLEDAPEIVAQLESNEITFDRAEQLARLP
                     EGKREGHESYDISQLRRLVAHYRRLTPRREKRTGSFLNFQPSLDETTTNIWGELAGLD
                     ARMVEKAVDQRADEVLGSEVPLSVAERRALALVAICQDSLYNTESPEASPPVEVVVAV
                     DARTAAADGGETGVAVLAGPRIGPRALHEVFCNGIVQVVGLAEDGTPLSLGRRSRNVP
                     RRLRRFVLDRDMGCTVEGCSSRYRLEVHHTTPWSNGGRTDVDDLVALCWFHHHVAVHR
                     EGLRIVRVGRSRVRLKRPR"
     gene            1619..>2185
                     /locus_tag="VLS86_03005"
     CDS             1619..>2185
                     /locus_tag="VLS86_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HSO49491.1"
                     /translation="MDDLRHVLKLSADLIADYREALPSLRVGPTVGRAQVRESLGTLP
                     DHGESLETVLEELVSAARPGLMSSAGPRYFGFVVGGSLDAALVADLVATGWDQVAFNE
                     ATSPAALAFEDVAGMWLKDLLHIPRSASVGFVTGAQAANTVGLAAGRWRVLHDQGWDV
                     GRDGLNGAPPVRVVAGAERHATIDRTLRL"
BASE COUNT          394 a          696 c          707 g          388 t
ORIGIN      
        1 tcagtcgcgg gacttccggt gatggtggcg acttcatcgg atcgatcatt cgccgcccag
       61 ggcgcttgta atgccgacag cgatcatgac tgaacccacg accatgagta tccagcctcc
      121 tgccgccagc caccatgaga ccacccgacg catcgagctg tccgttccta cgtcgtattt
      181 cggatcggga ggaaggaacc aagtcgccca gctatggcgg gttcgaacgg caagcaatcc
      241 tgcagcgaag agagcgagtc caaatagcgc cgtcatacac gttcagtccc cgatcgatct
      301 cggtgaccaa gttttccagc ctacggagtc gacctctcac acgcgccact cagcgatccg
      361 gacggcaacg ggctggttgc ttcaggaatc aggggctgcg gcgtccatgg agactccgga
      421 aattcacgag aatctcttca aacaacgggt gattgggcac aaatttgtca caccccctcg
      481 gtacgttcat cactatggac tgggggagtc tcaccgagga tcagttggat cggatcatcg
      541 ctcagagtga gcaggagatc gccggctgga gggtggtgga gatggcagcg atccgggaga
      601 agagatcccg gcagtcgcat cacgccgatg ggtaccggtc gatcgtggac tgggtggcgg
      661 cacgagcaga cgtctcgcag cagacctctc gcagtctctg ctggaccgcg agccggttgg
      721 aggatgcacc cgagatcgtc gcccagctcg aatcaaacga gatcaccttc gaccgggccg
      781 aacaactcgc aagacttccc gagggcaagc gtgaaggtca cgagtcgtac gacatctccc
      841 agctccggcg tttggtggcc cattaccgca gactgacccc gcgccgcgag aagaggaccg
      901 gcagtttcct caacttccag ccgtccctcg acgagaccac caccaacatc tggggtgagc
      961 ttgccgggct cgatgcgcga atggtggaga aggccgtcga ccaacgcgcc gacgaggttc
     1021 tcggctcaga ggtcccgttg agtgtcgccg agagacgtgc cctcgccctg gtcgcgatct
     1081 gtcaggactc gctctacaac acggagtctc ccgaggcctc gccaccggtc gaggtggtgg
     1141 tggcagtcga tgcccgcacc gccgctgccg acggtggtga gaccggggtg gcggtgctag
     1201 ctgggccacg tatcggtccc cgagcccttc atgaggtctt ctgcaacgga atcgtccagg
     1261 tggtggggct agccgaggac ggcaccccgc tcagcctggg acggagatcg cgcaacgtgc
     1321 cacgcaggct gcgacgcttc gtgctggacc gggacatggg ttgcacggtc gagggctgct
     1381 cctcacggta ccggctcgag gtccaccaca ccacaccctg gtccaacggg ggcagaaccg
     1441 atgtcgacga cctggtcgcg ctctgctggt tccatcatca cgtggcggtc caccgcgagg
     1501 gtctgcgcat cgtgagggtt ggccggtcgc gggtcaggct gaaacgaccc agataggggt
     1561 gggcttcccc accaccccgc ctgtccccgc ctttccgctc accgttatct tcaggtccat
     1621 ggatgatctt cgccacgtct tgaagctgag cgccgacctg atcgccgact atcgggaagc
     1681 acttccgtcg ctccgggtcg gcccgaccgt aggcagagcg caagtgcggg agagtctcgg
     1741 cacgctgccc gaccacggcg agagtctcga gacggtgctc gaggagttgg tgtcggcggc
     1801 gcgccctggt ctcatgtcct ccgccgggcc ccgctacttc gggttcgtcg tgggtggctc
     1861 gctggacgca gctctggtcg ccgatctggt tgccaccgga tgggaccagg tggcgttcaa
     1921 cgaggcaaca tcaccggctg cgctcgcctt cgaagatgtc gccggaatgt ggctcaaaga
     1981 cctgctccat atcccgcggt ctgcctcggt gggattcgtc accggtgctc aggcggcaaa
     2041 caccgtcggt ctggccgcag gccgatggcg cgtcctccac gatcagggat gggatgtggg
     2101 gcgtgacggg ctgaacggcg ctccaccggt acgggtggtg gccggggcgg agagacacgc
     2161 caccatcgac cgcacccttc gtctc
//
LOCUS       DATITW010000216         1632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_50585, whole genome shotgun sequence.
ACCESSION   DATITW010000216 DATITW010000000
VERSION     DATITW010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50585"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VLS86_03010"
     CDS             complement(<1..197)
                     /locus_tag="VLS86_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HSO49492.1"
                     /translation="MFRVGRPQLRPAQRRLRRAVTVADLRSIARRRLPGGVFDYIDGG
                     AEDELTLAANSNAYRRVTFRPR"
     gene            complement(242..1024)
                     /locus_tag="VLS86_03015"
     CDS             complement(242..1024)
                     /locus_tag="VLS86_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSO49493.1"
                     /translation="MRIARYRQGTRHGVGIIEGGHIVPAGDDLLAPIPDGEAIPLAEI
                     DFLPPVLTTAKIVCVGLNYADHAAEGGVALPDQPLIFSKLPNSLTGHRSPIVLSPLSE
                     QVDFEAELGVVVGRRARGVSKEEALAYVFGYTCVNDVSARDLQHGDQQWTRGKSLDTF
                     CPVGPWVVTSDQIPDPQALGIRCLINGEVMQDSHTSNMVFGVDEIVSFISQGITLEPG
                     DLIATGTPGGVGFLQRPPRYLATGDSVRIEIDGIGYLENSVV"
     gene            complement(1021..>1632)
                     /locus_tag="VLS86_03020"
     CDS             complement(1021..>1632)
                     /locus_tag="VLS86_03020"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HSO49494.1"
                     /translation="LVSVGVLDVRRGDGTYVTSLRPEQLLEGIGAAAELMAEGFSLEL
                     VQVRRILEPAATAMAALRIDQKTLSLLESHLQRMGASETEEELVQIDSDFHAVVAGAS
                     GNSTLASMLNGVSTRTARTRTWRGVIEGEAKARTIAQHANILHALTRRDPALAEAAAL
                     VHVATTEDWLRRLTGAAADIEESAGANTRPRLKGDADDEAGPS"
BASE COUNT          302 a          513 c          511 g          306 t
ORIGIN      
        1 cggggccgga atgtcacgcg ccgataggca ttgctgttgg cggcgagagt cagctcgtct
       61 tcggccccac cgtcgatgta gtcgaagacg ccgccgggga gccggcgcct agcgatagat
      121 ctcaggtcgg cgaccgtaac cgcccgacgg agccggcgtt gagcggggcg cagttggggc
      181 cggccgaccc gaaacatcct caaggtcgac gcgagactgt tgcctttgcg cgatcccggt
      241 gtcatacgac agagttctcg aggtaaccga tcccgtctat ctcgatcctc acgctgtcgc
      301 ctgtcgccag atagcgggga ggtctctgca ggaagccgac acccccaggg gtaccggttg
      361 cgatcagatc gccgggctcg agagttatgc cttgggagat gaagctgacg atctcgtcga
      421 caccgaaaac catattggaa gtgtgtgaat cctgcatgac ctcgccgttg atcaaacagc
      481 gtattccgag tgcctgcgga tccgggatct gatcggaagt cacgacccat gggcccacgg
      541 ggcagaacgt gtccagcgat ttgccacggg tccactgctg gtcgccatgc tggaggtccc
      601 gggccgaaac atcgttgaca caggtgtatc caaacacata cgccagcgcc tcctctttgg
      661 aaacccccct cgcacgacgc ccgacgacga ctccaagttc ggcttcgaag tcgacctgtt
      721 cgctgagagg ggagaggacg atcggactcc tgtgtccggt gaggctattg ggcagctttg
      781 agaagatcaa cggttgatcg ggcagagcaa cgccgccttc agcagcgtga tcggcatagt
      841 tgagacccac gcacacgatc ttggcggttg tcagcaccgg cggcaagaaa tcgatctcgg
      901 caagcgggat cgcttctccg tcgggaatgg gcgcgaggag gtcgtcaccg gcgggaacga
      961 tgtggccccc ctcgatgatc ccgacgccgt gccgtgttcc ttgccgatat ctggcgatcc
     1021 tcacgatggt cctgcttcat cgtccgcatc gcctttcagg cgcggccggg tgttggcacc
     1081 agcggattct tcgatgtcgg cggccgcccc ggtcaatcgc cggagccagt cctcggtcgt
     1141 tgcgacgtgg accagcgctg cagcttcggc gagagcaggg tcacgccggg tcagagcgtg
     1201 caggatgttg gcatgctgag cgatggttcg agccttggcc tctccctcga tgacgccccg
     1261 ccatgttcga gtacgtgcag tcctggtcga cactccattc agcatcgatg ccaatgtcga
     1321 gttgcccgaa gcacccgcga ccaccgcatg gaagtcactg tcgatctgca ctaactcctc
     1381 ctccgtctcc gaggcgccca tccgttgaag gtgcgactcc aggagggaaa gggtcttctg
     1441 gtcgatccgc aatgcggcca tggcggtagc agccggttcg aggattcgac gcacctggac
     1501 cagttcaagc gaaaaccctt ccgccatcag ctcggcggcc gccccgatcc cttccaacag
     1561 ctgctcgggt cgcaagctgg tgacgtaggt gccgtcgcct cgccggacat cgaggacacc
     1621 aacgctcacg ag
//
LOCUS       DATITW010000217         1687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_771630, whole genome shotgun sequence.
ACCESSION   DATITW010000217 DATITW010000000
VERSION     DATITW010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771630"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..612
                     /locus_tag="VLS86_03025"
                     /pseudo
     CDS             13..612
                     /locus_tag="VLS86_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            609..1580
                     /locus_tag="VLS86_03030"
     CDS             609..1580
                     /locus_tag="VLS86_03030"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSO49495.1"
                     /translation="MKILVTGGAGYIGSHLVDRLLSRGDEVFAIDNLSTGKLANIQQH
                     LESDRFHLSNDTILNESLIDHLVSQVDLVYHLAALVGVKYVVADPLGAIKTNVTGTEV
                     VLGAAFKYWKKVVLASSSEIYGKSAKIPFHEQDDRVLGPTNVGRWSYSTSKAIDEHFA
                     FAYSAKGLSIAIVRYFNSYGPRLDEKGYGSVVANFVRQALKGEPITVHGDGLQSRCFT
                     YIDDTVDGTLLAGEVKAGEGLVFNIGNDAETTVHDLAVAIKRLTGSRSEIIRVPYKEY
                     YGKGFEDTRRRVPSIDRAKDVLGFTPKVKLEEGLMRTIAWCREHYSN"
BASE COUNT          339 a          505 c          519 g          324 t
ORIGIN      
        1 ctccgacgat cgttgggggt ctcgcccgag gtccctcttg tgggcatcat cgcccggctc
       61 gtaccgatca aagaccacag cacattcctg gaggcggcag ccgacctcca ccgatctcgg
      121 ccggatgtcc ggttcctgat cgtcggtgac ggcgaactca gaccgcacct ggagcaacag
      181 gctcacgcct tgggcctgag ccggtgcgtc actttcctcg ggtggcaacg cgagctggaa
      241 ccgatctagg cggacctgaa tctcgcggtc ctgtcgtccc ggaacgaggg cactccagtg
      301 tctctcatcg aggccatggc ggcaggcctt ccggtggtag ccaccagggt cgggggggtc
      361 tccgacctgg tcgaacacgg gaaaaccggc ctgcttgtcc cgcccaagga tcccgaggcc
      421 ttgagcggcg cgatgaaggc cctcctcggc gaccccgaca gggggcgcga gatgggccga
      481 ctggcgcggg agaccgtcta tccgacgtac agcgatgctg cgctcatcga tcgaatggac
      541 cacctctact cctccatgct ccatgccagc ctcccggctc ggcgatccgc gcatgagggg
      601 tcgaacagat gaagatcctc gtgaccggcg gcgctggata catcgggtct cacctggttg
      661 accgtctgct cagtcgtggc gatgaggtgt tcgccatcga taacctctcg accgggaagc
      721 tcgccaatat ccagcagcac ctcgaaagcg accgattcca cctttcgaac gacacgatac
      781 tgaacgagag cctcatcgac cacctggtct cgcaggtcga tctggtatat catctggctg
      841 ccctggtcgg cgtgaagtac gttgtggccg atccgctagg cgccatcaag accaatgtga
      901 ccgggaccga ggtggtgtta ggtgcggcgt ttaagtattg gaagaaggtg gtgttggcat
      961 caagctccga gatttatggc aaatcggcca agatcccatt ccacgagcag gacgatcggg
     1021 tcctgggtcc gacgaacgtg ggccgctggt cctactcgac ctcaaaggca atcgacgagc
     1081 attttgcctt cgcgtattcg gccaaagggc tctccatcgc tattgtccgc tatttcaact
     1141 cgtatgggcc gcgcctggat gaaaagggct acggcagcgt ggtggctaac ttcgtccgcc
     1201 aggccttgaa gggcgagccg atcacggttc acggcgacgg attgcaaagc cgctgcttca
     1261 cctacatcga tgacactgtg gatgggacgc tcctggccgg agaggtcaaa gcgggtgaag
     1321 gcctggtctt caatatcggg aatgatgcgg aaaccacggt ccatgacctg gcggttgcca
     1381 ttaaacgact gaccggttcc cgatctgaga tcatccgtgt cccgtacaag gaatactatg
     1441 ggaaggggtt cgaggacacg cggcggcgag ttccgtcgat cgaccgagcc aaagacgttc
     1501 tggggttcac cccaaaggta aagttggaag aggggctcat gcgcaccatc gcctggtgcc
     1561 gtgaacacta cagcaattga ccgaccttgc cggagaatcg acgcctcatc gaacattcac
     1621 tcggaaggtt gagaccaaac aagcaacggt ggccatcatc ggcctgggct atgttgggct
     1681 gccgatg
//
LOCUS       DATITW010000218         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_491041, whole genome shotgun sequence.
ACCESSION   DATITW010000218 DATITW010000000
VERSION     DATITW010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491041"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VLS86_03035"
     CDS             complement(<1..1076)
                     /locus_tag="VLS86_03035"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSO49496.1"
                     /translation="MNGTTIGLIAVAVVFGIDIILLSGLVLLKAVHRRRREDYARRRA
                     EYVSILSRHLAFRHHTDTFGPNAAEDDAFLDAVIDLRNIVSGTEIDTLAGIIDSVGVA
                     RRQEARLNHRFPLGRRLRAAVSLAEMGDESSASVLIDHLNDREPEIRIQCARGLGRMQ
                     YTAAIDPILERLALEDPWVRARFADTLVGFGTKATWPLVAYIRVNLGHDDNRGVIEAI
                     RVLGTIGDQEVGPSLAGVLRVASDPEVGIAAIEALGLVGGPLAIRPLKYTLRSPDWRL
                     RAKSATALGQIGDPSINPVIAESLEDDNWWVRRNSAAALTALPGGTDLLLGVIESGTS
                     FAADAAAEALADSGALAEARHRVDT"
     gene            complement(1134..1502)
                     /locus_tag="VLS86_03040"
     CDS             complement(1134..1502)
                     /locus_tag="VLS86_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSO49497.1"
                     /translation="MTEAKRVLIADDSETVTTLLATALELEGYEVDTAVNGVEAYEKG
                     ISGGFDLVILDQLMPGLLGLEVIDRWQQEGHLNRVIMLSGVDDDRTVVSSLDLGAVDF
                     IRKPFHLPELMARIRQRLPR"
     gene            1641..>2097
                     /locus_tag="VLS86_03045"
     CDS             1641..>2097
                     /locus_tag="VLS86_03045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HSO49498.1"
                     /translation="MSTIPDHEMSRLESLARPEAPLEDLVARRLAGEPLQYIEGSAAF
                     GPLDLVVDDRVLIPRPETEGLFELATRLVRAPEVIVDLCTGSGALGLALKSFFPSAAV
                     FATDISPDAIEVATSNRYRTGHQVYLAEGNLFDPLPTAILGTVDLIVANP"
BASE COUNT          351 a          689 c          686 g          371 t
ORIGIN      
        1 gtgtctactc gatgcctcgc ctcggcgagg gcgccggagt cggccagggc ctcggctgca
       61 gcatcggccg cgaacgatgt gccggactcg atgaccccca gcagcaggtc ggtgcccccg
      121 gggagcgcgg tgagcgctgc tgcgctgttg cgacggaccc accagttgtc gtcttcgaga
      181 ctctcggcga tcaccgggtt gatcgaaggg tcgccgatct ggcccagggc ggtggccgac
      241 tttgcccgga gccgccagtc gggcgagcgg agcgtgtact tgagcggtcg gatcgccaga
      301 ggcccaccga ccagtcccag ggcctcgatg gcagcaatcc ccacctccgg gtcactggca
      361 accctgagca ctcccgccag ggaggggcca acctcctggt cgccgatcgt acccagcacc
      421 cggattgcct cgatcacacc ccggttgtcg tcgtgaccga ggttgacccg gatgtaggcg
      481 acgaggggcc acgtggcctt ggtgccgaag ccgaccaggg tgtcggcgaa gcgggcccgc
      541 acccatggat cctcgagggc gagccgctcc aggatgggat cgatcgccgc ggtgtattgc
      601 atgcgaccca ggcctcgtgc gcattgaatc ctgatctccg gctcacggtc gttgagatga
      661 tcgatcagca ccgaggccga agactcgtca cccatctcgg ccaacgacac cgcagcccgc
      721 aatctgcggc ccagtgggaa gcgatggttg agccgggcct cctggcgccg ggcgacgccg
      781 acactgtcga tgatgccggc gagggtgtcg atctcggttc ccgagacgat gttccgcagg
      841 tcgatcacgg catcgaggaa ggcgtcgtcc tcggcggcgt tgggcccgaa cgtgtcagtg
      901 tggtgccgga aggcgagatg gcggctcagg atcgacacgt attcggcgcg gcgccgcgcg
      961 tagtcctccc tgcgacgccg gtgcacggct ttgagcagca ccagccccga aagcagaatg
     1021 atgtcgatgc cgaagacaac cgcgaccgcg atcaagccga tcgttgttcc gttcatgaga
     1081 tctctcagta ctcatcggtc actgcgatga cctcattgag gaatcgtccc gattcagcga
     1141 ggcaatcgtt gacgaatccg tgccatcaac tcggggagat ggaagggctt gcggatgaag
     1201 tcgacggccc cgagatcgag gctgctgacc acggtcctgt cgtcgtccac accggagagc
     1261 atgatgaccc gattcaggtg cccctcctgc tgccagcggt cgatgacctc caaaccgagc
     1321 agaccgggca tcagctggtc gaggatgacc aggtcgaacc ccccggatat ccccttctcg
     1381 taggcctcga caccgttcac cgcggtgtcg acctcgtaac cctccaattc gagggcggtc
     1441 gccaacagag tcgtcaccgt ctccgagtcg tcggcgatca gcactctttt ggcttcagtc
     1501 atcgagcgtc ctttgcctct cccgggcgca gttgcagccc tgatgatggc aggttcgacc
     1561 gtcgggatcg gtgctacacc tccacctggg gcgaagcggt agcttcaatt cgctattcgg
     1621 cgtgggagac ttggggcgcc atgtcgacga ttcctgatca tgagatgtcg aggctcgagt
     1681 cgctggcgag accggaagca ccgctcgagg acctggttgc gcggcgtctc gccggtgagc
     1741 cgttgcagta catcgagggc tccgcggcgt tcggccccct ggatctggtg gtcgacgaca
     1801 gggtgctcat cccccggccc gagaccgagg gcctgttcga gctggcaact cgcctggtga
     1861 gggcgccgga agtgatagtg gacctctgca ccggctcggg tgccctcggt ctcgccctca
     1921 agagcttctt cccatccgca gcggtgtttg ccaccgacat ctccccggac gcgatcgagg
     1981 tggcgacttc caaccgctac cgcaccggcc accaggtgta tctggcggag ggcaacctgt
     2041 tcgacccgct gccgacggcg atcctgggga ccgtcgacct gatcgtggcc aacccgc
//
LOCUS       DATITW010000219         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_90711, whole genome shotgun sequence.
ACCESSION   DATITW010000219 DATITW010000000
VERSION     DATITW010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90711"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1899)
                     /locus_tag="VLS86_03050"
     CDS             complement(<1..1899)
                     /locus_tag="VLS86_03050"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HSO49499.1"
                     /translation="MDNRLGRIAGVAAFALMLTRLGRLLDSAGEAPEWQMIMIASAFL
                     GGVIFWLLAQTVSNRRIAIGVFVVAATALFLRISVSPTLVYGFIPTAGTLDALGREVT
                     QALDLIRFGVSPVFPTPGIIAILAIVMWGIGALYVWGASRGPSIAMILPSFGLYLQFA
                     VMDRLPAGMGWMAAAAAVIALGLTAIGIERRQDTGRMRDLDGRPLARRAGGVALVVAL
                     VIAIGSVAMAMNASSLVPQNGNLSWRYGGGYGPGFGGVAFDRLADLQQSIIRRSNSVM
                     FLATLDSNAPPANEIYWRMESLDIFDGTAWRPSASRADFYEPGVGGGDPEYAYRGTTR
                     DITQRIRIVDLRSQVVPTVGVGQFFRSDSVNVSAFQITPDGSAIFQPELEEGDEYEVQ
                     SVLAMNMDDLGALATRSDGTLSPLFANASEAEASEIEPGLPPGDVTLPANIERFVDLP
                     EEVPSGILQVAREQTARATTPFEAAWLLQHWFRDSGEFEYSTDVSTGHASLDLEDWLT
                     EPTSINYRIGYCEQFAAAMAVLGRSVGIPSRVVWGFTPGRVEQQSDGSEVVVVRDNNA
                     HAWVEMWMDGFGWVRFDPTPRGDGALPESITAEFDPEPFLPPPGDPDAAIVDRPSFLD
                     ESLADFDRV"
     gene            complement(1875..>2060)
                     /locus_tag="VLS86_03055"
     CDS             complement(1875..>2060)
                     /locus_tag="VLS86_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49500.1"
                     /translation="QQLLSRDYPTTVLMTASESTSQTLVSFHRLGVGTVSVAPDQPWA
                     PAWQASLRNSWTTASVG"
BASE COUNT          371 a          681 c          687 g          321 t
ORIGIN      
        1 gacccgatcg aagtcggcca gcgactcatc gaggaagctc gggcgatcga cgatcgccgc
       61 gtcggggtcg cccggcggag ggaggaacgg ctccgggtcg aactcggcgg tgatgctctc
      121 ggggagcgca ccgtcgcctc gtggtgtggg atcgaacctc acccatccga acccgtccat
      181 ccacatctcg acccaggcgt gggcgttgtt gtcccgcacc accaccacct cgctgccatc
      241 ggactgctgt tcgacccggc ccggggtgaa cccccatacc acccggctcg ggatgcccac
      301 cgaacggccc agcaccgcca tggcagccgc aaactgctcg cagtaaccaa tccggtagtt
      361 gatactggtc ggctcggtca gccagtcctc gaggtcgagg ctggcatgac cggtagagac
      421 gtcggtcgag tactcgaact ccccgctgtc gcggaaccaa tgctggagga gccaggcggc
      481 ctcgaagggt gtggttgccc tggcggtctg ttccctcgcc acctggagga tcccggaagg
      541 cacttcctcc ggcaagtcga cgaagcgctc gatgttggcg gggagggtca cgtcgccggg
      601 cgggaggccg ggctcgatct ccgaggcctc ggcctcggag gcgttggcga agagcgggct
      661 gagggtcccg tcgctcctgg tggcgagggc gcccaggtcg tccatgttca tggccagaac
      721 cgattgcacc tcgtactcgt cgccctcctc cagctctggt tggaagatgg ccgacccatc
      781 gggtgttatc tggaacgcgc tgacattgac cgagtcggat ctgaagaact ggccgacacc
      841 caccgttggc accacctggg accggagatc gacgatgcga atcctctgcg tgatgtcccg
      901 ggtggttccg cgataggcat actccgggtc accgcctccc actcccggct cgtagaagtc
      961 ggcccggctc gccgaggggc gccaggcggt gccgtcgaag atgtcgaggc tctccattct
     1021 ccaatagatc tcgttggcgg gtggggcatt ggagtcgagg gtcgccagga acatcaccga
     1081 gttcgagcga cggatgatgc tctgttgcag gtcggcgaga cggtcgaagg ccactccgcc
     1141 aaacccgggg ccgtagccac cgccgtatcg ccagctgaga ttgccgttct gcggcaccaa
     1201 cgatgaggca ttcattgcca tcgccaccga accgatggca atgaccaggg ccaccacgag
     1261 cgccacgcca cccgctctgc gggccaatgg gcgaccatcg agatcccgca tccgaccggt
     1321 gtcctggcga cgttcgatac cgatcgcggt gagccccagg gcgatcaccg ccgccgcggc
     1381 cgccatccag cccatccctg cgggcagccg gtccatgacc gcgaactgga ggtagaggcc
     1441 aaacgagggc aggatcatgg cgatcgacgg gccccgggag gcaccccaga cgtatagcgc
     1501 cccaattccc cacatgacga tcgccaggat ggcgattatc cccggggtgg gaaagaccgg
     1561 ggagaccccg aatcggatca ggtccaatgc ctgggtcacc tcgcgtccca gggcgtcgag
     1621 ggtcccggcc gtcgggatga agccgtacac cagggtcggt gacaccgaga tccggaggaa
     1681 gagcgcggtg gcggccacaa cgaacacgcc aattgcgatt ctccggttgc tcacggtctg
     1741 agcgagcagc cagaagatga ccccacccag gaaggcggac gcgatcatga tcatctgcca
     1801 ttccggcgcc tcgccggcgg agtcgagcag gcgccccagc cgcgtcagca tcaaggcgaa
     1861 ggcggcgaca ccggctatcc gaccgaggcg gttgtccatg agttcctcag agatgcctgc
     1921 catgccgggg cccacggctg gtcgggtgcg accgagaccg tgcccacccc gagccggtgg
     1981 aaggagacga gtgtctgcga tgtcgactcg gacgcggtca tcagcaccgt tgtcgggtag
     2041 tcacgggaca ggagctgttg
//
LOCUS       DATITW010000220         3348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_451183, whole genome shotgun sequence.
ACCESSION   DATITW010000220 DATITW010000000
VERSION     DATITW010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451183"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VLS86_03060"
     CDS             complement(<1..698)
                     /locus_tag="VLS86_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSO49501.1"
                     /translation="MNTPQPQSLGNGIHLIPAPLPFKSPAWVNTYAVEADGGFLLLDC
                     GTDWEPGRDAIRDGFAQLGLEESAVHTLVVSHLHLDHVGMSSRMVRELGCRFVMHERA
                     AKLVDRYNDTPGYVERLLRIGHIHGVPPPLLATVSDDLAIRPDYMPLIDPPDHTVQDG
                     ERIEIGAGRWLEVVHTPGHDSAHICLRDSQTGILFSGDHILPRISPVIMWDEDLGDPL
                     GDYLASLQRLVGME"
     gene            complement(695..919)
                     /locus_tag="VLS86_03065"
     CDS             complement(695..919)
                     /locus_tag="VLS86_03065"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HSO49502.1"
                     /translation="MSSITKTIRLSGQAGDSIEDAVRTVLARAAATISDIQSFKIVEV
                     GGKVDDSGAPTLFTVTLDVTFGIKDPVEHG"
     gene            complement(944..1813)
                     /locus_tag="VLS86_03070"
     CDS             complement(944..1813)
                     /locus_tag="VLS86_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO49503.1"
                     /translation="MHDTSVPVDHGRISVWFRPAIPGKPTVLLIHGLTGTSRWWAPVI
                     ARLPEEVGVIAPDLRGRGQSWEHPGPFDLSSMAPDISACLNHFEIPEATIAGYSMGAW
                     LVTVYASTRHRRVKGIVLVDGGLRVEVDTDELPDDALDRVIGASLRRFEMSFETEDAY
                     LSFWRSHPALVGRWGAHLDMIFAYDLHQVRDGSFQNRANYQAVIQGANDFMFDEQTIK
                     AVNELTVPTRLLIVDHGTADEPGGFLSGDAAAETTSRNPNVTVERLRDLNHYTVLLGS
                     GASHVASAIVRLL"
     gene            1912..2790
                     /locus_tag="VLS86_03075"
     CDS             1912..2790
                     /locus_tag="VLS86_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HSO49504.1"
                     /translation="MRQAFLGAFVVLVVSCSSPGEDALSSTVTTSPVTTVVTTTTTTT
                     TPTTTTAPTTTTTNPHARPDWLGTRLLPLRPDEFGEVQPTPPELQDRQLETLDLLPPP
                     ADETFLATIDPIPPDVLARSSWVAECPVTLDELSYLTITHYGFDGRFHTGEMIVNAAF
                     ADEVVGIFRKLHEARFPIEQMRVITKEEIDAHPTGDWNDTTSFVCRPAVGSSSWSQHA
                     FGTGIDINPFHNPYLKGDLVLPELASAYTDREDVRPGMIVPGDVVTEAFAEIGWGWGG
                     NWSSLKDWMHFSRSGR"
     gene            2856..>3348
                     /locus_tag="VLS86_03080"
     CDS             2856..>3348
                     /locus_tag="VLS86_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HSO49505.1"
                     /translation="MSGYAELHCHTNFSFLDGASHPEDLVERAIDLGYEALAVTDHDG
                     FYGVSRFWQAARESGLPAIYGAEIGIEDDDPVGPPDPVASAQEWGARRYARSKGAQGR
                     LRRGRSVRSHGAKPTNLPESNHLVLLAGSPDGYRSLSNLVTTAQMRGEKNHPLYTWDD
                     LAQA"
BASE COUNT          578 a         1107 c         1081 g          582 t
ORIGIN      
        1 atctccatcc ccaccaggcg ctgtagcgat gccaggtagt cgcccagggg gtctccgaga
       61 tcctcgtccc acatgatgac cggcgagatc cggggcagga tgtggtcgcc ggagaacagg
      121 atccccgtct gcgaatcacg caggcagatg tgggccgagt cgtgaccggg ggtgtggacc
      181 acctccagcc accgcccggc accgatctcg attcgctcgc cgtcttgcac cgtgtgatct
      241 ggcgggtcga tcaacggcat gtagtcgggt cggatcgcca ggtcgtcgga gacggtggcg
      301 aggaggggcg gggggacccc gtggatgtgg ccgatccgaa gcaggcgctc gacgtatccc
      361 ggagtgtcgt tgtagcgatc gaccagcttg gccgctcgct cgtgcatgac gaaccgacag
      421 ccaagctctc tcaccattcg cgaagacatc ccgacatggt cgaggtggag atgggagacg
      481 accagggtgt ggaccgcgct ctcttcgagg ccgagctgtg cgaagccgtc ccggatcgca
      541 tctcgccccg gctcccagtc ggtgccgcag tcgagcaaca ggaagccgcc atcggcctcg
      601 actgcatagg tgttgaccca ggctgggctc ttgaacggaa gtggcgccgg gatgagatgg
      661 atcccgttcc ccaacgactg gggttggggg gtgttcatcc gtgctcgact gggtccttga
      721 tcccgaaggt tacgtccagt gtcaccgtga acagagtggg cgccccggag tcatcgacct
      781 tgcccccgac ctcgacgatc ttgaagctct ggatgtccga gatcgtggcc gcggcccggg
      841 ccagcacggt gcgcaccgcg tcttcgatcg agtcgccggc ctgaccggag agccggatcg
      901 tcttggtgat ggatgacatc gatttctccc gaggtgacgg tagctacagc aagcggacga
      961 tggccgaggc aacgtgggat gcgccgctgc cgagcaggac cgtgtagtga ttgaggtcac
     1021 gcagccgctc caccgtgaca ttggggttcc tcgacgtagt ctccgccgca gcgtcgccgg
     1081 agaggaagcc cccgggctcg tccgcagtcc catggtcgac gatcagcaac cgggtgggga
     1141 cggtcagctc gttcaccgcc ttgatcgtct gctcgtcgaa catgaagtcg ttggcgccct
     1201 ggatgaccgc ctggtagttg gcccggttct ggaagctccc gtcgcggacc tggtgcaggt
     1261 cataggcgaa gatcatgtcg agatgggccc cccatcgacc gaccagcgcc gggtggctac
     1321 gccagaagct cagatacgca tcctcggtct cgaagctcat ctcgaatctc ctcagcgaag
     1381 cgccgatcac ccggtccagg gcatcgtccg gaagctcgtc ggtgtcgacc tcgacacgga
     1441 gcccgccatc gacgagcacg atccccttga cccgtcgatg acgggtgctg gcgtacaccg
     1501 tgaccagcca agcccccatc gagtaaccgg cgatggtggc ctctgggatc tcgaagtggt
     1561 tgaggcaggc cgagatgtcc ggcgccatcg acgacaggtc aaacgggccg gggtgctccc
     1621 aactctgacc acgcccccgg agatcgggcg cgatcacccc cacctcctcc gggagccgcg
     1681 cgatgacggg ggcccaccat ctggaggtgc cggtcaggcc atggatgagc aggacagtcg
     1741 gtttgccggg aatcgccggc cggaaccaca ccgagatcct cccatggtcc acgggcaccg
     1801 aggtgtcgtg caccccccaa acctatcgga tctcgcctgg taatagtcgg tcgcgagccc
     1861 gggccggctg ccgtcccggg agagatgatg gcgatggtta gggtctgttt ggtgcgacag
     1921 gcgtttctcg gcgcgttcgt ggtgctggtg gtcagctgtt cgtcacccgg tgaggatgcc
     1981 ctctccagca ccgtgaccac ttctccggtc accactgtcg tgaccaccac gacgacgacc
     2041 acgaccccga ccaccaccac ggcgccgacc accacgacca ccaatcccca cgcccgcccc
     2101 gactggcttg gcacgagact gctgccactg cgtcccgacg agttcgggga ggtgcagccg
     2161 accccacctg agcttcagga tcggcaactc gagaccctgg acctgctgcc cccacccgcc
     2221 gacgagactt tcctggcgac tatcgacccg attccccccg atgtcctggc ccgttccagc
     2281 tgggtggcgg agtgcccggt cactctcgac gagctgtcct acctgacgat cacccactac
     2341 gggttcgacg gccgcttcca caccggggag atgatcgtca atgcggcgtt tgccgacgag
     2401 gtggtcggca tctttcgcaa gctgcacgag gcccggttcc ctatcgagca gatgagggtg
     2461 atcaccaagg aggagatcga tgcccatccc accggggact ggaacgacac cacctctttc
     2521 gtctgccgac ccgcggtcgg ctcatcgtcc tggtcgcagc acgcctttgg caccgggatc
     2581 gacatcaacc ccttccacaa tccctacctc aagggggatc tggtgctgcc cgagctggca
     2641 tccgcctata ccgatcggga agacgtgcgc ccggggatga tcgtgcccgg ggacgtcgtg
     2701 acagaggcct tcgccgagat cggctggggg tggggaggca attggagctc gctcaaggac
     2761 tggatgcact tcagccgcag cgggcgatga cggctcccca ctcgtgatgg cggggccctc
     2821 cgcgcctcga acatatgttc gatatagtgc gatgagtgag cgggtacgcg gaacttcact
     2881 gtcacaccaa cttctccttt ctcgatggtg cctctcaccc cgaggatctg gtcgagcgag
     2941 ccatcgatct cggctatgag gcgttggcgg tcaccgatca cgacggtttc tatggcgtct
     3001 cccggttctg gcaggcagcg agggaatccg ggctccctgc gatctatgga gccgagatcg
     3061 ggatcgagga cgacgacccg gtcggccccc ccgacccggt ggcatcggcc caggaatggg
     3121 gcgcccgtcg ctatgcgcgc agcaaggggg cccaaggccg gctccgccgt ggtcggagcg
     3181 tgcgctctca tggtgccaag ccgaccaatc tgcccgagtc gaatcatctg gtcctgctcg
     3241 ccgggtcgcc cgacgggtat cggagtctct ccaacctggt gaccaccgcc cagatgcggg
     3301 gggagaagaa ccatccgctg tacacctggg acgacctcgc ccaagcct
//
LOCUS       DATITW010000221         1589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_291053, whole genome shotgun sequence.
ACCESSION   DATITW010000221 DATITW010000000
VERSION     DATITW010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291053"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1040
                     /locus_tag="VLS86_03085"
     CDS             63..1040
                     /locus_tag="VLS86_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HSO49506.1"
                     /translation="MTTTTEDQVIFETAAEHLARAVPVASATDTVGAIREGLGGRRFE
                     TAAAVAVCDEGRLVGLVRIEDLLAAPEGASVAEVMDGEPPVVGPGMHQERAAWQAVQH
                     GESTIAVVDEQRMFLGLIPPSRMLQVLLWEHEEDLARLGGFVHDAEKAREASEEAVAR
                     RYWHRLPWLLLGLAGALVSAVLIGAYEQELEANVLLALFIPGIVYMADAVGTQTETLI
                     IRGLSVGVKVRQVVKRELLTGALVGVTVAAAFYPLALWRWSDFDVAVTAALALLAACS
                     TATVVAMALPYLLDRLGKDPAYGSGPLATVIQDLISILVYFAIASAIMG"
     gene            1086..>1589
                     /locus_tag="VLS86_03090"
     CDS             1086..>1589
                     /locus_tag="VLS86_03090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007237110.1"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HSO49507.1"
                     /translation="MRAEASILHADLDAFYASVEQRDDPRLRSRPVIVGSGVVLAASY
                     EARAFGVRTAMGGGVARRLCPGAIVVQPRISAYSEASKAVFDLFRDTTPLVEGLSIDE
                     AFLDVGGLKRVSGSPIEIARRLRRRVFDEVGLPITVGIARTKFLAKVASGVAKPNGLL
                     VVAAGTEL"
BASE COUNT          257 a          480 c          558 g          294 t
ORIGIN      
        1 cttgggtcgg cgcgggactc gcgaacccgc gaagctctgc gggccgacca aggagatact
       61 cgatgacgac caccaccgaa gatcaggtga tcttcgaaac cgcagccgag catttggcga
      121 gagccgtgcc ggtggcgtca gcaacggaca cagtcggggc catccgcgaa gggctggggg
      181 gccggcggtt cgagaccgcg gctgcggtgg ccgtctgtga cgaggggcga ctggtcggtt
      241 tggtccggat cgaggacctg ctcgccgccc ctgaaggggc gtccgttgcc gaggtgatgg
      301 atggagaacc tccggtggtt ggccctggga tgcatcagga gcgggccgcc tggcaggcag
      361 ttcagcatgg cgagtcgaca attgcggtgg tcgatgagca acggatgttc ctgggcttga
      421 tcccgccatc gcgaatgctg caagtcctcc tctgggagca cgaagaggac ctggcgcggc
      481 tcgggggttt cgtccacgac gcggagaagg cccgagaggc gagtgaggag gcagtcgctc
      541 gccgttattg gcatcgtctc ccttggctgt tgctcggtct cgccggagca ctggtttcag
      601 cggtcctgat cggggcctac gaacaggagt tggaggccaa tgtcttgctg gcgctgttca
      661 ttcctgggat cgtctacatg gcggacgcgg tgggcaccca gaccgaaacc ctgatcattc
      721 gcggtctgtc ggttggggtg aaggtccgcc aggtcgtcaa acgcgaacta ctcaccgggg
      781 ccctggtcgg ggtcacggtc gccgccgcct tctacccgct ggctttgtgg cgatggagtg
      841 acttcgacgt ggcggtgacc gcggctctgg cgctgctcgc cgcatgcagc accgccacag
      901 tcgtcgccat ggccctgccc taccttctcg accgcctggg caaggacccg gcgtatggca
      961 gcggaccact ggccactgtg atccaggatc tgatctcgat cctggtctac ttcgccatcg
     1021 ctagcgcaat catggggtag caccacccct tgcattcggg ctcgccctga gcgaacatat
     1081 gttcgatgcg agccgaggcg tcgatcctgc acgccgatct cgacgctttc tatgcgtcgg
     1141 tggaacaacg cgacgacccc cggctgcggt cgcgcccggt gatagtcgga tcgggtgtgg
     1201 tgctggcagc gagctatgag gcgagggcct tcggggtccg caccgccatg ggagggggcg
     1261 tcgcccgccg gctctgcccc ggggcgatcg tggtgcagcc aaggatctct gcctactcag
     1321 aggcgagtaa ggcggtcttc gacctcttcc gagacaccac cccactcgtg gaagggctct
     1381 cgatcgatga agcctttctc gacgtaggcg gactgaaacg ggtgtcgggc tcgcctatcg
     1441 agatcgccag acgtctgcga agacgggtgt tcgacgaggt cgggcttccg atcacggtcg
     1501 gcattgcacg caccaagttc ctcgccaagg tggcgagcgg agtcgccaag cccaatgggc
     1561 tactcgtggt ggcggcgggg acagagttg
//
LOCUS       DATITW010000222         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_411223, whole genome shotgun sequence.
ACCESSION   DATITW010000222 DATITW010000000
VERSION     DATITW010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411223"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VLS86_03095"
     CDS             <1..1173
                     /locus_tag="VLS86_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HSO49508.1"
                     /translation="EVYILILPAMGIVSEILPTFSRKPLFGYSAVVFAGAAIAFLGFG
                     VWAHHMFASGITPVAQAAFGLATMTIAIPTGIKIFNWLGTMWMGRIRLSTPMLFAIGF
                     VAMFTIGGLSGVTHAVVPSDWQQTDTYYIVAHFHYVLFGGAIFGLFGGLYYWFPKLTG
                     RVMNEKLGKTHFWLQMLGFNLTFAPMHWLGLQGMVRRTWKYAPETGLEPWNLAVTVGA
                     FIIAFSIAVFIVNWVTSKRRGQPSGLDPWDARTLEWMVPNPTPEYNFGVPPVVIGRDH
                     FWHLKYAEGPDGRAVRRPEADEIVAKIEHEQLNPASPIHLPNPSYFPFFMALGFPVIF
                     YGVIYHTALWGKAMILIGVLIALISVIGWAIEPLEEPHQGGHDDAAQAEEHEEAHG"
     gene            1166..1789
                     /locus_tag="VLS86_03100"
     CDS             1166..1789
                     /locus_tag="VLS86_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HSO49509.1"
                     /translation="MADVLHEAPLDHPEHESTGVDNRKLGMWLFLSSEFLFFGALISN
                     YLLYSNRSGFAGTYPSEIYDIPFTSVSSFVLLMSSLTMVLAHNALTRGDMNGTRTWLF
                     ATAALGSVFLGGQVFEFTEFIVNYDMKLTSNPAASAFYVLTGFHGTHVAIGVIMLLSL
                     WGISRRPGGLSEKHGMNLELVGLYWHFVDIIWIVIFTVVYLLSVPPT"
     gene            1786..2094
                     /locus_tag="VLS86_03105"
     CDS             1786..2094
                     /locus_tag="VLS86_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HSO49510.1"
                     /translation="MTTEHHHPTAAQYWKIAVLLGVLTAIEVALYYVNLSYDLGSLNA
                     ILLIVLATLKFVIVVAVYMHLRYEKATLSRFFTGGFILAASLYTVVLAAMGVLAIRGS
                     "
BASE COUNT          361 a          778 c          637 g          462 t
ORIGIN      
        1 gaggtgtaca tcctgatcct ccccgccatg gggatcgtgt ccgagatcct tcccacgttc
       61 tcccgaaaac cgctgttcgg gtactcggcc gtggtcttcg ccggcgccgc catcgccttc
      121 ctcgggttcg gggtgtgggc ccaccacatg ttcgcctccg ggatcacacc cgtcgcccag
      181 gccgcgttcg gactcgccac catgaccatc gccatcccta ccgggatcaa gatcttcaac
      241 tggctgggca cgatgtggat ggggaggatc cggctctcca cgccgatgct gttcgccatc
      301 ggcttcgttg ccatgttcac gattggcggg ctgtccgggg tcactcacgc cgtcgtgccc
      361 tcggattggc aacagaccga cacctattac atcgtggccc acttccacta cgtgttgttc
      421 ggcggcgcca tcttcgggct gttcggcggc ctctattact ggttccccaa gctcaccggg
      481 agggtgatga acgagaagct gggcaagacc cacttctggc tgcagatgct gggcttcaac
      541 ctcaccttcg cccccatgca ctggctgggg cttcagggca tggtgcgccg cacctggaag
      601 tacgcaccgg agaccgggct cgagccgtgg aacctggcgg tgaccgtcgg cgccttcatc
      661 atcgccttct cgatcgcggt cttcatcgtc aactgggtga ccagcaaacg ccgcggccag
      721 ccctcaggcc tcgatccctg ggacgcccgg accttggaat ggatggtccc caacccgacc
      781 ccggagtaca acttcggagt gccgccggtg gtaatcgggc gcgaccactt ctggcatctc
      841 aagtacgccg aaggccccga cgggcgagcg gtgcgcaggc ccgaggccga tgagatcgtc
      901 gccaagatcg agcacgaaca actcaacccg gcgtcgccga tccatctccc caatccctcg
      961 tacttcccct tcttcatggc gttggggttc cccgtcatct tctacggggt catctaccac
     1021 accgcactct ggggcaaggc catgatcttg atcggggtgc tcatcgccct gatctcggtc
     1081 atcgggtggg ccatcgagcc gctggaggaa ccgcatcagg gaggccatga cgacgcggcc
     1141 caagccgaag agcacgagga agctcatggc tgacgtcctc cacgaggctc ccctcgacca
     1201 cccggagcac gagtcgacgg gtgtcgacaa ccgcaagctc gggatgtggc tgttcctgag
     1261 ctcggagttc ctgttcttcg gcgcactgat ctcgaactac ctgctgtact ccaaccggtc
     1321 gggattcgcc gggacctatc cctccgagat ctacgacatc cccttcacct cggtcagctc
     1381 cttcgtgttg ttgatgagct cgctgacgat ggtccttgct cacaacgcac tcacccgagg
     1441 cgacatgaac ggcacccgga cctggttgtt tgccaccgcc gcgttggggt cggtgtttct
     1501 cggcggtcag gtcttcgagt tcaccgagtt catcgtcaac tacgacatga agctgacctc
     1561 caacccggca gcctcggcgt tctacgtcct caccggcttt cacggcaccc acgtggcgat
     1621 cggggtgatc atgttgctct cgctgtgggg gatctcacga cgcccgggcg gtctcagcga
     1681 gaagcacggg atgaacctcg agctggtcgg cctgtactgg cacttcgtcg acatcatctg
     1741 gatcgtgatc ttcaccgtgg tctacctgct ctcggtgcct ccgacatgac caccgagcac
     1801 caccacccca ccgccgccca atactggaag atcgcggtcc tgctcggggt gctaaccgcc
     1861 atcgaggtcg ccctgtacta cgtgaacctc tcctacgacc tggggtcgct caacgcgatc
     1921 ctgctcatcg tcctggccac tctgaagttc gtcatagtcg ttgccgtcta tatgcatctc
     1981 cgctacgaga aggcgaccct cagccggttc ttcaccgggg gcttcatcct ggcggcctcc
     2041 ctgtacacgg tggtcctcgc cgccatggga gtcctcgcca tcaggggatc ctgatgccac
     2101 tgccaccctg gcacccgcac ttcgatctct tcctcttggt cttcgttctc ggcttcgggt
     2161 actggtacgc cgacacccgc ctccgccctc tgatcgcgcc ggaggcgtct ccggcaaccg
     2221 ggggccagcg ctggcaat
//
LOCUS       DATITW010000223         1668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_211059, whole genome shotgun sequence.
ACCESSION   DATITW010000223 DATITW010000000
VERSION     DATITW010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211059"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /gene="rpsE"
                     /locus_tag="VLS86_03110"
     CDS             complement(<1..435)
                     /gene="rpsE"
                     /locus_tag="VLS86_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HSO49511.1"
                     /translation="MAEVQYDERVITINRVAKVTKGAKRFSFTALVVVGDGNGRVGVG
                     YGKAREVPSAIQKGMEAARKAMVLIPMAGQTVVHQAVGIHGASRVLIKPAAPGTGVIA
                     GGAVRQILEAAGIRDALAKSLGSPTHINVAQAAMNALTGQRRP"
     gene            complement(444..794)
                     /gene="rplR"
                     /locus_tag="VLS86_03115"
     CDS             complement(444..794)
                     /gene="rplR"
                     /locus_tag="VLS86_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012164970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HSO49512.1"
                     /translation="MKNRLEARRRRHVRVRKAVVGTSERPRLAVFRSNRYLYAQVIDD
                     KTGRTLAAASSQEASLRSKTLSVETATEIGKLVAERAKSAGVGAVVFDRGGFTYHGRI
                     KALADAARASGLEF"
     gene            complement(791..1345)
                     /gene="rplF"
                     /locus_tag="VLS86_03120"
     CDS             complement(791..1345)
                     /gene="rplF"
                     /locus_tag="VLS86_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HSO49513.1"
                     /translation="MSRIGKKPITVPSGVEVTVDGVRVGVKGPRGRLERVFPENMVFS
                     MEKTEEAGTVLTVSRPDDQRDNRALHGLSRALLANMVQGVAEGFRRELQIVGVGYRAT
                     LKGSTIELQLGFSHPVSVDAPEGITFEVPEPTRIIVSGVDKEQVGQVAADIRKLRPPE
                     PYKGKGVRHLNEQVRRKAGKAGKR"
     gene            complement(1347..>1668)
                     /gene="rpsH"
                     /locus_tag="VLS86_03125"
     CDS             complement(1347..>1668)
                     /gene="rpsH"
                     /locus_tag="VLS86_03125"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HSO49514.1"
                     /translation="MPTSHLREGVARILASEGYIDRFEVASEAVKSNLTVFLRYGPRR
                     APAISGLKRISRPGHRVYRPADALPRVQGGLGVAVVSTSQGLLPDREARRRKLGGEVL
                     CEVW"
BASE COUNT          297 a          540 c          527 g          304 t
ORIGIN      
        1 ggggcgccgt tggccggtaa gggcattcat ggcggcctga gccacattga tgtgggtcgg
       61 ggagcccagc gatttggcga gggcatcacg aatcccggcc gcctcgagaa tctggcggac
      121 tgcccctccc gcgataacac cggtaccggg tgcggctggc ttgatgagaa cccgcgacgc
      181 accgtgaatg cccaccgcct ggtgaaccac agtttgaccg gccatcggga tcagcaccat
      241 tgctttgcgg gccgcttcca tccccttctg gatggcactg gggacctcgc gggcctttcc
      301 ataaccgact cccacccgac cgttgccgtc gccgacaacg accaaagcgg taaagctgaa
      361 ccgcttggca cccttggtga ccttggcaac gcggttgatg gtgatgactc tctcgtcgta
      421 ttggacctca gccatggtta ctcctagaac tccaagccgg atgcccgggc agcatccgcc
      481 agagccttga tgcgaccgtg ataggtgaag ccaccacgat caaagacgac ggccccgacc
      541 ccggcgctct tggcgcgctc ggcgacgagt tttccgatct cggtagcggt ttccaccgac
      601 agagtcttcg atcggagaga cgcttcttgg gacgaggcag ctgccagggt gcggccggtc
      661 ttgtcgtcga ttacctgggc gtacaggtag cggttgctcc ggaacaccgc caggcgggga
      721 cgctcggaag tgccgacgac agccttgcgc acgcgaacat gccggcggcg gcgtgcctcg
      781 aggcgattct tcatctcttc cctgctttcc cggctttgcg gcggacctgc tcattgagat
      841 gacgcacgcc tttgcccttg taaggctctg gtggtcgcag tttccgaatg tcggcggcga
      901 cctggccgac ctgctccttg tccacaccgg atacgatgat ccgggtgggt tcggggactt
      961 caaaggtgat gccctccggg gcgtcgaccg aaaccggatg gctgaaaccg agttgaagct
     1021 cgattgtcga acccttcaga gtggcgcggt atccgacgcc aacgatttgg agttcacggc
     1081 ggaaaccttc ggcgaccccc tggaccatgt tggccagcag cgcccgtgac aggccatgca
     1141 gtgcgcggtt gtcacgctgg tcatccgggc gcgaaaccgt caggaccgtg cccgcctctt
     1201 cggtcttctc catcgagaac accatgttct cgggaaacac ccgctcgaga cgtcccctcg
     1261 gccccttgac gccgacccga acgccgtcga cggttacttc gacaccggat gggaccgtta
     1321 tcggcttctt gccaatgcgg ctcatgtcac caaacctcgc acagaacttc gccgcccaat
     1381 ttgcggcgac gggcttcgcg gtcagggaga agaccctggg aggtcgagac gacggctacc
     1441 ccgaggccgc cctggactcg cggcaaagca tcggccggcc ggtagacccg gtgaccgggc
     1501 cgcgagatcc gcttgagacc ggagatggcg ggagcgcgac gggggccata gcgaaggaag
     1561 acggtcaggt tcgacttgac cgcttccgag gcaacctcga agcgatcgat gtagccctcg
     1621 gaggccagga tccgggccac cccttccctc aagtgggagg tcggcatc
//
LOCUS       DATITW010000224         1965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_571548, whole genome shotgun sequence.
ACCESSION   DATITW010000224 DATITW010000000
VERSION     DATITW010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571548"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..141
                     /locus_tag="VLS86_03130"
     CDS             1..141
                     /locus_tag="VLS86_03130"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HSO49515.1"
                     /translation="MILSDLEGLSLPEVAEVLDFPIGTVKSRVFRGRRLLAIQLGNQS
                     TT"
     gene            160..897
                     /locus_tag="VLS86_03135"
     CDS             160..897
                     /locus_tag="VLS86_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49516.1"
                     /translation="MHKHDLDLIASLADGTIEDATEARDLVASCDECRAEYHSQLEVL
                     EILAAAPPVAMSELEKAALHRDLWTELRTEPSTPAARAPWYRWAYVAAGLFVVVGLAG
                     ALSRLPFGAGDAASDTFVEVSSGLDGDAGQGGDIPLFDAPDAASGGEESVETTAAAES
                     LLLPFPELAEEARALRQSGSRSGQLMTDPVQIDQCLEKSGLADHVVVEELELDQRYLV
                     VMAREGSTDQTVTFIALASCEIVHRDG"
     gene            929..1195
                     /locus_tag="VLS86_03140"
     CDS             929..1195
                     /locus_tag="VLS86_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49517.1"
                     /translation="MEEYAARFAEFLEQIAIRIRSLTVDRVARGIRLTGLGILAATQG
                     LTALLFLLLTIFGALEIPLTTAGAFAVVGALLIGVGSYLWMKRT"
     gene            1203..>1965
                     /gene="trxB"
                     /locus_tag="VLS86_03145"
     CDS             1203..>1965
                     /gene="trxB"
                     /locus_tag="VLS86_03145"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HSO49518.1"
                     /translation="MGHEKLIIIGSGPAGLTAALYAARADLSPLVFEGVAAGGQLMIT
                     TDVENYPGFPDGILGPELMDQFRKQAQRFDARMQQVDVTEVDFGSRPFRIDVGADEYT
                     SDAVIIATGATAKWLGVPGEERLTGRGVSACATCDGFFFRDQELVVVGGGDTAMEEAL
                     FLTKFASKVHVVHRRDEFRASKIMAQRVMGHEKVDVIWNTVLKEIKGDTTVQSVTLES
                     TASGDQTEMPVNGVFIAIGHKPNTELFTEQIDLDEN"
BASE COUNT          354 a          607 c          648 g          356 t
ORIGIN      
        1 gtgatcctct ccgatctcga aggcctgtcg ctgcccgaag tggctgaggt tctcgacttc
       61 ccgatcggca cggtgaagtc gagggtgttc cggggtcgga ggctgctcgc catacagctg
      121 gggaaccaaa gcaccacatg agaccatcaa aggcttggaa tgcataagca cgacctcgac
      181 ctcattgcct ccttggccga cggcacgatc gaagacgcaa cagaggcccg tgacctcgtc
      241 gcttcctgcg acgagtgtcg cgccgagtac cactcccaac tcgaggtcct ggagatcctg
      301 gccgccgcgc caccggtggc catgagcgag ttggagaagg cggcactgca tcgcgacctg
      361 tggaccgagc tgcgcacgga gccctccacg cctgcggcca gggcgccgtg gtaccgctgg
      421 gcctacgttg cggccgggct gttcgtcgtc gtcggcctgg caggtgctct aagcaggttg
      481 cccttcggcg caggcgatgc ggcctccgac accttcgtcg aggtcagctc gggactcgac
      541 ggagatgccg gtcaaggcgg ggatatcccg ctgttcgacg ccccggacgc tgcttcaggt
      601 ggtgaggagt cggtggagac gacagcagcc gctgagtcgc tccttctccc gtttcccgag
      661 ctcgccgagg aggcccgcgc cttgcggcag agtggcagcc gttccggtca gttgatgact
      721 gatccggtcc agatcgatca gtgcctggag aagtcggggc tcgccgacca cgtcgtggtc
      781 gaggagctgg agctcgatca gcgctatttg gtggtgatgg cccgagaggg ctctaccgac
      841 cagaccgtca ccttcatcgc cctggcaagt tgcgagatcg tccacaggga tgggtgatcc
      901 ggcccggcca aactaccctc tcgggtccat ggaggagtac gccgcccgat tcgccgagtt
      961 cctcgagcag atcgcgatca ggatccggtc gctcacagtt gaccgcgtgg cccgcgggat
     1021 ccgcctcacc ggattgggaa tcctggcggc cacacagggg ttgaccgcgt tgttgttcct
     1081 gctgctgacg atcttcgggg cgctcgagat cccactcacc accgcagggg catttgcagt
     1141 cgttggagcc cttctgatcg gggtaggaag ctatctctgg atgaagagaa cctgaggaga
     1201 caatgggtca cgagaagctc atcatcatcg gatcgggacc ggccgggctg accgccgctc
     1261 tgtatgccgc ccgcgccgat ctcagcccgc tggtcttcga aggcgtggcc gccggcggtc
     1321 agttgatgat caccaccgat gtcgagaact acccgggctt tcccgatggg atcctcggcc
     1381 ccgagttgat ggaccagttc cgaaagcagg cgcagcgatt cgacgccagg atgcaacaag
     1441 tggacgtcac cgaggtggat tttggatcga gaccgttccg catcgacgtc ggggctgacg
     1501 agtacacctc cgacgcggtc atcatcgcca ccggagccac cgccaaatgg ctcggcgttc
     1561 ccggcgagga gcggctcacc gggcgcgggg tctccgcctg cgccacgtgt gacggcttct
     1621 tcttccgcga ccaggagctg gtggtggtcg gtggcggcga caccgcgatg gaggaggcct
     1681 tgttcctcac caagttcgcc tccaaggtcc acgtcgttca tcgtcgcgat gagttccggg
     1741 cgtccaagat catggcgcaa cgggtgatgg gccacgagaa ggtcgatgtg atctggaaca
     1801 cggtgctgaa agagatcaag ggcgacacga cggtccagag cgtcaccctg gagagcaccg
     1861 cgtcgggtga ccagaccgag atgccggtca atggcgtgtt catcgcgata ggtcacaagc
     1921 cgaacacaga gttgttcacc gaacagatag atctcgacga gaatg
//
LOCUS       DATITW010000225         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_771834, whole genome shotgun sequence.
ACCESSION   DATITW010000225 DATITW010000000
VERSION     DATITW010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771834"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /gene="ribD"
                     /locus_tag="VLS86_03150"
     CDS             <1..996
                     /gene="ribD"
                     /locus_tag="VLS86_03150"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HSO49519.1"
                     /translation="QRPHPNPRVGAVVVDAAGEIVGEGAHLGVGQSHAEVVALAGAGD
                     RARGSTLYVTLEPCTHHGLTPPCVDSILSSGVTRVVIGAIDPDPRVSGSGVAWLRREG
                     VDVVTDVLAAESEAVDPSYFHHRRTGLPRITLKLAMTLDGSVAAGDGSSRWITSEEAR
                     RDAHQLRATMDAVVIGAGTLRADDPLLDVRLDDPVSVQPTPVVIAGREPLPATGRIWA
                     RSPLVISTASIAVPSGEVVVVGGDGQWPDVVAAAHALADRGLLDVLVEGGPGLAGSWW
                     RQGVVSCGVFYVAGRMGGGRGVAPLGGEFETMADSRPVSIRDVRMVGPDIRIEFE"
     gene            997..1587
                     /locus_tag="VLS86_03155"
     CDS             997..1587
                     /locus_tag="VLS86_03155"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018850133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HSO49520.1"
                     /translation="MFTGIVAQQGSIANVEAVVDGRRLTIDLGHLDGLDVGDSISVNG
                     VCLTAVEVGPGRATVDVVLETLDRTNLGALQSGDPVNLELPMPANGRFDGHIVQGHVD
                     GVGEVLSVVSEGEGRRMTVRAPEGLMRYLVEKGSVTVDGVSLTVAALTEHGFEVALIP
                     HSLTVTTLGLRQAGQKVNLEVDVLAKYVERLLKANE"
     gene            1584..>2373
                     /gene="ribB"
                     /locus_tag="VLS86_03160"
     CDS             1584..>2373
                     /gene="ribB"
                     /locus_tag="VLS86_03160"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HSO49521.1"
                     /translation="MSFASVDEIIAAVGRGEMVIMVDDEDRENEGDLIIAADAVTPQQ
                     VGFMLRHTSGIICLPVIGERLDELDLPMMVARNTDARRTAFTVSIDAKEGTTTGISAS
                     DRWRTIQAVLSPETQPHDLARPGHLYPLRYEVGGVLKRAGHTEAAVDLARLAGRYPAG
                     VLAEIMNDDGSVARLAQLEEFAREHQLLIGTIADLISYRRHKEKLVERVVEARIPTEH
                     GTFTAVGFRSLVDDRQHIALVMGDIGDGESVLTRVHSECLTGDVF"
BASE COUNT          384 a          754 c          826 g          409 t
ORIGIN      
        1 caacgccctc atcccaaccc cagggtcggg gctgtcgtcg tcgacgccgc tggtgagatc
       61 gttggtgagg gcgcccatct cggggtgggg cagtctcatg ccgaggtcgt ggccctggcc
      121 ggggcggggg atagggcaag aggctcgacc ctctacgtca cgctcgagcc ctgcacccat
      181 cacggtctga caccaccgtg tgtcgactcg atcctctcct ccggcgtgac gcgcgtggtg
      241 atcggggcga tcgatcccga cccgagagtg tccggctcgg gagtcgcctg gctgcgccgt
      301 gaaggtgtcg acgtggtgac cgatgtcctc gccgccgaat ccgaagccgt ggatccgtcc
      361 tatttccatc accgaaggac cggtctgccc cgaatcacgc tcaaattggc catgaccctc
      421 gacgggtcgg tggcggcagg cgacgggtcc tcccgctgga tcacatccga ggaggcaagg
      481 agggacgctc accaactgag agcaacgatg gacgcggtcg tgatcggagc cgggacgctt
      541 cgcgccgacg acccgctgct cgacgtcaga ctcgacgatc ccgtctccgt ccaaccgacc
      601 ccggtcgtca tcgccggccg tgagccgctc ccggccaccg ggaggatctg ggccaggtcg
      661 cctctggtca tctccaccgc gtcgattgcg gtcccatccg gcgaagtggt ggtcgtggga
      721 ggcgacggcc agtggccgga cgtcgtggct gcggcgcatg ccctggccga tcgagggttg
      781 ctggacgtcc tggtcgaggg tgggccgggc ctcgccggct cgtggtggcg ccagggtgtg
      841 gtgagctgcg gggtcttcta cgtcgccggg cgtatgggtg gtggccgggg ggtggcaccg
      901 ctgggagggg agttcgagac catggcagac tcccggcctg tgagtatccg ggatgtgagg
      961 atggtcggcc ccgacatcag aatcgagttc gagtagatgt tcaccggaat cgtcgcgcaa
     1021 caaggttcga ttgcgaacgt cgaggcggtc gtcgacggga gacgacttac catcgatctc
     1081 ggccatctcg acggtctcga cgtcggcgac tcgatctcgg tgaacggggt ctgtctcacc
     1141 gcggtcgagg tcggtccggg ccgggcaacg gtcgacgtgg tcctggagac cctcgaccgg
     1201 accaatctgg gggctctgca gtcgggggat ccggtgaatc tcgagttgcc catgcccgcc
     1261 aacggccgct tcgacgggca catcgtccag ggccatgtcg acggtgtcgg cgaggtgctg
     1321 tctgtcgtct ccgaagggga gggtcggagg atgacggtca gggccccgga agggctgatg
     1381 cgctacctgg tcgagaaggg ctccgtgacc gtcgacgggg tcagtctgac cgttgccgcc
     1441 ctcaccgagc acgggttcga ggtcgccttg atccctcatt ccctgaccgt gaccactctg
     1501 ggtttgcgcc aggcaggtca gaaggtcaac cttgaggtgg acgtgctggc caagtacgtc
     1561 gagagactcc tgaaagcgaa cgaatgagct tcgcgtcggt tgacgagatc atcgctgcgg
     1621 ttggccgcgg ggagatggtc atcatggtgg acgacgagga tcgtgagaac gagggcgatc
     1681 tgatcatcgc cgccgacgcg gtcaccccgc aacaggtcgg gttcatgctc cgccacacca
     1741 gcggcatcat ctgtctcccg gtcatcggcg agcgtctcga cgagctggac ctgccgatga
     1801 tggtcgcccg caacaccgac gccagaagga cggcattcac cgtctccatc gacgccaagg
     1861 agggcacgac cacggggatc tcggcttcag accggtggcg gacgattcag gccgtgctga
     1921 gcccggagac gcagcctcac gacctggctc gccctggtca tctctatcca ctccgctacg
     1981 aggtaggcgg ggtcctgaaa cgagcagggc acaccgaggc cgcggtcgac ctggcccgac
     2041 tcgccggccg ataccccgcc ggcgtgctgg ccgagatcat gaacgacgac ggctcggtgg
     2101 cccgactcgc tcagctcgag gagtttgccc gggagcatca gctgctcatc gggactatcg
     2161 ccgacctgat ctcgtaccgg cgccacaagg agaagctggt cgagcgggtg gtcgaggcca
     2221 ggatccccac cgagcacggg accttcaccg ccgtcggctt tcgatcgctg gtcgacgacc
     2281 gccaacacat cgccctggtc atgggtgata tcggggacgg cgaatcggtt ctcacccggg
     2341 tgcactccga gtgcctcacc ggcgacgtat tcg
//
LOCUS       DATITW010000226         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_571584, whole genome shotgun sequence.
ACCESSION   DATITW010000226 DATITW010000000
VERSION     DATITW010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571584"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /gene="hutH"
                     /locus_tag="VLS86_03165"
     CDS             complement(<1..1350)
                     /gene="hutH"
                     /locus_tag="VLS86_03165"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HSO49522.1"
                     /translation="MIEIIVDGSPLTPGQVVEVARGRARARPGASLGDRMDPARAFVD
                     AVTESGEVVYGITTGFGALASTHIGREEAEALQLNLIRSHASGVGDPLPDELVRAILL
                     LRARSLAQGHSGVRPIIVERLLEMLERDILPVIPAQGSVGASGDLAPFAHMALPVIGE
                     GLVKVDGRVVPAAETDLEPIVLRAKEGLSLLNGTEGMAAMGALGLDEARTLVIATDVA
                     CALTVEAMMGSARPFREDIHRLRPHPGQIASAHRIAGLLEGSAIGAGHADDFDHAVQD
                     AYSLRCAPQVHGAVSDTLDHMEDVLTREMGSVVDNPIVFPETGEVVSGGNFHGQPLAF
                     VLDFATIAVSELASISERRTDRLLDPARSRGLPAFLAPNPGLHSGYMIGQYVQAALVA
                     ENKVLSHPASVDTIPTSAHREDHVSMSMGAALKAARAADFAVTIVAVELLAACQALDL
                     "
     gene            1415..1810
                     /locus_tag="VLS86_03170"
     CDS             1415..1810
                     /locus_tag="VLS86_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HSO49523.1"
                     /translation="MTDLTPLLSEPGASIAVIGATDRPGKYGGIIYRDLKRKGYKVFA
                     VNPYRDMVDGDPCWKSVKDLPETPSIADFVIPATRGLTVVDECEEAGILNIWLQPGAE
                     SRELMDKLEAGPFTWLAHACIMVRASIAG"
     gene            complement(1832..>2532)
                     /locus_tag="VLS86_03175"
     CDS             complement(1832..>2532)
                     /locus_tag="VLS86_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HSO49524.1"
                     /translation="LTGEKAYISNAPGADVYTLFARTTAAAGSRGVTAFVVTSDMEGL
                     SGEPIEMISPHPLGRIRLDEVFVPASHVIGQVDQGFRVAMETLDLFRPSVGAFALGMA
                     EAALRVATGHATTRTAFGAPIADFQGVSHQLANVRLDIEAAELLVYSAAAAHDSGDTE
                     SLTGKAAMAKLHATETAQKAVDTAIQVLGARGLEADSTLAHLYMEVRAPRIYEGTSEI
                     QRNIIARELFNGRI"
BASE COUNT          419 a          836 c          879 g          398 t
ORIGIN      
        1 caggtcgagc gcctggcatg cggcgagcag ctcgaccgcg acgatcgtca ccgcgaaatc
       61 ggcggcgcgg gcggccttga gcgcggcgcc catgctcatg ctgacgtgat cttcgcggtg
      121 cgccgacgtc gggatggtgt ccaccgacgc cggatgtgac agcaccttgt tctcggcgac
      181 cagcgccgcc tggacgtact ggccgatcat gtagccggag tggaggcccg ggttgggggc
      241 gagaaaggcc ggcagcccgc gagatcgggc agggtcgaga agacggtcgg tccttctctc
      301 cgagatcgag gccaactccg aaaccgcgat ggtggcgaag tcgagcacga acgccagggg
      361 ttggccgtgg aagttgcctc cggacaccac ctcacccgtc tcggggaaga cgatcgggtt
      421 gtcgaccacg gagcccatct ccctggtgag cacatcctcc atgtgatcga gggtgtcgga
      481 gacggcgcca tggacctggg gggcgcagcg cagggaatag gcatcctgaa ctgcgtggtc
      541 gaagtcgtcg gcgtggccgg cgccgatggc gcttccttcc agcaacccgg cgattcggtg
      601 ggcggacgcg atctggccgg ggtgggggcg aagccggtgg atgtcctccc gaaacggccg
      661 ggcgctcccc atcatcgcct ccacggtgag ggcgcaggcg acgtcggtgg cgatcaccag
      721 ggtccgagcc tcgtcgagcc cgagcgcacc catggctgcc ataccctcgg tgccgttgag
      781 cagcgagaga ccctccttgg cgcggaggac gatcggctcg agatccgtct ccgcggcagg
      841 caccacccga ccgtcgacct tgaccagtcc ctcaccgatc acgggcagcg ccatgtgggc
      901 gaacggggcg agatcgccag acgccccgac ggatccctgg gccgggatga cggggaggat
      961 gtcccgctcc agcatctcca gcagccgttc gacgatgatc ggcctgacgc cggagtggcc
     1021 ttgggccagg ctccgggccc ggagcagcag gatggcgcgg accaactcat ctggaagtgg
     1081 atccccgact ccagaggcgt gcgacctgat caggttgagc tggagcgcct cggcctcctc
     1141 gcgaccgatg tgggtggaag cgagtgcgcc aaacccggtg gtgatcccgt aaacgacctc
     1201 acccgattcg gtcaccgcgt cgacgaacgc ccgtgccggg tccatccgat ccccgagcga
     1261 ggccccgggg cgggcccgcg cccggcctcg ggcgacctcc accacctgac ccggagtcag
     1321 cgggctcccg tcgacgatta tctcgatcac cccggtcacg gtaccgagtc gattacctgg
     1381 aagccgggca ggtcgaatac cgttgcccca agtgatgacc gacctcacac ccttgctctc
     1441 ggaaccgggc gcgtccatcg ccgtcatcgg cgccaccgat cgtcccggca agtacggggg
     1501 gatcatctac cgcgacctga agcgaaaggg ctacaaggtg tttgcggtca acccctatcg
     1561 agacatggtc gacggcgatc cctgctggaa atcggtcaag gacctcccgg agacaccgag
     1621 catcgccgat ttcgtgatcc cggccactcg tgggctgacg gtcgtcgacg agtgcgaaga
     1681 ggccggaatc ctcaacatct ggctgcaacc cggcgccgag tcccgagagc tcatggacaa
     1741 gctcgaagcg gggccgttca cctggctggc ccacgcctgc atcatggtcc gggccagcat
     1801 cgcaggctag ggcgacgcta gggcatcgtc ctcagatccg cccgttgaac agctcccggg
     1861 cgatgatgtt gcgctggatc tccgaggttc cctcgtagat ccggggcgcc ctgacctcca
     1921 tgtagaggtg ggcgagggtc gagtcggctt cgaggccccg cgccccgagc acctggattg
     1981 cggtgtccac cgccttctgc gcggtctcgg tggcatgcag cttggccatg gccgccttgc
     2041 cggtgaggct ctcggtgtcg cccgaatcat gggccgctgc cgcggagtag acgagaagct
     2101 cggctgcctc gatgtcgagt cgcacattcg ccagctggtg ggagacccct tggaagtcgg
     2161 cgatgggcgc accgaacgcg gtgcgggtgg tggcgtggcc ggtggcgacc cggagcgcgg
     2221 cctcggccat ccccagcgca aacgcgccga cactgggtcg gaacagatcg agggtctcca
     2281 tggcgacccg aaaaccctgg tcgacctggc cgatgacgtg gctcgcgggc acgaacactt
     2341 catcgagccg gattcggccc agcggatgtg gcgagatcat ctcgatcggc tccccactga
     2401 ggccttccat gtcgctggtg acgacaaagg ccgtcactcc gcgggagccg gcggccgcgg
     2461 tggtgcgggc gaacagggtg tacacgtctg ctccgggtgc gttggagatg taggcctttt
     2521 ccccggtgag gc
//
LOCUS       DATITW010000227         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_411305, whole genome shotgun sequence.
ACCESSION   DATITW010000227 DATITW010000000
VERSION     DATITW010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411305"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VLS86_03180"
     CDS             <1..230
                     /locus_tag="VLS86_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSO49525.1"
                     /translation="IRHKNRVLDDWCSTIGRDPAEIERSCGANPLKPQTGDGYVDAGA
                     SLITLSFDGRTGYDLSPVADWISWRDERNRP"
     gene            complement(265..921)
                     /locus_tag="VLS86_03185"
     CDS             complement(265..921)
                     /locus_tag="VLS86_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HSO49526.1"
                     /translation="MGDIALGIFAILAGLLFCFRGYLAMRFVIPIWGAFSGFILGAGL
                     VASLSAEGFLVSVLGWVVGAVLALVFGLFAYLYYEVAVLLAMSAIGFALGTSVMVALG
                     VTWSWLIILVGLAVGIVLAAVAIVGDLPMTLLAVLTAFGGASVAVAGTMLLFGVISID
                     DFDSAATTQRLDDDWWWYAMFVVLAVAGIIAQIRSTVRLRASLREAWAESGGWQFRTT
                     "
     gene            1151..>1500
                     /locus_tag="VLS86_03190"
     CDS             1151..>1500
                     /locus_tag="VLS86_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HSO49527.1"
                     /translation="MADFTGLTTFDPVPSLQHIESVTVTVTTRPPEGSIVGHLVAEEG
                     DLPPELELDRDTLRAAGFEGKTGQTILIPRVGSTTLVLVGIGSLSGLDADGLRDAAAA
                     FARATETRGDLALA"
BASE COUNT          320 a          508 c          472 g          200 t
ORIGIN      
        1 tcatccggca caagaaccgg gtgctcgatg actggtgctc caccatcggc cgggatccgg
       61 ccgagatcga acggtcgtgc ggggccaacc cgctcaaacc gcagaccggc gacggatacg
      121 tggacgccgg ggccagcctg atcacgctct ctttcgacgg ccgcaccggg tacgacctct
      181 ccccggttgc cgattggatc tcgtggagag acgagagaaa ccggccctag aggtcagcac
      241 acgacccggc cgccgggtca gcggtcaagt agtgcggaac tgccagccac ccgactcagc
      301 ccaggcctcg cgaagcgagg cacgcaaccg gaccgtgctc cgaatctgag cgatgatccc
      361 ggccacggcg aggacgacaa acatggcgta ccaccaccaa tcgtcgtcga gacgctgggt
      421 ggtggccgcg gagtcgaagt cgtcgatcga gatgacacca aacaggagca tggtcccggc
      481 gaccgccaca ctcgccccgc cgaaggcggt gagcaccgcc agcagcgtca ttggcaggtc
      541 tccgacgatc gcgaccgcgg cgagaacgat ccccactgcg agcccaacca ggatgatcag
      601 ccaggaccag gtgaccccga gggccaccat cacactcgtg cccagggcga agccgatggc
      661 cgacatggcg aggagaaccg ccacctcgta gtagaggtag gcgaacagac caaagaccag
      721 agccagcacc gcaccgacga cccagcccag gaccgatacc aggaagccct cggccgacag
      781 ggaagcgacg agcccggcgc cgagaatgaa tcccgagaat gcgccccaga tcgggatcac
      841 gaatcgcatg gcgagataac cgcggaaaca gaagagcaat ccggcgagga tcgcgaagat
      901 tcccagagcg atgtcgccca tcagccaacc tcctcgaagg tgggtctcat cccaacaggg
      961 tcagactatg ccgacgatca accgcccgaa gggcattcca aaaggtgctt gttccgctcc
     1021 atccggcgaa aggcgaagat ccccggtggt cgctccaacg gctcgcaggt tgccggtcca
     1081 gcggtggaag caggtgatag gttccgatcc cgattcgacg gcctccgaac cgaaaggcgg
     1141 gtagcaacag atggctgact tcacgggatt gacgactttt gaccccgtgc cttccctcca
     1201 gcacatcgag tcggtcacgg tcaccgtcac cacgcgccct cccgagggct ccatcgtcgg
     1261 ccacctcgtt gccgaagagg gcgaccttcc tccagagctc gaattggacc gggacacgtt
     1321 gcgcgctgcc gggttcgagg gcaagacggg ccagacgatc ctgatcccca gggtcgggag
     1381 cacgaccctg gtcctggtgg gcatcggttc gctgtcgggt ctcgacgccg acgggctgcg
     1441 ggacgccgcc gcagccttcg cccgtgccac tgagacccgt ggcgacctgg cgctggcaat
//
LOCUS       DATITW010000228         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_451342, whole genome shotgun sequence.
ACCESSION   DATITW010000228 DATITW010000000
VERSION     DATITW010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451342"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1306)
                     /locus_tag="VLS86_03195"
     CDS             complement(41..1306)
                     /locus_tag="VLS86_03195"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HSO49528.1"
                     /translation="MSLSNLADHYWLTYSEYKPTIATVRGEHRFDDQLPLFDDDWLGH
                     ISEELRIINSSATAIDPTTLHFQDRITRDLLIHETGVWGSEIEDRFMVAAVDPYLGPH
                     TRLLSDTMQNTVTDPGQAEALLARYAKVGTHLASALAMIRANAAEGMTPPRAALDRVI
                     EQLDGYLASSFDSDPFLQLDLPEAHAAGWKDRAERLVRDVVKPAFAHYRDGLIEHVAP
                     LARPPEKSGLVWMSNGEELYGHLIHKYTQLPRTAEEIHQVGLEWATEVLVEQWQGIGE
                     KALGLSTIDEIFERLHRDPEFRFESEEEMLDHGRATIARAWAAVDDWFGARPATPCQV
                     VPVPASIAPAMPPAYYMQPPPDRSREGTYFLNTYKPQERDRFEYESAHFHEAIPGHHF
                     DRSLASELEDIPLFRRYAQVYAHTEGWGL"
     gene            1471..2253
                     /gene="fabG"
                     /locus_tag="VLS86_03200"
     CDS             1471..2253
                     /gene="fabG"
                     /locus_tag="VLS86_03200"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HSO49529.1"
                     /translation="MTVHTQRIFTNQALADRVVLVTGASGGIGSAIARGCAAAGADVA
                     VGYLSDHEGAARTVEEVTALGRRSRAFPADITDVTAVDQMVSGALDEFGRIDALVNNA
                     GIMPETPWSEMSVDEWRRVVDVDLTGAFICSKAVLPSMVERGSGSIVMIASRLGQIGF
                     AGVSHYAAAKAGLLGLTKSLAKELGPQGIRVNAVAPGVTITDMTADITGGEAGAKRLA
                     ELPAGRFATAAEVADSVIVLLSDAATLFHGQTLCPNGGGHMP"
     gene            2281..>2647
                     /locus_tag="VLS86_03205"
     CDS             2281..>2647
                     /locus_tag="VLS86_03205"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HSO49530.1"
                     /translation="MTAEQAVHRVPDGATLMVGGSGAGHAVPQAFIDALARVFSTEGH
                     PRDLTTVRVVGLGDFADRGFSQLALPGLMKRTIGSNIGNEPRLGELVERGEIESYSFP
                     QGVLSQLCREIAAGRPGLVT"
BASE COUNT          448 a          804 c          933 g          462 t
ORIGIN      
        1 cgctggaata gagacccatc tcatccgcga ggcgctctgc ttacagcccc cagccctcgg
       61 tgtgggcata gacctgcgcg tagcggcgga acagggggat gtcctcgagc tcgctggcga
      121 gtgagcggtc gaaatgatgc ccggggatgg cctcgtgaaa gtgggccgac tcgtactcga
      181 accggtcccg ctcctgtggc ttgtaggtgt tgagaaagta ggtgccctca cgtgaccggt
      241 cgggcggcgg ctgcatgtag taggcggggg gcatggccgg ggcgatcgaa gccgggaccg
      301 gcaccacctg gcacggcgtc gcggggcggg ccccgaacca atcgtcgact gcggcccacg
      361 ctcgggcgat cgtcgcccgg ccatggtcca gcatctcttc ctcggattcg aagcgaaact
      421 cggggtcccg gtgcaggcgc tcgaagatct cgtcgatggt gctcagcccg agcgccttct
      481 cgccgattcc ctgccactgc tcgacgagga cctcggtggc ccattccaga ccgacctgat
      541 gtatctcctc ggcagttcgg gggagctggg tgtacttgtg gatgaggtgg ccgtagagct
      601 cctcgccgtt gctcatccag accaggcccg acttctccgg tgggcgggcc aggggagcga
      661 catgttcgat cagaccatcg cgatagtggg cgaatgcggg cttgaccacg tctctgacca
      721 gtctttccgc tcggtccttc caacccgcgg cgtgagcctc tgggagatcg agctggagaa
      781 acgggtcact gtcgaacgag gaggcgagat agccatcgag ctgctcgatg acgcgatcca
      841 gtgcggcgcg gggtggtgtc atcccctcgg ccgcattggc tcggatcatg gccagggcgc
      901 tggcgagatg ggtgccgacc ttggcatatc gggcgagaag ggcctccgcc tgcccgggat
      961 cggtgacggt gttctgcatg gtgtcggaga gaaggcgggt atgtggcccg agatacgggt
     1021 cgaccgcggc caccatgaag cggtcttcga tctcgcttcc ccatacccct gtctcatgga
     1081 tgaggagatc acgagtgatc cggtcctgaa agtggagcgt ggtgggatcg atggccgtgg
     1141 ccgaggagtt gatgatccga agctcttcgg agatgtggcc gagccagtcg tcgtcgaaca
     1201 ggggaagctg gtcgtcgaac cgatgctcac cgcgaacggt ggcaatggtg ggtttgtact
     1261 ccgagtaggt gagccagtag tggtcggcca ggttggaaag cgacatcaag ttgccccatc
     1321 gactttcaca cgcaccgtac cgcatgccaa gcaggaaatt tgcgtcgact tgccggcgac
     1381 gggacgtccc acccggtcga tccggggccg gagcgacctt tgatacattg ggccgggcat
     1441 gcgcaacgac acgtcacccg gtatctgtgg atgacggttc acactcaacg catcttcacc
     1501 aatcaagccc tggcagatcg ggtggtcctg gtcaccgggg cgagtggtgg catcggctcg
     1561 gcaatcgcaa gagggtgcgc cgccgccggg gcggatgtcg cagtcggcta cctgtccgac
     1621 cacgagggcg cggcgcggac cgtagaggag gtcaccgcac tcggccgccg gtcgcgggcg
     1681 ttccccgcgg acatcaccga cgtcaccgcc gtggatcaga tggtctccgg ggccctcgac
     1741 gagttcggga ggatcgatgc cctggtcaac aacgcaggga tcatgcccga gaccccgtgg
     1801 agcgagatgt cggtcgacga atggcgtcgt gtcgtcgacg tcgacctgac cggggccttc
     1861 atctgcagta aagccgtgct cccctcgatg gtggagaggg gatcgggctc gatcgtgatg
     1921 atcgcatcgc gtctgggtca gatcggattc gccggcgtga gccactacgc agcggccaaa
     1981 gcgggtctgc tggggttgac caagtccctg gccaaagagc tgggcccgca ggggattcgg
     2041 gtcaacgcgg tggcgcccgg ggtgaccatc accgacatga ccgcagacat caccggaggc
     2101 gaggcaggag ccaagaggct cgccgagctg cccgcgggac ggttcgccac ggcggcagag
     2161 gtggctgaca gcgtcatcgt actcctttcc gacgcagcca ccctcttcca cggtcagacg
     2221 ctgtgcccca acggcggagg gcacatgccg tgacgagccc gggccggtga cgtggagctg
     2281 ttgacggcgg aacaagccgt gcaccgggtg ccggacggcg cgaccttgat ggtcggaggc
     2341 tcaggtgccg ggcatgcggt gccacaggcg ttcatcgacg cgctggcccg ggtgttcagc
     2401 accgagggcc atccgcgcga cctgacgacg gttcgggtcg tcggtcttgg cgacttcgca
     2461 gaccgcggtt tctcccagct ggccttgccc gggctgatga aacgcaccat cggctccaac
     2521 atcgggaacg agccccgact cggtgagctc gtcgaacgag gcgagatcga gtcctactcc
     2581 ttcccccagg gggtgttgtc ccagttgtgc cgggagattg cggcgggacg accgggactg
     2641 gtcactg
//
LOCUS       DATITW010000229         1633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_691822, whole genome shotgun sequence.
ACCESSION   DATITW010000229 DATITW010000000
VERSION     DATITW010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691822"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VLS86_03210"
     CDS             <1..324
                     /locus_tag="VLS86_03210"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSO49531.1"
                     /translation="DGAPVVPWMRAVVADGEVVGFVMLALRTENHPEPYLWRLLIDRL
                     HQRRGIGDRVIDLLADECRRMGDKTLLTSWTEGKGSPRPFYLAHGFEPTGRIVDEETE
                     ARKTL"
     gene            383..>1633
                     /locus_tag="VLS86_03215"
     CDS             383..>1633
                     /locus_tag="VLS86_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49532.1"
                     /translation="MAVVTQTLESGRQAVARRAWDEALETLTVADREETLSPQDLELL
                     ADAAWWAGRPDESVQALERAFAGYVDSDMPASAATVASLLAYLATRRLAFSIASGWRA
                     KAMRLLEGQPESGALAFVKLLDLVEALLHHGDIEAATRIADETIDVARRTGNRDAEIQ
                     ATVFKGAALVSSGLWREGLALVDEASASALSGDLSLRSASDVYCTTIAACRSLADFGR
                     AGEWTEEADRWMSEHSVGGYPGVCKVHRAELKRLKGSWGEAEEEARSACQELERYHIL
                     DGVGFAHGEVGEIRLLMGDLTGAEQAFNSAFEFGFEPQPGLALLMLARGQAEEAARTL
                     SRALARGEGNQQRDLLTRARLLPAQVKVALARDDLETARAATSELESISEEFDRPAFE
                     ATALTARVEIALHEGNADAALPDLD"
BASE COUNT          276 a          509 c          582 g          266 t
ORIGIN      
        1 gacggcgccc cggtagttcc ctggatgcgg gccgtggtgg cagacgggga ggtggtggga
       61 ttcgtcatgc tcgcgctgcg caccgagaac catcccgagc cctatctgtg gcgtctcctg
      121 atcgaccgcc tccaccagag acgtgggatc ggagatcggg tgatcgatct gcttgcagat
      181 gaatgccgac gaatgggcga caagaccctc ctcaccagtt ggaccgaggg aaagggctcg
      241 ccccggccct tctacctggc ccacggcttc gagccgacgg ggcggatcgt cgacgaagag
      301 acggaggctc gaaagacact gtgagtcgaa cgcatgggtt gaaacccgac gagatcggta
      361 gtgtgtcgga cccgggggtg aggtggcagt cgtgacccag actctcgaat cgggaagaca
      421 ggcggtggcg cgcagggcat gggacgaggc cctggagact ctgaccgtcg ccgaccgcga
      481 ggagacactg tctcctcagg acctcgagtt gctcgcagac gctgcctggt gggcgggccg
      541 ccccgacgag tcggttcagg cgctggaacg ggcgttcgcc ggttacgtcg actcggatat
      601 gccggccagt gccgccaccg tcgcctcgct gctcgcctat cttgcgacgc ggcgtctcgc
      661 gttctccatc gcgtccggct ggcgggcgaa agcgatgcgt ctcctggagg gtcaacccga
      721 gtccggcgcc ctcgccttcg tcaagttgct cgacctggtc gaggccctct tgcatcacgg
      781 cgacatcgag gcggcgacca ggatcgccga tgagacgatc gacgtcgcac ggagaaccgg
      841 aaaccgggac gcggagatcc aggccactgt gttcaagggt gctgcgctgg tctcgagcgg
      901 gctgtggagg gagggcctgg ccttggtcga cgaggcgtca gcctcggcac tatccggaga
      961 cctcagtttg aggtctgcca gcgacgtgta ctgcaccacc atcgctgcct gccgcagtct
     1021 cgccgatttc ggacgtgccg gggaatggac ggaggaggca gaccgatgga tgagcgagca
     1081 ctcggtcggc ggatacccgg gggtgtgcaa ggtgcatcgc gccgagttga agcggctcaa
     1141 ggggtcgtgg ggcgaggccg aggaggaggc ccgcagcgca tgtcaggagt tggagcgcta
     1201 tcacatcctc gacggcgtcg ggttcgccca cggcgaggtt ggggagatcc gacttctcat
     1261 gggcgatctc accggcgccg agcaggcgtt caacagcgcc ttcgagttcg ggttcgagcc
     1321 ccagcccggg ttggcgctgc tcatgctcgc tcgcgggcag gccgaagagg ctgctcgcac
     1381 cctgtcgcgg gccctggcca gaggggaggg aaaccagcaa cgcgaccttc tcacccgggc
     1441 tcggctgctg cccgcccagg tgaaagtggc cctcgccaga gacgatctgg agacggcccg
     1501 agccgcaacc tccgagttgg agagcatctc cgaggagttc gatcgtcccg cattcgaggc
     1561 cacggcactg accgcccgtg ttgagattgc gttgcacgag ggcaacgccg acgccgccct
     1621 tcccgacctg gat
//
LOCUS       DATITW010000230         1688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_91042, whole genome shotgun sequence.
ACCESSION   DATITW010000230 DATITW010000000
VERSION     DATITW010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91042"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VLS86_03220"
     CDS             complement(<1..1244)
                     /locus_tag="VLS86_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HSO49533.1"
                     /translation="MSGYLDAVRDGVVIFDGATGTNLQTVGLTADDFGGASLEGCNEL
                     LNLRRPDVIRDLHRSFLDIGIDVVETNTFGAFAVPLAEYDLASQSYEIAAAGAALARE
                     VVDGYVAGDGRARYVAGSIGPGTKFTTLGQITYRDMASAYEVQASALIDGGVDLFVIE
                     TQFDLLGAKAAIEGVRRAMRSAQVELPVQVQVTIELTGRMLPGTEIGAALTALEAMGP
                     DVVGLNCATGPSEMYEPLRHLSRHSTVPISAIPNAGLPHVEDGEMAYDLSPDELANHL
                     GRFVTDMGIGVIGGCCGTTPAHLAAVVDRVRPLPLAERSPDVVPSAASIYSQVPFDQD
                     TSFLIIGERANANGSRAFKESLLEDDVEGCIAVGQEQVKEGAHLVDLCVDYVGRDGVA
                     DMKKIAAAFATDVAAPVVLDST"
     regulatory      complement(1361..1447)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT          306 a          541 c          550 g          291 t
ORIGIN      
        1 tcggtcgagt cgaggaccac gggcgccgcc acgtccgtgg cgaaggcggc cgcgatcttc
       61 ttcatgtccg cgacgccgtc gcggccgacg tagtcgacgc agaggtcgac gaggtgagcg
      121 ccctccttga cctgttcctg accgacggcg atgcagcctt cgacgtcgtc ttccagcagc
      181 gactccttga aggcccggga accgttggcg ttggcccgct cgccgatgat caggaacgaa
      241 gtgtcctggt cgaacgggac ctggctgtat atggatgccg cagaaggcac cacgtccggg
      301 gatcgctcgg cgaggggcag ggggcgcacc ctgtcgacca ccgcggcaag atgagcgggc
      361 gtggtgccgc agcaaccgcc gatgaccccg atgcccatgt cggtgacgaa tctcccgaga
      421 tggtttgcga gctcgtccgg gctgagatcg tatgccatct cgccgtcctc gacatgtggc
      481 agcccggcat tcggtatcgc tgagatcggc accgtggagt gccgggagag atgtcgcagc
      541 ggctcgtaca tctccgatgg gccggtagcg cagttgagac ccaccacgtc cgggcccatc
      601 gcctcgagag cggtgagggc ggccccgatc tcggtgccgg ggagcatccg cccggtcaac
      661 tcgatggtga cctggacctg cacggggagc tcgacctgag ccgaccgcat ggcgcgacgg
      721 acaccttcga tggcggcttt ggcgccgagc aggtcgaact gggtctcgat cacgaagagg
      781 tcgactcccc cgtcgatcaa tgccgaagcc tggacctcgt aagccgaagc catgtcgcgg
      841 taggtgatct ggcccagagt cgtgaacttg gtgccgggac cgatggaacc ggcgacgtat
      901 ctcgccctcc cgtcgccggc gacataaccg tcgaccacct cccgggcgag ggcggcgccg
      961 gcggcggcga tctcatagga ctgcgacgcg aggtcgtact cggcaagggg cacggcaaac
     1021 gcgccaaagg tgttggtctc gaccacatcg atcccgatgt ccaggaacga ccgatggagg
     1081 tcgcgaatga cgtcgggccg gcggaggttg agcagctcgt tgcacccctc gagcgaagcc
     1141 ccgccgaaat cgtcggcggt gaggccaacg gtctggagat tggtcccggt cgccccatcg
     1201 aagatgacga ccccgtccct gacggcgtcg agatacccgc tcaacggggg cgtccttcac
     1261 gtctggccct acagaaaggc ggttcggcct cactcaccgg atactcctct gtttgtttct
     1321 ctcgaaacgc ttgaagagcg ccggaagcga ggcttcaggc aactcatcga tccaggcatt
     1381 ggcaccgcgc ttgcgccggt tgccgcggtt tcatcgggcc tagtccctca accgctcatg
     1441 atgagcgctt cgtcgactcc caggatattt gattgcagcg gataagtgca agacccgttg
     1501 tcggcccgca ttgacttgga acgtgatccg ggataccttt cccagtcacc atgagtgttg
     1561 gattgatgac cggaacgacg accacgaccg ccaggccgtg cgactcgacc gtgtaacagc
     1621 actcaccacc acacgaaccg agatccgcac cggcaaacgg gggcggatct ttttgattcg
     1681 caaccggt
//
LOCUS       DATITW010000231         1676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_331557, whole genome shotgun sequence.
ACCESSION   DATITW010000231 DATITW010000000
VERSION     DATITW010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331557"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VLS86_03225"
     CDS             complement(<1..580)
                     /locus_tag="VLS86_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit gamma"
                     /protein_id="HSO49534.1"
                     /translation="MATLASTQVAYPTEAMLPRPFRVTSLHAETEDTVTLELEPEDGI
                     PLSFHPGQFTMFYVFGIGEVPISISGDPHHPAALIHTVRSVGAVTEAIVGLGVGDSVG
                     IRGPDGVGWPVGEAEGKDLVVVAGGIGLAPLRPVVLDVLARRDRFGGVSIIYGARSPG
                     DLLFEDELRSWRSRFDVETEVTVDRSDDDWFGD"
     gene            complement(584..1003)
                     /locus_tag="VLS86_03230"
     CDS             complement(584..1003)
                     /locus_tag="VLS86_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HSO49535.1"
                     /translation="MARLTNTQLELFESLSRELHLAADEILFEEGGEADSFYVIVTGR
                     IGLELTSPGHPPMVIQTLGPGDLVGVSWFFPPYRWNWRARSQGETDLIAFDAVEVRRQ
                     CGLNRDLDLEVLGAVAAEAVRRLHGTRIQLLDLYRST"
     gene            complement(1036..>1676)
                     /locus_tag="VLS86_03235"
     CDS             complement(1036..>1676)
                     /locus_tag="VLS86_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSO49536.1"
                     /translation="GTCFCVSMGTGPRCTSGYDLVVTEVIDLDRHEFVIESGSEAGEE
                     VLSRLHGRAAADGDLVTVDQIVSQTAASMGRRMDTEGIKELLLENLEHPRWDDVAERC
                     LACTNCTLVCPTCFCSTTTDTVTLDGLSSTRTRSWDSCFTLDFSSLHGVPVRDSTKSR
                     YRQWMTHKLANWFDQFGSSGCVGCGRCITWCPVGIDITVEVAAIRDSLVPVT"
BASE COUNT          325 a          549 c          516 g          286 t
ORIGIN      
        1 cgtcgccaaa ccagtcgtca tccgaccgat cgacggtgac ctcggtctcg acgtcgaatc
       61 gactccgcca tgagcgcagc tcatcctcga agagcaggtc acccggcgat cttgcgccat
      121 agatgatcga cacccctccg aaacgatccc ggcgggccag cacgtcgagc accaccggac
      181 gaagtggtgc caggccgata cccccagcaa ccacgacgag gtctttgcct tcggcttccc
      241 cgaccggcca tcccacccca tccgggcctc tgattcccac actgtccccg acgcccaggc
      301 cgacgatggc ctcggttacc gcgcccaccg agcggaccgt gtggatcagg gccgccggat
      361 ggtgaggatc gcccgagatc gagatcggca cctcgccaat gccgaagacg tagaacatcg
      421 tgaattgacc gggatggaag gagagcggga ttccatcctc cggctccaac tcgagggtga
      481 ccgtgtcctc ggtctccgcg tggagcgagg tgacccgaaa ggggcgaggc agcatcgcct
      541 cggtggggta tgcgacctgg gtcgaggcga gggtggccaa tgctcaggtg ctccgataga
      601 gatcgagaag ctggatccgg gtgccgtgca gtcgccgcac cgcttcggcg gccaccgccc
      661 ccagaacctc gaggtcgagg tcccggttca atccgcactg acggcggacc tcgacggcat
      721 caaaggcgat gaggtcggtt tccccttggg aacgagctct ccagttccag cgataagggg
      781 ggaaaaacca gctgacacca accaggtccc ccggtcccag ggtctggatg accatcgggg
      841 gatggcccgg gctggtgagc tcgaggccga tcctgcccgt cacgatgacg tagaaggaat
      901 cggcttcgcc gccttcctcg aacaggatct cgtcagcggc cagatgcagc tcacgggaga
      961 gagactcgaa gagctcgagt tgggtgttgg tcagtctcgc caacaattca ctgctctctc
     1021 gtatcgtctg gaggttcatg tcaccggcac caatgaatcc cggatcgcgg ccacctccac
     1081 cgtgatgtcg atgcccaccg ggcaccaggt gatgcaccga ccgcatccga cgcatcccga
     1141 cgagccgaac tgatcgaacc aattggcgag cttgtgagtc atccactggc gatatctcga
     1201 cttggtcgag tcccgaaccg ggacaccgtg gagactggag aaatccagcg tgaagcatga
     1261 gtcccaactt cgcgtcctgg tggaagagag accgtcgagg gtgacggtgt cggtggtcgt
     1321 tgagcagaaa caggttgggc agaccagggt gcagttggtg caggcgaggc atctctccgc
     1381 cacgtcatcc cagcggggat gctccaggtt ctccaagagc agctctttga tcccttcggt
     1441 gtccatcctt cggcccatgc tggctgcggt ttggctcacg atctggtcga ccgtgaccag
     1501 atcgccgtcg gcggcggccc tgccgtgcag tctcgacagc acctcctcac ccgcttcgct
     1561 cccggactcg atgacgaatt cgtgccggtc gaggtcgatg acctcggtca ccaccaagtc
     1621 atagcccgac gtgcaacggg gcccggtccc catcgacacg cagaaacagg tgcccc
//
LOCUS       DATITW010000232         1775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_211575, whole genome shotgun sequence.
ACCESSION   DATITW010000232 DATITW010000000
VERSION     DATITW010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211575"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VLS86_03240"
     CDS             <1..268
                     /locus_tag="VLS86_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49537.1"
                     /translation="GFNGPRPELHAHLTDTHPDKVETVTSLSERRYYELACPSCDDSW
                     KQEIKPRLRDPGFLEEYAREIRVVAFDQFIYHLQAAHNKDEVDA"
     gene            261..695
                     /locus_tag="VLS86_03245"
     CDS             261..695
                     /locus_tag="VLS86_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HSO49538.1"
                     /translation="MPEVRGCDIPDHLHYWPEKHVWAALADGIVTVGITDIAQNLAHN
                     VLSALPKDVGRPAKKGKSLGTVESSKWVGPVTSPLDGEVVEANSLLTSDPGILNRDPY
                     GEGWYVRIRPDDWSRDSADLVTGEAGVAAYQAFLEAEGIVCQ"
     gene            692..1141
                     /locus_tag="VLS86_03250"
     CDS             692..1141
                     /locus_tag="VLS86_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HSO49539.1"
                     /translation="MSVWRGCDIPTDRTYDVEGDLWVQYEKDLVRLGLTDVGQTRMGR
                     MVTIRFKQPGRRVRAGGSVATVESAKWVGPVHTPFAAEVVEANEAAFARDILIANRDP
                     YVAGWISLIRPDPPGEPDRPLLRGEEAVATYKARIESLEVSCFRCED"
     gene            1141..>1775
                     /locus_tag="VLS86_03255"
     CDS             1141..>1775
                     /locus_tag="VLS86_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49540.1"
                     /translation="MATVMRVIDFGEVSPLRSQTLWHAIAYGVSAGSPPTLSFMRPSH
                     PYVSIGFHRRLGELDLEWCRATGLPVFRRMVGGGPVYLDGGQHFFQISVPEAMTKGTR
                     TQTIRSLLAPAVEAFRSVGIDAHLDEHSEISVGQAKICGHAAGQIGSAVVVVGNLITS
                     FDHQAAARIIATPTPTFAEAYLRLMKEYVRATPAAPEAFRAAAVESYANEL"
BASE COUNT          309 a          573 c          565 g          328 t
ORIGIN      
        1 cgggttcaac ggcccccggc ccgagctcca cgcccacctc accgacaccc atcccgataa
       61 ggttgagacg gtgaccagcc tcagcgaacg tcgctactac gagctggcct gcccgtcgtg
      121 cgacgactcc tggaagcagg agatcaagcc tcggctccgt gatcccgggt tcctcgagga
      181 atacgcccgt gagatccgcg tcgtcgcctt cgaccagttc atctaccacc tgcaagccgc
      241 ccacaacaag gacgaggtcg atgcctgaag tcagaggttg cgacattccc gatcaccttc
      301 attactggcc ggagaagcac gtgtgggccg ctctcgccga tggcatcgtg acagtcggca
      361 tcaccgacat cgcccaaaat cttgcccaca acgtcctctc tgctcttccc aaggacgtgg
      421 gtcgtcccgc caagaaggga aagagcttgg gcacggtgga gtcgagtaaa tgggtcggcc
      481 ctgtcacctc tcctctcgac ggcgaggtgg tagaggcgaa ctctctcctc acctccgatc
      541 ccgggatcct caatcgcgat ccctacgggg aggggtggta cgttcgtatc cgtcccgacg
      601 actggtcccg agactccgct gatttggtca ccggggaggc gggggtggcc gcctatcagg
      661 cgttcctcga ggcggaaggg atcgtctgtc agtgagcgtc tggcgtggct gcgacattcc
      721 taccgaccgc acttatgacg tggagggtga cctctgggtt cagtacgaga aggacctggt
      781 ccgcctcgga ctgaccgatg tcggccagac ccgaatgggt cggatggtga ccattcgttt
      841 caagcagccc ggacgcaggg tgcgggccgg gggatcggtt gccaccgtcg aaagcgctaa
      901 gtgggtaggc ccagtccaca ctcccttcgc cgccgaggtg gtggaagcca acgaggccgc
      961 cttcgccaga gacatcctca tcgccaatcg cgatccctat gtggcagggt ggatcagttt
     1021 gatccgcccc gacccgccgg gcgaacccga ccgcccgctg ctgcggggtg aggaagcggt
     1081 ggcgacgtac aaggcgcgga tcgaatccct cgaggtgagc tgctttaggt gtgaggactg
     1141 atggcgacgg tcatgcgagt gatcgacttc ggggaggtgt ccccgctccg ctcccagacc
     1201 ctctggcatg ccatcgccta cggggtctcc gcagggtccc cgccgactct ctccttcatg
     1261 cggccatccc atccttacgt ctccatcggg tttcatcgac ggctgggaga acttgacctc
     1321 gagtggtgtc gagccaccgg actcccggtc ttccggcgga tggtgggtgg tgggccggtc
     1381 tacctcgatg gcggccagca ctttttccag atctcggttc ccgaggccat gaccaagggc
     1441 acacggaccc agacgatcag gtctttgttg gcgccggcgg tggaggcatt cagatcggtg
     1501 gggatcgacg cccatctcga cgagcactcc gagatatcgg taggccaggc caagatctgc
     1561 gggcatgccg ccgggcagat tgggtcggcc gtggtggtgg tcggcaacct catcaccagt
     1621 ttcgaccatc aggccgccgc ccgcatcatc gccaccccca cccctacatt tgcggaggcg
     1681 taccttcggc tgatgaagga gtacgtgagg gccactccgg ccgctcccga ggcgttccgg
     1741 gcggcggcgg tggagtccta cgcgaatgag ctgga
//
LOCUS       DATITW010000233         1946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_131335, whole genome shotgun sequence.
ACCESSION   DATITW010000233 DATITW010000000
VERSION     DATITW010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131335"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1727
                     /locus_tag="VLS86_03260"
     CDS             204..1727
                     /locus_tag="VLS86_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSO49541.1"
                     /translation="MPNHPVLIVNPRSGNGRAAAIDLIHTAQQLDLETVTMQPGDDLA
                     ELSVRMVDGGCDHLMMAGGDGSLAIVAEVAMARDVPFSCVPVGTRNHFAMDLGLDRSH
                     PLQSLDAALDGVERLIDVGIVAGQFFLNNVSFGLYPRAIADPEYRSHRARSMADAAVD
                     SARNLESQLTLTSPDGRVVSDVEVLLISNNPYRFIGPPDFARRPALDTGALGIILADR
                     LSRVKREPAAFTRWETPTLTVHADPASIKVGVDGELRTLQAPIEVALEPKSLRVLVPR
                     QTQPKRFKESIEHLNERTIVHLSGLPTLPSRDEVTVRSPLLRQLDEVDEAVFERIAGW
                     ESPAMDRIMPALSQAASHSKIWMTVAVALAIVGGKKGRTTAVESLAAVGITSLRATVV
                     AKGLFRRSRPTDQVPEERRLPTPGSSSMPSGHTASGAAFARVVGAAYPGLRIPLNALA
                     AAIGFSRVYTGVHYPTDVIAGWLLGRGIGSLTQRVAATAWRVPRSPARPRVQENQRP"
     gene            1724..>1946
                     /locus_tag="VLS86_03265"
     CDS             1724..>1946
                     /locus_tag="VLS86_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HSO49542.1"
                     /translation="MIVDFARYQAGVRTSEPSKIADAAILAKQGPGFVLLALSEPSAE
                     ELKQLAVTFDLPPLAVEDGFEGHQRPKLED"
BASE COUNT          344 a          664 c          590 g          348 t
ORIGIN      
        1 tggtcctcgg gttttggctg tcgggtcagt tcctgatcac ccaggcggcg gcattgctca
       61 ttttcgccgg aatctgggcg atgatgagag gattcctcga catcacggcg ttcttcacgg
      121 cgagaagcgt cgccgacgca gttccgaccg agtgacacca actgtgactg gcatgacgat
      181 gagctggtcc taggaaaagt gccatgccga accatccggt actgatcgtg aacccgagaa
      241 gcggtaacgg gcgcgccgcc gccatcgacc tgatccacac cgcgcaacag ctggacctcg
      301 agactgtgac catgcagccc ggcgatgacc tcgccgaact atctgtccgc atggtggatg
      361 gagggtgcga ccacctcatg atggctggag gtgacggttc cctggccatc gttgccgagg
      421 ttgcgatggc ccgggacgtt cccttttcct gtgtaccggt gggcacccgc aaccacttcg
      481 ccatggatct cggcctggac cgcagccatc ccctgcagtc cctcgacgcc gccctcgacg
      541 gcgtcgagag gctgatcgac gtcggcatcg tggccgggca attctttctc aacaacgtgt
      601 ctttcggcct ctatccccga gccatagccg accccgaata ccgctcccac agagcccgga
      661 gcatggccga cgccgccgtc gactcggcca ggaatctcga atcccagctc acactcacct
      721 cgcccgacgg ccgcgtcgtc tcagacgtcg aggtgctgct catctctaac aacccgtacc
      781 gcttcatcgg gccgccggat ttcgccagac gccccgctct cgacaccgga gctctgggga
      841 tcatccttgc cgaccgccta tcgcgggtga aacgcgagcc ggcggcgttc accaggtggg
      901 agaccccgac actaaccgtt cacgccgatc ctgccagcat caaggtcggc gtcgacggcg
      961 agctgaggac gctccaggct ccaatcgaag tggcacttga gccgaagtcg ctgcgagttc
     1021 tcgtcccgag acagactcag ccgaagcgtt tcaaggagtc gatcgagcac ctaaacgaac
     1081 gcaccatcgt ccatttgagc ggcctgccca ccctgcctag ccgcgatgag gtgaccgttc
     1141 gatcccccct gcttcggcag cttgacgagg tcgacgaggc ggtgttcgag cggattgcag
     1201 gatgggaatc gcctgcgatg gaccgcatca tgcctgcgtt gtcgcaggcg gcatcacact
     1261 ccaagatctg gatgacggtg gcggtcgccc tggcaatcgt cggcggcaag aagggccgca
     1321 cgaccgcagt cgagagtctc gcggctgtcg gcatcacctc gcttcgcgcc accgtggtgg
     1381 cgaaagggct attccggcga agtcgaccga ccgatcaagt tcccgaggag cggcgtctcc
     1441 cgaccccggg atcatcctcg atgccctccg gccacaccgc gtcgggcgca gcattcgctc
     1501 gcgtggtcgg tgccgcatat ccggggctgc ggatcccact caatgcgctc gccgctgcca
     1561 tcggattctc gcgggtctac accggagtgc actaccccac cgatgtcatc gccggctggc
     1621 tcctcggtag aggcatcgga tcccttacgc agcgggtggc ggcaacggcc tggcgtgtcc
     1681 ctcgctcgcc tgcccgccct cgagtacagg agaatcagcg gccgtgatcg tcgacttcgc
     1741 ccgatatcag gccggggtcc gcacgagcga gccctctaag atcgccgacg ccgccatcct
     1801 ggccaaacag gggcccggat tcgtgctgct ggctctcagc gagcccagtg ctgaggagct
     1861 gaagcagctc gctgtgacat tcgacctgcc tccactagcc gtggaggacg ggttcgaggg
     1921 ccatcagcgt cccaaactgg aagact
//
LOCUS       DATITW010000234         3712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_331803, whole genome shotgun sequence.
ACCESSION   DATITW010000234 DATITW010000000
VERSION     DATITW010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331803"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..892
                     /locus_tag="VLS86_03270"
     CDS             65..892
                     /locus_tag="VLS86_03270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSO49543.1"
                     /translation="MRADIGAFGVWRMVSGITPELAEQVEALGYGAIWLGGSPTGDLE
                     AVEVVLDATERIPVATGIVNMWREDAATVAESYRRVSGRHAGRFLLGVGVGHREATQE
                     YHKPYDKMVDYLDRLDAAAVPQEDVVLAALGPRALTLAAERTSGAHPYLTTPRHTDQA
                     RLLIGRDPLLAPEQKVVLETDATLARAIGRPVVSRYLTRINYRSNLLREGWAESDLEA
                     GGSDRLVDALVLHGTSEDIAAGLTAHLDAGADHVAIQVLGDDPLDGYRQLSGVLIAV"
     gene            940..1926
                     /locus_tag="VLS86_03275"
     CDS             940..1926
                     /locus_tag="VLS86_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49544.1"
                     /translation="MQKGGRMGHITFVHGIMNQPSPARLLGSWKRHLADGGDGLDLDV
                     YGVTSSVVYWADVMYPATTDAGTTQESIEYEGLESGALTQVDESYIEEAGGEEKAFIE
                     AMIANYGLDHPDEADTPADTSSATQPAGEVEAEVRLTAAAALEAVPLPWFIKRPMMRV
                     LLRDVHHYLFNTSHSPRPGSKFKVRDEVRGRFVSDLGAVDNGPHVVVAHSLGTVIAYD
                     CLKRVADCKKIDLLVTLGTPLGLSEVQDKLKPEWSKDDGFPDGLPRWVNVVDRLDPVC
                     AADPKIANDYQHKKQAVVVDEVVSNGGVMRHPPDKYFRQPVVQAAIKEGLGL"
     gene            1933..3498
                     /locus_tag="VLS86_03280"
     CDS             1933..3498
                     /locus_tag="VLS86_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HSO49545.1"
                     /translation="MTFLDAPLGERLVASLDTGKPEEIAAAVDEVATRIWAGALSADQ
                     VNEVLEELQRHRQFPHVLGIADAAIAVGCINPSGRRRYCQILIETGALHAAELACREL
                     LELADNDKERGEALGLMGRLSKDRYVTGRRTEDLLAAIAAYRFGYGGGTDRAWHGVNL
                     LALACRAIRDDIAIPDPVDTVALTQTILDEVRREDPQLRTIWDRTTEIEALVALGDDV
                     AIEEAVLAAQQLAASRSVRAFELKSLRRQLIDIWQLEPEHPVLVAIADRQLQLGAAAH
                     VELPDSPGQLEKIFGTALPVGYNHLMRGLQCAESVAKITDASGEPWGTGFLLKGSLIH
                     PDLGEEPILVTNAHVCSPEPGVGKLLPGEAQAVFDVTKSIDGANLVLTGFECLWSSPP
                     ATCDVTFLRFSGSQAALAQPIEVAQVPQPVTEGAYVYVIGHPAGGGLKFSIRGNDLLA
                     YDAELFKVHYTAPTEGGSSGSPVFNQAWQLMAVHHAGDSKMRRLDDPSATYQANEGIT
                     IAAIRKEYELNGR"
     gene            3488..>3712
                     /locus_tag="VLS86_03285"
     CDS             3488..>3712
                     /locus_tag="VLS86_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49546.1"
                     /translation="MGDDTIPAEFLDPEGDGERQDPDLVLEATSATLRKLWSDWMCRR
                     GGGTYEDVVFFGRKVGGVPTPAVDAYRALEQ"
BASE COUNT          650 a         1202 c         1268 g          592 t
ORIGIN      
        1 gatggccgat gttggactct cgccggccgt cggctagaaa cggggcccac tcgttgggag
       61 acagatgaga gccgatatcg gggcgtttgg tgtgtggcga atggtgtcgg ggatcacccc
      121 tgagctggcc gaacaggtgg aggcgctggg gtacggggcg atttggctcg gcggctctcc
      181 gaccggcgat ctcgaagcag tggaggtcgt gctcgacgcc acggagcgga taccggtggc
      241 gaccggaatc gtgaacatgt ggcgagagga cgccgccacc gtggccgagt cctaccgacg
      301 tgtcagcggt cgacatgccg gccggttcct gctcggggtc ggggtcgggc atcgggaggc
      361 gacccaggag taccacaagc catacgacaa gatggtcgac tatctcgacc ggctcgacgc
      421 ggccgccgtg ccccaagagg atgtcgtgct cgccgccctc ggcccccggg ccctcaccct
      481 ggctgcggag agaacctccg gagcccatcc ctacctgacc accccgcgac acaccgatca
      541 ggcacggctg ttgatcgggc gcgacccgct tctcgcccca gagcagaaag tggtgctgga
      601 gaccgatgcc acactggcgc gggccatcgg gcgaccggtg gtcagtcgct atctgacccg
      661 gatcaactac cggagcaacc tgctgcggga aggctgggcc gaatccgacc tcgaggcggg
      721 gggcagcgac cggctggtgg acgcattggt gctacatggc acttcggagg acatcgctgc
      781 cggcctgacc gcccatctcg atgccggggc ggaccatgtc gcgatccagg ttctcggcga
      841 cgacccgctc gacggctatc ggcagctgtc cggagtcctg atcgcggtct gagggggcat
      901 ttcccccctg cgccgggcgc acccgacgcg tacgctcccg tgcagaaagg gggacgcatg
      961 ggccacatca cgttcgtcca cgggatcatg aatcagccgt caccggcccg tcttctcggc
     1021 tcgtggaagc ggcatctcgc cgacggggga gatggtctcg atctcgacgt ctacggggtc
     1081 acgtcatcgg tggtctattg ggccgatgtc atgtacccgg cgaccaccga cgccgggacc
     1141 acccaggaat cgatcgaata cgagggactc gagtcgggag ccttgactca ggtcgacgag
     1201 tcgtacatcg aggaggccgg cggcgaggag aaggccttca tcgaggcgat gatcgccaac
     1261 tatggcctgg accatcccga cgaggccgac acgccagcgg acacctcgag cgccacgcag
     1321 cctgcgggcg aggttgaagc cgaggtccgg ctgacggctg cggcggccct ggaggcggtc
     1381 ccccttccct ggttcatcaa gcggccgatg atgcgggtgt tgctccgcga cgtgcatcac
     1441 tatctgttca acaccagcca ctccccccgc cccggctcca agttcaaggt acgtgacgag
     1501 gtgaggggtc ggttcgtgag tgacctgggg gctgtcgaca acggcccgca cgtggtggtg
     1561 gcgcacagcc tgggcacggt gatcgcctac gactgtctca agcgggtggc cgactgcaag
     1621 aagatcgacc tgctggtgac gctcggaacg ccgctcgggc tctccgaggt tcaggacaag
     1681 ctcaagccgg aatggtccaa ggacgacggg ttccccgacg gcctccctcg ctgggtgaac
     1741 gtggtggacc gtctcgaccc ggtgtgcgcc gccgatccga agatcgccaa cgactaccag
     1801 cacaagaagc aggccgtggt ggtcgacgaa gtggtgtcga acggtggggt gatgcgtcac
     1861 ccacccgaca agtacttccg gcaaccggtg gttcaggcgg cgatcaagga aggtctcggc
     1921 ctgtgagggg aaatgacgtt tctcgacgcc ccgctcggtg agcggctggt cgccagcctc
     1981 gacaccggca agcccgagga gatcgcagcg gccgtggacg aggtggcgac caggatctgg
     2041 gccggtgccc tctccgccga tcaggtcaat gaggtgctgg aagagctgca gcgccaccgg
     2101 caattccccc atgtgctcgg gatcgccgac gccgcgatcg ctgtcggttg catcaaccct
     2161 tccggccgcc gccgctactg ccagatcctg atcgagaccg gcgccctgca cgcagccgag
     2221 ctggcgtgcc gggagctgct cgaactggcc gacaacgaca aggagagggg cgaggcgctc
     2281 ggattgatgg gccgtttgag caaggaccgt tacgtcaccg gtcgcaggac cgaagacctg
     2341 ctcgccgcaa tcgcggccta tcggttcggc tacgggggtg gcaccgatcg agcgtggcac
     2401 ggcgtgaacc tgctggcgct cgcctgtcgg gcaatccgcg acgacatcgc gatccccgac
     2461 ccggtcgaca ccgtggccct cacccagacc atcctcgacg aggtgcggag agaggatccc
     2521 cagctgcgca ccatctggga ccgcaccacc gagatcgagg cattggtggc cctcggtgac
     2581 gacgtcgcca tcgaggaagc ggtgctggcc gcccagcagc tcgctgccag ccgaagtgtc
     2641 cgcgccttcg agctcaagag cctacgtcgc cagctgatcg acatctggca gctggagccc
     2701 gagcacccgg tgctggtggc catcgccgac cgccaactgc agctgggagc ggcggctcac
     2761 gtcgagctgc ccgactcgcc gggccagctg gagaagatct tcgggaccgc tctccccgtc
     2821 gggtacaacc acctgatgag aggtctgcag tgcgcggaat cggtggccaa gatcaccgac
     2881 gcgtcgggtg agccgtgggg caccgggttt ctcctcaagg gaagcctgat tcaccccgac
     2941 ctcggggaag agccgatcct ggtcaccaac gcccacgtgt gcagccccga gcccggagtg
     3001 ggaaagctgc ttccaggtga ggcacaggcg gtgttcgacg tgaccaagtc gatcgacggg
     3061 gcgaatctgg tcctcaccgg gttcgagtgt ctgtggagct cgcccccggc cacctgtgac
     3121 gtcacctttc tccgcttctc cggttctcag gcagccctcg cccagccgat cgaggtggcc
     3181 caggtgcccc agcccgtgac cgaaggggcg tacgtctacg tgatcgggca ccccgcgggc
     3241 ggtgggctca agttctcgat ccggggcaac gacctcctgg cttacgacgc cgagttgttc
     3301 aaggtccact acacggcgcc caccgagggc ggcagctcgg ggagcccggt tttcaatcag
     3361 gcatggcagt tgatggcagt ccatcacgcc ggtgattcga agatgcgtcg actcgacgac
     3421 ccgtcggcca catatcaggc caacgagggg ataacgatcg ccgccataag aaaggagtac
     3481 gagctaaatg ggcgatgaca ccattcctgc agagttcctc gaccccgaag gcgacggaga
     3541 gcgccaggat cccgatctcg tcctcgaggc cacctcggcg acgttgcgca agctgtggag
     3601 cgactggatg tgccggcgtg gtggtggcac ctacgaggat gtcgtcttct ttggccgcaa
     3661 agtgggcggg gttccgaccc ctgcggtcga cgcctaccgg gcgctggagc ag
//
LOCUS       DATITW010000235         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_732547, whole genome shotgun sequence.
ACCESSION   DATITW010000235 DATITW010000000
VERSION     DATITW010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732547"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VLS86_03290"
     CDS             complement(<1..511)
                     /locus_tag="VLS86_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSO49547.1"
                     /translation="MARIILIEDDIEIRRLVADALATEGHDVETAATAMNGLELGIQN
                     NPDLIIMDLGLPDLDGNELLRMVRAISSVPIMVITARGADEAVVRTLDAGADDYLIKP
                     FSVAQLLARVRAILRRGGLAAGGDRMTVGGLVIDISAREAVLDGVALELSPKEFDLLR
                     SLAERQGEVV"
     gene            614..1129
                     /locus_tag="VLS86_03295"
     CDS             614..1129
                     /locus_tag="VLS86_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49548.1"
                     /translation="MNRFGLIAIWVAATALTTVLAWQIVAAAGEQVSPGPLIEAPIAA
                     PDTSTTVVSTTLPTTSSTSALPTSSSTQPGSGSTTPNTDPGSTTPTTSASWQVKTIPT
                     DGGTVVVSYRPGEVVLDGATPAPGFTVDVDKGGPPEVRVEFESESTKVEVRVQWDGKL
                     VIDLQADSDED"
     gene            complement(1130..1564)
                     /locus_tag="VLS86_03300"
     CDS             complement(1130..1564)
                     /locus_tag="VLS86_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HSO49549.1"
                     /translation="MVTKKEKLDKLGSVPLFSGLGQKDLEQILDRMKETFHVAGDHVI
                     DEGRAGLGFHLILEGEASVIRNGRTAARLGPGEFFGEMALVDDGPRSATVVASTDLTA
                     VVLSKWEFRPLLKDHPEMAWKLIEHLVARVREEQSGRDALVC"
     gene            1641..>2270
                     /locus_tag="VLS86_03305"
     CDS             1641..>2270
                     /locus_tag="VLS86_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49550.1"
                     /translation="MVITESPPETVPNRSGRRLPLILGLLIAGSTFVGIGLLADPGPT
                     REQQMTRSSQSQQTISTWVETYNSGDIETWIALYADDAVVNGTLLKLDPGYIRGVQLM
                     NIEWNERLDLSNCSPADLDVVRCNYRRSNDMLARARITASGVIEFGFEESGSIRRSDL
                     TIVNPEVTTFEKALATWIESEHPEIDLETRFGQMLLVETPADLSPLADEF"
BASE COUNT          430 a          728 c          696 g          416 t
ORIGIN      
        1 tgaccacttc gccctgccgt tccgcgaggg accgcagcag gtcgaattcc ttcgggctca
       61 gctcgagagc tactccgtcg agcactgcct cccgagccga gatgtcgatg accaggccac
      121 ccacagtcat tcgatcaccg ccggcagcca ggccgcctcg tcgcaagatg gccctaacac
      181 gggcgagaag ctgggcgaca gagaagggct tgatgagata gtcgtcggcg ccggcgtcga
      241 gggtccggac cacggcctca tcggcgccgc gggcggtgat caccatgatc gggaccgagc
      301 tgatcgcccg aaccatgcgc aacagctcgt tgccgtcgag atcgggaaga ccgaggtcca
      361 tgatgatcag gtccgggttg ttctggatgc ctagctccag cccgttcatc gcggttgccg
      421 cggtctcgac atcgtgacct tccgtcgcca gtgcgtcggc cacgagtcgg cggatctcta
      481 tgtcgtcctc gatgaggatg attcgcgcca tggcgggcag gctatcggtg gtgatgggtg
      541 taggccggag ccttgagaaa accttgatag tcctttgatt cggtagctag cggcttgctg
      601 ggagaattca gaggtgaacc gattcggcct catcgcgatc tgggtggcgg caaccgcact
      661 caccaccgtc cttgcgtggc agatcgtcgc cgccgcaggg gaacaggtca gccctggccc
      721 gttgatcgag gcacccattg ccgccccgga caccagcacc acggtggtgt cgacgaccct
      781 gcccacgaca tcctcaacgt cggctctgcc cacctcctcg tccacccaac ccggctccgg
      841 atcgaccaca cccaacaccg accccgggtc gacaacgcct accaccagtg caagctggca
      901 agtgaagacg atccccaccg acggagggac cgtggtggtc agctatcggc cgggcgaagt
      961 cgttctcgac ggtgcgactc ccgcgcccgg cttcacggtc gacgtcgaca agggaggacc
     1021 gcctgaggtg cgggtcgaat tcgaaagcga gtcgaccaag gtggaggttc gggtccagtg
     1081 ggacggcaag ctggtcatcg atctccaagc cgacagcgac gaggactgat caacagacga
     1141 gggcgtctct tcccgactgc tcctcgcgaa cgcgtgccac caggtgctcg atgagtttcc
     1201 aggccatttc gggatggtcc ttgagcagag gccggaactc ccacttggag aggacaaccg
     1261 ccgtcagatc tgtgctcgcg accacggttg cggatcgggg accgtcatcg accagtgcca
     1321 tctcaccgaa gaactccccc ggtccgagcc gtgctgcagt gcgcccattt cgaatgacac
     1381 tcgcctcgcc ctcgaggatc aggtgaaacc cgagtccggc ccttccctcg tcgatcacat
     1441 gatcaccggc gacatggaac gtttctttca tccggtcgag gatctgctcc aggtccttct
     1501 ggccgagacc gctgaacaga gggacggacc caagcttgtc gagcttctcc ttcttggtga
     1561 ccaaggcatt cctccacgtg gtgatcggtc accggatcat atcggtcgga gcggtgggaa
     1621 agcgtctgta gccttctggg atggtcatca ccgagagccc tccggagacc gttcccaacc
     1681 gatccggtcg ccgtctcccg ctcatcttgg ggctcctcat cgccggttcg acgtttgtcg
     1741 gcatcggcct cctggccgac cccggcccga cgcgagagca acagatgacc agatccagtc
     1801 agtcgcagca gacgatctcg acatgggtcg agacctacaa ctcgggcgac atcgagacat
     1861 ggatcgcgct ttacgccgac gacgcggtgg tcaacggaac cctgctcaaa ctcgacccgg
     1921 ggtatatccg gggggtccag ttgatgaata tcgaatggaa cgagaggttg gacctgtcga
     1981 attgttcccc ggccgacctc gacgtggttc gttgcaacta ccggaggagc aacgacatgc
     2041 tcgcccgagc caggatcacc gcgtccggtg tcatcgagtt cgggttcgag gagtcgggtt
     2101 cgatccgtcg ctccgatctg acaatcgtca acccggaggt gaccacattc gagaaggcgc
     2161 tggcgacctg gatcgagagc gagcatcccg agatcgacct ggagacccgt ttcgggcaga
     2221 tgttgctggt cgagactcct gccgacctca gtccattggc agacgagttt
//
LOCUS       DATITW010000236         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_11571, whole genome shotgun sequence.
ACCESSION   DATITW010000236 DATITW010000000
VERSION     DATITW010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11571"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VLS86_03310"
     CDS             <1..663
                     /locus_tag="VLS86_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HSO49551.1"
                     /translation="LIEEYMPGAEVALEGIVRDGELTTLALFDKPDAAEGPFFPETLL
                     ITPSALSPQRQAECRRVAGAALRGLGISHGPVHVEMKVDDGQVRVIEVAARSIGGLCS
                     KSLSFGLMGTTLEALILRNAVGMDKPELRREPVASGVLMIPIPREGTFVEIRGLESIR
                     EIPHISAIDITAIPGNVIEPPPEGDRYLGFVFARAESPVLVEGALREAMTTMEVVVDN
                     GS"
     gene            769..1389
                     /locus_tag="VLS86_03315"
     CDS             769..1389
                     /locus_tag="VLS86_03315"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HSO49552.1"
                     /translation="MSTILLFTGGGLPEGNLTEELPTPDLVVAADSGYDLALHLGYRV
                     DVLVGDMDSIAAVDIPDQVLVERHPPDKDQTDLDLAMEFALGKHPRRVVVIGGTGGRF
                     DHEMATSGLLCSDRWARIDEIDWVSSRGRAHVVRGRRIVHGDVGTTLSLIPFGGSARV
                     TTKGLEWDLSGDDLAPGSTWGVSNVLRAPVADIRVESGCVLVVLPT"
     gene            complement(1391..>2281)
                     /locus_tag="VLS86_03320"
     CDS             complement(1391..>2281)
                     /locus_tag="VLS86_03320"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HSO49553.1"
                     /translation="SLAEVEVWEGEGQVPRSTLIDRLAACHGWLSMLTDTIDAELLDA
                     APDLTVISQMSVGVDNIDVGACRERGVRLGHTPDVLTETVADTAFALMAAAVRRLPQG
                     EALVKSGAWGPWDPWHSLGGDLYRAQLGIIGMGRIGQAIARRARGFDMEVRYTSPSPK
                     DVPDSSQVDLDDLLAESYLVVVSAPLTSETKSMIGARELELMRSDAILVNIARGPLVD
                     TAALVDALARSSIGGAALDVTDPEPLPPDHPLLEFPNCLVVPHIGSASVRARRAMAQL
                     AVDNLVAALRGEPMPASLLD"
BASE COUNT          405 a          719 c          771 g          386 t
ORIGIN      
        1 ctcatcgagg agtacatgcc gggagccgag gtggcactgg aggggatagt ccgggacggg
       61 gagctgacca ccctcgctct attcgacaag cccgatgcgg ccgaggggcc cttcttcccc
      121 gagactctgc tcatcactcc gtcggctctc tctccccagc ggcaggccga atgccggaga
      181 gtggcgggag ccgccctgcg cggcctcggg atcagccacg ggccggtcca cgtcgagatg
      241 aaggtggacg atggccaggt tcgggtcatc gaggtggcgg cacgatcgat cggcgggctg
      301 tgctcgaaga gcctcagctt cgggctgatg ggaaccaccc tggaggccct gatcctgcgc
      361 aatgcggtag gcatggacaa gcccgagttg cgacgtgagc ccgtcgccag tggagtcctc
      421 atgatcccga ttccccggga aggaaccttc gtcgagatca ggggcctgga atcgatccgg
      481 gagatccccc acatctccgc gatcgacatc accgcgatcc ccggcaacgt catcgagccg
      541 ccacccgaag gtgaccgata tctcgggttc gtgttcgcca gggcggagtc gcccgtcctc
      601 gtcgaggggg cgctgcggga ggcaatgacc acaatggaag tcgtcgtcga caatgggtca
      661 tgactcgatc ccggtcgatg tgcagagccg acgggcgttt cagaccctca acctcaactt
      721 gaaggtgagt tcttaccccc atttcgcgcg taagacgatt ttgtcattat gagcaccatt
      781 ctcctcttca ccggtggtgg tctccccgag gggaatctga ccgaggagct gcccacccct
      841 gatctggtgg tggcggcgga cagcggatac gacctggccc tccatctcgg gtatcgggtc
      901 gacgtgttgg tcggggacat ggactcgatt gctgccgtcg acatccccga tcaggtgctg
      961 gtcgagcggc atccgcccga caaggaccag accgatctcg atctggcaat ggagttcgcc
     1021 ctgggcaagc acccgcgccg ggtcgtggtg atcgggggca ccggtggccg gttcgatcac
     1081 gagatggcga cctccggtct gctctgctcc gatcgatggg caaggatcga cgagatcgat
     1141 tgggtctcct cgagaggaag ggctcatgtg gtgcgaggca ggcggatcgt ccatggcgat
     1201 gtggggacca cgttgagtct cattcccttc ggcgggtcgg cgagagtgac gaccaagggt
     1261 ctggaatggg acctgagtgg cgatgaccta gcgccgggat cgacctgggg agtgagcaac
     1321 gtgctgcggg ccccggtggc cgacatccgg gtcgagtcgg gatgtgtgct ggtagtgctc
     1381 cccacctgag tcagtcgaga agcgaagccg gcatcggctc gccccgaagc gctgcgacca
     1441 ggttgtccac agccagctgg gccatggccc tcctcgcccg caccgaggca ctcccgatgt
     1501 gggggaccac caggcagttg gggaactcga gcagcgggtg gtctgggggc aggggctcgg
     1561 ggtcggtgac atcgagtgcc gcacccccga tcgagctccg ggcgagggca tcgaccaggg
     1621 ctgcggtgtc caccaaaggg ccgcgggcga tgttgaccag aatggcatcg ctgcgcatca
     1681 gttccagctc tctggctccg atcatcgact tcgtctccga ggtcaagggc gcggagacaa
     1741 cgaccagata ggactccgca agcaggtcgt cgaggtccac ctgagacgag tcgggaacat
     1801 ccttcggaga gggagacgtg tagcgaacct ccatgtcgaa gccgcgggca cgacgggcta
     1861 ttgcctggcc gatccgaccc atcccgatga tgccgagctg cgcccgatac aggtctcccc
     1921 cgagagaatg ccacgggtcc caaggccccc aggctcctga cttcaccagg gcctcgcctt
     1981 ggggcaatcg ccggacggcg gcagccatca gggcgaatgc ggtgtccgcc acggtctcgg
     2041 tcagcacatc gggtgtgtgg ccgagccgga ccccgcgctc ccggcaggcg ccgacatcga
     2101 tgttgtccac ccccaccgac atctgcgaga tgaccgtgag atcgggagca gcgtcgagca
     2161 attcggcgtc gatggtgtcg gtcagcatcg acaaccaccc atggcatgcc gccagccgat
     2221 cgatgagcgt cgaccgggga acctgcccct caccctccca cacctcgact tcggcgaggg
     2281 a
//
LOCUS       DATITW010000237         1998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_11579, whole genome shotgun sequence.
ACCESSION   DATITW010000237 DATITW010000000
VERSION     DATITW010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11579"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1411)
                     /gene="nhaA"
                     /locus_tag="VLS86_03325"
     CDS             complement(56..1411)
                     /gene="nhaA"
                     /locus_tag="VLS86_03325"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HSO49554.1"
                     /translation="MPGNETHVTWARSERFVPRAFIRPFMRFSNREASSGLVLVAAAA
                     IAVVWANLWPESYESLLSTTLRLDFGPLHLEEDIHHRINDGLMAVFFFVVGLEIKREL
                     VVGELRDRRAAALPVIAALGGMAAPALVYLAMAGASGEPGRGWGIPMATDIAFAIGIL
                     SLVGKKVPVGAKLFLLTLAIADDIGAILVIAVFYTAELSMGWLALAGGGLAAVVLAQR
                     VGIRSSTLYAPLAILVWFATLESGVHATLAGVALGLLTPARPYYDYDEFRAKGRELMT
                     GLVPEDDDVDERELADHTLLTISEISRESVSPLARIEHRLGNWSSFVVVPLFALANAG
                     VNFQGSSVDEALLSPVGLGVALGLIVGKIVGITGFTLAAVRLKAGRLPEGVTVRHVLG
                     VATVAGIGFTVSLFIANLAFSDPDLIEDAKVGVFAASIVAALLGWLVLRAGPNAKGDS
                     G"
     gene            complement(1413..>1998)
                     /locus_tag="VLS86_03330"
     CDS             complement(1413..>1998)
                     /locus_tag="VLS86_03330"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HSO49555.1"
                     /translation="AECTRVGSILELEIGVVGGKEDATDHEGIDKAKLYTTPEDMSRT
                     VEVLGSPGETMYLLAAVFGDGHGVYKPGAVQLRPSILRDGQAAVEARFGPAGRHYLVF
                     HGGSGSTLDEIRETIGYGVVKMNVDTDTQYAFTRPIADHMFSNYSGVLKVDGEVGDKS
                     VYDPRSYLKKAEAGMAARVTRACSDLMSSGKSLR"
BASE COUNT          406 a          640 c          619 g          333 t
ORIGIN      
        1 gagagcaggt gcagggtccc gtcgcaatcc acacatggaa tggccaccgg cggtctcacc
       61 cggaatcccc tttggcgttc gggcctgccc tcagaaccaa ccacccgagc aatgccgcca
      121 cgatcgaagc agcgaaaacc ccgaccttcg catcctcgat cagatcggga tcggagaatg
      181 cgaggttggc gatgaatagc gaaaccgtaa agccgatacc ggcaacggtg gctacgccga
      241 gaacatgacg gacggttacg ccctctggca agcgccctgc tttgagcctg actgccgcca
      301 gcgtgaatcc ggtgatcccg acgatcttcc cgacgatgag acccagcgcc actccgagtc
      361 ccaccgggct cagcagggct tcgtccacgg agcttccctg gaaattgacg ccggcgttgg
      421 ctaaagcgaa cagcgggacc acgacgaagg atgaccaatt cccgagtcga tgctcgatcc
      481 gggccaatgg tgagaccgat tcgcgcgaga tctcagagat ggtgaggagg gtatggtcgg
      541 ctagttcgcg ctcgtcgacg tcgtcgtcct cggggaccaa acctgtcatc agctcacggc
      601 ccttggcgcg aaactcgtca tagtcgtagt agggacgggc cggggtgagg aggcccaggg
      661 ccactccggc gagggtggcg tgaaccccgg attcgagggt ggcaaaccag accagaatcg
      721 ccagcggggc atacagggtg ctggagcgga tcccgactct ctgggcgaga actacggcag
      781 cgagaccgcc cccggcaagg gcgagccatc ccatcgagag ttcggcggta tagaagacgg
      841 cgatgacgag gatcgccccg atgtcgtctg caatcgccag ggtgagcagg aacagcttgg
      901 ccccgaccgg caccttctta ccgaccaggg agaggatccc gatggcgaag gcgatgtcgg
      961 tggccatcgg gattccccaa ccccgtcccg gttccccgga ggcgccggcc attgccagat
     1021 agaccagggc cggggccgcc attccaccga gtgcggcgat cacggggaga gccgcggccc
     1081 tgcgatcgcg gagttctcca accaccagtt ctcgcttgat ttcgagccca acgacgaaga
     1141 agaagacggc cataagtccg tcattgatcc gatgatggat gtcctcttcc agatggaggg
     1201 gcccgaagtc gagccggagg gtggtcgata gcaacgactc atacgactcc ggccacaggt
     1261 tggcccacac caccgcgatc gcggcggctg caaccaggac gagaccggac gacgcctccc
     1321 gattgctgaa tcgcatgaat gggcgaatga acgccctggg gacgaagcgc tccgaccgcg
     1381 cccaagttac gtgggtctcg ttgccgggca cgtcagcgga ggcttttacc gctcgacatg
     1441 aggtcggaac aggcccgcgt gacgcgtgcg gccatccccg cctcggcctt cttcaaatag
     1501 gagcgagggt cgtagacgga cttgtcaccc acctcgccgt caaccttcag cacgccggag
     1561 tagttggaaa acatgtgatc ggcaattggc cgggtaaagg cgtactgagt gtcggtgtcg
     1621 acgttcatct ttacgacgcc ataaccgatc gtctcccgga tctcgtcgag ggtcgatccc
     1681 gacccaccgt gaaagacgag atagtgacgg ccggccggcc cgaatctggc ttcgaccgca
     1741 gcctggccat cacgaaggat gcttggccgc aactggaccg cgccaggctt atagacgcca
     1801 tgcccatctc cgaatactgc cgccagcagg tacatcgtct cacccggcga ccccaggact
     1861 tccacggtgc gactcatgtc ctccggggtc gtgtagagct tggctttgtc gatcccttcg
     1921 tgatccgtgg catcctcttt gccgccgacc actccgatct cgagttcaag gatgctccct
     1981 acccgcgtgc actccgcc
//
LOCUS       DATITW010000238         1832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_251708, whole genome shotgun sequence.
ACCESSION   DATITW010000238 DATITW010000000
VERSION     DATITW010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251708"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VLS86_03335"
     CDS             <1..1072
                     /locus_tag="VLS86_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HSO49556.1"
                     /translation="AATLDLTAYPGLEVVGVQGERGVALGVDEPMRSGYLISVRGEAE
                     VIHGFAADNDLERKPGKTGGALINRSIGVVEVVIPPRSDLIGRKVFPGMVTESGDFVI
                     LAVQRAGENMDREATVLTVGDTMLLQGTWDALDRQIDGHQDLLVVDSPGLVRRQAVAL
                     GPRAWWAISILVGMVVLLATNAVPAVEAALLAAGAMILTRTVTMLQAYRSISWTTVIL
                     VAGMIPLSTAVTSSGAADDIADLLIDLVGDLGPYALLSGLFVLTAVFGQLISNMATAL
                     IVIPIAVTAAVDLGVSVRPVLMSITVAAAAAFLTPVATPANLMVMGPGGYQFGDYWKL
                     GLPLLLVFMTAAVFLVPVFWQF"
     gene            complement(1119..>1832)
                     /locus_tag="VLS86_03340"
     CDS             complement(1119..>1832)
                     /locus_tag="VLS86_03340"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HSO49557.1"
                     /translation="IGSLIGARYFTMPAQILDGVPLFEALNPIRGNFSIMGGFAGGIL
                     AGGWRMRQVGLPVLATLDVSSFGLALGTIVGRLGDLAIVEHLGRATDLAWGYGIKPGY
                     DVAPAHDALECTESVAVDGLCGVYHHVAVYDLIGAVILLGALYLVYRRFRLHYGQLFF
                     LWVSWYGLQRFVLDALRFGSESGDATIGIFTWNQVSGLAAGVAGIAAFVWVGSVQPVV
                     SEEEDARLAGAAPVPTPLR"
BASE COUNT          307 a          609 c          599 g          317 t
ORIGIN      
        1 ggcggcgaca ctggatctca ccgcatatcc gggcctggaa gtggttgggg tccagggtga
       61 gcgaggtgtc gccctcgggg tcgacgagcc gatgagaagt ggatacttga tcagcgttcg
      121 cggtgaggcc gaggtgatcc acggtttcgc ggccgacaac gatctcgaga gaaagccggg
      181 caagaccggc ggagcactca tcaaccgcag tatcggtgtg gtcgaggtgg tcatcccgcc
      241 ccggtcggac ttgatcggga gaaaggtgtt ccccgggatg gtcaccgaga gcggcgactt
      301 cgtcatcctc gccgtgcagc gggcgggcga gaacatggac cgggaagcga ccgtcctgac
      361 tgtcggtgac accatgctgc tccaggggac gtgggacgct ctcgaccgac agatcgacgg
      421 ccaccaggac ctcctggtgg tcgactcccc cgggctggtc cggcgacagg cggtggctct
      481 cggcccccgg gcctggtggg ccatttcgat cctggtggga atggtcgtgc tgctggcgac
      541 caacgcagtg ccggcggtgg aagctgcact gctggcggcg ggggcgatga tcctgacgcg
      601 aacggtcacc atgctgcagg cttaccggtc gatctcatgg accaccgtga tcctcgtcgc
      661 cgggatgatc ccgctgtcca cggcggtgac cagcagtggt gccgccgacg acattgccga
      721 cctgctgatc gacctcgtgg gtgacctcgg gccctacgcc ctcctctcgg gcctcttcgt
      781 gctcacggcg gtcttcgggc aattgatctc caacatggcc accgccctga tcgtgatccc
      841 gattgcggtc accgccgcgg tcgatctcgg tgtgtccgtg cgcccggtgc tgatgtcgat
      901 cacggtcgct gcggctgcgg ccttcctcac tccggtggcc acccccgcca atctgatggt
      961 catgggcccg ggtggttacc agttcggtga ctattggaag ctcggtctgc ccctgctcct
     1021 ggtgttcatg actgccgctg tgttcctggt gccggtgttc tggcagttct gaagcggtgc
     1081 cagggagatg atcgagtgtc tccctccgtt ttgcaggttc agcgaagagg ggtcgggacc
     1141 ggggcggctc ccgccaacct ggcgtcctcc tcctcggaga ccaccggctg caccgacccc
     1201 acccacacga atgccgcgat gccggcaaca ccggcggcca gcccgctgac ctggttccag
     1261 gtgaagatcc cgatcgtggc gtcgccactc tccgacccga atcggagggc gtccaggacg
     1321 aatctctgca acccgtacca gctcacccac aggaagaaga gctgcccgta gtggagcctg
     1381 aatcggcgat acaccaggta gagcgctccg agcaggatga cggcgccgat caggtcatag
     1441 accgccacgt ggtgatagac cccgcacagc ccatcgaccg cgacactctc ggtgcattcc
     1501 agcgcatcgt gcgccggcgc cacgtcatag cctggcttga tcccataacc ccaggcgagg
     1561 tccgtcgccc gccccagatg ctcgacgatg gcgaggtcgc cgagccgccc cacgatcgtg
     1621 cccaacgcca acccgaacga cgacacgtcg agcgtcgcca gcaccggcaa cccgacctgt
     1681 cgcattctcc agcctccggc gaggatgccc ccggcaaagc ctcccatgat cgagaagttg
     1741 ccccgaatcg ggttcaatgc ctcgaagagg gggacgccat cgaggatctg ggcgggcatc
     1801 gtgaagtacc gggccccgat cagggagccg at
//
LOCUS       DATITW010000239         1677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_331887, whole genome shotgun sequence.
ACCESSION   DATITW010000239 DATITW010000000
VERSION     DATITW010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331887"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VLS86_03345"
     CDS             complement(<1..218)
                     /locus_tag="VLS86_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49558.1"
                     /translation="MTEDSQRAQGEPDPQVEVESDPTVVYVPIQDRADHPMTREEDRS
                     VEGFTGYALVIERGPQTGMTYVLPPGKTT"
     gene            complement(264..644)
                     /gene="gcvH"
                     /locus_tag="VLS86_03350"
     CDS             complement(264..644)
                     /gene="gcvH"
                     /locus_tag="VLS86_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HSO49559.1"
                     /translation="MQVPDDLLYTEEHEWIRRLSDSTVRIGIHDYAQDQLGDVVFVEL
                     PEVGASVGKDDILVEVESTKSVGEVYAPFSGRISAVNVALADAPELVNQSPYEQAWFV
                     EIEVTDGVDTTGLLDASAYRELTE"
     gene            complement(644..1240)
                     /locus_tag="VLS86_03355"
     CDS             complement(644..1240)
                     /locus_tag="VLS86_03355"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSO49560.1"
                     /translation="MTEAVAANRVLTIPNLVSFVRLLAVGLFWWVLLVQDDIALAAWL
                     IFAIGWTDWIDGYLARRLNQVRDLGKVLDPVADRLMIASAVIGGLIVGVVPVVVGVLL
                     IVREMVVGLLALYLAARGGGTIEVRYYGKLATFLLYGAIPGFYLAAAGFLEWLLLPAA
                     WVAAVIGLALYWYVALLYLGDTRRRLAALKSPTGPEEV"
     gene            complement(1274..>1677)
                     /locus_tag="VLS86_03360"
     CDS             complement(1274..>1677)
                     /locus_tag="VLS86_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49561.1"
                     /translation="DFDTVVDLAFTVDQSGARQYDMYVLGWTLGNPALPEYHRWLFAG
                     DGAANSTGYANADFEASLDRFERAAVPDEARDALWDMELAVARDLPYLVLYHPEITEA
                     YRSDRLDFGIRDVLGGIQGRLGGLADLTTNP"
BASE COUNT          321 a          548 c          533 g          275 t
ORIGIN      
        1 gtcgttttgc caggcgggag gacgtaggtc atcccggttt gaggcccgcg ctcgatgacc
       61 agcgcataac cggtgaatcc ctcgacgctg cggtcctcct cgcgggtcat ggggtggtcg
      121 gcgcgatcct ggatcgggac gtagaccacc gtagggtcgc tctccacctc aacttgaggg
      181 tcgggctcac cctgggcgcg ctggctgtcc tcggtcatga gaccagatga tagggagtgc
      241 ccggccttgc gccgagcctc gccttactcg gtcagttcgc ggtacgcgct cgcatcgaga
      301 aggccggtgg tatccacacc gtcggtgact tcgatctcaa cgaaccaggc ttgctcatag
      361 ggactctgat tcaccaattc gggggcatct gccagcgcga cattgaccgc gctgattcgg
      421 ccggagaagg gggcgtacac ctcccccacc gacttggtgc tctcgacctc gaccaggatg
      481 tcgtccttgc ccaccgatgc ccccacctcg ggcaactcga cgaacaccac gtcccccaac
      541 tgatcctggg cgtagtcgtg gatcccgatc cgcacggtgg agtcgctcag acgtcggatc
      601 cactcgtgct cttctgtgta gagaagatca tcgggtacct gcatcaaacc tcctcagggc
      661 cggtgggcga ctttaacgct gcgagacgtc gccgggtgtc tcccaaatag agcagggcaa
      721 cgtaccagta gagggcgaga ccaatcacgg cggcgaccca ggccgcaggc aatagcagcc
      781 actcgaggaa tccagccgcg gccaggtaga acccggggat cgctccgtag aggagaaatg
      841 tcgccaattt gccgtagtac cgcacctcga tcgtgccccc gcccctggcc gccagataga
      901 gagccagcag ccccaccacc atctcgcgga cgatgagcag cacgccgaca acgaccggga
      961 ccacgccgac tatgaggccg ccgatgaccg cggaggcaat catcagacga tcagcgaccg
     1021 ggtcgagcac ctttcccagg tcgcggacct gattcagacg gcgggcgaga tagccgtcga
     1081 tccagtcggt ccagccgatg gcgaatatca gccaggccgc cagcgcaatg tcgtcctgga
     1141 cgaggaggac ccaccagaac aggccgacag ccaacaggcg aacaaaggac accaggttgg
     1201 ggatggtgag gactcggttg gccgcgactg cctcggtcac cggattcagg ctaacccggc
     1261 cggcccccga gccttagggg ttagtggtga ggtcggcgag accgccgagt ctgccctgta
     1321 tcccacccag gacatccctg atcccgaagt cgagccgatc cgagcggtag gcctcggtga
     1381 tctcggggtg atagaggacc agatagggca gatctcgtgc aacggccagc tccatgtccc
     1441 acaacgcatc cctcgcctcg tcgggcactg ccgcccgctc gaagcggtcg agactggctt
     1501 cgaagtcggc gttggcgtac ccggtcgagt tggccgcccc gtcgccggcg aagagccatc
     1561 ggtggtattc ggggagggcc gggttgccca gtgtccaccc gaggacgtac atgtcgtact
     1621 ggcgggcgcc ggactgatcc accgtgaacg ccaggtcgac gacagtgtcg aagtcgg
//
LOCUS       DATITW010000240         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_692591, whole genome shotgun sequence.
ACCESSION   DATITW010000240 DATITW010000000
VERSION     DATITW010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692591"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VLS86_03365"
     CDS             complement(<1..211)
                     /locus_tag="VLS86_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010596607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49562.1"
                     /translation="MTDPPVPARPAATVMLLRDAEPGVEVLMVSRPARGFFGGLTVFP
                     GGAVDPEDESALAAEVVISDDPDRVY"
     gene            259..1605
                     /gene="selA"
                     /locus_tag="VLS86_03370"
     CDS             259..1605
                     /gene="selA"
                     /locus_tag="VLS86_03370"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HSO49563.1"
                     /translation="MSQPANPYRELPSVDEVLSTLAGDGLPRPLLVDTARSAIALARA
                     AIGRGESPDVNRLAADLARGLRRSATQRVVNATGVLLHTNLGRAPLSPRAAEAARLAA
                     VSYTNLELDLSTGDRGARGSYVRQLLRSLTGAEDAIVVNNNAAAVLLSLAATASGRSV
                     PVSRGELIEIGGSYRLPLVMEAGGARLIEVGTTNRTRADDFRTALQIHDCGAVLRVHP
                     SNYRVEGFVAEASLADLVELAHNHGVPLIHDLGSGLLDANAPWLGTRPPSWLAGEPGA
                     RQSIEAGADLVTFSGDKLIGGPQAGIVLGTETAVDRLRRHPLTRALRVDAMTDAALGA
                     TLEAFAGGGAREIPFWQMATLDQSDLTPRVEALAARIGGTVFVGESVIGAGSLPGVTI
                     PTPQIALEGQDHLHGELLATAQPILSRRQGSDLVIDIRAVVPEDDDVIADMVAMCR"
     gene            1596..>2329
                     /gene="selB"
                     /locus_tag="VLS86_03375"
     CDS             1596..>2329
                     /gene="selB"
                     /locus_tag="VLS86_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HSO49564.1"
                     /translation="MPLIGTAGHVDHGKSTLIETLTGRDPDRGAEEKRRGLTIDLGFA
                     WTTLQPGLEVSFVDVPGHERYLKNMLAGIEVVDAALFVVAADEGWMPQSEEHLAVLDL
                     LGIDTGVVALTKVDLVDDDTVDLAIIEISEQLAGTSMESAEVIPVSARTGLGIDRLRS
                     ALADLVSGISAGGDRPRLWIDRAFTVPGAGTVITGTLIEGPIQVKDTVEILPGHRTAR
                     IRALQSHETEVDRAEPGRRLALNLSGV"
BASE COUNT          366 a          816 c          793 g          354 t
ORIGIN      
        1 ggtacacgcg atcggggtcg tcactgatga ccacctcggc ggccagggcc gactcgtcct
       61 ccgggtccac cgctccaccg gggaacacgg tcaaccctcc gaagaatccc ctcgccggcc
      121 ggctcaccat gaggacctcg acccccggtt cggcgtcgcg gagaagcatc actgtcgccg
      181 ccggacgcgc cggcacgggt gggtcggtca ctccggcatc ctatgaaacg agccggatca
      241 acatggcaca cttcgcaggt gtcccagccc gccaacccgt atcgtgagct tccctcagtc
      301 gacgaggtcc tcagcaccct ggccggagac ggtcttcccc gccccctcct ggtcgacacc
      361 gcacggtcgg cgattgcgct ggctcgcgcc gcgatcgggc gaggagagtc accggacgtc
      421 aatcgtctcg ccgccgacct ggcgcgaggg ctccgtcgct cggcaacaca acgtgtggtg
      481 aacgcgaccg gcgtcctcct ccacaccaat ctcggacgtg ccccgctctc gccgcgcgca
      541 gccgaggcgg cgcgcctggc cgcggtctcc tacacgaatc tcgagctcga cctgtccacc
      601 ggtgaccgcg gcgcccgggg cagctacgtg cgccaactgt tgcggtcgct gaccggcgcc
      661 gaggatgcca tcgtcgtcaa caacaacgcc gctgcggtgc tcctctccct ggccgcgacg
      721 gcttcgggca gatcggtgcc ggtgtcccgc ggtgagctga tcgagatcgg gggctcatat
      781 cgcctccccc tggtgatgga ggcaggtggg gcgaggctga tcgaggtggg gaccaccaat
      841 cgaaccaggg ccgacgactt caggaccgcg cttcagatcc acgactgcgg cgccgtgctc
      901 agggtccacc cctcgaacta ccgggtcgag ggatttgtcg ccgaggcgtc actggccgac
      961 ctcgtcgagc tggcacataa ccacggcgtg cccctgatcc acgacctcgg atcgggtttg
     1021 ctcgacgcca acgctccctg gctcggaacc cggcccccgt catggctggc cggggagccg
     1081 ggggcccgcc agtcgatcga agccggcgca gacctggtga ccttcagtgg cgacaagctg
     1141 atcggaggcc cccaggccgg gatcgtgctc ggcacggaaa cggccgtgga ccggcttcgt
     1201 cgtcacccgc tgacccgagc gttgcgggtc gacgcgatga ccgacgctgc tctcggggcc
     1261 acgctggagg ctttcgccgg aggtggggcg cgggagatcc cgttctggca gatggccaca
     1321 ctcgatcaga gcgatctcac tccccgggtc gaggcgctgg cggcgcgcat cgggggcacg
     1381 gtcttcgtcg gggagtcggt catcggcgcc ggaagcctcc ccggagtgac gatccccacc
     1441 ccccagatcg cactcgaggg ccaggatcac ctccacggcg agctgctggc cacggcccag
     1501 ccgatcctgt cgagacgcca gggctcggac ctggtgatcg acatacgagc ggtcgtcccc
     1561 gaggacgacg acgtcatcgc cgacatggtg gccatgtgcc gctgatcggc accgccggtc
     1621 acgtcgacca tggcaagtcg acactgatcg agaccctgac agggagggac cccgatcgcg
     1681 gggcggagga gaagcgacgc ggcctgacca tcgacctcgg gtttgcctgg accacgctcc
     1741 agcccggcct ggaggtctca ttcgtggacg tccccggcca tgagcgctac ctgaagaaca
     1801 tgctggccgg gatcgaggtg gtcgacgccg ccctgttcgt ggttgcggcc gacgaggggt
     1861 ggatgccgca gtccgaggag catctcgccg tcctcgacct gttggggatc gacaccgggg
     1921 tggtggcact caccaaggtc gatctcgtcg acgacgacac cgtcgatctg gcgatcatcg
     1981 agatctccga gcagctcgcc gggaccagca tggagtcggc ggaagtgatc ccggtgtcgg
     2041 cacgaaccgg gctcggcatc gaccggttgc ggtcggcact ggccgacctg gtgtccggga
     2101 tctcagcggg cggcgaccgg ccccgtctct ggatcgaccg ggcgttcacg gtgcccggcg
     2161 ccggcaccgt gatcaccggg accctgatcg agggcccgat tcaggtcaag gacacggtgg
     2221 agatcctccc cggtcaccgg acggcgcgga ttcgggcctt gcagagccac gagaccgagg
     2281 tggacagagc cgagcctggc cggcgtctcg ccctgaatct gagtggggt
//
LOCUS       DATITW010000241         1955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_372038, whole genome shotgun sequence.
ACCESSION   DATITW010000241 DATITW010000000
VERSION     DATITW010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372038"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..774
                     /locus_tag="VLS86_03380"
                     /pseudo
     CDS             4..774
                     /locus_tag="VLS86_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
     gene            827..1405
                     /locus_tag="VLS86_03385"
     CDS             827..1405
                     /locus_tag="VLS86_03385"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HSO49565.1"
                     /translation="MKLLFIGPPGAGKGTQASRVAERLGIPHISTGEMFRHHVSSGTD
                     LGRRVDAIMKSGEYVPDEITVSMLRERLAEPDARAGFILDGFPRTAGQVEALDGLIGA
                     DGLDHVVVLEADEDELVERLLARGRADDTDATVRNRFAVYQDQTAPLLDVYRGRGSLV
                     HVSGMGPMDEVTERILSALAPPEPGRVEEGTP"
     gene            1402..>1955
                     /gene="map"
                     /locus_tag="VLS86_03390"
     CDS             1402..>1955
                     /gene="map"
                     /locus_tag="VLS86_03390"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HSO49566.1"
                     /translation="MITIKSRREFVKMQRAGAAVAAIHEAVREAAGPGVALLDLEAIS
                     ARILEERGCKSSFLGYHGSYPATLCLSPNDVVVHGIPSDRRLEEGDILSVDAGAIYEG
                     FHGDAAFTMAIGRVSDDARRLIEVTEDALWAGIREVRKGNHLGDIGATVQEVGEGAGF
                     GVVEEYVGHGIGRQMHEEPQVPNYG"
BASE COUNT          324 a          629 c          641 g          361 t
ORIGIN      
        1 ggggtgggca acgggatgtc gctgatcatc ttcgtgtcca tcctcagcca gttccccttc
       61 atcttcggtc agatctgggg ccaaacggtg caacaagacc tcccgatacg gttcgcggtc
      121 atcgtggcca tcttcctggc gatgatcgtt gccatcatct acgtcgacca ggcgcaacgg
      181 cggatcccga tccagtttgc gaagcgggtc cgtggccggc gggtgatggg tggccagtcg
      241 acctacatcc cgctctaggt caacaccgcc ggtgtgatcc cggtgatctt cgccacctcg
      301 gtgctgttga tcccgtcact gatcatcacc gccattcctt ccgatgccgc atgggtccaa
      361 tcgatctatc gctgggtcga cagcaacctg ggtggtagcg gccaggtcag ctggtggtac
      421 atcctgatcc tgttctcgct gatcatcttc ttcacctact tctacaccgc gatccagttc
      481 gatcccgaga agcaagccga gatgatccag cgtcagggtg ggttcgtccc cggggtgagg
      541 cccggccggc agaccgcccg ccatctcggg ttcatcttga accggatcac cctccccgga
      601 tccatcttcc tcgcagtcat cgccatcctg cccgccatcg ccggggcggt ctggaacatc
      661 cagatcgcct tctccggggt ctcgatcctg atcgtggtgg gtgtcgccct ggagacgatg
      721 aagcagatcg agtcccagct gacgatgagg aactacgaag ggatcctcac ttgagccgct
      781 gtctcctcgc gttgacgctc cctccgtccg tttggaaccg ggacagatga agctgctgtt
      841 cattggtccc cccggcgccg ggaaggggac ccaggccagc cgggtggctg agaggctcgg
      901 catcccccac atctccacgg gtgagatgtt ccgtcatcac gtgtcgagcg gcaccgacct
      961 cggccggcgg gtggacgcca tcatgaagtc aggcgagtac gtccccgacg agatcaccgt
     1021 gtcgatgctc agagagcggc tcgcagagcc ggacgcccgg gccgggttca tcctcgacgg
     1081 cttcccgcgc acggccgggc aggtcgaggc cctggatggg ttgatcgggg cggacgggct
     1141 cgaccatgtt gtggtgttgg aggccgacga ggacgagctc gtggagcgac tgctggccag
     1201 gggccgcgcc gacgacaccg atgccaccgt gcgcaaccgg ttcgccgtct accaggatca
     1261 gactgcgccc ttgctcgacg tctaccgggg ccggggatcc ctggtccatg tgagtgggat
     1321 gggcccgatg gacgaagtca ccgagcgcat cctctccgcc ctggcgccac cagaaccagg
     1381 gcgagtcgaa gagggcaccc catgatcacc atcaagtcac gacgtgagtt cgtcaagatg
     1441 cagagggcgg gtgctgccgt ggcagccatc cacgaagcgg tccgtgaggc cgccggtccc
     1501 ggggtcgccc tcctcgatct ggaggcgatc tcggcccgaa tcctcgagga gaggggttgc
     1561 aagtcgtcct tcctcggata tcacggcagc tacccggcga ctctgtgtct gtcgcccaac
     1621 gacgtcgtgg tccacggcat cccgtcggat cgtcggctgg aagagggcga catcctctcg
     1681 gtggacgccg gcgccatcta tgagggcttc catggcgatg cggccttcac catggcgatc
     1741 gggagggtct ccgacgatgc ccggaggctg atcgaggtga ccgaggacgc cttgtgggcc
     1801 gggatccgcg aggtccgcaa ggggaatcac ctgggagaca tcggcgccac cgtccaggag
     1861 gtcggcgagg gcgccggctt cggggtcgtc gaggagtacg tggggcacgg tatcggccgt
     1921 cagatgcacg aggagcccca ggtgcccaac tacgg
//
LOCUS       DATITW010000242         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_772926, whole genome shotgun sequence.
ACCESSION   DATITW010000242 DATITW010000000
VERSION     DATITW010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772926"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VLS86_03395"
     CDS             <1..472
                     /locus_tag="VLS86_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49567.1"
                     /translation="VQAGTRSLLDRSHREDLAAAAAGTRPGLIGASETARIVTEFGES
                     FTRRRARVSVAVSEQAQRAKDVLKDALGEERTNALKRLLGRHPTPITNRAEVRLVDEV
                     VEPSPGPTPLLVTGDLDGTILRGPIPVEHILPGSSDGYRQRRLEMIHDYYDVIG"
     gene            complement(509..1609)
                     /locus_tag="VLS86_03400"
     CDS             complement(509..1609)
                     /locus_tag="VLS86_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HSO49568.1"
                     /translation="MVNPLKRLILVLIALAAFIVVAGPASATGFDPNSMTFPIDGEHR
                     ITDSFGDCRGTNCSRSHEGVDMPAAKGIPVVAAGDGEVTWISSGPSTCCFLGIDHGDG
                     WLTRYIHLNDDAQDADGHYLLNTDGQGWGIAEGIVKGTQVTAGQVIGWVGDSGNASEG
                     HPHLHFELRRNGTAIDPYQYLLRAKEVWTGTFRDDDASVHQENIDKIFAQGITVGCNP
                     PTNDRFCPDRNITRGEMAAFIARALNLTAVTGTTPYDDVESHLFAVAVDKLQTAGIGF
                     GCDADSFCPDRPLERDEMAEMLVRAFTYENPDATDFFTDDSGNEFEESINKLAASGIT
                     VGCNPPANDNFCPDRQLTRAEMATFFVRSLGL"
     gene            1818..>2164
                     /locus_tag="VLS86_03405"
     CDS             1818..>2164
                     /locus_tag="VLS86_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49569.1"
                     /translation="MNSLNSRQRFLVLFLAIGAGLLAWLWEELDPLLRGVTVLVVVLV
                     AATAVIRREQRPEASTADPRLDLVERVLADETRRPWSPSTLPWLPTGSSPKPLLLVPA
                     ADYHLAEIVPIAR"
BASE COUNT          390 a          702 c          678 g          394 t
ORIGIN      
        1 agttcaggca gggacgagga gtctcctcga ccggtctcac cgggaagatc tcgcagccgc
       61 tgccgccggc actcgccccg gtttgatcgg ggccagcgag acagcccgaa tcgtcaccga
      121 gtttggtgag agcttcaccc gtcgccgggc ccgggtctct gtcgccgtct ccgagcaagc
      181 gcaacgggcc aaagacgtgc tcaaagacgc cttgggcgag gagcgaacca acgcactcaa
      241 gcgcctcctc ggccgccacc ccacgccgat cacaaacagg gcggaggtcc gtctggttga
      301 tgaggtggtc gagccctccc ctggcccgac gccgctgttg gtcaccgggg atctggacgg
      361 gaccatcttg cggggcccga ttcccgtcga gcacatcctg cccggatcca gcgatgggta
      421 tcgacagcgc cggctggaga tgatccacga ctactacgac gtgatcggtt gaagcccgct
      481 cgaagaggga acgaggtgct caccggtgct agagaccgag ggacctcacg aagaacgtcg
      541 ccatctcggc tctggtgagc tgacgatcgg ggcagaagtt gtcgttggcc ggcgggttgc
      601 accccacggt gatccccgaa gcggccagct tgttgatcga ctcctcgaat tcgttcccac
      661 tgtcgtcggt gaagaaatcc gtggcatcgg ggttctcgta ggtgaaggca cggaccagca
      721 tctcggccat ctcatcccgc tccagggggc gatccgggca gaagctgtcg gcatcacacc
      781 caaagccgat gccggcggtt tgcagcttgt ccaccgccac tgcgaagaga tgactctcga
      841 cgtcgtcata tggggtggtt ccggtgacgg cggtcagatt cagggcccgg gcgatgaagg
      901 ccgccatctc ccccctggtg atgttgcggt cggggcagaa cctgtcattg gtcggcgggt
      961 tgcaaccgac ggtgatcccc tgggcgaaga tcttgtcgat gttctcctgg tgcacggaag
     1021 cgtcgtcgtc cctgaaggtc cccgtccaca cctccttggc gcggaggagg tactgatagg
     1081 ggtcgatcgc cgtgccattc ctgcgcagct cgaaatggag atggggatgt ccctcgctcg
     1141 cgttgcccga atcccctacc cagccgatga cctggccggc cgtcacctga gtgcccttca
     1201 cgatgccctc ggcgatcccc cagccctgcc cgtcggtatt gagcagatag tggccgtcgg
     1261 catcctgagc atcgtcgttg agatggatgt atcgggtgag ccagccgtcc ccatgatcga
     1321 tgccgagaaa acagcaggtc gaagggcctg agctgatcca ggtcacttcg ccgtctccgg
     1381 cggcgaccac cgggattccc ttggcagccg gcatgtcgac gccttcatga cttcgcgagc
     1441 agttggtccc acgacaatca ccaaagctgt ctgtgatccg atgctcacca tcgatgggga
     1501 atgtcatcga gttggggtcg aaaccggtgg cagaggcagg accggcaacc acgatgaacg
     1561 cggccagagc tatgaggacg agaatcagtc gtttcagggg gttcaccacg tcttactccc
     1621 cctatcggac ggatatcgac gactcttgag tgtcatccca ggaccctcat cgggactagc
     1681 ccgagcgagc cgagcgcgtc cgtggcggtt gctccagagc acagcaagca aggcagccaa
     1741 gtcaatgacg gtccagaacg caaaccgttt ggtacctccg gccgcgtcgc ggccgcgata
     1801 ccgtcacccc tagaaacatg aattcgctca actctcgaca gagattcctc gtgctcttcc
     1861 tggccattgg cgccggtctc ctggcctggc tctgggagga actggacccg ttgctgcgtg
     1921 gagtcacggt gctggtggta gtgctggtcg ccgccacagc cgtgatccga cgcgagcaac
     1981 ggccggaggc cagcaccgcg gacccacggc tcgatctcgt cgaaagggtg ctcgctgacg
     2041 agacccgacg gccgtggagc ccctccactc tcccctggct ccccaccggg tcttcgccga
     2101 agccgttgct cttggtccca gccgccgact atcacctcgc cgagatcgtg ccgatcgccc
     2161 gtga
//
LOCUS       DATITW010000243         5608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_572815, whole genome shotgun sequence.
ACCESSION   DATITW010000243 DATITW010000000
VERSION     DATITW010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572815"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1019
                     /locus_tag="VLS86_03410"
     CDS             87..1019
                     /locus_tag="VLS86_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSO49570.1"
                     /translation="MNEATRSPDAFTITAFAVAVTLGGANFLAVRFSNRELEPFWGAG
                     LRFSLAAVLFVVIAVVLRLRWPRGRQLWLTCVYGVFSFALSYALMYWALVRVTAGMAT
                     VVLAVVPLVTLLLAAAQRLEPLGRRTVLGSLLALVGILFSTLGEEVMLPVSGLLAMLA
                     AALTLGQSVILGKRLSGNHPVMTNAVGLATGAPLLLGLSTLAGETWVLPRQPEVVWSV
                     AYLVTLGSVGLFVLIRLVVRRWTASATSYVFVLFPVVTMLLGALLADEPLTARGVIGA
                     VVVMSGVWFGALAPSARQVVAPPREPPTAAPKMA"
     gene            complement(1081..1965)
                     /locus_tag="VLS86_03415"
     CDS             complement(1081..1965)
                     /locus_tag="VLS86_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO49571.1"
                     /translation="MSGRALEVDGDLRWVRVEGTGRPVVVFEPGIGDVGLTWGLVQPA
                     VSETTTTFTHDRPGLGGSEPASGSWGVGAMVDDLRSALSAAALEPPYLLVGHSFASLT
                     VRAFAHRFPEEVSGLLLVDGAHEDQVSRFPPELNPTPMLAGFAVQLRDLAARARSGET
                     IPEMMPVPESFPGDLAGAYRAATAPTPIRLETVAGEYEALPQSQQEVSGLAALGHGDL
                     PLLVMRHGVPQPVPGVSEEVNRRYEEAWQQLQTELAARSTAGEVQVAEGAGHMIHHQR
                     PDLVIAASERMIQGRGKE"
     gene            2075..3289
                     /locus_tag="VLS86_03420"
     CDS             2075..3289
                     /locus_tag="VLS86_03420"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSO49572.1"
                     /translation="MTRILTGQQLAEESGVAVDLIDVLTEMRVVTPNRDGLYSAGDVI
                     RVESVGAFLDAGMSLEILTEGIDRGLFTFEYLDRFYPEPAPLTDRSATELARELGVPA
                     DLLGSVYLAMGLPEPASQRPLRDDEVEIVRSFLEVWSPADPDAVLRAARLMGESARMA
                     SEGWTRLYVEKIADPMAAKGMPMDQRIQTIVETTERATHLAPAMLTWLLQRHMRHSVD
                     QSNIEGLEASMAEHGLVVPGPPDLPAIAFVDVSGYTFMTETFGDDIAVRTSELVRERG
                     QEVVRRHGGSVVKILGDGAMLHFGRVGEAVTAALALVEALGAEGLSAHAGVHSGTVIQ
                     HDGDYYGRTVNLASRVAGEAGPGQVVVTKQVVDGAESDRFVFESLPPVSLKGIDQPVE
                     LYRAWLASGRRS"
     gene            complement(3321..4100)
                     /locus_tag="VLS86_03425"
     CDS             complement(3321..4100)
                     /locus_tag="VLS86_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49573.1"
                     /translation="MTATPELGVHSGPVYWLRGYRSMLRFETKNLRAYLTIGLVIQVF
                     MGAGMAVMYGFYFGDLSLEQRTFLVTGIPALALVPIGFVMVPSAIMEHKLRDTYDYVW
                     SLPVPRLSSAMATFTIFTALAIPGTAIALIIASVVHGVDLQVSWAVVPAILLTSALAT
                     SVGYALGHSVPEPRVTNLITNLVIFLVLLFSPIVVPIEQFPDWWEAVHRVLPFWHMSV
                     VIRAGLTEGMITSSVAASYVVLLIWCLVSWLITAWVIGRRG"
     gene            complement(4097..4870)
                     /locus_tag="VLS86_03430"
     CDS             complement(4097..4870)
                     /locus_tag="VLS86_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49574.1"
                     /translation="MTTGLPAKRVGVRNWLHSYRTMLRWEVVGSRLFLPLFVIVQILA
                     GAGFVIGFGLLIPQVDANTALYLSTGAVVMSLILIGLLVTPQLVAQQKMEGSYEYLWS
                     LPVPRSASTLASTTLSALVAIPGVLAALLVAVWRYDVSFTIHPTVVPAFVLTLLCGSL
                     IGSAVAHGVGNPQMTMLFTQLGIFFIIGFSPVSFPIERLPTWLATVHEYLPIHHMALA
                     VRSSLTDGLVEMTGRSWVVLVVWTVAAAVVTGLVLVRRK"
     gene            complement(4882..>5608)
                     /locus_tag="VLS86_03435"
     CDS             complement(4882..>5608)
                     /locus_tag="VLS86_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO49575.1"
                     /translation="EDPAAARQLCSYLPQAQMPIDSFKVREAIEIAGMIRGGAETDIR
                     RRCDSLIEALQIVEWGDTLGVKLSGGVRRLVGFAMATVHPGRIVFLDEPTNDVDPLRR
                     RLLWDQIRLVADSGSAVFLVTHNVLEAEKSVDRLAVIDGGRLIAEGTPSSLKADDRGR
                     MRLLVMMVPGAADPDLPACVESTTRVGNHLATVISERDVSSGIEWAQGLIADGLAEEY
                     AVGATSLEDAYIRLTGHVSGDGS"
BASE COUNT          992 a         1843 c         1848 g          925 t
ORIGIN      
        1 tcgggaaatg gggtgttcac ccgatgccga ttcccttccg atcaggcagt gtcgtcgctc
       61 tggagccctg aaggaggacc accccaatga acgaggcaac ccgatctccg gacgcattca
      121 ccatcactgc ctttgccgtg gctgtcaccc tcggcggagc caactttctg gctgtccgct
      181 tcagcaaccg cgagctcgag ccgttctggg gggcgggatt gcggttcagc ctggctgctg
      241 tcttgtttgt ggtgatagcc gtcgtgttgc gcctgcgctg gccccgcggg cgacaactct
      301 ggctgacctg tgtctacggg gtgttcagct tcgccctctc ctatgccctc atgtactggg
      361 cgctggtgcg ggtcaccgcc gggatggcaa cggtggtgct ggcggtggtc cccctggtga
      421 cgcttctcct ggcggcggcg cagcgactgg agcctctggg ccggcggaca gtgctgggct
      481 cgcttctcgc cctggtcgga atcctgtttt cgacgctagg tgaggaggtg atgctcccgg
      541 tgagcggtct gctggccatg ctcgccgccg ccctgaccct gggccagagc gtcatcctcg
      601 gcaaacggct gtcgggcaac cacccggtga tgaccaacgc ggtgggcctc gccaccggcg
      661 caccactcct gctcggtctc tcgacgctgg ccggggagac ttgggtcctg ccccgacagc
      721 ccgaggtggt gtggtcggtg gcctacctgg tcacgctggg gtcggtcggg ctgttcgtgc
      781 tgatccggtt ggtggtgcgc cggtggaccg cctccgcgac ttcgtacgtg ttcgtcctct
      841 tcccggtggt caccatgctc ctcggtgcct tgctggccga cgagccgctg acggctcgag
      901 gggtcatcgg cgccgtggtg gtcatgtccg gggtgtggtt cggggccctc gccccgtcgg
      961 cccggcaagt cgtcgcgcca ccgcgagaac ctccgacagc cgctcccaag atggcgtagc
     1021 tcaggtcagc accatcgacc agtacctcag ggcggcgtga tcacggctgt cgactgacga
     1081 tcactccttc ccgcgaccct ggatcatccg ttccgacgcg gcgattacca ggtctggacg
     1141 ctggtgatgg atcatgtgcc ccgccccttc cgcaacctgc acctcgccgg cggtggaccg
     1201 tgcagccagc tcggtttgca gctgctgcca ggcctcctcg tagcgacggt tcacctcttc
     1261 actcacgccc ggaaccggct gtgggacgcc gtgacgcatg accagcaatg ggaggtcgcc
     1321 atggccaaga gccgccagcc cactcacctc ctgctgactc tgagggaggg cctcgtactc
     1381 gccggccacg gtctcgagcc ggatcggcgt gggggcggtg gcggcccggt aggcgccggc
     1441 gagatcgccg ggaaagctct cgggtaccgg catcatctcg gggatcgtct cgccggaccg
     1501 tgctcttgcc gccaagtctc gaagctggac ggcaaacccg gccagcatgg gcgtcgggtt
     1561 tagctcaggc gggaacctcg acacctggtc ttcgtgggca ccgtcgacca gaagaaggcc
     1621 gctcacctcc tcggggaagc gatgggcgaa cgccctgacc gtcagcgagg cgaaggagtg
     1681 accgaccagc aggtacggag gttcgagggc ggctgcggac aacgccgatc tcagatcgtc
     1741 gaccattgcc cccacccccc atgaccctga cgccggctcg ctgccgccga gaccgggacg
     1801 atcgtgggtg aaggtggtcg tagtctcgga aaccgcaggc tggacgaggc cccaggtgag
     1861 acccacatcg ccgatccccg gctcgaagac cacgaccggg cggccggtcc cctcgaccct
     1921 gacccagcga aggtctccat cgacttcgag ggccctgcca ctcatctccg ccctttcctt
     1981 ccccaagcgt gcgccgaggc tatcggaccg ctctcctgcc acgaggtgac aagaaccagg
     2041 ccgaggacac cgatccacca cttaggatcg gatcgtgacc cgcatcctga ccggtcagca
     2101 gctcgccgag gagtcaggag tggctgtcga cctgatcgac gtgctgaccg agatgagggt
     2161 ggtcactccc aaccgtgatg gcctgtactc ggcgggggac gtcatccggg tggaatcggt
     2221 tggggccttt ctcgacgccg ggatgtcact cgagatcctg actgagggaa tcgacagagg
     2281 tctgttcacc ttcgagtatc tcgaccgctt ctacccggag ccggcccccc tgaccgatcg
     2341 gagcgccact gagctggctc gggagttggg tgtccctgcg gacttgctgg gatcggtgta
     2401 cctggcgatg ggtctgcccg aaccggcttc ccaacgacca ctgcgcgatg acgaagtgga
     2461 gatcgtccgg tccttcctcg aagtctggtc gcctgccgac cctgatgcgg tgctgcgagc
     2521 cgcccggctc atgggtgagt cggcgcgaat ggcgtccgag ggatggaccc ggctctacgt
     2581 cgagaagatc gccgacccga tggcggccaa agggatgcca atggaccagc gaattcagac
     2641 catcgtcgaa accaccgaga gagcgaccca cttggcgcca gcgatgctca cctggctcct
     2701 gcaacgacac atgcgccatt cggtcgacca gtcgaacatc gaggggctgg aggcttccat
     2761 ggccgaacac ggcttggtcg tgcccggacc gcccgatctc cccgcaatcg ccttcgtcga
     2821 cgtgtccggc tacaccttca tgaccgagac gtttggcgac gacatcgcgg tccggacctc
     2881 cgagctggtg agagagcgcg gtcaggaggt ggtccgcaga catgggggct cggtggtcaa
     2941 gattctcggg gacggcgcca tgctccattt cggccgggtg ggcgaggcag tgactgccgc
     3001 gttggcgctg gtcgaagccc tcggcgcgga gggcctgtcg gcccacgccg gagtgcactc
     3061 cggcactgtc atccaacacg acggggacta ttacgggcgc acggtgaatc tggcctcccg
     3121 ggtggccggg gaagcggggc ccggtcaggt ggtggtcacc aagcaagtag tggatggcgc
     3181 cgagtcggac cgattcgtgt tcgagtcgct ccccccggtc agcctgaagg ggatcgatca
     3241 gccggtagag ctctaccggg cctggttggc aagcgggcga aggagttgac cgccgtctct
     3301 gaggcgcccg cgcctgctcc tcagccgcga cgcccaatca cccaagcggt gatgagccac
     3361 gacacgagac accagatcaa gagaaccaca taggacgcgg cgaccgatga ggtgatcatc
     3421 ccttcggtga gcccggcccg gatcaccacc gacatgtgcc agaagggcag gacccggtgc
     3481 acggcctccc accagtcggg gaactgctcg attggcacca cgatcggact gaacagcagg
     3541 acgaggaaga tcaccaggtt ggtgatgagg ttggtcactc tcggctccgg aaccgaatgc
     3601 cccaatgcgt agccgaccga ggtggccagc gccgaggtga gcaggatggc gggcaccacc
     3661 gcccacgaga cctgcagatc gaccccatgc accacggagg cgatgatgag agcgatggca
     3721 gtgccaggga tggcaagagc ggtgaagatg gtgaaggtcg ccatggccga cgacaacctc
     3781 ggcaccggga gtgaccacac atagtcgtat gtgtcgcgca gcttgtgctc catgatggcg
     3841 ctggggacca tgacgaaacc gatcggcacc agggccagcg ccgggatccc ggtgacgagg
     3901 aacgtcctct gctccaggct caggtcaccg aagtagaagc cgtacatcac agccattccc
     3961 gctcccatga aaacctggat gaccaacccg atggtcagat aggcgcggag gttcttcgtc
     4021 tcgaatcgaa gcatcgacct gtacccgcgc agccagtaca cgggcccgga gtgcacgccg
     4081 agttcagggg tcgcggtcat ttccgacgca ccaacaccag accggtgacg acagcggcgg
     4141 caacggtcca aaccacgagc accacccaac tccgaccggt catctccacc aggccgtcgg
     4201 tgagcgacga ccgcacggcg agtgccatgt ggtgtatcgg caggtactca tggacggtgg
     4261 cgagccatgt ggggagacgc tcgatcggaa agctcacagg ggagaaaccg atgatgaaga
     4321 agatgccgag ctgggtgaag agcatcgtca tttgaggatt gccgaccccg tgcgcgaccg
     4381 ccgagccgat caggctgccg cagagcaagg tgaggacaaa ggcggggacg acggtgggat
     4441 ggatcgtgaa actgacgtcg taccgccaca cggcgacgag cagtgccgcc agcaccccag
     4501 gtatcgccac cagagcggaa agggtcgtcg atgccagggt ggacgcgctc cgtggcaccg
     4561 gcagcgacca gaggtactcg tacgagccct ccatcttctg ttgcgccacg agctgtggtg
     4621 tgaccaggag gccgatcaag atcagcgaca tcaccaccgc cccggtcgag aggtagaggg
     4681 ccgtgttggc gtcgacctgg gggatgagca gcccgaaccc gatcacgaag ccggcgccgg
     4741 ccaggatctg gacgatcacg aacaagggga gaaacaaccg cgagccgacc acctcccagc
     4801 ggagcatggt ccggtagctg tggagccaat tgcgcacgcc gacccgcttc gccggcaggc
     4861 ccgtcgtcat cacatctgcg gtcaagagcc gtccccgctc acatggccgg tcagccggat
     4921 gtaggcgtcc tcgagcgagg tcgcccccac ggcgtactcc tcggccaacc catcggcgat
     4981 cagcccttgc gcccattcga ttcccgagga gacgtcccgc tcgctgatga cggtggccag
     5041 atgattgccg acccgtgtcg tgctctcgac acacgccggc aggtccgggt cggccgcacc
     5101 cgggaccatc atgaccagca ggcgcattcg tccccgatca tcggccttca gggaggaagg
     5161 cgttccctcg gcgatgaggc ggcctccgtc gatcaccgcc agccggtcga ccgacttctc
     5221 ggcttcgagc acgttgtggg tgaccagaaa cactgccgat cccgagtcgg ccaccaagcg
     5281 gatctgatcc cagagcagac gtcttcgcaa cgggtcgacg tcgttggtcg gctcatcgag
     5341 aaagacgatc cgaccggggt gaaccgtcgc catggcaaac ccgacgagac ggcggacccc
     5401 gcccgacaac ttgaccccga gcgtgtcacc ccactcgacg atctgaagag cctcgatcag
     5461 cgagtcacag cgacgccgga tgtcggtctc cgcgccgccc ctgatcatcc ctgcgatctc
     5521 gatcgcctct ctgaccttga acgagtcgat cggcatctgg gcctggggga gataggagca
     5581 gagctggcgg gccgccgccg ggtcttcg
//
LOCUS       DATITW010000244         1622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_412497, whole genome shotgun sequence.
ACCESSION   DATITW010000244 DATITW010000000
VERSION     DATITW010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412497"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /gene="mraY"
                     /locus_tag="VLS86_03440"
     CDS             <1..667
                     /gene="mraY"
                     /locus_tag="VLS86_03440"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HSO49576.1"
                     /translation="LAIAALFAWGASMAGVVTEISFVRPLGIDLGAFFVVWVLLMLTG
                     FANGVNLADGMDGLAGGSSALVVASYTVIGFWQFRNADVYDAVDPLELAIVSAAMFGA
                     ILGFLWWNAPPAKINMGDVGSQGIGGLLAALALLTNTHLLLLVLGGIFVAETVSVILQ
                     VASFRLFGKRIFRMSPIHYHFDLGGWPETTVVIRFWILTGIGVALGLGFFYGDFLLLE
                     GLL"
     gene            664..>1622
                     /gene="murD"
                     /locus_tag="VLS86_03445"
     CDS             664..>1622
                     /gene="murD"
                     /locus_tag="VLS86_03445"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HSO49577.1"
                     /translation="MNRVLVLGAGVSGAAAARLARSRDLAVTVYSEETSAEMLGQGFS
                     VASGEWDPVLLNGVDLVITSPGFSERALPIVEALEWGVPVWSEIEYAWRQIEIPVAAV
                     TGTNGKTTVTEATSAMLTESGIDAPATGNIGSALSDHVDEDHDAFVIEVSSFQLRFCE
                     SFHPTSAALTNVAADHLDWHGSEYAYRSAKARIFANQTENDLLVYDADDEGATSLVSS
                     APSHLFPVSGLHLPAGGGGIDEGRLVVGEMSMAIDDLTSDDPTHLVNMATASALALRM
                     GASPEGVMRAARSYRPGAHRRSIVGRAEGVTWIDDSKATNPHAA"
BASE COUNT          235 a          537 c          530 g          320 t
ORIGIN      
        1 gctggcgatc gcagctctct tcgcctgggg cgcctcgatg gcaggggtgg tcaccgagat
       61 ctcattcgtc cgacccctcg gcatcgatct cggagcgttc ttcgtggtct gggtgctgct
      121 catgctcacc ggtttcgcca acggggtgaa cctcgccgac gggatggacg ggctggccgg
      181 cgggtcctcc gcgctggtag ttgcctcgta caccgtcatc ggcttttggc agttcaggaa
      241 cgccgacgtg tacgacgcgg tcgatccgct cgagctcgcc atcgtctctg cggccatgtt
      301 cggcgccatc ctcggctttc tgtggtggaa cgcccctccg gccaagatca acatgggtga
      361 tgtcggctcg caggggatcg gtgggcttct ggccgcgctc gcgctgctca ccaacaccca
      421 tcttctcctc ctggtcctgg gtgggatctt cgtcgccgag accgtgtcgg tgatcctcca
      481 ggtggccagt ttcaggctgt ttgggaagcg gatcttccgc atgtccccga tccactacca
      541 cttcgatctc ggtggctggc cggagaccac cgtcgtcatc aggttctgga tcctcaccgg
      601 aatcggggtc gccctcggtc tcgggttctt ctacggcgac ttcctgctcc tcgagggtct
      661 gctgtgaacc gggtgctcgt gctcggggcc ggggtgtcgg gggcggctgc cgcccgcctg
      721 gcgaggagtc gtgacctcgc cgtcaccgtc tactccgaag agacctcggc ggagatgctg
      781 gggcaggggt tctctgtcgc cagcggcgaa tgggacccgg tattgctcaa tggcgtcgac
      841 ctcgtgatca ccagcccggg gttctccgag cgagcactgc cgatcgtgga ggcgctggag
      901 tggggggtgc cggtttggag cgagatcgag tatgcctgga ggcagatcga gatcccggtg
      961 gccgcggtga ccgggaccaa tggcaagacc acggtcaccg aagcgacttc cgccatgctc
     1021 acagaatcgg ggatcgacgc cccggcgacg gggaacatcg gctccgctct ctccgatcac
     1081 gtcgatgagg accacgacgc attcgtaatc gaggtgtcga gcttccagct ccgattctgc
     1141 gagtcgttcc acccgacgtc ggctgctttg accaatgtcg ccgcggacca tctcgactgg
     1201 cacggctccg agtacgccta tcgatcggcc aaggcccgga tattcgccaa tcagaccgag
     1261 aacgacctgt tggtctacga cgccgacgac gaaggtgcca cctccctcgt ctcctccgcc
     1321 cccagccatc tcttcccggt ttcggggctt catctccctg ccggtggcgg gggcatcgac
     1381 gagggccgtc tggtggtcgg tgagatgtcg atggcgatcg acgacctgac ctccgacgat
     1441 ccaacccacc tggtgaacat ggctaccgcg tcggcactgg ctctgcggat gggtgcgagc
     1501 cccgaggggg tgatgagggc ggcccgctcc tatcgccccg gcgcccaccg ccggtcaatc
     1561 gtcggccggg ccgagggggt cacctggatc gacgactcca aggccaccaa cccccatgcc
     1621 gc
//
LOCUS       DATITW010000245         1606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_733099, whole genome shotgun sequence.
ACCESSION   DATITW010000245 DATITW010000000
VERSION     DATITW010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733099"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VLS86_03450"
     CDS             complement(<1..331)
                     /locus_tag="VLS86_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSO49578.1"
                     /translation="MSFDVCIIGAGLVGLGVGRALAETHSGIELVVVDKESTVATHQS
                     GHNSGVAHSGLYYRPGSLKARLCVEGRRELKEFCGAEGIAFDESGKLVVATSASEIPA
                     LDELELRG"
     gene            474..1163
                     /locus_tag="VLS86_03455"
     CDS             474..1163
                     /locus_tag="VLS86_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSO49579.1"
                     /translation="MAGAVLIVGGTGGIGRELALHYLEKGREVIITGRDVAKVQAVAS
                     ELGGGAKGLGFDLGRPETIAAALAEVGAVDKLVLSAIDRDNNPVREYNIDGARNLATM
                     KIVGYTETVHTLIPKLSDESAVVLFGGRAKDRPYPGSTTVTAVNGAVSTMITTLAIEL
                     APIRFNAIHPGIVGDSPFWEGKQLDGILARTPTGRLATMEDVVDATVFLLENRSVNGI
                     NLYVDGGWLLM"
     gene            1213..>1606
                     /locus_tag="VLS86_03460"
     CDS             1213..>1606
                     /locus_tag="VLS86_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSO49580.1"
                     /translation="MTEDLEGIGDRLRDERTRAGISQRELARRLGLSASLISQLESGL
                     SKPSVGTLYAIVTELDLSLDKLLRGDEHAGRDHAGSDGAADNGRRSPLIHPEQRKAID
                     LASGVRWEQLTSDTEEGVDFLHAIYEVGG"
BASE COUNT          291 a          533 c          521 g          261 t
ORIGIN      
        1 ttccccgcag ctccaattcg tccagggccg ggatctccga ggcagaagtc gccaccacga
       61 gtttgcccga ctcgtcgaag gcgatcccct cggccccgca gaactccttg agctcgcgcc
      121 ggccctccac gcagagacgg gctttgagcg agccgggtcg gtaatagaga ccggaatgcg
      181 cgaccccgct gttgtggccg ctctgatgag tcgccaccgt cgactccttg tcgacaacga
      241 ccaactcgat gccgctgtgg gtttcggcca gcgcacggcc cacccccaga cctacgagac
      301 cagcgccgat gatgcaaacg tcaaagctca cgcgcgagcc tggttcttgt cacgttgtag
      361 atctcctggc gcggggcgac agcatacccg tggccccatg ggtgctcagt ctcactgcac
      421 agtgcacccc tcgccgggct aggatccgac ccgacagcaa ggagcgcatt ggaatggcag
      481 gggcagtgtt gatcgtgggt ggcaccggtg ggatcggccg cgagctcgcg ctccactacc
      541 tggagaaggg gcgggaggtg atcatcaccg gccgcgatgt cgccaaggtc caggctgtcg
      601 cctcggagct cggtggcggg gccaagggcc ttgggttcga cctgggacgg ccggagacga
      661 tcgccgccgc actggccgag gtgggggccg tcgacaagct cgtcctttcc gcaatcgacc
      721 gcgacaacaa cccggtgcgc gaatacaaca tcgatggcgc ccggaacctg gcgacgatga
      781 agatcgtcgg ttacaccgag acggttcaca ccctgattcc gaagttgtcc gatgagagcg
      841 ctgttgttct cttcggggga cgggccaagg accggcccta cccggggtcg acgacggtga
      901 ccgcggtgaa cggggcggtg tcaaccatga tcacgaccct ggccatcgag ctggcgccga
      961 tacgcttcaa cgccatccac cccgggatcg tgggcgacag cccgttctgg gagggaaagc
     1021 agctcgatgg catcctggcc cgcactccga ccggccgtct cgccaccatg gaggatgtgg
     1081 tcgacgccac cgtctttctc ctcgagaacc gatctgtgaa cggaatcaac ctctacgtcg
     1141 acggtggctg gctgctcatg tagcgccggt gccggcgctc tatcctcgcc accacgatga
     1201 aggtgactcg cggtgactga ggacctggag ggaatcggtg accggctccg cgatgaacgg
     1261 accagggcag gcataagcca gcgtgagctg gcgagacgcc tcgggctctc ggcaagtctc
     1321 atctcccagt tggagagtgg cctctccaag ccgtcggtgg gcactctgta cgcgatcgtc
     1381 accgagctcg acctctccct cgacaagctg ctgcgtgggg acgagcacgc gggacgggac
     1441 catgccggtt ccgacggggc ggccgacaac gggcgtcgca gccccctgat ccaccccgag
     1501 cagcgaaaag caatcgacct cgcctctggc gttcgctggg agcaactcac ctcggacacc
     1561 gaggaaggcg tcgacttcct ccacgccatc tacgaagtcg gcgggg
//
LOCUS       DATITW010000246         2479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_252220, whole genome shotgun sequence.
ACCESSION   DATITW010000246 DATITW010000000
VERSION     DATITW010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252220"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1816)
                     /locus_tag="VLS86_03465"
     CDS             complement(<1..1816)
                     /locus_tag="VLS86_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49581.1"
                     /translation="MRGRDQTFLADLIGPEDQAIEAVVSRYQRVAPAVTRFARTLSKN
                     PNLRVRLGSEAAASDDEVVCDPRIFQAAYHRNAPVTPDEVALASALHEVMHLVTTNLD
                     EKRELPQGWPVEGEVPDDAMVDLLTALERTNKPVAEVLFFTLEDARQEHQGLSVYPGA
                     RSVLEDLYLSSLGQAIARSSSLSQFVLGCFLLTGGYVEKDLLEKRFDGRAASAIDDAT
                     HHCDMAAESNDPWEVAQLALELEAIARAHGLITEVQANTTHAKRQTPADSEAEKARDG
                     VDAVRMTSPIVMDAESYQDTRRSSDSRSGASDHKGASDIAGEEGTDQLLRVSQSPDVF
                     LPTGQGGKLVVGPIPASFSRFAGEGSVALAETAKRWGVAQRKVSGELFPLFAANQRRG
                     LRSGFDQGDVSPHAALFIGAGLYQRLYERRAARTRRTYAVSLLVDASASMLSPGKGGG
                     LRGTSWAMPAALLGAWTMAHLCDELQIDFEVALFNRGYAAHPDDTEDSYRRKRAAATA
                     GLRRTQGTAADRLTTTVNHYVVKPFERRWRDTEPALAGLFYTAAEPKKASTLARRDPT
                     TAPPVSLFEKAANVDEFNIIHAAERMARLGAQVRVLMVL"
     gene            1907..>2479
                     /locus_tag="VLS86_03470"
     CDS             1907..>2479
                     /locus_tag="VLS86_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSO49582.1"
                     /translation="MHYDLTTLPNGLRVITEPMPSLRSVALGCWVDTGTRDELPNEQG
                     ASHFLEHLLFKGSEKLSARQVSEIFDAIGAESNAFTSKESTCYWARLLDQDLATGFDV
                     LSEMIQRPAFRQNEIDAERQVVVEEINMNEDDPDDVAFENFSTAVFIDHPLEAPVLGT
                     RESIRGMSRDDIHGYWERRYGARSMVVAAAG"
BASE COUNT          395 a          800 c          854 g          430 t
ORIGIN      
        1 ccaggaccat gaggactctc acctgtgccc cgagccgggc catgcgctcc gcggcgtgga
       61 tgatgttgaa ctcgtccacg ttggcggcct tctcgaacag agagaccggt ggggcggtgg
      121 tgggatcgcg tcgggccagg gtcgaggcct tcttcggctc cgccgcggtg tagaacagac
      181 cagccagcgc cggttcggtg tcgcgccagc gtcgctcgaa gggcttgacc acatagtggt
      241 tgaccgtggt ggtcagccgg tccgcggccg tgccctgagt gcgtctgagc cccgcggtgg
      301 ccgccgctcg tttcctccga tacgagtcct cggtgtcgtc ggggtgcgcg gcgtagcccc
      361 ggttgaacag ggcgacttcg aaatcgatct gcagctcgtc gcagagatgg gccatggtcc
      421 acgccccgag caaggcggcg ggcatcgccc aagaggttcc ccggagcccg cccccctttc
      481 ccgggctgag catcgaggcc gaggcgtcga cgagaagcga aaccgcgtag gtcctccggg
      541 tccttgccgc tcgccgctcg tagagacgct gatagagccc ggccccgatg aatagtgcgg
      601 catgtggcga cacgtcgccc tggtcgaaac cggagcgcag gccacgccgc tggttggcgg
      661 cgaacagggg gaagagctca ccggagacct tccgctgggc aaccccccat ctcttggcgg
      721 tctcggccag ggcgacgctg ccttcgcccg cgaagcggga gaaagaggcg gggataggcc
      781 cgaccaccag tttgccgccc tgcccggtgg gcaggaagac atcgggcgac tggctgacac
      841 ggagcagttg gtcggtgccc tcctccccgg cgatgtccga ggcgcccttg tggtcggagg
      901 cacccgatct ggagtcgctg gagcgtctcg tgtcctgata cgactcggcg tccatcacga
      961 tcggcgaggt cattcggacc gcatccaccc cgtctcgggc tttctcggct tcgctgtcgg
     1021 cgggggtctg acgtttggcg tgggtggtgt tggcctgaac ctcggtgatc agcccatggg
     1081 cgcgggcaat agcctcgagc tcgagtgcga gttgtgccac ctcccacggg tcgttcgact
     1141 cggcggccat gtcgcagtga tgcgtggcgt cgtcgatggc ggatgcggcc ctgccatcga
     1201 acctcttctc gagaaggtct ttttccacgt acccaccggt gaggaggaaa cagccgagca
     1261 cgaactggct gagcgagctg gatcgggcaa tcgcctgacc cagtgaggag agatagaggt
     1321 cctcgagcac cgatcgggcc cccgggtaga ccgagagccc ctggtgctcc tgccgcgcgt
     1381 cctcgagggt gaagaacagg acctcggcaa ccggcttgtt ggtccgctcc agggcggtga
     1441 gcagatcgac catcgcgtcg tcaggcacct cgccctcgac gggccatccc tggggcagct
     1501 cgcgcttctc gtcgagattg gtcgtcacca ggtgcatcac ctcgtggagg gccgaggcca
     1561 gagccacctc gtcgggggtg accggtgcgt tgcggtggta ggcggcctgg aagatccgcg
     1621 ggtcgcagac cacctcgtcg tctgacgccg cagcctcgga cccgagccgg acccggagat
     1681 tcgggttctt ggacagggtg cgggcgaagc gggtcaccgc cggcgccacc cgctggtatc
     1741 ggctgaccac ggcctcgatc gcctgatctt ccggcccgat caggtcggcg agaaaagtct
     1801 ggtcgcgtcc acgcatagcg ggccattgtc tcatggctgt caaaaagcgg gcacgccggg
     1861 ggagccggcc ggcgtcgtag ccacgtcgcg tccttacact cccgacgtgc actacgacct
     1921 gaccactctt cccaatgggc tccgcgtcat caccgagccc atgccctccc ttcgctcggt
     1981 ggccctcggt tgctgggtcg acaccgggac gcgcgacgag ttgcccaacg aacaaggcgc
     2041 gtcgcatttc ctggagcacc tcttgttcaa gggctcggag aagctgtcgg cgaggcaggt
     2101 gtcggagatc ttcgatgcga tcggcgccga gtcgaatgcg ttcacctcga aggagagcac
     2161 ttgttattgg gcgcgactcc tcgatcagga cctggccacc gggttcgacg tcctttccga
     2221 gatgatccag cgcccggcat tccggcagaa cgagatcgat gctgagcggc aagtggtcgt
     2281 cgaagagatc aacatgaacg aggacgaccc cgacgacgtg gccttcgaga acttcagcac
     2341 ggcggtcttc atcgatcacc cactggaggc cccggtgctg ggcacccgcg agtcgatacg
     2401 cggcatgtcc cgcgacgaca tccatggcta ttgggagcgg aggtatgggg ctcgctccat
     2461 ggtggtggcc gcggccggg
//
LOCUS       DATITW010000247         1632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_292529, whole genome shotgun sequence.
ACCESSION   DATITW010000247 DATITW010000000
VERSION     DATITW010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292529"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VLS86_03475"
     CDS             <1..318
                     /locus_tag="VLS86_03475"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HSO49583.1"
                     /translation="LGVAVGEPRFVVRIEPATSTVVVGRREELGIAGAELSQLVWTNG
                     DIGKGEIMAQYRAHGEPVPAVLDGDRLRFTHLQEALSPGQTVAFYQGDRVLGGALISG
                     TFV"
     gene            344..>1632
                     /gene="ligA"
                     /locus_tag="VLS86_03480"
     CDS             344..>1632
                     /gene="ligA"
                     /locus_tag="VLS86_03480"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HSO49584.1"
                     /translation="MSDVQSRIEELRSQVNEHLYRYHVLDDPTISDAQFDALIAELKR
                     LEEEHPELVTPDSPTQRVGAPIGDLFQPVRHLRPLFSLDNAASREDLESWELRLERQL
                     GRPPQGYVAELKIDGLAVVLTYRDGMLASGATRGDGVTGEDITANLRTVKSVPLRLLD
                     KAPPTVLEVRGEVYMPEKAFEELNRRQLEAGDRVFANPRNAAAGSVRQKDPAMTASRN
                     LAIWVYQLGLVEGGPDFESHADTIRYLQDLGFRTNPASQELGSIEAVLDYVATAERQR
                     HDTGYQTDGVVIKVNSLGEQEALGFTSRAPRWAIAYKFPAEEQVTTLKDIWINIGRTG
                     AATPFAVLEPVFVGGATVGLATLHNEDQVALKDVRIGDQVIVRRAGDVIPEVVGPVLA
                     ARTGKEKKWKMPSKCPFCGNPIVRIEGEAVARCTGGYT"
BASE COUNT          287 a          521 c          552 g          272 t
ORIGIN      
        1 cttggggtcg ccgtgggcga accgagattc gtggttcgga tcgaacccgc cacctccacg
       61 gtggtcgtcg gcagacgcga ggagctgggg atcgccggag ccgagctgtc tcaactggtc
      121 tggaccaatg gtgatatcgg caagggagag atcatggccc aataccgggc ccacggtgag
      181 ccggtgcctg cggtgctgga cggcgatcgc ctccgtttca cccacctcca ggaagcgctc
      241 tccccgggcc agacagtggc tttctatcag ggcgaccggg tgctgggcgg ggcgttgatc
      301 tccgggacct tcgtgtgaca ggtcgtcggt aacgtccgca tcgatgtcgg acgttcaatc
      361 tcgcatcgag gagctccgct cccaggtcaa cgagcacctc tatcgctatc acgtgttgga
      421 tgatccgacg atctccgatg cccagttcga cgccctgatc gcggagctga agcgcctcga
      481 ggaggagcat ccggagctgg tcacgcccga cagcccgacc cagcgggtcg gggcgccgat
      541 cggggacctg ttccagccgg tccggcatct ccgtcccttg ttctccctcg acaacgcggc
      601 ctccagggag gatctcgaga gttgggagct tcggctggag cgtcaactgg gccggccgcc
      661 gcagggatac gtggccgagc tcaagatcga cgggttggcc gtggtgctca cctaccggga
      721 cggaatgctg gcctccggcg ccacccgggg ggacggggtc accggggaag acatcacggc
      781 caatctgcga accgtcaagt cggttccgct ccggctcctc gacaaggccc cgccaaccgt
      841 cctcgaagtc cgcggcgagg tctacatgcc ggagaaggcc tttgaggagc tcaatcgacg
      901 tcagctcgag gcaggggatc gggtcttcgc caacccgcgc aacgcggcag cgggatcggt
      961 ccggcagaag gacccggcga tgaccgcctc ccgcaacctc gcgatttggg tctatcagct
     1021 cggattggtg gaaggcggcc cggatttcga gtcccatgcc gacaccatcc gctatctcca
     1081 ggacctcggt ttccggacca acccggcgag ccaggagttg gggtctatcg aagcagtgct
     1141 cgactacgtg gccaccgcag agaggcagcg gcacgacacg ggctatcaga ccgatggagt
     1201 ggtgatcaag gtcaactcgc tgggcgagca ggaggcactc gggttcacct cgcgcgctcc
     1261 gaggtgggcg atcgcctaca agttcccggc cgaagagcag gtcacgactc tgaaggacat
     1321 ctggatcaac atcggcagga ccggggcagc cacccccttt gccgtcctcg agcctgtctt
     1381 cgtcggtggc gccaccgttg ggctcgccac cctgcacaac gaggatcagg tggccctcaa
     1441 agatgtgcgg atcggggacc aggtgatcgt ccgccgcgcc ggcgacgtga tcccggaggt
     1501 ggtcggcccg gtgctcgccg cccgcaccgg caaggagaag aaatggaaga tgccttcgaa
     1561 gtgccccttc tgtgggaacc cgatagttcg gatcgaaggc gaggcggttg cccggtgcac
     1621 cggtgggtac ac
//
LOCUS       DATITW010000248         1616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_132111, whole genome shotgun sequence.
ACCESSION   DATITW010000248 DATITW010000000
VERSION     DATITW010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132111"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /gene="heR"
                     /locus_tag="VLS86_03485"
     CDS             complement(<1..573)
                     /gene="heR"
                     /locus_tag="VLS86_03485"
                     /inference="COORDINATES: protein motif:HMM:NF037099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HSO49585.1"
                     /translation="MSSISAPRSRLRGYNIAMGLLHAAQGVIILILATDFTLPVVGTF
                     MTGPPGAAPPEITPLFEVSLAWGVAIFLFVSAAAHLLISSPGIYPWYLRNLDHQRNYA
                     RWIEYSISSSVMVVLIAMLVGISDVAALLALAGVNASMILFGLLQEKYETPGESVSWL
                     AYWFGVLAGAIPWIAIGIYLVSPGTDSAPPG"
     gene            complement(627..>1616)
                     /locus_tag="VLS86_03490"
     CDS             complement(627..>1616)
                     /locus_tag="VLS86_03490"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HSO49586.1"
                     /translation="EKSVVRILAMRRTMFVVPTEMAPILHHSSTVALVAAERKRLVAM
                     VESAGIAADGSAWVDKTSTLTLQALQARGEAVATDLTKDVPELAEKITFYKSDGSVLT
                     TVGMSTRILFLLATEGKVVRGRPKGSWVSSLYRWAPLEDWIGGPMPEIDRARAQSELL
                     IRWLRTFGPGTETDMRWWTGWPVTQVRSALADIGAVEVRVASGPAYVLPDDVEPAEPV
                     RPWVQLLPSLDPTTMGWKERVWYLGPHYPRLFDRNGNAGPTIWVNGQIAGGWAQRGDG
                     EVVWELLEEIGRVAESEVENQAARLRDWLGEKVVIPRFRSPSDRELSGNPPKR"
BASE COUNT          295 a          542 c          499 g          280 t
ORIGIN      
        1 tccgggtggg gcgctgtctg tgccggggct caccagatag atgccgattg cgatccaggg
       61 gatcgccccg gcgagtacgc cgaaccagta cgccagccaa ctcaccgact cgcctggagt
      121 ctcgtacttc tcctggagaa ggccaaagag gatcatcgac gcgttgacac cggcgagggc
      181 gaggagggcc gctacgtccg agatcccgac cagcatcgcg attaggacca ccatcaccga
      241 tgatgagatc gaatactcga tccaacgggc gtaattgcgc tggtggtcca gattcctcag
      301 gtaccacggg tatatgcccg gagatgagat caggagatgg gctgccgcgg agacaaagag
      361 gaagatcgcc acaccccagg ccaggctgac ttcgaacagc ggtgtgatct ctggcggcgc
      421 cgcaccaggc ggtccggtca tgaatgtccc gacaaccggg agggtgaagt cggtggcgag
      481 gatcaggatg atcacgccct gcgccgcatg gagaaggccc atggcgatgt tgtacccacg
      541 aagtcgcgat cggggcgcgg atatggagct catgtgggct attcgtagtt atcagggcca
      601 acggatgaca ggggcgaaat cccttttcac cgttttggtg gattcccgga gagctccctg
      661 tcggacgggg agcggaacct cggaatcacc accttctcgc ccagccaatc ccgcagccgg
      721 gccgcctgat tctccacctc cgattcggcc acgcgcccga tttcctcgag caactcccaa
      781 accacctccc cgtcaccccg ttgcgcccat ccgcccgcga tctgcccatt cacccagatg
      841 gttgggcccg cgttcccgtt gcgatcgaag agacgcgggt agtggggccc gaggtaccag
      901 acgcgttcct tccagcccat ggtggtcggg tcgagcgatg gcagcagttg cacccacggc
      961 cgcaccggct ccgccggctc gacatcgtcg ggaagcacgt aggccgggcc cgaggccacc
     1021 cgtacctcca ctgcgccgat gtctgccaga gcgctgcgga cttgggtcac cggccatccg
     1081 gtccaccatc tcatgtcggt ttcggtcccc ggcccgaagg tcctgagcca tctgatcaga
     1141 agctcggact gggcacgtgc cctgtcgatc tctggcatcg ggccaccgat ccagtcttcg
     1201 aggggcgccc accgatagag actgctcacc cagcttccct tgggcctgcc ccggacgacc
     1261 ttgccttcgg tggcgagcaa gaacaggatc ctggtcgaca tcccgaccgt ggtcagcacc
     1321 gagccgtcgc tcttgtaaaa ggtgatcttc tccgccaact cgggcacgtc cttggtcagg
     1381 tccgtggcca cggcctcgcc tctggcctgc agcgcctgaa gagtcagcgt cgacgtcttg
     1441 tcgacccagg ccgagccgtc agccgcgatc ccggccgact cgaccattgc caccaggcgc
     1501 ttgcgttccg cagccaccag ggccacggtc gacgagtgat gcaggatcgg tgccatctcg
     1561 gtggggacca cgaacatggt gcggcgcatg gcgaggatcc tcaccaccga cttctc
//
LOCUS       DATITW010000249         4075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_12280, whole genome shotgun sequence.
ACCESSION   DATITW010000249 DATITW010000000
VERSION     DATITW010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12280"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /gene="cutA"
                     /locus_tag="VLS86_03495"
     CDS             complement(<1..282)
                     /gene="cutA"
                     /locus_tag="VLS86_03495"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HSO49587.1"
                     /translation="MDDAKRHALTLITCGTTAEAQSIARHLVESRLAAGVQVIPIESV
                     YTWDDEIVEDHEWLLIAKTRRDRFEQIMAAVNEMHPYLVPPVLMIDIDSA"
     gene            complement(334..756)
                     /locus_tag="VLS86_03500"
     CDS             complement(334..756)
                     /locus_tag="VLS86_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HSO49588.1"
                     /translation="MSAKVAVDEIPVFLGTAYAATADQVTKSGLVMTGPPFARMRPLD
                     SEFGQFEIEAGFPVDRAVEIEGEVMSSTLPGGKAAFVTHIGAYDQMMPAYAALETWIV
                     ENQGVPDGPAWEVYYSDPTEQPDPSTWRTEIFQPYKSA"
     gene            941..1474
                     /locus_tag="VLS86_03505"
     CDS             941..1474
                     /locus_tag="VLS86_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HSO49589.1"
                     /translation="MGLASDLGYQLGDQSRMRRWLVELAGTRLVSAVNRRILRPVDRL
                     ALRLTGGNNTLSTWASGIPTLWLTTVGARSGESRTVPLLGIPINDDLAVLGTSFGQKK
                     TPAWVHNLMAHPDAVVSNGERRVEVTARPAAPGEETEIWERAALVYPGYSNYAVWAGH
                     RELRVFVLEPRDGRRRE"
     gene            1556..1849
                     /locus_tag="VLS86_03510"
     CDS             1556..1849
                     /locus_tag="VLS86_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HSO49590.1"
                     /translation="MVWIDQDLCTGDGICEEITPDVYVGRDDGLWVVKEEAKYFGRTV
                     VFDAEEGEGHGPEGARGVARVPDALIEDTIESAEECPGECIMIEPYSEDAVYA"
     gene            1842..2174
                     /locus_tag="VLS86_03515"
     CDS             1842..2174
                     /locus_tag="VLS86_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSO49591.1"
                     /translation="MPELGSGSPDLRPIERVVVRLHGDGLSLPDIGKRIGKKPGTVHR
                     IIQMIDFKEDVVPARAGSVDPLRPVERVVVKLRARGESYGEIGNRLARSGRQVRMIEG
                     YADFKLGS"
     gene            2205..3116
                     /locus_tag="VLS86_03520"
     CDS             2205..3116
                     /locus_tag="VLS86_03520"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HSO49592.1"
                     /translation="MSALPSRVIITESSPRVGLQSLGAFIPTPAKAALIDDLAGAGLR
                     SFDAVSFVSPRSVPQMADGAEVVRGVDRQEGMVLLGLVPNLKGLEAAIAAGVDGIGVL
                     TAASDSFAQKNINATVDESMHRIRSILLEAPEDLATRGYISTATHCPFEGEQDPDWVA
                     HLAETLMEWGAQTVFLGETIGKATPAHVDRLLDEVLDRVPPEMVGVHFHDTYGQAIAN
                     TVVAIERGIAMMDASAGGLGGCPYAPGASGNLATEDLLWLLDGFGIEHGVDAMRVAEV
                     ASKFCSDHHLSYNSDAGRAMLAARDQK"
     gene            3113..>4075
                     /locus_tag="VLS86_03525"
     CDS             3113..>4075
                     /locus_tag="VLS86_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSO49593.1"
                     /translation="MRKDPKSIYMTDDLEAIRAQSREYVEREIVPRAQAWEQGGVPRE
                     VLDEMGRLGFFGLRIPEEHGGIGLGTMASVVFAEELGRSTFGGFSITVLVHTDLATPY
                     VLQFGTEDQKARWLPAMASGELLTAIGVTEPDAGSDVAAIRTIARRDGEDFVLNGTKM
                     FITKGGIADLVFIAAKTDSTAKGSRGISIFAVERDTPGFKASRSMDKMGWHSSNTVEL
                     TLEDARVPALNLVGEENQGFYYIMANFQNERLAIMGQSLGEAQRALELTIEHVKGRSA
                     FGSALWNKQTIRHRIAQRQTEIDAGRELTYHAAWLIDQGEDATRL"
BASE COUNT          738 a         1260 c         1374 g          703 t
ORIGIN      
        1 ggcggagtcg atgtcgatca tcagcaccgg tggcaccaga taggggtgca tctcgttcac
       61 tgcggccatg atctgctcga atcggtcgcg acgggtcttg gcgatcagca gccactcgtg
      121 gtcttcgacg atctcgtcgt cccaggtgta gacactctcg atggggatga cctgaacccc
      181 ggcagcgagc cgactctcca ccaaatgccg ggcgatcgac tgcgcttcag ccgtcgtgcc
      241 gcatgtgatc agggtcaggg cgtggcgctt cgcatcgtcc atcgaggtac tcgcgccgat
      301 gctaggaccg gtcgagcccg tgcgacgatg cggtcacgcc gacttgtagg gctggaatat
      361 ctctgtccgc caggtgctcg gatcgggctg ctcggtgggg tccgagtagt agacctccca
      421 cgccgggcca tcggggaccc cttggttctc gacgatccag gtctccaggg ccgcataagc
      481 cggcatcatc tggtcgtagg ccccgatatg tgtcacgaac gctgccttgc cgccgggcag
      541 tgtcgaggac atgacctcgc cttcgatctc cactgccctg tcgacgggaa acccggcctc
      601 gatctcgaac tggccgaact cggagtcgag aggccgcatc cgggcgaagg gcgggccggt
      661 catgaccaac cccgacttcg tcacctgatc ggcggttgcc gcgtatgcgg tgccgagaaa
      721 gaccgggatc tcatccactg cgaccttcgc cgacatcacc aacgtcggct gctcccgaag
      781 cgagcgcgag gaaacctcgt acatggtgaa cctcctttgg tccgatatcg atcatgcgcc
      841 aggagaccag aggtgcccag aggcaaaggt cccgttctgg ctcgggcttc accgaagtcc
      901 ggcgacgccc ggcgacccgg cggtctccta gcctgatcgg atgggtctcg cctcggacct
      961 cggctaccag ctcggcgacc agagccggat gcggagatgg ctggtcgaac tggccggcac
     1021 acgtctcgtg tccgccgtca atcgcaggat cctccgccct gtcgatcgcc tggctcttcg
     1081 actcaccggc gggaacaaca ccctctccac ctgggcaagc ggcatcccga ccctgtggtt
     1141 gacgacggta ggagcccgtt ccggtgagtc tcgaactgtc cccctgctcg ggatcccgat
     1201 caacgatgac ctggcggtgc tcggcaccag ctttggccag aagaagacgc cggcatgggt
     1261 gcacaacctc atggcccacc ccgatgccgt ggtctccaac ggcgagcggc gagtcgaggt
     1321 gaccgcccgg cctgccgccc ccggcgagga gaccgagatc tgggagcggg ccgctctggt
     1381 ctatcccggg tactccaact atgccgtgtg ggcgggccat cgggagctgc gggtattcgt
     1441 gctcgagccc cgggacggtc ggcgacggga atgatttggg ccgggggcgg gtagggtcat
     1501 gatgacggct gatttcgaga cgaggtgaat tggagtggcc gacaagacca agctgatggt
     1561 ttggatcgat caggacttgt gcaccggtga cggcatctgc gaagagatca ccccggacgt
     1621 gtacgtgggt cgcgacgacg ggctttgggt ggtcaaggaa gaggcgaagt acttcgggcg
     1681 aaccgtcgtc ttcgatgccg aagaaggtga gggtcacggt cccgaagggg ccaggggcgt
     1741 cgcccgtgtc cccgacgcgc tgatcgagga caccatcgag tcggcagagg aatgtcccgg
     1801 agagtgcatc atgatcgagc cctacagcga ggacgcggtg tatgcctgag ctcggctccg
     1861 gtagcccgga tctgcgcccg atcgagcggg tcgtggtcag gctccacggg gacggtctct
     1921 cgctgcctga catcgggaag cgaatcggca agaagccagg aaccgtgcat cgcatcattc
     1981 agatgatcga cttcaaagag gacgtcgtcc cggcccgagc cggaagcgtc gatccacttc
     2041 ggccggtcga gcgggtcgtc gtcaaactgc gggcccgggg cgagagttac ggcgagatcg
     2101 gcaaccgcct ggcccggagt ggccgccagg tgcggatgat cgagggttac gcagacttca
     2161 agctgggcag ctagaaaccg ctcgccctcg gttaccgtcc cggtgtgagc gctctgccct
     2221 cgcgggtcat catcactgag tcctctcctc gcgtcgggct ccagtccttg ggggccttca
     2281 tcccgacgcc ggcgaaagcc gccttgatcg acgatctggc gggggcgggg ctccgctctt
     2341 tcgacgccgt gtcatttgtc agcccgagat cggttcccca gatggcggac ggagccgaag
     2401 tggtgagagg tgtcgaccga caggagggga tggtgcttct tggcctggtc cccaatctca
     2461 agggcctcga ggctgcgatc gccgccgggg tggacgggat cggcgtgctc accgcggcgt
     2521 cggattcctt cgcccagaag aacatcaatg ccacggtgga cgagtcgatg caccggatca
     2581 ggagcatcct gctcgaggcg ccggaggatc tggccacccg cggctacatc tccaccgcca
     2641 ctcattgccc gttcgagggc gagcaggacc ccgattgggt ggcgcatctc gccgaaacgc
     2701 tcatggaatg gggagctcaa accgtcttcc tcggggagac catcggaaag gcaacgccgg
     2761 cccacgtgga tcgcctcctc gacgaagtgc tcgatcgggt gccgcctgag atggtcgggg
     2821 tgcatttcca cgacacctat ggccaggcga tcgccaacac cgtggtggcc atcgagcgcg
     2881 gcatcgcgat gatggacgcg tcggcggggg gtctcggtgg ctgcccctac gcccccgggg
     2941 cctcgggaaa cctggccacg gaggacctgc tctggctcct cgacggtttc gggatcgagc
     3001 acggcgtgga cgcgatgcgc gtcgccgagg tggccagcaa gttctgctcc gaccaccatc
     3061 tgtcgtacaa cagcgacgcc ggcagggcga tgctcgccgc cagggatcag aagtgagaaa
     3121 agacccaaag tcgatctaca tgaccgacga tctggaggcg atccgggccc agtccaggga
     3181 gtacgtcgaa cgggagatcg tgccccgggc gcaagcctgg gagcagggag gggtccctcg
     3241 ggaagtgctc gacgagatgg gcaggctcgg gtttttcggg ctgcgtatcc ccgaggagca
     3301 cggcgggatc gggctgggga ccatggcatc ggtcgtgttc gccgaggagt tgggaagatc
     3361 cacctttggc gggttcagca tcacggttct ggtccatacc gacctcgcca ctccctacgt
     3421 ccttcagttc gggaccgagg atcagaaggc acgatggctg ccggccatgg ccagcggcga
     3481 gctgctgacc gcgataggcg tcaccgagcc cgatgcgggc tccgatgtgg ctgcgattcg
     3541 aaccattgca cggagagacg gtgaagactt tgtcctcaac ggaaccaaga tgttcatcac
     3601 caagggtggt atcgccgacc tggtgttcat cgcggccaag acagactcga cagccaaggg
     3661 ctcccgtggc atctctatct tcgcagtgga gcgcgacacc ccggggttca aggcgagtcg
     3721 gtccatggac aagatggggt ggcattcgtc gaacactgtc gaattgaccc tcgaggatgc
     3781 ccgggtgccg gccctgaacc tggttggcga ggagaaccag ggcttctact acatcatggc
     3841 caacttccag aacgagaggc tggcgatcat gggccaatca ttgggagagg cgcaacgagc
     3901 cttggaactg accatcgagc atgtcaaggg ccgcagtgcc tttggatcgg cgctgtggaa
     3961 caaacagacc atcaggcacc ggatagccca gcgccagaca gagatcgacg cagggcggga
     4021 gttgacctac cacgccgcct ggctgatcga ccagggcgag gatgcgaccc gcctc
//
LOCUS       DATITW010000250         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_132233, whole genome shotgun sequence.
ACCESSION   DATITW010000250 DATITW010000000
VERSION     DATITW010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132233"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VLS86_03530"
     CDS             complement(<1..222)
                     /locus_tag="VLS86_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSO49594.1"
                     /translation="MSPIYGTVELGGTKTSFAVGTSPEDLTDIQSIPTVEPSQTLGRV
                     LDFLGAHPVEAVGIASFGPVELRQERPDYG"
     gene            443..2164
                     /gene="ilvB"
                     /locus_tag="VLS86_03535"
     CDS             443..2164
                     /gene="ilvB"
                     /locus_tag="VLS86_03535"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HSO49595.1"
                     /translation="MTKPTKFTGSHSLLRSLEHEGVEIMFGVPGGAILPAYDPLLDSP
                     IRHVLARHEQGAGHMAEGYAQATGRVGVVMVTSGPGATNLITPLQNALMDSTPLVAIT
                     GQVPTSAIGSDAFQEAYTTGLAMHCTKHSYMVTNADDIPNMVHQAFHLASTGRPGPVL
                     VDIPKDVLNQETTWTKPSLDNLPGYRPTTNGHPMQIRAAIELIEKAERPVLYVGGGVI
                     KAEASSELLAFAEAVNAPVVTTLMARGAFPDDHRLALGMPGMHGLYAATTAIQNSDVL
                     VSLGARFDDRVTGDPSAFAPNARVIHVDVDPAEIGKVRAAEVPIVGDARSVLTDLLSV
                     LRTRRDLTDSPTRAAWFEQLGSCKQDFPLSYEQSPDGPIKVQHFIERLHEMTGGQAVI
                     TAGVGQHQMWASQFWGFRDPRTWINSGGLGTMGFALPAAVGAKAARPDDLVIALDGDG
                     CFQMTCQELITASTEHIPIKVIVFNNGGYGMVKQWQNLFYGGRLSAVDLGGECPDYPK
                     LAEAMGCVGLRVDHPSEIEPAIEKALAVNDRPVVLEVVVDPEEMCFPMVPAGGSNDVI
                     VMSREDL"
     gene            2161..>2235
                     /locus_tag="VLS86_03540"
     CDS             2161..>2235
                     /locus_tag="VLS86_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HSO49596.1"
                     /translation="MMQHTLTVLVENKPGVLARVSAMFS"
BASE COUNT          426 a          743 c          690 g          376 t
ORIGIN      
        1 gccatagtcg ggccgctcct gacgcagctc gaccgggccg aacgatgcga tcccgaccgc
       61 ctccaccgga tgggcgccga ggaaatcgag cacccgcccc agcgtctgtg aaggctcgac
      121 ggtgggaatg gactggatgt cggtcagatc ctccggagag gtccccaccg caaatgaggt
      181 cttggtccct cccaattcga ctgtgccgta gatcggtgac accggccgca atctagggcc
      241 gtcggctgtg gaaaacgttg aggacgccac ggcggcgtcc gatgtagttg acatgagaaa
      301 ggccaggttg tttactagct actcgcagat gaagcagttg ttcactcccg ccgacgtact
      361 cgtacttatc gtgtagcgcg cgcagacaac cttgcgtgcg ctcaaccctc caagcccgga
      421 gggttttttg ttaccgaaca ccatgacgaa accaaccaaa ttcaccggat cccactcgct
      481 tcttcgcagc ctcgagcacg aaggggtcga gatcatgttt ggcgtgcccg gaggggcgat
      541 cctccccgcc tacgacccgc tgctcgacag cccgatacga cacgtgctgg cgcgtcatga
      601 gcagggcgcc gggcacatgg ccgagggcta tgcacaagcc accgggcggg tcggcgtggt
      661 gatggtcacc tcaggcccag gggcaaccaa cctgatcact cccctgcaga acgccctgat
      721 ggattccacc cccctggtcg cgatcaccgg gcaggtcccg accagcgcca tcggcagcga
      781 cgcgttccag gaggcctaca ccaccggtct ggccatgcat tgcaccaagc actcgtacat
      841 ggtgaccaat gccgacgaca tcccgaacat ggttcatcag gccttccatc tcgcctccac
      901 cggccgtccc ggcccggtcc ttgtcgacat ccccaaggac gtactgaacc aggagaccac
      961 ctggaccaaa ccgtctcttg acaacctgcc cggctaccgg ccgaccacca acggtcatcc
     1021 gatgcagatc cgagctgcca tcgagttgat cgagaaggcc gagcgcccgg ttctgtatgt
     1081 cggtgggggt gtgatcaagg ccgaagcttc cagcgagctg ctcgctttcg ccgaagcggt
     1141 caacgccccg gtggtgacca cgttgatggc gcggggcgca ttccccgacg atcaccggct
     1201 ggcactgggc atgcccggca tgcacggctt gtacgcggcc acaaccgcga tccagaactc
     1261 agacgtcctg gtgagtctgg gcgcccgctt cgacgacagg gtcaccggcg atcccagcgc
     1321 attcgccccc aatgccaggg tcattcacgt cgacgtcgac ccggccgaga tcggcaaggt
     1381 gcgggcggcc gaggtcccca tcgtcgggga cgcgagatcg gtactgaccg atctgctctc
     1441 ggtactccgc accaggcgcg acctcacgga ctcgccgacc agagccgcat ggttcgagca
     1501 gttgggcagc tgtaagcagg atttcccgct gtcctacgaa cagagccccg acggcccgat
     1561 caaggtccag cacttcatcg agcggctgca cgagatgacc ggcgggcaag ccgtcatcac
     1621 cgccggcgtc ggtcaacatc agatgtgggc gtcgcaattc tggggattcc gcgatccccg
     1681 gacctggatc aactccgggg gcctggggac gatgggcttc gccctcccgg cagcggtcgg
     1741 ggccaaggcg gctcgacccg acgacctggt catcgcactc gatggcgacg gctgtttcca
     1801 gatgacctgc caggagctga taactgcgag caccgagcac atcccgatca aggtcatcgt
     1861 gttcaacaac ggtggctacg ggatggtcaa gcagtggcag aacttgttct acggcggccg
     1921 gctctccgcc gtcgacctgg gtggcgaatg cccggactat ccgaagctgg ccgaggcgat
     1981 gggatgcgtc gggctcagag tcgatcaccc atcggagatc gagccggcca tcgagaaggc
     2041 actggcggtc aacgaccggc ccgtggtgct cgaagtcgtg gtcgaccccg aggaaatgtg
     2101 cttcccgatg gtcccggcgg gtggatcgaa cgacgtcatc gtcatgagcc gcgaagacct
     2161 ctgatgcagc acaccctcac cgtcctcgtg gagaacaagc caggagtgct cgcccgggtg
     2221 tcggcgatgt tctcc
//
LOCUS       DATITW010000251         1626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_212636, whole genome shotgun sequence.
ACCESSION   DATITW010000251 DATITW010000000
VERSION     DATITW010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212636"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1626
                     /locus_tag="VLS86_03545"
     CDS             <1..>1626
                     /locus_tag="VLS86_03545"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSO49597.1"
                     /translation="AWSRRGETLLEGEPESYAHGYLKLAQASEAPDAAAALELFEEAA
                     EIALRTNTPDLRALALSRGGYLKVNTGAVKEGFAMLEESAIAAVNGELSPLVAGISCC
                     NMISCCRDLTDYRRAREWIEATEKFCERASVAGFPGICRVHKAEVVALTGAWAKAEED
                     LRKATTELAAYNAIPPMGDGYYAIGEIRLRMGDFEGAEEALRSAHAIGKSPQPALARI
                     RLAQGKVKQAETAINSALAENPWDRAGRTRLLPAAVEIFVASGALSAARDAAQEMERL
                     VEDFDSPAMQAARHDSWGRVLLAEGDAAGASTHLRDAVATWRKVGAPFEVARARVLLS
                     MALRATRDEEGADLELATARDEFWKLGAEWATAEADKTIQSINERRSRPVQARKTFMF
                     TDIVGSTNLAELLGNEGWDQLLKWHDQALRSQFSRSGGEVVNSTGDGFFVAFESARQG
                     IDCAIAIQQTLADHRRTTGFAPSVRIGLHLAEANRQGDDYTGKGVHVAARVAALGGAG
                     EIIASVETLEEAGDVAATASREATLKGVAAPVLV"
BASE COUNT          298 a          493 c          567 g          268 t
ORIGIN      
        1 gcctggtctc gccgtggtga gacgctcctc gagggcgagc ccgagagcta cgcccacggc
       61 tacttgaagc tcgcacaagc ttccgaagct ccggacgccg ccgcagcgct cgagcttttc
      121 gaggaggctg ccgagatcgc gttgcgaacc aacacgcccg atctgcgtgc gctggctttg
      181 agccgcggcg gttacttgaa agtcaacacc ggggctgtca aagagggctt tgcgatgctg
      241 gaggagtcgg caattgctgc ggtcaacggt gagctctcgc cgcttgtggc ggggatcagt
      301 tgctgcaaca tgatctcttg ctgccgcgac ctcacggatt accggcgtgc tcgggaatgg
      361 atcgaggcaa cggagaagtt ctgcgagcga gcatctgtgg ccggctttcc ggggatttgc
      421 cgggtgcaca aggccgaggt cgtcgctctc accggggcgt gggccaaagc cgaagaagac
      481 ctgcgcaaag ccacaaccga gctcgctgcc tacaacgcca ttcctccgat gggagacggc
      541 tactacgcaa tcggcgagat ccggttgcgg atgggcgact tcgagggagc ggaggaagcc
      601 ctgcggagtg ctcacgccat cggaaagagc ccacagccgg ccctggcccg gatccgcctg
      661 gcccagggga aggtgaagca ggcggaaacc gccatcaact cggctctggc ggagaacccc
      721 tgggaccggg ccggcaggac ccggctcttg cccgcagccg tcgagatctt cgttgcatcg
      781 ggcgccctct cagcggcgcg ggatgcggct caggagatgg agcgcctggt ggaagatttc
      841 gattcgccgg ccatgcaggc cgctcgccac gattcatggg gtcgggtcct cctggccgaa
      901 ggagacgctg ccggcgccag cacccacctg cgagatgcgg tcgccacctg gagaaaggtt
      961 ggcgcacctt ttgaggttgc ccgggctcgg gtactgctgt cgatggcact tcgggccacc
     1021 cgggacgagg aaggagccga cctcgaactc gccaccgccc gcgatgagtt ctggaaattg
     1081 ggagcggagt gggcgacggc agaggctgac aagaccatcc aatcaatcaa tgagcggcgg
     1141 tcacggccgg tccaggctcg gaagaccttc atgttcacgg atatcgtcgg ctcgaccaat
     1201 ctcgccgaat tgctggggaa cgaagggtgg gatcagttgc tcaagtggca tgaccaggct
     1261 cttcgatctc agttcagcag gagtggcggc gaggtggtca actcgacggg cgatgggttc
     1321 ttcgtcgcgt tcgagtcggc ccggcaagga atcgattgtg cgatagcgat tcaacagacc
     1381 ctggccgatc atcggcggac tacggggttc gccccgtcgg tccgcatcgg cctccacctg
     1441 gccgaagcca accggcaggg cgacgactac accggcaaag gtgtccacgt ggctgcccgc
     1501 gtggcagcgt tgggaggggc cggggagatc atcgcgagcg tcgaaacgct cgaggaagcc
     1561 ggtgacgtgg cggcgaccgc gagccgcgaa gccaccctca aaggggtggc cgcccctgtt
     1621 ctggtg
//
LOCUS       DATITW010000252         3238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_12491, whole genome shotgun sequence.
ACCESSION   DATITW010000252 DATITW010000000
VERSION     DATITW010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12491"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VLS86_03550"
     CDS             <1..456
                     /locus_tag="VLS86_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HSO49598.1"
                     /translation="GGKPGDKLLVQDVIADAMLQQVLTRPASYEVIATTNLNGDYLSD
                     ALAAQVGGIGIAPGGNINYVSGHAVFEATHGTAPKYSGLDKVNPGSLILSGEMMLRYM
                     DWGEAADRIITGLEGAIAARTVTYDFARLSDDATEVKTSEFGDAIIAHM"
     gene            complement(460..1209)
                     /locus_tag="VLS86_03555"
     CDS             complement(460..1209)
                     /locus_tag="VLS86_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HSO49599.1"
                     /translation="MANPVLEKQFGTASADVALQTAQPPPATGADTMSMGSVITSTLV
                     LLILVMGGAAFGWANAAAVQRWYWAFIIGLIVLVIATVARPQLAPITGIIYSLGQGAM
                     LGSISQVYETCYDGIVLQAFLATMAVFVSMLFLYATRIVKVTQKLRSVIIIATVGIAL
                     FYLFSWILTLFNVDVPVLSGAGTPALIFSILVVFVAALNLLLDFQVIEIVVRNCAPRS
                     FSWFAAFGLMVTIVWLYIEMLRLLAILARNR"
     gene            1305..2273
                     /gene="nadA"
                     /locus_tag="VLS86_03560"
     CDS             1305..2273
                     /gene="nadA"
                     /locus_tag="VLS86_03560"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HSO49600.1"
                     /translation="MTPIAITRAADVLHEVEVAALQPLIDEINWWKKERNAIVAAHNY
                     MTPDIFHLAADITGDSLALAQIAVDTEADVIVLAGVHFLAETAKIINPEKMVLIPDLR
                     AGCSLADGITGADVRALREQHPGVPVVTYVNTSAEVKAESDITCTSSNAVAVVESLGV
                     DKVIFTPDQYLGMWVATQTDVEVILWEGSCIVHERFTGEEIAEYRRGANGIKVIAHPE
                     CPPDVLAEADYVGSTHGMIQWVTTNQPDRVMMITECSMADNVASQTPNTEYVRPCNLC
                     PHMKRITLHNIRDSLVTLTHQIEIDPAIAERARRADFRMLEVGRQD"
     gene            2283..>3238
                     /locus_tag="VLS86_03565"
     CDS             2283..>3238
                     /locus_tag="VLS86_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSO49601.1"
                     /translation="MTEISRLDAPLIVGAGVAGLTVALGLPRAYVMSAPEMGSTWWAQ
                     GGIAVALSDEDSPELHADDTFNVSGGLAVRNVVDVLTAGGPAAIDNLIRLGAEFDRDD
                     DGGLSLGQEGGHQRRRVVHADGDATGAEVMRALMAAVKATDRIEVIESRVVDLVMADS
                     RVVGVVTADGKQRDVILAPAVVLATGGAGRLYSRTTNPPGVTGDGIAMAGRVGARLAD
                     LEFVQFHPTALLAGKDPMPLLTEALRGEGAILVDAHGRRFMDQYHPMAELAPRDIVAR
                     AIFWQYDKGMGAYLDARSLVNFADRFPTVNAHAHSVGLDPEQ"
BASE COUNT          634 a         1007 c         1043 g          554 t
ORIGIN      
        1 ggggggaagc cgggcgacaa gctcctggtc caggacgtca ttgccgatgc catgctgcaa
       61 caggtcctca cccggcccgc cagctatgag gtgatcgcca ccaccaacct caacggtgac
      121 tatctctcag atgcgctcgc cgcccaggtc ggagggatcg ggatcgcccc cggtggcaat
      181 atcaattacg tctctggcca tgccgtgttc gaagcgaccc atgggaccgc tcccaaatac
      241 tcaggtctcg acaaagtgaa cccgggctcc ctcatcctgt ccggtgagat gatgctccgc
      301 tatatggatt ggggtgaggc ggccgaccgg atcatcaccg gtctggaggg cgcaattgca
      361 gcccgcaccg tcacctatga cttcgcccgg ctcagcgacg acgcgaccga ggtcaagacc
      421 tcggagttcg gtgacgccat catcgctcac atgtagtttt cagcggttgc gggcgaggat
      481 cgccaacaac cgcagcatct cgatgtagag ccagacgatc gtcaccatca gcccgaacgc
      541 ggcgaaccaa gagaacgatc gcggtgcaca gttccgtact acaatttcga tcacctgaaa
      601 gtcgaggagg aggttgaggg cggcgacaaa caccacgagg atgctgaaga tcagggctgg
      661 cgtgccagct ccgctgagaa ccgggacgtc gacgttgaac aaggtcagga tccagctgaa
      721 cagatagaag agagcgatcc cgaccgtggc gatgatgatc accgagcgca gcttctgggt
      781 gaccttgacg atgcgggtgg catagaggaa gagcatcgag acgaagaccg ccatcgtcgc
      841 cagaaacgcc tgtaacacga ttccgtcgta gcaggtctcg tagacttggg agatcgaacc
      901 gagcatcgcg ccctgtccga gggagtagat gatcccagtg atcggcgcga gttggggtcg
      961 tgccactgtt gcgatcacca ggacgatgag gccgatgatg aaagcccaat accagcgttg
     1021 gacggctgcc gcgttggccc aaccaaaagc ggcgcccccc atcaccagga tcagcaatac
     1081 cagggtggac gtgatgaccg agcccattga catcgtgtcg gcgccggtcg ccggcggtgg
     1141 ttgggcggtc tgcaatgcga catccgcgct ggccgtgccg aactgcttct caagaacagg
     1201 gtttgccatg gaccggagtg tatttccggg gtcgccggcc tgtggttgcc tccaagcagt
     1261 ggaccgggga acacgccggc ggctggtctt acactcattc cctgatgacg ccgatagcaa
     1321 tcacccgcgc tgcggatgtg cttcatgagg tcgaggtcgc cgcgcttcag ccgttgatcg
     1381 acgagatcaa ttggtggaag aaggagcgca acgccatcgt cgccgcccac aactacatga
     1441 cgccggacat cttccacctg gctgccgaca tcaccggcga ctcgctggcc ctggcccaga
     1501 ttgcggtgga caccgaggcc gacgtcatcg tgctcgccgg ggttcatttc ctggcggaga
     1561 ccgccaagat catcaacccg gagaagatgg tgctgatccc cgacctccgg gcggggtgtt
     1621 cgttggccga cgggatcacc ggagccgacg tgagggcgct gagggagcag caccctggcg
     1681 tccccgtggt gacctatgtc aacacctctg ccgaggtcaa agccgagtcg gacatcacct
     1741 gcacgtcgag caacgccgtc gctgtcgtcg aatcgttggg tgtcgacaag gtgatcttca
     1801 ccccggacca gtacctcggg atgtgggtcg ccacccagac cgatgtcgag gtgatcctct
     1861 gggagggatc gtgcatcgtc cacgagcgat tcaccggtga ggagatcgcc gaataccggc
     1921 ggggggcgaa cgggatcaaa gtcatcgccc accccgagtg cccgccggat gtcctcgccg
     1981 aggccgatta cgtcgggtcg acacacggga tgatccagtg ggtgaccacc aaccaacccg
     2041 accgggtgat gatgatcacc gagtgctcga tggcggacaa cgtcgccagc cagaccccga
     2101 acaccgagta cgtgcggccc tgcaacctct gtcctcatat gaagcggatc accttgcaca
     2161 acatcaggga ctcactggtc acgctcaccc accagatcga gatcgatccc gccatcgccg
     2221 agcgagcacg gagggccgat ttccggatgc tcgaggtcgg tcgtcaggat taggactttc
     2281 agatgacgga gatctcgcgc ctcgacgccc ctttgatcgt cggtgctgga gtggccgggc
     2341 tcaccgtggc cctcggcctt ccccgggcct acgtgatgtc ggctcccgag atgggctcca
     2401 cctggtgggc ccagggcgga atcgccgttg ccctttccga tgaggactcg cccgagttgc
     2461 acgccgacga caccttcaat gtttcgggtg gcctggcagt gagaaacgtg gtcgacgtcc
     2521 tgaccgcggg cggcccggcc gcgatcgaca atctgatcag actgggcgcc gagttcgatc
     2581 gcgatgacga cgggggtctc tcactgggcc aggagggcgg ccatcagcgg aggagggtcg
     2641 tccacgccga cggagacgcc acgggagcgg aagtgatgcg ggccttgatg gcagcagtca
     2701 aggcaaccga ccggatcgag gtcatcgaga gcagggtcgt cgatttggtg atggccgaca
     2761 gccgggtcgt cggggtggta actgcggacg gcaagcaacg cgatgtgatc ctggccccgg
     2821 cggtggtgct ggcgacaggt ggcgcgggcc gtctctactc acgaaccacc aacccaccag
     2881 gggtcaccgg agacggaatc gcgatggcgg gccgggtcgg ggcccggttg gccgatctcg
     2941 agttcgtcca gttccatccc accgccctcc tcgccggcaa ggacccgatg cctctgctca
     3001 ccgaggcgct gaggggcgag ggggcgatcc tggtcgatgc ccatggccgt cgtttcatgg
     3061 accaatacca tccgatggcc gaactggcac cccgcgacat tgtcgctagg gcgattttct
     3121 ggcagtacga caaggggatg ggcgcctatc tcgatgcccg gtccttggtg aatttcgccg
     3181 accgcttccc gacagtcaac gcccacgccc attcggtcgg cctcgacccc gagcagga
//
LOCUS       DATITW010000253         1623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_292837, whole genome shotgun sequence.
ACCESSION   DATITW010000253 DATITW010000000
VERSION     DATITW010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292837"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1623
                     /locus_tag="VLS86_03570"
     CDS             <1..>1623
                     /locus_tag="VLS86_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSO49602.1"
                     /translation="VIGAGSFIADLIDESTLHCAFVRSPVAHGTFDPPPLDDALAMPG
                     VVAVYRADSLGLPDLPSSPDLGAPEAVGMGQPPLARDRVRHVGDPIAVVVAETARQAV
                     DAAEQIWVDIEPLPSVPDVTAALRDETLLFPKVGTNLVEEATIRSPGTAPSYEHETEI
                     EVEIPRISPVTIEPLAILVRPTADGLDVWCGHQSPGRLPNQLGETLGLPPSMIRARVP
                     DVGGAFGTKRPFYAEYAVVTAIAYRLSRPVVWIQGRREQLISGTHGRGQTIKVRIGGD
                     ADGRIRGVHIEIAGDLGAYPSTGSRVPFFTQYMGQGQYDIDYVETHAVVAVTNKAPTG
                     PYRGAGRPEAAIAIERAVDAFAVEIGMRPEEVRRRNYIPSSRLPFTTVTGAIYDSGDY
                     AAALELALETVGMEQWRTEQERRRSEGSNPIGIGIGSFIERAGGAVGSAEYGKVELLP
                     DGTVVVRTGSTAAGQGHRTVWSQIAASVFSVPLDRITFYAGDTQEVANSVGSFGSRSA
                     QLGGSAVFRTATVVRDRAQKLAAEMLEAAEAD"
BASE COUNT          273 a          541 c          548 g          261 t
ORIGIN      
        1 tcgtcatcgg cgccggctcg ttcatcgccg acctgatcga cgagagcaca ctgcattgtg
       61 ccttcgtgcg aagccctgtc gcccacggca cattcgatcc cccacctctc gacgacgccc
      121 tcgccatgcc aggtgttgtc gctgtctacc gcgccgacag cctcggtctt cccgacctcc
      181 catcctcccc cgatttggga gcacccgagg cggtgggcat gggtcagccg cccctggcca
      241 gggaccgggt gcgacatgtg ggcgatccga tcgcggtggt ggtcgcggag accgcccgac
      301 aggcggtgga cgccgccgag cagatctggg tcgacatcga gccgctgcct tcggtcccgg
      361 acgtgaccgc ggcactgcgc gacgagacgc tcctgttccc caaagtcggc accaacctgg
      421 tcgaggaggc aacgatccgc agcccgggga ccgcgcctag ctacgagcac gagaccgaga
      481 tcgaggtaga gatcccccgc atctccccgg tgaccatcga gccgctggcg atcctggtcc
      541 gaccgactgc cgacgggctc gacgtctggt gcggtcacca gtcaccgggg cgccttccca
      601 accagttggg cgagactctg gggttgccgc cctcgatgat cagggctcgg gttcccgatg
      661 tcgggggggc ctttgggacc aagaggccgt tctatgccga gtatgccgtt gtcaccgcga
      721 tcgcatatcg attgagcaga cccgtggtct ggatccaggg gcgcagggag caactcatct
      781 ccggcaccca cggaagggga cagacgatca aggtccggat cggtggcgat gccgacggcc
      841 ggatccgggg cgtgcacatc gagatcgccg gtgacctcgg cgcctacccg agcaccgggt
      901 cgagggttcc gttcttcacc caatacatgg gtcaggggca atacgacatc gactacgtcg
      961 aaacccacgc cgtggtcgcc gtgaccaaca aggcccctac cggaccctat cggggcgcgg
     1021 gaagaccgga ggcggccata gccatcgaga gggccgtcga cgcctttgcc gtcgagatcg
     1081 gcatgagacc tgaggaagtt cgtcgcagga actacatccc ctcgtcgcgg cttccgttca
     1141 ccacggtcac cggggcgatc tacgactccg gtgactacgc cgctgccctc gagctggcgt
     1201 tggagaccgt ggggatggag cagtggcgca ccgagcagga acgacgtcgg agcgaagggt
     1261 cgaacccgat cgggatcggc atcggctctt tcatcgagcg agccgggggg gcagtcggct
     1321 cggcagagta cggcaaggtc gagttgctcc ctgacgggac cgtggtcgtc aggaccggct
     1381 cgaccgcagc cggtcagggg caccggacgg tctggtctca gatcgcagcc agtgtcttct
     1441 ccgtccctct cgatcggatc acgttctacg ccggcgacac ccaggaggtg gccaacagcg
     1501 ttggcagctt cgggagcagg tcggctcaac tcgggggctc ggcagtgttc cggactgcca
     1561 ccgtggttcg cgaccgggct cagaagctgg ccgcggagat gctcgaggcg gccgaggccg
     1621 acc
//
LOCUS       DATITW010000254         3726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_292842, whole genome shotgun sequence.
ACCESSION   DATITW010000254 DATITW010000000
VERSION     DATITW010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292842"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VLS86_03575"
     CDS             <1..957
                     /locus_tag="VLS86_03575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HSO49603.1"
                     /translation="HIHRVQQIANAGIKDGRKIAFFGRSMHKNTTIAVDLGVLEVPAK
                     SVIDIKELSQLPPEQQLLITTGSQGEPFAALSLISQGRHKFVELGEGDTVLISAKPIP
                     GNETAVSKVISNLTRRGANVVHGQNANVHVSGHAARDELMTFLNVVRPDAFVPVHGEY
                     RHLHAHAELARRMGVGHVDVLEDGDRVIINGKKTMVERRAVPAGYVYLDGSGVGDVQS
                     AVLRDRSHLADEGVVVVTVGIDHDTGDILYGPEMDSHGVMDDPTSVLAKVADAVTEAI
                     DSHASGRSDIGELQKTIRQAAGRVIRAETSRRPVIVPVVMEL"
     gene            1052..1540
                     /locus_tag="VLS86_03580"
     CDS             1052..1540
                     /locus_tag="VLS86_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49604.1"
                     /translation="MTTIWRVIHIFSGVLWMGSFAVFTLFISPSARKLGDEARPFMQA
                     LLKTRLIESLLSVGVATVVSGLFLYAGRFGEVELSTRSGLWLTVGGLAGITALLVGST
                     FGARSNLRMRALAAEIAGSGGPPSAGQTAEMQRLQARVVRIGTPMLVLFAIALLGMAL
                     GG"
     gene            1637..>3726
                     /locus_tag="VLS86_03585"
     CDS             1637..>3726
                     /locus_tag="VLS86_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HSO49605.1"
                     /translation="MSTAKKKTAPKATFRQKLVAVRSSVRGTLGRQTDDVWGLVLLVL
                     AVLVLLAFLDLAGPVGHGMAGGSLFLFGVWRFAVPVALAGIGIALILGKPKDGARRLV
                     AGAVVTFVGSLALFHLMTGAVALAPNIDLVKERGGAVGSLLAFPLRRILGTWGAFVVL
                     ASAMGMGVLVMTQTSVRELALGISDLFKSTRRLATSAFVGGVAAQGAHVSDRVARPAQ
                     AERGKHERPKQSRPRPEKPRETPKPAPRPMPVVKTVDLTNSGYRLPPLSILDEGTGGE
                     VNKRALEDTARQLEETLIQHGVDATLTKIVPGPTVTRYEIELAPGVKVNRVSSLSHDI
                     AYALATPDVRILVPIPGKSAIGIEVPNTKRRLVSLGDILSSAEAALASHPLTVGLGMD
                     ISGESKLLNLAELPHVLIAGATGAGKSSCINSIVTSLLMRTHPDDVRLIMVDPKRVEL
                     GQYNGVPHLLTRVITNPKKANDALKWAVAEMDRRYDLLADAKVRDIEGYREKWEAGGL
                     DRDGFDRFPYIVILIDELNDLMMFAGREVEESIVRIAQMARAVGIHLVIATQRPSVDV
                     ITGVIKANVPSRLAFSVASQTDSRVILDGSGAEKLVGMGDMLVVTAKEPRAQRIQGAW
                     VRESEIQAVVDWVRLQKEAVYQDQEVLEVVAAAAAAQAEEEDDEDAALAREAMEMVVR
                     SQLGSTSMLQRKLR"
BASE COUNT          628 a         1216 c         1255 g          627 t
ORIGIN      
        1 catatccacc gcgtgcagca gatagccaac gccgggatca aggatgggcg aaagatcgcc
       61 ttcttcgggc ggtcgatgca caagaacacc acgatcgccg tcgacctggg tgtgctcgag
      121 gtcccggcca agtcggtgat cgacatcaag gagctgagcc agctgccacc agaacaacag
      181 cttctgatca ccaccggcag ccagggtgag ccgtttgccg ccctttcgct catctcccag
      241 ggcaggcaca agttcgtcga gctcggcgag ggggacacgg tcctgatctc ggccaagccg
      301 atacccggca acgagaccgc ggtctccaag gtgatctcga atctgacccg gcgcggggcg
      361 aatgtggtgc acggtcagaa cgccaacgtc catgtctccg gtcacgccgc ccgcgacgag
      421 ctgatgacgt ttctcaacgt ggtccgtccc gatgccttcg tcccggtcca tggggagtac
      481 cgccaccttc atgcccacgc cgagctggca cgccgcatgg gtgtcggtca cgtcgacgtc
      541 ctcgaggacg gggaccgcgt gatcatcaac ggcaagaaga ccatggtcga gagacgggcg
      601 gtgccggccg gatacgtcta cctcgacggg tccggggtgg gcgacgtcca atcggcggtg
      661 cttcgtgacc ggtcgcatct cgcggacgag ggtgtggtgg tggtgaccgt cgggatcgac
      721 cacgacaccg gggacatcct ctacgggccg gagatggaca gtcacggggt gatggacgat
      781 cccacctcag tgctggccaa ggtggccgac gccgtgaccg aggcgatcga ttcccatgca
      841 tccgggcgat ccgacatcgg cgagctgcag aagacgattc gccaggcggc gggtcgtgtg
      901 atcagggccg agacctcacg tcgcccggtg atcgtccccg tggtcatgga gctctagccg
      961 gggcccggaa acttgcgcgc cttttgggcg caggacgtgg tttcacgtcg tagcgttggc
     1021 cggcaggaga ccgagaaagc gaggacatcc ggtgaccacc atctggcgtg tgatccacat
     1081 cttctccggg gtgctgtgga tgggcagctt cgccgtcttc accctgttca tctcaccgtc
     1141 cgcccggaag ctgggcgatg aggcccgccc gttcatgcag gcgctgctca agacgaggct
     1201 gatcgagagc ctgctctcgg tgggggtggc cacggtggtc tccgggctct tcctctacgc
     1261 cggccgattc ggcgaggtcg agctctcgac ccggtccggg ctctggctga ccgtgggcgg
     1321 tctggcgggc atcaccgcac ttctcgtcgg ctccacgttc ggggctcgaa gcaatctccg
     1381 gatgagggcg ctggccgcgg agatcgcggg gagcgggggg ccgccgagcg cggggcagac
     1441 ggcagagatg cagcgtctcc aggcccgtgt cgtccgcatc gggactccga tgctcgtctt
     1501 gtttgcgatc gccctgctcg ggatggcgct cggcggctga ccagcaatcc acgtccaaat
     1561 ccgtctccca tgtattgttt actgacacat gggatcgagg acgcccaccc ggaaggggcc
     1621 ctccgggcgg aggcgggtgt ccacagccaa gaaaaagaca gcgcccaagg ccacgttccg
     1681 ccagaagctg gtggcggtcc gcagctcggt gcgcggcacg ctcggccgcc agaccgatga
     1741 cgtctggggt ctcgtgctcc tcgtcctcgc cgttctggtc ctgctcgcct ttctcgatct
     1801 cgccggcccg gtcggtcacg gcatggccgg cgggagcctc ttcttgttcg gcgtgtggcg
     1861 gtttgcggtg ccggttgccc tggccggcat cggcatcgca ttgatactgg gcaagcccaa
     1921 ggatggggcg cgacgtctcg tcgccggcgc cgtggtcacc ttcgtcggct cgctcgccct
     1981 gttccacctg atgacgggag cggtggccct cgccccgaac atcgacctgg tcaaagagcg
     2041 cgggggagcc gtcgggtcgc ttctcgcttt cccgctgcgt cggatcctcg gcacctgggg
     2101 ggcgtttgtc gtcctcgcct cggccatggg aatgggggtg ctggtcatga cccagaccag
     2161 tgtccgcgag ctcgccctgg ggatcagcga tctcttcaag tcgacccgca gactcgccac
     2221 atcggcgttc gtcggcggag tcgccgccca gggcgctcat gtctcggacc gggtggcgcg
     2281 accggctcag gcagaacgcg gcaagcacga gcgacccaag cagtcccggc cccggcccga
     2341 gaagccgcgg gagacaccaa agcctgcacc ccggccgatg cccgtggtga agaccgtcga
     2401 cttgaccaac agcggctacc ggctgccccc gctgagcatc cttgacgagg ggaccggggg
     2461 tgaggtcaac aaacgcgctc tcgaagacac cgcccgccag ctggaggaga ccctgatcca
     2521 gcatggcgtc gatgccaccc tgaccaagat cgtccccggg cccaccgtca cccgctatga
     2581 gatcgagttg gcgccagggg tgaaggtgaa ccgggtctcc agtctctccc acgacatcgc
     2641 ctatgccctg gccacgcccg atgtgcgcat cctggtcccc atcccgggga agtcggcaat
     2701 aggcatcgaa gtgccgaaca ccaagcggcg tctggtctcg ctcggagaca ttctgtccag
     2761 cgcagaggcg gccctggcca gccacccgct gacggtgggc ctggggatgg acatctcggg
     2821 ggagtcgaag ctgctcaacc tggccgagct ccctcacgtc ctgatcgccg gcgccaccgg
     2881 cgccggcaag tcgtcgtgca tcaactcgat cgtcacctca ctgttgatga gaacccaccc
     2941 cgacgacgtg cggctgatca tggtcgatcc caagcgcgtc gagctcggcc aatacaacgg
     3001 ggtgccccat ctcctgaccc gggtcatcac caatcccaag aaggccaatg acgccttgaa
     3061 gtgggcggtc gccgagatgg atcgccgcta tgacctgctc gccgacgcca aggtccgcga
     3121 catcgagggc taccgggaga agtgggaggc cggcgggctc gaccgggatg gattcgaccg
     3181 cttcccttac atcgtgatcc tgatcgacga gctcaacgac ctgatgatgt ttgccgggcg
     3241 agaggtggaa gagtcgatcg tgcgcatcgc ccaaatggcg cgggcggtcg ggatccatct
     3301 ggtcattgcc acccagcggc cttcggtcga cgtgatcacc ggcgtgatca aggccaacgt
     3361 gccgagccgt ctcgcattca gcgttgcctc tcagaccgac agccgggtca tcctcgacgg
     3421 ctccggtgcc gagaagctgg tggggatggg cgacatgctg gtggtgaccg ccaaggagcc
     3481 tcgtgcccaa cggatccagg gtgcctgggt ccgagagagc gagatccaag ccgtcgtcga
     3541 ctgggtcagg ctgcagaagg aggccgtcta tcaagaccag gaggtcctgg aggtagtggc
     3601 tgccgccgcc gcggctcaag ccgaggagga ggacgacgaa gatgcggcgc tggcccggga
     3661 ggccatggaa atggtcgtcc ggagtcaact cggctcgacc tcgatgctgc agcgcaagct
     3721 ccggat
//
LOCUS       DATITW010000255         1846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_172489, whole genome shotgun sequence.
ACCESSION   DATITW010000255 DATITW010000000
VERSION     DATITW010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172489"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VLS86_03590"
     CDS             <1..534
                     /locus_tag="VLS86_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daunorubicin/doxorubicin resistance ABC
                     transporter ATP-binding protein DrrA"
                     /protein_id="HSO49606.1"
                     /translation="FLDEPSSGLDPRSRTELWDVIRELVARGTTLLLTTQYLEEADQL
                     ADDIVVIDHGREIAHGTSDQLKIQVGGERIEVSVREAADVAVAREVLTPIAVGEIQVD
                     ERSRALTAPISGGATVLTQALRDLDARGVVVQDVGLRRPTLDDVFLSLTGHVAEAAAS
                     NGGEPEARKAEHEEATR"
     gene            531..1334
                     /locus_tag="VLS86_03595"
     CDS             531..1334
                     /locus_tag="VLS86_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49607.1"
                     /translation="MTQLMTALADGAVVAKRNVIKIKRVPEILVFVLLSPIMFVLLFA
                     FVFGGAIDVAGGSYREFLIAGILAQTVIFGATFTGAGLADDMQKGIIDRFRSLPMARS
                     AVLVGRTASDIIYNALSITIMSLTGLLVGWGISTSVAEAALGFGLLLLFSYAFSWVMA
                     YVGLLIPSVEVINNASFMVIFPITFVSNAFVPIETLPGPLQTFAEWNPVSALTQAVRE
                     LFGNIPPGAPEPTAWPLQNPILYTLIWVAILLAIFVPISVRQYKRAARR"
     gene            1383..>1846
                     /locus_tag="VLS86_03600"
     CDS             1383..>1846
                     /locus_tag="VLS86_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSO49608.1"
                     /translation="MKPAQSTAEVILAAITAAVGAGRSLKDVLHGLAEAARDISSASY
                     AAIGIPEPDGDAFSAFIHVGMKDELVAEMGPLPRTHGMLDAMLHDPEPVRLADITKDP
                     RFRGWWPRAHPLMRSFLGVPIVAQDEVIGAFYLTDKIGAAVFTAADQANIGLL"
BASE COUNT          298 a          614 c          597 g          337 t
ORIGIN      
        1 ttcctcgacg agccgtcgtc cgggctcgac ccccgcagcc gaaccgagct ctgggacgtc
       61 atccgcgagc tggtggcccg gggcacgacg ctgctcctga ccacccagta cctcgaggag
      121 gccgaccaac tcgccgacga catcgtggtg atcgaccacg gtcgtgagat cgcgcacggg
      181 acctccgacc agctcaagat ccaggtgggt ggcgagcgga tcgaggtcag tgtcagagag
      241 gcagccgatg tggcggtcgc ccgtgaggtg ctgacgccga tcgcggtcgg cgagatccag
      301 gtcgacgagc gatccagggc cttgaccgcc ccgatcagcg gtggggccac cgtcctgacc
      361 caagcgctgc gcgacctcga tgcacggggc gtcgtcgtcc aggatgtcgg tctccgccgg
      421 cccaccctcg acgacgtctt cctgtccctg accggtcacg tcgccgaagc ggctgcttcc
      481 aacggtggcg agcccgaggc tcgaaaagca gagcatgagg aggcaactcg atgacacagc
      541 taatgacagc acttgccgac ggcgcggtgg tcgccaagcg caacgtgatc aagatcaagc
      601 gagtgccgga gatcctggtc ttcgtgctgc tgtcaccgat catgttcgtg ctcctcttcg
      661 ccttcgtgtt cggtggtgcg atcgacgtgg cgggcggctc ctaccgcgag ttcctcatcg
      721 ccgggatcct ggcccagacc gtgatcttcg gagccacctt caccggtgcc gggttggccg
      781 acgacatgca gaaggggatc atcgatcggt tccgatcgct gcccatggcg aggtcggcgg
      841 tgctggtggg aaggaccgcg agcgacatca tttacaacgc cctttcgatc acgatcatgt
      901 cgctgaccgg actgctggtc gggtggggca tcagtacgtc ggtcgccgag gcggctctgg
      961 gattcgggct tctcctgctc ttctcctacg ccttctcatg ggtgatggcc tacgtgggct
     1021 tgctcatccc gagcgtcgag gtgatcaaca atgcctcgtt catggtgatc ttcccgatca
     1081 cgttcgtctc caacgcgttc gtgccgatcg agacacttcc agggccgctg cagacgttcg
     1141 ccgagtggaa tccggtgtcg gctctgaccc aggcagtgcg cgagttgttc gggaacatcc
     1201 caccgggagc gcccgagccc accgcatggc ctctgcagaa cccgatcctg tacaccctga
     1261 tctgggtggc catcctcctc gccatcttcg tgcccatctc ggttcgccag tacaagaggg
     1321 ctgcccgccg ctgagcccgt cttctcagta ggggagtccg ggttcgtcgg tagccttcgc
     1381 gggtgaagcc agcccagagc accgccgagg tgatcctggc cgcgatcacc gcggcggtgg
     1441 gagccggtcg gtccctgaaa gacgtccttc acgggctggc ggaggcggcc cgggacatct
     1501 ccagtgcctc ctatgcggca atcggcatcc ccgagcccga cggtgacgcc ttctccgcct
     1561 tcatccacgt cgggatgaag gacgagctgg tggcggagat gggcccactc cccagaaccc
     1621 acgggatgct cgatgccatg ctccacgacc cggagccggt gcggctggct gacatcacca
     1681 aggaccccag attccggggc tggtggccgc gggcccatcc cttgatgcgc tcgtttctcg
     1741 gagtgccgat cgtcgcccag gacgaggtga tcggagcgtt ctatctgacg gacaagatcg
     1801 gggccgccgt gttcaccgca gccgaccagg ccaacatcgg gctcct
//
LOCUS       DATITW010000256         2402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_372863, whole genome shotgun sequence.
ACCESSION   DATITW010000256 DATITW010000000
VERSION     DATITW010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372863"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..907
                     /locus_tag="VLS86_03605"
     CDS             335..907
                     /locus_tag="VLS86_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016639691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49609.1"
                     /translation="MLMTIETLRQIESGRIDSVYRRWAAARVKPGSRQRTMVGVIEIV
                     SVTETDPELLSAEDATAAGFDSVADLVLAAGDRGPILYRIGVRHVGPDPRWALREVVP
                     DAAELTDIRNRLDRLDAGSPHGPWTRETLALIASSPGVRAEDLARSVGREKMPFKLDV
                     RKLKELGLTESLETGYRLSARGRAVHEAMG"
     gene            complement(904..1887)
                     /locus_tag="VLS86_03610"
     CDS             complement(904..1887)
                     /locus_tag="VLS86_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49610.1"
                     /translation="MFSTKFSDRDVDLLLSGETPQNDDLAELAEFVGIIRAQGAPVPS
                     EAAVARFAAKAAQYARQSPSPITGGQVPARRPALARLRPQVALTLVALFMLSGVAVAA
                     DAAVPGSTLYPIDRALENVGIGNGRSAERLEEAGALIADGKVEAALAHATEALDESDL
                     DDPAGIEQAKAAVIAASERLSSSPDAGGVPGAELRNVSALLSYLEANLGEEEGRGGRE
                     FGQGVADLARLISMEKRGESPVDDPGPAEDETADEVGGRPAVSPADDGRPPADPPGQS
                     DGDREQTGNGNGASGNGDQGNDNGSRDNGSRGNGPPDESPSVTAPGRPDRP"
     gene            complement(1914..>2402)
                     /locus_tag="VLS86_03615"
     CDS             complement(1914..>2402)
                     /locus_tag="VLS86_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSO49611.1"
                     /translation="ANLYRRLAGPVTGYLGSRGATDREDVAAETFLQVARNIAGFEGG
                     EAAFRSWVFVIAHRRLIDMRRAKQRRPRIDTLPDDERVLGGGDVEEEAIGNLMAAELV
                     TALDQLTGDQRDVLHLRIIGDLSVEETALVIGKREGAVKALQRRALHALREHLAENRV
                     SL"
BASE COUNT          373 a          795 c          808 g          426 t
ORIGIN      
        1 gcaagcggtg gagagaggga cgatggtgaa cagaaatccc tgcaactcgc ggatccaatc
       61 ggtgcgggcc tcgagtatgg gattgagcca ctggtagagc aggtaggcgc cggagaacgc
      121 cagtccggca cccagcaccc cacccagtgc tcggagtgcc atctgtcgtc gaccttgtga
      181 ggtggcggtg tcgggcttca gcatcgaatc tcctacggca agagtgccga ccgggcgccc
      241 accgatgcca ggggccaaat gccctgtctc ggcgacgaag gacgcttttc ctgacttttg
      301 gggacaccac catctccctt aacctccgcc gccgatgttg atgaccatcg agacgctccg
      361 tcagatcgaa tcgggacgca tcgacagcgt gtaccggagg tgggcggccg ccagggtgaa
      421 acccggatca cgacagcgca ccatggtggg agtgatcgag atcgtctcgg tcaccgaaac
      481 cgatcccgag ctgttgagcg ccgaggacgc gactgctgcc gggttcgact cggtggcgga
      541 cctcgtcctg gctgcaggtg accgggggcc gatcctgtac cggatcgggg ttcgccacgt
      601 cggccccgat cctcgatggg cgctgcgtga ggtcgtgcct gatgcggcgg agttgaccga
      661 catccggaac cggctcgacc gcctcgacgc cgggagcccg catggtccgt ggaccaggga
      721 gaccctggcc ttgatcgcct cgagcccggg ggtgcgtgcc gaggatctcg cccggtcggt
      781 ggggcgggag aagatgccgt tcaagctcga tgtgcgaaag ctcaaggaac tggggttgac
      841 cgagagcctg gaaaccggct accggctatc cgctcggggg cgagccgttc acgaagcgat
      901 gggctagggc ctgtccggcc ggcccggggc ggtgaccgat ggcgattcgt cagggggacc
      961 gttgcctcgg ctcccgttgt cgcggctccc gttgtcgttc ccttgatctc cgttgccgct
     1021 tgctccgttc ccgttcccgg tctgctcccg atcgccgtcc gactggcccg gtgggtcggc
     1081 gggcggccga ccgtcgtcgg cgggtgacac cgccggccga cctccgacct cgtcggcggt
     1141 ctcgtcctcg gccggcccgg ggtcgtcgac ggggctctct ccacgcttct ccatggagat
     1201 gagccgggcc agatcagcga caccctggcc aaactcacga ccaccccggc cctcttcctc
     1261 acccagattc gcctctagat agctgagcag ggcagaaacg ttcctcagct cggctccggg
     1321 gactcctccg gcatcggggc tgctcgacag tcgctctgag gcggcgatca ccgcagcctt
     1381 cgcctgctcg atcccggccg ggtcgtcgag gtcggattcg tccaatgcct ccgtggcgtg
     1441 ggccagagca gcttccacct ttccatcggc gatcagggcc ccggcctcct ccaacctctc
     1501 tgccgagcgt ccgttgccga tcccgacgtt ctccagcgcc cggtcgatcg ggtacagggt
     1561 gcttcccggg accgcggcgt cggccgccac tgcaacaccg gacagcatga agagtgccac
     1621 caaggtgagc gcgacctggg gccgcaagcg ggcgagggcg gggcgtctgg cgggcacctg
     1681 cccaccggtg ataggggatg gagactggcg tgcatattgc gcggccttcg cggcgaaacg
     1741 ggccaccgcg gcctcagacg gcaccggcgc tccctgggcg cggatgattc cgacgaattc
     1801 ggccaactca gccagatcgt cgttctgagg agtctccccg gagagcagga ggtcaacgtc
     1861 tcggtcggag aacttcgttg agaacatctc ggttctgtaa tcgcccgaat gtctcacagg
     1921 gatactcgat tctcggcgag atgctcccgg agcgcatgta gggcccgtcg ttgcagggct
     1981 ttcactgcgc cctctcgctt cccgatgacg agagcggtct cttccaccga gagatcgccg
     2041 atgatcctca ggtggagaac gtcacgttgg tccccggtga gctggtccag tgccgtgacc
     2101 agctcggccg ccatcaggtt gccgattgcc tcttcttcga cgtccccacc acccaggacc
     2161 cgctcgtcgt cggggagggt gtcgattcgt ggtcggcgct gcttggcgcg gcgcatgtcg
     2221 atgaggcggc ggtgggctat cacgaacacc caggagcgga acgcggcctc gcctccctcg
     2281 aatccggcga tgttgcgggc gacctggagg aatgtctctg cggcgacatc ttcacgatcg
     2341 gtcgcccctc gtgagccgag atatccggtg accggcccgg cgagtctccg gtacagattg
     2401 gc
//
LOCUS       DATITW010000257         1513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_12888, whole genome shotgun sequence.
ACCESSION   DATITW010000257 DATITW010000000
VERSION     DATITW010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12888"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="VLS86_03620"
     CDS             <1..1258
                     /locus_tag="VLS86_03620"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF010372.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HSO49612.1"
                     /translation="GPVDLLSMLVGGEAVNASDVHYSHPSNLLRPTEPAGMWDGWETK
                     RRRGPGNDWVEMKLGMPGTIETVVVDNPHRFGYVRLNIYPDGGVARLRVEGTPIPSRS
                     QVCPELEPVDLLSMLVGGEAVNASDVHYSPPSNLLRPTEPVGMWDGWETKRRRGAGND
                     WVELKLGMPGTIKTVVVDTRHFKGNAPGWVSILVSEDGVDWDTVLDRVPVSPHDVARL
                     ELPVPSHAAFLRVDIHPDGGLARVRALGRPDRDVVIQKRMTYLNSLTDSAARRFFKTA
                     CVTGPWDDAMARGRPYSGLEAVLDAAATAFDLLGAADWMEAFSGHPRIGEKGDQTANR
                     EQSGTEGAATSTLAAIAEVNRQYEERFGFTYIVYASGKSAEEMLAIAESRTGNTREQE
                     IDTASAEQRAITATRLRRMLCEGDQP"
     gene            1255..>1513
                     /locus_tag="VLS86_03625"
     CDS             1255..>1513
                     /locus_tag="VLS86_03625"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF012785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HSO49613.1"
                     /translation="MTSVSTHVLDTSTGEPVVGIRVTLERSVDGGWQHLSEGETDVNG
                     RIMMLAGAVLTGVHRLVFATGDAGNVFYPQVHIVVSLDEEQD"
BASE COUNT          280 a          459 c          517 g          257 t
ORIGIN      
        1 gggcccggtc gacctgctct cgatgctggt cggcggggag gcggtgaatg cctccgatgt
       61 ccactactcg catccatcca acctgctccg cccgaccgag ccggcgggga tgtgggatgg
      121 ctgggagacc aagcggcgaa ggggccccgg caacgactgg gtcgagatga aactggggat
      181 gccgggcacc atcgagaccg tcgtggtcga caacccgcat cgtttcggat acgtcagact
      241 caacatctat cccgatgggg gagtggcccg gctccgggtg gagggaaccc cgatcccgtc
      301 acgatcccag gtatgccccg aactggaacc ggtcgacctg ctctcgatgc tggtcggcgg
      361 ggaagcggtg aatgcctccg atgtccatta ctcccctcct tccaacctgc tccgccctac
      421 cgagccggtg gggatgtggg atgggtggga gaccaagcgg cgaaggggcg ccggcaacga
      481 ctgggtcgag ttgaagctgg ggatgccggg caccatcaag accgtcgtcg tcgacacccg
      541 tcatttcaag ggcaacgcgc cggggtgggt ctcgatcctc gtatccgagg acggggttga
      601 ctgggacaca gtgctggacc gggtcccggt cagcccacat gatgtcgcgc gtctcgagtt
      661 gccggtcccg agtcacgccg cgtttctgcg ggtcgacatc catcctgacg gtggtctggc
      721 acgggtcagg gcactaggtc gccccgatcg cgatgtcgtg attcagaagc ggatgaccta
      781 cctgaactcg ttgaccgact ctgcggcacg acgcttcttc aagacggcgt gtgtcacggg
      841 accctgggat gacgcgatgg cccggggacg cccctactcc ggtctggaag cggtgctcga
      901 tgcagccgct actgccttcg atctcttggg tgcggcggat tggatggagg ccttctccgg
      961 acacccccga atcggggaaa agggcgacca aaccgccaat cgggaacagt ccgggacgga
     1021 gggagcggcg acgtcgaccc tggccgcgat cgccgaggtc aaccgccagt acgaggagcg
     1081 attcgggttc acctatatcg tctacgcctc ggggaagagc gcggaggaga tgctggcgat
     1141 cgcagagtcc aggacgggta acacccggga gcaggagatc gacactgcgt cggccgaaca
     1201 aagggcaatc acggccacca gacttcggag aatgctgtgt gaaggagacc agccatgaca
     1261 tccgtcagca cccatgtcct cgacacctcc accggcgaac cagtcgtcgg gatccgtgtg
     1321 accctggagc ggtcggtcga cgggggatgg cagcacctgt ccgagggtga gaccgatgtc
     1381 aatggccgaa tcatgatgtt ggccggggcg gtcctcaccg gtgtgcaccg gctggtgttc
     1441 gccaccggag acgcgggtaa tgtcttctat ccccaggtcc acatcgtggt ctcattggac
     1501 gaagagcagg atc
//
LOCUS       DATITW010000258         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_613789, whole genome shotgun sequence.
ACCESSION   DATITW010000258 DATITW010000000
VERSION     DATITW010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613789"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VLS86_03630"
     CDS             <1..198
                     /locus_tag="VLS86_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49614.1"
                     /translation="AAVFGDQTYARSFSPGQVAMAWETLAGGMALVGDLPPDMHEGVL
                     AGLPAPEDPGLFRRLWRNLFG"
     gene            complement(229..1299)
                     /locus_tag="VLS86_03635"
     CDS             complement(229..1299)
                     /locus_tag="VLS86_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HSO49615.1"
                     /translation="MSDDIFSKLFELFDQPGPINWKLAAEVARHLSGEPEPVEPWAAE
                     EFRELARLAEYRIEQIAPFRVIPASDVIAVDARGWTDRNLETFGSLVEPFASSVAADG
                     PAAPLMAQLSPAIIGLQVGSLVGALSTWVVASFDAGLPQEHPGPLTVIVPNIIELTRS
                     SGQDEREVRLWVVANEVAFRAVSQLPWIADHLHQLASAYAGAVKIDPTLLSGLMAGGS
                     DPVAIEQAIDEAGGLEGIIGGEEAVAPRLELEAFLGAITGCARLLARRAIGELSTEFD
                     QISAHRDRAREEAQTAPTIGMGPVPAEATQLGDAFNQEVERRYGDDALETLWADPSRM
                     PTASELRDPTAWAARVLLDGWS"
     gene            complement(1316..2497)
                     /locus_tag="VLS86_03640"
     CDS             complement(1316..2497)
                     /locus_tag="VLS86_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSO49616.1"
                     /translation="MTDTGPSGPQLWEPPSETPVGYEDTPASPPPTAKTKIDAWTIVI
                     GLLGGVLLGTGVTLAVLGFTGVFEEPTPPTIPPPPTITLPPPTTAPAVVVEVGNATEV
                     AQRAIPSSVAVETGSALGDGGGSGVVYSSDGYIITNHHVVDGATEVVVVFADGGRWEA
                     EVIGSDPLTDIAVLRIGRGDLTPIDIGSSAGLTIGERAIAVGNPLALEGGPSVTSGIV
                     SALDRSLTVETGTELYGLIQTDAPITRGSSGGALLDISARLIGITTAIAVSDVGAEGL
                     GFAIPIDMAIGVANDLIESGEVTHGVLGIQGETFHVEQGGAEYPVGVLVTDVSTGSAY
                     ELGGGQVNDVITAIDGVPITTMEALLTEMRTKRSGDEVTVAVSRSDSDVDLTISLGTL
                     E"
BASE COUNT          397 a          850 c          879 g          431 t
ORIGIN      
        1 gcggcggtct tcggcgacca gacctatgcg cgctcgttca gccccggtca ggtcgccatg
       61 gcatgggaga cactcgccgg cggcatggcc ctggtcggcg acctgccacc cgatatgcac
      121 gaaggtgtgc tggcgggact ccccgcgccg gaggacccgg gtttgttccg gcgtctgtgg
      181 cggaacctgt tcggctagga agccggctgg tttgcgtccg cgaggacgtc agctccaacc
      241 gtcgagcagg acccgggccg cccaggcagt gggatccctc agctctgagg cggtcggcat
      301 ccggctggga tcggcccaca gggtctccag ggcgtcgtca ccgtatcggc gctcgacttc
      361 ctggttgaag gcatcgccga gctgtgtcgc ctcggcaggc accgggccca tgccgatggt
      421 cggagcggtc tgagcttcct ctcgtgcccg gtcccgatgc gcggagatct gatcgaattc
      481 ggtcgagagc tcaccgatcg cacgtcgtgc gagaagccgg gcacatccgg tgatggcacc
      541 caggaaggcc tccaattcga gacgcggggc cacggcctcc tcgccaccga tgatgccctc
      601 cagcccgccc gcctcgtcga ttgcctgctc gatggcaacc gggtcgctgc cacctgccat
      661 cagtccgctg agaagtgtgg ggtcgatctt gaccgccccc gcataggcgg aggccagctg
      721 atggaggtgg tcggcgatcc acgggagttg ggagacggct ctgaacgcca cctcgttggc
      781 gaccacccac agcctcacct ctcgctcgtc ctgaccgctg gaccgggtca gctcgatgat
      841 gttgggcacg atcaccgtca gcggccccgg gtgctcctga ggcagcccgg cgtcgaagga
      901 ggccacaacc caggtggaga gggcgccgac cagggacccg acctgaagcc cgatgatcgc
      961 gggggagagt tgggccatca gtggggcggc cgggccgtca gcggcgactg atgaggcaaa
     1021 aggctcgacc agtgatccaa aggtctccaa gttgcggtcg gtccatcccc gggcgtcgac
     1081 tgcgatcaca tcactcgccg ggatcacccg aaacggggcg atctgctcga ttcggtactc
     1141 ggcgagacgg gccagctccc ggaactcttc ggcggcccac ggctcgaccg gctcgggctc
     1201 gccggagagg tgacgggcca cctcggctgc cagcttccag ttgatcggtc ccggctggtc
     1261 gaacagctcg aacagcttgg agaagatgtc gtcgctcatt gcctgctgcc tgccatcact
     1321 cgagcgtccc cagactgatc gtgagatcca cgtcggaatc ggatcgagac actgccaccg
     1381 tcacctcgtc gcctgacctc ttggttcgca tctcggtgag caaggcctcc atggtggtta
     1441 tcgggacacc gtcgatggcg gttatcacat cgttgacctg cccacccccc agctcgtagg
     1501 ccgagccggt gctgacgtcg gtgaccagca cccccaccgg gtactcggcg ccgccctgtt
     1561 cgacatggaa ggtctctccc tggatgccga ggacgccatg ggtgacctcg cccgactcga
     1621 tcaggtcgtt ggcaacaccg atcgccatgt cgatcgggat cgcaaagcca agtccttcgg
     1681 ccccgacgtc ggagaccgca atcgcagtcg tgatcccgat gaggcgggcc gagatgtcga
     1741 ggagggcgcc gccggaggac cctcgtgtga tcggggcgtc ggtctggatc aagccgtaga
     1801 gctcggtccc ggtctcgacg gtcaacgagc ggtcgagggc gctgacgatc ccggaggtga
     1861 ccgagggccc tccctccagg gccagcgggt tgccgaccgc gatcgcccgc tcgccgatgg
     1921 tcaaaccggc ggacgatccg atatcgatcg gggtcaggtc tccgcggccg atccgcaaca
     1981 ccgcgatgtc ggtgagcggg tcactgccga tcacctccgc ttcccatctc cccccatcgg
     2041 cgaagaccac gacgacctcg gtggcgccgt cgacgacgtg gtgattggtg atgatgtatc
     2101 cgtcggagct gtagacgacg ccgctgccgc ctccatcgcc gagggcgctc ccggtctcga
     2161 cggcgacgct cgacgggatc gcccgttgtg ccacctcggt ggcgttgccg acctcgacga
     2221 cgacggcggg ggcggtggtc ggtgggggca gggtgatggt cggcggcggc gggatggtgg
     2281 gcggcgttgg ctcctcgaag accccggtga atccgagaac ggccagggtg accccggtgc
     2341 ccagcagcac cccacccagg agaccgatca cgatcgtcca ggcgtcgatc ttggtctttg
     2401 cggtcggcgg cggggacgcg ggcgtgtcct cgtacccgac cggggtctcg ctggggggct
     2461 cccaaagttg cggcccgctg gggccggtgt ccgtcatagg ctttggattc tatggatgct
     2521 tccagccaac tcgtcagagt gtcggtcggg gagggac
//
LOCUS       DATITW010000259         6359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_172943, whole genome shotgun sequence.
ACCESSION   DATITW010000259 DATITW010000000
VERSION     DATITW010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172943"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..370
                     /locus_tag="VLS86_03645"
     CDS             158..370
                     /locus_tag="VLS86_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49617.1"
                     /translation="MTPEELTRLLEELQAEIDAASPVATLQDDVASSDHGVAALRLRE
                     VEPIVEIDRLSDDPLTLEVSGLRHRR"
     gene            complement(387..1853)
                     /locus_tag="VLS86_03650"
                     /pseudo
     CDS             complement(387..1853)
                     /locus_tag="VLS86_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            1931..2428
                     /locus_tag="VLS86_03655"
     CDS             1931..2428
                     /locus_tag="VLS86_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HSO49618.1"
                     /translation="MVLEAERDIRNRLGDRPLDFEALLAVSNIYRAANAVRNRMERDV
                     LSPAGLTWGGFTILFVLWVWGDSETGQLAEDCGLAKGTLSGMLTTLEKSQLVGRSRHA
                     EDGRRVLVTLLDKGRETIETVFPEFNRHEAKFTSDLDDEERRELARLLRRVTATADGV
                     PLVAE"
     gene            2540..3442
                     /locus_tag="VLS86_03660"
     CDS             2540..3442
                     /locus_tag="VLS86_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HSO49619.1"
                     /translation="MLTSRREQVMGEIVGAGLISHAPTIMFDEATRLEINDGKEISLV
                     PGLVRLRDQVFDRLQPDTIVIVDSHWFSTVEHLLAAQERRQGIFTSSELPRGMAQIPY
                     DFPGDPELAKLAATLGDELGTPTHASEDDYLPVYYATLNLLHYLHKEEKVVSVSVVQT
                     GETDDFMTMGEAIGEAVARLDRRAVILASGGMSHTFWPLKQIAKHEASDPVHIITPEA
                     RAADEERLEWMVAGDHARVVDTMPDYFRQSPEGAFGHYLMMLGAIGGRDCIARGELFS
                     NYENATGTGQVHVWFERPEGGWTG"
     gene            3480..4994
                     /locus_tag="VLS86_03665"
     CDS             3480..4994
                     /locus_tag="VLS86_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HSO49620.1"
                     /translation="MVTESRPHDVVIDLVRLATSLEALSIPATTDRDRLGARRDWILR
                     TIRGYLIPRIEDPTGPMVVVCAGPTGAGKSTLLNSVAGGDHSIAGPLRPTTKAPLVLA
                     SRDHAASYSTIGGVGCRVVTGRAPILGELTLVDTPDIDSTSLEHRAIAETMIDNADVV
                     VYVTSASRYADLVPWEVLRRAHSRGVPVVPVLNRLKSSTSGALADYKTRLQAEGLTSE
                     VVAVHEHHLAKGAQSVPLAVIQELRDRLVDVVSARQAGTTDAVRSVLETTLDEVGDVI
                     GRVNEMVTDTIDATDRARGELLVDLDRLLTRLRTNAGAHLDLAPLASLASRWFRIGWL
                     VRRNLPPAAAIAANHRLLDASLVATVDADIRRQVGGGSPIQREQASQLLGDTHSAVKA
                     AIATWHQDLDDFPEVIGAIDRSLASLLLARCAVEGHDPDMDAVFDLLTGSDDVAVSVA
                     RARERLEVHRLPVYSGVEYQVTSQLAMGVATDDALYRARVSLSAVIARSSFAHA"
     gene            4987..6225
                     /locus_tag="VLS86_03670"
     CDS             4987..6225
                     /locus_tag="VLS86_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HSO49621.1"
                     /translation="MRDALVGLLDKVDIAVAESRGVLAAADIAVVAQVASDTRLRLVY
                     PDSTVVVALAGGTGSGKSSLLNAIAGDEVALTGGIRPTTVAPLALVPRSQRAGLNGYL
                     DILGIEERVDHDGPQWLCLIDLPDNDSVEVEHRHQVAALIPRVDMVIWVTDPEKYRDA
                     TLHQGSIAPLSDYQAQFVFALNQIDRIDEQDNHLVLSDFAEALGEDGINDPFVITTAA
                     NPVAGPPIGIDRLLQQVERGRSQRETVHRKLLTDLANAASLLVASSSGAHGVDFERRW
                     AEELETAAGLGLAGEVTDAAQGLAWFVARLAEEVDGETTDRLREVSEEVPTRFIECLA
                     EVGTPAGPQDEQRWFWRSRVRRREPAAVDDPEAARALQSALDLAIGNPIRDLLAARGR
                     AHAAIADLALAVGHLERRGT"
BASE COUNT         1058 a         2141 c         2110 g         1050 t
ORIGIN      
        1 gcgagtacga caagcaactg gatggttatg cgtcccgtcg ctcaggcgag accgcagtgg
       61 tgatcctgga accggtgtcc accgacgacc actgactctt ttcgcgtcct cgcccattgc
      121 catgcttacg aacaaatgtt cgcctatgct gcgaagtatg acgcccgaag aactgactcg
      181 cctcctcgag gagttgcagg ccgagatcga tgcggcgtca ccggtcgcca cgctccagga
      241 cgacgtcgcc agctctgatc acggcgtggc cgcgttgcgt ctgcgcgaag tcgagcccat
      301 cgtggagatc gatcggctct ccgatgaccc gctcaccttg gaagtctccg ggcttcgtca
      361 ccggcgctga gagccccgcc gccgactcac ccaaccgtgc cctggctgat ctgcaccgtc
      421 ttcaggtcgg agtggaagtc cagggcgtaa tccccaccct cacgtccgat cccggagatc
      481 ccgatcccgc caaacggtgc ggtgaggtcc ctgaccagga acgtgttcac ccagatcgtc
      541 ccggctcgta tcccgcggcc gacccgctcg gcccgctcct cgctcccggt gtagacgatc
      601 cccgacaggc cgtagcgcgt cgagttggcg agatcgatcg cttcgcgctc gtcggcaaag
      661 gtctggtagg tgagcaccgg tccgaacacc tcacgctgca cgatctccga gtcgttggcc
      721 acgggctcga tcagcgtcgg caggtaccac aggccatcct gtctccatcg ctcgccgcca
      781 cgcaggacgc gatcgccgtg tttccgcgcc ctctccacga atccctcgac cctggccagg
      841 tgatcggggt ggatcagggg gctgatcgtg gtccggggat cgcggctgtc gccgatcacg
      901 tgggcgtcgg tgtaggtgtt gaacaactcg aggaactccg ctgagatccc ctcctcgacg
      961 atgagtcggg tcccggcgag gcatacctgt cccgagtcgt cgtactgacc tgcagctttg
     1021 cgggcggcgg cgtccaggtc ggagtcggcg aagacgacca gcgggccctt gcctccgagc
     1081 tcggcggtga acgggacgat gtttcgggcg gcggagaccc cgatgtggcg ggcggtctcc
     1141 ggggagccgg tgaatgagag ccggcgcacc cttcggtccg agaccagggc ggcgcccacc
     1201 tcctcgccga tcccctggac gacgttgagc acgcccccgg gaagaccggc ctccatcgcc
     1261 aggtcggcga ggatcgacgc cgacaagggc gcccattccg ccggcttgag gatcgcggtg
     1321 ttgccggcgg ccagcgccgg ggccagcttc caggtcgaga gcagccaggg cgcgttccac
     1381 ggggtgatga tcacggccgg gccggccggc atgcgttgca cctagttgtc ggttcccttg
     1441 gacgaccacc ttcttccctc gaaggcgacg gcaagctcgg cgtagttccg aaaattctcc
     1501 gcgccccggt ggatgaccct ggcctggagg ctctccagca gcatggccat gtcgacgcac
     1561 tcgactccag caatcatctc gatgttctcg tcgatcaggt ctgcgagacg gtggagcggc
     1621 tctgcccgct gggccgggct gcgaccggcc cactcgggga aggcgtcgac cgcggctgtc
     1681 accgcctcgt ccgcggtgag ggcgtcgccc ttagccaccc gggccaggag ccagtcccat
     1741 tccagagggg agcgcacctc gaaggtgtcg ggggaggaga cacgtcggcc cccgatgaag
     1801 tggtcagggg acacggcggc gtcgagtagc tcgactctgg ttgaaggcat catgggatcg
     1861 ttagcaagct aaaggataag ctccactacc tccagacatg cccacaccac tcagcgatca
     1921 cagtgaccga gtggtcctcg aggccgagcg cgacatacgc aaccggctcg gggatcgacc
     1981 cctcgacttc gaggcgttgc tcgcggtgtc caacatctac cgcgcggcca acgcggtccg
     2041 caatcggatg gagcgagacg tgttgagccc ggccgggctc acctggggag ggttcaccat
     2101 cctgttcgtg ctttgggtgt ggggcgacag cgagaccggg cagttggccg aagactgcgg
     2161 tctggccaaa ggcaccctct cgggaatgct caccaccctg gagaagagcc agttggtggg
     2221 gcgctcccgt cacgccgagg acggaagacg ggtcctggtc acgctgctcg acaaggggcg
     2281 ggagaccatc gagacagtgt tcccggagtt caaccgccac gaggccaagt tcacctccga
     2341 tctggacgac gaggagagac gggagctggc ccgcctcctg cgccgggtca ctgccaccgc
     2401 cgacggagtg cccctcgtcg ccgagtgagg tggcggccac ccgagcacag cacccgtgtt
     2461 caccagcgga ctagcccctg acggtgagtt ggggccagcc atcgcagagg gagccgttac
     2521 ccgatagtct gtcgttcggt tgctaacgag ccgaagggag caggtgatgg gcgagatcgt
     2581 cggcgcaggg ctcatcagcc acgccccaac catcatgttc gacgaggcga cccggctcga
     2641 gatcaacgac gggaaagaga tcagtctggt ccccggtctg gtacgactca gagaccaggt
     2701 cttcgaccga ctccaacccg acaccatcgt catcgtcgac tctcactggt tttccaccgt
     2761 ggagcacctg ctcgccgccc aagagcgacg gcagggcata ttcacctcgt cggaactgcc
     2821 ccgggggatg gcgcagatcc catacgactt cccgggcgac ccggagttgg ccaagctggc
     2881 cgccaccctc ggggacgagt tggggacccc gactcacgca tccgaggatg actacctgcc
     2941 ggtctactac gcgaccctca acctcctcca ctacctgcac aaggaggaga aggtggtgtc
     3001 ggtctcggtg gtccagaccg gggagaccga cgatttcatg accatggggg aggcgatagg
     3061 cgaggccgtg gcgcgtctcg accgcagggc cgtgatcctg gcctccggag ggatgagcca
     3121 cacgttctgg ccgctgaagc agatcgcgaa gcacgaggct tcggacccgg tgcacatcat
     3181 cacccccgag gcgcgcgccg ccgacgaaga gcgtctcgag tggatggtgg ccggggacca
     3241 cgccagggtc gtggacacca tgccggacta cttccggcaa tcccccgagg gcgcctttgg
     3301 ccactacctg atgatgctgg gcgcgatcgg gggccgggac tgcatcgcgc gtggcgagtt
     3361 gttctccaac tacgagaacg caaccggcac cggacaggtc cacgtgtggt tcgaacgacc
     3421 cgaaggcggc tggactggct gacgcgaatt ccatgcgaag gcgtggcaca atctcgacca
     3481 tggtgaccga gtcgaggcct cacgatgtgg tgatcgatct cgtccgtctc gccacctcgc
     3541 tggaggcgtt gagcatcccg gcgacgaccg accgggaccg gcttggggcg agacgggatt
     3601 ggatcctgcg cacgattcgc ggctatctca tcccgaggat cgaggacccg acgggcccga
     3661 tggtcgtggt ctgtgccggg ccgaccgggg ccgggaagtc gacactgttg aactccgtgg
     3721 ccgggggtga ccacagcatc gccggcccac tgcgcccgac caccaaggcg cctctcgttc
     3781 tcgcctcccg cgaccatgcc gcgagctaca gcaccatcgg cggggtcggg tgcagggtgg
     3841 tcaccgggcg agccccgatc ctgggggagc tcacgctcgt cgacaccccc gacatcgact
     3901 ccacctccct cgagcacagg gcgatcgcag agaccatgat cgacaacgcc gacgtcgtgg
     3961 tctacgtgac atccgcttcc cgttacgccg acctggtgcc ctgggaggtc ctgcgcaggg
     4021 cccactcgag aggcgtgccg gtggttccgg tgctgaatcg gctcaagagc tcgacatcgg
     4081 gggccctcgc cgattacaag acccggctcc aggccgaggg cctgacctcc gaggtggtcg
     4141 cggtccatga gcaccacctg gcgaaggggg cgcagtctgt tccgctggcc gtgatccagg
     4201 agctgcgaga tcggttggtc gatgtcgtct cggcgcgaca agcggggacc accgacgcgg
     4261 tgcgctcggt cctggagaca accctcgacg aggtgggtga tgtgatcggc cgggtcaacg
     4321 agatggtgac ggacaccatc gacgccaccg accgcgcccg gggcgagctg ctggtcgacc
     4381 tcgacaggct cctcacccgg cttcgcacca acgccggggc gcatctcgat ctcgcgcctc
     4441 tggcctcgct cgcctcgagg tggttccgca tcgggtggtt ggtgaggcgg aatctcccgc
     4501 cggccgcggc gatagcggcc aaccatcgtc tcctcgacgc atcgctggtc gcaaccgtcg
     4561 acgccgacat ccggaggcag gtgggtggcg gcagccccat ccagcgcgag caggcgagcc
     4621 aactcctagg cgacacccac tccgcagtca aggccgccat cgccacctgg caccaggacc
     4681 tcgacgactt tccggaggtg attggcgcca ttgaccgcag tctcgcctcg ctgctactcg
     4741 cccggtgcgc cgtcgagggc cacgaccccg acatggacgc ggtcttcgac ctgctcaccg
     4801 gctccgacga cgtcgccgtc tcggtggccc gggcccggga gcgtctcgag gtccatcggt
     4861 tgccggtcta ctccggcgtc gagtaccagg tgacgtcaca gctcgcgatg ggagtggcca
     4921 ccgacgacgc cctgtatcgg gcgcgggtgt cgctttcggc tgtgatcgcg aggtcctcgt
     4981 tcgcacatgc gtgacgcgct tgtcggtctt ctcgacaagg tcgacattgc cgtagccgaa
     5041 tcgaggggtg tcctcgccgc agcagatatc gcggtggtcg cccaggtggc gagcgacacc
     5101 cgtcttcgtc tcgtctaccc ggactcgacc gtcgtggtgg ccctcgccgg ggggaccggc
     5161 tcgggcaaat cgagtctcct caatgccatc gccggtgacg aggtcgctct caccgggggc
     5221 atccgcccga cgaccgtggc cccgctggcc ctcgtgccac gatcacagcg agcgggtctc
     5281 aatgggtacc tcgacatcct cggcatcgaa gagcgcgtgg atcacgacgg gccgcagtgg
     5341 ctgtgcctga tcgatcttcc cgacaacgac agcgtcgagg tcgagcaccg gcatcaggtt
     5401 gcggcgctga ttccacgggt agacatggtc atctgggtca ccgatccgga gaagtaccgc
     5461 gacgccaccc tgcaccaggg ctcgatcgcc ccactctccg actaccaggc ccaattcgtc
     5521 ttcgccctga accagatcga ccgaatcgac gaacaggaca accatctcgt actgagcgac
     5581 ttcgccgagg cgctcggcga ggacgggatc aatgacccgt tcgtcatcac cacggccgcc
     5641 aacccggtgg ccggcccacc gatcggaatt gacaggctgc tgcagcaagt ggaacggggt
     5701 cggagccaga gggagacggt tcaccggaaa ctgctcaccg acctcgccaa tgcggcctcg
     5761 ttgctcgtcg cctcctcgtc gggtgcccac ggcgtggatt tcgagaggcg atgggccgag
     5821 gagctcgaga ccgcagccgg cctcggcctg gccggcgagg tcaccgacgc tgctcagggg
     5881 cttgcctggt tcgtcgcccg tcttgccgag gaggtggatg gggagaccac cgatcgactt
     5941 cgagaggtct ccgaggaggt tccgactcga ttcatcgagt gtctggccga ggtcggcacc
     6001 cccgcagggc ctcaggacga gcagcgctgg ttttggcgct cccgggtgcg gcggcgggag
     6061 ccggccgcag tggatgaccc tgaggccgcc cgagccctcc agtcggcgtt ggatctcgcg
     6121 atcgggaatc cgatccgtga tctcctcgcc gcccgtggcc gggcccatgc cgccatcgcc
     6181 gacctggcac tggcggtggg tcatttggag cgacggggca cgtaactacc ctcgcagtcc
     6241 gatggaagat gccgcggtca accgttgggt cgcctcggcg atcatcgtcg gagtgacgct
     6301 ggtgctctat gtcgtcctga tcagactcgg cgcccgtttc gtggatcgga tcggggccc
//
LOCUS       DATITW010000260         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_613847, whole genome shotgun sequence.
ACCESSION   DATITW010000260 DATITW010000000
VERSION     DATITW010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613847"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VLS86_03675"
     CDS             <1..261
                     /locus_tag="VLS86_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HSO49622.1"
                     /translation="RRRWMVGSREVSHLIDPRNMEPVRTPVLSATVVARSAVDAETGA
                     KAVLLNGEDGLTWAASADWIDLAIVVWHDGSVYATPGTVVAA"
     gene            258..818
                     /locus_tag="VLS86_03680"
     CDS             258..818
                     /locus_tag="VLS86_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49623.1"
                     /translation="MSLEWMLIRGSGVAAFALLSAATIWGLLVSSKLLGKLVKAKPLT
                     WFHESLGIGALIATVVHVVVLSIHDFLDFSWAEILVPGLSDWRPLAVALGVVSMYGLV
                     GVAGSFYVKRWIGQKLWRSIHFASFGVFVSSLVHGMWAGTDTGSPFMIGLYLGSALVV
                     FALLAHRLTATAPSQPQTPTRERIEV"
     gene            861..1121
                     /locus_tag="VLS86_03685"
     CDS             861..1121
                     /locus_tag="VLS86_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49624.1"
                     /translation="MINTPDLDQSRPIEEWSDGELLAEFRYLKAELSQEDPDYRDADG
                     APVDVIEQEMKRRGLTPDREDVIPDLSTPGREGNETTPRIRG"
     gene            complement(1204..1584)
                     /locus_tag="VLS86_03690"
     CDS             complement(1204..1584)
                     /locus_tag="VLS86_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49625.1"
                     /translation="MTDNTWHRWLAIAVSVGLIGLAGYLLVVGTAVNIDEIGGTPSSD
                     RESQVSAAIPLLAGVMYLSGLIWRRRTLVIGSAVVLTVYGALFLFSVGPYYAPIGIVM
                     WILGIVTSRHWDSRSNADVDIGAG"
     gene            complement(1593..>2401)
                     /locus_tag="VLS86_03695"
     CDS             complement(1593..>2401)
                     /locus_tag="VLS86_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49626.1"
                     /translation="VAGAELRSDPPAPIGSSQRSSAEFDAGDVALETVLAKGVKDGQT
                     CQFGNLEVSQNAGGLRTNWAVIRIDDSCEVSVAARWRGSLSDRPAAILPELEDSETHT
                     KSFTETVEVRRGDDRGKDTSQAVMAASSCSTHKQQIIHYGFGGPLDVLTKLIGTMVAC
                     ENATTFTSVSHTGSCVATNPPGGWTWVVERCYSGGVIATSTTAQTIEKGDFHCSPRAS
                     FPCNAVNPDGYDHTLRTVEQKARGGGALCFVSLTGLDVVASYKDIIQGCS"
BASE COUNT          441 a          758 c          737 g          465 t
ORIGIN      
        1 cgacgccggt ggatggtcgg gtcacgtgag gtgagccatc tcatcgaccc ccgcaacatg
       61 gagccggttc ggaccccggt tctcagtgca accgtggtcg ctcgctcggc ggtcgacgcc
      121 gaaaccggag ccaaggcggt gcttctgaac ggtgaggacg ggctgacatg ggccgcctcc
      181 gccgactgga tcgatctcgc catcgtcgtt tggcacgacg gatccgtcta tgcgactccg
      241 gggacggtgg tggcggcatg agcctcgagt ggatgctgat ccggggatcg ggtgtggccg
      301 ccttcgccct gctctctgca gcgaccatct gggggctgct ggtgagctcc aagttgctgg
      361 gaaagctcgt caaggccaag ccgctcacct ggtttcacga gtcgctggga atcggtgcct
      421 tgatcgccac cgtggttcac gtggtggtgc tgtcgattca cgacttcctc gacttcagct
      481 gggcagagat cctcgtcccc ggtctatcgg actggcggcc tctggcagtg gcgctcgggg
      541 tggtcagcat gtacgggctg gtgggcgtgg ccggcagctt ctacgtgaag cgctggatcg
      601 ggcagaagct gtggcgttcg atccacttcg cttcgttcgg ggtattcgtc tcgtcgctcg
      661 tccacgggat gtgggccggg accgacaccg ggagcccgtt catgatcggt ctctatctcg
      721 gatcggcgct ggtggtgttc gccctcctcg cccatcggct cacggccact gccccgtcgc
      781 aaccgcagac cccgacacgg gagcggatcg aggtctgaat ccgccagttt cgcccagttg
      841 cgtccagggt attggagggg atgatcaaca cacccgacct cgaccagagc cgcccgatcg
      901 aggaatggag cgacggggag ctcctcgccg agttccgata tctcaaagcc gagctgtcac
      961 aggaagatcc cgactatcgc gacgccgacg gcgctccggt cgacgtcatc gaacaggaga
     1021 tgaagcggcg cgggctcact cccgaccgcg aggatgtcat ccccgatctc tccaccccgg
     1081 gacgcgaggg caacgagacc accccccgaa tccggggctg acgagctgtc ggtgcgtcac
     1141 cgagggccgg gatccacagg ctcgtcgtag ttcagatgga cgacaccgtc cgcgaggtgg
     1201 cgcctatccc gccccgatgt cgacatcagc gttgctccgg ctatcccagt gtcgagatgt
     1261 cacgatcccc agtatccaca tcacgattcc gatcggggcg tagtaaggac ccacactgaa
     1321 gaggaagagg gcaccgtaga cggtgagcac cacggcagat ccaatgacca aggtccgacg
     1381 tcgccaaatc agcccggaga gatacatcac tcctgctagc agagggattg cggctgagac
     1441 ctgagattct cgatccgagg acggagtccc tccgatctcg tcgatgttca ccgcagtgcc
     1501 aacgactagg aggtagccgg ccagtccaat gagcccgacc gacactgcga ttgcaagcca
     1561 tcgatgccac gtgttgtccg tcatgtcgga acctaactgc agccctggat gatgtcttta
     1621 tagctggcca ccacatcgag ccccgtcagg ctgacgaagc acagtgcacc gccgccccga
     1681 gctttctgtt ccacggtcct aagcgtatga tcgtatccgt caggattcac cgcattacac
     1741 gggaaggaag cccgtggact gcagtggaaa tcacccttct cgatagtctg cgcagtggta
     1801 gatgtggcaa tgaccccacc gctgtagcac ctttccacga cccacgtcca ccctccaggt
     1861 ggattggtgg caacgcacga tccggtgtgc gagacagatg tgaacgtggt cgcgttctca
     1921 cacgccacca tggttccaat cagtttcgtc aggacgtcga gaggtccacc gaacccgtag
     1981 tggattatct gctgtttgtg cgtggaacag gaggacgctg ccatcaccgc ttgtgaggtg
     2041 tccttacctc gatcgtcacc tctgcgaacc tccaccgtct cggtgaagct cttcgtgtgg
     2101 gtctccgaat cctcgagctc gggcaggatt gcagcgggtc ggtcgctcaa ggatccgcgc
     2161 catcgtgcag caaccgagac ctcgcacgag tcatcgattc gaatgaccgc ccaattcgtt
     2221 cggaggcctc cagcattctg gctgacctcc aggttgccga attgacaggt ctggccgtcc
     2281 tttacacctt tggccaagac cgtttcgagg gctacatcgc cagcgtcgaa ttcagcggac
     2341 gaccgttgcg aactcccgat tggagccggt gggtctgacc ggagctccgc gccagccacc
     2401 a
//
LOCUS       DATITW010000261         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_653783, whole genome shotgun sequence.
ACCESSION   DATITW010000261 DATITW010000000
VERSION     DATITW010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653783"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VLS86_03700"
     CDS             <1..205
                     /locus_tag="VLS86_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HSO49627.1"
                     /translation="TELVIGPRHQMRVVDGLITGWHDPASTHLALLEALAGRSLLTDS
                     YQSALDTGYLWHEFGDSHLILPG"
     gene            complement(290..1504)
                     /locus_tag="VLS86_03705"
     CDS             complement(290..1504)
                     /locus_tag="VLS86_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSO49628.1"
                     /translation="MFDVAEAPQDVVAPEDLVEELASLFARRDRDDARIARLLAETGE
                     TGAFRRDGYPSLTTLLKHRMSLHPGEAHRLVTRGNGLAQAPLVALAYESGSLSGAQVD
                     VLLEARWTAPEAFSEAEGRLVTLALDTPLVRDLKKHLDYWLDQVAADDLGADRNLVRD
                     LRSLTLRRDGEMMRINGWVDIEAGERLRAELEPGPPAEGDNRSTPARRADVLLDILNG
                     ASDRPNLIVHVSAATLARREPGISETGNGTFLTADEIKRLACDANLTRVVFDPESQPL
                     DVGRAKRLVTPALRAAVCARDLRCVFPGCDRPSNWCDVHHLTHWADGGDTSVANLVLL
                     CRHHHVLIHEGGWTIKGMPGDLRVYRPDTSELGADPPPRHSVYVSDKPRAELPPGGLR
                     EIIRNLPRLTGS"
     gene            complement(1592..1834)
                     /locus_tag="VLS86_03710"
     CDS             complement(1592..1834)
                     /locus_tag="VLS86_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSO49629.1"
                     /translation="MTEVVHNRIAMLRAERGISRRQLSEALGVHYQTIGYLERGEYSP
                     SLFLALKIAEYFEVPTEVVFSTAPFARLGSKSVQAG"
     gene            complement(1831..2685)
                     /locus_tag="VLS86_03715"
     CDS             complement(1831..2685)
                     /locus_tag="VLS86_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49630.1"
                     /translation="MNHTRHAVRTGLNRGWTEFMLSLRSPQDQGFYLFMSAGVLLYLY
                     FNQNTEVEGTTLLYPTVALPSILGAMIAFGLIIGPAYSLAMEREDGTLLRAKAVPHGI
                     TGYVSGQVVLHSAGLVPSIALILIPGALLFEGLMSGGPGGWVTFVWVLILGLLATLPL
                     GIIIGSLVPGVQKVGTWGMLPILLLLGISGIFAPIQGLWGWVQVVAQMFPIYWLGLGM
                     RSAFLPDEAAVIEIGGSWRSVQTALVLGAWAVLGLVVAPIVLRRMARRQSGSQVEAAR
                     HEATQWVK"
     gene            complement(2682..>3031)
                     /locus_tag="VLS86_03720"
     CDS             complement(2682..>3031)
                     /locus_tag="VLS86_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO49631.1"
                     /translation="TTILLTTHDLDEAEKLADRILILAAGRIVADGSADQLARRVTRD
                     AQVRWSRDGEHFVHATPDATGFVRVLFAQYGDEIADLEVRRATLEDTYIAMVQQHETG
                     HGEKVTAFEEVKA"
BASE COUNT          548 a          975 c         1007 g          501 t
ORIGIN      
        1 gaccgagctg gtgatcggcc cccggcacca gatgcgtgtc gtcgacggcc tgatcaccgg
       61 gtggcacgac ccggcgtcca ctcatctcgc cttgctcgaa gcactggccg gccgaagtct
      121 gctgacggac agctaccaga gcgccctcga caccggatat ctatggcacg agttcggtga
      181 cagccatctg atcctccccg gttgagtcgc atggggcacc gagatcagcg ctggatgctg
      241 ggcgagggcc ggatcgagcc agccgagggt tggggaggct cgaggtcggc taggacccgg
      301 tgagccgggg aagattgcga atgatctcgc gcaagccacc cggtggcagt tcggctcgtg
      361 gcttgtcgct cacgtagacg ctgtgccggg gtggcgggtc ggctcccaac tcgctggtgt
      421 cgggtcggta gacccggagg tcgccgggca tccccttgat cgtccaacca ccttcatgaa
      481 tgagcacgtg gtgatgacgg cagagcagga ccaggttggc gacgctggtg tcgcccccgt
      541 ccgcccagtg tgtgagatgg tggacgtcgc accagttcga gggtcgatcg cagccgggga
      601 acacgcatct caagtcccgg gcgcaaaccg cggctcgcag ggccggggtg accagccgct
      661 tggcacggcc cacgtcgagt ggctgggatt cggggtcgaa gacgacccgg gtcaggttgg
      721 cgtcacaggc gagacgcttg atttcgtcgg cagtgagaaa cgtgccgttg ccggtctcgg
      781 agattcccgg ctcacgtcgg gcaagagtcg ccgcggagac gtggacgatg agattgggcc
      841 gatccgatgc gccgttgagg atgtcgagga ggacatcggc gcggcgggca ggagtggacc
      901 tgttgtcccc ttcggccgga ggccccggct cgagctcggc gcggagtctc tcgccggctt
      961 cgatatcgac ccaaccgttg atccgcatca tctccccgtc ccggcgcagg gtcagggaac
     1021 ggaggtcacg caccagattc cggtctgctc cgagatcgtc ggccgccacc tggtccagcc
     1081 agtagtcgag atgcttcttc aggtcccgaa ccaacggagt gtccagggcc agtgtgacca
     1141 gtcgcccttc ggcttcgctg aacgcctcgg gtgcggtcca tctcgcctcg agcaggacat
     1201 cgacctgggc cccgctcagc gagccggatt cgtaggccaa ggcgacgagc ggagcctgcg
     1261 ccaacccgtt gcccctggtc accaggcgat gggcctcgcc ggggtggagc gacatccgat
     1321 gcttgagcaa ggtggtcaat gaggggtagc cgtcacgacg gaaggcgccg gtctccccgg
     1381 tctcggcgag gaggcgggcg atccgggcgt catcccgatc gcgtcgggcg aacagtgacg
     1441 ccagctcctc gaccagatcc tctggtgcga ccacatcctg gggggcttcc gcgacatcga
     1501 acatatgttc gctacgctac cgcaggcctg ggacagaaac cacccctctg atttgatcaa
     1561 atcatgagag gttgaggacc tcgcgcgcgg ctcagccggc ctggaccgat ttgctcccga
     1621 gtcgggcgaa gggggcggtg gagaagacca cctcggtcgg cacctcaaag tactccgcga
     1681 tcttgagcgc gagaaacagg ctggggctgt actcgccgcg ctccaggtat ccgatcgtct
     1741 gatagtggac cccgagggcc tcactcagct ggcgacggga tattccccgc tccgcccgaa
     1801 gcatggcaat gcggttgtgg acgacctcgg tcatttgacc cactgcgtgg cttcgtgcct
     1861 cgcggcttcc acctgggagc ccgactggcg gcgggccatc cggcgcagga cgatcggggc
     1921 caccaccaga ccgagcacgg cccaggcgcc caacaccagc gcggtctgca ccgaccgcca
     1981 cgagccaccg atctcgatga ccgccgcctc gtcgggtagg aacgccgagc gcatcccgag
     2041 ccccagccag tagatgggga acatctgtgc cacgacctgc acccatcccc agagaccttg
     2101 gatcggggcg aagatcccgg agatcccgag cagcaacagg atcggcagca tgccccaggt
     2161 tcccaccttc tgcaccccgg gaaccagcga tccgatgatg atcccgaggg gaagagtcgc
     2221 cagcaacccg aggatcagga cccagacaaa ggtgacccag ccgccgggac caccactcat
     2281 cagcccttcg aagagaaggg cccccgggat caggatcaat gcgatcgaag gcaccaggcc
     2341 cgccgagtgc aacaccacct gacccgacac gtatccggtg atgccgtggg gaaccgcctt
     2401 ggcccggagc agggtgccgt cctcgcgctc catggcgagg gaataggcgg ggccgattat
     2461 caggccaaaa gcgatcatgg cgccgaggat gctgggcaga gccacagtcg ggtagagcag
     2521 cgtcgtccct tcaacctccg tgttctggtt gaagtagagg tagagcaaca cgccggcgga
     2581 catgaacagg tagaacccct ggtcctgagg gcttctcaga ctcagcatga actcggtcca
     2641 cccccgattc agaccggtac gaacggcatg tctggtgtgg ttcatgcttt cacctcctcg
     2701 aatgcagtga ccttctcgcc gtggccggtc tcgtgctgtt ggaccatggc gatgtaggtg
     2761 tcctccagag tcgcccgccg cacttcgaga tctgcgatct catcgccgta ttgagcgaac
     2821 agaacacgga cgaacccggt ggcgtcgggg gtggcatgga cgaaatgctc tccatcgcga
     2881 ctccaccgca cctgagcatc ccgggtcacg cgtctggcga gttgatctgc gctgccgtcg
     2941 gcgacgatgc ggcccgcggc caagatgagt atccgatcgg cgagcttctc cgcctcgtcg
     3001 agatcgtggg tggtgagcag gatcgtcgtc t
//
LOCUS       DATITW010000262         1951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_413656, whole genome shotgun sequence.
ACCESSION   DATITW010000262 DATITW010000000
VERSION     DATITW010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413656"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VLS86_03725"
     CDS             <1..553
                     /locus_tag="VLS86_03725"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSO49632.1"
                     /translation="LATQNPIEYEGTYPLPEAQLDRFMFKVKVNPPTADTLDEILRRT
                     TSGLITEVPRVVDAETVSSMLATTPTVPIAAHLIRYVSLIIEATHPDAESAPASVRRY
                     VRWGASPRGGQAMVLAAKATALLEGRPHVTAEDIRWAATSALRHRLVLGYEATAEGVR
                     SESIIGDVIKQVSEPKPDIRGVP"
     gene            550..1407
                     /locus_tag="VLS86_03730"
     CDS             550..1407
                     /locus_tag="VLS86_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSO49633.1"
                     /translation="MSLLSPSFMARLESLQLHSRHRLVGRFGGEHRSKRYGNTVDFAD
                     FREYHPGDDFRRIDYHVLARLDQVLIKLFEADDEVTVRILIDTSASMAVEGKLHQAKR
                     LAAALGFVALTAHDAVSIHTFPADGPPPRFAGRALVPDLFAYLESLQPKGLTPFAQAA
                     GHLLGRSGPAGITIVISDLLTPEWRSLMRLRASGSDLTVFHLLAEEDVAPEFAGDLML
                     VDREGGERLTVSVTDEVARSFRVRVAEWQNLVRSNSRGVGAVYMPVRPTQDIERLLLT
                     SWRDAGVLR"
     gene            1404..>1951
                     /locus_tag="VLS86_03735"
     CDS             1404..>1951
                     /locus_tag="VLS86_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSO49634.1"
                     /translation="MTLLAPIGLAALLLGAAVVILHMLTPRRPPTPVSSLLHWDGLKH
                     SITAAEPWQKLRWSLLLILQLLAVALFALALSQPARVEVADLAEHTVFIVDASGSMSA
                     IDGSPDRLAEAVQRAAELQEDIPQGGASSVVVASTNPAVLLQESTSPDEFQRAMASIR
                     ASSGDADYEAAFALAESLVSAE"
BASE COUNT          309 a          678 c          629 g          335 t
ORIGIN      
        1 gctggcgacg cagaacccca tcgagtacga aggcacctac ccgctcccgg aggcgcagct
       61 cgaccgtttc atgttcaagg tcaaagtcaa tccgccgacc gccgacactt tggacgagat
      121 cctgcgtcgc accacttcgg gcctgatcac ggaagtcccc agggtcgtcg acgcggaaac
      181 ggtgagctcg atgctggcca cgactccaac tgtcccgatc gcggcccatc tcatccgata
      241 cgtgtctctc atcatcgagg ccacccaccc cgacgcggaa tccgccccgg cgtcggtgcg
      301 cagatacgtg cgctgggggg catcgccccg cggcgggcag gcgatggtgc tggcagcaaa
      361 ggcgaccgcc ctgctcgagg gccggcccca cgtcaccgcg gaggacatcc gctgggccgc
      421 gacctcggcg ttgcgtcacc ggctggtcct cggctacgaa gccaccgccg agggggttag
      481 atcggagtcc atcatcggcg atgtgatcaa acaggtatcc gagccgaagc cggacatccg
      541 aggggttcct tgagtcttct cagcccgtca ttcatggcgc ggctggagag cctccaactc
      601 cacagccgac accgtctcgt gggtcggttt ggtggcgagc atcgctcgaa gaggtatggg
      661 aacaccgtcg acttcgccga cttccgcgag taccacccgg gagacgactt tcggcggatc
      721 gactaccacg tgctggcccg tctcgatcag gtgctgatca aattgttcga ggccgacgac
      781 gaggtcaccg tgaggatcct gatcgacacc tcggcctcga tggcggtgga gggaaagctg
      841 catcaggcaa agcgtctcgc agcggcgctc gggttcgtag cgctcaccgc ccacgacgcg
      901 gtcagcatcc acacctttcc cgccgatggc ccacccccga gattcgcggg ccgggccctg
      961 gtgccggacc tgttcgccta tctcgagtcc ctgcagccca agggtctcac tccgttcgcc
     1021 caggccgcag gtcatctcct cggccggtcc gggccggcgg ggatcacgat agtgatcagt
     1081 gacttgctca cacccgagtg gaggtcgctg atgcggctgc gcgcctccgg ctcggatctc
     1141 accgtcttcc acctgctcgc cgaggaggac gtggcacccg agttcgccgg ggacctgatg
     1201 ctggtcgatc gtgaaggtgg cgaacggctg accgtgagcg tcaccgacga agttgcccgg
     1261 tcgtttcgtg tccgggtggc ggaatggcag aacctggtcc gctccaacag ccggggggtc
     1321 ggcgcggtgt acatgccggt ccgcccgacc caggacatcg agaggctgct cctgacctcg
     1381 tggcgtgacg ccggagtgct ccggtgacac tgctggcgcc gatcgggctc gccgcgctgc
     1441 tgctgggggc cgcggtggtg atcctgcaca tgctcacccc gcgccggccg ccgaccccgg
     1501 tctcctcact gcttcattgg gacggcctca agcattcgat cacggccgcc gagccatggc
     1561 agaagctccg ctggagcctc ctgttgatcc tgcaactcct ggcagtcgcc ctgttcgccc
     1621 tcgccctgtc tcagccggct cgggtcgagg tggccgatct cgccgagcac accgtgttca
     1681 tcgtggacgc ctccggttcg atgtcggcca tcgacggctc cccggaccgt ctcgccgaag
     1741 cggtccagcg cgccgcggaa ctccaggagg acattccaca gggtggcgcc tcgagtgtgg
     1801 tggtcgcctc caccaacccg gccgtcctgc tccaggagag cacgagccct gatgagttcc
     1861 agcgagcaat ggcctcgatc agggcctcgt ccggagacgc cgactacgaa gcggcgtttg
     1921 ccctcgccga gtcgctcgtg tcggcggaga g
//
LOCUS       DATITW010000263         1610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_573993, whole genome shotgun sequence.
ACCESSION   DATITW010000263 DATITW010000000
VERSION     DATITW010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573993"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VLS86_03740"
     CDS             <1..277
                     /locus_tag="VLS86_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49635.1"
                     /translation="IFGGYLVGVELLGLSPGTYFGEMKTFVTMDDLMTGYWKAISFGI
                     IVPWVCTYKGFNCGHGAAGVARATTQAVVLTSILILVWDYFLGSVLP"
     gene            274..1026
                     /locus_tag="VLS86_03745"
                     /pseudo
     CDS             274..1026
                     /locus_tag="VLS86_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1040..1480
                     /gene="mlaD"
                     /locus_tag="VLS86_03750"
     CDS             1040..1480
                     /gene="mlaD"
                     /locus_tag="VLS86_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HSO49636.1"
                     /translation="MKRSVLDLSVGIFVIIGLVALGWMSVKLGRVDLLGSRGYVISAD
                     FPSVGGLKAGSAIEIAGVEIGRVDKILLQDYQARVVMVIQPGIKLQDDSIASIKTKGL
                     IGERYVRISPGGSDKIIGPNGRIREVEPPVDIEELLSKYIFGKV"
BASE COUNT          272 a          517 c          545 g          276 t
ORIGIN      
        1 catcttcggt ggctacctgg tcggtgtcga gctcctcggg ctctcccccg gcacctactt
       61 cggcgagatg aagaccttcg tcaccatgga cgacctcatg accgggtact ggaaggcgat
      121 ctcgttcgga atcatcgtgc cctgggtttg cacgtacaag ggcttcaact gtggccacgg
      181 cgccgccgga gtcgcgcggg ccaccaccca ggcggtggtc ctgacctcca tactcatcct
      241 ggtctgggac tacttcctgg ggtccgtgct gccgtgatcc gggtccaggg cctccacaag
      301 cgcttcggca gccagccggt cctgcgcggc atcgatctcg acatcgccac cggcgagatc
      361 atggtggtca tcggccggag cggcggcggc aagtcggtcc tgctcaagca cctcatcggg
      421 ctgctccgac ccgattcggg cacggtcgcg gtggacggca ccgagatcac ccgtctccgc
      481 ggcggcgagc tggaccgggt acgcgagcgc tacggggtgg tcttccaggg aggcgccctc
      541 ttcgactcca tgtcggtcgc cgagaacgtg gccttcccgc tccgagagaa atcccgcctt
      601 ggacacgccg agatcaggga acgggtggag gaaaagctct agcaggtcgg gctgtccgac
      661 atgggccaca agaaccccgc cgagatctcg ggcggcatgc gcaagcgcgt ggcgatcgcg
      721 cgggccctgg tcaccgagcc cgagatcgtc ttcttcgacg agccgaccac cggtctggat
      781 ccgatcctgg tcaacaccat ccaccatctc atcctggagc tgcaccgcaa gttccgcttc
      841 accgcggtca tggtcagcca cgagattccc gaaatctttg agatcgcgga ccgcgtcgcc
      901 atgctccatg agggcgtgat catcgaggtc gggccgccgg aggcggtgcg cgcgtccgcg
      961 aaccccatcg tccagcagtt catccgcggg gacatcgaga cggtgcccgc ggggcagcgg
     1021 ggctagccgg gagaacagaa tgaaacgttc cgtactggat ctgagcgtcg gcatcttcgt
     1081 gatcatcggg ctcgtcgcgc tcggctggat gtcggtgaag ctcgggcgcg tggatctcct
     1141 gggcagtcgg ggctacgtga tcagcgccga tttcccgtcg gtgggaggcc tcaaggccgg
     1201 gtccgcaatc gagatcgcgg gcgtcgagat cgggcgggtc gacaagatcc tgctgcagga
     1261 ctaccaggcg cgggtggtca tggtcatcca gcccggcatc aagctccagg acgattcgat
     1321 cgcctccatc aagaccaagg ggctgatcgg cgagcgatac gtacgcatca gcccgggcgg
     1381 gtcggacaag atcatcgggc ccaacggacg gatccgcgag gtcgagccgc cggtcgacat
     1441 cgaggagctg ctctccaagt acatcttcgg caaggtctag actcactctc caaggggagg
     1501 cattcgatga actggcttcg catgggggca atggtgctgg cgggcgcgct ggtggtcggg
     1561 gtgagcgcgg cgcccgccgc ggcgggagtg cccaccgatc agctcaaggg
//
LOCUS       DATITW010000264         1773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_413717, whole genome shotgun sequence.
ACCESSION   DATITW010000264 DATITW010000000
VERSION     DATITW010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413717"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VLS86_03755"
     CDS             complement(<1..550)
                     /locus_tag="VLS86_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49637.1"
                     /translation="MERITDNGDRAGAFKRLLYVLLPLLALMLVTLGVAYISDVPVTD
                     LLRDPSAVLDGPWYAGLFSTTGIALWAAAGAMCLLALTAEPSSKLRSLLVAGAVISFM
                     LGADDGYLIHETVKNEIGIPSPVTIAAYGVIAIALLWPVWGFLRGRPDFVVFVAAVAL
                     FAISAILDGAAEAGLPTPPYSSI"
     gene            732..>1773
                     /locus_tag="VLS86_03760"
     CDS             732..>1773
                     /locus_tag="VLS86_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49638.1"
                     /translation="MTNTHFGGSPSRSPYLRRSPERRLLLGLIGLAVVVAVVIGFANR
                     ADAATTTLTGTNNRGSLTVAAGDTLVFDPNNDTTLNMTGNLIVRGTLIMKPANGNVDH
                     LIRFNGINESNFVGGGMDVIGTDTGLWVMGSGKIILQGESKPAWSYTWQSSWAGDEVR
                     AAPNSPGSYSNFPLVTSTPAKNALGYSAELLNLTRNVRVEGTPSGYSHVFIRSTTPST
                     IKNAAFRYMAPDPSNVANAEDDRTGRYGLHIHMSGNGSRGTIVDGLVIRDTKAHAFVP
                     HGSHGITFRNTIAYNVRGEAYWWDEPASGVQNNSNDILWDRTVAALVREASGGENHRL
                     AAYFLGAGNNIAV"
BASE COUNT          352 a          582 c          567 g          272 t
ORIGIN      
        1 gtatcgacga gtaggggggc gtgggcagac cagcctcggc tgctccgtcg aggatcgccg
       61 aaatggcgaa cagcgctacg gcggcaacga acacgacgaa atccggacgg ccgcggagaa
      121 atccccagac gggccagagc agggcaatgg cgatcacccc gtaggcagcg attgtgaccg
      181 gtgatgggat gccgatctcg ttcttcaccg tctcgtggat caggtagccg tcgtcggcgc
      241 cgagcatgaa gctgatcacc gcgccggcga cgagcaggga ccgtagtttc gaagaaggct
      301 cggcggtcaa ggccaggagg cacatggcgc cggcggccgc ccatagcgcg ataccggtcg
      361 tggagaaaag ccctgcgtac cagggcccgt cgagaaccgc gctcgggtct cggagaaggt
      421 cggtcaccgg gacatcggag atgtatgcca ccccgagcgt gaccagcatc agagccagca
      481 gcggcaacaa cacatacagc aagcgcttga acgccccggc gcgatcacca ttgtcagtga
      541 tccgttccat gcgacaacct cccggtattg cgacagccta ccccggggtc ggttgcggtc
      601 cagggccagt cctcactgtg cgtggtggat ggtggtaggg ccgtggtcgt ggttaagctg
      661 acctttcgcg gttgtcggtc gtggtgggcc gacacaggac tggcagcatc caaccaggag
      721 ggcacacgcc agtgaccaac acacatttcg gggggagccc tagtcgctcg ccctatctcc
      781 gacggtcacc tgagcgacgt ctgcttctcg gactcatcgg cttggcggtg gttgttgccg
      841 tggtcattgg gttcgccaac cgggccgatg cggcgaccac caccctcacc ggcaccaaca
      901 accgcgggtc cctcacggtg gcggccggtg acaccctggt cttcgacccc aacaacgaca
      961 cgaccctgaa catgaccggg aacctgatcg tgcggggcac cctgatcatg aagccggcca
     1021 acgggaacgt ggaccatctg atccggttca acgggatcaa cgagtcgaac ttcgtcggtg
     1081 gcgggatgga cgtgatcgga accgacaccg ggctctgggt gatgggctcg gggaagatca
     1141 tcctccaagg cgagtccaag cctgcatggt cgtacacctg gcaatcgtcc tgggccggcg
     1201 acgaggtcag ggcggcgccg aactcaccgg gcagctacag caacttcccg ttggtcacct
     1261 cgaccccggc caagaacgcc ctgggctaca gcgccgagct gctcaacctg acccgaaacg
     1321 tgcgggtcga gggcacgccc agcggttaca gccacgtctt catccggtcg accacaccct
     1381 ccaccatcaa gaacgccgcc ttccgctaca tggcgcccga tccttcgaac gtggccaacg
     1441 ccgaggacga caggaccggt cgttacggcc tccacatcca catgtccggg aacgggagcc
     1501 ggggcacgat cgtcgatggg ctggtcatcc gggacaccaa ggcccacgcc ttcgttcccc
     1561 acgggtcgca cgggatcacc ttccgcaaca ccatcgccta caacgtcagg ggtgaggcct
     1621 actggtggga cgagcccgcc agcggggtgc agaacaactc caacgacatc ctctgggacc
     1681 gcaccgtggc ggccctggtc agagaggcgt cgggaggcga gaaccatcgt ctcgccgcct
     1741 acttcctcgg cgccggtaac aacatcgcag tca
//
LOCUS       DATITW010000265         3874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_734238, whole genome shotgun sequence.
ACCESSION   DATITW010000265 DATITW010000000
VERSION     DATITW010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734238"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VLS86_03765"
     CDS             <1..456
                     /locus_tag="VLS86_03765"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HSO49639.1"
                     /translation="DEATVTKWTQAAQSLAMKLQASENNVNSLKEQYQFAVGQAEEAK
                     EAVQTNAMNLQELSAKRMEMLGSLEQAKMQESVNSAVEAMSRGVTDDMPSLDQVEQKI
                     EQRKAEAMAKAELREATPEGAEAELRQAINTTQADAKLDELRAELGLQS"
     gene            complement(495..1964)
                     /locus_tag="VLS86_03770"
     CDS             complement(495..1964)
                     /locus_tag="VLS86_03770"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSO49640.1"
                     /translation="MIADRYELIEQIGTGGMATVWRATDTLLGRSVAVKRLLPHLALD
                     PAAAERFKREAQAAAGLSHPGIVTVFDTGEDDDGPFMILELIEGETLAARLASVGPLA
                     PALIVDIVSQVGSALDHAHGLGVVHRDIKPANLILEPDGRVRLADFGIARTVDDPTSI
                     TATGELVGTISYMAPELLEGGSPGPASDVYSLAAITYEMLAGTPPFSAETPAATIEAV
                     RRAEPASLAGRVPDEMAAAVTAGMSRDLSRRPPSAGMFASGLVGAATLVLGQGGAAAG
                     LSSQSAAPSSFLGSEDPTVVTSRQPPPPPPSAAEPVLAKGGRSRWPLLLVLLGVLALA
                     AAAMTNDLSPTGSAGTLDDLGAAEDPTTIPTTTTTVPTTTTTVLPSTTTIPTTTTVAD
                     NPETVAVEVETLLASLQPPQFSPRDVRRIEDRLDRVMEEWESDDRDDLMRELERAFEA
                     VSDLEQSADRDQLNQLFIQLAELMGFEVDQGGQGGDDDD"
     gene            complement(2021..3415)
                     /locus_tag="VLS86_03775"
     CDS             complement(2021..3415)
                     /locus_tag="VLS86_03775"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HSO49641.1"
                     /translation="MLFVSTEPVIFISHRHSDREIAETLARFVRNKTAGNGRVYLSSS
                     PDFEGPRLGQPLNMELKRALAESELVILVYTSDRLDWSYCMWECGVAVDPKDERHTSV
                     VVVQCTADEPKPFGDQLRVDARDLDSIQAFVKALLAGTDFFPLRDAPITGFAAEGNEI
                     REFAAELHSSLADVIPTGVGADRSTPTAPYLRVRLGSQAADQIRTSYLAGDEGQSMKI
                     VESEGLIVEQDGAGALFGMKLGAESTLGDVLADWRSDTAHTGQEARWFEALTDQIEAA
                     LVGRLRPVKWAAYQSVKGQTDVPYVAGSRQAPDGVEFDVYMVPIAPRPVPVRERMLPI
                     DQTYHKNAATEPLDEILLSSLIREMGAYKATRLPILDGRTARSIVHRSTINEFLAERA
                     LETGTVEGLTLMDLLADHADALEHSYVEVPPEATIEEAMDSMAAAPGCQDVYVTDESG
                     AVIGWITNVMFIQD"
     gene            complement(3470..>3874)
                     /locus_tag="VLS86_03780"
     CDS             complement(3470..>3874)
                     /locus_tag="VLS86_03780"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HSO49642.1"
                     /translation="YAISKFDQGHVRVGVRRAAEMLAESGASEVITSSVRPARWIPGS
                     GSFDSFLAEADAIGYGPNRTSYFSFHQMGSARMGSNPAISVVDSENQAHDTSGLYVMD
                     GSCFPTASGVNPMLSIAAIAHRGATILANRIG"
BASE COUNT          687 a         1242 c         1292 g          653 t
ORIGIN      
        1 gacgaggcga cggtcaccaa atggacccag gcggctcagt cgctggcgat gaagctgcag
       61 gcatccgaga acaacgtcaa ctcgctcaaa gagcagtacc agttcgcggt gggtcaagcc
      121 gaggaggcca aagaagcagt ccagaccaat gcgatgaacc tccaggagct gtcagcgaag
      181 cggatggaga tgctgggctc gttggagcag gcaaagatgc aggagtcggt gaattcggcg
      241 gtcgaggcta tgtcccgcgg cgtcaccgac gacatgccca gtctcgatca ggtggaacag
      301 aagatcgagc agcgcaaggc cgaggccatg gccaaggccg agctacggga agccacgccg
      361 gagggagcag aggccgagct gcgccaggcg atcaacacca cgcaggcgga cgcaaagctc
      421 gacgagctca gggcggagtt gggtctccag agctgatcgc agacgagtcg cggttctggt
      481 gacgacgccc ggcgtcagtc gtcgtcgtca cccccctgac ccccctggtc gacctcgaat
      541 cccatcagtt ctgcgagttg gatgaacaac tggttcagct ggtcgcggtc tgccgattgc
      601 tccagatcgg acacggcctc gaaggctcgc tccaactccc gcatcaagtc gtcgcggtcg
      661 tcgctctccc attcctccat cacccggtcg agacgatcct caatccgtcg cacatccctg
      721 ggtgagaact gtggtggctg gagactggcg agaagagtct cgacctcgac ggccacggtc
      781 tccgggttgt cggccacggt cgtcgtcgtc gggatggtcg tcgtgctggg gagaacggtg
      841 gtggtagtgg tgggaacggt tgttgtcgtg gtcgggatcg tcgtcgggtc ctcggcggcc
      901 cccaggtcgt cgagtgtccc agcactcccc gtcgggctca gatcgttggt catggcggca
      961 gcggccagag ccagtacccc gagtaacacc agcagcagcg gccagcgtga tcgtcctccc
     1021 tttgcgagca ctggctcggc cgcggagggt ggtggcggcg gaggctgacg actcgtgacc
     1081 accgtgggat cctcggagcc gaggaaagag cttggtgctg cagactgcga gctgagaccg
     1141 gcagctgcgc ccccctgacc gaggaccagg gtcgccgccc cgaccaggcc ggaggcgaac
     1201 atcccggcgc tgggtggtcg gcgggacagg tccctggaca tccccgccgt caccgccgcc
     1261 gccatctcgt cggggaccct tccggccaga ctcgcgggct cggcacgacg aaccgcctcg
     1321 atggtggcgg ccggggtctc cgccgagaat ggtggtgttc cggccagcat ctcgtaggtg
     1381 atggcggcca gggagtagac gtccgaggcc ggccccggag acccgccttc gagcaactcg
     1441 ggcgccatgt aggagatggt tcccaccaac tccccggtgg cggtgatcga cgtcgggtcg
     1501 tcgaccgttc tcgcgatccc gaagtcggcg agacgcaccc ttccatcggg ttcgaggatc
     1561 aggttggccg gcttgatatc acgatggacc actcccaggc cgtgggcgtg gtcgagagcg
     1621 ctgcccacct gcgagacgat gtcgacgatc agagcggggg caagcgggcc gaccgaggcc
     1681 agcctggcgg cgagggtttc gccttcgatc agctcgagga tcatgaacgg tccgtcatcg
     1741 tcttcgccgg tgtcgaacac ggtcacaatc ccgggatggc tgagaccggc agcggcctgg
     1801 gcttcacgtt tgaacctctc cgccgccgcg gggtccagtg ctagatgggg gaggaggcgc
     1861 tttaccgcaa ccgatcggcc gagaagcgtg tccgtggccc tccacaccgt ggccatcccc
     1921 ccggtcccga tctgctcgat gagctcgtag cggtcagcaa tcacccaaac aggttaagga
     1981 gaaggtcaaa gtagggggag agccgcctga tggcgagacg tcagtcctgg atgaacatga
     2041 cgttcgtgat ccacccgatc actgccccgg actcgtcggt gacatagacg tcctggcaac
     2101 caggcgcggc ggccatactg tccatcgcct cctcgatcgt cgcctccggt ggtacctcga
     2161 cataggaatg ctccagggcg tcggcgtgat cggccagaag gtccatcagg gtgagaccct
     2221 ccacggtccc ggtctcgagc gcccgctcgg ccaggaactc gttgatcgtc gaccggtgca
     2281 cgatcgatct ggcggttcga ccgtcgagga tgggcagccg cgtcgccttg tacgctccca
     2341 tctccctgat cagcgacgag agcaagatct catcgagcgg ctcggtggcc gcgttcttgt
     2401 gatacgtctg atcgatgggc agcatccgct cccgcaccgg aaccggtcgg ggggcgatgg
     2461 gcaccatgta cacgtcgaac tccaccccat ctggggcctg gcgggaaccg gcgacataag
     2521 gcacatcggt ctgacccttc accgactgat aggcggccca tttcaccggg cgcagacgcc
     2581 cgaccagagc cgcctcgatc tggtcggtga gcgcctcgaa ccagcgtgcc tcctgtccgg
     2641 tgtgcgccgt gtcgctgcgc cagtcggcta gcacgtcgcc caatgtcgac tcggctccca
     2701 gcttcatccc gaacaaggca ccggctccgt cctgctccac gatcaacccc tcggactcca
     2761 cgatcttcat gctttggccc tcgtcgccgg ccagatagga ggtgcggatc tggtctgcgg
     2821 cttggctgcc gagccggact ctgaggtagg gcgcggtggg cgtgctccgg tcggcgccca
     2881 cgccggtggg gatgacgtcg gcgaggctgg aatgcaactc ggcggcaaac tcccggatct
     2941 cattgccctc agcggcaaat cccgtgatag gcgcgtcacg gagagggaag aagtcggtgc
     3001 ccgccaacaa cgctttcacg aaggcctgga tggagtcgag gtcgcgggca tcgacccgta
     3061 gttgatctcc gaacggcttt ggctcgtcgg cggtgcactg caccaccacc accgaggtgt
     3121 gacgctcgtc cttggggtcc accgcaaccc cgcactccca catgcagtaa gaccaatcca
     3181 accgatcgct ggtgtagacc aggatcacca gctcggattc ggccagggcg cgcttcagct
     3241 ccatgttgag cggctggccg aggcgtggtc cctcgaaatc tgggctcgag gagaggtaga
     3301 cgcggccatt ccccgccgtc ttgttcctta cgaaacgggc cagcgtctcc gcgatctcgc
     3361 gatccgagtg ccggtggctg atgaagatga caggctcggt agacacgaag agcacctctg
     3421 attcgatcct acgcggcagc actcggcccg tgggccgact cgatgcgatt cagccgattc
     3481 tgttggcgag gatcgttgcc ccgcggtggg caatggcggc gatggacaac atcgggttga
     3541 ccccggaagc ggtggggaag cacgagccgt ccatcacgta cagcccgctg gtgtcgtggg
     3601 cctggttctc ggagtcgacc accgagatgg cggggttgct gcccatgcgg gcgctgccca
     3661 tctggtgaaa cgagaagtag gaggtgcggt tcggcccgta cccgatggcg tcggcctcgg
     3721 cgaggaacga atcgaatgag ccactgcccg ggatccagcg agcaggtctc accgacgagg
     3781 tgatcacctc gctggccccc gactcggcca acatctccgc agcccgtctc accccgaccc
     3841 ggacgtggcc ctggtcgaac ttggagatgg cata
//
LOCUS       DATITW010000266         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_734270, whole genome shotgun sequence.
ACCESSION   DATITW010000266 DATITW010000000
VERSION     DATITW010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734270"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..1786
                     /locus_tag="VLS86_03785"
     CDS             329..1786
                     /locus_tag="VLS86_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSO49643.1"
                     /translation="MIAKKSKMYIGGKWVDSTSGETFDDLNPYTGDVYAQVPAGTPED
                     ARQAIDAAKAAFPEWSKTPPGERRAIFLKAADIMERRSDELVKAMMEEVGGTIGISMF
                     QMHFVPGLYRTAASAAYEVKGEIIPADHKDSLFLAMRQPAGVVACVAPFNVPYILGSR
                     SFTLPVAYGNTAVLKPSEDAPLTGGLLLAEIFEEAGLPAGVLNVITGTREAASAIGDE
                     MISNPAVRRISFTGSTEVGRIIAEKAGRHLKRAVLELGGKDPLIILGDADVDYAVDAA
                     VWGSFLHQGEICMSTERIIVEKSVAKEFIDKLKRRAEELPVGDPTNPAMAIGPLINQR
                     AVDKVHAHVEEAVAGGAHLETGGRYDNLLYYPTVLTEVDPDMRVFREQTFGPVAPVVV
                     VKDRDEALAVANNSDYGLSAGIITNDFTRALDMALRLETGMVHIGDQTVNDEPQAPFG
                     GVKGSGYGRFGGQAALDEFTELRWINIQTRPRTFP"
     gene            complement(1798..>2267)
                     /locus_tag="VLS86_03790"
     CDS             complement(1798..>2267)
                     /locus_tag="VLS86_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HSO49644.1"
                     /translation="SMSVLDGSEIVYVARVPTKRIMTISLALGSRLPAYPTSMGRVLL
                     AGLTDQEIDDYLAKTRLEQLTPHTITDLVELRKVIEVVRSEGYAVVDQELEEGVRSIA
                     APITTGRGKVVAAMNISGHSSRVTLEKMLEDFRPRLLSTARTISESTGSLPWT"
BASE COUNT          433 a          725 c          740 g          369 t
ORIGIN      
        1 ccagccgtcg tagtcgtggt gcccgcctcc acgcaggcgg tcgcgacgag caccacgagc
       61 gccgctgcca taaccgtgac cgatttcggt ctttgtctca tcctcatcct cctcctcgtc
      121 caggtaggga cgacgccgca ccgggactcg gggaaagact tagcctcccg gcccatcgag
      181 gggtcgcctc gatgtgcgca tagcgcccat ccgtgcgagc agcgtacact ggcggaacga
      241 tgtctgcccg agcgatcgtg cgcgagtatc agggaggcgt gcacctggcc gaacggaagc
      301 cggacagccc caacgaggag gagaggcgat gattgccaag aagtcgaaga tgtacatcgg
      361 aggaaagtgg gtcgactcca cctcggggga aacgttcgat gacctgaacc cctacaccgg
      421 cgacgtgtac gcccaggtgc cggcagggac tcccgaagac gccagacagg ccatcgacgc
      481 ggccaaggcg gccttcccgg agtggtcgaa gactccaccg ggggaacgcc gggccatctt
      541 cctcaaggca gccgacatca tggagcgacg atccgacgag ctggtgaagg cgatgatgga
      601 ggaggtcggg ggaaccatcg gcatctccat gttccagatg catttcgtcc ctggcctgta
      661 ccgcacggcg gcctccgcgg cctacgaggt gaagggtgag atcatccccg ccgaccacaa
      721 ggactccctc ttcctcgcga tgcgccagcc ggcgggagtg gtcgcctgcg tcgctccttt
      781 caacgtcccc tacatcctgg gcagccgttc gttcaccttg cccgtggcct acgggaacac
      841 cgcggtgctc aaaccctcgg aagatgctcc cctcaccggt ggcctgctcc tcgccgagat
      901 cttcgaggag gccggactgc cggccggtgt gctcaatgtg atcacaggaa cacgcgaggc
      961 ggcatcggct atcggcgacg agatgatctc caacccggcg gtgaggcgga tctccttcac
     1021 cggctccaca gaggtgggca ggatcatcgc cgagaaggcg ggcaggcatc tgaagcgagc
     1081 ggtcctcgag ctgggcggca aggaccccct gatcatcctc ggcgacgccg acgtcgacta
     1141 cgcggttgat gccgcggtgt ggggttcttt cctgcaccag ggcgagatct gcatgtccac
     1201 cgagcggatc atcgtggaga agagcgtcgc taaggagttc atcgacaagc tgaagagaag
     1261 ggccgaggag ctgccggtgg gcgacccaac caatcccgcc atggcgatcg gtccgctgat
     1321 caaccagcga gccgtcgaca aggtccatgc ccacgtcgag gaagcggtgg caggtggtgc
     1381 ccacctggaa accggaggga ggtacgacaa cctcctctac taccccactg tcctcaccga
     1441 agtcgatccg gacatgcgtg tctttcgtga gcagaccttt gggccggtgg ccccggtcgt
     1501 agtggtgaag gaccgggacg aggccctggc cgtggccaac aactcggact atgggctatc
     1561 cgccgggatc atcaccaacg acttcaccag ggccctggac atggcgcttc ggctcgagac
     1621 cgggatggtc catatcggcg accagaccgt gaacgacgag ccccaggcgc cgttcggcgg
     1681 ggtcaagggg agcggatacg gccgctttgg cggccaggcc gcgctcgacg agttcacaga
     1741 gctgcgctgg atcaacatcc aaacccggcc ccgcactttc ccctgatagc ggcccaatca
     1801 ggtccaggga agggagcctg tgctctcgct gatcgtgcgc gcagtggaga gcagccgggg
     1861 tcggaagtcc tccagcatct tctccagggt gactcgggag gaatgaccgg agatgttcat
     1921 tgccgcaacc accttgcccc gcccagtcgt gatcggggcg gcaatggaac ggaccccttc
     1981 ctcgagctct tggtcgacca cggcgtagcc ctcgctgcgg accacctcga tcaccttgcg
     2041 aagctcgacc aggtcggtga tggtgtgcgg cgtgagctgt tccaaccggg tcttagcgag
     2101 gtaatcgtcg atctcctggt cggtgagccc ggcgaggagg acccgcccca tcgacgtggg
     2161 ataggcaggg agacgtgacc ccaaagcgag ggagatggtc atgatccgct tggtagggac
     2221 cctggccacg tagacgatct cggagccatc cagcaccgac atcgatg
//
LOCUS       DATITW010000267         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_373558, whole genome shotgun sequence.
ACCESSION   DATITW010000267 DATITW010000000
VERSION     DATITW010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373558"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..489
                     /locus_tag="VLS86_03795"
     CDS             280..489
                     /locus_tag="VLS86_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HSO49645.1"
                     /translation="MTEILLQAPEIHCDHCKVSIEGAVGSLEGVESVDVAIADATVRV
                     DFDETRLEVSSIKKAIEEQGYAVFG"
     gene            527..>1538
                     /locus_tag="VLS86_03800"
     CDS             527..>1538
                     /locus_tag="VLS86_03800"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HSO49646.1"
                     /translation="MTCATCAVRIERILGRQEGVEAASVNLAGASALVRVSDDVDVGV
                     LETAVDKLGYRIALHEGGDAPRNLVEHYHDDEDAQWRRFWIAAVLTLPAVLLAMLGPD
                     VFWNRLLQGILVTPVVIWVGWQFHRVAAKQARHLSANMDTLISLGSLAAYGYSIWALF
                     SDEHVFFETAGVIITLITLGRAFEARAKGRASDAVHRLLELGAREARVVVDGTEALIP
                     TGSVIPGDLMVVHPGETIPTDGVIETGASAVNESMLTGESLPVDKQAGDVVYGATINQ
                     TGRLTVRATAIGADTALAAIVRMVEQAQGSKAPIQRLADRVSAVFVPVVILVSIVTTA
                     TWL"
BASE COUNT          269 a          498 c          508 g          263 t
ORIGIN      
        1 accaccagac tttcccaata caacaaggcg gagtactgcc atgctcatgc ccccgtccgg
       61 tttccccggg tgcggggcaa gatcatggac gggcaggacg cctgagagaa tctggccggg
      121 acaggtcctc tccccctccc cgtcccgtgc cagacaaccg agagcctcgg cgcaagccgg
      181 ggctctctac ttgtccggga caaccggcgg cgtctcgact atcggctccc gtcgccacgt
      241 cggaagcact gctccggacc ggacgtagaa tcaggtgaaa tgacagagat cctgctgcag
      301 gcaccggaga tccattgcga ccactgcaaa gtcagcatcg agggcgcggt tgggtcgctc
      361 gagggggtgg agtcggttga tgtggccatc gccgacgcca cggttcgggt cgacttcgac
      421 gagacccggc tcgaggtgag ctcgatcaag aaggccatag aggagcaggg ttacgccgtc
      481 ttcggctgac cgcggctccg agaccatcct gttcgacgta gatgggatga cctgcgccac
      541 ctgcgcggtg agaatcgaac gaatcctcgg ccgtcaggag ggtgtcgagg cggccagcgt
      601 caacctcgcc ggggcatcgg cattggtccg tgtgagcgac gacgtcgatg ttggcgtcct
      661 cgagaccgcc gtcgacaaac tcggctatcg gatcgcactc cacgaaggag gcgacgcccc
      721 gcgcaacctg gtcgagcact atcacgacga cgaggacgcg cagtggcgga ggttctggat
      781 cgccgcggtg ctcaccctgc ccgcggtctt gctggcgatg ctcgggcccg acgtcttctg
      841 gaaccggctg ctccaaggca tcctcgtcac cccggtcgtg atctgggtcg gatggcagtt
      901 ccatcgcgtg gcggcgaaac aggcccgtca tctcagcgcc aacatggaca ctctgatctc
      961 gctcggatct ctcgccgcct acgggtactc gatctgggcc ctgttcagcg acgagcacgt
     1021 cttcttcgag accgccggtg tgatcatcac cctgataacc ctgggccggg ccttcgaggc
     1081 gagggccaag gggcgggcat ctgacgcggt ccaccggctc ctcgaacttg gggcaaggga
     1141 ggccagggtc gtggtcgacg ggacggaggc actcatcccc accgggtcgg tgattccggg
     1201 cgacctcatg gtggtgcatc cgggggagac gatcccgacc gacggcgtca tcgaaaccgg
     1261 cgcctcagcg gtcaacgagt cgatgctcac cggggagtct ctacccgtcg acaagcaggc
     1321 cggcgacgtc gtgtacggcg cgacgatcaa tcagaccggc cgtctgacgg tgcgagccac
     1381 tgcgatcggg gcggacacgg ccctggctgc gatcgttcgc atggtcgagc aagctcaggg
     1441 atccaaggct ccgatccaac ggctcgccga tcgggtttcg gcagtcttcg tccctgtcgt
     1501 gatcctggtc tcgatcgtga ccaccgcgac ctggctgg
//
LOCUS       DATITW010000268         1526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_453788, whole genome shotgun sequence.
ACCESSION   DATITW010000268 DATITW010000000
VERSION     DATITW010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453788"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VLS86_03805"
     CDS             complement(<1..308)
                     /locus_tag="VLS86_03805"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HSO49647.1"
                     /translation="MRRDEVPSTQDLARSELDRLPTVVIAERQTAGRGRTGATWLTAP
                     RALAASVALLMDVDDRRPLSLMAGLAAVRVVADVSLKWPNDVVAGDEKLGGILVERSG
                     P"
     gene            373..>1526
                     /locus_tag="VLS86_03810"
     CDS             373..>1526
                     /locus_tag="VLS86_03810"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014556696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HSO49648.1"
                     /translation="MGTEARIERLRELREEALHAGSQRAIARQHEQGKMTARERLEVL
                     LDKGSFQEMDQFVRHQSSGFGIEDKRPLGDAVVTGHGTIEGRPVFVFAEDFTVFGGSL
                     SKVVADKICKVLDMALDTGTPVIGLKDSGGARIQEGIASLDGYGRIFERNVRASGVVP
                     QISVIMGPCAGGAVYSPALTDFVFQVDQTSHMFITGPDVIKAVTGEEVTMEELGGASA
                     HASKSGVTHFVYPTGVDALEAVRYLMSFLPKNNMELPPYFAPTDRLDRIDDPLDSIIP
                     DSSNQPYDMVEILETVLDNGEFFEVHELWAGNIVIGLGRLDGHTVGVVANQPAVLAGT
                     LDITASEKAARFVRFCDAFNIPILTFVDVPGFLPGVDQEHNGIIRHGAKLL"
BASE COUNT          287 a          487 c          480 g          272 t
ORIGIN      
        1 ggaccggatc gctcgacgag gatcccgccc agcttctcgt ccccggcgac gacgtcgttg
       61 ggccatttca atgagacatc ggccacgact cggaccgccg ccaggcctgc catgagcgag
      121 agaggtcgcc gatcgtccac gtccatcaac aatgccaccg aggcagcgag agccctcggc
      181 gcggtgagcc aggttgctcc ggtcctcccc ctacccgccg tctgccgctc ggcgatcacc
      241 accgtcggca gccgatccag ctcggagcgg gccaggtcct gagtcgatgg aacctcgtca
      301 cgcctcaatt gcaaataggg tgtagccatc tcgtctcctg gcgagtcgaa aacgtgaggt
      361 tacggaggag ttttggggac cgaggcgcgg atcgagcggc tcagagagct cagagaggaa
      421 gcgctgcacg cgggaagcca gcgcgctatt gcgcgccagc acgagcaggg caagatgacg
      481 gcgcgggaaa ggctcgaggt tctcctcgac aagggctcct tccaggagat ggaccagttc
      541 gtccgacacc agtcgtccgg tttcgggatc gaggacaagc gaccccttgg tgacgcggta
      601 gtcaccggtc atgggacgat cgagggaagg ccggtgttcg tctttgccga ggacttcacc
      661 gtcttcggtg ggagcctgag caaggtcgta gccgacaaga tctgcaaggt gctcgacatg
      721 gcactcgaca ccgggacccc ggtgatcggg ctcaaggact cgggcggggc caggatccag
      781 gagggcattg cctccctcga tggatacggc cggatattcg agcgaaacgt cagggcttcg
      841 ggggtggtgc cccagatctc ggtgatcatg ggcccatgcg ccggaggtgc ggtctactcc
      901 cccgccctga ccgacttcgt gttccaggtc gaccagacca gccacatgtt catcaccgga
      961 ccggacgtca tcaaggcggt gaccggagag gaggtgacaa tggaggagct cggtggcgcc
     1021 tcggcgcatg cctccaagtc cggtgtcacc catttcgtgt atcccaccgg cgtcgatgca
     1081 ctggaagccg tcagatatct gatgtcgttt ctccccaaga acaacatgga gctccccccc
     1141 tacttcgccc ccaccgatcg cttggatcgg atagacgatc ctctcgattc gatcatcccc
     1201 gattcctcga atcagcccta cgacatggtg gagatcctcg aaaccgtgct cgacaacggg
     1261 gagttcttcg aggtccacga gctctgggcg gggaacatcg tcatcgggct cggtcgtctc
     1321 gacggtcaca ccgtcggagt cgtggccaac cagcccgcgg tgctggccgg cacactggac
     1381 atcaccgcct cggagaaggc agcccgcttc gtccgcttct gtgatgcgtt caacatcccg
     1441 atcctgacct tcgtcgacgt gccgggtttc ctcccgggtg tcgaccagga gcacaacggg
     1501 ataatccgtc atggagccaa gctgct
//
LOCUS       DATITW010000269         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_173219, whole genome shotgun sequence.
ACCESSION   DATITW010000269 DATITW010000000
VERSION     DATITW010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173219"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VLS86_03815"
     CDS             <1..380
                     /locus_tag="VLS86_03815"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HSO49649.1"
                     /translation="EYRFGLLASQLFRSARVVVDIGTQLEKAIPSDAPMHAGEVWDYD
                     IAVDYMEKVGLNARDVAESEVKRYLGWPAQAISYKVGEREILDIREQAVAKAGGSLDR
                     KDFHRRLLEAGAIRLDHLREAMA"
     gene            377..982
                     /locus_tag="VLS86_03820"
     CDS             377..982
                     /locus_tag="VLS86_03820"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HSO49650.1"
                     /translation="MKKTSFDLTVPDARDSYKLLSGLVVPRPIGWIGTVRSNGTYNLA
                     PFSFFNVVSTDPPVVMFAAGHHSDRPKDSSTLAEESGEFTVNIVSFELAPVMNLTSAS
                     LGAADDEFAVAGLTPVCGKGGAPMVAEAPANLECRVIEGISFGSNRQTRMVVGEVVAI
                     HVREDALDGTRVDPGVIDAVGRLSGSSYVTTRDRFDLPRPG"
     gene            complement(1013..>2145)
                     /locus_tag="VLS86_03825"
     CDS             complement(1013..>2145)
                     /locus_tag="VLS86_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HSO49651.1"
                     /translation="VVGLVLTLCVAMFSVEYTVKRGDTLGDIAREHDVSLSDLADAND
                     ISDPDRIRTGQVLVIPGEDGEPDRYHKVARGETLVRIASSYGANAATVAKANALSNPN
                     LIFPGQELLIPAKSGSGGRSSGGDGESQGSSNRAGSGSRSGQYHVVKRGETLESIADK
                     YSGVSADDIARANGITNGRIYTGTRLFLDGPSYVGSGGSGTGTYEVKSGDRLGDIAAR
                     FDTTISNLAKMNDLANVNRIRSGQALRVPGGSSWVCPVSNSRYFNDWGFPRGSSRYHE
                     GNDLFAGYGAKVRAPVSGTVELNRGPLGGLQFNLRGSDGVEYLGAHLDSSGKTGRVSA
                     GDVIGYVGTSGNAQGTSPHLHFGMYIDGLAINPYPTLRANGC"
BASE COUNT          359 a          670 c          720 g          396 t
ORIGIN      
        1 ccgagtaccg gtttgggctg ttggccagcc agctgttcag atcggccagg gtggtggtcg
       61 acatcggcac ccagctggag aaggcgatac cgtccgatgc cccgatgcat gccggcgagg
      121 tctgggacta cgacatcgcc gtcgactaca tggagaaggt cgggctcaac gccagggacg
      181 tcgccgagtc ggaggtgaag aggtacctcg gctggccggc tcaggcgatc tcctacaagg
      241 tgggagagcg tgagatcctc gacatccggg agcaagcggt ggccaaggcc ggtggcagcc
      301 tggatcgaaa agacttccat cgcaggctgt tggaggcggg agcgatccgt ctcgaccatc
      361 tgcgcgaggc gatggcttga agaagacgag cttcgatctt acggttcccg acgcccggga
      421 cagctacaag ctgctttccg ggctggtggt cccacgtccg atcgggtgga tcgggacggt
      481 gcgatccaac gggacctaca acctcgcccc gttttcgttc ttcaatgtgg tctcgaccga
      541 cccccccgtc gtcatgttcg ccgccggaca tcattcggat cggccgaagg actcatcgac
      601 cctggccgag gagagtggcg agttcaccgt gaacatcgtg tccttcgagt tggccccggt
      661 catgaacctg acctcggcca gcctgggggc ggcggacgac gagttcgccg tcgcgggtct
      721 gacccctgtg tgcggcaagg ggggggcccc gatggtggcg gaggcccccg ccaacctcga
      781 gtgccgggtg atcgagggga tcagctttgg atccaacagg cagacgcgga tggtcgtcgg
      841 cgaggtggtg gcgatccatg tccgggagga cgcactcgac ggcacccgtg tcgacccggg
      901 agtgatcgac gccgtcggcc ggctctccgg cagctcctat gtgacgacca gagatcggtt
      961 cgatctgccc agacccggtt gaggcgcccg tctttgagct cgggctgagc ggtcagcagc
     1021 cgttggctcg cagcgtgggg taggggttga tcgccagccc gtcgatgtac atgccgaaat
     1081 ggaggtgggg actggttcct tgggcgttgc ccgaagtgcc cacgtagccg atgacgtcac
     1141 cggcgcttac ccttcccgtc ttccccgaag agtcgaggtg ggcgccgaga tactcgacgc
     1201 cgtcgctgcc gcgcaggttg aactggagac cgccgagggg tcctcggttc agctccaccg
     1261 tccccgacac cggtgccctc accttggcgc cgtagcctgc gaacaggtcg ttcccctcgt
     1321 gataccgcga tgacccgcgt gggaagcccc aatcgttgaa gtagcggctg ttcgacaccg
     1381 ggcacaccca tgacgaccca cccggcactc tcagtgcctg cccggagcgg atccggttga
     1441 cgttggccag gtcgttcatc ttggccaggt tggagatggt ggtgtcgaat cgggcagcga
     1501 tgtctcccaa ccggtcccca ctcttcacct cgtaggtgcc ggtgcccgag ccgccgctgc
     1561 cgacgtagga aggaccgtcg agaaagagcc tggtgccggt gtagatcctc ccgttggtta
     1621 tcccgttggc gcgggcgatg tcgtctgcgg agacgccgga gtacttgtcg gcaatcgatt
     1681 cgagagtctc gccacgcttg acgacgtggt actgcccgga tctcgatccg gagccggccc
     1741 tattcgacga cccctggctc tcgccgtccc ctccggatga tctgcctccc gagcccgact
     1801 tggccgggat gaggagctct tggccgggga agatcagatt cgggttggac agcgcattcg
     1861 ccttggccac cgtggcggca ttggcgccgt aggaagatgc gattcggacc agcgtctcgc
     1921 cacgggccac cttgtgatac ctgtcgggct ccccgtcttc gcctggtatc accagcacct
     1981 ggccggtgcg gattcgatca ggatcggaga tgtcgttggc gtcggccaga tccgagagcg
     2041 agacatcgtg ctcacgggcg atatcaccca gggtgtctcc tctcttcacg gtgtactcga
     2101 ccgagaacat ggcgacacac agggtgagaa ccaagccaac gacca
//
LOCUS       DATITW010000270         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_93558, whole genome shotgun sequence.
ACCESSION   DATITW010000270 DATITW010000000
VERSION     DATITW010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93558"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1248
                     /locus_tag="VLS86_03830"
     CDS             163..1248
                     /locus_tag="VLS86_03830"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSO49652.1"
                     /translation="MDLAPQHSLISHAIEQGWARVMDAGTFIQGVEVKAFEEAYATFC
                     GVRHCVGLASGTDALEFSLRALGVGAGDEVIVPTNSFIASAAAVARAGATPVLTDVDP
                     EFLLIDPEDAGARVTSKTKAVIAVHLFGQLAPMEELGRAVGNKVLVVEDAAQAHGASR
                     NGVGAGGFGVAAGTSFYPWKNIGAYGYAGAVLTQDDEVARRVGTLRDHGSEVKYQHSE
                     LGFNSRLDTIQAVVLGAKLRFLRAWNQERREAAVRYDELLQKLGEVVRPKVLAGNEHV
                     WHLYVVRVPNRDEVLAKLQSVGIGAGIHYPTPIHLQGAFGDLGYRSGDFPVSEKASGE
                     ILSLPIYPGISPEQQERVVEQLRKALG"
     gene            1245..1859
                     /locus_tag="VLS86_03835"
     CDS             1245..1859
                     /locus_tag="VLS86_03835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HSO49653.1"
                     /translation="MTLDPSVFVHEEGLCESDDIGPRTRIWAFAHVMFGARIGADCNV
                     CDHAFIESGAIVGNGVTVKNSVLIWDKVTIEDDVFLGPNAVFTNDRNPRATVKKAHAD
                     LVPTLVRRGATVGANATIVCGVTIGQSAFVGAGAVVASDVPAYALVLGNPARRWGWMC
                     VCGERLPTELRCSCGRGYHEVRGVLQPDTYLEPIKATPYPEPGA"
     gene            complement(1856..>2368)
                     /locus_tag="VLS86_03840"
     CDS             complement(1856..>2368)
                     /locus_tag="VLS86_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49654.1"
                     /translation="RGPVIWSAPSRARGGSQARLERHALEERGIWGFEDCVAHNNSVN
                     GIFVWQNSDRRHVIDRFVAFHNGQAGIEHGAYLNSYVYRNVELVGNSLAGVLLHAESH
                     DQGSLRFENVTVDGRGITNFGFLGAESTLDGAPVIECGPSITGVTESDLSLDYSGDSI
                     EDRFEVRDSC"
BASE COUNT          439 a          703 c          800 g          426 t
ORIGIN      
        1 aagccgtggc aatcgagtca agcgaaaggc cgtatgaaca attcgagtca accttatgca
       61 gcgtcgatct cagcgagccc tccggaagcg ggtctcgcca tgtcggagcg aacaaggacc
      121 gagcgcggat cttccgctca cgccgatgcg tcgatcccac gcgtggatct cgcgccgcag
      181 cactccctca tctcccacgc catcgagcaa gggtgggctc gcgtcatgga cgcgggaacg
      241 ttcatccaag gagtggaggt gaaggcattc gaggaggcct acgcgacctt ctgcggcgtg
      301 cggcactgcg tcgggctggc gagcggcacc gatgccctgg aattctcgct gcgggccctc
      361 ggtgtcggag ccggagacga ggtcatcgtt ccgacgaatt cgttcatcgc gagcgcagcc
      421 gcggtggctc gagccggggc aacgccggtg ctcacggacg tggatccgga gttcctgctc
      481 atcgatccgg aagacgccgg cgctcgcgtc acctcgaaga caaaagcagt catcgcggtt
      541 catctgttcg ggcagctggc gccgatggaa gaacttggcc gagctgtggg gaacaaggtg
      601 ctcgtggtcg aggacgcagc gcaggcccat ggggcgagtc ggaacggcgt gggcgcgggc
      661 ggcttcgggg tggcagccgg taccagtttc tacccgtgga agaacattgg ggcatacggt
      721 tatgccgggg cggtgctgac ccaggacgac gaagtggcgc gaagggtggg cacgcttcgg
      781 gatcatggct ccgaggtcaa gtatcagcac tccgagctcg ggttcaactc acgcctggac
      841 accatccagg cggtcgtcct gggcgcgaag cttcggttcc tgcgggcctg gaaccaggaa
      901 agaagagagg cagcggtgcg gtacgacgag ctgctgcaga aacttggcga ggtggtacgt
      961 cccaaggttc ttgcaggaaa cgaacacgtc tggcatctct acgtggtgcg cgtccctaat
     1021 cgcgacgagg tgctggcgaa actgcagtcg gtaggtatcg gggccggtat ccattatccg
     1081 acgccgatcc acctccaggg agcattcggc gatctcggct accggtctgg agacttcccc
     1141 gtgtcggaga aggcgtccgg ggagatcctg tcgttgccga tttaccccgg gatcagtccc
     1201 gagcagcagg agcgcgtcgt ggagcaactt cggaaggcgc tcggatgacg ctggacccat
     1261 ctgtcttcgt ccacgaggag gggctgtgcg agtccgacga catcggtcct cgaacccgga
     1321 tctgggcttt cgcgcacgtc atgttcggtg cgcggatcgg ggctgactgc aacgtatgtg
     1381 atcatgcgtt catcgaaagc ggtgcgattg tgggtaacgg tgtcacggtg aagaatagcg
     1441 ttttgatctg ggacaaggtg acgatcgaag acgacgtgtt cctcgggccc aacgcggtgt
     1501 tcacgaacga tcgcaaccca cgcgcaaccg tgaagaaggc tcacgcggat ctcgtcccca
     1561 cgctcgttcg ccggggcgcg accgtcgggg cgaatgcgac aatcgtatgc ggtgtgacca
     1621 tcgggcagag cgcgttcgtc ggggccggag cggtcgtggc gtcggacgtc ccagcgtatg
     1681 cgttggtctt gggcaacccg gctcgccgct gggggtggat gtgcgtgtgc ggtgaacgac
     1741 ttccgaccga actccggtgc tcgtgtggtc gcgggtacca cgaggtgaga ggcgtgctgc
     1801 agccagacac gtacctcgag ccgatcaagg ctacgccgta tcctgaacca ggcgcctagc
     1861 agctgtcccg tacctcgaat cgatcctcga tcgagtcacc ggagtagtcg agggagaggt
     1921 cgctctcggt gacaccggtg atcgaggggc cacactcgat caccggcgca ccgtcgaggg
     1981 tcgactccgc tccgaggaac ccgaagttcg tgatcccccg accgtcgact gtgacgttct
     2041 cgaagcgaag gctgccttga tcatgggact cggcgtgcag aaggacgccg gcgagcgagt
     2101 tcccgacaag ttccacgttc cgatagacgt aactgttcag gtaggccccg tgctcgatcc
     2161 ctgcctggcc gttgtggaaa gcaacgaacc ggtcgatcac gtgacgccgg tcagagttct
     2221 gccaaacgaa gatcccattg acggagttgt tgtgcgcgac gcaatcctcg aacccccaga
     2281 tgcctctctc ctcgagcgcg tgtcgttcga gccgagcctg cgatccacct ctcgcccgcg
     2341 aaggggcaga ccatatcacc ggtcccct
//
LOCUS       DATITW010000271         1575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_534159, whole genome shotgun sequence.
ACCESSION   DATITW010000271 DATITW010000000
VERSION     DATITW010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534159"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VLS86_03845"
     CDS             <1..227
                     /locus_tag="VLS86_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011650648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49655.1"
                     /translation="IIGGASFLGGRGKVSHALVGALMIGVIRNSMNLANLDSFFQLIV
                     LGIVIIVAVESDVLRGHLENRLRLRQATRT"
     gene            224..988
                     /locus_tag="VLS86_03850"
     CDS             224..988
                     /locus_tag="VLS86_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO49656.1"
                     /translation="MRPDSSLPLLSVRSGTKRFGGVLALDEMDVDVFAGEVLAILGDN
                     GAGKSTLIKCMSGVHRLDTGVIEVDGDPVQLHSPTDARALGIETVYQDLALFDNLSPS
                     ANFYAGRELAGPRWLPMGMRLMRRRTMSQATQDVLDRLQVTLPDLEAPVGLMSGGQRQ
                     AVAVARATAFASRLVILDEPTAALGVRESARVLDLIRRLRDEGKAVVVISHAMDHVME
                     VADRAVVMRRGRKVGELVPSESSRERMVSLIVGGEG"
     gene            1003..>1575
                     /locus_tag="VLS86_03855"
     CDS             1003..>1575
                     /locus_tag="VLS86_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSO49657.1"
                     /translation="MVSGAELGGGVGCKKGLHPTDMRRIGMNSRTVRRWAAILVGMML
                     VAACGGDAGSTTTGPASTEPTTTDAPATTAAPATTTAGETYKLGLITKFPVDFFFIIE
                     DAAKAWAADHPDVELITGMGESGSDDEGVIALIESMVAQGVDGIAITPTGPAVTPALD
                     AAVAAGVKIVLIDNDLPDWDGKSAVVATDHL"
BASE COUNT          295 a          458 c          525 g          297 t
ORIGIN      
        1 tgatcatcgg cggcgcgagc tttctgggtg ggcggggaaa ggtgagtcac gccctggtcg
       61 gggcgttgat gatcggcgtg atcagaaact ccatgaacct ggccaacctg gattcgttct
      121 tccagctgat cgtccttggg atcgtgatca tcgtggccgt cgagtctgac gtgttgcgcg
      181 gccatctgga aaacaggctt cgtctgcgac aggcgaccag aacatgagac cggacagttc
      241 tcttccccta ttgagcgtgc ggagtggaac caagcgcttc ggtggggtac tcgccctcga
      301 cgagatggac gtcgatgtct tcgccgggga ggtgctggcg attctggggg acaatggtgc
      361 cggcaaatcg accctgatca agtgcatgag cggagtccat cgcctcgaca caggagtgat
      421 cgaggtcgac ggcgacccgg tgcagctgca cagccctacc gacgctcgcg ccctcggcat
      481 cgagaccgtc tatcaggacc tggcgctctt cgacaacctg agcccttctg ccaacttcta
      541 cgccggccgg gagctggccg ggccgcgctg gctgccgatg gggatgcggc tgatgcggag
      601 acggaccatg tcgcaggcga cacaagatgt cctcgaccgc ctccaggtca ccctgcctga
      661 cctcgaggca ccggttggat tgatgtccgg agggcagcgt caagcggtgg ctgttgcccg
      721 ggctaccgct ttcgcctcac ggcttgtgat cctggacgag ccgacagctg ctctcggagt
      781 acgtgaatcg gcacgggtgc tcgacctcat cagacgcctc agggatgaag ggaaagcggt
      841 ggtcgtcatc tcccatgcga tggaccacgt catggaggtg gccgatcggg ctgtggtgat
      901 gcgccgcgga cgcaaggtgg gcgagttggt tccttcagaa agctctcgag agcgaatggt
      961 ctctctgatc gttggaggtg agggttgacg aattgacttg gcttggtttc cggtgccgaa
     1021 ctgggtggcg gcgtcgggtg caaaaaagga ctccacccaa cagacatgag gaggatcgga
     1081 atgaattcaa gaacggtacg ccggtgggcg gcaattttgg tgggcatgat gcttgtagcc
     1141 gcctgcggcg gcgacgcggg ctcaactacc actggacctg ccagcacgga acccacgacg
     1201 accgacgcgc cggctactac cgccgcgccg gctacgacca ccgcaggcga gacctacaag
     1261 ctcgggctga tcactaagtt ccccgtcgac tttttcttca tcatcgaaga tgcagcaaaa
     1321 gcctgggcgg ctgaccatcc cgatgtcgag cttatcaccg gcatgggtga gtcagggtcg
     1381 gacgacgaag gtgtgatagc gctcatcgag tcgatggtgg cgcagggggt cgatggcatc
     1441 gccatcacac ccaccgggcc ggcagtgacc cctgccctcg acgcagcggt tgcggccgga
     1501 gtcaagatcg ttctgatcga caacgacctg ccggactggg atggtaagtc agccgtcgtg
     1561 gcgaccgacc acctc
//
LOCUS       DATITW010000272         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_293774, whole genome shotgun sequence.
ACCESSION   DATITW010000272 DATITW010000000
VERSION     DATITW010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293774"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VLS86_03860"
     CDS             complement(<1..480)
                     /locus_tag="VLS86_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HSO49658.1"
                     /translation="MARIALDAMGGDNAPTETVAGAVEASRRGVDVVLVGRYDVLQAE
                     LATHDRRLPIVDAPDVIEMGEDPARALREKPGASITVAARLVAEKSADGMVSAGSTGA
                     AMAAAAIIIGRIKGVARPSSASIFPTPGSPTLGLDSGANPDVKADQLVQFAIMGSVAA
                     "
     gene            complement(484..660)
                     /gene="rpmF"
                     /locus_tag="VLS86_03865"
     CDS             complement(484..660)
                     /gene="rpmF"
                     /locus_tag="VLS86_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HSO49659.1"
                     /translation="MAVPKKKMSRSRTRQRKAAWKVSRPSAVACEQCGKPKLPHRACR
                     ECGTYGGREVIEAR"
     gene            complement(676..1167)
                     /locus_tag="VLS86_03870"
     CDS             complement(676..1167)
                     /locus_tag="VLS86_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HSO49660.1"
                     /translation="MSPELTIPVGDLIGSPEKARPFSGTRSVSLRLGDTTVKGPMEVS
                     GRVIGVIDAVKAELTASAVAHLECTRCLAEWDETISVDVEQYFRVVPDEDGYAIVDNH
                     VDVSGPAQDELALALPAVPLCRPDCLGLCPTCGTDLNSDPCGGHGEDSESPFAVLRDL
                     FES"
     gene            complement(1170..1712)
                     /locus_tag="VLS86_03875"
     CDS             complement(1170..1712)
                     /locus_tag="VLS86_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49661.1"
                     /translation="MTQENESTEQVAMEPDEPEHRADLSDLQDTVDDLIVHLHEAKNV
                     PLSGNAMVDRELFLSKLEKVRADLPDELRAARWMVREREAFIARTNEKARDIVDRARK
                     EADEMVSDSSIVAEAVEEANILVRRAEGEARLLRLETEDEIEKNLQRVESILNEVMDQ
                     IQAARDELHQARPKPPDVPI"
     gene            complement(1709..2176)
                     /gene="coaD"
                     /locus_tag="VLS86_03880"
     CDS             complement(1709..2176)
                     /gene="coaD"
                     /locus_tag="VLS86_03880"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017837001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HSO49662.1"
                     /translation="MTTALCPGSFDPPTNGHVDVIERALSAFDDVVIAVVDNPSKASL
                     FSTEERVSLLQELFGDRAKIGSFSGLLVEYAAEVGANVVVKGLRTVDDYEYEMQMAQM
                     NRHLTGMETVFMATRSEWSFISSSLVKEVARLGGSITGLVPSVVEDALQERLG"
     gene            complement(2173..2721)
                     /gene="rsmD"
                     /locus_tag="VLS86_03885"
     CDS             complement(2173..2721)
                     /gene="rsmD"
                     /locus_tag="VLS86_03885"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HSO49663.1"
                     /translation="MRIITGKAKGRRLIGPDTPETRPVTDRVREAVFSIVGAWVEEAV
                     VLDLYAGSGSFGLEALSRGASTVTFVENGHRALESLRKNIATVGLGGTVVTTRVQEFL
                     SRSAGVFDLVFIDPPWTVPSPELTQDLYALDPLLGPQAEVILSRRHGDETPDSPESWR
                     VATDRRYGDTRIVRYEKEADLT"
     gene            complement(2725..>3096)
                     /locus_tag="VLS86_03890"
     CDS             complement(2725..>3096)
                     /locus_tag="VLS86_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HSO49664.1"
                     /translation="SQLHQLRGRVGRGSHQGTCILIADPSTPESEERLAAVEATNDGF
                     RLAQDDLRIRGQGTVFGARQSGMADLKLADVLGDFDLLVAARKDAFAVVDRDPDLSEH
                     PELAEEVRAMLGESVEWLFRS"
BASE COUNT          506 a         1069 c          962 g          559 t
ORIGIN      
        1 ggcggcgacc gaccccatga tcgcgaactg caccaactga tctgccttca catcggggtt
       61 ggcccctgag tcgagaccca aggtggggct gcccggggtt gggaagatcg aagcgctcga
      121 tggtcgggcg actcccttga tccgcccgat gatgatcgct gcggccgcca tcgcagcacc
      181 ggtagacccc gcggagacca tgccatcggc tgatttctcg gcgacaagcc tggcagcgac
      241 ggttatcgac gcgcccggtt tctcacggag ggcacgtgcc gggtcctcac ccatctcgat
      301 cacgtccggc gcgtcgacaa tagggagtcg tctgtcgtgc gtcgccaact cggcctggag
      361 aacgtcgtaa cggccgacca ggacgacgtc gacacctcga cgcgaggcct caaccgctcc
      421 ggcgaccgtc tcggtcgggg cgttgtcgcc ccccatagcg tcgagggcga tacgcgccaa
      481 gactcagcgg gcctcgatta cctcgcggcc gccgtacgtg ccgcactcgc gacatgcgcg
      541 atgcggcagc tttggcttgc cgcactgctc gcaagcgacg gcgctcgggc gggacacctt
      601 ccaggcggcc ttccgctgcc gggtccgcga gcgcgacatt ttcttctttg gcaccgccat
      661 tggtgctctc cttggtcagg attcgaacag atctctcagg acggcgaaag gtgactcaga
      721 gtcctctcca tgaccgccac aggggtcact attcaagtcg gttccacagg ttggacaaag
      781 tcccaggcaa tcgggccggc acagcggcac cgcgggaagg gccaaagcaa gctcgtcctg
      841 ggccgggccc gagacgtcta cgtggttgtc gacgatcgcg tagccgtctt cgtcggggac
      901 gacccgaaag tactgctcga cgtcgaccga gatcgtctcg tcccactcgg ccaggcagcg
      961 ggtgcactcg aggtgggcca ctgcgctggc ggtgagctcg gccttcaccg catcgatcac
     1021 cccgatgacc cggcccgaca cctccatcgg ccccttgacc gtggtgtcgc cgagccggag
     1081 actcaccgaa cgggtcccgg agaaaggtct cgccttctcc ggcgatccga tcaggtcacc
     1141 aaccgggatc gtcagctccg ggctcatgct cagatcggga cgtcgggggg cttgggccga
     1201 gcctgatgga gctcgtcgcg ggcggcctga atctggtcca tcacctcgtt gaggatggac
     1261 tcgacccgct gcaggttctt ctcgatctcg tcttcggtct cgagccggag caacctggcc
     1321 tcaccttctg cccttctcac caggatgttc gcctcctcga ccgcctcggc gacgatgctg
     1381 gagtccgaca ccatctcgtc cgcctccttg cgagcacgat cgacgatgtc ccgcgccttc
     1441 tcgttggtcc gggcgatgaa ggcctcccgc tcacgcacca tccagcgggc ggcccgcaac
     1501 tcgtcgggca ggtcggcgcg gaccttctcc agcttgctga gaaacaactc gcgatcgacc
     1561 atcgcattgc cggaaagggg gacgttcttg gcttcgtgga gatggacgat caggtcatcg
     1621 accgtgtcct ggaggtcgct gagatcggcc cggtgctccg gctcgtccgg ctccatcgcg
     1681 acctgctcgg tgctctcgtt ctcttgggtc atcccaaccg ctcctgaagg gcgtcctcga
     1741 cgacacttgg taccaggccg gtgatcgatc cgcccagcct ggcgacctct ttcaccagcg
     1801 aggacgagat gaagctccac tccgacctgg ttgccatgaa gactgtctcc atccccgtca
     1861 gatgccgatt catctgcgcc atctgcatct cgtactcgta gtcgtcgacg gtgcggagcc
     1921 ctttgaccac gacgttggcg ccgacctcgg ccgcgtattc caccagcagc ccggagaacg
     1981 acccgatctt tgcccggtcg ccgaacagtt cctgaagcag ggatacccgt tcctcggtgg
     2041 agaacagcga tgccttcgag gggttgtcga ccacggcgat cacgacgtcg tcaaacgcag
     2101 agagcgccct ttcgatgacg tcgacatggc cgttggtcgg cgggtcgaag ctccccgggc
     2161 agagtgcggt ggtcatgtga ggtctgcttc cttctcgtac ctgacgattc tggtatcccc
     2221 gtaccgtcta tcggtggcaa cccgccagga ttcaggtgaa tcgggggtct cgtcgccgtg
     2281 gcgacgggac aggatcacct ccgcctgagg gccgagcaac gggtcgagcg catagaggtc
     2341 ctgggtcagc tcaggcgatg gaaccgtcca cggcgggtcg atgaacacca gatcgaagac
     2401 cccggcggac cggctcagga actcctgcac cctggtggtc accaccgtcc caccgagacc
     2461 gaccgtggcg atgttcttcc tcagcgactc gagggcgcga tggccgttct cgacgaaggt
     2521 cacggtgctc gccccccgcg acagggcttc gagtccgaag gagccggatc cggcgtacag
     2581 gtcgagcacc accgcctcct ccacccaggc accgacgatg gaaaagaccg cctcccggac
     2641 ccggtcggtc accgggcggg tctcaggggt gtcgggcccg atcagtcgtc gtcccttcgc
     2701 cttgccggtg atgatcctca tcgctcagct ccggaacagc cactcgaccg actccccgag
     2761 catcgccctc acctcctctg ccagctcggg atgctcgctg aggtcgggat cgcggtccac
     2821 gacggcaaag gcatctttcc tggctgcgac caggaggtcg aagtccccga ggacgtcggc
     2881 cagcttgagg tcggccattc ccgactggcg ggccccgaac accgtcccct ggcccctgat
     2941 cctcaggtcg tcctgagcca gccggaaccc gtcgttggtg gcctccaccg cggcgagacg
     3001 ctcctcgctc tccggagtcg aagggtcggc gatgagaata caagtgccct gatggctccc
     3061 ccggcccacg cgccccctga gctggtggag ttggct
//
LOCUS       DATITW010000273         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_774497, whole genome shotgun sequence.
ACCESSION   DATITW010000273 DATITW010000000
VERSION     DATITW010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774497"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VLS86_03895"
     CDS             complement(<1..259)
                     /locus_tag="VLS86_03895"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HSO49665.1"
                     /translation="MSDGDLSSFESSIDEVFRRLGLPDPVLMSRLTADWEELAGSPWS
                     GHSTPLFIQGKTLVVEADSPSMVAFLRYGSADLIRVLGDLLG"
     gene            complement(256..1353)
                     /locus_tag="VLS86_03900"
     CDS             complement(256..1353)
                     /locus_tag="VLS86_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HSO49666.1"
                     /translation="MKVDWVALSEFRSYQTLEWNPDPGVNLLLGPNGAGKTNLLEAIA
                     YLASLRSFRSVPDVSLIRDDAEAAVIRAGISNQDRERLIEIEVQRVGPRRTQVDKSRL
                     HRTSDLLGALRVIAFLPEDLDLVKRGPAYRRDLLDAVAVQLWPAAYLDQSEFDRSLRQ
                     RNAFLRAGARDDATLSVWDSRLAQSGGRVMARRARVIETLSPLLDTSYEEIAQKRHEA
                     AIAYVASWGGADQILDRTAADFSAAILEAMETSRRTDYERRMTTVGPHRDEPVLRIDG
                     TDARTHGSQGEQRTMALAIKLASHRAVADLIGESPVLLLDDVFSELDPDRAAALAKAL
                     PGDTQTLISSARPEDVPVVGSVWKVGEGVHR"
     gene            complement(1354..2436)
                     /gene="dnaN"
                     /locus_tag="VLS86_03905"
     CDS             complement(1354..2436)
                     /gene="dnaN"
                     /locus_tag="VLS86_03905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HSO49667.1"
                     /translation="MRIRAQRDDLADVLARANRAVGARTALPVLQGLLCEVAGTTLRV
                     TGTDLDVTVRTAAEVEVMEEGRAVIPGRLLSEAVRKMPAGSVTIGVSDTDIEIQGNGP
                     RFTLRPLIVDDFPVQDDLVSDGVEVDGEDLAEAIAQVTTAASGDGARPILTGVLFEGS
                     EAGLRMVATDSYRLAKRDLKGVGLEGTGLVPARGLRELPRAIGAAKVTAQLREREAVF
                     TSDKGTLRLRLIDGNFPKYQSLLPDTYPNQVVIKKDVLLDALGRVTLVAEDHIPVRLK
                     LLEGGVEVSVTRQDVGGETEHIPGVFSGSDEEVSIAFNPRYLQDGVTVIPGDSIRIRV
                     IDAFKPSVIDSGAEDDFLYLLMPVRV"
     gene            complement(2609..>3203)
                     /locus_tag="VLS86_03910"
     CDS             complement(2609..>3203)
                     /locus_tag="VLS86_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSO49668.1"
                     /translation="EDRLRSRFEWGLLTDIQPPDVETRLAILRRNAEFAPRPVPLEVL
                     EHIAGLVSDNIRELEGALTRVTAWSALNRRDIDLETAERVLRDIVTTDEPVPLGPEII
                     MRTTASAYGFSVEDVLSPSRRQPLVLCRQISMYLCRELTDLSLPKIGEAFQRDHTTVL
                     HSVDKVKRILRSDRAVFDRVAQLTQDLRKGVGISTGV"
BASE COUNT          543 a         1029 c         1084 g          547 t
ORIGIN      
        1 cccccaacag gtcgccgaga acccggatca ggtccgcgga tccatagcgg agaaaggcga
       61 ccatcgacgg cgaatcggcc tcgacgacca gggtcttgcc ctggatgaag aggggtgtcg
      121 aatgacccga ccacggggag ccggcgagct cctcccagtc ggcggtgagc cgggacatca
      181 gcaccgggtc gggcagcccg agccgtcgga agacctcgtc gatggacgac tcgaacgacg
      241 agaggtcgcc gtcgctcacc ggtggacccc ctcccccacc ttccacaccg agccgacgac
      301 gggcacgtcc tcggggcgcg ccgagctgat caaggtctgg gtgtcaccgg gcagcgcctt
      361 ggccagggcg gcggcccgat ccgggtcgag ctcggagaac acatcgtcga ggaggagcac
      421 cggcgactcg ccgatcaggt cggcgacggc gcgatgtgag gccagcttga tcgccagggc
      481 catggtccgt tgctcgccct ggctgccgtg ggtgcgggcg tcggtgccgt cgatgcggag
      541 caccggctcg tcacgatgag gcccaacggt ggtcatcctc cgttcgtagt cggtccggcg
      601 tgatgtctcc atcgcctcca ggatcgcagc tgagaagtcg gcggcggtgc gatcgaggat
      661 ctggtcggca ccaccccacg acgccacgta ggcgatcgcc gcctcgtgtc gcttctgggc
      721 gatctcctcg taggacgtgt cgagcagggg ggaaagggtc tcgatgacac gagcccgtcg
      781 cgccatcacc cgcccccctg attgggcgag ccgggagtcc caaaccgaca gggtcgcgtc
      841 gtccctggct cctgcccgga gaaaggcgtt cctctggcgg agagagcgat cgaactccga
      901 ctggtcgaga taggcagccg gccaaagctg gaccgccact gcgtcgagga ggtcgcgccg
      961 gtaggccggc cccctcttca ccagatcgag gtcctcgggg aggaacgcga tcacacgcag
     1021 cgcgcccagc aagtcggagg tccggtgaag acgtgacttg tcgacctggg tccgccgcgg
     1081 cccaacccgc tgcacctcga tctcgatcag gcgctctcga tcctgattgg agatcccggc
     1141 ccttatcacc gccgcctcgg cgtcgtccct gatcagggag acgtcaggga cggagcgaaa
     1201 cgagcgcagg gacgccagat aggcgatggc ctcgagcaga ttcgtcttcc cggcgccgtt
     1261 ggggccgagg aggaggttga ccccgggatc agggttccac tccagagtct gataggagcg
     1321 gaactcggag agggcgaccc agtcgacctt cattcaaacc cggacgggca tgagcagata
     1381 gaggaagtcg tcctcggctc cactgtcgat cacgctcggc ttgaacgcgt cgatcaccct
     1441 tatccgaatc gagtcgcccg ggatcaccgt gaccccatcc tggaggtatc tcgggttgaa
     1501 ggcgatcgag acctcttcgt cggacccgga gaagaccccc gggatgtgct cggtctcgcc
     1561 acccacgtcc tgccgggtga cgctgacctc gaccccgccc tccaacagct tgagccggac
     1621 cgggatgtga tcctcggcaa ccagcgtgac acgacccagc gcgtcgagca ggacgtcctt
     1681 cttgatcacc acctgattcg ggtaggtgtc cgggagcagg gactggtact tggggaagtt
     1741 cccgtcgatc aaccggaggc ggagcgtgcc cttgtcggag gtgaacaccg cctcgcgctc
     1801 cctgagctgg gcggtgacct tggccgcccc gatggcccga ggtagctccc gcagcccccg
     1861 ggccgggacc agccccgtac cttctaggcc aacccctttc aggtcgcgct tagccaggcg
     1921 ataggagtcg gtggcgacca tcctcagccc ggcctcggag ccttcgaaca gtacaccggt
     1981 cagaatcggc cgggccccgt ctcctgacgc cgcggtcgtg acctgggcaa tggcctcggc
     2041 caggtcctca ccgtcgacct ccaccccgtc ggacaccagg tcgtcctgaa ccgggaagtc
     2101 gtcgacgatc agtgggcgta gggtgaagcg cggcccgttg ccctggatct cgatgtcggt
     2161 gtcgctgacc ccgatggtca ccgagccggc cggcatcttc cgcaccgcct ccgagagcag
     2221 ccgacctggg atcacggccc tgccctcctc catgacttcg acctcggccg cggttcgaac
     2281 cgtcacatcg agatcggtgc cggtgacgcg aagcgtggtc cccgccacct cacagagcag
     2341 accctggagg acgggaaggg cggtgcgagc cccaactgcc cgattggctc gtgcgaggac
     2401 gtcggcgaga tcgtctcgct gggctcggat tctcacaggt ggtgatccct tctgcttctg
     2461 ttcttgtatc tcaattaaag agtattagtc gtagtagagg gttgtggata tggggaaagc
     2521 cccgatatct ctatgtggtg gttgatgatt cggctgtgca tggtgttgtt ggtggtgatg
     2581 gcacaacagg gcggtgtctg aggacaactc aaacccctgt cgatatcccc acgcctttcc
     2641 tcaggtcctg tgtgagttgt gccacccggt cgaacacggc gcgatcggag cgaaggatgc
     2701 gcttcacctt gtccactgaa tggaggacag tggtgtggtc gcgttggaat gcctcaccga
     2761 tcttgggcag tgacaggtcg gttagctcgc ggcacaggta catcgagatc tggcggcaca
     2821 ggaccagcgg ctgacgccgg ctcgggctga ggacgtcttc cacggagaag ccgtaggccg
     2881 aggccgtggt gcgcatgatg atctccgggc ccagcggcac cggctcatcg gtggtgacga
     2941 tgtccctcag cacacgctcg gctgtctcca ggtcgatgtc gcggcggttg agggcggacc
     3001 aggcggtgac tcgggtcagg gctccttcga gctcgcggat gttgtccgag accagaccgg
     3061 cgatgtgctc gaggacctcg agcggcacgg ggcggggggc gaactcggcg ttcctcctga
     3121 ggatggcaag gcgggtctcg acgtcgggtg gctggatgtc ggtgaggaga ccccattcga
     3181 accggctgcg gagccggtct tcg
//
LOCUS       DATITW010000274         2690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_453949, whole genome shotgun sequence.
ACCESSION   DATITW010000274 DATITW010000000
VERSION     DATITW010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453949"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..987)
                     /locus_tag="VLS86_03915"
     CDS             complement(40..987)
                     /locus_tag="VLS86_03915"
                     /inference="COORDINATES: protein motif:HMM:NF044626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49669.1"
                     /translation="MGEALTQQELEAAHCWEAADRVRRPEMTEFRRRLRYRQAQWREA
                     KGHPIGTQPMVPKPGGPMRLVGSRVPLDYARETGANFLTPTALEAARERTSIKEREQS
                     FDRQGLWADLLSSAAMSFNLFGDLAADIGLADLAVHTWWPDVPGRVSEVRFAHSPGCL
                     DLAYIGNLSAFDVAFVLDLDDGTKGILGLITKYHEWTKPETPKPENLWRYLEVATRSG
                     SFLAGATDGVSGRSDLWEPWLTHLLLLSMLQHPEGTWSWGRCVFVHPADNSDFADACA
                     RYRGLLADQSTFSSLTIEELLAAEVLPAATAAAFRERYL"
     gene            1107..1625
                     /locus_tag="VLS86_03920"
     CDS             1107..1625
                     /locus_tag="VLS86_03920"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSO49670.1"
                     /translation="MGVGTLRCVVINVTDLRLAYEFWGAVTGLEVIGSETGWNDWLGY
                     LGTKDPRKHEIILQRVDGSPLEIETPNPDKPNQVHIDITPNAGIDQAINDIVALGGRV
                     KKAPSLYPRPGSHGDQQPVIDWAVMQDPFGNEFCLVDDLTLDQSHAAMTSVDATTDSE
                     WRIAAGVAREGD"
     gene            1872..2255
                     /locus_tag="VLS86_03925"
     CDS             1872..2255
                     /locus_tag="VLS86_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49671.1"
                     /translation="MEQAAILAALLMCVVAVFHIALALGAPAGYAAWGGWHEGVLPTR
                     LRIASGVVGIGVYPALIVFVLASAGVIDANWVPGTGRLGMWILTGLFTLGTLANFASR
                     SKRERWWGFVSLAIAICCAVVALSL"
     gene            complement(2310..2384)
                     /locus_tag="VLS86_03930"
     tRNA            complement(2310..2384)
                     /locus_tag="VLS86_03930"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2347..2349),aa:Ile,seq:cat)
     gene            complement(2421..2684)
                     /locus_tag="VLS86_03935"
     CDS             complement(2421..2684)
                     /locus_tag="VLS86_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSO49672.1"
                     /translation="MAGVVGSRRFAYDLWGDTVNTASRMESQGHAGQIQVTEAVRRSA
                     AGRFDFSPAGVIAVKGKGPMETFLLDGPATEGTVNHDAGNEKR"
BASE COUNT          506 a          850 c          850 g          484 t
ORIGIN      
        1 ggggggttct ctaatcgtct gacagtcctt tcagaccggt cacaagtacc gctcgcgaaa
       61 cgctgccgcg gtcgccgccg gaaggacttc ggcagcgaga agctcttcga tggtcaaaga
      121 ggagaaggtc gactggtcgg cgaggagccc tcgatagcgg gcgcaggcgt cggcgaagtc
      181 cgagttgtcg gcgggatgga cgaacacgca tcgcccccag ctccaggtgc cctcgggatg
      241 ttggagcatc gacaagagca gcaagtgggt gagccagggc tcccagagat ccgacctccc
      301 actgacacca tcggtcgccc ctgccaggaa gctgcccgac cgcgtggcga cctccaggta
      361 acgccagagg ttctccggtt tcggtgtctc cggcttggtc cactcgtggt acttggtgat
      421 gaggccgagg atccccttcg tcccgtcgtc gaggtcgagc acgaaagcca cgtcgaaggc
      481 gctcaggttg ccgatgtagg cgagatcgag acaccccggc gagtgggcga acctgacctc
      541 ggacacgcgg ccggggacgt cgggccacca ggtgtgaact gcgaggtcgg cgagcccgat
      601 gtcggcagca agatcaccga acaggttgaa ggacatcgct gccgaggaga gaaggtcggc
      661 ccacagaccc tggcgatcga agctctgttc cctctccttg atcgaggttc gctccctcgc
      721 cgcctcgagg gcggtgggag tgaggaagtt ggcgccggtc tcccgggcgt agtcgagggg
      781 aacacgactc cccaccagcc gcatcggtcc cccgggcttg ggaaccatcg gctgggtacc
      841 gatgggatga cctttggctt cccgccattg cgcctgccgg tagcgcagcc tccgacggaa
      901 ctcggtcatc tccggtcgcc gcacccggtc ggcagcctcc caacaatggg ctgcctcgag
      961 ctcctgctgg gtcaaagcct cacccaccgg ctcaatggta gggatggcat cggtcacgta
     1021 gcctcggagg aaaccagaac gccccatcaa gggcaggggc ctcagaatgg cggtcatgag
     1081 cggggcggat cagagaggag ccacacatgg gcgtagggac acttcgctgc gttgtgatca
     1141 acgtcaccga ccttcggctc gcctacgaat tctggggcgc ggtcaccggt ctggaggtga
     1201 tcggatccga aaccggctgg aacgactggc tcggatatct aggcaccaag gacccacgga
     1261 aacacgagat catcctgcag cgtgtcgacg gctccccgct cgagatcgag acacccaacc
     1321 cggacaagcc caaccaggtg cacatcgaca tcaccccgaa tgccggcatc gaccaggcca
     1381 taaatgacat cgtggcactg gggggcagag tcaagaaggc accaagtctc tatccccgtc
     1441 ccgggtccca tggcgatcag caacccgtca tcgactgggc cgtcatgcag gaccctttcg
     1501 gcaacgagtt ctgcctggtc gacgatctga cactggacca atcccacgcg gccatgacct
     1561 ccgtcgacgc aacgacggac tcagagtggc ggatcgccgc cggagtggct cgggaaggcg
     1621 actgagccaa ggggactcaa aggttccagt gagatgtgaa gtgggcctaa cgggacagaa
     1681 cgcgaacctc atccacagct ggtggggcac aatacgtttc ctgggtcagc tccgatgagc
     1741 gaaccagtcg ctcgaggcgg ccgatcgtcg tgaaccgagg atgtaggggt acatctcacc
     1801 cttgcctttg acggagatga cgcctcgcgg ttcgcagatg aaccctctcg cggattagct
     1861 accgtccacg gatggagcaa gccgccatcc ttgccgcgct gctgatgtgc gtggtcgccg
     1921 tgttccatat tgccctcgct ctgggcgcac ccgctgggta cgccgcctgg ggcggctggc
     1981 acgagggtgt tctcccaacg cggttgcgta tcgccagtgg tgtcgtcgga atcggggtct
     2041 acccggcgct catcgtcttc gtcctggcta gcgccggagt cattgacgcc aactgggtcc
     2101 ctggaaccgg gagactgggg atgtggatcc tcaccggcct cttcaccttg ggcaccttgg
     2161 ccaacttcgc atcccgctcg aagcgggagc gttggtgggg attcgtgtcc ctcgccatcg
     2221 cgatctgctg tgcggtggtc gccctgtcgc tctgatgaat caccccagac gggtggtccg
     2281 ggctcttatg gccaggactc atcattttgt gggcccggca ggacttgaac ctgcaaccaa
     2341 cggattatga gtccgctgct ctgaccggat tgagctacgg gcccgaggaa tccaattgtg
     2401 gcagtcgcat gctggttagc tcatctcttc tcgttccccg catcatggtt gacagtgcct
     2461 tcggtcgcag gaccgtccaa tagaaacgtc tccatgggcc cttttccctt gacggcgatg
     2521 acgcctgccg gagagaagtc gaaacgcccc gcagccgagc gacgtacagc ctcggtgacc
     2581 tgaatttggc cggcatggcc ttgactctcc atccgactag ctgtgttcac cgtgtcgccc
     2641 caaaggtcat atgcaaaccg ccgagatccg accactccgg ccacgaccgg
//
LOCUS       DATITW010000275         1741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_293800, whole genome shotgun sequence.
ACCESSION   DATITW010000275 DATITW010000000
VERSION     DATITW010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293800"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VLS86_03940"
     CDS             <1..561
                     /locus_tag="VLS86_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HSO49673.1"
                     /translation="RDGFVMGEGGAALVLEELGHAQARGAKVLAEITGYGMSADAYHI
                     TLPRPGGSGAARAMVNAIADAGLTPPDIGYINAHGTSTEANDSTETAAIHIAMGESAQ
                     STPVSSTKSMTGHVLGGAGAVEAVACILALRDQVLPPTINYEVPDPDCDLDYVPNVKR
                     EASIDHAMSNSFGFGGHNVSLVFSRP"
     gene            complement(563..814)
                     /locus_tag="VLS86_03945"
     CDS             complement(563..814)
                     /locus_tag="VLS86_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49674.1"
                     /translation="MRRRPNPWIAVPALFLGALAAALGWIVTDVSCRVPVDGGGYTTC
                     VGWATLVAIVSFLAVTIGAAVILVLVYRSLAEWRESSGR"
     gene            complement(811..1557)
                     /locus_tag="VLS86_03950"
     CDS             complement(811..1557)
                     /locus_tag="VLS86_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49675.1"
                     /translation="MKVSLASATPLLAALLWVVALLVDPGPFAPWPVLLIGLGLLETA
                     CVSVVGMIMTGGRWARRTALAVVVATLGVAAARPLDALWVIALAATTLAAVSMFTGSV
                     IRSTRKLPAAFGPPERAVLIPLLLLGFPTLLGFAAWDEQMASTLAVGLTAPIAAIWYS
                     RVLPGGLLAIRILWPTLAIGLAAFQGPAPGVTSLVVGALVAVLAWHPSVKVAFHPPRE
                     KGTTYAIPPELAPREVLDAARLDEKGRPRG"
BASE COUNT          316 a          600 c          595 g          230 t
ORIGIN      
        1 cgcgacggct tcgtcatggg cgagggggga gcggcgctgg tcctcgagga gctgggccat
       61 gcccaagcac gaggggcgaa ggtgctcgcg gagatcaccg ggtacgggat gtctgctgac
      121 gcctaccaca tcaccctgcc caggcccggt ggctcgggtg ccgccagggc catggtcaac
      181 gcaattgccg acgccggtct caccccaccc gacatcgggt atatcaacgc ccacggcacg
      241 tcgaccgagg ccaacgactc caccgagact gcggcgatcc acatcgcgat gggcgagtcg
      301 gcgcagtcga caccggtctc gtcgaccaag tcgatgaccg gtcacgtcct gggtggcgcc
      361 ggagccgtgg aggcggtggc gtgcatcctg gcgctcaggg accaggtcct gccaccaacc
      421 atcaactacg aggttccgga cccggattgc gatctcgact atgtgcccaa cgtgaaacgg
      481 gaagcgtcga tcgatcacgc catgtcgaac tcgtttggct tcggcgggca caatgtgagc
      541 ctggtgttct ccagaccgtg agtcagcggc cgctgctctc ccgccactcg gccaggctcc
      601 ggtagaccag gacgaggatc acggcggcgc cgatcgtgac cgcgagaaac gacacgatgg
      661 cgaccagcgt cgcccacccg acacaggtcg tgtagccacc tccgtccacc ggcacccggc
      721 agctgacgtc ggtgacgatc catcccagcg cagcggccag cgcccccagg aacaaggccg
      781 ggacggcgat ccacggattg ggccggcgtc tcatccccgg ggccggcctt tctcgtcgag
      841 cctggctgca tcgagcacct cccggggggc gagctcgggg ggtatcgcat aggtagtccc
      901 cttctcccgg ggcgggtgaa aggcgacctt gaccgatggg tgccaggcca gcacggcgac
      961 cagggcgcca acgaccaggg aggtgacgcc cggtgccggg ccctggaacg cagcgaggcc
     1021 gatcgccagg gtgggccaca ggattcggat ggcgaggaga ccacccggca gcactcgcga
     1081 gtaccagatc gcggcgatcg gcgcggtcag gccaacggcc agcgtgctcg ccatctgctc
     1141 gtcccaggcg gcgaacccga ggagggtggg gaagccgagc aggagcagcg ggatgagaac
     1201 cgcccgctcc ggggggccaa aggcggcagg cagcttccgg gtgctacgaa tcaccgatcc
     1261 ggtgaacatc gagaccgcgg ccaatgtggt cgcggccagc gctatcaccc acaacgcgtc
     1321 gagtgggcgg gcagcggcca ctcccagtgt cgccaccacc acggccagag ccgtcctcct
     1381 cgcccaccgc ccaccggtca tgatcatccc gaccaccgag acgcaggccg tctcgagcag
     1441 ccccaacccg atgagaagca ccggccacgg tgcaaacggg cccgggtcga cgagcagggc
     1501 gacaacccag agcaatgctg cgagaagagg ggtggcggag gcgagcgaga ccttcacggt
     1561 cggattgtcg cagcggccct tctcaagccg aagtcgaatc ctccggctcc ggtgaggggt
     1621 ggaggaccct gagacctgac ttctcgccga agcgggggga ttcccctggc tcccacaccg
     1681 tctccaatcg ccacgcggtc cccggtctga gggtgaccac ggcgccgtgc cctggcttga
     1741 c
//
LOCUS       DATITW010000276         1519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_614432, whole genome shotgun sequence.
ACCESSION   DATITW010000276 DATITW010000000
VERSION     DATITW010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614432"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1519)
                     /locus_tag="VLS86_03955"
     CDS             complement(<1..>1519)
                     /locus_tag="VLS86_03955"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HSO49676.1"
                     /translation="IPTLRDAPADAEAIGHKLLVRGGYIRQLAAGSYSILPLGVRVRT
                     KVAGIIREEMNRIGGQEFLLPALHPAEIWKTSGRWTLMGDNLFRLQDRKQADHALGMT
                     HEEVFAGIATELFSYRELPQIWYQIQTKFRDEPRPKSGLLRVREFTMKDSYTLDIDRP
                     GLDSGFDKHRAAYFSIFQRCGLDPVDVEASSGAMGGSESVEFMVRSEAGEDWIVTCAH
                     CGYRANVERAASAVAPVEDGESSNLERFPTPGLRTIKVLAAAHPEIASPDRQIKTLVY
                     ILDGEVVLVLLRGDHDLQEQKLMDATGAIEIRPSLPEEIRELLGAEPGSLGAVEVKGP
                     TVIADLALKDRKNMVTGANEDDWHHSGGDLERDIGVGIWADLRAVGDAEPCSVCGNPL
                     DRWKGIEVGHIFKLGTKYSEAFGATVQDESGASWPIIMGSYGIGVERAMAAVVESSHD
                     DGGIIWPVSVAPYEVVITVVRPDDRPTMEAANRVYQELAEAGIEVLLDDRDERPGV"
BASE COUNT          257 a          516 c          471 g          275 t
ORIGIN      
        1 tgacgccggg acgctcgtct cggtcgtcga ggagcacttc gatacccgct tcggccaact
       61 cctgatagac ccggttggcg gcctccatcg tcgggcggtc gtcgggacgg accaccgtga
      121 tcacgacctc atagggggct acggacaccg gccagatgat cccaccgtcg tcatgtgagg
      181 actcgaccac cgccgccatc gcccgttcga caccgatccc gtaagagccc atgatgatcg
      241 gccagctcgc accagattcg tcctgaaccg ttgcgccaaa ggcctccgag tacttggtcc
      301 ccagcttgaa gatgtgaccg acttcgatcc ccttccagcg gtccaatggg ttgccacaga
      361 ccgagcaggg ctcggcgtcg cccaccgctc gcaggtcggc ccagattccg acgccgatgt
      421 cgcgctcgag gtcgccgccc gagtggtgcc agtcatcctc gttggcgccc gtgaccatgt
      481 tcttgcggtc cttgagggcg agatccgcga tcacggtcgg ccctttgacc tccaccgctc
      541 ccagactccc gggctcggcg ccgagcagct cccggatctc ctcgggcagc gagggccgga
      601 tctcgatcgc accggtggcg tccatcagct tctgctcctg gaggtcgtga tccccccgca
      661 gcaacaccag gaccacctcg ccgtcgagga tatagaccag agtcttgatc tgacggtccg
      721 gcgacgcgat ctcagggtgg gccgcggcca ggaccttgat ggttctcaga cccggcgtcg
      781 ggaaccgctc cagattcgag ctttccccat cctcgacggg ggcaacggcc gacgccgccc
      841 gctccacatt cgctcgatag ccacagtggg cgcaggtgac gatccagtcc tcacccgctt
      901 cgctgcgcac catgaactcg accgactccg atccccccat ggctcccgac gacgcctcga
      961 cgtccaccgg gtcgaggcca catcgctgaa agatcgagaa gtatgcggcg cggtgcttgt
     1021 cgaagccgct gtcgaggccg ggtcggtcga tgtcgagtgt gtaggagtcc ttcatggtga
     1081 actcacgcac cctgaggaga ccggacttgg gacggggctc gtctcggaac ttggtttgga
     1141 tctggtacca gatctgggga agctcgcggt aggagaagag ctcggtggcg atgccggcaa
     1201 acacctcttc gtgggtcatg cccaaggcgt gatctgcctg cttgcggtcc tggagcctga
     1261 agaggttgtc ccccatcagg gtccaacgcc cgctcgtctt ccaaatctcg gcggggtgaa
     1321 gagcgggaag caggaactcc tgacctccga tccggttcat ctcctcccgg atgatcccgg
     1381 ccaccttggt ccggaccctg acccctagag ggaggatcga gtaggagccg gcggcaagct
     1441 gacgaatgta tccgccccgc accaggagct tgtgaccgat cgcctcggcg tccgccgggg
     1501 cgtctcgaag ggttgggat
//
LOCUS       DATITW010000277         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_734846, whole genome shotgun sequence.
ACCESSION   DATITW010000277 DATITW010000000
VERSION     DATITW010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734846"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VLS86_03960"
     CDS             <1..668
                     /locus_tag="VLS86_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSO49677.1"
                     /translation="AKVVLVSRNAGDLAEAAQELTAEGCDVVASPFDVSNIDCVEPWV
                     SDTWSDVGPIDILLHSAGHQRRGAAVDLDYADWEQILAVNLTGPFFLSRAVGKRQIEA
                     GSVGSHIFVGSLTTSIGIAYTAAYSASKSGVAGVVRTLAVEWAQSGIRVNAIAPGYFR
                     TQLTEGLFQDPDRSAWIQSRIPMGRIGVPDDLGGAAVFLASEASAYITGSILNVDGGW
                     LAG"
     gene            complement(683..2134)
                     /locus_tag="VLS86_03965"
     CDS             complement(683..2134)
                     /locus_tag="VLS86_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSO49678.1"
                     /translation="MSDPRIDYRIRMLLHELADVVGAKNVSVDPGELAAQAADWSWMS
                     QLYRLHGIELPTADVMLRPGSAQEVADVVRIASDHRVPVVPRGGGSGTQGGTFALYGG
                     IALDLTRMDRILEVDETSLVVKLEAGVTAPQLEEALSPLGLAVAHEPGSFHFGATVGG
                     WLAARGSGVRSTKYGKPEDMVLQVEAALPPGQLTSTPSVPNHAAGPGLLQLLIGSEGT
                     MGVITSATLRMDPLPESQAFLTYEFATVFDGLEAGRRIMTQRWRPSVMRLYDEADRDK
                     LNHILGLELTGALLVIVCDGDQRLVDLESEAITAICSEVGGKYLGPGGAKTWWDGKYE
                     PYAEGKAPAPPTIFGTTDTVCTFDNMPDLYRAKRKNVEEGFAEYGAQYTAHFSHWFPW
                     GVMVYDRFYIHDSPEDPMEALDLHDRLWDSAVRTSLAHGGVINEHHGVGVKLGRFMRE
                     QYGPMWPYLQGIKNAIDPDGILNPGKLGFGPPR"
     gene            complement(2127..2822)
                     /locus_tag="VLS86_03970"
     CDS             complement(2127..2822)
                     /locus_tag="VLS86_03970"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSO49679.1"
                     /translation="MAEPDPTTVTDRAYVQLRELLTSENVHPGDHLGEVAVAEWLGIS
                     RTPVRAALQRLELDQLVRRNHNSGYSVAGLTAGDVHEICDVLRLLDTALFIRAAENLT
                     DEQSTNLEECVERMAMAASKRDLELWSEGDARFHELLQDSSDHTLFSDIAEKQRRRLH
                     RFWMSGAGRLERLAKCADEHSSIAKSIAASDHDGVRRLVNEHIDHMEESMIRHLEMAR
                     PFISTNGSALDHV"
BASE COUNT          506 a          877 c          949 g          561 t
ORIGIN      
        1 gggcgaaggt ggtgctggtc agccgcaatg ccggcgacct ggctgaagcg gcccaggagc
       61 tgactgccga gggttgcgac gtggtcgcca gcccgttcga cgtgtcgaac atcgattgcg
      121 tcgaaccgtg ggtctccgat acctggtctg atgtgggacc gatcgacatc ttgcttcatt
      181 ccgccggcca tcaacgtcgg ggagctgccg tcgacctgga ctacgccgac tgggaacaga
      241 tcctggctgt gaatctgaca gggcccttct ttctttcccg ggcggttgga aagcgccaga
      301 tagaggccgg ttcggtcgga tcccacatct tcgtgggctc gctgacgacg agcatcggga
      361 tcgcctacac ggcggcctac tcggcctcta agagcggagt cgccggagtg gtccgaacct
      421 tggcggtcga atgggcacag tcgggtatcc gcgtcaacgc catcgctcct ggctatttcc
      481 gaacccagct caccgagggc cttttccagg atccagatcg aagtgcgtgg atccaatcgc
      541 ggatccccat gggccggatc ggagtcccgg acgacctggg cggggctgct gtgtttctcg
      601 cgtccgaggc gtccgcgtac atcacgggct ccatcctcaa cgtcgacggt ggctggctcg
      661 ccggttgacg tgtggcctgc cttcaccggg gtggtccgaa gccgagcttg ccgggattga
      721 ggatgccgtc aggatcgatg gcattcttga tgccttgcag atagggccac atggggccgt
      781 attgctcacg catgaagcga ccgagtttga ccccaacgcc gtgatgctca ttgatcacac
      841 cgccgtgcgc gagcgatgtg cgaaccgccg agtcccacaa tcggtcgtgg aggtcgagcg
      901 cctccatcgg gtcctcgggg gagtcatgaa tgtagaaacg gtcatagacc atcactcccc
      961 acgggaacca gtgggagaag tgtgcggtgt actgggctcc gtactcggcg aagccctcct
     1021 ccacgttctt gcgcttggcc cgatagaggt cgggcatgtt gtcgaaggtg caaaccgtgt
     1081 cggtggtgcc gaagatcgtc gggggagcgg gtgcctttcc ttcggcgtag ggctcgtatt
     1141 tgccatccca ccaggtcttg gcgccgccgg gaccgagata tttcccgcca acctcgctgc
     1201 agatcgctgt gatcgcctct gattcgaggt caacgagacg ctgatccccg tcacaaacaa
     1261 tcaccagcaa ggccccggtc agttcgagcc cgaggatgtg gttcagcttg tcccgatcgg
     1321 cttcgtcata aaggcgcatc accgaggggc gccaacgctg agtcatgatt cgtcgacccg
     1381 cttcgagccc atcgaagacc gtcgcaaact cgtaggtgag gaatgcctgg gactcgggca
     1441 atgggtccat gcggagagtg gccgaggtga tgacacccat ggtgccctcg gaaccgatta
     1501 ggagttgaag cagcccgggg ccggccgcat gattgggtac cgagggcgtc gacgtcagct
     1561 ggccgggggg aagggcagcc tcaacctgaa gaaccatgtc ttcgggtttg ccgtacttgg
     1621 tcgatcggac gccagaccct cgagcggcca accagccgcc gacggtggcg ccaaagtgga
     1681 atgagccagg ctcgtgcgcc acggcgaggc caagagggct gagtgcctcc tcgagttggg
     1741 gcgcggtcac gccggcctcg agtttgacga ccaagctggt ctcgtccact tcgaggatgc
     1801 ggtccatccg ggtcaggtcg agggcgattc ctccgtacaa ggcaaacgtc ccaccttgag
     1861 tgcccgaccc accgccacgc ggcacaaccg ggacgcgatg atcgctggcg attcggacca
     1921 catcggccac ctcttgagcg cttccgggtc ggagcatcac atcggccgta ggcaactcga
     1981 tgccgtggag tctgtagagc tgggacatcc acgaccaatc tgctgcctgg gcagccagtt
     2041 ccccggggtc gaccgagacg ttcttggcgc cgaccacatc cgcgagctca tggaggagca
     2101 tccggatccg gtagtcgatg cggggatcag acatggtcga gggctgatcc gttggtggat
     2161 atgaaggggc gcgccatctc gagatggcgg atcatgctct cttccatgtg gtcgatgtgc
     2221 tcgttgacga gtcgtcgaac gccgtcatga tccgaggcag cgatcgattt ggcaatggaa
     2281 gagtgctcgt cggcgcactt ggccaatcgt tcgaggcggc cggcccccga catccaaaat
     2341 cggtgaagcc gtcttcgctg tttctcggct atgtcggaga agagcgtgtg gtccgacgag
     2401 tcctggagga gctcgtggaa ccgggcgtcg ccttcggacc agagttcgag gtcacgtttc
     2461 gaggccgcca tcgccattct ctcgacgcac tcttcgaggt tggtgctctg ctcgtcggtg
     2521 aggttctccg cggctcggat gaagagggcg gtgtcgagga gacggaggac gtcgcagatc
     2581 tcgtggacgt ctccggccgt cagcccggcc accgagtagc cactgttgtg gttgcggcgc
     2641 acgagttgat caagttcgag tcgctgtaac gcggccctca ccggtgtgcg gctgatgccc
     2701 agccactcgg caacagccac ctcgcccagg tgatcgccgg gatggacgtt ctcgctcgtc
     2761 agcagctcgc gcagctggac gtacgccctg tcggtgacgg tcgtgggatc gggctctgcc
     2821 atcacaagac tcggtcgatg aactctcggt tctcgacctt gaccggaacc ttcatcagct
     2881 ccagcacccc ccg
//
LOCUS       DATITW010000278         4736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_173960, whole genome shotgun sequence.
ACCESSION   DATITW010000278 DATITW010000000
VERSION     DATITW010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173960"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3215)
                     /gene="gltB"
                     /locus_tag="VLS86_03975"
     CDS             complement(<1..3215)
                     /gene="gltB"
                     /locus_tag="VLS86_03975"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HSO49680.1"
                     /translation="MTPPFFDPGPDRDACGVGFIAALDRAPSYRMTRLAVECLERLDH
                     RGARAADGTGDGAGLLTQIPLRLITRELSAHGLVVSPEHLGVVMCFLSPTDAAASRSV
                     VTQSIEAEGMRVLFWRSVPIDPSVLGDHARLVLPLIEQAIVTGSGDSAGFERSLFLAR
                     KRIERAATPGLSIPSASSRTVVYKGLFTASHISNFYWDLRDPDYETAFAIFHQRYSTN
                     TFPSWDNAQPFRALAHNGEINTIQSNRSWMVAREKGATPGVWGDRLPDVFPFLQPGIS
                     DSGSLDNVFELLMRSGRSLTHVKEMLMPAAWENVPDLDPALASFYAYHAFLTEPWDGP
                     AAIAATDGTSLLVGMDRNGLRPARWAITPEVVLVASEAGVCPEEEARATATGQLGPGE
                     VIFFDGVSGEVQHSDQVKAALARARPYGDWISTETLRIQASFDGTADDRFDAAALSRV
                     FGYTAEERRLIIADMARGENPLGSMGDDTGLAVLSSRPRRLTQFFHEQFAQVTNPPMD
                     PIREKLVMSLRIQLGRRGPLLEDTPQQAHLIELASPILSEAELEAIVRSGDPRFFSHW
                     IATTWPVLSGPDGMEGRLDEICDEAAQAVRLGARIIVLSDRETDANRAAIPILLALGA
                     VHPHLIDEGVRGSVSLVVVSGEPRDPHDLACLIGFGASAVNPYLAIEHARDLAASGAI
                     DVDPVPAQENFRLAMESGLLKIMSKMGICTLSAYRGSELFEVIGLSEAVCRKAFRNAP
                     RRLRGIGLEELARQALQRHESYSGGSPDSGGFYKHRRGSDTHVTSPMVVLALQKSVRS
                     GDPEDWDRYIEMISDREPALVRDLLTFVQREPVPLDEVEPAEQIMRRFTTAAMSLGAL
                     SSEAHVALAEAMNMIGGHSNSGEGGEDPARFGTPKNSAIKQVASGRFGVTPAYLHSAD
                     ELQIKMAQGSKPGEGGQLPGHKVSDEIARLRYTEPGVTLISPPPHHDIYSIEDLAELI
                     FDLKAFKPTARVSVKLVSEPGVGTIAVGVAKADADVIVISGSEGGTGASPLVSIKHAG
                     SPWELGLSEAHQVLTANGPRSRVLLETDGGLRTGRDIVVAAL"
     gene            complement(3358..4512)
                     /locus_tag="VLS86_03980"
     CDS             complement(3358..4512)
                     /locus_tag="VLS86_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016471916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSO49681.1"
                     /translation="MATSDRLAPFGVSIFSEMTALAQEHQAINLGQGFPNWDGVDFVK
                     EAARLSLQQGGTDQYPPSQGIPALREAVALRYGPLLGRALDPAREVTITCGCTEALAA
                     SFLGLIDPGDEVVLIEPFYDAYPVYVAMSGAIPRYVTLRPPRFELDLDQLRGVISSRT
                     KAIVVNNPHNPTGRVFSDEELSAIARLCVEYDVIAITDEVYEEMTYNVPHLRLANYEG
                     MWERTLTLSSVGKTFSLTGWKVGWGIAPEGLTTGLRSAHQFLTFTTPTPVQHGAVAAM
                     RAPDSFYQDLRSGYLRKRDLLADGLEAVGFDVHRPQGTYFMMAGFEEFGFTDDRAFCR
                     HLVETARVVAIPPSFFYHRREDGSSLVRFAFCKDESILSDALDRLRVLAG"
     gene            complement(4499..>4736)
                     /locus_tag="VLS86_03985"
     CDS             complement(4499..>4736)
                     /locus_tag="VLS86_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HSO49682.1"
                     /translation="LVANPSVRVQIRGSRTSLQARTADAAERLQWWPKVVDAYHGYSV
                     YQSRTDREIPVVILEQASDRSPVSRQDQGEDGHL"
BASE COUNT          844 a         1534 c         1600 g          758 t
ORIGIN      
        1 agcgcggcga ccacgatgtc gcggccggtg cgcaagccac cgtcggtctc gaggagaacc
       61 ctggatctcg gcccgttggc ggtcagcacc tggtgtgcct cggacaaccc gagctcccac
      121 ggtgagccgg cgtgcttgat cgacaccagt ggcgaggcgc cggtgccgcc ctcggatccg
      181 gagatcacaa tgacatcggc gtccgccttg gccacgccga cggcgatggt ccccaccccc
      241 ggctcgctga ccagcttcac cgagacccgc gccgtcggct tgaacgcctt gagatcgaag
      301 atcagctccg ccaggtcctc gatcgagtag atgtcgtgat gcggtggtgg cgagatcagg
      361 gtgacaccag gctccgtgta gcggaggcgg gcgatctcgt cggacacctt gtgaccggga
      421 agctggccgc cctcgcccgg cttcgagccc tgtgccatct tgatctggag ctcgtccgcc
      481 gagtgcagat aggcaggggt caccccaaaa cgtcctgaag ctacctgctt gatcgccgag
      541 ttcttcggag tcccgaaccg ggcaggatcc tcaccaccct caccggagtt ggaatgcccg
      601 ccgatcatgt tcatcgcctc ggcgagagcg acgtgcgcct cggaggacag ggcgccgagc
      661 gacatcgctg cagtggtgaa ccggcgcatg atctgttcgg ccggctccac ctcatccagt
      721 ggcaccggct cccgctgcac gaaagtcagc aggtcgcgga ccagggcggg ctcccgatcc
      781 gagatcatct cgatgtaacg gtcccaatcc tcggggtcgc cggaccgcac cgacttctgc
      841 aaggcgagca ccaccatcgg gctcgtgacg tgggtatcgg agccgcggcg gtgcttgtag
      901 aacccacccg aatccggaga gcccccggaa taggactcat gacgctgcag tgcctgacgc
      961 gccagctcct cgagcccgat cccccgcaac cggcgcggtg cattgcggaa cgccttgcga
     1021 cacaccgcct cggagagacc gatgacctcg aacaactccg agccccgata ggcggagagg
     1081 gtgcagatgc ccatcttcga catgatcttg agcaaaccgc tctccatggc cagccggaag
     1141 ttctcctgcg ccggaaccgg gtcgacgtcg atcgccccgg atgcggcgag atcgcgtgcg
     1201 tgctcgatgg ccagataggg gttgactgca gatgcgccaa agccgatcag acacgccagg
     1261 tcgtgggggt cccgcggctc cccggagacg acgaccagcg agaccgagcc gcggacgccc
     1321 tcgtcgatca gatgagggtg caccgccccc agcgcgagca gaatcgggat cgcagctcga
     1381 ttggcatccg tctcccggtc ggagaggacg atgatccggg ctccgagccg caccgcctgg
     1441 gccgcctcgt cgcagatctc gtcgagccga ccctccatcc cgtctgggcc tgacagcacc
     1501 ggccaggtgg ttgcgatcca atgggagaag aagcggggat cgccggagcg aacgatcgcc
     1561 tcgagctcgg cctcggacag gatcgggctg gcgagctcga tcagatgggc ctgctgcggg
     1621 gtgtcctcga gaagagggcc gcgacgcccg agctggatgc gaagcgacat caccagcttc
     1681 tcccgaatcg ggtccatcgg cgggttggtc acctgggcga actgctcgtg gaagaactgg
     1741 gtgagacgtc gtggccggct ggagaggacg gcgagacctg tgtcgtcacc catcgatccc
     1801 aacgggttct cgccgcgagc catgtcggcg atgatcagcc tgcgctcctc ggcggtgtat
     1861 ccgaagaccc tgctcagggc ggcggcgtcg aagcggtcgt cggccgtgcc gtcgaaggag
     1921 gcctgaatgc gcagtgtctc ggtgctgatc cagtcgccgt aggggcgggc cctggccagc
     1981 gccgccttga cctgatccga gtgctgcacc tcaccactca ccccgtcgaa gaagatcacc
     2041 tctcccggcc ccagctgccc ggtagcggtg gcccgtgcct cttcctcggg acacaccccg
     2101 gcctcggagg caaccagcac cacctcggga gtgatcgccc aacgtgccgg gcgaaggccg
     2161 ttgcgatcca tgccgaccag cagcgaggtc ccatcggtgg ccgcgatcgc cgccgggccg
     2221 tcccagggct cggtgagaaa ggcgtggtat gcgtagaaag acgccagcgc ggggtcgagg
     2281 tcgggcacgt tctcccaggc cgccggcatc agcatctcct tgacgtgggt cagcgacctg
     2341 ccgctccgca tcaggagctc gaagacgttg tccagacttc ccgagtcgga gattcccggt
     2401 tggaggaagg ggaacacgtc gggcagccgg tcgccccaaa cccctggagt ggcgcccttc
     2461 tccctggcga ccatccacga ccggttggac tggatggtgt tgatctcgcc gttgtgggcg
     2521 agtgctcgaa acggctgtgc gttgtcccat gagggaaagg tgttggtcga gtacctctga
     2581 tggaagatgg cgaaggcggt ctcgtaatca gggtctctca ggtcccagta gaaattggag
     2641 atatgagagg cggtgaacag gcccttgtaa acgacggtgc gactcgaggc ggaagggatg
     2701 gatagacccg gtgtcgcggc ccgctcgatt ctcttccggg ccaggaacag gctgcgctcg
     2761 aagccggccg agtcaccact gccggtgact atcgcctgct cgatcaaggg gaggacgagg
     2821 cgggcgtggt cgcccagcac cgaagggtcg atcggcaccg acctccagaa gagcacccgc
     2881 atcccctccg cttcgatgga ctgggtcacc accgaccgag aggcagccgc gtcggtgggt
     2941 gacaggaagc acatgacgac gccgaggtgc tcaggtgaca ccacgagccc gtgagcgctc
     3001 agctccctgg tgatgagacg gagcgggatc tgggtgagca acccggcgcc gtccccggtc
     3061 ccatccgcgg cgcgcgctcc ccggtggtcc agccgctcca ggcactcgac cgcgagacgg
     3121 gtcatccggt agctcggggc ccgatccagg gcggcgatga acccgacgcc gcatgcgtcg
     3181 cgatctggcc cgggatcgaa gaagggtggc gtcattgctc ccatggtctc taggggagcg
     3241 attaagtgta aaccgggaac gcaggggtca ggccgtgatt ggccacctgc caacgctgaa
     3301 gccggttggg atggccagag ccggtgcgtg gaccggaaac tccaatcggg cacgagatca
     3361 gccggcgagc actcggagtc gatcgagggc gtcggagagg atcgactcgt ccttgcagaa
     3421 ggcgaaccgc accagtgacg atccgtcttc ccgacggtga tagaagaagc tgggcgggat
     3481 cgcgaccacc ctggccgtct ccaccagatg gcggcagaag gcgcgatcgt cggtgaagcc
     3541 gaactcctcg aagccggcca tcatgaagta ggtgccctgg ggccggtgga catcgaagcc
     3601 tacggcttcg agcccgtccg ccaggaggtc gcgctttcgg aggtagccgg atcgcaggtc
     3661 ttggtagaag ctgtccggcg ccctcatcgc ggccaccgcc ccgtgttgaa ccggggtcgg
     3721 cgtggtgaag gtcaggaact ggtgagccga cctcaggccc gtggtgagcc cctccggtgc
     3781 gatcccccac cccaccttcc agccggtcag cgagaacgtc ttgccgacag aggacagggt
     3841 gagggtccgc tcccacatcc cctcgtagtt cgccagccgg agatggggga cgttgtaggt
     3901 catctcctcg tagacctcgt cggtgatcgc gatgacgtcg tactcgacgc agagacgggc
     3961 aatcgcactc agttcctcgt cactaaagac ccggcccgtc gggttgtgcg ggttgttgac
     4021 gacgatcgcc ttcgtccgcg aggagatcac cccccggagt tgatcgagat ccaactcgaa
     4081 ccgaggagga cgcagcgtga cgtaccgggg aatcgccccc gacatcgcca catacaccgg
     4141 ataggcgtcg tagaacggct cgatgagcac gacctcgtcg ccggggtcga tgagaccgag
     4201 aaagctggca gccagggcct cggtgcagcc acaggtgatg gtcacctcgc gtgccggatc
     4261 gagtgctcgg cccagcaagg ggccgtagcg aagggccacg gcctctcgga gagcggggat
     4321 cccctggctc ggtgggtact ggtcggtgcc accctgctga agcgagagcc gtgccgcctc
     4381 cttgacgaaa tcgacaccgt cccagttcgg gaagccctgg ccgaggttga tcgcctggtg
     4441 ctcctgggca agagcggtca tctcgctgaa gatgctcact ccaaacgggg ccagacggtc
     4501 agaggtggcc atcttcgcct tgatcctgtc tgctgacggg ggaccgatcc gaagcctgtt
     4561 cgaggatgac caccgggatt tcgcggtcgg ttcggctctg gtagacggag tacccgtggt
     4621 aggcgtcgac gaccttgggc caccattgca gacgctccgc cgcgtcagcc gtccgggcct
     4681 gaagcgacgt ccgtgagcct cgaatctgca ctcgaaccga ggggttggcc accagg
//
LOCUS       DATITW010000279         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_695120, whole genome shotgun sequence.
ACCESSION   DATITW010000279 DATITW010000000
VERSION     DATITW010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695120"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..587
                     /locus_tag="VLS86_03990"
     CDS             99..587
                     /locus_tag="VLS86_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HSO49683.1"
                     /translation="MKAYLDIETSFDGAITIVGCHLVDRGLIQLVGGMVSDVSVWRAL
                     EGVETICTYNGSRFDLPVIRRRLGLDLAKEFRSHDLMYDCWRNGLFGGLKRVEEQLGI
                     ARRSKGIDGWDAMRLWSRYEDGGDREALEILLAYNRDDVLNLPVLEARLSEIEGTYAG
                     RD"
     gene            571..1878
                     /gene="mtaB"
                     /locus_tag="VLS86_03995"
     CDS             571..1878
                     /gene="mtaB"
                     /locus_tag="VLS86_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HSO49684.1"
                     /translation="MRVAIKTLGCRQNQYESEALQEALRRDGNRPVGPGEEADLFIIN
                     TCTVTKEADADSRQMIRRAIRDTPSARVVVTGCYAQVAASEIASIPGVDLVAGNGEKT
                     RLLELIRGLAEKGPPLIAVGDIQRIRTFDPLPPPIAGSRSRAFLKVQDGCNYRCTFCI
                     IPETRGPNRSQSKEAALWDLRALLGAGYPEVVLTGIHLGTYGRDLPTRGSIAGLLSEM
                     LEVAAPARIRLSSLDPHEVGEDLIGLFGRFGNLCRHLHLPLQSGVDRVLKLMRRVYKA
                     EAFRRLVERLAEAVPGIAIGTDVIVGFPGEGEAEFENTYKLLEGLPIAYLHVFSYSPR
                     DLTVAASMPDQIHKPVKAARSFALRSLSETKWHRFRRELLDQSLAAVVLDRHDTRTGH
                     LEALTDNYVTVAFDAGETSVGRTVDLTIENVTERRTFGRMSGV"
     gene            1945..2811
                     /gene="mtnP"
                     /locus_tag="VLS86_04000"
     CDS             1945..2811
                     /gene="mtnP"
                     /locus_tag="VLS86_04000"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HSO49685.1"
                     /translation="MTEGLIGIIGGSGLYDMEGLESIEERRIETPFGPPSDVYVTGLL
                     GGRRIAFLARHGRGHRLMPSELNFRANIFGFKLLGAEWVISAAAVGSMREELAPLHIV
                     IPDQFFDRTKGRISTFFGDGIVVHVSFADPTCPVLGRQLLSAGEAVGARMHWGGTYLC
                     MEGPQFSTRAESRIYRSWGVDVIGMTNLQEAKLCREAEICYATMALVTDYDVWHDTEA
                     DVSVEAVVVVLKQNAETAKAIIKQAVSSFPTKREGCPCPTALKDAIITAPAAIHPEVR
                     QRLRPIIGKYVP"
     gene            2920..>3197
                     /locus_tag="VLS86_04005"
     CDS             2920..>3197
                     /locus_tag="VLS86_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HSO49686.1"
                     /translation="MSILVVGSVALDSVRTPFGEVKEALGGSATYFSTAASFFADVRV
                     VAVVGEDFPEEHLTFLKSRSIDLRGLKQVPGRTFRWSGEYGFDLNDAET"
BASE COUNT          580 a          911 c         1086 g          620 t
ORIGIN      
        1 aaaggaatcg tcgtccgcgc tccggcaggc actgcgggtt gcttgccttt ccccttttga
       61 attgaaattt gcgggacctg tggtaagctc gtgttccgat gaaggcctac cttgacatcg
      121 agacctcatt cgacggagcc atcactatag ttggttgtca tctcgtggat cggggcctga
      181 tccagttggt cggcggaatg gtcagcgacg tctcggtctg gcgagcgctg gaaggggtgg
      241 agacgatctg cacctataac ggcagccgtt tcgacctgcc ggtgatccgc cggcgattgg
      301 gcctcgatct ggccaaagaa ttccgatccc acgacctgat gtatgactgc tggcgcaacg
      361 gcctgttcgg cgggctgaag cgggtagagg aacagcttgg gatcgctcgc cgttcgaaag
      421 ggatcgatgg ctgggacgcc atgcggctct ggtcacgata cgaagatggc ggcgataggg
      481 aggcgttgga gatcctcctg gcctacaacc gggacgatgt cctgaacctt ccggttcttg
      541 aagcccgcct atcggagatt gaggggacct atgcgggtcg cgattaagac gttgggctgc
      601 cgccagaatc agtacgagag cgaagccctc caggaggcgc tgcggcggga tgggaacagg
      661 ccggtcggtc ccggtgagga agccgacctc ttcatcatca atacgtgcac agtgaccaag
      721 gaggccgacg ccgattcgag acagatgatc cggcgggcga tcagggacac cccttcagcc
      781 cgtgtcgtcg tcacaggctg ctacgcccag gtggccgcca gcgagatcgc atcgattccg
      841 ggcgtggatc tggtggcggg caacggggag aagacccgac ttctcgagtt gatccgcggt
      901 ctggcggaga aggggccgcc gctgatcgcc gttggtgata tccagcgaat ccgcaccttt
      961 gacccgcttc cgccgccgat cgcggggtct cgaagccggg cgttcctgaa ggtccaggat
     1021 gggtgcaatt accgctgcac gttttgcatc atcccggaga cccgcgggcc caaccggagc
     1081 cagtcgaagg aggccgcgct gtgggacctc cgggccctgt tgggcgccgg gtaccctgag
     1141 gttgtcctga ccgggatcca cctcggaacg tatggacgcg atcttccgac ccgaggttcg
     1201 attgctggct tgctcagtga gatgctggag gtggctgcgc ccgcgaggat acgcctgagc
     1261 tccctcgacc cacacgaggt gggagaggac ctgatcggcc tcttcgggcg tttcgggaac
     1321 ctgtgtcgtc acttgcacct gccgctgcag agtggggtcg atcgggtgct gaaactcatg
     1381 cgacgtgtct acaaggctga ggcatttcgt cggctggtgg agcggctcgc ggaggcggta
     1441 ccggggatcg ccattgggac cgacgtcatc gtcggatttc caggtgaggg cgaggcggag
     1501 ttcgagaaca cctacaagct cctggaaggc ctgccgatcg cctatctcca tgtcttcagc
     1561 tactccccgc gggacctgac tgtcgccgcg tcaatgccgg atcagatcca caagcctgtg
     1621 aaagcggcgc ggagcttcgc cctccggtcg ttgagtgaga cgaagtggca tcggtttcga
     1681 cgcgaactgc tggaccagtc cctcgcggct gtcgtccttg acaggcatga tacgcggacc
     1741 ggtcacctgg aggcgctgac agataactat gtcactgttg ccttcgatgc cggtgagacc
     1801 tcggtgggcc ggacggtgga tctcacgatc gagaatgtta ccgaacggcg gacttttggt
     1861 cgcatgagcg gggtatgacc atatcgacct gccgttgagc ggaccgatcg ggaggctgca
     1921 ggtcgggtct caagtggaga caaaatgacc gaagggttga tcggcattat cgggggaagc
     1981 gggctctacg acatggaggg cctcgagtcg atcgaagagc gccgcatcga gacacccttt
     2041 ggaccgccgt ccgacgtcta cgtcaccggg ttgctcgggg ggcggcggat cgcgttcctg
     2101 gcgcgacatg gccgcggcca tcgcctgatg ccctcggagt tgaacttccg agccaatatc
     2161 ttcggcttca aactgctcgg ggcggagtgg gtgatctccg cggccgcggt cggaagcatg
     2221 cgcgaggaac ttgcgcctct gcatatcgtg atcccggatc agttcttcga tcggaccaaa
     2281 gggagaatta gcaccttctt cggcgacggc atcgtggtcc acgtgagctt cgccgacccg
     2341 acatgtccgg tcctcggaag gcagttgctc tcggccggcg aggcggtcgg cgcgcgcatg
     2401 cattggggcg ggacgtacct ctgcatggag gggccgcaat tctcgaccag ggctgagtcc
     2461 cggatctatc gaagctgggg agtggatgtc atcgggatga ccaacctgca ggaggcgaag
     2521 ctctgccgcg aggctgagat ctgctacgcg acgatggcgc tcgtgaccga ttatgacgtt
     2581 tggcacgata cggaggcaga cgtgtcggtc gaagcagtgg tggtcgtcct gaaacagaac
     2641 gccgaaacgg ccaaggcgat catcaaacaa gccgtgtcat cgttcccgac gaagcgagag
     2701 ggctgcccct gcccgaccgc actgaaggac gccatcatta cggctcctgc ggcgattcat
     2761 ccggaggttc gccagcggct gcggccgatt atcgggaagt atgttccgta aggttccagg
     2821 tgccacgttc caggttccat gttggatgga tcctttattg aacccggaac cccgaacccg
     2881 gaacttggaa ccccgaaccc ggaacaccgc ggagcggaca tgagtatcct ggtcgtcggg
     2941 tcggtcgcgc tcgactccgt tcgcactccg ttcggcgagg tgaaggaggc gctcgggggg
     3001 tcggcgacgt acttttcgac ggcggcgagc ttcttcgccg acgtccgggt tgtcgcagtg
     3061 gtgggggagg acttcccaga ggagcatctg acattcctga agagccggtc gatcgatctt
     3121 cgggggctga agcaagtgcc gggccggacc ttccggtggt cgggggagta cggcttcgat
     3181 ttgaacgacg ccgagac
//
LOCUS       DATITW010000280         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_775124, whole genome shotgun sequence.
ACCESSION   DATITW010000280 DATITW010000000
VERSION     DATITW010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775124"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /gene="fecE"
                     /locus_tag="VLS86_04010"
     CDS             <1..201
                     /gene="fecE"
                     /locus_tag="VLS86_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018532598.1"
                     /note="Part of the FecBCDE citrate-dependent iron (III)
                     transport system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HSO49687.1"
                     /translation="AHVANHVLLLSGRVVASGAPEAVLTIDNLIAAYGPKLLHVEGEQ
                     LFIDDPAHRPVPGRHTHRDRSS"
     gene            complement(205..468)
                     /locus_tag="VLS86_04015"
     CDS             complement(205..468)
                     /locus_tag="VLS86_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSO49688.1"
                     /translation="MNIYVGNLPFNTTAADLEGLFGEYGTVESAAIINDRETGRSRGF
                     GFVEMPNDAGAKAIQELDGADFNGRNLKVNEARPREERPRHGR"
     gene            complement(643..1152)
                     /locus_tag="VLS86_04020"
     CDS             complement(643..1152)
                     /locus_tag="VLS86_04020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSO49689.1"
                     /translation="MTKQYPAPPELSIDLRTTYTATLDTSHGEIVIELDAVRSPQTVN
                     NFVFLARDGYYDGVIFHRVIENFMIQGGDPTGTGMGGPGYKFRDEIEGRGTYNRGTVA
                     MANAGPHTNGSQFFICHADTGLPHSYTIFGRVTSGMDTVDSIATTATDSSDRPETEVV
                     INAVTIAES"
     gene            complement(1191..1775)
                     /locus_tag="VLS86_04025"
     CDS             complement(1191..1775)
                     /locus_tag="VLS86_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015224803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSO49690.1"
                     /translation="MAVKSTMLALGTKAPDFTLPDVVSGREVSLSDFEGKDGLLVMFI
                     CNHCPYVKHVRAGLARFGADYQDSALGIVAISANDPGQHPDDSPEAMKAEAITHGYTF
                     PYLFDEGQDVAAAYTAMCTPDFFLFDSNRELVYRGRFDESRPDSGIPVTGADLRAAAD
                     AVLSGDPVNDEQWPSMGCSIKWKSGNTPVYFGAA"
     gene            complement(1785..>2165)
                     /locus_tag="VLS86_04030"
     CDS             complement(1785..>2165)
                     /locus_tag="VLS86_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49691.1"
                     /translation="LVARKARACNLFGRPEVVAHKVRVLAEHCAEVGRDPEEIEVTHL
                     VDAMTARDRNGLRQRVEQLRGRSMSAAEYAARHNAGTVDDQIAHLSSYRDAGASHTIV
                     VLPDLHLEDSIESFAEVIQRMSGP"
BASE COUNT          355 a          672 c          729 g          409 t
ORIGIN      
        1 gcccatgtgg ccaatcacgt gttgcttctc tcgggtcggg tggtggcctc aggcgcgccg
       61 gaagcggtcc tgacgatcga caacctgatt gccgcctacg gcccgaagtt gctccacgtc
      121 gagggggagc agctgttcat cgacgatccg gcgcatcgcc cggtccccgg gcgccacacc
      181 caccgggatc gttccagcta gccctcagcg tccgtggcgg gggcgctcct cgcggggacg
      241 tgcctcgttg accttgaggt tgcggccgtt gaaatcggcc ccgtccagtt cctggatcgc
      301 cttggcgccg gcgtcgtttg gcatctcgac gaatccaaaa ccacgcgacc gaccggtctc
      361 ccggtcattg atgatggccg ccgactcgac cgtgccgtac tcgccgaaca ggccctcgag
      421 gtcggcggcg gtggtgttga agggaagatt ccccacgtag atgttcatga ctcccgtcat
      481 cctttctaat gaagggcaac ttggcagccc ttcgccatgt tgtccggtac gaccctgcgc
      541 tcaatccggt cgcaatgcag gacgggaagc caacgagtcg ccgccgaagc agcggcggga
      601 acgctagtgg aaaagaatcg aatgcgggag aaatcctggg ggtcagctct cggcgatggt
      661 caccgcgttg atcaccactt cggtctccgg gcgatcgctg gagtcggtgg cggtggtcgc
      721 gatcgagtcg accgtgtcca ttccggaagt gaccctcccg aagatggtgt aggagtgggg
      781 aagcccggtg tccgcatgac agatgaagaa ctgggacccg ttggtgtgtg gcccggcatt
      841 ggccatggcc acggtgcccc ggttgtaggt gccgcgacct tcgatctcgt cccggaactt
      901 gtacccgggg ccgcccatgc cggtgccggt cgggtccccg ccctggatca tgaagttctc
      961 gatcacccgg tggaagatca ccccgtcgta gtagccatcc ctggccagga agacgaagtt
     1021 gttgacggtc tgcggagagc gcaccgcgtc cagctcgatc acgatctcac cgtggctggt
     1081 gtcgagggtc gcggtgtacg tggtgcggag gtcgatgctc agttccggag gtgccgggta
     1141 ttgcttggtc aaggttgttc ctctcgtggt tctggcgatt ggctctgggc tcaggccgcg
     1201 ccgaagtaga ccggggtgtt gcccgacttc catttgatcg agcagcccat cgatggccac
     1261 tgctcgtcgt tgaccgggtc accggacagg acggcgtcgg ctgcggcccg aaggtcggcg
     1321 ccggtcaccg ggatcccgct gtcaggccgg gactcgtcga agcggccccg gtacaccaat
     1381 tcccggttcg aatcgaagag gaagaaatcc ggcgtgcaca tcgcggtata ggcggcggcc
     1441 acgtcctgac cctcgtcgaa caggtaggga aaggtgtagc catgggtgat ggcctcggcc
     1501 ttcatcgcct ccgggctgtc atcgggatgc tgacccgggt cgttggcaga gatggcgacg
     1561 atgcccaggg cgctgtcctg gtaatcggcg ccaaaccggg ccagaccggc gcggacgtgc
     1621 ttcacgtacg ggcagtggtt gcagatgaac atcaccagca gcccgtcctt cccctcgaag
     1681 tcggagagtg agacttcacg ccccgacacc acgtcgggaa gggtgaagtc gggggccttg
     1741 gtgccgaggg cgagcatcgt tgatttgaca gccatgagcc aagtctaagg gccggacatt
     1801 ctttgaatga cctcggcgaa ggactcgatg ctgtcttcga ggtggagatc gggcaggacg
     1861 acgatcgtgt gactcgcacc ggcatcccgg taggacgaga ggtgggcgat ctgatcgtcc
     1921 acggtcccgg cgttgtgccg cgccgcgtac tcggcggccg acatgctgcg tccccgaagc
     1981 tgttcgaccc gctgccgcag cccatttcgg tctctcgccg tcatcgcgtc gacgaggtgg
     2041 gtgacttcga tctcctccgg gtcgcggccc acctcggcgc aatgctcggc gagcacccgc
     2101 accttgtgcg caaccacctc aggtcgcccg aacaggttgc aggctcgggc cttgcgggcg
     2161 accag
//
LOCUS       DATITW010000281         1585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_735135, whole genome shotgun sequence.
ACCESSION   DATITW010000281 DATITW010000000
VERSION     DATITW010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735135"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /locus_tag="VLS86_04035"
     CDS             complement(<1..1489)
                     /locus_tag="VLS86_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HSO49692.1"
                     /translation="MTKPLESIRRLPAVGVLAERQMGGFLAISALVGVGVGLGAAALI
                     LLVDWLEEFFLVFAIDQGHAWIFLTVPTGFLFAWFLAKRFAPEIAGDGVPEAIASLEV
                     HGGRMRGRTIPLKILATAATVGTGGSGGREGPIVQIGAAVGSVMSRRFRLGEDQVRSL
                     VAAGAGAGIGATFNAPIAGMLFALEVILSSFAARHMSAIVLASITAAVTTRTIIGPEL
                     AIRAGTYELGNFAELFLYAGLAIVVVAVGVAFLKLLDRLETFTRAQARSLGFLRPLLA
                     GLIVATLIFFEPRLFGTGQSTINDLLLGERVSSITGAVTDFWWVFLLLALGKVVATSF
                     TITSGASGGAFMPSLFMGAAVGTGFARLIGPVWNLTPAIEPGAFAVVGMAAMFAVVGR
                     APLTAILIVFEVTGARDYQLILPLMLTATLGTFLAERFNRDSVYSMALRHRGIKVRQT
                     GQVDLLDTVTVGSVMATAPLICSPDDPLGEVEQRLHRARSHGMPVV"
BASE COUNT          330 a          552 c          496 g          207 t
ORIGIN      
        1 cgaccaccgg catgccatga gaacgagccc ggtgcagtcg ctgctcgacc tcgcccaacg
       61 ggtcgtccgg gctgcagatg aggggggcgg tggccatgac cgatccgacg gtgacggtgt
      121 cgagcaggtc cacctggccg gtctgacgga ccttgatccc gcgatgccgc agtgccatcg
      181 aatagaccga atcgcgattg aaccgctcgg caagaaacgt gcccagggtg gcggtcagca
      241 ttaagggaag gatcagctgg tagtcacgag cacccgtgac ctcgaagacg atcaggatcg
      301 cggtcaatgg ggctcggccc accacggcga acatcgcggc catccccacc acggcaaacg
      361 cccccggctc gatcgccggg gtcagattcc acaccggccc gatcaaccgg gcgaaaccgg
      421 tgccgactgc cgcccccatg aacagcgagg gcatgaaggc gccacccgac gcccccgacg
      481 tgatggtgaa actggtggcg acgaccttgc ccaatgcgag gaggaggaac acccaccaga
      541 aatcggtgac cgctccggtg atcgacgaca ccctctcccc gagcagcagg tcgttgatgg
      601 tgctctgccc ggtgccgaac agtcttggct cgaagaagat cagggtggcg actatgagac
      661 ccgccagcag ggggcgcagg aaaccgagag agcgggcttg tgccctggtg aacgtctcta
      721 gacgatcgag cagcttgaga aaggcgacgc cgacggccac cacgactatg gccagaccgg
      781 catacagaaa cagctcggcg aagttgccca gctcgtaggt gccggcccgg atcgccagct
      841 cggggccgat gatggtcctg gtggtgaccg ccgccgtgat cgacgccagc acgatcgccg
      901 acatgtgccg cgccgcgaac gaagacagaa tcacctccag cgcgaacaac atgccggcga
      961 tcggcgcatt gaacgtggcg ccgatgcccg ccccggctcc ggccgccacc agtgacctca
     1021 cctggtcctc gccgaggcga aacctccggg acatgaccga cccgacagca gccccaatct
     1081 ggacgatcgg cccctcgcgg ccccccgacc cgccggtgcc caccgtggcc gcggtcgcca
     1141 ggatcttgag cgggatggtc cgtccccgca tccgcccccc gtggacctcg agcgaggcaa
     1201 ttgcctctgg caccccgtca cccgctatct cgggtgcgaa gcgcttggcc aggaaccacg
     1261 cgaagaggaa gcccgtgggc acggtgagga agatccaagc gtgcccttga tcgatggcga
     1321 acaccaggaa gaactcctcg agccagtcga ccaggaggat cagggcggcg gcacccaacc
     1381 cgacaccaac gccgaccagc gccgagatcg caaggaaacc ccccatctgt cgctcggcga
     1441 gtacacccac ggcgggaagg cggcgaatgc tctccagcgg cttggtcacc ggcggcaggg
     1501 tatcccaatc cctgaaactc cccggtgtcg tccagaatga caccgtgaaa ttcaggtctc
     1561 acatcacccg aaccctcgac cccac
//
LOCUS       DATITW010000282         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_775161, whole genome shotgun sequence.
ACCESSION   DATITW010000282 DATITW010000000
VERSION     DATITW010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775161"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(129..238)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(284..1450)
                     /locus_tag="VLS86_04040"
     CDS             complement(284..1450)
                     /locus_tag="VLS86_04040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HSO49693.1"
                     /translation="MSKRRVVVTGIGVVGAPGVGMDDLWKGLQVAPAAPGPHVVNNWD
                     PEPWLPKREHRRLDRFTQFALVATHEALEQSGQLDIDPNRVTVAVATGIGGLGSLEEL
                     VHGSEADDPRVSPFLLPMMMANAAAAGISIKYGFGGQVSTPVVACAAGTQAILDGLRQ
                     IQWGYADAAVVGGAEAAARRSAREGFLAARALSPSGHARPFDVDRDGFVMGEGAGILI
                     IEAAEIAEARGAKALAVLSGGASTADAHHITAPHPEGDGAERAIRLALEDAGLSPRDV
                     GYVNAHGTGTDLNDRTEGIVIDRIFEDQPAVSSIKGTTGHALGASGSIEAAVVVEAMR
                     RRELPPNIGLAHQDPEIPLTDIVREPRDWDPLPSITNSFGFGGHNTVLAFTPVD"
BASE COUNT          250 a          552 c          538 g          284 t
ORIGIN      
        1 actcatgcgc gatgagggtg aggctatcgg gttggccgcc ctcggatcct ccacaggcgg
       61 cgaccaagag cagggtggtg gtcgcagcga cgaacttgcg cattctcatc agctcctcgg
      121 tttgtcggga gcgatgaggc gcgggtccct acgccggcat tacccgggtc aggttcatcg
      181 gtcggggcct cgcaggcccc ctctcagccc ggtgtcaccg agctcccgta gacaaccagg
      241 gtagccgagt tcaacgtctg ggccgtacct gcccccccgg gcgtcagtcg acgggggtga
      301 aagcgagcac ggtgttgtgc ccgccgaagc cgaaagagtt ggtgatggac ggcaacggat
      361 cccagtccct cggctcgcgc acgatgtcgg tgagagggat ctccgggtcc tggtgggcga
      421 gtccgatgtt gggcggcaac tcgcggcgtc gcatcgcctc gaccacgact gccgcctcga
      481 tcgatcccga ggcgccgagg gcgtgcccgg tggtcccctt gatcgaggag accgccggct
      541 gatcctcgaa tatccggtcg atgacgatgc cttcggtgcg gtcattcagg tcggtcccgg
      601 tcccgtgagc gttgacgtac cccacgtcgc gtgggctcag accggcgtct tccagcgcca
      661 gtctgatagc tcgctccgcg ccgtcgccct caggatgggg agctgtgatg tggtgggcgt
      721 ccgcggtcga ggcgccgcca ctcagcacgg cgagcgcctt ggccccacgg gcctctgcga
      781 tctcggccgc ctcgatgatc aggatccccg cgccctcccc catgacgaag ccgtcacggt
      841 cgacgtcgaa gggacgggca tgcccgcttg gcgacagggc gcgggccgcc agaaagcctt
      901 cccgagcgga acgtctggcg gcggcctctg ccccgccgac caccgctgcg tcggcgtaac
      961 cccattggat ctgtctcagc ccgtcgagga tggcttgggt gcccgcggcg caggcaacca
     1021 ccggtgtcga cacctgaccg ccgaacccgt acttgatcga gatcccggcg gcggctgcgt
     1081 tcgccatcat catcgggagc agaaacgggg acacccgggg atcatcggcc tcggacccgt
     1141 ggaccaactc ctcgagagag cccagaccgc cgatcccggt ggccacagca accgtcaccc
     1201 ggttcgggtc gatgtcgagt tgccccgact gctccagcgc ctcgtgtgtg gcgacgaggg
     1261 cgaactgggt gaagcggtcg agccgcctgt gctcgcgctt gggcagccac ggctcgggat
     1321 cccagttgtt caccacgtgg gggcctggcg ccgccggcgc cacctgcagg cccttccaca
     1381 ggtcgtccat cccgacaccg ggcgccccga cgacgccgat gccggtcacg acgactcttc
     1441 gtttgctcat ggctctaatt gtgcccgttt tctgaggttg gaaaaacaga tcgttgcagg
     1501 gtcctcattc cattgatcca gcggtcgtag tcatttgcct tgttctggaa gaagcgctcc
     1561 acctcgggat gggggaggat caagaacttc tcctcggcca gacccgcgac cacggcttct
     1621 gcca
//
LOCUS       DATITW010000283         1537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_454704, whole genome shotgun sequence.
ACCESSION   DATITW010000283 DATITW010000000
VERSION     DATITW010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454704"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VLS86_04045"
     CDS             complement(<1..520)
                     /locus_tag="VLS86_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HSO49694.1"
                     /translation="MSQEHASRRRRRLTATVTDLEVAIQRAFLVGVALPGMNGIDSER
                     SLEELALLTDTAGSEPVDQELIRRTRIDPATYVGSGKAEELATLTKALDIDVVIFDNQ
                     LTPAQQRNLQKLFGCDVVDREGLILDIFAQHAHTRVGSLQVELALHRYNLPRLRGKGK
                     SMSQQGAGILARG"
     gene            complement(588..890)
                     /locus_tag="VLS86_04050"
     CDS             complement(588..890)
                     /locus_tag="VLS86_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49695.1"
                     /translation="MTDLPILHDNGYKVMTGWRVGHAPEESTTRSGLLVPIGTKDGND
                     ALTLVDVETGQRFWAVPTGAWRFIWPATGKPVIAVRESQIIAEEQSEHVGDEGQYL"
     gene            1007..>1537
                     /locus_tag="VLS86_04055"
     CDS             1007..>1537
                     /locus_tag="VLS86_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007274584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HSO49696.1"
                     /translation="MADKRTFSEALDYHEQPTPGKVGIRSTKPTATQADLSLAYTPGV
                     AEPCLVIADDANAAFRFTNRGNLVAVVSNGSAVLGLGNIGALASKPVMEGKAVLFKRF
                     ADIDVFDLEIDAVEASDIVRFCEMLAPTVGGINLEDIKSPECFEIEQTLRERLDIPVF
                     HDDQHGTAIIGGAAFLN"
BASE COUNT          254 a          485 c          500 g          298 t
ORIGIN      
        1 gcccgcgggc caggattccg gcgccctgct gtgacatgct ctttcccttt ccccgaagac
       61 gggggaggtt gtagcgatgc agagccagct cgacctgcaa cgagccgacc cgggtgtggg
      121 catgttgggc gaagatgtcg aggatgaggc cctcccggtc gacgacatcg cacccgaaga
      181 gcttttgcag gttgcgttgc tgggcggggg tcagctggtt gtcgaagatg acgacgtcaa
      241 tgtcgagcgc cttggtgagc gtggccagct cctccgcctt gcccgacccc acataggtgg
      301 ccgggtcgat ccgggtgcgt cggatcagct cctggtcgac cggctccgat cctgcggtgt
      361 cggtcaacag ggcgagctct tcgaggctcc tctcggagtc gatgccgttc atcccgggca
      421 gggcgactcc gaccagaaac gccctctgga ttgcgacttc gagatcggtg acggttgccg
      481 tgagacggcg acggcggcgg gatgcgtgtt cctggctcat gtccgtggcg ggttgcttgt
      541 ccgaggatgg tgcccgaatc gcacccggga tgcggggtgt ttatcgctca gaggtactgg
      601 ccctcatcgc ccacatgctc actctgctcc tcggcgatga tctgggactc gcgcaccgcg
      661 atcaccggtt tcccggtggc gggccagatg aatcgccagg cgccggtggg gacagcccag
      721 aaccgctgcc cggtctcgac gtcgaccagc gtcagcgcgt cattgccgtc cttggtgccg
      781 atgggcacca gaagaccgga acgggtggtc gattcctcag gtgcgtggcc gacccgccag
      841 ccggtcatga ccttgtaacc gttgtcgtgg agaatcggca ggtctgtcat cgtcattcgt
      901 cgctcaattg tcgcatgtgt cccccccaac gtcgaagtcg ttgctttctg caggagaaat
      961 cggcggccct gatcggcact cgggatcatt gatataggct ggcccgatgg ccgacaaacg
     1021 cacgttctcc gaggccctcg attatcacga gcagccgaca ccgggaaagg tcgggattcg
     1081 ctcgaccaag ccgacggcga cccaggccga tctcagtctc gcctacaccc ccggtgtcgc
     1141 cgagccctgc ctcgtcatcg ccgacgacgc caacgccgcc ttccgcttca ccaatcgggg
     1201 gaacctcgta gccgttgtgt ccaacggatc cgcggtcctc gggctgggga acatcggggc
     1261 ccttgccagc aagccggtga tggagggcaa ggcggtcttg ttcaagaggt tcgccgacat
     1321 cgacgtcttc gatctcgaga tcgacgccgt cgaggcctcg gacatcgtca ggttctgcga
     1381 gatgctcgct cccactgtgg gtgggataaa tctcgaggac atcaagtccc cggagtgctt
     1441 cgagatcgag cagacgctgc gggagcgcct cgacatccct gtgtttcacg acgaccagca
     1501 cgggaccgcg atcatcgggg gagccgcatt tctcaat
//
LOCUS       DATITW010000284         1826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_294587, whole genome shotgun sequence.
ACCESSION   DATITW010000284 DATITW010000000
VERSION     DATITW010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294587"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VLS86_04060"
     CDS             <1..366
                     /locus_tag="VLS86_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HSO49697.1"
                     /translation="HIDEATKTADVVVSEHQLSLAIGKEGQNARLAARLSGYKVDIRS
                     DTEGVEEEAASADGEATAVVEEPVAIDAEAAPEAAPEAEPVEETPAGEEPPPVTSDAE
                     ADGDDEDAVAEVSDDEESE"
     gene            378..>1826
                     /gene="infB"
                     /locus_tag="VLS86_04065"
     CDS             378..>1826
                     /gene="infB"
                     /locus_tag="VLS86_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HSO49698.1"
                     /translation="MRVYELARDLGVDSKELLKQAQDLGIDVKTASSGLDAEAAELLK
                     LSVAGDQAAETEPGPEPESAPEEAAPVEAAPPAAETEPEVEEQVEQQVEIVSIPPGAT
                     VTQFAEAIGVTPTDVVTELLMRGNPVGVGQTMPAELVEPVAESFGFIVDVGVAPPPPV
                     VAERPVFEDDPSALASRPPVVTVMGHVDHGKTTLLDTIRKTAVVDSEQGGITQHIGAY
                     QVEVNGHPITFIDTPGHEAFTALRARGANITDIVVLVVAANDGVMPQTVEAISHAKAA
                     DVKLIVAVNKVDLPGADPLRVRTELTEHDVITEELGGDVPSVEVSALTGDGVDDLLDV
                     IDLIAQLEEFKANPDTEASGVVVEAQVELGRGPVASVIVQRGTLNTGDSFVAGAVSGR
                     ARALMDDTGQQIKSAGPSAPVQIMGWDDVPAAGDFFRVVDSDREARGMAEERQEELKA
                     ASQAMPSARDRLQGLLDQLRSEEATLNVILKAD"
BASE COUNT          336 a          577 c          650 g          263 t
ORIGIN      
        1 cacatcgacg aggcgaccaa gaccgccgac gtggtggtct ccgagcatca gctgtccctc
       61 gccatcggca aggagggcca gaacgccaga ctcgcggccc ggctctccgg ctacaaggtc
      121 gacatccgat ccgacaccga aggggtggag gaggaggcgg cgtcggccga cggggaggcg
      181 accgcggtgg tcgaggagcc ggtggcgatc gacgccgagg ccgcacccga agccgcaccc
      241 gaagccgagc cggtggaaga gacgcccgct ggcgaggagc cgcctcccgt gacgagcgat
      301 gcggaggccg acggcgacga cgaggacgcc gttgccgaag tgtccgatga cgaagagagt
      361 gagtagagag tcttagcgtg agggtttatg aacttgcccg cgacctgggc gtggactcga
      421 aagagctgct gaaacaggcc caggatctcg ggatagatgt gaaaacggca tcgtcggggt
      481 tggatgccga agctgccgag ctgttgaagc tgtcggtcgc cggcgaccag gcggctgaga
      541 ccgagcccgg gcccgagccg gaatcggcgc cggaagaagc agcgccggtc gaggccgccc
      601 cacccgccgc cgagaccgag cccgaggtcg aggaacaggt cgagcaacag gtcgagatcg
      661 tctcgattcc cccgggagcg acggtgaccc agttcgccga ggctataggt gtcaccccaa
      721 ccgacgtggt aaccgagctg ctgatgcggg gtaacccggt gggggtcggt cagaccatgc
      781 cggccgagct ggtcgaaccg gtggccgagt cgttcgggtt catcgtcgac gtcggggtcg
      841 cccctcctcc gccggttgtc gccgagcgcc cggtcttcga ggacgaccct tcggctctcg
      901 ccagccggcc tccggtagtc accgtgatgg gacacgtcga tcacggcaag accactctcc
      961 tcgacactat tcgcaagacc gcggtggtgg acagcgagca gggcgggatc acccagcaca
     1021 tcggcgccta ccaggtcgaa gtcaacggtc atccgatcac cttcatcgac acaccgggtc
     1081 acgaggcgtt caccgccctg cgcgcccgcg gggcgaacat caccgacatc gtggtcctgg
     1141 tcgtcgccgc caacgatggg gtgatgccgc agacggtgga ggccatatct cacgccaagg
     1201 cggcagacgt caagctgatc gtcgcggtca acaaggtcga tttgcccgga gccgacccgc
     1261 tccgggtgag gaccgagctc accgagcacg acgtgatcac cgaggagctc ggtggtgacg
     1321 tccccagtgt cgaagtgtcg gctctcaccg gcgatggcgt cgacgatctg ctcgacgtga
     1381 tcgatctcat cgcccagctc gaggagttca aggccaatcc cgacaccgag gcctctggcg
     1441 tcgtggtcga ggcacaggtc gagctgggaa ggggtccggt tgcctcggtc atcgtccagc
     1501 gggggacctt gaacacgggt gactcattcg tggccggtgc ggtgtcgggg cgggccaggg
     1561 cattgatgga tgacaccggg cagcagatca agtcggccgg gccctcggcg ccggtccaga
     1621 tcatgggctg ggacgacgtg cccgccgccg gtgacttctt cagggtggtc gacagcgatc
     1681 gggaggcgcg agggatggcc gaggagcgtc aggaggagct gaaggcagcc agccaggcga
     1741 tgccttcagc ccgcgaccgg ctccagggtc tgctcgacca gcttcgttcc gaggaggcca
     1801 ccctcaacgt gatcctcaag gccgac
//
LOCUS       DATITW010000285         1507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_535155, whole genome shotgun sequence.
ACCESSION   DATITW010000285 DATITW010000000
VERSION     DATITW010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535155"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..806
                     /gene="ftsE"
                     /locus_tag="VLS86_04070"
     CDS             114..806
                     /gene="ftsE"
                     /locus_tag="VLS86_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HSO49699.1"
                     /translation="MIRLENVTKVYEGGTIAAHDVSIEMQKGEFTFLVGPSGSGKSTL
                     IRLMLRDEPVTRGKIWVAGKDVTTLPKWKIPYLRRSVGTVFQDYKLLPNKTVGENVGF
                     ALEVLGRPRSVIAPQVDQVLDLVGLADKGHRYPRQLSGGEQQRVSVARAFVNRPPIML
                     CDEPTGNLDPKTSVGIMKLFDRINRTGTTIVMATHDFAIVDAMQRRVVALETGLVVRD
                     EASGRYEEQGPE"
     gene            803..>1507
                     /locus_tag="VLS86_04075"
     CDS             803..>1507
                     /locus_tag="VLS86_04075"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HSO49700.1"
                     /translation="MSRFFFLLREAFVNLKRNFLVVAGAVLAVFISLTLAFGALVVNE
                     VLRINTLAWQEGVHVIAFLKDEGSNGVPAGAHQALLDEVLTWEEVKSAFPVGKAEAWV
                     EYQEIFAGQDELLEIDPTILPASIRIELETIELHQSVKFKLEQQQQVVLRVNTAAQQI
                     EQLQNLSSVLNVLGIGMAVVLGLSAVVLIANTIRLAIYARRDEVEIMKLVGASNWYIR
                     IPFLLEGLIEGVVGAAF"
BASE COUNT          279 a          464 c          484 g          280 t
ORIGIN      
        1 tggcgtcggg ccaatcatga gtcaggaaca gattctgcct agtcaggctc gtcgcgttac
       61 cgtcctcgat tcggcggctg gggccggaag gctgccagac ttcccctgat ccaatgatcc
      121 gactcgaaaa cgtcaccaag gtctacgagg gcggcaccat cgccgcccac gatgtctcca
      181 tcgagatgca aaaaggtgag ttcacctttc tcgtggggcc ttccggctcg ggcaagtcga
      241 ccctgatccg cctcatgctc cgggacgagc cggtcacccg gggcaagatc tgggtggcag
      301 gcaaggatgt caccaccctt ccaaagtgga agatccccta cctccgccgc tccgttggga
      361 ccgttttcca ggactacaag ctcctgccca acaagacggt cggtgagaac gtggggttcg
      421 ccctcgaggt gctgggtcgt ccccgctcgg tgatcgcccc ccaggtcgac caggtcctcg
      481 atctggtggg actggccgac aaggggcaca ggtacccccg gcagctctcc ggtggagagc
      541 aacagcgggt ctcggtggct cgggccttcg tgaatcggcc gccgatcatg ctctgcgacg
      601 agcccaccgg gaacctcgac cccaagacat cggtggggat catgaagctg ttcgaccgga
      661 tcaaccgcac cgggaccacg atcgtcatgg caacccatga tttcgccatc gtcgacgcca
      721 tgcagcggcg ggtggtcgca ttggagaccg gtctggtggt cagagacgag gcttcgggac
      781 gctatgagga gcaaggcccg gagtgagccg gttcttcttc ctgctccgag aggcattcgt
      841 caacctgaag aggaacttcc tggtggtggc gggtgcggtg ctcgccgtct tcatctcact
      901 cacgttggcg ttcggggccc tggtggtcaa cgaggtgctc cggatcaaca ccctcgcctg
      961 gcaggagggg gtgcacgtga tcgccttcct caaggacgag ggctcgaacg gggtgccggc
     1021 cggggcccat caggccctgt tggacgaggt cctcacctgg gaggaggtga agagcgcgtt
     1081 cccggtgggc aaggcggagg cgtgggtgga gtaccaggag atcttcgccg gccaggacga
     1141 actgctcgag atcgacccca ccattctccc cgcgtcgatc cggatcgagt tggagaccat
     1201 cgagctgcat cagtcggtga aattcaaact ggaacagcag caacaagtag tgcttcgggt
     1261 caacaccgcc gcccagcaga tcgagcagct gcagaaccta tccagtgtcc tcaatgtgct
     1321 cggaatcggg atggccgtgg tgctcgggct ctcagccgtg gtcttgatcg ccaacaccat
     1381 ccggctcgcc atctacgccc gtcgcgacga ggtggagatc atgaagctgg tcggagcctc
     1441 caattggtac atcaggatcc ccttcctcct cgaagggctg atcgaagggg tggtcggtgc
     1501 cgcattt
//
LOCUS       DATITW010000286         1802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_735279, whole genome shotgun sequence.
ACCESSION   DATITW010000286 DATITW010000000
VERSION     DATITW010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735279"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VLS86_04080"
     CDS             <1..878
                     /locus_tag="VLS86_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO49701.1"
                     /translation="TTALRVIAGFENAGGSIEIHGRKVLDDGVFVPPEKRRVGMVFQD
                     YALFPHMTVASNVAYGLPRGEREPRVGEVLELVGLTGLEDRMPNELSGGQQQRVALAR
                     ALAPEPEVILLDEPFSNLDASLRDRVRRELRVILTEARTTAVFVTHDQEEALATSDIV
                     AVMKDGRVLQADTPADLYSNPADPWIAEFLGDADVLHSEARNGRADTPVGSFQTDLTG
                     KVSVIIRPENVQISLGETPNAVVAGSEFFGHDQLVTVALHGGTRVRARIGPRPIYRVG
                     DACRVRAIDARIFAV"
     gene            951..1658
                     /locus_tag="VLS86_04085"
     CDS             951..1658
                     /locus_tag="VLS86_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49702.1"
                     /translation="MDSLLALLAALAAAAFAADLWLDYRKRPRPHIAAYATGMSMFAV
                     ATLAFFIGLAAGWTGVVYRTFYLFGAVLNIPFLALGSMFLVVGKRSGHAMAIALGALA
                     AISTTLTATVPFANPLPESGVPHDVFATGFGPRLLAIIGGAVGSTILIVLALVSVFRF
                     WRTNRRIVWGNLLIVAGTFAAASGGTGLALGESSAFAVSLLVAVTLIWAGYRVTSGGR
                     RLTRSPDMSTVTAQPPM"
BASE COUNT          262 a          638 c          591 g          311 t
ORIGIN      
        1 agaccaccgc gctgcgggtg atagcgggat tcgagaacgc cggcgggagc atcgagatcc
       61 acggccggaa ggtcctcgac gacggggtct tcgtcccacc cgagaagcga agggtgggca
      121 tggtgttcca ggactacgcc ctcttccccc acatgaccgt tgcctccaac gtcgcctacg
      181 gcctccccag gggcgagcga gagccgcggg tcggtgaggt gctggagctg gtcgggctca
      241 ccgggctcga ggatcggatg cccaacgagc tgtccggcgg ccagcagcag agggtggccc
      301 tggccagagc gctggccccc gagccggagg tcatcctcct cgacgagccc ttctccaacc
      361 tcgacgccag tctgcgggac cgggtgagac gtgagttgcg ggtgatcctc accgaggctc
      421 ggaccactgc ggtgttcgtg acccacgacc aggaagaggc tcttgccacc tctgacatcg
      481 tggcggtgat gaaggacggg cgcgtcctcc aggcggacac ccccgccgac ctctactcca
      541 acccggccga cccctggatc gccgagttcc tgggcgatgc cgacgtcttg cattccgagg
      601 cccgaaacgg cagggcagac accccggtcg gctcgttcca aaccgacctc accgggaagg
      661 tgtcggtgat catcagaccg gagaacgtgc agatctcgct gggtgagacc cccaatgcgg
      721 tggtggcggg gtctgagttc ttcggacacg accagctggt gacggtcgca ctccacgggg
      781 ggacccgggt ccgagcccgc atcggccccc gcccgatcta ccgagtcggc gacgcgtgcc
      841 gggttcgggc catcgatgcc cggatcttcg ccgtctgacc cgccgtaatt gggatcgtca
      901 ggggtcagat gcggacctgg gcaacccaat cacccctagc ctggctcggg atggactcgt
      961 tgctcgccct gctcgccgcg ttggctgccg ccgccttcgc cgccgacctc tggctcgact
     1021 accggaaacg tccacgtcct cacatcgcgg cctacgccac cgggatgtcc atgttcgccg
     1081 tcgccaccct ggctttcttc atcgggctcg cggcgggctg gaccggtgtc gtgtaccgga
     1141 ccttctacct gttcggtgct gtgctcaaca tcccgtttct cgccctgggg tcgatgttcc
     1201 tcgtggtggg gaagcgctcg ggccacgcga tggccatcgc cctgggagcg ctcgccgcga
     1261 tctcgaccac gctgaccgcg accgtcccct tcgccaaccc gctccccgag tccggggtgc
     1321 cccacgacgt gttcgccacc gggttcgggc cgagactctt agccatcatc ggcggcgccg
     1381 tcggatcgac catcctcatc gtcctcgccc tggtcagcgt tttccgattc tggaggacga
     1441 accggcgcat cgtatggggc aacctgctca tcgtggccgg gaccttcgcc gcggcgtccg
     1501 gtggcaccgg gctcgccctc ggggagagca gcgcgttcgc cgtctccctg ttggtggcgg
     1561 tgaccctgat ctgggccggg tatcgggtga cgtcgggcgg gcggcgcctg acccgctccc
     1621 cggatatgag cacggtgacc gctcagcccc cgatgtagct catctccacc cgtgagccga
     1681 tgctgaccgt ctgcgaggac cggatctccg agtagcgatc gtcccgggtc gaccacacac
     1741 ggtcgactat ctcgcccacg tcgtgcccgg cgcggatcgc atcacggaga ctggtgccgg
     1801 ac
//
LOCUS       DATITW010000287         1562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_14368, whole genome shotgun sequence.
ACCESSION   DATITW010000287 DATITW010000000
VERSION     DATITW010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14368"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..625)
                     /locus_tag="VLS86_04090"
     CDS             complement(26..625)
                     /locus_tag="VLS86_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSO49703.1"
                     /translation="MIRVVLAEDQAMVRGAFAGLLDLEPDIEVVAVAEDGEQGLAAVT
                     EHEPDILLTDIEMPGMTGLELASRLKDHPTVRVVIVTTFARSGYLRRALDAGVSGYVL
                     KDSPIEDLADTLRRVAAGYRVIAPELAVSAWTGSDPLTDREREVLREVGEGHPNGEIA
                     LRLHLAEGTVRNYLSSAIVKLGARNRTEAFAKAQQMGYL"
     gene            complement(622..>1562)
                     /locus_tag="VLS86_04095"
     CDS             complement(622..>1562)
                     /locus_tag="VLS86_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HSO49704.1"
                     /translation="FVVSEIRGWDIGGATVLMLLALATAPFDSTAIMALPIYAAALVA
                     RSASQETLRLRLSVITALTLTGFFLSSIPWPFRVFIFIPVIMIWVIGSAVFHDVSRDH
                     HAVTLQAENRRVAHLATIAERERIARDVHDIAGQALTAIIVRSQLVQRLARSDQRRAT
                     EEADEVERIARDALSSIRESVAGWHQASLLDELAVARDALAAAGTELETLGDFEVDLA
                     PSVENVLALGLREAVTNVLRHANASTVEARIDSQDGGVSLTVTDDGIGPTKGTGSGIQ
                     GMRERVIAAGGTLTFGPGVTGGTELRIDMPFGGSSS"
BASE COUNT          275 a          475 c          499 g          313 t
ORIGIN      
        1 gttggccggc tggtgttcga gggtgttaca ggtatcccat ttgctgggcc ttggcgaatg
       61 cctcggtacg gtttcgggcc ccgagcttga cgatggcgga tgagaggtag ttgcgtacgg
      121 ttccttcggc gaggtggagc cttagcgcga tctccccgtt gggatgacct tcaccgacct
      181 ctcgcaggac ttctcgctcc cggtcagtga gtgggtcgct gccggtccat gcggacactg
      241 cgagctccgg ggcgattacg cgatagcctg ctgccacccg cctgagagtg tcggctagat
      301 cttcgatggg tgagtctttg aggacatatc cagacacccc ggcatcgagc gcccgccgca
      361 gatagccgct tcgggcgaag gtcgtcacga tcacgactcg gaccgtcgga tggtccttga
      421 gacgagaagc cagctcgagt cccgtcattc ctggcatttc gatgtcggtg agcaggatgt
      481 ccggctcatg ctctgtgaca gctgccaaac cctgctcgcc atcttcggcg acggcgacta
      541 cctcgatgtc cggttcgagg tcgaggagtc ccgcaaaggc tcctcgcacc atggcctggt
      601 cttcggcgag gacgacgcgg atcatgaaga acttccaccg aatggcatgt cgatccgtag
      661 ctctgtcccg cctgtcactc cgggtccgaa cgtgagggtt ccccctgcgg cgatcacacg
      721 ttcgcgcatg ccctggattc ccgagccggt tcccttggtg ggtccgatcc cgtcgtcggt
      781 caccgttagt gatactcccc cgtcctggct gtcgattcgg gcctccaccg tgctcgcgtt
      841 ggcgtggcgc agaacattgg tgaccgcctc gcgaagtccc aaggccagaa cgttctcgac
      901 cgaaggtgcc agatcgacct caaagtctcc gagcgtctcc aactcggtgc ctgccgctgc
      961 gagggcatcg cgggcaacgg cgagctcgtc gagcagcgac gcctgatgcc atccagcaac
     1021 gctttccctg atcgaagaca gtgcatcgcg agctatgcgt tccacctcat cggcttcctc
     1081 tgtggcgcga cgctggtccg acctggccag tctttggacg agttggctac gcacgatgat
     1141 ggcggtgagg gcctggcctg cgatgtcgtg gacgtcgcga gcgatccgtt cccgctcggc
     1201 gatggtggcg agatgagcga ctcggcggtt ctcagcctgg agcgtgaccg cgtgatggtc
     1261 gcgcgacaca tcatggaaga cggcggatcc aatgacccag atcatgatca ccgggatgaa
     1321 tatgaagacc cgaaacggcc aagggatcga actcaggaag aagccggtca gagtgagggc
     1381 ggtgatcacc gacagtctga gtcggagcgt ctcctgactg gctgacctgg caacgagagc
     1441 ggcggcgtag atgggcagcg ccatgattgc tgtcgaatcg aagggtgctg tggcgagtgc
     1501 cagcagcatc aggacagttg ctcccccgat atcccaacct cgtatctccg atacgacaaa
     1561 gg
//
LOCUS       DATITW010000288         2538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_134216, whole genome shotgun sequence.
ACCESSION   DATITW010000288 DATITW010000000
VERSION     DATITW010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134216"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VLS86_04100"
     CDS             complement(<1..344)
                     /locus_tag="VLS86_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSO49705.1"
                     /translation="MADQADFERDAMQYTRQLYSAAMRMTRNPSDAEDLVQETFLKAY
                     RAYHTFEEGTNLKAWLYRILTNTYINKYRKDTRRPSEVDLGSVEDLYLYRRIGSEESA
                     EAARTTEDRVLEG"
     gene            complement(426..497)
                     /locus_tag="VLS86_04105"
     tRNA            complement(426..497)
                     /locus_tag="VLS86_04105"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(463..465),aa:Thr,seq:cgt)
     gene            complement(579..1616)
                     /locus_tag="VLS86_04110"
     CDS             complement(579..1616)
                     /locus_tag="VLS86_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49706.1"
                     /translation="MSGPFHGVIGHERVIELLVRELSGPAQAYLFTGPDSVGKGTLAA
                     RFAAALLCEQQGEHDVECRSCRLVRTMVHPDVTVIEPEGATSLGVDQARAVVSRASLR
                     PVESDKSIFLIPEAGMMTDQAANALLKTLEEPTASVIFLLVAESEDDFPSTVASRCRT
                     IHVGRVPMDVMVAALEERGMATLEAEGVAAVSGGRPGLALALMDRPEVSRFRDLWLSI
                     PGQVTPRPGDGHRLASSVLAEITPLVDSSVREDLPKERLQRARRRSEQALLVNGLEIL
                     ASWYTDSASLQMGGPVRNTDLPLGSFTDVTPRKAVMSAELILDAVVDIQANLRRELVL
                     ANLFAGLGSEN"
     gene            complement(1613..2227)
                     /gene="tmk"
                     /locus_tag="VLS86_04115"
     CDS             complement(1613..2227)
                     /gene="tmk"
                     /locus_tag="VLS86_04115"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002858064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HSO49707.1"
                     /translation="MAANGSSNHRYNALEGGDGSGKTTLSAALATRLRTAGEEVVEVR
                     EPGGTPLGESVRELLLDSEHVDPWAEVFLFAAQRAQLAHDVVAPALERGAWVISDRTY
                     YSSIAYQGRARGLGEDTVRRINETGLGGVVPGRVFVIEVDAGTALDRQHRPDRIGREG
                     VDFQEAVRSAYRDLAASEPGRVILLDGRDTTDDLVDRVMAALGR"
     gene            2308..>2538
                     /locus_tag="VLS86_04120"
     CDS             2308..>2538
                     /locus_tag="VLS86_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HSO49708.1"
                     /translation="MPKSLVIVESPTKARTISAFLGSEVAVESSMGHIRDLPRSAAEI
                     PARAKGETWARLGVNVDAGFEPLYVVPKERRER"
BASE COUNT          426 a          854 c          835 g          423 t
ORIGIN      
        1 ccttccagga ccctgtcctc ggtggtgcgg gccgcctccg ccgattcctc ggacccgatc
       61 ctccggtaga gatagaggtc ctcgaccgac ccgaggtcca cctctgaggg ccggcgagtg
      121 tccttccggt acttgttgat gtaggtgttg gtcaggatcc ggtacagcca ggccttgagg
      181 ttggtccctt cctcgaaggt gtggtaggcc cgatatgcct tgagaaacgt ctcctggacc
      241 aggtcctctg cgtcggaggg attgcgggtc atgcgcatgg cggcgctgta cagctgccgt
      301 gtgtactgca tcgcgtcgcg ttcgaagtcg gcctggtccg ccatcggcaa tgacggtacc
      361 ggagggggac cgaaacccta agtctggccc cactcgaagc cagggatcga gttgggagat
      421 gtcggagccg gagaggggag tcgaacccct ggcctattca ttacgagtga attgctctac
      481 cactgagcta ctccggcgcg agcacaatgg tatcggtggc gccagtgcag ggccagacag
      541 tggccgctct ggtgaggtgg cggcaggcgg tgggtgagtc agttctccga gccgagaccg
      601 gcgaacagat tggccaggac cagctcccgc cgcaggttgg cctggatgtc caccacggcg
      661 tcgagaatca gctctgcgga catcaccgcc ttgcgcgggg tgacgtcggt gaacgagccg
      721 agggggaggt cggtgttgcg gaccgggcca cccatctgca gcgaggccga gtcggtgtac
      781 caggacgcca ggatctcgag accgttgaca agcagcgcct gctccgaccg gcgacgggct
      841 cgttgcagac gctccttggg caggtcctcc cgcaccgagc tgtccacgag cggggtgatc
      901 tcagccagca cactcgaggc cagtcggtgc ccgtctcccg gccgcggagt cacctgaccc
      961 ggtatcgaaa gccagaggtc gcggaacctc gacacctccg gtcggtccat cagggcaagg
     1021 gcgagcccgg gccggccgcc tgacaccgcg gccacgccct ccgcctccaa tgtggccatc
     1081 cccctctcct ccagggcggc caccatgaca tccatcggca cccgtccgac gtggatggtc
     1141 cggcatcggg atgccaccgt ggacgggaag tcatcctcgg actccgccac gaggaggaag
     1201 atcaccgacg cggtgggctc ttcgagcgtc ttgagcaggg cgttggcggc ttggtcggtc
     1261 atcatcccgg cctcgggaat gaggaagatc gacttgtcgg actcgacggg tcggagcgaa
     1321 gccctgctga cgacggcccg ggcctggtcg acgccgagac tggtcgcccc ctcgggctcg
     1381 atcaccgtga cgtcgggatg gaccattgtc cgcaccagac gacaggatct gcattcgaca
     1441 tcgtgctcac cctgctgctc gcagagcagg gcggcggcga agcgggcggc gagggtcccc
     1501 ttccccaccg aatcgggccc ggtgaacaga tatgcctgcg ccgggcccga aagctctcga
     1561 accaggagct cgatcactcg ctcgtgaccg atgaccccgt gaaatggtcc gctcatcgac
     1621 ccagggccgc catgacccga tccacgaggt cgtcagtcgt gtcccggccg tcgaggagga
     1681 tcaccctccc tggctcggag gcggccaggt ctcgatacgc gctcctcacc gcctcctgga
     1741 agtcgacgcc ctcgcggccg atccgatccg ggcgatgctg acggtccagg gccgtgccgg
     1801 catcgacctc gatcacgaac acccggcccg ggaccacccc gcccaggccg gtctcgttga
     1861 tccggcgcac ggtgtcctcc ccgagccccc gggcgcggcc ctggtaggca atcgacgagt
     1921 aataggtccg gtcgctgatg acccacgcgc cacgctccag cgccggggcg accacgtcgt
     1981 gggcgagttg ggctcgttgg gccgcgaaca agaacacctc ggcccacgga tcgacatgct
     2041 cggaatcgag caggagctct ctgacgctct ccccgagggg ggtgccgccc ggctccctca
     2101 cttcgactac ctcctcgccg gcggttcgaa gtcgggtggc cagagcagct gacaacgtcg
     2161 tcttgccgct cccatcgccg ccttccagcg cattatatcg gtgattacta gagccgttgg
     2221 ctgccactcg aggcgaacgg taacggcccg cagagatacg agtcatattg acataaggcg
     2281 gttccatgcg gtcaactcct caggctgatg cctaagagcc tcgtcattgt cgagtccccg
     2341 accaaggcca ggaccatctc cgcgtttctc ggctcggagg tggcggtcga gtcttcgatg
     2401 ggacacatcc gcgaccttcc ccggtcggca gcggagatac cagcgcgcgc caagggagag
     2461 acctgggccc ggctcggcgt caacgtcgac gccggcttcg agcccctcta cgtcgtgccc
     2521 aaggaacgcc gggaacgg
//
LOCUS       DATITW010000289         1540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_414970, whole genome shotgun sequence.
ACCESSION   DATITW010000289 DATITW010000000
VERSION     DATITW010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414970"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VLS86_04125"
     CDS             complement(<1..500)
                     /locus_tag="VLS86_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSO49709.1"
                     /translation="MKPELLIFDVNETLLDLSPLKIDFEDVFGKSAPMGEWFGRMLHG
                     ALVANQLDEYRAFGVIGVEALLLVAERHGVDLDEATATEVVTRLAILPAHLDVIPALE
                     RLLDAGFRTAALTNGSTKAANVQIENAGLHTFIQRVISVEEVGRFKPDPLVYHHAAKV
                     MGVEIE"
     gene            complement(497..>1540)
                     /locus_tag="VLS86_04130"
     CDS             complement(497..>1540)
                     /locus_tag="VLS86_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSO49710.1"
                     /translation="EFHKITGLRSYAGDDLMLELPWPEHSRFPNWGGVIRRRDLDGQV
                     ATLAGKQGVQELEKTVANPVIEDGLLTSVTLEHDGQRAEVTPDITIIADGSMNRFGRD
                     LGSERRRDYPMGLAARGYYTSPRSTDPFLESQLDLRDSSGATMPGYGWVFPLGDGTVN
                     VGVGLLSTFKRWKHVNTPKMLADYVATAPDYWELSEESKLTDPLGGKLTMSFSKGPLV
                     GANWITIGDAAGAINPWNGEGISYAYETGRIAADHVGQALGTGDFSLLRRYPQYLEDE
                     YGLYYKVARIFVKLIGNPTVMRTLAHTGLRNRTLMEWTLKVMANLLDEDDKHMGERTY
                     DALAAIVRTLPTK"
BASE COUNT          269 a          483 c          489 g          299 t
ORIGIN      
        1 tcctcgatct cgacccccat caccttggct gcgtggtggt acaccagggg gtcgggcttg
       61 aagcgaccga cttcctcgac agagatcacc cgttggatga aggtgtgcag gccggcattc
      121 tcgatctgga cattggccgc cttggtgctg ccgttggtca gggcggcggt gcgaaagccg
      181 gcgtcgagca gacgctccag ggccgggatc acatcgaggt gggccgggag gatggcgaga
      241 cgggtgacca cctcggtggc ggtcgcctcg tcgaggtcga ccccgtgtct ctcggcgacc
      301 aggagcaggg cttcgacccc gatgacgcca aaggctcgat actcgtcgag ttgattggca
      361 accagggccc cgtgcagcat tcgcccaaac cattcgccca tgggcgcaga cttgccgaac
      421 acgtcctcga agtcgatctt cagcggcgat aagtcgagaa gcgtctcatt cacatcgaat
      481 atgagaagct cgggtttcac ttcgtgggaa gcgttcggac gatcgcggcc agcgcgtcgt
      541 aggtgcgctc gcccatgtgc ttgtcatcct cgtcgagcag attcgccatg accttcaatg
      601 tccactccat cagtgtgcgg ttgcgcagac cggtgtgggc cagagtgcgc atgaccgtcg
      661 ggtttccgat cagcttcacg aagatccggg ccaccttgta gtagagcccg tactcatcct
      721 cgagatactg tgggtatcgg cgcaacaggc tgaaatctcc agtgccaagt gcctgcccga
      781 cgtgatcggc ggcgatgcgc ccggtctcgt aggcgtagga gatcccctcg ccattccacg
      841 ggttgatcgc acctgccgcg tcaccgatcg ttatccagtt ggccccgacc agagggccct
      901 tggagaagga catggtcaac ttgccaccaa gggggtcggt cagcttcgac tcctcgctca
      961 actcccagta gtcgggagcc gtggccacgt aatcggccag catcttcggg gtattgacat
     1021 gcttccaccg cttgaaggtg gagagaaggc cgacaccgac gttgaccgtc ccgtccccca
     1081 gcgggaagac ccacccgtaa ccaggcatgg tggcccccga ggagtccctg aggtcgagtt
     1141 ggctctccag gaacggatcg gtggagcgcg gtgaggtgta gtagcccctg gcagccagac
     1201 ccatgggata gtccctgcgt ctctcgctgc ccaggtcgcg cccgaagcgg ttcatcgagc
     1261 cgtcggcgat gatcgtgatg tccggtgtta cctcggctct ctgcccgtcg tgctccaggg
     1321 tcacgctggt gaggagaccg tcctcgatca ccgggttcgc caccgtcttc tccagttcct
     1381 gcaccccttg cttcccggcg agggtggcca cctggccatc caggtcgcgt ctgcggatca
     1441 caccgcccca gttggggaag cgggagtgct cgggccaggg caattcgagc atcagatcgt
     1501 cgccggcata agagcgcaga ccggtgatct tgtggaactc
//
LOCUS       DATITW010000290         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_214750, whole genome shotgun sequence.
ACCESSION   DATITW010000290 DATITW010000000
VERSION     DATITW010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214750"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VLS86_04135"
     CDS             <1..631
                     /locus_tag="VLS86_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017992313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HSO49711.1"
                     /translation="SPLPAVYTGERMKAYREWLPADRYEAVGSIGGSFVSDDIEDYYL
                     TPHDLGYGFYIKFDHEFIGGDALEAMAEQPHRKKVTFEWNPDDVVRVWQSLFEPEGQN
                     FKHIDLPLSNYTSASYDAVLRDGETVGFSMFSGYTYTDRAFLSLGTVDPDVEVGDELT
                     LVWGEADGGTRKPTVERHRQTEIQVRVAPTPYSRDARESYGGRWRGRAS"
     gene            complement(670..1746)
                     /locus_tag="VLS86_04140"
     CDS             complement(670..1746)
                     /locus_tag="VLS86_04140"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HSO49712.1"
                     /translation="MISDLPGIRCMLMRGGTSKGAFFVAGDLPSGPAERDALLLRVMG
                     SPDPRQIDGVGGAHPLTTKVAVVTPSESADADLEYLFLQVAVDRPEVTDRQNCGNMLA
                     AVGPFAVERGMIAPTEGRAAARILMRNTEGMALVTFPVTGGSPVYAGETEIAGVPGSA
                     AEIRIEFEDIAGSSTGALLPTGRISDEIDGIETTLIDNGMPVVVMRAADLGVTGYEPC
                     EELEANSELRETLEGIRLAAGALMNLGDVTTTTVPKLTLVSPPREGGALSTRTFIPHR
                     CHHAIGVLGAVSVATAALLPGSSASSVARIRSGDDVVRLEHPTGSFDALVRLATDGEP
                     RVLGAGIVRTARKLMDGLVFPRTY"
     gene            1799..>2065
                     /locus_tag="VLS86_04145"
     CDS             1799..>2065
                     /locus_tag="VLS86_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSO49713.1"
                     /translation="MSRESILVVSAHVADFVWRAGGAMALYAARGDEVTVVCLSYGER
                     GESARYWRQGLTLEQVKEARHREGAAAAKGIGANFVAFDGDDYPL"
BASE COUNT          357 a          674 c          707 g          327 t
ORIGIN      
        1 ctcaccgctc cctgcggttt acaccgggga gaggatgaag gcgtaccggg agtggttgcc
       61 ggcggaccgg tacgaggcgg tcggctccat cggtggcagc ttcgtctcgg acgacatcga
      121 ggactactac ctaacgcctc atgacctcgg ttacggcttc tacatcaagt tcgaccacga
      181 gttcatcggg ggtgacgccc tggaggcgat ggccgagcag ccacaccgca agaaggtcac
      241 cttcgagtgg aaccccgacg acgtggtccg ggtctggcag tccctgttcg agcccgaggg
      301 ccagaacttc aagcacatcg acctgccgct gtccaactac acctcggcga gctacgacgc
      361 tgtattgcgg gacggcgaaa cggtcggctt ctccatgttc agcggctaca cctacaccga
      421 tcgggccttt ctctcgctgg gcaccgtcga tcccgacgtc gaagtgggag acgagttgac
      481 cctggtatgg ggtgaagccg acggagggac ccggaagccc actgtcgagc gacacaggca
      541 aaccgagatc caggtccggg tcgcccccac cccgtactct cgggacgccc gcgaatccta
      601 cgggggcagg tggcggggaa gggcgagctg agccgactcc ggcgccagag gaggaggccc
      661 ggagcgggct cagtacgtcc ggggaaagac cagtccgtcc atcagcttgc gggcggtgcg
      721 gacgatcccg gccccgagca cccgcggctc cccgtccgtg gccagccgga ccagggcgtc
      781 gaagctaccc gtcggatgct cgagacggac gacatcgtcg cccgacctga tccgggcgac
      841 cgaggaggcg gaggagccgg gaagaagggc tgcagtggcg acgctcaccg ccccaaggac
      901 acctatcgca tgatgacatc gatgggggat aaaggtccgg gtggagagtg ccccgccctc
      961 tcggggcggg gagaccagcg tcagtttggg gacggtggtc gtggtgacgt cgccgaggtt
     1021 catcagcgca cctgccgcga gccggatccc ctccagggtc tctctcagct ccgaattggc
     1081 ctcgagttcc tcacagggct cgtacccggt gacaccgagg tcggccgctc tcatcaccac
     1141 caccggcatc ccgttgtcga tgagtgtggt ttcgatgccg tcgatctcgt cgctgatacg
     1201 acccgtgggg agcaaggcgc cggtgctcga gccggcgatg tcctcgaact cgatcctgat
     1261 ctcggccgcg ctcccgggca cgccggcgat ctcggtctcc ccggcataga cggggctacc
     1321 tcccgtaacc gggaaggtga ccaacgccat cccctcggtg ttccgcatca ggatgcgagc
     1381 cgcggcccga ccctcggtgg gggcgatcat cccccgctcc acggcgaacg ggcctaccgc
     1441 cgcaagcatg ttcccgcagt tctgccgatc ggtgacctca ggccggtcca ccgccacctg
     1501 aaggaacaag tactcgaggt cggcgtccgc actttcggag ggcgtcacca cggccacttt
     1561 tgtggtgagg ggatgggcgc cgcccacccc gtcgatctgg cgcgggtcgg gcgagcccat
     1621 gacccggaga agcagggcgt ctcgctccgc ggggccagag ggcaggtcgc cggcgacaaa
     1681 gaacgccccc ttcgaggtgc cgcctctcat cagcatgcaa cggatacctg gaaggtcgct
     1741 gatcatcgtg ttctccttcg ggaagatagt gagaaaggcc cgggataggg ttggggacat
     1801 gagccgggag tcgattctgg tggtcagtgc ccacgtcgcc gacttcgtgt ggcgcgccgg
     1861 tggcgccatg gccctgtacg ccgcgagggg cgacgaggtg accgtggtct gcctctccta
     1921 cggggaacga ggtgagtccg cacggtactg gcgacagggg ttgaccctgg agcaggtcaa
     1981 ggaggcgaga caccgggagg gtgctgccgc cgccaagggc atcggcgcca acttcgtcgc
     2041 cttcgacggc gacgactacc cgctg
//
LOCUS       DATITW010000291         1679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_655161, whole genome shotgun sequence.
ACCESSION   DATITW010000291 DATITW010000000
VERSION     DATITW010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655161"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="VLS86_04150"
     CDS             complement(<1..1117)
                     /locus_tag="VLS86_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49714.1"
                     /translation="MGTVWQIASREIRERGRSKAYLLTTLLTLLLVVGLVVVPSLIGG
                     GTSEYTVGSVGEGNEPIISAAELLGNATDEPGDEPSVSIESESFPDRETAEAALEEGD
                     LDAVLVNGEEAVLESVGGFGESTVLSLLQQGAASVELEAIVSESGDAAADVIEVMTSD
                     PLETTTLSGQEAGDASRGAVAYAGLLLLYMAVLLYGTWILSGVTEEKSNRVVEVLLSS
                     IKPWQLLAGKIIGIGVLGIAQFAGTIAVALVALEVTGAFELPSLDAGAVANLIVWFIL
                     GFLLFAVMFAAAGSLVSRIEDAQTVALPMSLTAVVGFFVSIAALSDPDGLAAVIGTFI
                     PFTAPFVVPVRAALDAIPVWQYLTAVVLTIATIVGLVF"
     gene            complement(1123..>1679)
                     /locus_tag="VLS86_04155"
     CDS             complement(1123..>1679)
                     /locus_tag="VLS86_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO49715.1"
                     /translation="RWLERFGLSDRRMDPVEALSHGNQQRVQLAAALVHDPELLVLDE
                     PFSGLDPIASETMSDILREQAAAGRTVVFSSHQLDLVEDLCEEVAIINSGAIVVEGNV
                     RQLKDAAPMRHLELEVDGDVGNLLDSLEGISEVRSDGPRTTAVVDATTDVRGFLARAQ
                     EAGRLRHFSYTTPSLSDLFREAVR"
BASE COUNT          312 a          571 c          543 g          253 t
ORIGIN      
        1 cgaagaccag cccgacgatc gtggcaatgg tcagcacgac cgcggtcagg tattgccaca
       61 ccggaatcgc gtcaagggcg gctcggaccg ggaccacgaa cggcgcggtg aaggggatga
      121 aggtcccgat cactgcggca agcccgtcgg ggtcggagag ggcggcgatg gagacgaaga
      181 aaccgacgac ggcggtcaac gacatgggca gggccacggt ctgcgcgtcc tctatgcggc
      241 tcaccagaga tccggcggca gcgaacatca ccgcgaacag caggaatccg aggatgaacc
      301 acacgatgag attggccacg gcgccggcat cgagactggg aagctcgaac gcccccgtca
      361 cctcgagcgc caccagcgca acagcgatgg tgcctgcgaa ctgggcgatg ccgaggacgc
      421 cgatcccgat gatcttgcct gccaggagct gccagggctt gatgctggag agcaggacct
      481 ccaccacacg attcgacttc tcctcggtca cgccggagag gatccaggtc ccgtagagca
      541 ggaccgccat gtagagcagc aagagcccgg cgtaggcgac ggcgccccgg ctcgcatccc
      601 ctgcctcctg accggagagc gtggtggtct ccagggggtc cgatgtcatc acctcgatca
      661 catcggccgc ggcatcgccc gattcggaga cgatggcctc cagctccacc gaggccgcgc
      721 cctgctgcaa gaggctgagg acggtcgact cgccaaaacc gccgacgctc tccaacacgg
      781 cctcttcacc attcaccagg acggcatcga ggtcgccttc ctccagcgcc gcctccgccg
      841 tctcgcggtc ggggaacgac tcggattcga tcgagaccga tggctcgtca cccggttcgt
      901 ccgtcgcatt ccccaacagc tcggcagcgg agatgatcgg ctcgttgcct tcgccgacac
      961 tccccacggt gtactcggac gtcccaccgc cgatcagact ggggaccacc accaggccca
     1021 ccaccaggag cagggtgagg agggtcgtga gcagataggc cttggatctc cctcgctctc
     1081 gtatctcccg agaggcgatc tgccacaccg tccccattgc gctcatctca cggcctcgcg
     1141 gaagaggtcg gagagcgacg gtgtggtgta gctgaagtgt cggagccggc cggcctcctg
     1201 ggcccgggcc aggaaccctc tgacgtcggt ggttgcgtcg acaacggccg tggtccgggg
     1261 cccgtcggag cggacctcgc tgatgccttc cagcgagtcg aggaggttcc cgacgtcgcc
     1321 gtcgacctcc agctccaggt gacgcatcgg agcggcatcc ttcagctggc gcacattgcc
     1381 ctcgaccacg atcgccccgg aattgatgat cgccacctcc tcgcacagat cctcgacgag
     1441 gtcgagctgg tgggaggaga aaacgacggt cctgccggcc gcggcttgct ctctgagaat
     1501 gtcggacatg gtctccgagg cgatgggatc gagcccgctg aacggctcgt ccagaaccag
     1561 cagctcgggg tcgtggacca gggccgccgc cagctgcacc cgttgctggt tgccgtggga
     1621 cagggcctcc acagggtcca ttcgcctatc ggagaggcca aaccgctcca gccacctgt
//
LOCUS       DATITW010000292         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_134344, whole genome shotgun sequence.
ACCESSION   DATITW010000292 DATITW010000000
VERSION     DATITW010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134344"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2006)
                     /locus_tag="VLS86_04160"
     CDS             complement(<1..>2006)
                     /locus_tag="VLS86_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HSO49716.1"
                     /translation="IVRGRSDQLIFGTLILARQEVDAVSYGFAETQPRAISLRYQPDG
                     WPVELNVLSTHPLAPTTTERAGLRDAQLEFAGDWASEQEGAFFVVGDLNASPWSWPFR
                     SLVSEGGLRNSQIGFGLQPTFPATSVVLLRVPIDHLLHSEELRVRDRRLGAALGSDHF
                     SLIVDLEHVGEPPPHLREGDLRSSVPPMSDRFTDRRRRLLELIGEDGLAVVPAAVEVI
                     RSNDTSFEFHQDPDFFYLTGFPEPEAVAVLTPGHDDAEYTLFVRPRDPEMEAWNGYRV
                     GTDGAVERFGADKAYDLDQIDDVLTRYMIGREVLWYRSGNPHHDDRISGIISRARSHR
                     ERFGEAVPSTVRDLSVSLSEMRLIKTDEEIDSLRKACELSARGHAEAMRFAAPGLYEY
                     QIQAALEYPWRQAGARHNGYQSIVASGANACVLHYVENDRMVESGDLILIDAAAEVDG
                     YSSDITRTFPADGRYTGPQRAIYEVVHAAFEKGLELSSPGSSLRGIHDAATRVLTEGM
                     VELGLLPRSVEESLAMHHYNHFFFHGTGHWLGLDVHDMGSYRVDREPRKLEPGMAFTV
                     EPGLYIAPSKAEITLTMLEYDQDEWNQRRILEGRAAAVAQETAARDAAEKVIHTVPPE
                     YLGIGVRIEDDVVITPSGHDNLTSMLPSRLDEVEALAAERSLLP"
BASE COUNT          347 a          639 c          678 g          342 t
ORIGIN      
        1 atcgggagca gcgaccgctc cgcggcgagg gcttcgacct cgtcgagccg ggaggggagc
       61 atcgaggtca ggttgtcgtg gccggaaggg gtgatgacca cgtcatcttc gatcctgacc
      121 ccgattccga gatactccgg aggcaccgtg tggatgacct tctccgccgc gtcgcgggca
      181 gcggtctcct gcgccacggc cgccgccctt ccctcgagga tgcgacgctg gttccactcg
      241 tcctggtcgt attcgagcat ggtgagggtg atctcggcct tgctcggcgc gatgtagagg
      301 ccaggttcca cggtgaaagc cattcccggc tccagcttcc ggggctcacg atcgacccgg
      361 tagctgccca tgtcgtgaac gtcgagaccc agccagtgcc cggttccatg gaagaagaag
      421 tggttgtagt ggtgcattgc cagggattcc tccaccgatc gggggagaag ccccagctcc
      481 accattccct cggtgaggac gcgggtggcg gcgtcgtgga tccccctgag gctggatccc
      541 ggcgaggaca actcgagacc cttctcgaag gcggcatgca ccacctcgta gatcgcccgt
      601 tgcggtcccg tgtacctgcc gtcggcgggg aacgtcctgg tgatgtcgga ggagtacccg
      661 tccacctcgg cggccgcatc gatgaggatg agatcgccgc tctcgaccat ccggtcgttc
      721 tcgacgtagt gcaggacgca ggcgttagcc cctgaggcga ctatcgactg gtacccgttg
      781 tgtcgggccc cggcctggcg ccacgggtac tcgagcgcag cctggatctg gtactcgtag
      841 agaccgggtg cggcgaagcg catcgcctct gcatgcccac gtgccgacag ctcgcaggcc
      901 ttgcgcaggc tgtctatctc ctcgtctgtc ttgatgagac gcatctcgga cagggaaaca
      961 gagaggtcgc gcaccgtcga ggggacggcc tcaccgaagc gctcgcggtg acttcgggcc
     1021 ctggagatga tcccgctgat ccggtcgtcg tggtgggggt tgccgctgcg ataccagagg
     1081 acctcgcgac cgatcatata ccgggtgagg acgtcgtcga tctggtcgag gtcatatgcc
     1141 ttgtccgccc cgaatcgctc gacggcccca tcggtgccga cccggtagcc attccacgcc
     1201 tccatctcgg ggtcccgggg acggacgaac aaggtgtact ccgcgtcgtc gtgaccgggg
     1261 gtgaggacgg cgaccgcctc cggctcgggg aaaccggtga gatagaagaa gtcgggatcc
     1321 tgatggaact cgaagctcgt gtcgttgctg cggatgacct cgactgcggc aggaaccaca
     1381 gcgagcccgt cttcgccgat cagctcgagg agacggcggc gacggtcggt gaagcgatct
     1441 gacatggggg gaacgctact tcgcaagtca ccctcccgta agtggggtgg gggctccccg
     1501 acatgctcca ggtcgacgat cagcgagaaa tgatccgagc cgagggccgc tcccaacctg
     1561 cggtcccgaa cccgcagctc ttcgctgtgc aacagatgat cgatcgggac ccgcagcagc
     1621 acgaccgaag tcgcgggaaa tgtcggctgg aggccaaagc cgatctggga gttcctgagc
     1681 ccgccctcgg agaccaggga tcgaaacggc cacgaccacg gcgaagcgtt cagatctccg
     1741 accacgaaga aagctccctc ctgctcactg gcccaatctc cggcgaactc gagctgggcg
     1801 tcgcgaagac cggcgcgctc ggtggtcgtc ggcgccaggg ggtgagtgga gagcacgttg
     1861 agctcgaccg gccagccgtc cggctgatac cgaagcgaga tcgcccgtgg ctgggtctcg
     1921 gcgaagccgt aggagacggc gtcgacctcc tggcgcgcca ggatcagggt cccgaagatc
     1981 agctggtccg accgccccct gacgat
//
LOCUS       DATITW010000293         1549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_254612, whole genome shotgun sequence.
ACCESSION   DATITW010000293 DATITW010000000
VERSION     DATITW010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254612"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VLS86_04165"
     CDS             <1..200
                     /locus_tag="VLS86_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49717.1"
                     /translation="ARQEGLPANLVMGGTIEYSKLNCDTIDLDLRRAGQDRFEALRDI
                     QRECYDDATARGVSTAKARLR"
     gene            309..1052
                     /locus_tag="VLS86_04170"
     CDS             309..1052
                     /locus_tag="VLS86_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSO49718.1"
                     /translation="MEVLGIDIGGTGIKVARVDVRTGKLTTELIRYPTPDPSTPKKVL
                     LVVRKAIREFAYEGAVGCGFPGVVRNGVIQTAANVSKKWIDVRADELYREATGLPFFL
                     ANDADLAGVAEMRFGAGRGRKGLVIMLTFGTGIGTGVFMDGKLVPNSELGHLEVRGHE
                     AEKWASERTRIVKKLSFRDWAWRVNVYLNRLHAYFWPELFIIGGGVSREFEKFRKYLE
                     VPCELLPATLRNRAGVIGAAYAASRKLRI"
BASE COUNT          303 a          479 c          519 g          248 t
ORIGIN      
        1 gagccaggca ggagggtctg cccgcgaatc tggtgatggg cggcaccatc gagtactcca
       61 agttgaattg cgacaccatc gacctcgatt tgcgtcgagc gggacaggac cggttcgaag
      121 cgcttcgtga catccagcgc gaatgctacg acgacgcgac tgcaagaggt gtctccacgg
      181 cgaaggctcg attgagatag gccgcgcccg caaccaacga gatcctcggt gagagctcca
      241 atatggatcg cctggcgtga ttcgcggacg gaaaatagcg catccaccct ccctctggat
      301 tgatcgccat ggaagtactt ggcatcgaca tcggcggtac cggcatcaag gtggccaggg
      361 tcgacgtgcg caccggaaaa ctcacgacgg agttgatccg ctatcccacc cccgatccct
      421 cgacgcccaa gaaagtgctc ctggtcgtgc ggaaggcgat ccgggagttc gcctatgagg
      481 gcgcggtcgg ctgcggcttt ccgggggtcg tacgaaacgg cgtgattcaa accgcggcca
      541 acgtgtcgaa gaagtggatc gacgttcgcg ccgacgagct ctaccgggag gccaccggcc
      601 tgccgttctt cctcgcgaac gacgccgacc tcgcgggagt ggcggagatg cgcttcgggg
      661 cgggccgggg gcgcaagggg ctggtcatca tgctcacctt cggcacgggg atcggcaccg
      721 gggtcttcat ggacggcaag ctcgtcccca acagcgagct ggggcacctg gaggtccggg
      781 gccacgaggc ggagaaatgg gcgtcggagc ggacccgcat cgtgaagaag ctgagcttcc
      841 gggactgggc gtggcgggtg aacgtctacc tgaaccggct gcacgcctac ttctggcccg
      901 agctcttcat catcgggggc ggagtgagcc gggagttcga gaagttccgg aagtacctcg
      961 aggtgccctg cgagcttctg ccggcgaccc tccggaaccg ggcgggggtc atcggtgccg
     1021 cctatgccgc ctcccggaag ctgcgaatct gaaagaacgt tcggggcggc ttctgcgtcc
     1081 gatggaccgt cggtactcgg ctcggtctgc gcggaagtca cgaagtattg cgcaaggacc
     1141 tccgggcccg gaacgggatg gaaggtcgtg gcgagctggc cgggtgctgc tgtctcagtt
     1201 ccgagtagtc gagtggctgc gagaagggac ttcgaagcgc ttccaaccga agaggtgagc
     1261 atcgatagac catacgcccg gccccagcat cgctagacct gcagccattg agcccagcag
     1321 cagggaaacc catcggtctc cggccgacgt cagaatcgcc ccgatctgaa tcagggccac
     1381 cagggttccc acgacaggcg tccataagcc cacgatgagg agaagccccg ctatcgccaa
     1441 gaccccgcac atcatcaccg tggtcaccgg agggctactc gcaagcgtcg tccatccgcg
     1501 tgcgcccagg gcgaccccca atgcgaggcg catcacgaga agaccgact
//
LOCUS       DATITW010000294         1906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_14595, whole genome shotgun sequence.
ACCESSION   DATITW010000294 DATITW010000000
VERSION     DATITW010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14595"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="VLS86_04175"
     CDS             complement(<1..1221)
                     /locus_tag="VLS86_04175"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HSO49719.1"
                     /translation="MSNGDPGGESAPNGPVLELDGVYRQFGAVRALTDVSFDCRAGEV
                     HALVGENGSGKSTLLGIASGFVEPDRGSVRIGGKTLRTDSPALARKLGLAMAYQDTSS
                     ILAQPVKNNLYMAAPPEDRPAYWKRKKWARRLLGEFDLDFELFPDAPVGFLTLAERQL
                     FEVAKALVSNPKVLLLDEPTTALGPEEVEALHRTVAACRKRGVGVVYVSHRLPEILEI
                     ADRITVLRDGKTRGTFDARATSEHDLVELIVGRPFEAAFPPPSPVAGERREVLIVDSL
                     QGQSFGPVSFTLESGEVVGIAGAEGNGQPQLFDTLSGRTPPRAGRVTCEGKDLTLIST
                     HEAIRSGIMLLPGDRKHEALMPVLGVRVNVTVQSLRAFSRFGFLAWRAERRAVQELVD
                     QLEVRTPSLEQPVEF"
     gene            complement(1218..>1906)
                     /locus_tag="VLS86_04180"
     CDS             complement(1218..>1906)
                     /locus_tag="VLS86_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49720.1"
                     /translation="YVGLPGTDGALEPGVDYLSIVGENLCALGESFAQVINDGVGEGT
                     IGVLGGTPGNALSLGWQQCHIPALAEGIEVINPPADPNSTTGDTYWANDIARDVVSGF
                     LSTNPDISGFSYEYADGLYTALQDVEIDTMTIAIRTDEQTLFCDWVERDNPNYHIWYS
                     AGGNFQSRVAVTAQMMSLAGAEIPPVITVPHVMREVTASDCNPDRVTPTVSNTSLVPD
                     EVLTLMYPAG"
BASE COUNT          332 a          631 c          605 g          338 t
ORIGIN      
        1 gaactcgacg ggctgctcca atgacggcgt gcgaacctcc aactggtcga ccagttcctg
       61 caccgcacgg cgctccgccc gccacgccag gaacccgaac ctgctgaaag cgcggagtga
      121 ttgaacagtg acgttgacgc ggaccccgag gaccggcatc aaggcctcgt gtttgcggtc
      181 accgggcaag agcatgatcc cggatctaat ggcctcatgg gttgagatga gggttaggtc
      241 cttgccctca caggtgactc gacccgcccg gggaggcgtc ctccccgaca aagtgtcaaa
      301 gagctggggc tggccattgc cctcggcgcc ggcgattccg accacctccc cgctctccag
      361 ggtgaagctc accgggccga aggactgacc ctgcaagctg tcgacgatca gcacttcccg
      421 acgctcgccc gccaccggcg acggtggagg aaaggcagcc tcgaacgggc ggccgactat
      481 gagctccacc agatcgtgct ccgaagtcgc cctggcgtcg aatgtgccac gggtctttcc
      541 gtcccggagc accgtgatcc ggtcggcgat ctcgaggatc tcggggagac ggtggctcac
      601 gtacaccacg cccaccccgc gttttctgca tgcggccacg gtgcggtgga gtgcttccac
      661 ctcctcgggg ccgagagcgg tggtgggctc atcaagaagc agcaccttgg ggttggagac
      721 cagggccttg gccacttcga acagctgtcg ctcggcgagg gtgaggaacc cgaccggggc
      781 gtcggggaac agctcgaagt cgaggtcgaa ttcgcccagg agccgacggg cccacttctt
      841 cctcttccag taggccggtc tgtcctcggg cggcgccgcc atgtacaagt tgttcttgac
      901 cggctgggca aggatcgaag aagtgtcctg ataggccatg gccagaccca gcttccgggc
      961 caaggccgga gaatcggtgc gcaaggtctt cccgcctatg cgcaccgatc ctctgtcggg
     1021 ctctacgaac ccacttgcga tcccgaggag tgtcgacttc cccgagccgt tctctccgac
     1081 gagggcatga acctcgccgg cgcggcagtc gaagctgaca tcggtcagcg ctcgaactgc
     1141 gccgaactgg cggtagacac catccagctc gaggaccggc ccattgggcg ccgactctcc
     1201 tccgggatcg ccgttactca tccggccggg tacatcagcg tcaagacctc gtcgggaacg
     1261 agcgaggtgt tggacaccgt cggggtgacc cgatcggggt tgcagtcgga cgccgtcact
     1321 tccctcatca cgtgggggac ggtgatcacc ggcgggatct cggctccggc cagactcatc
     1381 atctgagccg tgaccgcgac cctcgactgg aagttgccac cggccgagta ccagatgtgg
     1441 tagttcgggt tgtcgcgctc cacccagtca cagaacaacg tctgctcgtc ggttcggatc
     1501 gcgatcgtca tcgtgtcgat ctcgacatcc tgcagcgcgg tgtacagccc gtcggcgtac
     1561 tcgtagctga acccggagat gtccggattc gtcgacagga acccgctgac gacatcgcgt
     1621 gcgatgtcgt tggcccagta ggtgtcccct gtggtcgagt tcggatccgc cggcgggttg
     1681 atcacttcga tgccttctgc cagtgccggg atgtggcact gttgccaccc gaggctcagg
     1741 gcattgcccg gtgtcccacc caggaccccg atcgtgcctt cgcccacacc gtcgttgatc
     1801 acctgggcaa agctctcgcc gagagcacaa aggttctcgc cgacaatgct caggtagtcg
     1861 acgccgggct cgagagcacc gtccgtcccg ggcaggccga cgtagc
//
LOCUS       DATITW010000295         1614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_535476, whole genome shotgun sequence.
ACCESSION   DATITW010000295 DATITW010000000
VERSION     DATITW010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535476"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VLS86_04185"
     CDS             complement(<1..747)
                     /locus_tag="VLS86_04185"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSO49721.1"
                     /translation="MTDPFRPDAEKGPSEMNDNKRHLLILGGGPAGTQAATTAASKGA
                     RVTLVESQIIGGAAHLWDCIPSKTMAASALRHSSVRNAAQLGLATEPTKVDTVVLARR
                     IEAITGDITRNWVDLLATQDVEVIRGRGRFVSAREVIVDTGSGERRIEFDRAMISTGS
                     APKVPDWAPVDGERVLTTRHCYDMADVPEHMIIIGSGVTGVEFTHIFESFGSMVSLVV
                     SRRQILPHRDAEVAAVLEEDFLERGVRLVIG"
     gene            complement(744..>1614)
                     /locus_tag="VLS86_04190"
     CDS             complement(744..>1614)
                     /locus_tag="VLS86_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSO49722.1"
                     /translation="RAAVEAGLDVDLFAPRLSFFWNGHNQFFEEVAKYRAARRIWARL
                     MRHQVGAKDPRSWAMRFHTQTAGSTLTAQQPLTNVVRTTVQALAAVLGGTQSLHTNSY
                     DEALGLPTDDSALLALRTQQVIAYESGVADTVDPLAGSFFVEYLTDQLESVANGLLGE
                     IEQLGGAVAAIEEGWMQARIEESAYREARRQGDGTSVVVGVNKFVSADGETIPVLEID
                     PALEEEQKRRLAGWKTGRDQAQVEDALDLLQRQAETDENLLPVMKTALRAGATLGEVS
                     DRLRSVFGAHRPG"
BASE COUNT          263 a          556 c          510 g          285 t
ORIGIN      
        1 cccgatcacc agtcgcacac cacgctcgag aaagtcctct tcgaggacgg ccgccacctc
       61 cgcgtcgcgg tggggcagga tctggcgacg ggagacgacg aggctgacca tcgatccgaa
      121 gctctcgaag atgtgggtga actcgacgcc ggtgaccccg gacccgatga tgatcatgtg
      181 ctccggcacg tcggccatgt cgtagcaatg gcgggtggtc aacacccgtt ccccgtcgac
      241 cggtgcccag tcggggacct tgggtgccga cccggtcgag atcatcgccc ggtcgaactc
      301 gattcgtcgc tcacccgagc cggtgtccac gatgacctcg cgggcagaca cgaaccggcc
      361 ccgaccacga atcacctcca catcctgggt ggcgaggaga tccacccaat tgcgggtgat
      421 gtcaccggtt atcgcctcga tcctcctggc gaggaccacg gtgtcgacct tggtcggctc
      481 ggtggcgagc ccgagttggg cggcattccg gaccgagctg tggcgcaggg cactcgccgc
      541 catggtcttg gatgggatgc agtcccagag gtgggccgcc cccccgatga tctggctctc
      601 gaccagggtg acgcgagccc ccttcgacgc cgcggtggta gccgcctggg tgccggcagg
      661 tcccccgccg aggatgagaa gatgtctctt gttgtcgttc atctcgctgg ggcctttctc
      721 cgcatcgggt cggaacggat cggtcaaccg ggacggtggg ccccgaacac cgaccggagc
      781 cgatcggaga cctcgcccag ggtcgccccc gccctcaatg ccgtcttcat caccgggagc
      841 aggttctcgt cggtctctgc ttggcgctgg agaaggtcga gagcgtcctc gacctgtgcc
      901 tgatcccgcc cggtcttcca cccggcaagc cgccgcttct gctcctcctc gagagccggg
      961 tcgatctcca gcaccgggat ggtctcaccg tcagcggaga cgaacttgtt gactccgacc
     1021 acgacactgg tcccgtctcc ttgtcttctc gcctcccggt atgccgactc ttcgatgcgg
     1081 gcctgcatcc atccttcctc gatcgcggcc accgccccac ccagctgctc gatctccccc
     1141 aacaggccat tggcgactga ttcgagttga tctgtcaggt actcgacgaa gaaggaaccg
     1201 gccagcggat ccaccgtgtc agccaccccc gactcgtagg cgatcacctg ctgggtgcgc
     1261 agggccagca gcgccgaatc gtcggtgggg agacccaggg cctcgtcgta ggaattggtg
     1321 tgcagggact gagtgccccc tagcaccgcg gccagggcct ggaccgtggt cctgaccacg
     1381 ttggtcaacg gctgttgcgc ggtcagggtc gagcccgcgg tctgggtgtg gaagcgcatg
     1441 gcccagctcc tgggatcctt ggcaccgacc tggtggcgca tcagtcgcgc ccagatcctc
     1501 ctcgcagccc gatacttggc cacttcctcg aagaactgat tgtgaccgtt ccagaagaag
     1561 gagagacgcg gggcaaagag atcgacatcg agaccggcct cgaccgccgc ccgc
//
LOCUS       DATITW010000296         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_334967, whole genome shotgun sequence.
ACCESSION   DATITW010000296 DATITW010000000
VERSION     DATITW010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334967"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VLS86_04195"
     CDS             <1..305
                     /locus_tag="VLS86_04195"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019744668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HSO49723.1"
                     /translation="HDDIDLAFGRLKLQVGGGSGGNNGIRSIESSLGTKGFSRLKVGV
                     GRPPGRIDPADFVLRPFTKTEREEVELIIEDAADIVEMWPSDPARAQEMAARRGRS"
     gene            312..1802
                     /gene="glpK"
                     /locus_tag="VLS86_04200"
     CDS             312..1802
                     /gene="glpK"
                     /locus_tag="VLS86_04200"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HSO49724.1"
                     /translation="MPDFVGALDQGTTSTRFMVFDHDGSVVASDQREHRQIFPKPGWV
                     EHDAVEIWSRATSVVEGAMSRAGLTTGDLAAVGLTNQRETTVVWDRATGQPVSNAIVW
                     QDTRTAELCARLGGDRGADRFRAKTGLPLATYFSGPKVAWLLDEMEIRDQAERGDLAF
                     GTIDSWLAWNLTGGTTGGHHVTDVTNASRTLLMDLATTTWDADLALEIGVPAAMLPAI
                     VPSIGVIDDCVGPLQGVPLAAILGDQQAALFGQACFDPGEAKNTYGTGNFMLLNTGHT
                     PVASEAGLITTVAFKRGEEPPLYALEGSVAVTGSLIQWLRDNLGLVSDASEVEALART
                     VEDNGDVYFVPAFSGLFAPHWRPDARGVIAGLTRFANSGHIARAALEATAYQTKDVID
                     AMVSDSGVELGELRVDGGMTANQLLMQFQADLLGVDVIRPRVSETTALGAAYGAGLAV
                     GFWSGQDEIRDLWAEDLRWQPTMGAEERERLHRRWQQAVERSFDWV"
     gene            1859..2734
                     /locus_tag="VLS86_04205"
     CDS             1859..2734
                     /locus_tag="VLS86_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO49725.1"
                     /translation="MFEELSLETFDLGEIRVRARVGGSGPPLLLLHGNPQTHVMWHKV
                     APALADRFSLVLPDLRGYGMSSKPPSTSDNAPYTKRTMALDQLALMRGLGHERFFVAG
                     HDRGGRVAYRLALDHPSAVLKLAVLDIIPTSEAFERGGREFGLALYHWYFLAQPSPLP
                     EKLIGSDPDWFWRWHTTRGPDTSFFDPVAVDDYLDCFRDPETIRGICEDYRAGATIDF
                     EHDIADKRAGRKIQCPLLVLWGSRSKPGSGYDPIAVWGDWADDVEGKALPCGHYLAEE
                     APEQTAEELSAFFSQ"
     gene            2817..3356
                     /gene="pgsA"
                     /locus_tag="VLS86_04210"
     CDS             2817..3356
                     /gene="pgsA"
                     /locus_tag="VLS86_04210"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HSO49726.1"
                     /translation="MALSRILLVPFIMAFTRSDRIDNALFIAAVLFAVAAFTDFLDGY
                     VARKTSGGTLLGAFLDTTADKILITGTLLALVSIDHVSIWPASIIIFREFAVMALRGM
                     VAAQGGLIAPSTWGKIKFGTQFLAIFLAFVRLPDRWGPWYLDQWVMLIAVIATVGSAW
                     GYLAAFWHTVRSEPTTTRS"
     gene            3353..>3730
                     /locus_tag="VLS86_04215"
     CDS             3353..>3730
                     /locus_tag="VLS86_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSO49727.1"
                     /translation="MKTGVTGGSGVVGRSVVRHLVAAGHEVSALARSDAAASGLEAEG
                     AGTVRGNVLDPKTLTRLVAGCVGVFHVAGINQLCPADPARMDLVNIEGTRNVIRAARD
                     AAVQRLVHTSSAVVIGERHGTIGT"
BASE COUNT          596 a         1211 c         1274 g          649 t
ORIGIN      
        1 tccacgacga catcgacctc gccttcgggc gtctcaagct ccaggtgggc gggggcagtg
       61 gcggcaacaa cgggatccgg tcgatcgagt ccagcctcgg caccaaggga ttcagcagac
      121 tgaaggtggg ggtggggcgg cccccgggcc ggatcgaccc cgccgacttc gtgctgcgac
      181 ctttcaccaa gaccgagcgt gaggaggtcg agctgatcat cgaggacgcg gccgacattg
      241 tcgagatgtg gccctccgac ccggcgcggg cccaggagat ggcagcccgg agagggaggt
      301 cctgacccgg catgcctgat ttcgtggggg ccctcgacca gggaaccacc tcgaccaggt
      361 tcatggtgtt cgaccacgat ggctcggtcg ttgccagcga ccagcgagag cacaggcaga
      421 tcttccccaa accgggctgg gtcgagcacg atgcggtcga gatctggtca cgggcgacct
      481 cggtggtcga aggcgccatg tcacgggcgg ggctcaccac cggtgacctg gcggcggtcg
      541 ggctgaccaa tcagcgcgag accaccgtcg tgtgggaccg cgccaccgga cagccggtta
      601 gcaacgccat cgtctggcag gacacccgca ccgcagagct ctgtgcccgt ctcggcggag
      661 accggggagc ggaccggttc cgggcgaaga caggtctccc tctcgccacc tatttctccg
      721 gccccaaggt ggcctggctg ctggacgaga tggagattcg agatcaggcg gagcgaggcg
      781 atctcgcgtt cgggaccatc gattcgtggc tcgcatggaa tctgaccggg ggcacgactg
      841 gtggtcatca cgtcacagat gtcaccaatg cctcgcggac gctgctcatg gatctggcca
      901 cgacgacctg ggatgccgat ctcgccctcg agatcggggt cccggcagcg atgcttcccg
      961 ccatcgtccc ttcgatcggg gtcatcgacg actgcgtcgg cccgctgcag ggcgtccctc
     1021 tcgccgccat cctcggcgac cagcaggcgg cgctgttcgg acaggcttgc ttcgacccgg
     1081 gagaggcgaa gaacacctac ggcaccggca acttcatgtt gctcaacacc ggtcacaccc
     1141 cggtggcctc cgaagcgggt ctcatcacca ctgtcgcctt caagcgtggg gaggagcctc
     1201 ccctgtatgc actggagggc tcggtggcgg tcaccgggtc gttgattcag tggttgaggg
     1261 acaacctggg gctggtgagc gacgcctcgg aagtcgaggc cctggcccgc acggtggagg
     1321 acaacggcga cgtctacttc gttcccgcct tctccggtct gtttgccccc cattggagac
     1381 cggacgcccg gggggtgatc gccggtctga ccaggttcgc caacagtggt cacatagcca
     1441 gggcggccct cgaggcgacg gcttatcaga ccaaggacgt catcgacgcc atggtgtcgg
     1501 attcgggtgt ggagctgggc gagttgcggg tggacggggg aatgacagcc aaccagctgc
     1561 tgatgcagtt ccaggcggac ctcctcgggg tcgacgtgat tcggccccgc gtcagcgaga
     1621 ccaccgcttt gggggccgcc tatggcgccg gtcttgcggt ggggttctgg tccggccagg
     1681 acgagatacg ggacctgtgg gccgaggacc tgcgatggca gccgaccatg ggagccgagg
     1741 agcgggagcg tctccatcga cgctggcaac aggcggtgga gcggtccttc gactgggtct
     1801 gagcgtctgg tgacggagaa ccgccgcgtc gggacctggc acgtggtgaa atgcgcgcat
     1861 gttcgaagag ctgagcctgg agacgttcga cctcggcgag atacgggtcc gggcccgggt
     1921 cggcggcagc gggccaccgc tcctcctcct ccacggcaac ccacagaccc acgtgatgtg
     1981 gcacaaggtc gcccccgccc tggccgaccg gttcagcttg gtgctgcccg accttcgtgg
     2041 ctacgggatg agctcgaagc cgccttcgac atcggacaac gctccttaca cgaagcggac
     2101 catggccctc gaccagctgg cgctgatgag agggctcggc cacgagcgtt tcttcgtggc
     2161 cggccacgat cgtggaggcc gtgtcgctta ccggttggca ctcgaccacc cgtccgcggt
     2221 gctcaagctc gcggtgctcg acatcatccc gaccagtgag gcattcgagc ggggtggccg
     2281 ggagttcggc ctcgccctct atcactggta cttcctggcc cagccctcac cactgccgga
     2341 gaagctgatc ggctccgacc ccgactggtt ctggcgctgg cacaccaccc gcggaccgga
     2401 cacgagtttc ttcgacccgg tggcagtgga cgactacctc gattgctttc gtgacccgga
     2461 gacgattcgc gggatctgtg aggactaccg cgccggggcc acgatcgatt tcgagcacga
     2521 catcgccgac aagcgcgccg ggcggaagat ccaatgcccg ctgctcgtgc tctggggctc
     2581 gaggtcgaag cccgggtccg ggtacgaccc gatcgcagtg tggggtgact gggcggacga
     2641 tgtggagggg aaggccctgc cctgtggcca ctatctcgcc gaggaggcgc cggagcagac
     2701 cgcggaggag ttgtcggcgt ttttctcgca atgaccgaag tgacctcctg attcccagcc
     2761 ctcaaccgat aagatcgatc caagtcgagg aggactgaat ctcaacccct gacatcctgg
     2821 ccctgtccag gattctcctg gtgccgttca tcatggcgtt cacccgatcg gatcggatcg
     2881 acaacgcgct cttcatcgcg gccgtcctgt tcgcggtcgc cgcattcacc gatttcctcg
     2941 acggctacgt ggcccgcaag accagcggag ggacacttct cggcgccttc ctcgacacca
     3001 ccgccgacaa gatcctgatc accggcaccc tgctcgccct ggtctcgatc gaccacgtgt
     3061 cgatctggcc ggcgtcgatc atcatcttcc gcgagttcgc ggtgatggcg ttgcggggaa
     3121 tggtcgccgc ccagggcggg ctgatcgccc cctccacctg ggggaagatc aagttcggca
     3181 ctcagttcct tgccatcttc ctcgccttcg tccggcttcc tgatcggtgg gggccctggt
     3241 atctcgacca gtgggtgatg ctgatcgcgg tgatcgccac cgtcggctcg gcttggggct
     3301 acctggcggc cttctggcac acggttcgct cggagccgac cacgacccga tcgtgaagac
     3361 tggcgtcacc ggcggatccg gtgtcgtcgg gcgctcggtg gtccgccatc tcgtcgctgc
     3421 gggccatgag gtctccgcac tggccaggtc ggacgccgcg gcgtcggggc tcgaggcaga
     3481 gggggcgggc acggtgcggg gtaacgtgct cgaccccaag accctcaccc gtctggttgc
     3541 cgggtgcgtc ggggtgtttc acgtcgcggg gatcaaccag ctttgccccg ccgacccggc
     3601 gcggatggac ctggtcaaca tcgaggggac gaggaatgtg atccgggcgg cgagggacgc
     3661 ggcggtgcaa cgtctggtcc acacctcgtc tgcggtggtg attggggaac ggcatgggac
     3721 catcggcacc
//
LOCUS       DATITW010000297         2043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_775770, whole genome shotgun sequence.
ACCESSION   DATITW010000297 DATITW010000000
VERSION     DATITW010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775770"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VLS86_04220"
     CDS             <1..835
                     /locus_tag="VLS86_04220"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HSO49728.1"
                     /translation="ISGGIHGDEINGVEVVREVMRALDARSLRGAVIAVPIVNPLGFL
                     MRSRYLPDRRDLNRAFPGSARGSTASRLAHLFMTEVVAHCSVGIDCHTASNHRMNIPQ
                     IRADVDDIETLRLARAFGAPFTIESRVRDGSLRQAATERGIKMMVYEAGQSDRFDEEA
                     IDFGVTGIMRTLRSMGMLDIALPEAEPTKVVRRTRWIRARRGGLVEIEPRLGDKVMTG
                     EVVARISDAFGTRPTAVKSTTTGWVIARTLSPLVNPGDPLLHVASESGPTLDELAEAA
                     P"
     gene            complement(832..1098)
                     /locus_tag="VLS86_04225"
     CDS             complement(832..1098)
                     /locus_tag="VLS86_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HSO49729.1"
                     /translation="MTFDVKFSPWREEAACVDVVDEVSFFPDPEDLAGIAAAKAVCAT
                     CPVAAECLSWSIETNQSEGICGGHTPKERRSIRRRWIEEIEKAS"
     gene            complement(1219..>2043)
                     /locus_tag="VLS86_04230"
     CDS             complement(1219..>2043)
                     /locus_tag="VLS86_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSO49730.1"
                     /translation="EPDVASSDATNIRTRIVKEGSEYRITGRKWFSSGAASPRCRISI
                     VMGVTDPDAEPHRRQSMVLVPMDAPGVTVEREPTVFGYQDRGGHPQILFDDVRVPVAN
                     LLGEQGGGFAIAQARLGPGRIHHCMRAIGAAERCLDLMVQRSQARTAFGRPLSDQGVI
                     GEWIADARIEIEQARLLVLKTAWMIDNVGVRAARTEIAAIKVVAPALLTRVADRAIQL
                     FGAAGFTDDYPLARFYSMGRWLQIADGPDEVHRRSVARAELGRLGSGAAPPPFVFD"
BASE COUNT          359 a          686 c          662 g          336 t
ORIGIN      
        1 gatctcgggt gggattcacg gcgatgagat caatggtgtg gaggtggttc gcgaagtgat
       61 gagggctctc gacgcccgga gtctccgggg cgctgtcatc gcagtcccga tcgtgaaccc
      121 gctcggtttt ctgatgcgat cgaggtacct gcccgaccgc cgggacctga accgggcctt
      181 tcccgggtct gcccgcggat cgaccgcctc gcgtctcgcc cacctcttca tgaccgaggt
      241 cgttgcccac tgctcggtcg ggatcgattg ccatacagcg agcaatcatc ggatgaacat
      301 ccctcagata cgggccgacg tcgacgacat cgagacgctg cgcctggccc gggcctttgg
      361 cgcacccttc accatcgagt cccgggttcg agacggttcc ctgcggcagg cagcgaccga
      421 gcgcgggatc aagatgatgg tgtacgaagc cggtcagtca gatcggttcg acgaggaggc
      481 gatcgacttc ggcgtcaccg ggatcatgcg gacgctgcgc tccatgggga tgctcgacat
      541 cgcgctcccc gaggccgagc cgaccaaggt ggtgcggcgg acgcggtgga ttcgcgcccg
      601 gagaggcggg ctggtcgaga tcgagccgag actcggggac aaggtgatga ccggcgaagt
      661 ggtggcgagg atctcagacg cattcggcac ccgacccacc gcggtcaagt ccacgacgac
      721 cgggtgggtg atcgctcgaa ccctgagccc cctggtcaac ccaggggacc cattgctcca
      781 cgtggcgagt gaatcagggc caactctgga cgagctcgcc gaggcagcgc cctaagacgc
      841 cttctcgatc tcctcgatcc agcgacgccg gatcgagcgt cgctcctttg gtgtgtgccc
      901 tccacagatt ccctcgctct ggttggtctc gatcgaccac gagaggcact cagcggccac
      961 agggcaggtg gcacacaccg ccttggcggc agcaatgcct gccaggtctt caggatcggg
     1021 gaagaaggac acctcgtcga ccacgtcgac acatgccgcc tcctcccgcc acggggagaa
     1081 cttcacatcg aaggtcatcg tgctaaccat tatcgagatt gggtaaacgc ctcatcaacc
     1141 actttcgttc ccgatccttc gcatggatca aacatccgag gtcggcgtga caagccgccg
     1201 ggagctcagc ctcgaacgtc agtcgaagac gaaaggtggc ggcgcggccc cggagccgag
     1261 acggccgagc tccgccctcg ccaccgagcg gcggtggact tcgtcggggc cgtcggcgat
     1321 ctgcagccac cgccccatcg agtagaagcg agccaacgga tagtcgtcgg tgaagccggc
     1381 cgccccgaaa agctggatgg cgcgatcggc gacccgggta agcagggcgg gtgccaccac
     1441 ctttatcgcg gcgatctcgg tcctggcggc cctcaccccg acgttgtcga tcatccacgc
     1501 cgtcttcagg accaacagcc gagcctgttc gatctcgatc cgggcatccg ctatccactc
     1561 gccgatcacc ccctggtccg agagcggccg gccgaacgcg gtgcgagcct gagaccgctg
     1621 caccatcaag tcgagacatc tctcggcggc accgatggcg cgcatgcagt gatggatccg
     1681 gcccgggccg agccgcgcct gggcgatggc aaagccaccg ccctgctccc cgagcaggtt
     1741 ggcgaccggc acccggacat cgtcgaagag gatctgggga tgccctcccc gatcctggta
     1801 cccgaagacg gttggctccc tctccaccgt caccccggga gcgtccatcg gcaccaggac
     1861 catcgattgg cggcggtggg gctcggcgtc gggatcggtg acacccatca cgatggagat
     1921 cctgcaacgg ggcgatgccg cccccgagct gaaccacttg cggccggtga tccggtactc
     1981 gctcccctcc ttcacgatcc gggtgcggat gttggtggcg tcggaactgg cgacgtcggg
     2041 ctc
//
LOCUS       DATITW010000298         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_375063, whole genome shotgun sequence.
ACCESSION   DATITW010000298 DATITW010000000
VERSION     DATITW010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375063"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..823
                     /locus_tag="VLS86_04235"
     CDS             137..823
                     /locus_tag="VLS86_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49731.1"
                     /translation="MTEAAIAVPSRPRTGDLTSFDPANFDWASFVLSPGKIVGLEGPS
                     GWGLTRLGLSLLAAPSQVGTVVVIDVRGWFSPLAAWQVGVNRHRLVVVRCAERRLWAQ
                     VTAAVLEGVAAVYAEIPAGVGENDLRRLAALTRARKAGLALRPLRGGLPPGITHMRVR
                     ASGVDWVGPDHGHGRLSGRQLSMEVSGKSIPLREVQFSDPSTGTSSSPPGVWCQVPGK
                     LAPSPRTIGV"
     gene            829..2364
                     /locus_tag="VLS86_04240"
     CDS             829..2364
                     /locus_tag="VLS86_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HSO49732.1"
                     /translation="MRTLCVWFPDWPLTDVEVEPDRPCFVFEDRPSRVSGGEAQRIRA
                     ANPAARQAGIEHGMGRRAAEALCPTALDLARDLAAETRRFEQVVLVLEGLIPRVEVVE
                     PGLAFVSVEGAILYYGGEGPLVERVDKELSNVIDQPRSGLRIGLAEGPFAARWAATSA
                     DQGPRVIEDTAAFLSTLDISTLEKDELIATFRWLGVSTLGELAGLPREAVASRFGYLG
                     LHAHRLASGEDQAPQPRSIPPHLAVEAFYDDPLETLDQVAFSARALSVRLMLGLRKEG
                     LAPHRVEITAAAANGKERSRTWRSADPFIAATLTERVWWQLRAWVEQGGVPGGLTRLR
                     LDPSDLSGDGRQLALLEPVAGLDGTLGWQGYDPGRPEVERALARAQALVGPEGVLQAT
                     PQGGRMPAEQVLWYRFGEEPGVAERDVSAPWPGATPGPSPALVPPEPVPFDIEWDEGI
                     PTRVRLGSRWEPVLNWAGPWRLTGRWWKGEPHADRYQLVTSAGAFLVVVRNGSSFLAG
                     VYD"
BASE COUNT          382 a          687 c          835 g          489 t
ORIGIN      
        1 gacagattcc agtgacagat tccagtgact ggggggcgtc gaatagcaca aggccaaact
       61 cggtagttcg cggtcttctt gctagtcgaa cgtatgttcg ataggctgcc tctccctgat
      121 ctttggtgga gtcgacatga cggaggcggc aatcgcggtt ccttcccggc cgcgaacggg
      181 cgatttgacc agtttcgacc cggctaattt cgattgggcc agcttcgtac tgtcgccggg
      241 gaagatcgtg gggctggagg gcccgtccgg gtgggggttg acccggctgg gcctttcatt
      301 gcttgccgct ccctcacagg tggggacggt ggtggtgatc gacgtgcgcg gctggttttc
      361 accattggcc gcctggcagg tgggggtgaa tcgccatcgc ctggtggtag ttcgctgtgc
      421 cgaacgcagg ttgtgggccc aggtgactgc tgcggtgttg gaaggagtgg cggcggtcta
      481 tgccgagatc ccagccgggg tgggcgagaa cgatttgcgc cggttggctg ccctcacccg
      541 ggcccgcaaa gccggattgg ccctgcgccc tctgcgtggt ggcctccctc ccgggatcac
      601 ccatatgcga gtccgggcaa gcggtgtgga ttgggtaggt cccgaccatg gacatggccg
      661 cctgagcggg cgacaactgt cgatggaagt ctccggtaag tcgatccccc tcagagaggt
      721 gcagttctct gatccatcaa ctgggacttc ttcttctcct cctggcgtct ggtgccaagt
      781 acccggaaaa ttggcgccaa gtccccgaac aattggcgtc tgatctcaat gagaaccctg
      841 tgcgtttggt ttcccgattg gccattgacc gatgtagagg tcgaaccgga caggccctgt
      901 ttcgtcttcg aggaccggcc atccagggtc agtggtggag aggcccagag aattcgggct
      961 gccaaccccg ctgctcgtca ggccgggata gagcacggga tgggcagaag ggcagccgag
     1021 gctctatgcc ctaccgctct tgatctggca cgtgatctgg ccgccgagac caggcgattc
     1081 gagcaagtgg tgctggtcct cgaagggttg atcccgcgtg ttgaagtagt cgagcccggt
     1141 ttggcatttg tgtcggtgga gggggcgatt ctctattacg gaggggaggg cccgctggtc
     1201 gagcgagtag acaaggagtt gtcgaatgtc atcgatcaac cgagaagcgg tttgcggatc
     1261 ggacttgccg aagggccttt cgcggcccgc tgggcggcga cctcggcgga tcaaggtccc
     1321 cgggtcatcg aggacacggc agcctttctc tcgactctcg atatctccac cctcgaaaag
     1381 gatgagctga tcgcgacatt ccgctggctc ggtgtgtcca ccctggggga gctggcaggc
     1441 ttgccccgag aggcggtggc atcacgcttc ggatacttgg gcctccatgc tcatcgcctg
     1501 gcgagcgggg aggaccaggc cccccagcct cgttcgatac ctccccatct ggcagtggaa
     1561 gccttctatg acgatcctct cgagaccctc gatcaggttg ccttctccgc ccgggctttg
     1621 tcggtccgtt tgatgctggg tctgcgcaag gagggtttgg ccccgcaccg ggtcgagatc
     1681 acagccgcgg ccgctaatgg caaggagcgc tcccgcacct ggcgttcggc cgatccgttc
     1741 atcgcggcga ctttgaccga aagggtctgg tggcagttgc gggcatgggt cgagcaaggg
     1801 ggagtgccgg gtggcctgac ccgactccgc ctcgatccct ccgatctctc cggtgatggt
     1861 cgtcagctgg ctctgctcga acccgtggcc gggctggatg gaacattggg atggcagggc
     1921 tatgacccgg ggcggccgga ggttgagagg gcactggctc gggcccaggc attggttggt
     1981 cccgaagggg tgctccaggc cacgccacaa gggggaagga tgcccgccga gcaggtgctg
     2041 tggtatcgat tcggagagga acccggcgtg gcggaaaggg atgtgtcggc tccctggcca
     2101 ggtgcgactc cggggccgag cccggcgctg gtcccccccg aaccggttcc attcgacatc
     2161 gaatgggatg agggaatccc aacccgggtc cgtctcgggt cgcgctggga acctgtgttg
     2221 aactgggccg gtccctggcg gctgaccgga cgctggtgga aaggagaacc gcatgccgat
     2281 cggtaccagt tggtcacctc agcgggtgct ttcctggtgg tggtcaggaa cgggagctca
     2341 ttcctggccg gggtttacga ctgagtccta ggcgtccttg ccgccgagtt cga
//
LOCUS       DATITW010000299         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_255002, whole genome shotgun sequence.
ACCESSION   DATITW010000299 DATITW010000000
VERSION     DATITW010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255002"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VLS86_04245"
     CDS             <1..708
                     /locus_tag="VLS86_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSO49733.1"
                     /translation="RSASVRRDFVANASHELKTPVSSIIASSEALQMAVERGDESAVR
                     FSDQIGASARQLGRLVADLLDLSRLERDEPELERLSLDHLVREELERIRPTAADIDVG
                     LEMDIAPVRVSGSRRDVAIAVRNLLDNAVRYTPPGGSISVELASLGDDAVLRIRDTGD
                     GIPTRDLNRVFERFYRVDNARARATGGTGLGLAIVKHVVESHGGSVDVESELGVGSTF
                     TVHLPVLAEDNAGAQLA"
     gene            719..>2073
                     /gene="dxs"
                     /locus_tag="VLS86_04250"
     CDS             719..>2073
                     /gene="dxs"
                     /locus_tag="VLS86_04250"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HSO49734.1"
                     /translation="MSILDSISSPADLRGLTQAELEQLSDEIRSFILDKVSKTGGHLS
                     PNLGVVELTLALHRAFDSPRDRFIWDVGHQAYVHKLVTGRMEGFDRLRHAGGLSGYPS
                     REESEHDFVENSHASTSLSYALGHALAGYPGYTVAIIGDGALTGGMAYEALNHIAVTK
                     PPRLIIVVNDNGRSYAPTVGGLAALATLAHYRFDPRYEKTKRTLGRMLRGLPLVGETA
                     DELAGRLKDAVKQILEPSTVFDALDLKYTGQIDGHDIALLEETFNTAKTYDQPVVIHV
                     VTEKGNGYQPAIEDELEKLHGVGSFDLTTGKALKTELRLSDVAGSALAEAAATRPDLV
                     GISAAMVSTAGLMQMSQRFPERVYDTGIAEQHAVTLAAGMALAGKRPVVAIYSAFLQR
                     AFDQIVDDVALHDLPVTFLIDRAGITGPDGPSHHGVFDISYLRMIPNMVVGVPADATE
                     L"
BASE COUNT          356 a          702 c          666 g          349 t
ORIGIN      
        1 cgctcggcgt cggtgcggcg tgacttcgtg gccaacgcat cgcacgagtt gaagaccccg
       61 gtgtcctcga tcatcgcctc gtcggaggcc ctgcagatgg cggtcgagcg cggtgacgag
      121 tcggcggtgc gcttttcgga ccagatcgga gcctccgccc ggcaactggg ccgtctggtc
      181 gccgacctgc tcgacctgtc ccgactcgag cgggacgaac cagagctgga acgtctgagc
      241 ctggaccacc tggtacgcga ggagttggag cgaatacgac cgacggcagc cgacatagac
      301 gtcgggctgg agatggacat tgccccggtt cgggtgtcgg gaagcagacg ggacgtggcg
      361 atcgcggtca ggaacctgtt ggacaacgcg gttcgctaca cacctcccgg gggctcgatc
      421 agtgtcgagt tggcctcgct cggcgacgat gcggtgctgc gaatcaggga caccggcgac
      481 gggatcccca cccgtgacct gaatcgagtg ttcgagcgct tctaccgggt ggacaacgcc
      541 cgcgcccgcg ccaccggtgg caccgggctc ggtctggcca tcgtcaagca cgtcgtggag
      601 agtcacgggg gcagcgtcga cgtcgagagc gagctggggg tggggtcgac cttcaccgtc
      661 catctcccgg ttctcgcaga ggacaacgca ggcgcccagc tagcctgagc acgcccccat
      721 gtccatcctt gattccattt cgagccctgc cgaccttcgt ggcctgaccc aggccgagct
      781 ggagcaattg agtgacgaga tccgctcctt catcctcgac aaggtctcca agaccggggg
      841 ccatctctcg ccaaacctcg gggtcgtgga gctcacactc gctctgcacc gcgccttcga
      901 ttcgccccga gaccggttca tctgggacgt cgggcatcag gcctacgtcc acaagctggt
      961 caccggtcgc atggagggct tcgatcgtct ccgccacgcg ggaggtcttt ccggataccc
     1021 gagccgggag gagtccgagc acgacttcgt tgagaacagc cacgcttcca catcgctctc
     1081 ctacgccctc ggtcatgccc tcgccggcta tcccggctac accgtggcca tcatcgggga
     1141 cggcgccctg accgggggca tggcctacga ggccctcaat cacatcgcgg tcaccaagcc
     1201 gccccgcctg atcatcgtgg tcaacgacaa tggccgctcg tatgcgccga cagtcggagg
     1261 cctcgccgct ctcgccacgc tcgcccacta ccgcttcgac cccaggtatg agaagacgaa
     1321 gcgcactctg ggccggatgc tcagaggtct tcctctggtc ggggagaccg ccgatgagtt
     1381 ggccggccga ctcaaggacg cggtgaagca gatcctcgag ccgtcgacag tcttcgacgc
     1441 actcgatctc aagtacaccg gtcagatcga cggtcatgac atcgccctcc tcgaggagac
     1501 cttcaacacc gccaagacct acgaccagcc ggtcgtgatc catgtcgtca ccgagaaggg
     1561 aaacgggtac cagccggcga tcgaggacga gctggagaag ttgcacgggg tcggctcctt
     1621 cgatctcacc acggggaagg cgctgaagac cgagctgcgt ctcagcgacg tcgccggcag
     1681 cgccctggcc gaggcggcgg ccacccgtcc cgacctggtc ggcatctcgg cggcgatggt
     1741 gagcaccgcc ggactgatgc agatgtcgca gcgattcccg gagcgggtct acgacacggg
     1801 gatcgccgag cagcacgcgg tgaccctcgc cgccgggatg gcgctcgccg gcaagcgacc
     1861 ggtggtcgcc atctactcgg cctttctcca gcgggccttc gaccagatcg tggacgacgt
     1921 cgccctccat gacctgccgg tgactttctt gatcgatcgg gccgggatca ccggtcccga
     1981 cgggccgtca caccatggcg tgttcgacat ttcatacctt cggatgatcc cgaacatggt
     2041 ggtgggggtc ccggccgatg ccaccgagct ctg
//
LOCUS       DATITW010000300         3810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_95165, whole genome shotgun sequence.
ACCESSION   DATITW010000300 DATITW010000000
VERSION     DATITW010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95165"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /gene="hutU"
                     /locus_tag="VLS86_04255"
     CDS             complement(<1..1246)
                     /gene="hutU"
                     /locus_tag="VLS86_04255"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HSO49735.1"
                     /translation="MIDLTFPLHAPHGSSLHTPGWLQEAALRCLLNNLDPEVAEDPVN
                     LIVYGGRGKAARNLECLHAIVRTLERLKDDETLLVQSGKPVGVFRTHEMAPRVLIANS
                     LLVPRWATLEHFWKLEAEGLMMYGQMTAGSWIYIGTQGILQGTFQTFAAIADQKFGGT
                     LAGTLTLTAGLGGMGGAQPLAVTMNGGAALCVEVDPHRIERRLETGYLDESYEDVDQA
                     VKRALQAKTDREAVSVGLLGNAAEVFPRLLEMGLDVDIVTDQTSAHDPLYGYVPAGYT
                     LEDAKRLREEDPDTYIKAARESMAAHCEAMVGFHQAGVETFDYGNNLRGEAQAAGYVD
                     AFSYPGFVPAYIRPLFCEGSGPFRWVALSGEPDDIAATDRALLDLFPDNEPLQRWLRL
                     ARDKVQFQGLPARICWLSYGERH"
     gene            complement(1341..2150)
                     /locus_tag="VLS86_04260"
     CDS             complement(1341..2150)
                     /locus_tag="VLS86_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49736.1"
                     /translation="MSVSAPTRLTPTGRMTPLRAQRIWERNLLVYRRLWKIIFTGFFE
                     PVFYLFSIGIGIGALVGEVTGPGGIPIPYTAFVAPALMAASAMNGAVLETTFNIFFKL
                     KFGNIYEGILTTPMNPRDIAIGEIGWALFRGALYSIAFLVVMVVMGLTDSWWAVMTIP
                     ASILIGFAFGSMGLAACTYMLSWQDFDLVSLVTMPLFLFSATFYPLDVYPPAIQQIAR
                     ISPLYHGTELLRAFSLGIFDWTLVGHAAFLLVMGLIGARIASRRLDGLLRK"
     gene            complement(2147..2935)
                     /locus_tag="VLS86_04265"
     CDS             complement(2147..2935)
                     /locus_tag="VLS86_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49737.1"
                     /translation="MSALTRPIRVVESELLAYRRTWRGTVISSFVNPVLFLSAMGVTL
                     GTLVDDSANDLAVPYLAFVATGLMAATAMQGGAGDGSFPVMAGIKWRKEFHGTITTPL
                     DPTDIVIGRFYWGAIRLTFILSVFAVIAMLFGALEAGPALLAVPPGILTGLAFQTTVT
                     AFTVTREDDISLSTLFRFGIIPLFLFSGTFFPISELPECLQRVAYATPLFHGVELVRK
                     IALPDVDSSVVTSLPIWVHIAYLLVMTAIGLVLASRFLERRLKP"
     gene            complement(2935..>3810)
                     /locus_tag="VLS86_04270"
     CDS             complement(2935..>3810)
                     /locus_tag="VLS86_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO49738.1"
                     /translation="FVAVDAVDFDIAKGEAFGFLGPNGAGKTSTMKMITTVSPITDGK
                     RSVFGLDPTTDGRAIRQRIGVVPQEDNLDLELTVAENLYVYGRYHDMDRSAIRARIVE
                     LLEFAQLSDRAKSKVEPLSGGMKRRLTIARALINDPDLVILDEPTTGLDPQARHVLWD
                     RLYRLKQQGATLIITTHYMDEAEQLCDRLVVMDGGKIVAEGSPRQLIAQYAPREVVEL
                     RMADGQVDVVASKLDGLANRTEILADRIILYTDDAEATQHELNVREIHPEQVLVRRST
                     LEDVFLKLTGRRLID"
BASE COUNT          709 a         1173 c         1304 g          624 t
ORIGIN      
        1 gatggcgctc tccgtatgag agccagcaga tccgggccgg cagtccctgg aactgcacct
       61 tgtctcgggc caaccgcaac catcgctgca gcggctcgtt gtcggggaag aggtcgagga
      121 gggcccggtc ggtggccgcg atgtcatcgg gctcaccgct caacgccacc cagcggaatg
      181 ggcctgaccc ctcgcagaac aggggcctga tgtatgccgg gacgaagccg ggataggaga
      241 aggcgtcgac atatccggcg gcctgcgcct cgccgcggag gttgttcccg tagtcgaacg
      301 tctccacacc cgcttggtga aagccgacca tcgcctcgca atgggccgcc atggactccc
      361 gggccgcctt gatgtaggtg tcggggtcct cttctctgag gcgtttcgca tcctcgaggg
      421 tgtatccggc gggcacatag ccatagagcg ggtcgtgggc cgaggtctga tcggtgacga
      481 tgtccacgtc gagacccatc tccagcagcc gggggaagac ctcggcggcg ttgccgagaa
      541 gacccacaga taccgcctcc cggtccgtct ttgcctggag agcccgtttt acggcctggt
      601 cgacgtcctc atagctctcg tcgagatagc cggtctccag cctcctctcg atgcggtggg
      661 gatcgacctc tacgcacaac gcagcgccac cgttcatggt gacggccagg ggttgggccc
      721 cacccatgcc accgagcccc gcggtcaggg tgagcgtccc ggccagggtc ccgccaaact
      781 tctgatcggc gatggcagca aacgtctgaa acgtgccctg gaggatgccc tgggtcccga
      841 tgtagatcca ggagcctgcg gtcatctgcc cgtacatcat caggccctcg gcttcgagct
      901 tccagaagtg ctcgagagtt gcccaacgag ggacgaggag gctgttggcg atcagcaccc
      961 gcggcgccat ctcatgagtg cgaaacaccc cgacgggctt gcccgactgc accaggagcg
     1021 tctcgtcgtc cttcagacgt tccagggtgc ggacgatggc gtgcaggcac tccaggttgc
     1081 gggcagcctt gccccggccg ccgtagacga tcaggttcac cgggtcctcg gccacctcgg
     1141 ggtcgaggtt gttgagcagg cagcgaagtg cggcttcctg aagccagccc ggggtgtgaa
     1201 gcgaagaccc gtgtggggcg tgaagcggga aggtgaggtc gatcatcgag tcgccgagcc
     1261 taggccaccc ccgtcattgg gacgctcagg ggtcatccgg acctggtgct cccaatttcg
     1321 agagcggttg tgggcggggg ctactttcgg agcaggccgt cgagccgtct cgaggcgatc
     1381 ctggccccga tcagacccat gacaagcaga aacgcggcgt ggccgaccag tgtccagtcg
     1441 aagatcccca gggagaacgc ccgcagcaac tcggtcccgt gatacagcgg cgagatgcgg
     1501 gcgatctgct ggatggccgg cgggtacaca tccagggggt agaaggtggc ggagaagagg
     1561 aagaggggca tggtcaccag cgacaccagg tcgaagtcct gccacgaaag catgtaggtg
     1621 caagcggcca gacccatcga gccgaaggcg aacccgatga ggatggaggc gggaatggtc
     1681 atgaccgccc accagctgtc ggtgagcccc atgaccacca tgacgacgag gaaggcgatg
     1741 gaatagagag caccgcgaaa cagggcccat ccgatctcac cgatggcgat gtcgcgcggg
     1801 ttcatcgggg tggtcaggat tccctcgtag atgttcccga acttgagctt gaagaagatg
     1861 ttgaacgtgg tctcgagcac cgcgccgttc atggccgagg cggccatcaa cgccggagcc
     1921 acgaaggccg tgtacgggat ggggatccca cccggcccgg tcacctcccc gaccaaagcg
     1981 ccgatcccga tcccgatcga gaacaagtag aacaccggct cgaagaagcc ggtgaagatg
     2041 atcttccaca agcggcgata gacgaggaga ttgcgctccc agatgcgctg cgcccgtaac
     2101 ggggtcatcc gcccggtggg agtgagccgg gtgggggcgg aaacgctcat ggcttgagcc
     2161 tccgctcgag aaagcgcgaa gcgagcacca gtccgatagc ggtcatcacc aggagatagg
     2221 cgatatgcac ccagatcggg agcgaggtca ccaccgagga gtcgacgtcg gggagggcga
     2281 tcttgcgcac cagctccacc ccatggaaca ggggagtggc gtaggcgact ctctgcaggc
     2341 actccggcag ctcgctgatg gggaagaagg tccccgagaa gagaaagagg gggatgatcc
     2401 cgaaccggaa tagcgtagag aggctgatgt cgtcctccct ggtcacggtg aaggcggtga
     2461 cggtggtctg aaatgcgagc ccggtgagga tcccgggcgg cactgcgagc agggcgggcc
     2521 cggcctcgag ggcgccgaac agcatcgcga tcaccgcgaa cacgctcagg atgaacgtga
     2581 ggcggatcgc accccagtag aagcgaccga tcacgatgtc tgtcgggtcg aggggggtgg
     2641 tgatcgtccc gtggaactcc tttctccact tgatcccggc catcaccggg aacgacccgt
     2701 caccggcgcc gccctgcatc gcagtggccg ccatgagccc ggtggcgacg aaggcgaggt
     2761 agggcacggc caggtcgttg gcactgtcgt cgaccagggt gcccagggtg acgcccatcg
     2821 cggagaggaa caacaccgga ttgacgaaac tggagatgac cgtcccgcgc caggtgcggc
     2881 ggtaggcgag cagctcgctc tccacgaccc ggatcggtct ggtgagggcg gacatcagtc
     2941 gatcagcctg cgcccggtga gcttgagaaa gacgtcctcc agggtcgatc ggcgcaccag
     3001 gacctgctcg ggatggatct ccctgacgtt cagctcgtgc tgggtcgcct cggcatcgtc
     3061 ggtgtagagg atgattcggt cggccaggat ctcggtccgg ttggccaacc catccagctt
     3121 ggatgcgacc acgtcgacct gcccatccgc catgcgcaac tcgaccacct cgcgtggcgc
     3181 gtactgagcg atcaactgac gtggtgaccc ctcggccacg atcttgccgc cgtccatgac
     3241 caccagccgg tcacagagct gttcggcctc gtccatgtag tgggtggtga tgatgagggt
     3301 cgccccctgc tgcttgaggc ggtagaggcg gtcccacagc acgtggcggg cctgggggtc
     3361 gagaccggtc gttggctcgt cgaggatcac caggtcgggg tcgttgatca aggcccgggc
     3421 gatggtcaga cgtcgcttca tccccccgga cagcggctcc accttggact tggcgcggtc
     3481 ggagagctgg gcgaactcga gcagctcgac tatccgggcc ctgatcgcgg agcgatccat
     3541 gtcgtgatac cgcccgtaga cgtagaggtt ctccgcgacc gtcagctcga ggtcgaggtt
     3601 gtcctcctgg gggaccacac ctattcgctg cctgatagcc cgaccgtcgg tggtcggatc
     3661 gaggccgaac acgctgcgtt tgccgtcggt gatcgggctg acggtggtga tcatcttcat
     3721 cgtcgaggtc ttgcccgccc cgttggggcc gaggaagccg aatgcctcgc ccttggcgat
     3781 gtcgaagtcg accgcgtcga cggcgacgaa
//
LOCUS       DATITW010000301         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_615752, whole genome shotgun sequence.
ACCESSION   DATITW010000301 DATITW010000000
VERSION     DATITW010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615752"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VLS86_04275"
     CDS             complement(<1..1126)
                     /locus_tag="VLS86_04275"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617181.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HSO49739.1"
                     /translation="MTTGLRIPRRYTSEGADPYSTIEWARRDSRITNPDGSVVFEVLD
                     AEVPASWSQVATDIVVSKYFRTAGVPQTDADGKPVLDGDGNPVLGSETSSRQVFDRLA
                     TTWRWWGEKHGYFASSEDAQAYEDEMKYMLANQIASPNSPQWFNTGLAHAYGITGTPQ
                     GFWYVDPETGEMTESPDSYTHPAPHACFINGVSDDLVNPGGIMDLWVREARLFKMGSG
                     TGSNFSQIRAENETLSGGGKSSGVMGFLRIGDRAAGAIKSGGTTRRAAKMVILDLDHP
                     DVEEFIDWKMHEEEKARILINHGGYAADFNGEAYATVSGQNSNNSVRVPNEFIEAVRN
                     DDDWQLRERTSGKVRKTIKARDLWRKVAEAAWACADPGVQF"
BASE COUNT          278 a          434 c          492 g          305 t
ORIGIN      
        1 cgaactggac gccggggtcg gcgcaggccc aggcagcctc ggccaccttg cgccagagat
       61 cgcgagcctt gatggtcttg cggaccttgc cgctggtgcg ctcacgcagt tgccagtcgt
      121 cgtcgttgcg gacggcttcg atgaactcgt tgggaacccg caccgaattg ttggagttct
      181 gcccggagac ggtggcgtaa gcctcgccgt tgaagtcggc ggcataccca ccatggttga
      241 tgaggatgcg ggccttttct tcctcgtgca tcttccagtc gatgaactct tcgacgtcgg
      301 gatggtcgag gtcgaggatg accatcttgg cggcgcgacg ggtggtgcct ccactcttga
      361 tggcgccagc ggcgcggtcg ccgatgcgaa ggaagcccat cacgcccgat gacttgccgc
      421 caccggagag ggtctcgttc tcggctctga tctgagagaa gttggacccg gtgcccgaac
      481 ccatcttgaa gaggcgagct tcccggaccc agaggtccat gatcccgccg gggttgacga
      541 ggtcgtcgct gacaccattg atgaagcaag catgcggtgc cgggtgggtg taggagtcgg
      601 gggattcggt catctcgccg gtctcggggt cgacgtacca aaagccctgg ggagtgccag
      661 tgatgccata ggcatgagca aggccggtgt tgaaccattg cggagagttg ggagaggcga
      721 tctggttggc gagcatgtac ttcatctcgt cttcgtaggc ctgggcgtcc tccgaagacg
      781 cgaagtagcc gtgcttctcg ccccaccagc gccaggtggt ggcgaggcgg tcgaagacct
      841 ggcgactcga ggtctcggag ccgaggaccg ggttgccgtc gccatcgagg acgggcttgc
      901 cgtcggcgtc ggtctggggg acgccggctg tgcggaaata cttggagaca acgatgtcgg
      961 ttgcgacctg cgaccacgat gccggcacct cggcgtcgag aacctcgaag accaccgaac
     1021 cgtcggggtt ggtgatgcgg gaatcccgtc gggcccactc gatggtcgaa taggggtcgg
     1081 cgccttctga ggtgtaccga cgaggaatcc gtaggcctgt tgtcatctgt gttctcctct
     1141 ccagagaatc cggggtcccc tctgcttcta gtgagcggat aaggggccca aacgtcccga
     1201 gtcaacgtgc attcccacca attgacccac aatatgttgt ggttgcctac cccctgacca
     1261 caacctattg gggcgaatac acgactgtaa ttacaagctt gtcactggtc aaggaccttc
     1321 ttagatcggt tttcgcgacc tctcctagag cgcgaggaaa cgcctgaaaa tcaatgggga
     1381 agtgtccgcc ggggaagcgg ttgacgaaca gttggcaacc tagtcctccc ctcctccagc
     1441 gcgaaaattg tcttaaaggg aacttttcgc ggtcggagta gctgacacca tccggctgac
     1501 cgcaatacc
//
LOCUS       DATITW010000302         1503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_15127, whole genome shotgun sequence.
ACCESSION   DATITW010000302 DATITW010000000
VERSION     DATITW010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15127"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VLS86_04280"
     CDS             complement(<1..946)
                     /locus_tag="VLS86_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49740.1"
                     /translation="MATAVATASGAQRGLPRILRSPKTARILFPFVVLGIWYFIYYTL
                     DSRIFPSPQQVAVFMWEEITLTSGVRYSSVAENLYVQFGITLMRLTSGFFIALIVGTI
                     IGLAMGVSKKVDAFFHDWVMGLLAMPALVYALFLGLALGFTNVGPIIAVVLAGAPFVI
                     INIREGVRNTPRELIDMARSFEVPQNKVTRHVLLPSLMPFFFAAIRYAYAVGWKGLVI
                     TEVFASDRGMGWTIKFWYDAHRAHGVIGYALFFIIFALALEKLVFDPLADRAFKWRPK
                     MEAVQMAEGDFGGVQREGFLFIEDQEIEEGADTTVAKGG"
     gene            complement(946..>1503)
                     /locus_tag="VLS86_04285"
     CDS             complement(946..>1503)
                     /locus_tag="VLS86_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSO49741.1"
                     /translation="GLAFLVGIVIGILTQSRWWHGFFRDSVTISLVSPSLIWALTGLV
                     IWGFEPQGWIFAIVMTTFAVVTINVAEGIRALPRDLIDMSRAFGVTNWRRQREIVFPF
                     LAPFVFTALRFGFSVGWKVTVLTEVFSSNEGIGFEMRVAQQLFRMDEFFAWAISFFIF
                     ALFLEKVVLQRSERRFFRWRQEVAS"
BASE COUNT          340 a          451 c          466 g          246 t
ORIGIN      
        1 tcccaccctt tgcaacggtt gtatccgcgc cttcttctat ttcctggtcc tcgatgaaga
       61 ggaagccctc acgttgaact ccgccgaagt cgccctctgc catctggaca gcttccatct
      121 tgggtcgcca cttgaaggcc cgatcggcga gtgggtcgaa caccagtttc tccagagcaa
      181 gggcaaagat gatgaaaaag agggcgtagc cgatcacacc gtgggcgcga tgggcgtcgt
      241 accagaactt gatggtccac cccatgcccc ggtccgaggc gaacacctcg gtgatgacca
      301 gacccttcca cccgaccgcg taggcgtagc ggatggcggc gaagaaaaac ggcatcagcg
      361 aggggaggag gacgtgacgg gtcaccttgt tctgggggac ctcgaaggac cgcgccatgt
      421 cgatgagctc tctcggagtg ttgcgcaccc cctcccggat attgatgatc acgaaaggtg
      481 caccggccag gaccaccgcg ataatcgggc cgacgttggt gaatccgagg gccaagccga
      541 ggaagagggc atagaccagt gcgggcatcg ccagcaatcc catgacccag tcgtggaaga
      601 aggcgtcgac cttcttggat acccccatgg ccagaccgat gatcgtgccc acgatgagtg
      661 cgatgaagaa gccgctggtc agccgcatca aggtgatgcc gaactggaca tagaggtttt
      721 ccgccacgct cgagtagcgg actccgctgg tgagggtgat ctcctcccac atgaacaccg
      781 caacttgctg cggggacggg aagatcctcg agtcgagtgt gtaatagatg aagtaccaga
      841 tccccaggac gacgaagggg aagaggatcc gggcggtctt cggcgacctg aggatcctgg
      901 gcagtcctcg ctgtgcgccg gatgcggtcg cgacggccgt ggccatcagg acgccacctc
      961 ctgacgccaa cggaagaatc tccgctcaga gcgctggaga acgaccttct ccaggaacaa
     1021 tgcgaagatg aagaaggaga tcgcccacgc gaagaattcg tccatccgga acagctgttg
     1081 tgccacccgc atctcgaagc cgattccctc gttggaagag aacacctcgg tgagcaccgt
     1141 caccttccag cccaccgaga acccgaaccg gagggcggtg aatacgaacg gggcgagaaa
     1201 cggaaacacg atctctcgct gccgacgcca attggtgact ccgaacgctc gggacatgtc
     1261 gatcaggtcc ctgggcagtg cgcggatccc ctctgcgacg ttgatggtca ccacggcgaa
     1321 ggtggtcatc acgatggcga agatccatcc ctgtggctcg aacccccaga tcaccagccc
     1381 ggtcagcgcc cagatcaggg aaggagagac caggctgatg gtgaccgagt ccctgaagaa
     1441 gccgtgccac cagcggctct gggtgagaat gccgatgacg atcccgacga ggaaggcgag
     1501 gcc
//
LOCUS       DATITW010000303         1894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_576089, whole genome shotgun sequence.
ACCESSION   DATITW010000303 DATITW010000000
VERSION     DATITW010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576089"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..852
                     /locus_tag="VLS86_04290"
     CDS             34..852
                     /locus_tag="VLS86_04290"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HSO49742.1"
                     /translation="MAEFMSLRESIRSAVHDGQTVALEGFTHLIPFAAGHEIIRQGRV
                     DLTLIRMTPDLVYDQMIGMGCAGKLVFSWGGNPGVGSLHRFRDAVERSWPRPLVIEEH
                     SHAAMAAAWAAGAAGLPIALLRGYLGTSLPSVNPRIEFVTDPFSGEQLAAVPALEPDV
                     GIIHAQRADRDGNVLLEGIVGIQKEVVLASKVSIVTVEEVVDDLASPSLNSVVLPGWA
                     IDLIVAAPGGARPSYAHGYYRRDNAFYLEWDEISRDRERFTSWMQSNVLEAADG"
     gene            845..1729
                     /locus_tag="VLS86_04295"
     CDS             845..1729
                     /locus_tag="VLS86_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018096129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HSO49743.1"
                     /translation="MADYTSTEVMTVSASRALAGDDVCFVGIGAPSAACNLARLTHAP
                     GITLIYESGTIGVRPDVLPLSIGDGELADTAVTTVSVPEMFQYWLQGGRVSIGFLGAA
                     QLDRFGNINTTVIGSYEAPKVRLPGAGGAPEIAAHCREVFVVMEHNSRALVPEVDFVT
                     SLGFGPSGTEREKLGITTKGPTLVVTDLALLQPEPGSNELVVTSMHPGVTTEEIRAKT
                     GWPVRFSADLGQTPTPTDTELTLLRELQERTNRAHQGAAERERRAFPPSAAGGSLHCR
                     WGQGVDVGDHVEHHRPVG"
     gene            complement(1658..>1894)
                     /locus_tag="VLS86_04300"
     CDS             complement(1658..>1894)
                     /locus_tag="VLS86_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSO49744.1"
                     /translation="KRGFDRVYGGKQSEKAREMWVFTEVDFAALAEHIGAIGIRVETP
                     ADLASALDRALSADRPVVLDVVTDIDALAPPAVE"
BASE COUNT          294 a          641 c          631 g          328 t
ORIGIN      
        1 tgacggcgac gagaccgtat tctccgagct cttgtggccg agttcatgtc actccgggag
       61 agcatccgca gcgccgtcca tgatgggcag acggtggccc tcgagggctt cacccatctc
      121 atcccattcg ctgccggtca cgagatcatc cgccagggtc gggtcgacct gaccctgatc
      181 cggatgactc ccgacctcgt ctatgaccag atgatcggga tgggctgtgc cggcaagctc
      241 gtgttctcct gggggggcaa ccccggggtg ggatcgctgc accgtttccg ggacgcggtg
      301 gagagatcct ggcctcggcc cctggtgatc gaggagcaca gccatgccgc catggccgcc
      361 gcctgggcgg ccggcgccgc gggacttccc atcgcactcc tccggggtta cctgggcacc
      421 agtctcccct cggtcaatcc gcgcatcgag ttcgtgaccg accctttcag cggcgagcag
      481 ctggcggctg tccccgcgct cgagccggat gtcgggatca tccacgccca gcgggcggat
      541 cgcgacggga acgtgcttct ggaagggatc gtggggatcc aaaaggaggt ggtcctggcg
      601 tcgaaggtct cgattgtcac cgtcgaggaa gtcgtcgacg atctcgcctc cccgagtctc
      661 aacagtgtcg tcctcccggg gtgggccatc gacctgatcg tcgcggctcc cggtggtgcc
      721 cgaccctcct atgcccacgg ctactaccgg cgggacaacg ccttctacct ggaatgggac
      781 gagatctccc gcgaccggga gagattcacg tcgtggatgc agagcaacgt cctcgaggcg
      841 gcggatggct gattacacgt ccacggaggt gatgaccgtc agtgcctcgc gggcgctggc
      901 cggcgacgat gtctgcttcg tgggcatcgg ggcgccctcg gcggcctgca atctggctcg
      961 actcacccac gcccccggga tcacgctgat ctacgagtcg gggaccatcg gggttcgacc
     1021 cgacgtcctc ccgctgtcga tcggtgacgg cgagttggcg gacaccgcgg tgacgaccgt
     1081 gtccgtgcct gagatgttcc agtactggct tcagggaggc agggtctcga tcggcttcct
     1141 cggcgccgcc cagctcgacc ggttcggcaa catcaacacc accgtgatcg gttcctacga
     1201 ggcacccaag gtgagactcc ccggcgccgg cggggccccc gagatcgcgg ctcattgtcg
     1261 tgaggtcttc gtcgtcatgg agcacaacag ccgggcgctg gtccccgagg tcgacttcgt
     1321 cacctcgctc ggctttgggc cttcgggcac cgaacgggag aagctgggaa tcaccaccaa
     1381 ggggccgacg ctggtggtga ccgacctcgc cttgctccag cccgagccgg ggagcaatga
     1441 gctggtggtg acctcgatgc atcccggtgt caccaccgag gagatccggg ccaagaccgg
     1501 gtggccggtc cgattcagcg ccgacctggg gcagacccct acacccaccg ataccgagtt
     1561 gacgctgctt cgcgagcttc aggagcggac caaccgggct caccaggggg ctgcggagcg
     1621 tgagcgccgg gccttcccac cctcagcagc cggtgggtca ctccactgcc ggtggggcca
     1681 gggcgtcgat gtcggtgacc acgtcgagca ccaccggccg gtcggctgac agagcccggt
     1741 ccaaggcgga ggccaggtcg gcgggcgttt ccaccctgat cccgatggca ccgatgtgct
     1801 cggcgagagc ggcgaagtcg acttcggtga acacccacat ctcccgggcc ttctcgctct
     1861 gcttgccccc gtagacccgg tcgaagcctc gttt
//
LOCUS       DATITW010000304         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_655792, whole genome shotgun sequence.
ACCESSION   DATITW010000304 DATITW010000000
VERSION     DATITW010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655792"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VLS86_04305"
     CDS             <1..614
                     /locus_tag="VLS86_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HSO49745.1"
                     /translation="GEATDLGSGFQLALRDLEIRGAGSILGEVQSGHIAAVGFDLYAE
                     LVAEAVAELEGREPVVAPQHEVRIDLPVDAHLPDGYVADQELRLEAYRRLATTTTNQE
                     VDDVAAEWVDRFGPMPPAALALVALARLRVEALRVGLEEMVQLRHEIRLGPVDLKPSQ
                     EVRLKRIQPRSVLQAAEGLIFIPAPRDLVPGLIDFLREMWEPT"
     gene            706..1614
                     /locus_tag="VLS86_04310"
     CDS             706..1614
                     /locus_tag="VLS86_04310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSO49746.1"
                     /translation="MKKLASLLVLAALVLAACGGGSNATVATVNGEDVTLGEIEALID
                     PGEETTIAKDVFARFLGFDIQQRIVLSAAEGEFGIVIAEDDIAAEADAIFEEANVEGV
                     SREEFLASNGITEELLTKLAHQQLLDTQIREMFAAETEDPTQEEIDGVITESEALYCS
                     SHILVATEEEANDVLDRIEGGEEFADLAAELSTDTGSGAQGGDLGCTSPDQYVTEFAD
                     ALVAAEVGVPTEPVETEFGFHIILLGEDVVPSNEEIIEQLRTTSIGTATNEWFAEQVE
                     NAEVTVDDSYGTWEASPVPQVVPPTE"
     gene            1611..>2228
                     /locus_tag="VLS86_04315"
     CDS             1611..>2228
                     /locus_tag="VLS86_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HSO49747.1"
                     /translation="MITITGLGPGDFDRIPETVRSLLLDRTRKLIVRTINHPAAEQLA
                     GMRDVMACDDLYESADSFEGVYTAIAERVLAAGREGPVVYAVPGSPLVGEFAVRHLLS
                     SGSEIELIPAESFVDAILAEAGYDPLDRGLQILNGHDLPRPLVLDKPTIVGHLDRPEI
                     MAEVSAQIARVLPEEATVTVFAGVGSADSIRVESAPDDVNSSLAGF"
BASE COUNT          374 a          733 c          732 g          389 t
ORIGIN      
        1 tcggcgaggc caccgatctc ggctctgggt tccaactggc attgcgtgat ctggagatcc
       61 gtggcgccgg gtcgattctg ggcgaggtgc agagcggcca catcgcggcg gtcggcttcg
      121 acctgtacgc cgagctggtg gcggaggcgg ttgcggagct cgagggacgg gagccggtag
      181 tcgcccccca gcatgaggtg cggatcgatc ttcctgtcga cgcccatctc cccgatggct
      241 acgtcgccga tcaggaattg aggctcgagg cgtatcgccg gctcgccacc actaccacca
      301 atcaagaagt cgatgatgtc gccgccgaat gggtcgaccg cttcggcccg atgcctcccg
      361 ccgctttggc cttggtggcc ctggcccggc ttcgggtcga ggcactccgg gtgggtctcg
      421 aggaaatggt ccagctccgc cacgagatcc ggctgggccc ggtcgatctc aagccgtcac
      481 aggaggttcg tctcaagcgg atccagcccc gttcggtgct ccaggccgcc gaggggctga
      541 tcttcatccc ggctcctcgg gacctggtgc cggggctcat cgacttcctc agggagatgt
      601 gggagccgac ctgatcccgg gcacaacgaa tgtgctctct gcttattggt tgccgctctc
      661 gtgctctacc attccgctgt tcccctttcc cagtgaggat cccccttgaa gaagctcgca
      721 tcactcttgg tcctggccgc gctcgtgctc gccgcctgtg gcggcgggtc gaacgccacc
      781 gttgccaccg tcaatggcga ggacgtcact ctcggcgaga tcgaagcact catcgacccc
      841 ggtgaggaga cgacgatcgc caaggatgtc ttcgcccgtt tcctcggatt cgacatccag
      901 cagcggatcg tgctcagcgc cgccgaggga gagttcggga tcgtcatcgc cgaggacgac
      961 atcgcggccg aggccgatgc catcttcgag gaggcgaatg tcgaaggggt aagtcgcgag
     1021 gagttcctcg ccagcaacgg catcaccgag gagcttctga ccaagctggc acaccagcag
     1081 ctgctcgaca cccagatcag ggagatgttc gccgcggaga ccgaggatcc gacccaggag
     1141 gagatcgacg gcgtgatcac cgagtccgag gcgctctact gctcttcgca catcctggtg
     1201 gccacagagg aggaggccaa cgatgtcctc gatcggatcg agggcggaga ggagttcgcc
     1261 gacctcgctg ccgagctctc caccgacacc ggctccggtg ctcagggtgg ggacctgggg
     1321 tgcacctccc ccgatcagta cgtgaccgag ttcgccgacg ctctcgtcgc tgcggaggtg
     1381 ggagtcccca ccgagccggt ggagaccgag ttcgggttcc acatcatcct cctcggagag
     1441 gacgtcgtgc cgagcaacga ggagatcatc gagcagctgc ggaccacgtc gatcggcacc
     1501 gccaccaacg agtggttcgc cgagcaagtg gagaatgccg aagtcacggt cgacgacagc
     1561 tacggcacct gggaggcgag cccggtccca caggtcgtac ccccgaccga gtgatcacga
     1621 tcaccggtct cggccccggc gacttcgacc ggattccgga gacagtccgc tcccttctcc
     1681 tcgatcggac acgaaagctg atcgtgcgga cgatcaatca ccctgcggcc gagcaactgg
     1741 ccggcatgag agacgtcatg gcctgtgacg atctctacga gagcgcagac tccttcgagg
     1801 gggtgtacac cgcgatcgcc gagcgggttc ttgccgcggg gcgagaaggc ccggtggtct
     1861 acgcggtgcc cggaagcccg ttggtcggcg agttcgcggt caggcacctg ctctcttcgg
     1921 gctctgagat cgagctgatc cccgccgagt cgttcgtcga cgccattctc gccgaggccg
     1981 gctacgaccc cctcgaccga ggtctccaga tcctcaacgg gcacgacctg ccccggcccc
     2041 tggtcctgga caagccgacg atcgtcggcc atctggatcg acccgagatc atggccgagg
     2101 tgtcggcgca gatcgcccgg gtcctccccg aagaggcgac ggtgaccgtg ttcgccggag
     2161 tcgggtctgc agactcgatc cgggtggagt cggcgccgga cgacgtcaac tcgagtctgg
     2221 ccggcttt
//
LOCUS       DATITW010000305         1940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_655879, whole genome shotgun sequence.
ACCESSION   DATITW010000305 DATITW010000000
VERSION     DATITW010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655879"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1940
                     /locus_tag="VLS86_04320"
     CDS             <1..>1940
                     /locus_tag="VLS86_04320"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HSO49748.1"
                     /translation="IDRPLRPSFADDYRIETHVVVTSLAVDQVNPFDVVALNGASAAL
                     AVSPIPFEGPIGGIRLALKKGEWIPFPTQEDLDESVFELVVAGGRNDEGGIDIIMVEA
                     GSTPNGLRMIAAGDSPSDEAAVVAGLEASKAYIAQLIDLQDELVSKVTKVGFDWPTVV
                     DYSEEMHDRVLAIAEPKLASVVTIADKQERNRAQDEAQDAVLAELGLADDDEESRGQA
                     KRAFKSILKDMMRKRVVEDGVRLDGRAADEIREITVEVGVVGRTHGSALFQRGETQVL
                     NLTTLGMLRMEQMLDTLALEDSKRFMHHYNFPPFSTGEAGFMRGPKRREIGHGALAEK
                     AIHPVVPTEEEFPYALRLVSEVLSSNGSTSMASVCASSLSLMEAGVPISDAVAGIAMG
                     LIHRDGKYVTLTDILGAEDALGDMDFKVAGTKDVITALQLDTKLVGLPSEVLTTALDQ
                     ARVARLFILDKMNAVIAEPRAEMNEFAPRIESIEIPKDKIGEVIGPKGAVIRELEEQT
                     GAKLESEETDGKGIVRIASADGEALAAAKERVMQIAFPPEVELGTEYEGKVVNITKFG
                     AFVNILPGRDGLLHISRLDGSKRVERVEDYLTDGQVLKVRVREIDRGKVSLELVEALE
                     GATLPDPEAPSGGGGRREGGGR"
BASE COUNT          351 a          596 c          668 g          325 t
ORIGIN      
        1 atcgaccgac ccctccgtcc ctcgtttgcc gacgactatc gcatcgagac ccacgtcgtg
       61 gtcacctcgc tggctgtcga tcaggtgaac ccgttcgacg tcgtcgctct caacggggcc
      121 tcggctgccc ttgcggtgag cccgatcccg ttcgagggcc cgattggcgg gattcggctt
      181 gccttgaaga aaggcgagtg gatccccttc ccgacccagg aggatctcga cgagtcggtg
      241 ttcgagctgg tggtcgccgg cggccgcaac gacgaaggcg ggatcgacat catcatggtg
      301 gaggcaggct ccacccccaa cgggctgcgg atgatcgcag ccggtgactc gccctcggac
      361 gaggccgcgg tcgtggctgg tctcgaggcg tcgaaggcct acatcgccca gttgatagac
      421 ctccaggacg aactggtgtc caaggtcacc aaggtcggtt tcgattggcc gaccgtcgtc
      481 gactactccg aggagatgca cgacagggtg ttggccatcg cggagcccaa gctggcttcg
      541 gtggtcacca tcgccgacaa gcaggagcgc aaccgggctc aggacgaggc acaggatgcc
      601 gtcctcgccg agttgggcct ggcggacgac gacgaggaga gccggggcca ggccaagcgc
      661 gccttcaagt cgatcctgaa ggacatgatg cggaagcggg tggtcgagga cggagtgcgt
      721 ctcgacggcc gcgccgcgga cgagatccgc gagatcaccg tggaggtcgg cgttgtcggt
      781 cgcacccacg ggtcggcgct gttccaaagg ggcgagaccc aggtgttgaa cctgaccact
      841 ctcggcatgc ttcgcatgga gcagatgttg gacaccctgg ctctcgagga ttccaagcgg
      901 ttcatgcatc actacaactt ccctccgttc tcgacgggcg aagccggttt catgcgtggc
      961 cccaagcgac gcgagatcgg tcacggggca ctggccgaga aggcgattca cccggtggtc
     1021 cccaccgagg aggagttccc ctatgccctg cgcctcgtgt ccgaagtgct gtcctcgaac
     1081 gggtcgacat cgatggcatc tgtctgcgcc tcgtcgctct cactgatgga ggccggagtc
     1141 ccgatctccg atgcggtcgc cggaatcgcc atggggctga ttcaccgcga cggcaagtac
     1201 gtcacgctca ccgacatcct gggagcggag gacgcactcg gtgacatgga cttcaaggtg
     1261 gcgggaacca aggatgtgat cactgccttg cagctggaca ccaagttggt gggtctccct
     1321 tcggaggtgc tgaccaccgc actggaccag gcgcgggtgg cccggctctt catcctggac
     1381 aagatgaacg cggtgatcgc cgagcctcgg gccgagatga acgagtttgc gccacgcatc
     1441 gagtcgatcg agatccccaa ggacaagatc ggtgaggtca tcggccccaa gggcgctgtg
     1501 atccgggagc tcgaagagca gaccggggcc aagctcgaga gcgaggagac cgacggcaag
     1561 ggcatcgtcc gcatcgcatc cgcagacggt gaggcactgg ctgcggccaa ggagcgggtg
     1621 atgcagatcg ccttcccgcc cgaggtcgag ctgggcacgg agtacgaggg caaggtggtc
     1681 aacatcacca agttcggcgc cttcgtgaac atcctccccg gccgtgacgg gttgctccat
     1741 atctcccgtc tcgatgggtc gaagcgggtg gagcgggtcg aggactatct caccgatggc
     1801 caggtgctca aggtccgggt acgcgagatc gatcggggca aggtcagcct cgagctggtg
     1861 gaggcgctgg aaggcgccac tttgcccgat cccgaagccc cctcgggggg cggcggccgt
     1921 cgtgaaggcg gcggacggga
//
LOCUS       DATITW010000306         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_696259, whole genome shotgun sequence.
ACCESSION   DATITW010000306 DATITW010000000
VERSION     DATITW010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696259"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VLS86_04325"
     CDS             complement(<1..747)
                     /locus_tag="VLS86_04325"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSO49749.1"
                     /translation="MVLHAPGDLRLEEVQVPSPGGNELLLRVETVGICGTDASEYRHG
                     AKLFPLERRHPVTGHLGPMIPGHEFSGRVVAVGAGVEGFTTGELVVSAGSTACGSCHM
                     CRSKRPSRCDRYWAVGLQRDGALAEYCTAPANACLGVEAAGLTPDTAALAQPMSIAVH
                     ALRRARIEGGEEVVVIGAGGIGAFVTYAAARAGCSVVTVDLDRDRLAVAAGLGAGTTI
                     CVGSDATAGLAAGVVLEISGTEAGLRTALSM"
     gene            871..1776
                     /locus_tag="VLS86_04330"
     CDS             871..1776
                     /locus_tag="VLS86_04330"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HSO49750.1"
                     /translation="MEITNAERVVFPEDGITKGEVADYYALVAHRMLPFIEGRALTVQ
                     RFPRGIGGPGFMQKNAPDHYPDDLMGRHEVPKEEGGTTVYPVVDSVEGIIYFANLGVI
                     TFHVPPVRVVDEVHPDWAIWDLDPPPDRVGLVREAAAAMRATLEDHGIRTLPMASGSK
                     GYHLRARLEPGLTVETVALVARGTAALAAAAHDDLLTMAFRKAERGDRVFVDWMRNTT
                     RSTSVAPWSLRPRPGAPVAVPLTWEEVESVEPDGIGLRESESRLDFDPWAGEEQVDLG
                     PAVGTVEESLAAAGIELEPFDRFRS"
     gene            1802..>2733
                     /locus_tag="VLS86_04335"
     CDS             1802..>2733
                     /locus_tag="VLS86_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HSO49751.1"
                     /translation="MLLAELVTVSEAVAGTRSRSKKIDLLAEILRRLSPDEAPVAASY
                     LTGKPRQDRLGVGYATVFGVEVPPAAEADLKILQVDAILEAIARASGAGSKLRRESLL
                     TELLGRATAAEQNFLRGLILRNLRQGALEGVMADAVASAVGVGTDRVRRAAMLEGDLV
                     TVASRALTEGAESLGRSRLTLLTPVQPMLAKTAATAGEAVASIGTAIVEWKLDGARIQ
                     VHRERERVAVFTRNLRDITSGVPEVVAAISDLDSVSLILDGEILLLGANGSPLEFQDS
                     MSRFGSDGPQGGPALSAFYFDCLHLDGIDLIDAPL"
BASE COUNT          443 a          938 c          899 g          453 t
ORIGIN      
        1 catggataga gccgtccgca gaccggcttc ggtccccgag atctcgagga cgacacctgc
       61 cgccaacccg gccgtcgcgt cggaaccgac gcagatcgtg gtcccggctc cgaggccagc
      121 cgccaccgcg agacgatccc gatccaggtc gacagtcacc accgagcagc cggctctcgc
      181 cgcggcatag gtcacgaagg caccgatgcc cccggcgcct ataaccacca cctcttcccc
      241 gccctcgatc cgggcccgac gcagggcatg caccgcaatg gacatgggtt gggccagggc
      301 cgcagtgtca ggggtcaggc ccgccgcttc caccccgaga caggcattcg cgggagcggt
      361 gcagtattcg gccagggctc catcgcgttg cagcccgacc gcccaatacc gatcacatct
      421 gctgggacgc ttggagcggc acatgtgaca ggaaccacat gcggtggatc cggcgctgac
      481 caccagttca cccgtggtga agccctcgac tccggctccg acggccacca cccggccgct
      541 gaactcgtgg cccgggatca tcggccccag gtggccggtc accggatgac gtcgctccag
      601 gggaaagagc ttggcgccat gacggtattc ggaggcatcg gtgccacaaa tccccaccgt
      661 ctccacccgc agcaacaact cattaccacc cggagagggc acctggacct cctcgagacg
      721 gaggtcgcca ggggcgtgga gaaccaacgc ctgcatcgtc ggtgaagagg tcactccggt
      781 ggacactatt cggtgggttt tcgccctgtt cccggagcca ccgctccgcg attgacccct
      841 tgcctcgcaa ccacccataa cctgtcactc atggagatca cgaacgccga gcgcgtggta
      901 ttccccgagg acgggatcac caagggcgag gttgccgatt attacgcttt ggtcgcccac
      961 aggatgctcc ccttcatcga ggggcgggcg ctcaccgtgc aacgctttcc ccgggggatc
     1021 ggtgggcctg gcttcatgca gaagaacgcc cccgatcact atcccgatga cctgatgggc
     1081 cgtcacgagg tgcccaagga ggagggtggg accaccgtct acccggtggt cgactctgtc
     1141 gagggaatca tctacttcgc caacctcggc gtgatcacct tccatgtccc tccggtgagg
     1201 gtcgtcgacg aggtccatcc ggattgggcg atctgggatc tcgatcctcc gcctgaccgg
     1261 gtggggctgg tgcgggaagc cgccgcagcg atgcgagcca ccctcgagga tcatgggata
     1321 cgcaccttgc ccatggcgtc ggggtcgaag ggctatcacc ttcgtgcccg cctcgagccg
     1381 ggtctcaccg tcgagacggt ggccctcgtc gcacggggca ccgcggcact ggcggcagcg
     1441 gctcatgacg acctgctcac gatggccttc cgcaaggcgg aacgcggcga tcgggtgttc
     1501 gtcgattgga tgcggaacac gacccgttcc acctcggtcg ccccctggtc gctccgtccc
     1561 cgccccggcg ccccggtggc agtccccttg acctgggagg aggtcgagtc ggtcgagcct
     1621 gatgggatcg ggcttcggga gagcgaatcc cgtctcgact tcgatccctg ggcgggtgag
     1681 gagcaggtcg acctcggccc ggccgtgggc acggtcgaag agtccctggc cgccgccggg
     1741 atcgaactgg agccattcga ccgcttccgg tcctgacccg gagtctcggt accctccccg
     1801 ggtgctgctc gccgaactcg tgacggtgtc cgaagccgtc gccgggaccc gatcccgatc
     1861 gaagaagatc gatctgctcg ccgagatcct gcgccggcta tcacccgacg aggccccggt
     1921 tgccgcctcc tatctcaccg gaaagccccg ccaggatcgg ctcggtgtcg ggtatgccac
     1981 cgtcttcggg gtcgaggttc cccctgccgc tgaggccgat ctcaagattc ttcaggtcga
     2041 tgcgatcctg gaggcgatcg ccagggcctc cggggcgggc tcgaagctga gacgggagag
     2101 cttgctcacc gagctcctgg gccgggccac agctgcggag cagaacttcc tccgcgggct
     2161 cattctgcgc aaccttcgcc agggggcact ggagggggtg atggccgatg ccgtggcgag
     2221 cgcggtcggt gtgggcaccg accgggtgcg tcgggccgcg atgctggagg gggatctcgt
     2281 gaccgtggcc tcgcgggcgc tgacagaggg cgccgagtcc ctgggccgct cccggctgac
     2341 actgctgaca ccggtgcagc cgatgctggc caagacagct gccacggccg gtgaggcagt
     2401 ggcctccatc ggcaccgcca tcgtggaatg gaagctcgat ggagccagga tccaggtgca
     2461 tcgggagcga gaacgggtgg cggtgttcac caggaacctg cgagacatca cgtcgggagt
     2521 tcccgaggtc gtggccgcga tctccgatct cgattccgtc tcgctgatcc tggacgggga
     2581 gatcctcctc ctcggcgcga acgggtcgcc gctcgagttc caggactcga tgagccgctt
     2641 cgggtcggat ggcccccaag gcgggcctgc gctcagcgcc ttctacttcg attgtctcca
     2701 tctcgacggg atcgatctca tcgacgcacc gct
//
LOCUS       DATITW010000307         1549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_335536, whole genome shotgun sequence.
ACCESSION   DATITW010000307 DATITW010000000
VERSION     DATITW010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335536"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VLS86_04340"
     CDS             complement(<1..424)
                     /locus_tag="VLS86_04340"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004012685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HSO49752.1"
                     /translation="MARSIGILTAGGDSPGLNAAIRAVGKAAQRAYGMHVIGFRDGFR
                     GLMQNSFVRLDSDSLSGILTKGGTVLGTSRAKVDRMDMGDGRKMDMRPLMRDVVDQHD
                     LEALVVLGGDGTMRNALKLEDVGIRVLALPKTIDNDVTG"
     gene            complement(525..>1549)
                     /locus_tag="VLS86_04345"
     CDS             complement(525..>1549)
                     /locus_tag="VLS86_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HSO49753.1"
                     /translation="DWIRDLWASPPRPVDEDPGTVLLQLLDDPALGASNWVHEQYDHM
                     LFLNTVVGPGSDGSLIRIRGTEKGMAVSTDGNGRLCQLDPRAGGARLVYEAALNVAVT
                     GATPMAVVDNLNFGNPEKPFVMWQFRESVEGISEACESLGIPVVGGNVSFYNETEGVD
                     IHPTPVIGLLGLADPMPLPPPRISNARAGMEIWEVGPSPGVDLAGSAAQRVLHGELTG
                     RPTPPQPVAAGRVIALAAELAHLVPVLHDVSDGGRAVAIAEICIASGVGATVIDSEPA
                     EMFSEDPHRFIAVFETGVVELPGDISRRVGTMGGPVLTLGAAKPVDLDLLIETYRNAI
                     PRRMAG"
BASE COUNT          254 a          530 c          499 g          266 t
ORIGIN      
        1 tcccggtcac gtcgttgtcg atggtcttgg gcaacgcgag gaccctgatc ccgacgtcct
       61 cgagcttgag cgcgttgcgc atcgtcccgt caccaccgag caccaccagc gcctcgaggt
      121 cgtgttggtc gacgacgtcg cgcatcagag ggcgcatgtc catcttcctg ccgtctccca
      181 tgtccatcct gtcgaccttg gcccgactgg tgccgaggac agtgccgccc ttggtcagaa
      241 tcccggacag ggagtcggag tcgagacgca cgaacgaatt ctgcatcagg cccctgaacc
      301 cgtccctgaa cccgatgacg tgcatcccat aggcgcgttg cgctgccttg cccaccgccc
      361 gaatcgcggc gttcaacccg gggctgtcgc cccctgccgt gaggatgccg atgcttcgcg
      421 ccacgtggtg atggtatcgg ctccgattcg cagctcggac cagagccgtt ggttggggga
      481 acgaggcctc tttgtcgacg ccagccgagc gtcccgaccg ggactcagcc tgccatccgt
      541 ctcgggatgg cattgcgata ggtctcgatc aagagatcga ggtcgactgg cttcgcggca
      601 ccgagggtga ggacgggccc gcccatcgtc cccacccggc gactgatgtc gcccggcagt
      661 tcgacgacgc ccgtttcgaa gactgcgatg aagcggtggg gatcctccga gaacatctcg
      721 gccggctccg aatcgatgac ggtcgctccc acccccgatg cgatgcagat ctcggcgatg
      781 gcaaccgccc ggccaccgtc ggacacgtcg tggaggaccg gcaccaggtg ggccagttcc
      841 gcggcgaggg cgatcacccg ccccgcagcc accggttggg gcggcgtggg gcggccggtg
      901 agctctccgt gcagcactct ctgcgccgcc gagccggcga gatcgacgcc gggagacggc
      961 ccgacctccc atatctccat tcctgccctc gcattcgaga tacggggtgg gggcaggggc
     1021 atggggtcgg cgagaccgag aagcccgatc accggggtgg ggtggatgtc gacgccttct
     1081 gtttcgttgt agaaagacac gttgcccccg accaccggga tcccgagcga ctcgcatgcc
     1141 tcggatattc cctcgacgct ctcccggaac tgccacatca cgaagggctt ctccggattg
     1201 ccaaagttga gattgtccac caccgccatc ggtgttgccc cggtgacagc gacgttgagg
     1261 gcggcctcgt agacgagacg ggcacccccc gcccgtgggt cgagctggca aagacggccg
     1321 ttgccgtcgg tcgaaaccgc cattcccttc tcggtgcccc ggattcggat caacgaaccg
     1381 tcggagccag gcccgacgac cgtgttcaga aacagcatgt ggtcgtactg ctcgtgtacc
     1441 cagttcgagg cgccgagggc ggggtcgtcg agaagctgca atagcaccgt ccccggatcc
     1501 tcatccaccg gccggggtgg gctcgcccac aggtcccgaa tccagtcgg
//
LOCUS       DATITW010000308         1753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_576285, whole genome shotgun sequence.
ACCESSION   DATITW010000308 DATITW010000000
VERSION     DATITW010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576285"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1163)
                     /locus_tag="VLS86_04350"
     CDS             complement(138..1163)
                     /locus_tag="VLS86_04350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSO49754.1"
                     /translation="MAVRLESVDTSSVPESILSAMHDFYLEIDDEVLPGDPPKPYRQR
                     LVDWRHTPEFELVRHWVLWDDDRLVATSGAEMNLEQNLENAFGWLHVRAGERGRGLGR
                     IVATPMLDLVQEHNRTRFACALPQGSDAGAIAERAGMKNAFRDQRSRLNLAELDWNMV
                     DAWVARAPERASDYELIFLPPPIPDEYLQAYCDAHDIMNSAPMEDYEREPEVVTPEMW
                     RALEDFDRKRHTDFLVYAARHKPTGEIVGLTELVYQQLNPAQAWQADTGVHSDHRERG
                     LGRWLKAAMLQKVRSDYPLVERIDTDNAGSNAPMLSINLTLGFKPILVNHIWQGDVAT
                     IRERLEV"
     gene            complement(1209..1364)
                     /locus_tag="VLS86_04355"
     CDS             complement(1209..1364)
                     /locus_tag="VLS86_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49755.1"
                     /translation="MTNEAETAAASKRVMIVLVILFIVLVAAWAASSIDTIVAADEDA
                     SARLFRL"
     gene            complement(1457..>1753)
                     /locus_tag="VLS86_04360"
     CDS             complement(1457..>1753)
                     /locus_tag="VLS86_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSO49756.1"
                     /translation="DDGRPLAVGRRTRVIPNRLRRFILHRDGSCTGDGCTSRYRLEAH
                     HIVPWSEGGPTDPENLTTLCWFHHHVVVHGWGYRIDPRLGSGRLRFIKPGAGPP"
BASE COUNT          320 a          533 c          571 g          329 t
ORIGIN      
        1 ggcaccttgt tcgccgggcc cacggtgtgg cagggaaaga ccgcgatgag gatctcggtt
       61 tcctccttca agaccaccgg ggaggacgtg gagcgaagtg tccaggcgat cgtccgcctg
      121 gcgcgctcct gatcggtcta gacctccagc cgttcccgga tcgtcgccac atccccctgc
      181 cagatgtggt tgaccaggat cggtttgaac ccgagcgtga ggttgatgct caacatgggc
      241 gcgttggaac cggcgttgtc ggtgtcgatt cgctccacca gcggatagtc ggagcggacc
      301 ttctgcaaca tggcggcttt cagccagcga ccgagacctc tctcccgatg gtctgagtga
      361 actccggtgt ctgcctgcca ggcctgggcc ggattcagct gctgatagac gagctccgtg
      421 agcccaacga tttccccggt cggcttgtgc cgtgccgcgt acacgagaaa gtcggtgtgg
      481 cgtttgcgat cgaagtcctc gagggcgcgc cacatctcgg gtgtgaccac ctcaggctct
      541 ctctcgtagt cctccattgg cgctgagttc atgatgtcgt gggcatcgca ataggcctgg
      601 agatactcgt caggtatcgg cgggggcagg aagatgagct cgtagtcgga ggcccgctcg
      661 ggcgctcgtg ccacccaggc atcgaccatg ttccaatcca gctcggcgag gttgagacga
      721 ctcctctgat cgcgaaaggc gttcttcatc ccggcccgtt cggcgatggc gccggcgtcg
      781 gagccttggg gcaaggcgca ggcgaagcgg gtccgattgt gttcctgcac caggtcgagc
      841 atcggcgtgg cgacgatgcg gccgagtccc cggcctcgct cgcccgccct gacgtgcagc
      901 cagccaaagg cgttctccag gttctgctcg agattcatct cggctcccga ggtggccacc
      961 agccggtcgt catcccagag cacccagtgc ctgaccagct cgaactccgg ggtgtggcgc
     1021 caatcgacga gtcgctggcg atacggcttg ggcggatcac ccgggagcac ctcatcatcg
     1081 atctccagat agaagtcatg catcgcgctc aggatgctct cgggcacgct gctggtatcg
     1141 acggattcga ggcggacagc catgacgggt ggagaggcta cgaaggtgtc gcccgggaag
     1201 cggctgaatt acagacggaa cagacgagcc gaagcgtcct catccgcagc gacgatggtg
     1261 tcgatactcg atgctgccca cgccgcaacc aagacgatga acaggatcac gagaacgatc
     1321 atcacccgct tcgaagcagc ggcggtttca gcttcgttgg tcacgtccct ccgcgacggt
     1381 cgacgcaggg caggctaaac gccgatggtg ttatgaccag ggtttgttgg atgccggaaa
     1441 agacgaggct ctttgatcag ggagggccgg caccaggctt gatgaatctc agtcgaccgg
     1501 acccgagtct ggggtcgatc cgatatcccc agccatggac gaccacatgg tgatggaacc
     1561 aacaaagtgt ggtcaggttc tcgggatcgg ttggccctcc ctcggaccaa ggaacgatgt
     1621 gatgggcctc gagccggtat cgactggtgc atccgtcccc ggtgcacgac ccgtcgcgat
     1681 ggaggatgaa gcgacgaaga cggttcggga tcaccctcgt tcggcggcct acggcgagcg
     1741 gccgaccatc gtc
//
LOCUS       DATITW010000309         3217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_536255, whole genome shotgun sequence.
ACCESSION   DATITW010000309 DATITW010000000
VERSION     DATITW010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536255"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..485
                     /locus_tag="VLS86_04365"
     CDS             99..485
                     /locus_tag="VLS86_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HSO49757.1"
                     /translation="MSPDRGTATILILGLGALVATIGIATAALGFLFDAREQAATAAE
                     AAALAAAVATYPPTGAGLPETLAAEYASRNGARMVSCWCPVDGALRARVVAVTVSLSA
                     DLPVFGEVLVGKTARAEFDPRLWLGR"
     gene            498..980
                     /gene="ybaK"
                     /locus_tag="VLS86_04370"
     CDS             498..980
                     /gene="ybaK"
                     /locus_tag="VLS86_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HSO49758.1"
                     /translation="MSTTATRATEFLDRAGVTYRAHRYEVTEKVGDGYGEAVADAIGL
                     PGSRVFKTLVAEVDQEPVVAVIPVDRRLSTKQLARIVGGKRCSLASPETAERLTGYVT
                     GGISPFGQRRKLRLILDTSASEFETIAVSGGRRGLQLELEPGVLLDLTGGTMGAVAER
                     "
     gene            1003..2733
                     /gene="polX"
                     /locus_tag="VLS86_04375"
     CDS             1003..2733
                     /gene="polX"
                     /locus_tag="VLS86_04375"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HSO49759.1"
                     /translation="MATRLETLRMLAELAKLTALDEGSPQAFRVRAYENALHGIEGYQ
                     GELSGLSKQQLVEIKGVGASTADKILEFHSTGSVAKLDALREKYPPAFVELTKIPGLG
                     PKTLKLLRSELGVEDIAGLREAISTERLRELPGLGKTSEEKIGKAIERLGLHGKDRRS
                     ALVEALPLARSLAARITSLTSVEEAVPCGSIRRFSETVGDIDIVVATTDPAQVSAFVL
                     GLPEVHEVIGSGDTKTSFLTREGMQVDVRTVRPDQLGSALLYFTGSKAHNIALRQRAI
                     DRGWLLSEYGLFEEDTVVASHTEESVYEALEMAFVPPPLREGSGEVEAAATGDLPALV
                     ERSQIKGDLHYHSDRSGDGRSSLEEMVEAAIARGFEYVAFTDHGEDLAINGSSREVML
                     AHRDRIRELQVAYPQIRLLFGCELNIGPDGSLDYDPDFRLEFDYCVASIHSHFDLPQD
                     RQTARVLTALGDPSVNAIGHLTGRYIGRRPGVDLDVEVILEALEVTGVALEINGALDR
                     LDASSEVARQAMARGVDVVIDTDSHHTSELVRMDYGVAYAQRGWVTSDRVVNTRPVDE
                     FMEWVQRRRG"
     gene            2743..3174
                     /locus_tag="VLS86_04380"
     CDS             2743..3174
                     /locus_tag="VLS86_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HSO49760.1"
                     /translation="MAKRYEVTISTLRVNRFTSWIARLGVGRTEVLTTTGRHSGEPRN
                     VPVSPIAVDGVEYVVSPYGEVGWVHNVRADPVVSLRHGSKRRAARLEEVTGPAAAPVV
                     AAYHAREGYARPYMDVPENPGLDDFVTAAGEFPVFAVVAIT"
BASE COUNT          539 a         1035 c         1089 g          554 t
ORIGIN      
        1 tcctcggtga cggtctcgtt gcgccatgcc ttgggtcgac ccttcccgac ctccataggg
       61 gtcgacctgt cggctcgggc cacgatgcga accgagagat gagccccgat cggggcaccg
      121 ccaccatcct gatcctgggg ctgggcgctc tggtcgccac gatcggcatc gccaccgcag
      181 ctctcgggtt tctgttcgac gcccgggaac aggcggcgac ggcagccgag gctgccgccc
      241 tcgccgctgc agtggccacc tacccgccga cgggcgccgg gctgcccgag accctggccg
      301 cggagtacgc ctcacgcaac ggagctcgga tggtgagttg ctggtgcccg gtcgatggcg
      361 ccctccgcgc ccgagtcgtt gcggtgaccg tatccctctc cgccgatctc cccgtgttcg
      421 gcgaggtcct ggtgggcaag acggcccggg cggagttcga ccctcgcctc tggctcggtc
      481 gatgacacaa ttgcccggtg tcgaccactg ccaccagggc caccgagttt ctcgaccggg
      541 cgggagtgac ctatcgcgcc catcgctatg aggtgaccga gaaagtaggt gatggatacg
      601 gggaagcggt ggccgacgcc atcggccttc ccgggtcgag ggtgttcaag accctcgttg
      661 ccgaggtcga tcaggagccg gtggtcgcgg tgatcccggt cgatcggaga ctgtcgacca
      721 agcaacttgc caggatcgtc gggggaaagc ggtgctcgct ggcgtcacct gagaccgcgg
      781 agcgtctcac cggatacgtg accggaggca tcagcccgtt tgggcagcgg cggaagctcc
      841 gactcatcct cgacacatcg gcgtcggagt tcgagacgat cgcggtgtct ggcgggcgca
      901 ggggactcca gctcgaactg gagcccgggg tcttgctcga cctcaccggt gggacgatgg
      961 gggccgtggc tgagcgctga tcctgagatc taacctcggc agatggccac ccgactcgag
     1021 acgctgcgaa tgctcgccga gctggccaag ctcaccgccc tcgacgaagg gagcccgcaa
     1081 gcgttccggg tgcgcgccta cgagaacgcc ctgcatggga tcgagggcta ccagggcgag
     1141 ttgagcgggc tcagcaaaca gcagctggtc gagatcaagg gggtcggggc gtcgacggca
     1201 gacaagatcc tggagttcca cagcacaggg tcggtggcca agctcgacgc gctcagggag
     1261 aaatatcccc cggccttcgt cgagctgacc aagatccccg gtctcggtcc caagacactc
     1321 aagttgctcc ggtccgagct cggcgtcgag gacatcgctg gtctgcgcga ggccatatcc
     1381 accgagaggc tccgcgagct gcctggtctg ggcaagacct ccgaggagaa gatcggcaaa
     1441 gccatcgagc gacttggttt gcatggcaag gaccgccggt ccgcattggt cgaggctctt
     1501 cctctcgccc gctctctggc tgcccggata acgagtctga cgtcggtcga ggaggcagtg
     1561 ccctgtgggt cgatccggcg attctccgag acggtggggg atattgacat cgtggtggca
     1621 accacagacc cggcccaggt ctccgcattc gtgctgggcc tgcccgaggt gcacgaggtc
     1681 atcggctccg gggacaccaa gacctcgttt ctcacccgtg aaggcatgca ggtcgacgtc
     1741 aggaccgtgc ggcccgatca gctcgggtcg gccctgctct atttcaccgg ttccaaggcg
     1801 cacaacatcg cacttcgcca gcgagcaatc gaccgtggtt ggctgctcag cgagtacggc
     1861 ctgttcgagg aggacacagt cgtggcctca cacaccgagg aatcggtgta cgaggcgctg
     1921 gagatggctt tcgtcccacc cccgctccgg gaggggtcgg gcgaagtgga ggcggcggcc
     1981 acgggcgatc tgccggcgtt ggtggagcgg tcacagatca agggagacct ccactaccac
     2041 tccgatcgtt ccggcgatgg ccgttccagc ctcgaggaga tggtcgaggc ggcaatcgcc
     2101 cgaggcttcg agtacgtggc gttcaccgac cacggcgagg acctggcgat caacgggtcg
     2161 tcccgtgagg tgatgctcgc ccatcgcgat cgaatcaggg agctgcaggt cgcatatccc
     2221 cagatccggc tcctcttcgg ctgtgagctc aacatcggcc cggatgggag tctcgactac
     2281 gaccccgact tccgtcttga gttcgactac tgcgtcgcct cgatccactc ccatttcgac
     2341 cttccccagg accggcagac ggcgcgagtg ctgaccgcgc tcggcgatcc gtccgtgaac
     2401 gccatcgggc acctcaccgg ccgatacata ggtcgtcggc ccggcgtcga cctcgatgtt
     2461 gaggtgatcc tcgaggcatt ggaagtgacc ggagtggccc tcgagatcaa cggcgccctg
     2521 gatcgtctcg atgcctcctc cgaggtggcc cgccaggcaa tggccagggg agtggacgtg
     2581 gtcatcgaca ccgactcgca tcacaccagc gagctggtcc ggatggacta cggagtggcc
     2641 tatgcccagc gtggctgggt cacctcggat cgggtggtga acacgcgtcc ggtcgatgag
     2701 ttcatggagt gggtccagag gcggaggggc taggttccgg ggatggcgaa acgctacgaa
     2761 gtcaccatct ccacgctcag ggtcaatcgt ttcacgtcct ggattgcgcg actgggtgtg
     2821 ggcaggaccg aggtgctgac caccaccggt cgccacagcg gggagccacg caacgtgccc
     2881 gtcagcccga tagccgtcga tggggtggag tacgtcgtct ccccatacgg agaggtgggt
     2941 tgggtccaca acgtcagggc cgaccctgtg gtctcgttgc gtcacggatc gaaacgacgt
     3001 gcggcgcgtc tggaggaggt caccggcccc gcagccgccc cggtcgtcgc ggcctatcac
     3061 gcccgcgagg gatacgcccg gccgtacatg gacgtccccg agaaccctgg tctcgacgac
     3121 ttcgtgaccg cggccggtga gttcccggtg ttcgccgtgg tcgccatcac gtaattgaga
     3181 cggaccgggg tcatatccgg acctgtggct cccaatt
//
LOCUS       DATITW010000310         2712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_135256, whole genome shotgun sequence.
ACCESSION   DATITW010000310 DATITW010000000
VERSION     DATITW010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135256"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..2310)
                     /locus_tag="VLS86_04385"
     CDS             complement(22..2310)
                     /locus_tag="VLS86_04385"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HSO49761.1"
                     /translation="MSPEHVTRSILVATAVGLVVGGVLWFAGQETAANVVFVLVAVIP
                     VIPLVRDIAAKLRRGQPGVDLIALVAVAAALALGEYLTAAIVGLMLATGQFLEEFAAG
                     RAERELTALVKRAPRWANRVKDGEIETVDVESVVRGDRLLIMAGEVVPVNGVILSDFA
                     LVDESALTGEPLPVERRGGDLVSSGSVNGADVFEIQATADSEHSTYAGIIRLVQEARE
                     SRAPSVRLADRWAAWFVPLALGVSGLAWAVSGDPVRGLAVLVVATPCPLLLAVPIAVV
                     SGISRAARRGIVFRGGGSLETLAQTQNVLIDKTGTVTIGEPSLRGLVTFGEATQDDAL
                     RFAASVDQTSTHVLARAIVGAARHRDLRLEMPSDVIEVAGSGVSADVDGHRVGVGRLE
                     WLLDGRPEPEAVAEFRRRMLRVAPLAVFLAIDGEVAAALTFDDTIRPDAANTIRALRR
                     TGVRRILMATGDHPVVARSVGLAVDVDDVLAECTPREKVEAVHQLRGQGVTTMVGDGI
                     NDAPALAAADVGVAMGARGATASSEAADVVLMVDQLQRLVDAIGIAQRSRRIAVQSVV
                     IGMGLSLGAMAFAFFGWLTPIAGALVQEGIDVIAITNALRALVGKTPSQTGPKLSPEL
                     SRRLRREHDELMPRLDALRDTADMLDDISPSEALKALEEIRRLLVDEILPHEAEDEKV
                     IYPQLAAILAGEDPMALMSRTHREIFHLVDVYQRQLTDLPASGPEPADIRDLRRTLYG
                     LHAILRLHFDQEEELYFSLQDA"
     gene            complement(2374..>2712)
                     /locus_tag="VLS86_04390"
     CDS             complement(2374..>2712)
                     /locus_tag="VLS86_04390"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HSO49762.1"
                     /translation="AGIITLEDIAEELVGEISDEHDSEQQRVSIDASGRILAAGTVRP
                     DELSRHGIRLPGGDYETIGGLIMNRLGRLPRRGDVIEDDGWRMRVVKTDGRRVGEVEV
                     TPLDEGPSAG"
BASE COUNT          465 a          941 c          889 g          417 t
ORIGIN      
        1 ggcccaattc gcgccttgcc tctaggcgtc ctggagcgag aagtagagct cttcctcctg
       61 gtcgaagtgc agacggagga tcgcgtgcag gccgtacagg gtgcgccgca ggtcgcggat
      121 gtctgcaggc tcggggccgg aggcgggcag atcggtcagc tggcgctgat acacgtcgac
      181 gagatggaag atctcgcggt gagtgcggct catcagcgcc atcgggtcct caccggccag
      241 gatcgcggcc agctggggat agatcacctt ctcgtcctca gcttcgtggg ggagtatctc
      301 gtccaccaag agccggcgga tctcctcgag ggcctttagg gcttcggatg gcgagatgtc
      361 atcgagcatg tcggccgtgt cccgcagagc gtcgagccgc ggcatcagct cgtcgtgctc
      421 gcggcgcagt cgacgggaga gctccgggga gagcttgggg ccggtctgcg atggtgtctt
      481 ccccaccagc gcccgcagcg cgttggtgat cgcgatcacg tcgatgccct cttgaaccag
      541 tgcgccggcg atgggcgtga gccagccgaa gaaggcaaac gccatcgcgc ccagggaaag
      601 acccattcca atgacgacgc tctggaccgc tatccgtctc gacctctggg cgatgccgat
      661 ggcgtcgacg agacgttgca gttgatcgac catgagcact acgtcggccg cttcggagga
      721 ggcggtggcg ccccgggctc ccatggccac gccgacatcg gcggcggcca gagccggagc
      781 atcattgatc ccgtccccaa ccatcgtggt caccccctgg cctcgcagct gatgcaccgc
      841 ttcgaccttc tcccttgggg tgcattcggc gaggacatcg tcgacatcga ccgccagccc
      901 caccgacctg gcaacgaccg gatgatcgcc ggtggccatg aggattcgac gaacgccggt
      961 gcgacgcaga gcgcggatgg tgttggcggc gtcggggcga atggtgtcgt cgaaggtgag
     1021 cgccgctgcc acctcaccgt cgatggcgag gaacaccgcc agtggcgcga cacggagcat
     1081 gcggcgtcgg aactcggcaa ccgcttcggg ctccgggcgg ccatcgagca gccactccag
     1141 cctgcccacg ccgaccctgt ggccgtcgac atcggcgctc acccccgatc ccgcgacctc
     1201 gatgacatcg gaaggcatct ccagacgcag atcacggtgc cgcgccgcgc cgacgatggc
     1261 tcgggccagg acgtgggtgg acgtctggtc gaccgaggct gcgaagcgaa gagcatcgtc
     1321 ttgtgtggcc tcgccgaagg tgacgagacc gcgcagcgac ggctctccga tcgtgaccgt
     1381 ccccgtcttg tcgatgagga cgttttgggt ctgggcaagg gtctcgagcg agccaccacc
     1441 ccggaacacg atcccgcgtc gggcggcgcg cgagatcccc gacaccaccg cgatcggaac
     1501 cgccagcagc aacgggcaag gggtggcgac aaccagcacc gcgagaccac gcaccggatc
     1561 accggacact gcccaggcca gaccggagac gccgagggcg aggggcacga accatgccgc
     1621 ccatcggtcg gcgagacgca ccgatggggc gcgggactcg cgtgcttcct ggaccagtcg
     1681 aatgatcccg gcgtaggtgc tgtgctcgga gtcggccgtt gcctggatct cgaagacgtc
     1741 ggcgccgttg accgaaccgc tgctgaccag atcaccgcct ctgcgctcga ccggcaaggg
     1801 ctccccggtc agcgccgact cgtcgacgag cgcgaagtcg ctgagaatca ccccgtttac
     1861 gggaaccacc tctccagcca tgatcaggag ccggtcgccc cgcaccaccg actcgacgtc
     1921 caccgtctcg atctcaccgt ccttgacccg gttggcccag cgaggggccc gcttcaccaa
     1981 ggcggtgagc tcgcgctctg cccttcccgc ggcgaactcc tccagaaact gcccggtggc
     2041 gagcatgagc ccgacgatcg ctgcggtcaa gtactccccg agggccagcg ccgccgccac
     2101 cgccaccaac gcgatcaggt ccaccccggg ctgaccacgc cgaagcttgg ccgcgatgtc
     2161 acggaccagc gggatcaccg ggatcacggc gaccaggacg aagacgacgt tggccgcggt
     2221 ttcttgcccg gcgaaccaga gcacgcctcc cacgaccagc cccactgcgg tggccaccag
     2281 aatcgagcgg gtgacgtgtt cggggctcat ctctcgcagt taagcctcag acactcggga
     2341 ccgaatagag gatgtcgacc cggatctctg ggatcaaccg gcggaagggc cctcgtcgag
     2401 cggcgtgacc tcgacttcac ccactcgccg accatcggtc ttgacgaccc tcatcctcca
     2461 accgtcgtcc tcgatcacgt cgccgcgacg cgggagccgc ccgagacggt tcatgatcag
     2521 gcccccgatg gtctcgtaat cgccgccggg aagcctgatc ccgtgtcggg agagctcgtc
     2581 gggccgcacc gtgcccgccg ccagtatccg gccggaggcg tcgatggaca ctcgctgctg
     2641 ctcactgtcg tgctcatcgg atatctcccc gaccagctcc tcggcgatgt cctcgagggt
     2701 gatgatgccg gc
//
LOCUS       DATITW010000311         1849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_455915, whole genome shotgun sequence.
ACCESSION   DATITW010000311 DATITW010000000
VERSION     DATITW010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455915"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /gene="folE"
                     /locus_tag="VLS86_04395"
     CDS             complement(<1..477)
                     /gene="folE"
                     /locus_tag="VLS86_04395"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HSO49763.1"
                     /translation="MGDIDQARIEKAVQEIIEALGEDAGREGLVDTPQRVAKFYAEVF
                     DGLHRDPGDVVDAFFGEEHYQEIVMVREIPFYSMCEHHFVPFHGQAHVAYMPRGRVTG
                     LSKLARLVDGFARRPQMQERLTAQVADALNERLDPLGVLVVIEAEHLCMSMRGVKKP"
     gene            688..1149
                     /locus_tag="VLS86_04400"
     CDS             688..1149
                     /locus_tag="VLS86_04400"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HSO49764.1"
                     /translation="MQLNRVAGRLETAVEAQLRLGGPEVADLGSELLEVLRPAMRQTL
                     MEVVEMAAQEINGQLVGQRVDVRLVDGDPELTVQPDQSGIPSPPTPPGVDEPMDAEAR
                     ITLRLPGYLKDLIAAAADGAGDSVNSFVVDALRSRAYERVGATSVKRTIEL"
     gene            1146..>1849
                     /locus_tag="VLS86_04405"
     CDS             1146..>1849
                     /locus_tag="VLS86_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HSO49765.1"
                     /translation="MIESDFSVGDSAMMRIAIRSGRIDVEPGEAGVVQVTVDTRDRTF
                     EVRQRGDTIVASGKKGDRAHVTVRVPPTTDIDASTTSGDVNVGSPVRHLDVVSASGNV
                     LFGSVERLQTRSSSGSIRGDSVAGEARCETASGDIRIAQILERGDLATASGNIAVDLC
                     AGTISCSTVSGNVRLNVVTGPSLTIKSTSGLVRVGIPSGTRLDLDANTFSGRVTLPAP
                     HPHPEPAEREITARVRL"
BASE COUNT          345 a          587 c          603 g          314 t
ORIGIN      
        1 gggcttcttc actcccctca tcgacatgca gagatgctcg gcctcgatca cgaccagcac
       61 ccccaggggg tcgagacgct cgttgagcgc atcggcgacc tgggcggtga gacgttcctg
      121 catctggggg cggcgggcga atccatcgac gagccgggcc agcttggaga gaccggtgac
      181 ccgtcccctc ggcatgtacg ccacatgggc ctggccgtgg aaagggacga aatgatgctc
      241 gcacatcgag tagaacggga tctcgcggac catcacgatc tcctggtagt gctcttctcc
      301 gaagaaggcg tcgactacgt cgcccgggtc gcgatgcagc ccgtcgaaca cttcggcgta
      361 gaacttggcc acccgctgcg gcgtgtcgac cagaccctcg cgcccggcat cctcgcccaa
      421 tgcctcgatg atctcttgaa cggctttttc gatgcgagct tggtcgatgt cacccatcgc
      481 cagaggaaat cgaccggaac gctgtttgta ttccccaaat cgcgcaatgg gttgacatcg
      541 gtggcgccat gacattatga tgacatcatt ctgacatcat caaagatgtc gactaccgga
      601 gaatgccccc atgttgtaca tccactccaa agacagtctc gaggcggtcc tcgaagaacg
      661 gatccgtgag ataaggaagg cgaggggatg cagctgaacc gagtcgcagg cagactggag
      721 accgcggtcg aggcacagct gcgtctcggt ggtcccgaag tggccgatct cggatccgaa
      781 ctactcgaag tgctccgtcc cgcgatgaga cagaccctga tggaggtggt cgagatggcc
      841 gcccaggaga tcaacggcca actggtcggc cagcgggtcg acgttcggct ggtcgatggt
      901 gaccctgagc tcacagtcca gcccgatcaa tcgggcatcc cttccccgcc gacacctccc
      961 ggggtggacg agccgatgga cgccgaggcg aggatcacgc tccgcctccc tggctatctc
     1021 aaggacctga tcgccgcggc cgccgacggt gccggcgact cggtcaactc gttcgtggtc
     1081 gatgctctcc ggtcgagagc ctacgagcgg gtcggggcca cgtccgtgaa gaggacgatc
     1141 gagctttgat cgagagcgac ttctccgtcg gggactccgc catgatgcgg atcgccatca
     1201 ggtcgggtcg catcgacgtc gaacccggtg aggccggtgt ggtccaggtg accgtcgaca
     1261 cccgtgaccg cacattcgag gtacggcagc ggggggacac gatcgttgcc tccgggaaga
     1321 agggggaccg ggctcatgtc acggtgagag tgcctccgac gaccgacatc gacgcctcga
     1381 cgacgagcgg tgacgtgaac gtcggctccc cggttcgtca cctcgacgtg gtgagcgcca
     1441 gcgggaacgt cctcttcggc tcggtggaga ggctgcagac caggtcctcg agcgggagca
     1501 tcaggggtga cagcgtcgca ggagaggcga gatgcgagac cgcgtcgggg gacatccgca
     1561 tcgcacagat cctcgagcgc ggcgacctgg ctacggcctc ggggaacatc gccgtcgatc
     1621 tctgtgcggg gacgatctcc tgttccacgg tctcggggaa tgtccggctc aacgtggtca
     1681 ccgggccatc gctgaccatc aaatcgacgt cggggctggt ccgggtcggg atcccgtcgg
     1741 ggaccaggct cgacctcgac gccaacacct tctcgggccg ggtgaccctt cctgcccccc
     1801 acccgcaccc cgagccagcg gaaagggaaa tcaccgccag ggtgcgtct
//
LOCUS       DATITW010000312         1967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_255575, whole genome shotgun sequence.
ACCESSION   DATITW010000312 DATITW010000000
VERSION     DATITW010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255575"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..515)
                     /locus_tag="VLS86_04410"
     CDS             complement(138..515)
                     /locus_tag="VLS86_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49766.1"
                     /translation="MLEYFIGFSMGQQSAARAASLARSAAVADGTHHTNRIEDLNERI
                     DKLLMIVRAMWGLLEEQGYTAESLSAKLEEIDLEDGVLDGQVTPSLVECPSCQSKVAP
                     GLLNCQLCGTAVRAEAGHPLGQI"
     gene            complement(560..>1967)
                     /locus_tag="VLS86_04415"
     CDS             complement(560..>1967)
                     /locus_tag="VLS86_04415"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HSO49767.1"
                     /translation="SAWAKGQPTSFPGWLPWVASMTMAMAILGAFTEYAHPAIDTWPE
                     ATVSLDVDRSDLVLVSVASRNQTRIHVDGSDRVWMPDFAPDGRIALTVVADEVGRLVT
                     MNVDGSDPRTLHEGDSFFHHAAWSPDGSMIAFSTETDGQVDLFLMPGDGGEPTRLTDD
                     EFVDWGPSWSPDGSRIVFVSDRDGSPGLNLVSSAGGEPTRLTDDPGFEGAPAWSPDGE
                     WIAFETNASGSSEIALIRPDGSEEVTLTADDASDVAPEWSPDGSRIVFASDRGGEIDL
                     YVMARDGSGVEAVAANPGAQDGWAGSSWSDDAEIIATNQSGNTPYWLEPDVRQMLGVA
                     ALLIQAILISGFVLLVLRHGRIPLGSITVMVGVSGAMMTVISDNYWYIAVAVAAGVVG
                     DVIAVSTNPTPEQPRSIRPLAFLLPAVWYSFYLLALAVFGEGIGWSVHMTVGAPLLAG
                     AAGLLLSLLVFPAPSATR"
BASE COUNT          340 a          663 c          645 g          319 t
ORIGIN      
        1 aggctggccg aggtcatggg cggagggatc acctcggtca gtgtgggagg ggcaaccgcg
       61 atccacgtcc tgttgcccag gacctatttc gctgccgacc cggcggagcg tcagaccgca
      121 aagacgacgc tgagcgccta gatctggccc agcgggtgcc cggcttcggc acggacggcg
      181 gtgccgcaca actggcagtt caacagcccg ggagccactt tggactgaca cgacgggcac
      241 tccaccagtg aaggtgtgac ctggccgtcg agcaccccgt cttcgagatc gatctcctcg
      301 agtttggcgc tcagcgattc ggcggtgtag ccctgctctt cgagcagccc ccacatggcg
      361 cgcacgatca tcagcagctt gtcaatccgc tcgttgagat cctcgatccg gttggtgtgg
      421 tgggtgccgt cggccaccgc cgcgctgcgc gccagagagg cggccctggc cgcgctctgc
      481 tgacccatgc tgaacccgat gaagtactcg agcatggctc agatatcggt gatgagagct
      541 ttcgactcaa gacccggatt cagcgggtcg ccgagggcgc cgggaagacg agcaggctca
      601 acagcagacc ggcggccccg gccaggagcg gggcgccgac cgtcatgtgc accgaccagc
      661 cgatgccctc gccaaacacc gccagggcga gcaggtagaa ggaataccag accgcgggca
      721 ggagaaaggc cagaggcctg atcgatctcg gctgctccgg cgtgggattg gtcgagaccg
      781 caatcacgtc ccccaccacc ccggcggcca ccgctactgc gatgtaccag tagttgtcgc
      841 tgatcaccgt catcatcgcc ccgctgactc cgaccatgac cgtgatcgat cccaggggga
      901 tccgtccgtg gcgaagcacg aggagcacga acccgctgat caggatggcc tggatgagga
      961 gtgcggccac gcccaacatc tgcctgacgt ctggttcgag ccaatacggg gtgttgcccg
     1021 actggttggt ggcgatgatc tcggcgtcgt cggaccagct ggagcccgcc cacccgtcct
     1081 gcgccccggg gttcgccgcc accgcctcca cgcccgatcc gtctcttgcc atcacgtaca
     1141 ggtcgatctc gccgccacgg tcgctggcga agacgatccg tgatccgtcc ggcgaccatt
     1201 ccggcgcaac gtcggaggcg tcgtccgcgg tgagggtgac ctcctccgat ccgtcgggtc
     1261 ggatcagggc gatttcggaa ctgccgctgg cgttggtctc gaacgcgatc cattcgccgt
     1321 cgggcgacca ggcgggcgcc ccttcgaagc ccgggtcgtc cgtcaaccgg gtcggctcgc
     1381 cacccgccga cgagacgaga ttcaaacccg ggctcccgtc ccggtccgag acgaagacga
     1441 tgcggcttcc gtccggtgac caggaaggtc cccaatcgac gaactcgtcg tcggtgagac
     1501 gggtcggttc gcctccgtcg cccggcatca ggaacagatc cacctggccg tcggtctcgg
     1561 tggagaaggc gatcatcgat ccgtccgggg accaggcggc gtggtggaag aagctatcgc
     1621 cttcgtgcag tgtcctcggg tcggagccat cgacattcat cgtcaccaga cggccaacct
     1681 cgtcggcgac cacggtcaac gcgatacgcc cgtccggggc gaaatcgggc atccacaccc
     1741 ggtccgaccc gtcgacgtgg atccgggtct gattccggct ggcgaccgag accaggacca
     1801 ggtctgaccg atccacgtcg aggctgaccg ttgcttcggg ccaggtgtcg atggcgggat
     1861 gggcgtactc ggtgaacgct ccgaggatgg ccatcgccat ggtcatcgag gccacccatg
     1921 gcagccatcc cggaaacgac gtgggttgcc ctttggccca cgccgag
//
LOCUS       DATITW010000313         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_15534, whole genome shotgun sequence.
ACCESSION   DATITW010000313 DATITW010000000
VERSION     DATITW010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15534"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="VLS86_04420"
     CDS             <1..175
                     /locus_tag="VLS86_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HSO49768.1"
                     /translation="VIHTDFQRGFLAAEVADFGRLVEAGSWIAARAKGLVRTEGKSYV
                     MRPDDVVEFRFNV"
     regulatory      215..302
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            356..>2254
                     /locus_tag="VLS86_04425"
     CDS             356..>2254
                     /locus_tag="VLS86_04425"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784296.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HSO49769.1"
                     /translation="MPNPRERFADRHIGPRSDDIEKMLATIGAATLDEMVSETIPASI
                     LDTTLDLPDPMSEQETIGKLRSFASQNTVLTSLIGLGYHDTITPPVIQRNVLESPAWY
                     TAYTPYQPEISQGRLEARLNFQTRVSDLTGLEVANASLLDEATAAAEAMAMCRRLTKG
                     DRSVFYVDDDCHLPTTAVVETRAGAAGVEIQRLSEGFEPDRAFGLLLQYPGSSGAIRD
                     HRELVAEARAAGVFVVVATDLLALTLLAPPGEWGADIVVGSAQRFGVPLGYGGPHAGF
                     IATNEAHVRALPGRLVGVSHDRSGRVALRLALQTREQHIRREKATSNVCTAQVLLAVI
                     AGFYGCWHGPDGLREIAGEVHRLTGLVASSLTGAGFELVSGTFFDTVTVKAPGRAREI
                     LALALAGGVNLGMVDDDHLRIALDETTTPGIVHQVLAAFGAKAADDGGSTGLPAGLTR
                     RTEFMTHPVFSLHRSEHEMLRYLRRLSDRDLALDRSMIPLGSCTMKLNATTEMIPMTW
                     PEFAGIHPYAPDAQAAGYHALIDELSDYLCRITGYDAVSVHPNAGSQGELAGLLAIRA
                     YHRSRGDSDREICLIPSSAHGTNAASAVMAGMKVVVVATDESGNVDLADLEAKASEHA
                     DRLAAAMIT"
BASE COUNT          376 a          771 c          739 g          368 t
ORIGIN      
        1 ggtgatccac accgacttcc agcgtgggtt cctcgccgcc gaagtggccg atttcggtcg
       61 tctcgtcgaa gccggctcat ggattgccgc ccgggccaaa ggcctggtcc ggaccgaggg
      121 caagtcctat gtgatgcgtc ccgacgacgt ggtcgagttc cgcttcaacg tgtgaccgat
      181 cagcgcggtt ccggacctat gattacccgg ccatcgacac cgaatgggag agccccgcac
      241 gggggcgccg aaggggcaac tcctccccgg aaactctcag gcacgtggac cattcggtcg
      301 aggcgccgct gaagggaaca acagcggggg agggcattcc gacccgagga gacagatgcc
      361 caaccccaga gagcgcttcg cagaccggca catcggcccc cggtccgacg acatcgagaa
      421 gatgctggcg acgatcggcg ccgcgacgct ggatgagatg gtgtcagaga ccatcccggc
      481 ctcgattctc gacaccacgc tcgatctccc ggatccgatg tccgagcagg agacgatcgg
      541 gaagctgcgc agcttcgcct cgcagaacac ggtgctcacc tcgttgatcg ggctcgggta
      601 tcacgacacg atcacgccac cagtgatcca gcgcaacgtc ctggaaagcc ctgcctggta
      661 cacggcctac accccgtacc agcctgagat ctcccagggt cgcctcgagg cgcgtctcaa
      721 cttccagacc agggtctcgg acctgacggg tctcgaggtg gccaacgcct ctctgctcga
      781 cgaggcgacc gcagccgccg aggcgatggc gatgtgtcgc cgactgacca agggcgaccg
      841 gtcggtgttc tacgtcgacg acgattgtca tctccccacc accgccgtgg tcgagacccg
      901 agccggggcg gcgggagtgg agatccagag actgtcggag ggattcgaac cggatcgggc
      961 gtttggcctg cttctccagt accccgggtc ttcgggagcg attcgagacc accgggagct
     1021 ggtcgcagaa gcgcgggcgg caggagtctt cgtcgtcgtc gccacggacc tgctcgcctt
     1081 gaccttgctc gccccacccg gggagtgggg tgcggacatc gtcgtcggct cagcgcagcg
     1141 ctttggcgtc ccgcttggct acggcggccc ccacgccggg ttcatcgcca ccaatgaagc
     1201 tcatgtccgc gcccttcccg ggcggctggt cggggtctcc cacgacagat cgggtcgtgt
     1261 cgccctccgc ctcgccctgc agacccgtga gcagcacata cggcgggaga aggccacctc
     1321 caacgtgtgc accgcccagg tgctgctggc cgtgatcgcc ggcttctacg gctgctggca
     1381 cggccccgac gggttgaggg aaatcgccgg tgaggtgcat cggctgaccg ggctggtcgc
     1441 ctcctcgctg accggcgccg ggttcgagct ggtcagcggg acattcttcg acaccgtcac
     1501 ggtcaaggcc ccggggcgag cgcgggagat cctcgccctg gcgctggccg ggggagtcaa
     1561 cctcgggatg gtcgacgacg accacctccg gatcgcactc gacgagacca ccacgcccgg
     1621 gatcgtgcac caggtactcg ccgccttcgg ggcgaaggcg gcggacgacg gcggctcgac
     1681 cgggcttccg gcggggttga ccagacgtac ggagttcatg acccatccgg tcttcagcct
     1741 ccaccgatcc gagcacgaga tgctccgcta tctgcgccgt ctctcggatc gcgacctcgc
     1801 cctggaccgg agcatgatcc ccctcgggtc gtgcacgatg aagttgaacg cgaccaccga
     1861 gatgatcccg atgacctggc ccgagttcgc cgggatccac ccctatgccc ccgacgccca
     1921 ggccgccggc tatcacgcgc tgatcgatga gttgtcggac tacctgtgtc gcatcaccgg
     1981 ctatgacgcc gtgtcggttc accccaacgc cgggtcccag ggcgagctcg caggtcttct
     2041 cgccatccgc gcgtaccaca ggtcgcgggg cgattcggac cgggagatct gtctcatccc
     2101 ctcctctgct cacggaacca acgcggcatc ggcggtgatg gccggcatga aggtcgtcgt
     2161 ggttgccacc gatgaatcag ggaatgtgga cctggccgat ctggaggcga aggcctcgga
     2221 gcacgccgat cggttggcgg cggcgatgat cacc
//
LOCUS       DATITW010000314         1860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_135471, whole genome shotgun sequence.
ACCESSION   DATITW010000314 DATITW010000000
VERSION     DATITW010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135471"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VLS86_04430"
     CDS             complement(<1..221)
                     /locus_tag="VLS86_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49770.1"
                     /translation="MGQQPNIEATEANRPRSVPQPPAARRWRPIRPGDPTTPGQVPKG
                     GMFGTAAPDGGYALRIISASPLPDDDPDL"
     gene            complement(221..1135)
                     /locus_tag="VLS86_04435"
     CDS             complement(221..1135)
                     /locus_tag="VLS86_04435"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HSO49771.1"
                     /translation="MEPASKIWVDGDFVDWGDATVHFLAHSLQYGTGVFEGIRAYETP
                     EGTAVFRLHEHMVRLVASARAYGLPMKETAEEMDEACLALLRVNELESAYIRPFVFYG
                     ADKIGINPGSAPVHTYMAAFVLGSYMGDDGVQNGIRAMVSSWRRISHSSFIPTAKGSG
                     QYLNSVLAKQEALRKGYDEPIMLNDSGSVSEGSGMNLFTVSNGKVYTPPVSSGILEGI
                     TRNSVMELLKSEGVEVVEIDLARGSLYTEDVVFLTGTAAEVTPMREIDGRPIGSGEPG
                     PITRKAQDLFSDLVAAKLPEYRHWLTFV"
     gene            1215..>1860
                     /locus_tag="VLS86_04440"
     CDS             1215..>1860
                     /locus_tag="VLS86_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene cyclase"
                     /protein_id="HSO49772.1"
                     /translation="MRAEQPARVAIIEWLLEGDPSIRWQVMRDLEPTPEAVWTAEQRR
                     VASEGWGARLLSHQDEDGRWTPHLYGKKWISTTYSMVLLRRLGLPAGDPRALRACALF
                     LDEGLVEDGGIDFSVTRVGSDVCVTAMVLAVFEWFGFDDPSRYRLLDFLLSRQMADGG
                     WNCERQATHGSFHTTISVLEALHENSRAQGPRPADTSVAEARGRDFFLHHRLFRS"
BASE COUNT          315 a          595 c          619 g          331 t
ORIGIN      
        1 ctcagatccg ggtcgtcgtc gggaagcggg gaggcactga tgatccgcag cgcgtaaccg
       61 ccatcggggg ctgcggtgcc gaacatccct cccttgggaa cctgaccggg cgtggtcggg
      121 tccccgggcc ggatggggcg ccatcgtctc gccgctggtg gctgaggcac cgaccggggc
      181 cgattggcct cggtcgcctc gatattgggc tgttggccca tcagacgaag gtgagccagt
      241 ggcgatactc gggaagcttg gccgcaacca gatcggagaa caggtcctgg gccttgcggg
      301 tgatcgggcc cggctccccc gagccaatgg gtcgcccgtc gatctctctc atcggagtca
      361 cctccgccgc ggtcccggtg aggaagacca cgtcttcggt gtacagactg ccccgggcca
      421 ggtcgatctc gacgacttcg accccttcgg acttgagcag ctccatgacc gagtttcggg
      481 tgatcccttc gagaatcccc gaggacaccg gtggcgtgta gaccttcccg ttgctcacgg
      541 tgaagaggtt catcccgctg ccttcggaca ccgacccgct gtcgttgagc atgatcggct
      601 cgtcgtagcc tttccggagc gcctcctgct tggccaggac cgagttcagg tactgacccg
      661 agcccttggc cgtggggatg aagctgctgt gggagatgcg gcgccaactg gagaccatgg
      721 ccctgatccc gttctggacc ccgtcgtcac ccatgtagct gccgaggacg aacgccgcca
      781 tataggtgtg aaccggggcc gacccggggt tgatgccgat cttgtccgcc ccgtagaaga
      841 cgaagggacg gatgtacgcg gactccaact cgttgacgcg cagcaaggcg agacacgcct
      901 catccatctc ctcggcggtc tccttcatcg gcagcccgta ggcgcgggcc gaggcgacca
      961 gtcggaccat gtgctcgtgg agacggaata cggcggttcc ctcgggtgtc tcgtaggcac
     1021 gaatgccctc gaagacaccg gttccatact gaagggaatg tgccaggaag tggacggtcg
     1081 cgtcgcccca atcgacgaag tcgccgtcaa cccatatctt ggatgccggt tccatcggcc
     1141 agaggctact tgctggaatg ggcagggaaa gcggcgagag cgggaaacgg ccaaaggggt
     1201 gtttaatcga aaggatgcga gccgaacagc cggcccgtgt cgcgatcatc gaatggctgt
     1261 tggagggcga cccttcgatt cgctggcagg tgatgcgcga tctggagccg accccggagg
     1321 cggtgtggac cgcggagcag agacgtgtgg ccagcgaagg atggggggcc cggctgttgt
     1381 cgcaccagga cgaggacggg cgctggaccc cgcatctcta cggcaagaag tggatctcga
     1441 ccacctactc catggtcctg ctccgccgac tcggtctgcc tgccggtgac ccaagagccc
     1501 tccgcgcctg cgctctgttc ctcgacgaag gcctggtgga agacggcggg atcgacttct
     1561 ccgtcaccag ggtcgggagc gacgtctgcg tcacggcgat ggtgctcgcc gtcttcgagt
     1621 ggttcggatt cgacgacccg agtcggtacc ggctcctcga cttcctgctc tcccgccaga
     1681 tggccgacgg cgggtggaac tgtgagcgcc aggccaccca cgggtccttt cacaccacca
     1741 tctctgtgct ggaggcgctc catgagaact cccgcgccca gggcccgaga ccggccgaca
     1801 ccagcgtggc cgaagcccgc ggacgtgact tcttcctgca ccacaggctg tttcgatctc
//
LOCUS       DATITW010000315         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_616504, whole genome shotgun sequence.
ACCESSION   DATITW010000315 DATITW010000000
VERSION     DATITW010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616504"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VLS86_04445"
     CDS             complement(<1..964)
                     /locus_tag="VLS86_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSO49773.1"
                     /translation="MSAGEQFDVVVAGAGHNSLVAAAYLSKVGLRCLVLEARPVIGGN
                     TATEELTLPGFHHDTCSTAHNIIQASPTLRYDELKLGEYGLEYIQPDPVVHLPFPDGT
                     SLTMWHDIDRTVAGFERFSKSDAAAYRRMMEDYDRVKGVFAAARYTPYGLGPTLAERM
                     ASHPEGAVWAKRLASSAWDIISREFEHPNTRAFMMWMAFMTVQPPQRSGTGSLAYSLA
                     FGRQRQSWIIPKGGSAALPIALTRMIEAHGGTVIAGRRVESLILENDRCVGVIDETGE
                     EHRAAKAVLSTIHIKHLVDMAPADRWGPEFTSGVEEWRAGIALSP"
     gene            1058..>1530
                     /locus_tag="VLS86_04450"
     CDS             1058..>1530
                     /locus_tag="VLS86_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HSO49774.1"
                     /translation="MTDPMHIDWDVPIEMDDGVVLRADVFRADPDGSYPVILSYGPYA
                     KGLAFQDGYPSAWQRMAEKHPDVAAGSSNLYQSWEVVDPEKWVPHDYACVRVDSRGAG
                     CSPGRVDPFSPRETKDFYDCIEWAGVQPWSNGKVGLNGISYYGINQWHVASLQPP"
BASE COUNT          260 a          510 c          492 g          268 t
ORIGIN      
        1 taggggacag cgcgatcccc gcccgccatt cctcgacacc tgaggtgaat tccgggcccc
       61 atctatcggc cggggccatg tccaccagat gcttgatgtg gatggtcgac agcaccgcct
      121 tggcggctcg gtgctcctcc ccggtctcgt caatgacccc gacacaccgg tcgttctcca
      181 ggatcaggct ttccacgcgc ctcccggcga tcacggttcc cccatgcgcc tcgatcatcc
      241 gggtcagggc aatgggcaag gcagccgaac cgcccttggg gatgatccac gactgcctct
      301 gtcgcccgaa ggcgagcgag taggccagcg acccggtccc cgatcgctgc ggaggctgga
      361 cggtcatgaa ggccatccac atcatgaagg ccctggtgtt cgggtgctcg aactcgcggg
      421 agatgatgtc ccaggcgcta gaggcgagtc ttttcgccca gaccgcgcct tcgggatggc
      481 tcgccatccg ctccgcaagg gtggggccca gaccgtaagg cgtgtaccgg gcagcggcga
      541 agaccccctt cacccggtca tagtcctcca tcatccgccg gtaggcggcg gcgtccgact
      601 tggagaagcg ctcgaagccg gcgaccgtcc gatcgatgtc atgccacatg gtgagcgagg
      661 tcccatcggg gaacgggagg tgaaccaccg ggtcgggttg gatgtactcc agtccgtact
      721 caccgagctt caattcgtcg tatcgcagcg tcgggctcgc ctggataatg ttgtgcgccg
      781 tggagcaggt gtcgtgatga aagcccggga gggtgagctc ctcggtggcg gtgttgcccc
      841 cgatcacggg ccgcgcctcc aggacgaggc aacggagacc cactttcgac agataggccg
      901 cagccaccag gctgttgtga ccggccccgg ccacgaccac gtcgaactgt tctcctgcgc
      961 tcatgcctca tcgactcctt gacgaattcg ccacttcgca aacgattggg cagcgatggc
     1021 tcgggagcag agtagccatt agcctcctgg tccggtcatg accgacccga tgcacatcga
     1081 ctgggacgtg cccatcgaga tggacgacgg tgttgtcctg cgcgccgacg tcttccgagc
     1141 cgatcccgac ggctcgtacc ccgtcatcct ctcctacggg ccctatgcca aggggctcgc
     1201 cttccaggac ggctatccga gcgcgtggca gcgcatggct gagaagcatc cggacgtggc
     1261 cgccggctcc agcaacctct accagagctg ggaggtggtc gacccggaga aatgggtgcc
     1321 gcacgactac gcctgcgtgc gcgtggattc gcgcggcgcc ggatgctcgc cgggccgggt
     1381 cgatccgttc tcgccgcgcg agaccaagga tttctacgac tgcatcgaat gggccggcgt
     1441 gcagccgtgg tcgaacggca aggtcgggct caacggcatc tcgtattacg gcatcaacca
     1501 gtggcacgtg gcgtcgctgc agccgccgca
//
LOCUS       DATITW010000316         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_135635, whole genome shotgun sequence.
ACCESSION   DATITW010000316 DATITW010000000
VERSION     DATITW010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135635"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VLS86_04455"
     CDS             complement(<1..293)
                     /locus_tag="VLS86_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49775.1"
                     /translation="MTALLVSSIGLVVGGPFLAVGTVLVMSLARLRIRRLPSPSIRPV
                     LLVLLVELRAGRSTLAAIQEASAVFPKHRELAMASRLATVKGLTGAVEECHGA"
     gene            complement(290..934)
                     /locus_tag="VLS86_04460"
     CDS             complement(290..934)
                     /locus_tag="VLS86_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49776.1"
                     /translation="MITGALIGWGILAGVDHRRTLVLVLALIAPLPVVALIGIHAWQA
                     RPRVSTRAAVFCEAVAGEMRAGASLRFALERAAASVGASDLEKMSRDGATLSELALAA
                     RTEFEEIGVALGAVIERLFRLGSPAAALFDELAVLSLAQVEVAHEVATAAAPARATAV
                     VLLLVPLVAIGSVTASGRIGDYLSSPAQRIAAVTGLALIILGVGVAGSILSRAR"
     gene            complement(931..1998)
                     /locus_tag="VLS86_04465"
     CDS             complement(931..1998)
                     /locus_tag="VLS86_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HSO49777.1"
                     /translation="MGDAVGVIDRVAGQIASGSVPMRRGDVVVEAERRLRLAAPDRAS
                     QLVGAVVDQVLGLGPLEGLLRDSEVTDVLVNGPQEVWVDRNGELSRTDVSFESSESLL
                     SAVERVIAPLGLRIDRSSPMVDARLPDGSRLHAVTPPASVGHPVVAIRRFTQAVTSLD
                     ALVGLGAATERQVTVLADAVRSRETIIVSGGTGAGKTTLLNLLGSLIPDHERVVTIED
                     AAELRLPGHVVRLEAHPPNSEGAGEITIRSLLRSALRLRPDRIIVGEVRGPEALDLVW
                     ALNTGHRGSLSTVHANSPQEALWRLETLALSAGDTSEKSVRRQLHAAIDHIVQIERIG
                     RQRRIRMVCPVGPDGEIGGDE"
BASE COUNT          399 a          766 c          680 g          303 t
ORIGIN      
        1 atggcaccgt gacattcttc gacggccccg gtgagcccct tgacggtcgc caagcgtgag
       61 gccatcgcca attctcggtg cttcgggaag accgccgagg cttcttggat cgcggccagg
      121 gtcgaccgcc ccgcccgcag ctcgacgagc aggaccaaca ggactggtct gatcgatggg
      181 ctcggcagcc tccggattcg cagcctggcc agggacatca cgagcactgt gccgaccgcc
      241 aggaagggcc ctcccaccac caggccaatc gagctcacca ggagggcggt caacgtgccc
      301 tactcagtat cgagcccgcc acccccaccc ccaagatgat gagggccaac ccggtcaccg
      361 ccgcaatccg ctgcgcaggg ctcgataggt aatcgccaat ccgaccgctg gccgtgacgg
      421 agccaatggc gaccagaggg accagcaaca gcacgacggc cgtggcccgg gcaggcgccg
      481 ccgcggtggc gacttcatga gcaacctcca cctgagccaa cgacaggacc gccagctcat
      541 cgaagagggc ggcagccggc gaaccgagcc ggaagaggcg ctcgatgacc gcacccaacg
      601 cgacaccgat ctcctcgaac tccgtgcgtg cggcgagggc cagttcggac agggtcgccc
      661 cgtcccggct catcttctcg agatcggatg cacccaccga ggctgcggct cgttccaagg
      721 cgaatcgaag cgaagccccg gctcgcatct cacccgcgac cgcctcgcag aacaccgccg
      781 ctcgggtcga aacccgaggc cgggcttgcc aggcatggat cccaatgagt gcgacgaccg
      841 ggaggggcgc gatcagagct aggaccagga cgagggtccg gcggtggtcg acgccggcga
      901 ggattcccca accgatgagg gcgccagtga tcattcatcg cccccgatct caccgtcggg
      961 gcctacaggg cacaccatcc ggatccgacg ttgccgcccg atccgctcga tctggacgat
     1021 gtgatcgatc gcggcgtgga gttggcggcg caccgatttc tctgaggtgt ccccagcgga
     1081 cagagccagg gtctccagtc gccaaagggc ttcttggggc gagttggcgt gaaccgtcga
     1141 gagtgagccc cggtgaccgg tgttcaacgc ccagaccagg tcgagcgcct ccggtccccg
     1201 gacctccccc acgattatcc ggtcgggtcg caggcgaagg gcggacctca gaagagaccg
     1261 aatcgtgatc tcgcccgcgc cttcggagtt cggtgggtgc gcctccagac ggaccacatg
     1321 acctggaagt ctcaactcag cggcatcctc gatggtcacc acccgctcgt ggtcgggaat
     1381 gagacttccg agaaggttga gcagggttgt cttcccggcg ccggttccgc ccgaaacgat
     1441 gatggtctcc cgcgatcgca ccgcatcggc gagcaccgtc acctgccgtt cggttgcggc
     1501 tcccaggccg acgagcgcat ccagcgaggt gacggcctgg gtgaagcgcc tgatcgccac
     1561 aacaggatgc cctaccgatg ccggtggcgt cacggcgtga agtcgactcc catcgggaag
     1621 ccgggcatcg accatgggcg acgaacgatc gatccgcagc ccaagcggtg cgatcacgcg
     1681 ctccacggct gaaaggagcg actcgctgct ctcgaagctg acgtcggtcc gactcagttc
     1741 accgttgcga tcgacccaga cttcctgtgg gccgttgacg agcacgtcgg tcacctctga
     1801 gtcgcgcaat agcccctcga gcggcccaag accaaggacc tgatccacga ccgcgccaac
     1861 gagttgactt gcccgatccg gtgccgccaa gcgaagccgg cgctccgcct cgacgactac
     1921 gtcaccacgt cgcatcggga ccgagccgga agcgatctgg ccggccacgc gatcgatcac
     1981 gcccacggca tcgcccatac cctctcccat acccggagcc accttccgcg tgccggcagc
     2041 cgaccggcga gaaggttgcg cgcaccccgt gggcccagtc tgggcaagac cggtcccggc
     2101 cccggcgggc gcactcccgc aaccaccgtt tgccacaccg cggggagg
//
LOCUS       DATITW010000317         1831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_96067, whole genome shotgun sequence.
ACCESSION   DATITW010000317 DATITW010000000
VERSION     DATITW010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96067"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1831
                     /gene="aceE"
                     /locus_tag="VLS86_04470"
     CDS             <1..>1831
                     /gene="aceE"
                     /locus_tag="VLS86_04470"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018816622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HSO49778.1"
                     /translation="PDRARFLMAKLLERAHETSIGVPGSITTPYINTIAAEDQPEFPG
                     DETIERRIRRFIRWNAAVMVVKANHREKGIGGHLSTFASSATLYEIGFNHFFKGKGAP
                     GGGDHVYMQGHAAPGIYARAFLEGRFEEKHLDNFRFEIGGDGLSSYPHPRLMPDFWEY
                     PTVSMGIGPINSIYHARFNKYLHNRGLDDTSQSRVWCFIGDGETDEPETLGAISLATR
                     EGLDTLTWILTCNLQRLDGPVRGNSQIISELEAVFRGAGWNVIKVIWGTGWDRLLDND
                     KEGALISVMGSTVDGEYQRYKTEDGAYVREHFFGKDPRTKAMVADMTDDEIWALRRGG
                     LDTRKVYAAYKVATELTNGKPPVILAKTIKGWTLGPDVEGRNATHQIKTLTNQQLMTL
                     RETLHLDQEIPAEALEGESEPPYFRPGPGTPEYEYLHQRRQELGGLLPMRSVPETQLE
                     LPVPEAYAEIHAGSGKVEASTTTAFTRLIRNLSRVEGFGDRIVPIVPDEARTFGMDSL
                     FRELKIYASRGQLYEAVDASMLLSYTESQDGQILEEGITEAGSVASWIAAATSYATRG
                     LPMVPFFIFYSMFGFQRVGDLNWSAPDSRARGFLLGATAGRTTL"
BASE COUNT          351 a          596 c          571 g          313 t
ORIGIN      
        1 gacccgaccg tgcccgtttc ctcatggcca agctgctgga gcgggcccat gagaccagca
       61 tcggcgtgcc cgggtcgatc accaccccgt acatcaacac catcgcggcc gaggaccaac
      121 ccgagttccc cggtgacgag accatcgagc gcaggatccg caggtttatt cgctggaacg
      181 cggcggtcat ggtcgtcaag gcgaatcatc gggagaaggg catcggtggc catctctcca
      241 cgttcgcctc gtcggcgact ctctacgaga tcgggttcaa ccacttcttc aagggcaagg
      301 gcgctcccgg tggtggcgac catgtctaca tgcaggggca tgccgccccc gggatttatg
      361 cgagggcatt cctcgagggc cgattcgagg agaagcacct ggacaacttc cggttcgaga
      421 tcggtggcga cgggctctcc tcctaccccc atccccgtct catgcccgac ttctgggagt
      481 acccgaccgt gtccatgggc atcgggccga tcaactcgat ctatcacgcc agattcaaca
      541 aatacctgca taaccgcggt ctcgacgaca cttcccaatc ccgggtctgg tgcttcatcg
      601 gcgacgggga gaccgacgag ccagagacct tgggcgctat ctcgctcgcg acccgtgaag
      661 gcctcgacac cctgacctgg atcctcacct gcaacctgca gcgactcgac ggcccggtcc
      721 ggggcaacag ccagataatc agcgagctgg aagcggtatt ccgcggcgcg ggctggaacg
      781 tgatcaaggt gatctggggc accggttggg accggctgtt ggacaacgac aaggaaggcg
      841 ccctgatctc ggtcatggga agcaccgtcg acggtgagta ccagaggtac aagaccgagg
      901 acggggccta cgtgagggaa cacttcttcg gcaaggaccc ccgcaccaag gcgatggtgg
      961 ccgacatgac cgacgacgag atctgggccc tgcggagggg aggcctggac actcgcaagg
     1021 tatacgcggc atacaaagtg gccaccgagc tcaccaacgg gaaaccgccg gtgatcctgg
     1081 ccaagaccat caagggctgg acgctgggac ccgatgtgga gggccggaac gcgacccacc
     1141 agatcaagac cctcaccaac caacagttga tgaccctgcg ggagaccctt catctcgatc
     1201 aggagatccc ggccgaggct ctggagggag aaagcgagcc gccctacttc cgcccggggc
     1261 cggggactcc tgaatacgag tacctgcacc agcgtcgtca ggagttgggc gggctgttgc
     1321 cgatgcggtc ggtgcccgag acccagctgg aattgccggt ccccgaggcc tacgccgaga
     1381 tccacgccgg atcggggaag gtcgaggcct cgacgaccac ggctttcact cgcctcatcc
     1441 gcaatctgtc ccgggtggag ggctttgggg accggatcgt gcccatagtc ccggacgagg
     1501 cccgcacctt tggaatggac tcgttgttcc gcgagctcaa gatctacgcc tcgcgcggtc
     1561 agctctacga agcggtcgac gcctcgatgc tcctctccta taccgagtcc caggacggtc
     1621 agatcctcga agagggaatc accgaagcgg ggtcggttgc ctcctggatc gcggctgcca
     1681 cctcatatgc gacccgcggc ctgccgatgg tccccttctt catcttctac tcgatgttcg
     1741 gctttcagcg ggttggcgac ctgaattggt ccgctccaga ctcgagggcc cgtgggttcc
     1801 tccttggtgc caccgcgggg cggaccacgc t
//
LOCUS       DATITW010000318         1547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_536776, whole genome shotgun sequence.
ACCESSION   DATITW010000318 DATITW010000000
VERSION     DATITW010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536776"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VLS86_04475"
     CDS             complement(<1..463)
                     /locus_tag="VLS86_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HSO49779.1"
                     /translation="MRFVARGDEIVLKGDEVTVEKARTVLAELLILVQEGHPLDSGRV
                     DEVVKMVRDEVPSPSGILTDGIPVGRGRMVRPKTHGQQKYIGAIRENTLTFGLGPAGT
                     GKTYLAMAAAVDGLVSGSVRRIILTRPAVEAGERLGFLPGDLAAKVDPYLRP"
     gene            complement(649..1116)
                     /locus_tag="VLS86_04480"
     CDS             complement(649..1116)
                     /locus_tag="VLS86_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSO49780.1"
                     /translation="MGVDDLLVSAQGSLGEQSLVDKFSASLGVRLRAARRQRGWSLGE
                     VEAFTDGEFKASVVGAYERGDRAISVQRLTRLAEVYGVGPTELFPGSKPTEGPVIDID
                     AVAAGRDHDMVDRYLAAIQFMRREGTPGEVRDSDKAVISSLLETSIDTVSESD"
     gene            complement(1106..>1547)
                     /locus_tag="VLS86_04485"
     CDS             complement(1106..>1547)
                     /locus_tag="VLS86_04485"
                     /inference="COORDINATES: protein motif:HMM:NF016347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HSO49781.1"
                     /translation="NPLTLAVAPPASRDRCRFLVEKLSELGVARLVWLATSHGEGRSP
                     HAGKTDLWAASALEQSRGGWLMEVGTDLVSWDDLDRPLVVAVPGGGSDVFEVRTVAVG
                     PEGGLDPAEIPADAASIDLGSTILRVETAAVIAASRFSRPWRGG"
BASE COUNT          277 a          528 c          487 g          255 t
ORIGIN      
        1 gcgggcggag atacgggtcg actttggcgg cgaggtcccc ggggaggaac ccgagccgct
       61 ccccggcctc caccgcgggt cgggtcagga tgatcctgcg caccgatccg ctgaccaacc
      121 cgtcaaccgc cgccgccatc gccagatagg tcttcccggt cccggcggga ccgagaccga
      181 aggtgagagt gttctcccgg atcgcaccga tgtacttctg ctgaccgtgg gtcttggggc
      241 ggaccatcct cccccggcct accgggatcc cgtcggtgag gatccccgat gggctgggaa
      301 cctcgtcgcg gaccatcttc acgacctcgt cgacccggcc gctgtcgagt ggatggccct
      361 cctggaccag gatcagcaac tcggcgagga cggttcgcgc cttctccact gtgacttcgt
      421 cgcccttgag cacgatctcg tctccgcgcg cgacaaagcg cacctccggg aaatggtcct
      481 cgacttgacg gagatgacgg tcggaatccc caagtaggtc cagcaacaag tgatggctgg
      541 ggacgcgtaa atgcgattcg atgtggtcag gagacacgga aaacgacgcc ctgttgtggc
      601 gccacggtaa ttctatccag ccaggggaat tgggctatta cttcgatctc agtcggactc
      661 ggagacggtg tcgatcgaag tctcgaggag ggaggagatc acagccttgt ccgaatcacg
      721 gacttccccg ggagtgccct cgcgccgcat gaactggatg gctgcgaggt agcggtccac
      781 catgtcgtgg tcccggccgg ccgccacggc gtcgatgtcg atgaccggtc cttcggttgg
      841 cttggaacca gggaacaatt cggtcggccc cacgccatag acctctgcga gccgtgtcag
      901 acgttgcact gagatggcgc ggtccccgcg ttcgtaggcg cccacgaccg acgccttgaa
      961 ctcgccgtcg gtgaaagcct ccacctcacc caatgaccag cctcgctgac ggcgagccgc
     1021 ccgaaggcgg acgccaagag aggcgctgaa tttgtcgacc aaagattgct ctcccagtga
     1081 gccctgcgca gatactagaa ggtcgtcaac ccccacgcca gggtctgctg aaacggctcg
     1141 cagcgatcac cgcggcggtc tccaccctga ggatcgtcga tccgaggtcg atggaagccg
     1201 catcggccgg gatctcagcc ggatcgagcc ctccctcagg cccgacggcg accgttctca
     1261 cctcgaatac gtcgctccca ccgccgggta cggccaccac cagcggccgg tccagatcgt
     1321 cccaggacac caggtcggtc ccgacttcca tgagccagcc accccgcgac tgctccaggg
     1381 ccgacgccgc ccagagatcg gtcttgccgg cgtgggggct ccggccctcg ccatggctgg
     1441 tagccagcca taccaggcgg gctacgccca gttcggacag cttctccacc agaaaccggc
     1501 aacgatcacg gctggccggt ggggccacgg ccagagtgag cgggttc
//
LOCUS       DATITW010000319         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_135858, whole genome shotgun sequence.
ACCESSION   DATITW010000319 DATITW010000000
VERSION     DATITW010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135858"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VLS86_04490"
     CDS             complement(<1..354)
                     /locus_tag="VLS86_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the reversible isomerization of glucose
                     6-phosphate to fructose 6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HSO49782.1"
                     /translation="MSDMLAMIRTLGEQLRWARDLELPAIAPSSELLIGGMGGSGIAG
                     DFAAALSVKTPGRVAVHKGYAPLPGWASRVGPTVIAISYSGNTEETLSLVTAAGEAGL
                     PVVTITTGGQLAEMSR"
     gene            complement(347..526)
                     /locus_tag="VLS86_04495"
     CDS             complement(347..526)
                     /locus_tag="VLS86_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007794429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HSO49783.1"
                     /translation="MSLIHPELAAVLVCPVDKGELTEDVEASRLVCKTCSREYPVRDG
                     IPIMLIDEADGDSDE"
     gene            complement(523..783)
                     /locus_tag="VLS86_04500"
     CDS             complement(523..783)
                     /locus_tag="VLS86_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49784.1"
                     /translation="MELYAASGELNFEVADPAASIALIESAFSLAEIDRLDGLSVDLG
                     DSWFNVRASNTEPLLRLNVEARDPSTVADTVRRIESILQEPQ"
     gene            complement(796..966)
                     /gene="manB"
                     /locus_tag="VLS86_04505"
                     /pseudo
     CDS             complement(796..966)
                     /gene="manB"
                     /locus_tag="VLS86_04505"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; incomplete;
                     partial in the middle of a contig; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
     gene            complement(985..1866)
                     /locus_tag="VLS86_04510"
     CDS             complement(985..1866)
                     /locus_tag="VLS86_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49785.1"
                     /translation="MIQDAVFKAYDIRGRADNGEIEPKLFRLIGQAFVSLFPAEVVAV
                     GRDVRVSSPALFEGLVDGITAAGSDVIDLGEVPTDLVYFYSGKHSVPGAIITASHNPS
                     EYNGLKLCRAGAAPVGADTGLAEIKDLVLGGGSDTGATRGSVSRVDAVDDYVDHLLSI
                     VDAASIGPLRVAADGGNGMAGITVTRVFDRLAADLDGLYLDPDGTFPNHPADPIQLEN
                     LSDLIDKVTKGGHDLGVAFDGDADRAFFIDDQASPVSGSTVTALIARWMLHRHPGASI
                     IHNLITSRAVAEVVREA"
     gene            1926..2666
                     /locus_tag="VLS86_04515"
     CDS             1926..2666
                     /locus_tag="VLS86_04515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSO49786.1"
                     /translation="MRPIFSIVVPVYNEATFIPRALPALIEEIEDLAETYRILIVENG
                     STDGSAEAAREAGAGFPVTVLSLPAPDYGAAMRHGFLEADGEWVVNFDIDYFSAGFLR
                     HVLEMQDVDLVIGSKRAPESEDRRPMMRRMATTVFNLLLSTVLDSRVSDTHGMKAFRR
                     GLIEEIAPAVVSRQDLFDTELVVRAERAGYRIREVPVVVEEMRVARSSLVKRVPRTLR
                     GLWRIRKVLADETRRPATPAPAPDNDSD"
BASE COUNT          479 a          901 c          831 g          469 t
ORIGIN      
        1 gcgggacatc tcggctagct ggccgccggt cgtgatcgtg accaccggca gaccggcctc
       61 accggcggca gtgaccaggc tcagtgtctc ctcggtgttg cccgagtagg agatggcgat
      121 caccgtcggg cccacccgag acgcccaacc gggcaggggg gcgtatccct tgtgcacggc
      181 cacccggcca ggagtcttga ccgagagcgc agctgcgaag tctccggcaa tccccgagcc
      241 tcccatgccc ccgatcagga gctctgagct gggtgcgatc gcaggcagtt ccaggtctct
      301 cgcccagcga agctgctcac ccagcgttcg gatcatcgcc aacatgtcac tcatcgctat
      361 ccccgtcggc ttcatcgatc agcatgatcg gtatcccgtc gcgcaccgga tactcgcggc
      421 tgcaggtttt gcagaccaat cgcgacgcct cgacgtcctc ggtcagctcg cccttgtcca
      481 cggggcagac gagcacggct gccagctcgg gatggatcag gctcattgcg gctcctggag
      541 gatgctctcg atgcggcgga cggtgtcggc gaccgtggat ggatcgcggg cctccacgtt
      601 gagccggagc agcggctcgg tgttggaagc acgcacattg aaccaggagt caccgagatc
      661 cacggacaga ccgtcgagtc ggtcgatctc ggccagggag aatgccgatt cgatcagggc
      721 gatcgaggcc gcgggatccg ctacctcgaa attgagctcg cctgaggcgg cgtacagctc
      781 cacgtcccgt ctcagagccg acaacgggcg accgtcctcc gatatcaccc tcatcaacac
      841 caacatcgcc agcattcccg agtccgcccg ataattgtcg aggaagtagt agtgaccgct
      901 gtgctcgcca ccgaaaacag ctccggattc agccatcacc tgcttgatgt agctgtgacc
      961 gacccgggtc ctgaccggga ctcctcacgc ttctctgacc acctcggcga ctgcccgcga
     1021 ggtgatgagg ttgtggatga tcgacgcccc cgggtgccgg tgcagcatcc agcgggcgat
     1081 gagcgccgtg acggtgctgc cgctgaccgg agacgcctgg tcgtcgatga agaacgcccg
     1141 atccgcatcg ccgtcgaaag ccactcccag gtcgtgaccg cccttcgtca ccttgtcgat
     1201 gaggtcgctg agattctcca gttggatcgg gtcggcggga tgattgggga aggtgccgtc
     1261 aggatcgaga tagagcccgt cgaggtccgc cgcaagccgg tcgaagaccc tggtaaccgt
     1321 gatgccggcc atgccattgc ccccatcggc cgccacccgg agcgggccga tggatgctgc
     1381 gtcgactatg gacaggaggt ggtcgacata gtcatccact gcgtcgaccc tgctcaccga
     1441 gcctctcgtc gcccccgtgt ctgacccacc acccagaacc aggtccttga tctccgccag
     1501 cccggtgtcc gctcccaccg gcgccgcccc ggcccggcac agcttcaggc cgttgtactc
     1561 ggacggattg tgagacgcgg tgatgatcgc cccggggacc gagtgctttc ccgagtagaa
     1621 gtagacgaga tcggtcggta cctcaccgag atcgatcaca tcagagccgg ctgccgtgat
     1681 cccgtcgacc agtccttcga aaagagccgg cgacgacacc cgaacgtccc tgcccactgc
     1741 caccacctct gccgggaaca gggaaacgaa ggcttggccg atgagacgaa acagcttggg
     1801 ctcgatctcg ccgttgtcgg cgcgaccacg gatgtcgtat gccttgaata cggcgtcttg
     1861 gatcatcgga cgccgagtct aggggtgggt ctatgtcttc ctgattccta cactccacca
     1921 ctccgatgcg ccccatcttc tccatcgtcg tccctgtcta caacgaggcg accttcatcc
     1981 ccagggcgct tccggcgttg atcgaggaga tcgaagacct cgccgagacc tatcgaatcc
     2041 tgatcgtcga gaatggctcg acggacgggt cggcggaggc ggcccgggag gccggcgccg
     2101 gctttccggt caccgtgctc tccctaccgg cacccgacta cggggcggcc atgaggcatg
     2161 ggtttctcga ggccgacggc gaatgggtgg tgaacttcga catcgactac ttctcggcag
     2221 ggttccttcg tcacgtcctc gagatgcagg acgtcgacct ggtcatcggc tcgaagcgag
     2281 ccccggagtc agaagaccgc cgccccatga tgcgccgcat ggcgacgact gtgttcaatc
     2341 tgctgctcag caccgtcctc gactcccggg tgtcggacac ccacgggatg aaggcattcc
     2401 gacggggcct gatcgaagag atcgcgcccg ccgtcgtcag tcgtcaggat ctgttcgaca
     2461 ccgagctggt ggtgcgcgca gaacgcgccg gctacaggat tcgagaggtc ccggtggtcg
     2521 tcgaggagat gcgagtcgcc cgctcgtcgc tagtcaagag agtgccgcgc accctccgcg
     2581 gcctgtggag gatccgaaag gtcctcgccg atgagacccg gcgcccggcc actccggcgc
     2641 cggctccaga caacgactcc gactaacccg acaattccga
//
LOCUS       DATITW010000320         1575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_336493, whole genome shotgun sequence.
ACCESSION   DATITW010000320 DATITW010000000
VERSION     DATITW010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336493"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VLS86_04520"
     CDS             <1..1353
                     /locus_tag="VLS86_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HSO49787.1"
                     /translation="MLTSPANLFLSAHPRPKGAMRAMPNLLEADDIDSIGASLASDLT
                     WKQIFDTDACTICGRCTSVCPANITGKPLDPREIGLKSGEVAAADAGVSPPLSSVMML
                     EGEHSLFERVRPEEVFACTTCRACDEICPVNIEILDRILDMRGYLTLMESEFPSELGK
                     AFVAMENQSNPWGMSQQNRASWTAELDFEVPVFGENGKTTADYLWFVGCAGSFDDRNT
                     AVSVALARLINKAGIDFAILGRAEVCNGDPERRAGNEFVFQQLALQNIASFGEVGVTR
                     VITQCAHCFNTIANEYPQFGGNYEVVHHSQLLAELLSQGRLTVPTKENGQPKRIAYHD
                     PCYLGRHNDVYLAPRNVIGASGDIEVVEMPRHGTRALCCGAGGARFWMEEQTGKKVNI
                     ERAEEALATEADEIAVACPFCLVMLDDGVKELGRDDVKVRDLAMILAEGVGPAGAGAG
                     "
BASE COUNT          292 a          481 c          528 g          274 t
ORIGIN      
        1 atgctcacct cgccggcgaa tctgtttctc tccgcacatc cgaggcccaa gggcgcgatg
       61 cgggccatgc ccaacctgct cgaggccgat gacatcgaca gcatcggcgc ctcattggcc
      121 tcggatctca cctggaagca gatcttcgac accgatgcct gcaccatctg tgggagatgc
      181 acctcggtgt gcccggccaa catcaccggc aagcccctcg atccgagaga gatcggcctc
      241 aagagcggcg aggttgctgc ggcggacgcc ggagtcagcc cgccgctgtc gtcggtcatg
      301 atgttggagg gtgagcactc actcttcgag cgggtgcggc ccgaggaggt attcgcctgc
      361 accacctgtc gggcctgtga cgagatctgc ccggtgaaca tcgagatcct cgatcgcatc
      421 ctcgacatgc gcggctatct caccttgatg gaatcggagt tcccgagcga gttgggcaag
      481 gcgttcgtgg ccatggagaa ccagtccaat ccctggggca tgagccagca gaatcgagcc
      541 tcatggacgg ccgagctcga cttcgaggtc ccggtgttcg gtgagaacgg gaagaccacc
      601 gccgactatc tctggttcgt gggttgtgcc gggtccttcg acgacaggaa caccgcggtc
      661 tcggtggctc tcgccaggct gatcaacaag gcagggatcg actttgccat cctcggcaga
      721 gccgaggtct gcaacgggga tccggagcgg cgcgccggca acgagttcgt cttccagcag
      781 ttggcactgc agaacatcgc ctccttcggc gaggtcgggg tcaccagggt catcacccag
      841 tgcgcccatt gcttcaacac cattgccaac gagtatccgc agttcggcgg caactacgag
      901 gtggtgcatc acagtcagct cctggctgag ttgctgagtc aggggcggct cacggtcccg
      961 accaaggaga acgggcagcc caagagaatc gcgtaccacg acccctgcta tctgggcagg
     1021 cacaacgatg tgtatctggc gccacggaac gtgatcggcg cctcgggcga catcgaagtc
     1081 gtggagatgc cacgtcatgg cacccgtgcg ctgtgttgtg gcgccggcgg agctcggttc
     1141 tggatggagg agcagaccgg gaagaaggtc aacatcgaga gagcggagga ggccctagcc
     1201 accgaggcgg acgagatcgc agtggcctgc ccattctgct tggtgatgct cgacgacggg
     1261 gtgaaggagc tggggcgcga cgatgtgaag gtgagggacc tggcgatgat ccttgccgaa
     1321 ggagtggggc ccgcaggggc cggagccggg tgattcgacg attagcgcct ctacttgggc
     1381 tggtcgtggc tatcggaggg tgcacacctg accttggcgg cccgtcggga accgacccgg
     1441 tgtcgaccac gaccacggcg cccaccacca cctcgaccac aactgtcacc ggggccgcgg
     1501 ttgagacctt tcgggcttgt ctcggtgagc acggtgtcgt gatcgaggag gtgccctacg
     1561 acgccaccgg gcgac
//
LOCUS       DATITW010000321         1597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_16277, whole genome shotgun sequence.
ACCESSION   DATITW010000321 DATITW010000000
VERSION     DATITW010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16277"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /gene="dprA"
                     /locus_tag="VLS86_04525"
     CDS             <1..833
                     /gene="dprA"
                     /locus_tag="VLS86_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HSO49788.1"
                     /translation="GGMTEPRLVGPADEGYPAGLFDLESPPTLYVLGATTTAPAVAVV
                     GTRKCTRYGLALGEAFGHALADAGWTTVSGLARGIDAAAHRGTLGAEGEAVAVLGSGI
                     DVIYPAEHRGLYHQILDGNGAIMSEYPPGTPPDRWRFPARNRLIAAIASAVVVVEAGH
                     QGGALITARLAAELGRPVFAVPGDVDRPASKGCNLLIRDGAFPVLGVADLIAELELVL
                     GKPPRTEMPATSGIPATGVVIDDLPRVWGCTPAEALARLARMEIAGEIRRSGEVIIPI
                     "
     gene            871..>1597
                     /locus_tag="VLS86_04530"
     CDS             871..>1597
                     /locus_tag="VLS86_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HSO49789.1"
                     /translation="MHLPDMPAWALGPVDEYLIRLREQRDLSPNTVDAYRRDLSQFFD
                     YCDRNGVKQIAAVQRINARRYLAFLDTRGYSRRSATRKASAVRSFYNDAGRRGVATVN
                     PFEGVARPRLDRPLPHALPTRTVIHAIESIDTATPEGLRDRAVLDTLYSTGFRISEMA
                     SLTVSDIGGDMITVVGKGRKTRRVPVGRPAQESLRRYLADGRPRLASAEAGNWLWVGS
                     RGGRLDTRGIRRVVKDRMATFPHA"
BASE COUNT          244 a          513 c          560 g          280 t
ORIGIN      
        1 atggcgggat gactgaacct cgtctcgtcg gccccgctga cgaggggtat ccggccgggc
       61 tgttcgatct ggagagcccg ccgaccctat atgtgctggg ggcgaccacc actgctccag
      121 cggttgcggt ggtcgggacc aggaagtgca cccggtatgg gttggcactt ggcgaagcgt
      181 tcggtcacgc attggccgac gcgggttgga ccacggtcag cggtctggct cgcggcatcg
      241 acgccgccgc tcaccgcggg acactgggtg ctgaaggcga agcggtggcg gtgctcggct
      301 ccggaatcga tgtgatctat ccagctgagc atcgcgggct ctatcaccag atcctcgacg
      361 ggaatggggc gatcatgtcc gagtacccgc cgggcactcc tcccgaccgt tggcggttcc
      421 cggcgcgaaa ccggttgatc gcggcgatcg cctcggccgt ggtggtggtc gaagccgggc
      481 accaaggggg cgctctgatc accgcccgtc tcgccgcgga gctgggtcgg ccggtctttg
      541 ccgtccctgg cgatgttgat cggccggcgt cgaagggctg caacctgctg attcgcgacg
      601 gggcgttccc ggttctcggg gtggcggact tgattgccga gttggagttg gtcctgggaa
      661 agccgccccg aaccgagatg ccggcgacaa gcgggatccc ggcgacggga gtcgtcatcg
      721 atgatctgcc ccgagtgtgg ggctgcactc cggccgaggc cctggcccgg ttggcgagga
      781 tggagatcgc gggcgagatc cgccgctccg gagaggtgat catcccgatc tgaactggtt
      841 ctcgcgaaat ctcgctttag gatcagagca gtgcatctcc ccgacatgcc ggcgtgggcc
      901 ttgggcccgg tcgacgagta cctgatccgt ctccgcgagc agcgggacct gtcgccgaac
      961 acggttgacg cttatagacg cgatctcagc cagttcttcg actactgcga tcgcaatggg
     1021 gtgaagcaga tcgccgccgt gcaacggatc aacgcccgcc gttatctggc ctttctcgac
     1081 acccgcggct actcgaggcg ctcggccact cgcaaggcct ccgcggttcg ttccttctac
     1141 aacgacgcgg gtcgaagagg ggtggcgacg gtgaacccct tcgagggcgt ggcccgcccc
     1201 cggctcgacc gtcccttgcc tcacgccctc cctacccgga cggtgatcca cgccatcgag
     1261 agcatcgaca ccgctactcc cgagggactg cgcgaccgag cggtgctcga caccttgtac
     1321 tccacagggt tccgcatctc cgagatggct tcgctcaccg tgagcgacat cggtggcgac
     1381 atgatcacgg tggtcggcaa ggggaggaag acgagacggg tgccggtggg tcgcccggcg
     1441 caggagtcgc ttcgccgcta tctggcagat ggccgccccc ggctggcgtc cgccgaggcc
     1501 gggaactggc tgtgggtggg ctcgcgggga ggccggctcg acaccagagg gattcgtaga
     1561 gtggtcaagg accggatggc gaccttcccc cacgccc
//
LOCUS       DATITW010000322         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_336497, whole genome shotgun sequence.
ACCESSION   DATITW010000322 DATITW010000000
VERSION     DATITW010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336497"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VLS86_04535"
     CDS             <1..644
                     /locus_tag="VLS86_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSO49790.1"
                     /translation="RVREVDGEVEIEVGGRVHRVGALIVAAGAWTNRVLAHFGVGFPL
                     EVTLEQVVYLRSGDPDSFHPSRFPVWIWMDEPSFYGFPIFGEPAVKIAWDRCEIVTDP
                     DARSFEPRSDVIDGVRGFAEERLPGAAGEVHLAKTCLYTLTPDRDFVVDRVPGTANVY
                     TAVGAGHAFKFASLLGQILSDLALDGSTPHDIDVFAADRPILQERSPARAYMV"
     gene            705..>2226
                     /locus_tag="VLS86_04540"
     CDS             705..>2226
                     /locus_tag="VLS86_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSO49791.1"
                     /translation="MKRERLDAAGNEIDRLGALNELSAALYEYDSAEAVAVAEEAVSL
                     ADSLGNGLARAWALHHRGWALSSLGRLDEALDDQLSARAQFEIEGDMLGVANTLMAIG
                     DLYGDVGDNSTALEYLERARAPLKLADDALGMGLLMNLTGIALSHAGRHAEALDLFER
                     AEIVYGDLGDVLRVGTARINQGYELLLLAERADEPEKQELIARVEHLAFEIVERGEIL
                     GEDGRHTIAYGKSLIAQVQAASGRVSEALAFSEGAEADASAGGLDQLAIETALDRAGW
                     LIEVGRIAEASELLDQVTEKSHKLDMRRAIARAAEMRVDVLEAAGDYAGALASFREFH
                     QLDGFLHSDQAERRARLIATRYQVERARQETELAQVRVSELEALDHDKRDFLASISHE
                     LRTPLAAVLGFATELSDSWDHFEPEEARSLVRLIATQSADISSSVDDLLTVTRLEAGT
                     MSIYPTAIDVAQHLADMVETSGRESGRVIDWKGNALVWADPTRLRQIVRNLITNAIR"
BASE COUNT          384 a          726 c          732 g          384 t
ORIGIN      
        1 cccgtgttcg cgaggtggac ggcgaggtgg agatcgaagt cggtggccgc gttcaccggg
       61 ttggcgccct gatcgtggcc gcgggggcgt ggaccaatcg cgtcctcgcc catttcggcg
      121 tgggcttccc actcgaggtg acgctggaac aggtcgtcta tctgcgatca ggcgaccctg
      181 attccttcca cccctcccgg ttcccggtgt ggatctggat ggacgagccg tcgttctatg
      241 gattccccat cttcggtgag cccgcggtga agatcgcatg ggatcgctgc gagatcgtca
      301 ccgaccccga cgcccgcagc ttcgagccca ggtcggacgt aatcgacgga gtcagaggct
      361 tcgccgagga gcgactcccg ggagcagcgg gtgaggtcca cctggccaag acgtgtctct
      421 acaccctgac tcccgaccgc gacttcgtgg tcgaccgggt cccgggcacc gccaacgtat
      481 acaccgcggt cggtgccggc cacgctttca agttcgcatc gctgctcggc caaatcctca
      541 gtgacctcgc cctcgacggt tcgacaccgc acgacatcga cgtcttcgcc gccgaccggc
      601 ccatcctcca ggaaaggagc ccggcgcggg cctacatggt ctgacgccgg tatcaagtgc
      661 tcgtgtttca aggccgattt cttccagaga tggacactct ctccatgaag cgagagcggc
      721 tcgacgctgc ggggaacgag atcgaccggc ttggcgcgct caacgagctg agtgcggctc
      781 tctacgagta cgactcggcc gaggcggtcg ctgtggccga ggaagcagtc tccctcgccg
      841 attcccttgg caacggactg gcccgagcct gggcgctgca tcatcggggt tgggccttga
      901 gctcactggg ccgcctcgat gaggcgctcg atgatcagct ctccgctcgg gcccagttcg
      961 agatcgaggg cgacatgctt ggcgtggcga acaccctgat ggcgatcggc gatctctatg
     1021 gggacgtggg tgacaattcg accgccctcg agtatctgga gcgagcgcgg gctcccctca
     1081 agctcgctga cgacgctctc gggatgggtc tgctgatgaa cctgaccggg atcgccctga
     1141 gccacgccgg gcgacatgcc gaggcgctgg acctcttcga gcgcgccgag atcgtctatg
     1201 gcgatctcgg agatgtgctc cgggtgggca ccgctcgaat caaccaggga tacgaactgc
     1261 tcctcctcgc ggagagagca gacgagcccg agaagcaaga gctgatcgcc agggtcgagc
     1321 atctggcctt cgagatcgtc gagcggggag agatcctcgg tgaagatggg cgtcacacca
     1381 tcgcttacgg caagtccctg atcgcgcagg tgcaggccgc ctcaggtcgt gtcagcgagg
     1441 cgcttgcctt ctccgaaggc gcagaggccg acgcctcggc ggggggtctc gatcaactgg
     1501 cgatcgaaac ggctctcgac cgggcggggt ggctgatcga agtgggacgg atcgcggagg
     1561 cttccgagtt gctcgatcag gtgacggaga agagccataa gctcgacatg cgtcgcgcca
     1621 tagccagggc tgccgagatg cgggtggacg tgctcgaagc cgccggcgac tacgcgggcg
     1681 cacttgcctc tttccgtgag tttcaccaac tcgacggctt tctccacagc gaccaggccg
     1741 agcgccgggc gcgtctcatc gcgacccgct accaggtcga gcgtgcacgc caggagaccg
     1801 aactagccca ggtgcgggtg tcggagctgg aggccctcga ccacgacaag cgcgactttc
     1861 tcgccagcat cagccacgag ctgaggacgc cgctggcggc ggtcctcggc ttcgctaccg
     1921 agctctccga ctcctgggac catttcgagc ccgaggaggc gcgttcgctg gtgcgtctca
     1981 tcgccaccca atcggccgat atctcgagca gcgtggacga cctgctcacc gtgacccgcc
     2041 tcgaggcggg cacgatgagc atctacccca ctgcgatcga cgttgcccaa cacctcgcgg
     2101 acatggtcga gacatcgggg cgcgaatcgg ggagggtcat cgactggaaa ggcaacgccc
     2161 tggtctgggc cgaccccacc cgactccggc agatcgtccg caacctgatc acaaatgcga
     2221 tccgat
//
LOCUS       DATITW010000323         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_176176, whole genome shotgun sequence.
ACCESSION   DATITW010000323 DATITW010000000
VERSION     DATITW010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176176"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..587
                     /locus_tag="VLS86_04545"
     CDS             195..587
                     /locus_tag="VLS86_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSO49792.1"
                     /translation="MSVTLEEWIESYRRAWEERDAEAAAALFTPDATYRSNIFEDPHR
                     GKEGVRAYWESVTSTQSDARVRMGRPFADGDRVALEFWTNMKVEGEDVTLPGCLLLDF
                     DDDGQCRQLREYWHFEPGTVEPPTGWGT"
     gene            704..>1681
                     /locus_tag="VLS86_04550"
     CDS             704..>1681
                     /locus_tag="VLS86_04550"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006939923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSO49793.1"
                     /translation="MLATNERRPASTQESTPEIDELKARVGEILNRRPAIGLALGVVR
                     NGSLVFFHGHGMADIASRTPITEDTGFRIASISKTFTAVAVMQLWEQGLVDLDAPAND
                     YLRGYRLIPARAGHRPATVRHLLTHTAGVPQTVHPSQALTSGWFGESVDLGRAVPTLA
                     EYYREGLRLAVEPGTTFTYTDHGFATLGQIVEDVSGEPLAGYFREHIFQGLGMTDTDL
                     NRSRLVESRLATGYTLGRKGAKAVIDRVWVTAAASSIYSTPRDMARYLAALTGGGGNQ
                     SGSMLKPETLTMMFQPHYQPDPRVPGIGLSFFRVDLGGHPAVEHQGILPG"
BASE COUNT          308 a          549 c          556 g          268 t
ORIGIN      
        1 cgtcggcggg cccgagcagg agaaggggga gggtctggct gccttcgccg cgcacacggg
       61 cgaggtgacg gagggatccc acgccgcctg actcctggag ttgatgcgcc ggcaggatgc
      121 gtagctccga atggtggctc gttcgggctt cggaagcgtg accgataccc tggctccacg
      181 agaagggagc actgatgtcg gtgacgctgg aagagtggat cgagagctat cggagggctt
      241 gggaagagcg agatgccgag gcggcggccg ccctgttcac gccggatgca acctaccgct
      301 ccaacatctt cgaggacccg caccggggca aagaaggtgt ccgggcctat tgggaatcgg
      361 tgaccagcac ccagagtgac gcccgggtgc gcatgggtcg tcccttcgcc gacggcgacc
      421 gggttgccct cgagttctgg accaacatga aggtggaggg agaggatgtc acccttcccg
      481 gttgcctcct cctcgacttc gacgacgatg ggcagtgccg tcagctaagg gagtactggc
      541 atttcgaacc gggaaccgtc gagcccccaa ccggatgggg gacgtgaccg agcggcggga
      601 ccaacccgga taagggcaag ctcccctggc cgctgcgcaa agggccgatc gcctctagcc
      661 ctcctttcga cgtcgcgaca acctcgggct ggaggtttca gaaatgctcg cgaccaatga
      721 gcgacgccca gccagcacac aggaatccac gcccgagatc gatgagttga aggcaagagt
      781 cggcgagatc ctcaaccgtc ggcccgcaat cggtctggca ctgggagtgg ttcgcaacgg
      841 atcactggtg ttcttccacg gccatgggat ggccgacatc gcgtcgagga cgccgataac
      901 cgaggacacc gggtttcgga tcgcctccat ctccaagacc ttcaccgcgg tcgcggtgat
      961 gcagttgtgg gagcaggggt tggtcgacct cgatgccccg gccaacgact acctgcgcgg
     1021 ctaccggctg atccctgcca gagcaggcca ccggccggcg acggtgcgac acctgcttac
     1081 ccacaccgcc ggcgtcccac agacggtgca cccctcgcag gcgctcacga gtggctggtt
     1141 cggggagagc gtcgacctgg gtcgggcggt gcccactctc gccgagtact accgcgaagg
     1201 acttcggctg gcggtcgagc cgggcaccac gttcacctac accgaccacg ggttcgccac
     1261 cctgggtcag atagtcgagg acgtgagcgg cgagcccctc gccgggtact tccgggagca
     1321 catattccag ggcctcggaa tgaccgacac cgacctgaac cgatccagac tggtcgagtc
     1381 gcggctggcc accggataca ccctgggccg caaaggcgcc aaagccgtga tcgaccgggt
     1441 gtgggtgacc gcggcggcgt cctcgattta ctccactcca agggatatgg ctcgatacct
     1501 ggcggccctc acaggtggtg gcggcaacca gtctggttcc atgctcaaac ccgagaccct
     1561 gaccatgatg ttccagcccc actaccagcc cgatccccgc gtccccggca tcgggttgag
     1621 cttcttccgg gtcgaccttg gcggccaccc cgccgtcgaa catcagggga tcctccctgg
     1681 g
//
LOCUS       DATITW010000324         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_376626, whole genome shotgun sequence.
ACCESSION   DATITW010000324 DATITW010000000
VERSION     DATITW010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376626"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1835)
                     /locus_tag="VLS86_04555"
     CDS             complement(<1..1835)
                     /locus_tag="VLS86_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HSO49794.1"
                     /translation="MTQEAVLAERAAIDAEVEGKTIIDYLNRNADRYGDQPALHFKEE
                     GEWSRLTWAEYRRAIHEAAAGLQDLGVGDGEFVAIMAGNRPEHVIADYAAIHAGATAV
                     TIYSTLAASQIQYIADNCKATVAVIEDLEFMKRWEEIRSELPNLRYVVLLSGAENYDT
                     AEWVLSWDQLIARGKNRLASDPGSVTRTAGAISPQTLATLIYTSGTTGTPKGVMISHR
                     NVVWTAECLRRAADIDMGPRMVSYLPLAHIAERLATHYLATYMAGQVWYCPNLAGVLE
                     YILEARPTLFVGVPRVYEKFHARLSSRFEEAHGIKKTLLDRALAANKKRVLAEQEGKS
                     GPMLAGLLDSIVLSKVREGLGMDQVSIAITAAAPINPDLILFFQTLGIPLFELYGMSE
                     TTGPGTTNVPGAFRIGSVGRALPGVEVELADDDELLMRGGVITDGYYKLPDDTRESFD
                     PDGWLHSGDLARIDEDGYVWIIGRKKEIIITAGGKNIAPAKLETALGNHPLVSKACMI
                     GDGRKFLSMIIAIDHEEAPAWAAANGLEYHDLASFSELPEVRAEVERAVRAANETVSP
                     VEQVKKWIIVPDEWTPDSGEVTPSLKLKRRVVVDKYSDEIDALYAG"
     gene            complement(1850..>2656)
                     /locus_tag="VLS86_04560"
     CDS             complement(1850..>2656)
                     /locus_tag="VLS86_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018528801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HSO49795.1"
                     /translation="YGRMTGWGQDGPLAGTAGHDIDYIALSGALHAVGTPSTPLPPAN
                     LVGDYGGGGMLLAVGLLAGLIRARSTGEGQIVDAAMLDGSALLTTSMHGYIAEGFWTQ
                     QRGANLLDGGAPFYSVYETADGGHMAVGAIEPQFFAVLLEALEIPPDSVGAQSDRERW
                     PEMRSRLAARFLERSRDEWARLLQGLDACVAPVLTPSEAPRHQHNMERRVFVEVEGLL
                     QPAPAPRFSATPAAVPTGPVYPGRDTDDILASLGLTQDDTSKLRQVGAVA"
BASE COUNT          438 a          861 c          871 g          486 t
ORIGIN      
        1 cccgcgtaca aagcgtcgat ctcgtcggag tacttgtcga ccaccacccg gcgcttgagc
       61 ttgagcgacg gggtcacctc gccggagtca ggagtccact cgtcggggac gatgatccac
      121 ttcttcacct gttccaccgg cgacacggtc tcgttggcgg cccggaccgc gcgctccacc
      181 tccgccctca cctcggggag ctccgagaag ctggcgagat cgtggtattc caggccgttg
      241 gccgcggccc aggcgggagc ctcctcatgg tcgatggcaa tgatcatcga gaggaacttg
      301 cgaccgtctc cgatcatgca ggccttggag accaatgggt ggttgccgag agcggtctcc
      361 agcttggccg gggcgatgtt cttgccgccg gcggtgatga tgatctcctt cttgcggccg
      421 atgatccaga catagccgtc ctcgtcgatc cgggccaggt cgccggaatg gagccacccg
      481 tccgggtcga agctctccct ggtgtcgtcg ggaagcttgt agtagccgtc ggtgatgacc
      541 ccacctctca tcaacagctc atcgtcgtcc gccagctcga cttcgacccc ggggagcgct
      601 ctgcccaccg atccgatccg gaacgcgccc gggacgttgg tggtccccgg cccggtggtc
      661 tcggacatcc cgtagagttc gaagagcggg atgccgaggg tctggaagaa gaggatgaga
      721 tcggggttga tcggagccgc ggcagtgatc gcgatcgaca cctggtccat cccgagacct
      781 tctcgcacct tggagaggac gatcgagtcg aggagaccgg ccagcatcgg gcccgacttc
      841 ccttcctgtt cggccaagac ccgcttcttg ttggcagcga gagcccggtc gagcagggtc
      901 ttcttgatcc cgtgagcctc ctcgaagcga ctgctgagcc gtgcatggaa cttctcgtag
      961 acccggggga cgccgacgaa gagcgtgggc cgcgcctcga ggatgtactc gaggacaccg
     1021 gccagattgg ggcagtacca gacctgtccc gccatgtagg tggccaggta gtgagtggcg
     1081 agacgctccg cgatatgggc cagcgggaga taggacacca tgcgagggcc catgtcgatg
     1141 tcggcggctc gtctcaggca ctcggcggtc cagaccacgt tgcgatggct gatcatcacc
     1201 cctttcgggg tgccggtcgt ccccgaggtg tagatcagag tggccagggt ttgcggtgag
     1261 atcgctcctg cagttctcgt caccgatccc gggtctgacg ccaggcggtt cttgcccctg
     1321 gcgatcagct gatcccagct caagacccac tcggcggtgt cgtagttctc ggcgccggac
     1381 agtaggacga cgtagcgaag attgggaagc tcggagcgga tctcctccca gcgcttcatg
     1441 aactcgaggt cctcgatgac ggcgacggtt gccttgcagt tgtcggcgat gtactggatc
     1501 tggctggcgg cgagggtgga atagatcgtg actgccgtcg ccccggcatg gatcgcggcg
     1561 taatcggcta tcacgtgctc cggacggttc ccggccatga tcgctacgaa ttccccgtca
     1621 ccgacgccga gatcctggag gccggcggcc gcttcatgga tcgcgcggcg atactcggcc
     1681 caggtcaagc ggctccactc cccctcctcc ttgaaatgga gggcgggctg atctccatag
     1741 cggtccgcgt tccgattgag gtaatcgatg atcgtcttac cctcaacctc agcgtcgatt
     1801 gctgctctct ccgccaacac ggcttcttgg gtcatcttcc ctccgggtat caggccactg
     1861 ctccgacctg acggagctta cttgtgtcgt cctgggtaag accgagagag gccaggatgt
     1921 cgtcggtgtc gcggcccggg tagacgggcc cggtaggcac cgccgcaggc gtggccgaga
     1981 accggggtgc gggcgccggc tggaggagac cctcgacctc aacgaagacc ctgcgctcca
     2041 tgttgtgctg atgccgcgga gcctcgctcg gggtgaggac cggggcgaca caagcatcca
     2101 atccctgcag caggcgggcc cattcgtcgc gtgagcgctc gaggaacctg gccgccagcc
     2161 tcgatctcat ctcgggccag cgctctcgat cactctgggc cccgaccgaa tcgggcggga
     2221 tctccagcgc ttcgagcaga acggcgaaga actggggctc gatggctcct actgccatgt
     2281 gccctccgtc ggcggtctcg tacaccgaat agaagggcgc tccaccgtcg agcaggtttg
     2341 ccccccgttg ctgcgtccag aaaccctcgg cgatgtagcc gtgcatcgag gtcgtgagca
     2401 gggccgagcc gtcgagcatc gccgcatcga ctatctgacc ctcaccggtc gatcgggccc
     2461 gaatcaaccc ggcgagcaga cctaccgcaa gcagcatgcc tccgcctccg tagtcaccta
     2521 ccaggttcgc cgggggaaga ggggtggacg gggtcccgac cgcgtgcaac gccccggaga
     2581 gggcgatgta gtcgatgtcg tgccccgcgg tgccggcgag ggggccgtcc tggccccaac
     2641 cggtcatccg cccgta
//
LOCUS       DATITW010000325         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_697463, whole genome shotgun sequence.
ACCESSION   DATITW010000325 DATITW010000000
VERSION     DATITW010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697463"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VLS86_04565"
     CDS             <1..226
                     /locus_tag="VLS86_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HSO49796.1"
                     /translation="TAVRNPGGRTELRPVIASTLVIAGHPVEALINLTGRDEMGYRML
                     LGRRTLRNRFVVDPGKSYLGQRPVRSEEIE"
     gene            223..1179
                     /gene="rimK"
                     /locus_tag="VLS86_04570"
     CDS             223..1179
                     /gene="rimK"
                     /locus_tag="VLS86_04570"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015151713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HSO49797.1"
                     /translation="MKIAILSRRTSLYSTSRLREAAVERGHEVSIVDYLRCYMDITAR
                     RPKVLYRGEALNPEAVIPRIGATYTYYGAAVVRQFEMAGIYSVNESQAISRSRDKLRS
                     LQLLSRAGVGLPTTGFAHATQDIGGLLKVAGGPPVVVKLLEGTQGLGVVLAETTKAAE
                     SVIGAFRQLDANILVQEFIKEAGGADIRAFVVGGRVVAAMKRQAPPGEFRSNLHRGGT
                     AEAIKLTPIQRSMAVRAARTMELNVAGVDLIQSADGPLVIEVNSSPGLEGIETASGVD
                     VAGEIIEFIENNAGLETPRGRSRAGDTQRSDHCRRPGGGTRP"
     gene            1238..2113
                     /locus_tag="VLS86_04575"
     CDS             1238..2113
                     /locus_tag="VLS86_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006100498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HSO49798.1"
                     /translation="MAVINGKRPGPHVWLSGAIHGDELNGVEIVRKVLRLLDAKTLRG
                     AVIAVPIVNPLGFIIESRYLPDRRDLNRAFPGSPRGSTASRLAHLFMREVVSQCSVGI
                     DLHTASNHRINIPQVRADTNDATTLRLARAFGAPFIIHARHRDGSLRQAATELGATVL
                     VYEAGQAHRFDDDAIETGVMGVMRTLRFMKMIDVRLPRVRPTRVMRRTRWVRARRGGL
                     ANITVDLGDEVVKGEVVATISDAFSTRPTAATASASGFVVARTLRPLVNSGDALVHIA
                     SERGPYLDEPAERRR"
     gene            complement(2110..2388)
                     /locus_tag="VLS86_04580"
     CDS             complement(2110..2388)
                     /locus_tag="VLS86_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HSO49799.1"
                     /translation="MMELSMFDVKFSPWREGAACIDVVDEVSFFPDPEDLVGIARAKA
                     VCAGCPVADECLTWSIETNQSEGIWGGHTPKERRSVRRRWLEEIEKAS"
BASE COUNT          475 a          822 c          803 g          443 t
ORIGIN      
        1 aacggccgtc cgcaatcctg gaggccgcac cgagttgcgt cccgtgatcg cgtcgacgct
       61 ggtgatcgca ggccacccgg tcgaggccct gatcaatttg accggtcggg acgagatggg
      121 ataccggatg ctgctcggtc ggcgcacctt gagaaaccgg tttgtggtag accccggcaa
      181 gagttatctc gggcaacggc ccgtgcgcag tgaggagatc gagtgaagat cgccatcctc
      241 tcccgacgaa cgagtctcta ttcgacgagt cgcctccgcg aggcggcggt cgagcgcggc
      301 cacgaggtca gcattgtcga ctatctccgc tgttacatgg acatcacggc ccgccggcct
      361 aaggtcctct accggggaga agcactcaat cccgaggcgg tgatcccgag gatcggcgcc
      421 acctacacct attacggcgc tgcggtggtc cgccaattcg agatggcggg catctactcg
      481 gtgaatgagt ctcaagcgat cagccgatcc cgcgacaagc tgagatcact tcagttgttg
      541 tcgcgagcag gagttgggct ccccacgacc gggttcgccc acgccaccca ggacatcggc
      601 ggattgctca aagtggcggg gggccctccc gtggtggtca agctgctcga aggcacccag
      661 ggcctcggtg tggtcttggc ggagaccacc aaggcagcgg agtcggtgat cggggccttc
      721 cgacagctcg acgccaacat cctggttcag gagttcatca aggaggccgg aggggccgat
      781 atcagggcgt tcgtcgtcgg agggagggtg gtcgcggcga tgaagcgaca ggcccctccc
      841 ggggagttcc gcagcaacct gcaccgtgga ggaaccgccg aggcgatcaa gctgaccccg
      901 atccagcgtt ccatggcggt gcgcgccgcc aggacgatgg aattgaacgt ggccggtgtc
      961 gacctcatcc agtcagccga tggaccgctg gtcatcgagg tcaattcttc cccaggcctc
     1021 gagggcatcg agactgccag cggcgtcgac gttgccggcg agataatcga gttcatcgag
     1081 aacaacgccg gactggaaac accgcgtggt cgctcgcggg ccggagatac gcaacgatcc
     1141 gatcactgtc ggcggccggg tggtggcacc cggccgtagg gcgaggatcg agcttccgtt
     1201 cgccaaggtt cccaccggct cccaggagtc gctaccggtg gccgtgatca atggaaaacg
     1261 cccggggccc cacgtctggc tctcgggcgc gattcacggc gacgagctga acggtgtgga
     1321 gatagtgcga aaggtgttac ggctcctcga cgccaagaca ctccgcggcg cggtgatcgc
     1381 tgtccccatc gtcaaccctc tcgggttcat catcgagtcg cgctatctgc cggatcgacg
     1441 tgacctgaat cgtgccttcc cggggtcccc gcgcggatcg accgcatccc ggctcgccca
     1501 tctcttcatg cgggaggtgg tcagccaatg ctcggtcggc atcgatctgc acaccgcctc
     1561 gaatcatcgc atcaacatcc cacaggtccg cgccgacacc aacgacgcca cgaccctgcg
     1621 gctggcgcgg gcttttggag ccccattcat catccacgcc cggcatcgcg acggatcgct
     1681 gcgtcaggcg gcaaccgagt tgggggcaac ggtgctcgtc tacgaggcgg gtcaggccca
     1741 tcggttcgat gatgacgcga tcgagaccgg ggtgatgggt gtgatgcgaa ccctgagatt
     1801 catgaagatg atcgacgttc gcctgccccg agtcaggccg acccgggtga tgagacgaac
     1861 ccgctgggtc cgggcccgac gtggcgggct ggccaacatc acggtggatc tcggtgacga
     1921 agtggtgaag ggagaggtgg tcgccaccat ctccgacgcc ttttccaccc gacccacggc
     1981 tgctaccgct tcggcgagtg ggtttgtggt ggcccggacc ttgcgcccac tggtgaactc
     2041 gggtgacgcc ctggtccaca tcgcatccga acggggcccc tatctcgacg agcccgccga
     2101 aagaagacgt taggacgctt tctctatctc ctcgagccag cgacgacgca ccgagcggcg
     2161 ttccttgggg gtgtgcccgc cccatatccc ttcgctctga ttggtctcga tcgaccaggt
     2221 gagacactcg tcggcaaccg ggcaaccggc gcaaaccgcc ttggcccggg caatccccac
     2281 caggtcctcc gggtcgggga agaacgacac ctcatcgacc acatcgatac aggcggctcc
     2341 ttccctccag ggagagaact tcacgtcgaa catcgatagt tccatcattt cacgaatcgg
     2401 gtaaacgcct catcaacctc attcgttccc gaatccaaaa tggatgagac ctcgctcccg
     2461 accgaatcca gcttacggtg taattccagg cggtcaaaaa gagtcacggg ccggttgccc
     2521 ccgggcccaa ccgccccagt tca
//
LOCUS       DATITW010000326         4484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_96798, whole genome shotgun sequence.
ACCESSION   DATITW010000326 DATITW010000000
VERSION     DATITW010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96798"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VLS86_04585"
     CDS             <1..1147
                     /locus_tag="VLS86_04585"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HSO49800.1"
                     /translation="PVSAWEHSRLVDMPAGIHRHPVVFVAYDEGVFVIKEVPRRVANG
                     EYQLLRALEERTTRSAEPVGLVERVWLDPLEEQSAAVITRYVDHSFPYRHLVTGSGFG
                     PRRTQLLDAVAGLLVELHLAGCYWGDCSLSNVLYRYDAETIEAVMIDGETSRLRDKLS
                     KGQRKEDLEIMKMNLAGEMADIAAMNGMEIDHADLYLGDDIEARYRSLWSELTQELVI
                     TRDEGYRIRERIARLNDLGFSVGDVYLDPTDAGNVVTMKVSVGGRTFHSERLRQLTGI
                     DASENQARVILSDLNYFLAKHGAMTPTGKSVGTFKWLTTSFEPIVARIRETKTGDPVQ
                     GYCDFLNHRIRLAQARSQDIPNDEALADWIASGLPGFDLQDEIEPVG"
     gene            complement(1144..1614)
                     /locus_tag="VLS86_04590"
     CDS             complement(1144..1614)
                     /locus_tag="VLS86_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49801.1"
                     /translation="MFSYFPYAASFIDGEEGVTVDPALLGVALVVAPFVFVVVALVSR
                     NARAPRQVLLAMGLLLGLGLSIGLISPILGASAGFGVGVAITLKRPDIPDQLRRRLVA
                     VIAAVVYTTLLLFIAPPAAVVTGAVVPILMVGFADEYGAWRWARDHQAPSPQQT"
     gene            complement(1698..2603)
                     /locus_tag="VLS86_04595"
     CDS             complement(1698..2603)
                     /locus_tag="VLS86_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017324308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSO49802.1"
                     /translation="MTAVTETRAPSQPPAPHPAKDGSGTSTVPIYLVVALVTLLWTIP
                     SLGLLINSFREPSSIRGSGWWTVFGEFFSSATWTIQNYTEVLERGFGNAFANTLAVTI
                     PSVVIPLTVAAFAAYAFAWMEFPGRNLIFVAVVGLLVVPLQMALIPILRIYTAIDVNG
                     TFPAVWLAHAGFGLPLAVYLLRNYMGSLPASLIESAKIDGADHFLIFTRLIIPLSVPV
                     LASFAIFQFLWVWNDFLVALVFLGVDPANRVLTVELADLAGRGEGALNLLPAAAFVTM
                     ILPLLVFFSLQRFFVRGLTAGSVKG"
     gene            complement(2600..3739)
                     /locus_tag="VLS86_04600"
     CDS             complement(2600..3739)
                     /locus_tag="VLS86_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSO49803.1"
                     /translation="MSELKEVQKQSVPPEPGAGDNSIQVWLLRIASALVVPGVLVLFF
                     FTFDFLRQEDINKVLQVAIAVVVGVGGIWVLYWGMDRAISAMPERAGSAVRPFAFAGP
                     AMVLLAFYLVYPAVNTFILSFQNSAATEFVGFENFQRIFTESVYLISIRNSVVWVLFV
                     PAVAVGIGLGFATLADKLPRRSESAAKSLIFLPLAISFVGASVVWTFVYDFRTEGFGE
                     QIGILNAILVGFGADPVNWMQVPLWNNLFLMIILVWLQTGFAMVILSSAIKSVPEDQI
                     EASRIDGANEWQVFRKIVVPSIASTIVVVWTTILITTWKVFDIVWVMTGGGFDTSVVA
                     ERMVTEFFTFNNDGMGAALAVLLFVAVVPILVINVKRFREQEATR"
     gene            complement(3879..>4484)
                     /locus_tag="VLS86_04605"
     CDS             complement(3879..>4484)
                     /locus_tag="VLS86_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49804.1"
                     /translation="EGFVLGGPENVAGINFEDSPAPLFSDPAGCAMLKQGSFIANFFN
                     QAGDYEDGEESEIGVFAFPTIDGNTGAMGGGDTLIVFDGAPENVAAIKDWITPEWQCT
                     LASASGGGVAPHGGHGVTGVERLPGHKDVDPNCYESDSAKTFAEAITTALGENTFVFD
                     ASDLMPPEVGQETFWTGMIDWTQGAASQDVTDAIEASWPAG"
BASE COUNT          900 a         1383 c         1462 g          739 t
ORIGIN      
        1 gccggtgtcg gcctgggagc actcccgtct cgtggacatg ccagcgggca tccatcgtca
       61 cccggtggtg ttcgtcgcct atgacgaggg tgtgttcgtg atcaaagagg tgccccggcg
      121 cgtggccaac ggtgagtacc agttgctgcg cgctttggag gagcggacca ccaggtccgc
      181 cgagccggtc ggtctggtgg agcgcgtctg gctcgacccg ttggaagagc agtccgcagc
      241 ggtgatcacg cgatacgtcg atcattcctt tccctaccgg catctggtga cgggctcggg
      301 gttcgggccg aggcgaaccc aactgctgga cgcagtcgcc gggctgctgg tcgagctcca
      361 tctcgccggc tgctactggg gcgactgctc gctatccaat gtgctgtacc ggtacgacgc
      421 cgagaccatt gaagcggtga tgatcgacgg tgagacctcg cggctacggg acaagctctc
      481 caagggccag cgcaaggagg acctcgagat catgaagatg aacctggcgg gggagatggc
      541 ggacatcgcg gcgatgaacg ggatggagat cgaccacgcc gacctgtacc tcggggatga
      601 catcgaagcc cggtaccggt cgttgtggtc cgagctgacc caggagttgg tgatcaccag
      661 agacgagggc taccggatcc gggagcggat cgcccggctc aatgatctcg ggttctccgt
      721 gggagacgtc tatctcgacc ccacagacgc tgggaatgtg gtgactatga aggtctcggt
      781 gggcggtcgg accttccact ccgagaggct ccgccagctc acaggtatcg atgcctcgga
      841 gaatcaggct cgggtgatcc tcagtgatct caactacttt ctggccaagc atggcgcgat
      901 gacaccgacg ggaaagagcg tcggcacttt caagtggctg acgacatcgt tcgagcccat
      961 cgtcgcccgg atcagggaga ccaagaccgg tgatccggtc cagggctact gcgacttcct
     1021 caaccaccgc atacgactcg cccaggcgcg cagccaggat atccccaacg acgaggcgtt
     1081 ggcagactgg atagcgtccg ggttgccggg gttcgacctg caggacgaga tcgagccggt
     1141 cggttaggtc tgttgcgggc tcggcgcctg gtggtcgcgg gcccagcgcc aggctccgta
     1201 ctcgtcggcg aaaccgacca tcaggatcgg gaccaccgcg ccggtgacca cggccgccgg
     1261 aggggcgatg aacaagagca gtgtcgtgta aaccacggca gcaatcaccg ccaccagcct
     1321 gcgtcgcaat tggtcgggga tgtcgggccg cttcagcgtg atggcaactc cgactccgaa
     1381 cccggcactc gccccgagga tcggggagat gaggccgatc gacaacccga gaccaagcag
     1441 cagtcccatg gccagcagga cctggcgggg ggcgcgggcg ttcctcgaga ccagggccac
     1501 caccacgaac acgaacggtg ccaccaccag ggcgaccccg agaagcgcgg ggtcgacggt
     1561 gaccccttct tcgccgtcga tgaaggaagc cgcgtagggg aagtagctga acatgccgac
     1621 aaccgtgccc agggcgaggg cgagccagtt gcggccccgg atcttgtccc agctgtctga
     1681 gttggccagc gtgtgcctta tcccttgacg gagccggcgg tgaggcctcg gacgaagaac
     1741 cgctgcagtg agaagaacac caggagaggc aggatcatcg tcacgaaagc cgccgcgggg
     1801 agcaagttga gggcgccctc cccacgtccg gccagatcgg cgagctcgac tgtcaacacc
     1861 cggttcgccg ggtcgactcc gaggaacacc agggccacca ggaagtcatt ccatacccag
     1921 agaaactgga agatggcgaa cgaggcgagc accgggaccg agagcgggat gatcagccgg
     1981 gtgaagatca agaagtggtc ggccccgtcg atcttcgccg actcgattag ggacgccggt
     2041 aacgagccca tgtagttgcg cagcaggtac accgccagcg gaagcccgaa cccggcgtgg
     2101 gccagccaca ccgcggggaa cgttccgttc acatcgatcg cggtgtaaat acgcaggatc
     2161 gggatcagag ccatctgcag gggcacgacc aacagcccga ccaccgccac gaagatcagg
     2221 ttgcggccgg ggaactccat ccaggcgaag gcgtaggcgg cgaaggcggc cacggtcagt
     2281 gggatgacca ccgaggggat ggtgaccgcg agcgtattgg cgaatgcgtt gccaaagcct
     2341 ctctccagca cttcggtgta gttctggatg gtccaggtag ccgagctgaa gaactcgcca
     2401 aacaccgtcc accatcccga gccccggatc gagctcggct cccggaacga gttgatcagc
     2461 aagccgagcg acgggatggt ccacaacagg gtgaccaggg ccactacgag gtagatgggg
     2521 accgtcgatg tgccagatcc gtctttggct gggtggggcg ccggcggctg ggacggggcg
     2581 cgggtctcgg tgaccgctgt catctggtcg cctcctgctc tctgaaccgc ttgacgttga
     2641 tgaccaggat tgggaccacg gccacgaaca acagcacggc gagcgccgct cccatcccgt
     2701 cgttgttgaa ggtgaagaac tcggtcacca ttcgctcggc gacgacggag gtgtcgaacc
     2761 cgccgccggt catcacccag acgatgtcga acaccttcca ggtggtgatg aggatggtgg
     2821 tccataccac cacgattgtc gaggcgattg agggaacgac gatcttccgg aacacctgcc
     2881 attcgttggc gccgtcgatg cgtgaggcct cgatttgatc ctcgggcacg ctcttgatcg
     2941 cggaggagag gatcaccatg gcgaatccgg tctgaagcca gacgaggatg atcatcagga
     3001 acaggttgtt ccacagtgga acctgcatcc agttcacggg atcggccccg aatccaacca
     3061 ggatcgcgtt caggatcccg atctgctcgc cgaacccttc ggtgcggaag tcgtagacga
     3121 aggtccacac cacggaagcc ccaacgaacg agatggccaa gggaaggaag atcaaagact
     3181 tggcggccga ctcggatctt ctcgggagct tgtcggccaa tgtggcgaat ccgagcccga
     3241 tgcccacggc gaccgccggc acgaacaaca cccagacgac gctgttcctg atcgagatca
     3301 gatagacgct ctcggtgaag attctctgga agttctcgaa cccgacgaac tcggtggcgg
     3361 cggagttctg aaagctgagg atgaaggtgt tgaccgccgg atacaccaaa tagaaggcca
     3421 gcagaaccat tgccgggccg gcgaacgcaa agggtcggac cgcagacccg gcccgttccg
     3481 gcatggcgga gatcgctcgg tccatccccc agtacaggac ccagatcccg cccactccga
     3541 ccacgacagc aattgccacc tggaggacct tgttgatgtc ctcctgcctc aggaagtcga
     3601 aggtgaagaa gaacaacacc aggacgccag gcacgaccag ggcgctggcg atgcgaagca
     3661 gccacacctg gatggagttg tcgccggcgc caggctcggg tggcacagat tgtttttgaa
     3721 cctctttgag ctcactcacc ggaggagtgg tcatcgtgcc tccttcagtc ttgtgaccat
     3781 gccgagcccg accctaccgc accgcacaag gcgatgccgg gtgttcacgc agagaggggt
     3841 ggtcccgtga ccacccctct ctgcttcagg ctgtcttctc agccggcggg ccaactggcc
     3901 tcgatggcgt cggtcacatc ctggctagcg gcaccttgcg tccaatcgat catcccggtc
     3961 cagaaggtct cctgacctac ctcggggggc atcagatcgg aggcgtcgaa cacgaaggtg
     4021 ttctcgccca gcgcggtggt gatcgcctcg gcgaaggtct tcgccgagtc gctctcgtag
     4081 cagttggggt caacgtcctt gtgaccgggg agtcgctcca cccctgtcac accatgaccc
     4141 ccgtgcgggg ccacgccgcc accggaggcg gaggcgaggg tgcactgcca ttcgggtgtg
     4201 atccagtcct tgatcgcagc cacgttctca ggcgctccgt cgaagacgat gagggtgtcg
     4261 ccaccgccca ttgccccggt attgccgtcg atggtgggga aggcaaagac gccgatctcc
     4321 gactcctcgc cgtcctcgta gtcaccagcc tggttgaaga agttggcgat gaacgagccc
     4381 tgcttcaaca tcgcacatcc cgccgggtca ctgaacagtg gcgcagggga gtcctcgaag
     4441 ttgatcccgg ctacgttctc ggggccaccg agcacgaatc cttc
//
LOCUS       DATITW010000327         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_336819, whole genome shotgun sequence.
ACCESSION   DATITW010000327 DATITW010000000
VERSION     DATITW010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336819"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /gene="metG"
                     /locus_tag="VLS86_04610"
     CDS             <1..1582
                     /gene="metG"
                     /locus_tag="VLS86_04610"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HSO49805.1"
                     /translation="GHLGGAYLPSDIFARYHRIAGNDVLMVSGSDAHGTPITVKADEE
                     GMTPGQVVEKYHAEILGYWKALGISFDLFTTTMTENHARVTQDVFLRLLEKQYLVKGT
                     WTQYFDPEAERFLPDRYVEGTCPHCGYAEARGDQCENCGRTLDASDLIAPRSRLTGAT
                     PVPKETEHFFFKLPEFAEPLREWLESKTTWRRHVKNWALGMIDEGMPERAITRDIEWG
                     VEVPVDDLGPGKRIYVWFDAVIGYLSAAKEWAASTDDPDAWKRWWEDPAAESYYFVGK
                     DNIPFHAVIWPAMLMGYGGLNLPTDVPANQYITFKGKKASKSQGVGRSMGWYAERLEP
                     DAIRYAIAAVLPEQNDTDLSDDEIIRRVNEELVATWGNLVNRVLSISERTFDGQVPMP
                     GPLTAADQALIDGIDRALVVGAAHIEAVELRAGLRSAMDAATEVNAYLNSEEPWRVLK
                     EDRERGATILWTALQAISGIRVALSPYLPWTTGQVGKMLGIRPEVTGWERPPVEGGIK
                     LGRILPLFTKLEPDALDD"
     gene            1653..1922
                     /locus_tag="VLS86_04615"
     CDS             1653..1922
                     /locus_tag="VLS86_04615"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HSO49806.1"
                     /translation="MARLAKIITGFTLIIAGLFMIVLPGPGILTILGGLTLIAQEYVW
                     ARRLSDWVKDRFGRYAGYRPTTPGDAVPDVIPGLGERAAEDQLGG"
     gene            complement(1907..2278)
                     /gene="sodN"
                     /locus_tag="VLS86_04620"
     CDS             complement(1907..2278)
                     /gene="sodN"
                     /locus_tag="VLS86_04620"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HSO49807.1"
                     /translation="MFCGVYDPAQAKIEAMSVLKSAEKYQASDDEVFRDRAILIKEER
                     AELVKHHLMVLWVDFFTADHRKEFPNLDDLFWRAILQAGNAKKSVDPEDGKKLVALVD
                     EISDVFWQTEKAKTAGVYPPS"
BASE COUNT          412 a          729 c          743 g          396 t
ORIGIN      
        1 cggacatctc ggcggcgcct acctcccctc cgacatcttt gcccgctatc accgcatcgc
       61 cgggaacgac gtgctcatgg tctccggctc cgacgcccac ggcaccccga tcacggtgaa
      121 ggccgacgag gagggaatga cgccgggcca ggtggtggag aagtatcacg ccgagatcct
      181 cggctactgg aaggcgctgg ggatctcgtt cgacctgttc acgacgacca tgaccgagaa
      241 ccacgcccgg gttactcagg atgtctttct ccgcctcctg gagaagcagt acctggtcaa
      301 gggaacctgg acccagtatt tcgaccccga ggcggagcgg ttcctccccg accgttacgt
      361 cgaggggacc tgtccccact gcggctatgc cgaagccagg ggcgaccagt gcgagaactg
      421 cggccggact ctggacgcct ccgacctgat cgctcccagg tcacgactca ccggggcgac
      481 gccggtgccc aaggagacgg agcatttctt cttcaagctg cccgagttcg cagagccgtt
      541 gcgggagtgg ctcgagtcga agaccacctg gcgtcgccac gtcaagaact gggcactggg
      601 aatgatcgac gagggcatgc ccgaacgcgc catcacccgg gatatcgagt ggggcgtcga
      661 ggtcccggta gacgacctcg gcccgggcaa gcgcatctat gtctggttcg acgcggtgat
      721 cgggtatctg agcgcggcca aggagtgggc tgcatccacc gacgaccccg acgcctggaa
      781 gcggtggtgg gaagacccgg ctgcggagtc ctactacttc gtcggcaagg acaacatccc
      841 gtttcacgca gtcatctggc cggcgatgct gatggggtac gggggcctga atctccccac
      901 cgacgtcccg gcgaaccagt acatcacctt caagggcaag aaggcgtcca agtcgcaagg
      961 ggtagggcgg tcgatgggtt ggtacgcgga gcgtctcgag ccggacgcca tccgctatgc
     1021 gatcgcggcc gtgctccccg agcagaacga caccgatctg agcgacgatg agatcatccg
     1081 cagggtcaac gaggagctgg tggccacctg gggcaatctg gtcaaccgcg tcctctcgat
     1141 cagtgagcgg accttcgacg ggcaggtgcc catgcccggg cccttgactg ccgccgatca
     1201 agccctgatc gacggcatcg accgggcgct cgtcgtcggg gcggcacaca tcgaggcagt
     1261 cgagttgcgg gcggggctga ggtcggcgat ggacgcagca accgaggtca acgcctatct
     1321 caactccgag gagccgtggc gggtgctcaa ggaggatcgt gagcggggag cgacgatcct
     1381 gtggaccgcg ctccaggcca tctccgggat tcgggtcgcg ctgtcgcctt atctgccgtg
     1441 gaccaccggg caggtcggga agatgctcgg cattcggccc gaggtgacag gatgggaacg
     1501 tcccccggtg gaaggtggca tcaagttggg ccggatcctg ccgttattca ccaagctcga
     1561 gccagatgcc ctggacgact gaaacccggc gacaccgtcg ggggtcgaac cctcgggctc
     1621 acagagcaga cgaagacgat taggttctcg aaatggcgcg gctggcaaag atcatcaccg
     1681 ggttcaccct catcatcgcc ggtttgttca tgatcgtcct gcccggccca gggatcctca
     1741 cgatcctggg tgggctgacc ctgatcgctc aggagtatgt ctgggctcgt cgtctctccg
     1801 actgggtcaa ggatcgattc ggccgatacg ccgggtaccg gcccacgact cccggggatg
     1861 cggtccccga tgtcattccc ggtctcggcg agcgcgccgc cgaggatcag ctcggcggat
     1921 agacccctgc ggtcttcgcc ttctcggtct gccagaacac gtccgagatc tcgtcgacca
     1981 aggccaccag cttcttccca tcctcggggt cgacggattt cttggcgttc cccgcctgga
     2041 gtatcgcccg ccagaacaga tcgtcgaggt tggggaactc cttgcggtga tcggcggtga
     2101 agaagtcgac ccagagcacc atgaggtggt gcttgactag ctcggcgcgc tcctccttga
     2161 tcaggatcgc ccgatcccgg aagacctcgt catccgatgc ctggtacttc tcggccgact
     2221 tgagcacgga catcgcctcg atcttggcct gggccgggtc gtagaccccg cagaacagat
//
LOCUS       DATITW010000328         1846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_417207, whole genome shotgun sequence.
ACCESSION   DATITW010000328 DATITW010000000
VERSION     DATITW010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417207"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1335)
                     /locus_tag="VLS86_04625"
     CDS             complement(112..1335)
                     /locus_tag="VLS86_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49808.1"
                     /translation="MTERTAAKVAWVAGVLVVSLAAAAIFSLFAYPISEGTTIDVGNA
                     ITTILIFASFPTVGALVASRRPRNPIGWLLMGIGALFVLALAMGNYGEYGLRVEPGSL
                     PGAVYAAWVGGWIWPVVLGLVGLPILRFPNGRPPSPRWNWAGRALVGSIMLWVLAAGL
                     VPGPMSNAGFESVDNPLGVADSLRGPLLVASGLAGFGILLSVLACAMSMVVRFRRSRG
                     VEREQVKWLAYAASLIAVLGVIEIVVEGTSPPEIIARISQLALAGSISALPIAVGIAI
                     LRYRIYDIDRLISRTIAYAIVTGLLAAGYAGGVLLLQVALPVSQESSIVVAVTTLAVV
                     ALFGPLRNRVQALVDRRFNRRRYDAQRTLVSYGDRLRSEPDLDILSSDLVGVVTDTMQ
                     PTHVSLWLRHVDAAK"
     gene            complement(1648..>1846)
                     /locus_tag="VLS86_04630"
     CDS             complement(1648..>1846)
                     /locus_tag="VLS86_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49809.1"
                     /translation="AIARSKVFPQWSGILMAVGAPAHLVGFGIAQLESPSLWFVSILG
                     SLLLGSGLAWCGSRMWATSSH"
BASE COUNT          376 a          624 c          576 g          270 t
ORIGIN      
        1 cttctttgtg gtgctcatct cttgacctcc tatacgagtg acaattgaga acctaggggc
       61 gatgcgaggg gaaagcttct cgaatcctgc tcgatgacga gcggctgctc cctacttggc
      121 agcgtccacg tgtcggagcc acagcgacac gtgagtgggc tgcatggtgt cggtcaccac
      181 ccccacgaga tcgctcgaca ggatgtcgag gtcgggctcc gagcgcaaac ggtcgccgta
      241 gctgaccaac gtgcgctggg cgtcgtaacg gcggcggttg aacctacggt cgacgagggc
      301 ctggacgcgg ttcctgaggg gtccgaacag cgccaccacc gccaaggtgg taaccgcgac
      361 cacgatcgag gattcctgtg ataccggaag cgccacctgg agcagcaata cgccgcccgc
      421 gtagccggcc gccagcaagc cggtcacgat ggcgtatgcg atcgtgcggc tgatcaggcg
      481 atcgatgtcg tagatgcggt agcgcaagat cgcgatcccc accgcgatcg gcagcgcaga
      541 aatggagccc gccagtgcca gctgggaaat ccttgcgatt atctcgggcg ggctcgttcc
      601 ctcgaccacg atctcgatca ccccgagcac ggcgatcagc gacgcggcat acgcaagcca
      661 tttcacctgc tcgcgctcga ccccccgtga gcgtcggaag cggacgacca tcgacatcgc
      721 gcaggcgagc acgctcaaca ggatcccgaa tccggcaagt ccgcttgcga cgagcaacgg
      781 cccccgcaac gagtcggcga cgcccaaggg gttgtccaca ctctcgaagc cggcgttgct
      841 catgggtccg gggacgaggc ccgccgccag cacccacagc atgatcgacc ccaccagcgc
      901 cctgccggcc cagttccagc gtggcgacgg gggccgacca ttggggaacc ggaggatggg
      961 cagcccgacg agcccgagca ccaccggcca gatccatcca ccgacccacg ccgcatagac
     1021 cgctcccgga agggacccgg gctccacccg gaggccatat tccccatagt tgcccatggc
     1081 aagtgccagt acgaagaggg cgccgattcc catcaacaac cagccgatgg gattgcgtgg
     1141 gcgccgcgac gcgaccaggg caccgacggt ggggaaggac gcgaagatga ggatcgtcgt
     1201 gattgcgttg cccacgtcga tcgtggtccc ttcactgatc gggtatgcaa agagggagaa
     1261 gatcgcggcc gccgccagcg agaccaccag cacccctgcc acccaggcga ccttggccgc
     1321 ggtccgctcg gtcactcttg ggcctccgac gttctgagcc agagcgaggc gggcgccgac
     1381 gccatgacct acctggcgtc cgcgagtcgg tgggaggagc gcaagctgtc gggcgcgatc
     1441 agacccggac cacgcaccca ctcgatccgg cccgtatcga ggatgaaggg ccatgcttca
     1501 tgccaccggc cagcgcacaa cccgcctgtc atcggagagg ggctcttctc aacggcaagg
     1561 acttgggtgg caaccacttc gttggcgagc atctcaacgc agcgttccga aatcgttccg
     1621 ggccaccatt cacgccctat ttcccgctca atgactcgat gtggcccaca tccgagagcc
     1681 gcaccaagcc agacccgagc cgaggagcag gctgccgaga atggacacga accagagtga
     1741 aggtgactcg agttgggcaa tcccaaaccc caccaaatga gccggggcgc ccaccgccat
     1801 caagatgccg gaccattggg gaaacacttt cgacctggca atcgcg
//
LOCUS       DATITW010000329         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_217012, whole genome shotgun sequence.
ACCESSION   DATITW010000329 DATITW010000000
VERSION     DATITW010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217012"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VLS86_04635"
     CDS             <1..493
                     /locus_tag="VLS86_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSO49810.1"
                     /translation="DRGIRPGLGIDDEQVAPGDMFAQMRATISLQHATAFDLKLAGKP
                     GVPRLMNTRDVIRYATLDGARVAGRSRELGTLEPGKQADIVMLRTDRPNIFPINDPIG
                     AVVWGMDTSNVDWVFVAGRPVMRAGVLEANVEQARDLATTAQHRVATAAGLAPAAAPG
                     GER"
     gene            553..1563
                     /locus_tag="VLS86_04640"
     CDS             553..1563
                     /locus_tag="VLS86_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49811.1"
                     /translation="MPVYIALLLLIIVASIWAPATVSGVALRAIAPYGAVLGITALGQ
                     MLVIMTGGIDLSVPGTMSLAAVIMVGVGDGSNEQIIIAIATAIAAAAVVGLVNGILIG
                     GLKLNPLIVTLAVGLIVVGRVNRYGTTFPVQSPVPTGLSDWTSTRVLGASPVLWIGVA
                     LTLVLIVGLRYTAVGRRFQAVGANPVASHVVGVRVNVHQILVYVVAGVFYAIAGVALA
                     GLIRTPGVSVGSQYLLGPIAAVVIGGASLTGGLASPLSTFAAAFFLTGLNQMLRTMGL
                     PTSLQFVVFGLVIIGGMLVSGDRIVRAVEQLIRERKRPDRAQTPTLPGSAEGRAWKEE
                     GM"
     gene            1560..2939
                     /locus_tag="VLS86_04645"
     CDS             1560..2939
                     /locus_tag="VLS86_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49812.1"
                     /translation="MTVPSFRERQGYRLDGRRNMQRIRIIGVIAVLALTATACGGGGE
                     TTPSDTPAPTDGTGGGLEDFGESLDADPALVEKALGPVEPSDEESWNIILASIARAEQ
                     DLDQATIDKAMECWNSQECDTGTGGEMVMGYADGGGDTVNVWRAVSHMEAILQALTYE
                     EIGTIVSTEANFGGPTFDPTIAENDIRFLINRGVDFIVGYPDIGVGLREDVLEAEEAG
                     IHYIPFSAGYVGLTGQEGALIPGEDYLTVVGEDLCALGGSFADVLNTGVGDGEVALLG
                     GTPNNALSLGWQQCAVTEFADGLELINPPANENTTTGDTFWSPPAIPGVFQGLLTTNP
                     EIKGWAYEYADGMNIGLQTYADLGIPVENLTVALRTDEQDLFCDWADRNEPTYHIFYS
                     AGGNFQSRIGVTAGMLNLKGAEIPAEVVVPHIMREVTADDCDPDRVAPAVSGTSLVPD
                     EVLQLMFAS"
     gene            2939..>3160
                     /locus_tag="VLS86_04650"
     CDS             2939..>3160
                     /locus_tag="VLS86_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO49813.1"
                     /translation="MTGGGPGGASDPGDRVLELRGVSRQFGEVRALTDVSFDCRAGEV
                     HAVVGENGSGKSTLLGIASGFVDPDLGSVQ"
BASE COUNT          533 a         1013 c         1076 g          538 t
ORIGIN      
        1 cgaccgaggt atccggcccg gactagggat cgatgacgaa caggtggctc cgggcgacat
       61 gttcgcccag atgcgggcga cgatctcgct gcagcacgcc acggccttcg atctcaagct
      121 ggccggcaag cccggcgttc ctcgactcat gaacactcga gacgtgatac gctacgccac
      181 gctcgacggg gcgcgggtag cgggacggag ccgggagctc ggaacgctcg agcccggcaa
      241 gcaagccgat atcgtgatgc tgcggaccga ccgcccgaac atcttcccga tcaacgaccc
      301 gatcggcgca gtcgtgtggg gcatggacac ctcgaacgtc gactgggtct tcgtcgccgg
      361 cagacccgtg atgcgcgctg gtgtcctcga agcgaacgtg gagcaagccc gcgacctggc
      421 gacgacggcc caacaccgtg tcgcgaccgc cgccgggctc gctcccgctg ctgcaccggg
      481 gggtgagcgg tgagaacgac gacgaagccc ccctcggcag cgctcacgtc gttcgtcgtt
      541 ggctctcgct acatgcccgt gtacatcgct ctgctccttc tcatcatcgt ggcgtccatc
      601 tgggctccgg caaccgtcag cggtgtagcc cttcgcgcca tcgcgcccta cggggcagtg
      661 ctcgggatca ccgccctcgg acagatgttg gtgatcatga ccggcgggat cgacctcagc
      721 gtccccggga cgatgagcct ggccgcggtg atcatggtcg gggtcgggga cgggtcgaac
      781 gagcagatca tcatcgccat cgccaccgcg atcgccgccg ccgcggtggt cggactcgtc
      841 aacggcatcc tgatcggcgg gctcaagctg aacccactca tcgtcaccct ggcggtgggt
      901 ctgatcgtgg tcggcagggt caatcgctac ggaaccacgt tcccggtcca gagcccggtg
      961 ccgaccgggt tgtccgactg gacgtcgacc cgcgtcctcg gtgccagccc cgtcctgtgg
     1021 atcggcgtcg cgctcaccct cgtcctgatc gtcggcctcc ggtacacggc ggtcggtcgc
     1081 agattccaag cggtgggcgc caaccccgtc gcctcccacg tcgtcggcgt ccgggtgaac
     1141 gtgcaccaga tcctcgtgta cgtggtcgcc ggggtgttct acgccatcgc cggcgtcgcg
     1201 ctggcggggc tgatccgtac cccgggggtt tccgtcggtt cgcagtacct cttgggcccg
     1261 atcgcggcgg tggtgatcgg cggagcctcg ctgactggag ggttggccag cccgttgtcc
     1321 acgttcgccg ccgccttctt cctgacgggt ctgaaccaga tgttgcgaac gatggggctg
     1381 ccgacctccc ttcagttcgt cgtgttcggt ctcgtgatca tcggaggcat gctggtatcg
     1441 ggtgatcgga tcgttcgggc cgtcgagcaa ctgatccgag agcggaaacg cccggatcgc
     1501 gcgcagacgc ccacgctccc cggctccgcc gagggacgcg cttggaagga ggagggtatg
     1561 tgacggttcc atcgttccgg gaacgccagg ggtatcgact cgatgggagg aggaacatgc
     1621 aacgcatcag gatcatcggc gtcatcgccg tgctggcctt gaccgcaaca gcatgtggtg
     1681 gaggtgggga gacgaccccg agtgacacgc ccgccccgac cgatggcaca gggggcggtt
     1741 tggaagattt cggggagtcc ctcgatgccg acccggcatt ggtagagaag gcactcggcc
     1801 cggtggagcc gtcggatgag gagagctgga acatcatcct tgcctccatc gcgcgtgccg
     1861 agcaggacct cgatcaagcc acgatcgaca aggcgatgga gtgttggaac agccaggagt
     1921 gtgacaccgg gaccggcggc gagatggtga tgggctacgc cgacggcggc ggcgacacgg
     1981 tgaacgtctg gcgtgcggtg agccacatgg aggccatcct ccaggcgctc acctacgagg
     2041 agatcggaac catcgtctcc accgaagcca acttcggcgg accgaccttc gatcccacca
     2101 tcgccgagaa cgacatccgg ttcctcatca accggggagt cgacttcatc gtcgggtacc
     2161 cggacatcgg agtcggactc cgagaggacg tcctcgaggc cgaggaggcg ggcatccatt
     2221 acataccgtt ctcggcaggc tacgtcggtc ttacaggtca ggaaggtgcc ctcatccccg
     2281 gtgaggacta cctgacggtg gtcggcgagg atctgtgtgc actcgggggg agcttcgccg
     2341 acgtcctgaa cacgggtgtc ggtgacggcg aggtggcgct cctcggcggg acaccgaaca
     2401 acgccctgtc cctcggctgg cagcaatgcg cggtcacgga gttcgcggac gggctcgagc
     2461 tgatcaatcc gcctgccaac gagaacacga cgaccggaga caccttctgg tcccctccgg
     2521 cgatccccgg cgtgtttcaa gggttgctga cgaccaaccc agagatcaag ggctgggcgt
     2581 acgagtacgc cgacgggatg aacatcggcc tgcagacata tgcggatcta ggcatcccgg
     2641 tggagaacct cacggtcgcg ttgcgcaccg acgagcagga tctgttctgc gactgggcgg
     2701 accgcaacga gcccacatac cacatcttct actcggcagg tggcaacttc cagtcacgga
     2761 tcggggtcac cgcggggatg ctgaatctca agggtgccga gatcccggct gaggtggtcg
     2821 tgccacacat catgcgggag gtgacggcgg acgactgtga tcccgatcgt gtcgccccgg
     2881 ccgtttccgg gacatcgctg gtgccggacg aggtcctgca gctgatgttc gcgagctgat
     2941 gacgggagga ggtccaggag gagcgtcgga ccccggagac cgggtcctcg agctgcgggg
     3001 tgtatcacgg cagttcgggg aggttcgagc gctgaccgac gtcagcttcg actgccgtgc
     3061 cggcgaggtc cacgccgtcg tcggcgagaa cggctccggg aaatcgaccc tcctcgggat
     3121 cgccagtggg ttcgtcgacc ccgatctggg ctctgtccag
//
LOCUS       DATITW010000330         1668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_16746, whole genome shotgun sequence.
ACCESSION   DATITW010000330 DATITW010000000
VERSION     DATITW010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16746"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /gene="pucL"
                     /locus_tag="VLS86_04655"
     CDS             <1..754
                     /gene="pucL"
                     /locus_tag="VLS86_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HSO49814.1"
                     /translation="EGQDDFSDISVQILLEGSVESAHVSGDNTAVLPTDTMRNTVYCM
                     AQDHLTRDLEAFGAVLSDHFLARPEVTGVTVTIAERLWNRIGPTGFVGGGSERRTARV
                     QRGEPGDTVQAGLEGLVVLKTTGSSFAGFPRDQFTVLPEADDRILATSVTATWRYATM
                     PADTTATWDRARQTILDRFFADRSASVQHQGWLMASAVLEAIPEIDEIEFHMPNQHHV
                     PFDVARFGMVHEGTVFHPVSEPYGDIGFTVTR"
     gene            complement(782..>1668)
                     /locus_tag="VLS86_04660"
     CDS             complement(782..>1668)
                     /locus_tag="VLS86_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate kinase"
                     /protein_id="HSO49815.1"
                     /translation="LSGELHRRSLRTLDLFLTAAVENGAGLPDGFTVTLPKIQDLEQV
                     VVLSEALTELEQSLGIEPIPIELMVETPQTIIDRDGKAGVRKFVDAGQGRVRGAHFGT
                     YDYTASLTIPAAYQTMTHPACDVARQALQASLAGSGVWISDGATNVMPVPPHRGDDLT
                     TAQVEENRRTVHDAMRLHYDHIRHSLTHAYYQGWDLHPAQFPTRYAAVFAFFLEGLEQ
                     AGDRLHNFVESAAQATLVGEVFDDAATGQGLLNFFLRAINCGAITEEEATRRSGLSLE
                     EIQTRSFLAILEGRTSGG"
BASE COUNT          271 a          555 c          564 g          278 t
ORIGIN      
        1 cgagggacag gacgacttct cggacatcag cgtccaaatc ctgctcgagg gctcggtcga
       61 atcggcacac gtcagtggcg acaacaccgc ggtgctcccc accgacacca tgcgcaacac
      121 ggtctactgc atggcccagg atcatctcac ccgcgacctg gaggcattcg gtgcggtgct
      181 gagtgaccat ttcctggccc gccccgaggt gaccggggtg acggtgacca tcgcggagcg
      241 gctctggaac cggatcggtc ctaccggatt cgtcggcggc gggtcggagc gtcgcacggc
      301 tcgggtgcag cggggagagc caggagacac cgtccaggcg ggtctcgagg gcctcgtggt
      361 gctaaagaca accggttcgt cgttcgccgg gtttccccgg gaccagttca ccgtgctgcc
      421 agaggccgac gaccggatcc tggccacttc ggtaactgcg acctggcgct acgccacgat
      481 gccggctgac accaccgcca cctgggatcg agcgcgtcag accatcctcg accggttctt
      541 tgcagaccgg tcggcgtcgg tccagcacca gggctggctg atggcgagcg cggtgctcga
      601 ggcgatcccc gagatcgacg agatcgagtt ccacatgccc aaccagcacc atgtgccctt
      661 cgacgtcgca cgattcggga tggtccacga ggggaccgtg ttccatccgg tgtccgagcc
      721 ctatggggac atcggcttca ccgtgacccg ctgaccccgc ccgtcctccg gacggagggg
      781 gtcatccgcc cgaggtccgc ccctccagga tggcgagaaa cgagcgggtc tggatctcct
      841 ccaggctcag gccgctccgc cgtgtcgcct cttcttcggt gatggccccg cagttgatgg
      901 cccggagaaa gaagttgagc agaccctgcc cggtggccgc gtcgtcgaac acctcgccga
      961 ccagggtcgc ctgggccgca gactcgacga agttgtgaag tcggtccccc gcctgctcta
     1021 gtccttcgag gaagaaggcg aagaccgccg cgtaccgggt ggggaactga gccgggtgga
     1081 gatcccatcc ctggtagtag gcgtgggtca gggaatgcct gatgtggtcg tagtgaagac
     1141 gcatcgcgtc gtggacggtg cgccggttct cctcgacctg cgccgtggtc aggtcgtcgc
     1201 cgcggtgcgg tggcaccggc atcacgttgg tcgcgccgtc ggagatccac acccccgagc
     1261 cggccaggct cgcctggagc gcctgccggg cgacgtcaca cgcagggtgg gtcatcgtct
     1321 gataggcggc ggggatcgtc agcgacgcgg tgtagtcgta ggtgccgaag tgcgcccccc
     1381 gcacccgccc ctgcccggcg tcgacgaact tgcgcacccc ggccttccca tctcgatcga
     1441 tgatcgtctg cggcgtctcc accatgagct cgatcgggat cggctcgatc ccgaggctct
     1501 gctccagctc ggtcagggct tcggagagga ccacgacctg ttcgaggtcc tggatcttgg
     1561 gaagggtgac agtgaagccg tcggggagac cggcgccgtt ctcgacggcc gcggtcagaa
     1621 acaggtcgag agtccgcaga ctccgacgat gcagctctcc cgaaagcg
//
LOCUS       DATITW010000331         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_16813, whole genome shotgun sequence.
ACCESSION   DATITW010000331 DATITW010000000
VERSION     DATITW010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16813"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2066)
                     /locus_tag="VLS86_04665"
     CDS             complement(<1..>2066)
                     /locus_tag="VLS86_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49816.1"
                     /translation="YGDGVAYWPLAEILKEVAGIKDTDTSSEALEQIRALGDSVLTQL
                     TDDPGMTVAALAHTMGLTDPDHSFAELDPREVRARTRSAWRSLFTALSEESPVVAVIE
                     DIHWADTALLDILEELTEKVAGPVIIICPARPELTDRRPGWGGGKRNHSSIFLDPLTS
                     EESDALVGYLLSIADLPPTVRLAMMNKAEGNPFFLEEIIRHLIDQGHIVRDGSNWRAV
                     SDSDDVEIPDTVQGVLAARIDLLDPVEKRVLQRAAVVGRVFWPTPVSRLLGSDTNDLG
                     STLDHLEDRELIRSNLGSSLAGEAEFSFKHVLTREVAYDSLPRRERGAAHAAVGAWIE
                     EATRDRGSELVELTAYHWGEAFRAAEEDPRSTSEEIESLRVKAFEATVSASERSRGRA
                     AVHQAHRLADEALLLAREPLERARGLTAKGLAGLSGYAGDIAWFSLKEAADLLIQHAP
                     GERRLIARTCARAVETPTRWPGSMKTLVSEEEVNHYLELGLANLDPGDESEEMVRLLL
                     ARSMGLFSRWKPGDIDTAVVDMTRAAGRRAFEIAESIQRVDLASAALDAVGSVENSLG
                     DYRASSEVIERRLELLDQIANPWEVGDALAMGAWNFGFVGDFSRARALAQRGIDTSDD
                     EAGGLILHNTAWATYAEFCLGNWNRVVSTLAARARLALRGDVSDPPYFSGHQFGIEAF
                     IHTARR"
BASE COUNT          341 a          703 c          662 g          360 t
ORIGIN      
        1 ccgccgcgcc gtgtgaatga aggcttcgat cccgaactgg tgccctgaga agtaaggggg
       61 atcgctgacg tcgcccctca gtgccagccg agccctggcc gccaacgtgg agaccacccg
      121 attccagttg cccagacaga actcggcgta cgtcgcccac gccgtgttgt ggagaatgag
      181 ccctccggct tcgtcatcag aggtgtcgat gccacgctga gccagggccc ttgcccgcga
      241 gaaatcaccg acgaatccga agttccacgc ccccatggcc agggcatctc ccacttccca
      301 tgggttggcg atctgatcca gcaactcgag gcgacgctcg atcacctcgc tgctggctcg
      361 atagtccccg agggagttct ccaccgagcc gactgcgtcg agggcggcgg aggccaggtc
      421 gacccgttgg atgctctctg cgatctcgaa cgcgcgacga cccgccgctc tggtcatgtc
      481 gacgaccgcg gtatcgatat cgcctggctt ccaccgactg aagagaccca tcgaccgagc
      541 gaggaggagt ctgaccatct cctcgctctc gtcacctgga tccaggttgg ccagccccag
      601 ctccaggtag tgattcacct cctcctcgga gaccaaggtc ttcatcgacc ccggccatcg
      661 cgtgggagtc tccaccgcgc gggcgcaggt tctggcgatc aggcgccgct ctccaggggc
      721 gtgctggatc aggaggtccg ctgcctcctt gagggagaac caggcgatat caccggcata
      781 gccggacaga ccggcgagac ctttcgcggt caagccgcgt gcgcgctcca ggggctcgcg
      841 ggccaacaac agcgcctcat cagccagtcg gtgagcttgg tgaacggccg ctcgcccccg
      901 cgacctctcg gaggcggaga ccgtcgcctc gaacgccttg acccggagcg attcgatctc
      961 ctcgctcgtg ctccgagggt cctcttcggc ggcccggaat gcctcgcccc agtggtatgc
     1021 ggtcagctcg accagctcgg agccccgatc cctggtcgcc tcctctatcc aggcgccgac
     1081 cgcggcgtgg gcggcgcccc gctcccggcg ggggaggctg tcataggcca cctcacgggt
     1141 gaggacatgt ttgaagctga actccgcctc gccggcgaga gacgacccga gattcgatct
     1201 gatcagctcc ctgtcctcca gatggtcgag ggtcgagccc aggtcgttgg tgtccgatcc
     1261 gagcagccgg gataccggag tgggccagaa gacccttccc accactgccg ccctctgcag
     1321 gacccgcttc tcgaccgggt cgaggaggtc gatcctggcg gcgagcacgc cctggaccgt
     1381 gtccgggatc tcgacatcgt ccgaatccga cactgcgcgc cagttgctcc cgtcgcggac
     1441 gatgtggccc tggtcgatca gatgacggat gatctcctcc aggaagaagg gattcccttc
     1501 tgccttgttc atcatggcca gtctcaccgt cggggggaga tcggcgatgg agagcaagta
     1561 cccgactagg gcgtcggact cctcgctggt gagaggatcg aggaagatcg acgagtgatt
     1621 cctcttccct ccaccccaac cggggcgtcg gtccgtgagc tcgggtcgcg ccgggcagat
     1681 gatgatgacc gggccggcca ccttctcggt cagctcctcg aggatgtcga gcaacgcggt
     1741 gtcggcccag tggatgtcct cgatgaccgc gaccacggga gattcttcgc tcagggccgt
     1801 gaacagcgag cgccacgccg atcgggtcct cgcccgcacc tctcgtggat cgagctcggc
     1861 gaacgaatga tccgggtctg tgagccccat ggtgtgggcg agggcggcaa cggtcatgcc
     1921 gggatcgtcg gtcagctggg tgaggacact gtctcccagc gcccggatct gctccagggc
     1981 ctccgaggag gtgtcggtgt ccttgatccc ggccacctcc ttcaggatct cggccagagg
     2041 ccagtaggcg acgccgtcac cgtagg
//
LOCUS       DATITW010000332         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_617719, whole genome shotgun sequence.
ACCESSION   DATITW010000332 DATITW010000000
VERSION     DATITW010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617719"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VLS86_04670"
     CDS             complement(<1..1007)
                     /locus_tag="VLS86_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSO49817.1"
                     /translation="MLAAICAVLTAAAAISAFVIQRESTRPIGEGEVFLADAGTVEDF
                     VIEARDPDAAVRRARNELGIEAVSLLGPDAVVTASTSDPLVGSVLHNPLMAEGAHDRR
                     FMGLATAIETDLWLDGVQVWPAGTVLYQVVSPVAGSDRSILLHYDVAQLLGRRAQPGT
                     IQPDAIQLLVLATIFGVFGGAILVGHGRAARRYTEVAAESQLLRTHSEELQAANASLD
                     RARHQAEEALTLAEEKIRIRSEFVLMINHELRTPLTSVVTGAELLRTTRLSEADRAVV
                     LDSMVSDGARLQEIIDQILAVARIENRGMSYELTAVPIAEIRSALASAHQAATAMAVT
                     DL"
     gene            complement(1117..1776)
                     /locus_tag="VLS86_04675"
     CDS             complement(1117..1776)
                     /locus_tag="VLS86_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49818.1"
                     /translation="MTRGSVALVLILIASCYGASTTTTTSTTPTSTTPTSTTATLPGN
                     EVPSIFEAGDVLGVIGVESEPSPRLLSFPGQSGEPVFEVSPLEDDLEALGRAWEIDGV
                     AWEWLLVQGHEGYLPRSWLGFIGEPEDVTSQFAALTSDSVARLGEEIASEIEATKTVL
                     VAQVSPLEVVYDVVGLGDDSIAGYRIRVTAREVTGGFAPESVERSALCTRGLTATGVC
                     L"
     gene            complement(1773..>2965)
                     /locus_tag="VLS86_04680"
     CDS             complement(1773..>2965)
                     /locus_tag="VLS86_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSO49819.1"
                     /translation="LVGHPGRRAAAPAVDGDRVSYTLEEGDRAAVCITALNGAEHLRL
                     VAGDHDFSDLAWLPGDRGLVAIATSRGQSDLVTIGLDGEVSLLAEGGSWQTPVVTSVG
                     VVAVHEAFDASATVVLVAEDGDRSPLYDGTPQAVRAAPHAKLERITFQSSGDLEIEGF
                     LFRPSDTSRPVPAVVYPHGGPTSLYADEWDGHAQYFVDKGYAWLAVNFRGSTSYGLGF
                     ERANHRNWGLGDVDDCLAAANHLAGLGWVDPGRIAIFGASYGAYLALASVVRPENPFA
                     CAVSKYGDCDILTSWAQGDRTGIEDLERMMGHPSENRDGYVAASPIHEIGRITKPILI
                     AHGEHDARVHPRQSQELVDALDRVGATYEYITYPTEGHGLLRREPQLHFYQRLERFLD
                     WYLM"
BASE COUNT          508 a          983 c         1010 g          464 t
ORIGIN      
        1 agatccgtca ccgccatcgc ggtggcggcc tggtgggccg aggccagcgc cgagcggatc
       61 tcggcgatgg gaacggcggt taactcatag gacatccccc ggttctcgat ccgggcgacg
      121 gcgaggatct gatcgatgat ctcctggagg cgggcgccgt cggagaccat cgagtcgaga
      181 accacggccc ggtcggcctc cgagagccgg gtcgtgcgga gcagctctgc gcccgtgacc
      241 acgctggtca ggggcgtcct cagctcgtgg ttgatcatca ggacgaactc ggagcggatc
      301 cggatcttct cctcggccag cgtcagtgct tcctccgcct ggtgacgggc gcggtcgagg
      361 ctggcgttgg cagcttgcag ctcctcggag tgggtgcgaa gcaactgtga ttcggcggcg
      421 acctcggtgt agcgacgggc tgcccttccg tgaccgacga ggattgcccc cccaaacact
      481 ccgaagatgg tggccaggac cagcagctgg atggcgtccg gctgaatggt tcccggctgg
      541 gcccggcggc ccaacaattg agcgacgtcg tagtggagga ggatcgaacg atccgaccct
      601 gccacgggcg acaccacctg atagaggacg gtccccgccg gccacacctg gaccccgtcc
      661 agccagaggt cggtttcgat cgccgtggcc aggcccatga accgtcgatc gtgtgcgccc
      721 tcggccatga gtgggttgtg gagaaccgag ccgacgagtg gatcggaggt cgaagccgtc
      781 accaccgcgt cgggcccgag cagggagacc gcctcgatgc ccaactcgtt gcgcgcccgt
      841 cgcaccgcgg catcggggtc tcgagcctcg ataacgaagt cctccacggt cccggcgtcg
      901 gcaaggaaca cctcgccctc accgatgggg cgggtcgatt cccgctggat tacgaaggct
      961 gagatcgcag ccgcggcggt cagaaccgcg cagatggcgg cgagcaccag ccaccaactc
     1021 gaaggtcgac ggaccagctg cgccacatgc ggtttcacga ggcagaatcg gcagatatca
     1081 ggcttttctt gaggaatgcg gcccgagcga cggggatcag agacagaccc cggtggcggt
     1141 cagaccacgg gtgcagagcg ccgatctctc caccgattcc ggggcgaaac caccggtgac
     1201 ctcccgggcc gtcacccgga tccgatagcc ggcgatcgag tcgtcgccga gcccgacgac
     1261 gtcgtagacc acctcgagtg gcgacacttg cgcaacgagc acggtctttg ttgcttcgat
     1321 ctcgcttgcg atctcctcgc cgagacgggc cacagagtct gaggtcagtg cggcgaactg
     1381 ggaggtcaca tcctcgggct cgccgatgaa cccgagccac gatctcggca ggtatccctc
     1441 gtgcccctgg acgaggagcc actcccaggc aacaccatcg atctcccagg ccctgcccag
     1501 ggcctcgaga tcatcctcga ggggactcac ttcgaatacc ggctccccgc tctgaccggg
     1561 aaacgagagc aaccggggac ttggctccga ttcgaccccg atcaccccca ggacgtcacc
     1621 cgcctcgaag atcgagggga cctcgttccc gggaagggtg gcggtggtgc tggtcggcgt
     1681 ggtgctggtc ggcgtggtgc tggtggtggt ggtcgtgctc gcgccgtagc aggaggcgat
     1741 caggatcagg accagggcca ccgagcccct ggtcacatga ggtaccagtc gaggaacctc
     1801 tccagtcgct ggtagaagtg gagctggggc tcacgtctca ggagcccgtg cccctcggtg
     1861 ggataggtga tgtactcgta ggtcgcccca acccggtcca gggcgtcgac cagctcctgg
     1921 gactgccggg gatggacccg ggcatcgtgc tcaccgtggg cgatcagaat cggcttggtg
     1981 atcctgccga tctcgtggat cggcgaggca gccacatagc cgtcgcggtt ctcggagggg
     2041 tggcccatca tccgctccag gtcttcgatc cccgtccggt cgccctgggc ccaggaggtg
     2101 aggatgtcgc agtcgccata cttcgacacc gcgcatgcga acgggttttc aggtcgcacc
     2161 accgaggcca gagcgaggta ggcgccgtag ctggcgccga atatcgcgat ccgacccgga
     2221 tcgacccagc ccaatccggc gaggtgattc gccgccgcca ggcagtcgtc cacgtctccc
     2281 aggccccagt tccgatgatt ggcccgttcg aacccgagac cgtaggaggt cgagccgcgg
     2341 aagttgaccg cgagccacgc gtaccccttg tcgacgaagt actgggcgtg gccgtcccac
     2401 tcgtcggcgt agagggaggt gggccccccg tgcgggtaga ccaccgccgg aaccgggcga
     2461 gaggtgtccg atggccggaa caggaagccc tcgatctcga gatcgcccga cgactggaac
     2521 gtgatccgct ccagcttggc gtggggggcg gcccgcaccg cctgtggcgt cccgtcgtag
     2581 agagggctcc ggtcaccgtc ctctgccacc aggaccacgg tggcagaagc atcgaatgcc
     2641 tcgtggaccg cgaccacgcc aacagaggtc accaccggtg tctgccacga gccgccttct
     2701 gccagcaggc tcacctcacc gtcgagaccg atggtcacca ggtcgctctg accgcgcgac
     2761 gtggcgatgg cgaccaggcc tcgatccccg ggaagccatg ccaggtcgga gaagtcgtgg
     2821 tcgccggcaa cgagcctgag atgctcggcg ccgttgaggg cggtgatgca cactgcggct
     2881 cgatccccct cttcgagggt gtaggagacc cgatcgccgt cgacggccgg tgccgcggcc
     2941 cgacggccag ggtgcccgac caggg
//
LOCUS       DATITW010000333         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_297207, whole genome shotgun sequence.
ACCESSION   DATITW010000333 DATITW010000000
VERSION     DATITW010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297207"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VLS86_04685"
     CDS             <1..527
                     /locus_tag="VLS86_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49820.1"
                     /translation="EQSGNIATQSITSIRAAAEFFGRDYETEWFSDFKDPLPPMDPDR
                     PLKIAEGPALVLGEWFRFGSEALEELRAIGSEEDDVSEVQLWPEHFDPAIEIGSESQG
                     RRASYGASAGDKGHLEPYLYVAAWSDIDRANPYWNDRHFNGASLPHSELLDADDPKQV
                     ANEFFNWGYQILHR"
     gene            complement(609..1061)
                     /locus_tag="VLS86_04690"
     CDS             complement(609..1061)
                     /locus_tag="VLS86_04690"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HSO49821.1"
                     /translation="MCAGNICRSPAAEAAIVEAAAEAGLDVVVDSAGTGSWHIGQPPH
                     AESVAAGARWGLPIEGRARRVTATDFDRFDIIVAMDRANLRDLIALAPSKEAQAKLRL
                     FRTYDPEATVDEVPDPWGGPAAGYEETVRIVRAAARGLVAEIGEHSRP"
     gene            complement(1073..1978)
                     /locus_tag="VLS86_04695"
     CDS             complement(1073..1978)
                     /locus_tag="VLS86_04695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HSO49822.1"
                     /translation="MATATSRFGSGKRESHDSSAFYDRFAPPLVSPDETVAPAVARDV
                     LFHGDARAITEQQVADRSVALLITSPPYFAGKEYETDLAAGHVPSSYVEYLEMLHDVL
                     SVCLRKLEPGGRMAVNVANLGRKPYRSLSAHIISILQDDLGMLMRGEVIWQKARGAGG
                     SCAWGSYRSPQNPVLRDVTERVVIASKGRFDRALKRSRREADGLPHEATIGTEEFLEA
                     TLDVWEIPSESARKIGHPAPFPVALPQRLIELYTYKGDLVLDPFMGSGSTAVAAARSG
                     RHFVGFDTDETYLALARERLAEEAT"
     gene            2189..>2644
                     /locus_tag="VLS86_04700"
     CDS             2189..>2644
                     /locus_tag="VLS86_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSO49823.1"
                     /translation="MIEEGFNQMSLESRFARFGSGMSTLRAAELRYLTDVDQVSHVAW
                     GATIDNQPAGVGRYILGSGPDAEIAIAVVDRFQRRGLGRVLFDALAASARASGVVGLH
                     FAIEPWNRPVLRMLLGIEIDLNQADDMLEGRIDVADIPTSELAAQFVEVL"
BASE COUNT          457 a          895 c          870 g          422 t
ORIGIN      
        1 acgagcaatc gggcaacatc gccacccaat cgataaccag catccgggca gcagccgagt
       61 tcttcggccg ggactacgag accgagtggt tctccgactt caaggatccc ctgccgccca
      121 tggaccccga ccgaccactc aagatcgccg agggaccggc gctcgtcctt ggcgagtggt
      181 tccggttcgg gtccgaggcg ctcgaggagc tgcgggccat cggcagcgag gaggacgatg
      241 tctccgaagt ccagctgtgg ccggagcatt tcgacccggc gatcgagatc ggcagtgagt
      301 cccagggtcg acgggcgagc tacggggcat cagccggtga caaggggcac ctcgagccgt
      361 atctctatgt cgccgcctgg tccgacatcg accgggccaa cccgtactgg aacgaccggc
      421 acttcaacgg ggcgagtctt ccccactccg agttgctcga cgccgacgac ccgaagcagg
      481 tcgccaacga gttcttcaac tggggctacc agatcctgca ccgctgacct accggccggc
      541 tcgtaattgg gagccacagg tccggatatg acacctgaga ctcccaatta cggcaaagcc
      601 ggcccgggtc aggggcgact gtgctccccg atctcggcga cgaggccacg ggccgcagcc
      661 ctcacgatcc ggaccgtctc ctcgtacccg gcggcgggcc caccccacgg gtcgggcacc
      721 tcgtcgactg ttgcctcggg gtcgtaggtg cggaacagac ggagcttggc ctgggcctcc
      781 ttggacggcg ccagggcgat cagatccctg agattggcgc ggtccatggc cacgatgatg
      841 tcgaaccgat cgaagtcggt ggccgtcacc ctgcgcgccc ggccttcgat gggcagaccc
      901 caacgggcgc cggccgccac cgactcggcg tgtggcggct ggccgatgtg ccaggagccg
      961 gtcccggcgg agtcgaccac gacgtcgagc ccggcctcgg cggccgcctc gacgatcgca
     1021 gcctcggccg ccggagaccg gcagatattg ccggcacaca cggcgaggat cctcaagtcg
     1081 cctcctccgc cagccgctcc ctggccaggg cgagataggt ctcgtcggtg tcgaacccga
     1141 cgaagtgccg ccccgatcgg gctgcggcga ctgcggtcga gccggacccc atgaacggat
     1201 cgaggaccag atctcccttg taggtgtaga gctcgatcag ccgctggggc agcgccaccg
     1261 ggaacggggc gggatgaccg atcttccggg cgctctccga tgggatctcc cacacgtcca
     1321 gcgtcgcctc caggaactcc tcggtgccga tggtggcctc gtggggcaag ccgtcggcct
     1381 ccctgcggct ccgcttcagg gcgcggtcga atcgcccctt ggaggcgatc accacccgct
     1441 cggtgacgtc acgcaggacc gggttctgtg gcgagcggta ggagccccag gcgcagcttc
     1501 cacctgcccc gcgcgccttc tgccagatga cctcgccccg catcaacatc cccaggtcgt
     1561 cctggaggat ggagatgatg tgcgccgaca gcgaacggta cggcttgcgg cccaggttgg
     1621 ccacgttgac cgccatccga ccgcccggct ccagcttgcg cagacacacc gacaagacat
     1681 cgtggagcat ctcgaggtac tcgacgtagg acgagggcac atggcccgcg gcgagatcgg
     1741 tctcgtactc cttgccggcg aagtaggggg gcgaggtgat cagcaacgcc accgagcggt
     1801 cggctacctg ctgctcggtg atggcgcggg cgtcgccatg gaacaagaca tctcgggcga
     1861 ctgctggggc taccgtctcg tcgggggaga ccaggggagg agcgaaacgg tcgtagaaag
     1921 cgctggaatc gtgagattcg cgctttcccg acccaaaccg ggatgtggcc gtcgccattc
     1981 gctgcctcgc catttggaga atgaagttac ccgcggcggt ttggagacac cggtataggg
     2041 cgattgcatg cgctatgctt gctatagcaa gcaccgccga gagcaagaca tcaccaccga
     2101 gaagaccgaa caccttcccc gagtcccagt ggtactccgc gacggcacca ccgccgagct
     2161 gaggccgatc tcgcccgacg accggtcgat gatcgaggag ggcttcaacc agatgtcact
     2221 ggagagccgt tttgcccgct tcgggtccgg gatgtcgact ctgcgagccg ccgagctccg
     2281 ctatctgacc gatgtcgacc aggtcagtca tgtggcatgg ggagcaacga tcgacaatca
     2341 gcctgccggc gtcggccggt acatcctcgg ctcagggccc gacgccgaga tcgccatcgc
     2401 cgtcgtcgac cgttttcagc gacggggtct gggccgagtc ctgttcgacg ccctcgccgc
     2461 ctccgcccgg gccagtggtg tcgtcgggct ccatttcgcg atcgagccct ggaaccgacc
     2521 ggtgcttcgc atgctgctcg gcatcgagat cgacctcaac caggccgacg acatgttgga
     2581 ggggcggatc gacgtcgccg atattccgac cagcgagctg gccgcacagt ttgtcgaggt
     2641 cctg
//
LOCUS       DATITW010000334         4897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_577926, whole genome shotgun sequence.
ACCESSION   DATITW010000334 DATITW010000000
VERSION     DATITW010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577926"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1279)
                     /locus_tag="VLS86_04705"
     CDS             complement(164..1279)
                     /locus_tag="VLS86_04705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSO49824.1"
                     /translation="MTDFVVIGGGIAGVAAAAHLAPHGEVTLLEMEQSLAFHTTGRSA
                     AMFVVNYGGASSRPLALASQAFLESPPDGLVDAPLLSERGALWVADDSQVERMETIAQ
                     EGRDSGAGSLLIDAGEMVALVPVLRPDRIAAGIYEAEARDIDVAALHQGFVRLARRYG
                     AQIQLASPVTGLHRLADGWRVSTTNGAIDCPVVVNAAGAWGDKVAAMAGIEPIGLQPM
                     RRTAFMVPGSDSYATWPLVVDVDHNFYFRPDGSQLLCSLAEETPSEPTDPRPAMEDVA
                     LAIERINQATTLGIHTVSSQWTGLRTFAPDRELVVGEEPTAPGFFWLVGQGGTGIMTS
                     PGYGALVASLVLGSALPGELARAGVDPAALTPDRFRV"
     gene            complement(1276..2247)
                     /locus_tag="VLS86_04710"
     CDS             complement(1276..2247)
                     /locus_tag="VLS86_04710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HSO49825.1"
                     /translation="MRALICERYDGIGALRVGEFPAPEPIPGTAIVNIEAAAVNFADI
                     LMVEGKYQTKPELPFVPGGEFAGIVVTSDGADRLSPGDRVCGFIGIGAMAERAVAYPG
                     SIVKLPEHISYEAGASIPVAYGTSYHALVDRAGLAKNETLLVLGAAGGVGIAAVQIGK
                     ALGARVIGVVSSEVKAEAAREAGADAIVRYDQTPLREGIAGATSGEGVDVVYDPVGGE
                     ATELALRSTKWNGRLLVVGFASGDIPSIPLNLSLVKGNAVVGVFWGRFNIEEPEKSAA
                     NNQVIIDWVDDGTLRPLVQKAYPLDQAVEAFRWVAGRNAVGRVVVTP"
     gene            2297..2974
                     /gene="aat"
                     /locus_tag="VLS86_04715"
     CDS             2297..2974
                     /gene="aat"
                     /locus_tag="VLS86_04715"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HSO49826.1"
                     /translation="MAIEPMPSRFELPDPRAGDGDVVAVGADLEPGTLLQAYRKGLFP
                     MKLPDGQLGWWSPVDRAILPLRGLHISRSLRQSTRRYQVSVDADFEGVIRGCAHPKRG
                     DPWISDEFVNAYVHLHRLGWAHSVEVWDNDGRLVGGLYGVAVGGLFAGESMFHDARDA
                     SKVALVHLVVVMSQGGGRMLDVQWQTPHLESLGAVVIGRDEYLRRLEPALHGPDPFAS
                     AHESDSL"
     gene            3001..3855
                     /locus_tag="VLS86_04720"
     CDS             3001..3855
                     /locus_tag="VLS86_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSO49827.1"
                     /translation="MRAVVAGVDLSAMGRRVAERARMMAESADVPLHLLHVLEPVAEA
                     MIEPSIARLMREFQREEAEKLADWVKERAEVPVDLEVVKGSPSWELTARGKSAGLVVV
                     GSSSIDAFSVGPVAKRVARKSNADTLVVRRQPRVPYRKIIAAVDFSEQSRVAVDRGLS
                     LFPDAEITALYSMPSRFDAILVEAGLFKEELDASRETRLGLAEERMEEYASAWDGSVR
                     TLVVDGPPSQTIEEVVRRRGADLVVVGSRGASATRMVLLGTVAEGLVTQAPCDVLVAR
                     SKAPFRRP"
     gene            complement(3824..>4897)
                     /locus_tag="VLS86_04725"
     CDS             complement(3824..>4897)
                     /locus_tag="VLS86_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSO49828.1"
                     /translation="IPADAFEALIGDSRVVRRMFRTVTSRLRGIEETLRHEERMAALG
                     RMAAQLMHELNNPAAAVSRAMARSQDLYQALGDAAVKLASSGELANREIPSAQKRDDL
                     DPLTRSELEDEMATWLRDLEIDDAWELAPALVADGFSVESLDETTEGLSTETKALFAR
                     WLGLRCLASQILDEARIAVGRISELVRVVKNYSYLDQAPIQEIHPAEGISDTLILLKH
                     KLRGIETVLDIDPDLPNIEASGRDLNQVWTNLLDNAADAMDGQGTLTIRATEEEGQVV
                     IKISDTGGGIDAEVRDRVFDPFFTTKEPGKGTGLGLHTVHTIVTRSGGEIAVDSDGTG
                     TTFTLRFPPASGRLMDAEMAPST"
BASE COUNT          781 a         1599 c         1678 g          839 t
ORIGIN      
        1 tattcggtcg ggagcgaagg gcatggcacg accatctggc cgggactgcg gtggtctacg
       61 actgggggag ccgggccgct cgcatgtcca ccccgctcgc ggcatggctg gagcgcaaag
      121 gctccgacac ctgatcgcgc cggcggtccg gcagcggcaa tagctagacc cgaaagcgat
      181 ccggcgtcaa cgccgccgga tcgactccgg cccgggccaa ctccccgggg agcgcggagc
      241 cgagcacgag gctcgccaca agtgctccgt aaccgggcga ggtcatgatc ccggtgccgc
      301 cctgtcccac cagccagaag aagcccggtg cggtgggctc ttcgcccacg accagctcgc
      361 ggtccggggc aaaggtgcga agacctgtcc actgcgagct gacggtgtga attccgagtg
      421 tggtcgcctg gttgatccgc tcgatcgcca gggccacgtc ctccatggca ggacgggggt
      481 cggtcggctc tgaaggtgtc tcctcggcca gagagcagag aagctgtgac ccgtcgggtc
      541 gaaaatagaa gttgtggtcg acgtcgacca ccaatggcca cgtcgcgtag ctgtcggatc
      601 cggggaccat gaatgcggtt cgccgcatcg gttgcaggcc gatcggctcg atcccggcca
      661 tcgcggccac tttgtcgccc caggcgccgg cggcgttcac aaccaccggg cagtcgatgg
      721 ccccattcgt ggtggagacc cgccagccgt cggcgagtcg gtggagaccg gtgaccggtg
      781 aggccaattg gatctgagct ccgtaacgac gcgccagcct cacgaagccc tggtggaggg
      841 cggcgacatc gatgtccctg gcctccgcct cgtagatccc ggcggcgatc cggtcggggc
      901 gcaacacggg caccagtgcc accatctccc cggcatcgat cagaagcgag ccggctcccg
      961 aatcgcgtcc ctcctgggcg atggtctcca tacgctccac ctgggagtcg tcagccaccc
     1021 agagcgctcc ccgctcggac aacaagggag cgtcgacgag gccatcgggc ggactctcga
     1081 gaaacgcctg gctggcgagt gccagaggcc gggagctggc gccgccgtaa ttgacgacga
     1141 acatcgccgc cgagcggccg gtggtgtgga aggcaagact ctgctccatc tcgagcaggg
     1201 tgacctcgcc gtgtggcgcc agatgggctg cggccgccac tccggcgatg ccacctccga
     1261 tcaccacgaa gtcggtcaag gggtaaccac cacccgtccc accgcgttcc gccctgcaac
     1321 ccaacggaac gcctccactg cctggtcgag ggggtaggcc ttctgcacca gcgggcgcag
     1381 ggtcccgtcg tcgacccagt cgatgatgac ctggttgttg gcggcgctct tctccggctc
     1441 ctcgatgttg aaccttcccc agaacacccc gaccactgcg ttgcccttca ccaggctgag
     1501 attgaggggg atgctcggga tgtcgccaga ggcgaaaccg accaccagga gcctcccgtt
     1561 ccacttcgtg gagcggaggg ccaactcggt ggcttccccg ccgaccgggt cgtagaccac
     1621 atccacacct tcacccgacg tggccccggc tattccctca cggagcggag tctggtcgta
     1681 gcggacgatg gcgtcggcgc ccgcctctct ggctgcttcg gccttcacct ccgaggagac
     1741 gacgccgatc acccgcgccc ccaacgcctt tccgatttgg actgctgcaa tccccacccc
     1801 gcccgcggcg ccgagaacca gcagggtctc gttcttggcc agcccggcac ggtcgacgag
     1861 ggcgtggtag gaggtcccgt aggcgaccgg aatcgaggcg ccggcctcgt acgagatgtg
     1921 ttcgggcagt ttgacgatcg atcccgggta ggcgacggcc cgctcggcca ttgcgccgat
     1981 gccgatgaac ccgcacacac ggtcgccagg ggagagccgg tcggccccgt cgctggtgac
     2041 gacgatcccg gcgaactccc caccggggac gaagggcagc tccggcttgg tctggtactt
     2101 gccttcgacc atcaggatgt cggcgaagtt gaccgcggcc gcctcgatgt tgacgatcgc
     2161 cgtccccggg atcggctcgg gggcggggaa ctcgccaaca cgaagggcgc cgattccgtc
     2221 gtatcgctca caaatcaggg cacgcattgg ggcagcctac ggacaagggc ggtccggctc
     2281 tgacacaatc ggggtcgtgg ccattgagcc gatgccatcg aggttcgaat tgcccgatcc
     2341 acgggccggc gacggggacg tggtggcggt cggagccgat ctcgaaccgg ggaccttgct
     2401 ccaggcctac cgcaagggat tgttcccgat gaagctgccc gatgggcagc tcggctggtg
     2461 gtcgccggtg gatcgagcga tcctccccct gcgcggcctc cacatcagcc gctcgctccg
     2521 gcagtcgacc cggcgctatc aggtgtcggt cgatgcagat ttcgaggggg tcatccgggg
     2581 atgcgcccac cccaagcgcg gcgatccctg gatcagcgac gagttcgtca acgcctatgt
     2641 ccatctgcat cgtctgggat gggcccactc ggtcgaggtc tgggacaacg acggcagact
     2701 ggtaggcggg ctctacggcg tcgccgtggg tgggctgttc gctggagagt cgatgttcca
     2761 tgacgcccgt gacgcctcca aggtggcgct ggttcatctc gtggtggtga tgagccaggg
     2821 cgggggccgg atgctcgacg tgcagtggca gaccccgcat ctggagtcgc tgggggcggt
     2881 ggtgatcggg cgcgacgagt atctcagacg gctggagccg gctctgcacg ggcccgaccc
     2941 cttcgcatcc gcacatgaga gcgacagctt gtagagtcgg ctgcctgtcc tggaggagcg
     3001 ttgcgggccg tcgtcgccgg agtggatcta agcgccatgg gccggagggt ggccgagcgg
     3061 gcccggatga tggccgagtc ggccgacgtc ccgcttcacc tgctgcatgt gctggagccg
     3121 gtcgccgaag cgatgatcga gcccagcatc gcccgtctca tgcgggagtt ccagagggaa
     3181 gaggccgaga agctcgccga ctgggtcaag gagcgggcgg aggtgccggt cgacctcgaa
     3241 gtggtcaaag ggtcgccctc gtgggagttg acggcgcggg gcaagagcgc aggtctggtg
     3301 gtggtcgggt cgtcttcgat cgacgccttc tcggtggggc cggttgccaa gcgcgtcgca
     3361 cgaaagtcga atgcagacac cctggtggtg cgtcgccaac caagggtccc ctaccgcaag
     3421 atcatcgccg ccgtcgactt ctccgagcaa tcgcgggtgg ccgtcgatcg ggggctctcg
     3481 ttgttcccgg acgccgagat caccgcgctg tactccatgc cgtcgagatt cgatgccatc
     3541 ctcgtcgaag cggggctgtt caaagaggag ttggacgctt ctcgtgagac ccggctgggt
     3601 ctggccgagg agcgcatgga ggagtacgcc tccgcttggg acggcagcgt ccgcaccctg
     3661 gtggtcgacg gcccccccag ccagacgatc gaggaggtag tgcggcgaag gggagccgat
     3721 ctcgtcgtgg tggggagccg cggggcgagc gccacccgga tggtcctgtt gggcacggtg
     3781 gccgagggtc tggtgaccca ggcgccatgt gatgtcctgg ttgctaggtc gaaggcgcca
     3841 tttcggcgtc catgagtctt ccgctcgccg gggggaacct gagggtgaaa gtggtgcccg
     3901 taccgtcgga atccacggcg atctcacctc cgctgcgggt cacgatggtg tggacggtgt
     3961 gcaggccgag accggttccc ttgcctggct ccttggtcgt gaagaacggg tcgaataccc
     4021 ggtccctgac ctcggcgtcg atgccacccc cggtgtcgga gatcttgatc accacctgcc
     4081 cctcctcctc ggtggcgcgg atggtcagag ttccctgacc atccatggca tcggccgcgt
     4141 tgtcgaggag gttggtccaa acctggttca ggtcccgccc cgatgcctcg atgttgggca
     4201 gatccggatc tatgtcgagg acggtctcga tccctcgcag cttgtgcttg agcaggatca
     4261 gggtgtcgct gattccttcc gcgggatgga tctcctgaat cggggcctgg tcgagatacg
     4321 agtagttctt caccactcgc accagctcgg agatcctccc caccgcgatc cgggcctcgt
     4381 cgaggatctg tgaggcgaga cagcggagcc cgagccagcg agcgaagagt gccttggtct
     4441 ccgtgctcag gccctcggtc gtctcatcca gcgactcgac gctgaagccg tctgcgacca
     4501 gcgccggcgc cagctcccaa gcatcgtcga tttcgaggtc gcgcagccag gtggccatct
     4561 cgtcctcgag ctccgacctg gtcaacgggt cgaggtcgtc gcgcttttgg gcggaaggga
     4621 tctcgcggtt cgccagctca ccgctgcttg cgagtttcac cgcggcatca cccagggcct
     4681 ggtagaggtc ctgcgacctg gccatcgccc ggctgactgc tgccgccggg ttgttcagct
     4741 catgcatcag ctgggccgcc atgcggccca atgcggccat ccgctcttcg tgtcggagtg
     4801 tctcctcgat gccgcgaaga cgggaggtca ccgtccggaa catcctgcgc accacacgag
     4861 aatcaccgat cagtgcctcg aaggcgtcgg cggggat
//
LOCUS       DATITW010000335         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_497336, whole genome shotgun sequence.
ACCESSION   DATITW010000335 DATITW010000000
VERSION     DATITW010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497336"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..>2198
                     /locus_tag="VLS86_04730"
     CDS             237..>2198
                     /locus_tag="VLS86_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HSO49829.1"
                     /translation="MPSTRGPGFEPVEEPIRAELFSVERLEEHARSLAESQELSSRLG
                     RLRLISRRLADNGEVLLSSYRELSKAIREEALLTPAAEWLVDNFHLVESQLSEIRDNL
                     PPTYYRELPKLDGGHLAGYPRVYGIAWAYVAHLDSRFDPEALRRFVASYQQVEPLTIG
                     ELWAIPITLRLILIENLRRMAESIVLARRFRREAEEIADALLGVGGGQPADTRAALDR
                     YGSTRLPQPFAVQLLQRLRDQDPESTPGLTWLLDRLAAEGSAPDEIVNQTHLRQAATN
                     VTVRNIVTSLRQINYFDWADFVEGVSLVDHVLTAESSYGEMSFATRDRYRHAIEELSK
                     GSGTSELEVARTTLRLAGAQLADRRQRDPGFFLLGEGRTVLEDQIGFRPGAGLALSRF
                     VRRRATTVYVGSVALVSGLVLVWPLLMIESPQWRWLLAFLALIPASDIGVVLVNRAIA
                     ALTHPEELPSLALRHGPTAELRTLVAIPAILSRPDQVDELVERLEVHYLANPDGELHF
                     ALLSDWRDAEEPESADDTELLAAAAQGVARLNKRHGPGLGGGDRFLLLHRHRVWNPGE
                     DRYMGWERKRGKLHELNRLLRGADDTTFIRTDRVPTKPVRYVIALDADSRLPPGAATR
                     LVATIAHPLNRARVDESLGRVVEGYGVLQP"
BASE COUNT          369 a          731 c          726 g          372 t
ORIGIN      
        1 gcccgcatca gcggttcggg aagtgctgtc caccatcgga gtgtccgcgc ctcggacccg
       61 aggaggaatg gggagccctc ccctggggaa gtcaccccac tcccggtcga ccccagctac
      121 gggtaggcac ggatgatgcg agagcctggg gcacctgccg attcggaggc atcccgggcg
      181 gcgacgggtg tctaccgcgt gagagaagca gctcgattct cacgtagtgt gcgatggtgc
      241 catccacccg aggaccggga ttcgagccgg tcgaggagcc cattcgggcc gagctgttca
      301 gtgtcgaacg actagaggag catgcccgaa gtctggccga atcccaggaa ctgagctcgc
      361 ggctcggacg tctgcgactc atatccagac gtctggccga caacggtgag gtgctgctct
      421 cctcgtaccg ggagctgtcg aaggcgattc gggaagaggc cctcctcact cctgcggctg
      481 agtggctggt cgacaacttc catctcgtcg agagccagtt gagtgagatc cgggacaatc
      541 tccctcccac ctactaccgc gagctcccca agctggacgg cggccacctg gccggctacc
      601 caagggtcta cgggatcgcc tgggcgtacg tcgctcatct cgacagtcgg ttcgaccccg
      661 aggccctgcg cagattcgtc gcctcgtacc agcaggtcga accactaacc atcggtgagc
      721 tgtgggcaat cccaatcacg cttcgcctga tcctcatcga gaacctgcga cgcatggccg
      781 agtcgatagt cctggccagg aggtttcgtc gcgaggccga ggagatcgcc gacgcgctcc
      841 tcggggtcgg tggcggtcaa ccggccgata cccgggccgc cctcgaccgc tatgggtcga
      901 cgcggctgcc ccagccgttc gccgtccagc tgctgcagcg tctccgtgac caggacccgg
      961 agagcacgcc gggtctgacc tggctcctcg accgtctcgc cgccgaaggg tccgcaccgg
     1021 acgagatcgt caaccagacc cacctccgcc aggcggccac gaacgtaacc gtccgcaaca
     1081 tcgtcaccag cctccgccag atcaactact tcgactgggc cgacttcgtg gagggggtca
     1141 gcctcgtcga tcacgtcctg accgcggaga gctcctatgg cgagatgagc ttcgccacca
     1201 gggaccgcta ccgacacgcg atagaggagc tgtcgaaggg gtcgggcacc tccgagctcg
     1261 aagtggcccg gaccacgctt cgtctcgccg gcgctcagct cgccgatcgt cgccagcgtg
     1321 accccggctt cttcctgctt ggagagggca ggacggtgct cgaagaccag atcgggttcc
     1381 ggccgggcgc cgggctcgcg ctgtcacgtt tcgtgagacg acgtgcgaca accgtgtacg
     1441 tgggctcggt ggcgttggtg tcaggtctcg tcctggtttg gcccctgctc atgatcgaga
     1501 gcccgcagtg gcgatggctc ctggcgtttc tggccctgat ccccgcctcg gacatcgggg
     1561 tggtgctggt caaccgcgcg atcgctgctc tgacccatcc cgaggaactg ccgtcgctgg
     1621 ctttgcgtca tgggccgacc gcggagctgc gcacgctggt ggcgatcccc gccatcctgt
     1681 cccggcccga tcaggtcgac gagttggtgg agagactcga ggtccactac ctggccaacc
     1741 ccgacggcga gctccacttc gccctgctca gcgactggcg ggacgccgag gagcccgaat
     1801 cggccgacga caccgaactg ctggcggcgg cggcccaggg agtggcccga ctcaacaaga
     1861 gacatgggcc gggattgggc ggtggagatc ggttcctgct cctgcatcgg caccgggtgt
     1921 ggaacccggg ggaggaccgg tacatgggat gggagcgaaa gcggggcaag ctccacgagc
     1981 tcaaccggct attgcgtggt gcagacgaca ccacgttcat tcgcaccgac cgggtgccga
     2041 ccaaaccggt tcgatatgtg atcgctctcg acgccgactc tcgtttgccg ccgggggcgg
     2101 ccacccgact tgtggcgacg attgcccatc cactcaacag ggcccgcgtc gatgaatctc
     2161 tcgggagggt ggtggaggga tatggagtct tgcagccg
//
LOCUS       DATITW010000336         1726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_377330, whole genome shotgun sequence.
ACCESSION   DATITW010000336 DATITW010000000
VERSION     DATITW010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377330"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..>1726
                     /locus_tag="VLS86_04735"
     CDS             74..>1726
                     /locus_tag="VLS86_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49830.1"
                     /translation="MTSPRAANREIPWPIFLTLAGISLVIFVVMNPWLIISATTPTGG
                     DMGAHVLGPAFLRDSLLSEGRVMGWSNDWFAGFPAFYFYFPLPSLTIVFLDLFLPYGV
                     AFKVVTVMGLLATPPSAYFFARSMLLGKHVSLIAAGSGVVFAFFESYSIYGGNVASSL
                     AGEFAYSWSFALSFIYLGLLIRAVRDDRKYAKWAALALAATALSHILTTLVVVFASLF
                     VLPWRKGFWRTLAIWAWGFSIAAFWALPLMARIGLTSDMSWTPLSRWEEIFPVELWLL
                     VPLAIPGAVWLSRRTSRAAPLLAATLLPIIYFPLPNIMPTLFPSVFGGERWKLWNGRL
                     LPYWYFGIAFLAAIAVGAAVVWLSRRLPSRLSAHWPRALLVLITIVATALVADSADFP
                     VWAWVPVAVAGAILVGVSFLWVTPVSTRNFLTGSAVVVVVLGALSGVTFIDGWSKWNY
                     EGYESKARWPEYEALMAEINALPDGRVHWEANSELNEFGTPMSPMLIPYWTEGTHPSM
                     EGLYFESSLTTPFHFINSSEMSERPSNPIPGLQYHTGEMERGL"
BASE COUNT          237 a          583 c          513 g          393 t
ORIGIN      
        1 agagatggtg gaatcgtgat ggtgtcgaga tcgacatcac ccaggttcta gagatcgtcc
       61 gacagcaccg ggcgtgactt caccccgggc ggcaaaccgg gaaatcccct ggccgatctt
      121 cctcaccctc gccgggatct ccctcgtcat cttcgtggtg atgaacccct ggctgatcat
      181 ctcagccacc acgcccaccg gtggtgacat gggggcccac gtcctgggcc cggcgttcct
      241 gcgtgacagt ctcctctccg agggcagggt catgggttgg tccaacgact ggttcgccgg
      301 gtttcccgcc ttctacttct acttcccact tccctcgctg accatcgtct ttctcgatct
      361 gttccttccc tacggggtcg cgttcaaggt ggtcacggtc atgggtctgc tggccactcc
      421 cccctccgcc tatttcttcg cgcgctcgat gcttctcggc aagcacgtct cgctcatcgc
      481 agccggatcc ggagtcgtct tcgccttctt cgagagctat tcgatctacg ggggcaatgt
      541 cgcctccagc ctggccgggg agttcgcata ctcgtggtcg ttcgccctgt cgttcatcta
      601 tctcggtctt ctgatacgtg ccgtgcgtga cgaccgcaag tacgccaaat gggctgcttt
      661 ggccctggcc gcgactgctc tgtctcacat cctgaccact ctcgtcgtgg ttttcgcctc
      721 cctgttcgtc ctgccgtggc gaaagggctt ttggcggacc ctcgccatct gggcctgggg
      781 attctccata gccgccttct gggcgcttcc actcatggcc cgaatcgggc tcacctctga
      841 catgtcgtgg accccgctct cgcggtggga ggagatcttc ccggtcgagt tgtggctgtt
      901 ggtcccgctc gccattcccg gtgcggtctg gctcagccgt cgcacatcga gggccgcccc
      961 gctcctggcc gcgactctgc tcccgatcat ctacttcccg ctccccaaca tcatgcccac
     1021 cctcttccca agcgtgttcg ggggggagcg ctggaagctg tggaacggca gactgcttcc
     1081 ctactggtac ttcgggattg cgttcctggc cgcgatcgcc gtgggcgccg ccgtggtgtg
     1141 gctgtcgcgg agattgccgt cccggttgtc cgctcactgg ccgagggcgc tgctcgtcct
     1201 gatcaccatc gtcgccaccg ccctggtcgc tgactccgcc gacttcccgg tctgggcctg
     1261 ggtgccggtg gcggttgccg gagcaatcct ggtcggagtc tccttcttgt gggtcacccc
     1321 cgtctccacc cgcaacttcc tcaccgggag cgcggtggtc gtcgtggttc tgggcgctct
     1381 ttccggggtc acgttcatcg acggctggtc gaagtggaac tacgagggct acgagtcgaa
     1441 agccaggtgg cccgagtacg aggcgttgat ggccgagatc aatgcattgc ccgacgggag
     1501 ggtgcactgg gaggccaaca gcgagctcaa cgagttcggg accccgatgt cgccgatgct
     1561 catcccgtac tggacagagg gcacgcatcc ctcgatggaa gggctctact tcgagtcctc
     1621 gctgaccacg ccgtttcatt tcatcaactc ctccgagatg tcggagcgtc cgtcgaaccc
     1681 gataccgggg ttgcagtacc acacgggtga gatggaacgc ggcctc
//
LOCUS       DATITW010000337         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_17131, whole genome shotgun sequence.
ACCESSION   DATITW010000337 DATITW010000000
VERSION     DATITW010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17131"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VLS86_04740"
     CDS             complement(<1..200)
                     /locus_tag="VLS86_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49831.1"
                     /translation="MTAIDREQRITPEVGAPRRSVVLLTAGILALVVGFGSMAGGIFG
                     AWYTWDQAVAQDVVTPDDAVIA"
     gene            353..1150
                     /locus_tag="VLS86_04745"
     CDS             353..1150
                     /locus_tag="VLS86_04745"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSO49832.1"
                     /translation="MRLSDRVVVVTGASGIAAAGARRFGAEGAAVFVTAIDEGQCRGL
                     AEELGQSGVTVDWSRADLTDEAEATSAFSDCLQRFGRIDGLFAVAGVSGRGFGDGPLH
                     AIPMGGWTRTIEANTVPPFLAARESIKAMRSNSPSPGGTRGSVVLVGSVLSQHPSRLF
                     ATHAYSAAKGATTSMARAMASYYAPEGIRVNVIAPGLVRTPMSERAAQDAESVAYAAA
                     KQPLAGGFLDPEDVAFAASFLLSDEASRITGQVLEVDGGWGVTEVSP"
     gene            1147..1761
                     /locus_tag="VLS86_04750"
     CDS             1147..1761
                     /locus_tag="VLS86_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HSO49833.1"
                     /translation="MTHAELPESILKQRVIAVARGLTSDSAPILAAALQRGGLTVLEV
                     TVEGVGGIDAISSLSGSDVTVGAGTITSVALAAAAVEAGASFLVSPHFDAALTGWALE
                     RGVPMVPGGFTPTELHAAWEAGAPAVKVFPASVGGPGLIRAIKAPFPEMRLIPTGGIT
                     ADNAAAYLQSGASAVGVGEWLTGSPDPGVVTDRAVLLVDSLQLV"
     gene            1794..>2268
                     /gene="deoC"
                     /locus_tag="VLS86_04755"
     CDS             1794..>2268
                     /gene="deoC"
                     /locus_tag="VLS86_04755"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HSO49834.1"
                     /translation="MQPTSLTVDDVSYQQLAGTIDHSLLKPQLTLDDVVEGCALAARY
                     EVASVCVRPSDVALAGAELIDTSVAVGTVVSVPHGDSLTTTKAFESVKAIEDGAVELD
                     MVLNIGLLLSGELSRAEDDIRAVVEVAAGIAIVKVILENAYLTDEQKVQACRLAES"
BASE COUNT          351 a          736 c          793 g          388 t
ORIGIN      
        1 tcggcgatga cggcgtcgtc cggggtcacc acgtcctggg ccactgcctg gtcccaggtg
       61 taccaggcgc cgaagatgcc gccggccatc gaaccgaacc cgacgaccag cgccaggatc
      121 ccggcggtga gtagcacgac ggacctacga ggggcgccga cctctggggt gatccgctgc
      181 tccctgtcga ttgcagtcat ttgttgcctc cttgttccta cgggctaact gctgtatccg
      241 gtcatcctcg gcgagagggc agggaggcgg tgaggggatt aaggccccag ctgcgggggc
      301 cggaagacca cagctgaacg ggaccggccc ggctctgcta caaactcgac ggatgcggct
      361 ctccgatcgg gtggtggtgg tgacaggtgc atcggggatc gctgcggccg gagcccgcag
      421 gtttggtgcg gagggcgccg cggtcttcgt gaccgccatc gacgaaggcc agtgtcgagg
      481 tctggccgag gagctcggcc aatccggcgt gaccgtcgat tggagccgcg ccgacctcac
      541 cgacgaggcc gaggccacgt cggcgttctc cgactgtctg cagcggttcg ggcggatcga
      601 tggcttgttc gcggtggccg gcgtgagcgg caggggcttt ggagacggcc cgctccatgc
      661 catacccatg gggggatgga cgagaacgat cgaggccaac acggtcccgc cctttctcgc
      721 cgcacgggag tcgatcaagg cgatgaggtc caactccccg tcgcccggtg gcaccagggg
      781 ctcggtggtc ctggtcggca gcgtcttgtc acagcacccg tcgcggctgt tcgccacgca
      841 cgcctattcc gcggcaaagg gggcgacgac ctcgatggcc agggccatgg cctcctacta
      901 cgcacccgag gggattcggg tcaatgtcat cgcgcccggc ctggtccgga ccccgatgtc
      961 ggagcgcgcc gcgcaggatg cggagtcggt ggcttacgcc gcggccaagc agcccctggc
     1021 cggcggtttt ctcgatcccg aagacgttgc cttcgccgcc tcgttcctgc tctccgacga
     1081 agcgagccgc ataacgggcc aggtgctcga ggtcgacggg ggatggggtg tcaccgaggt
     1141 gagcccatga cacatgctga attgcccgag tccatcctga agcagagagt catagccgtg
     1201 gcccgtggat tgacctccga ttctgcgccg atcctcgctg ccgcgctgca gcgaggaggc
     1261 ctgacggtcc tcgaggtgac cgtcgagggt gtcggcggca tcgatgcgat ctccagtctg
     1321 agcggctcgg atgtcacggt cggggcgggg accatcacct cggtcgccct ggccgcagcg
     1381 gccgtcgagg cgggggcgag cttcctggtc tcgccgcatt tcgatgccgc gctcaccgga
     1441 tgggcgctgg agcgaggtgt ccccatggtc cccggggggt tcacgccgac ggagttgcac
     1501 gcggcctggg aagcgggtgc gcctgccgtc aaggtcttcc cggcgtcggt tggcggcccg
     1561 ggtctgatcc gggcgatcaa ggcaccattc cccgagatgc ggctgatccc gaccggaggg
     1621 atcacagccg acaacgcggc ggcctatctc cagtccgggg ccagcgccgt cggtgtcggc
     1681 gagtggctga ccgggagccc cgaccccggt gtggtgaccg accgggcagt ccttctggtc
     1741 gactccctcc aacttgtcta gacatatgtc cgcctcccta ccagactcgg atcatgcagc
     1801 ccacctcctt gacggtcgac gacgtcagct atcagcagct ggcagggacg atcgaccatt
     1861 cactcctcaa gccgcaactg accctcgatg acgtggtcga aggctgtgcc cttgccgcca
     1921 gatacgaagt cgcgtcggtc tgtgttcgtc cttcggatgt cgcccttgcc ggcgccgagc
     1981 tgatcgacac ctccgtggcg gttggaaccg tggtcagcgt cccccacggc gacagcctga
     2041 cgacgaccaa ggccttcgag tcggtgaagg ccatcgagga cggcgccgtc gagctggaca
     2101 tggtgctcaa catcgggctg ctgctctcgg gcgagctgag tcgggccgag gacgacatcc
     2161 gcgctgtcgt agaagtcgcg gccggcatcg caatcgtgaa ggtcatcctg gagaacgcgt
     2221 atctgaccga tgagcagaag gtgcaggcct gtcggctggc ggaatcgg
//
LOCUS       DATITW010000338         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_297355, whole genome shotgun sequence.
ACCESSION   DATITW010000338 DATITW010000000
VERSION     DATITW010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297355"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..342)
                     /locus_tag="VLS86_04760"
     CDS             complement(25..342)
                     /locus_tag="VLS86_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007500458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49835.1"
                     /translation="MHRVELAAGDWSIPACLASSFADRFFGNRRAPRGSAVVLRTRSV
                     HSFGQREPIEVVGLDSEMRVVAIRTLRPNRIALILAARTIVELPRGAGLPAVHDRIEI
                     RRG"
     gene            complement(385..1695)
                     /locus_tag="VLS86_04765"
     CDS             complement(385..1695)
                     /locus_tag="VLS86_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49836.1"
                     /translation="MIRRVVYLLALALLVAACTATSGETTTTVAGETTTTRAATTTTA
                     EGSTTTTLPEPTWEELPGIDGLPQEVQDELLGLVRVTEEIRGLRFIDPPIISLVTDEE
                     LATRVRAKIEEEAEDFPADEALYKLLGLIDDEVDLTSLLSDLYGEQVAGFYDGKTGEL
                     VVPIREDGFTVVQRSTLVHELVHSLTDQQFEFFPALQGMLDEERLDQASAYQALIEGD
                     ASMADLLYLQTLTQREIGEFFAEALSIDTTALDSAPAFIRDSLIFPYDSGLAWVQEQY
                     LRDEWATVNAAYSEMPDLPGSTEQIITPDDYGRDLPQTVESALPPVPGYELERESVWG
                     EFGFRLMLDQVLGEDVGVDAADGWGGDYYAQWFDGQNAALLILFEGDTERDTEELRSA
                     LLDYATTAVAEEDWVWVEVLDGRLAFIAADDTGVGESILASIGG"
     gene            1862..>3112
                     /locus_tag="VLS86_04770"
     CDS             1862..>3112
                     /locus_tag="VLS86_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2096..2098,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSO49837.1"
                     /translation="MKRNYARLTEPLVRDNGALRPATMTEAVERVASGLAGHRGESFG
                     LLSCSKGTNEMNYMAQKFSRVVMASNNIDSCNRTUHAPSVAGLAAVFGAGGGTSSYRE
                     IEETDLIVMWGSNAREAHPIFFHHVLKGIHNGARMYAVDPRRTSSAQWADLWLGIDVG
                     SDIALSNALAREIIHSDLHHKEFIDHATEGFDAYAASVEPYTLQRAQEITGVPAYAIR
                     TLAHEYALAGTAQLCWTLGITEHHNATDNVLALINLALLTGHVGRYGSGLVPLRGQNN
                     VQGGGDMGALPNKLPGFQDVENPELRSRFETAWGASIPAKAGMHISLMHEAMDEGRLN
                     ALYVIGENPLQSEADGRLMERRLRGLDFLVVQDLFLTATAEIADVVLPAAGWGETEGT
                     FTSSERRVQRVRRAIAPPGESRDDI"
BASE COUNT          567 a         1029 c          995 g          521 t
ORIGIN      
        1 gcacaaccca ggagcgatcc gccctcagcc acgtcggatc tcgatcctat cgtggaccgc
       61 gggcaggccg gcgcctcggg gaagctccac gatcgtcctc gcggccagga tcagcgcgat
      121 ccgattcggg cgcagggtcc tgatcgcaac caccctcatc tcggagtcga gcccgacgac
      181 ctcgatcggt tccctctgac cgaagctgtg cacgctccgt gtccgcagca cgaccgccga
      241 tccgcgagga gcccgcctgt tgccgaagaa tcgatcggcg aagctggagg cgagacaagc
      301 ggggatcgac cagtcacccg ctgccagctc gacccgatgc atgtggtcga cgctaggaga
      361 tccgggtggc ggggggaagc ccgctcagcc cccgatcgag gccaggatcg attcgccgac
      421 cccggtgtcg tcggcggcga taaaggcgag acgtccgtcg agcacctcga cccacaccca
      481 atcctcctcg gccaccgcgg tcgtggcgta gtcgagcagc gcggagcgaa gctcttcggt
      541 gtcgcgctcg gtgtccccct cgaagaggat gaggagggca gcgttttgac cgtcgaacca
      601 ttgcgcgtag tagtcaccac cccatccgtc ggcagcgtcg acaccgacat cctcccccag
      661 gacctgatcg agcatgagcc ggaagccgaa ttcaccccac accgactccc gctccaactc
      721 gtagccggga accggaggaa gggcggattc gaccgtctga ggcagatcac gcccgtagtc
      781 gtcaggagtg atgatctgct cggtcgatcc gggaaggtcc ggcatctcgg agtatgcggc
      841 attcaccgtc gcccattcgt ctcgcaggta ctgctcctgc acccaggcca ggccagagtc
      901 gtaggggaat atcagcgaat cccggatgaa ggcgggggca gagtccaggg cggtggtgtc
      961 gatgctcagc gcctcggcga agaactcgcc gatctcacgc tgggtcagag tctgcaggta
     1021 gagcaaatcg gccatgctgg cgtcgccttc gatcagggcc tggtatgccg aggcctggtc
     1081 gagacgctct tcgtccagca tcccttgcag agcggggaaa aactcgaatt gctggtcggt
     1141 gagggaatga accaactcgt gaaccagcgt gctcctctgc accaccgtga acccgtcctc
     1201 gcggatcggg accaccaact cgccggtctt gccgtcatag aagcccgcga cctgctcgcc
     1261 gtagagatcc gagagcagtg acgtcaggtc gacttcgtca tcgatcaacc cgagcagctt
     1321 gtacagggcc tcgtcggccg ggaagtcctc ggcttcttct tcgatcttgg ccctcacccg
     1381 ggtggcgagc tcttcatcgg tgacgaggga gatgatgggc gggtcgatga acctcaggcc
     1441 acgaatctcc tcggtgaccc gcaccaggcc caacaactcg tcctggacct cctgcggcag
     1501 accgtcgatc ccgggcaatt cttcccatgt cggctcgggg agggtcgtgg tggtgcttcc
     1561 ctcggccgtg gtggtcgtgg ccgctctcgt ggtcgtggtc tcgccggcca cggtggtggt
     1621 ggtctctccg gacgtggcgg tgcaggccgc caccaggagg gcgagcgcga ggagatagac
     1681 gacacgtcgg atcatggcgg tcaggttatc ggcagtccgg cgctttgagt tgacgcgccc
     1741 ctcaccacaa tcccgcggtc ccttccccgt aattgggagc cccaggtccg catatgaccc
     1801 ctgtgcgtcc caattacgga ggggcggggt acgctcgccg acatcgagga aaggaggtga
     1861 attgaagaga aactatgcac ggctcaccga gcccctggtc cgggacaacg gagcgttgcg
     1921 gcccgcaacc atgaccgagg ccgttgagcg ggtggcctcc ggtctcgccg gacatcgggg
     1981 cgagtccttc gggctgctca gctgttcgaa ggggaccaac gagatgaact acatggcaca
     2041 gaagttctcc cgggtggtca tggcgagcaa caacatcgac agctgcaatc gcacttgaca
     2101 cgctccatcc gtcgccggtc tggcggcagt gttcggggcc ggtggcggca ctagctctta
     2161 tcgagagatc gaggagacag acctcatagt catgtgggga tcgaacgcgc gcgaggcgca
     2221 tcccatcttc tttcatcacg tcctcaaagg gatccacaac ggcgcccgga tgtacgccgt
     2281 cgacccaaga cggacctcgt ctgcgcagtg ggccgacctg tggctgggga tcgacgtcgg
     2341 ctccgacatc gccctgtcca atgccctggc cagggagatc atccattccg acctccacca
     2401 caaggagttc atcgatcacg ccaccgaagg cttcgatgcc tacgccgcct cggtcgagcc
     2461 ctacacgctg cagcgggcgc aggagataac cggtgtcccc gcctacgcca tccgcaccct
     2521 ggcccacgaa tacgccctgg ccgggaccgc tcaactgtgc tggaccctgg gcataaccga
     2581 gcaccacaac gcaacggaca acgtcctcgc tctgatcaat ctcgccctgc tcaccgggca
     2641 tgtcggtcgc tacgggtcgg gtctggtccc gctgcggggc cagaacaacg tccagggcgg
     2701 gggcgacatg ggcgctctgc ccaacaagct ccccgggttc caggacgttg agaaccccga
     2761 gcttcgctcc cggttcgaga cggcgtgggg ggcatcgatc ccggccaagg caggtatgca
     2821 catcagcctc atgcacgaag cgatggacga ggggcgtctc aacgctctct acgtcatcgg
     2881 ggagaatccc ctccaatccg aggccgacgg aaggctgatg gagcgccgtc tccggggtct
     2941 cgacttcctt gtggtccagg acctgtttct gaccgccaca gccgagatcg ccgacgtggt
     3001 cctgcccgcc gccggatggg gcgagaccga agggaccttc acctccagcg agaggagggt
     3061 gcagcgggtg cggcgggcga tcgcgccccc cggggagtcc cgcgatgaca tc
//
LOCUS       DATITW010000339         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_657681, whole genome shotgun sequence.
ACCESSION   DATITW010000339 DATITW010000000
VERSION     DATITW010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657681"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VLS86_04775"
     CDS             complement(<1..702)
                     /locus_tag="VLS86_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HSO49838.1"
                     /translation="MDLVFRALADPTRRSLLDELLHNDGQTLSALEERFPMTRFGVMK
                     HLKLLEEAGLVVTKRRGREKLHYLNPVPIRLVHDRWVSKYAEPWASALSELKQRLETP
                     MEKVFEIYIRTTPERLWEAITDSEIRSKYQFGNRIVSDWSPGSRFELTNPNADGPLGE
                     GENLEVDPPRRLVQSMVALWGEDVKSEGTSRITWEIEPVGDSCRLTVTHDQLREDAND
                     QLYGGWPMILSGLKTW"
     gene            complement(819..1349)
                     /locus_tag="VLS86_04780"
     CDS             complement(819..1349)
                     /locus_tag="VLS86_04780"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HSO49839.1"
                     /translation="MDEVQLSTTEYAVLGLLEEGPTHGFALAKLLGPEGAVGRILTVR
                     RPLIYRALDRLVEVGFAEPIHREPGDSGPQRTIHKITSAGRRRINRWLKQPVSHIRQM
                     RIEFQLKLAILHRLGRSPFDLIVAQRDALRPTLTALDDGGGDTTDHLELWRQHNAAAA
                     AAYLDHLIRLSSEHSS"
     gene            1426..2190
                     /gene="modA"
                     /locus_tag="VLS86_04785"
     CDS             1426..2190
                     /gene="modA"
                     /locus_tag="VLS86_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014987325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HSO49840.1"
                     /translation="MSLSRRMMAAGTLTVVVIGCAASDDPGRGLEGEVLVSAAASLSD
                     VFAEVEAAFETATPGIDVLVNLGSSSSLREQILEGAPADVFASANSSNMDQLVAAGEV
                     SGDPKIFARNLLQIAVPSGNPGHVTGLEDFADEELLIGLCAEDVPCGDFARVALKNAG
                     VIPAIDTNELDVRALLTKIEAGELDAGITYVTDVLSTGGAVEGIDIPEELNVVADYPI
                     ASLAHAPNPDAAAAFVAFVLSDEAQDILDNYGFSSP"
     gene            2187..>2629
                     /locus_tag="VLS86_04790"
     CDS             2187..>2629
                     /locus_tag="VLS86_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018532741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HSO49841.1"
                     /translation="MTTDRPERRRRRPRPPTLLVILGGLGLAVFVVPLIGLLSRTPWS
                     ELPALLGEEVVTDALRLSLVTSVAATAIAAVVGVPLAWLMARVDVKGRGLVRALVTLP
                     LVLPPVVGGAALLFALGRRGIIGEPLSEATGLLLPFSIWGVVAANT"
BASE COUNT          413 a          824 c          863 g          529 t
ORIGIN      
        1 ccaggtcttg agcccggaga ggatcatcgg ccatccgccg tagagctggt cgttggcgtc
       61 ttcacgcagt tggtcgtggg tgactgtgag gcgacaggag tcaccgaccg gctcgatctc
      121 ccaggtgatc ctcgaggtgc cttcgctctt cacgtcttca ccccagagcg caaccatgct
      181 ctgcaccaag cgccgcggcg ggtccacctc gaggttctcc ccttcgccga gaggcccgtc
      241 cgcgttcggg ttggtcagct cgaaccgtga accgggggac caatccgaga cgatccgatt
      301 cccgaattgg tacttgctcc tgatctcgct gtccgtgatc gcctcccaca gtcgctccgg
      361 cgtggtgcgt atgtagatct cgaatacctt ctccatgggt gtctccaatc tctgtttgag
      421 ctcgctgagc gctgaggccc agggctcggc gtacttgctc acccatcggt cgtggacgag
      481 ccggatcggg accgggttca ggtagtgcaa cttctcacgg ccgcgacgct tggtcacgac
      541 cagaccggcc tcctcgagca gcttgaggtg cttcatgacc ccgaatcggg tcatcgggaa
      601 gcgttcctcg agtgcgctca gtgtctgccc gtcgttgtgg agcaactcgt cgagaaggct
      661 ccgtcgtgtt gggtccgcca gcgctctgaa caccaggtcc atgggcggga ctatacgtga
      721 ccaaatggtc acatgtcaac accctgatgc cgagactgtc gaccgcgctg cgaagccgtt
      781 cgacttccgc cgacccggcg cgtcgaggtt ccactggatc aagacgagtg ctcagaagag
      841 agtcggatga gatgatccag ataagcggcc gcggcggcgg cgttgtgttg tctccacaac
      901 tcgagatggt cggtggtgtc tcctccaccg tcgtcgaggg cggtgagcgt cggccgcagg
      961 gcgtcacgtt gggcgacgat cagatcgaag ggtgacctgc ccaggcgatg gaggatcgcc
     1021 agcttcagct ggaactcgat tcgcatctgt cgtatgtggc taaccggctg tttcagccaa
     1081 cggttgatgc gtcttctgcc cgctgaagtg atcttgtgga tggtgcgttg tgggcccgag
     1141 tcaccaggtt ctcggtggat gggctcggcg aatccgacct cgaccaaccg gtcgagggcg
     1201 cggtagatga ggggacgtct cacggtcagg atccgcccca cggcaccctc cggcccgagc
     1261 agcttcgcca gggcaaaccc gtgggtcggg ccttcttcga gcaggcctag caccgcgtac
     1321 tcggtggtcg acagctggac ctcgtccatc gatcgatgag agtaccgctc cgaaagccga
     1381 gctagtctca ttgtgactaa tgaggacgga tatccgagga gtgtgatgtc tctcagcaga
     1441 cggatgatgg ccgccgggac gctgacggtt gttgtgatcg gctgtgccgc atcggatgac
     1501 ccgggccgcg gtctcgaagg cgaggtgttg gtgtccgccg cggcgtccct gagcgacgtc
     1561 ttcgccgaag tcgaggcggc gttcgagacc gccaccccgg ggatcgatgt cctcgtcaat
     1621 ctgggcagca gctcatcgct gcgggagcag atcctcgaag gtgcgccggc ggacgtgttc
     1681 gcgtcggcca actcctccaa catggatcag ctggtagctg ccggggaggt gtccggcgat
     1741 ccaaagatct tcgcccggaa cctactgcaa atcgccgtcc cttccggcaa ccccgggcac
     1801 gtcaccgggc tcgaggactt cgccgacgag gagttgttga tcgggctctg tgccgaagac
     1861 gttccttgcg gcgattttgc ccgggtcgcg ttgaagaacg caggggtcat cccggcgatc
     1921 gacaccaatg agctcgacgt gcgcgccctt ctcaccaaga tcgaggctgg ggagttggac
     1981 gcggggatca cgtatgtgac cgatgtcctg tccaccgggg gagccgttga gggcatcgac
     2041 atccctgagg agctcaacgt ggttgcggac tacccaatcg cctccctggc ccacgccccc
     2101 aatccggatg cagcggccgc gttcgtagcg ttcgtcctgt ctgacgaggc tcaggacatt
     2161 ctcgacaact acggtttctc ctcaccttga ccaccgacag gcccgagcgg cgccgccgta
     2221 gaccgcggcc tccgaccctc ctcgtcatcc tcggcggcct cgggctggcg gtgttcgtgg
     2281 tcccgctgat cgggttgttg tccaggacgc catggtcgga gttgcccgct ctcctcggcg
     2341 aagaggtggt gaccgacgcc cttcggctgt ccctggtcac ctcggttgcc gctaccgcga
     2401 tcgcagcagt ggtcggcgtg ccgcttgcct ggctgatggc ccgggtcgac gtcaagggga
     2461 ggggcttggt gcgagcgctg gtcactctgc cgctggtgct ccctccggtg gtgggaggcg
     2521 ccgctctcct gttcgcgttg gggcgacgag gcatcatcgg ggaaccgctc agtgaggcga
     2581 ccggattgct tctcccgttc tcgatctggg gcgtggtggc cgccaacac
//
LOCUS       DATITW010000340         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_97562, whole genome shotgun sequence.
ACCESSION   DATITW010000340 DATITW010000000
VERSION     DATITW010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97562"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VLS86_04795"
     CDS             complement(<1..1015)
                     /locus_tag="VLS86_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSO49842.1"
                     /translation="MRGRPLLYTSYEADQALLNTTAKRVMLVVFFLVLVSIPFQVIPG
                     LGFLGEDDWLRLLSTTAIFAVGALGLNILTGLAGQVSLGHSFFMGIGAYTSVWLGGAV
                     GGPLIGLGLPIWIWLPAAGIVAALVGVAVGPTAVRVRGLYLAIVTLGLVFIGEYFFRN
                     WNSLTGGAQVGRDFPPLQLRIWKEEPPLIDFTTTSTWLGVEVSGTAKSFFFLVLLAVV
                     FTLLAKILQRTRVGRAFMSIRDRDIAAEIMGVNEFAYKLLAFGISSFFAGIAGALLAS
                     YVSRVIPERWDLFLSVQFIAIVLIGGAGTVTGAILGTAFVVLLPRVVQNFTLWMQSVA
                     VEGD"
     gene            complement(1020..1994)
                     /locus_tag="VLS86_04800"
     CDS             complement(1020..1994)
                     /locus_tag="VLS86_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSO49843.1"
                     /translation="MTPSFVVLAQETAVPNEFAQMLVDGISFGAIYALLAMGFVIIFK
                     ATSVLNFAHGALTGLGAFLVAAFATVIDFPGRYLTFLPEWAQWTANVVTALLVAALFG
                     VILERIFLRPMVGEALFAVAIVTLGIDIVVRTITNDFIGTQPRPIGAPWGLNGIDLGW
                     VRISYSEVIQIVVTVALFAAVALFFRSRTGIAMRATAFDQETARAQGINVGRIFSVAW
                     AIGAFLAGVAGLFFSVYPRRATGVDQATAFIAFRAFPAVIIGGLDSIVGAVVGGFTVG
                     IAEAAANVYLGFEFLGSGFPGIVGYLLMMVVLLIRPYGLFGTPEIRRV"
     gene            complement(1991..>2193)
                     /locus_tag="VLS86_04805"
     CDS             complement(1991..>2193)
                     /locus_tag="VLS86_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HSO49844.1"
                     /translation="PEVIGLIQSVVSEANAAHSHLEAIQKFRLIPKELDHEDGELTAT
                     QKLKRSSMSDMFGDLIEDMYRS"
BASE COUNT          468 a          702 c          711 g          312 t
ORIGIN      
        1 cgtcgccctc cacagccacc gattgcatcc acagggtgaa gttctggacg acgcggggca
       61 acaagaccac gaaggcggtc ccgaggatgg cgccggtcac ggtccccgca cccccgatga
      121 ggactatggc gatgaactgg accgagagga acagatccca tcgctccggg atcacccggg
      181 agacgtagga ggcgagcagg gcgccggcga tcccggcaaa gaacgaggag atcccgaagg
      241 ccaggagctt gtaggcgaac tcgttgacgc ccatgatctc ggcggcgata tcccggtctc
      301 gtatcgacat gaaggcccgc cccaccctgg tcctctgcag gatcttggcc aggagagtga
      361 agaccacggc gagcagcacc aggaagaaga acgacttggc ggtgcccgag acttcgacac
      421 cgagccaggt gctggtggtg gtgaagtcga tcagcggcgg ttcctccttc caaatccgca
      481 actggagggg agggaagtcc cggcccacct gcgctccacc ggtgagcgaa ttccagtttc
      541 ggaagaagta ttcgccgatg aagaccagac caagggtgac gatggccaag tacagccccc
      601 gcaccctgac cgcggtcggg cccactgcga cgccgacgag cgcagccact atgcccgccg
      661 caggcagcca gatccagatc gggaggccga gcccgatgag aggcccacca accgcgcccc
      721 ccagccagac cgaggtatag gccccgatgc ccatgaagaa ggaatggccc agcgaaacct
      781 ggccggcgag cccggtcagg atgttcagcc cgagggcgcc gacggcgaag atcgccgtcg
      841 tcgagagcag cctcaaccag tcgtcctcgc cgaggaagcc gagcccgggg atgacctgga
      901 acgggatcga gaccaacacc aggaagaaga cgacgagcat gaccctcttc gcggtcgtgt
      961 tgagcagggc ctggtcggcc tcgtagctgg tgtagagcag gggacgacct ctcaacgcct
     1021 tacaccctcc tgatctcggg ggtgccgaac agaccgtaag gtcggatgag caggacgacc
     1081 atcatcaaca gatagcccac gatcccgggg aagcccgagc cgaggaactc gaagccgaga
     1141 tagacgttcg ccgccgcctc ggcgattccc acggtgaagc cgccaaccac tgcgccgacg
     1201 atcgagtcga gcccgccgat gatgaccgcg gggaaggcgc gaaaggcgat gaaagccgtt
     1261 gcctggtcga ctcccgtggc gcgccggggg tacacggaga agaagaggcc ggccaccccg
     1321 gcgagaaagg cgccgatggc ccatgccacc gagaagatgc ggcctacgtt tatcccctgg
     1381 gcccgcgcgg tttcctgatc gaaagcggtg gccctcatcg cgatcccggt gcggctgcgg
     1441 aagaacagcg ccaccgcggc gaacaaggcc accgtcacca ctatctggat cacctccgag
     1501 taggagatcc tgacccatcc caggtcgatc ccgttgagac cccatggtgc gccgatgggc
     1561 cgcggttggg tgccgatgaa gtcgttggtg atcgtccgga ccacgatgtc gattcccagc
     1621 gtgacgatgg cgaccgcgaa caaggcctcg cccaccatgg gccggagaaa gatccgttcc
     1681 aggatcacac cgaacagtgc cgccaccagg agggcggtga cgacgttcgc cgtccactgc
     1741 gcccactccg ggagaaaggt caggtagcgc ccagggaagt cgatgaccgt ggcgaacgca
     1801 gccacgagga aggcgccgag cccggtcagg gcgccgtgcg cgaagttgag caccgacgtc
     1861 gccttgaaga tgatgacgaa gcccatcgcg agcagtgcgt agatggcgcc aaagctgatc
     1921 ccgtccacca gcatctgggc gaactcgttg gggaccgccg tttcctgcgc cagtacgacg
     1981 aagctcggcg tcacgagcga tacatgtctt cgatcagatc accgaacatg tcgctcatcg
     2041 agcttcgctt caacttctgg gtggcggtga gctcaccgtc ttcgtgatcg agctccttgg
     2101 ggatcagccg gaacttctga atcgcctcca ggtgggaatg ggcagcgttc gcctcggaca
     2161 ccacgctttg gatcaggccg atcacttcgg gtt
//
LOCUS       DATITW010000341         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_17360, whole genome shotgun sequence.
ACCESSION   DATITW010000341 DATITW010000000
VERSION     DATITW010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17360"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2321
                     /gene="dnaE"
                     /locus_tag="VLS86_04810"
     CDS             <1..2321
                     /gene="dnaE"
                     /locus_tag="VLS86_04810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HSO49845.1"
                     /translation="YGVIDERVLKPGRVQRPDIDVDFDERYRSEMIRYAAQKYGPDRV
                     AQIVTFSTIKGKQALRDAARVLGHPYGVGDRVAKAMPPSILGREATLAQVMTPPLPDV
                     DSTIKDWYANGQGLRDLYESDPAVRETVDAARGLEGLRRQDSIHAAAVVIAPEPLINI
                     VPIQQKGEVAEVVTQYEMHGVEELGLLKNDFLGLPNLSIIERCLELVEASTGERLDLD
                     SGPLDDVKTFELLQSGNTVGVFQLEGTAMRSLIRSLQPDSFNDVIALVALYRPGPMGA
                     NMHNLYADRKNGRAPIEELHPALTDKLADTYQIMVYQEQVMTVAQELAGYSMVDADNL
                     RHAMGKKIKAVMLAEEEKFVAGCRAQGHTEEVGREIFGLIEHFAGYGFNRSHSAGYGL
                     VAYQTAYLKAHHPAEYLAALLTATKKDKDRTAVYLNECRQMGIKVLVPDVNESDSDFT
                     VLDGRIRFGLSAVRNVGEGVVEKIVEARADGPFLSFQDFVDRVDVSALNKRTVESLIK
                     AGAFDGLGDTRKGLTLVYEQILDATLERRRNEDMGQFSLFAGDSDAVAHNQIQIPDLV
                     WPQKIRLAFEKEMLGLYVSDHPLLSVGPSLAAATSTTVSQLEELADTSNIIVGGLVAS
                     ITRRWTRNGDPMIFFQLEDLEGSVEVVAFPRTVNEYGHAVIEDSVVVVSGYLDQRGDE
                     VKVMAREIKELEIRDNTTVRLKTPAATLTPELVSRLKSILSNHPGSAPVYLNMTSEQG
                     MKVLRLSDAHRVEPRTALFAELKELLGPSAIL"
     gene            complement(2338..>2628)
                     /locus_tag="VLS86_04815"
     CDS             complement(2338..>2628)
                     /locus_tag="VLS86_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HSO49846.1"
                     /translation="MERSYAYSDSISDLPMMEVTGHPVAVNPDSELEAIAHERGWPVV
                     IFARKTKRAVAATALGTVASSTVVLAYALGRRHGRASAMAQFAQQQMTTRRR"
BASE COUNT          494 a          829 c          875 g          430 t
ORIGIN      
        1 cctacggggt gatcgacgag cgcgtcctca aaccgggccg ggtgcagagg ccggacatcg
       61 acgtggactt cgacgagcgc tatcgctccg agatgatccg ctacgcagcc cagaagtacg
      121 ggccggaccg ggtggcgcag atcgtcacct tctccaccat caagggcaag caggcgctgc
      181 gagacgccgc ccgggtcctg gggcatcctt acggggtggg cgaccgggtc gccaaggcaa
      241 tgccgccatc catcctgggg cgggaggcaa cgttggccca ggtgatgacc ccgccgttgc
      301 ccgacgtcga ttcgacgatc aaggactggt acgccaacgg tcagggtctg cgcgacctct
      361 atgagagcga ccccgcggtc agggagaccg tcgacgcggc gcggggtctc gaagggcttc
      421 gtcgtcagga ctcgattcat gccgcggccg tggtgatcgc accagagccg ctgatcaaca
      481 tcgtccccat ccagcaaaag ggtgaggtcg ccgaggtcgt gacccagtac gagatgcacg
      541 gggtggaaga gctagggctg ctcaagaacg acttcctcgg cctgcccaac ctctcgatca
      601 tagagcgctg tctggagctg gtggaggcga gcaccggtga gcggctcgac ctcgactcgg
      661 ggcccttgga cgacgtcaag acgttcgagc tcctgcagtc gggcaacaca gtgggggtct
      721 tccagttgga ggggacggcg atgcgatctc tcatcaggtc gttgcagccg gactcgttca
      781 acgatgtgat cgcgttggtc gctctgtacc gccccggccc aatgggggcg aacatgcaca
      841 acctctacgc cgaccgaaag aacggacggg cgccgataga agagctccat ccggctctca
      901 ccgacaagct cgccgacacc tatcagatca tggtgtatca ggaacaggtg atgaccgtcg
      961 cccaggagct ggcggggtac tcgatggtcg acgccgacaa cctgcgtcac gccatgggga
     1021 agaagatcaa ggcggtgatg ctggccgagg aggagaagtt cgtcgccggc tgccgggccc
     1081 aggggcacac ggaggaagtg gggcgggaga tctttggcct catcgagcac ttcgccggat
     1141 acggattcaa tcgaagccac agcgcgggct acgggctggt ggcctatcag accgcgtacc
     1201 tcaaggccca ccacccagcc gagtacctgg ctgccctcct caccgccacc aagaaggaca
     1261 aggaccggac cgcggtgtac ctcaacgagt gccgccagat ggggatcaag gtgctggtcc
     1321 ccgacgtcaa cgagtccgac tccgacttca ccgtcctcga tgggcggatt cggttcgggc
     1381 tctccgcggt gcggaacgtg ggcgaagggg tagtggagaa gatcgtcgag gctcgcgccg
     1441 atgggccgtt cctgtccttt caggacttcg tcgatcgggt cgacgtctcg gcgctcaaca
     1501 agaggacggt cgagtcgttg atcaaggccg gggcgttcga cggcctgggc gacacgagaa
     1561 agggtctgac cctggtctac gagcagatcc tggatgccac cctggagcga cgtcgcaacg
     1621 aggacatggg ccagttcagc ctgttcgccg gcgattccga cgcggtggcc cacaatcaga
     1681 tccagatccc cgacctggtg tggccgcaga agatccgcct cgccttcgag aaggagatgc
     1741 tcggtctcta cgtttccgac cacccgctgc tctccgtcgg cccctccctg gccgcggcga
     1801 catcgaccac ggtgagccag ctggaggagc tcgcagacac atcgaacatc atcgtcgggg
     1861 gattggtcgc ttcgatcacc cgtcgctgga cccggaatgg cgacccgatg atcttcttcc
     1921 aactcgagga cctcgaaggc tccgtcgagg tggttgcctt cccccggacg gtcaacgaat
     1981 acggccatgc ggtcatcgag gactcggtgg ttgtcgtctc gggatacctc gaccagcgag
     2041 gtgatgaggt caaggtcatg gcccgggaga tcaaggagct ggagatccgg gacaacacga
     2101 cggttcggct caagaccccg gcggcaactc tcaccccgga gctggtgtcg cgactcaagt
     2161 cgattctctc caaccacccc gggtcggcgc cggtctacct caacatgacg agcgagcagg
     2221 ggatgaaggt gctgagattg agcgacgcgc accgggtcga gccgagaacg gcgttgttcg
     2281 cagagctgaa ggagctgctc ggtcccagcg ccatcctctg agaccttcgc cgcgggatca
     2341 gcggcggcgc gtcgtcatct gctgctgggc gaactgagcc atcgccgagg cgcggccatg
     2401 ccggcgaccc agggcgtagg cgaggacgac tgtcgacgac gccaccgtcc ccagcgcggt
     2461 tgccgcgacg gcgcgcttgg tctttcgggc gaagatgacc accggccagc cacgctcatg
     2521 ggctatggcc tcgagctccg agtccgggtt caccgccacc gggtgcccgg tcacctccat
     2581 catcgggaga tccgagatcg agtcggagta ggcatagctc cgctccat
//
LOCUS       DATITW010000342         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_137297, whole genome shotgun sequence.
ACCESSION   DATITW010000342 DATITW010000000
VERSION     DATITW010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137297"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..726)
                     /locus_tag="VLS86_04820"
     CDS             complement(79..726)
                     /locus_tag="VLS86_04820"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HSO49847.1"
                     /translation="MPAASGGVAIRERYGLVLALILVGYLLSAVETSRLVLAINSIIW
                     VSLLLTTLWSPGIPERLRRIGLIATILVLVSTLSLGLLPSDVAQGWSFLILGVAQLAA
                     MLAILNRVTRHRVVTLQTVMGGVAAYALIAFLMAAVFHGADLLTSEPFLNGVVANGDY
                     TYFSFITLTTVGYGDITAASAIAKRLAVVEAFVGQVFIITLVARLVSLWGAPRGD"
     gene            complement(744..1670)
                     /locus_tag="VLS86_04825"
     CDS             complement(744..1670)
                     /locus_tag="VLS86_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HSO49848.1"
                     /translation="MPAASRMKPRRVSGCPSTSAPLQADLSAAGYYTGTIDGLYGPDT
                     IAAVVQLQQDAGLPETGLLDPATQTALAAILARGTSAQVGALQGILISTGYYDGAVDG
                     VWSPAVEQALKDLQGDLGVPATGVVDAATLRAFERALEEAGQAPVTTTVPPPGPTTTQ
                     AAEPTTTQASPTTAPPTSDGILQVLAGNGQFGQFLAAVEAAGLTDMLSGPGPFTVFAP
                     TDEAFAAAGDLPTDPEALSALVLHHVVEGAVSGFDLQGLSSVTTAQGSDLAVSVDQGQ
                     IVLDGISTITVSNVVGSNGLAHVVNAVLVLPG"
     gene            complement(1597..>2040)
                     /locus_tag="VLS86_04830"
     CDS             complement(1597..>2040)
                     /locus_tag="VLS86_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49849.1"
                     /translation="AAAHEQFCSDVQQYVEALGEYGGLFDDVAVTVGDVKNAAQELEP
                     GRDTVVESWQVFQEAVEADPTSGVDIELVEPESIQAVEAAEQAFADASDVRDSTTVVD
                     AGVGFTSAAYQLEVAWVRLFADAGCIEDEAQASQWVSEYVSAPAG"
BASE COUNT          390 a          675 c          662 g          313 t
ORIGIN      
        1 cccgggtcca ggcgctcttg tcgtgggctg aggggaatct acgcagcgca gggcaggatc
       61 agtcacggtg gcggacgatc aatccccgcg tggagcgccc cacagggaga ccaggcgcgc
      121 aaccagcgtg atgatgaaga cctggcctac gaacgcctcc accaccgcca gtcgcttggc
      181 aatggcactt gccgcggtta tgtcgccata acccaccgtt gtgagagtga tgaacgagaa
      241 ataggtgtaa tccccgtttg cgaccacacc gttcaggaac ggctcggaag tcagtaggtc
      301 ggcgccgtgg aacaccgccg ccatcaagaa ggcgatcaag gcgtacgcgg cgacacctcc
      361 catgacggtc tgcaacgtca cgacccggtg tctggtgacc cgattgagga tggcgagcat
      421 cgcagcgagc tgggcgaccc cgagtatcaa gaagctccag ccctgggcaa cgtccgaggg
      481 caacaggccc agcgagagcg tggagacgag aacgaggatc gtggcgatca gcccgatgcg
      541 acgaagacgc tcggggattc cgggggacca cagcgttgtc aacagcaacg acacccagat
      601 gatcgagttg atggccagca cgagacggct cgtctcgacc gcgctgagca ggtagccgac
      661 gaggatcagg gccaacacca agccatatcg ctcgcggatg gcgactcctc ccgaagcagc
      721 cggcatctct gaaaccgaag cggtcatccg gggaggacca gcacagcatt gaccacatgc
      781 gccagtccgt tgctacccac gacattgctc acagtgatgg tggagatgcc atcgaggacg
      841 atctgaccct ggtcaaccga caccgctaga tccgagcctt gggctgtggt gaccgaggac
      901 agaccctgga ggtcgaatcc gctcacggcc ccctcgacca catggtgaag caccagcgcg
      961 ctcagagcct cgggatcggt cggcaggtca ccggcagcgg cgaaggcctc gtcagtaggg
     1021 gcgaacacgg tgaacggccc cggcccggac aacatgtcgg tgagcccggc ggcttcgact
     1081 gcagccagga actggccgaa ctgaccgttc ccggcgagga cctggaggat cccgtcggag
     1141 gttggcggag cggtggtcgg cgacgcctgc gtggtggtcg gctccgcagc ctgcgtggtg
     1201 gtggggcccg ggggaggcac ggtggtcgtc accggagcct gaccggcctc ctcgagagcc
     1261 cgctcgaagg cgcgcagggt tgcggcgtcg acgacaccgg tcgcgggcac cccgaggtcg
     1321 ccctgcagat ccttcaacgc ctgctccacc gctggagacc aaaccccgtc gacggcaccg
     1381 tcgtagtacc cggtgctgat caggatgccc tgcagcgcgc cgacctgagc cgacgtgccc
     1441 cgggcgagga tggcggcgag tgccgtctga gtcgccgggt ccagcagtcc ggtctcgggc
     1501 agaccggcat cctgctgtag ctgcacgacg gcagcgatcg tgtccggacc gtagagcccg
     1561 tcgatggtcc cggtgtagta gccggccgca ctcaggtcag cctgcagggg cgctgacgta
     1621 ctcggacacc cactgactcg cctgggcttc atcctcgatg cagccggcat cggcaaacaa
     1681 gcgcacccag gccacctcga gttggtaggc ggcggaggtg aagccaacgc cggcgtcgac
     1741 cactgtcgtg ctatccctca catcgctggc atcggcgaaa gcctgctctg cagcctcgac
     1801 ggcttggatc gactcgggct cgacgagctc tatatcgact ccgcttgtcg gatctgcctc
     1861 tacagcctcc tggaatacct gccacgactc gaccaccgtg tcacggcccg gctccagctc
     1921 ctgtgccgcg ttcttgacat cacccaccgt gactgccacg tcgtcgaaca accctccata
     1981 ctctcccagg gcctccacgt actgttggac gtccgagcag aactgctcat gtgcggcggc
//
LOCUS       DATITW010000343         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_418055, whole genome shotgun sequence.
ACCESSION   DATITW010000343 DATITW010000000
VERSION     DATITW010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418055"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2145
                     /locus_tag="VLS86_04835"
     CDS             <1..2145
                     /locus_tag="VLS86_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009151822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSO49850.1"
                     /translation="EALEKYGRDLVADARAGKLDPVIGRDAEIRRVVQILSRKTKNNP
                     VLIGDPGVGKTAIVEGLAQRIMRGDVPEGLRDRTLFALDMGALIAGAKYRGEFEERLK
                     AVLGEVKAAEGSIVLFIDELHTVVGAGAAEGAMDAGNMLKPMLARGELHMIGATTLVE
                     YRKYIEKDAALERRFQTVVVDEPSVEDAISILRGLRERFEVFHGVKIQDAALVASAVL
                     SHRYITDRFLPDKAIDLVDEACAMLRTEIDSMPAELDELTRRVTRLEIEEAALAKEKD
                     AASKKRLEELRRELADLRTEAAVMRGQWEAERQTLRRVQEIRAEIERAHREAEDAERA
                     YDLDRAAQLRHGQLPELERRLLAEEETLATEQGQHRLLREVVTEEEIANIVSRWTGIP
                     VSRLQEGEREKLLRLDVLLHERVVGQDEAVQLVADAIIRARSGIKDPRRPIGSFVFLG
                     PTGVGKTELAKALAEALFDTEENMVRIDMSEYQERHTVSRLLGAPPGYVGYEEGGQLT
                     EAVRRKPYSVVLFDEIEKAHADVFNTLLQVLDDGRLTDAQGRSVDFRNTVIIMTSNIG
                     SQYLLEGITGSGEIKPEAREMVLSELRRHFRPEFLNRVDDIVLFKPLTLVEIEKVVDL
                     MLNDVRKRLTEREMTLEVSEDALRFIAEQGFDPVYGARPLRRFIAHEMETRIGRALLG
                     GEVLDGAVVRVELAEGEIAVTYENPGTPATDV"
BASE COUNT          431 a          690 c          807 g          358 t
ORIGIN      
        1 gaggcgctgg agaagtacgg ccgtgatctg gtggccgatg cccgtgccgg gaagctggac
       61 cctgtgatcg ggcgtgacgc cgagatcagg cgggtggtcc agatcctgtc acgcaagacg
      121 aaaaacaacc cggtgctgat cggtgacccg ggggtgggca agactgcgat cgtcgagggc
      181 ctggctcagc gcatcatgcg gggcgacgtg ccggagggcc tgcgcgaccg gacgctgttc
      241 gccctggaca tgggggcgct gatcgcgggg gcgaagtacc gcggtgagtt cgaggagagg
      301 ctgaaggccg tcctcgggga ggtcaaggct gccgaaggca gcatcgtcct cttcatcgat
      361 gagctgcaca cggtggtggg ggcaggggct gccgaggggg cgatggacgc cggcaacatg
      421 ttgaagccca tgctggctcg tggcgagctg cacatgatcg gcgccaccac cctggtcgag
      481 tatcggaagt acatcgagaa ggacgcggcg ctggagcgcc gcttccagac agtggtggtc
      541 gacgagcctt cggtcgagga cgcgatctcg atcctgcgcg gcctccgtga gcgattcgag
      601 gtgttccacg gggtcaagat ccaggatgcg gcgcttgttg cctcggcggt gctcagccac
      661 cggtacatca ccgatcgctt cttgccggac aaggccatcg acctggtcga cgaggcctgc
      721 gccatgcttc gcaccgagat cgactcgatg ccggccgagc tcgacgagct gacccggagg
      781 gtcaccaggc tcgagatcga ggaggccgcc ctcgccaagg aaaaagacgc tgccagcaag
      841 aagcgactgg aagagttgcg acgggagctt gccgacctca ggaccgaagc tgcggtgatg
      901 cgcggacagt gggaggccga gcgtcagacc ctgcgacggg ttcaggagat cagggccgag
      961 atcgagcggg cgcatcggga ggcggaggac gccgaacggg cctacgacct cgatcgggcg
     1021 gcgcagctcc gtcatgggca gcttcccgaa ctggagcgtc ggctcctggc agaagaggag
     1081 acgctcgcca ccgagcaagg gcagcatcgt ctgcttcgtg aggtcgtcac cgaggaggag
     1141 atagccaaca tcgtctccag gtggaccggg atcccggtca gccggctcca ggagggggag
     1201 cgggagaagc tgctccgtct cgatgtcctg ctccacgagc gcgtcgtcgg acaggacgaa
     1261 gccgtccagc tggtggccga cgcgatcatc cgtgcccgct ccgggatcaa ggacccacga
     1321 cgcccgatcg gctcctttgt cttcctcggc cccactgggg tggggaagac cgagctggca
     1381 aaggctctgg ccgaggcgct gttcgacacc gaggagaaca tggtccggat cgacatgagc
     1441 gagtaccagg agcgtcacac cgtgagccgt ctcctcggcg ccccaccggg ctacgtcggc
     1501 tacgaggagg gaggccagct caccgaggcg gtccggcgca agccgtactc ggtggtgctc
     1561 ttcgacgaga tcgagaaggc ccacgcagac gtcttcaaca cgctccttca ggtgctcgac
     1621 gatggccgac tcaccgacgc ccaggggagg tcggtcgatt tccgaaacac cgtgatcatc
     1681 atgacctcga acatcgggtc tcaatacctg ctggagggga tcaccgggtc gggcgagatc
     1741 aagcccgagg cgcgggaaat ggtcctgagc gagttgaggc gacatttccg ccccgagttc
     1801 ctcaaccggg tcgacgacat cgtgctgttc aagccgctca ccctcgtcga gatcgagaag
     1861 gtggtcgatc tgatgttgaa cgacgttcgc aagcgcctca ccgaacggga aatgaccctg
     1921 gaggtgagcg aagacgccct tcgcttcatc gccgagcagg gcttcgaccc cgtgtacggg
     1981 gcacggcccc tccgccgctt catcgcccac gagatggaga ccaggatcgg ccgcgccctg
     2041 ctcggaggcg aggtgctgga tggagccgtg gtccgcgtcg agttggccga aggcgagatc
     2101 gccgtgacct atgagaatcc tggcacgccg gcgacggatg tctgagacac cttttcgcgg
     2161 tgtctctccc accccatagc cgaacaccca gaccgcggct gtgcagcgga tttgggacct
     2221 aaggccctag ccggaacagg ggtttctgga catggtggag acggtcggac agatgtaacc
     2281 ggatac
//
LOCUS       DATITW010000344         3350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_457955, whole genome shotgun sequence.
ACCESSION   DATITW010000344 DATITW010000000
VERSION     DATITW010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457955"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..761
                     /locus_tag="VLS86_04840"
     CDS             192..761
                     /locus_tag="VLS86_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSO49851.1"
                     /translation="MSTRNGVEHESDPELRFEPLFQANYQAIYRYCVRRLGRADAEDA
                     AADVFAVAWRRIDQMPAGESSRAWLFGVAHRVVGNQFRSRRRRTGLSARLGALRGSEL
                     GVDEIESSQSGDVDLLLMALDELSSSDRELLRLSSWDGLTRSEIAYVLGIRENAVDQR
                     LHRARARLKTRFETLDGEASHTETKEAPA"
     gene            758..2590
                     /locus_tag="VLS86_04845"
     CDS             758..2590
                     /locus_tag="VLS86_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49852.1"
                     /translation="MIELLERADPAAGLETDQIRLRAKVEERVGVSAPVRPLSARWAR
                     PWLIAAAAFAAVLVLAVPAVLRQDPRSVFAPRFEGIDDHPGVQDVVALASGGVQTMAV
                     DGDSIWVMTSLQNQLQRISVASGEVEETFTIDAYVEGVVVGGGYVWLNSYDNGGERLR
                     FDPGSGSVDLTIPIGGPAAWFGDSLWISDDEGQVHRISVDGEILSTSSGELKGEGLGY
                     LWVNDPATGLISSLAEDGSRGEIVIPTGPGLDTADGSSVRKVIEAAGDLWLMDDLYPF
                     GTNLSRFDLDTGELSSFAALTFGLADMTEFDGSIWVTSLTDHLLIRVDPESGEVRRFP
                     MPGKTGGVIETDGSLWVTLYHPGALVRLNPNADLIEAGEIVVDDWNRFPHRLLCTGND
                     DAGGPTLILEPIDWIDYGYWSVIQARLSGEGYVVCVNGYVEGEASAEQRASDLAEALA
                     EGRIPGPYVLVAAGDGVNATRLFADGRQDIAGIVLVDPLPVGFQDLVDSLYPTDSPGP
                     PALGLSPAVSESLDGFGDIPLVVIEGDPKAVFLSQQFIDGEGRERAELLDDAWANGLA
                     FYKDLSTDSHSVVAEGTGQHMVIWDQPDLVVAQVLDVLSRSAVP"
     gene            complement(2615..3058)
                     /locus_tag="VLS86_04850"
     CDS             complement(2615..3058)
                     /locus_tag="VLS86_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018809209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HSO49853.1"
                     /translation="MSHQLRVERLIDASPEEVFHAYTDPEAQKVWFTILDEGMIVENQ
                     VDLRVGGEWVSAWGFSPEEMFRETQVFVVVDRPHRLVTKSSGSSPDGMTLETDVEITF
                     QEEGGKTLMTVVQSGFPDEETKDFFARTAWIGFFDRISAYFESRH"
     gene            complement(3055..>3350)
                     /locus_tag="VLS86_04855"
     CDS             complement(3055..>3350)
                     /locus_tag="VLS86_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSO49854.1"
                     /translation="LSDPTRRQILHRLGQGPASISELAQPFGMSLTGIKKHVRILEDA
                     RLVATEKVGRTRRCRLGPEQLDDAVQWIEIYQEQWERRLDGLEAYLEGRKGQS"
BASE COUNT          608 a         1042 c         1057 g          643 t
ORIGIN      
        1 caagacctgg ttggagaccg gtcagaccct caccactccc gggtcgctca tgtacggatc
       61 gggcgagtga aacaacgtct gagcctgagg aatccgctcg tggtcgaagc gatcacttcc
      121 gacggttgaa cgggccgagc caggtaagca cggccgctga cccggacaca taacattgga
      181 gaaaggcggt tgtgtccacg agaaacggcg tcgagcatga atccgatccc gagctccgct
      241 tcgaaccgct gttccaggcg aactatcaag ccatctaccg ctactgcgtc aggcgtttag
      301 gtcgagctga cgccgaggac gctgcggccg atgtctttgc tgtcgcatgg cgacgtatcg
      361 accagatgcc tgccggcgaa tcgagcagag cctggctgtt cggcgtggcc caccgggtgg
      421 tcggcaatca gttccgatct cggcggcgcc gcacagggct gtcggcccga ctcggcgcgt
      481 tgcgaggcag cgaactcggc gtcgacgaga tcgagtcgag ccagagtggc gacgtcgact
      541 tgctgctcat ggccctcgac gagctgagct catcggatcg ggagttgctg cgtctctcct
      601 catgggacgg gttgacgagg agcgagatcg cctatgtcct cggcatcagg gaaaacgcag
      661 tcgaccagcg tcttcaccgc gcccgggcac gactcaaaac ccgattcgag actctcgatg
      721 gagaggcctc tcacacagag acaaaggaag caccagcatg atcgagctac tggagcgcgc
      781 cgaccccgcg gccggcctcg aaacagacca gatccggctt cgggccaaag tagaggagcg
      841 tgtgggtgtc tcggctccgg ttcgtcctct ctctgctaga tgggcccggc cctggctcat
      901 cgccgctgca gcgttcgccg ccgtgctggt gttggccgtc ccggcggtat tgcgccagga
      961 cccgaggtcg gtctttgcac ctcggttcga aggcatcgac gatcaccctg gagtccaaga
     1021 cgtcgtggca ctggcctcag ggggtgtcca gaccatggct gtcgacggcg acagcatctg
     1081 ggtgatgacc tcccttcaga accagcttca gagaatctcg gttgcttcgg gcgaagtgga
     1141 ggagaccttc acgatcgatg catacgtcga aggagtcgtg gtcggtggcg gatatgtttg
     1201 gttgaacagc tacgacaacg gtggagagag acttcgcttc gatcccggat cgggatcagt
     1261 cgatctcacc atcccgatcg ggggcccggc tgcctggttc ggggacagtc tctggatcag
     1321 cgacgacgag ggccaggtcc accggatctc agtcgatggc gagatcctgt ccaccagttc
     1381 cggtgagctg aaaggcgagg gtctgggtta cctatgggtc aacgacccgg cgactggttt
     1441 gatatccagt ctcgccgagg atgggagccg tggagagatc gtgatcccga ccggtccggg
     1501 attggacacg gccgatggat cgagtgttcg caaagtgatc gaggccgcag gcgacctctg
     1561 gctgatggac gacctctacc cgtttggcac caacctgagc aggttcgacc tcgacacagg
     1621 cgagttgagc tcgtttgcgg ccctcacctt cggactggcc gacatgacgg agttcgatgg
     1681 atcgatttgg gtgacttcgc tgaccgacca tctcttgatt cgcgtcgacc ccgagagcgg
     1741 cgaggtgcgc cgcttcccga tgcccggtaa gacaggcggg gtcatcgaaa ccgacggctc
     1801 gttgtgggtc acgctgtacc acccgggggc cttggtgcga ctcaacccga acgccgatct
     1861 gatcgaagcg ggtgagatcg tggtcgacga ctggaaccga ttcccgcatc gtcttctgtg
     1921 caccggaaac gatgacgccg gtggccccac cctcatcctc gagccgatcg actggatcga
     1981 ctacgggtac tggtcggtga tccaggcaag gttgtccggc gagggctatg tggtgtgtgt
     2041 caacgggtac gtcgaaggcg aggcctcggc cgaacagcga gcttcggact tggctgaggc
     2101 actcgccgag ggtcgaatcc cggggcctta cgtcctagtg gccgctgggg acggggtcaa
     2161 tgccacccgt ctcttcgccg acggacgaca agacatcgcc gggattgtcc tggtcgaccc
     2221 gttgccggtc gggttccagg atctcgttga cagtctgtac cccaccgaca gcccaggccc
     2281 tccagccctc gggctttcgc cagctgtgtc cgagtcactc gacggcttcg gcgacatacc
     2341 cctcgtcgtc attgaaggtg accccaaagc tgtcttcctc agccagcagt tcatagatgg
     2401 agaaggtcgt gaacgagccg agctcctcga cgacgcctgg gcgaacgggc tggcgttcta
     2461 caaggaccta tccacggatt ctcactcggt ggtggcagag ggaacaggac aacatatggt
     2521 gatctgggat caacccgacc tcgtcgtggc tcaggtgctc gatgtgctga gccggagcgc
     2581 cgtcccgtag taggtcggaa tcgagaccag ctcgtcaatg cctgctttcg aagtaggcgc
     2641 tgatccggtc gaagaacccg atccatgcgg tgcgggcgaa gaagtccttc gtctcctcgt
     2701 cgggaaagcc actctgcacc acggtcatca gggtcttgcc cccttcctcc tggaaggtga
     2761 tctcgacgtc ggtttccagg gtcatcccgt ccggtgagct gcccgacgac ttggtgacca
     2821 gccgatgcgg tcggtcgacc actacgaaca cctgggtctc gcggaacatc tcctcagggc
     2881 tgaaacccca ggcgctcacc cattccccac ccacccgcag gtcgacctgg ttctcgacga
     2941 tcatcccctc gtccaggatg gtgaaccaga ccttctgcgc ttccgggtcg gtgtaggcgt
     3001 ggaatacctc ttccggcgag gcatcgatca accgctccac cctcaactga tgactcatga
     3061 ctgtcctttc ctgccctcga ggtaggcctc gagaccgtcg agccggcgct cccattgctc
     3121 ttgatagatc tcgatccatt gcactgcatc gtccagctgc tcggggccga gacggcaccg
     3181 ccgggtcctc cccaccttct cggtggccac cagcctggcg tcttccagga tccgcacgtg
     3241 cttcttgatc ccggtcagtg acatcccgaa cggctgcgcc aactcgctga tcgaggccgg
     3301 gccctgaccg agccggtgga ggatctgccg ccgggtgggg tcggaaagcg
//
LOCUS       DATITW010000345         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_578564, whole genome shotgun sequence.
ACCESSION   DATITW010000345 DATITW010000000
VERSION     DATITW010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578564"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VLS86_04860"
     CDS             <1..391
                     /locus_tag="VLS86_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HSO49855.1"
                     /translation="FENAIDKLGTAGVTQGCNPPANDRFCPDRLVTRGQMAAFLVRAL
                     GYSDDGGGDRFSDDDGSVFESAIDKLATAGVTQGCNPPVNDRFCPDDPVTRGQMAAFL
                     KRALGQFYGPTGVEPSMTSPTLRSLAG"
     gene            461..1246
                     /locus_tag="VLS86_04865"
     CDS             461..1246
                     /locus_tag="VLS86_04865"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSO49856.1"
                     /translation="MVDIQLDALATETRRAIYMMLLERPRSVTDIAAELPVSRPAVSQ
                     HLKVLVDAELARVTRAGNRHVYSADPTGMALLRGWVDQMWDMAMGSFAGFATRQMEED
                     MDQADAQKIEPLVKTVHVPGEPGLVFELFTARMGEWWPLDTHSVGGEEAVDARVDPGV
                     GGRVYEVTRDGAEHEWGRVTTWEPAARLAFSWYPGLPSSQATHVEITFRHTVEGTEVT
                     LVHDGWEARGADWEPMRDSYDSGWDYVLGRVPNSMPAGATTSN"
     gene            complement(1266..1547)
                     /locus_tag="VLS86_04870"
     CDS             complement(1266..1547)
                     /locus_tag="VLS86_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49857.1"
                     /translation="MTLDLKALTDGTTLYSIPETGIDPIAESLGPLGPGSKTEAGSPD
                     LGFPDLGTDAGSPLDRFSGWGIWLGWLVLIIGSFPVVLAVDNARRRKTR"
     gene            1591..1935
                     /locus_tag="VLS86_04875"
     CDS             1591..1935
                     /locus_tag="VLS86_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49858.1"
                     /translation="MAAASVVPVDPSEELPGMVVVGSGVVVGKFGIVAWLVAGPGEVV
                     VGEEGGGPSLVGCLKVVVVVRSVVVDPRVVLLVVVGTVVVVVVVVVVVSFPTTLNAVW
                     AHPAPTWQALIV"
BASE COUNT          349 a          649 c          786 g          364 t
ORIGIN      
        1 gttcgagaac gcgatcgaca aactggggac cgccggcgtc acccagggct gcaacccacc
       61 ggccaacgac cggttctgcc ccgaccgact cgtcaccagg ggccagatgg ccgcgttcct
      121 ggtacgcgcc ctgggctaca gcgacgatgg tggtggggat cggttctcgg atgacgatgg
      181 ctcggtgttc gagagtgcca tcgacaagct ggcaacggcc ggcgtcaccc agggctgcaa
      241 tccaccggtc aacgatcggt tctgtccgga cgacccggtg acccggggtc agatggcggc
      301 attcctcaag cgggcccttg gtcagttcta cggcccgacc ggggtcgagc cgtcgatgac
      361 ctcacccacg ctcaggtcgc tcgccggcta gccccgagcc gtccgacaat tgcttaccgt
      421 aagtcttgac ttacggtaag cgaacgctta ccataaggtc atggtcgaca tccagctcga
      481 cgccctggcc acggagacaa gaagagccat ctacatgatg ctcctcgagc gaccgcggtc
      541 ggtgaccgac atcgccgctg agctcccggt gagccgtccg gccgtctccc agcacctcaa
      601 agtcttggtg gacgccgaac tggcccgggt gaccagggcc gggaatcggc acgtgtattc
      661 ggccgatccc accggcatgg cactgctccg gggctgggtg gatcaaatgt gggacatggc
      721 tatggggagc tttgccggct tcgccacgag acagatggag gaagacatgg atcaggcaga
      781 tgcgcagaag atcgagccat tggtcaagac ggtccacgtg cccggagagc cgggtttggt
      841 gttcgagctg ttcacggcgc ggatggggga gtggtggccc ctggacaccc actcggtggg
      901 tggagaagag gcggtcgacg cccgggtgga ccccggtgtc ggcggaaggg tctacgaagt
      961 caccagagac ggtgccgagc atgagtgggg tcgggtgacc acctgggagc cggcagcgcg
     1021 tctcgccttc agctggtacc ccggtctgcc ctcctcccag gcgacgcatg tcgagatcac
     1081 attcaggcac acggtcgaag gcaccgaggt gaccctggtc cacgacggct gggaggcgag
     1141 gggcgccgac tgggagccga tgcgcgacag ctacgacagc ggctgggact acgtgctggg
     1201 ccgggtcccg aactcgatgc cggcgggtgc gacgacctcg aactgaccgg tgagggtcgg
     1261 ctcgctcagc gggtcttacg tcgtctggcg ttgtccacgg cgaggaccac cgggaacgag
     1321 ccgatgatca gaaccagcca gccgagccat atcccccatc cgctgaagcg gtccaacggc
     1381 gacccggcgt cggtccccag gtcggggaaa cccaagtccg gggagccggc ttcggttttc
     1441 gatccgggcc cgagtgggcc gagactctcc gcgatcggat cgatgccggt ttcggggatc
     1501 gagtacaggg tggtgccgtc ggtcagcgcc ttcaggtcca gggtcacggg aaccgacgac
     1561 tggtccggcg gctgaccacc gtcggccagg atggccgcgg cgtccgtggt cccggtggat
     1621 ccatcagaag agctgccggg gatggtggtg gtcggctctg gcgtggttgt ggggaagttc
     1681 gggatcgtcg cctggctggt tgccgggccg ggtgaggtcg tcgtcggaga ggaggggggc
     1741 gggccgtcac tcgtcggctg tctcaaggtc gtggtggtgg tgcgcagcgt ggtcgtcgat
     1801 cccagggtgg tgctgctcgt ggtcgtgggg accgtcgttg tggtggtggt tgttgtcgtg
     1861 gtcgtgtcgt tccctacgac attgaacgcg gtttgggcgc accccgcccc aacctggcag
     1921 gcactgatcg tgtagcgacc cggtgtggcg ccgcccggga tggtgatccc tgcggagagc
     1981 tcgccctgtt cattggtggt ggcggcgccc aggttggagc agccgtcggc ggcccagcac
     2041 agctccaccg tgagcaaggg aagaaatgcg ctgccgctga tggaggctga tgatccggcc
     2101 ggaccggacg ctggcgagat cgagatcgag gcggccgccg caggtgcc
//
LOCUS       DATITW010000346         1942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_618565, whole genome shotgun sequence.
ACCESSION   DATITW010000346 DATITW010000000
VERSION     DATITW010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618565"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VLS86_04880"
     CDS             <1..1013
                     /locus_tag="VLS86_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007254619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSO49859.1"
                     /translation="AALLGAFALIAAACGGDSGGDGDETFTIGVSNTLAGNGWREQMI
                     CAIKAEALASGVVDEVVLQNENTDTTGQIGQIEALISQGVDAIIINPTDREGLNSVIE
                     EAISQDIVVVAVDQAVTAEGAYVATNDQVAYGELGARWLFDQLGGEGNVVEMRGIDGV
                     PADTDRHNGFLAALEDYPGITITQETFTGWDPTTGAQQALDIISAGDVDGIWTSGIDY
                     TVVEQFQNANASFVPIVGADNNGFIDQLINVDGLVGAAVTNPPAIGGVGAAIAIQALT
                     GEEPDQVVTLTPEVFASDDQAALEDLFDPDQQVGWSTYVEIEPWTTYTPDQVSACAGP
                     GE"
     gene            1092..>1942
                     /locus_tag="VLS86_04885"
     CDS             1092..>1942
                     /locus_tag="VLS86_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO49860.1"
                     /translation="MTLLTTRAVAKSFGSVVALRSVDLVVEPGEIHALLGANGAGKST
                     LVKILSGVHSADSGGIEVNGSPVDLVDPTAAMDAGLATVVQDPALIPDLTVRQNLQLT
                     RIDEATVRIWLDRMEVSNLDPDSLIRELPLPLLRLLDLARALANEPSLLMLDEITAAL
                     PVDQAEHVFRVMDDWKGRGKSVLFITHRLAEVLLICDRATILRDGVTAAVLDPRQGGE
                     HALLEAMLGPEVTKEFEAVGQRAVAADAPVVLEARDLHSRGRVNGASFQLRAGEILGV
                     AALEGQG"
BASE COUNT          358 a          608 c          637 g          339 t
ORIGIN      
        1 tggcggctct gctcggagct ttcgccctga ttgccgccgc ttgcggtggc gacagtggag
       61 gtgacgggga cgagacgttc acgatcgggg tgagcaacac cctggccggg aacggttggc
      121 gggaacagat gatctgcgcg atcaaggcgg aggcactggc cagcggggtt gtggacgagg
      181 tagttctcca gaacgagaac accgacacca ccggccaaat cggccagatc gaagctctga
      241 tctctcaggg cgtagacgcc atcatcatca accccaccga ccgtgagggt ctgaactctg
      301 tcatcgagga agccatcagc caggacatcg ttgtcgtggc ggtggaccag gccgttactg
      361 ccgaaggtgc ctatgtggca accaacgatc aggtcgccta tggcgagctc ggtgcacgct
      421 ggctgttcga ccagctcggt ggcgagggca acgtcgtgga gatgcgtggc atcgacggcg
      481 tcccggccga caccgaccgt cacaacggat tcctggccgc gctcgaggac tacccgggaa
      541 tcacaatcac ccaggagacc ttcacggggt gggacccgac gacgggcgct cagcaggccc
      601 tggacatcat cagcgccggt gacgtggacg gcatctggac ttccggaatc gactacacgg
      661 tggtcgagca attccagaac gccaacgcat cgttcgtccc aattgttggt gcagacaaca
      721 acgggttcat cgaccagctg atcaacgtcg acggtttggt cggcgcagca gtgaccaacc
      781 cacccgcgat tggcggcgtc ggtgccgcga ttgcaatcca ggcattgaca ggtgaggagc
      841 cagatcaagt ggtgactctg accccagagg tcttcgcctc tgacgaccag gcggccctcg
      901 aggacctctt cgatcccgac cagcaggtcg gatggagcac ctacgtcgag atcgagccct
      961 ggaccaccta cacgccggat caggtctccg catgtgcggg acctggcgag taatcgagtg
     1021 accgcaaggg ccgggcgccg gggcgcccgg ccctgccaac tctgccggac aatgacgatc
     1081 tcgagggaga aatgaccctg ttgacgaccc gcgccgtggc caagagcttc gggagcgttg
     1141 tcgcgctgcg ttcggtggac ctggttgtcg agccagggga gatccacgcc ctattgggtg
     1201 ccaatggcgc cgggaagagc acgttggtca agatcctctc tggggtccac tccgcggatt
     1261 caggcgggat cgaggtgaac gggtcacctg tcgacctcgt cgatccgaca gccgccatgg
     1321 atgccggtct ggcaacagtc gtccaagacc cggctttgat ccccgacctc acggtccgcc
     1381 agaacctgca gttgacccgg atcgacgaag cgacggtccg gatctggctg gatcgtatgg
     1441 aggtctcgaa cctcgatccc gactcgctga tccgtgagct ccccctcccc ttgttgcgct
     1501 tgctcgacct ggcccgcgcc ctggccaacg agccttcgct cttgatgctg gacgaaatca
     1561 cggccgcgct tccggtcgac caggccgagc acgtgttccg cgtcatggac gactggaaag
     1621 gccgtgggaa gtcggtcctg ttcatcaccc atcgtctcgc cgaagtgctg ctcatctgcg
     1681 atcgggccac gattctccgt gacggtgtca ctgccgcggt gctcgacccg cgccagggcg
     1741 gcgagcatgc cctgctcgag gcgatgctcg gccctgaggt gaccaaggag ttcgaggccg
     1801 tagggcagag ggccgtggcc gccgacgctc cggtcgtcct ggaagccagg gatcttcact
     1861 cgagaggaag ggtcaacggc gcctcgtttc aactcagggc tggtgagatc ctcggtgtcg
     1921 ccgcgttgga gggccagggt ca
//
LOCUS       DATITW010000347         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_297983, whole genome shotgun sequence.
ACCESSION   DATITW010000347 DATITW010000000
VERSION     DATITW010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297983"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VLS86_04890"
     CDS             complement(<1..424)
                     /locus_tag="VLS86_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HSO49861.1"
                     /translation="MGVLTVAGRTEVLVGEGFPDVVLPVREGRQRVAILTQPGATRYA
                     LDIATGLREAGLGVEVVGLPDREEAKTLTVAETVYETLARFGLGRQDTVVGVGGGSVS
                     DLAGFVAGTWLRGVEVVHVPTTLLAAVDAAIGGKTGVNL"
     gene            complement(412..915)
                     /locus_tag="VLS86_04895"
     CDS             complement(412..915)
                     /locus_tag="VLS86_04895"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HSO49862.1"
                     /translation="MTMLLAGMMGSGKTSTGSLAAATLGVSFYDTDVEVTKAFDDTVA
                     ELWDRLGESGFRDLESAVVLKLAGEPAIVATGGGVVLDPENREVMTATGPIVWLQATP
                     EVLMERVGASTGRPLLDRADDRLREIRSLSDERAGSYQDAADHVIDTSGKTIAQVADE
                     IVSLWAS"
     gene            complement(912..>1500)
                     /locus_tag="VLS86_04900"
     CDS             complement(912..>1500)
                     /locus_tag="VLS86_04900"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HSO49863.1"
                     /translation="SASAMIELIEDAKERRDTLGGVFEVIGYGVPAGIGSHVHYDRRL
                     DGMLAAGLMSIPAIKGVEIGDGFEVAFLPGSKSHDEIVSGSTGLERPTNRAGGVEGGM
                     SNGEPIRVRAAMKPISTLMRPLRTVDMSTGEPAEAVRERSDVCAVPAAAVVGEQMVAL
                     TLASEFQRKFGGDTVEEFVAAHRRYVDGLAERLER"
BASE COUNT          257 a          557 c          433 g          253 t
ORIGIN      
        1 cgaggttgac cccggtcttg cctccgatcg cggcgtccac cgcagccagc agggtcgtgg
       61 gcacgtgcac cacctcgaca cctcgcagcc aggtgccggc gacgaacccg gccagatccg
      121 acaccgagcc tccgcctacg ccaaccacgg tgtcctgtcg gcccaggcca aaacgggcca
      181 gggtctcgta gacggtctcg gcaacggtga gggtcttggc ctcctcccgg tcggggaggc
      241 caaccacctc caccccgagg cccgcctccc tgaggcccgt cgcgatatcg agcgcatagc
      301 gcgtcgctcc cggctgggtc aggatggcga ccctctgtct cccctcacga accggtagca
      361 ccacgtccgg gaacccctcc ccgaccaaga cctcggtccg accagcgacg gtcaggacgc
      421 ccataacgac acaatctcgt ccgccacctg ggcgatggtc ttcccgctgg tgtcgatcac
      481 atggtccgcc gcgtcctgat aactgccggc tcgctcatcg gacagggatc gaatctccct
      541 caaacgatcg tcggctctgt cgaggagggg tcgccccgtc gaggctccaa ccctctccat
      601 gagcacttcc ggagtcgcct gcaaccacac gatggggccg gtggccgtca tcacctcccg
      661 gttctccgga tcgagcacca cacctccgcc ggtcgcgact atcgccggtt ctccagccag
      721 cttcaggacc acggcactct ccaagtcccg gaatcccgac tcacccaacc gatcccagag
      781 ctctgcaacg gtgtcatcga acgccttggt gacctcgacg tcggtgtcgt agaagctgac
      841 gccgagagtc gcggcggcca ggcttcccgt agacgttttc ccggatccca tcatcccggc
      901 cagcaacatc gtcatcgctc gagacgctcg gccaatccgt cgacatagcg ccggtgggca
      961 gccacgaact cctcgacggt gtccccaccg aacttgcgtt ggaactcgga ggcgagggtg
     1021 agagcgacca tttgctcccc aacaacggct gcggcgggca ccgcgcacac gtcggatctc
     1081 tctcgcacgg cttcggcggg ctcgcctgtg ctcatgtcga ccgtacggag cgggcgcatc
     1141 agggtggata tcggcttcat ggcggcgcga accctgatcg gctcgccgtt gctcattccg
     1201 ccttcgacgc cgcctgcccg attggtcggc cgttccagcc ccgtgctccc ggagacgatc
     1261 tcgtcatgag actttgatcc ggggaggaaa gcgacctcaa agccgtcacc gatctccacc
     1321 cctttgatcg ccggaatcga catcagcccg gcagccagca tgccgtcgag acggcggtcg
     1381 taatggacgt gcgagccgat tccggcgggg acgccatacc cgatgacttc aaataccccg
     1441 cccagcgtgt cgcgacgctc cttggcgtct tcgatcagct cgatcatcgc cgaggccgat
//
LOCUS       DATITW010000348         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_538661, whole genome shotgun sequence.
ACCESSION   DATITW010000348 DATITW010000000
VERSION     DATITW010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538661"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VLS86_04905"
     CDS             complement(<1..214)
                     /locus_tag="VLS86_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49864.1"
                     /translation="MSSPQTSTRLGSIVTYARARPLVSLLFVVLIGCGLASLALPDSA
                     EVTGTLGIFLGTSASGIVFVRGSRKLV"
     gene            333..749
                     /locus_tag="VLS86_04910"
     CDS             333..749
                     /locus_tag="VLS86_04910"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HSO49865.1"
                     /translation="MEITATDPDSYIATLPEKVRPEIEKLDSLISKVMKGRSRVMWEG
                     VFWGGSEQKIIGYGDFSYQRPKGETIEWFVVGLAAQKNYISLYVTAADEDGYLVKKYA
                     DRLGKAKVGSSALSFKSADDLDLDVLTELLERAKDG"
     gene            790..1086
                     /locus_tag="VLS86_04915"
     CDS             790..1086
                     /locus_tag="VLS86_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49866.1"
                     /translation="MNRARNTALLIGCVLLLAACAAGVNPEVGTAPPDGDVAGFWMGL
                     WHGIIAPITFVISLFTDDVNLYEVHNNGNWYDFGFVLGAGILFGGSAGARMSRR"
     gene            complement(1110..>2400)
                     /gene="ligA"
                     /locus_tag="VLS86_04920"
     CDS             complement(1110..>2400)
                     /gene="ligA"
                     /locus_tag="VLS86_04920"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HSO49867.1"
                     /translation="DVGFRTNPASEELGSIDDVLEYVARAEKGRHQTGYQTDGVVIKV
                     NSLAEQDALGFTSRAPRWAIAYKFPPEEQVTLLKDIMINIGRTGAATPFAVLEPVFVG
                     GATVGMATLHNADQVALKDVRIGDQVIVRRAGDVIPEVVGPVASARTGKEKPWAMPAV
                     CPFCGNPIVRVEGEAVARCTGGFDCPSRVREWLFHFASRGGMDIEHLGYKTIDLLLAE
                     GMIKDPADIFTFDVSRLLGREGWGDTSVGNLSRAIEAAKDRPVARLLTALGIRHVGGT
                     IARTLVRHFGSLTALMDATETEIAEVEGIGPVIARGVAEWSADPANRQLVAKLGDAGV
                     RLADEVETGVAGNLLAGTTFVISGTLDGVSREEAQAAIEQRGGKATNSVSSKTTALIV
                     GESPGASKTRKAEELGVPIIDGDVFRKLLDQGLSALG"
BASE COUNT          429 a          789 c          781 g          401 t
ORIGIN      
        1 cgaccagctt ccgcgacccg cggacgaaca cgatgccgga agccgaggtg ccgaggaaga
       61 taccgagggt gccggtgacc tccgccgagt caggaagtgc caggctggcc agaccacaac
      121 cgatcaggac cacgaacagc agcgagacca gaggtcgggc tcgggcatac gtgacgatcg
      181 atcccagccg ggtgctggtt tggggtgaac tcaccaacat tgatcggcag aaacggcccc
      241 gatctgtaag cgacgtgatt tcgcaccgat ccgttcggtg ccggatcggc aatggtcact
      301 aggttggcgg ccgcacacag aggagggccc ttttggagat cacagcgaca gacccggata
      361 gctacatcgc aacgttgccg gagaaggtgc gtcccgagat cgaaaagctc gattctctga
      421 tcagcaaggt gatgaagggt cgttcccggg tgatgtggga gggcgtgttc tggggaggaa
      481 gcgaacagaa gatcatcggc tacggcgact tctcgtacca aaggccaaag ggggagacga
      541 tcgagtggtt cgtcgtgggg ctggccgctc aaaagaacta catcagcctc tatgtcacgg
      601 ccgccgacga ggacggttac ctggtcaaga agtatgcgga ccggctgggc aaggcgaaag
      661 tgggcagttc tgccctcagc ttcaagtcgg ccgacgatct cgacctcgat gtgctgaccg
      721 agctgctcga gagggccaag gacggatgac cctggcctgg tccgaatgtg aagacagagg
      781 agacaatcca tgaaccgtgc ccgaaacaca gcgctcctga tcggatgcgt gctcctgctg
      841 gcagcgtgcg cggccggcgt caaccccgag gtcggaaccg cgcctcccga cggcgatgtc
      901 gccgggttct ggatgggtct ctggcacggg atcatcgccc cgatcacatt cgtgatctcg
      961 cttttcaccg acgacgtcaa tctctacgag gtgcacaaca acggcaactg gtacgacttc
     1021 gggttcgtgc tcggcgccgg catcctgttc gggggaagcg ccggggcccg gatgagccgg
     1081 cgctagccga aaccgggccg gggacggtct cagcccagag cagacagtcc ctggtcgagg
     1141 agcttgcgga acacgtcgcc gtcgatgatg ggaaccccca gctcctccgc cttgcgggtc
     1201 ttcgaagccc cgggagactc cccgacgatc aaggctgtgg tcttcgagga caccgagttc
     1261 gtggccttgc ccccccgctg ctcgatggcg gcctgcgcct cctcccggct caccccgtcg
     1321 agagtgccgg agatcacgaa cgtcgtcccg gcgagcaggt tcccggccac ccccgtctcg
     1381 acctcgtcgg ccagcctgac cccggcatcg ccgagcttgg cgaccagctg gcgattcgcc
     1441 gggtcggcgg accattcggc gactccccgt gcgatcaccg ggccgattcc ctccacctcg
     1501 gcgatctccg tctccgtggc gtccatcaag gcggtgaggc tcccgaaatg gcggaccagg
     1561 gtccgggcaa tggtccctcc aacgtggcga atcccgaggg cggtgagcag acgcgccacc
     1621 ggccggtcct tggccgcctc gatggcccgg ctcaggttgc caaccgaggt atcgccccag
     1681 ccctcccgcc ccaggagccg gctgacgtcg aaggtgaaga tgtcggcggg gtccttgatc
     1741 atgccttcag cgagcagaag atcgatcgtc ttgtagccga gatgctcgat gtccatcccg
     1801 ccccgtgagg cgaagtggaa caaccactcc cggacccggg acgggcagtc gaaacccccg
     1861 gtgcatcggg cgacagcttc gccctccacg cgaacgatgg ggttgccgca gaacgggcag
     1921 actgccggca tcgcccaggg cttctccttt ccggtgcggg ccgacgcgac cgggcccacg
     1981 acctcgggga tcacgtcacc ggcgcggcgc acgatcacct ggtccccgat ccggacgtct
     2041 ttgagcgcca cctggtcggc gttgtgcaat gtggccatgc ccaccgttgc ccctccgacg
     2101 aataccggtt ccaggacggc gaacggcgtg gccgccccgg tgcgcccgat gttgatcatg
     2161 atgtccttga gcagggtcac ctgctcctcg ggtgggaact tgtaggcgat tgcccaccgc
     2221 ggggccctgg aggtgaaccc gagcgcatcc tgctcggcca gcgagttgac cttgatcacc
     2281 accccgtcgg tctgatagcc ggtctggtgt cggcctttct ccgctcgggc cacgtactcc
     2341 agcacgtcgt cgatgctgcc cagctcctca ctggccgggt tggtccggaa tccgacgtcc
//
LOCUS       DATITW010000349         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_177752, whole genome shotgun sequence.
ACCESSION   DATITW010000349 DATITW010000000
VERSION     DATITW010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177752"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VLS86_04925"
     CDS             <1..811
                     /locus_tag="VLS86_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HSO49868.1"
                     /translation="RGLNVGPREWSRARADALTERAKELGAKGLVWMVVEEDGSLRSP
                     VAKFLGESELSGVAALLEAKPGDVLLLAADRWRVAVEVLGGLRNDLLRPAGHEELAFV
                     MVVDFPLFEEEEDGSITFSHHPFTAPASIEEMRSNPIGAVSKAYDAVLNGVELGSGSV
                     RIHDPVVQRQVFDIIGIDPDTAERRFGWFLEALRYGTPPHAGFAFGIDRLVMVLQGES
                     SIREVIPFPKTQSGVDPMTGAPTRVDDSQLEELGVRLSDQALLRQEEADDS"
     gene            838..2175
                     /locus_tag="VLS86_04930"
     CDS             838..2175
                     /locus_tag="VLS86_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HSO49869.1"
                     /translation="MTDLFAARADELTARAAPLATRMRPRSLEEVVGQPVLMAPGAAF
                     RRLVESGRPVSMLLWGPPGTGKTTLARLVASKADAVFVTLSATSAGVKDIREVLAAAR
                     ARIETEARRTVLFLDEIHRFAKNQQDALLPGVEDGTLVLIGATTENPFFEVNSPLMSR
                     MTLFRLEAVAPQEVAELGRRALADTERGLGERGLEIDPEALDELANRTGGDARQALNA
                     LEVAALLASGDDRRSISLEDVAEALQRRIVRYDKVGDQHYDVISAFIKSVRGSDPDAA
                     LYWLFLMLEGGEDPEFIARRLIILAAEDIGLADSRALPLAVAAAEALAYVGLPEAGYH
                     LAQATIYLAAALKSNSVGRAMARARELVNEGPSPSVPLHLRSTGYPGAEKLGHGAGYK
                     YSHDYEMGVVSQQYFPDGVPPEVIYHPGRSGDEEGIRERMNEVDRVLGREGRD"
     gene            2193..2366
                     /locus_tag="VLS86_04935"
     CDS             2193..2366
                     /locus_tag="VLS86_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49870.1"
                     /translation="MLDRLWWFVTGLVAGGWVTVRALRRRPTPLELRDAALATSADLL
                     DLAARAVRPNRGR"
BASE COUNT          381 a          799 c          851 g          412 t
ORIGIN      
        1 gcgtggcctc aatgtcgggc cccgggaatg gtcccgggcc cgggccgacg ctttgaccga
       61 gcgggctaag gagctgggag caaagggcct ggtttggatg gtggtggagg aggatggctc
      121 cctgcgctca ccggtggcca agttcctcgg cgaatcggag ctgagcggtg tcgctgccct
      181 gctcgaggcc aagccgggtg acgtcctgct cttggccgcc gaccgatggc gggtcgcggt
      241 cgaggtgctg ggtggcctcc gcaacgacct tctccgccct gcgggccatg aggagctggc
      301 tttcgtgatg gtggtcgact tccccctctt cgaggaggag gaggacggtt cgatcacctt
      361 ctcccatcat ccgttcaccg ccccggcctc gatcgaggag atgcggtcca acccgatcgg
      421 cgcggtttcg aaggcctacg acgcggtgct caacggcgtc gaactgggct cgggatcggt
      481 ccgaatccac gatccggtgg tgcagcgcca ggtgttcgac atcatcggga tcgacccgga
      541 cacggccgag cgcagatttg gctggttcct ggaagccctc cgctatggca cccctcccca
      601 cgccgggttc gcctttggca tcgaccgtct ggtcatggtg ctccagggcg agagctcgat
      661 tcgtgaagtc atcccctttc ccaagaccca gagcggggtc gacccgatga cgggcgcccc
      721 cacccgggtc gacgattccc agctggagga gctcggagtg aggctctccg accaggccct
      781 cctgcgccag gaggaagccg acgacagcta ggccaggcgg cggtaccgtc gtcagtgatg
      841 actgacctct ttgcggcgag agccgacgag ctgacggcaa gggccgcacc actcgccacc
      901 cggatgcgcc ctcgatcgct ggaggaggtc gtcggccagc cggttctcat ggctcccggg
      961 gctgcgttca gacgactcgt cgagtcggga cgaccggtgt cgatgctgct ctggggaccg
     1021 cctggaaccg ggaagaccac cctggcccgg ctggtcgcgt cgaaggccga tgcggtcttc
     1081 gtgaccctgt ctgccacctc ggcgggagtc aaggacatcc gtgaggtcct ggccgcggcc
     1141 agggcccgca tcgagaccga ggcacgacgc accgtcctgt tcctggacga gatccaccgc
     1201 ttcgccaaga accagcagga cgccctgctc cctggagtcg aggatgggac attggtcctg
     1261 atcggggcca cgacggagaa cccgttcttc gaggtgaact ccccactgat gagcaggatg
     1321 acgctgttcc ggctcgaagc ggtggcacca caggaggtcg cagagttggg tcgccgcgct
     1381 ctggccgaca ccgagcgcgg gctgggggag aggggattgg agatcgaccc tgaggctctc
     1441 gacgagctcg ccaatcgaac tggcggcgat gcccgtcagg cactcaatgc gctcgaggtg
     1501 gcagccctgc tcgcctcggg ggacgaccgg cgctcgatct cgctcgagga tgtcgccgag
     1561 gctctgcagc gacggatcgt ccgctacgac aaggtgggtg atcagcacta cgacgtgatc
     1621 tcggcgttca tcaagagcgt gcggggatcc gaccccgacg ccgccctcta ctggctgttc
     1681 ctgatgctgg agggcggtga ggaccccgag ttcatcgcgc gaaggctgat catcctcgcc
     1741 gccgaggaca tcggtctcgc cgacagccgc gccctgcccc tggcggtggc cgccgccgag
     1801 gcccttgcct acgtcggtct ccccgaagcc ggataccacc tcgctcaggc gaccatctat
     1861 ctcgccgccg ccctcaagtc gaactcggtg ggaagggcaa tggcccgcgc ccgtgagctg
     1921 gtcaacgaag ggccctcgcc gagtgtgcct ctccatctgc gctcgaccgg ctatccgggc
     1981 gccgagaagc tgggccatgg cgccgggtac aagtactccc acgactacga gatgggagtg
     2041 gtgtcccagc agtacttccc cgacggggtc ccccccgagg tcatctacca cccgggccgg
     2101 agtggcgatg aggagggaat cagggagcgg atgaacgaag tggaccgggt cctggggcgt
     2161 gagggtcggg actgaggggt acgatggccg gcatgctgga tcggctgtgg tggttcgtca
     2221 cgggcttggt cgccggtggt tgggtcacgg tccgcgccct tcggcgccgg cccaccccgc
     2281 tggagctgcg ggacgcggcg ttggccacca gcgcggatct gttggatctg gcggcgaggg
     2341 cggtccgtcc caataggggc cggtagcccc ccggccctcg ttaacctcgt ttctcgccca
     2401 gaaggccggt ccgttggaca gcaatcagat cagaaccgct ttc
//
LOCUS       DATITW010000350         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_658451, whole genome shotgun sequence.
ACCESSION   DATITW010000350 DATITW010000000
VERSION     DATITW010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658451"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(338..958)
                     /locus_tag="VLS86_04940"
     CDS             complement(338..958)
                     /locus_tag="VLS86_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO49871.1"
                     /translation="MPSDGGGSRLVILIHGGFWLGEWERDTMETLAVDLTSRGFATMN
                     LEYRRQGAGGVWPGPGHDVLTAIGRVSQIPEVSAARTAILGHSAGGHLALWAARRGRG
                     RPVELTVGLAPVTDLGAMAGSDGVGSRPAAELLQAGAPPDVDVVPGKTLLVHGADDEV
                     VPVSHSTRLGDGADVVLVEDLGHFEMLDPLRAHWPRVMEALSAALQ"
     gene            complement(1043..1441)
                     /locus_tag="VLS86_04945"
                     /pseudo
     CDS             complement(1043..1441)
                     /locus_tag="VLS86_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013392.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
     gene            complement(1441..1929)
                     /locus_tag="VLS86_04950"
     CDS             complement(1441..1929)
                     /locus_tag="VLS86_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49872.1"
                     /translation="MSFSWAVPEEEPRAGITERFRLIAETMNTAFDSLGIDARIGQLP
                     GEYCPGAWSVNVAGKVKVMGVGQRLVRGAAHVGGVVVVDDGERIRDVLIPVYRALALD
                     WDPRTSGALADRSPGLDNAKVIAAIVTALRERFDLVRGELPDTVLVEARSLLSDHLPR
                     VA"
     gene            complement(1863..>2150)
                     /locus_tag="VLS86_04955"
     CDS             complement(1863..>2150)
                     /locus_tag="VLS86_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49873.1"
                     /translation="ALDVAVSHSTLSAVARGEIPAVFRLNVPGPVVAFGRADRVQPGY
                     AQAVRAAAAHGFGAVERLAGGRRLSSTSRLCPSPGLSPKKSRGPASPSDSA"
BASE COUNT          380 a          746 c          703 g          321 t
ORIGIN      
        1 tcctccccgg ccggggctgg gtcgtcggtg acggtgaggg ctgcgctccc gcccgaagcc
       61 gggttcccgg cgagcagggc gaaggtgagt ccgaccgcgg caccggtgtc gtgacccatg
      121 cttgcggacg cgtcgaagcc ggcgtcggcc tgtgtactcc ccgaagtacc aaacgccgcg
      181 aaacccgcta ccgccaggag tgcgactgcc gcccacccgg cgataaacga aaggcgtctc
      241 aaacgcatat agggtgactc caccgtgggc accggcccac tgcccaagtt ggaaaagcct
      301 accgacttgt ggataagtcc ggcaactcgg aattgggtca ttgcagggcg gcgctcaacg
      361 cctccatcac ccgtggccag tgggcgcgga ggggatccag catctcgaag tgaccgaggt
      421 cttccaccag caccacatcg gcgccgtcgc cgagccgggt ggagtgagac acagggacca
      481 cttcgtcatc agctccatga acgagcagcg tcttgccggg gacgacatcg acgtccggcg
      541 gcgcccctgc ctggagcaac tcggccgccg gacggctgcc gactccatct gatccggcca
      601 tggcgccgag atcggtgacc ggagccagac ccactgtgag ctcgaccggc cgacctctgc
      661 cgcgtctcgc cgcccagagt gcgagatggc caccggctga gtgcccgagg atcgcggttc
      721 tggcggcaga cacctcgggt atctggctga cgcggccgat cgcggtcagc acatcgtgtc
      781 ccggcccggg ccagacacca cccgcaccct gtctccggta ttccagattc atggtggcga
      841 agcctctgct ggtcagatcc acggccagtg tctccatggt gtcgcgctcc cactccccga
      901 gccagaaacc cccgtggatc aagatgacca gccgtgagcc cccgccgtcc gagggcacac
      961 gaaggtcggc gacgttctcg aggtgggggc catagcgaac ggcgtcgacc gggaaggccg
     1021 cccgattgac cagatggcgc aacgcatcgc gataaccggt caaccccctc ccgtagatgg
     1081 ttcggaccac agcctcggag acgagcgagt ctgcccgcca cgtctcgcgc cgtcgcacgt
     1141 tggagatgtg gatctcgaca gtgggagcac ccacggcgcg aatcgcatcc ggtatcgatc
     1201 gcgaggtatg ggtcaatgcg ccgggattga tcacgatccc gtcgagcccg gctgcaccct
     1261 gaatcgcatc taccagctag ccctcgtgat tcgactgatg aaatgtggcg accaccccga
     1321 gctcgtcggc ccatttcgag atctgtgtct ccaggtcggc caggctggtg ctcccgtaga
     1381 ccgccggctc gcgcctgccg agaagattga ggttcggccc gttgaggacc aggacccgca
     1441 tcaagccacc cgaggcaggt gatcggagag gagtgacctg gcttcgacga gcacggtgtc
     1501 cggaagctct ccacgaacca ggtcgaagcg ctcgcgcagc gcggtgacga tggcggcgat
     1561 gaccttggcg ttgtccagac cgggtgagcg atcggcgaga gcccccgacg tccggggatc
     1621 ccagtcgagg gcgagggccc gatacaccgg gatcaggacg tcgcggatcc tctccccgtc
     1681 gtcgacgacc accacccctc cgacgtgggc cgcgcctcgg accagacgct gcccgacccc
     1741 catcaccttg accttgcctg ccacgttgac cgaccatgct cccgggcagt actcgccggg
     1801 aagctgcccg atgcgggcat cgattcccag actgtcgaac gccgtgttca tggtctcggc
     1861 gatcaggcgg aatcgctcgg tgatgccggc ccgcggctct tcttcgggga cagcccagga
     1921 gaaggacaaa gtcgactcgt ggaagacagc cgccgccccc cggccagacg ctccaccgcg
     1981 ccgaagccgt gggcggcggc ggctcgcacc gcctgggcgt agcccggttg caccctgtcg
     2041 gctcgtccga aggcgacgac cgggcccggg acgttgagtc gaaacacagc gggaatctcg
     2101 ccacgggcca cggcggacag ggtggagtgg ctcaccgcga catccagggc
//
LOCUS       DATITW010000351         3458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_58107, whole genome shotgun sequence.
ACCESSION   DATITW010000351 DATITW010000000
VERSION     DATITW010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58107"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VLS86_04960"
     CDS             complement(<1..366)
                     /locus_tag="VLS86_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49874.1"
                     /translation="MATLRGSIQHVVTGKPVRIAAVSFVGVIGLGALLFRLFAPALAA
                     QYLVTLLVPALVMVVFWSLARSRLLEREAATEAAIELESVANRAANAFVVGIAHELRA
                     HRQQCSLRAEALAVAVDYRH"
     gene            443..1312
                     /locus_tag="VLS86_04965"
     CDS             443..1312
                     /locus_tag="VLS86_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HSO49875.1"
                     /translation="MVGKLWSFVRLSRPHFLVGGALMYALGAVTADVVTVGDYLLGQA
                     MVTAGQLTAHYVNEFADYKVDRLVTHRTLFSGGSGVLASGELSRPVALVAALLTSAAA
                     CVLIIFVEPISTTAALLGLISLAVSWLYSLRPVRLLDTGLGEVVTSIVVTVFVPVVGA
                     SVNGGSVTTSLGWAMAGLFPVHLAMMLVFELPDLETDKRAHKKVLAVRMGEGRAHNTI
                     TALLVAAAVLVGAGVATGGLDLAALWALVAAAVPAVVTVREMRGTRYGLLTSSAVATL
                     VVLATGLLVIEIL"
     gene            1450..3216
                     /locus_tag="VLS86_04970"
     CDS             1450..3216
                     /locus_tag="VLS86_04970"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSO49876.1"
                     /translation="MTRWGDRVAHSFKEGLSSVMVAAALAGLIVLGVLAVLIVTVRAA
                     QTTYRAAAMSVSVAEVTERAIATTTALQEERALGSIMSHSALSRYHPSYQDSISRTDE
                     AIQDLRVSWSQHRSQVPETATPPISDVLAAEVSLVDFREATLTPSGETTYPLYSEIVG
                     LSLAATVDLVKQTEDPGVDDWALIGSLLTASEALHNQRYLIQEALVPGGQMSEEARLQ
                     LDVVTSNLNQSLFQARSVAEDDDLLAVEEIITGEPAQEAEGLLASITADDADSSLSGP
                     EEWFEVSSARIAQIADLVPTVHAEVAAVAQGALDDAERALWTRSILLGALFILSILVA
                     SNAVYATRERGEALAEYGQLADGLRQWFVAASFPDAENVEIAARYVPASVRTMSGGDW
                     YDVYEVGEKLAVVIGDIAGHGAEATAQMAQVRNILRGQSTARTLGPAEQVDLLSRSIG
                     DSEIVATLTYGLLDPRSGRFVYTRAGHIPLLIRSGSGEVRIEEEAPGPPIGAGVAIER
                     EQKMTQLLAGDVLILITDGLVEGVDRDIDLALDQIAKSLTEVEHSSEAILDELFAMNE
                     GTPLDDAAALLITWKPLQPQVV"
     gene            complement(3271..>3458)
                     /locus_tag="VLS86_04975"
     CDS             complement(3271..>3458)
                     /locus_tag="VLS86_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSO49877.1"
                     /translation="SLEPLIADLDPKEQEVLFLRFFEDMTQSDIADVVGVSQMQVSRL
                     ISRALDRLRGQLTSGSA"
BASE COUNT          592 a         1123 c         1140 g          603 t
ORIGIN      
        1 atggcgatag tcgacggcaa ctgccagcgc ctcggccctt aggctgcact gttggcgatg
       61 ggctctcagc tcgtgggcga taccgaccac gaacgcgttg gctgcgcgat tggccacgct
      121 ctccaactcg atcgccgcct cggtcgccgc ctcgcgctcg agaagacgag accgagccaa
      181 ggaccagaag acgaccatga ccaacgcagg caccagcagt gtcaccaggt attgagccgc
      241 cagggcaggc gcaaagagcc ggaacagcaa tgcgcccaga ccgatcactc cgacaaatga
      301 aacagccgcg atccgcaccg gcttgccggt gacgacatgc tggatgctcc cgcgtaacgt
      361 tgccacgttg tgtttatcgg ccgttttggg gccggctata gacttcgccc gccacccggg
      421 gacatgaagc gaccacaatt cggtggtggg aaaactctgg tcattcgtcc gcctcagccg
      481 gccacatttc ctggtcggcg gggctttgat gtacgccctc ggcgcggtca ccgcggatgt
      541 ggtcaccgtc ggtgactatc tgctcggcca ggcgatggtc accgcgggac agctcaccgc
      601 tcactatgtg aatgagttcg ccgactacaa ggtggatcga ctggtgactc atcgcaccct
      661 gttctccggc ggcagcggag tgctcgcctc gggcgagctc agccgcccgg ttgccctggt
      721 tgcagcattg ctcaccagtg ctgcggcctg cgtgctgatc atcttcgtcg agccgatctc
      781 gacgacagcg gccctcctcg gcctgatctc gctcgccgtt tcctggttgt actcactgcg
      841 cccggtccgc ctcctggaca cgggtctggg tgaggtggtg acgtcgatcg tggtgacggt
      901 gttcgtaccc gtcgtcggcg cctccgtgaa cggtggctcg gtgaccacga gcctgggctg
      961 ggccatggcc gggttgtttc cggtacatct cgcgatgatg ctcgtcttcg agctgccaga
     1021 cctcgagacc gacaagcgcg cccacaagaa ggtgcttgcg gtgaggatgg gcgagggccg
     1081 ggcccacaac acgatcacgg cgctgttggt ggcagcggcg gtgttggtcg gggccggggt
     1141 ggcgaccggg ggcctggatc tggccgcgct ctgggccctg gtggcggccg ccgtccctgc
     1201 agtggtcacg gtgagggaga tgaggggcac ccgctatgga ttgctgacgt cgtccgcggt
     1261 agccaccctg gtggtgctcg ccaccggtct gctggtgatc gagatcctgt gacgggtgcg
     1321 gccaggcgaa acggtgtccg gccatgcccc atgcctccga cccggaacta ggctcggccc
     1381 agacccgtga ctgaatcgca gcacagcgcc ctcgtccacc cagccgcgtc cggataaggc
     1441 ccggtcgtca tgacacgctg gggggacagg gtggcgcact cgttcaaaga gggtctcagc
     1501 tcggtcatgg tggctgctgc tctggccggc ttgatcgtcc tcggtgtgct cgcggtcctc
     1561 atcgtcaccg tgagagcagc acagaccacg tacagggccg ccgcgatgtc ggtgtcggtg
     1621 gccgaggtga ccgagcgagc gatagcgaca acgacagcgc tccaggagga gcgtgccttg
     1681 ggctcgataa tgagtcacag cgccctgtcc cgatatcacc cctcctacca ggactccatc
     1741 agccgcaccg acgaggcgat acaggatctg cgggtgtcct ggtcccagca tcggtcgcag
     1801 gtcccggaga cggcaacccc gcccatcagc gatgtcctgg cggccgaggt ctcccttgtc
     1861 gactttcggg aggcgaccct gacccccagc ggcgagacca cctatccgct ctactcggag
     1921 atagtcggcc tatccctggc ggcgacggtc gacctcgtga aacagaccga agatccgggc
     1981 gtggacgact gggccctgat cgggtccctg ctcaccgcga gcgaagcgct ccacaaccag
     2041 aggtacctga tccaggaggc tctcgtcccg ggtgggcaga tgtccgagga ggcgcggctc
     2101 caactcgatg tggtcaccag caacctgaac cagagcctgt tccaggcccg ctcggtggcc
     2161 gaggatgacg atctcctggc cgtggaggag atcatcaccg gcgaaccggc tcaggaggcg
     2221 gagggcctcc tcgccagcat cacagccgac gatgccgact cctcgttgtc cggtcccgag
     2281 gagtggttcg aggtcagctc cgcccgaatc gcacagatcg ccgacctcgt cccgacggtg
     2341 cacgcagaag tggctgctgt ggcgcaaggg gccctggacg atgcggagcg cgccctctgg
     2401 acccgctcga tcctcctcgg cgcgctgttc atcctctcga tcctcgtcgc cagcaacgct
     2461 gtctatgcga ccagggaaag aggtgaggca ctcgccgagt acggccagct ggccgacggc
     2521 cttcgtcagt ggttcgttgc cgcctcattc cctgatgccg agaacgtcga gatcgcggct
     2581 cgctacgtcc cggcgtcggt gcgaaccatg tccggcggag attggtacga cgtctacgaa
     2641 gtcggggaaa agcttgcggt ggtcatcggc gacatcgccg gccatggggc tgaggccact
     2701 gcgcagatgg cccaggtgag aaacatcctg cgcgggcagt cgacggcacg cacgctgggg
     2761 ccggcggagc aggtggatct tctcagccgg tcgatcggcg actcggagat cgtcgccacg
     2821 ctcacctacg gtctgctcga ccccaggtcc ggtcgcttcg tctacaccag ggccggccac
     2881 atccctctcc tgatccggtc cgggtcggga gaagtgcgga tcgaggagga ggcacccggg
     2941 ccgccgatcg gtgccggtgt cgccatcgag agggagcaga agatgaccca gcttctggcc
     3001 ggggacgtcc tgatattgat cacggacggc ctggtggaag gtgtggaccg cgacatcgat
     3061 ctcgccctcg atcagatcgc caagtcactc accgaggtcg agcactcctc tgaggcaatc
     3121 ctcgacgagt tgttcgcgat gaacgagggc actccgctcg acgatgctgc tgcccttctc
     3181 atcacctgga agccgctgca gcctcaggtg gtgtgagcca ctcatcgtct ccgccgacac
     3241 aggggccggc caaagacagg ccccttcaac tcaagcggac cccgatgtga gctggcctcg
     3301 gaggcggtcc agggcccgag aaatgagacg ggaaacctgc atctgggaga cacccacgac
     3361 atcggcgatg tccgattggg tcatgtcctc gaagaagcgc aagaagagga cctcttgctc
     3421 cttgggatcg agatcggcga tcagcggctc cagggagt
//
LOCUS       DATITW010000352         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_418630, whole genome shotgun sequence.
ACCESSION   DATITW010000352 DATITW010000000
VERSION     DATITW010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418630"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..764
                     /locus_tag="VLS86_04980"
     CDS             99..764
                     /locus_tag="VLS86_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49878.1"
                     /translation="MTLAQQSAPLIDWDWVARNWGEIVDRTWEHVTLTAVSVGLGLVI
                     SLALSIVALRWRWTLSPITWTTGLLYTIPSLALFALLVPIVGLNATNAVIALTSYTLL
                     ILIRNLVAGVDGVPAPVLEAADGMGYTRRSRFWKMEVPLALPVIVAGLRIATVTTVGL
                     VTVTAVLGLGGYGYFILRGLNTFFWTQIIVGVVLSVVLATILDVAFVMLQRVLSPWSR
                     SPR"
     gene            761..1447
                     /locus_tag="VLS86_04985"
     CDS             761..1447
                     /locus_tag="VLS86_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49879.1"
                     /translation="MTVWESVREFLADPATWSGPGSIWVRLAEHIVVSVVAIVIAAIM
                     VLPIAFWLGHIRKGSDIASAVVNIGRAIPSFGILAISFLILVQMGAGVGEPWAVLVAL
                     VALAAPPMFTNTVAGIQSVEASTIEAARGMGLTEREVLRGVELPLAMPLIMEGVRISF
                     VQVIATATLGALVAWGGLGRFIVDGFAQQDTGQLIVGALLVGILAIVAELGFSRLQKY
                     STPKGLRIAG"
     gene            1537..>2017
                     /locus_tag="VLS86_04990"
     CDS             1537..>2017
                     /locus_tag="VLS86_04990"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HSO49880.1"
                     /translation="MRRIFRARAVGAILALALVATACGSGDGATDGEPITVASFAFNE
                     SVIVAEIYAQGLEAEGYEVERKLNLGNREVVKPALESGEIDLVPEYVGTLAGFLGAEQ
                     SADSQATWENAKAAFEESGVTLLQYSPAQDKNAYVVTAETASELGLTTVSDLQPVAGD
                     "
BASE COUNT          304 a          624 c          698 g          391 t
ORIGIN      
        1 gcggtggtga ccggccacgc ccgggtcgca gtggtcgtcg atgacggggt gtaccgcgga
       61 atgctcgatc tcgaggcgat cgccggggag gtgacggagt gaccctcgcc cagcagtcgg
      121 cgccgttgat cgactgggac tgggtcgccc gcaattgggg cgagatcgtc gatcggacgt
      181 gggagcacgt cacgctgact gcggtgtcgg tcggtttggg tctggtgata tcgctggcgc
      241 tttccatcgt ggctctgcgt tggagatgga ccctgtctcc gatcacatgg accacgggtc
      301 tgctctacac cattccctcg ctcgcccttt tcgccctgct ggtcccgatc gtcgggctca
      361 acgcgaccaa tgccgtgatc gcattgacct cgtacacgtt gctcatcctg atcaggaacc
      421 tggtggcggg ggtcgacggg gtgccggcac cggtgctgga ggccgccgac ggcatgggtt
      481 acacccgccg ctcccgattc tggaagatgg aggttcccct cgcccttccg gtcatcgttg
      541 ccggtttgcg catcgccacc gtgaccacgg tcgggctggt cactgtcacc gcggtcctcg
      601 ggttgggcgg ctacgggtat ttcatcctgc gcggcctcaa caccttcttc tggacccaga
      661 tcatcgtcgg agtggtgctc tcggtggtcc tggccaccat cctcgacgtc gccttcgtga
      721 tgctgcagcg tgtgctcagc ccctggtcga ggagcccgag gtgacggtct gggaatcggt
      781 gcgcgagttc ctcgccgacc ctgccacctg gtcggggccg ggatcgatct gggtgagatt
      841 ggccgagcac atcgtggtct cggtggtcgc catagtgatc gcggcgatca tggtgttgcc
      901 catcgccttc tggctgggtc acatccgcaa gggctcagac attgcgagcg cggtggtcaa
      961 catcggccgg gccatccctt cgttcgggat cctcgccatc tcgtttctga tcctggtcca
     1021 gatgggcgcg ggggtcggcg agccatgggc ggtgttggtg gccctggtcg ccctggccgc
     1081 gcccccgatg ttcaccaaca ccgttgccgg gatccagagt gtcgaggcct cgaccatcga
     1141 ggcggcacgc gggatggggc tgaccgagcg cgaagtgctg cggggggtcg aactgccgct
     1201 ggcaatgccg ctgatcatgg aaggggtccg catcagcttc gtccaggtca tagcaacggc
     1261 caccctgggc gcactggtgg cctggggcgg cctcgggcgc ttcatcgtgg acggattcgc
     1321 ccagcaggac accggccagc tgatcgtggg ggcgctcttg gtggggatcc tggccatagt
     1381 ggccgagctc ggcttctcac gcctgcagaa gtactcgact ccgaagggcc tcagaatcgc
     1441 cggatagtgg gaatcccgat gcaagttcgt cggttgaacc catgccggcc ccgtggatac
     1501 ggggctgggt tgtttaccaa ctagaaggag agacccatgc ggagaatctt ccgcgctcgt
     1561 gccgttgggg cgatcctggc actggccctc gtggccactg cctgcggatc gggtgacggc
     1621 gccactgatg gcgagccgat cacggtcgcg tcgttcgcgt tcaacgagtc ggtgatcgtc
     1681 gccgagatct atgcccaggg cctggaggcc gagggctacg aagtagagcg aaagctcaat
     1741 ctgggcaacc gggaggtcgt gaagcccgcc ctggagtcag gtgagatcga tctcgtgccc
     1801 gaatacgtgg gcaccctcgc cgggttcctg ggtgcggagc agtcggccga ctcgcaagcg
     1861 acgtgggaga acgccaaggc tgcgttcgaa gagagcggcg tcacgcttct ccagtacagc
     1921 cccgcccagg acaagaacgc gtacgtcgtc accgccgaga ccgcatcgga gctgggactg
     1981 accacggtgt ccgacctgca acccgtggct ggagatc
//
LOCUS       DATITW010000353         1850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_378359, whole genome shotgun sequence.
ACCESSION   DATITW010000353 DATITW010000000
VERSION     DATITW010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378359"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VLS86_04995"
     CDS             <1..435
                     /locus_tag="VLS86_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HSO49881.1"
                     /translation="TTFWGYAGQILRTFTIASDPTPRYERLHDVALDAYQAIVEVLRP
                     GATVGEVLDAGQVITEAGFDIWDDLVHGFGGAYLPPIVRTRANRGATHPDDFEYPDGT
                     LLVVQPNVIDGQAGVQTGNSFQVTPAGAVSTQRQPTRMIRCA"
     gene            446..868
                     /locus_tag="VLS86_05000"
     CDS             446..868
                     /locus_tag="VLS86_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO49882.1"
                     /translation="MSDTIAIEMRGITKAFGETVANDGIDFSVRAGEIHVLLGENGAG
                     KTTLMNILFGHMQSDEGVIFVDGDQVEIAAPNDAIAHGIGMVHQHFSLVPSFTVAENV
                     MLGVNRVTDLSFGEDAIEKAVSQRAAELHMPIQPDATV"
     gene            896..>1850
                     /locus_tag="VLS86_05005"
     CDS             896..>1850
                     /locus_tag="VLS86_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO49883.1"
                     /translation="MEIIKAMYRGAKTLILDEPTSLLGPTQIENLLGILSQLRDQGNS
                     IILVTHKLAEVMEVADRVTVLRRGKLVTSMTKGQFDERTLARAMTGHDRERLPDRSDL
                     TGAAPRPLLEIRDLVVPAAHGHGLAIDGLSLMVHSGEILGIAGVEGNGQRELVDALMG
                     LTPPESGMVVVDGVEVTNADPHDRRTAGMGVIPEDRHRLGLILDMSLAENLAVADLAS
                     GRYRRRGRINWKAVHKNAERLLVDYDVRPADPDATAASLSGGNQQKVVLAREMAREPQ
                     VLVADNPSWGLDVGAIDYVHLKLLEMRDRGGAVLLLTLDLEE"
BASE COUNT          348 a          592 c          601 g          309 t
ORIGIN      
        1 accacattct ggggttacgc cggtcagatc ctccgcacat tcacgatcgc ttccgacccg
       61 acgccccgct acgagcggct gcacgacgtg gcgctcgatg cctaccaggc gatcgtcgag
      121 gtcctccgcc ccggtgccac cgtgggtgag gtgctcgacg ccggccaagt gatcaccgag
      181 gcgggtttcg acatctggga cgacctggtc cacggcttcg gcggcgccta cctaccaccc
      241 atcgtccgga ctcgggccaa tcggggggcc acccaccccg acgatttcga atacccggac
      301 ggcacactgc tggtggtgca gccgaacgtg atcgacgggc aggccggggt gcagacgggc
      361 aacagtttcc aggtcacacc cgctggggcc gtcagcaccc agcgtcaacc aacaagaatg
      421 atccgatgtg cgtgagggcg acgacatgag cgacaccatc gcgatcgaga tgcggggcat
      481 caccaaggcg ttcggtgaga cggtggcgaa cgatgggatc gacttctcgg tgcgagctgg
      541 ggagatccat gtcttgctcg gtgagaacgg ggcggggaag accacgctga tgaacatcct
      601 gttcggtcac atgcagtccg acgagggggt gattttcgtc gacggtgacc aggtcgagat
      661 cgccgcgccc aacgacgcca tcgcccacgg tatcggcatg gtccatcagc acttcagcct
      721 cgtgccatcg ttcacggtgg ccgagaacgt gatgttggga gtgaaccgtg tcaccgacct
      781 gtcgttcggc gaggacgcca tcgagaaggc ggtgtcacaa cgggcagccg agttgcacat
      841 gcccatccag cccgacgcca cagtgtaatt cctacccgta gacctccagc agcgggtgga
      901 aatcatcaag gcgatgtacc ggggtgccaa gactctgatc ctggacgagc ccacttcgct
      961 gctcgggccg acccagatcg agaacctcct cggaatcctc tcccaactcc gtgatcaggg
     1021 gaactcgatc atcctcgtca ctcacaagct ggccgaggtg atggaggtcg ccgaccgggt
     1081 aacggtgctc cggcgaggaa aacttgtcac ctccatgacc aagggccaat tcgacgagcg
     1141 caccctggcc cgggcgatga ccgggcacga tcgggaacgg cttcccgatc ggagcgatct
     1201 caccggcgct gctccgcgcc ctctgctgga gatcagagat ctggtggtgc ccgccgctca
     1261 tgggcacggg ctggcgatcg acggtctgtc gctgatggtg cactccgggg agatcctggg
     1321 gatcgcaggc gtcgagggaa acggccagcg cgagttggtc gatgctttga tgggtctcac
     1381 ccccccggag tcagggatgg tggtggtcga cggcgtcgag gtcacaaatg cggacccgca
     1441 tgatcggcgt actgcaggga tgggcgtgat ccccgaagac cgacatcgtc tggggctgat
     1501 cctcgacatg agcctggccg agaatctggc cgtcgccgac ctcgcctccg gccggtaccg
     1561 acggcgcggc aggatcaatt ggaaggcggt gcacaagaat gccgaacgcc tcttggtcga
     1621 ctatgacgtg cgtcccgccg accccgatgc cactgccgcg agcctctccg gcggcaacca
     1681 acagaaggtg gtcctggccc gggagatggc acgcgagcca caagtcctgg ttgccgacaa
     1741 ccccagctgg gggctcgatg ttggagcgat cgactatgtc cacctcaagc tgctcgagat
     1801 gcgcgaccgg ggcggagccg tcctccttct cactctcgac ctcgaggagc
//
LOCUS       DATITW010000354         1765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_258279, whole genome shotgun sequence.
ACCESSION   DATITW010000354 DATITW010000000
VERSION     DATITW010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258279"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VLS86_05010"
     CDS             <1..343
                     /locus_tag="VLS86_05010"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSO49884.1"
                     /translation="ISRMDKVAGALEGGFPESGLTPPFGKDPAALYNITRPPSEYLTR
                     FHYDCCTYSGPALRYLIDTVGIDQVVLGTDYPAPMFLKDPVNWVRGLTELTATEKDAI
                     LATNPGRLLGI"
     gene            340..1347
                     /locus_tag="VLS86_05015"
     CDS             340..1347
                     /locus_tag="VLS86_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSO49885.1"
                     /translation="MTRLGWGVIGLGNIVNTTIAPAMVAEPSCDLVAGVSRDQGRADA
                     FAAKFGARFAYDDYDRMLANPEVEAIFIATPNSLHASQVVAAARAGKHVLCDKPMATN
                     IPDAFLEVQECRRAGVKFGINFHNRHLHWVRDVKEMLAGGVIGVVEAVEVEVGSGTRH
                     YDNWRTDPEMAGLGSIYNVGVHALDFLRLILASEAVEVAAMFDREPGSGAVETLGIVL
                     IRFANGTMAYANCSERLAYPRNDVVFFGSKGRIAGFGLTRARVDGQLTVLTGEGETTT
                     PYPAPGAHQRSVAAFTKAVLSDQEPDASGIDGVRSMQLCEAIARSVAERRVVTVDHTD
                     P"
     gene            complement(1358..>1765)
                     /locus_tag="VLS86_05020"
     CDS             complement(1358..>1765)
                     /locus_tag="VLS86_05020"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HSO49886.1"
                     /translation="LNWEEMQDIVRIRQGLDRAAAEMAVERITVAQIDLAELTCGRLD
                     EESDLGTWVELNEQFHFVFHEATGSWRMTGILKSLEEAAGVYVAQAQRVHPDLRRLAQ
                     RHHRELIEAYRRHDVEAITKIMHDHVALPVEST"
BASE COUNT          336 a          550 c          550 g          329 t
ORIGIN      
        1 catctcgcgg atggacaagg tggcgggagc gttggaaggg ggattccccg agtccgggct
       61 cacccccccg tttgggaagg acccggcggc gctctacaac atcacgagac caccaagcga
      121 gtacctgacc cgcttccact acgactgctg cacctacagc ggacccgccc tgcgctacct
      181 catcgacacc gttgggatcg accaggtcgt cctggggacc gactatcccg caccgatgtt
      241 tctgaaggac ccggttaact gggtgagggg acttaccgag ttaacggcaa ccgaaaagga
      301 cgccatcctc gcaacgaacc cgggccgtct tctgggaata tgacgcgtct cggctggggt
      361 gtgatcggcc tcggcaacat cgtcaataca accatcgccc cggcgatggt tgccgaaccg
      421 agttgcgacc tggtggccgg tgtgagccgc gaccagggac gggccgatgc gttcgcggcc
      481 aagttcggtg ctcgattcgc atacgacgac tacgaccgca tgctggcaaa tcccgaagtc
      541 gaggcgatct tcatcgcgac gcccaattca ctccacgcca gccaggtcgt cgccgcggcc
      601 cgggcaggca agcatgtcct gtgtgacaag ccgatggcaa ccaacatccc cgacgcattt
      661 ctggaagttc aagagtgtcg tcgggctggt gtcaagttcg ggatcaactt ccacaaccgg
      721 caccttcact gggtgcgtga cgtcaaggag atgctcgcag ggggtgtcat cggcgtggtc
      781 gaggcggtcg aggtcgaggt cggatcagga acccgccact acgacaactg gcggaccgat
      841 ccagaaatgg cggggctcgg gagcatttac aacgtcgggg ttcatgccct cgactttctg
      901 cgtttgatac tggcctcaga ggcggtcgag gtggcggcaa tgttcgaccg cgagcccggc
      961 agcggtgcgg ttgagacgct tggcatcgtt ctgatccgct tcgccaacgg aaccatggca
     1021 tacgcgaact gcagcgagcg cctcgcctac ccgcgcaacg acgtcgtctt cttcggttcc
     1081 aaggggcgga tcgccggctt cggcctcacc agggccaggg ttgatggcca actcaccgta
     1141 ttgaccgggg aaggggagac cacaacccct tatccggccc caggggccca tcagcgctcc
     1201 gtggcggcct tcaccaaagc ggtgttatcc gaccaggaac cggacgcctc cggtatcgac
     1261 ggtgtccgaa gtatgcagtt gtgcgaagcc atagcccgat ctgtggcaga gcgccgggtg
     1321 gtcacagtcg atcacaccga cccgtgacgt cgttcagcta agtcgactcg actggaagcg
     1381 ccacgtggtc gtgcatgatt ttcgtgatgg cttcaacgtc gtggcgccgg taggcctcga
     1441 tcagttctcg atgatgcctc tgagccaagc gccggaggtc gggatgaacc ctctgcgcct
     1501 gggcgacgta gaccccggcc gcctcttcga gagacttgag gattccggtc atgcgccacg
     1561 atccggtggc ttcgtggaag acgaagtgga attgctcgtt tagctcaacc caggtgccga
     1621 ggtcggactc ctcgtcgagc cgaccacagg tgagttctgc caggtcgatc tgggcaacgg
     1681 tgattcgctc aacggccatc tcggcagcag ctcgatccaa tccctggcgg atcctgacta
     1741 tgtcctgcat ctcctcccaa ttgag
//
LOCUS       DATITW010000355         1836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_18356, whole genome shotgun sequence.
ACCESSION   DATITW010000355 DATITW010000000
VERSION     DATITW010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18356"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VLS86_05025"
     CDS             complement(<1..870)
                     /locus_tag="VLS86_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HSO49887.1"
                     /translation="MVLVKQVPDVRVGSVAMRPDGTSDRASAAAITNPADLHALEAAL
                     QLADEVWAVSMGPSRAESTLREAIAHGAHRGFLLTDRLFAGSDTWATANSLAAAIRRL
                     GGADLILTGITALDGETGHVGPQVAERLGLPQATGCEELSIEDGRLLARRIIEGGYEL
                     LSMPLPALATVAETGFLPRYPTFPGRQRAAKAEITVLGAADLGLDTATVGLSASPTKV
                     SHMELVPMPKMDCRFIDDEFTYDVLVTELRSLASPAPVSDSAPEVDAEPTKGDAAIGE
                     PKVWVVGEIQDGLL"
     gene            complement(1116..1370)
                     /locus_tag="VLS86_05030"
     CDS             complement(1116..1370)
                     /locus_tag="VLS86_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSO49888.1"
                     /translation="MATNIYVGNLTFNTNSTDLENLFAQHGSVSKAQVVTDRDTGRSR
                     GFGFVEMDSADDAAKAIEALNGKDVDGRQLTVNLAKERSR"
     gene            complement(1586..>1836)
                     /locus_tag="VLS86_05035"
     CDS             complement(1586..>1836)
                     /locus_tag="VLS86_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49889.1"
                     /translation="DQLVGEVSRRFENRWINLWRGDDPIGGHFIEVLESSNWQVCTGN
                     GHSGHEVTPEYGVARSRVLTGTPSWPADQPEPACWGGS"
BASE COUNT          321 a          582 c          582 g          351 t
ORIGIN      
        1 aagcagtccg tcctgtatct cgccaaccac ccacaccttg ggttcgccga tggcggcgtc
       61 ccccttggtg ggctcggcgt cgacctccgg agccgaatct gaaaccggcg ccggcgacgc
      121 gagagaacgg agctcggtaa ccagtacgtc ataggtgaac tcgtcatcga tgaaacggca
      181 gtccattttg ggcatcggca ccagctccat atgggacacc ttcgtgggtg aggcactgag
      241 cccgaccgtg gcggtgtcca gaccgaggtc tgcggcgccg agcaccgtga tctccgcctt
      301 ggccgcccgt tgtcgcccgg gaaatgtcgg gtaacggggg agaaagccgg tctctgccac
      361 cgtggcgagg gcaggcagcg gcatcgaaag gagctcgtat ccgccctcga tgatgcggcg
      421 agcgaggagc ctcccgtctt cgatgctcag ctcttcgcag ccggtggctt gagggagccc
      481 gagtcgctcg gcaacctgag gtccgacgtg accggtttcg ccgtcgagag cagtgattcc
      541 ggtcagtatc agatcggctc caccgagacg acggatggct gctgctagcg agtttgccgt
      601 tgcccacgta tcggagccgg caaacagacg gtcggtgagc agaaacccgc gatgagcccc
      661 gtgggcgatc gcctcgcgca gcgtcgactc cgcccggctc gggcccatcg agaccgccca
      721 tacctcgtcg gccaattgca gtgcagcctc cagcgcatgc aggtcggcgg ggttagtgat
      781 ggcggccgca gaggcgcggt cactcgtccc gtcgggccgc atggccacag agccgactct
      841 tacgtcgggc acctgtttga cgagaaccat cgcccgcatt cgtcacctcc gcttatccga
      901 cgacgatacc gatgccagcc gttgggaaaa gaggcgaaag gcctctcgcg gagaagtgcc
      961 gaccctaacg gtcggtgtct aggttcgacc ctcgcatttg cagcgaggca cggtgcacat
     1021 cgactgacgg tgctccttgt tggggtgccc gcagctgttg cagcgatctg tcttcggctt
     1081 gtagtcgcct cgttttggct taggtaccgc tttgatcaac gagagcgctc tttggccagg
     1141 ttcacggtga gctgacgccc atccacgtcc ttgccattca acgcttcgat tgccttggcg
     1201 gcatcgtcgg ccgagtccat ctcgacgaaa ccgaacccgc gcgagcggcc ggtgtcacga
     1261 tcagtgacca cctgcgcctt gctcacggag ccgtgctgcg cgaagaggtt ttcgaggtcg
     1321 gtcgagttcg tgttgaacgt caggttcccg acatagatgt tcgttgccat gtgtgctgct
     1381 ccttctggaa gctcgaaccg tctgattgct taacgcccca caggtcacct tgcgagagcc
     1441 tcggtcttgg ggagcggggg tctggcatgt gccctgcggc gtcttcaccc gacggaacga
     1501 cagaagcgaa gaaagggcgc agcacgcatc aggcgtgcgc tgtggataac agtctagtcc
     1561 ttcgagggag ccaaggctcg aaccgtcagg atcctcccca acaggcgggt tctggctggt
     1621 ctgccggcca ggatggggtc ccggtgagga ctcgagatct cgcgacgccg tactcggggg
     1681 tcacctcgtg acccgagtgc ccgtttccgg tgcagacctg ccagttcgac gactccagca
     1741 cctcgatgaa gtgccccccg atcgggtcat cgccgcgcca caggttgatc cagcgattct
     1801 cgaatcttct cgaaacctcg ccaaccagct gatcga
//
LOCUS       DATITW010000356         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_338545, whole genome shotgun sequence.
ACCESSION   DATITW010000356 DATITW010000000
VERSION     DATITW010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338545"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VLS86_05040"
     CDS             <1..453
                     /locus_tag="VLS86_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase"
                     /protein_id="HSO49890.1"
                     /translation="FGVEVDAMPGGFVCSASMKGKSTADAVRKVTHRQAKVSSLFSKT
                     QRAFYKEHAPESLKLNDLDALGPINIVKLKFNPKKLGRKMVAELWFYTDGSRILELST
                     KSSPTDAFEVAALTRSFLTDHGVDLSGEQQTKTKIALEHFSSALRADS"
     gene            complement(536..>2232)
                     /locus_tag="VLS86_05045"
     CDS             complement(536..>2232)
                     /locus_tag="VLS86_05045"
                     /inference="COORDINATES: protein motif:HMM:NF021588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HSO49891.1"
                     /translation="KDREPLMRAAGRTAGWLTAAAAASGAMVGVGHEAAAATRPHGRF
                     TGLGAVALTAGGLAGLREFNRRRAENLDAGLALEPSNISAGRSLALAASVTVGSGLVG
                     HAEGLLAERVAMLAARVLPGDADRWRPLGHLATLAAVGYGGRAASARIAGMIEGREKR
                     MEPALDVPPLSAELSGGPDSLIPYNTMSRMGRRFVWTVRRPAEIERVMGEPAIAHPIR
                     AFVSLQAAPSQEERVSLAIEELERAGGFERSWLMVASPTGTGYVNYAAAGALEFLTKG
                     DCATLAMQYSQRPSPLSLDRVSHGRSQFSQLISALSEKLQALPPDRRPRVVMFGESLG
                     AWTSQDAFLGKGTQGLESAGIDYAIWIGTPYGSEWKDQVLDGSGPNVDSSLIGVFDSI
                     DDLRALDAKARKKLRYVMITHTNDGVALFGPELLVKQPDWLGAAETRPRKIPRSQKWT
                     PITSFVQTMIDTKNAARVVPGKFDAEGHDYRADIVPFFDAILGFKQPVDQLVRMAQAL
                     EEEEAFRTAWIADHGEVGKSMASVVLTEVRESHPEVFLEAVYAIRQKRAQSATDGS"
BASE COUNT          388 a          758 c          687 g          399 t
ORIGIN      
        1 ttcggagtgg aagtcgacgc catgccgggt ggctttgtct gctcggcgtc gatgaagggc
       61 aagtcgaccg cagacgcggt gcgcaaggtg acccaccgac aggccaaggt cagctcattg
      121 ttctccaaga cgcagcgcgc cttctacaaa gagcatgccc ctgagtctct gaagctgaac
      181 gacctcgacg ccctggggcc gatcaacatc gtgaagctca agttcaaccc caagaagctg
      241 ggtcgaaaga tggtggccga gctctggttc tacacggacg gttcgaggat cctcgagttg
      301 tccaccaaat cctcaccgac tgatgcgttc gaggtagccg cgttgactcg cagcttcctc
      361 accgaccacg gcgtcgatct cagtggggag cagcagacca agaccaagat cgcgctcgag
      421 cacttttcgt cagcccttcg cgccgactcc tgaccggtgg tgtgctacgg gtagtggcaa
      481 acaccagaag atgccgaacc tcaggagagg atcggatcct gatccgggtc ggcagctagc
      541 tcccgtcggt agccgactga gcccgcttct ggcggatggc gtacacggct tccaagaaca
      601 cctccgggtg gctctccctc acctcggtta ggaccaccga cgccatgctc ttgcccacct
      661 ctccgtggtc tgcgatccag gcggtgcgga aggcctcttc ctcctcgagg gcttgggcca
      721 tccgaaccag ctgatccacc ggctgcttga agcccaggat cgcgtcaaag aagggaacga
      781 tgtcggcccg gtaatcgtgg ccctccgcat cgaacttgcc cgggaccacc cgggccgcgt
      841 tcttggtgtc gatcatggtc tgaacgaaac tggtgattgg agtccacttc tggctcctgg
      901 ggatcttcct gggtcgggtt tccgctgccc ctagccagtc cggttgcttg accaggagct
      961 ccgggccgaa cagggcaacc ccgtcattgg tgtgggtgat catgacgtat cgcagctttt
     1021 tcctcgcctt ggcatcgagc gccctcaggt catctatcga gtcgaacacc ccgatcagag
     1081 aggagtcgac attcggcccc gatccgtcca gcacctggtc cttccactcg ctgccatagg
     1141 gggttccgat ccagatcgcg tagtcgatgc ctgcggattc gagaccctgg gtgcccttgc
     1201 ccaggaaggc gtcttgactg gtccaggccc ccaagctctc tccgaacatg acgacacgtg
     1261 gccgacgatc gggtgggagc gcctgcagct tctcggacaa agccgagatc agctgggaga
     1321 actgggaccg accgtggctg actcgatcca gcgagagcgg cgaaggtcgc tgtgagtact
     1381 gcatggccag ggtggcgcaa tcgcccttgg tcaagaactc caatgcccct gccgccgcgt
     1441 agttgacgta tccggtgccg gtgggggagg cgaccatcag ccaggaccgt tcgaagcctc
     1501 cggctcgctc cagctcctcg atcgccagcg acaccctttc ctcctgggat ggggcggcct
     1561 gcaggctgac gaatgcccgg atcgggtgtg ctatggcggg ctcacccatg accctctcga
     1621 tctccgcggg tctgcgcacc gtccacacga atcgccgacc catccgcgac atcgtgttgt
     1681 acgggatcag gctgtcaggt ccaccgctca actcggccga caggggggga acgtccaacg
     1741 ccggctccat ccgcttctca cggccctcga tcatcccggc aatccgtgcc gaggcggcgc
     1801 gccctccata tccgacggcg gcgagagttg cgagatgccc gagcggccgc cagcggtccg
     1861 cgtcaccggg caacaccctg gccgccagca tggcgaccct ctcggcgagg aggccttcgg
     1921 catgccccac cagcccggag ccgacggtga ccgaagccgc cagggccagc gaccgtccgg
     1981 cggagatgtt ggaaggctcc agggcgagac cggcatcgag gttctcggca cgtcgccggt
     2041 tgaactcccg cagaccagcc agtcctcctg cggtcaatgc aacggctccc agacccgtga
     2101 atcgcccgtg tggcctggtg gctgcggccg cctcatggcc aacaccgacc atggccccgc
     2161 tggccgccgc tgccgcggtc agccatccgg cggtcctgcc cgcggcccgc atcagcggct
     2221 cccggtcttt ct
//
LOCUS       DATITW010000357         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_298597, whole genome shotgun sequence.
ACCESSION   DATITW010000357 DATITW010000000
VERSION     DATITW010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298597"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1633)
                     /gene="atpD"
                     /locus_tag="VLS86_05050"
     CDS             complement(206..1633)
                     /gene="atpD"
                     /locus_tag="VLS86_05050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HSO49892.1"
                     /translation="MSEALSTGRIVKVAGPVIDVEFPPDGLPEILHAITVDFAIGGEK
                     RRVIAEVAQHLGGSKVRAIALAPTDGLTRGAEVVNTGGPITVPVGDQTLGHIFNVWGD
                     SLDAPDQDFSDPRWPIHRTPPAFEDVEPQKTVFETGIKVLDLICPYLQGGKIGLFGGA
                     GVGKTVLIQEMINRVATQHGGVSVFAGVGERTREGTDLFLEMQESGVISKAALVFGQM
                     DEPPGVRLRVALSALTMAEYFRDVQRQDVLLFIDNIFRFTQAGSEVSTLLGRMPSEVG
                     YQPNLADEMGFLQERITSLKGRSITSLQAIYVPADDITDPAPHTAFAHLDATTVLSRP
                     LTALGIYPAVDPLDSSSRALDPLIVGKEHHFVATEVQRVLQRYKDLQDIIAILGIDEL
                     SEEDKLVVGRARRIQRFFAQPMFVAEQFTGQAGVYTPLEETIGAFKAVLDGEMDHYPE
                     QAFYMVGGREDVERRAGELTKGAAA"
     gene            complement(1682..>2247)
                     /locus_tag="VLS86_05055"
     CDS             complement(1682..>2247)
                     /locus_tag="VLS86_05055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HSO49893.1"
                     /translation="DREGKDVILYTVGKKAQAYFRYRGYRIESAYLGVTDTPGYGDAR
                     AIANSVLAAFAKRHIDSVEVLYTRYQSAFIQVPTRFELLPITPPAIAEDSPVRNVSYE
                     FEPSPEEILNRLLPRYVEATIFNVILEASASEHSARRRAMKAATDNAEDLIRVLSVQA
                     NRARQAEITTAISEIVGGAEALAEERR"
BASE COUNT          362 a          716 c          764 g          405 t
ORIGIN      
        1 ctcggcctcg atcgtgaccg gaccggtcac cagggccccc atcagcggct cgtggttggc
       61 catgatcccc agctcaccgt cgggagtctt ggcaaccacc agcgtcgcca gcccactcca
      121 gacggtcgcc tcgggcgaaa ccacgtccac gtgaaaggtg ttggccattg ggatcagccc
      181 cctgccgggt ggtgcgggac ggccactagg cggcggcccc cttggtcagc tcgcctgccc
      241 tccgctcgac gtcctcacga ccacccacca tgtagaacgc ctgctcgggg tagtggtcca
      301 tctcgccgtc gagtaccgcc ttgaaggcgc cgatcgtctc ctcgaggggg gtgtagaccc
      361 cggcctgacc ggtgaactgc tcggcaacga acatcggctg ggcgaagaag cgctggatgc
      421 gccgggcacg gcccaccacg agcttgtcct cctcggagag ctcgtcgatg ccgaggatgg
      481 cgatgatgtc ctggaggtcc ttgtacctct ggaggacgcg ctgcacctcg gtcgccacga
      541 agtgatgctc cttgccgacg atcagcgggt cgagggcccg actcgacgag tcgagggggt
      601 cgaccgcggg gtagatcccg agggcggtca agggccggga gagcaccgtg gtcgcgtcga
      661 gatgggcgaa cgccgtgtgc ggggcggggt cggtgatgtc gtcggccggg acatagattg
      721 cctggagcga tgtgatcgat ctgcccttga gcgatgtgat cctctcctgg aggaaaccca
      781 tctcgtcggc gaggttgggc tggtatccca cctcggacgg catccggccg agcagggtgg
      841 aaacttctga gccggcctgg gtgaagcgga agatgttgtc gatgaacaac agcacgtcct
      901 gacgctgcac gtcccggaag tactcggcca tggtcaacgc ggaaagggca acccgaagcc
      961 ggaccccggg gggctcgtcc atctgaccga agaccagggc ggccttgctg atgacccctg
     1021 actcctgcat ctcgaggaag aggtcggttc cctcacgggt gcgctccccg acccccgcga
     1081 acaccgagac gccaccatgc tgggtggcca cccggttgat catctcctgg atgaggacgg
     1141 tcttgcccac accggcacca ccgaagaggc cgatcttccc accctggaga taggggcaga
     1201 tgagatcgag caccttgatc ccggtctcga acaccgtctt ctgcggctcg acgtcctcga
     1261 aggcgggtgg ggtgcgatgg atgggccatc tcggatcgga gaagtcctga tcgggggcgt
     1321 ccagcgaatc gccccagacg ttgaagatgt ggcccagggt ctggtcgccc accgggacgg
     1381 tgatcgggcc cccggtgttc accacctcgg cgcctctggt caagccgtcg gtgggggcca
     1441 gggcgatcgc ccgcaccttg ctcccgccca gatgctgggc tacctcggcg atgacccttc
     1501 gcttctcgcc gccgatggca aagtcgacgg tgatggcgtg gaggatctcc ggcaacccgt
     1561 cgggtgggaa ctcgacgtcg atcaccggcc cggcgacctt gacgatccgc ccggtgctca
     1621 gggcttcact cattgcattc tccaatcgct tggcatggtc tcatcaatgc ttggggtttg
     1681 gtcatcgccg ctcctcggcg agggcctcgg cgccaccgac gatctcggag atcgcggtcg
     1741 tgatctctgc ctgacgggcg cggttggcct ggacgctgag cacccggatc aggtcttcgg
     1801 cgttgtcggt ggccgctttc atcgcccgcc gccgcgcgct gtgctccgag gccgatgcct
     1861 cgaggatcac gttgaagatg gtcgcctcga cgtatctggg cagcagccgg ttcaggatct
     1921 cctcgggtga gggctcgaac tcatatgaga cgttgcgcac cgggctgtct tcggcgatgg
     1981 ccgggggcgt gatggggagc agctcgaagc gggtcgggac ctggatgaac gccgactggt
     2041 aacgggtgta gagcacctcg accgagtcga tgtggcgttt ggcgaatgcg gccagcaccg
     2101 agttggcgat ggccctggcg tccccgtagc cgggggtatc ggtgacaccg agataggcgc
     2161 tctcgattcg atagccacgg tacctgaagt aggcctgtgc cttcttgccg accgtgtaca
     2221 ggatgacgtc cttgccctca cggtcca
//
LOCUS       DATITW010000358         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_298642, whole genome shotgun sequence.
ACCESSION   DATITW010000358 DATITW010000000
VERSION     DATITW010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298642"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..374
                     /gene="rpsL"
                     /locus_tag="VLS86_05060"
     CDS             3..374
                     /gene="rpsL"
                     /locus_tag="VLS86_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005170078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HSO49894.1"
                     /translation="MPTIEQLVREGRKPKTKKAKAAALGGAPQRRGVCTRVYTVTPKK
                     PNSALRKVCRVRLSSGIEITAYIPGEGHNLQEHSIVLVRGGRVRDLPGVRYKVIRGAL
                     DASGVNDRKQARSRYGSKKDS"
     gene            371..841
                     /gene="rpsG"
                     /locus_tag="VLS86_05065"
     CDS             371..841
                     /gene="rpsG"
                     /locus_tag="VLS86_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HSO49895.1"
                     /translation="MMRRAPAQKRKIEPDPVFRSVLVSKVINKVLWKGKKNTAREIVY
                     GALEVVEQRTSQDPSVVLKRAIDNIRPQVEVRSRRVGGSSYQVPIEVPARRQESLAIR
                     WLVNFARQRREPSMSMRLANDILDASNYTGAAVKRKEDVHKMAESNKAFAHYRW"
     gene            976..>3079
                     /gene="fusA"
                     /locus_tag="VLS86_05070"
     CDS             976..>3079
                     /gene="fusA"
                     /locus_tag="VLS86_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSO49896.1"
                     /translation="MSNGNAAISHLRQVPVSRTRNIGIMAHIDAGKTTLTERILYYTG
                     RNYKIGEVHEGAATMDWMEQEQERGITITSAATTCVWHEHWINIIDTPGHVDFTVEVE
                     RALRVLDGAVAVFDGVAGVEPQSETVWRQADRYGVPRICFINKLDRTGADFYFDIQSI
                     IDRLGARPLVMQLPIGSEADFTGVVDLIEMKAHIWKGDDGKHWDTTDIPADMVDKAAE
                     YREKLLEAVAETSEELLDGYLETGDLTAKEIQEGVRTGTIARDFTPVFCGSAFKNKGV
                     QLLLDAVVAYLPSPADLPAIKGFKPGDPEVVVERNPEDSEPFAALAFKIMSDPHVGKL
                     TYFRVYSGHASQGETVMNTTTGRKERLGRILRMNANAREDIDDIFSGDIVAGIGFKNT
                     TTGDTLSDVGHPIQLESMEFPAPVISVAIEPKTKSDDEKLSESLHRLSEEVPTFLVRT
                     DDETGQTIISGMGELHLEILVDRLLREFKVDATVGKPQVAYRETIRKAIEKVEYRHVK
                     QSGGKGQYGHVVINLEPTGPGGGYEFVDQIKGGKVPREYIPSVDAGIEEALDQGIVAG
                     YPLVDVRAILIDGSYHDVDSSEMAFKIAGSMALQEAARRAGVKLLEPVMEVEVTTPDE
                     YMGDVIGDLNSRRGKVDGMTQRGNSQVIRAQVPMSEMFGYATDLRSKTQGRATYSMQF
                     HSYQDVPDSIAEEIVASRR"
BASE COUNT          638 a          996 c          973 g          472 t
ORIGIN      
        1 acatgccgac gatcgagcag cttgtccgcg agggcaggaa gccaaagacc aagaaggcca
       61 aagcggccgc actgggcggc gccccgcagc ggcgcggcgt gtgcacccgc gtctacacgg
      121 tcacgcccaa gaagccgaac tcggccttgc gcaaggtctg tcgcgttcgt ctctcctcag
      181 gcatcgagat caccgcctac atccccggcg agggtcacaa cctccaggag cacagcatcg
      241 tcctggtccg cggcggccgg gtccgcgacc ttcccggtgt ccggtacaag gtgatccggg
      301 gagccctcga cgcttccggg gtcaacgatc gcaagcaggc tcgcagtcgt tacggatcga
      361 agaaggactc ctgatgcgtc gggcacccgc acagaagcgc aagatcgagc cggatcccgt
      421 attccgcagc gtcctggtca gcaaggtgat caacaaggtg ttgtggaagg gcaagaagaa
      481 caccgcccgg gaaatcgtct acggcgccct cgaagtggtc gagcagcgga ccagccagga
      541 tccgagcgtc gtcctcaagc gtgcgatcga caacatccgt ccccaggtcg aggtccgatc
      601 ccgccgggtc ggtggcagct cctatcaggt cccaatcgaa gtccccgccc gccgccagga
      661 gagcctggcg attcgctggc tggtcaactt cgccagacaa cgccgggagc cgtcgatgtc
      721 gatgcgcctg gccaacgata tcctggacgc ctcgaactac acaggggcgg cagtgaagcg
      781 gaaggaagat gtccacaaga tggcggagtc gaacaaggct ttcgcccact accgctggta
      841 gcaccggtcc caacggagta gcagagaaga tttacccgaa cgttccagca ggcgggctcg
      901 acaccgaaac gagcccgaac cgcaggacgt gggacgttca caacctcata acgagtcgga
      961 gtagtagaag agaagatgag caacggaaac gctgccatat cgcatttgcg ccaggtgccg
     1021 gtgagccgta cccgaaacat cgggatcatg gcccacatcg acgcgggcaa gacgacgctg
     1081 accgagcgca tcctctatta caccggccgt aactacaaga tcggtgaggt ccatgagggc
     1141 gccgccacca tggactggat ggagcaggag caggagcggg ggatcacgat cacctccgcc
     1201 gccaccacct gcgtgtggca cgagcactgg atcaacatca tcgacacccc gggccatgtc
     1261 gacttcaccg tggaggtgga gcgagcgctc cgtgtcctcg acggcgcagt ggccgtattc
     1321 gacggcgtgg ccggagtcga gccccagtcc gagacggtgt ggcgccaggc cgatcgctat
     1381 ggcgtgcccc ggatctgctt catcaacaag ctcgaccgaa ccggagccga cttctacttc
     1441 gacatccagt cgatcatcga tcgtctcggc gcccgacccc tggtcatgca gctccccatc
     1501 ggatccgagg ctgacttcac cggcgtggtc gacctgatcg agatgaaggc ccacatctgg
     1561 aagggtgacg acggcaagca ttgggatacc accgacatcc ctgcggacat ggtcgacaag
     1621 gccgccgagt atcgcgagaa gctgctggag gcagtcgccg agacctcaga ggagctgctc
     1681 gacggctatc tcgagaccgg tgacctcacc gccaaggaga tccaggaggg agtccggacc
     1741 gggaccatcg cccgtgactt caccccggtc ttctgtggct ccgcgttcaa gaacaaaggg
     1801 gtccagttgc tgctggacgc ggtcgtggcc tatctcccca gccccgccga cctccctgcg
     1861 atcaagggat tcaagcccgg cgaccccgag gtcgtcgtcg agcgcaaccc cgaggacagc
     1921 gagccatttg ccgccctcgc tttcaagatc atgtcggacc cgcacgtggg caagctcacc
     1981 tatttcaggg tctactcggg ccacgcctcc caaggggaga ccgtgatgaa caccaccacc
     2041 gggcgcaagg agcgtctggg gcggatcctg cggatgaatg ccaacgcccg tgaggacatc
     2101 gacgacatat tcagcggcga catcgtcgcc gggatcgggt tcaagaacac caccaccggt
     2161 gacaccctgt ccgacgtcgg tcacccgatt cagctcgagt cgatggagtt ccccgccccc
     2221 gtgatctcgg tggcgatcga gcccaagacc aagtcggacg acgagaagct ctccgagtcg
     2281 ctgcaccgtc tctccgagga ggttcccacc ttcctcgtcc gcaccgacga cgagaccggt
     2341 cagaccatca tctcagggat gggcgagctc catctcgaga tcctcgtcga tcgtctgctc
     2401 cgcgagttca aggtggacgc cacggtgggc aagccccagg tcgcctaccg ggagacgatt
     2461 cgcaaggcga tcgagaaggt cgagtatcgc cacgtcaagc agtccggcgg caagggccaa
     2521 tacgggcatg tggtgatcaa cctggagccc accgggcccg ggggcggcta cgagttcgtc
     2581 gaccagatca aggggggcaa ggtgccccgt gagtacatcc cgtccgtcga cgccggaatc
     2641 gaggaagccc tcgaccaggg catcgtcgcc ggctacccgc tggtcgatgt gcgagccatc
     2701 ctcatcgacg ggtcgtacca cgacgtcgac tcgtcggaga tggcgttcaa gatcgcgggg
     2761 tcgatggcgc ttcaggaagc cgctcgtcgg gcaggggtga agttgctcga gccggtcatg
     2821 gaggtcgagg tgaccacacc ggacgagtac atgggcgacg tgatcggaga cctcaacagt
     2881 cgccggggca aggtcgacgg gatgacccag cgggggaaca gccaggtgat ccgggcccag
     2941 gtcccgatgt ccgagatgtt tggctacgcc accgacctgc gctcgaagac ccaagggcgc
     3001 gccacctact cgatgcaatt tcactcatat caggacgtcc ccgactcgat cgccgaggag
     3061 atagtggcga gccggcgcg
//
LOCUS       DATITW010000359         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_619317, whole genome shotgun sequence.
ACCESSION   DATITW010000359 DATITW010000000
VERSION     DATITW010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619317"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VLS86_05075"
     CDS             <1..313
                     /locus_tag="VLS86_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49897.1"
                     /translation="GDNNGPASIQILDSTGSGMPCTWSSDNGQPAGSSSSCTIDISNQ
                     KYNAAWLNIDIPLPDDYTCNLASTTGCWWKVHIETTSGAAHDRTTWTARVSGDPVRWV
                     E"
     gene            314..874
                     /locus_tag="VLS86_05080"
     CDS             314..874
                     /locus_tag="VLS86_05080"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HSO49898.1"
                     /translation="MFRRDRDSGAVAIETALVTGLLLLIVLGAFEYGMGFRSWFGLTA
                     ASREGARVGASVGPADGADCAILEAAAAALASTSENEVVRIKIFEHDAASGTDGAFTS
                     YRPFVDAVDDPLLQRCETWFLEPSTPWTETSRDNTGDDRDWLGVEVVYSHAWITGFLW
                     WNGTVQWTNRSVMRLEPVSYGQVPSP"
     gene            974..1543
                     /locus_tag="VLS86_05085"
     CDS             974..1543
                     /locus_tag="VLS86_05085"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HSO49899.1"
                     /translation="MQGRVSRRRRERGASLVEAALVFPILILIVMGTMEIGLAFKDYL
                     TVSYISREGARLGALAGDDPNADCTILRGIGGLATSKDLARIDEIQIFKADEGTGAQG
                     VTNTATYIEGEDPTICNVPSTPTDGWTINPINWSPTSRQTTVGTDSLDIIGVRVIMTR
                     SWVTNFPPFRGTFQIDESTLTRLEPQAFE"
     gene            1540..>2639
                     /locus_tag="VLS86_05090"
     CDS             1540..>2639
                     /locus_tag="VLS86_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HSO49900.1"
                     /translation="MTRLIKLARRITQPDSEEKGAALPFVAAILVLLLGIAAFAVDLG
                     WLYLNGARLQRAADSSALAGVVYLPGDVPQVVTNAVNGANANGWSVGSVNGAPVGGGG
                     PDELSWRSLADNKLEVTLTANVPTFFLKVLGINDFDVTRIATAEYVKPVPIGSPDPCF
                     GIGNSSLNGADCNPATAQRFWAAISGPLTNKWNGDAHATRFWDDDGTWNQESANTEYR
                     PTGYYLAIDVPTGVSGLNVDVYDAGFYDRGSFDTETGDRVQDTGGGADTHFQFYRFDS
                     TPLDPTDNVVYPSGSCSSSTGRFDIASEASSGTYENRWVRLCRITGAGPAGLYVVRVW
                     TDGNDGGTNQYAVRATTTSGPNARVYGINDIS"
BASE COUNT          491 a          913 c          785 g          450 t
ORIGIN      
        1 cggtgacaac aacggcccgg cttcgattca gatcctggac tcgacaggct cggggatgcc
       61 gtgcacctgg tcttccgaca acgggcaacc ggcggggtcc agctccagct gcacgatcga
      121 tatctcgaac cagaagtaca acgctgcctg gctgaacatc gacatccctc tgcccgacga
      181 ctacacgtgc aacctcgcat ccacgacggg gtgttggtgg aaggtccaca tcgagacgac
      241 gtccggggcg gcccatgacc ggaccacctg gacggctcga gtctccggtg acccggtccg
      301 gtgggtggaa tagatgttcc gccgtgatcg ggactccggg gccgtagcca tcgagactgc
      361 tctggtgacc gggttgctcc tcctcatcgt gctcggtgcc ttcgagtacg ggatgggctt
      421 ccgcagctgg ttcggtctga ccgccgcctc cagggaggga gccagagtgg gggcctcggt
      481 aggcccggcg gacggggccg actgcgccat tctcgaggcg gcggccgccg cactcgcctc
      541 gacatccgag aacgaagtgg tgcggatcaa gatcttcgag cacgacgccg ccagcgggac
      601 cgacggtgct ttcacctcgt accggccctt cgtcgacgct gtcgacgatc cactgctcca
      661 acgctgtgaa acctggttcc tcgagccttc aaccccatgg accgagacgt cccgggacaa
      721 caccggcgac gatcgggatt ggctgggggt ggaggtggtc tacagccacg cctggatcac
      781 cggattcctc tggtggaatg ggaccgtcca gtggactaac cgctcggtga tgcgtctgga
      841 accagtcagc tacggtcagg tccccagccc ctagagctcc cgcacttccc ccttttttct
      901 tgaactgggt cgcgggacct attcagcctt ttctgccttc aggaatggcc cctcgtcaac
      961 cgatagttac ttgatgcagg gacgtgtctc gagacgccgc cgtgagcggg gagcatcgct
     1021 ggtcgaagca gcgttggtct ttccaattct gatcctcatc gttatgggca ccatggagat
     1081 cgggctcgcc ttcaaggact acctcacggt ttcctacatc tcccgtgaag gagcccgtct
     1141 cggcgcactc gccggagacg atcccaatgc cgactgcacc atcctcaggg ggatcggcgg
     1201 tctcgccacc tcaaaggacc tggcacgcat cgacgagatc cagatcttca aagcggacga
     1261 gggcaccggc gcccagggtg tgaccaacac cgccacctac atcgagggcg aggacccgac
     1321 gatctgcaac gtcccctcca cgcccaccga cgggtggacg atcaacccga tcaactggag
     1381 cccgacctca cgccagacca ccgtgggaac cgactccctc gacatcatcg gggtgcgggt
     1441 catcatgacc cggagctggg tcaccaactt cccgccgttc cgcgggacct tccagatcga
     1501 tgaaagcacc ctcacccgac tggaaccaca ggcattcgaa tgaccagact catcaaactc
     1561 gcccgcagga tcactcagcc cgactccgag gagaagggcg ccgcactccc cttcgtcgcc
     1621 gccatcctgg tgctgttgct cggcatcgcc gcctttgccg tcgacctggg atggctgtac
     1681 ctgaacggtg cccgtttgca gcgagccgct gactcctcgg cgctcgccgg cgtcgtctac
     1741 ctgcccggtg acgtccccca agtcgtcacc aacgcggtca acggggcgaa cgccaacggg
     1801 tggtcggtcg gcagcgtcaa cggtgcacct gtaggagggg gcggcccaga cgagctcagc
     1861 tggaggtcgc tcgccgacaa caagctggaa gtgaccctga ccgccaacgt ccccaccttc
     1921 ttcctcaagg tgctcgggat caacgacttc gacgtcaccc gcatcgccac cgctgagtat
     1981 gtcaagccgg tcccgatcgg cagccccgat ccctgctttg gaatcggcaa ctccagcctc
     2041 aacggggctg actgcaaccc ggccaccgca cagcggttct gggcggccat ctccgggcca
     2101 ctgaccaaca aatggaacgg tgacgcccat gccacccggt tctgggatga cgacggcacc
     2161 tggaaccagg agtcggccaa caccgagtac cgccccaccg gctactacct ggcaatcgac
     2221 gtccccaccg gggtgtctgg tctgaacgtg gatgtctacg acgccgggtt ctatgaccgg
     2281 ggcagcttcg acaccgagac cggggaccgg gtacaggaca ccggtggcgg tgccgacacc
     2341 cacttccagt tctaccgatt cgactcgacc ccactcgacc ccaccgacaa tgtcgtctac
     2401 ccctccggat cgtgctccag ctcgaccggc cggttcgaca tcgcctccga agcgagctcg
     2461 ggcacttacg aaaaccgatg ggttcgcctt tgccgaatta cgggagcggg gcccgccggg
     2521 ctctacgtgg tcagagtgtg gaccgacggc aacgacggcg ggaccaacca gtacgcggtg
     2581 cgggccacca ccaccagcgg ccccaacgcc cgtgtctacg ggatcaacga catctcgat
//
LOCUS       DATITW010000360         2723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_58640, whole genome shotgun sequence.
ACCESSION   DATITW010000360 DATITW010000000
VERSION     DATITW010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58640"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VLS86_05095"
     CDS             <1..643
                     /locus_tag="VLS86_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HSO49901.1"
                     /translation="GFNPYMSEGKKTVAHEIARQLDWHMPDAVFVSVGDGCIVGSLWK
                     GFQDLLELGWIGGIPRIVGVQAAGSDFLAEAWENREDVLTKPPIHPETVADSIAAGLP
                     RDRIKAMRAIVQSDGAFVTVTDEEILAAIPTVASHAGVFGEPAAAASWAGVEVARATG
                     VISAGDRIVSISTGTGLKDIPAAMRAVSAAGAHAHHVAPDLDSVATALEDGTR"
     gene            640..2100
                     /locus_tag="VLS86_05100"
                     /pseudo
     CDS             640..2100
                     /locus_tag="VLS86_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886115.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
     gene            2097..>2723
                     /locus_tag="VLS86_05105"
     CDS             2097..>2723
                     /locus_tag="VLS86_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HSO49902.1"
                     /translation="MSRPGITEYLRPASLEEAWRSIADGAPTVRLLSGGSDLTISSPP
                     EVTALVDLGKALNSTVSANDDGSIQIGAGATLTAILEHQSLVDHAGGVIPEMMVRVGS
                     PLLRNSATIGGHLARGRLSDVVPVLIALDAEVRIYRDGVLTPLSLSRYLAEAHNTRPH
                     ILTEVLLPALPASSAAAFHRHARTAFDFPILNACCRVDLGPEDVDQVRI"
BASE COUNT          467 a          973 c          850 g          433 t
ORIGIN      
        1 cgggttcaac ccctacatgt ccgagggaaa gaagaccgtc gcccacgaga tcgcccggca
       61 actcgactgg cacatgccgg atgcggtctt cgtctcggtg ggcgacggat gcatcgtcgg
      121 aagcctgtgg aagggcttcc aagatttgct cgagctgggc tggatcggag gcatcccgag
      181 gatcgtcggg gtgcaggccg ccggttccga cttcctggcg gaagcgtggg agaacaggga
      241 agacgtcctc accaagccgc cgatccaccc cgagaccgtt gccgactcca tcgccgccgg
      301 tctcccccgg gaccggatca aggcgatgcg ggcgatcgtc cagagcgacg gggccttcgt
      361 caccgtcacg gacgaggaga tcctcgccgc catccccacg gtcgcctccc acgccggggt
      421 cttcggcgag ccggccgccg ccgcatcctg ggccggggtc gaggtggcca gggcaactgg
      481 ggtgatctcg gccggcgacc ggatcgtctc catatccacc ggcaccgggc tcaaggacat
      541 ccccgctgct atgagagcgg tctccgccgc cggagctcat gcccatcacg tcgcccccga
      601 cctggactcg gtggccaccg cactggagga cgggacaaga tgatcgatct caccatcagc
      661 gcggccggtc tcgagcacgc ggtctcacgg gcgcgggaac gggacatcgt catccccacc
      721 ttcgcccaca tgcgcgaccc gggcaacatc accgacgagg tggtgcgtcg cctcgaatcc
      781 gtgggtctgt gggacctcga cccgttgaat ctcttccgga tcacctggca caaccagccg
      841 gtggagaagg gtggcggctt cggtgaggtg aacatgatcg agttcccccc agagctgacc
      901 ggggtagatg ccaggatcgt ggccctcgtc ggcaagtggt tccccaccgg ggcccacaag
      961 gttggcgccg ccttcggctg cctcgtcccc cgtctcgtca ccggccagtt cgacccgacc
     1021 acccagaagt cggcctggcc ttcaaccggc aactactgcc ggggcggggc ctacgacgcc
     1081 aacctcctcg cctgcgagtc gatcgccatc ctccccgagg ggatgagcgc cgagcgcttc
     1141 gcctggctgg agaacgtcgc cggagaggtg atcaagaccc cgggctcgga gagcaacgtc
     1201 aaggagatct tcgacaagtg cgccgagttg cgcgcctcgg gataggacat cgtcatcttc
     1261 aaccagttcg acgaattcgg caactacctg gggcattacg cggtgacggg tccggccatg
     1321 ctcgaggtgt tcgagacagt caggggcgag ggcgaccgac tggccgcagt cacctactcc
     1381 acggggtcgg cgggaaccat cgcctcggcc gacttcatca gacagcactt cccgactgtg
     1441 aaggtcgccg cctccgaggc cctccaatgc cccacgttga tgtggaacgg ccacgggagc
     1501 caccgcatcg agggcatcgg cgacaagcac gtcccttgga tcctcaacgt gcgcaacagc
     1561 gacctggtca ccggcatcga cgatgccggg ccaatggctc tgatccggct cttcaacgag
     1621 ccggccggtg tcgactacct cctctcccag ggtgtgccac agaccctggt gacccggctc
     1681 cccgagctgg gcatctcctc gatcgccaac ctgctctcgg cggtcaagct ggccaagtgg
     1741 tacgagatgg ggagcgatga cgtggtgctc accatcgcca ccgactccat ggacatgtac
     1801 cagagccgtc tggtggagga acggggtcgg ctgggtgatt acaccgccac cgacgccgcg
     1861 gtcgcccatc accgctggct gctcgccgag tcgaccgcca acgtgcagga gctggggcac
     1921 tgggaccggc gccgggtaca caacctcaag tacttcacct gggtggaaca acagggcaag
     1981 gaagtctcag agctggatcg ccagtggcac gacccgtcct actgggccga gatccagggc
     2041 atgaccgggc cgatcgaccg tctcatcgaa gacttcaaca ccagggtggc cgcatcgtga
     2101 gccggcccgg catcaccgag tatctgcgcc ccgcgtccct cgaggaggcg tggcgatcca
     2161 tcgccgacgg cgcccccacc gtccggctcc tctccggtgg cagcgacctc accatcagtt
     2221 ccccacccga ggtgaccgcc ttggtcgact tggggaaggc cctcaactcc actgtctccg
     2281 ccaatgatga cggatcgatc cagatcggcg ccggcgccac actgaccgcg atcctggagc
     2341 atcagtcgct cgtcgaccac gccggcggtg tcatccccga gatgatggtg cgggtgggca
     2401 gcccactcct gcgcaactcg gccacgattg gcggtcacct cgcccggggc cggctctccg
     2461 atgtggtgcc ggtgctaatc gccctcgacg ccgaggttcg catctaccgg gatggcgtcc
     2521 tgacgccgct ctccctgtcc cgctacctcg ccgaggcgca caacacccgg ccccacatcc
     2581 tcaccgaagt cctcctcccc gcccttcccg cgagctcggc agccgccttt caccgccacg
     2641 ccaggaccgc cttcgacttt cccatcctca acgcatgctg ccgggtggat ctcggccccg
     2701 aagacgtcga ccaggtccgg atc
//
LOCUS       DATITW010000361         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_419121, whole genome shotgun sequence.
ACCESSION   DATITW010000361 DATITW010000000
VERSION     DATITW010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419121"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..114)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            350..832
                     /locus_tag="VLS86_05110"
     CDS             350..832
                     /locus_tag="VLS86_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49903.1"
                     /translation="MDAIADLLEVDRYRLGEWDETYARLYAIDCASIVYRYHTPDLTD
                     SARDLVQRLLHQTLQLVTAGRENELASIERALMTGLEAASRVTRAVWLVALNAVLPDP
                     FRAAVMSTEGALIAFGRGPNPRRDAVAESLRRRLVSRTEEASLLGSAAPIHRLPSAVA
                     "
     gene            848..1372
                     /locus_tag="VLS86_05115"
     CDS             848..1372
                     /locus_tag="VLS86_05115"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006063403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HSO49904.1"
                     /translation="MTRLEATLADITTVDVDVIVNAANSSLMGGGGVDGAIHTAGGPA
                     ILEECKAIVARRGPLPPGEAVITTAGLLPARHVVHTVGPIWGTMPEGEAVATLASCYR
                     RSLYLAKEAAATTIAFPNISTGVYRFPKELAAEAAVGAVRDWVGQDQGALDRVIFVCF
                     GADNLELYRALLTS"
     gene            complement(1406..2650)
                     /locus_tag="VLS86_05120"
     CDS             complement(1406..2650)
                     /locus_tag="VLS86_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HSO49905.1"
                     /translation="MRTTAVVIGAGHAGLAVSHYLSQRSIDHVVIERGEVANTWRTER
                     WDSLRLLTPNWQSKLPGVGYDGDDPDGYMSMPEVVDFIAGYARTIDAPVQTNTRVTSV
                     RPDSDGYQVVTEQGVISSPTVVLATGGFNLAKVPAVADSVPGSVEMVTPIEYRRPQDL
                     PEGGVLVVGASATGIQIAEEVHRSGRPVTLAVGEHVRLPRTYRGKDVMWWLDRTGILD
                     ERYDEVDDLVRARNLSSPQLVGTPERRNLDLNSLSDQGVRLVGRLAGIADGRAQLSGS
                     LRNKCELADLKLGRLLDTFDEWAFEEGIDGEVDRPHRLSPTRVDASPPLSLDLRSGEI
                     ATIIWATGYRPDYSWLHARVLDRKGQLRHDAGVVTDAPGLYRIGLNLLRRRKSSFIHG
                     ADDDARFVTDHLIAHLGVRASA"
     gene            complement(2670..3230)
                     /locus_tag="VLS86_05125"
     CDS             complement(2670..3230)
                     /locus_tag="VLS86_05125"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSO49906.1"
                     /translation="MTTTDTPVNNGVNVQALLDARTALVDAPEAAQFTWRASTEWVGG
                     THSRSKVENFFGLGGEQSHRSTFTFDADHPEVFASEDNGATPVELVLVGLASCLTAGV
                     AAVAQNREIQLRSVKATIEGNMDILGILGGDPDVRNGFNDIKVKYEIDADASPEDIKA
                     LVAQSQKRSAVFDVITNPTNVTVEVN"
BASE COUNT          609 a         1128 c         1144 g          631 t
ORIGIN      
        1 gagttagctg acggactcgg gcaggaagct cgccctaccc gacgatttcg ccgggattcg
       61 ccccaatact gtggttcccc ccacccgggt ggccgggttc ggcggtaagg ccgagaatct
      121 agccctgccg ggacacgcct aacaatccat ccccgcacat cggccgcgag aagtccgcgg
      181 actagcccca acgcgcgccg ggagaatagg ccatgcggcc catgcccaaa atcggaaaat
      241 ctgtgaatta tccacagggc agatcaaaag cggactgggt aaggagatgg cgatgctggg
      301 atcggaccat gttgaagcgg gcctgacacg gtctagtcaa aaggacgggt tggacgctat
      361 cgccgacctt ctcgaggtcg atcgttaccg gcttggggaa tgggatgaga cctatgcccg
      421 tctctacgcc atcgactgtg cctcgatcgt ctaccggtat cacactcctg atctcaccga
      481 ctccgcccgg gatctggtgc aacgtctcct ccatcagacg ctgcaactgg tcaccgcagg
      541 ccgcgagaac gaattggcct cgatcgagcg ggccttgatg accgggctcg aagccgcatc
      601 cagggtcacc agggcggtat ggctggtcgc cctcaacgca gtcctgcccg atcctttccg
      661 ggcggcggtg atgagcaccg agggtgccct gatcgccttt ggccgtggcc cgaacccgcg
      721 acgggacgcc gtcgccgagt cactgcgccg gcgtctggtg tcgaggaccg aagaggcatc
      781 gcttctcggc tccgcggctc cgatccatcg tctgccctcc gccgtcgcct gacctacagt
      841 ccgggcggtg acccggctcg aggcgaccct ggccgacatc acgacggtcg acgtcgatgt
      901 gatcgtcaac gccgccaact cctccttgat gggcgggggc ggggtggacg gggccatcca
      961 caccgccggc gggccggcga tcctcgaaga gtgcaaggcg atagtcgcca gacgtggccc
     1021 gctgccgccc ggcgaggcgg tcatcaccac ggccgggctg ctccccgccc gccatgtggt
     1081 gcacaccgtc ggcccgatct gggggacgat gccggagggg gaggcggtcg ccacgttggc
     1141 ctcttgctat cggaggagcc tctacctggc aaaggaggcg gcggccacca ccatcgcctt
     1201 tcccaacatc tcgaccgggg tctaccggtt ccccaaggag ttggcggccg aggctgcggt
     1261 cggtgcagtc cgcgactggg tgggccaaga ccagggggcg ttggacaggg tgatattcgt
     1321 ctgcttcggc gcggacaacc tcgagttgta ccgggcactc ctcacgagtt gacagtcgtc
     1381 gtcaacgaca ccgggaggtc agcgctcagg cagaagctct gactccgaga tgagcaatca
     1441 gatgatcggt cacgaatcgg gcgtcgtcgt ctgcgccgtg gatgaagctc gacttgcggc
     1501 gccgaagcag gttcaacccg atccggtaca accccggggc gtcggtcacc accccggcat
     1561 cgtgtcggag ttggcctttg cggtccagga cgcgggcgtg aagccaggag tagtcgggtc
     1621 ggtagccggt tgcccagatg atcgtggcga tctcaccgga cctcaggtcg agagacagcg
     1681 gaggcgaagc gtcgacccgg gttggagaca gtcgatgcgg tcgatcgacc tcaccatcga
     1741 tcccctcctc gaacgcccac tcgtcgaagg tgtcgagcag acgacccagc ttcaggtcgg
     1801 cgagttcgca cttgttccgc agcgatcccg acagctgcgc cctaccgtcc gcgatcccgg
     1861 ccagacgccc aaccaaccgg accccctgat cgctcaggga gttgagatcc aggtttcgcc
     1921 tctcgggggt gcccaccagt tgtggggagg agaggttgcg ggcccgaacc aggtcgtcga
     1981 cttcgtcgta gcgctcgtcg aggatgccgg tccgatccag ccaccacatg acgtctttgc
     2041 ctcggtaggt ccgcgggagc cggacgtgct cgccgaccgc caacgtgacc ggccggcccg
     2101 agcgatggac ctcctcggcg atctgaatgc ctgtggccga tgccccgacg accagaaccc
     2161 ctccctccgg gaggtcttga ggccggcggt attcgatggg ggtgaccatt tcgaccgatc
     2221 ccggcaccga gtcggcgacg gcggggacct tcgccagatt gaaccctccc gtcgccagca
     2281 cgaccgtcgg gctggagatg accccttgtt cggtgactac ctgatagccg tcgctatcgg
     2341 gacgcaccga cgtgacccgc gtgttcgtct gaaccggagc gtcgatcgtc cgggcgtagc
     2401 cagcgatgaa gtcgaccacc tcgggcatcg acatgtaacc gtcgggatcg tcaccgtcgt
     2461 aacccacacc gggcagcttg ctctgccaat tcggggtgag caggcgcagc gaatcccagc
     2521 gttcggtgcg ccaggtgttc gccacctcgc cacgctcgat gaccacatgg tcgatcgagc
     2581 gttggctcag gtagtggctg acagccagcc cggcgtgacc ggcgccgatc acgaccgcag
     2641 tcgtgcgcat tccactcagt tcggttggct cagttgacct cgacggtcac gttggtcggg
     2701 ttggtgatga cgtcgaagac ggcggagcgc ttctgcgatt gcgccaccag cgccttgatg
     2761 tcctcaggtg aggcatcggc atcgatctcg tacttgacct tgatgtcgtt gaacccgttt
     2821 ctcacatccg gatcgccgcc gagaatgcca aggatgtcca tgttgccttc gatcgtggcc
     2881 ttcaccgatc gcagttggat ctcccgattc tgtgcgaccg cggcgactcc ggccgtcagg
     2941 caactggcca gaccgacgag gaccagctcg acgggggtgg cgccgttgtc ctcggaggcg
     3001 aatacctcgg gatggtcggc atcgaaggtg aaggttgagc ggtgtgactg ctcgccgccg
     3061 aggccgaaga agttctcgac cttcgaacgg ctatgcgtgc caccgaccca ttcggtgctg
     3121 gcccgccatg tgaactgggc ggcttctggt gcgtcgacga gagcagtgcg agcatcgagc
     3181 aatgcctgca cattgacccc gttgttgacc ggggtgtcgg ttgtggtcat gtttcttcct
     3241 cctgatcgtt gatgatgtcg gtgcctggtg aaccggggcc ttgttgaaac gcctgcgaag
     3301 gacaaccttc gatgcgctct cgggcggttg gcgatcgcta acagcgagct tcgcagctag
     3361 tcaggccacc cgagagcccg aacaaatgca ccaaggccca gggccggccg cggtcggtcc
     3421 cgaagacaac cgcattctgt tgtgagttcg cgtcactggt tcgccttggg tcaaccgggc
     3481 agagcgcgga agtagagcag atgacctgcg ac
//
LOCUS       DATITW010000362         4616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_699502, whole genome shotgun sequence.
ACCESSION   DATITW010000362 DATITW010000000
VERSION     DATITW010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699502"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1749)
                     /gene="nuoL"
                     /locus_tag="VLS86_05130"
     CDS             complement(<1..1749)
                     /gene="nuoL"
                     /locus_tag="VLS86_05130"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSO49907.1"
                     /translation="MTDFIWLVIALPLGGALFLHFLGRYLREPLAGWVATAAIGGSFA
                     IAAVAAIPFFEGGESGTIVSLWEWMPAIGANLELLWDPLAALMALVVTGVGALIHVFA
                     IGYMHGDPRFSRFFTYLNLFAASMLTLVLAGNYAMLFLGWELVGLCSYLLIGFWFTRP
                     TAAAAAKKAFIVNRIGDFGFMVGLMLVFATFGTLSYSGIFERAGAELTSGMATAIGLL
                     ILVGAAGKSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYVIARSAAIYELTPLA
                     STVVATVGAITALWAASIAMAQRDIKRVLAYSTISQLGYMFLAVGATAYVAGIFHLMT
                     HAFFKALLFLGAGSVIHAMHDEQDMHKMGGLFKKMPVTAITMGIATLAIAGIPPLAGF
                     WSKDEILASSFARGGWFSALWIVGLLTALLTAFYMSRQWVLVFLGEPRWDDLAHPHES
                     PRVMTLPLVVLAALSVVGGLVNTPFRTSLEHFLEPSFELVHVQHPDEGWLMFALLAGL
                     SVLAAVVGIGVGFLTYRRPKEGWRRFEESFEPLWGTWEQAYGVDDLYGRVLVAPGRKA
                     AEVAAFDIDLPLVDGAV"
     gene            complement(1752..2054)
                     /gene="nuoK"
                     /locus_tag="VLS86_05135"
     CDS             complement(1752..2054)
                     /gene="nuoK"
                     /locus_tag="VLS86_05135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HSO49908.1"
                     /translation="MVSVDWYMALAAALFLIGALGMLLRRNALVMFMCIELMLNAVNL
                     TLISAGRAMNDINGQVAVFFILVVAAAEVVVGLAIIVAIFRRRTSASVDELNELRG"
     gene            complement(2054..2578)
                     /locus_tag="VLS86_05140"
     CDS             complement(2054..2578)
                     /locus_tag="VLS86_05140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HSO49909.1"
                     /translation="MAEAILFVLMGITALAGAVTVVIARNPVFSAMGLLGTLFSVAVL
                     YVVQLAHLVAAVQIIVYAGAVMTLFLFVIMLVGVDKEESWVEELPRQRIWVGALAGVV
                     IVFSALVTVVGDFDWVPAAVDTSEETRINGTVQAVAEPLFTDWVLAFEATALLLTIAA
                     AGAVVLAQFKRQRS"
     gene            complement(2571..3230)
                     /gene="nuoI"
                     /locus_tag="VLS86_05145"
     CDS             complement(2571..3230)
                     /gene="nuoI"
                     /locus_tag="VLS86_05145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HSO49910.1"
                     /translation="MGLIRELIKGLRVTGGTMIRTIAGDELVTTQYPKEQRVKPQRFH
                     GRHVLNRYPDGMEKCIGCELCAGACPAQCIYVRGMDNDPEHPTSPGERFGFVYEINLL
                     RCIFCGLCVEACPTEAITMTHLFEISTTNRADAIYTKETLLMDPAGKVPHMFPDEPLA
                     DLGELNAADGWLRATAPSGVASYEGLKMWRDTPGPANWDPEPTQEERDRSTKVRTGSD
                     G"
     gene            complement(3230..4339)
                     /gene="nuoH"
                     /locus_tag="VLS86_05150"
     CDS             complement(3230..4339)
                     /gene="nuoH"
                     /locus_tag="VLS86_05150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HSO49911.1"
                     /translation="MSWPEFWIVLLRVVLTFVLLMVVTILNVWAERKILADMQSRIGP
                     NRAGPFGILQTVADGLKLFFKESILPRKAELGMYLLAPFLAVIPAFLIFMSVPWGKPF
                     MIGEYEVALQGADLNVGLLFILAMSSLAVYAVVLAGWSSGSKYPLIGGVRATAQAISY
                     EAAMGLAMVSVVVFSATQGNGGTLSLAEIVDRQAGTWGNLIPQLEPVLRFVPRWNIFP
                     QFVAFVIFFIAIVAETNRSPFDLVEAEQEIVGGFHTEYSGMRFAMFFLAEYVNIFSMS
                     AIAATLFLGGWNGPTFDDALPGFISAILPTVYFLAKTYFIIFLFFWIRATLPRLRYDQ
                     LMQLGWKRLIPGALGWLVISTFVISFRQFGLPWRN"
     gene            complement(4336..>4616)
                     /locus_tag="VLS86_05155"
     CDS             complement(4336..>4616)
                     /locus_tag="VLS86_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HSO49912.1"
                     /translation="RTLYDDGVMMRHTPMLAPLAPGAVAHLNPNDAKTVGALAGFRLR
                     VVTKQGEGELVAVIDEGTPAGVVYVPFNQPDGVSLGTDAVVRVTTVGR"
BASE COUNT          951 a         1503 c         1495 g          667 t
ORIGIN      
        1 caccgcgccg tcgaccagcg gcaggtcgat gtcgaatgcg gcaacttcgg ccgcctttcg
       61 gcccggcgcc accaggaccc tcccgtagag atcgtcgacc ccgtatgcct gctcccaggt
      121 gccccacagc ggttcgaagg actcctcgaa ccgacgccac ccctccttgg gccgtcgata
      181 ggtgagaaag ccgactccga tgccgaccac ggccgcgagc acggacaacc ctgcgagcaa
      241 ggcgaacatc agccagccct cgtcggggtg ctggacatgg accaactcga agctcggctc
      301 caggaaatgt tcgagcgagg tgcgaaacgg agtgttgacc agacccccta ccaccgacag
      361 ggccgccaac acgaccaggg gaagcgtcat caccctcggg gactcgtggg gatgggccag
      421 gtcgtcccag cgtggctcgc cgagaaagac cagaacccac tgacggctca tgtagaacgc
      481 ggtgaggaga gccgtcagca agcccacgat ccacagcgcc gagaaccagc cgccacgggc
      541 gaacgaagag gcgaggatct cgtccttgga ccagaacccg gccaaggggg ggatgccggc
      601 gatggcgagg gtggcgatgc ccatcgtgat cgcggtgacc ggcatcttct tgaacagccc
      661 acccatcttg tgcatgtcct gctcgtcgtg catggcatgt attaccgagc cggcgccgag
      721 aaagagaagg gccttgaaga aggcgtgggt catcagatgg aagatcccgg cgacatatgc
      781 ggtggcgccc accgcgagga acatgtagcc gagctgggag atcgtggagt aggccagcac
      841 ccgcttgatg tcccgctggg ccatggcgat cgacgcagcc cagagagcgg tgatcgcccc
      901 caccgtggcc accaccgtcg aagcaagagg cgtgagctcg tagatggctg cggagcgggc
      961 gatgacgtac acacccgcag tgaccatggt cgcggcatgg atcaaggccg acaccggggt
     1021 agggccctcc atggcgtcgg gcagccagac gtagagaggg atctgcgccg acttgcccgc
     1081 ggccccgacc aggatcaaca acccgatggc ggttgccatg ccgctggtca gctccgcgcc
     1141 ggcccgctcg aagatgccgg agtaggacag ggtgccaaag gtcgcgaaaa ccagcatcaa
     1201 gccgaccatg aacccgaagt cgccgatccg gttcacgatg aacgccttct tggcggctgc
     1261 ggccgcggtg ggtctggtga accagaaccc gatcaggaga taagagcaga gcccgaccaa
     1321 ctcccacccc aggaagagca tggcgtagtt gccggcgagg accagggtga gcatcgatgc
     1381 ggcaaacagg ttgagatagg tgaagaaccg ggagaagcgc gggtcaccgt gcatgtagcc
     1441 gatggcaaag acgtggatca gcgcccccac cccggtgacc accagggcca tcagggcggc
     1501 gagcgggtcc cacagcagct cgaggttggc cccgatcgcc ggcatccact cccacaacga
     1561 cacgatcgtc cccgactccc cgccctcgaa gaaggggatg gcggcgacag cggcgatcgc
     1621 gaacgaaccg ccgatcgcgg cggtggccac ccagccggca agaggctccc tgagatagcg
     1681 gccaaggaaa tggaggaaca gcgccccacc cagggggagt gcgatgacca gccagatgaa
     1741 gtcggtcacc actagccccg cagctcgttc agctcgtcga ccgaggcaga ggtccgtcgc
     1801 cggaagatgg cgacgatgat cgccagaccg accaccacct ctgctgcagc cacgacgagg
     1861 atgaagaaca cggcgacctg gccgttgatg tcgttcatgg cacgccccgc cgagatcagg
     1921 gtgaggttga ccgcgttgag catcaactcg atgcacatga acatcaccag ggcgttgcgt
     1981 cggagcagca tccccagtgc cccgatcagg aacagggcag cagccagagc catgtaccag
     2041 tcgacggaga ccactacgac ctctgcctct tgaactgggc gaggaccacg gcgccggcgg
     2101 cggcgatggt caggaggagg gcggtggcct cgaaggcaag cacccagtcg gtgaacagcg
     2161 gctcggcgac cgcctgaacc gtcccattga tccgggtctc ctcactggtg tcgaccgcgg
     2221 ccgggaccca gtcgaaatca ccgaccacgg tgacgagagc gctgaacacg atcacgaccc
     2281 cggccagggc cccgacccat atccgctgtc tcgggagctc ctcgacccaa ctctcttcct
     2341 tgtccacccc gaccagcatg atcacgaaca ggaacagggt catcaccgcc ccggcgtaga
     2401 cgatgatctg caccgcagcc accagatggg ccagttggac cacgtagagg accgccaccg
     2461 agaacagcgt tcccaacagg cccatcgccg agaagaccgg gttgcgggcg atcaccacgg
     2521 tcactgctcc ggccagggcg gtgatcccca tgagcacgaa gagaatcgcc tcagccatcg
     2581 ctgcccgttc tcaccttcgt gctgcgatcg cgctcttcct gagtgggctc cggatcccaa
     2641 ttggccgggc ccggcgtgtc ccgccacatc ttcagccctt cgtagctcgc aaccccggaa
     2701 ggcgccgttg cccgcaacca gccgtctgcg gcgttgagct ctccgaggtc ggccagcggc
     2761 tcgtcgggga acatgtgtgg caccttcccg gccggatcca tcagcaacgt ctccttggtg
     2821 tagatggcgt cggcccggtt ggtggtcgag atctcgaaga gatgggtcat ggttatcgcc
     2881 tcggtggggc aagcctcgac gcacagcccg cagaagatgc agcgcaggag attgatctcg
     2941 tagacgaatc cgaatcgctc ccccggggag gtgggatgct cgggatcgtt gtccatgccc
     3001 cggacgtata tgcactgggc ggggcaggct ccggcgcaga gctcacagcc gatgcacttc
     3061 tccatgccgt ccgggtagcg gttgagcacg tgacgcccgt ggaagcgctg gggcttgacc
     3121 cgctgctcct tcgggtactg ggtggtgacc agctcgtccc cggcgatggt gcggatcatg
     3181 gtcccaccgg tcacccggag ccctttgatc agttctcgga tgagacccat cagtttctcc
     3241 aggggagtcc gaattggcgg aaggagatga cgaaggtgga gatcaccagc caaccgagcg
     3301 cacccgggat cagacgcttc cacccgagct gcatcagctg gtcgtagcga agccggggaa
     3361 gcgtcgccct gatccagaag aagaggaaga tgatgaagta ggtcttggcc aggaagtaga
     3421 cggtgggcag gatcgccgag atgaacccgg gaagggcgtc gtcgaaggtg gggccgttcc
     3481 aaccaccgag gaacagcgtt gccgctatcg ccgacatcga gaagatgttc acgtactcgg
     3541 cgaggaagaa catggcgaac ctcatccccg agtactcggt gtggaacccg ccgacgatct
     3601 cctgctccgc ctcgaccagg tcgaatggtg accggttggt ctctgccacg atggcgatga
     3661 agaagatgac gaaggcgacg aactggggaa agatgttcca tctgggcacg aagcggagca
     3721 ccggctccag ctgcgggatc aggttccccc aggtgcccgc ctgacgatcc acgatctcgg
     3781 ccagggacaa cgtcccacca ttgccctggg tcgcggagaa gaccacgacc gagaccatgg
     3841 ccagccccat cgccgcctcg taggagatcg cctgagcggt tgccctgacc ccgccgatca
     3901 gcgggtactt ggagcccgac gaccacccgg cgaggaccac cgcatacacc gccagcgagg
     3961 acatggcgag gatgaacaac aggccgacgt tgaggtcggc gccctggagc gccacctcgt
     4021 actcgccgat catgaacggc ttgccccacg ggaccgacat gaagatgaga aaggcgggga
     4081 tcacggcgag gaagggcgcg agcaggtaca tccccagctc cgccttgcgg gggaggatcg
     4141 actccttgaa gaacagcttg aggccgtcag ccaccgtctg caggattccg aaaggcccgg
     4201 cccggttggg gccgatccgg ctctgcatgt cggcgaggat cttccgctcc gcccagacgt
     4261 tgaggatggt caccaccatg agcaggacga aggtgagcac cacccggagc aacacgatcc
     4321 agaactcggg ccagctcatc tgcccaccgt cgtcacccgg accacggcat cggtcccgag
     4381 cgacacaccg tctggctggt tgaagggcac gtagacgaca cccgccgggg tcccctcatc
     4441 gatgacggcc accagctcgc cctcaccctg cttggtcacc accctcaacc ggaacccggc
     4501 cagggcgccg actgtcttgg catcgttggg attgagatgg gccacggcgc cgggtgccag
     4561 cggggccagc atcggggtgt gccgcatcat gaccccgtcg tcgtagaggg tcctgg
//
LOCUS       DATITW010000363         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_539377, whole genome shotgun sequence.
ACCESSION   DATITW010000363 DATITW010000000
VERSION     DATITW010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539377"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1058
                     /locus_tag="VLS86_05160"
     CDS             171..1058
                     /locus_tag="VLS86_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSO49913.1"
                     /translation="MSATASTTVVTQRPNTPPNPRLKRWLRRIGIGIPLLVLAVWTIY
                     PVLMTLSISFKTRADVSADPGLIPSNPTLDGYRSVLERQGFQDAFFNSMVVGLGTALL
                     TVLLAVPAAYAFARFRFRGRHLLLLFILLPRLIPGLGLMVPLYRLAVALGAIDQLLTL
                     IVAFSATVVPLAVWLFVGFFQQVPRELEEAANVDGASLFTRLRYIVLPLAFPAIITIG
                     VLAFREAWNEFNLVLGLTSTPGSRTLPYELFLMTQTTGFQDNPGQAAFAIMTIIPFIF
                     VYLRLERYVVSGLTSGALK"
     gene            1152..2447
                     /locus_tag="VLS86_05165"
     CDS             1152..2447
                     /locus_tag="VLS86_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSO49914.1"
                     /translation="MDRQRRSHLIAIMVISMLVLAACNGGAGDETTTSAAAGGDGEGV
                     QVVAWFNGETVPADEFASLEADGVSASYDIRGDAVLTDMLRMRDAGEQLPDLVEIDSH
                     LTPAFMEAGLLAPMTEQVATWEEEDPELYATVPESVWEDGTYDGDIYHFPNKSLVDAL
                     FYNVAMLEEAGATPPPYETYWEMLDAARAVQAANPDLAAYFATGGASHDRMFRWLYAF
                     GVPFEGNIPDVNTEQGIEMIDWLQTMFEEGIVDPEFMINQQDEGKGAFVAGELPFIEE
                     GLNGGGGFMLDDYQYGDDWLTAPVPVHEENGGVYMGVPRGWSMAVDAANPYEASLVLR
                     YLSEPEIAAERYFDLESGVVQSIPVLEGEQMAEAQPFFTAELLEIFTTMEAQIPPGTN
                     TNAVGEVLVNMVEELTVTGTDESPEEVAARYQPMFDELG"
BASE COUNT          440 a          823 c          815 g          466 t
ORIGIN      
        1 ggtgggccgg tcgacgccac gcggacgatg ccgatagcgc tgtacgaggc ggcttttgtc
       61 gatctggaga ccaacaaggc actcgccatc gtcgtcgtca tcctggtctt caacgcgttt
      121 ctcacactcg gctactgggg cttgtcgaga cgttacgacc tggaggccac atgagcgcca
      181 cggcgtcgac caccgtcgtc acccagcgcc ccaacacccc tcccaacccg cgcctgaagc
      241 gttggctgcg caggatcggg atcgggattc cgctcctcgt cctcgccgtg tggaccatct
      301 acccggtctt gatgacgttg tcgatctcgt tcaagacccg tgccgatgtg tcggccgatc
      361 ccggtctcat cccgtcgaat cccaccctcg acggctaccg ctctgtcttg gagcgccaag
      421 gtttccagga cgcattcttc aacagcatgg tcgtggggtt ggggacggcg ctgctcaccg
      481 tgctgctcgc cgtccctgcc gcctacgcct tcgcccggtt ccgcttccgg ggccgccacc
      541 tgctgctgct cttcatcctg ctgccccggc tgatccccgg tcttggcctg atggtccccc
      601 tgtatcggct cgccgttgcc ctgggagcca tcgaccagct cctgactctg atcgtggcct
      661 tcagcgccac ggtggtgccg ctcgcggttt ggctgttcgt gggcttcttc cagcaggtgc
      721 cacgtgagct ggaagaagcg gccaacgtcg acggggcgtc attgttcacg cggctccgct
      781 acatcgtgct gccgctggcc ttcccggcca tcatcaccat tggtgtgctc gcctttcgcg
      841 aggcgtggaa cgagttcaac ctcgtgctcg ggctcacctc gaccccgggc agccggaccc
      901 tgccctacga gctgttcttg atgacccaga ccaccggctt ccaggacaac cccggccagg
      961 ccgcctttgc gatcatgacc atcatcccgt tcatcttcgt gtacctgcgt ctggagaggt
     1021 atgtggtgag cggactcacc agcggagccc tgaagtgagc acataaacgc cgtcgcggcc
     1081 acgcggggcc gcacgtggga aggacagaca cgactccgag gaggaggaaa atgggacgaa
     1141 gagcacaacg aatggaccgc cagaggcgaa gtcacctgat cgcgatcatg gtgatcagca
     1201 tgctggtgct ggccgcctgc aacggcggcg ccggcgatga gaccacaaca tctgcggcgg
     1261 cgggaggcga tggggaaggc gtccaggtgg tggcctggtt caacggtgag accgtgccgg
     1321 ccgatgaatt cgcttcgctc gaggcggacg gagtcagcgc ctcctacgac atccgcgggg
     1381 acgccgtcct caccgacatg ctgcggatgc gtgatgccgg cgagcagttg ccggacctgg
     1441 tcgagatcga ctcgcatctg accccggcgt tcatggaggc ggggctgctg gcaccaatga
     1501 ccgaacaggt ggcaacgtgg gaggaggaag accccgagct ctacgcgacc gttccggagt
     1561 cggtttggga agacgggacc tacgacgggg acatctacca cttccccaac aagagcctgg
     1621 tcgacgccct gttctacaac gtcgcgatgc tcgaggaagc cggcgctacg ccacctccct
     1681 atgagaccta ctgggagatg ctcgatgcgg cccgagccgt ccaggccgcc aacccggacc
     1741 tggccgccta cttcgcgacc ggtggcgcca gccatgaccg catgttccgg tggctctatg
     1801 cctttggggt gcctttcgag ggcaacatcc cggacgtgaa caccgagcag ggaatcgaga
     1861 tgatcgactg gctccagacg atgttcgagg aaggcatcgt cgaccccgag ttcatgatca
     1921 atcagcagga cgaaggcaaa ggcgccttcg tcgccgggga gctgccattc atcgaggaag
     1981 gcctcaatgg tggcggtggg ttcatgctcg acgactacca gtacggcgac gactggctga
     2041 ccgcgcctgt gcccgtccac gaggagaacg gtggtgtgta catgggcgtg ccccgtgggt
     2101 ggtcgatggc tgtcgacgcc gcgaacccct atgaggccag cctggtgctc cggtacctgt
     2161 ccgagcccga gatcgctgcc gagcggtact tcgacctcga atccggagtg gtccagtcga
     2221 tcccggtctt ggaaggcgag cagatggccg aggcccagcc cttcttcact gccgagctgc
     2281 tcgagatctt caccactatg gaggcgcaga taccgcccgg cacgaacacc aatgcggtcg
     2341 gagaggtgtt ggtgaacatg gtggaagagc tgacggtgac cggcaccgac gaatcaccgg
     2401 aggaggtggc cgcccggtac cagccgatgt tcgacgagct gggctagctc cagctgtagc
     2461 tctgctgacg agagcctccg ggtccgggcg cgatacgccc gaaccgaaag ggaggctctc
     2521 gtcgttagtg gcatcgcggc attt
//
LOCUS       DATITW010000364         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_338788, whole genome shotgun sequence.
ACCESSION   DATITW010000364 DATITW010000000
VERSION     DATITW010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338788"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="VLS86_05170"
     CDS             complement(<1..1596)
                     /locus_tag="VLS86_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HSO49915.1"
                     /translation="MSEYQPPLDDMTFSLNHVAGLESISKLNGYQHADPETVGTILEE
                     AGRFFSEVIAPLNRIGDQQGSVLTEDGVVRTPDGFKEAYQKYVDSGWASAHLPEEWGG
                     GGLPYIVGVAIQEMFKTANMAFALAPTLTHGAVEALQRHGSEELKTRYLEKLVSGEWT
                     GTMNLTEPEAGSDVGALRTRAVKHDDGSYRLFGTKIFITWGDQDLTSNIIHLVLARTP
                     ESPPGTRGISMFLVPKFIVDENGEPGERNALKVLSLEHKLGIHASPTCVVSYGDGGEG
                     AVGYLVGDEREGMRNMFTMMNASRVGVGMEGVAIGERAYQHALHYARERVQGRPVGAD
                     ARESVPIIEHPDVRRMLLTMKAYVEAMRSLLYLTAGEADHMVHADTEERRRLASDRLA
                     LLTPIVKAWCTDVGVELASIGVQIHGGMGYVEETGAAQYLRDSRIAPIYEGTNGIQAI
                     DLVLRKVPLENGAVVAGLLSEIGLVRDRLGQHQELARFRDELSAALQGLIESSTWLGE
                     RLGAGDVGSALAGASPYLRQFGTV"
     gene            complement(1715..>2232)
                     /locus_tag="VLS86_05175"
     CDS             complement(1715..>2232)
                     /locus_tag="VLS86_05175"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HSO49916.1"
                     /translation="HEAIKRLADSGRLSGVVTQNVDGLHHQSGLDDHLVAELHGNVRQ
                     SLCVDCDRTWATDEVLAWVEAGDLDPSCPECGGIVKTGTVMFGELLPEQEMQKAMLFL
                     AAADAIIVVGSTVAVWPASDVVMRGAFRSLPIVIINQGETEADHLAAVKLDAGIGAVL
                     PEIVNGLLDAR"
BASE COUNT          368 a          736 c          732 g          396 t
ORIGIN      
        1 gacggtcccg aactggcgaa ggtaggggga ggccccggcc agcgccgagc cgacgtcccc
       61 ggcgccgagt cgctccccga gccacgtgga gctttcgatc aggccctgga gggcggcaga
      121 tagttcatcc cggaaccggg ccaactcctg atgctggccc aggcgatctc tgaccaggcc
      181 gatctcggac agcaggccgg ccacgacagc gccgttctcg aggggaacct tgcgcaggac
      241 gaggtcgatc gcctggatgc cattggtgcc ttcgtagatg ggtgcgattc tcgagtcgcg
      301 cagatactgg gcggcgccgg tttcctccac gtatcccatg ccaccgtgga tctggacccc
      361 gatcgatgcc agctcgaccc cgacatcggt gcaccaggcc ttgacgatgg gggtgagcag
      421 ggcgagacgg tcggaggcga gccgtcggcg ctcctcggtg tcggcgtgga ccatgtggtc
      481 ggcctcgccc gcggtcagat agaggagcga cctcatggct tcgacgtagg ccttcatggt
      541 gagcagcatc cggcgcacgt cggggtgctc gatgatggga acgctctctc tggcgtcggc
      601 gccgacggga cggccctgga cccgttccct ggcgtaatgg agggcatgct gatatgcccg
      661 ctcgccgatg gcgacacctt ccattccgac cccgacgcgg gaggcgttca tcatggtgaa
      721 catgttccgc atcccctcgc gttcgtcacc caccagatat cccaccgcgc cctcgccgcc
      781 gtcgccgtaa gagacgacac aggtcggcga ggcgtgaatc cccagcttgt gctctaagga
      841 gagcaccttg agagcgttgc gctctccggg ctcgccgttc tcgtcgacga tgaatttcgg
      901 caccaggaac atcgagatcc cgcgggtccc cggaggtgac tcgggggtcc gggccagaac
      961 caggtggatg atgttggagg tcaggtcctg gtcgccccag gtgatgaaga tcttggtccc
     1021 gaacagccgg taactgccgt cgtcatgttt caccgccctg gtcctcaggg cgccgacgtc
     1081 tgatccggcc tcgggctcgg tgaggttcat ggtgccggtc cactccccgc tcaccagctt
     1141 ttccaggtac cgggtcttga gctcctcgga gccgtggcgc tgcagcgcct ccactgcgcc
     1201 gtgggtgagg gtgggggcca gggcgaaggc catgtttgcg gtcttgaaca tctcctggat
     1261 ggcgacaccg acgatgtagg gcaagccccc tccgccccac tcctcgggca ggtgcgccga
     1321 ggcccaaccc gagtcgacgt acttctggta ggcctctttg aacccgtcag gcgttcggac
     1381 cacaccgtcc tcggtcagca ccgagccctg ctggtcgccg attcggttga ggggggcgat
     1441 cacctcggag aagaaacgcc ccgcctcctc caagatggtg cccacggtct cggggtcggc
     1501 gtgttgatat ccgttgagct tggagatgga ctccagtccg gccacatggt tcagactgaa
     1561 cgtcatgtcg tccagaggtg gctggtactc ggacatggct caaggctacc cccggccccc
     1621 gccttccgtg attgggaccc acaggtccgg atatgacccc tctcggtccc aatcacggag
     1681 gggccactaa gagggggcga ccacgcaggg gcggtcagcg ggcgtcgagc agaccgttca
     1741 cgatctcggg gaggacggcc ccgatcccgg cgtcgagctt caccgcggcc aggtggtcgg
     1801 cttcagtctc cccctgattg atgatcacga tgggcaacga acggaaggca ccgcgcatga
     1861 ccacgtcgga ggccggccac acggccacgg tggatccgac cacgatgatg gcgtcggcgg
     1921 cggcgagaaa cagcatggcc ttctgcatct cttgctccgg gagaagctcc ccgaacatga
     1981 cggtgccggt cttcacgatc ccgccgcact cggggcagga agggtcgagg tcacccgctt
     2041 ccacccaggc gagcacctcg tcggtggccc aggtccggtc acagtcgaca caaagactct
     2101 gtctcacgtt cccgtgcagc tctgccacca ggtggtcgtc gagacccgat tggtggtgaa
     2161 gcccgtcgac attctgggtg accactcccg aaagtcttcc gctgtcggcg agacgcttga
     2221 tcgcctcatg cc
//
LOCUS       DATITW010000365         3003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_18622, whole genome shotgun sequence.
ACCESSION   DATITW010000365 DATITW010000000
VERSION     DATITW010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18622"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /locus_tag="VLS86_05180"
     CDS             <1..1538
                     /locus_tag="VLS86_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSO49917.1"
                     /translation="AVPARRLRYRQNGATQGSNREEIPMSKRWLIAMIAALALIVAAC
                     GGDTATTTTEATGTTEAPGTTAGEETPTTTGEAPGESTGYEHLDQALAGDFEGTTVDI
                     WAQWLPESSEAANFTYTLEPFQEATGITVNFQPTPDYETALQVAVDGGNAPDLAQIAQ
                     PGKMHQYADAGSLVGLSDWLNTEKLSADMVPAFYELGTYNDTLYGVYYKADVKSIVWY
                     PVQAFADAGYEVPTTWDELVALSDQIIADGNGNPWCISIESQEADGWVATDWMEDILL
                     RTAPPETYNQWITHEIPFNDPEVLEAADYMSQIWFTPDYAYGGNTYINATWIGEAQDP
                     MFNPDGPDCWMQKQAAWIGGFWGANRDALADDPNLPASEWPNKPETDVDFFYFPQIEE
                     EFGTPVLGAADMFVMFNDRPEVRALLEWLGTPEAAEGWIERGGFLSTLTTVPEDWYVY
                     PNNQLAPMVAEATTLGFDASDLMPAEVGVGSFWSGMVDWVAANGEGTEEIFQSIEDSW
                     PTG"
     gene            1588..2748
                     /locus_tag="VLS86_05185"
     CDS             1588..2748
                     /locus_tag="VLS86_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSO49918.1"
                     /translation="MGGPLAEAIQPIRPTPPFLLILRALLVIGGVAAALWLGLGVLRD
                     PDVFSGLGPSLSKLLQALFAVALGVGGIWALFYAGNVAIEALPTRATQFLRPYLFVLP
                     AMAVVGLYLIYPTVGTVVQSFTEVPEGASPFTNYSNAFTNNEVLLALRNNLIWLLIAP
                     AASVLIGLAFAALVDRVRRESLAKSFVFLPLAVSFVGASVIWRFMYAWKPPGQPQIGV
                     LNAIWVGVGGFLVDLGLLTEVEPVAWLQIIPWNTLALIAIMVWLQVGLAMVILSAAIK
                     GVPPELTEAARIDGASELQAFFRITVPSIRGSILTVFTTIAIAVLKVFDIIFVTTGGN
                     FDTSVVAVRMYQEMFRFRNFGQATTMAVILLIAVLPIMVVNIRNLRRQGVGA"
     gene            2745..>3003
                     /locus_tag="VLS86_05190"
     CDS             2745..>3003
                     /locus_tag="VLS86_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49919.1"
                     /translation="MTTTTRKPRAPSHHRAGWLVKLAVFFIVAVWTIPSLGLLTNSFR
                     TSVDSSTTGWWTSLLNPVSSEWTLDNYNRVLTSGGLFDALIN"
BASE COUNT          556 a          973 c          925 g          549 t
ORIGIN      
        1 aagcagtccc cgctcgccgt ttgcgatacc ggcagaacgg ggcgacccaa ggaagcaacc
       61 gggaggagat ccccatgagt aaacgatggc taatcgcaat gatcgcggcc ctggccctga
      121 tcgttgcagc ctgcggtggc gacaccgcta ccaccaccac ggaggcgacg ggcaccaccg
      181 aggctccagg cacgacagcg ggagaggaga ccccgaccac caccggtgaa gcgccgggag
      241 agtcgacagg ctacgaacat ctcgaccagg cattggcggg agacttcgaa gggaccaccg
      301 tggacatctg ggcgcagtgg ctgcccgagt cctccgaagc cgccaacttc acctacacac
      361 tggagccgtt ccaggaagcg accgggatca cggtcaactt ccagccgact cccgactacg
      421 agaccgcgtt gcaggtggcg gtcgatggcg gcaacgcccc cgacctggct cagatcgcgc
      481 agcccgggaa gatgcaccag tacgccgatg ccggctcgct ggtgggtctg agcgactggc
      541 tcaacaccga gaagctgtcc gccgacatgg tgccggcctt ctacgagctg ggcacctaca
      601 acgacacgct gtacggcgtg tactacaagg ccgacgtgaa gtcgatcgtc tggtacccgg
      661 ttcaggcctt cgccgacgcc ggatacgagg ttccgaccac gtgggatgag ctggtggctc
      721 tcagcgacca gatcatcgcc gacggcaacg gcaacccgtg gtgcatctcg atcgaaagcc
      781 aggaggccga cggctgggtg gccaccgact ggatggagga catcctcctt cgcacagctc
      841 caccggagac ctacaaccag tggatcacac acgagatccc gttcaacgat cccgaggtgt
      901 tggaagcggc cgactacatg tcgcagatct ggttcacgcc ggactacgcc tatggcggca
      961 acacctacat caacgcgacc tggatcggtg aggcgcagga tccaatgttc aacccggacg
     1021 gccccgactg ctggatgcag aagcaggccg cctggatcgg cggattctgg ggagctaacc
     1081 gtgacgcgtt ggccgatgac ccgaacctcc cggccagcga atggccgaac aagcccgaga
     1141 ccgatgtcga cttcttctac ttcccgcaga tagaagagga gttcggcaca ccggtgctcg
     1201 gcgcggccga catgttcgtg atgttcaacg atcggccgga agtccgggca ctcctcgagt
     1261 ggctgggaac tcccgaagct gccgaagggt ggatcgagcg aggaggtttc ctctcgactc
     1321 tgacgactgt ccccgaggac tggtacgtct accccaacaa ccagctggcg ccgatggtcg
     1381 ccgaagcgac gacgctcggc ttcgacgctt ctgacctgat gcccgccgag gtgggggtcg
     1441 ggtccttctg gagcggaatg gtcgattggg tggcagccaa tggcgaaggc accgaggaga
     1501 tcttccaatc gatagaagac agctggccca ccggctgaga aagacggtga ttagggtccc
     1561 ggaaccacgt tccgggaccc ttttcacgtg ggaggaccct tggcagaggc aatccaaccg
     1621 atcaggccaa ccccgccttt tctcctgatc ctccgtgctc ttctggtgat cgggggggtg
     1681 gctgcggcac tctggctcgg tctcggagtg ctgcgagatc ccgacgtgtt ctccggtctc
     1741 gggcccagct tgtccaaact gctgcaagcg ctcttcgccg tcgccctcgg agtggggggc
     1801 atctgggcgc tcttctacgc aggcaatgtc gccatcgagg ccctgcccac ccgagctacc
     1861 cagttccttc gcccctatct cttcgtgctg ccggcgatgg cggtggtggg gctctacctc
     1921 atctacccga cggtcgggac cgttgtgcag agcttcaccg aggtgcccga aggggcaagc
     1981 ccgttcacca actacagcaa tgcgttcacc aacaacgaag tgctgctggc gttgcgcaac
     2041 aacctgatct ggttgctgat cgcacccgcg gcgtcggtgt tgatcggtct cgcctttgcc
     2101 gctctcgtcg atcgggtgcg tcgtgagtcg ctggccaagt cattcgtctt cctgccgctc
     2161 gccgtgtcgt tcgtgggcgc ctcggtcatc tggaggttca tgtacgcctg gaagcccccg
     2221 ggccaacccc agatcggggt tctcaacgcc atctgggtcg gggttggtgg cttcctcgtc
     2281 gacctcggcc ttctgaccga ggtcgagccg gtggcctggc tgcagataat cccctggaac
     2341 actctggccc tgatcgccat catggtctgg cttcaggtgg gtctggccat ggtgatcctg
     2401 tccgccgcga taaagggcgt ccctcccgag ttgaccgaag cggcgcgcat cgacggggcg
     2461 tccgagctgc aggcgttctt ccggataacc gtgccttcga tcagaggctc gatcctcacc
     2521 gtgttcacca cgatcgccat tgcagtgctc aaagtcttcg acatcatctt cgtcaccaca
     2581 ggagggaact tcgacacctc ggtggtggcg gtccggatgt atcaggagat gttcaggttc
     2641 cgcaatttcg ggcaggcgac cacgatggcg gtgatcctgc tcatagcggt gctcccgatc
     2701 atggtggtca acatccgcaa tctccgccgc cagggagtcg gagcatgacc acgacgacca
     2761 gaaagccgag agcgccgagc caccaccgag ccggctggct ggtcaagctc gccgtcttct
     2821 tcatcgtcgc ggtgtggacc attccctcgc tcgggctgct caccaactcg ttccgcacct
     2881 ctgtcgacag cagcacgacc ggttggtgga cctctctcct caacccggtg tcatcggaat
     2941 ggaccctcga caactacaac cgggtcctga catccggtgg cctgttcgac gccctgatca
     3001 aca
//
LOCUS       DATITW010000366         2728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_579611, whole genome shotgun sequence.
ACCESSION   DATITW010000366 DATITW010000000
VERSION     DATITW010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579611"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /gene="pknB"
                     /locus_tag="VLS86_05195"
     CDS             complement(<1..1907)
                     /gene="pknB"
                     /locus_tag="VLS86_05195"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HSO49920.1"
                     /translation="MDSKTLTDRYQITSHLARGGMADVYEGMDTLLNRRVAIKVLHSQ
                     YSADEAFVKRFRREAQAAANLSHPNIVGIYDWGQAQNTYFIVMELVDGRSLRDVLKSE
                     GALLPRRATEITAEVAAALSVAHQAGLVHRDVKPGNILLAKDGTVKVTDFGIARAWDD
                     SQELTRTGAVIGTATYFSPEQAQGASADARSDVYALGVVLYEMLTGRPPYTGDSPMSV
                     AFQHVSTEAAMPSSLNSDGPPSLDAVVAKAMRKDPTARYQTADEMRADLLSVLRGEPV
                     GMPVVVPVPSNGENSTRLMTTTVPPATVPPDEVYRGVEEEPRSQLPFILTAFGLLIAL
                     GILLFILFRFAGGGGDGDLVEVPNVTGLSQDEALQRLQDDGFVPRLFFEPSDTVEVGN
                     VIRTDPTFGTEAEEGSTVDVYVSSGAEEIAVPPLVGETLDDARQLLEDAGLTLGTVTD
                     RPDADRPEGTVIEQSPAAGVEVGNGAPVNVVVSTGPELVILPDLTNFSERDAVAELNR
                     LGLLFTFDEEFSDEVAEDDVVRTDPPANTEMQSGDTVLLVISQGPEPVEVPNLTGLTP
                     GQAEDILEDLGLELNVSNATQPVADPSQDGRVVSQSPGVGSTVIPGDIVTVTLGEFEP
                     PPPTPPTTTTPP"
     gene            complement(1946..>2728)
                     /locus_tag="VLS86_05200"
     CDS             complement(1946..>2728)
                     /locus_tag="VLS86_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HSO49921.1"
                     /translation="IDTGTALPETVFPDPEEFDLPESDATISNAGGGVCNDGESITLL
                     QAFVVSCNTVFADLSLRLGAEDIGLTAEGLGFNTDIPFPWTVPEAIYPVDLLAADPAA
                     LAQSGIGERDVRAAPLHMAMVAAAVANDGATPTPFLVNRIFDADGTTVETFEPAVMGQ
                     AMDSATAAIVADMMEQVVTDGTGRGASVAGVRVAGKTGTSMGPDGLPNAWFIGFAPVE
                     DPTIALAVFFQASPELGESATGGGVAAPLAGELIELWLEGTP"
BASE COUNT          443 a          881 c          950 g          454 t
ORIGIN      
        1 ggcggggtag tagtagtggg gggggtcggc ggcggtggct cgaactcgcc cagtgtcacc
       61 gtgacgatgt caccaggaat cacagtggac ccaactccgg ggctctggga caccacccga
      121 ccgtcctggc tggggtcggc cacgggttgg gtggcgttgg acacgttcag ctccagaccg
      181 aggtcctcga ggatgtcctc ggcctgaccc ggggtgagcc cggtcaggtt cgggacctcc
      241 accggctcgg ggccttggga gatgacaagc agaacggtgt cgcccgactg catctcggtg
      301 ttggccggcg ggtcggtgcg gaccacgtcg tcctcggcga cttcgtctga gaactcctcg
      361 tcgaaggtga acaacaggcc gagccggttg agctcggcga ccgcgtctcg ctcgctgaaa
      421 ttggtcaggt cggggagaat caccagctcg ggcccggtgg acaccaccac gttcaccggc
      481 gccccgttcc ccacttcgac accggcggca ggtgactgct cgatgaccgt gccctcgggg
      541 cggtcggcgt cgggtcggtc ggtcaccgtg cccaatgtga gcccggcatc ctcgagcagt
      601 tgccgggcgt cgtccagggt ctcgccgacc aaaggcggca ccgcgatttc ttcggcaccc
      661 gaggagacat agacgtcgac cgtggagccc tcctccgcct cggtcccgaa tgtcgggtcg
      721 gtgcgaatca cattgccgac ttcgacggtg tcgctgggct cgaagaacag cctcggcacg
      781 aaaccgtcgt cctgaagccg ctgaagtgcc tcgtcctggc tgagaccggt gacgttcggc
      841 acctcgacga gatcgccgtc tccgcctcca ccggcgaatc ggaacaggat gaacaagagg
      901 atgccgaggg cgatgagcag cccgaaagcg gtgaggatga acggcagctg ggaccggggc
      961 tcttcctcca cgccccggta cacctcgtcg gggggcaccg tcgccggggg gacggtggtg
     1021 gtcatcaacc tcgtcgagtt ctcgccgttg gacgggacgg gaaccactac cggcatccct
     1081 accggctcgc ccctgagcac cgacagcagg tcggcccgca tctcgtcggc agtctggtag
     1141 cgggcggtgg gatcctttct catcgccttg gccaccaccg catcgagcga gggcggaccg
     1201 tcggagttga gcgaactcgg catcgcagcc tcggtggaga catgctggaa ggcgaccgac
     1261 atcgggctgt ccccggtgta cggcgggcgc ccggtgagca tctcgtagag cacgaccccg
     1321 agggcgtaga cgtcgcttcg ggcgtcggcg gatgcaccct gggcctgctc cgggctgaaa
     1381 taggttgcgg tgccgatgac cgccccggtc cgggtgagct cctggctgtc gtcccaggcc
     1441 cgggcgatgc caaagtccgt gaccttcacc gtgccgtcct tggcgaggag gatgttgccg
     1501 ggcttgacgt cccggtgcac cagccctgcc tgatgggcga cggacagcgc ggcggctacc
     1561 tcggccgtga tctcagtggc acggcgcggg agcagagcgc cctccgactt gagcacgtcg
     1621 cgcagggagc gcccgtcaac gagctccatg acgatgaaat aggtgttctg cgcctggccc
     1681 cagtcgtaga tgccgacgat attggggtgg ctgagattgg ccgcggcttg cgcctcgcgc
     1741 cggaaacgtt tgacgaaggc ctcgtcggcc gagtactggg aatggaggac cttgatcgcc
     1801 acgcggcgat tcaggagcgt gtccattccc tcatagacgt cggccattcc tccccgggcg
     1861 agatgggacg tgatctggta ccggtcggtg agggtcttgg agtccatgga agccgggcgg
     1921 attgtaggac tgctctcgtc ccaactcatg gggttccctc cagccacaac tcgatgagtt
     1981 cgccggcgag cggggcagcg acccctcctc cggtggcaga ttcccccagc tccgggctgg
     2041 cctggaagaa gacggccaaa gcgatcgtcg ggtcctctac cggggcgaag ccgatgaacc
     2101 aggcattggg caggccatcg ggccccatcg atgtcccggt cttccccgct acgcgaacac
     2161 cggcgacgga ggcgccccga ccggtcccat cggtcacgac ttgctccatc atgtcggcca
     2221 cgatcgcggc agtcgccgaa tccatggcct ggcccatcac ggccggctcg aatgtctcca
     2281 ccgtggtccc gtctgcgtcg aagatgcgat tcacgaggaa aggggtgggg gtggcgccat
     2341 cgttggcgac cgcagccgca accatcgcca tgtgcaacgg cgcggcgcgc acatcgcgtt
     2401 ccccgatgcc gctctgggcg agggcggccg ggtctgccgc cagcagatcg accgggtaga
     2461 tcgcctcggg cacggtccac gggaagggga tgtcggtgtt gaaaccgaga ccctcggcgg
     2521 tgagcccgat gtcctcggcg ccgagccgga gcgacaggtc ggcaaacacc gtgttgcagg
     2581 agacgacgaa ggcctggagc aaggtgatcg actccccgtc gttgcagacc ccacccccgg
     2641 cgttggagat ggtcgcgtcc gattcgggca ggtcgaattc ttccgggtcg ggaaagacgg
     2701 tctcgggcag agccgtcccg gtatcgat
//
LOCUS       DATITW010000367         3289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_739495, whole genome shotgun sequence.
ACCESSION   DATITW010000367 DATITW010000000
VERSION     DATITW010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739495"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VLS86_05205"
     CDS             <1..829
                     /locus_tag="VLS86_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSO49922.1"
                     /translation="TTLDQPTAGARSAEEERLIALQKRDTRRRWFWRIAGYVVFLAMW
                     ALTSRIVGENLIPSPSRIIETFPEIVESGRFLSSFAATLTRIGIGFGLSFLIGIALGI
                     FTQSKWLDGFFKDAVTISLVSPGLIWALTGLVIWGFEPQGWIFAIVMTTFALVTVNVA
                     EGIRALPKDLIDMSRAFGVSGWRRQREIVLPFLAPFIFTALRFGFSVGWKVTVLTEVF
                     SSNEGIGFEMRVASQLFRMDELFTWALSFLVFALILEKLVLQRYENKFFRWRQEVTV"
     gene            829..1785
                     /locus_tag="VLS86_05210"
     CDS             829..1785
                     /locus_tag="VLS86_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSO49923.1"
                     /translation="MATMVSGAATRESRFARVLKSPATARLLFPFVVLAVWYLIYYTI
                     DSRIFPTPQEVLEFMWEEVTLTSGVRYSSVAENLYVQFGVSIVRLTIGFFVALILGTV
                     IGLAMGISKSVDAFFHDWVMGVLAMPALVWALFLGLALGFTNVGPIIAVVLAGIPFVI
                     INVREGVRNTPKELFDMARAFDVPQNKITRHVLLPSLLPFFFAAIRYAYAIGWKGLII
                     TEVFASDRGMGWTIKFWYDANRAKGVIGYALFFIIFAMILEKLVFDRLSTKAFRWRPQ
                     INALAMAEGDFGGVTQEGFLLQESQVQEGVPIESMEVESRDG"
     gene            1778..2518
                     /locus_tag="VLS86_05215"
     CDS             1778..2518
                     /locus_tag="VLS86_05215"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO49924.1"
                     /translation="MAEIKVENLHKEFGVGEDRVVALENVNMQVQGHVFVSIVGPSGC
                     GKSTLLNILSGIETPSSGTVTITQDNRDAEPGYVFQAARLLPWRSVIDNLLFVQKDKS
                     DATRERCQKYLEMVQLGDKADKYPGELSGGMQQRVGIARAFSIEPDVLFMDEPFSHLD
                     AITARSLRRELHDMWEQTRKTVLFVTHDVGEAVELSNRILVFAKGGRLEDDLQVELPF
                     PRDPADDQVAVAKAHVLRKFEELDLVVS"
     gene            2572..>3289
                     /locus_tag="VLS86_05220"
     CDS             2572..>3289
                     /locus_tag="VLS86_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49925.1"
                     /translation="MEEKMGKRSRWLVSLVAVLAMVITACGDDTDGEGTDGTADGEAR
                     TVIRFAFAPDPVWDYMNDNGYLVDWEQENNIRIVTSTTWDEFTFFAGGHGDVVSMGTL
                     EIPVLEQETEIQTVTFGKYNALRDSMMTTPDTGYQTLADIPTGSTLCVASPVSATGFW
                     AVAANELHDGLDFRVGGGDFELIVNDHFVNPQNMVDNADCVAGIFIPEAATPYMVTDD
                     MVVMYEGQSPFQLYNTFSPVE"
BASE COUNT          605 a          985 c         1000 g          699 t
ORIGIN      
        1 cacgacgctg gatcaaccca ccgctggtgc ccgctccgca gaagaggagc ggctcattgc
       61 cctccagaaa cgcgataccc ggcgcaggtg gttttggcgg atcgcgggtt atgtggtctt
      121 cctcgccatg tgggcgctca cctcgcggat agtgggcgag aacctcatcc cgagcccgag
      181 ccggatcatc gagacctttc ctgagatagt cgagagcggc cggttccttt ccagctttgc
      241 cgcgacattg actcgaatcg ggatcggatt cgggttgtca tttctcatag gtatcgccct
      301 cgggatcttc acgcagagca aatggctcga cggcttcttc aaggacgcgg tgaccatcag
      361 cctcgtctcg cccgggttga tctgggcctt gaccggtttg gtgatctggg ggttcgaacc
      421 acagggttgg atcttcgcca tcgtgatgac caccttcgcc ctggtcacgg tgaacgtggc
      481 cgaagggatc cgcgcactcc ccaaggacct gatcgacatg tcgcgggcgt ttggggtcag
      541 cgggtggcgt cgtcagcgag agatcgtcct ccccttcctc gcccctttca tcttcaccgc
      601 tctccgtttc gggttctcgg tgggatggaa ggtgacggtt ctcaccgagg tcttctcctc
      661 caatgagggg atcggattcg agatgagggt cgcctcccag ttgtttcgga tggacgagct
      721 gttcacctgg gcgctctcct tcctggtctt cgccctcatc ctggagaagc tcgttctcca
      781 acgttacgag aacaagttct tccgctggag acaggaggtc accgtctgat ggcgaccatg
      841 gtctccgggg cagcaacccg cgaatcgcga ttcgccagag tgttgaagtc gccggccacg
      901 gcaagactcc tgttcccgtt cgtcgtgctc gcggtttggt acctgatcta ctacaccatc
      961 gactcccgca tcttccccac cccgcaggag gtgctcgagt tcatgtggga ggaagtgacg
     1021 ctcacgagcg gagtgcgcta cagcagtgtc gccgagaacc tctacgttca gttcggcgta
     1081 tcgatcgttc gactgacgat cggtttcttc gtcgcactga tcttggggac tgtgatcggt
     1141 ctggccatgg gcatctccaa gtcggtcgat gccttcttcc acgactgggt catgggggtg
     1201 ctggccatgc ccgctctggt ctgggccttg ttcctcggtc tcgccctcgg attcaccaat
     1261 gtcggcccga tcatcgccgt cgtcctggcc gggatcccgt tcgtgatcat caacgtgcgg
     1321 gaaggcgtgc gcaacactcc caaggagctg ttcgacatgg ctcgggcctt cgacgtcccg
     1381 cagaacaaga tcacccgcca cgtgcttctt ccatccctgt tgccgttctt cttcgccgcc
     1441 atccggtatg catatgccat cggatggaag ggcctgatca tcaccgaggt gttcgcctcc
     1501 gatcggggca tgggctggac catcaagttc tggtatgacg ccaaccgggc caaaggtgtc
     1561 atcgggtacg ccctcttctt catcatcttc gccatgatcc tcgagaaatt ggtcttcgat
     1621 cgactgtcga cgaaggcgtt caggtggcgt ccacagatca acgcactcgc aatggcggaa
     1681 ggagacttcg gcggagtcac ccaggaaggt ttccttctcc aggagagcca ggtccaggaa
     1741 ggtgtgccta tcgagagcat ggaggtcgag tcgagagatg gctgagatca aggtcgagaa
     1801 cttgcacaag gagttcgggg tcggagagga tcgcgtcgtc gctctcgaga atgtgaacat
     1861 gcaggtgcag ggtcatgtgt tcgtgtccat cgtcgggcct tccggttgcg ggaagtcgac
     1921 tctgctcaac atcctgagtg ggatcgaaac cccgagttcg gggaccgtga ccatcaccca
     1981 ggacaatcgc gacgccgagc ccggatatgt ctttcaggct gcccgacttc tcccctggcg
     2041 gagcgtgatt gacaatctgc tgttcgtcca gaaggacaag agtgacgcaa ccagggagcg
     2101 ttgccagaag tacctggaga tggtccaact cggcgacaag gcggacaaat acccgggtga
     2161 gctgtccggt ggcatgcagc agagggttgg gatcgctcgg gccttctcca tcgagcccga
     2221 cgtcctgttc atggatgagc cgttcagtca tttggacgcc atcactgccc gctctctgcg
     2281 acgtgagctc cacgacatgt gggagcagac ccgaaagaca gtcctctttg tcactcacga
     2341 tgtgggcgag gcggtggagc tctccaaccg tatcctcgtc tttgccaagg ggggccggct
     2401 ggaggacgac cttcaggtcg agctcccctt tccccgggac ccggccgacg accaggtcgc
     2461 ggtggcaaaa gcccatgtgc tgcgcaaatt cgaagagctc gacttggtgg ttagctgaac
     2521 ctgacgatcg tccgcaccgt acccggggtc gatgccgacc tgttgatgaa aatggaggag
     2581 aagatgggca aacgatcccg atggctggtg tcgctggttg cagttctggc gatggtgatc
     2641 accgcctgcg gcgacgacac cgacggtgaa ggcactgacg gcactgccga tggcgaggca
     2701 cgaaccgtca tccgcttcgc cttcgccccc gatcccgtct gggactacat gaacgacaac
     2761 ggatacctcg tcgattggga gcaggagaac aacatccgga tcgtgaccag cacgacctgg
     2821 gacgagttca ccttcttcgc cggcggtcat ggcgacgtgg tgtcgatggg aaccctcgag
     2881 atccctgtcc tggagcagga gacggaaatc cagacagtga cgtttggcaa gtacaacgca
     2941 cttcgtgact cgatgatgac cacacccgac accggatatc agactctggc ggacattcca
     3001 acgggctcca ccctgtgcgt ggccagcccg gtgtctgcca ccggcttctg ggccgtggcg
     3061 gccaacgagt tgcacgatgg cctcgacttc cgggtcggtg gcggcgactt cgagttgatc
     3121 gtcaacgacc atttcgtcaa cccgcagaac atggtggaca acgctgactg cgtcgccggg
     3181 atcttcatcc ccgaggccgc gaccccctac atggtgaccg atgacatggt ggtgatgtac
     3241 gagggccagt cgccgttcca gctgtacaac acgttctcac ctgtcgaga
//
LOCUS       DATITW010000368         1761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_659200, whole genome shotgun sequence.
ACCESSION   DATITW010000368 DATITW010000000
VERSION     DATITW010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659200"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VLS86_05225"
     CDS             <1..688
                     /locus_tag="VLS86_05225"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HSO49926.1"
                     /translation="TVNLAVNALLALDLGFRPVIGVLPSGTGCDLLRTFGLPQDRAGA
                     ARHLTTTDTYDIDVATLEGEWGLRYFVNVAQTGVGAAAVESAARIGRWFGSARYPLAF
                     AARLPRFPRANVTVTTERLTYESEALAVIFANAQFFAGGWNVAPKATLVDGVLDIQII
                     DARKTQAPALVPKLIKGTHLGDPSVRRLSAAEFTVESEPVWPVECDGDLIGNTTVRGR
                     VVRAAISLKI"
     gene            741..1223
                     /locus_tag="VLS86_05230"
     CDS             741..1223
                     /locus_tag="VLS86_05230"
                     /inference="COORDINATES: protein motif:HMM:NF025204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HSO49927.1"
                     /translation="MAKEKVEELAEEVDLEVEEIDDDDVPDLDEDAITELDDDIEDVE
                     IDPDFEEVFEEDEPAVVGFEPVADDEDEEEDVDEETTEALEELESEELELLDEEAGEA
                     LLVDEVEELRAIRREELTMNVDAQLQRSDEFVCQSCFMVKRTSQLANRKKMLCADCAY
                     "
     gene            1213..>1761
                     /locus_tag="VLS86_05235"
     CDS             1213..>1761
                     /locus_tag="VLS86_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HSO49928.1"
                     /translation="MPTDRVTVVIPTYNERENLSHIAAAVTHQGYRLLVVDDSSPDGT
                     GELADRLAMSEPRMSVLHRRRKQGLGPAYAAGFDRALSQGAEIVIEMDADFSHDPADL
                     PRLVAAIDDGADLAIGSRYVAGGATPDWPTMRRLISKGGNLYARIMLGIEIRDSTAGF
                     RAFRADALRSLPYQQARASGYAF"
BASE COUNT          343 a          525 c          592 g          301 t
ORIGIN      
        1 aacggtgaat ctggcggtga atgccctcct cgcgttggat ctcggatttc gcccggtgat
       61 cggagttcta ccctcgggca ccggctgtga cctgctgcgc acctttggcc tgccacagga
      121 ccgggccggg gcggcgcgtc acctgacgac caccgacacc tacgacatcg acgtcgcgac
      181 cctcgagggg gagtggggcc tgcgctactt cgtgaatgtg gcccagaccg gggtgggagc
      241 ggccgcggtg gagagcgcag cccggatcgg gcgatggttc ggctcagccc gctatccgct
      301 ggccttcgcc gcccggcttc cccggtttcc aagagccaat gtcacagtca cgaccgaacg
      361 cctaacgtat gagtccgagg ccctggccgt catcttcgcc aatgcccagt tcttcgccgg
      421 gggttggaac gtggcgccga aggcgacact ggtcgacggc gtgctggaca tccagataat
      481 cgatgccagg aagacgcagg cgccggccct ggtccccaaa ctgatcaagg gcacgcatct
      541 cggagatccg tcggtgcgcc gactctccgc cgccgagttc acggtcgaat cggagccggt
      601 gtggccggtc gaatgcgacg gggacctgat cgggaatacc acagttcggg gtcgtgttgt
      661 gcgggccgcg atcagtctca agatctgaga cgcttcgtct tatactcccg cgcccctgag
      721 ggtcagaact gagcgaaatc atggccaaag aaaaagtcga agagctggct gaagaggtcg
      781 acctcgaggt cgaggagatc gacgacgacg atgtccccga tctggacgag gacgcgatca
      841 cggagctgga tgacgacatc gaagacgtcg aaatcgatcc cgatttcgaa gaggtgttcg
      901 aagaagacga gccggcagta gtcggattcg agccggtggc ggacgacgag gacgaagagg
      961 aagacgtcga cgaggagacc accgaggcct tggaggagct ggagagcgag gagctcgaat
     1021 tgctcgacga ggaggccggc gaggcgcttc tcgtcgatga ggtggaggag ctgcgtgcga
     1081 ttcgccgcga ggagctcacg atgaatgtcg atgcccagct tcagcgatcg gacgaattcg
     1141 tgtgccagtc ttgcttcatg gtgaagagga cgagccagct cgccaatcgc aagaagatgc
     1201 tgtgcgcaga ctgtgcctac tgaccgggta acggtcgtaa tccccaccta caacgagagg
     1261 gagaacctct cccacatcgc cgcagcagtg actcatcagg gctacaggct tctggtggtg
     1321 gacgactcct cacctgatgg caccggtgag ctggccgacc ggctggcaat gtccgagccg
     1381 aggatgtccg tcctccaccg gcggcggaag caaggtctcg ggcctgccta tgccgcgggg
     1441 ttcgatcgcg ccctgagcca gggtgccgag atcgtcatcg agatggacgc cgacttctcc
     1501 cacgacccgg cagacctccc aaggctggtg gcggctatcg acgacggggc cgacctggcg
     1561 atcggatccc ggtacgtggc cggaggggcg accccggatt ggccgacgat gagacgtctc
     1621 atctcgaaag gcgggaatct gtacgccaga atcatgctcg gaatcgagat tcgcgattcc
     1681 accgccggat tcagggcgtt tcgggccgat gcgctgcgct ctcttcccta tcagcaggca
     1741 agagcctccg ggtacgcgtt t
//
LOCUS       DATITW010000369         3158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_739541, whole genome shotgun sequence.
ACCESSION   DATITW010000369 DATITW010000000
VERSION     DATITW010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739541"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VLS86_05240"
     CDS             <1..292
                     /locus_tag="VLS86_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HSO49929.1"
                     /translation="KISALEIEDVILRHESVAEAAVVGIEDPEWGQRVVAVVVATQPV
                     TADELKQWVRNTLAPHKVPKEFHFTESLPRNALGKTMKPDVTKFVVEQTRPT"
     gene            303..689
                     /locus_tag="VLS86_05245"
     CDS             303..689
                     /locus_tag="VLS86_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSO49930.1"
                     /translation="MPQLDAVGVIVSDMGRAIDFYRRVGLDFPAEARDEVHAEARGPG
                     GLRVMLDTEASVASFSDWQAPTGGSPRTALAFLCDDPAQVDRVFGELVAVGGQGHLPP
                     FDAPWGQRYASVLDPDGNALDLFAPL"
     gene            complement(706..1626)
                     /locus_tag="VLS86_05250"
     CDS             complement(706..1626)
                     /locus_tag="VLS86_05250"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HSO49931.1"
                     /translation="MGRSPILRRWTRLALLVAVSLTTACAPESAPTTTTTATATTTTA
                     PSPTTAPGPDRPVVEVHTEEYLPGLAATVFSPVPPGAGVLVMVPGGGWQTADPAGFAD
                     LARYLAETGLVVVTVEVRAAVDDVVYPVPVEDVMCAVGFSAEWAEAQGQPSGPLVVLG
                     HSTGAHLAALAALAGPDELPECSPPALLPDALVGLAGTYDVAHLPEVAVSLFGVPPEE
                     DPDLWDRGNPVMRAGARPEVPVLLIHGAEDELVPRSFTDEFASALDSAGHRTTVEIIP
                     AADHHQIYRPESTGSLIGDWAHSLAGLPSE"
     gene            complement(1638..2561)
                     /locus_tag="VLS86_05255"
     CDS             complement(1638..2561)
                     /locus_tag="VLS86_05255"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSO49932.1"
                     /translation="MSSKPETTPEDTDLGSPSRIGFVGLGRMGSRIAANLTTHHEVRV
                     WNRTPQVAEQWVAANGGAVADSLSGLAASSEYLITMLADGEALVDVFESAGGLTEGID
                     SHTVAIDMGTSGPESFERVRSMVEARGGTMVDAPVSGATAAAESASLLVMVGAAPEVY
                     ERVAPVLGLVGNPVHVGPTGAAAVLKLAVNSVLYGLNQAVGEAVALAERAGVAPETTL
                     DVLARGAAGAPMLTYRREQYLHPDQAPVTFTVDLALKDLTLTMEQARRSGAPIGQLER
                     TRELMEDLVERGFGSEDMGYVVEASRRHQDR"
     gene            2681..>3158
                     /locus_tag="VLS86_05260"
     CDS             2681..>3158
                     /locus_tag="VLS86_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49933.1"
                     /translation="MAAFIVSLLAVTACRDGQPSSDDSAAAATTSVTSATTSSADPEP
                     TTSTTAVSTTLPPTTPSTTDATTTTIALPPFPPERESLTHGGDTWVVVLGASEEIDDP
                     VLDDAEARAADAGYTTGATDCDFGAASALGLGEDTHYYTVSVYLSTEADAEAALDAF"
BASE COUNT          527 a         1082 c         1063 g          486 t
ORIGIN      
        1 gaagatctcg gcgctcgaga tcgaagacgt gatcttgcgc cacgaatcgg tcgccgaagc
       61 cgcggtcgtc ggcatcgagg acccggagtg gggccagcgg gtggtggccg tggtcgtggc
      121 cacacaaccg gtgacagccg acgagctcaa gcagtgggtg cgcaacaccc tggccccgca
      181 caaggtccca aaggagttcc acttcaccga gtcactgcct cgcaacgccc tgggcaagac
      241 catgaagccg gacgtgacga agttcgtcgt cgaacagacc cgcccgacat agactcgggg
      301 ccatgcccca actcgatgct gtcggtgtca tcgtctcgga catgggccga gccatcgact
      361 tctatcggcg ggtcggcctg gacttccccg cagaagccag ggacgaggtc cacgccgagg
      421 ccagaggccc gggcgggctg cgggtgatgc tggacaccga ggccagtgtc gcgtctttct
      481 ccgattggca agctcccacc gggggcagcc ctcgcacggc actggcgttt ctctgcgacg
      541 acccagctca ggtggatcgg gtcttcgggg aattggttgc ggttggaggg cagggccatc
      601 tgccgccatt cgatgccccg tgggggcagc gctacgcctc ggtcctcgac ccggacggca
      661 acgccctgga cctcttcgcc ccgttgtagc cgagctcgac cgcggtcact ccgagggcag
      721 accggccaga ctgtgcgccc aatcaccgat cagcgatccg gttgactcag gccggtagat
      781 ctggtgatgg tcggcggcgg ggatgatctc gacagtggtg cgatggccgg ccgagtcgag
      841 agcgctcgcg aactcgtccg tgaacgacct ggggaccagt tcgtcttccg cgccgtgaat
      901 cagcaacacc gggacctcgg gtcgagcacc ggcacgcatc accgggttgc ctctgtccca
      961 caagtctggg tcctcttcgg gggggacgcc gaacagggag accgccacct cggggagatg
     1021 cgccacgtcg taggtgccgg ccagcccgac cagggcgtcg gggagcagcg ctggaggaga
     1081 gcactccggc agctcgtccg ggccggcgag agcggccaac gcggccaggt gggcccccgt
     1141 ggaatgaccg aggaccacca gcggaccgga aggctggcct tgtgcctccg cccactccgc
     1201 ggagaatccc acggcgcaca tgacatcctc gaccggcacc gggtagacga cgtcgtcgac
     1261 tgctgccctc acttcgacgg tgacgaccac caatcccgtt tccgccaggt accgggcgag
     1321 gtcggcgaaa ccagcggggt cggcggtctg ccacccgccg ccgggaacca tgaccaggac
     1381 gccggcacct ggtggaacgg gagagaagac tgtcgccgcg agaccgggga ggtactcctc
     1441 ggtgtgcacc tcgaccacgg gccggtcagg gccgggcgcg gtggtagggc tgggcgcggt
     1501 ggtggttgtg gcggtggcag tggtggtggt agtgggggcg ctctccggag cgcatgcagt
     1561 ggtcagggag accgccacca gcagtgcgag acgcgtccac cggcggagga tcggagatcg
     1621 acccaacagc cccccgttta ccggtcctgg tgccgtcggc tggcctcgac cacgtatccc
     1681 atgtcctcgg agccgaagcc acgctcaacc agatcctcca tcagctcccg ggtccgctcc
     1741 agctgaccga tcggggcgcc gctgcgtcgc gcctgctcca tggtcagcgt caggtccttc
     1801 agtgcgaggt cgaccgtgaa tgtcaccggc gcctgatccg ggtggagata ctgctcgcga
     1861 cggtaggtga gcatgggtgc tcccgccgcc ccgcgcgcca ggacatcgag tgtggtctcc
     1921 ggtgccaccc ctgcccgctc ggctagggcc actgcctccc ctaccgcctg gttcaacccg
     1981 tagaggacgg agttgaccgc cagcttgaga acggctgcgg cgcctgtggg cccgacgtgg
     2041 accgggttgc caaccagtcc gagaacaggc gccacccgct cgtagacctc gggagcggcg
     2101 ccgaccatga cgagcaggga tgccgactcc gcggcagcag ttgctcccga gaccggggca
     2161 tccaccatcg tcccgcccct cgcctcgacc atcgatcgga cccgctcgaa gctctccggg
     2221 cctgaggtgc ccatgtcgat ggccacggtg tgcgagtcga tgccctcggt gaggcctccc
     2281 gccgactcga agacatcgac cagagcttcg ccgtcggcaa gcatcgtgat caggtattcg
     2341 ctggacgccg ccaggcctga gaggctgtcc gccaccgcgc caccgtttgc cgccacccat
     2401 tgctccgcaa cctggggggt gcggttccag accctcacct cgtggtgcgt ggtgagattg
     2461 gctgcgatcc gggagcccat gcgtccgaga ccgacgaacc cgattcggga tggggagccg
     2521 aggtctgtgt cttcaggtgt cgtttcaggc ttggatgaca tggcagcgac gagtatgcac
     2581 cacgacgacg acggatgctt cggccacaat cacgcggcaa tgccctgaat ctgacaaggt
     2641 tgacgagtct catggagaaa cggctttgaa ccgggcgctg ttggcggcct tcatcgtgtc
     2701 cctgctcgcg gtgaccgctt gccgcgatgg ccagccgagc agcgacgact cggcggccgc
     2761 ggcgacgacc tcggtcacga gtgccaccac gagctccgcc gatcccgagc cgaccacatc
     2821 caccactgcc gtgtccacca ccctccctcc gaccacgccc agcaccaccg atgccaccac
     2881 gacaacgatc gcccttcccc cgttccctcc cgagcgtgag agcctgaccc atggtggcga
     2941 cacctgggtg gtggtcctgg gtgcatcgga ggagatcgac gacccggtgc tggacgacgc
     3001 cgaggctcga gctgcggacg cgggatatac cacgggcgcc accgactgcg acttcggggc
     3061 ggccagcgcc ctgggtctgg gcgaagacac gcactactac accgtcagcg tctatctgtc
     3121 gaccgaggcg gacgccgagg cagcactcga cgccttcg
//
LOCUS       DATITW010000370         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_178588, whole genome shotgun sequence.
ACCESSION   DATITW010000370 DATITW010000000
VERSION     DATITW010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178588"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VLS86_05265"
     CDS             <1..1386
                     /locus_tag="VLS86_05265"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSO49934.1"
                     /translation="NQVPLGVTRTYVKSVDIGVWPLARRAFQVTRCNQCDDAPCVAAC
                     PTSAMYRRADGIVDFDKRICIGCKACIAACPYDAIFINPEDHSAEKCNFCTHRLDIGL
                     EPACVVVCPTEAILVGDVNDPSSKVARIVNREPVTVRRPEKETHPKLFYKGAHQATLD
                     PIAARRPSGDLYMWSEQGEGVRSGHPGGWSNSSAAAVLSYDIPHKAPWDWRVSLYTWT
                     KSIAAGVYLAPVLLMLLGVEAFAAFGILPSVLGLPFLAVTGALLVWDLEHPKRFYYIF
                     TRPQWRSWLVRGGAIIVGYGLVLVLSLFATESIRALLVIAGLPLSLMAAAYTAYLFAQ
                     AKGRDLWQSPLLPPHMAVQAVLAGAAALLVVTGDEWPVATVLTWASVIHLLFVLGETT
                     LPHGTAHAHLAVWELTRGRYASFFWTGVALVLIGSLAPWIGVWAAPAALIGLLAHEHA
                     YVQAGQAVPLA"
     gene            1383..>1758
                     /locus_tag="VLS86_05270"
     CDS             1383..>1758
                     /locus_tag="VLS86_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HSO49935.1"
                     /translation="MRYPGPSGIHLDSFPPKDQWDDWTEFESRAWPERVERHSMLVPT
                     TCFNCESACGLLAYVDRQTLEIRKLEGNPEHPGSRGKNCAKGPATINQITDPDRILHP
                     LKRSGRRGEGKWTEVSWNEAMDD"
BASE COUNT          283 a          601 c          562 g          312 t
ORIGIN      
        1 aaccaggtgc cgctcggggt aacacggacc tacgtgaagt cggtcgacat cggcgtctgg
       61 ccgctcgcac ggcgggcctt ccaggtgacc cgctgcaacc agtgcgacga cgctccctgc
      121 gtggccgcct gccccacctc cgctatgtac cgccgggcag acgggatcgt cgacttcgac
      181 aagcggatct gcatcggctg caaggcctgc attgccgcct gcccgtacga cgccatcttc
      241 atcaaccccg aggaccactc agccgagaag tgcaacttct gcacccaccg actcgacatc
      301 gggctggagc cagcctgcgt ggtggtgtgc cccaccgagg cgatcctggt cggagatgtc
      361 aacgacccct cgtccaaggt ggcgagaatc gtcaaccggg agccggtgac ggtccgccga
      421 ccggagaagg agacccatcc caagctcttc tacaagggcg cccaccaggc gaccctcgac
      481 cccatcgccg cgaggcgacc gtccggtgac ctatatatgt ggtccgagca aggcgagggg
      541 gtgcggtcgg gccatcctgg cgggtggtcg aactcctcgg cagcagccgt gctttcctac
      601 gacatccccc acaaggcgcc gtgggattgg cgggtgagtc tgtacacgtg gaccaagtcc
      661 atcgccgccg gggtctacct ggcgccggtg ctgttgatgt tgttgggggt cgaggcgttc
      721 gccgcattcg gcatccttcc gtcggtgctt ggactgccct tcctggctgt gaccggggcc
      781 ttgttggtct gggaccttga gcatccgaag cgcttctact acatcttcac ccgcccccag
      841 tggcgaagct ggctggtacg gggcggggcg atcattgtcg gttacgggtt ggtcttggtg
      901 ctgtcgctgt tcgcgaccga atcgatccga gcgctcctgg tgattgccgg actaccgctg
      961 tccctgatgg ccgcggccta cacggcctat ctgtttgccc aggcgaaggg ccgcgacctc
     1021 tggcaaagcc cgctacttcc gccccacatg gcggtccagg cggtgctggc cggagccgcg
     1081 gccctgctgg tggtcacagg tgatgaatgg ccggtggcga cggtgctgac gtgggcctcg
     1141 gtgatccacc tgctgttcgt gctcggggag accaccctcc cccacgggac cgcccatgcc
     1201 catctggcgg tgtgggagct gacccgtgga cgatatgcga gcttcttctg gaccggcgtg
     1261 gccctggttc tgatcggatc attggcaccc tggataggcg tctgggccgc cccggcggca
     1321 ctcatcggcc tcctcgccca cgagcacgcg tatgtgcaag ccggccaggc ggtgcccctg
     1381 gcatgaggta tccgggcccc agcggcatcc acctcgactc tttccctccc aaagatcagt
     1441 gggacgattg gaccgagttc gaatcccggg cctggccgga gcgagtcgag cgccactcga
     1501 tgctggtccc caccacctgc ttcaactgtg agtccgcctg cggtctcctc gcctatgtcg
     1561 accgccagac cctcgaaatc cgcaagctcg aaggcaaccc cgagcacccg ggctcgcgag
     1621 gcaagaactg tgccaaggga cccgccacca tcaaccagat caccgacccc gaccgcatcc
     1681 ttcaccccct caaacgttcg ggccggcgcg gcgagggcaa gtggacggag gtgagctgga
     1741 acgaggcgat ggatgaca
//
LOCUS       DATITW010000371         1708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_219025, whole genome shotgun sequence.
ACCESSION   DATITW010000371 DATITW010000000
VERSION     DATITW010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219025"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /gene="metK"
                     /locus_tag="VLS86_05275"
     CDS             <1..715
                     /gene="metK"
                     /locus_tag="VLS86_05275"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HSO49936.1"
                     /translation="ERLSEVRRTGKLDYLRPDGKTQVMVRYEGTRPIPEEKVLISAHH
                     APDKDQDEMAEEVFDTVVAAVVPEEMLTSELEFIFNPSGRFVIGGPVADTGLTGRKII
                     VDTYGGYARHGGGAFSGKDATKVDRSAAYAARWAAKNLVAAGVADKIEIQLSYAIGRA
                     EPFSIHDETFGTETVSPDTIERVVNEVFDFRPASVIAQLQLTKPVFAPTAAYGHFGRA
                     GFSWEQLNQVEAIRAAAGL"
     gene            805..>1708
                     /locus_tag="VLS86_05280"
     CDS             805..>1708
                     /locus_tag="VLS86_05280"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49937.1"
                     /translation="MVQLGSIVRVPLGGRKTRGYVVEVAVDRGGTLRDLGAVSGAAPV
                     FDAGLLKSLDWAATHYVAPVAILLDRAAPPNLPKSIGSVPGTRVDERQVEHPLTSVAR
                     AASEGKRRPVTAIATGGDSTDWLGALAPVLNAGKSVLVIAATATEVDVLAEAARSGFG
                     DRVVTVAGDAPNSVTRGWEVVQGPGHLLIGTPRVAAWLVARLALVVVIEEGRRAMKDR
                     QTPTIHVRDLMTTRSRVEGFALVFIGPTPSLEVIAAGGEVTRATPRAWPLVEVVDRGE
                     DPPGSGYLSERTISALRSAASAGRR"
BASE COUNT          278 a          557 c          575 g          298 t
ORIGIN      
        1 tgagcggctc tccgaggtga gaaggaccgg caagctcgac tatctgcggc ccgacggcaa
       61 gacgcaggtg atggtccgct acgagggaac ccgtccgatt ccagaggaaa aggtgctgat
      121 ctcagcccat cacgccccgg acaaggacca ggacgagatg gctgaagagg tcttcgacac
      181 ggtggtggcc gccgtcgtcc ccgaagagat gctgacctcc gagctggagt tcatcttcaa
      241 cccctccggc cggttcgtca tcgggggccc ggtagccgac accggcctca ccgggcggaa
      301 gatcatcgtc gacacctatg gcggttatgc acgacacggg ggtggcgcct tctcggggaa
      361 ggacgcgacc aaggtcgatc gttccgccgc ctacgcggct cgctgggcag ccaagaacct
      421 ggtcgcagcg ggagttgccg acaagatcga aatccaactc tcctacgcga tcgggcgtgc
      481 cgagccgttc tccatccatg acgagacatt cggcaccgag acggtctcac cggacacgat
      541 cgagcgggtg gtcaacgaag tgttcgattt ccggcccgcg tcggtcatcg cccaactcca
      601 actcacgaag ccggttttcg cacccacggc cgcctatggc cactttggca gggccggctt
      661 ctcctgggag cagctcaacc aggtggaggc aatcagagcg gcggccggtc tctgagtcga
      721 ccggcggctc gggttgtccc cagcatcccc acttttgccg tagacaaggg tttctggtac
      781 tcgatccccg atcatctcca agatctggtc cagctcggtt cgatcgtgcg ggttcctctc
      841 gggggccgca agacccgcgg ctatgtcgtc gaggtggctg tggaccgcgg cgggaccctc
      901 cgggacctgg gggcggtctc cggtgcggct ccggtgttcg acgccggcct gctcaagagt
      961 ctcgactggg cggctaccca ctatgtggct ccggtggcaa tcctcctcga tcgtgccgcc
     1021 ccgcccaacc tgcccaagtc gatcggcagc gtgcccggca cgcgggtcga tgaacgacag
     1081 gtcgagcacc cgctgacgtc ggtggcgcgg gcggcgagtg agggaaaacg acgcccggtg
     1141 accgccatcg ccaccggtgg agactccacg gattggctag gggcgctggc gccggttctc
     1201 aacgccggaa agtctgtact cgtcatcgcg gcgaccgcaa ccgaggtgga tgtcttggcc
     1261 gaggcagccc ggtccggttt cggggatcgt gtggttacgg tggccggcga tgctccgaac
     1321 tcggtgaccc ggggctggga agtggtccag gggccagggc acctcctgat tgggaccccc
     1381 agagtcgccg cctggctcgt ggctcgcctg gcgctggtcg ttgtcatcga agagggccgc
     1441 cgggcaatga aggatcgtca gacaccgacg atccacgtcc gcgacctgat gaccaccagg
     1501 tcgcgggtcg aggggttcgc tctcgtgttc atcggcccca ccccgtccct cgaggtcatt
     1561 gcagcgggag gtgaggtgac ccgagccact ccgcgtgcct ggcccctggt tgaggtcgtg
     1621 gaccgcggtg aagacccacc tggctcggga tacctctctg agcggactat ctccgctttg
     1681 cgctctgcgg catcggctgg gcgccgct
//
LOCUS       DATITW010000372         1549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_659343, whole genome shotgun sequence.
ACCESSION   DATITW010000372 DATITW010000000
VERSION     DATITW010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659343"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VLS86_05285"
     CDS             <1..432
                     /locus_tag="VLS86_05285"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49938.1"
                     /translation="TAMIAGFGEIVDPKPRLEGFAVAVVVPEHRLRTADVYRRWDKFE
                     GPVGEVVTPGLLPPPLREGIPIRNDLTPAAVDLEPALADFMADLRSAWGTVVAMTGSG
                     SACFGFFPDLDEAHDAARSVAAVCRGAVGAALRPRGVERVE"
     gene            complement(440..>1549)
                     /locus_tag="VLS86_05290"
     CDS             complement(440..>1549)
                     /locus_tag="VLS86_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSO49939.1"
                     /translation="CRGERMRIEAREIHPITMTLRTPMPMSNGVIESTGNVVVKLTTD
                     EGVIGWGEGVEAPALTNQSQSDIVADLEALRPLVLGSDPMRRTELWVRLMSGPGTGST
                     AIGAIDIAVHDLVGRALGIPVHQLLGGAVRDHIPALTLVGSGDGPSDADKLAEKHAQG
                     FRWFKIKLGMAAPDVELGTLAKAVELVGDQGVVCGDVNEGWDEREASRFLSQLDGSQI
                     RFIEQPVGRNHRDALLRLAESSPVQLCADESAGSLAAVIGFAGTAVGGVSLKLIKHGG
                     ITGVMRGAAICAGGGLEINLAGKVIESSISAAANLHCAAAMDRVGFGCSPANQGIVLD
                     VSQNPVGLDAGVFTVPDGPGLGVEVDEGMLARLAG"
BASE COUNT          234 a          555 c          502 g          258 t
ORIGIN      
        1 acggccatga tcgccggatt tggggagatc gtggacccca aacctcgtct cgaagggttc
       61 gcggtggcgg tggtggttcc cgagcaccgt ctccggactg ccgacgtcta ccgccgctgg
      121 gacaagttcg agggcccggt aggcgaggtg gttacgccgg ggctgcttcc gccaccgctc
      181 cgggaaggga tcccgatccg caacgacctg acaccggcag cagtcgatct cgagcctgcc
      241 ctcgccgatt tcatggccga cctcaggtcg gcctggggca ccgtggtcgc catgaccggt
      301 tccggctcgg cctgcttcgg gttcttcccc gatctcgacg aggcgcacga cgcggcgcgt
      361 tcggtggccg cggtgtgtcg gggtgcggtt ggcgccgcgc tcaggccacg gggcgtggag
      421 agggtggagt gagacccgct cagccggcga gacgcgccag catcccctcg tcgacctcga
      481 cgccgagacc ggggccatcg ggcaccgtga agacaccggc gtcgagcccg actggattct
      541 ggctcacgtc gagcacgatc ccctgattgg ccgggctgca cccgaatccg acccgatcca
      601 ttgccgccgc gcagtgcagg ttggccgccg ccgagatgct cgactcgatc accttgcccg
      661 ccaggttgat ctccaggccg ccgccggcgc agatggcggc gccccgcatc actccggtga
      721 tcccaccgtg cttgatcagc ttgaggctga cccccccgac cgcggttccc gcgaatccga
      781 tcaccgccgc caggctgccc gcgctctcgt cggcgcacag ctggactggc gaggattccg
      841 ccaacctgag cagagcgtcc cgatggttgc gaccgaccgg ctgctcgatg aaccggatct
      901 ggctcccgtc gagctgactc aggaaccggc tcgcctcccg ctcgtcccat ccctcgttga
      961 cgtcaccgca gaccacgccc tgatcgccga ccagctccac cgccttggcc agagtcccca
     1021 gttcgacgtc gggcgcggcc atccccagct tgatcttgaa ccagcgaaag ccctgggcat
     1081 gcttctcggc gagcttgtcg gcatccgacg gaccgtcgcc gctgccgacg agggtcaggg
     1141 ccggaatgtg atcgcggacc gcgccaccga gaagctgatg gaccggaatc ccgagcgctc
     1201 gtccgacgag gtcgtggacc gcgatgtcga tggccccgat cgccgtcgag ccggtgcccg
     1261 gaccgctcat gagccgaacc cacagctcgg tgcgtcgcat ggggtccgag ccgaggacga
     1321 gcgggcgaag cgcctcgagg tcggcgacga tgtcggactg actttgattg gtcaaggccg
     1381 gagcctcgac accttcaccc cacccgatca cgccttcgtc ggtggtcagc ttcaccacca
     1441 cgttccccgt cgactcgatg acaccgttgg acatcggcat cggggttcgc agggtcatgg
     1501 tgatcggatg gatctccctg gcttcgatgc gcatccgctc tcctctaca
//
LOCUS       DATITW010000373         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_178741, whole genome shotgun sequence.
ACCESSION   DATITW010000373 DATITW010000000
VERSION     DATITW010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178741"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VLS86_05295"
     CDS             <1..849
                     /locus_tag="VLS86_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HSO49940.1"
                     /translation="KIGGFEPAIGSMGKQQYEDVVQRARHHIGEGDVYQIVPSVQFTG
                     ETDLDPFTVYRGLRLLNPSPYMYFCDLGETKVIGSSPEALVRLRGTVAELRPIAGTRP
                     RHNDEQRDRIAEEELIGDAKEAAEHVMLVDLARNDLGRVAVPGSIKVDPYRAIERYSH
                     VIHLVSGVSGIMETGGDAFDLFAAAFPAGTVVGAPKIRALELLRELEPVPRGIYAGTV
                     GYFGHGGTMDQAIAIRTIVMSEGRYTYQAGAGIVADSVPSTEWEEVLSKSGAMRGALE
                     IAAGGL"
     gene            846..1526
                     /locus_tag="VLS86_05300"
     CDS             846..1526
                     /locus_tag="VLS86_05300"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HSO49941.1"
                     /translation="MSRVLLVDNYDSFTYNLVQALRILGAEVLVRTNDDVSLDEARDL
                     EPSHLVVSPGPGRPEAAGESMKLISGFLDQVPILGVCLGHQAIATAMGGTVDHAQELR
                     HGKSSPVYHDSASIYAGLPNPFDAGRYHSLAVMEGDLPADLVVTSFTSDGVIMGIRHR
                     SLPVEGVQFHPESVLTPHGELLLRNFLDLGAVKPPSPASPVLPPAGGEENPPSPASPV
                     LPPAGGEI"
BASE COUNT          293 a          534 c          551 g          303 t
ORIGIN      
        1 aagatcggtg ggttcgagcc tgcaatcggc tcgatgggga agcagcagta cgaggacgtg
       61 gtgcaacgtg cccgtcatca catcggtgag ggcgacgtgt atcagatcgt cccctcggtc
      121 cagttcacgg gggagaccga cctcgatccg ttcacggtct atcgagggct tcgactcctc
      181 aatccctctc catacatgta cttctgtgac ctgggcgaaa ccaaggtgat cgggtcctcg
      241 ccggaggcgc tggtcaggct gcgcgggacc gtggcagagc tgcgacccat cgccgggacc
      301 aggcctcgtc acaacgacga gcaacgcgac cggatcgcag aggaagagct gatcggcgat
      361 gccaaggagg cggccgagca tgtgatgctt gtcgacctgg ctcgcaatga cctgggcagg
      421 gtcgcggtgc ccggctcgat caaggtcgat ccgtatcgtg ccatcgagag atacagccac
      481 gtcatccatt tggtcagcgg ggtgagcggg atcatggaga cgggtggcga tgccttcgac
      541 ctgttcgccg ctgccttccc ggccggcacc gtggtaggcg cccccaagat ccgagccctc
      601 gagctgttgc gtgagctcga gcctgttccc cggggcatct acgcggggac cgtcggctat
      661 ttcgggcatg gtgggacgat ggaccaggcg atcgccattc gcaccatcgt catgagcgag
      721 ggccgctaca catatcaggc cggtgcgggc atcgtcgccg acagcgtgcc ctcgactgag
      781 tgggaagaag tcctctccaa gtcgggtgcg atgcgtggcg ccctcgagat agcggcaggt
      841 gggctgtgag ccgggtgctc ctcgtcgaca actacgactc gttcacctac aacttggtgc
      901 aggcattgag gattctcggc gcagaggtgt tggtccgaac caacgacgac gtgagtctgg
      961 acgaagcccg ggacctcgag ccctcccacc tcgtcgtctc accaggtccg gggcggcccg
     1021 aggcagccgg ggagtcgatg aagctgatct cgggattcct cgatcaagtg cccatcctcg
     1081 gcgtttgcct tggtcatcag gcgatcgcca ccgccatggg cggcaccgtg gaccacgcgc
     1141 aggagctgag gcatgggaag tcgtcgccgg tgtatcacga ctccgcttcg atctatgccg
     1201 gtctccccaa cccgttcgac gccgggcgct atcactcgtt ggccgtgatg gagggcgatc
     1261 tcccggcaga tctggtggtg acctcgttta cctccgatgg cgtgatcatg ggaatacgtc
     1321 atcggtcgtt gccagtggaa ggtgtgcagt tccaccccga gagcgtgctc acaccgcacg
     1381 gtgagctgct cctgcgaaac ttcctcgatc tgggagcggt gaaaccccct tccccggctt
     1441 cgccggttct cccccctgcc gggggagaag agaatccccc ttccccggct tcgccggtac
     1501 tcccccctgc agggggagag atatgagcca ggtgctcgaa gacctcctcc gcggcaccga
     1561 cctcaccgag cgccaagccg agaccctcct ccatgacctg accggtggcg agatggaccc
     1621 ggcggtcgcc ggagccatcc tcgccgcact gagggcgaag ggcgagacat ccgaggaggt
     1681 c
//
LOCUS       DATITW010000374         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_219183, whole genome shotgun sequence.
ACCESSION   DATITW010000374 DATITW010000000
VERSION     DATITW010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219183"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VLS86_05305"
     CDS             <1..786
                     /locus_tag="VLS86_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSO49942.1"
                     /translation="ITALDVPQIQIDRWGTWNDPRRHSTVLLQADPSATLDLVEDASV
                     EEFAATWADANATMRAALDERLAQDESPTGPSVARALGDLEFDVRVAASSMPVRDVDA
                     HTAHQARTISNRGASGIDGLVSTALGVASAGRRTLALAGDLSLLHDIGGFVVDAPGDV
                     VFVVIDNGGGGLFDLLPQADHAPGFDRLFVTPHGLDLERVVAAYGIGSSTVRVVEELG
                     PSIATLIRAGGTHVVVVPVERETDLKQRRALDDAARLVCAGLS"
     gene            complement(779..1897)
                     /locus_tag="VLS86_05310"
     CDS             complement(779..1897)
                     /locus_tag="VLS86_05310"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HSO49943.1"
                     /translation="MTDRFDSDLNDALSRAPDSAWARRDLALLGWGQATCIDPGTGAD
                     RFDRALRELRATGAPLALASFTFDENAAGSVVVVPEVLLEIDRAGTSFLIGSAQDLPS
                     PLPSVGFPPGRVIEADPVGWVSLVEDALAAIRTREVEKVVLSRRLEIELEDEIPPHLV
                     LRNLVRGEPASHTFLVDHFVGSSPELLISLHQGIARSISLAGSADLEDGLGLRSFDSD
                     KMLREHALAADSVEDALAGFCLSLERSPSRVATYGDIRHLSTTFEGAAHPGTLITDLL
                     SSLHPTAAVAGTPTKSALELIRDLEKHDRGRYAGPVGWLEPSGDGEFAIALRCGTIAG
                     PRATLYTGAGLVEGSDPALEFEETQIKLRPMLGALGLS"
     gene            complement(1890..>2105)
                     /locus_tag="VLS86_05315"
     CDS             complement(1890..>2105)
                     /locus_tag="VLS86_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49944.1"
                     /translation="PVIESVAGPEVAIVDPAPAVARQVERVAGSSRESGRLLLATSGD
                     PARLEQLAYDLAGIETDQPVLACNWND"
BASE COUNT          328 a          705 c          747 g          325 t
ORIGIN      
        1 atcaccgccc tcgacgtgcc ccagatccag atcgaccgct ggggaacgtg gaacgacccc
       61 cgccgtcatt cgaccgtgct gctccaggct gacccctcgg ccactctcga tctcgtcgaa
      121 gatgcctcgg tggaggagtt cgctgcgacc tgggccgatg ccaacgccac aatgcgggca
      181 gctctcgacg agcgtctcgc ccaggatgag tcgccgaccg ggccgtcggt ggccagggca
      241 ctcggcgatt tggagttcga cgtgcgggtc gcggcctcgt cgatgccggt gagagacgtc
      301 gatgcccaca cggcgcacca ggcgaggacg atctccaacc ggggcgcctc cggcatcgac
      361 gggctggtct ccaccgcgct gggcgtggcc tccgccgggc gccggacact ggctctggcc
      421 ggggacctct cgctgctgca tgacataggc gggtttgtcg tcgacgcccc gggcgacgtc
      481 gtgttcgtgg tgatcgacaa tgggggaggg ggacttttcg acctgctgcc tcaggcagat
      541 cacgcacccg gcttcgaccg tctcttcgtc accccccatg gcctcgacct ggagcgtgtc
      601 gtcgccgcgt acgggatcgg gtcttcgacg gtgcgggtag tcgaggagct ggggccttcg
      661 atcgcgaccc tgatccgggc cggtggcacc cacgtggtgg tggtcccggt ggagcgggag
      721 accgacctca aacagcggcg ggcgctggac gatgcggcca ggctggtctg cgccggtctc
      781 agctgagccc cagcgccccc agcatcggtc gcagcttgat ctgagtctcc tcgaactcga
      841 gagccgggtc ggacccctcc accagacctg cccccgtgta cagcgtcgct cttggtccgg
      901 cgatcgtccc gcatcgcagc gcgatggcga actccccgtc gccggacggc tcgagccagc
      961 cgaccgggcc ggcgtagcgg ccacggtcgt gcttctccag gtcccggatc agctccaggg
     1021 cagacttggt cggcgtgccc gcgactgcag cggtggggtg gagcgaggag aggagatcgg
     1081 tgatcagggt gcccggatgg gctgcgccct cgaatgtggt cgacagatgc ctgatgtcgc
     1141 cgtaggtcgc gacccggctg ggtgagcgct cgagtgatag gcagaaaccg gccagggcgt
     1201 cttcgaccga gtcggcagcc agggcgtgct cccgcagcat cttgtcgctg tcgaaggacc
     1261 tgagcccgag gccatcctcg aggtcggccg atccggccaa cgagatcgat cgggcaatcc
     1321 cctggtgcag cgagatcagg agctcgggag acgagcccac gaagtgatcg acgaggaagg
     1381 tatgagaggc gggctcgccc cggaccaggt ttctcaggac gaggtggggc gggatctcgt
     1441 cctccagctc gatctcgagc cggcgcgaca gcaccacctt ctccacttca cgcgtgcgaa
     1501 tcgcggcgag ggcatcctcg accagggaca cccaacccac cgggtcggcc tcgatcaccc
     1561 ggcctggagg gaatccgacc gacggaagag gggagggcag gtcctgggcg ctaccgatca
     1621 ggaagctggt gccggcgcgg tcgatctcga gcagcacctc aggcaccacc acgacggagc
     1681 cggccgcgtt ctcgtcgaag gtgaagctgg ccagggccag cggcgccccg gtggctcgca
     1741 actcgcggag agctcggtcg aagcgatcgg cgccggttcc cgggtcgatg catgtcgcct
     1801 gcccccagcc gaggagcgcc aggtcgcgcc gggcccaggc cgagtcgggc gcccgggaca
     1861 gggcgtcgtt gagatcggag tcgaaacggt cagtcattcc agttgcaggc taggaccggc
     1921 tggtcggtct cgatgccggc caggtcgtat gcgagctgtt cgagccgggc cgggtcacca
     1981 gacgtcgcca ggagcagtcg tccggattca cgtgaggaac cggccacacg ttcgacctgt
     2041 ctcgccacag ccggagccgg gtcgacgatg gcgacctccg ggccggccac gctttcgatc
     2101 accgg
//
LOCUS       DATITW010000375         2744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_459431, whole genome shotgun sequence.
ACCESSION   DATITW010000375 DATITW010000000
VERSION     DATITW010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459431"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VLS86_05320"
     CDS             complement(<1..528)
                     /locus_tag="VLS86_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HSO49945.1"
                     /translation="MIERVIGEIATGQIPLRRSDVTAQAERLLAVASPRHASQWAEVV
                     TDRVLGLGPLQPHFLDPSVTDILVNGPDEIWVDRGGELERVEDGFADPGEIYAMVERV
                     IAPLGLRIDRSAPMVDARLADGSRLHAVVPPAAVDHPIVAIRRFTQAITSIAQLHESG
                     TATADQVEQLSQAVKA"
     gene            complement(525..1088)
                     /locus_tag="VLS86_05325"
     CDS             complement(525..1088)
                     /locus_tag="VLS86_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49946.1"
                     /translation="MVGRGSTSGRFGVLGVESADPVLSLVSALGLGQVAGTALVIDLC
                     RDLTLRSGRTLADISADGPSIGELSPGRSGVALLSAGSLGVSESPPVISALATTWPAV
                     VVRCPPGVWDGPTVPVRALLPGLLQPVDETPAVWQPLLPGSRPSGPGPLLPVLRGGLV
                     RRMLSGRAAGRARWVRAWAPVWQMPWA"
     gene            complement(1153..1503)
                     /locus_tag="VLS86_05330"
     CDS             complement(1153..1503)
                     /locus_tag="VLS86_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49947.1"
                     /translation="MITSQVLREIAADGPDRLVIGHSGELVEIDGLPEPAPIPALVVG
                     EVTEAVKQVEAGMVVRYLNRETLWAVTGFLLDREVVARLPDGVDSAAGLIQAVRDAGY
                     EWQAVVPLTTETQD"
     gene            1528..2184
                     /locus_tag="VLS86_05335"
     CDS             1528..2184
                     /locus_tag="VLS86_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HSO49948.1"
                     /translation="MTDRIMIVVAAGRSLRFGTDKLMTPVNGVPLIAHTVAAVIGQVE
                     RCILVCREDQIPALSALDLDVALIPGGPSRTASELAGLSAVGGGADLIGIHDGARPLV
                     PSGLTESLFATAAEHGGAIPLIEPSIPLVHKADLSVAAGTAFAQTPQVFRGGELLAAY
                     VQAARIEYVGHDTAEVAQRFSKLEIRGIPGDPVNIKVTFPSDLALVSAALGASRSESR
                     "
     gene            complement(2144..2641)
                     /locus_tag="VLS86_05340"
     CDS             complement(2144..2641)
                     /locus_tag="VLS86_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSO49949.1"
                     /translation="MIRDAELEDAPDLARVHVSSWQRAYVGLIDQAFLDAMSVESRIE
                     RWNLLLGQDRTRVLVVEAEGSVVGFCSLGLDDRQGWGEIYAIYVAPERWGAGLGRELL
                     AAGETALSAAGHERALLWVLEGNLRARMFYERQGWVSAKPVRLENIGGSPVTEIRYEK
                     HLGPR"
BASE COUNT          489 a          956 c          901 g          398 t
ORIGIN      
        1 cgctttcact gcctgtgaca gctgttcgac ctgatcggca gtggcggtcc cggactcgtg
       61 gagctgggca atcgaggtga tcgcctgggt gaagcgtctt atcgcaacga tcggatggtc
      121 gacggcggcc gggggaacca cggcgtggag ccgactcccg tcggccaatc gtgcgtcgac
      181 cattggggcc gaccggtcga tgcggagacc cagcggagcg atcactcgct cgaccatcgc
      241 gtatatctcc ccggggtcgg cgaagccgtc ctcgacacgc tccagctcac ctccccggtc
      301 cacccagatc tcatcggggc cgttgaccag gatgtcggtg acggacgggt cgagaaagtg
      361 cggctggagg ggaccgagcc cgagcacccg gtcggtcacg acctccgccc actgcgaagc
      421 atggcgtggc gaggccaccg ccagaagccg ctccgcctgg gcggtcacgt ccgacctgcg
      481 caaggggatt tggccggttg cgatctcgcc gatgacgcgc tcgatcacgc ccatggcatc
      541 tgccaaaccg gtgcccaggc tctgacccat ctcgcccggc cggcagcgcg accggacagc
      601 atccggcgca ccaggcctcc acgcagcaca ggcaggaggg gaccggggcc tgacggccgc
      661 gagccgggga gaagaggctg ccaaacggca ggggtctcgt cgaccggctg gaggaggccg
      721 ggaagcaggg cgcgcaccgg gacagtagga ccgtcccaca cgcctggggg acaacggacc
      781 acaaccgccg gccaggtcgt ggccagggcc gagatcaccg gcgggctctc ggagaccccc
      841 agcgagccgg cggagaggag ggcgacgccg gatcgccccg ggctcagttc gccaatagaa
      901 ggaccgtcgg cagagatatc ggcgagggtg cgcccggagc gcagcgtcag atcccgacag
      961 agatcgatca ccagtgcggt gccggccacc tgacccaagc ccagagcgct gaccagcgac
     1021 aacaccgggt cggcagactc gacaccgagg accccaaacc tccccgaggt gcttcccctc
     1081 cccaccatgc tctcgaagct atctaccggt gacgggcggt acaagacctt cgaggatgag
     1141 tcgttgctcc gatcaatcct gggtttcggt ggtcagaggt accactgcct gccactcgta
     1201 tccggcgtcg cgaaccgcct ggatcaaccc cgcggcggag tcgaccccat cggggagacg
     1261 tgccaccact tcccgatcca ggagaaaccc ggtcaccgcc cacagcgtct cgcgattgag
     1321 ataccgaacc accatccccg cctccacctg cttcaccgcc tcggtgactt caccgaccac
     1381 gagggcgggg atgggtgccg gctcaggtag gccgtcgatc tccaccagct caccggaatg
     1441 accgatgacc aaccggtccg ggccgtcggc ggcgatctca cggagaacct gggaggtgat
     1501 cacaaggcac aaaggtaccc tccgctcatg accgaccgga tcatgatcgt ggtcgcagcc
     1561 gggcgcagcc tccgctttgg caccgacaag ctgatgactc cggtgaacgg ggtgcccctg
     1621 atcgcccaca ccgtggccgc ggtcatcggc caggtggaaa ggtgcatcct ggtgtgccgc
     1681 gaggaccaga tcccggctct cagcgccctg gatctcgatg tcgcgctgat ccccggcggg
     1741 ccgtcgcgga cggcgagcga actggcggga ttgagcgcgg tcggcggcgg agccgacctg
     1801 atcgggatcc acgacggggc ccgccctctt gtcccttccg gtctgaccga gagcctgttc
     1861 gccaccgccg ccgagcatgg gggagcgatc ccgctcatcg agccgagcat tcccttggtc
     1921 cacaaggccg atctctccgt agccgccggg accgccttcg cccagacacc gcaggtgttt
     1981 cgtggtggag agctgctcgc cgcctacgtt caggcggcga ggatcgagta cgtgggccac
     2041 gacaccgccg aagtcgccca gagattcagc aagctcgaga tacgtgggat tccgggggac
     2101 ccggtaaaca tcaaggtgac attccccagc gatctcgccc tggtcagcgc ggccctaggt
     2161 gcttctcgta gcgaatctcg gtgacggggg agccgccgat gttctccagc cgaaccggct
     2221 ttgccgacac ccagccctgt cgctcgtaga acatcctggc ccgcaggttg ccttccagca
     2281 cccagagcaa ggcgcgctcg tgcccggctg ctgacagcgc cgtctccccg gcggcgagga
     2341 gctctcggcc cagccccgcc ccccagcgct cgggggcgac atagatggcg tagatctcgc
     2401 cccagccctg gcgatcgtcc aggcccagcg agcagaaccc gacgaccgag ccctcggcct
     2461 cgacgaccag gacgcgggtc ctgtcctggc cgagaaggag gttccatcgc tcgatgcgac
     2521 tctcgacact catcgcgtcg aggaaggcct gatcgatcag gccgacatag gccctctgcc
     2581 agctggagac gtggacccga gccagatcgg gcgcatcttc gagctcggcg tccctgatca
     2641 cggggtgacg ataagggctt gacaaatggt cgggatctcc cgaccatacg ggaatggccg
     2701 accatcgggc gccggactac gagctcgccg agacgctgtc gctg
//
LOCUS       DATITW010000376         1555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_539832, whole genome shotgun sequence.
ACCESSION   DATITW010000376 DATITW010000000
VERSION     DATITW010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539832"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="VLS86_05345"
     CDS             complement(<1..1473)
                     /locus_tag="VLS86_05345"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HSO49950.1"
                     /translation="MGRRARHQTVLILLVTGVLGIGTVHSVEGAVADYLGRPIAQVRI
                     EQENAEVQDRAILELIETRVWQPLEMTGVRETIGHLFSLGRYEDVQVRASSSGDGVVL
                     VYDLVPLHHVRALEFRGPIRIGESRLRSAITDRLGPTPPVGRASDAVRILEDVYRDEG
                     YRRPVITSSTRVEHQPDRTTLIFDVMPGAQTVVGRITVEGGRSTPAVVLDRIDVRMGD
                     PFRPAALITSLDRYRAELQSQGYYQARATYLQRFSNEESSVDLTVIVETGPLVSLRFE
                     GDPIPDRERAELVPIARQRSVDEDLLEDSKRRIEDWLKGQGYRDARADYRREQTGETL
                     EIVISVTRGPVHRLNTVEIVGSSMTAELQALVRLKDGAPFVDAVFSADVAGMVVFYRR
                     SGFANVKITPSVSPAEGTGAAGERQVTARITVAEGARASIGNLTFEGNSSIEDARLQV
                     VAGLAPGQPFYEPQTEMAADAIAVEYLNRGYANARVDRTLT"
BASE COUNT          288 a          511 c          470 g          286 t
ORIGIN      
        1 cgtcagcgtg cggtccacgc gagcgttggc atatccacga ttcaagtact cgacggcgat
       61 cgcatcggct gccatctccg tttgtggctc gtagaacggc tgcccgggag cgagccccgc
      121 aacgacctgc agtcgtgcat cctcgatgga cgaattccct tcgaacgtca ggttgccaat
      181 ggaggcccgg gcgccttccg cgacggtaat gcgggccgtc acctgacgct cgccggcggc
      241 gccagtgcct tcggccggcg acactgaggg ggtgatcttc acgttggcaa agcccgagcg
      301 tcgatagaac acgaccatcc cggccacatc cgccgaaaag accgcgtcga cgaagggcgc
      361 tccgtctttc agtcgcacga gcgcctgcag ctccgctgtc atcgagctgc cgacgatctc
      421 aacggtgttc agacgatgaa cgggcccccg agtcacgctg atgacaatct cgagggtctc
      481 gcccgtctgc tctcgacgat aatcggcgcg ggcatcgcga tagccctgcc ccttcagcca
      541 gtcttcgatc cgccgcttgg agtcttccag aaggtcctcg tccaccgatc gctgacgcgc
      601 aatcggcacg agttccgcgc gctcgcgatc cggaatcggg tctccctcga agcggaggct
      661 cacgagtgga ccggtttcca cgatcaccgt gagatccacg ctggactctt cgttggagaa
      721 tctctgaagg tacgtggctc tcgcctggta gtagccttgc gattggagct cggcgcgata
      781 gcgatcgagc gacgtgatca gcgcagccgg gcgaaaggga tctcccatcc gcacatcgat
      841 gcgatccagc acgaccgccg gagtcgaccg cccgccttcc acggtgatcc gtcccaccac
      901 ggtttgagcg ccgggcatga cgtcgaagat caacgtcgtc cgatcgggtt gatgttcgac
      961 tctggtgctc gaagtgatga ccgggcggcg atacccttca tcccggtaca cgtcctcgag
     1021 aatgcgtacc gcatccgacg cccggcccac gggcggtgtt ggcccgagcc gatcggtgat
     1081 cgccgatcgc aatcgggatt caccgatcct gatggggcca cggaactcaa gggcccgcac
     1141 atgatgcagc ggtacgaggt cgtagacgag gacgacgccg tcacctgacg agctcgcccg
     1201 cacttgcaca tcctcgtaac gtccaaggct gaagagatga ccgatcgttt cgcgcacccc
     1261 cgtcatctcg agcggctgcc aaacgcgcgt ttcgatcagc tcgaggatgg ctcggtcttg
     1321 gacctcggcg ttctcctgct cgattcgaac ctgtgcgatc gggcgtccga ggtagtcggc
     1381 aacggcacct tcgaccgagt gcactgtccc gattcctaga acaccggtca ccagcagaat
     1441 caggaccgtc tggtgccggg cgcgacggcc cacgctcatc agaacgcgtg cctcacacga
     1501 atgtcgaggg aataggtttc atcttcgttc cgggtaaaga cccaggagaa tcggt
//
LOCUS       DATITW010000377         1816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_659571, whole genome shotgun sequence.
ACCESSION   DATITW010000377 DATITW010000000
VERSION     DATITW010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659571"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="VLS86_05350"
     CDS             <1..1168
                     /locus_tag="VLS86_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSO49951.1"
                     /translation="ALDVSDAGRPVEVRVEQNLVLPDVDMPTHDTASNDPAIWVFSGG
                     TTGLPKAVVQSHRSFANSTELYGKRTLRLTPEDVTIAVPKLYFGYAMGSNLFFPVSVG
                     ASAVLFADHPSPEAMLTRIERHRATVLVTVPSAINQMLSHGDASDHDITSLRLATSAG
                     EALPETLHRQWAEKYGVELFDGLGTAEMWHIFISNQIGEVRPGTLGRVVPGYQIKVCD
                     EDGEEVPTGEVGRLWVRGDSLGLGYWENPSATSDAVRGEWFAGGDLVSIDEEGFVTHR
                     GRADDAIKVKGRWFRPQEVESTLLDHPAVTDCAVVAVSDESGLARPVAFVVAGGVTEQ
                     ELIDWALEHLEAYKHPRRVYFVDRLPQTHLGKVDRGALKKRANAPLEAGADGAQ"
     gene            1219..>1816
                     /locus_tag="VLS86_05355"
     CDS             1219..>1816
                     /locus_tag="VLS86_05355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339459.1"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HSO49952.1"
                     /translation="MEPTILHADLDAFYASVEQLLDPALRGRPVLVGGGVVLAASYEA
                     RAHGIRAPMLTRDARRLCPEAVVVDGHFERYLDFSAQVMAILEDATPVIEQISIDEAF
                     LDVSGSTHLLGPPSKIARQMRARVRGEVGLPLSVGIASTKFLAKVASARAKPDGLVVV
                     GLGAELDFLHPLPVEAMWGVGRVTSANLHRFGIYTIGDL"
BASE COUNT          278 a          598 c          620 g          320 t
ORIGIN      
        1 cgccctcgac gtcagcgacg cggggaggcc ggtcgaagtg cgagtcgagc agaatctcgt
       61 ccttcccgac gtcgacatgc cgactcatga cacggcgtcg aatgacccgg cgatttgggt
      121 cttctccggt ggcaccaccg gtcttcccaa ggcagtggtc cagagtcatc gctcttttgc
      181 caactcgacc gagctctacg gcaagcggac gctccgtctg acgcccgagg acgtgaccat
      241 cgccgtgccc aagctgtatt tcggctacgc catgggatcg aatctgttct ttccggtctc
      301 ggtgggcgcc tctgcggtgc tcttcgcgga tcacccgagc ccggaggcga tgctcacccg
      361 gatcgagcgg catcgggcga cggtgctggt gactgtcccc tcggcgatca atcagatgct
      421 ctcccacggc gacgccagtg atcacgacat cacctcgctc cggctcgcca cctcggcggg
      481 ggaggcgctt cccgagaccc tgcatcggca gtgggcggag aagtacggag tcgagctttt
      541 cgacgggctc gggaccgccg agatgtggca catcttcatc tccaaccaga tcggagaagt
      601 gcggccgggc accctggggc gtgtggtccc gggctatcag atcaaggtct gcgacgagga
      661 tggtgaagag gtccccaccg gcgaggtcgg ccggctctgg gtgcgcgggg actcccttgg
      721 tctcggctat tgggagaacc cgtcggcgac ctcggacgca gttcgggggg agtggttcgc
      781 cggcggggat ctggtttcca tcgacgaaga gggctttgtc acccatcgcg ggcgggccga
      841 cgacgcgatc aaggtgaagg gcaggtggtt ccggccccag gaggtggaat ccaccctgct
      901 cgaccacccc gcggtgactg actgcgccgt ggtggccgtg agcgacgaat ccgggctcgc
      961 ccggccggtc gccttcgttg tcgccggcgg tgtcaccgag caggagttga tcgactgggc
     1021 gctcgagcac ctcgaggcct acaagcaccc gcgtcgggtg tacttcgtcg accggctccc
     1081 ccagacccat ctcggcaagg tcgaccgcgg cgccctgaag aagcgggcca acgcacctct
     1141 cgaagccggc gccgacgggg cccagtaagg gggagtgagt tggccgtttc tgtcccaacc
     1201 tcgggtgaca ctcgtcagat ggaaccgacc atcctccatg ccgacctcga tgccttctac
     1261 gcctcggtcg agcagcttct ggaccccgcg ctgagaggca gaccggtcct ggtcggcggc
     1321 ggggtggtcc tggccgcctc ctacgaggcg agggcgcatg ggatacgggc gccgatgctg
     1381 accagggacg ccaggcgtct ctgcccggag gcggtggtgg tcgacggtca tttcgagcgc
     1441 tatctcgatt tctcggccca ggtgatggcc atcctcgagg atgcgacacc ggtgatcgag
     1501 cagatctcga tcgacgaggc cttcctcgac gtcagcgggt cgacccatct cctcggcccg
     1561 ccctcgaaga tcgctcgcca gatgcgggcc cgggtcaggg gtgaggtcgg tctcccgctc
     1621 tcggttggga tcgcttccac caagttcctg gccaaagtgg cctctgcccg ggccaagccc
     1681 gacggcctgg tcgtggtcgg gctcggagcc gagctcgact tcctccaccc gctgccggtg
     1741 gaggcgatgt ggggggtggg ccgcgtcacg tcggcgaacc tccaccggtt cggcatatac
     1801 accatcggcg acctgg
//
LOCUS       DATITW010000378         1944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_580051, whole genome shotgun sequence.
ACCESSION   DATITW010000378 DATITW010000000
VERSION     DATITW010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580051"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /gene="gndA"
                     /locus_tag="VLS86_05360"
     CDS             complement(<1..822)
                     /gene="gndA"
                     /locus_tag="VLS86_05360"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HSO49953.1"
                     /translation="MTDRADIGVIGLGVMGANLALNLADNGFKVVAFNRTGSVTDAFI
                     AGEAGEKGIVPAGSIEEMVEMLEPPRVVLVMVQAGNPVDQVIATLSDYLDDDDIVIDG
                     GNSLFSDTERRQRLLEARGYLYLGMGVSGGEEGARHGPSIMPGGSRAAWEQVEPMLKA
                     IAAKAPDGKPCCEWIGTGGAGHFVKMVHNGIEYGDMQVIAEAYDLMRRGMSMSPESMS
                     AVFARWNEGRLRSYLVEITSAILATETEGLPIVDLILDAAGQKGTGKWTVVSSMDL"
     gene            889..1632
                     /locus_tag="VLS86_05365"
     CDS             889..1632
                     /locus_tag="VLS86_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007271730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSO49954.1"
                     /translation="MSTRVGVDVGGSGVKAGIVDVDSGVMISDRLRLDTPEPSTPDAV
                     AAAVKELVDQLDAPGPIGLGFPAVVRRGWVSTANNIDQSWIGVNALQVFEPMVGREVR
                     VINDADAAGLAEVRYGAAKDVEGKVLVLTFGTGIGSALLLDGKLVPNIEAGQLELNGA
                     RPAERIYSARARRAENLSWDQWGQRARDYITLANEVFTPDLIVIGGGISKYWDEFASY
                     VTFGEVRTTRAALANDAGIIGAAAVAFSE"
     gene            complement(1622..>1944)
                     /locus_tag="VLS86_05370"
     CDS             complement(1622..>1944)
                     /locus_tag="VLS86_05370"
                     /inference="COORDINATES: protein motif:HMM:NF039579.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HSO49955.1"
                     /translation="PELIVFNKADVMSPQALKRLASVYADSVAISALKGTGLEELLMK
                     LAELLERTMVTLTLEIPYSRGDVVAAVHRVGEVIEEKHDDNGMIFEVRIPEASRSQFV
                     EFTR"
BASE COUNT          339 a          628 c          633 g          344 t
ORIGIN      
        1 caggtccatc gaggagacca cggtccactt gccggtgccc ttctgaccgg ccgcatcgag
       61 gatcaggtcg acgatgggca ggccttcggt ttccgtggcg aggatcgcac tcgtgatctc
      121 cacgagatag gagcggagac gtccctcgtt ccatcgggcg aacaccgcag acatcgactc
      181 cgggctcatg ctcatccccc gccgcatcag gtcgtaggcc tcggcgatca cctgcatgtc
      241 cccgtactcg atcccgttgt ggaccatctt cacgaaatga ccggcgccac cggtgccgat
      301 ccactcacag cacggcttac cgtccggcgc cttggcggca atcgccttga gcatcggctc
      361 gacctgttcc caggcggcgc ggctgccgcc gggcatgatc gaagggccat gacgggcgcc
      421 ctcctcgcca ccggagaccc ccatcccgag atagagatag ccacgggctt cgagcagcct
      481 ctgacggcgt tcggtgtcgc tgaacagcga gttgccaccg tcgatgacga tgtcgtcgtc
      541 gtcgaggtag tcggacaggg tggcgatgac ctggtcgacg gggttcccgg cctgcaccat
      601 caccaggacg acccgggggg gctccaacat ctccaccatc tcttcgatgg aaccggccgg
      661 gactatcccc ttctccccgg cctcgccggc gatgaatgca tcggtcaccg acccggtccg
      721 gttgaaggcc accactttga atccgttgtc ggccaggttg agggcgagat tcgcccccat
      781 caccccgaga cctatcaccc cgatatctgc tctgtcagtc attgaaattc gagggtaacc
      841 gatcctgatt tagcacccgt agggcctgag cgcctaggct cgcccgccgt gtcaacgagg
      901 gtcggagtgg atgtaggtgg ttccggtgtc aaagccggga tagtcgatgt cgactcgggt
      961 gtgatgatca gcgatcgtct ccgactggac actccggagc cctcgacccc tgacgcggtg
     1021 gcggccgcgg tcaaggaatt ggtggatcag ctcgacgccc cgggcccgat cgggctcggg
     1081 ttccccgccg tggtgcgcag gggatgggtc tccacagcca acaacatcga tcagtcctgg
     1141 atcggggtga acgcgctgca ggtgttcgag ccgatggtgg gtcgggaggt gagggtgatc
     1201 aacgacgccg acgccgccgg gctcgccgag gtccgatacg gggcggcgaa agacgtggag
     1261 ggaaaagtcc tcgtcctcac cttcggcacc gggatcggga gcgcattgct cctcgacggg
     1321 aagctggtgc cgaacatcga agcggggcaa ctcgagctca acggtgctcg ccccgcggag
     1381 cggatctact cggccagggc gagacgtgcg gagaacctga gctgggacca atgggggcag
     1441 agggcgaggg actacatcac gctcgccaac gaggtcttca cccccgatct gatcgtgatc
     1501 ggcggtggca tctccaagta ctgggatgag ttcgcgtcct acgtgacgtt tggcgaggtc
     1561 aggacgactc gcgccgcact ggccaacgac gccgggatca tcggggcggc ggcggtcgcc
     1621 ttcagcgagt gaactcgacg aactgagagc ggctggcctc ggggatcctc acctcgaaga
     1681 tcatcccgtt gtcgtcgtgc ttctcctcga tcacctcgcc gacgcggtgc accgcggcaa
     1741 ccacgtcgcc tcgcgagtac gggatctcga gggtgagggt gaccatggtg cgttccagga
     1801 gctcggcgag cttcatcagc agctcttcga gcccggtgcc cttcaacgcg gagatggcca
     1861 cgctgtcggc atagacgctc gccagacgct tcagggcctg cgggctcatc acgtcggcct
     1921 tgttgaacac gatcagctcc ggca
//
LOCUS       DATITW010000379         1811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_139188, whole genome shotgun sequence.
ACCESSION   DATITW010000379 DATITW010000000
VERSION     DATITW010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139188"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VLS86_05375"
     CDS             complement(<1..326)
                     /locus_tag="VLS86_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO49956.1"
                     /translation="MELGLGGRPQTHYLETDLGYLAYQVFGEGERDIMFVTGALSNVD
                     AIWDEPSAVRFFDRLGTMGRVIQYDMRGSGVSDPIPGRTMWMTIEENVDDLRSVLRAA
                     GSERAV"
     gene            complement(404..>1811)
                     /locus_tag="VLS86_05380"
     CDS             complement(404..>1811)
                     /locus_tag="VLS86_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSO49957.1"
                     /translation="IQIVAQGTFRDPELGFADSSGAGSGFIISPDGLAVTNNHVVAGA
                     ATLEVFIGGDESTSHNASIVGVSECNDLALIDINESEPLTYLDWYDGEISPGLTVYAA
                     GFPLGDPEYTLTGGIVSKASAGGDLTGTSSIDHTIEHDANIQPGNSGGPLVAEDGTVV
                     AVNYAGGAVATTTAQFYGIASDLAQPVVTRLMDGDFESLGINGWAVFDEEAALSGVWV
                     AGVAAGSPAAGAEILPGDIITAMNGLPVGTDGTFKDYCDVIRTAGDNAIAVDVLRYDT
                     SEVLRGEINGNQPLQLAFSFADEIEDDVSVDDSGSTVGYSEYQSIVDDTGKIVIEVPA
                     EWTDIDPTPAFDDAGAEIPYILAATDRQAWTDTFDVPGVLFVKLGPTDDIPATLLEYG
                     DLSASCTDLGLVDYADPVFTGQYQVWNDCGGTTTSVVVLAAVPADSSYTVLMLVQIVT
                     DADLDALDRIFQTFNVIG"
BASE COUNT          311 a          581 c          610 g          309 t
ORIGIN      
        1 atgaccgctc gctccgatcc ggcagcgcgg agcaccgacc gtaagtcgtc gacattttcc
       61 tcgatggtca tccacatcgt gcgtcccggg atcgggtcgg agactcccga gcctcgcatg
      121 tcgtactgga tgacccggcc catggtgccg agccgatcga agaagcggac cgcagacggc
      181 tcgtcccaga tggcgtccac gttgctcaac gccccggtca cgaacatgat gtcccgctcg
      241 ccctcgccga agacctgata ggccaggtat ccgaggtccg tctccagata atgagtttgc
      301 ggacggcctc caagccccag ctccatcgga gcaggctaca gcccatgttc ggggctcatg
      361 cccgtggcat ccgccggtgc cacagctcaa atcggcggcg atgtcacccg atgacgttga
      421 aggtctggaa gatccgatcc agtgcgtcga ggtcggcgtc ggtgacgatc tggaccagca
      481 tgaggacggt gtaagagctg tcggcgggaa cggcagccag gaccaccacc gaggtggtgg
      541 tgcctccaca gtcgttccag acctggtact gaccggtgaa gaccgggtcc gcgtagtcaa
      601 ccaggccgag atcggtgcag ctcgcggaca gatcgccgta ctcgagcagg gtggccggga
      661 tgtcgtcggt ggggccgagc ttgacgaaga ggacaccggg gacgtcgaag gtatcggtcc
      721 aggcctgccg gtcggtggcg gccaggatgt acgggatctc ggcaccggcg tcgtcgaatg
      781 cgggcgtggg atcgatgtcg gtccactcgg ccgggacctc gatgacgatc ttgcccgtgt
      841 cgtcgacgat cgactggtac tccgagtaac cgaccgtcga gcccgagtcg tccaccgaca
      901 cgtcgtcttc gatctcgtcg gcgaaggaga aggcgagctg gagcggttgg ttgccgttga
      961 tctcgccgcg aagcacctcc gaggtgtcgt agcggagaac gtccacggcg atcgcgttgt
     1021 cgcctgcagt ccggatgacg tcgcagtagt ccttgaacgt cccgtcggtg ccaacgggaa
     1081 gaccgttcat cgcggtgatg atgtccccgg ggaggatctc tgcaccggcg gccggcgaac
     1141 cggcggccac ccccgccacc caaaccccgg agagggcggc ttcctcgtcg aacaccgccc
     1201 agccgttgat gccgagactc tcgaagtctc cgtccatgag cctggtcacc accggttggg
     1261 ccagatccga ggcgatcccg tagaactggg ccgtggtggt ggccaccgcc ccacccgcgt
     1321 agttgaccgc gacgacagtc ccgtcctcgg ctaccagcgg cccacctgag ttgccgggct
     1381 ggatgttggc gtcgtgctcg atggtgtgat cgatcgaaga cgtgccggtg agatccccac
     1441 cagcgctcgc cttggacacg atgccaccgg tcagggtgta ctccgggtcg cccaggggga
     1501 acccggcggc gtacacagtc aggccgggcg agatctcgcc gtcgtaccag tcgagatagg
     1561 tgagcggctc cgactcgttg atgtcgatga gcgccagatc gttgcattcg gacacgccca
     1621 cgatcgacgc gttgtggctc gtggactcgt cgccgccgat gaacacctcg agggtcgcgg
     1681 ccccggccac cacgtggttg ttggtcacgg ccagcccgtc ggggctgatg atgaagccgc
     1741 tgccggcgcc ggagctgtcg gcgaacccga gctcgggatc gcggaaggtc ccctgagcca
     1801 cgatctggat g
//
LOCUS       DATITW010000380         1850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_459718, whole genome shotgun sequence.
ACCESSION   DATITW010000380 DATITW010000000
VERSION     DATITW010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459718"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1630)
                     /locus_tag="VLS86_05385"
     CDS             complement(113..1630)
                     /locus_tag="VLS86_05385"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005701.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HSO49958.1"
                     /translation="MPDITRLDAPLVVGAGVAGLTVALGLSRSYVVTAPELGSTWWAQ
                     GGIAVALSTDDSPRRPADDTLSVGGGLGVKRVVDLLTAGGPAAVEKLIALGAEFDRDD
                     EGELMLGREGGPQTRRVIHADGDATGAEVMRALHAAIEAEERVTMIQGRVVDLVMVGD
                     RVAGVVTAEGKDRVVLLAPAVVLATGGAGRLYARTTNPVGVTGDGIVMAGRAGARLAD
                     LEFVQFHPTALLAGKDPMPLLTEALRGDGAILVDAHGRRFMDQYHPMAELAPLDIVAR
                     AIFWQYDKGLGAYLDARSIIDFSDRFPTVNAHAHSVGLDPTRDLLPVSPAAHYYMGGI
                     DADINGRTSVPGLWAVGECSSSGVHGANRLASNSLLEGLVFGTRVSADVAANLVEPSG
                     EPVAPQEAFDLPLVAGPALEDLRQIMWDRVGLVRTGDGLWQARNHILDLDPVLRRTIA
                     GRVAAELSLMVVLAALRRSESRGGHYRADYPELDPMQEHRTLIDPTRAEMAAVPR"
     gene            complement(1643..>1850)
                     /gene="nadA"
                     /locus_tag="VLS86_05390"
     CDS             complement(1643..>1850)
                     /gene="nadA"
                     /locus_tag="VLS86_05390"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HSO49959.1"
                     /translation="SMADNVAWETPNTEYVRPCNLCPHMKRITLDNIRTSLATLTHAI
                     EIPPEVADRARVAVFRMLEVGRQD"
BASE COUNT          305 a          668 c          599 g          278 t
ORIGIN      
        1 atcgccttgc tctgcgccgt ctgcgggaca cacgtatccg aagtgatgtc gcccccggcg
       61 ccgagatcct cggccagggc acgacccaca acctccacga aatcggcccg gttcatcgcg
      121 gcaccgccgc catctcggcc cgggtcggat cgatcagggt ccggtgctcc tgcatcgggt
      181 cgagctcggg atagtcggcc cggtagtgcc cgccacgcga ctccgagcga cgcagggcgg
      241 cgagcacgac catcagcgac agttccgccg cgaccctgcc cgcgatggtg cgacggagca
      301 cagggtcgag atcgaggatg tggtttctcg cctgccacaa gccgtccccg gtgcggacca
      361 agccgacccg atcccacatg atctgccgca ggtcctccag agccggtcct gccaccaacg
      421 gaaggtcgaa ggcctcctgg ggagccaccg gctcgcctga cggctccacc agattcgcgg
      481 ccacgtcagc cgagaccctg gtcccgaaca ccagtccttc gagaagcgaa ttggaagcga
      541 gacggttggc cccgtgcacc ccgctcgagc tgcattcacc cacggcccac agcccgggaa
      601 ccgaggttcg cccgttgatg tcggcgtcga tgccccccat gtagtagtgg gcggcaggag
      661 agacaggaag caggtcccgg gtcgggtcga gccccaccga atgggcgtgg gcgttcaccg
      721 tgggaaaccg gtcggagaag tcgatgatcg atctcgcgtc gagataggcg ccgagaccct
      781 tgtcgtactg ccagaagatg gccctggcga cgatgtccag cggcgccagc tccgccatcg
      841 ggtggtactg gtccatgaat cgcctgccgt gggcatcgac caggatggcc ccgtcccccc
      901 gcaacgcctc ggtgagcagt ggcatcgggt ccttgccggc gagcagcgcg gtcggatgga
      961 actggacgaa ctcgagatca gcgagacggg cccccgccct ccccgccatc acgatcccgt
     1021 caccggtcac ccctaccggg ttggtggtgc gcgcatagag ccgcccggcg ccaccggtgg
     1081 cgagaacgac agcaggagcc aagaggacca cccggtcctt gccctccgcc gtgaccactc
     1141 cggccactct gtccccgacc atcaccaggt cgacgacgcg tccttggatc atcgtcaccc
     1201 tttcttcggc ctcgattgcg gcgtggagag cgcgcatgac ctcggcaccg gtggcatcgc
     1261 catcggcgtg gatcacacgc cgggtctgag gcccaccctc gcgccccagc atcaactccc
     1321 cctcgtcgtc gcggtcgaac tcggcgccga gtgcgatgag tttctccacc gcagccggcc
     1381 ctcccgcggt cagcagatcg accacccgct tcaccccgag gcccccgccc acgctcagcg
     1441 tgtcgtcggc aggcctcctc ggtgagtcat cggtggacag ggcgaccgcg atcccgcctt
     1501 gggcccacca ggtggagccc agctccggag cggtcaccac ataggagcga gacaacccga
     1561 gggccaccgt caacccggcg actccggcgc ccaccaccag cggggcatcc agtctcgtga
     1621 tgtccggcat gtgggccccg cctcagtcct gacgaccgac ttcgagcatc cggaacacgg
     1681 cgacccgggc gcggtcggcg acttcgggcg ggatctcgat ggcgtgggtc agggtggcca
     1741 gagaggtccg gatgttgtcg agggtgatcc gcttcatgtg cgggcagagg ttgcagggcc
     1801 gcacgtactc ggtgttcggg gtctcccagg ccacgttgtc cgccatggag
//
LOCUS       DATITW010000381         1701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_179163, whole genome shotgun sequence.
ACCESSION   DATITW010000381 DATITW010000000
VERSION     DATITW010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179163"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VLS86_05395"
     CDS             <1..266
                     /locus_tag="VLS86_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49960.1"
                     /translation="EQGENMTEDEKSGVDTALAALKLTLENDAAETTEIRSRVDELIK
                     ASQAMATRLYEQAAQQSQVGEEVAADDDENIVEAEIIDDDDEA"
     gene            294..848
                     /locus_tag="VLS86_05400"
     CDS             294..848
                     /locus_tag="VLS86_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HSO49961.1"
                     /translation="MSERKPEIVQAEGRTDASALGIELPEDQAQAIELLLSHLEESRD
                     EATSYLDDLKRVAADFDNYRKRTTREQAVVLDRAAERVVRELLPVLDSFDAALATEPS
                     TDVERQLLSGMINTREQLLKALEAEGLEVVPSIGEPFDPEIHEPAGAPSGSGDLAVSQ
                     ELRRGYRLKGRVLRPAMVMLEARE"
     gene            845..>1701
                     /locus_tag="VLS86_05405"
     CDS             845..>1701
                     /locus_tag="VLS86_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HSO49962.1"
                     /translation="MNRDWVDKDFYEILGVSPDASAEEIKKSYRKLAQTYHPDANPDD
                     TKAEDRFKEISEAYATLSSPDQRKEYDQVRRMVATGGFGGMGGPGGGFGGQQVRVEDL
                     SDLLGGLGGLGDLFGFGSGGRGPAKGPDLQADLTIGFEDGVKGVETAVTVRGADTCSH
                     CGGSGAEPGTQITTCPTCGGRGMVAQNQGFFSFSQPCPQCRGAGRLIETPCSVCRGTG
                     SETRTRQVKVRIPAGVQDGNTLRLRGKGGPGRNGGPNGDLLVRVRVAPSHTFGRKGND
                     VTLTVPITY"
BASE COUNT          323 a          536 c          589 g          253 t
ORIGIN      
        1 aggagcaggg cgagaacatg accgaagacg agaagtcggg agtggacacc gccctcgccg
       61 cactcaagct gacgctggag aatgacgcag ccgagaccac cgagatccga tccagggtcg
      121 acgagctgat caaggccagt caggcgatgg ccaccaggct ctacgagcag gctgcccagc
      181 aatctcaggt cggtgaggag gtcgccgcgg atgacgacga gaacatcgtc gaggccgaga
      241 tcatcgacga cgatgatgag gcgtgagagc cttgttccca ggacgtgcca acgatgagtg
      301 agcgaaagcc ggagatagtc caggcggagg ggcggaccga cgcctccgcc ctggggatcg
      361 agctgccgga ggatcaggcc caagccatcg agctcctcct ctcccatctc gaggagtccc
      421 gggacgaggc gacgtcgtat ctcgacgacc tgaagcgggt ggccgccgac ttcgacaact
      481 accgcaagcg caccacccgt gagcaggcgg tggtcctcga tcgggcggcg gagagggtgg
      541 ttcgggagtt gctcccggtt ctcgactcgt tcgacgccgc cctcgccacc gagccctcaa
      601 ccgatgtgga gcgacagctc ctgtccggga tgatcaatac ccgcgagcag ctcctgaagg
      661 ccctggaggc agaaggtctg gaggtggtcc cctcgatcgg tgagccgttc gatcccgaga
      721 tccacgagcc cgccggcgct cccagtggga gcggcgatct cgccgtgtcc caggagcttc
      781 gccgcggata ccggctcaag ggccgggtcc tcaggccggc gatggtgatg ctggaagccc
      841 gagagtgaac cgcgactggg tcgacaagga tttctatgag atcctcggcg tcagccccga
      901 cgccagcgcc gaggagatca agaagtccta tcgcaagctg gcacagacct atcaccccga
      961 cgccaacccc gacgacacga aggccgagga caggttcaag gagatctccg aggcatacgc
     1021 cacgctctcc agccccgatc agcgcaagga atacgaccag gtgaggcgaa tggtcgccac
     1081 cggtggattt ggcgggatgg gaggccccgg tggcggcttc ggcgggcagc aggtgcgtgt
     1141 cgaagatttg tccgatctgc tcggtggtct cggtggtctc ggcgatctgt tcggtttcgg
     1201 ctcgggcggg agagggccgg ccaagggccc ggatctccaa gccgacctga ccatcgggtt
     1261 cgaagacggg gtcaagggtg tggaaacggc cgtgaccgtc cgcggcgccg acacctgctc
     1321 gcactgcggt ggaagcggcg cggagccggg gacccagatc accacctgtc ccacttgtgg
     1381 gggacggggc atggtggccc agaaccaggg cttcttctcc ttctcccagc cctgccccca
     1441 atgccggggc gcgggccggc tgatcgagac tccttgcagc gtgtgtcgtg gcacgggctc
     1501 ggagaccagg acccgtcagg tcaaggtgcg gatcccggcc ggtgtccagg acgggaacac
     1561 ccttcgtctc cggggcaagg gcggccctgg ccgaaatggc gggcccaatg gcgatctcct
     1621 ggtcagagtg cgtgtcgccc cgagccacac cttcgggcgc aagggaaacg acgtcacgct
     1681 gacggttccc atcacctata g
//
LOCUS       DATITW010000382         1620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_139313, whole genome shotgun sequence.
ACCESSION   DATITW010000382 DATITW010000000
VERSION     DATITW010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139313"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VLS86_05410"
     CDS             complement(<1..287)
                     /locus_tag="VLS86_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49963.1"
                     /translation="MSIDIAGFVTDFKEHATEHGFHVHDERHFVETYSLRQSWEVDLH
                     PEAGCGGPIDLHVALDVDPKVILSLRERLDEMDSDFEEPENVYHLDLFFNWA"
     gene            complement(330..1331)
                     /locus_tag="VLS86_05415"
     CDS             complement(330..1331)
                     /locus_tag="VLS86_05415"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HSO49964.1"
                     /translation="MIDRLIQRFPRWARTPAEILAAAANEYGLDRASRMSAAIAYRTV
                     FAFAPLLIIGVAVLGAFLGSDLEAQQEIIGAIEEFAGPEVAQVVASVLDSALDSVDTA
                     AVIGGLLLLWTASSLFLELQHDLNDVFGVPYERVRGPLATVRSRGVGFLWAFGLGIIL
                     IAIWLLNALWRFLGGLLPPSMSNVHEVVGFLTPVVSLALLPVLFGLVFQTMTAVTLNW
                     RAVWVGGAFTAAVFVAASYGIGFYFEIFDSPSALGFAGSFVLIIFLAYFLSAVFLFGA
                     EVTKVYARRLEQGSEVAPTPAASPDPLVVVAQPGDSLPQAAFLAFLAGLLVGWRRRR"
     gene            1367..>1620
                     /gene="ligC"
                     /locus_tag="VLS86_05420"
     CDS             1367..>1620
                     /gene="ligC"
                     /locus_tag="VLS86_05420"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="catalyzes the ATP-dependent formation of a
                     phosphodiester at the site of a single-strand break in
                     duplex DNA; in mycobacteria LigC has weak intrinsic nick
                     joining activities and is not essential for growth;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HSO49965.1"
                     /translation="MSHPFPPPIETMEARTSEKWPKGEYLYEPKWDGFRSVSWSAPEP
                     RIDSRNQRPLLRYFPELEPALDQLPEGTVVDGEIVVVMNGV"
BASE COUNT          365 a          508 c          502 g          245 t
ORIGIN      
        1 gcccagttga agaagagatc gaggtgatag acgttctccg gctcttcgaa atcggaatcc
       61 atctcgtcga gacgctcgcg aagggacagg atgaccttcg gatcgacatc cagcgccacg
      121 tggagatcga tcggcccgcc acaacccgcc tcgggatgaa gatcgacttc ccatgattgg
      181 cgaagcgagt acgtctccac gaagtgccgc tcgtcgtgga cgtggaaacc gtgctcggtt
      241 gcgtgctctt tgaagtcggt gacgaaacct gcgatgtcga tggacattga tcgcgaggtt
      301 attggagtgc cggatcaaat gacagcgggt cagcggcggc ggcgccaacc gaccaacagc
      361 ccggcgagaa aggcgaggaa ggcggcctga ggcaggctgt ctcccggctg ggcgacaacc
      421 accagcgggt ctggggacgc cgctggggtg ggcgcaacct cactcccctg ctcgagtctc
      481 ctcgcataga ccttggtcac ctcggcaccg aagagaaaga ctgccgacag gaagtaggcg
      541 aggaagatga tcaacacgaa ggatccggcg aagccgagcg cggatgggct gtcgaaaatc
      601 tcgaagtaga acccgatccc gtatgaggcg gcgacgaaca ccgctgccgt gaatgcgccg
      661 ccaacccaga ccgcccgcca gttgagggtg accgcggtca tcgtctggaa caccaatccg
      721 aagaggacag ggagcaacgc cagcgatacc acgggggtca gaaacccgac gacctcgtgg
      781 acgttgctca tggaaggtgg caggagacct cccagaaacc gccagagcgc gttcagcagc
      841 cagatcgcga tcaggatgat cccgaggccg aaagcccaca agaacccgac tccacgagag
      901 cggacggttg ccaggggccc gcgcacccgc tcgtagggga cgccaaacac gtcgttcagg
      961 tcgtgctgca actcgagaaa cagactcgag gccgtccaca acagcaacaa cccaccaatc
     1021 accgccgccg tgtcgacact gtccagtgcc gaatcgagga cagaggcgac cacctgcgcg
     1081 acctcgggtc cggcgaactc ctcgatggct ccgatgatct cctgttgtgc ttcgagatcg
     1141 ctcccgagaa aagccccgag cacggcgaca ccgattatga gcaaaggggc gaatgcgaac
     1201 actgttcgat aggcaatggc cgctgacatc ctgctggcgc ggtccaggcc atactcgttt
     1261 gccgccgcgg ccaggatctc ggcaggcgtc ctcgcccatc tcggaaatcg ctgaatcagc
     1321 cggtcgatca cagccacgag ggtatcgccg catacactca ggccgagtga gtcacccctt
     1381 cccgccaccc atcgagacga tggaggccag gacatccgag aaatggccga agggggagta
     1441 cctctatgag ccgaaatggg acggctttcg atcggtgtcc tggtccgctc ctgagccgag
     1501 aatcgactcc cggaaccaga gacccttgct ccgctacttc cccgagctgg aacccgcgct
     1561 cgaccagctc ccggagggca ccgtcgtcga cggcgagatc gtcgtcgtga tgaacggtgt
//
LOCUS       DATITW010000383         1942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_99680, whole genome shotgun sequence.
ACCESSION   DATITW010000383 DATITW010000000
VERSION     DATITW010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99680"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VLS86_05425"
     CDS             complement(<1..200)
                     /locus_tag="VLS86_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HSO49966.1"
                     /translation="MTVDFDRNLSHQDLGLTDDQMIEIYRLMLTARRIDDRMFALQRQ
                     GRAPFVVGSSGHEAVQVASAYAL"
     gene            complement(212..1294)
                     /locus_tag="VLS86_05430"
     CDS             complement(212..1294)
                     /locus_tag="VLS86_05430"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002057686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HSO49967.1"
                     /translation="MVVFDEVAHGGHEQVLYGYDKVSGLRAIIAIHSTALGPALGGTR
                     FFPYQSEEDALEDVLRLSKGMTYKAAAAGLDLGGGKAVIIGDPRTDKSERMLRAYGRI
                     VDSLPGRYITAEDMGTTTGDMDVIRRETRWALGNSIAEGGSGDPSPVTARGLFAAARA
                     VAELIWGSADLSERKFAVQGAGKVGSALIQLLVEARAEVIISDSYEPAIAAAVETYGV
                     KSVEPEDIHTVDCDIFSPCARGAGLNQETIPALNCQAIVGSANNQLATDEDADAIAGR
                     GILYAPDFVVNAGGLINVYDELHGYSKMRAMQRVDGLFEATKTILEMSKREGITPNQA
                     AMQVAENRIRDIGDLRRFRRSGDDRN"
     gene            1405..>1942
                     /locus_tag="VLS86_05435"
     CDS             1405..>1942
                     /locus_tag="VLS86_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49968.1"
                     /translation="MTKTSYLRVYSPEGADSGDPVPGFVRAYGLLSETEGEGDWVADW
                     SGTRMICPRNLRLRVLESTVAFANAFRGLGSGLIPEEAARAADRELRQYHREHPSHRS
                     HVLTSAWHVPVRWFTAFQPEDKEVYEVDGSPRLRLRVDLPVAIRRVEHALGVLRGLEV
                     FQGPAEELAQLLEWIEGFP"
BASE COUNT          309 a          641 c          617 g          375 t
ORIGIN      
        1 agtgcgtacg ccgatgcgac ctgaaccgcc tcatgtcccg acgagccgac cacgaacggg
       61 gcccgaccct gacgttgcag ggcgaacatc cgatcgtcga ttcgtcgcgc ggtgagcatc
      121 aaccggtaga tctcgatcat ctgatcgtcg gtcaacccga ggtcctgatg gctcaggttc
      181 cggtcgaagt ccactgtcat ctcacggtcg ctcaattccg gtcgtcccca cttctgcgga
      241 atctccggag atcacctatg tcccggatcc ggttctcggc gacttgcatc gcggcctggt
      301 tgggggtgat cccttcacgc tttgacatct cgaggatcgt cttggttgct tcgaataatc
      361 cgtcgactcg ttgcatcgcc cgcatcttgg agtagccgtg caactcgtcg tagacgttga
      421 tcagaccccc ggcgttgacc acgaagtccg gggcatagag gatccctcgc ccggcgatgg
      481 catcagcgtc ctcatcggtg gcgagttggt tgttggcact gcccacgatg gcctggcagt
      541 tgagggcggg aatcgtctcc tggttgagac ccgctccccg ggcgcaaggc gagaagatgt
      601 cacagtcgac ggtgtggatg tcctcgggct cgacgctctt gaccccgtag gtctccaccg
      661 ctgcggcaat ggccggctcg tacgaatcag agatgatgac ctcggctcgc gcctccacca
      721 gcagttggat gagggccgat ccgaccttgc ccgcaccctg caccgcgaac ttccgctccg
      781 acaggtcggc gctcccccaa atcaactcgg cgacggcccg ggccgcggcg aacagaccgc
      841 gcgcagtgac cggtgaaggg tcccccgacc cgccctctgc gatcgagttg ccgagggccc
      901 aacgtgtctc ccgtctgatg acgtccatgt ccccggtggt ggtccccatg tcctcagccg
      961 tgatgtagcg acccggcaat gagtcgacga tcctcccgta ggcgcggagc atcctctcgc
     1021 tcttgtcggt cctgggatcg ccgatgatca cggctttgcc cccgccgagg tcgagacctg
     1081 cggccgccgc cttgtaggtc atgcccttgg agagacgcag cacgtcctcg agggcgtcct
     1141 cctcggattg gtacggaaaa aagcgagtcc cgccgagggc gggaccgaga gccgtggagt
     1201 gaatggcgat gatcgccctg aggccggata ccttgtcgta gccgtagagg acttgctcgt
     1261 gtcctccgtg ggctacctca tcgaatacga ccaaggtggg tgcactcctt gtccctgacg
     1321 gatgtcactc ccggccgata ctacccaccc cccgatccgg cgggccctgg tgatcttgcc
     1381 ctcgacctat gccaggatgg agaggtgacg aagacctcct atctccgggt gtactcaccc
     1441 gaaggggccg actccggcga cccggtgccg ggcttcgtcc gcgcctacgg tttgctctcc
     1501 gagacagagg gggagggtga ctgggtcgcc gactggagcg gcacacggat gatctgcccc
     1561 cgcaatctcc ggttgcgggt tctcgagtcg actgtcgcct tcgccaacgc gtttcgaggt
     1621 ctgggctcgg ggctgatccc ggaggaggcg gcaagggccg ctgaccgtga gctccgccag
     1681 taccaccgcg agcacccgtc tcatcggtcc cacgtgctca cctcggcatg gcatgtcccg
     1741 gtgcggtggt tcaccgcatt ccagcctgag gacaaggagg tctacgaggt cgacggttca
     1801 ccgcgtctgc ggctgagagt cgatctcccg gtggcgatca ggcgggtgga gcacgctctg
     1861 ggcgtcctgc gtgggctcga ggtgttccag ggccccgccg aggagttggc ccagctgctc
     1921 gaatggatcg agggcttccc cc
//
LOCUS       DATITW010000384         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_299657, whole genome shotgun sequence.
ACCESSION   DATITW010000384 DATITW010000000
VERSION     DATITW010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299657"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2296
                     /locus_tag="VLS86_05440"
     CDS             <1..2296
                     /locus_tag="VLS86_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSO49969.1"
                     /translation="AARLADGGLPAGAVLRDLGTHRLKDLTLPEHLYQLVLDDLDSEF
                     PAPITLDARPHNLPQQATEFLGRVSELDTIQLMLESPSTRLLTITGPGGAGKTRLGLQ
                     VAAEQLDRFRDGVFFVDLSAERDPDAAYEAIVRGLELPVSGGGDPLSILKSRLRDRRM
                     LLVLDNFEQVTTAAVGLSELLQHCPELKIVVTSREMLRVRAEHVYPVPPLSLPHPRDP
                     AAAIAESEAVQLFTERARAVRPGFSVTDENAPVIAEICLRLDGLPLAIELAAARLNVF
                     TPADLLDRLRERLDVLGAGGRDLPDRQRPLWGAISWSYELLDEAERRIFELMAVFSTT
                     DLRALEAVADSRSVIDSLASLVDKSLIRSEDSGNTQRFGMLLMIKEFAEERLADSPER
                     EQTVRRAHALYFSSFAERLQLRLRGLERESALDELALEIGNLRGAWRFWVDQGDLEQL
                     FNLIDGLWALHEAKGWYQAAIELASDILGVLATAEPSPELAAEELTLRTSLARALMAV
                     RGYGPEVEEMFKQALEICEVTGIAAQRFPVLRALATYYMGIADFEQGAELGRQLLELG
                     EEEDDEAIRIEGHYVLGAAVVFAGDLETGLPHLERAIELHDPKLHGSARFRLGPNTGV
                     AARVAFGLTLWQCGALEKAVSKVTEALSLARDIDHPYSVAYALYHNGFLALNRSRFEE
                     CAEWAGQLAAVADENDYVVWRTLATVLEGVSISALGRAEEGLAMTETAIELYQGLTTP
                     PVFWPLILGLRAWCTRWPANRSGL"
BASE COUNT          391 a          794 c          787 g          411 t
ORIGIN      
        1 cgcggcccgc ctcgccgatg gcgggctccc ggccggcgcc gtgttgcgcg acctgggcac
       61 ccaccgcctc aaggacctga ccctgcccga gcacctctac cagctggtgc tcgacgacct
      121 cgactctgag ttcccggcac cgatcacact cgacgccaga ccacacaacc tcccccagca
      181 ggccaccgag ttcctggggc gggtcagcga gctggacacg atccagttga tgctggagtc
      241 cccgagcacc aggctcttga ccatcaccgg ccccgggggt gcaggcaaga cccgtctcgg
      301 tctccaggtg gccgccgagc agctggaccg attccgtgac ggggtgttct tcgtcgacct
      361 ctccgcagaa cgagaccccg acgccgctta cgaggccatt gtgcgtggcc tggagctacc
      421 ggtctcgggt ggcggggacc cgctctcgat cctgaagtcg aggctccgcg accgtcggat
      481 gctcctcgtc ctcgacaatt tcgagcaggt gaccacagcc gccgtcgggc tctccgagct
      541 cctccagcac tgcccggagc tgaagatcgt cgtcaccagc cgggagatgc tccgggttcg
      601 tgccgagcac gtctacccgg ttcccccgct ttcgctgccc caccccagag atcccgctgc
      661 cgccatcgcc gaatcggagg cggtccagct cttcaccgag cgggctcgtg cggtgcgacc
      721 agggttttcg gtcacggacg agaacgcgcc ggtcatcgcc gagatctgtc tccggctcga
      781 cgggctgccc ttggcgattg aactggcggc agcacgtctc aacgtgttca ccccagccga
      841 tctcctcgat cggcttcggg agcgtctcga cgtgctcgga gccggagggc gggacctccc
      901 cgaccgacaa cgaccgctct ggggagcgat cagctggagc tatgagctgc tcgacgaggc
      961 cgagcgtcgc atcttcgagc tgatggcggt gttctccacg accgacctcc gtgcgctgga
     1021 agcagtggca gattcccgat cggtcatcga ttccctggcc tccctggtcg acaagagcct
     1081 gatccgaagc gaagacagcg gcaatacgca gcgattcggg atgctgctga tgatcaagga
     1141 gttcgccgag gagcgtctcg ccgactcccc ggagcgtgag cagacggtgc gccgcgccca
     1201 tgccctctat ttctcgtcgt tcgccgagag acttcagctg cgactgcgtg gcctggaacg
     1261 tgaatcggca ctcgacgagc ttgccctgga gatcggcaac ttgaggggag cttggcggtt
     1321 ctgggtcgac cagggtgacc tggagcagct gttcaatctc atcgacgggc tgtgggcact
     1381 gcacgaggcc aaaggctggt atcaggccgc gatcgagctc gcctccgaca ttctcggcgt
     1441 gctggcgact gccgagccgt cacccgagct ggcagccgag gagctgacac tgcgcaccag
     1501 cttggcccgc gcgctgatgg cggttcgggg ctacggcccc gaggtggagg agatgttcaa
     1561 gcaggccctg gagatttgcg aagtaaccgg catcgcggcg cagcgctttc cggtgctccg
     1621 cgccctggcc acctactaca tgggaatcgc tgacttcgaa caaggagctg agctgggccg
     1681 ccagcttctc gagctcggtg aggaggagga cgacgaggcg atccggatcg aggggcacta
     1741 tgtgcttgga gcggcggttg tgttcgccgg cgacctcgag acaggtctcc cccacctcga
     1801 gcgtgccatc gagctccatg atcccaagct tcacggctcg gcccgcttcc gtctcgggcc
     1861 caacaccggg gtggcggccc gggtcgcatt cgggttgacc ctgtggcagt gcggtgctct
     1921 ggagaaggca gtgagcaagg tcacggaggc gctcagcctg gcccgggaca tcgatcaccc
     1981 gtacagcgtc gcctatgcgc tgtaccacaa cggcttcctg gctttgaaca ggagtcggtt
     2041 cgaggagtgc gcggagtggg ccggtcaact ggccgcagtg gcggacgaga acgactacgt
     2101 ggtgtggcga acactggcaa ccgtcctcga gggagtctcg atcagcgccc tgggacgggc
     2161 cgaggaaggt ctggcgatga ccgagaccgc gatcgagctc taccagggtt tgaccacacc
     2221 accggtgttc tggccgctca tcctcggcct gcgggcatgg tgcacgcgct ggccggccaa
     2281 ccggagcggg ctctgaccct gatcgacgat gccatcgagc tcggctcgcc cgacgaggtg
     2341 gttgcgccgg aactcagaat gctcaagggt gacttcctcc gga
//
LOCUS       DATITW010000385         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_780360, whole genome shotgun sequence.
ACCESSION   DATITW010000385 DATITW010000000
VERSION     DATITW010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780360"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VLS86_05445"
     CDS             <1..400
                     /locus_tag="VLS86_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSO49970.1"
                     /translation="ISYLGQFLVFTYIWLPYMILPIHSSLERVPRSFLEASADLGAQP
                     RTTFRKVIWPLAIPGVAAGSIFTFSLTLGDFVIPTIIGDSSPFIGLAIYSYQGVAGNL
                     PLAAAFSFVPIAVMALYLTGARKLGAFEAI"
     gene            397..1182
                     /locus_tag="VLS86_05450"
     CDS             397..1182
                     /locus_tag="VLS86_05450"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017962179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49971.1"
                     /translation="MSRAGLGLKAASLGALLYLHIPILFVFLYAFTTEEASFRFPPPA
                     FTTQWFGVAAANPDIQSALSLSLRVATVSMVIALILGTLVAAAVWRTNFFGRQTLSLF
                     IILPIALPGIITGIALRSAINLGDVSFSFWTLVIGHATFCVAIAYNNVIARMRRTSPF
                     LMEASMDLGARGWQTFRHVLLPQLAPAMVTGGMLAFALSFDEIIVTTFTAGNQQTLPL
                     WIFSSLVRPRNRPVTNVVAVFVVLITFVPIVLAQLFTRRAEEN"
     gene            1198..>2075
                     /locus_tag="VLS86_05455"
     CDS             1198..>2075
                     /locus_tag="VLS86_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSO49972.1"
                     /translation="MVWDLWIDGKWTPGSGDKLDVENPATGDTVDVVTEASGEDVDAA
                     VNAANTAFFDGRWSRQTPGERARVIHKLADLLESRAEDFARVESEDTGKPYAQVSLGA
                     DLPFGVDNLRFFAAAARNIAGTASGDFASGYTSMLRREPVGPVGQIAPWNYPLLMAIW
                     KVGPALAAGCTTVLKPAPGTPRTALMLAELATEAGLPDGVLNVVTGGNETGQSLVEHP
                     LLRMVSLTGSTATGKKVMKTAADSLKRVHLELGGKAPFIVFDDADLESLAEAAMVGAC
                     FNTGQDCTAATRLYVE"
BASE COUNT          342 a          704 c          631 g          398 t
ORIGIN      
        1 gatctcatac ctgggccagt tcctcgtgtt cacctacatc tggctgccat acatgatcct
       61 ccccatccac tcctccctgg aacgggtgcc acggtcgttc ctcgaggcgt ccgccgacct
      121 cggagcccaa ccccgcacca cgttccgtaa ggtgatctgg cctctggcca tccccggagt
      181 ggctgccgga tcgatcttca ccttctccct gaccctcggt gacttcgtca tccccaccat
      241 catcggcgac tccagcccgt tcatcgggct tgccatctac tcctatcaag gggtggccgg
      301 aaacctcccg ttggcagcgg cgttctcctt cgtcccgatc gcggtgatgg ccctctacct
      361 gaccggggcc cgcaagctcg gagccttcga ggcgatatga gcagggcggg gctgggcttg
      421 aaggctgcct cgctgggggc gcttctctat ctccacatac ccatactctt cgtctttctc
      481 tacgcgttca ccaccgagga agcatccttt cgcttcccgc ccccggcctt caccactcag
      541 tggttcggcg tggccgccgc gaaccccgac atccagtcgg cgttgagcct ctccctgagg
      601 gtggccaccg tctcgatggt catcgcgctg atcctgggaa ctctggtggc ggcggccgtc
      661 tggcgaacga acttcttcgg caggcagacc ttgagcctgt tcatcatcct ccccatcgct
      721 ctgcccggca tcatcacggg catagccctg aggtcggcga tcaatctcgg ggacgtgtcc
      781 ttctccttct ggacactcgt gatcgggcac gccacgttct gcgtcgcaat cgcctacaac
      841 aacgtgatcg cacgaatgcg ccggacttcg cctttcctga tggaggcctc gatggacctc
      901 ggggcgaggg gatggcagac ctttcgccac gttctcctcc cccagctggc gccggcgatg
      961 gttaccggtg gcatgctggc cttcgccctc tccttcgacg agatcatcgt caccacgttc
     1021 actgcgggaa accagcagac tctcccgctg tggatcttct cctcgctggt gagaccccgc
     1081 aaccgcccgg tcaccaatgt ggtcgcggtc ttcgtggtgc taatcacatt cgtgccgatc
     1141 gtcctcgccc agctgttcac ccgccgggcc gaggagaact gagagacagg agaacacatg
     1201 gtctgggatt tgtggatcga tggcaagtgg acgccgggct cgggagacaa gctcgacgtg
     1261 gagaatccgg ccaccggaga caccgtcgac gtggtgaccg aggcatcagg cgaggatgtc
     1321 gacgcggcgg tgaacgccgc caacactgcg ttcttcgacg ggagatggtc ccgtcagacg
     1381 cccggagagc gggccagggt gatccacaaa ctcgcagacc tgctcgagtc gagggcggag
     1441 gattttgccc gtgtcgagtc cgaggacacc gggaagccgt acgcacaggt gtcgttgggg
     1501 gccgatctcc cgttcggggt ggacaacctg aggttcttcg cggctgcagc gcgcaacatc
     1561 gcgggtaccg cgtcgggcga cttcgcctcc ggatacacct cgatgctgag acgggagcca
     1621 gtggggccgg tcggtcagat cgcaccttgg aactatccgt tgctcatggc gatctggaag
     1681 gtggggccgg cgcttgccgc aggctgcacc acggtgctca agccggcccc ggggaccccg
     1741 aggacggcgc tcatgctggc cgagctcgcc accgaagcag gtctccccga cggtgtgctc
     1801 aacgtcgtga ccgggggcaa cgaaaccggc cagagcctgg tcgagcaccc tctgctgcgc
     1861 atggtgtccc tgaccggttc caccgccacc ggaaagaagg tgatgaagac cgcggctgac
     1921 agcctgaaac gggtccatct cgagctgggc ggcaaggcac cgttcatagt cttcgacgac
     1981 gccgacctcg agtccctggc tgaggcggca atggtcggcg cctgcttcaa caccgggcag
     2041 gactgcaccg ccgccacccg tctctatgtg gagaa
//
LOCUS       DATITW010000386         1994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_339747, whole genome shotgun sequence.
ACCESSION   DATITW010000386 DATITW010000000
VERSION     DATITW010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339747"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..961)
                     /locus_tag="VLS86_05460"
     CDS             complement(38..961)
                     /locus_tag="VLS86_05460"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007321488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HSO49973.1"
                     /translation="MSSEGQEMAIPLIEDASVAGVGDRSRVAAYFALTKPRIIELLLI
                     TTVPAMVLAAGGWPGTWLVMATLLGGSLSAGGANTLNNYVDRDIDQVMRRTRGRPLAR
                     HEVPPRNALVLGVLLGVAGFICLWLVANLVAAAISTAALLFYVFVYTIALKRTSAQNI
                     VIGGAAGAAPILVGWSAVTGSLALPAWVLFFVVFYWTPPHFWALAIRYRDDYTLAGVP
                     MLPVVAGVEATTRKMLLYTGLMVAVSLLLVPVAGMRWIYLVSALGLGAWFLWDTWRVY
                     RRPQDAMRLFTTSTVYLAALFAAVMLDVLVR"
     gene            complement(951..1124)
                     /locus_tag="VLS86_05465"
     CDS             complement(951..1124)
                     /locus_tag="VLS86_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49974.1"
                     /translation="MFTFAHYWGIDEVGVFVVPALLAIVALRWAERKAKKAAAAAGET
                     EPKPTAEKEIDVE"
     gene            complement(1124..>1994)
                     /locus_tag="VLS86_05470"
     CDS             complement(1124..>1994)
                     /locus_tag="VLS86_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49975.1"
                     /translation="EQVGVWPAGLLLLAFTWLELVSPSSGSPVVLGLAALVYTLFMVS
                     AMAYAGRETGLAVFDAFTPYNRVLSAISPIGRRDDNRLVWRGWLRALPVLPEWRGLPA
                     LVVIAIGSVTYDGASATTWFRSALADQSQTRLGQTMLLILTVLIIGLAYWASSLLAAR
                     MVGGPWTAPRVAARFAHTLVPIALAYAVAHYLTLVLFEGQQLIAAVSDPFALGWDVFG
                     TADRKIDFFLTASEPIWYLQVGVIVSGHVLGVVLAHDRALADFGPTAVRSQYAMLLLM
                     VALTTLGLLVLSG"
BASE COUNT          425 a          665 c          646 g          258 t
ORIGIN      
        1 agaagggcga gggcgacgac cgacagtgtc gctcttctca tcgaaccaac acgtcgagca
       61 tcaccgcggc gaacagcgcc gccaggtaca ccgttgatgt cgtgaacagg cgcattgcgt
      121 cctgggggcg tcgatacacc cgccaggtgt cccacaggaa ccaggcgccg agcccgagag
      181 cagaaaccag gtagatccat cgcatcccgg caaccgggac caggagcagg ctgaccgcga
      241 ccatgaggcc ggtgtagagc agcatcttgc gcgtggtcgc ctccaccccg gccacaaccg
      301 ggagcatggg gacaccagcc agcgtgtagt catcccgata ccggatcgcc agggcccaga
      361 aatgcggcgg cgtccagtag aagacgacga agaacaggac ccaagccgga agggccaatg
      421 acccggtcac cgccgaccac cccaccagga tcggcgcggc accggcggcg cccccgatca
      481 cgatgttctg ggcgctggtt cgcttcagcg cgatggtgta cacgaacaca tagaagagaa
      541 gggcagcggt cgagatggcg gccgctacga gattggcgac cagccagagg cagatgaagc
      601 cggcgacgcc gagcaggaca cccaagacca gggcatttcg aggcgggacc tcgtgtcggg
      661 cgagaggcct ccccctcgtc cgacgcatga cctggtcgat gtcccggtcc acgtagttgt
      721 tcagggtgtt ggccccaccc gcggaaaggg agcccccgag cagggtcgcc atcaccaacc
      781 aggtgccggg ccatccgccg gcggcgagga ccatcgccgg caccgtcgtg atcaacaaca
      841 gctcgatgat tcggggcttg gtcagggcga aataggcggc gacccgcgag cggtcaccga
      901 ctccggccac cgaagcgtcc tcgatcagcg ggatggccat ctcctggccc tcactcgaca
      961 tcgatctcct tctcagcggt tggcttcggc tccgtctccc ctgccgcggc ggcggccttc
     1021 ttcgccttcc gctccgccca gcgaagggcg actatggcca gcaatgcggg caccacgaag
     1081 accccgactt cgtcaatgcc ccagtagtgc gcaaatgtga acatcaccca gagaggacca
     1141 atagcccaag tgtggtcagc gcaaccataa ggagaagcat cgcgtactgg gagcggaccg
     1201 cggtcggtcc gaaatcggcg agcgcccgat cgtgggcgag gacgacaccg aggacatgac
     1261 cggagactat gacgcccacc tggaggtacc agatcggctc ggaggcggtg aggaagaaat
     1321 cgatcttgcg gtcggcggtg ccgaacacat cccagcccaa ggcgaaggga tcggacaccg
     1381 ctgcgatcag ctgctggccc tcgaacagaa ccagggtcag atagtgggca acggcatagg
     1441 ccagcgcgat cgggaccagg gtatgggcga accgtgccgc cactctcgga gccgtccacg
     1501 gtcctccaac catgcgggca gcgagcaatg aggaggccca atacgccagc ccgatgatca
     1561 acacggtcag gatcaacagc atcgtctgcc ccaaccgcgt ctggctctgg tcggccagag
     1621 ccgatcggaa ccaggtggtc gccgaggcgc cgtcgtaggt gacactcccg atcgcgatca
     1681 cgaccagggc ggggagtccc cgccattcgg gaagcaccgg aagcgccctg agccatcccc
     1741 gccacacgag ccggttgtca tcgcgtcgcc cgatgggtga gatcgccgag agaacccggt
     1801 tgtagggagt gaaggcgtcg aacaccgcca atccggtctc ccgcccggcg taggccatgg
     1861 cgctcaccat gaacaacgtg tagaccaggg cggccagacc gagcacgacg ggcgaccccg
     1921 agctcgggct caccaattcg agccaggtga aggccagaag caggagaccc gcgggccaga
     1981 cgcccacctg ctcc
//
LOCUS       DATITW010000387         1517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_59732, whole genome shotgun sequence.
ACCESSION   DATITW010000387 DATITW010000000
VERSION     DATITW010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59732"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VLS86_05475"
     CDS             <1..505
                     /locus_tag="VLS86_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007542675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO49976.1"
                     /translation="RIVDVFLSIPELIFALAIAAVLGPSFQNIILALAVVFWVKYARI
                     MRAQIIHVKENEYIDAARISGDSQWRIYFRDILPNSITPVMVQATLDMGHIVLVGATL
                     SFIGLAEAGLAEWGVLVSEGQTGISAGRWWASTFPGLMVFIWALAFNLVGDGLRDVLD
                     PRTETSR"
     gene            502..>1517
                     /locus_tag="VLS86_05480"
     CDS             502..>1517
                     /locus_tag="VLS86_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO49977.1"
                     /translation="MTLPGQTTAVPASSTAPTIAEVRNLKVHFKTKAGIVHAVDGVDF
                     TINDGETLGLVGETGCGKSVTARSFLRLVPMPPGIQVDGSIFFRPRRICPGCAGGGCV
                     TCGFTGRVGAPCPSCSGTGCPSCGQSGIEQVDLLTVPENRLRQIRGDRIAMIFQDPGK
                     ALNPSMSIRQQVGEVFLQHRTDDVLINAGLDPEQASSLQERHASLQSRAGEKAILALP
                     PFRGKRNRINKAVDQMIVAALAETQIPNPLKVMDRYPHELSGGMKQRVMIAQALACNP
                     DLLIADEPTTALDVTVQARIIELIRELQARTRTAVLYISHDLSLVRRVCDRVAVMYAG
                     RIVE"
BASE COUNT          257 a          489 c          494 g          277 t
ORIGIN      
        1 gcggatcgtg gacgtcttcc tctcgatccc ggagttgata ttcgccctgg ccatcgccgc
       61 cgtgctcggg ccgtcgttcc agaacatcat cctggccctg gccgtcgtct tctgggtcaa
      121 gtacgcccga atcatgcggg ctcagatcat ccatgtgaag gagaacgagt acatcgacgc
      181 cgcccggata agcggggact cccagtggcg gatctacttt cgcgacatcc tccccaactc
      241 gatcacgccg gtgatggtcc aggcaaccct cgacatgggc cacatcgtcc tggtgggggc
      301 gacgctgagc ttcatcgggc tcgccgaggc cggtcttgcc gaatgggggg tgctggtgtc
      361 ggagggtcag accgggatct cggcaggacg gtggtgggcg tccacctttc ccgggctgat
      421 ggtcttcatc tgggccctgg ccttcaatct ggtcggtgac ggcttgcgcg atgtgctcga
      481 cccgaggaca gagacaagtc gatgaccttg cccggccaga cgaccgcggt gcccgcatcc
      541 agcaccgccc cgaccatcgc cgaggtgcgc aacctcaagg tccatttcaa gaccaaggcc
      601 ggcatcgtcc atgccgtcga cggcgtcgac ttcacgatca acgatggtga gacgctcggt
      661 ctcgtcggcg agaccggatg cggcaagagc gtgaccgcca ggagctttct ccggctggtg
      721 ccgatgcctc cggggatcca ggtggacggc tcgatcttct tcaggccccg caggatctgc
      781 ccggggtgcg ccgggggcgg ttgtgtcact tgtggcttca ccggccgggt gggagcacca
      841 tgcccgtcct gttcggggac tggttgcccg agctgcggcc agagtgggat cgagcaggtc
      901 gacctgctca cggtccccga gaatcgtctt cgccagattc ggggggatcg gattgcgatg
      961 atcttccaag acccggggaa ggcgctgaat ccttcgatgt cgatcagaca gcaggtcggt
     1021 gaggtgttcc tccagcaccg gacggacgac gttctgatca atgccgggct cgaccccgag
     1081 caagcctcct cgctccagga gcgtcacgcc tcgctccaat cccgggcggg cgagaaggcc
     1141 atcctcgccc tccctccatt ccgggggaag cggaaccgga tcaacaaggc agtcgaccag
     1201 atgatcgtgg ccgctctcgc cgagacccag atccccaacc cgctcaaggt gatggatcgc
     1261 tatccgcacg agctctccgg gggcatgaag cagcgggtga tgatcgcgca ggcgctcgca
     1321 tgtaatcccg acctgctgat cgccgacgag ccaacgacgg ctctcgacgt cacggtccag
     1381 gctcgcatca tcgagttgat ccgggagttg caagcacgca ccaggaccgc ggtcctctac
     1441 atcagccacg acctctcact ggtgcggcgg gtgtgcgatc gggtggcggt gatgtatgcc
     1501 ggccggatcg tcgagga
//
LOCUS       DATITW010000388         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_420219, whole genome shotgun sequence.
ACCESSION   DATITW010000388 DATITW010000000
VERSION     DATITW010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420219"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VLS86_05485"
     CDS             complement(<1..776)
                     /locus_tag="VLS86_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HSO49978.1"
                     /translation="MSDAELRLRVLEMLEWAVDQEQGLPLTSADRRGLLNEIASDVLG
                     YGPIDPLLNDPDVTEVMANGPFDIYYEKRGKIILSDIKFVDEVHLRRIIDKIVGQIGR
                     RVDEATPMVDARLPDGSRVNAVVHPLAIGGPFLTIRKFAVDPYQEADLINFGTITQRV
                     ADFSRACVRGRLNILISGGTGSGKTTTLNVMSSYIPGDERIVTVEDAAELQLHQEHVL
                     SLESRPANIEGKGAVSIRDLVRNTLRMRPDRIVVGAVRGA"
     gene            complement(943..2121)
                     /locus_tag="VLS86_05490"
     CDS             complement(943..2121)
                     /locus_tag="VLS86_05490"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSO49979.1"
                     /translation="MAEHNLLVVDKDDEFVAAAKELFEGRLPVARSLDEASQTIASGD
                     LRMLLLGPSFANDESMEQVRNLHNQDPALVLMLVAELITADLLRGAMRSGVSDVIEAP
                     LDEAKIEAAFEQFGHDLLKRHGSARPVPVQAGRSERGSIITVSSAKGGSGKTVVATNV
                     ALLLNRLPDKKVVLVDADLQFGDVCLLLQLEPRFTMVNAAHELHQLDGEMLDSLLTEH
                     PSGLKVLAAPLEPAFADDITTSGLMQMLDILQDTYDYVVVDTASMLDELILSLIEKSD
                     QVLMVVDMDLPSVKNAKLALETLRLLKFSTANIQLVMNRSNSKAKLDNKEIEGALKME
                     IAAAIPSDAAVPNSVNEGRPIVETDPKGKVAKGFESVAELIAGKIPEGTSRGGLFGRK
                     "
     gene            complement(2145..>2327)
                     /locus_tag="VLS86_05495"
     CDS             complement(2145..>2327)
                     /locus_tag="VLS86_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49980.1"
                     /translation="GNDTIFTLEVTPEQAERIAYAFDNGSIWLTLVPADFVEVETSGV
                     IIDNLFGGDLVEDIFG"
BASE COUNT          424 a          712 c          735 g          456 t
ORIGIN      
        1 tcggcgccac gaaccgcccc gaccacgatg cggtcgggcc gcatgcgcag tgtgttgcgg
       61 accaggtcac ggatgctcac cgcgcccttg ccttcgatgt tggccgggcg ggactccagc
      121 gacaggacat gctcctggtg gagctggagc tcggcggcgt cctcgacggt gacgatgcgc
      181 tcatcgcccg ggatgtacga ggacatcaca ttcaacgtcg tggtcttgcc cgagccggtg
      241 ccacctgaga tcaggatgtt gagccgtccc cggacacagg ccctggagaa gtcggccacc
      301 cgctgggtga tggtcccgaa gttgatcagg tctgcttcct gatagggatc gacggcgaac
      361 ttacgaatcg tgaggaacgg tcccccgatt gccagcgggt gaacgaccgc gttgacacgg
      421 gagccgtcag gaaggcgggc atcgaccatc ggggtggcct cgtcgacgcg acggccgatc
      481 tgtccgacga tcttgtcgat gatcctgcgg aggtggacct cgtcgacgaa cttgatgtcg
      541 gagaggatga tcttgccccg cttctcgtag tagatgtcga aggggccgtt ggccatgact
      601 tcggtgacat ctgggtcgtt gagcagaggg tcgatcgggc cgtagccgag gacgtcggat
      661 gcgatctcgt tgagcaatcc gcgacggtcg gcggaggtga ggggaagtcc ctgctcctga
      721 tcgaccgccc actccagcat ctccaggacc cgaagccgca gttcggcgtc ggacatctgg
      781 cggtcataga gggttgggcc caactcctcg acgaccttga agtgaagacg cccacggagc
      841 tcactgagtg catccgaacg attggcgccg atgtcttgag tttcggtcga ccttgcggct
      901 gcgactctct cagatagcga tggcacgact ctttaccttc ccctatttcc gaccgaagag
      961 gccgccccgt gacgtgccct ccggaatctt tccggcaatc agctctgcaa cactctcgaa
     1021 gcccttggcg accttgccct tgggatcggt ctcgacgatc gggcgaccct cgttgaccga
     1081 gttcggcacc gcggcgtccg aagggatggc cgccgcgatt tccatcttga gcgccccctc
     1141 gatctccttg ttgtcgagct tggccttcga gttgctgcgg ttcatgacca actggatgtt
     1201 ggccgtcgag aatttgagga gacgcaaggt ctccagggcc agcttggcgt tcttcaccga
     1261 tggcaggtcc atgtcgacga ccatcagcac ctgatcggat ttctcgatga gtgacaggat
     1321 cagctcgtcg agcatcgagg cggtgtcgac caccacgtag tcgtaggtgt cctgaaggat
     1381 gtcgagcatc tgcatcagac cggaggtggt gatgtcgtcg gcgaacgccg gctcgagtgg
     1441 cgcggccagc accttcagcc cgctcgggtg ctcggtgagc aacgagtcga gcatctcgcc
     1501 atccagctgg tgcagctcgt gagccgcgtt caccatcgtg aagcggggct ccaactgcaa
     1561 taggagacag acatccccga actggaggtc ggcgtcgacc aggacgactt tcttgtccgg
     1621 cagtcggttg agaagcagag cgacgttggt tgcgaccacc gtcttcccgc tcccaccctt
     1681 ggccgaactc accgtgatga tcgagccccg ctccgagcgg ccggcctgga ccggcaccgg
     1741 ccgggccgac ccatgtcgct tgaggaggtc atggccgaac tgctcgaatg cggcttcgat
     1801 cttggcctcg tcgagtggcg cctcgatgac gtctgagacg ccggatcgca tcgctccacg
     1861 caacaggtcg gccgtgatca gctcggcgac cagcatcagg acgagtgccg gatcctggtt
     1921 gtgcaggttt cgcacctgct ccatcgactc gtcgttggcg aacgacgggc cgagcaggag
     1981 catccgcaga tcgccggagg cgatggtctg actcgcttcg tctagggagc gagcaaccgg
     2041 aagccggccc tcgaagagct cttttgccgc ggcgacgaat tcatcgtctt tgtccacgac
     2101 cagcaagttg tgctcagcca ttgacatggt cgaacctcaa tcaatcatcc gaaaatatct
     2161 tcaacgaggt cgccaccgaa gaggttatcg atgatgacac cggacgtctc gacctcgacg
     2221 aagtcggcag gcaccaacgt caaccagata gaaccattgt cgaacgcgta tgcgattcgc
     2281 tcggcctgct ccggggtcac ctcgagggtg aagatggtgt cgttccc
//
LOCUS       DATITW010000389         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_139612, whole genome shotgun sequence.
ACCESSION   DATITW010000389 DATITW010000000
VERSION     DATITW010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139612"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VLS86_05500"
     CDS             <1..367
                     /locus_tag="VLS86_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49981.1"
                     /translation="DHGGRGNDDDDDTTSTTIEDHGGRGNDDDDTTSTTIDDDKPSAA
                     ITTDPRTYEVDGAGTVTVQVVAGKLVLLDVGVVSGWASEVDEASADKVRVKFRNGSAE
                     AEFEAEFESGKVKIEIERG"
     gene            complement(342..1640)
                     /locus_tag="VLS86_05505"
     CDS             complement(342..1640)
                     /locus_tag="VLS86_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSO49982.1"
                     /translation="MRRRLAILSLATTALVVISFLIPLGLLVRRQASDRARLEAERTA
                     QSTLIALTLTLDSDAAAIEAALGDLEPGTTVFVSTGARFGEAIPGQGSLVEDAMSRQQ
                     ALSGLVDGGWELALPVIGRANTAVVDVFVTDQELTEGVTAAWLLLMLLGVLLVGVAVW
                     VADRLGQSLVRPISDLATTARRLGDGDLDARVKPSDPEEIRDVGESFNWLAGRLEELL
                     VEEREAAADLSHRLRTPLTSLRLQAEKLTDPAERQEVLGQVDRLEQAIDQMILATRSE
                     RTTGVVSCDLSSVVKTRCLFWGVLAEEQGRSVSLNIDPSPVEVHLSSDAVEAMVDALI
                     GNVFSHTLPGTGFAVTTRSDGGRPLLEVSDEGPGFRSAGLANRGVSGAGSTGLGLDIV
                     RRTAELAGGSMEINDGPEGGAVVRVWLGPGGSISRARSRS"
     gene            complement(1637..>2284)
                     /locus_tag="VLS86_05510"
     CDS             complement(1637..>2284)
                     /locus_tag="VLS86_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSO49983.1"
                     /translation="IRRLVAEALANQGHDVESAAVALDGLQLAVKGNPDLVIMDLGLP
                     DLDGTELLRMIRAVSQVPIVVITARGADEMVVRTLDSGADDYLVKPFSVAQLEARVRA
                     VLRRGTSSDPDGEIRVAGLSIDPASREATLDGDLLDLSPKEFDLLRVLAERVGEVVAK
                     RELLAEVWREPYGGSDRTVDVHLSWLRNKLGETAQEPKYLRTVHGVGVKLVDPTR"
BASE COUNT          414 a          814 c          714 g          342 t
ORIGIN      
        1 cgaccacggc ggccgcggga acgacgatga cgacgacacc accagcacca ccatcgaaga
       61 ccacggcggc cgcgggaacg atgacgacga caccaccagc accaccatcg acgacgacaa
      121 gccgtcagcg gcgatcacga ccgaccccag gacctatgag gtcgacgggg cgggaaccgt
      181 gaccgtccag gtggtggccg gcaagctggt gctcctcgat gtcggcgtcg tctccggatg
      241 ggcttcggag gtcgacgagg ccagcgcaga caaggtgagg gtcaagttcc gcaacggctc
      301 cgccgaagcg gagttcgagg ccgaattcga gagcggcaag gtcaagatcg agatcgagcg
      361 cggctgattg acccgcccgg tcccaaccac acccgaacga ccgcccctcc ctcggggccg
      421 tcgttaattt ccatggaacc gccggccaac tccgccgttc tgcgcacgat gtcgagcccc
      481 aggcccgtgg agccggcccc actgaccccg cggttggcga gaccggccga tcggaacccg
      541 gggccttcgt cgctcacctc cagcaggggc cggcccccat ccgatctagt ggtcaccgcg
      601 aagccggtgc cggggagggt atgggagaac acgttcccga tcagagcatc gaccatggcc
      661 tccactgcgt ctgacgacag gtgtacctcg accggcgacg ggtcgatgtt cagcgacacg
      721 ctgcgcccct gctcctcggc caacaccccc cagaacagac agcgcgtctt caccacgctc
      781 gagagatcac acgagacgac tccggtggtg cgctcggagc gggtggcgag gatcatttga
      841 tcgatcgcct gctcgagacg gtccacctga ccgagcacct cctgtctctc ggccggatcg
      901 gtcagcttct ctgcctgcaa ccggagcgaa gtcagtgggg tgcggagtcg gtgggacagg
      961 tcggctgccg cctcgcgctc ctcgacgagc aactcttcga gacgcccggc cagccagttg
     1021 aaagactctc cgacatcccg gatctcctca gggtcactcg gtttgaccct ggcatccaga
     1081 tcaccgtcac caagacgtct cgcagtggtg gccaggtcgg agatcggtct caccaggctc
     1141 tggccgagac ggtcggcgac ccagaccgcc acgcctacca gcaacacacc cagcagcatc
     1201 agcagcagcc aggccgcggt caccccctcg gtcaactcct ggtcggtgac gaacacgtcc
     1261 accacggcgg tgtttgccct gccgatcacc gggagtgcca gttcccagcc tccgtccacc
     1321 aagccactca gggcctgctg tcgggacatc gcgtcctcga ccagcgagcc ttggcccggt
     1381 atcgcctcgc cgaacctcgc gccggtcgag acgaagaccg ttgtccctgg ctcgagatcg
     1441 cccaacgctg cctcgatggc cgctgcgtcg gagtcgaggg tcagagtcaa cgcgatcagg
     1501 gtggactggg cggtgcgctc cgcctcgagc cgggcacggt cggaggcctg gcgtctcacc
     1561 aggagcccga gcgggatgag aaacgagatg acgaccaggg cagtggtcgc caagctgagt
     1621 atggcgagac gccgtctcac ctggtggggt cgaccagctt gacgcctact ccatgcacgg
     1681 tccgcaagta cttgggctcc tgggccgtct cgccgagctt gttccgcagc caggagagat
     1741 gaacgtccac cgtccggtcc gagcctccgt aaggctcgcg ccagacctcg gcgagcaact
     1801 cccgcttggc gaccacctct cccacccgct ccgccagcac ccggagaaga tcgaactctt
     1861 tggggctgag atcgagcaga tccccatcga gcgtggcctc acgcgaggcc gggtcgatgc
     1921 tcagccccgc cacccttatc tcaccgtccg gatcggagga cgtcccacgg cgaaggaccg
     1981 ctctcacccg cgcctcgagc tgggccacgg agaacggctt caccaggtaa tcgtcggccc
     2041 ccgaatcgag ggtgcgcacc accatctcgt ctgcgcctcg ggctgtgatc acgacgatcg
     2101 gcacctggct caccgcccgg atcattctca agagctcggt gccgtcgaga tcgggcaggc
     2161 cgagatccat gatgaccagg tccgggttgc ccttcaccgc gagctggagc ccgtccaggg
     2221 cgaccgcggc gctctcgacg tcgtgcccct ggttggcaag ggcctcggca accagacgtc
     2281 tgat
//
LOCUS       DATITW010000390         1684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_259865, whole genome shotgun sequence.
ACCESSION   DATITW010000390 DATITW010000000
VERSION     DATITW010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259865"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VLS86_05515"
     CDS             complement(<1..476)
                     /locus_tag="VLS86_05515"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HSO49984.1"
                     /translation="MSDALDLWDYRRQVEEIYRKVRQGGVGEETWSAWVSDRNHLFAT
                     HPQSPIEDRSLFTGLPYFPYDPAWRVNARFTAVDDETAILDHSGDGTTRFSKIGGVEF
                     ELMGNPASLELQWLDAYGGGVFLPFRDASNGESTYGGGRYLLDTVKGADLGHDGDS"
     gene            775..1089
                     /locus_tag="VLS86_05520"
     CDS             775..1089
                     /locus_tag="VLS86_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HSO49985.1"
                     /translation="MNVYLEQLQELGRCSQEGSHELFFSEKPSELAEAQRICGGCAVR
                     VQCLEVALRDRHEWGVWGGVIFWDGQPYHRRRGRGRPRQSEANLPIEANTGELWEQVK
                     SA"
     gene            complement(1094..>1684)
                     /locus_tag="VLS86_05525"
     CDS             complement(1094..>1684)
                     /locus_tag="VLS86_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSO49986.1"
                     /translation="EKFEYRKGFKFSTYATWWIRQAITRANADKSRTIRVPVHMMDTI
                     TQMQTAEGNLNRRLGRKPTDEEVSEESGLTVEKIREARKVAPEPVSIFEPVGDDNAML
                     GDFIEDHDAAAPFELIASKLNIENLQFVLERLNERERTIIEMRYGLGNQEPATLDEVG
                     RHFNVTRERIRQIEAKALAKMPHPSTPGGFRELAVS"
BASE COUNT          282 a          513 c          565 g          324 t
ORIGIN      
        1 atgctgtcgc cgtcatgccc caggtcggcg cccttgaccg tgtccagcag atagcgacca
       61 ccgccgtagg tcgactcccc gttgctggca tcccggaacg ggagaaagac ccctccgccg
      121 taggcgtcga gccactgcag ctcgagtgag gccgggttcc ccatgagctc gaactcgacg
      181 cccccgatct tggagaatcg agtcgttccg tcaccggagt ggtcgaggat ggcggtttcg
      241 tcgtcgaccg ccgtgaatcg ggcgttgacc cgccacgctg ggtcgtaggg gaagtagggg
      301 agaccggtga acagcgatcg gtcttcgatc ggcgactggg ggtgggtggc gaacaggtgg
      361 ttgcggtcgc tcacccaggc ggaccaagtc tcctcgccca cccctccctg gcgcaccttg
      421 cgatagatct cctctacctg ccgccggtag tcccagagat cgagagcgtc gctcatggcc
      481 ccaaactagg gcgcctggtt aattgccttg acggatgccc gccgacaggc caatctggcg
      541 ccgtcgagca cagaggtaag caatggtgaa gcagcggatc atgattccca ccggaatggc
      601 agcgcttttt ggcgcgcccg cggctgctcc tgccggcgca gtgccaaaca ccaactggcg
      661 gcctctcgtc acatgatgtg acatcccagc aactttctgt tgtgaggccg ccggacccac
      721 cggcggcttt gtgttttccg cctcccggac agcgacagat caaaggattc gaccgtgaac
      781 gtctatttgg aacaactaca agaactcggg cgatgtagcc aggaaggcag tcatgagctg
      841 ttcttctcgg agaagccttc cgagctggcg gaggcacagc ggatctgtgg tggctgcgcc
      901 gtcagagtcc agtgtctcga ggtcgcgcta cgcgatcgtc acgagtgggg ggtatggggc
      961 ggggtcatct tctgggatgg ccagccctac caccggcgtc ggggccgggg tcgtccccgt
     1021 cagtcggagg cgaatctgcc catcgaggcg aacactggag agctctggga gcaggtgaag
     1081 tcggcctgag agatcaggag acggccagct cccggaagcc tccgggagtg gaaggatgag
     1141 gcatcttggc cagagccttg gcctcgatct gccggattcg ctcccgggtc acgttgaaat
     1201 ggcgtccgac ctcgtccagg gttgccggct cctggttgcc caggccgtag cgcatctcga
     1261 tgatcgtgcg ctcccgctcg ttgagccgct cgaggacgaa ctgcaggttc tcgatgttga
     1321 gcttggaggc gatcagctcg aagggagccg ccgcgtcgtg gtcctcgatg aagtcgccga
     1381 gcatggcgtt gtcgtcgccg accggctcga agatggaaac cggctccggg gcgaccttgc
     1441 gcgcttcgcg gatcttctcc acggtgagcc ccgactcctc ggacacttcc tcgtcggtgg
     1501 gcttgcggcc aagacgacgg ttgaggttgc cctcggcggt ctgcatctgg gtgatggtgt
     1561 ccatcatgtg gaccgggacc cggatggtgc ggctcttgtc ggcgttggcg cgggtgatcg
     1621 cctgacggat ccaccacgtg gcgtaggtgg agaacttgaa gcccttgcgg tactcgaact
     1681 tctc
//
LOCUS       DATITW010000391         1864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_259895, whole genome shotgun sequence.
ACCESSION   DATITW010000391 DATITW010000000
VERSION     DATITW010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259895"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..913)
                     /gene="phoU"
                     /locus_tag="VLS86_05530"
     CDS             complement(200..913)
                     /gene="phoU"
                     /locus_tag="VLS86_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HSO49987.1"
                     /translation="MPETRQRFSEELRALEHEVQQTASQAQLLLEQALQALPGGDLAF
                     CDHLIRGDDDVDALYLDVERRILHLFALQTPVATDLRLLTALLHINLHLERVGDQAVN
                     LAKIAKLVDGLPQIPEVLRDLEEMGKIALGMVGTAMDALARRDVDLAQTLPELDDPID
                     QLNRGMMPKVLEASGDKRMLEWGIRMYVVSRQIERIGDNAVDIGEQVAFLVTGQFREF
                     TDASHPEVEHPELFKDGPS"
     gene            1263..>1864
                     /locus_tag="VLS86_05535"
     CDS             1263..>1864
                     /locus_tag="VLS86_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HSO49988.1"
                     /translation="MLAAACGGGNDDGGSADGGSQLSGTINIDGSSTVAPLSEAAAEL
                     FQEQNSGVRVTVGTSGTGGGFEKFCAGETDISDASRAIEADETAACESKGIKYEEVQV
                     ANDGLSVVVNNQNTWANCLTTAQLKAIWDKGSKVNNWNQVDPSFPNEPLKLFGAGTDS
                     GTFDYFTNAINGEEGRSRSDYSATEDDNVTVTGVSGTKGGL"
BASE COUNT          338 a          590 c          613 g          323 t
ORIGIN      
        1 ggggccgttt gccagagatt tgccagaatc tcaccacacg cgactgttaa tcactctgag
       61 cgttgaccgg ttcggtggat ttcggcgttc tcaggacgca cgccttcgta aggactttcc
      121 ccacaagtca aggccgtcct cggccggcag ccgccacctt cgtgccaccc ggcaccaggc
      181 ctcattcagg cgggccagcc taactgggtc cgtccttgaa gagctccggg tgctcgacct
      241 cgggatgaga tgcgtcggtg aactcacgga actggcccgt gacgaggaat gcgacctgtt
      301 cgccgatatc taccgcgttg tcaccgatcc gctcgatttg acgcgacacg acatacatgc
      361 ggattcccca ctccagcatc cgcttatcgc cggaggcctc aagcaccttc ggcatcatcc
      421 cgcggttcag ctggtcaatg gggtcgtcaa gctcgggcag cgtctgggcg aggtcgacat
      481 cacggcgcgc caaagcgtcc atcgccgtgc ccaccatgcc gagggcgatc ttgcccatct
      541 cttccagatc ccgaaggacc tctgggatct gggggagacc gtcgaccagc ttggcgatct
      601 tggcgaggtt cactgcctgg tcgccaacgc gctccagatg caggttgatg tgcagcaggg
      661 cggtgaggag ccgcaggtcg gtcgcaactg gggtctggag cgcgaacagg tgcaggatgc
      721 gtcgctcgac gtccagatag agagcgtcca cgtcgtcatc gcccctgatc aaatggtcgc
      781 agaacgccag gtcgccaccc gggagggcct gtagcgcctg ttccagcagc agctgggcct
      841 gggaggctgt ctgttgcacc tcgtgctcaa gggcgcgcaa ctcttccgag aagcgttggc
      901 gagtctcggg catcagccag ccctcctgtc ctgtagggct cgggccgaga gccgatggac
      961 ataacaagca ttgcccggta ggtcctaccc caatcagccg gaccccgcgc cagcctcctc
     1021 catgggcagg gccaccggct gtcgctcaac ggccaggacg cgcctgacat tctctctttg
     1081 ttcacccgct gttcatgtgc cgtcgagagc gacgtcaagg accgtggcta ccgtgcgcgt
     1141 cgtatggagc gactctgcga tggacagggg cccatgctct tcgagcgtga gggaggacaa
     1201 cccgtatgac cggacgactc gtggcgcgct ggcgctatgg tgcgctgctg ctcgccatcg
     1261 ggatgttggc ggccgcatgt ggcggcggca acgacgatgg tgggtccgcc gatggcgggt
     1321 cccagctgtc ggggacgatc aacatcgacg gctcgagcac ggtggccccg ctgagcgagg
     1381 ctgctgctga gctgttccag gagcagaact cgggcgtccg ggtgacggtg gggacctcgg
     1441 gcaccggcgg tgggttcgag aagttctgcg ctggcgagac cgatatctcg gatgcgtccc
     1501 gggcgatcga ggccgacgag accgcggcct gtgagagcaa gggcatcaag tacgaggagg
     1561 tccaggtcgc caacgacggg ctgtcggtgg tggtcaacaa ccagaacacc tgggccaact
     1621 gcctgaccac cgcgcagctc aaggcgatct gggacaaggg cagcaaggtc aacaactgga
     1681 accaggtcga cccgagcttc cccaacgagc ccctgaagct gttcggtgcc gggaccgact
     1741 cgggcacctt tgactacttc accaacgcga tcaacgggga agagggccgc agccgctcgg
     1801 actactcggc caccgaggac gacaacgtga ccgtgaccgg cgtgagtggc accaagggtg
     1861 gcct
//
LOCUS       DATITW010000392         1729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_500083, whole genome shotgun sequence.
ACCESSION   DATITW010000392 DATITW010000000
VERSION     DATITW010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500083"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1016)
                     /locus_tag="VLS86_05540"
     CDS             complement(12..1016)
                     /locus_tag="VLS86_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014913184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO49989.1"
                     /translation="MTTPLVEVEDLHVHFGEARAVDGASLQVAPGEVLALVGESGSGK
                     TTLIRAIQGLQKPTSGEIRVEGSPIDNRSRRQIQTIFRNPAVQMVFQDPTGALNPRQT
                     IYEIVAEGLRIHGRPERESERVAEALSRAGLRPPDRFFQFYPHEISGGQRQRVVIAGA
                     MVMEPRLLLADEPVASLDASIRGEILALMRHLVDDSGLAILVVTHDLGLAWNIADRVA
                     VMYLGRIVETGPTEEILGDPKHPYTKALLSVVPETREMEQQILVGEKPDSARIPAGCR
                     FHPRCPLLQSGTLPAEVAARCTADDPGPVAASALSAAAGGAVSLPADFGPDQPSGLEG
                     "
     gene            complement(1013..>1729)
                     /locus_tag="VLS86_05545"
     CDS             complement(1013..>1729)
                     /locus_tag="VLS86_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO49990.1"
                     /translation="GLRAVRWTSGAIIFQGALHALNPVQTVGDQIAEPMLVHETAASE
                     TVARERAIDLLRRVGLPAARADSYPHQLSGGQRQRVLIAMALACDPHLLIADEPTTAL
                     DLMVQAQVLALLDDLKQERELTLMFITHDLSVLTYACQRLAIMYAGRIVEEGPSWDVF
                     AGPSHPYTRALAAAFPTIGDVSSRMAPSGLGGDPPTPGNLPQGCPFHPRCPLARDECL
                     PAEPELRSSGIGRSAACILV"
BASE COUNT          292 a          584 c          580 g          273 t
ORIGIN      
        1 tctgaggcgg cctacccctc gagccctgag ggttggtcgg ggccaaagtc ggccggcaga
       61 gaaacggccc cgccggcggc cgcggacagg gcgctggcag ccaccggacc gggatcatcg
      121 gcagtgcacc tcgccgccac ctccgcaggc agggtgcccg actgcagcaa cgggcaccgg
      181 gggtggaacc ggcatcccgc ggggatcctc gccgaatccg gtttctcccc caccaggatc
      241 tgctgctcca tctctctggt ctcggggacg acagagagca gcgccttggt gtaggggtgt
      301 ttcgggtctc ccaggatctc ctcggtaggc ccggtctcga cgatccggcc cagatacatc
      361 accgcaaccc gatcggcgat gttccaggcc agacccaggt cgtgagtcac taccaggatc
      421 gccaaaccgg aatcgtcgac caggtgccgc atcagggcga ggatctcgcc gcgaatcgag
      481 gcgtcgaggc tggccaccgg ctcatcggcc agcagcagcc tcggctccat caccatcgcc
      541 ccggcgatca ccacccgctg tcgctgacca cccgagatct cgtgcgggta gaactggaag
      601 aaccggtccg gtggccgaag accggcccgg gacagggcct cggcgacacg ctcggattcc
      661 cgctccggcc ggccgtggat ccggagccct tcggcaacga tctcgtagat ggtctgtctc
      721 gggttgagag cccccgtcgg gtcctggaac accatctgca ccgccggatt gcggaagatg
      781 gtctgaatct ggcgtctcga tctgttgtcg atcgggctcc cttcgacacg gatctccccc
      841 gatgtcggtt tctgaagacc ctggatggcc cgaatcagag tggtcttgcc cgaccccgac
      901 tccccgacca gggccagcac ttcgccggga gcgacctgga gggatgctcc gtccacggcg
      961 cgggcctcac caaaatggac gtggaggtcc tcgacctcga ccaggggggt ggtcacacca
     1021 ggatgcacgc cgcgctcctt ccgatgcccg agctcctcaa ttccggctcc gccggaagac
     1081 attcatccct ggccagcggg catcggggat ggaacggaca tccctggggc aggttccccg
     1141 gtgtgggggg gtcccccccg agaccggagg gagccatacg cgagctcacg tccccgatcg
     1201 tgggaaacgc agcggcgagg gcgcgggtgt agggatggga ggggccggca aagacatccc
     1261 atgacggtcc ttcttcgacg atccgacccg catacatgat ggccagacgc tggcacgcat
     1321 aggtgaggac cgagaggtcg tgggtgatga acatcagcgt gagctcccgc tcctgcttca
     1381 ggtcgtcgag caaggcgagc acctgggcct gaaccatcag atcgagggca gtggtgggct
     1441 cgtcggcgat gaggaggtgg gggtcgcagg ccagcgccat cgcgatgagg accctctgcc
     1501 gctgaccacc cgacagctga tgggggtacg aatcggcgcg ggctgccggt agcccgaccc
     1561 tcctcagcag gtcgatggct cgctcccggg ccactgtctc ggacgccgcc gtctcatgga
     1621 cgagcatggg ctctgcgatc tggtctccaa ccgtctgcac cgggttgagg gcgtggagtg
     1681 ccccctggaa gatgatggcg ccggacgtcc atcgcaccgc cctcagccc
//
LOCUS       DATITW010000393         1554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_460211, whole genome shotgun sequence.
ACCESSION   DATITW010000393 DATITW010000000
VERSION     DATITW010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460211"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1554
                     /locus_tag="VLS86_05550"
     CDS             <1..>1554
                     /locus_tag="VLS86_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49991.1"
                     /translation="MTALSGAVGSPALYAEVRLRRASAARASEDFDLAAELAQAGCVS
                     AEDSGDRSLELSACLELGQAITKSPIGEGYITAVEVDVDAAEDAYQRALGIAREIGAR
                     SSEADALRELAMIEAGRVKNVAIGLEEGGASKLEILMQASDLFVTAKDLAEQALRIYE
                     EIGDQQGSMSALITLAYAHVADPTAQGMAGRIEHVRALHLSRKGEITESQSAIDEAQG
                     LFAIATYARLNVQPGLALLRGREAFDAARAIGDRWLEALAAGGMAMTCLQVGGDDECV
                     AWLDRAATAAMAVASTSMARRLEMWRGAHAAAVDDVEAMSRHFRRAAELAGQRSPGER
                     CEALANLAFEYARIAALKGDTTLLEPARETANEAMELSRQLKGPVPWGAVAHASLALV
                     AEAEGDSTTAADEARNALDIDGETYLNYFVPILWVAARILIVGDEPEAASLSAQIIAG
                     ISSLGMSLGDPELRDKWFATPMIAEVSRIVGFDPAESLATDEAGAPELNEADLALLRE
                     LASGSSDRSP"
BASE COUNT          252 a          495 c          554 g          253 t
ORIGIN      
        1 gatgaccgcc ctcagcgggg cggtagggtc acccgccctc tatgcggagg tgaggctgcg
       61 acgagcctcg gcggcgcggg ccagcgagga cttcgacctg gctgctgagc tggcccaggc
      121 ggggtgtgtc tccgccgagg actccggtga ccggagtctg gagctgtcgg cgtgtctcga
      181 actgggccag gccatcacca agtccccgat cggtgagggg tatatcaccg cggtggaggt
      241 ggacgtcgat gcggccgagg acgcctacca gcgtgcgctg gggatcgccc gtgagatcgg
      301 cgcccgctcc tcggaagccg atgccctccg cgagctggcg atgatcgagg cggggagagt
      361 gaagaacgtg gcgatcggcc tcgaagaggg cggggcttcc aagctggaga tcctgatgca
      421 ggcatcggac ctgttcgtga cggccaagga cctggccgag caggcgctgc gcatatatga
      481 ggagatcggc gatcagcagg gctcgatgtc ggcgctgatc accctggcct atgcccacgt
      541 cgccgatccg acagcccagg ggatggccgg tcgcatcgag cacgttcggg ccctccatct
      601 cagccggaaa ggggagatca cagagagtca gtcggctatc gacgaagccc aggggctctt
      661 cgccatcgcc acctatgccc ggctcaacgt ccagcccggg ctcgccctcc tacgaggacg
      721 tgaggcgttc gacgcggctc gggccatcgg cgaccgctgg ctggaagctc tggcggccgg
      781 ggggatggca atgacctgtc ttcaggtggg cggcgacgac gagtgcgtcg cgtggctcga
      841 ccgtgccgcg accgcggcca tggcggtggc ctcgacctcg atggcccggc gcttggagat
      901 gtggcggggg gcccacgcag ctgcggtcga cgacgtggaa gcgatgagtc gccatttccg
      961 gcgcgctgcc gagctggccg ggcagaggag tcccggtgag cgatgcgagg ctttggcgaa
     1021 tctcgctttc gaatacgccc gcatcgcggc actcaaaggt gacaccaccc tcctcgaacc
     1081 tgctcgagag actgcgaatg aggcgatgga gctctcccgt cagctgaaag gtccggtccc
     1141 ctggggggcg gtcgcccacg cctctctggc tctggtcgcc gaggcggagg gagattcgac
     1201 taccgcagcc gacgaggccc gcaacgccct cgacatcgac ggggagacct atctgaacta
     1261 cttcgtgccc atcctctggg tggcagcccg gatcctgatc gttggtgacg agccggaggc
     1321 ggccagtctc agcgcccaga tcattgccgg catttcctcc ctgggtatgt ccctcggcga
     1381 ccccgagctg cgagacaagt ggtttgccac cccgatgatc gccgaggttt ccaggatcgt
     1441 tggcttcgac ccggccgaat cactggctac cgacgaggcg ggcgcgccgg agttgaacga
     1501 agctgacctg gctctccttc gtgagctggc ctcgggatcg agcgatcgaa gccc
//
LOCUS       DATITW010000394         1503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_420363, whole genome shotgun sequence.
ACCESSION   DATITW010000394 DATITW010000000
VERSION     DATITW010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420363"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VLS86_05555"
     CDS             <1..394
                     /locus_tag="VLS86_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO49992.1"
                     /translation="PRRPDPETAHAVFDLFPRLAERRRQRAGTRSGGEQQMLAVGRAL
                     MSKPRLLILDEPTTGLAPKLAAEAYHALGILKDRGLTVLVAEQQVPLAMALSNRGYVL
                     ENGVIRKEGTSAELSNDPDVRQAYLGIV"
     gene            391..1248
                     /locus_tag="VLS86_05560"
     CDS             391..1248
                     /locus_tag="VLS86_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSO49993.1"
                     /translation="MTANILDGIVLGLQFALLAAGLTLVYGLGGVLNLAYGQMAVLSA
                     MTVVLSMEAGLPLFPAVIVGLVAGGVLGLVFDRTILRPVYRLRGEARVLLSLLLTLGM
                     ALAVDGFLSWRYPTKALTLRIGGGPVEIAGVRMVRGAVVASAIAIMVLAGLLLFFRKT
                     TLGRAVRSVIEDEEGARLVGVDPHHILRIILTLTGVLAALVAVTRSLVTPVSVTAGFE
                     LTVSALIVTVVGGLGSVGGAILAGVILGIVSTVAASSIGTVISLVILLVAAAVTILIR
                     PSGLLGVRE"
     gene            1245..>1503
                     /locus_tag="VLS86_05565"
     CDS             1245..>1503
                     /locus_tag="VLS86_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO49994.1"
                     /translation="MTDRFIEATGTSGIGGVEHRASRSLFALVWPILALGAVLALVPL
                     WLGGSRVMMGVSVVGLVFACYAIGFNLIFGSTGQLFLCVGAL"
BASE COUNT          202 a          476 c          515 g          310 t
ORIGIN      
        1 cccgcgccga cccgacccgg agaccgcaca tgcggtgttc gacctgttcc cgcgtctcgc
       61 cgagcgacgc cgtcagcgcg ccgggacgag gagtggtggc gaacagcaga tgctcgcggt
      121 ggggcgggca ttgatgtcaa agccccgtct gctcatcctg gacgagccca ccacgggact
      181 ggcgcccaag cttgccgccg aggcgtatca cgcgctcgga atcctcaagg accgggggct
      241 gacggtgctg gtcgccgaac agcaggtccc gctggccatg gcgctgagca atcgtggcta
      301 cgtcctcgag aatggggtca tccgcaagga gggaacctcg gcggaactgt caaacgaccc
      361 ggatgtccgc caggcctacc tggggatcgt gtgaccgcca acatcctcga cgggatcgtt
      421 ctcgggcttc aattcgcact gctcgccgcc gggctcacac tggtatacgg gcttggcggc
      481 gtcctcaatc tcgcctacgg gcagatggcg gtgctctcgg caatgacggt tgtcctttcc
      541 atggaagccg gtcttcccct ctttccggct gtgatagtgg gtctggtggc cgggggggta
      601 ttgggcctcg tcttcgaccg gaccatcctg cgccctgtct accgccttcg gggcgaggcc
      661 agggtcctgc tcagccttct cctgacactc ggaatggccc tcgcagtcga cggcttcctg
      721 agctggcgat acccaaccaa ggcgctcact ctccggatcg gcggaggccc agtcgagata
      781 gccggggtgc ggatggtgag gggtgcagtg gtggcatcgg cgattgccat aatggtgctc
      841 gctgggctgt tgctcttctt ccgcaagacc acgttggggc gggcggtcag gtcggtgatc
      901 gaggacgagg aaggggcgcg cctggtcggc gtcgatcccc atcacatcct tcgcatcatc
      961 ctgaccctga ccggagtgct ggctgcgttg gtggcagtga cccggtcgtt ggtcaccccg
     1021 gtttcggtga ctgcgggttt cgaactgacg gtgtcggccc tgattgtcac cgtggttggc
     1081 gggttgggca gtgtcggcgg cgccattctc gccggtgtca tcctggggat cgtcagcacc
     1141 gtcgcggcct cctccatcgg taccgtgatc tcgctggtca tcctgctggt ggcggcggca
     1201 gtgaccatcc tcatccgtcc ctccggcctg ctcggggtgc gggaatgacc gacaggttca
     1261 tcgaggcgac cggcacctcc ggcatcggtg gggtggagca ccgagcatcg cggtcactgt
     1321 ttgccctggt ctggcccatc ctggccctgg gtgcggtgct cgccctcgtc cctctctggc
     1381 tcggcggctc tcgggtgatg atgggtgtgt cggtggtcgg tctggtcttc gcttgctacg
     1441 ccatcgggtt caacctgatc ttcggaagta ccgggcaact gttcctatgc gtcggggccc
     1501 tgg
//
LOCUS       DATITW010000395         1648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_620667, whole genome shotgun sequence.
ACCESSION   DATITW010000395 DATITW010000000
VERSION     DATITW010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620667"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VLS86_05570"
     CDS             complement(<1..435)
                     /locus_tag="VLS86_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HSO49995.1"
                     /translation="MPTSGSTLGDVSRRVGGHLSGDPATRLTDVTHDSRSAGPGVLFV
                     AVRGSNHDGHDDVEAAVASGSPAVAVEHPVPIDVGQLVVDDTRAVMGAMAAEVHGDPS
                     THVAVVGVTGTNGKTTVTHYIETMLDSAGRLAGLIGTVHTRVG"
     gene            complement(419..>1648)
                     /locus_tag="VLS86_05575"
     CDS             complement(419..>1648)
                     /locus_tag="VLS86_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSO49996.1"
                     /translation="YEGQLRGVPGKAVFERDLEGRIIPQGISDIVPAVPGTDLNTTID
                     LPLQYQAQEACLGAMGRTEAESCWVVVLDVETGGILAMAGAPVFDPQTRQTLDEDCDP
                     DADPTCHSFSNFIVRGVYEPGSTQKLITVAAALEEREVTAGTVIPDVADLLELREDAC
                     RSADDDTYGCYRDFEEHETADMNVAEIFTRSSNVGTILVAEKLGQDRLISYIEKFGLG
                     SATGIDYSVEAPGFLNFEAGCETCWASAAIGYSVAATPLQMAAAYAAIGNDGVWTAPH
                     IVASTTDLDGNEDVTGLETRRVVSEGTASLMQDLLANVVHEGTGQAASVAGYRVGGKT
                     GTANKLGEDGRYTEVTRASFVGMAPMDDPKVVVAVLIDAPSFEYRTGGASAAPVFAQV
                     MEQALHRLGVTPDADLG"
BASE COUNT          264 a          553 c          542 g          289 t
ORIGIN      
        1 cccgacccgg gtgtgaaccg tcccgatcag gcccgccagc cgacctgcgc tgtcgagcat
       61 cgtttcgatg tagtgggtga ctgtggtctt cccattggtc ccggtgaccc cgaccacggc
      121 gacatgtgtc gatgggtcac cgtggacttc tgccgccatc gctcccatga ccgccctggt
      181 gtcgtcgacc accagttggc cgacgtcgat cgggaccggg tgctccacgg cgaccgcagg
      241 agagcctgac gccaccgcgg cttccacatc gtcgtgaccg tcgtgattcg acccacgcac
      301 cgcgacgaaa aggactccgg ggcccgcgct cctcgagtca tgggtcacgt cggtgagccg
      361 ggtggccgga tcaccggata ggtgtccccc gacccgacgc gagacgtcgc cgagagtgct
      421 acccgaggtc ggcatcgggg gtgactccaa ggcgatggag agcctgttcc atgacctggg
      481 caaagacggg agccgccgat gcgcccccgg ttcgatactc gaaggagggc gcgtcgatca
      541 agaccgcgac gacgaccttg gggtcgtcca tgggagccat cccgacgaac gaggcgcggg
      601 tgacctcggt gtatctgccg tcctcgccca acttgttggc ggtgccggtc tttcccccga
      661 cccggtaccc ggcgacggag gcggcctgcc cggttccctc atggacgaca ttggccaaca
      721 ggtcctgcat caacgacgcc gttccctccg agaccacccg tctggtctcc aggccggtga
      781 cgtcctcgtt tccgtcgaga tcggtcgtgg atgcgacgat gtggggggcg gtccacaccc
      841 cgtcgttgcc gatggcggcg taggccgccg ccatctgcag cggcgttgcc gccaccgagt
      901 atccgatggc tgccgacgcc cagcaggtct cacaccccgc ttcgaagttg aggaacccgg
      961 gcgcctcgac cgagtagtcg atgcccgtgg ccgagcccag cccaaacttc tcgatgtagg
     1021 agatgagccg gtcctgtccc agcttctcgg cgaccaggat ggtcccgacg ttggaagagc
     1081 gcgtgaagat ctcggcgacg ttcatgtctg cggtctcgtg ctcctcgaag tcccgatagc
     1141 agccataggt gtcgtcatct gccgaacggc aagcgtcttc ccgaagttcc agcaggtcgg
     1201 ccacgtcggg gatcaccgtc ccggccgtga cctctctctc ctcgagggcg gctgccaccg
     1261 tgatcagctt ctgggtggag ccgggctcgt agacaccgcg gacgatgaaa ttcgagaacg
     1321 agtgacacgt cgggtcggca tcagggtcgc agtcctcgtc cagggtctgg cgggtctgtg
     1381 ggtcgaacac cggggcaccg gccatggcga ggatcccccc ggtctccacg tcgaggacga
     1441 ccacccagca cgactcggct tcggtccggc ccatggcccc gagacaggcc tcctgtgcct
     1501 ggtactgcag tggcagatcg atcgtggtgt tgagatcggt gccgggcact gcagggacga
     1561 tgtcgctgat gccctgaggg atgatgcgac cctcgaggtc gcgttcgaag acagccttgc
     1621 cgggaacacc gcggagctgc ccttcgta
//
LOCUS       DATITW010000396         1684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_139868, whole genome shotgun sequence.
ACCESSION   DATITW010000396 DATITW010000000
VERSION     DATITW010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139868"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VLS86_05580"
     CDS             complement(<1..575)
                     /locus_tag="VLS86_05580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSO49997.1"
                     /translation="MSTQLTTAELALQARARAFVDELIPWEVEAEMNRGEIPEVVSKA
                     QKEKARELALTSINMSERLGGPGYGMLAQTLIQEQTGRVTNALGWVVSTGPSWIEEVA
                     TDHQIETWIRPTVDGDIHECYAITEENAGSDVDGIEATARRDGDDYILNGTKWHVTSA
                     NLASYVFFQAKLSEGPNSGRHVLFFVDMDTPG"
     gene            complement(582..>1684)
                     /locus_tag="VLS86_05585"
     CDS             complement(582..>1684)
                     /locus_tag="VLS86_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSO49998.1"
                     /translation="AGWATKVMGSIPPVGPEAMSLVVKEPVGVAALITPWNYPILMAS
                     QKVAPALAAGCTTILKPAEQTPMTAIELARIIDEAGAPPGVFNLLTGYGPDAGAPLVS
                     HPGVDKVSFTGSVEVGKIISRSASDTLKRVTLELGGKSPNLIFSDADFAAAIEGTCSG
                     IFANSGEICSAGSRVLVERSIYAEAVSSMIDGASTIKLGSGLDPETTMGPVISFEHRD
                     TVLAYIGSGRESASVAYEGIVPTDPALAGGYFVAPTIFTEVGNDTLIAREEIFGPVMA
                     VIPFSDEEEALRIANDTTYGLAAAIWTSDVKRAMRIARGIRAGVVWVNDSQPAPTESI
                     WGGYKTSGVGRELGPWGIESYLEEKQIYLNLA"
BASE COUNT          287 a          559 c          525 g          313 t
ORIGIN      
        1 cccggggtgt ccatgtccac gaagaagagg acgtgtctcc ccgagttggg gccttcgctc
       61 agcttggcct ggaagaacac atacgaggcg agattggccg aggtcacgtg ccatttcgtc
      121 ccattgagga tgtagtcgtc gccgtcccgg cgtgctgtgg cttcgatccc atcgacatcg
      181 gagcctgcgt tctcctcggt tatggcatag cactcgtgga tgtcaccgtc gacggtgggg
      241 cggatccagg tctcgatctg gtgatcagtg gccacctcct cgatccacga tgggccggtc
      301 gagaccaccc agcccagggc gttggtgacc cggccggtct gttcctggat cagggtctga
      361 gccagcatgc cgtaaccggg gccgcccagt cgctcggaca tgttgatcga ggtcaacgcc
      421 agctccctgg ccttttcctt ctgcgccttc gacaccacct cggggatctc gccccggttc
      481 atctcggcct cgacctccca ggggatgagc tcgtcgacga aggcccgcgc ccgagcctgg
      541 agcgccaact cggcagtggt caattgggtg ctcatgttct cctaggccag attgagatag
      601 atctgctttt cctcgagata ggactcgatc ccccacgggc cgagctccct gcccacaccc
      661 gacgtcttgt agccgcccca gatcgactcg gtgggcgccg gctggctgtc attcacccag
      721 accacgccgg cccggatccc tctggcgatc cgcatcgcac gcttcacgtc agaggtccaa
      781 atcgccgccg ccagcccgta ggtcgtgtcg ttggcgatac gaagagcctc ttcctcgtcg
      841 gagaagggga tcaccgccat caccgggccg aagatctcct cgcgggcgat gagagtgtcg
      901 ttgcccacct cggtgaagat ggtcggggcg acgaaatagc caccggccag cgcgggatcg
      961 gtgggaacga tgccttcgta ggccaccgac gccgattccc gacccgatcc gatgtacgcc
     1021 agcaccgtgt cccggtgctc gaatgagatc accggtccca tggttgtctc agggtcgagc
     1081 ccgcttccca gcttgatcgt cgacgccccg tcgatcatcg acgagacggc ttcggcgtag
     1141 atcgatctct cgaccaagac ccggctcccc gcggagcaga tctccccgga gttggcgaag
     1201 atgccggaac aggtgccctc tatggctgca gcgaagtcgg catcggagaa gatcagattc
     1261 ggcgatttgc cgccaagctc gagtgtcacc cgcttcaagg tgtcggaggc cgaccgggag
     1321 atgatcttgc ccacctccac cgatccggtg aaggaaacct tgtccacccc tggatgggag
     1381 acgagcggag ccccggcgtc cgggccgtat ccggtgagca ggttgaacac gcccggagga
     1441 gcgcccgcct cgtcgatgat ccgggccaac tcgatggccg tcatcggtgt ctgttcggca
     1501 ggcttgagga tggtggtgca ccccgcggcg agagcaggag ccaccttctg gctggccatc
     1561 aggatcgggt agttccaggg agtgatcaga gccgccaccc cgaccggctc cttgaccacc
     1621 aagctcatcg cctcggggcc gaccggagga atcgagccca tgaccttggt cgcccagcct
     1681 gcgt
//
LOCUS       DATITW010000397         3731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_780714, whole genome shotgun sequence.
ACCESSION   DATITW010000397 DATITW010000000
VERSION     DATITW010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780714"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VLS86_05590"
     CDS             <1..619
                     /locus_tag="VLS86_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011900664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HSO49999.1"
                     /translation="PSGAGIGAHEHHCDSPEAYFVHAPGLVVVCPSTPIEAKGLLASA
                     MTGEDPVIFLEPKVLYRAGREEVPIEHYTLPIGRARVRREGTDLTLVTYGGMVPVALE
                     AADQVSESIEVIDLRTLFPWDRETVLASVRKTGRLLLVQEPQGSAGVAADVAAVVAEK
                     ALYDLEAPIVRVTGFDVPWPQFAIEKHALIDPQRVIAGIREALGA"
     gene            631..1182
                     /locus_tag="VLS86_05595"
     CDS             631..1182
                     /locus_tag="VLS86_05595"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HSO50000.1"
                     /translation="MAVFGSSQTDPGTNDWEDAERAGRRLAQAGLDVVTGGYGGTMEA
                     VSRGAAEAGGHVIGITAPTLFPARSGANPFVSELIEASSLAHRIDLMMQRATATLALP
                     GSIGTAAELLISWNTNHIRRRSGGALFPAAAVGPGWKTVGRALVTAIGAVEGDIYWAD
                     SADDGVDWLLSTGLGTPSGQRTT"
     gene            1299..2177
                     /locus_tag="VLS86_05600"
     CDS             1299..2177
                     /locus_tag="VLS86_05600"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSO50001.1"
                     /translation="MTRLIRAAGGVVFRKTAKGNLRVLVAHRPQYDDWSLPKGKCDPG
                     ETPEVTAVREVLEETGSHCRIVAPLGVTRYRVTGGVKEVSWFAMKPLPDSPGFAENRE
                     VDSIKWLSRRRAAELLSYDHDRDLVQETDIKKLSQTGILRLLRHSTAGDRTKWKGQDR
                     NRPLTKKGRSQAESIALSLADAGIERIVTSPYDRCLQTVEPLSRVTGARIEISDTLAE
                     EAELDPAYALVDGLVGSNAVICSHGDVIPALINRMMWAGLSLTSRFYCSKGSIWEVDV
                     DGGKFTTARYVPPPDS"
     gene            complement(2185..>3731)
                     /locus_tag="VLS86_05605"
     CDS             complement(2185..>3731)
                     /locus_tag="VLS86_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50002.1"
                     /translation="VALAVGVVVARASVLRLIKSSSTGLSDLRTLTFRHLLRRSVLHV
                     DADRRGDLVSRVTSDVTTLQEFMEWGGVSMVIATSQVVLALGVMVYYEWRLALVAVLG
                     VVLYLLLMRWFQNVLARNYDHVRTEVGSSLGVLSESISAIPVVRAYGIEGPTTSRVAD
                     ALERRFWIEFRTARLGNILFSSAEVFAGALTAAVVVGGIIIGTSQGMTAGTLVAFLFL
                     TNLLIEPLQIVVETLDFAQSAGSGLRRILGVLDTEPEMVDPEDPDHLHVGGLSARFQD
                     VGFSYPTSGEVLSGVTVDIPVGSRVAVVGETGSGKTTFAKLLVRLLDPTQGNVWIGGV
                     NARKLPLAELRDRVAFVPQEGFLFDDTIANNVRYGRPEASDAEVWTAFHELGLGVWVE
                     GLADGLDTRVGERGGNLSAGERQLVALVRAWIGSPDLLVLDEATSAVDPALDVALRRA
                     IERLIEGRTSVTLAHRPATAEGSDEVLVFDQGVLVERGHHRELLARGGTYAGLYADWT
                     TGTKTV"
BASE COUNT          677 a         1266 c         1219 g          569 t
ORIGIN      
        1 ccccagcgga gccgggatcg gagcccacga gcaccactgc gactcccccg aggcctactt
       61 cgttcatgca ccgggcctgg tcgtggtgtg cccttcgacc ccgatcgaag ccaagggcct
      121 gctcgcctcg gccatgaccg gtgaggaccc cgtgatcttc ctcgagccca aggtcctcta
      181 ccgggcaggg cgtgaggaag tgccaatcga gcattacacc ctgcccatcg gcagggcgcg
      241 ggtgcgacgg gagggcaccg acctcaccct ggtcacgtat gggggcatgg tcccggtggc
      301 gctggaggcg gcagaccagg tgagtgagtc gatcgaggtc atcgatctcc gcaccctgtt
      361 tccctgggac cgggagacgg tcctggcctc ggtgcggaag accggcagac tgctcctcgt
      421 ccaggagccc caggggtctg ccggggtggc cgccgacgtg gcggcggtgg tcgccgagaa
      481 ggccctctac gacctcgagg cgccgattgt gagagtcacc ggcttcgacg tcccctggcc
      541 tcagttcgcc atcgagaagc acgccctcat cgatccacaa cgtgtgattg ccggcatccg
      601 ggaggcactg ggcgcttgag ccggccgacg gtggccgtct tcggctcctc gcagaccgac
      661 ccgggaacca acgactggga agatgcggag cgggcagggc ggagactggc acaagcgggg
      721 ctcgatgtcg tgaccggtgg atacggaggg acgatggaag cggtttcgcg cggggcagcc
      781 gaggccggag gccatgtgat cggcatcact gctccaaccc tgtttcccgc cagatccggg
      841 gccaacccgt tcgtgtcgga gttgatcgag gcttccagcc tcgcccaccg gatcgacctg
      901 atgatgcagc gggcaactgc gacgctggcg ttgcccggct cgatcgggac cgccgccgag
      961 ctgctcatat cgtggaacac caaccacatc aggcgccgca gcggcggcgc tctctttccc
     1021 gcggcggcgg ttgggcccgg atggaagacc gtcggtcggg ccctggtgac tgccatcggc
     1081 gccgtcgagg gcgacatcta ctgggctgac tcggcggacg acggtgtgga ctggctgcta
     1141 tccaccgggc tcgggacgcc ttccgggcaa agaaccactt gacttccaga gtggggccgt
     1201 gacctcagac ctgaacatgg gccatctcct caccggatca gggcgcctgg gtcggcggcg
     1261 cacgcgacaa aacagggacc agccctaagc tccccgccgt gacccgactc atccgagccg
     1321 ccgggggtgt cgtcttccgc aagacggcga agggcaacct ccgggtccta gtcgcccacc
     1381 gcccccagta cgacgactgg tcgctgccca agggcaagtg cgacccaggg gagaccccgg
     1441 aggtcacggc ggtccgcgag gtgctcgaag agaccggttc tcattgccga atagtcgccc
     1501 ccctcggggt cacccgatat cgggtgaccg ggggtgtcaa agaggtcagc tggtttgcca
     1561 tgaagccgct gcccgacagc ccggggtttg ccgagaatcg ggaggtcgac tcgatcaagt
     1621 ggctgtccag acgccgggcg gccgagcttc tcagttacga ccacgaccgg gacctggtcc
     1681 aggagacaga catcaagaag ctctcccaga ccggcatcct ccgcttgctt cgccacagca
     1741 ccgctggtga ccggacgaag tggaagggac aggaccggaa tcggccgctg accaagaagg
     1801 gtcgcagtca ggccgagtcg attgccctct ccctcgctga tgccgggatc gagaggatcg
     1861 tgaccagccc ctacgatcgc tgcctgcaaa cggtggagcc gctgtcccgg gtcaccgggg
     1921 caaggatcga gatctcagac acgctcgccg aggaggcgga gctcgacccg gcatacgccc
     1981 tggtcgacgg tctcgtcggc tccaacgccg tcatttgctc ccatggcgac gtgataccgg
     2041 cgctgatcaa caggatgatg tgggcggggc tgagcctcac ctcgcgcttc tactgctcca
     2101 agggctcgat ctgggaggtc gacgtcgacg gcgggaagtt cacgacggcg cgctacgtgc
     2161 ccccgccgga cagctgacct gccctcagac cgtcttggtg cccgtcgtcc agtcggcgta
     2221 cagaccggcg taggtgccac ccctcgccaa gagctcacga tggtggcccc tctccacgag
     2281 caccccctgg tcaaagacga gcacctcgtc cgacccctcg gcggtcgccg gcctgtgtgc
     2341 gagggtcacc gaggtacgac cctcgatgag acgctctatc gcccgccgca aagcgacatc
     2401 gagcgcagga tcgacggcgg aggtcgcttc gtcgaggacg agcaggtcgg gcgagccgat
     2461 ccatgcccga accagggcga ccagctgccg ctcgcccgcc gagagattcc cgcccctctc
     2521 cccgactctg gtatcgagac cgtcggcgag accctcgacc cagactccca gccccagctc
     2581 gtggaacgcg gtccacactt ctgcgtcact cgcctccgga cgcccgtacc tgacgttgtt
     2641 ggcgatggtg tcgtcgaaga ggaacccctc ctgaggcaca aacgccaccc ggtcacggag
     2701 ctcggcgagg gggagcttcc tggcgttgac tccaccgatc cagacgttgc cctgggtcgg
     2761 gtcgagaagt ctcaccagca gtttggcaaa ggtggtcttg cccgacccgg tctcgccgac
     2821 gacggcgaca cgtgagccga ccgggatgtc gacggtcacc ccgctgagga cctcaccgct
     2881 cgtcgggtag gagaatccaa catcctggaa ccgggcgctt aggccaccca cgtgcagatg
     2941 gtcggggtcc tcgggatcga ccatctccgg ctcggtgtcg agaacaccga gaatccgcct
     3001 cagcccagaa ccggcgctct gcgcgaagtc cagggtctcg acgactatct gaagaggctc
     3061 gatcagcaga ttggtgagga acaagaaggc gaccaatgtc ccggcagtca tcccctggga
     3121 ggttccgatg atgatcccgc cgaccaccac ggcagccgtc agcgcaccag cgaacacctc
     3181 ggcggaggag aacaggatgt tccccagacg tgcggtcctg aactcgatcc agaatcggcg
     3241 ctccagggcg tccgccactc tgctggtggt cgggccctcg atcccatagg cgcggaccac
     3301 cgggatcgcc gatatcgact cgctgaggac cccgagcgag ctgcccacct cggtccggac
     3361 atggtcgtag ttgcgggcca ggacattctg gaaccagcgc atcaacagga gatagaggac
     3421 gacaccgagc accgctacca gtgccaatcg ccactcgtag tagaccatga cccccagtgc
     3481 caggacgacc tggctggtgg cgatgaccat gctgacccca ccccactcca tgaactcctg
     3541 gagcgtggtg acgtccgacg tgacccgcga gaccaggtcg cctcgtcggt ccgcgtcgac
     3601 atggagaacc gagcggcgca acagatggcg aaaggtcagg gtgcgcagat cggatagacc
     3661 ggtcgacgac gacttgatca gccggagcac cgacgctcgg gccaccacca ccccgaccgc
     3721 tagggcgacc a
//
LOCUS       DATITW010000398         1949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_380289, whole genome shotgun sequence.
ACCESSION   DATITW010000398 DATITW010000000
VERSION     DATITW010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380289"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..475
                     /locus_tag="VLS86_05610"
     CDS             209..475
                     /locus_tag="VLS86_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSO50003.1"
                     /translation="MSGEYRGKGEVMEYVRRRRDLAADTFEITVHDVLANDEHGLVIA
                     SGRAKRRGKAVEWRAHGLYRFENGKIAECWLLPEDQTVFDEIWA"
     gene            735..1052
                     /locus_tag="VLS86_05615"
     CDS             735..1052
                     /locus_tag="VLS86_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50004.1"
                     /translation="MLFAVLGVALVIGVVSIMSKSEAPAVVPHLIGERVDPQLDSLRL
                     RLEAADLALGDVSVAPCPEFDIPGVSLERVPGTIIDQHPAGGENVVLSTAVDVTVCLP
                     DRA"
     gene            1211..1762
                     /locus_tag="VLS86_05620"
     CDS             1211..1762
                     /locus_tag="VLS86_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSO50005.1"
                     /translation="MPEIETERLRLRPLGVDDLDPWHRQIFSDPKVTRYLPVREPIPR
                     EDAVERLASQVESWQARGFGVWAVLEKASNQLVGHSGFVTPEATDRIELIYALSRDWW
                     GRGLATEAAAACLRHGFEVLDFKEIGALAFPENEPSIHVMLKLGFVFEDVVPRFGVGL
                     VRYRVDAESFWASSHARLGATGR"
BASE COUNT          345 a          607 c          621 g          376 t
ORIGIN      
        1 ttgtgtgaga cccagtctgg tagagacagg tgacctgcgc gggtgagcga cccatgaagg
       61 tgatgtgacc aggctgggag cggtagctca ttcatcccaa cgagagattg ctgcgacgtg
      121 agtacgatgc acgggctcaa cgggacgacg catcccttgc cgctctcctc accgaggccg
      181 tcgtctggca cgttccgggt cgcgcgcgat ctcgggagag tatcggggca agggcgaagt
      241 gatggagtac gtgcgacgcc gccgagacct tgctgccgac accttcgaaa tcaccgtgca
      301 tgacgttctg gcaaacgatg agcacggact cgtcatcgct tcggggcgag ccaagcgacg
      361 cggcaaggcg gtcgagtggc gtgcccacgg cttgtatcgg ttcgagaacg gcaagatcgc
      421 cgagtgctgg ttgctcccag aggatcaaac tgtcttcgac gaaatctggg cctgagttat
      481 ccgcatctcg gcaagtcccc ttgccttgaa tgtcgtttcg tggccgacgc gacacctcac
      541 gcggactggt gcgtcgaata ggatgatgac ggcgtgaagg aaacctccgc cggacggcgg
      601 tgtcgttcca gcgggcctga ccgggaaggc gaatggcaaa ctcggattct caggacgtgg
      661 tcgagagcag caaccctccc tcgaacaccg cacgcacagt gccaccgacc ttatcgtcct
      721 tcttccgtcc caggatgctg ttcgcggtgc tcggcgtggc tctggtcatc ggtgtcgtat
      781 cgatcatgtc gaaatctgag gctccggcag tcgttcctca tctcatcggt gaacgtgtcg
      841 acccgcagct cgacagcttg cggttacggc ttgaggccgc tgacctcgct ctcggcgacg
      901 tctccgttgc cccctgtcct gagttcgaca tcccgggcgt ctccctcgaa agagtccccg
      961 gcacgatcat cgatcagcac ccggccgggg gcgagaacgt cgtcttgtcc acagcggtag
     1021 acgtgactgt gtgcctcccc gacagggcct gactcaaagc gcctccgggg agcagccggt
     1081 ccacctcgaa cccgtacatt ctgtcgcggg agttggcagt cgcttccgcc ggaggcgaca
     1141 tctaggcccg cctggaaggt gggttgcctc ggtaggtcga gggacgcaac cgcaccgcca
     1201 tactcaccac gtgcccgaga tcgagacaga gcgactgcgg ctcaggccac tgggcgtcga
     1261 cgatctggat ccatggcatc gtcagatctt cagcgatccc aaggtcacgc gatatctgcc
     1321 cgtccgagag ccgatcccga gggaggatgc ggtcgaacga ctggcatctc aggttgagag
     1381 ttggcaggcc cgtggcttcg gcgtgtgggc cgttctcgag aaggcgtcga accagctcgt
     1441 gggccactcc gggtttgtca cacccgaagc gacagaccgc atcgagctga tctacgcgct
     1501 gagtcgagac tggtggggaa ggggactcgc gacagaggcc gccgccgcct gtctgcgcca
     1561 tggcttcgag gtccttgatt tcaaggagat cggggcgctt gcgttcccgg agaacgagcc
     1621 gtcgatccac gtcatgctca agcttggctt tgtcttcgag gacgtcgttc ctcggtttgg
     1681 agtcgggctc gtccggtaca gggtcgatgc ggaatccttc tgggcttcct cacacgcacg
     1741 tctgggagcg acaggcagat agttctcgtc ctctatcgcg actgttggtg gcggaggctc
     1801 cggggctcag tctgttccga tgggtcatcc ttcgcctcca ccacaacccg gctctggggc
     1861 gatggaaact cgagccaagt cggccgaccc gagtccgagc aggcaatccc cgtcaagcgc
     1921 caagcgacac ccctcctgcc tgcctcccc
//
LOCUS       DATITW010000399         1891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_340222, whole genome shotgun sequence.
ACCESSION   DATITW010000399 DATITW010000000
VERSION     DATITW010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340222"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /gene="murC"
                     /locus_tag="VLS86_05625"
     CDS             <1..1143
                     /gene="murC"
                     /locus_tag="VLS86_05625"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HSO50006.1"
                     /translation="AVPDRDPELVAAGAAGIPIWRRPQLLEALTGLLPTVGATGTHGK
                     TTTTALLISGLRSAGVDPSFIVGGDLADLGTNGHYGEDPLLVLEADEAFRTFERLSLV
                     GLVVTNVDSEHVEHFGSPDELMETFIAVASSVNGPVVACLDDPGSAQVAARVGAVTYG
                     LDPAATWRIGDFTSNQGGSRFTLTGPDDGVDVKLRRPGLHNARNAAGALGLLATLGHD
                     LAKCAQGMAAFRGVGRRWELRGVVEGITLIDDYAHHPTEVAATLSAAVGVVPGRVWAV
                     FQPHLYSRTERLQDEFGEALSTADVVVVTDVYGSREEPVPGVTGELVAEAVRRRGREV
                     HYVAHKTELARFLADRVAAGDLVLTMGAGEITLLPTELTAILTGSE"
     gene            1140..>1891
                     /locus_tag="VLS86_05630"
     CDS             1140..>1891
                     /locus_tag="VLS86_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSO50007.1"
                     /translation="MSMLDDLIASGVVTRDVPLGPLTTYKSGGVARYLVVCEDLTTLV
                     GLSATGELAEIPVLVLGRGSNLVVADRGFPGLVIRLGSGFTVVDIDGSTVTAGSAVSL
                     PVLARASVNAGRSGLEFFVGVPGSVGGAIRQNAGCFGVETRDRLIRATVLNLADGTVT
                     TRAPDDLDLSYRHSNLGPTEVVTEAVFQTDEGDVEAGLLELRRITRWRRDNQPGGTFN
                     AGSVFKNPTGTTAGELIDSVGLKGLKVGDVSVS"
BASE COUNT          281 a          594 c          668 g          348 t
ORIGIN      
        1 gctgtccctg accgcgaccc tgagttggtg gcggctggag ctgcggggat tccgatctgg
       61 cgacgtcccc aactgctcga ggcgctcaca gggttgttgc ccactgtcgg ggccaccggt
      121 acccacggca agacgacgac caccgccctt ctcatcagcg gcctccgctc ggccggggtc
      181 gatccctcgt tcatcgtggg cggcgatctc gccgatctgg gcaccaacgg ccactacggc
      241 gaggacccgt tgttggtgct cgaggccgat gaggccttcc gcaccttcga acgcctcagc
      301 ttggtgggct tggtggtgac caatgtcgat tccgagcacg tcgagcattt cggttcgccc
      361 gacgagctga tggagacctt tatcgcggtc gcatcgtcgg tgaacggccc ggttgtcgcc
      421 tgtctcgacg atcccggctc ggctcaggtg gcagccaggg tgggtgcggt gacctacggg
      481 ctcgacccag ccgccacctg gcggattggc gatttcacgt cgaatcaggg cgggagccga
      541 ttcaccctca ccgggcccga cgacggggtt gacgtgaagc tgcgacgacc agggctgcac
      601 aacgcccgaa acgctgccgg cgccctcggt ctcttggcga cgctcggcca cgatctggcc
      661 aagtgcgccc aggggatggc tgcgtttcgc ggcgtcggcc ggcgttggga gcttcgcggc
      721 gtggtggaag gcatcaccct gatcgatgac tacgcgcatc acccgaccga ggtcgctgcc
      781 actctcagcg ctgcggtcgg agtggtaccc ggaagggtgt gggcggtgtt tcagccccat
      841 ctctacagcc gcaccgaacg gcttcaggat gagttcggcg aagccctgag caccgccgat
      901 gtggtggtgg tgaccgacgt ctacggctct cgcgaggagc cggtgcccgg agtcaccggg
      961 gagctcgtgg cggaggcggt gcggcgtagg ggacgtgaag tccactacgt ggcgcacaag
     1021 accgaactcg cccgctttct cgcggaccgg gtggctgccg gcgatctcgt gctgaccatg
     1081 ggggccgggg agatcacgct gctcccgacc gagttgaccg caatcctcac cgggtcggaa
     1141 tgagcatgct cgacgacttg atcgccagcg gcgtcgtcac ccgcgatgtc ccgctcgggc
     1201 ccctgaccac ctacaagtcg ggaggagtcg cccgttatct ggtggtctgc gaggacctca
     1261 ccaccctggt gggcctctct gcgaccgggg agttggctga gatcccggtg ttggtgttgg
     1321 gccggggatc caacctggtg gtggccgacc ggggcttccc cgggctggtg attcggctgg
     1381 gatctgggtt cacggtcgtc gacatcgacg gctccaccgt gacggcgggg tcggcggttt
     1441 cgttgccagt tctcgccaga gccagtgtca atgccgggcg cagcgggttg gagttcttcg
     1501 tgggtgtgcc cggctcggtg ggcggggcga tccgtcagaa tgccggctgt ttcggggtcg
     1561 agaccaggga tcgtctgatc agggccacgg tcctgaacct tgccgacggc accgtgacta
     1621 cccgtgcccc ggacgatctg gatctctcct accggcactc caacctgggc ccgaccgagg
     1681 tggtcaccga ggcggtattc caaaccgatg aaggggacgt tgaagccggt ctgctggaac
     1741 tgcggcggat cacccgttgg cgacgggaca atcagccggg cgggactttc aatgccggga
     1801 gcgtcttcaa gaacccgacc gggaccactg cgggggagtt gatcgactcg gtcgggctca
     1861 agggtctcaa agtgggcgat gtctcggtgt c
//
LOCUS       DATITW010000400         1771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_140017, whole genome shotgun sequence.
ACCESSION   DATITW010000400 DATITW010000000
VERSION     DATITW010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140017"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VLS86_05635"
     CDS             complement(<1..499)
                     /locus_tag="VLS86_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019979094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50008.1"
                     /translation="MTATDRLRELRGSWSAPLAPGDPAELPGLLTQAVDSLVIEVAAG
                     LDMSGWVVVATGGYGRSEMCLQSDIDIMILGRPSQETVRQLFYPLWDTGMKVGHSVRS
                     VKEAAAGAAENLETLCSLLSARLILGDPKQLDQLERQISVLLRRQRSLLDLLAEEERR
                     VREREP"
     gene            complement(496..834)
                     /locus_tag="VLS86_05640"
     CDS             complement(496..834)
                     /locus_tag="VLS86_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSO50009.1"
                     /translation="MKLIIAYIKPFKLDEVKEKLRSIGVTGMSVGEVRGFGRQAGQTE
                     VYRGAEYQVDFVPKNRIEVAVDDDAASRVVEAIEAAARTGEIGDGKILVLGVEEVVRI
                     RTGERGADAL"
     gene            complement(845..>1771)
                     /locus_tag="VLS86_05645"
     CDS             complement(845..>1771)
                     /locus_tag="VLS86_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSO50010.1"
                     /translation="FQMMFAIITPALISGAVAERMKFSAWVLFITLWSILVYPVVAHW
                     GFAADGLLFEWGARDFAGGLVVHVNAGIAALALVFALGPRKGFKEESMRPHSLPLTLL
                     GTGILWFGWFGFNAGSALAADANAITAFTTTQIAASVAALGWVVAEWWKTGKPTTLGA
                     ASGAVAGLVAITPCAGFVSPLASIAIGLIAGVVCFWAVSLKFRFNYDDSLDVVGIHLA
                     GGIVGSLLLGIFASTAVNEAVGLDGLLFGETSLFLKQVGALGAVLVYGFVASYLLARL
                     TKAITGLRADEKAEIDGLDIALHEERAYVLTV"
BASE COUNT          350 a          611 c          540 g          270 t
ORIGIN      
        1 agggctcccg ctcacggacc cgccgttctt cctcggccag tagatcgagc agcgatcgct
       61 gacgacgcaa aaggaccgag atctgccgct cgagctgatc gagttgcttg gggtcaccga
      121 ggatcaggcg ggcgctgagc agcgagcaca gggtctcgag gttctcagcg gccccggccg
      181 ctgcttcctt gaccgagcga accgagtgcc cgaccttcat cccggtatcc caaagcggat
      241 agaacagctg gcgcaccgtc tcctgggagg gacgacccag gatcatgatg tcgatgtcgg
      301 actgcaggca catctcggag cgcccatagc cgccggtggc gaccaccacc catcccgaca
      361 tgtcgagacc ggccgcgacc tcgatcacca gcgagtcgac cgcctgggtg agcagcccgg
      421 gcaattccgc cggatcgccg ggagccagag gggcggacca tgagcccctc agctcccgaa
      481 ggcgatccgt agccgtcaca aagcgtcagc tccccgctca ccggtccgga tgcggacgac
      541 ctcctcgacc ccgaggacga ggatcttgcc gtcaccgatc tccccggttc gggctgcggc
      601 ttcgattgcc tccaccaccc gggaagcggc gtcgtcgtcg acagccacct ctatccggtt
      661 cttcggaacg aagtcgacct ggtactcggc acctcggtag acctcggtct gcccggcctg
      721 gcggccgaag ccacgcacct caccaaccga catcccggtc acaccaatcg atctgagctt
      781 ctccttgacc tcgtcgagtt tgaagggctt gatataggcg atgatcagct tcattgttat
      841 ttcctcagac cgttagaacg taggcgcgtt cttcatgcag agcgatgtcg agcccgtcga
      901 tctccgcctt ctcgtccgcg cggagaccgg ttatcgcctt ggtcaggcgg gccaggagat
      961 aggacgccac gaatccgtac accaacacgg cgcccagcgc tcccacctgc ttgaggaaga
     1021 ggctggtctc accgaagagg agcccatcca atcccaccgc ttcgttcacc gcggtggagg
     1081 cgaatatccc caacaggagc gagccaacga tcccacccgc caggtggatc ccgaccacgt
     1141 cgagcgagtc gtcgtagttg aaacggaact tcaggctgac cgcccagaaa cagacgaccc
     1201 cggcaataag gccgatcgca atcgacgcca gggggctgac gaagccggcg cagggtgtga
     1261 tggcgacgag gccggccacc gcccccgagg ccgctcccag ggtcgtcggc ttcccggtct
     1321 tccaccattc ggcgaccacc caccccaggg ccgccaccga ggcggcgatc tgagttgtgg
     1381 tgaaggcagt gatggcgttg gcgtcggcgg cgagcgcgga gccagcgttg aacccgaacc
     1441 agccgaacca caggatcccg gtccccagca gggtcaaagg cagggaatgg gggcgcatcg
     1501 actcctcttt gaaccccttt cgaggcccga gggcgaacac gagggcgaga gcggcgatac
     1561 ctgcgttgac atgcaccacc aggcctccgg cgaagtccct ggctccccac tcgaagagca
     1621 gcccgtcggc ggcgaaaccc cagtgcgcca ccaccggata caccaggatc gaccacaggg
     1681 tgatgaacag gacccaggcg gagaacttca tccgctccgc gaccgcccct gagatcagcg
     1741 ccggggtgat gatggcgaac atcatctgga a
//
LOCUS       DATITW010000401         1517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_179933, whole genome shotgun sequence.
ACCESSION   DATITW010000401 DATITW010000000
VERSION     DATITW010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179933"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VLS86_05650"
     CDS             <1..547
                     /locus_tag="VLS86_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HSO50011.1"
                     /translation="PGQARADWEILNDLAERAGRPIGLASAESVAKEISEVAPAYVGV
                     TWDLLDWEARDGAVVPMSGSQSLRHIPVALKGDKAPSAPLILHVARTLYDDGVMMRQS
                     PFLHQLAPGAVAHLNPEDAKKAGAQQGHPVKVVTKHGEGEFAAVLDEGTPAGVVFVPF
                     NQPGANSLGTEAVVRVTAVGK"
     gene            544..>1517
                     /locus_tag="VLS86_05655"
     CDS             544..>1517
                     /locus_tag="VLS86_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HSO50012.1"
                     /translation="MTWPEFWIVVLRVILTFGLLLVATLINVWAERKILADMQNRIGP
                     KRAGPWGILQTVADGLKLFFKEAITPRKVELGMYLLAPFLAVVPAFLIFMMVPWGKPF
                     TIAGNEVALQGADLNIGLLFILAMSSLAVYSVVFAGWASGSKYPLLGGVRATAQAISY
                     EAAMGLAMVAVVMFTATQGDGGTLSLAEIVERQSGTWGDIIPAFEPVLQFVPRWNVFP
                     QIVAFIIFFIAIVAETNRAPFDLVEAEQEIVGFHTEYSGMRFAMFFLAEYVNIFSMCA
                     IAASLFLGGWNGPTFEGSVPAIISGILPTVWFLAKTYFLIFLFFWIRAT"
BASE COUNT          246 a          488 c          480 g          303 t
ORIGIN      
        1 accgggacag gcccgcgccg actgggagat actcaacgat ctggccgagc gggccggtcg
       61 ccccatcggt ttggcgtcgg cagagtccgt cgcgaaagag atctctgagg tagcccccgc
      121 ctacgtcggc gtcacctggg atctgctcga ttgggaggcg cgtgacggcg cggtcgtgcc
      181 gatgtcgggc tcgcagtcac tgcgccacat cccggttgcg ctgaaggggg acaaggcgcc
      241 atccgccccg ctgatcctgc acgtggccag aaccctgtac gacgatgggg tgatgatgag
      301 gcagagcccg ttcctgcacc agctcgcccc aggcgcggtg gctcatctga atccggagga
      361 cgcaaagaag gcaggtgccc aacagggaca cccggtcaag gtcgtgacca aacacggtga
      421 gggagagttc gccgcagtcc tcgacgaggg gacgcctgcc ggagtggtat tcgtgccttt
      481 caaccaaccc ggtgcgaact cattgggcac cgaggccgtg gtccgggtga ccgcggtggg
      541 caaatgacct ggcccgagtt ctggatcgtc gtcctccggg tgatcctcac cttcggtctc
      601 ttgttggtgg ccacattgat caacgtgtgg gcagagcgaa agatcctcgc cgacatgcag
      661 aaccggatcg gccccaagcg ggccggcccc tggggcatcc tgcagacggt cgcagacggc
      721 ctgaagctgt tcttcaaaga ggcgatcact ccacgcaagg tggagttggg gatgtacctg
      781 ttggcgccgt tcctggccgt ggtccccgcc ttcctcatct tcatgatggt cccctggggc
      841 aagccgttca ccatcgccgg aaacgaggtg gccttgcagg gcgccgacct gaacatcggg
      901 ctgctcttca tcctcgccat gtcttcgctg gcggtctatt cggtggtgtt tgcgggatgg
      961 gcttcgggct ccaagtaccc gctcctcggc ggggtgcgcg ctacggccca ggcgatctcc
     1021 tacgaggcgg ccatgggcct ggccatggtt gccgtggtca tgttcaccgc cacccaggga
     1081 gacgggggca ctctgtcgtt ggccgagatc gtcgaacgtc agtccggcac ctggggcgac
     1141 atcattccgg ccttcgagcc ggtgctgcag ttcgtccccc gctggaacgt gttcccacag
     1201 atcgtggcct tcatcatctt cttcatcgcg atcgtcgccg agaccaatcg cgccccattc
     1261 gacctcgtcg aggcagagca ggagatcgtc ggcttccaca ccgaatattc ggggatgaga
     1321 ttcgccatgt tcttcctcgc cgaatacgtg aacatcttct cgatgtgcgc catcgccgcc
     1381 tccttgttcc tgggcggctg gaacgggccc acgttcgagg gatcggtccc cgcgatcatc
     1441 tccggcatcc tccccaccgt ctggtttctg gccaagacct actttctgat cttcctgttc
     1501 ttctggatca gggcaac
//
LOCUS       DATITW010000402         1631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_740985, whole genome shotgun sequence.
ACCESSION   DATITW010000402 DATITW010000000
VERSION     DATITW010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740985"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /gene="gatA"
                     /locus_tag="VLS86_05660"
     CDS             <1..1396
                     /gene="gatA"
                     /locus_tag="VLS86_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017537188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HSO50013.1"
                     /translation="LEGVLERAASYEAQVHAYLPLDRERALETAARADADLADGNDRG
                     PLHGIPIALKDNMCTRGVETTAASQILAGYRPPYDATVVSRLADAGAVVVGKTNLDEF
                     AMGSSTENSAYGPTYNPWDTTRVAGGSSGGSAAAVAIGSALAGLGSDTGGSIRQPAAL
                     CGVVGMKPTYGTVSRYGLIAFASSLDQIGPLTRTVEDSALTLEAIWGHDPFDGTSYAG
                     VYPDPKAGLGRGVSGLRVGVVKELSGEGYEPAVESATADMVANLEGAGVEVIEVSLPS
                     FDIALSAYYLIAPAECSANLARFDGIRYGHRGEGATTEALMARSRAEGFGPEVTRRIL
                     LGTYALSAGYYDAFYGQAQRVRAAIQEDFARAYQQVDVLVSPTSPTVAFEAGARTSDP
                     LAMYLSDICTIPSNLAGHPAISLPVGLDGQGLPIGFQVMAPPLGEEVMYQVASEVERL
                     AGFEVAPGLQEVAS"
     gene            1393..>1631
                     /locus_tag="VLS86_05665"
     CDS             1393..>1631
                     /locus_tag="VLS86_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HSO50014.1"
                     /translation="MSWEPVIGIEVHVELLTDSKMFCGCAVGFGAEPNTNVCPTCLGL
                     PGALPMINRAAIESIMAIGLALNCEVSERSVFHRK"
BASE COUNT          245 a          544 c          553 g          289 t
ORIGIN      
        1 gctcgaaggc gtcctggagc gggctgccag ctatgaggcc caggtccacg cctatctccc
       61 cctcgaccgg gagcgggcgc tggagaccgc ggctcgggcc gacgccgacc tggcggacgg
      121 gaacgaccgg gggccgctgc acggcatacc gatcgcactc aaggacaaca tgtgcacccg
      181 gggggtggaa acgaccgccg cctctcagat cctcgccggg tatcgccccc catacgacgc
      241 gaccgtcgtt tcccgactcg ctgacgccgg tgccgtggtg gtgggcaaga cgaatctcga
      301 cgagttcgcg atgggctcct cgaccgagaa ctcggcctac ggccctacct acaacccgtg
      361 ggacacgacc cgggtagccg gtggctcctc gggcggttcg gcggccgcgg tcgccatcgg
      421 ttcggcactg gcaggtctgg ggtccgacac cggcggctcg atccgtcaac cggccgccct
      481 ttgcggcgtg gtggggatga aacccaccta tgggaccgtg agccgttacg ggctgatcgc
      541 tttcgcttcc tcgctcgatc agatcgggcc tctcacgcga acggtcgagg actcggcgtt
      601 gaccctcgaa gccatctggg gtcacgaccc cttcgacggc acctcgtacg ccggtgtcta
      661 tcccgacccc aaggccgggc tgggccgtgg agtgagtggg cttcgggtcg gtgtggtgaa
      721 ggagctctcc ggcgagggat acgagccggc tgtcgagtcg gcgacggcgg acatggtcgc
      781 caacctcgag ggtgccgggg tcgaggtcat cgaggtgtcg ttgccgtcat tcgatatcgc
      841 cctctctgcc tattacctga tcgcccccgc cgagtgctcg gcgaacctgg cccgcttcga
      901 cgggattcgc tacgggcacc gcggcgaggg ggcgaccacc gaggcactca tggcgcggag
      961 cagggccgaa ggtttcgggc ctgaagtcac ccggcggatc ctgctcggca cctatgcgtt
     1021 gtcggccggg tactacgacg ccttctatgg acaggcgcag agggtccggg ccgcaatcca
     1081 ggaggacttc gcacgggcct atcagcaggt cgacgtgctg gtctcgccca cgagcccaac
     1141 ggtggccttc gaagcggggg cgcgcacctc cgaccctctc gccatgtatc tctccgacat
     1201 ctgcaccatc ccctccaacc tggccggcca tcctgcgatc tccctcccgg tgggtctcga
     1261 tggccagggc ctcccgatcg gattccaggt gatggcgccc ccacttgggg aagaggtcat
     1321 gtatcaggtt gcgtccgagg tggagcgact cgccggtttc gaggtggctc cgggcctcca
     1381 ggaggtcgcg tcgtgagctg ggagcccgtg atcgggatcg aggtccacgt cgagcttctc
     1441 accgattcca agatgttctg tggttgtgcg gtgggattcg gcgccgagcc caataccaac
     1501 gtctgcccga cctgtctcgg gcttcccggc gcgcttccga tgatcaaccg ggccgcgatc
     1561 gagtcgatca tggccatcgg gcttgccctc aactgtgagg tttcggagcg gtcggtgttc
     1621 caccggaaga a
//
LOCUS       DATITW010000403         4397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_420780, whole genome shotgun sequence.
ACCESSION   DATITW010000403 DATITW010000000
VERSION     DATITW010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420780"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VLS86_05670"
     CDS             <1..933
                     /locus_tag="VLS86_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HSO50015.1"
                     /translation="RYSYSWIKGAVGPAAERQVNKFLDSLLNDLEEAGVKDQPLGVDF
                     IDINLVRQFEERGINWTDGMTAMMEARAIKSPDEIKAFSIVGAICDYVHYEITNFIKP
                     GLTENEVAAFGFEKLYSIPGMEDVEDVIVSSGPNAWPNWRNFSDRMIRPGELVIVDLA
                     ALTWNGFKSCVYRTYCVGGKPTDEHQKTYDLAHKWLWDSIEATKPGVTTADVASKWPS
                     AKEVWGYEEEDQAAANFWGHGLGLAQYDPPVISRIWSLDFPVEIQPGMTFALETQHGK
                     LHDHGVRLEEMLYVTDTGIEMVSSYASHEIISTT"
     gene            939..2570
                     /locus_tag="VLS86_05675"
     CDS             939..2570
                     /locus_tag="VLS86_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HSO50016.1"
                     /translation="MILDLDHEASLDPGRVGAKAAWLAMARRVGLPVLPGLVVERPAS
                     TRHMALGAETLSSRGSGGARLSVSGEPIDIADDLVARAARLGDHLVARSSTALEGSGE
                     WSGAFTSYLDLAPSDLPKAVAGCWASAFSVDALERQRSASIDPGSFTMAVLIQPALDP
                     TAGGTARLEESGTVVVHGVKGSPAPLLQGWSGGHEARASDAGWVGDDLIELVGLPALD
                     EIAAQLRKANESLGVNHCEWALDGTIWFLQLDDHKPPFQSSGSDRHHVEADRGLVPVV
                     KVVAQAPGRLGEELILPWALGGLPQTEPVTVVPEPDLPLTASLRDELVSQVWDMPVGP
                     ALAAARECMVELRGPDPHRALGRIQDLLPPHPETAARLLALVGAVRAAMVGGGVVRDT
                     AAAWHLSLPQVAAVLAGATAGPVSRFGVGRWEPFLASVTLAVGDRRQGTPASPGIGAG
                     LRSHVTNPESGHFPTRGIISSPQPIPNLAPLLWDAAGLVTATGSPAAHLFESARALGV
                     PAVCGLDLDEADEIVAVDGHSGVVATLSLNGDDDV"
     gene            2563..3438
                     /locus_tag="VLS86_05680"
     CDS             2563..3438
                     /locus_tag="VLS86_05680"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSO50017.1"
                     /translation="MSERIGVIGLGVMGSAMAANLLAAGHDVAGFDIDPDKTAGFTQR
                     GGRAGRSAGEVASDSDLVVLSLPTVTALAGVAAEIAGSAREDLVCLEAGTFPLEAKMA
                     AYDTLSAAGVDLLDTPLSGTGLQAADATLVVFASGSRAGFDRARPVFEAIGRSTHYLG
                     DFGNGSRMKYIANLLVAVHGLAAAEAHTLGLAAGLDPALVQEVMEDGVGSSKIFEIRG
                     PMMVANDYPPAARLDILLKDATIIRDFARSVGAVTPLLDAAVPVYFESSEAGLGGLDA
                     AALCRYLETQSGIER"
     gene            3435..>4397
                     /locus_tag="VLS86_05685"
     CDS             3435..>4397
                     /locus_tag="VLS86_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSO50018.1"
                     /translation="MIGRPLRRLEDPRLITGQGRYVDDMVMDGLVHAVFVRSIEAHAS
                     IRGVSVDPDAIGDARVFTAADLGLTKPMPNQNPSPVITQSIQAPPLAVDEVCYVGQPF
                     AVVVAASVAAAIDGAEAVEVDYEPLPVVIDHRRALDPEAPAAHLGSGSNLVATLTASF
                     GEVDAAFAAAAHIVSVDVDQHRGALASMEGRAVLGRWDDNERRLDLWSSTQSPHAVRA
                     NLAPYLGLSPDELRVIAPDVGGGFGPKAAIYSEEYVIAALALHLRRPVKWNELRREHF
                     TSTSQQRGQSGRMEAAIDDQGRILGLRARLVHDSGAYVPYGVVVP"
BASE COUNT          684 a         1469 c         1507 g          737 t
ORIGIN      
        1 cgctactcct actcgtggat caagggggcg gtggggccgg ccgccgagag gcaggtcaac
       61 aagtttctcg attccctgct caacgatctc gaagaggccg gggtgaagga ccaaccgctg
      121 ggcgtcgatt tcatcgacat caacctggtt cgccagttcg aagagcgggg aatcaactgg
      181 accgacggga tgaccgcgat gatggaggcc cgggccatca agagccccga cgagatcaag
      241 gccttctcga tcgtcggcgc catctgcgac tacgtccact acgagatcac caacttcatc
      301 aagccagggc tcaccgagaa cgaggtggcg gcgttcggct tcgagaagct gtactccatc
      361 ccgggcatgg aggacgtgga ggacgtcatc gtgtcctccg ggcccaacgc ctggcccaac
      421 tggcggaact tctccgatcg gatgatcaga cccggcgagc tggtgatcgt cgacctggca
      481 gccctcacct ggaacgggtt caagtcctgc gtctatcgaa cgtactgcgt cgggggcaag
      541 cccaccgacg agcaccagaa gacctatgac ctcgcccaca aatggctgtg ggactcgatc
      601 gaagccacca agccgggagt gacgaccgcc gacgtggcgt cgaagtggcc ctcggccaag
      661 gaggtttggg gatacgagga ggaggaccag gctgcggcca acttctgggg ccatggcctc
      721 ggtctcgccc agtacgaccc gcccgtcatc tcccgcatct ggtcgctcga cttcccggtc
      781 gagatccagc cgggcatgac cttcgccctc gagacccagc acggcaagct ccacgatcac
      841 ggtgttcgtc tcgaggagat gctctacgtg accgataccg gcatcgagat ggtctcgagc
      901 tacgcatccc acgagatcat ctcgaccacc tgaccggggt gatcctcgac ctcgaccacg
      961 aggccagcct cgatccgggt cgggtcggcg ccaaggctgc ctggctggcg atggcgagac
     1021 gggtcgggct gcccgttctt cccggcttgg tggtggagag gcccgcctcg acccgtcaca
     1081 tggccctggg cgcggagacg ctgtcgtcgc gcggctcggg cggcgcacgt ctctcggtct
     1141 cgggagagcc gatcgacatc gccgacgacc tggtggccag ggcggcccgg ctcggagatc
     1201 atctcgtcgc tcgctcctcg actgccctcg aaggctccgg ggagtggtcc ggcgcattca
     1261 cctcctacct cgacctggcg ccttccgacc tccccaaggc ggtcgccggg tgctgggcct
     1321 cggccttctc ggtggacgcg ctggagcgtc agcgatcggc atcgatcgat cccggctcgt
     1381 tcacgatggc agtgctcatc cagccggcat tggatccgac cgcaggtggc acggcccggc
     1441 tcgaagagag cgggaccgtc gtggtccacg gggtgaaagg gtctcccgct cccctgctcc
     1501 agggatggag cggaggtcat gaggcgcggg cctccgacgc gggttgggtc ggtgacgacc
     1561 tcatcgagct ggttggcctt ccggccctgg atgagatcgc cgctcaactc aggaaagcca
     1621 acgagtcgct cggggtcaac cactgcgaat gggcgctgga cgggacgatc tggtttctcc
     1681 agctcgacga ccacaaacct ccgttccaga gctcgggctc ggaccgacat cacgtcgaag
     1741 ccgatcgcgg ccttgtgccc gtggtgaagg tcgtggccca ggctccgggg cgtcttggcg
     1801 aggagctgat cctcccctgg gccctggggg gactgcccca gacggagccg gtaactgtcg
     1861 ttcctgagcc cgatctgccg ctgacggcca gcctccgcga cgagctcgtt tcccaggtat
     1921 gggacatgcc ggtgggacct gccctggctg cggccagaga atgcatggtc gaactgcggg
     1981 ggcccgatcc gcaccgggca ctgggccgga tccaggatct gctcccgccc cacccggaga
     2041 cggcggcgcg gctcctggca ctggtcgggg ccgtccgggc cgccatggtg gggggaggcg
     2101 tggtgcggga caccgcggcc gcctggcatc tcagccttcc ccaggtcgcg gcagtgctcg
     2161 ccggcgcgac ggctggccca gtatcccgat tcggtgttgg tcgctgggag cctttcctgg
     2221 cctcggtcac gctggcggtt ggcgaccgac ggcagggcac accggcgtct ccagggatcg
     2281 gcgccgggct tcggtcccat gtcaccaacc ccgagtcagg ccatttcccg acgcggggga
     2341 tcatcagctc cccccagccg attcccaacc tggcacccct cctgtgggat gctgccggcc
     2401 tggtcactgc caccggaagc ccggctgccc acctgttcga atcggcgcgg gcactgggcg
     2461 tgccggctgt gtgcggattg gacttggatg aagccgacga gatcgtggct gtggacgggc
     2521 actccggggt ggtcgccacc ctgtcgctga atggagatga cgatgtctga gagaatcggt
     2581 gtgatcgggc tgggcgtgat gggctcggcg atggcagcca acctcctcgc cgcgggtcat
     2641 gacgtggccg gcttcgacat cgacccggac aagacggccg gcttcaccca gcgtggtggc
     2701 cgagccggtc gatcggcagg cgaggtcgca tccgactccg acctggtcgt gctctccctg
     2761 cccaccgtca ccgccctggc cggggtggcc gccgagatcg caggcagcgc ccgcgaggat
     2821 ctcgtctgct tggaggccgg gacattcccg ctcgaggcga agatggctgc ctacgacacc
     2881 ctcagcgcgg ccggtgtcga cctgctcgac accccgctgt ccggcaccgg tctccaggcc
     2941 gccgacgcga ctctggtggt cttcgcctcg gggagccgag ccggattcga tcgggcccga
     3001 cccgtgttcg aggcaatcgg ccgctccacc cactacctcg gcgacttcgg gaatgggtcg
     3061 aggatgaagt acatcgccaa cctgctggtg gcggttcacg ggctggcggc ggccgaggcc
     3121 cacacactgg gcttggcagc cggtctcgat cccgcgctgg tccaggaggt catggaggac
     3181 ggagtcggtt catcgaagat cttcgagatc cgaggcccga tgatggttgc caacgactac
     3241 ccgccggcgg cgcggctcga catcctgctc aaggacgcca cgatcatccg cgacttcgcc
     3301 agatcggtag gagcggtcac cccgttgctc gatgccgcgg tcccggtgta cttcgagtcc
     3361 agcgaggcgg gtctcggcgg gctcgacgct gccgccctgt gccggtatct ggagactcag
     3421 agcgggatcg agcggtgatc ggccgacctc tgcgccgtct ggaggacccg cggctgatca
     3481 ccggccaggg ccgttacgtc gacgacatgg tgatggacgg gctggtccac gctgtcttcg
     3541 tgcgcagcat cgaggctcat gcctcgatcc ggggagtgtc ggtcgatccc gatgcgatcg
     3601 gagacgcccg ggtgttcacc gcagccgatc tcggattgac caagccgatg cccaatcaga
     3661 acccgtcgcc ggtcatcacc cagtcgatcc aggctccccc actggcggtc gacgaggttt
     3721 gctacgtggg ccagccgttt gcggtggtgg tcgctgcctc ggttgcggcg gcgatcgatg
     3781 gggccgaggc ggtcgaagtc gactacgagc cgctgccggt ggtcatcgac caccgcaggg
     3841 ccctcgatcc ggaggcgcca gcagctcatc tgggctcggg ctcgaacctg gtggcgaccc
     3901 tgaccgcgag ctttggggaa gtggacgcgg ctttcgccgc ggcggcccac atcgtcagcg
     3961 tcgacgtcga ccagcaccgg ggcgcgctcg cctcgatgga agggcgggcg gtgctcggcc
     4021 gttgggacga caacgagcgg agactcgacc tgtggagctc gacacagtcg ccacatgcgg
     4081 tgcgtgccaa cctcgcgccc tacctcggac tctcaccgga cgagttgcgg gtgatcgccc
     4141 ccgatgtcgg cggcgggttc gggcccaagg cggccatcta ctcggaggag tatgtgatcg
     4201 ccgccctcgc cctccacttg cgccggccgg tgaagtggaa cgagttgaga cgggaacatt
     4261 tcacctccac cagccagcag cgcggccaga gtggccggat ggaggcggcc attgacgacc
     4321 aagggcgaat tctcggtctg agggcccgtt tggtccacga ctccggcgcc tacgtgccct
     4381 acggcgtggt ggtcccg
//
LOCUS       DATITW010000404         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_780993, whole genome shotgun sequence.
ACCESSION   DATITW010000404 DATITW010000000
VERSION     DATITW010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780993"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VLS86_05690"
     CDS             <1..520
                     /locus_tag="VLS86_05690"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HSO50019.1"
                     /translation="GPESKVLLTTESKGATPRLHDIAVMSRIGLDLSPIDPGDQESRL
                     WLDALIWPEHVERRARLRAALEVMAAVEVEMVAGDALVTLPETLAGLPGADPAIVVNS
                     FTLIQFSPEQRDQLEEIAAAARAQRPVFRASMEALDKNDDWARLVIDDGSGPRQVGEA
                     HPHGEWIDLSER"
     gene            complement(517..1221)
                     /locus_tag="VLS86_05695"
     CDS             complement(517..1221)
                     /locus_tag="VLS86_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSO50020.1"
                     /translation="MEKTLVITGGTGGLGSALVRRLVFEDYRLAVTYLMPEEAAQFEE
                     EFDIDEDRLLLVRVDATNAEAVNSLMKNVADRWGSIHGLCSLVGGWAGGRDVAETDDV
                     RFERMIDLNLRSAFYAVRAVVPYLVETGWGRIVTVGSRAAVDAPTGQGAFNIAKSGVV
                     ALTKSVAQELDGTGVTANVLMPSVIDTPATRQSLPYADYVTWPTPDEIAAVAHFLLSD
                     ESGVINGAEIPVYGRA"
     gene            complement(1247..>2223)
                     /locus_tag="VLS86_05700"
     CDS             complement(1247..>2223)
                     /locus_tag="VLS86_05700"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HSO50021.1"
                     /translation="PTSDRLQFLVGDHPVPGAASLHAAETVLEAVQDASPAIPIIALI
                     SGGGSSLCEKPRDGVPGEFLAEVTRRLIAGGAGIEEMNLVRAHLSAIKGGGVSRAAGR
                     PIDTYVISDVAGAHPGVVASGPTIPRAADAEMATLIMRRAAIDVPDEVLAVLRTPQPA
                     IAAPEVTLLANGMDAARGVASAVSGPVALRSDWLAGPVEPCLERFFAGSGPGVTVGAG
                     ETVLEVTGEGRGGRNTHAALLAATRIAGTNDVFVAFATDGVDGASGSAGAVVDGGTVA
                     RGGAPSDALGSFESAGYLEASSDLLRCSPTGTNVSDIWILWRREPVAT"
BASE COUNT          371 a          773 c          745 g          334 t
ORIGIN      
        1 gggccccgag tcgaaggtgc tgctgacgac cgagtcgaaa ggggccacac cacgtcttca
       61 cgacatcgcg gtgatgagtc gaatcgggct cgacctgagc ccgatcgacc ccggcgacca
      121 ggagtcacgt ctctggctcg acgcgctgat ctggcccgag catgtggagc gaagggcgag
      181 actgcgcgcc gctctcgagg tgatggcggc cgtcgaggtc gagatggtgg cgggcgacgc
      241 cctggtcacc ctccccgaga cgctggccgg tctccccgga gcggatccgg ccatcgtggt
      301 caactcgttc accctgattc agttcagccc cgagcagcga gaccaactcg aggagatcgc
      361 ggccgccgcg cgcgcacagc gcccggtgtt ccgggcgtcg atggaggccc tggacaagaa
      421 cgacgattgg gcgcggctcg tcatcgacga cgggtcggga ccgagacagg tcggcgaggc
      481 acacccccac ggggaatgga tcgacctgtc tgagcgctag gcccgaccgt agaccggtat
      541 ctcggcgccg ttgatgacac cggactcgtc gctgagcagg aagtgggcaa cggccgcgat
      601 ctcgtcaggc gtaggccagg tgacgtagtc ggcgtacgga agcgactgcc gcgtggcggg
      661 ggtgtcgatc accgacggca tgaggacatt ggcggtcacc ccggtcccgt cgagttcctg
      721 ggccaccgac ttggtgaggg cgaccacgcc cgacttcgcg atgttgaacg cgccctgccc
      781 ggtgggggcg tcgaccgcgg ccctgctccc tacggtcacg atccgtcccc agccggtctc
      841 caccaggtag gggacgaccg ccctcaccgc gtaaaaggcc gagcgaagat tgagatcgat
      901 catccgctcg aaacggacgt cgtcggtctc cgcgacgtcc cgccccccgg cccatccccc
      961 gaccagtgag cacagaccgt ggatcgaccc ccaccgatcg gccacgttct tcatcaggct
     1021 gttgacggcc tcggcgttgg tggcgtccac ccggaccagc agcaaccggt cctcgtcgat
     1081 gtcgaactcc tcctcgaact gagcggcttc ctcgggcatg aggtaggtga cggccagccg
     1141 gtagtcctcg aacacgagac gtcgcaccag cgcggaacca agaccaccgg tcccacctgt
     1201 tatgacgagt gtcttttcca tgctcccagc tctcggtcag ttcactctac gttgcgactg
     1261 gttctcggcg ccacagaatc cagatatccg agacgttggt cccggtgggg gagcaacgca
     1321 acaggtcgga actggcctcc agatagcccg ccgactcgaa ggaaccgagt gcgtccgagg
     1381 gggccccgcc gcgagccacc gtgccgccgt cgaccacggc tcctgccgag cccgaggccc
     1441 cgtccacgcc gtcggtggcg aatgcgacga agacgtcgtt tgtccccgcg atccttgtgg
     1501 ccgcgagcaa ggcggcgtgc gtgttgcgcc cgccccgtcc ttccccggtg acttcgagga
     1561 ccgtctcgcc ggcgccgaca gtgaccccgg ggccggagcc ggcgaagaac cgctcgagac
     1621 agggctcgac tggccccgcc agccaatccg acctgagcgc gaccggccct gacaccgcgc
     1681 tcgccacccc acgggcagca tccatcccgt tggcgagcag ggtgacctcg ggggctgcga
     1741 tcgcaggctg cggtgttcgc aacacggcga gcacctcatc gggaacatcg atcgcggccc
     1801 ttctcatgat cagagtcgcc atctcggcgt ccgcggctcg ggggatggtc ggccccgacg
     1861 ccacgacgcc agggtgggcc ccagcaacgt cggagatcac gtaggtgtcg atgggccggc
     1921 ctgcggcccg ggagaccccg ccgcccttga tcgccgagag atgggcccgg accaggttca
     1981 tctcttcgat gccggcgccg ccggcgatga gtcttctcgt cacctcggcg aggaactcgc
     2041 cgggcacccc gtcacgcggt ttctcgcaca gcgacgaacc gccacccgag atcaaggcga
     2101 tgatcgggat ggccggggac gcgtcttgca ccgcctccag gacagtctcg gcggcatgga
     2161 ggctggcggc gccgggcacg ggatgatcac cgacgaggaa ttggagtcga tcggacgtcg
     2221 gct
//
LOCUS       DATITW010000405         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_581179, whole genome shotgun sequence.
ACCESSION   DATITW010000405 DATITW010000000
VERSION     DATITW010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581179"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VLS86_05705"
     CDS             <1..696
                     /locus_tag="VLS86_05705"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HSO50022.1"
                     /translation="QAAGVAAFPFLARLAAAGDEQELIRTTGRAARNTLFVATAATAA
                     LIVLARPMVRLIYQYGEFTSENTELVASLLVLYAFSIPAWGLHQILSRHFYAKRRMWT
                     VVLIGTAGSLIAIPIWLGLYSAMGVEGFALASSVVMSVYALALLVAWGFDSGWGPVRL
                     LLPSLFRGLLAAGVAVLVAYPLVQALFDDGDLSVIEGLGAAALGALTTLAAFLAVSYV
                     LRAPELSELRRKG"
     gene            complement(716..913)
                     /locus_tag="VLS86_05710"
     CDS             complement(716..913)
                     /locus_tag="VLS86_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50023.1"
                     /translation="MADAIEIRGGAGAVEAAVIAVVLDRIAEEERVAREGRGGDSSLS
                     AWMLAERPEQANVPLEIVRPT"
     gene            complement(906..1316)
                     /gene="mce"
                     /locus_tag="VLS86_05715"
     CDS             complement(906..1316)
                     /gene="mce"
                     /locus_tag="VLS86_05715"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HSO50024.1"
                     /translation="MKLLNLDHVAIAVNDLDAALSGYRERYRVEPLYREMVEAQGVEE
                     AMIPIGGSFVQLLQPLGPDTPVGRFLASNGEGLHHIAFAVASIDLALQHLEAEGARLV
                     DRSARPGGRGARIAFVHPADLTGTLIELVELSDG"
     gene            1397..>2022
                     /locus_tag="VLS86_05720"
     CDS             1397..>2022
                     /locus_tag="VLS86_05720"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546665.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HSO50025.1"
                     /translation="MSNPVITGIARTPIGRYNGALKQMRAVELGGVAMAAALERSLAD
                     PAAIDEVLFGHVLQAGEGQITARQAAVQGGLPMNVPSVTINKVCLSGMSAIGLAERGI
                     RAGDTKFAMTGGMESMSNAPHVLRELRWGVRAGDAEMVDVMQHDGLFCAFDHCTMGES
                     SDRKNQRLGIGRQEQDEWAARSHQRAASATEQGLFADEIAPVLITQEKG"
BASE COUNT          322 a          684 c          658 g          358 t
ORIGIN      
        1 caagccgccg gagtggcggc tttcccgttt ctggcccggc tcgccgccgc cggggacgag
       61 caggaactca tccgcaccac gggacgggca gctcgcaaca cgctgttcgt ggcgacggcc
      121 gcaaccgccg ccctgatcgt ccttgcccgc cccatggtcc ggctcatcta ccaatacggc
      181 gaattcacct cggagaacac cgaactggtc gccagcctct tggtcctcta tgccttctcg
      241 ataccggcat ggggcctgca ccagatcctg tcccgccatt tctatgccaa gcggaggatg
      301 tggaccgtgg tcctcatcgg gaccgcgggg agcctgattg ccattccgat ctggctcggt
      361 ctctactccg caatgggggt cgaggggttt gccctggcct cgtcggtggt gatgagcgtc
      421 tatgcccttg ccctgctcgt ggcctggggt ttcgactcgg gctggggacc ggttcggttg
      481 ctcctccctt ccctgttccg gggattgctc gcggccgggg tagcggtgct ggtcgcctat
      541 ccgctggtgc aggcgctatt cgacgacggg gacctctcgg tgatcgaggg cctgggggcg
      601 gcggcgctcg gcgccctgac cacgctggcg gcgtttcttg ccgtttccta tgtcctgcgg
      661 gcccctgagc tgagcgagct gcgacgtaag ggctgacccc gggtctgccc cgtgatcagg
      721 tggggcggac gatctccagc gggacatttg cctgctcggg gcgctcggcc agcatccagg
      781 cggagagcga cgaatcgccc cctcgaccct cgcgagccac ccgctcctcc tcggcgatgc
      841 ggtcgagcac cactgcgatc acggctgcct cgacggcgcc ggctccacct ctgatctcga
      901 ttgcatcagc catccgacaa ctccaccaac tcgatcaagg tcccggtcag gtcggccggg
      961 tgaacgaatg ctatgcgcgc cccgcgcccg ccgggacggg ccgacctgtc gaccagtctc
     1021 gcgccttcag cctcgagatg ctggagagcg agatctatcg acgccacggc aaaggcgatg
     1081 tgatgcagtc cctcaccgtt actcgccaga aagcgcccca ccggggtgtc cggccccagt
     1141 ggctgcagca gctgcacgaa tgagccgcca atcgggatca tcgcctcttc gacaccctgt
     1201 gcctccacca tctcccggta caacggctcc acccgatagc gctcccgata gccggagagc
     1261 gcggcgtcga ggtcgttgac cgcgatggcc acatgatcca ggttgagcag cttcaccggc
     1321 agaggctatt gccgctgcgg agcggtgtca cctgctgccg gtaacatcga aagctcagga
     1381 ggtctctgtt ccccccatga gcaaccccgt catcaccggg atcgcccgga ccccgatcgg
     1441 tcggtacaac ggcgcgttga aacagatgcg ggcggtggaa ctgggtggtg tcgccatggc
     1501 ggccgccctg gaacggagcc tggccgatcc cgcagcaatc gacgaagtcc tctttggcca
     1561 tgtcctccag gccggtgagg gtcagatcac ggctcggcag gcagcagtgc aaggtggcct
     1621 gccgatgaac gtcccttcgg tcacgatcaa caaagtgtgt ctctcgggga tgtcggccat
     1681 cgggctcgcc gagcgaggga tccgcgctgg tgacaccaag ttcgcgatga ccggaggcat
     1741 ggagagcatg tccaacgccc ctcatgtcct gcgcgagttg aggtgggggg tgcgggcggg
     1801 agacgccgag atggtggatg tgatgcagca cgacggcctc ttctgcgcct tcgaccactg
     1861 cacgatgggt gagtcctccg accgcaagaa ccagcgtctc ggcatcgggc gccaggagca
     1921 ggacgaatgg gccgcccgca gccatcagcg agccgcctcg gcgacggagc aaggtctctt
     1981 cgccgacgag atcgctcccg tcctgatcac ccaggagaag gg
//
LOCUS       DATITW010000406         1559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_621091, whole genome shotgun sequence.
ACCESSION   DATITW010000406 DATITW010000000
VERSION     DATITW010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621091"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VLS86_05725"
     CDS             complement(<1..906)
                     /locus_tag="VLS86_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HSO50026.1"
                     /translation="MLKRLRQRRDDRGAALIWIAGTLVILLSMAAFATDLGWILLWNA
                     RLQAAADSAALAGVVNLPGFPAQARADAELAAGANRFPIPGSATLTDQVIAANEYRVT
                     LEADVDTFFLGLIGFDTFHLSQMAHAEYIKPVRLGSPFNQFGDESGFLWAAINGQFTE
                     IQQGDPFASECITHAADTPGCAVATSDGSYRPGGYYYGIEVAPGSSNLDVSIFDGGHY
                     VDDTGAGTSIADDHSWRWNWPDGQRGVELEYNLFEPDSSPTNPEDNNVLRCTGSFPVN
                     GPNNEGHFDSWGGNNNCSVGGSLTPG"
     gene            complement(906..>1559)
                     /locus_tag="VLS86_05730"
     CDS             complement(906..>1559)
                     /locus_tag="VLS86_05730"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HSO50027.1"
                     /translation="IPGSRRLHDLGDGKGTGVLRKWRQATGLGQRGSVAPRERGAALT
                     EMALLLPILLLLVIGVFELGGAFKDYLTTSNAVRDGTRILSATGTQPTSDCVALVRAV
                     DSMSTATRLDSLVSIQIFQANDSGDPIPGSINTYTYASGDPATCTPTAPNCGSWNCTI
                     QQPPSSRSALVGSGVSPDLIGMRIIYTHNWYTGFPPFSGSLTIDEQTISRLEPEGLA"
BASE COUNT          273 a          458 c          542 g          286 t
ORIGIN      
        1 ccctggggtc agggagccgc ccaccgagca gttgttgttt ccaccccagc tgtcgaagtg
       61 gccctcattg ttaggcccgt tgaccgggaa ggagccggtg cagcgcagca cgttgttgtc
      121 ttcggggttg gtggggctgg aatcaggctc gaacaggttg tactcgagtt ccacaccgcg
      181 ctgaccatcg ggccagttcc agcgccatga gtgatcgtcg gctatcgagg tcccagcacc
      241 ggtgtcgtcc acatagtgcc caccgtcgaa gatgctgacg tccaggttgc tcgaaccggg
      301 ggcgacctcg atcccgtagt agtagccgcc aggtcggtag gacccgtccg acgtggcaac
      361 tgcgcacccc ggggtgtcgg cagcgtgggt gatgcactcc gaggcgaagg ggtcaccctg
      421 ttggatctcg gtgaactggc cgttgatcgc ggcccacagg aacccgctct cgtcgccgaa
      481 ctggttgaac gggcttccca acctcaccgg cttgatgtac tcggcgtggg ccatctggga
      541 gagatggaag gtgtcgaaac cgatcagacc gagaaagaag gtgtccacat cggcttcgag
      601 tgtcacccgg tattcattcg ctgcgatcac ctggtcggtg agagtggcag agcccgggat
      661 cgggaaccgg ttggccccgg ccgccaattc ggcgtcggcg cgggcttgcg cggggaaccc
      721 ggggaggttc accactccgg cgagggcagc ggaatcggct gcggcctgga gtctggcgtt
      781 ccagagcagg atccagccga gatcggtggc gaacgcagcc atgctgagca ggatgacgag
      841 ggttccggcg atccagatca gggccgcccc tctgtcatct ctgcgttggc ggagacgctt
      901 gagcatcagg cgaggccctc tggctcgaga cgggagatgg tctgctcgtc gatggtgaga
      961 ctcccgctga atggggggaa tccggtgtac cagttgtggg tgtagatgat tctcattccg
     1021 atcaggtcgg gggacacgcc gctgcccacc agagcgctcc gggaggacgg aggctgttgg
     1081 atggtgcagt tccatgatcc acagttgggg gcggtggggg tgcaagtcgc gggatctccg
     1141 gaggcatagg tgtaggtgtt gatcgagcca ggaatcggat ctccggagtc gttggcctga
     1201 aagatctgga tcgacaccag gctgtcgagc cgggtcgcgg tcgacatcga atcgacagcg
     1261 cggaccagcg ccacgcagtc agatgtcggc tgggtgccgg tggcgctgag gatgcgggtc
     1321 ccgtctcgaa ccgcgttgga agtagtcagg tagtccttga aagctccacc gagctcgaag
     1381 accccgatca ccaggagcag gaggatcggg agcaggagag ccatctcggt gagcgccgct
     1441 ccccgttccc ggggcgcgac cgatccacgt tgccccaacc cagttgcttg acgccacttc
     1501 ctcaacacgc ccgtcccctt gccgtccccg aggtcgtgaa gcctccttga cccgggaat
//
LOCUS       DATITW010000407         1552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_60408, whole genome shotgun sequence.
ACCESSION   DATITW010000407 DATITW010000000
VERSION     DATITW010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60408"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VLS86_05735"
     CDS             <1..410
                     /locus_tag="VLS86_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HSO50028.1"
                     /translation="PVADANEAVSEADVISCATPATQPLFSESVVRESVHVNAIGAFT
                     PEMVELPPELLERAYVVVDDIGAAAAEAGDLIQAGRAPNTSLREMLAGTSPEIGEDVT
                     VFKSVGVAAQDVAAAHLALVNAERIGLGTLLPG"
     gene            complement(444..659)
                     /locus_tag="VLS86_05740"
     CDS             complement(444..659)
                     /locus_tag="VLS86_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HSO50029.1"
                     /translation="MCRSIKVLREGSTPAPSEEIEAAALQFVRKISGFSKPAHHNEDV
                     FNQAVVEISEASQRLLDSLEIRGAKAS"
     gene            complement(728..>1552)
                     /locus_tag="VLS86_05745"
     CDS             complement(728..>1552)
                     /locus_tag="VLS86_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50030.1"
                     /translation="FRSHITRPLDPTNPWNRAVILISVLVGIVGAILTVVSDRELMLA
                     IQAGGTTFLSWAVVRELDPDRQASAIVAGVVGGAWALMGQPTALLPFLGLLMISRLLV
                     ETTGRRPLATDLAGMAVLATVISYTPLGWVMGFGLGISIYIDDRLAEEPNRQALLAAM
                     AAGVGSSVVVTLVDALPGALPSVRPLLAALIGLLALIAVMREPVDPVSFVDSRNKRFL
                     RRDRLQVARSVAGVLLFIAAFFSGESATAVVPMAFCLLVALASTEVERVQRAHHTP"
BASE COUNT          280 a          498 c          538 g          236 t
ORIGIN      
        1 aaccggtggc ggacgccaac gaggcggtgt ccgaagcaga cgtgatctcc tgtgccacac
       61 ccgccaccca gcccctgttc tcggagtccg tggtcagaga gtctgtccat gtgaacgcga
      121 tcggggcatt cactcctgag atggtcgagc tgccccctga gctgttggaa cgggcctatg
      181 tcgtagtcga cgacatcggg gctgctgccg cggaggcagg ggacctgatc caggcgggtc
      241 gggcgccgaa caccagtctg agggagatgc tcgccggcac cagccccgag atcggcgagg
      301 acgtgacggt gttcaagagt gtcggggttg cggcccagga tgtggcggcg gcccatctcg
      361 ccctggtcaa tgccgagcgg atcgggctcg ggaccctgct tcccgggtag tcggcccatt
      421 tcaccccaaa gggccctttg cggttaggac gccttggcgc ccctgatctc cagcgagtcg
      481 agcagtctct gtgacgcttc gctgatctcg acgaccgcct ggttgaagac gtcctcgttg
      541 tgatgcgccg gcttgctgaa gccggagatc ttgcgcacga actgcagggc ggccgcctcg
      601 atttcctctg agggggccgg cgtcgacccc tctctcaata ccttgatcga tctgcacatg
      661 cggacaactt ataccacctc ccgtaattgg gagccgcagg tccggatatg accccggtgg
      721 ctcccaatta cggggtgtgg tgggcccgct gcacccgctc cacctcggtg gaggcgaggg
      781 cgacgagcag acagaaggcc atggggacca ccgctgtcgc cgactcgccg gagaagaagg
      841 cggcgatgaa caggaggacg ccggcaacgg atctggccac ttggagacgg tcgcggcgaa
      901 ggaacctctt gttccggctg tcgacgaagc tgaccgggtc gaccggttct ctcatcaccg
      961 caatcagggc gagcaagccg atgagagccg caaggagggg gcggacggag gggagagccc
     1021 cgggcagggc gtcgaccagg gtcaccacca ccgacgagcc caccccggcc gccatcgcag
     1081 cgagcagcgc ctggcggttg ggctcctccg ccagccggtc gtcgatgtag atcgagatcc
     1141 ccagaccgaa gcccatcacc cagcccagcg gggtgtagga gatgaccgtc gccaggaccg
     1201 ccattccggc caggtcggtg gccagggggc ggcggccggt ggtctccacc aggagccgcg
     1261 agatcatcag cagaccgagg aaagggagca gggccgtggg ttggcccatc aaggcccagg
     1321 ccccacccac gacccccgcc acgattgccg atgcctgacg gtcggggtcg agctcgcgga
     1381 ccaccgccca ggagagaaag gtggtgcccc cggcctgtat cgccagcatc aactcccggt
     1441 cggacacaac ggtgaggatg gcgccgacga tcccgacgag caccgagatc aggatcaccg
     1501 cccggttcca gggattggtg gggtcgaggg gtcgggtgat gtgagacctg aa
//
LOCUS       DATITW010000408         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_420806, whole genome shotgun sequence.
ACCESSION   DATITW010000408 DATITW010000000
VERSION     DATITW010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420806"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VLS86_05750"
     CDS             complement(<1..881)
                     /locus_tag="VLS86_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50031.1"
                     /translation="MNLSHPAIRVEGLVKRYKRTTALDGLDFQVPSGAITGFLGPNGA
                     GKTTTFRSLLGLTLVDAGQIEVLDHEMPQGLFEVTKKVGAIIEEPGLIRALSGRVNLQ
                     VAADQLGFGHDRIDGLLEFVELSHEAKRKVEEYSKGMRQRLALAAALIGEPELLLLDE
                     PLDGLDPAGQRAFRARLRDIADSGHTVVISSHDLADVEALADYVVVLNHGKLVAQGPL
                     EELLSGGATRVVTDDAEGARAALVAAGIAVQVDGEVLTVDEVDGRRVIRVLAEAGIYP
                     SEVRPARSTLESVFLGMT"
     gene            complement(916..1344)
                     /locus_tag="VLS86_05755"
     CDS             complement(916..1344)
                     /locus_tag="VLS86_05755"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HSO50032.1"
                     /translation="MGLGHVPAGPVPGAREGAHVPRRQRRRVGAARDLDLTDRARLGS
                     IGERVASRFLTDHGLRVVASNLDVGRGELDLLALDGQTRVVIEVRTISGSGDPIDAVD
                     ASKRRRVRTLAGRIGAGRVDFIGVGIAPAALVVHWVPGCG"
     gene            complement(1236..1532)
                     /locus_tag="VLS86_05760"
     CDS             complement(1236..1532)
                     /locus_tag="VLS86_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 family protein"
                     /protein_id="HSO50033.1"
                     /translation="MEEDLERYESEVELKIYKEYHDVLPMFRYVVETERRFYLANEVN
                     LEVREANGSVFFEVEMKDAWVWDMFRPARFLEHVKVLTFRDVNVEELEQREISI"
     gene            complement(1613..>2005)
                     /gene="lepB"
                     /locus_tag="VLS86_05765"
     CDS             complement(1613..>2005)
                     /gene="lepB"
                     /locus_tag="VLS86_05765"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSO50034.1"
                     /translation="GDVVVFRDPREEEIEETIPEAVIRSVLEAVGIRTRGHEDLIKRV
                     IGLPGETVEVKDNRVVINGTPLDEPYLVDVFMPDEPAITISADQVFVMGDNRNASFDS
                     RRFGPIPLDDLVGEAFVTIWPISHFGGF"
BASE COUNT          367 a          692 c          593 g          353 t
ORIGIN      
        1 gtcatgccga ggaacaccga ttccagcgtt gaccgagccg gtctcacctc actggggtag
       61 atcccagcct cggccaggac ccggatgaca cgtctgccgt cgacctcgtc gacggtcagc
      121 acctcgccgt cgacctgcac cgcgatcccc gccgcgacca gggcggcgcg ggcgccctcg
      181 gcgtcgtcgg tgacgacacg tgtggccccg ccggagagca actcctcgag tggcccctgg
      241 gcgaccagct tcccgtggtt gagcaccacg acgtagtcgg ccagggcctc gacatcagcc
      301 aggtcgtggc tggagatgac cacggtgtgc ccgctgtcgg cgatgtcgcg gagccgggcg
      361 cggaacgccc gttgccctgc cgggtcgaga ccgtccaggg gctcgtccag cagcagtagc
      421 tccggctcgc cgatcagggc cgcggccagg gccagtctct gccgcatccc cttcgagtac
      481 tcctcgacct tccgcttggc ttcgtgggag agctcgacga actcgagcag gccatcgatc
      541 cggtcatgac cgaatccgag ctggtcggcc gcgacctgga gattgacccg gccactgagg
      601 gctcgaatca accccggctc ttcgatgatc gcccccacct tcttcgtcac ctcgaacagc
      661 ccctggggca tctcgtggtc caggacctcg atctgaccgg cgtcgacgag ggtgagaccg
      721 agcagagagc ggaacgtcgt ggtcttccct gcgccgttgg ggccgagaaa cccggtgatc
      781 gctcccgacg ggacctggaa gtcgagacca tcgagcgcgg tcgtcctctt gtagcgcttg
      841 accagaccct cgactctgat cgccgggtgg ctcagattca tgttctagaa caatagaggt
      901 gcatcgagca gcgagtcaac cacacccggg gacccagtgg acgaccaaag ccgctggggc
      961 gatcccgacc ccgatgaagt cgaccctgcc cgctccgatc cggccggcca gcgtccgcac
     1021 ccgacgacgc ttcgaagcat ctacggcgtc aatgggatca ccactaccgc tgatcgtcct
     1081 cacctcgatc acaaccctgg tctggccgtc gagggcgaga agatccagtt ctccgcgtcc
     1141 gacgtcgaga ttggaggcga ccaccctgag cccgtgatcg gtcaggaagc gggatgcgac
     1201 acgctcaccg atgctcccga gacgggcccg gtcggtcaga tcgagatctc gcgctgctcc
     1261 aactcttcga cgttgacgtc gcggaacgtg agcaccttca cgtgctccag gaaccgggcc
     1321 ggccggaaca tgtcccaaac ccatgcgtcc ttcatctcga cttcgaagaa gacggatcca
     1381 ttggcctctc gcacctcgag gttcacctca ttggccaggt agaaccttcg ctcggtctcc
     1441 accacgtacc ggaacatcgg gaggacgtcg tggtactcct tgtagatctt gagctcaacc
     1501 tcggattcgt agcgctccag gtcctcttcc atcttctcct cttcacacgt gacgcccagc
     1561 gatcttatgc acgcctcgtg cgctaatacc taacagatgg ttgagacatt tctcagaatc
     1621 cgccgaaatg ggaaatgggc cagatggtga cgaaagcctc tccaaccagg tcgtcgagag
     1681 ggatggggcc gaaccgacga ctgtcgaacg acgcattccg gttgtccccc atcacgaaca
     1741 cctgatcggc agagatggtg atcgccggct cgtcgggcat gaagacatcc accagatagg
     1801 gctcatcgag gggagtgccg ttgatcacga cgcgattgtc cttgacctcc acagtctccc
     1861 cgggcagccc gatcacccgc ttgatcaggt cctcatgccc gcgggtgcgg atcccgacgg
     1921 cttcgagaac ggagcggatc accgcctcgg ggatcgtctc ctcgatctcc tcctcccgcg
     1981 ggtcccggaa cacgaccaca tcgcc
//
LOCUS       DATITW010000409         1676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_220477, whole genome shotgun sequence.
ACCESSION   DATITW010000409 DATITW010000000
VERSION     DATITW010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220477"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1676
                     /locus_tag="VLS86_05770"
     CDS             <1..>1676
                     /locus_tag="VLS86_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HSO50035.1"
                     /translation="SKAEASLAALEKTMKTIARVRRNGDAVEVDAADLVPGDIVLIEA
                     GNMVPADGRLFVAATLEIEEAALTGESTPTLKSTDVLETPELGLGDRVNMAFMQTAVT
                     RGRGEMIVTGTGMNTEIGHIAELLNKTDADKTPLQKQLDRLTVLIAGLAGLAFLAMVL
                     LGLANDQELETIFVSGVALAISAIPTGLPAVVTTMYSMGTRALAAINAIVKRLPSVET
                     LGSVSAICSDKTGTLTLTQMTAVEFSVPGQNRYTVKGEGYSTIGKITHAGGAVLPLDD
                     VMLPLALCADARLDGESLIGDPTEGALIVLAEKGGIDVDAAREMYPRVAEVPFDSEYK
                     FMATFHEMTDDDGHQVVRCLVKGAPDVLISRGGYYWQPGGAAVPITDENRAMALAENE
                     RMAAGGKRVMVVARRDFDPRSFDPNANLIDEMKELTLLAMVGIVDPPRAEARDAIARC
                     HSAGIQVRMITGDHAVTAAAIGGQLGIEGTALTGAQFAAMSDDELTGRLDEIGVVARV
                     APEDKIRLVDMLQKKEHIVAMTGDGVNDAPALKKADIGVAMGITGTEVSK"
BASE COUNT          314 a          543 c          564 g          255 t
ORIGIN      
        1 gtcaaaggcc gaggccagcc tggccgcgct ggagaagacg atgaagacca tcgcccgggt
       61 gcgacggaac ggcgatgccg tcgaagtcga cgccgcggac ctggtccccg gggacatcgt
      121 cctgatcgag gcgggcaaca tggtccccgc cgacgggcgt ctgttcgtcg ccgccacact
      181 cgagatcgaa gaagcggcgc tcaccgggga gagcacaccg actttgaagt cgaccgacgt
      241 cctcgagacg cctgagctgg gattgggtga ccgggtcaac atggctttca tgcagaccgc
      301 ggtgacccgg ggccggggtg agatgatcgt gaccggcacc ggaatgaaca ccgagatcgg
      361 gcacatcgcc gagctgctaa acaagaccga cgccgacaag actccgctgc agaagcagct
      421 cgaccggctc acggtgttga tcgcgggtct ggcgggcctg gcgttcctgg ccatggtgct
      481 gctcggcctc gccaacgacc aggagctgga gacgatcttc gtctcgggtg tggctctggc
      541 catttccgcc atcccgaccg gcctcccggc cgtcgtcacg acgatgtact ccatggggac
      601 cagggccctg gccgcgatca acgccatcgt caagcggctc ccctcggtgg agacactggg
      661 ctccgtctcc gccatctgct ccgacaagac cggcaccctg accctgaccc agatgaccgc
      721 ggtcgagttc tcggtcccgg gtcagaaccg ctacacggtc aagggggagg gctacagcac
      781 catcgggaag atcacgcatg ccgggggggc ggtgctgcct ctcgatgacg tgatgctccc
      841 cttggcgctc tgcgccgacg ccagactcga cggagagagc ctcatcggcg accctacaga
      901 gggggcactc atcgtcctgg ccgagaaggg aggcatcgac gtcgacgccg cgcgggagat
      961 gtacccgcgg gtggccgagg tgccgttcga ctccgagtac aagttcatgg ccaccttcca
     1021 cgagatgacc gacgacgacg gccaccaagt cgtgcgctgc ctagtcaagg gagcaccgga
     1081 tgtgctcatc tcccgcggcg gctactactg gcagccgggc ggggccgccg ttccgatcac
     1141 ggacgagaac cgggcgatgg cccttgccga gaacgagcgc atggccgccg gtggtaagcg
     1201 tgtgatggtc gtggcccgcc gcgacttcga ccctcgatcg ttcgatccca acgcaaacct
     1261 catcgacgag atgaaggagc tgactctcct ggccatggtg ggcatagtgg acccgcctcg
     1321 tgccgaggcc agggacgcca tcgccaggtg tcatagcgcc ggcattcagg tgcgcatgat
     1381 caccggcgat catgcggtga ccgccgctgc gatcgggggc caactgggca tcgaggggac
     1441 ggctctgacc ggcgcccaat tcgccgcaat gagcgacgat gagctgaccg gccgactcga
     1501 cgagatcggc gtggttgcca gagtcgcacc cgaggacaag atccgtctcg tcgacatgct
     1561 gcagaagaaa gagcacatcg tcgccatgac cggtgacggc gtgaacgatg cgccggctct
     1621 taagaaggcc gacatcgggg ttgccatggg catcaccggc accgaggtct cgaagg
//
LOCUS       DATITW010000410         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_340429, whole genome shotgun sequence.
ACCESSION   DATITW010000410 DATITW010000000
VERSION     DATITW010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340429"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..630
                     /locus_tag="VLS86_05775"
     CDS             235..630
                     /locus_tag="VLS86_05775"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSO50036.1"
                     /translation="MTDRPFSVVASSGDLRPSPHAGVEFGHHWTPEGVVAQSDFTGAH
                     LLHLSVAGCVLNDVHREAERLGLTVDGVRVAAWGDFDRETWQSTGISYSVEVSSPAPT
                     AGIEELLRIVDDVAEIPKALRSEATVERR"
     gene            complement(798..870)
                     /locus_tag="VLS86_05780"
     tRNA            complement(798..870)
                     /locus_tag="VLS86_05780"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(834..836),aa:Glu,seq:ttc)
     gene            complement(981..>2067)
                     /gene="gltX"
                     /locus_tag="VLS86_05785"
     CDS             complement(981..>2067)
                     /gene="gltX"
                     /locus_tag="VLS86_05785"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HSO50037.1"
                     /translation="LEALRQRAQEERLHPGHYVRRPDVPATSGVIRMSIPQGESVVFD
                     DLVRGTVSFEGRDIDDFVILRSDGTPTYHLASTVDDVDYEITHVARGEDLLPSTPKHI
                     LLTRALGAAEVVYAHLPLLFGPDGKKLSKRHGETSLSHYRDTGYLPDAVFNYLSLLGW
                     SIDGDTTIFSRDEAISAFDLADVSSSPAVFDPDKLNWLNGEYIRAMAPSRFLEEVKPH
                     VESAVGRPLSQEEWARFEAISDLVQERTRLLPEAGAQVVFLFADFEEYDPSAWEKVMA
                     KDGVAGILDAARERLEGIEEWSAEVVEPVLRAMLEEMGIGTGKGLQPLRVAVTGSSVS
                     PPLFESMSALGKEKTLQRLQRARALLG"
BASE COUNT          365 a          661 c          671 g          370 t
ORIGIN      
        1 gaacatgaga ccccctaccc atcccatgga cttcatcagc gggcgaagcg ctttggggaa
       61 ttcgacacca cgggtgccgc tgggaggcag gtcgaccatc aatcgaaccg tacctggctg
      121 ggtgcggccg tcgtgaggct ggccatcatc ggcatcggta tcaaggcgct cagacctcca
      181 ccgacacccc gaccggcaaa tacgggccca ccgtgatcat tagcttctgg ccatatgacc
      241 gatagaccct tttcggtggt tgcgtcctct ggcgaccttc gaccctcccc acacgctggt
      301 gtcgagttcg gccaccattg gacgccggaa ggggtagtgg cgcagtcaga tttcacgggc
      361 gcacatctcc tgcatctctc tgttgctgga tgcgtcctga atgacgttca tcgggaagcc
      421 gagcgcctcg ggttgactgt tgacggtgtg agggtcgccg cttggggtga cttcgaccga
      481 gagacgtggc agtcaaccgg gatcagctac tccgtggagg tgagctctcc agctccgact
      541 gccgggatcg aagaactgtt gaggatcgtt gacgacgttg cagagatccc caaggctctg
      601 cgatcagagg caaccgtcga gcgcagatag cggtcgggaa aggctgccct tcagatctgg
      661 cgtcagcccg cgccgatctg cattaggagc gtcgggtcaa atgcgtgtct ccaaccactg
      721 gagcgccttc tgcaggtgag gagtgaaggg ctcgggatac tcgtggctca tggagcactc
      781 cgacgaggga gatgttgcag tcccgaccgg attcgaaccg gcgtttccgg cttgaaaggc
      841 cggcgtccta ggccactgga cgacgggacc tctggaacca gcaggttacc tgctcggcgg
      901 gtgattactc cctccggccc tctggcgccc ccgcctcttc gcgagcacaa ggccgtcaag
      961 cctgccgtca gaccgaaagc tcagccgagc aaggcccgcg cccgctgcag acgctgcagg
     1021 gtcttctcct tgcccagggc ggacatcgac tcgaacagcg gcgggctgac cgacgaaccg
     1081 gtgactgcga cccggagcgg ctgcaaaccc ttgccggtgc ctatccccat ctcctccagc
     1141 atggccctca acaccggctc gacgacctcg gcactccatt cctcgatgcc ctcgagtcgt
     1201 tcccgtgctg cgtcgaggat gccggcaacg ccgtccttgg ccatcacctt ctcccaggcc
     1261 gacgggtcgt actcctcgaa gtccgcgaag aggaagacca cctgggcccc ggcctcgggg
     1321 agcagtcggg tccgctcctg aaccagatcc gagatcgcct cgaacctggc ccattcctcc
     1381 tggctcaggg gtcgacccac ggctgactcc acatggggct tgacctcttc gaggaaccgg
     1441 gagggtgcca tggcacggat gtactcgccg ttgagccagt tcagcttgtc tgggtcgaac
     1501 accgccgggc tgctggagac atcggcgaga tcgaaggccg agatcgcctc gtcccgggag
     1561 aagatcgtcg tatcgccgtc gatcgaccag ccgagcaggg agagatagtt gaacacggcg
     1621 tcgggcagat agccggtgtc gcgatagtgg gacagcgagg tctcgccatg ccgcttcgac
     1681 agcttcttgc cgtcgggccc gaagagcagc gggagatggg catacaccac ctcggcggcg
     1741 ccgagggccc tggtcagcag tatgtgcttg ggggtggagg gcaacaggtc ctccccgcgg
     1801 gcgacgtggg tgatctcata gtcgacgtcg tccacggtgg aggccaggtg ataggtgggg
     1861 gtgccgtcgc tgcgcaggat cacgaagtca tcgatgtctc ttccctcgaa gctgaccgtg
     1921 ccccggacca ggtcgtcgaa gacgacgctc tccccctggg ggatgctcat ccggatcacg
     1981 ccgctggtgg ccgggacgtc ggggcggcgg acgtagtgac cgggatgcag gcgctcctcc
     2041 tgcgcccgct ggcggagagc ttccaac
//
LOCUS       DATITW010000411         1801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_300504, whole genome shotgun sequence.
ACCESSION   DATITW010000411 DATITW010000000
VERSION     DATITW010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300504"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VLS86_05790"
     CDS             complement(<1..786)
                     /locus_tag="VLS86_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSO50038.1"
                     /translation="MIKPDYTGGGLANLVTELECRLNGKETGVALRSELATQIPDART
                     YVLLLVDGLGIDQLDHPAAEAMNAAKRGMLHAPFPTTTSVSLATVATGRPPSEHGLVS
                     HLAWLEEAGGVVNTLKWVDMAGLPVAHDYASVLPKHNLWERLRAAGVEPITVQPGSFE
                     GTPLSRLLYRGARFEPTWDTSDLIDATVQLADSGRRFVFTYVWQVDFAAHVNGLGSSE
                     FDDAMSLAARVWDSLASRLPADVALIGTADHGLVGFSEDDKILV"
     gene            846..1316
                     /locus_tag="VLS86_05795"
     CDS             846..1316
                     /locus_tag="VLS86_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSO50039.1"
                     /translation="MITATGVMDNEQVYRSYAVELTKYATGLVGPFDAADVVTDACLK
                     AFSSRSWPEVTNRRAYLYRVVLSVATDHHRSTLSRRLRELKTAVPETASPQEVDVDVL
                     EAVERLSMQQRAAVLLTYWADLPVETVAIQMGVSTGAVKRHLARARKHLKESLS"
     gene            1337..>1801
                     /locus_tag="VLS86_05800"
     CDS             1337..>1801
                     /locus_tag="VLS86_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50040.1"
                     /translation="MKDLADRLVAMSPEPPPFPEEYIVTQPTNGSKQQSPVLIFAGAA
                     AIVIVLALIPILLLRGGGEVAPLGTTTTSTVPSTQSSEPLVTSTSIAEVTTTVPETTT
                     VTVAETQGVFVFLVQTPENSFLGNPALTPFFAMAEAQPGDPQVLSALRLLTAD"
BASE COUNT          342 a          570 c          572 g          317 t
ORIGIN      
        1 gacgaggatc ttgtcgtcct cggagaaccc gaccaaaccg tgatcagcgg tcccgatcag
       61 ggcaacgtcg gccgggagtc tcgaggccag gctgtcccag accctggccg ccagagacat
      121 tgcgtcatcg aactcggatg agcccagacc gttgacgtgg gcggcgaagt cgacctgcca
      181 tacgtaggtg aatacgaatc gccgtccgct gtcggccaac tgcaccgtgg catcgatcag
      241 atcggaggtg tcccaggttg gctcgaaacg ggcgccccga tagaggaggc gggacaacgg
      301 agtgccctcg aacgagccgg gttggacggt gatcggctcg acacctgcgg ctcgcaggcg
      361 ctcccacaga ttgtgcttgg ggagcacgga cgcgtagtcg tgggcaacgg ggagacctgc
      421 catgtccacc catttcaggg tgttgaccac tcctccggcc tcctcgagcc aggcgaggtg
      481 agaaaccagt ccatgctcgg agggagggcg accggtggcc acggtggcca gggagacgct
      541 ggtggtggtg ggaaacgggg cgtgaagcat gccccgtttg gcggcgttca tggcttcggc
      601 cgccgggtga tcgagctggt cgatcccaag gccatcgacc aacaacagca cgtaggtgcg
      661 agcgtcgggg atttgggttg ccagctcgga ccggagagcg acacccgttt ccttcccgtt
      721 gagacgacat tccaactccg tcaccagatt tgccaggccg cctccggtgt agtcgggctt
      781 gatcatcaac aggcaggcta ttgcgagctg tcacccaatc cgccggttgg ctccgtctca
      841 ggtacatgat caccgccaca ggtgtgatgg acaacgaaca ggtatatcgg tcatacgccg
      901 tcgagctcac caagtacgcg accggcttgg tggggccctt tgacgctgcc gacgtggtga
      961 ccgatgcctg tttgaaggcc ttttcgtcga ggagttggcc cgaggtgaca aaccgaaggg
     1021 cctatctcta tcgagttgtg ctcagtgtgg cgaccgacca ccaccggagc actctctccc
     1081 gccggctcag agagctgaag acggcggttc ccgagacagc ctcgccccag gaggtcgatg
     1141 tcgacgtctt ggaggcggtg gaacggctct cgatgcagca gagggcagcc gttctcctca
     1201 cctactgggc tgatctcccg gtcgaaacgg tcgcaataca aatgggtgtc tccacgggag
     1261 cggtgaagcg tcacctcgct cgcgcccgga agcacctcaa ggagtcgctg tcatgagcgg
     1321 aaccgacgac cagagaatga aagatcttgc cgaccggctg gtggccatgt ccccagagcc
     1381 gccgccattc ccagaggagt acatcgtgac ccaaccaacc aacggctcga agcaacagag
     1441 ccctgtcctg atcttcgccg gagctgccgc gatcgtgatc gtgcttgccc tgatcccgat
     1501 tcttctgctt cgcggaggcg gtgaggtggc gcccttggga acgaccacga cctcgaccgt
     1561 gccttccacc cagtcgagcg agccgctggt gacgtcgacc tcgattgccg aggtcaccac
     1621 aaccgtgccc gagaccacga ctgtgacggt cgccgagacc caaggtgtgt tcgtcttcct
     1681 ggtgcaaaca ccggagaact cattcctcgg caacccggcg ctcacaccgt tcttcgcgat
     1741 ggccgaggcc cagccgggcg atccccaagt gctgtccgca ctccggctcc tcacggccga
     1801 c
//
LOCUS       DATITW010000412         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_100719, whole genome shotgun sequence.
ACCESSION   DATITW010000412 DATITW010000000
VERSION     DATITW010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100719"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VLS86_05805"
     CDS             <1..191
                     /locus_tag="VLS86_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSO50041.1"
                     /translation="RGAHRLNAVTPGMGIGLAIVQAIAEAHGGRAEYGRSDRLGGACF
                     TLTIPGCVVRQGAVSVRS"
     gene            complement(223..2583)
                     /locus_tag="VLS86_05810"
     CDS             complement(223..2583)
                     /locus_tag="VLS86_05810"
                     /inference="COORDINATES: protein motif:HMM:NF045027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HSO50042.1"
                     /translation="MKANPRTLALLAATLLFSLLPVLPQTAQAADPWLDEDFEGGVGG
                     YSGWSLHPTSDGHVGDGAVSNIPAGQHWGSTGHWNTSSRLSSDPQEMWMRYWVRFPVG
                     FRVDPPSRGKLPGFAGLYSGNCRGNRPSTSGAPCWSARMSFSPIYNGDGLPTIPYDPA
                     RVTRVGWYAYLLSSGGAGKSGEILNWDPNLSTLQHGKWYCIEGRVKMNALGESNGILE
                     GYVDGNQAFNATNLTFRRPAESHLRVKSLWFDVYYGGDATSPKSNSISFDSLAAGPER
                     IGCNDQPGSSGTFFDDDGSVFENAIEQIAASGITVGCNPPANDRFCPDDSVTRGQMAA
                     FLKRALGDQYPITTPEPPPSPPDFFGGRSHLEYKTALAVYASGGAPFDTYGVSYWIDD
                     TTGDRDWLATGGNNTGNPNNGVPIQLGNIWNGGATPYVRIVAHDLRGLVAGSHDKRLN
                     NMLATFKRFTDLGGGRRLILDILPDANVKSSDYGDDPSRFRTAFRDIATRARVLLGDN
                     VRIAFSASRAMTSNHYSPGEWGTGGHRLYWPGSAHVDLAGVTGVASSGGSNAGLFETA
                     LGEMSNAAGPGVPLFIAAGGAPNAPSEAAQIAYVQALADLAVSNAQVVGVQWDDMLKG
                     SLDLRVSTSSGLQSGFATASQGARNGGVDWLFSSSVDAWAAARNAAHPFDDSTSSVFA
                     DSIRWLSATGITEGCAPRRFCPDDRVTRGQMAAFLGRALKLPAPPSPIVFTDARGHLF
                     EGAISRLAYAGITVGCNPPTNNRFCPDSFVTRGQMAAFLVRAGLTD"
     gene            complement(2705..>3433)
                     /locus_tag="VLS86_05815"
     CDS             complement(2705..>3433)
                     /locus_tag="VLS86_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HSO50043.1"
                     /translation="DPASHTYVSGKRSYAGEVGNVSFHHELGPDVSQGELEELIDTLN
                     RDDSVDGILVQLPLPGDLDSERIVERISPAKDVDGLHPHNLGLLVLDRPGLRPCTPSG
                     IMRILEHHGLSVSGKTAVVIGRSYLVGRPLALMLASKGVDATVTMAHSRTTDLAEVAR
                     AADILVAAAGVPGLVTADFVKPGAAVIDVGISRTEHGLVGDVSFESVRQVAGSITPVP
                     GGVGPMTRAMLLSNTLEAARARRG"
BASE COUNT          604 a         1112 c         1164 g          553 t
ORIGIN      
        1 aacgaggcgc ccaccgactc aacgcggtga cgccgggaat gggcatcgga cttgccatag
       61 tccaggccat cgcggaagcg cacggcggga gggcggagta cggccgatcc gaccgattgg
      121 gtggcgcctg cttcaccctc accatccccg gatgtgtggt ccgccagggc gcagtctccg
      181 tccgcagctg agtggtgccg ccggcagacc gccggccctg cctcagtcgg tgagaccggc
      241 cctcaccagg aacgcagcca tctggccacg ggtcacgaag ctgtccgggc agaagcggtt
      301 gttggtcggt gggttgcatc ccaccgtgat cccggcatag gccagacgtg agatcgcacc
      361 ctcgaacaga tggcctctgg cgtcggtgaa gacgatgggg cttggtggcg ccgggagctt
      421 gagggcacgg ccgaggaagg cggccatctg gcccctggtc acccggtcgt cgggacagaa
      481 gcgtcgcggg gcgcatcctt ctgtgatccc ggtggcgctc aaccagcgga tggagtcggc
      541 gaacaccgag gaggtggaat cgtcgaaggg gtgagctgcg ttgcgggccg cggcccacgc
      601 gtcgacgctc gaggagaaga gccagtccac gccgccgttg cgcgcccctt gcgaggctgt
      661 ggcaaagccc gattggagcc cggaggaggt gctgacacga agatcgaggg agcccttgag
      721 catgtcgtcc cactggaccc cgacgacctg ggcattggac actgccagat cggccagggc
      781 ctggacgtag gcgatctgtg ccgcctcgct gggggcgttg ggtgcccccc cggcggcaat
      841 gaagagaggg accccgggcc cggccgcgtt ggacatctcg cccagcgccg tctcgaaaag
      901 acctgcattc gagccgcctg acgaggcgac gccggtgacg ccggcgaggt cgacgtgggc
      961 ggagcccggc cagtagagac ggtgaccgcc agtgccccac tcacccggcg agtagtggtt
     1021 cgaggtcatc gcccgagacg cggagaaggc aatccgcacg ttgtcgccga ggagcaccct
     1081 ggcccgcgtc gcgatgtccc ggaacgcggt cctgaaccgg ctcgggtcgt ccccgtagtc
     1141 cgagctcttc acattggcgt cggggaggat gtcgaggatg agccggcgcc caccaccgag
     1201 atcggtgaac ctcttgaagg tggccagcat gttgttcagc cgtttgtcgt gagagcccgc
     1261 caccagtccc ctgaggtcat gggccacgat tcggacgtaa ggagtcgcac caccattcca
     1321 gatgttgccc aactggatgg gcaccccgtt gttgggattg ccggtgttgt tgccaccggt
     1381 ggccagccag tctcggtccc cggtggtgtc gtcgatccag tagctgactc cataggtgtc
     1441 gaagggcgcc cctccgctgg cgtagaccgc cagcgcggtc ttgtactcga ggtgggagcg
     1501 cccaccaaag aaatcaggtg gtgagggcgg cggttcggga gtggtgatcg ggtactggtc
     1561 tccgagtgcg cgcttgagaa aggccgccat ctggccccgt gtgaccgagt cgtcggggca
     1621 gaaccggtcg ttggccggcg gattgcaccc gacggtgatg ccggatgcgg cgatctgctc
     1681 gatcgcattc tcgaaaacgg agccgtcgtc gtcgaagaaa gtgcccgacg agcccggctg
     1741 atcgttgcag ccgatcctct ccggtcccgc ggccagggag tcgaaggaga tcgagttgga
     1801 cttcggcgag gtggcgtcgc ccccgtagta cacgtcgaac cagagcgact tcaccctgag
     1861 atgggactcg gcgggtctcc ggaaggtcag attcgtcgca ttgaacgcct ggttcccgtc
     1921 gacatagcct tcgaggatcc cgttgctctc acccagggcg ttcatcttca cccggccttc
     1981 gatgcagtac cacttgccgt gctgcagggt ggagagattg ggatcccagt tgaggatctc
     2041 accactcttg ccggcgcctc ccgagctgag gagatacgcg taccaaccga cccgggtcac
     2101 cctcgccggg tcgtagggga tcgtggggag accgtcaccg ttgtagatgg gcgagaacga
     2161 catccgtgcc gaccagcacg gtgctcccga ggtcgagggc cggttcccgc ggcagttgcc
     2221 ggaatagaga ccggcgaaac cggggagctt gccccgcgac ggcgggtcga cccggaaccc
     2281 gaccgggaac ctgacccaat agcgcatcca catctcctgt ggatcggagc tgagacgcga
     2341 gctggtgttc caatgaccgg tcgatcccca gtgctggccg gcagggatgt tggaaacggc
     2401 gccgtcgccc acatgaccat ccgaagtcgg gtggagactc cagcccgagt agccgcctac
     2461 cccaccttcg aagtcctcgt cgagccaggg gtccgccgcc tgcgccgtct gggggagcac
     2521 gggcagaagc gagaacagca gcgtggccgc gagcagggca agggtgcggg gattggcttt
     2581 cattggagat cgacgcgccc acagcggata gctatccaaa gccggagaca cattcgagtt
     2641 ctcggccgga agtgtcactc actcaagccg ggtggcgcgc ctatgccaaa cagcgggccg
     2701 cttgtcaccc gcgacgggcc cgagccgcct ccaatgtgtt ggagaggagc atcgccctgg
     2761 tcatgggccc caccccaccg ggaaccgggg tgatcgaccc ggccacctgc cgaaccgatt
     2821 cgaagctcac atccccgacc agaccatgct cggtgcgact gatgccgacg tcgatcactg
     2881 ccgcacccgg cttgacgaaa tccgccgtga ccaggccggg gaccccggcg gccgccacca
     2941 ggatgtccgc cgcccgcgcc acctcggcca ggtctgtggt ccgcgagtga gccatggtca
     3001 ccgtcgcatc gacacccttg ctcgccagca tcagagccag agggcgaccc accaggtacg
     3061 accgtccgat gacaaccgcg gtcttgcccg agacggatag cccatgatgc tcgaggatcc
     3121 gcatgatccc gctcggggtg cagggcctga gacctggccg gtcgagcacc aggagcccca
     3181 ggttgtgcgg atgcaaaccg tcgacgtcct tcgccgggga gatccgctcc acgatccgct
     3241 ccgagtcgag atcacccggc agtggcagct gaaccaggat cccgtccacc gagtcgtcgc
     3301 ggttgagtgt gtctatcagc tcctccaatt ctccctgaga gacgtctggg cccagctcgt
     3361 ggtgaaagga cacgttgccg acctccccgg catagcttcg cttgccacta acataagtgt
     3421 gcgaagcggg atc
//
LOCUS       DATITW010000413         1572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_460895, whole genome shotgun sequence.
ACCESSION   DATITW010000413 DATITW010000000
VERSION     DATITW010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460895"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VLS86_05820"
     CDS             complement(<1..208)
                     /locus_tag="VLS86_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50044.1"
                     /translation="MARLGALFVISGADAVGSLAAGVVRVGREVATSADGERMRQAIG
                     AGIAGNNGDALWRALRIDDWLSQMP"
     gene            complement(280..963)
                     /locus_tag="VLS86_05825"
     CDS             complement(280..963)
                     /locus_tag="VLS86_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50045.1"
                     /translation="MRVTNEPDDAGGVLAQLFKLQTEFSARMTEETIRYLRRVQGMSA
                     PAAPETVVLPTGDNTITGSAQPGGTASLELELENRQRVHTVVSTTLTAMVAASGVTWF
                     PRTEIAPSMLLVAPDETRSLVITVPIPPEIPVGKYRGAILMPGFTNGGIPVELSITDR
                     GTKKGTTRSRTRTNTTTSTTRSKTNARSTQPPAKPKTSTAGVTRSKKVGGSDSGRGSE
                     KSKKAGGEQ"
     gene            complement(973..>1572)
                     /locus_tag="VLS86_05830"
     CDS             complement(973..>1572)
                     /locus_tag="VLS86_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSO50046.1"
                     /translation="ESSEGQAIELDWIAARRYDLEDEDYVRMAYKKTCWYTVIAPLRI
                     GVICGTRPGPDAPVGDELLPLTEFGFNAGIAFQIHDDLLNLEADEVLYGKEISGDLWE
                     GKRTIMLLHFLRTAGSAERDRAIEILDAPRARKRPDDVGWLLQAMVANGSLEYGRARA
                     HEFSERAIKIYSRGLPLLTEGDDKRFLREMLRYVIERPK"
BASE COUNT          269 a          527 c          459 g          317 t
ORIGIN      
        1 ccggcatttg agagagccag tcatctattc gaagtgctcg ccacaaggcg tcgccgttgt
       61 tgcccgcgat cccggccccg atcgcctgac gcatcctctc cccgtccgct gaggtcgcca
      121 cctcccgccc cacccggacc actcccgcag ccagtgagcc gacagcatcg gccccggaga
      181 tgacgaacag ggcgcccaat cgtgccacca gagccgcgat caaactcgaa gtcgtccggg
      241 gcggaccatc gacagagtcg accagacgaa ccgacgtggt cactgctcac ctccggcttt
      301 cttggacttt tcggagccgc gtccgctgtc actacctccg accttctttg atctggtgac
      361 cccggcggtg gacgttttcg gcttcgccgg tggctgtgtg ctgcgagcat tcgtcttcga
      421 ccgggtggta cttgtcgtgg tgttcgttct cgttcgcgac cgggtggtcc ctttcttcgt
      481 cccgcgatcg gtgatcgaca actccacggg aattccaccg ttggtgaacc ccggcatgag
      541 gatcgcaccc cggtacttgc ccacaggaat ctctggcggt atcgggacgg tgatgaccag
      601 tgaccgtgtc tcatcagggg caaccagcaa catcgacggt gcgatctcgg tccgcgggaa
      661 ccacgtcacc ccggacgccg cgaccatagc ggtcaacgtg gtcgagacca ctgtatgaac
      721 acgctgtcgg ttctccagct cgagctccag gctcgccgtc ccccctggtt gtgccgagcc
      781 ggtgatggtg ttgtcaccgg tgggcaatac gacggtctcg ggcgcggcgg gcgccgacat
      841 cccctgtacg cggcgtaggt aacggatcgt ctcctcggtc atccgggccg agaactcggt
      901 ctggagcttg aagagctgtg ccagcacccc accggcatcg tcgggttcgt tcgtcacgcg
      961 catctgtccc tcctacttgg gccgctcgat cacataacgg agcatttccc gcagaaaccg
     1021 cttgtcatca ccctctgtga gtagcggcaa cccgcgcgag tagatcttga tggctcgctc
     1081 cgagaactcg tgtgcccggg cgcgcccgta ctcgagtgac ccgttcgcga ccatagcctg
     1141 gagcagccag cccacatcat caggcctctt tcgggcgcgg ggggcatcca ggatctcaat
     1201 ggctcggtcg cgctcggccg agcccgctgt gcgtaggaag tgaaggagca tgatggtgcg
     1261 ctttccctcc cacagatcgc ccgagatctc cttcccgtag agcacctcat cagcctcgag
     1321 attcaatagg tcatcgtgga tctgaaaagc gatgccggcg ttgaacccga actcggtcaa
     1381 aggaagcagt tcgtcaccaa cgggggcatc cggtccgggc ctggtcccac agatcacgcc
     1441 gattcgaagc ggtgcgatga ccgtgtacca gcacgtcttc ttgtaggcca ttcgtacgta
     1501 atcctcgtct tcaaggtcgt atcttctggc ggcgatccag tcgagctcga tcgcctgacc
     1561 ttccgacgac tc
//
LOCUS       DATITW010000414         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_340574, whole genome shotgun sequence.
ACCESSION   DATITW010000414 DATITW010000000
VERSION     DATITW010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340574"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..967)
                     /locus_tag="VLS86_05835"
     CDS             complement(95..967)
                     /locus_tag="VLS86_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSO50047.1"
                     /translation="MTPIALDHVVAIDFHVHVELGPDGDDHLSPALRQAVSRYFRGDG
                     NLPTIDEVAEHYRSRHMLAVVFGVDSALTTGQPRIPNQFIVQKSLEHPDVLVPFASID
                     PRRGPEGLAEARDLIASGIVRGFKFHPNLQQFEPNDGSAYPLFEVIEGGALSAMFHTG
                     HSGIGAGLPGGGGIRLKYGNPMSIDDVAVDFPGMKIVMAHPSFPWQDEALSVAMHKPQ
                     VLMDLSGWSPKRFPPQLVPAISASLREQTLFGSDFPLITPDKWLRDFSTLEIDEATSA
                     LILKENAARLLGLV"
     gene            1023..>2119
                     /locus_tag="VLS86_05840"
     CDS             1023..>2119
                     /locus_tag="VLS86_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSO50048.1"
                     /translation="MQDIQLSIPLILRRAATIGASLGVTSVEPAGIDHRSWPQIADRA
                     MRVAAALDVLGVEKGKAIGSFAWNGHRHLELYYGVPCSGRVLHTINVRLHLDVIEYVI
                     AHADDQVIFVDASLTPVLAPLRDRLKAQVVLMEDGAAVDPVFDRDPRYEELLASVEPT
                     EPFEIEEDQAASICFTSGTTGRPKAVVYSHRSVVLHSMAELMVDGHAVRRGDVILPLT
                     PMFHVNCWGLPYAAGLAPASLVFAGADTSPRAAARLIERSKPTVLAGIPTFWVQMDHV
                     FASGEYDLSSVKRILCGGAEAPPALIEKYTNRGIDFFHAWGMTEMSPSGTGNWIPARS
                     MEIVANHGVAAPLVDLRLVDRDGGELAWDGV"
BASE COUNT          389 a          639 c          681 g          410 t
ORIGIN      
        1 gacaggcttc ctgggcatgt tccctcgtct cctcgagaag gctcgaaatc gctggctgtt
       61 cggtatgcga accactgttc gggttgcgga cacgttacac gagacccaac aaccgggccg
      121 cgttttcctt gaggatcaga gcggaggttg cttcgtcgat ttccaatgtg gaaaaatccc
      181 tcagccattt gtcaggggtt atcaagggga agtccgaccc gaagagagtt tgctctctca
      241 gcgaagccga gatcgctggg accaattggg gcgggaagcg ctttgggctc caccccgaga
      301 gatccatgag cacctggggc ttgtgcattg ccacagagag ggcttcgtcc tgccacggga
      361 acgagggatg ggccatgacg atcttcatac cggggaaatc aactgcgacg tcgtcgatcg
      421 acatcgggtt gccgtacttg agtctgatcc ctccgccacc ggggagtccc gccccgatcc
      481 cgctgtgccc ggtgtggaac atggcactca acgcgccccc ctcgatcacc tcgaacaagg
      541 ggtaagccga cccgtcgttg ggctcgaact gctgaaggtt gggatggaac ttgaagcccc
      601 ggacgatccc cgaggcgatc aggtcccgtg cctctgccag gccctcgggg ccgcgtctgg
      661 gatcgatgct ggcaaaaggg acgaggacgt caggatgctc gaggctcttc tgcacgatga
      721 actggttcgg gatccttggc tggccagtgg tcagggcgga gtcgacaccg aagacgacgg
      781 cgagcatgtg cctgcttcga tagtgctcgg cgacctcgtc gatcgtgggc aggtttccgt
      841 caccacggaa gtaccggctg accgcttgcc ggagtgcggg cgagagatgg tcgtcgccgt
      901 cgggccccag ctcgacatga acatggaagt cgatagctac cacgtggtcg agcgcaatgg
      961 gggtcacgga tatatattcg agcctagccc cgagaccagg aggaacgcgt gtgagagggc
     1021 tgatgcagga catccagctc tccatccccc tcatcctccg acgggccgca accattggtg
     1081 cctcactggg tgtgacctcg gtcgaacccg cggggatcga ccatcgcagc tggccgcaga
     1141 tcgccgaccg ggcaatgcga gtggccgccg cactcgatgt cctcggagtc gagaagggga
     1201 aagccattgg cagcttcgct tggaacggcc accgacatct cgagctgtac tacggagtgc
     1261 cttgctcggg gagggtcctc cacacaatca acgtgaggct ccaccttgac gtcatcgagt
     1321 acgtgattgc acacgccgac gatcaggtga ttttcgtaga cgcctcactt acgccggtgc
     1381 tggcgccact ccgtgaccgc ctcaaggctc aggtggtgtt gatggaggac ggtgctgccg
     1441 tcgacccggt cttcgatcgg gacccgcgat acgaggagtt gctcgcctcg gtggagccaa
     1501 cagagccgtt cgagattgag gaggaccagg ccgcatccat atgcttcaca agcgggacta
     1561 ccggccgtcc caaggcggtg gtttactccc atcgctcggt tgtactccac tccatggcgg
     1621 aactcatggt cgacggtcat gccgttcgtc gcggagacgt gatcctgccg ttgaccccga
     1681 tgtttcacgt caactgctgg ggtcttccct acgcggccgg cctggcacct gcctcacttg
     1741 tctttgcagg agccgacacc agcccacgag cggcggcccg tctgatcgag cgttcgaagc
     1801 cgaccgtcct cgccgggatc ccgacgttct gggtgcagat ggatcacgtc ttcgcctcag
     1861 gcgagtacga cctgtcttcg gtgaagcgga ttttatgcgg gggggcggag gcccctcccg
     1921 ctctcatcga gaagtacacc aatcgaggta tcgacttctt ccacgcgtgg ggcatgaccg
     1981 agatgtctcc cagcgggacc ggtaattgga taccagcccg cagtatggag atcgtggcga
     2041 accacggggt ggccgccccg ctggtcgacc tccggctggt cgaccgagac ggtggtgagt
     2101 tggcctggga cggggttag
//
LOCUS       DATITW010000415         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_781258, whole genome shotgun sequence.
ACCESSION   DATITW010000415 DATITW010000000
VERSION     DATITW010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781258"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VLS86_05845"
     CDS             complement(<1..643)
                     /locus_tag="VLS86_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382189.1"
                     /note="with PdxT forms pyridoxal 5'-phosphate from
                     glutamine, either ribose 5-phosphate or ribulose
                     5-phosphate, and either glyceraldehyde 3-phosphate or
                     dihydroxyacetone phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HSO50049.1"
                     /translation="MEFAREHTVETGTDRVKRGLAEMLKGGVIMDVVTADEAKIAEEA
                     GAVAVMALERVPSDIRKHGGVARMADPTKIEEIQASVSIPVMAKCRIGHFAEAAVLQA
                     LGVDYIDESEVLTPADEEHHVDKWAFTTPFVCGARNLGEALRRIGEGAAMIRTKGEAG
                     TGNVVEAVRHIRTVTNEIRALTTMDQAQLMAAARDHAAPYDLVREVASTGALPV"
     gene            complement(668..1702)
                     /locus_tag="VLS86_05850"
     CDS             complement(668..1702)
                     /locus_tag="VLS86_05850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSO50050.1"
                     /translation="MSRIRVGIVSPYDLSEPGGVQAQVLGLARYLDAAGDRALIIGPG
                     LPQGTDGVDLGRSVSIPGNGSMVPLSIDPRVRGRIRAAVADVDLLHVHVPLMPLVSVF
                     ANHSGPPVVATFHAAPGRVGRLAYRLMGPLFGWALGNAVAVTAVSEAAAAAIPPGREV
                     RIIPNAIDVSSLTVDTPRIEQRVAFLGRDEPRKGLEVLLEAWRTVADTHPAAQLVVMG
                     ADRGTPGVTWMGRVDDEAKARVLNSASVFVAPQLGGESFGIVILEAMAAGAAVVASDL
                     EPFQALAGGGARFFPVGDSAALAAVLGDLLDDEGEKSRLGAAGRAGAAGYDWSVVGAQ
                     YRELYASNLS"
     gene            complement(1699..>2240)
                     /locus_tag="VLS86_05855"
     CDS             complement(1699..>2240)
                     /locus_tag="VLS86_05855"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HSO50051.1"
                     /translation="YALPHVGNWESAAPIAVAEGVPVVAVAEKLANHRITDWFTAMRA
                     EFGIEIVLATGATEVMRKLEAALNANKAVALLSDRDLKQRGVEVEFFGEKTTLPPGPA
                     TLALRAGAPLFPVATYFDGNDGYRVVVKPAIPVPVEGSRTEKVTAMTQALAKELEKLI
                     LAAPDQWHLVQPNWPSDRE"
BASE COUNT          356 a          820 c          705 g          359 t
ORIGIN      
        1 ccacgggtag ggccccggtg gacgccacct cgcgcaccag gtcgtaggga gcggcgtggt
       61 cacgggcggc ggccatcagc tgggcttggt ccatcgttgt cagggcgcgg atctcgttgg
      121 tgacggtgcg tatgtggcgc acagcctcga cgacgttgcc ggtgccggcc tcacccttgg
      181 tgcggatcat ggccgccccc tccccgatcc gtcgcagggc ctcacccagg ttgcgggcac
      241 cgcacacgaa tggtgtggtg aacgcccatt tgtccacgtg atgctcctcg tcggccgggg
      301 tcaagacctc ggactcgtcg atgtagtcga cgcccagcgc ctggaggacc gctgcttcgg
      361 cgaaatggcc gatgcggcat ttggccatga ccgggatcga caccgaagcc tggatctcct
      421 cgatcttggt cgggtcggcc atgcgggcca cgccaccgtg cttgcgaatg tcggagggga
      481 cccgctccaa ggccatcacc gcgacggctc cggcctcctc ggcgatcttc gcctcgtccg
      541 cggtgacgac atccatgatg acgccaccct tgagcatctc ggcgaggcct ctcttcacac
      601 gatctgtacc ggtttccact gtgtgctccc ttgcgaactc caatgataca gggtcctacg
      661 ccctgaccta agagaggttc gaggcgtaca gctcccgata ttgggcgccc accacactcc
      721 agtcatagcc ggccgcgccc gctctgccgg cggcgccgag ccgggacttt tccccttcgt
      781 cgtcgagcag gtcgccgaga accgcggcca gggcagcgct gtcgccaacc gggaagaacc
      841 gcgctccgcc accggcgagg gcctggaagg gctccagatc cgatgccacc accgcggccc
      901 cggccgccat cgcctcgagg atcacgatcc cgaaggactc cccgcccagt tgtggcgcca
      961 cgaacaccga ggccgagttg aggacgcggg ccttggcctc gtcgtcgacc ctccccatcc
     1021 aggtgacacc cggcgtcccc cggtcggcgc ccatcacgac cagctgagcc gcggggtggg
     1081 tgtcggccac cgtccgccac gcctcgagca gcacttccag cccttttctc ggctcgtccc
     1141 tgcccagaaa ggcaacccgc tgttcgatcc tcggggtgtc gactgtcagc gacgagacgt
     1201 ctatcgcatt ggggatgatc cgcacctctc gtcccggggg aatcgccgcg gcggcggcct
     1261 cactgacggc ggtgaccgct accgcgtttc ccagcgccca accgaacagc ggccccatca
     1321 gccgataggc gagacgaccc acccgacccg gtgccgcgtg gaaagtggca accaccggcg
     1381 gcccgctgtg gttggcgaac accgagacca ggggcatcaa cggcacgtgg acgtggagca
     1441 ggtcgacgtc agcgaccgcg gcccgaatcc tccctctcac cctggggtcg atggagagcg
     1501 ggaccatcga tccattgccc gggatcgaga cactgcgccc caggtcgacg ccatcggtgc
     1561 cctggggaag cccgggaccg atgatcagcg cccggtcgcc ggcagcgtcg aggtagcggg
     1621 ccagaccgag gacctgtgcc tggaccccgc ccggctccga gaggtcatag gggctgacaa
     1681 tcccgacccg gatccggctc actccctatc cgagggccaa ttcggctgca ccagatgcca
     1741 ctggtcggga gcggcaagga tcagtttctc caactccttg gccagggcct gggtcatcgc
     1801 cgtcaccttc tccgtccgcg acccctcgac cgggaccggg atcgccggtt tgaccaccac
     1861 ccgatacccg tcgttgccgt cgaagtaggt ggcgaccgga aacaacgggg ccccggcgcg
     1921 aagtgccaga gtcgccggcc ccgggggcaa tgtcgtcttc tccccgaaga actcgacctc
     1981 gacgcctcgt tgcttcaggt cgcggtccga gagcagcgct accgccttgt tggcgttcag
     2041 cgccgcctcc agcttcctca tcacctccgt ggcccccgta gccagcacga tttcgatccc
     2101 gaactcggcc cgcatcgcgg tgaaccagtc ggtgatccgg tggttggcca gcttctctgc
     2161 caccgccacc accggaaccc cctcggccac cgcgatcggg gcggccgact cccagttgcc
     2221 gacatggggc agcgcataga
//
LOCUS       DATITW010000416         1729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_460974, whole genome shotgun sequence.
ACCESSION   DATITW010000416 DATITW010000000
VERSION     DATITW010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460974"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VLS86_05860"
     CDS             <1..223
                     /locus_tag="VLS86_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HSO50052.1"
                     /translation="EGSPDHVVRHGIDARGRLPTDLGVKDTVLVWPGETVRIAVDLRH
                     PFDAEQHLLYHCHILEHEDAGMMADLLIV"
     gene            246..689
                     /locus_tag="VLS86_05865"
     CDS             246..689
                     /locus_tag="VLS86_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSO50053.1"
                     /translation="MTDNVVADDAPKVLQLRLVVTAEDLDAALAFYRDTLGLTERLAA
                     DGPDGARVVILDAGQATLELINSAQRRYIDDVEVGRQVSRQVRVAFEVEDSAATTEVL
                     TEAGAELLAAPVETPWRTLNSRLEAPAGLQITIFQELDPPEAVPG"
     gene            1136..1315
                     /locus_tag="VLS86_05870"
     CDS             1136..1315
                     /locus_tag="VLS86_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50054.1"
                     /translation="MDKPMTLIMNDGSSQTVNVSDTDLEALEWWISNVRKPNPIYIEG
                     VLYLRETVKELVATG"
     gene            1470..>1729
                     /locus_tag="VLS86_05875"
     CDS             1470..>1729
                     /locus_tag="VLS86_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50055.1"
                     /translation="MSQEHKDALAQGRLEARSIKAYLRALESRRPGRPVTKESLDKRL
                     DRVNTKLGSESDPLKRIDLLQSKLDLESSLANTKEGANLDELS"
BASE COUNT          351 a          493 c          564 g          321 t
ORIGIN      
        1 ggaggggagc cccgatcatg tcgtgaggca cggaatcgac gcccggggcc gccttcccac
       61 cgacctgggt gtcaaagata cggtgctcgt gtggccgggc gagacggtga ggatcgccgt
      121 cgacctgcgg catcccttcg acgccgaaca gcatcttctc tatcactgtc acatcctcga
      181 acacgaggac gcaggcatga tggctgacct gctcatcgtc tagttggccc gctacgttgc
      241 ggccgatgac agacaacgtc gtggccgatg atgcgcccaa ggtgctccag ctcaggttgg
      301 tggtgaccgc ggaagacctc gacgccgcgc tcgccttcta ccgcgacacc ctcggactga
      361 ccgaacggct cgccgcggac ggcccggatg gggccagggt ggtgatcctc gacgcgggac
      421 aggccaccct ggagctgatc aacagcgctc agcgtcgcta catcgacgac gtcgaggtcg
      481 ggaggcaggt gagccgtcag gtcagggttg ccttcgaggt ggaggactcg gcggcgacca
      541 ccgaggtgct cactgaagcc ggtgccgagc tgctcgcagc cccggtagag acaccctggc
      601 gcactctcaa ctccagattg gaggctccgg ccggtctcca gatcacgatc ttccaggagc
      661 tggaccctcc cgaggctgtc ccgggatgac cggtccgtga tcagccggtc gaagcccggg
      721 aaaagaggag ggcgttcgcc ggggtcgaaa gagtgctttg tggcgtaggg tggagctgtg
      781 agcgacctga gacgggatca ctggtaccgg gtgagctact tcatggccgg acaggagaga
      841 gaggctgtag gcaaatacct ggggctttcg acgtggaatg acctgcccga gcacaaacat
      901 ggagagccga ctcctcaggg cagtggcgac gtcgtatatc actggttatc gattcgtgat
      961 cagaattggc cgttgagcat tcgacctgac gatgtgatcc gcgtcttgga gatcggcgaa
     1021 ccgcccggtc ggtctcgctt gtcgaggatt acctctcgct gaccggcagc ttcttttctc
     1081 tcggttgaag aagcattgcc gtggttgtgg agggtgccgg tgccagcatg accagatgga
     1141 caagccgatg accctgatca tgaatgatgg ctcttcgcag acggtcaacg tctccgatac
     1201 ggatctcgag gcgctggagt ggtggatctc caacgtgaga aagccaaacc cgatctatat
     1261 cgaaggcgtt ctctacctac gggagaccgt caaggaactg gtggccaccg gctgattcgg
     1321 ggagcggcgc cgggagcggc gcgcgattga gtcacgaatg ctgagcactg cgcgctgttt
     1381 tcatcggtca atccggatta ccgattgtcc tggcatatgc catcatcacg aagtcgaatt
     1441 ccgcacggat tggaggatgg acagtgccga tgtcacagga acacaaggat gccctggccc
     1501 aggggagact cgaagctcgc tcgatcaagg cctatcttcg ggctttggag tcacgtcgac
     1561 cggggcgacc ggtgaccaag gagagtctgg ataaacggct ggatcgagtc aacaccaaac
     1621 tcggatccga gtctgaccct ctgaaaagga tcgatctgct gcagtcgaag ctggacctcg
     1681 aaagctccct ggccaacacc aaagagggag cgaatctcga tgagttgtc
//
LOCUS       DATITW010000417         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_621450, whole genome shotgun sequence.
ACCESSION   DATITW010000417 DATITW010000000
VERSION     DATITW010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621450"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..968)
                     /locus_tag="VLS86_05880"
     CDS             complement(78..968)
                     /locus_tag="VLS86_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50056.1"
                     /translation="MSDLAVRDKPLEYWFFWFNAGSLAFLVDYVIRRSLRIAEVRVSF
                     WVDGLGRVERLSSPTWLASGARVDIAECTFDEQSSTGRVADVEWDLRLELGSSRIDPA
                     VPPIKWLNPFDMQIASRPRARFSGQVTVGDVTFSCEDVPGLVSHYWGRRLPDSWWWIS
                     ANLFEDTDLAVEALLARTRVWNSARVAMNAGYLWIEDQGRRQLVIHPLNGLIRIEGTP
                     ESFTLRARQLGRPGFTLRCSASPHSYNDLGEDIQQTLLGTCVIQGMGEARGTAGLEIR
                     SRATAPTTNWHPDDLGEQAV"
     gene            1465..1881
                     /locus_tag="VLS86_05885"
     CDS             1465..1881
                     /locus_tag="VLS86_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSO50057.1"
                     /translation="MTNHEHPNVTLVREGFAAFRSGDMAWMDAHLADDVVWHVGGNSK
                     WAGAYQGKDKVLDLFARQAQAMGGQPPAPEIHDILGNDEHVVALGEASASAADGSSAE
                     WKYVQVFHVKDGMATEVWGMAENDAAVDPFLDGLPG"
BASE COUNT          406 a          639 c          654 g          377 t
ORIGIN      
        1 accgtgcgcc tccgactcat gggcccatgc acgtcccgtt tctaaagggc ccgagacgtc
       61 gccgcccgtt ccgcttctca gaccgcttgc tcgccaagat catcagggtg ccagttggtg
      121 gttggtgcgg ttgctcggga acggatttcg agcccagcgg tgccacgcgc ctctcccatt
      181 ccttggatga cgcaggttcc gaggagggtc tgttggatgt cctcgccaag atcgttatag
      241 gaatgcggtg acgcgctgca gcgcagggtg aagcctgggc gacccagttg ccgagctctt
      301 agagtgaagc tctcgggcgt tccttcgatg cggatcagcc cgttgagggg gtgaatcacc
      361 agttggcgac ggccttggtc ttcgatccag aggtatccag cattcatggc aacgcgagcc
      421 gagttccaga cccgcgttcg ggcgagcagc gcctcgacgg caagatcggt gtcctcgaag
      481 agattcgccg agatccacca ccagctgtcg ggtagccgtc ttccccaata gtggctcacc
      541 aagccgggaa cgtcttcaca cgaaaatgtg acatcgccca ccgtcacctg accagagaac
      601 cttgctcggg gtcgactggc gatttgcatg tcgaagggat tcagccactt gataggtggc
      661 accgccggat cgatgcggct gctacccagt tccaggcgga ggtcccactc gacatccgcc
      721 actcgacccg tcgagctctg ctcatcaaag gtgcattctg cgatgtccac cctcgccccg
      781 cttgcgagcc aagtcggtga gctcagcctc tcgactctgc ccagcccatc cacccaaaac
      841 gaaacccgca cctcggcgat tctgaggctg cggcggatga cgtagtccac gagaaaggcc
      901 agggagccgg cgttgaacca gaagaaccaa tactcgagcg gtttgtcccg caccgccaga
      961 tcgctcatcg gacaagacga tacctggacc cgactagcag gcccgtggga cgcgggggcg
     1021 gccgaccatg gttgggattc caaatccgat cctcagacgg cccggttcag gggcgaatga
     1081 cgatgctgtc gaggatcgcc cggagttgag cgacatcctc cgatttggcg ttcgacagct
     1141 tgttcattcg gccagacacg tcccgtttct aaattcctgc cgtgtgacgg tgagtgcaca
     1201 gccggtgaaa gaggtgggtg acccgcttcc cggcggccga cggtgatcac catctccatt
     1261 tccatcgcgg ggtccaaggc ggtatcccca gcttcaccga gggcctcgat acccgggcat
     1321 ctggcccggc ggcgccggga ccggggagag taggctcaat ggggtaggtc gttcgacgac
     1381 aggcgggccg ttgcccgcgg gcccgaacag ctggaaaacc agagggcatg gatcagagac
     1441 gcccaggacc aaaggaggcc agacatgaca aatcacgagc atcccaacgt cactttggtt
     1501 agggaaggct tcgcggcctt ccgtagtgga gacatggcgt ggatggacgc ccatctcgcc
     1561 gacgatgttg tctggcacgt cggggggaac agcaaatggg caggggctta ccagggcaag
     1621 gacaaggtcc tcgacctatt cgcccgtcag gctcaggcca tgggcggaca accaccagcg
     1681 cctgagatac acgacatctt ggggaacgac gagcacgtgg tcgcactcgg ggaggcatcg
     1741 gcgtcggcgg ctgacggttc cagcgccgag tggaaatatg tccaggtctt tcacgtgaag
     1801 gatggcatgg ccaccgaggt ctggggaatg gcggagaatg acgccgccgt ggacccgttc
     1861 ctggatggtc tccccggcta gagcgcccaa aacatccgct caatcggtga gcccgccaag
     1921 acggagaatt tggcgctggc tccgtgaaca tcgaagagat catcggacag gccggggacg
     1981 ccattcgctt cgccgtcccc aactagtccc gtttctcagg cgcctctggt ttgtgggggc
     2041 ggccatcact cgcatcgggt gacgtactcc tcgaac
//
LOCUS       DATITW010000418         1518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_300757, whole genome shotgun sequence.
ACCESSION   DATITW010000418 DATITW010000000
VERSION     DATITW010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300757"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VLS86_05890"
     CDS             complement(<1..216)
                     /locus_tag="VLS86_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO50058.1"
                     /translation="MLEVRGLTHSFGGLRAVDGVDFEVQRGEVVGLIGPNGAGKTTVF
                     NLISGAIRARQGSIHFREEEITGLRPDQ"
     gene            complement(218..1213)
                     /locus_tag="VLS86_05895"
     CDS             complement(218..1213)
                     /locus_tag="VLS86_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSO50059.1"
                     /translation="MLISTIRRHEKSALVAIPVTIAALAVAPFVVPESVAITLITVLM
                     YSTLTLAWAMFSGPTRYISLATAGLFGIGVYVTAGLRGRLPLEVIVLIGGGAALIVAL
                     AIGLLTLRLKGVYFILFTFGITALIRNSIQWWETHVAGTVGRHVSGATNEVVYLLLLI
                     LFSVTLIAAFLLRHSGPGLALEAIGENQDAAEHIGINVTQLKVSTFATSSLLIGATGA
                     VMATRFRYIDTNVAFNPLISFLPVLMAILGGTSKLYGPVLGSVILVVLQEFLVTEYPY
                     VYLLLFGLVLIAVVLWLPGGLVVLAQRSYFRLKIRWIKLRMRISRFLRIRQARRM"
     gene            complement(1203..>1518)
                     /locus_tag="VLS86_05900"
     CDS             complement(1203..>1518)
                     /locus_tag="VLS86_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSO50060.1"
                     /translation="RVVLALCFGLGAALAGLAGVLYSLMFEIEASRGIELTVVAIVVV
                     EEGVLGSITGSLLGGFVLGLVIAVVNYFDPSLSLVAFYLIFSVVLLVKPSGLLGRRIH
                     AD"
BASE COUNT          340 a          478 c          468 g          232 t
ORIGIN      
        1 ctggtcgggt cgaagcccgg tgatctcttc ttctcggaaa tgtatcgagc cctgtcttgc
       61 ccggatcgca cccgagatca gattgaaaac ggtggtcttc ccggccccgt tggggccgat
      121 gagcccaacc acctcaccac gctgaacttc gaagtcgaca ccgtcgaccg ctctcagacc
      181 cccgaagctg tgggtgagac ccctgacttc gagcacctca catcctccga gcctgtcgga
      241 ttcggaggaa ccggctgatc cgcatccgca acttgatcca ccggatcttg agccggaagt
      301 aggagcgctg ggcgagcacc acgagcccac cgggcagcca caggaccacc gcgatcagga
      361 ccaggccgaa caagagcaga tacacgtacg ggtactcggt gacgaggaat tcctggagga
      421 cgaccaggat caccgacccc agcacgggcc cgtagagctt ggaggtgccc ccgagtatcg
      481 ccatcagcac cggaaggaat gagatcagcg ggttgaacgc gacattagtg tcgatgtagc
      541 ggaagcgggt ggccatcacc gcaccggttg ccccgatcag cagggaactg gtggcgaagg
      601 tcgacacctt gagctgtgtc acgttgatcc cgatgtgctc ggcggcgtct tggttctccc
      661 cgatcgcctc cagggcgaga cccgggccgg agtgacgaag cagaaacgcc gcgatcaggg
      721 tgacggagaa caggatcagt aacaggaggt agacgacctc attggtcgcc ccgctgacgt
      781 gtctcccgac cgttcccgcc acatgcgtct cccaccattg gatcgagttc cggatcagtg
      841 cggtgatgcc gaaggtgaag aggatgaagt acacaccctt caggcgcagg gtgagaaggc
      901 caatggcgag ggcaacgatc agggcggccc cgccaccgat gagcacgatc acctcgaggg
      961 gaagacgccc ccgcagaccc gcggtcacgt agacaccgat tccgaacagt ccggcagtcg
     1021 ccagcgagat ataccgggtg ggccctgaga acattgccca ggccagtgtg agtgtggagt
     1081 acatcagcac cgtgatgagg gtgatcgcca cggactcggg aaccacgaag ggggcaacgg
     1141 ccagtgcggc gatggtcacc ggtattgcca ccagagccga tttctcgtga cgacggatgg
     1201 tgctaatcag catggatccg ccttcccagc aggccagacg gtttgaccag cagcaccacg
     1261 ctgaagatga ggtagaaggc gaccagtgag aggctgggat cgaagtagtt caccaccgcg
     1321 atcaccagac ccaggacgaa accgccgaga aggctcccgg tgatgctgcc aagcactcct
     1381 tcctccacca ccacgatggc caccacggtg agctcgatcc cgcggctcgc ctcgatctcg
     1441 aacatcaagc tgtagagcac acctgccagg cctgcgagag cggctcccag gccaaagcac
     1501 agagccagca ccacgcgt
//
LOCUS       DATITW010000419         1729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_661111, whole genome shotgun sequence.
ACCESSION   DATITW010000419 DATITW010000000
VERSION     DATITW010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661111"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VLS86_05905"
     CDS             <1..544
                     /locus_tag="VLS86_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO50061.1"
                     /translation="CEVCNGRRYNRETLEILWTGHNIADVLEMSVEEALGFFENQPKI
                     VRILQTLYDVGLGYVRLGQPATTLSGGEAQRIKLASELGKRATGRTFYILDEPTTGLH
                     FEDIRKLLQVLQRLVDAGNTVVVIEHNLDVIKSVDYVIDLGPEGGSGGGRIIAQGTPE
                     EIAQVGESYTGKFLREMVGG"
     gene            571..>1729
                     /locus_tag="VLS86_05910"
     CDS             571..>1729
                     /locus_tag="VLS86_05910"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HSO50062.1"
                     /translation="MFFNSLAFAVFLPTVVGLYWIVPARHRTVLLLLASYVFYGWWDV
                     RFLSLIVISTLVDFMVGRRLGITEDPRHRKRWLLVSVIANLGMLGFFKYWGFFVDSAA
                     TLLSSFGLEPNLPLLQVVLPVGISFYTFQTLSYTIDVYRRHLEPEASLTRFALFVAFF
                     PQLVAGPIERARHLLPQLRNLPSTPRQIDWSGSSKLIVRGLFRKVVIADGLAPLVNEV
                     FATPGRYGSITVALGVIAFSLQIYGDFAGYTDIARGTARLFGVDLMENFKAPYLSRGF
                     SEFWRRWHISLSTWLRDYLYLPLGGSRGGRLATSRNVMLTMLLGGLWHGAAWGFVVWG
                     GLHGVYLVVERWVLRDRTKDGDKSRGLPVILVFAIVTLTWIPFRAGTLSAAG"
BASE COUNT          282 a          539 c          550 g          358 t
ORIGIN      
        1 gtgtgaggtg tgcaacgggc gccgttacaa ccgggaaacc ctcgagatcc tgtggacggg
       61 gcacaacatc gccgacgtgc tcgagatgtc ggtcgaggag gcgttggggt tcttcgaaaa
      121 ccaacccaag atcgtgcgca tcctgcagac cctgtacgac gtcgggctcg gttatgtgcg
      181 tcttggccag cctgccacca ccctctccgg gggcgaggcg cagcggatca agctcgcctc
      241 cgagctcggg aaacgagcca cagggcggac tttctacatc ctcgacgagc cgacgaccgg
      301 gcttcacttc gaggacatcc gcaaactgct ccaggtgctg cagcggctgg tcgacgccgg
      361 gaacacagtg gtggtgatcg agcacaacct cgatgtgatc aagtcggtcg actacgtgat
      421 cgacctcggt cccgaaggag gaagcggggg agggcgcatc atcgcccagg ggacgccgga
      481 ggaaatagcc caggtggggg agagctacac cggcaagttc ttacgggaaa tggttggggg
      541 ctgagtctga acagccgaat ataactttcg gtgttcttca actcgcttgc cttcgccgtt
      601 ttcctgccga ccgtggtggg gctctactgg atcgtgccgg cgcgtcaccg gacggtcctc
      661 ctcctcctcg cctcctacgt cttctacggc tggtgggacg tgcggttcct gagcctgatc
      721 gtgatctcca ccctcgtcga cttcatggtg ggtcggcgtc tcggtatcac agaggaccca
      781 cgacaccgca agcgctggct cctggtcagc gtcatcgcca atctcgggat gctgggcttc
      841 ttcaagtact gggggttctt cgtcgattcg gcggcgaccc tgctctcttc gttcgggttg
      901 gagccaaacc tccccttgct tcaggtcgtc ctcccggtcg ggatctcgtt ctacaccttc
      961 caaaccctgt cctacacgat cgacgtctat cggagacacc tggaaccgga ggcgagcctc
     1021 acccggttcg cactgttcgt tgccttcttc ccccagctgg tggcgggccc gatcgaacgg
     1081 gctcgacacc tccttcccca gttgcggaac ctgccgagca caccgcgtca gatcgactgg
     1141 tcgggatctt cgaagctgat cgtcaggggt ctgttccgaa aggtggtgat cgccgatggc
     1201 ctggcaccac tggtcaatga ggtcttcgcc actccgggtc gatatgggag catcaccgtc
     1261 gcgctcgggg tgatcgcgtt ctctctccag atctacggtg acttcgcggg gtacaccgac
     1321 atcgcccgcg gcaccgcccg gctgttcggg gtcgacctca tggagaactt caaggcaccg
     1381 tatctgtcgc gtggcttcag cgagttctgg cgacggtggc acatctccct gtccacctgg
     1441 ttgcgcgact acctgtacct gccgctaggg ggaagtcggg ggggccggct ggcgacgagt
     1501 cgcaacgtga tgctgaccat gctcctcggc ggtctctggc atggcgccgc ctggggcttt
     1561 gttgtctggg gagggctgca cggcgtctac ctcgtggtcg agcggtgggt gctgagagac
     1621 aggacgaagg acggcgacaa gtcgcgaggt ttgcccgtca ttctggtatt cgccatcgtc
     1681 accctcacct ggataccgtt ccgggctgga accctctccg ccgcagggg
//
LOCUS       DATITW010000420         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_260755, whole genome shotgun sequence.
ACCESSION   DATITW010000420 DATITW010000000
VERSION     DATITW010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260755"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1028)
                     /locus_tag="VLS86_05915"
     CDS             complement(123..1028)
                     /locus_tag="VLS86_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HSO50063.1"
                     /translation="MRELEHLATHSNVMYRVVTNDGRQLVLRVGDPFGNSRANIEYEV
                     AWLSALNRETDIDVVNPIPTGYGALIVDAREPESDLTRPCVLFSWVPGVPLADGAGAF
                     GYRLLGAMSAALQAHGKTWQPDNPEGMRKWDRIFYYDKGLDPIIIYDSRFDHLFSPGR
                     RTIIDKAGQAATNVINASLEVGTTQVVHGDLHEWNVHTVRTRLYAFDFEDVMIALPSQ
                     DISVCLYSSRTNPRAADIQAAYRKGFEEIAPWPIEDQEQLDGFHAARQIMLMNYAART
                     LPMGETEQFLSQVMPWLARYAARYG"
     gene            complement(1170..>1717)
                     /locus_tag="VLS86_05920"
     CDS             complement(1170..>1717)
                     /locus_tag="VLS86_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSO50064.1"
                     /translation="AVPERVLVVVDEAYSDFVTDPSYRTSVSLAMERPNVVVLRTFSK
                     IFALAAHRVGYAVGQPATIAELRKTQLPFSISQAAQTAATFSLGDEAEYRRRVEANAI
                     GRHYLLGVLEERGIFHTESHANFVYLRIGEDSGAVSDDFMRRGVIVRPMSRGWLRVTV
                     GRDRENQRFVSALDEIIAAQP"
BASE COUNT          337 a          528 c          539 g          313 t
ORIGIN      
        1 gacctcaaag ccgataggaa ccccgccccg gggcaacgcg gcaaccaggg ggtcgacagg
       61 ggcgcgttcc accaccgcct gaccgagcag gacgcctgcc tcccggcggt cagcgaatcg
      121 catcagccgt atctggctgc gtatctcgcc aaccagggca tgacctggga caggaactgc
      181 tcggtctcgc ccatcggcag cgtccgcgcc gcgtagttca tcagcatgat ctgtcgtgcg
      241 gcgtgaaagc cgtcgagctg ctcttggtct tcgatcggcc agggagcgat ctcctcgaag
      301 cctttgcgat acgcggcttg gatgtctgca gcccgcgggt tggtgcgtga cgaatagagg
      361 caaacggata tgtcctggga ggggagagcg atcatcacgt cctcgaagtc gaacgcatag
      421 agccgggtcc gcacggtgtg gacattccac tcgtgaaggt cgccatggac gacttgggtg
      481 gtgcccacct ccagggaagc attgatcacg ttggtggcgg cctggcccgc cttgtcgatg
      541 atcgttcgcc ttccgggact gaagagatga tcgaacctcg agtcgtagat gatgattggg
      601 tcgaggccct tgtcgtaata gaagatccgg tcccacttcc gcatgccttc cggattgtct
      661 ggttgccagg tcttcccgtg ggcctgcaac gccgcggaca tcgctcccag caatcggtaa
      721 ccgaacgcac ccgcgccatc ggcaagggga acacccggga cccaggagaa aagcacacag
      781 ggtcgggtca gatcgctttc cggctcgcgg gcgtcgacga tgagagcccc gtaaccggtg
      841 ggtatcgggt tcaccacgtc gatatcggtc tcccggttca gggccgaaag ccaggcgacc
      901 tcgtactcga tgttcgcccg ggagttgccg aatggatctc cgacccggag cacgagttgc
      961 ctgccatcgt tggtgacaac ccgatacatc acattggaat gagtcgccag atgctcgagc
     1021 tccctcacct caaacgggta gcgctcgagg acctggcgag ccagggggag gaggcgagag
     1081 gcctcggcgg cgattcgatg tgcatccatt agtgaaagac gacaggttgc ctacccgtca
     1141 attgttgtca acatctcgat caacccaact cacggctgtg cggcaatgat ctcgtcgagg
     1201 gcggacacga atctctgatt ctcccggtcg cgaccaaccg tgaccctgag ccacccccgg
     1261 gacatcgggc gaacgatcac tcctcgtctc atgaaatcat cgctcaccgc gccggaatct
     1321 tcgccgattc gcaggtagac gaagttggcg tgactctcag tgtggaagat tccccgctcc
     1381 tcgagaactc ccaagaggta atgacgtccg atggcattgg cctccacccg gcgtcggtac
     1441 tcggcctcgt cgcccagtga gaaggtcgcc gcggtctgtg cggcctggct gatgctgaac
     1501 gggagctgag tcttgcgcag ctcggcgatc gtcgccggct gcccaaccgc gtatcccacg
     1561 cggtgtgcgg cgagggcgaa gatcttggag aaggtgcgaa gcaccaccac attgggtcgc
     1621 tccattgcga gggaaacgga agtccggtac gagggatcgg ttacgaagtc ggaataggcc
     1681 tcgtccacca cgacaaggac ccgctccggg acagcac
//
LOCUS       DATITW010000421         1579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_701718, whole genome shotgun sequence.
ACCESSION   DATITW010000421 DATITW010000000
VERSION     DATITW010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701718"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..>1579
                     /locus_tag="VLS86_05925"
     CDS             54..>1579
                     /locus_tag="VLS86_05925"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HSO50065.1"
                     /translation="MGTALQDNRLVTITGPGGVGKTSLALKVATDLHESFADGVWVVE
                     VSRVADESLLAASVARQLRITESPHQPLIDTLASRMGRARALLILDGCEHLIGAVAKL
                     ADYLLSTTAELKILATSRELLSIRDELLVHVGPLPIPGPIAQSVSDIAASDAVALFVD
                     RARLVAPGFELDAQNASLVAEVCRRLDGIPLAIELAAARLKVLSVAQLVQRLDQQFAL
                     LSGTLRDMPPHQQTLETTLDWSYDFLSGAERLLFKRLSIFSGGFTLEAAEQVCSGDLV
                     APELVMNLLGRLVETSLVMTSVNDDQRYRLLEPISHYAKMRLDAEGRSDDLRDRHARF
                     YTALAEETDRQLLGRDQTPAADRLEQEHYNLRNALEWLFNSGDYEGALALAGALRWFW
                     VIKREAFEGTEWLERTLTKREGIPAAVVARALNGAGLMAIRRLDFKRAEVALAEGLDL
                     YRQVGDGQGEARQIYLLAVLAWFEDDLERAEHLITEAEDASRANGEDWALAWSLAVKG
                     T"
BASE COUNT          279 a          531 c          502 g          267 t
ORIGIN      
        1 acctcgcccg gcgcgatgtg caactcatcg gtcgaatcga ggaacaacgg agcgtgggga
       61 ccgccctcca ggacaatcgc ttggtcacca tcaccggtcc cggcggagtg gggaagacca
      121 gcctcgccct caaagtggcc accgacctcc acgagtcctt tgccgacggg gtgtgggtgg
      181 tggaggtctc gagggtggcg gacgagtcgc tcctggccgc cagtgtggcc cgtcagttgc
      241 gcatcaccga aagcccccac caaccgctga tcgacaccct ggcatcgagg atgggccggg
      301 ctcgggcgct tctcatcctc gacggttgtg agcatctgat cggcgccgtg gccaagctcg
      361 cggactacct gctgtcgacg accgccgagc tcaagatcct ggccaccagc cgcgagctcc
      421 tctcgataag ggacgagctc ctggtgcacg tcggcccgct gccaatccca gggccgatcg
      481 cccagtcggt cagtgacatc gccgcatccg acgctgtcgc actcttcgtc gatcgcgccc
      541 gtttggtggc gcccggattc gagctcgatg cccagaacgc ctccctggtc gccgaggtat
      601 gtcgccgtct cgacgggatc ccgctcgcca tcgaactggc tgcggcccgg ctcaaggtgt
      661 tgtcggtcgc ccaactggtg caacgtctcg atcagcagtt tgccctcctc tccgggaccc
      721 tcagggacat gccaccacat caacagacgt tggagaccac cctcgattgg agttacgact
      781 tcctctccgg cgctgaacga ctcctcttca aacgtctctc gatcttctcc ggtggcttca
      841 cgctcgaagc cgcggaacag gtctgctccg gcgacctggt ggctccggaa ctcgtcatga
      901 atctcctggg aaggctggtg gaaacctcgc tcgtcatgac cagcgtcaac gacgaccagc
      961 gataccggct tctggagccg atttcgcact acgcgaagat gcgcctcgat gccgaagggc
     1021 gaagcgatga ccttcgggat cgtcacgcca ggttctacac agcgctggcc gaggagaccg
     1081 accgccaact cctggggagg gaccagaccc cggctgccga ccgcctcgag caagagcact
     1141 acaacctgcg caacgccctc gaatggctgt tcaactcagg ggactacgag ggtgccttgg
     1201 cccttgccgg cgccctgcgc tggttctggg tgatcaagcg cgaggcgttc gagggaaccg
     1261 agtggctcga gcggacactg acgaaacggg aggggatccc cgccgcggtg gtggcgcggg
     1321 ccctcaacgg cgcgggcctg atggcgatcc gccgtctcga tttcaagcga gctgaagtcg
     1381 ccctagccga gggcctcgac ctctatcgcc aggtcggcga cggccaaggc gaagcgcgac
     1441 agatctatct cctcgcagtg cttgcctggt tcgaggacga cctcgagagg gcagagcacc
     1501 tgatcaccga ggcggaggac gccagccggg ccaacggcga ggattgggcc ctggcatggt
     1561 cactggcggt caaagggac
//
LOCUS       DATITW010000422         1886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_421320, whole genome shotgun sequence.
ACCESSION   DATITW010000422 DATITW010000000
VERSION     DATITW010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421320"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VLS86_05930"
     CDS             complement(<1..381)
                     /locus_tag="VLS86_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HSO50066.1"
                     /translation="MNSQPADGWRMVEKASELSRQGLEFALATVVWRQAPSSGHQGAR
                     AIITADGDMHGWIGGACAEPVVIREAQKVIEDRQPRLILLGTPDQFGELPEGMTFVQM
                     SCQSEGALELYIEPVIPSPHLVVIG"
     gene            complement(378..>1886)
                     /locus_tag="VLS86_05935"
     CDS             complement(378..>1886)
                     /locus_tag="VLS86_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSO50067.1"
                     /translation="RDVYLHGELALRKDGKILGVRMHTTADHGAFFSDAQPSKFKIGL
                     MHSAFACYDIPSAHLVARGVYTNKAPGGVAYRCSFRVTEAMFFQERMVQAAADDLGMD
                     QAEFRRINFVRDDDFPHRTPWGFLTDSGQYRKCLDLGLEAIGYQDFLQQKAEAAKRGR
                     LLGIGISTMTEPLGAGNSREYDILGIKMFDSAELKVHMTGKALLRTGAKSQGQGHETT
                     WAQIVAHELGIPAEDIFVEEGDTDTAPFGMGTYASRSTPVAGAAVAMVSRKIRAKARK
                     IAAHLLEISEEDVEWELGRFYVRSQPDRGVTIQECAAAAYSNMPDGMEPGLDNTAYYD
                     PPNLTWPFACYIATVEVDPETGVWDVLRLVAVDDCGVRINPMIVEGQIMGGLTEAYAM
                     ANMQFITFDAEGNCIGSNFTDYLLPTAWETPGFELHEVVTPCPHHPIGAKGIGECAAV
                     GGPAAFVNAVIDALKGTGVRNIDMPVFPDRVYEALQTGKDISGAPPVRTDEA"
BASE COUNT          307 a          615 c          616 g          348 t
ORIGIN      
        1 cccgatcacc accagatgag gactggggat caccggctcg atgtagagct ccagcgcgcc
       61 ttcggactga catgacatct ggacgaaggt catcccttcc ggcagctcgc cgaactggtc
      121 aggtgtcccg agcaggatga ggcgtggctg gcggtcctcg atgacctttt gcgcttcccg
      181 gatgaccacc ggctcggcac aggcgcctcc gatccagccg tgcatgtcac cgtcggccgt
      241 gatgatggcc cgcgcccctt gatgacctga cgacggcgcc tggcgccaca ccaccgtggc
      301 caaggcgaac tcgagaccct ggcgagacaa ctcgctggct ttctcgacca tcctccagcc
      361 gtcggcgggt tgggagttca tgcttcgtct gtcctgaccg ggggcgcccc gctgatgtcc
      421 ttgccggtct gcaacgcctc atagacccgg tcggggaaca ccggcatgtc gatgttgcga
      481 acgccggtgc ccttgagggc gtcgatcacg gcgttgacga aggcagccgg cccgcccacc
      541 gcggcgcact caccgattcc cttggctccg atcgggtgat gggggcaagg ggtcacgacc
      601 tcgtgcaact cgaagccggg ggtctcccac gccgtcggca gcaaatagtc ggtgaagttg
      661 gacccgatgc agttgccctc ggcgtcgaac gtgatgaact gcatgttggc catggcgtag
      721 gcctcggtga gaccgcccat gatctggccc tcgacgatca tcggattgat ccggaccccg
      781 cagtcatcca ccgccaccag gcggagcacg tcccacaccc cggtctcggg gtcgacctcg
      841 accgttgcga tgtagcaggc gaacggccag gtgaggttgg gcggatcgta ataggccgtg
      901 ttgtcgagac ccggctccat gccgtcgggc atgttggagt aggcggccgc agcgcactcc
      961 tggatggtca cccctcggtc gggctggctc cgcacataga agcgccccag ctcccactcg
     1021 acgtcctcct ccgagatctc caggagatgg gccgcgatct tgcgggcctt ggcccggatc
     1081 ttgcgcgaga ccattgccac tgccgccccg gccaccggag tgctccggga tgcgtaagtg
     1141 cccatgccga atggagcagt gtcggtgtcg ccttcctcga cgaagatgtc ctccgcaggg
     1201 atgccgagct cgtgggccac gatctgggcc caggtcgtct cgtgaccttg accctggctc
     1261 ttggctccgg tgcggagcag cgccttgccg gtcatgtgga ctttcaactc ggccgagtcg
     1321 aacatcttga ttccaaggat gtcgtactcc ctgctgttgc cggcgcccag cggctcggtc
     1381 atggtggaga tgccgatgcc gagcagacgg cctctcttgg ccgcttccgc tttctgctgg
     1441 aggaagtcct ggtagccgat ggcctcgaga cccaggtcga ggcatttgcg gtactggccg
     1501 gagtcggtga ggaatcccca cggggtgcgg tgggggaagt cgtcatcccg cacgaagttg
     1561 atccgacgga actcggcctg gtccattccc aggtcgtcgg cggcggcctg aaccatccgc
     1621 tcctggaaga acatggcctc ggtgacccgg aatgagcacc ggtaggcgac tccaccaggc
     1681 gccttgttgg tgtacacccc gcgcgccacg agatgggcgc tcgggatgtc ataacaggcg
     1741 aaggcagagt gcatcaggcc gatcttgaac ttggacggct gggcgtcgga gaagaaggct
     1801 ccgtggtcgg cggtggtgtg catcctcacc cccaggatct tcccgtcctt gcgcagcgcc
     1861 aactcgccgt ggagatagac atcgcg
//
LOCUS       DATITW010000423         1839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_381069, whole genome shotgun sequence.
ACCESSION   DATITW010000423 DATITW010000000
VERSION     DATITW010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381069"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VLS86_05940"
     CDS             complement(<1..892)
                     /locus_tag="VLS86_05940"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSO50068.1"
                     /translation="MVAWFADLSNFSHLTHEITERERAGPELVGQLLNETFGQIIEQV
                     TSYGGEVLEFAGDSVLATWTINAERDRVQALKLASSCGLEVLELKLPEVYADRPLDLR
                     VGIGVGTGVLMEVGGVDQLWHFVVGGPPFSQIGLASNVARPGEVVLSPEATDSIGSAA
                     RGRVDEEGFLRLTQVDDGEPPNIARRSEMNDHLTTELRRFLPPPVLRRIDAGQSEWLA
                     EFRQVTSVFVNLPRLDPTTTSARDTLQDLVTKTQVALAKYEGTLTRILDDDKGVTLFA
                     SFGLPPFAHEEDPYLAVQAAE"
     gene            1071..>1839
                     /locus_tag="VLS86_05945"
     CDS             1071..>1839
                     /locus_tag="VLS86_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50069.1"
                     /translation="MPESNSWHPAEVGEESIRRIGRIVAGLGGELGKLAAHLLDRHGY
                     VIGSDGGGPHRNVLGHPLFELPIWLDRATSDRGELARDTLLEVCESSLCGYLSVRAED
                     DYFDGDWDDANGAMMLSGFFRARHNALLAPLVSDRRFWLRSESLWRAYGEAMLRERSL
                     HEADMQYGPEEFEMVLDRSQPLEIPGNAVLYMKGCWEMTGQHAELVRHLTRATQLFDD
                     FIDAPDDLAVGNFTWMVRRLGGREGGQALRRGMIAWCD"
BASE COUNT          347 a          564 c          587 g          341 t
ORIGIN      
        1 cctcggcagc ttggacggcc agatacgggt cctcttcgtg ggcaaacggg ggcagcccga
       61 aggaggcaaa cagggtgacc cccttgtcgt cgtcgaggat tcgtgtcagc gttccctcgt
      121 actttgccag cgcgacctgc gtcttcgtca ccaaatcctg cagcgtgtca cgcgcgctgg
      181 tggtggtcgg gtccagacgg ggaaggttga cgaacaccga tgtcacctga cgaaactcgg
      241 caagccactc gctctgtccg gcatcgatcc gtcggagcac aggtggcggg aggaatcgcc
      301 gaagttctgt ggtgaggtga tcattcatct cagaccggcg tgcgatgttc ggcggctccc
      361 cgtcgtcgac ctgggtcagg cgtagaaatc cctcttcatc gacccgcccc ctggcggcag
      421 aaccgatgga gtcggtcgcc tcgggtgaga gcaccacctc accggggcgg gcaacgttgc
      481 tggccaaccc aatctggctg aagggcgggc ccccgaccac aaagtgccaa agctgatcta
      541 ccccaccgac ctccatcaac acacccgtgc ccacaccaat gccgacgcgg agatcgagtg
      601 gccgatctgc atacacctcg ggcagcttca gctcgaggac ttccaagcca cagctggagg
      661 caagtttcag ggcctggacc cgatcacgct cggcgttgat cgtccaggtg gcgagcaccg
      721 aatcgcccgc gaactccaga acttctcccc cgtaactggt cacctgctcg atgatttgtc
      781 cgaacgtctc attgagcagc tggccaacga gctcaggccc ggctcgctct cgctcagtga
      841 tctcgtgcgt caggtgtgag aagttggaca ggtccgcgaa ccaggccacc atttcctgct
      901 gctcgcttgc cggtcgaact ggggatggga cttgtttagc gagccaactg atcagctggc
      961 cgggcacgaa accctgcagt acggtcgaat ctgtcgccat gaactccctg ggcgaagtga
     1021 gccaccccgc caaaggcaga acctagcaac ggctgggtta cagtccgatg gtgccggaga
     1081 gcaatagttg gcaccctgcc gaggtcggcg aagagtcgat caggcggatc ggccgcatcg
     1141 tggcgggact gggaggggag ctggggaagc tcgccgcaca tctcttggac agacacggct
     1201 atgtgatcgg gagcgacggg ggagggcccc atcgcaatgt ccttggtcac cccctgttcg
     1261 aactacccat ctggctcgac cgtgcgacca gtgaccgcgg tgagctggcc cgcgacactc
     1321 tgctcgaggt ctgcgagtcg agcctttgcg gttacttgtc ggtccgcgcc gaggacgatt
     1381 acttcgatgg tgactgggac gacgcaaacg gggcaatgat gctgtcgggg ttcttccgtg
     1441 cccgccacaa tgcgcttctg gcacccctcg tctccgaccg gcgcttctgg ttgcgctcgg
     1501 agagtctttg gcgagcatat ggcgaggcga tgctcaggga acgatcgctc catgaggctg
     1561 acatgcaata cggccccgaa gagttcgaaa tggtgctcga ccgatcccaa ccgttggaga
     1621 tcccgggcaa tgccgtcctc tacatgaagg ggtgttggga gatgacgggc caacacgccg
     1681 agctcgtgag gcacctgacc agagccactc agctgttcga cgacttcatc gatgcccccg
     1741 atgatctggc tgttgggaac ttcacttgga tggtcagaag gcttgggggt cgggagggag
     1801 gacaggcgct ccggcggggc atgatcgcat ggtgcgacc
//
LOCUS       DATITW010000424         1503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_261002, whole genome shotgun sequence.
ACCESSION   DATITW010000424 DATITW010000000
VERSION     DATITW010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261002"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VLS86_05950"
     CDS             <1..594
                     /locus_tag="VLS86_05950"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HSO50070.1"
                     /translation="PSSLELEEHSHAGMAARYVAGASGLPFAVLRAYVGSDLVKHTEV
                     ASILCPFTGEELAAVPALNPDVTVIHAQQADRQGNVGMWGITGVQREAVLAARTAIVT
                     VEEIVDELAHHGPNNVVLPSWTVSAVACVPQGSHPSYSMGYSTRDNAFYTDWDQISRD
                     REVFARWIETFVIGTPDHSSYLTAVSAHPLGKEALGG"
     gene            632..1369
                     /locus_tag="VLS86_05955"
     CDS             632..1369
                     /locus_tag="VLS86_05955"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014290838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HSO50071.1"
                     /translation="MMTVTASRKLQDGQVCFVGIGLPSEAANLARLSHAPNVVLIYES
                     GTIGTRPEILPLSIGDGELAERADLVVSVPEVFAYWLQAGRIDVGFLSAAQIDRYGNL
                     NSTVVGCYEDPKVRLPGAGGAPEIMSSAGEVIVMLRHTERAFVPSVDFVTSVGFGRQG
                     EGRAGHVGAGPTVVITDLGVLQPDPNTHELALVAVHPGVEVGQVLSRTGWDLLVADDV
                     VTTEPPTRDEIQILRELKARTREAHSR"
BASE COUNT          252 a          464 c          512 g          275 t
ORIGIN      
        1 ccgtcgtcgt tggagttgga ggagcacagt cacgcaggaa tggcagcccg ctatgtcgcc
       61 ggagcttccg gtctcccgtt cgccgtgttg cgtgcgtacg ttggctcaga tctggtcaag
      121 cacaccgagg ttgcatcgat cctctgcccc ttcaccggtg aggagctcgc cgcggtgccg
      181 gcactcaacc ctgatgtgac agtgatccac gcccagcagg ccgaccgtca ggggaatgtc
      241 ggcatgtggg ggatcaccgg cgtgcaacgc gaagcggtcc tcgcggcgag gaccgcgatc
      301 gtcaccgtgg aggagatcgt tgacgagctg gcccaccacg ggcccaacaa cgtggtgctg
      361 ccctcatgga cggtttcggc agtggcctgt gttccccagg gctctcaccc ctcatattcg
      421 atgggctatt ccacgcggga caacgccttc tacaccgatt gggaccaaat ctcacgggat
      481 cgggaggtgt ttgcgcggtg gatcgagacc ttcgtgatcg ggactccgga ccattcgtcg
      541 tatctgacgg cggtcagcgc gcatccgttg ggcaaggagg cgttgggtgg ttgacctgcg
      601 gagccaggag atcacgtaca cggcggacga gatgatgacc gtcaccgcgt cgcgcaagct
      661 ccaggacggt caggtgtgtt tcgtggggat cgggctaccc agcgaggctg ccaacctggc
      721 tcgcctttcc cacgccccga acgtggtgtt gatctacgag tcgggcacca tcgggacccg
      781 gccggagatc ctccctctct cgatcggaga cggcgaactc gcggaacgag ccgatctcgt
      841 ggtgagcgtc ccggaggtct tcgcctattg gctacaggcc gggcgcatcg atgtcgggtt
      901 cctctcggcg gcccagatcg accgttacgg gaacctgaac agtacggtcg tcggttgcta
      961 tgaagatccc aaggtccgcc ttcccggagc cgggggagcg ccggagatca tgtcgtccgc
     1021 cggcgaggtg atcgtgatgc tgaggcacac cgagcgggcg ttcgttccgt cggtcgactt
     1081 cgtgacctcg gtcggtttcg ggcgccaggg tgagggcagg gccgggcacg tcggtgccgg
     1141 ccccaccgtc gtcatcactg atctcggcgt gctgcagcct gatcccaaca ctcacgagct
     1201 cgccctggtg gcggtccacc ccggcgtcga ggtcggccaa gtgctgagcc gcaccggttg
     1261 ggacctgctc gtagccgatg acgttgtcac caccgagccc ccgaccaggg acgagatcca
     1321 gatcctgaga gagctgaaag cacggaccag agaggcgcac tcacgatgag accattcgtc
     1381 tacgagccgt tgccggggcg ggtggtgttc ggcacgggga cgatcgccca tctcccgatg
     1441 gagttggagg cactcgaggc gagtcgggtt gtcctgatct cggacaacat ggccaagggg
     1501 gag
//
LOCUS       DATITW010000425         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_461343, whole genome shotgun sequence.
ACCESSION   DATITW010000425 DATITW010000000
VERSION     DATITW010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461343"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VLS86_05960"
     CDS             complement(<1..954)
                     /locus_tag="VLS86_05960"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HSO50072.1"
                     /translation="MFATAAAVLILEILAVRLLAPYLGVSLEVFTGVIGVILAGISVG
                     AWLGGRAADRGEPRRLIGPLLVAGGLAAIASPLLVDLVGPSTSTGPLSIVLITSIGFF
                     APAARLSAVPPVVVKIRLASLAETGSVVGSYSAIGTAGAIFGSFVTGFVLIAAFPTRP
                     IVIAIGIGLTLLGLALWSARKTPGVSATLIGLVVLVALVVVNDGPCQYETTYHCAEVV
                     VDDARPSGRLLVLDRGHNSYVDLDDPTHLEFRYIRVMVDVIDTETAPGPLQVLSIGGG
                     GFTFPAFLEATRPGTGHVVLEIDAALVDIGRERLGFEDQAEV"
     gene            1076..>2281
                     /locus_tag="VLS86_05965"
     CDS             1076..>2281
                     /locus_tag="VLS86_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSO50073.1"
                     /translation="MRQWKPILVLILVAACTPSTTVTTTVSTSSTSAPPTTTPPATTT
                     TAPVRPPTDQILEDLGGSLCPDSEFTCVTLEMPLDHFDPSEERTIPVTFAVMPATGER
                     AGAFVTATGGPGSSGIAVADSYTSALDPAIPEVYDTVFFDQRGVAMSGGLGCPVAAAS
                     YYRVDILTGLGLDEEALTTAAATFAADCVEEMGNPDVLPFLGTDQVAADLEAFRETLG
                     YEDVVIYGESYGTQVTQTYAAAYGDRVSRMVLDGTVDLTLDGFTFFEQQAAAFGATLQ
                     STLDYCGEDPFCVDDLGVGPDEAYDRMVALLVEEPLREDFPLPGGGFAERSFGLGDLE
                     VVASGQMYSEDDRMMFLRALAAYSGRSDLVPMLRLLYLNLGMDPVTETAIEDPTYSDA
                     IYYSVECLDY"
BASE COUNT          415 a          788 c          730 g          348 t
ORIGIN      
        1 cacctctgcc tggtcctcga agccgagtcg ctcgcgtccg atgtccacca acgccgcgtc
       61 gatctccaac acgacatggc cggttccggg tcgggtggcc tcgagaaagg cggggaaggt
      121 gaatcccccg cctcctatcg acaggacttg gagcggcccg ggggcagtct cggtgtctat
      181 gacgtcaacc atgacccgga tgtagcgaaa ctcgaggtgg gtcgggtcgt cgaggtcgac
      241 atagctgttg tgacctcgat cgaggaccaa cagccggcct gacggtcggg cgtcatcgac
      301 caccacctcg gcgcagtgat aggtcgtctc gtactggcag gggccgtcgt tgacgaccac
      361 caatgccacc agcacgacca gacctatcag cgtggcgctg acaccagggg tcttcctggc
      421 cgaccagagg gccagaccga gaagcgtcaa cccgatcccg atcgcgatca cgatggggcg
      481 ggtcgggaac gccgcgatca agacgaatcc ggtcacgaag ctgccaaata tggcgccggc
      541 ggtgccgatc gccgagtagc tgccgaccac cgaccccgtc tccgccaacg aggcgaggcg
      601 gatcttgacc accaccggcg ggacggcgga caaccgcgca gcgggggcga agaacccgat
      661 gctggtgatg agcacgatcg agagcgggcc ggtcgatgtc gaagggccga ccagatcgac
      721 gaggagaggc gaagcgatcg cggccagacc gccggccacc aacagcggcc cgatcaaccg
      781 acgcggctcg ccccgatctg cggcccgccc gcccagccat gcgccgaccg agatcccggc
      841 gaggatgaca ccgatgaccc cggtgaacac ctcgaggctg acgccgaggt atggcgccag
      901 caaccggact gcgaggatct ccaggatgag gaccgccgcc gcagttgcga acaccagaaa
      961 tcgggtgagg gccgcttaca ccgacgagga gtatggccga ccccacctgc tagcaacgga
     1021 gcggctcaca gcccccaagc cgggcagggc cgcccggcag tagtctgccg ccgatttgag
     1081 acagtggaag ccaatcctgg tgctgatcct ggtcgcggcc tgcacccctt cgaccactgt
     1141 gaccaccacc gtctcgacct cctccacctc ggctcccccg accacgacgc ctccggcgac
     1201 caccactacc gcgccggtgc gcccccccac cgaccagatc ctcgaagacc tgggaggaag
     1261 tctctgcccc gactccgagt tcacatgcgt caccctggag atgccactcg accacttcga
     1321 cccgtcggaa gagcggacga ttccggtcac attcgccgtc atgccggcaa cgggcgagag
     1381 agctggggca ttcgtaaccg ccaccggggg accgggcagc tccggtatcg cggttgccga
     1441 ttcctacaca tcagccctcg atccggcgat acccgaggtc tatgacaccg tcttcttcga
     1501 ccagcgtgga gtcgccatgt ccggcggcct cggctgcccg gtggcggctg cctcctatta
     1561 ccgcgtcgac atcctgaccg ggcttggcct ggatgaagag gcattgacca cggccgcggc
     1621 gacgttcgcc gctgattgcg tcgaggagat gggcaacccc gatgtcctcc ccttcctcgg
     1681 gacggatcag gtcgccgccg acctggaggc gttccgggag acgctcggct atgaggacgt
     1741 ggtgatctac ggggagagct acggcaccca ggtcacccag acctacgccg cggcctacgg
     1801 agatcgggtg tcgcggatgg tcctcgatgg gaccgtcgac ctcaccctcg atgggttcac
     1861 cttcttcgag cagcaggccg cggccttcgg ggcaaccctc cagtcgacgc tcgactactg
     1921 cggcgaggac ccgttctgcg tcgacgatct cggcgtaggg cccgacgagg cgtacgaccg
     1981 gatggtcgcc ctcctcgtgg aagagcctct ccgcgaggac ttcccgctgc caggcggagg
     2041 cttcgcggag agaagcttcg ggctgggcga cctcgaggtc gtcgccagcg gccagatgta
     2101 ctcggaggac gatcggatga tgttcctcag ggcgttggcc gcctactcgg gtcgcagcga
     2161 cctggttccg atgttgcggc tgctctatct caacctcggc atggacccgg tgaccgagac
     2221 ggccatcgag gaccccacct actccgacgc catctactac tcggtggagt gtctcgacta
     2281 c
//
LOCUS       DATITW010000426         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_61067, whole genome shotgun sequence.
ACCESSION   DATITW010000426 DATITW010000000
VERSION     DATITW010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61067"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      188..269
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            376..>2549
                     /locus_tag="VLS86_05970"
     CDS             376..>2549
                     /locus_tag="VLS86_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50074.1"
                     /translation="MSLARVAPRKGFERFLAILSAFAVFMSLMVVFAPVALAHHPEIS
                     ANQTCVDGKVTIAYESISWRTDGGSGSGHSNIVIEVQVNGAGSWAQVGSGSYTAGNNY
                     RFSGTFDAESYWGESIQVRARAVGAWDNGQGGGETRATSSFTVNQDCTDAVGVTANPQ
                     VCAVNQQGVPQGAVSFNVDPASGATIQVYANSNFTGPVGGPLEDDDVLNLNPGTYYWQ
                     ATAASGFELEGAGSGQFTIAPCSASTVVVSGECAVNDHGAALGSVQVTIDPDSGATVV
                     VSGPGGPYNFSGVGGSSELAPGSYSWQATAGPGFSLDGQTSGEFDIAPCEGSVSVTSG
                     VCVLGNGPLGAVEVDIDPDSAAVVTIYSDPAMTNAVATFADDGGTADLAPGTSYWSAA
                     AGGGFALSGETSGSFTIEPCAASVTVDSGVCSIDDSGTPVGSVQVTIDPDSGATVVVT
                     GPGGPFDFSGSGGSEGLAPGAYTWQATPGSGFVLTGDTGGGFNIEPCDVSVLVVSGAC
                     VVGDGPAGTVTVAIDETSGANVTVLDSESNTVAAFTGAGGSSSLPPGSYTWQATPGPG
                     FEFPDGQAASGEFTIEPCEGSVVVSHGNCVAGAATAFGSMTVVIDPASGAVVTVFNAG
                     GLEVANFDGSGGSRALTAGTYTWTADAATGFGLVGESDGQFTVIACEDEVLDDEIVDD
                     EVDDLVVLPFTGARTETLLGASIVLLGSGLLLIRSSRRRDEAL"
BASE COUNT          420 a          772 c          883 g          474 t
ORIGIN      
        1 ggagagaaga ggggtagccg actcctcgtc aattcccgtt tgatgggatg agggcccggg
       61 tgatcgaccc gggccctcac gcgtccctgg cctgtggaaa acacctgagt cgggcgaccc
      121 gttgacacct gcccccccac aatgcagtat gtcgtggctg gccaccctca gtggccggca
      181 cggttagggg attttgggag gcttcgaaac cagacctgca aaacggtcac cttggtctgg
      241 tggagctaat ggtgagaccg gcctgtcccg ggcaacgaag ggataggaca atatggggct
      301 ggagacgact gctgtcgtct ggggcgaggg aaccacgccg tgccattggc gcgtcgcgcg
      361 cgtcgggggt cagacatgag cttggctcga gtcgctccac gcaaggggtt cgaacgtttc
      421 ctggcgattc tctccgcatt cgcggtattc atgtctttga tggtggtgtt cgcgcccgtg
      481 gcgctggcac atcatcccga gataagtgca aatcagacct gtgtcgacgg caaggtgacc
      541 atcgcctacg agtcgatcag ttggcggacg gatggcggct ccggttcggg gcattcgaac
      601 atcgtcatcg aagtgcaggt gaacggcgca gggtcgtggg cccaggtcgg cagcggctcg
      661 tataccgccg gcaacaacta ccggttcagc ggcaccttcg acgccgagtc gtattggggt
      721 gagagcatcc aggtgcgcgc ccgggcggtc ggagcttggg acaacggcca gggaggcggt
      781 gagacccggg cgacgagctc gttcacggtc aaccaggact gcaccgacgc ggtaggcgtc
      841 actgccaacc cccaggtgtg tgctgtgaat cagcaaggag tgcctcaggg agcggtctcg
      901 ttcaacgtcg acccggcttc gggcgccacc atccaggtgt acgccaactc caacttcacc
      961 ggcccagtcg gtggtccgtt ggaagacgat gacgtgctga atctaaaccc tggcacctat
     1021 tactggcaag ccacagcggc atcgggcttc gaactcgagg gcgcgggatc cgggcagttc
     1081 accatcgcgc catgctcggc gtcgaccgtg gtggtatcgg gagagtgcgc ggtcaacgac
     1141 catggcgctg cacttggttc ggtccaggtc acgatcgatc ccgactccgg ggcgactgtc
     1201 gtggtcagtg gtccaggtgg gccttacaac ttctccgggg tcggcggctc ctcagagctc
     1261 gcacccggtt cctacagttg gcaggcaacg gctggacccg gcttctcgct ggatggtcaa
     1321 acctccggcg aattcgatat cgctccttgt gagggctcgg tgtcggtgac cagcggtgtc
     1381 tgtgtcctcg gcaacggccc ccttggcgca gtcgaggtcg acatcgatcc cgactcggcg
     1441 gcagtggtga ccatctactc cgatccggcg atgaccaatg cggttgcgac cttcgccgac
     1501 gacggtggca ctgcggacct ggctccgggg acctcctact ggtcggcggc cgccggcggg
     1561 ggattcgcct tgtcgggcga aacgtcgggc tcgttcacca tcgagccgtg cgctgcctcg
     1621 gtgactgttg actctggcgt ctgttctatc gacgacagtg ggacaccggt cggctcggtc
     1681 caggtgacga tcgaccccga ttcgggtgcg acggtggtcg tgaccggtcc gggtgggccg
     1741 ttcgacttct ccggaagcgg cggatcagag ggattggcgc ccggcgccta cacgtggcag
     1801 gcgacgcccg gaagcgggtt cgtgctcacc ggagacaccg gcggtgggtt caacatcgag
     1861 ccgtgtgacg tgtcggtgct ggtggtcagt ggggcttgcg tcgtcggtga cggaccggcg
     1921 ggcacggtga ccgtggccat cgacgagaca tcgggtgcga acgtcacagt gctcgacagc
     1981 gagtcgaaca cagtggctgc tttcaccggc gccggtggct ccagttccct cccgccaggc
     2041 tcctacacgt ggcaagccac gcctgggccc ggattcgagt tcccggatgg tcaggcggcc
     2101 tccggggagt tcaccatcga gccatgtgaa ggctctgtgg tggtgagcca cgggaactgc
     2161 gtcgcaggcg cagccaccgc attcggttcc atgacagtgg tcatcgaccc cgcatcgggg
     2221 gcggtggtga cggtgttcaa cgccggtggc ctcgaggtcg ccaacttcga cgggagcggc
     2281 ggaagtcgtg ccctgacagc aggcacctac acctggactg ctgacgccgc aaccggcttc
     2341 ggcctcgtcg gtgagagcga cggccagttc acggtcattg cctgtgagga cgaggttctc
     2401 gacgacgaga tcgtggacga cgaggtcgac gacctggtgg tcctgccgtt caccggagcc
     2461 aggaccgaga ccctcctcgg cgcctcgata gtgctgttgg gatcgggtct gctcctgatc
     2521 cgctcctcga gacgacgtga cgaagcgct
//
LOCUS       DATITW010000427         1602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_621832, whole genome shotgun sequence.
ACCESSION   DATITW010000427 DATITW010000000
VERSION     DATITW010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621832"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VLS86_05975"
     CDS             complement(<1..488)
                     /locus_tag="VLS86_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010887561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSO50075.1"
                     /translation="MGRGNAIEREVSDEAIRQYLDGIGTYHLLTAEDEVALAKAIENG
                     RKAEIALESERDPVKRRTLHEMVHAGEEARQTFIRCNLRLVVSIAKRYGHAKLPFLDL
                     VQEGNLGLIRAVEKFEYRKGFKFSTYATWWIRQAITRAIADMSRTIRVPVHMMDTITQ
                     MQT"
     gene            complement(644..>1602)
                     /locus_tag="VLS86_05980"
     CDS             complement(644..>1602)
                     /locus_tag="VLS86_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50076.1"
                     /translation="YANLEVIDRIQPDSTAGAWLNGLLCVGAGGDPTLAIKTLSGVEH
                     SSLVLYTEGDSEVLAEDVERLLDTAAGPVVIGSDGVIYAIDVDNRTLTPLTESVGDLR
                     GQLVAAVSSPDGTHIALTTLDWDQYPLDGRVIVIDLKQGTNAQLGVACDIYPIWLDDE
                     TLTFTDHCTSEVPGFYDTNLEAVAGNPIPDYAHTSYVTDETHTIFYPSEFAIRALEPG
                     MEDSVELTRLFTYPGALLLMPPSARTEWVQPAFDPAPIVDVPPVTFVEAPAPGVPGAD
                     PVDAETPIWLLIVVAIAAGGGLWMLLRRPGETPASEVWRETL"
BASE COUNT          291 a          473 c          526 g          312 t
ORIGIN      
        1 gtctgcatct gggtgatggt gtccatcatg tggaccggaa cccggatggt tcggctcatg
       61 tcggcgatgg cacgggtgat cgcctgccga atccaccagg tggcataggt ggagaacttg
      121 aatcccttgc ggtactcgaa cttctcgacg gcacggatca agccgaggtt gccctcctgg
      181 accaggtcga ggaagggaag cttggcgtgc ccgtagcgct tggcgatgga gacgacgaga
      241 cgaaggttgc accggatgaa agtctggcgg gcttcttcgc cggcgtgaac catctcatgg
      301 agggtccggc gcttgaccgg gtcccgctcc gattcgagtg cgatctcggc cttgcggcca
      361 ttctcgatgg ccttggcgag agctacctcg tcctcggcag tgagaaggtg atacgtgcca
      421 attccgtcca gatactggcg gattgcctcg tcgcttactt ctcgttcgat ggcgtttcct
      481 ctacccatgg tccttcgagc ctccagccag cgtaggtaca catctggtca gtcctgggga
      541 ttgtcaacag gggtatcgac cgattatcca caacgggtaa ataggtctag cgacctactt
      601 gtcagccgtt ttcgcaggct ctgattgacg gtttttcgac caatcagagc gtttcgcgcc
      661 agacctcgga ggctggcgtc tcgccggggc ggcgcaagag catccagagc ccgcccccgg
      721 cggcgatcgc aaccactatg agcagccaga tcggagtctc cgcatcgacc ggatcggccc
      781 cgggcacccc gggcgccggc gcttcgacga aggtgaccgg aggcacgtcg actatcgggg
      841 cggggtcaaa tgcgggctgg acccactctg tgcgcgccga cggcggcatc aagaggaggg
      901 caccggggta ggtgaagagc cgggtcagct caacgctgtc ctccatcccc ggctcgagag
      961 cccggattgc aaactcgctg gggtagaaga tggtgtgcgt ctcgtcggtg acatacgacg
     1021 tatgggcgta atcaggaatc gggttcccgg ccacggcttc cagattcgtg tcgtagaaac
     1081 ccggcacttc ggaggtgcaa tggtcggtga aggtgagggt ctcgtcatcg agccagatcg
     1141 gatagatgtc acaagccacc ccgagttgcg cgttggtccc ctgcttcaga tcgatgacga
     1201 tcacgcgtcc gtcgagcgga tattggtccc agtcgagagt ggtgagggca atgtgggtcc
     1261 cgtccgggga agaaacggcc gcgaccagtt ggccgcgcag atcgcccacc gactcggtca
     1321 gcggagtcag ggtgcgattg tcgacgtcga tggcgtagat gacaccgtcg ctaccgatca
     1381 ccaccggccc ggcagcggta tcgagcagtc gttccacgtc ctcggccagg acctcgctgt
     1441 cgccctccgt atagaggacc agtgagctgt gttccacccc cgagagggtc ttgatggcca
     1501 atgtcggatc tccaccggct ccgacgcaga gaagcccgtt gagccaggcc ccagcggtgg
     1561 agtcgggttg gatccggtcg atcacctcca ggttggcata cg
//
LOCUS       DATITW010000428         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_501378, whole genome shotgun sequence.
ACCESSION   DATITW010000428 DATITW010000000
VERSION     DATITW010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501378"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VLS86_05985"
     CDS             <1..673
                     /locus_tag="VLS86_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50077.1"
                     /translation="ATFAAGAVRCNGGARVVAAEVRRGVFTGIDAEPVETSMGRYEIM
                     TTKGSTPEELWLGIQERMGDLEGQVAESQAGAALVVVDGPLSHRRHLEGAVGYVKTQH
                     VQYLPDELRATLAGLPVGYRTPLFLTTTSWSRFSWYLRLANGTGPAGGLVRCEVKADM
                     TVADAARMAHLVSASLPRYASARHKDPRAPQNLYPIGGLERELRRRLGDRDLAMRALR
                     VAAAA"
     gene            complement(677..1072)
                     /locus_tag="VLS86_05990"
     CDS             complement(677..1072)
                     /locus_tag="VLS86_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50078.1"
                     /translation="MKAEESRTRIAVSVVAPALLLLVAWGFWLVDAAMPLIAIVLVLA
                     VLLGYVVAFDFPTSIEVESTGINRVCLFRRHVLQWDDILGIVQPRRGGLVVMTKDRKR
                     HVLLDRLLEPEELDVLRAEAERNGVRIEF"
     gene            complement(1069..2022)
                     /locus_tag="VLS86_05995"
     CDS             complement(1069..2022)
                     /locus_tag="VLS86_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSO50079.1"
                     /translation="MGNRLLTTSHGTNREAFSAGDWARFIAVSGIWGASFLFIAIGLD
                     AFHPGLVTWLRVGFGAAFMATLAKARNTRISRQDLPRIGLLGLIWVAIPLTLFPIAQQ
                     WIDSAVAGMLNGAMPMFTAIVASLLLRQLPGRLQMVGLVVGLAGILAIALPSAESRST
                     AALGVTLVLVATVCYGVATNIVVPLQQKYGSIPVMARVLWVALLLVTPYGIYGLTESG
                     FAWQSLAATMAVGVLGTGVAFILMGSLVGSVGPTRSSFITYLMPVVALVLGVVFRDEI
                     VAPLAVVGSLLVIVGALLASRREAPLSTSPSTDEAAVGPRA"
     gene            complement(2026..>2528)
                     /locus_tag="VLS86_06000"
     CDS             complement(2026..>2528)
                     /locus_tag="VLS86_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSO50080.1"
                     /translation="GSPASGIVMAVRRDSPLSVAVTLVDGVVVARSGFQSSINYRSVV
                     IHGHGSVLRDAEHERALDLLVEGLIPGRLADVRRPTQQELRKTSVVSLPIDVLSAKVS
                     TGPPEDEVFDLGTGVWAGVVPVTVSFGEPVPSPDLEPGLHTPDYLTAFRRALPLMQLR
                     PLVWCS"
BASE COUNT          466 a          832 c          864 g          366 t
ORIGIN      
        1 cgcgaccttt gctgcaggcg cggtgcggtg caacggcggt gcccgggtcg tcgccgccga
       61 ggtgaggaga ggggtgttca ccggcatcga cgccgagcct gtcgagacct cgatgggccg
      121 atacgagatc atgacgacga aaggctcgac gccggaagag ctttggctcg ggatccagga
      181 gcgcatgggc gatctcgagg gccaggtcgc cgagagccag gccggcgctg ctctggtcgt
      241 ggtcgatggc ccgctctccc atcgccgcca cctcgagggc gccgtcggct atgtcaagac
      301 ccagcatgtg cagtacctgc ctgacgagtt gcgggccacg ctcgccgggc tcccggttgg
      361 ctaccggacc cccttgttct tgacgaccac gtcatggagc cgattctcct ggtacctgag
      421 actggccaac gggaccggcc cggcgggggg tctcgtccga tgtgaggtga aggccgacat
      481 gacggtggcc gatgccgccc gaatggcgca cctggtgagc gcatccttgc cccgatacgc
      541 ctcggcgcgc cacaaggacc ctcgcgctcc ccagaacctc tatccgatcg gcgggctgga
      601 gcgggagctg cggcgacggc tcggcgatcg agacctggcg atgcgcgccc tcagggtggc
      661 ggcggcggcc tgagtctcag aactcgatcc ggaccccgtt gcgttcggcc tcggctcgaa
      721 gcacgtccag ctcctccggc tcgaggaggc gatcgaggag gacgtggcgc ttgcggtcct
      781 tggtcatgac caccagcccc cctcgtctcg gttggacgat gccgaggatg tcatcccact
      841 gcaacacgtg gcgtcggaag agacagactc ggttgatgcc ggtggattcc acctcgatcg
      901 aggtcgggaa atcgaaggcg accacatacc cgagcaggac ggccagcacc aacacgatcg
      961 cgatcagtgg catcgcggca tcgaccaacc agaaccccca ggccaccagc agcaacaggg
     1021 ccggcgccac caccgagacg gcgattcggg ttcgcgactc ttcggccttc atgctcgggg
     1081 ccccaccgct gcctcatccg tgctcggcga cgtcgagaga ggggcttcgc gccgggacgc
     1141 gagcagggcg ccaacgatca ccagcagcga gccgacgacc gcgaggggtg cgactatctc
     1201 gtccctgaag accactccca gcaccagcgc cacgaccggc atcaggtagg tgatgaagct
     1261 cgacctggtg gggccgacgc ttccgaccag cgagcccatc aggatgaagg cgacaccggt
     1321 tcccaggact ccgactgcca tcgtggcggc cagtgattgc caggcgaatc cggattcggt
     1381 gagaccgtag atcccatacg gcgtgaccag caggagagca acccaaagga cgcgggccat
     1441 gaccgggatc gagccgtatt tctgttggag cggcacaacg atgttggtgg cgacgccgta
     1501 gcaaaccgtc gcgaccagga ccagggtcac tccgagggcg gctgtgctcc tgctttccgc
     1561 cgaggggagg gcgatggcga ggatcccggc cagacccacc accagcccga ccatctgcag
     1621 acgtcccggc agttggcgga gcaggagcga ggccacgatg gccgtgaaca tcggcatcgc
     1681 cccgttgagc atcccggcta ctgccgagtc gatccactgt tgggcgatcg ggaaaagcgt
     1741 cagggggatc gccacccaga tcagacccag cagcccgatc cgtggcaggt cctggcgcga
     1801 gatgcgcgtg ttccgcgcct tggccagggt ggccatgaat gccgcgccga agccaacccg
     1861 caaccatgtc accaaccccg ggtgaaaagc gtcgagcccg atggcgatga agagaaagga
     1921 cgctccccag atgccgctca ccgcgatgaa ccgagcccag tccccggcgc tgaacgcctc
     1981 gcgattggtg ccatgcgatg tggtgagcag tcggttgccc atggtctaag aacaccagac
     2041 gagtggacgg agttgcatca agggtaaggc acgccggaaa gcggtcagat aatccggggt
     2101 gtggagcccg ggctcgagat ccggcgacgg gaccggctcg ccgaaggaca cggtcacggg
     2161 caccacgccg gcccagacac cggtgccgag atcgaacacc tcgtcctcgg gtggccccgt
     2221 ggagaccttg gcggagagca cgtcgatcgg gagtgagacg accgaggtct ttcgcagctc
     2281 ctgttgggtg ggtcgccgca catcggcgag acggccgggg atgagcccct cgaccaggag
     2341 gtcgagcgcc cgctcgtgct cggcgtcccg gaggaccgac ccgtggccgt ggatgacgac
     2401 cgaccggtag ttgatcgaag actggaaccc gctcctggcg actaccaccc cgtcgacgag
     2461 ggtgactgcg acgctcagag gcgagtcgcg tcggactgcc atgacgatgc ctgacgccgg
     2521 cgacccgt
//
LOCUS       DATITW010000429         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_180885, whole genome shotgun sequence.
ACCESSION   DATITW010000429 DATITW010000000
VERSION     DATITW010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180885"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VLS86_06005"
     CDS             complement(<1..711)
                     /locus_tag="VLS86_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSO50081.1"
                     /translation="MVGKTVLITGGTGGIGKAAAIGLASMGARVGITGRDRARAEQAA
                     AVIARESGNPAVDVFVADMSSQSEVRRLSGEVLAAYPRLDVLLNNVGGFWAHRHLTAD
                     GLEHTFALNHLAPFLLTNLLLERLVASTPARVVTVSSGAQSMGKIDFDDLMGESDYSG
                     QRAYNQSKLANVMFTFELAERLEGSGVTATVLHPGMTNTAFSGEDPARAMALLVVLAR
                     PFMRSPKRGADTAVYLASS"
     gene            complement(775..1746)
                     /locus_tag="VLS86_06010"
     CDS             complement(775..1746)
                     /locus_tag="VLS86_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HSO50082.1"
                     /translation="MRAIVQDAYGSTEVLRLAEIDRPEIGENEVLVEVAAAGMDRGTW
                     HLMAGKPYLMRIIGFGFRRPKNRVPGLDVAGTVVAVGSEVTRFKPGDEVFGMSRGSFA
                     GYAAAREDKLARKPGSLSFEQAAVVPISGGTALQALRDSGRIEDGQSVLIIGASGGVG
                     TYATQLAKTFGAEVTGVCSTSKVDLVRSIGADHVIDYTREDYLDGSRRYDLILDIGGN
                     NGLSRLRSGLTPNGTLVIVGGEEGGNLTGGFGRQIRALALSPFVGQRLVMLASKERHT
                     DLEVLSPLLQSGQVVPVIDRTYSLAEVSDAMRHLEAGEARGKIAITV"
     gene            1974..2645
                     /locus_tag="VLS86_06015"
     CDS             1974..2645
                     /locus_tag="VLS86_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014923168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSO50083.1"
                     /translation="MTTAVGVGETKRLPLNRERLLHAAVGVADAGGVAALTMRSLAHE
                     LGVKPMSLYHHVANKDEILDGMIDIVFGEIDLPPVDGDWRTAMRHRAVSARRVLTRHP
                     WATPLMESRANPGPATLRHHDHMIGTLRRAGFSIQMTAHAYSLLDSYVYGFALQEASL
                     PFDRETAPEVAGSILASSPGDAYPHLAELATEHVLKPGYDYGDEFEFGLELILDGLEK
                     LRGSD"
     gene            complement(2659..>3067)
                     /locus_tag="VLS86_06020"
     CDS             complement(2659..>3067)
                     /locus_tag="VLS86_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HSO50084.1"
                     /translation="GASYLVWLGLQTLWATRRRARQVPMREPETWSMPGIGGAPLLQG
                     VLTNVLNPKLAVFFLSFLPQFVDGARPATPQILVLTGTFIVMGAIWLASYVIAVDQLS
                     GVLTRPVVKRWLDRFIGTTLVALGLRLAMVETD"
BASE COUNT          551 a         1028 c          954 g          534 t
ORIGIN      
        1 tgagctcgcc aggtacaccg cggtgtcggc acctcgtttc gggcttctca tgaacggtcg
       61 ggcgagcacg acgaggaggg ccatggcccg agcgggatcc tccccgctga acgcggtgtt
      121 cgtcatgccg gggtggagca cggtcgcggt gacgcctgaa ccttcaagac gctctgccag
      181 ctcgaaggtg aacatcacgt ttgccaactt cgactggttg taggctcgct gccccgagta
      241 gtcgctctcc cccatgagat cgtcgaagtc gatcttcccc atcgattgcg ccccggacga
      301 cacggtgacg acacgagccg gcgtgctggc aacgagtcgc tcgaggagga ggttcgtcag
      361 cagaaatggc gcgagatggt tgagggcaaa cgtgtgctcc agcccatccg cagtcaggtg
      421 gcgatgtgcc caaaatcccc cgacgttgtt caacagcacg tcgaggcgcg ggtaggcggc
      481 gagaacctcg ccggacaatc gccgaacctc gctttgcgag gacatgtcgg caacgaagac
      541 gtccaccgca gggttgcccg actcgcgggc gatcacggct gcggcctgct cggcgcgggc
      601 ccggtcccga ccggtgatgc ccaccctggc gcccatcgag gcgagcccga tcgcagccgc
      661 cttcccgatc ccaccggtgc caccggtgat cagcacggtt ttgccgacca tcggggatgt
      721 ctcatccatt gccagttccc ttcgtcgaat ggctcgtgga atcgagctgt gcgatcagac
      781 ggtgatggcg atcttgcccc gcgcctcccc ggcctccagg tgtctcatcg catcggaaac
      841 ctcggcgagc gagtaggtcc tgtcgatgac ggggacgacc tgaccgctct ggagaagcgg
      901 gctgaggact tccagatcgg tatgacgctc cttggatgcg agcatcacca gccgctgacc
      961 cacgaacggc gatagtgcca gggcacggat ctggcggcca aagcctccgg tgagattgcc
     1021 gccctcttcc ccaccgacga tgacgagggt cccgttgggt gtgagcccac tccgcaatcg
     1081 ggagagaccg ttgttgccgc cgatgtcgag gatgaggtcg tagcgcctgg agccgtcgag
     1141 gtagtcctct cgggtgtagt cgatgacatg atcggcgccg atcgaccgga cgagatcgac
     1201 tttcgaggtg ctgcacaccc cggtcacttc tgccccgaac gtcttggcga gttgggttgc
     1261 gtaggtgccg acccctcccg acgcaccgat gatgagcaca ctctgcccgt cctcgatgcg
     1321 cccggaatcc cgcagcgcct ggagcgcggt tcccccggag atggggacga ccgccgcctg
     1381 ctcgaagctg agactgcccg gcttgcgggc aagcttgtcc tcgcgtgccg ccgcgtaccc
     1441 ggcgaacgag ccccgactca tgccaaacac ctcgtcgccg ggcttgaacc tggtcacctc
     1501 ggacccgacc gccacgaccg tcccggccac gtccagtccg ggcacgcggt tcttgggcct
     1561 ccggaatcca aacccgatga tccgcatcag atagggcttg ccggccatca agtgccaggt
     1621 gccgcgatcc atccccgcag ccgccacctc gaccagcacc tcgttctccc cgatttcggg
     1681 ccgatcgatc tcggctagcc gcaacacctc ggttgagccg taggcatcct gaacgatcgc
     1741 ccgcatcaga tccgtctcgc ctccgacgct cggagccgtc gtgtttaccc gggcgggatg
     1801 tgctcctcga gttggtccca tggtctgtcc ctctttctgt tgaaacggag gcaccgcccc
     1861 caaccgcatg accgtactaa gtacgcttac taagtacggt agacgctatg ctctggtttg
     1921 gcaagaaaac cctctccgct ccattccgga gtcggacacg gatcaggaaa tgaatgacga
     1981 cagcagtcgg cgtaggtgag acgaagcgac tcccgctcaa ccgggagcgg ctgctgcatg
     2041 ccgcggtcgg tgtggctgac gcaggagggg tggcagcgct caccatgcgg tccctcgccc
     2101 atgagctcgg ggtgaagccg atgtcgctct atcaccacgt cgccaacaag gacgagatcc
     2161 tcgacggcat gatcgacatc gtcttcgggg agatcgacct cccccctgtt gatggcgatt
     2221 ggcgcaccgc aatgcgtcac cgcgctgtct ccgctcggcg cgtcctcacc cgtcaccctt
     2281 gggcaacccc gctcatggaa tcgagggcca accctgggcc ggccacgctg cgacaccacg
     2341 accacatgat cggcactctc cgccgagccg gcttctcgat ccagatgacc gcccacgcct
     2401 actcgttgct ggacagctac gtctacggct tcgctcttca agaagccagt ttgccgttcg
     2461 acagggagac cgccccggaa gtggcgggat cgatcctggc cagttcccct ggcgacgcat
     2521 atccgcacct agccgagttg gcgaccgagc acgtgctcaa gcctggctac gactacggcg
     2581 acgagttcga attcggtctc gaactgatcc tcgacggact cgagaagctc cgcggatccg
     2641 attgacgacg ttcgttcgtc agtcggtttc gaccatcgcc aggcggaggc cgagcgcgac
     2701 gagggtggta ccgatgaatc ggtccaacca tcgcttgacc acgggtcggg tcagcacccc
     2761 cgaaagttga tcgaccgcga tgacataact ggcgagccag atcgctccca tgacgatgaa
     2821 ggtgccggtc agcaccagga tctggggggt ggccggtctg gcgccgtcca cgaactgagg
     2881 cagaaacgac aggaagaaca cagcgagctt tggattcagg acgttggtga ggacgccctg
     2941 gagcaggggg gcgccgccga tcccgggcat cgaccacgtc tccggctccc gcatcggaac
     3001 ttgccgggct cggcgccggg tcgcccagag ggtctgcagg ccgagccaca ccagatagga
     3061 ggcacca
//
LOCUS       DATITW010000430         1638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_661659, whole genome shotgun sequence.
ACCESSION   DATITW010000430 DATITW010000000
VERSION     DATITW010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661659"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VLS86_06025"
     CDS             complement(<1..300)
                     /locus_tag="VLS86_06025"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSO50085.1"
                     /translation="MTTVSASPSVFARSVPETMDALALLGPDQFEVQQVEVPQPAAGE
                     VLCRVHSVAVCGTDTEIISGHFLERGWPRSYPFTPGHEWSGEIVALGDEVAGFGFA"
     gene            complement(467..>1638)
                     /locus_tag="VLS86_06030"
     CDS             complement(467..>1638)
                     /locus_tag="VLS86_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HSO50086.1"
                     /translation="LIRAHDETELLRDVCRIVVEAGGYALAWVGYPVDDEEKNIEAQA
                     QWGEGRGYVENLELTWRDEGEGRGPAGTAIRDRKPYLVQDTAYDPDVIWHARPHRHGF
                     MSLVSVPMFYDDEVAGVLTVYDNQPGAFNDRGVAVLQRFADDLAYGIAAIRARKRQHE
                     AESKLRDLLESKDELIATIAHELRTPLAGVVGFAQVLRDDADTLDPESRAEMIRLVAE
                     QGMDLTNIVDDLLVAAKSEAGTLVIARVRVDLRAQAAQVLEAWGSDVVGAIDVSGSPA
                     VTTGDPARVRQILRNLVTNALRYGGEQIRVRVGGDESTVFVTVEDNGHGVDTEEQDRI
                     FEPYRRAHDAPGVTASMGLGLSISRQLAGLMGGDLIYRREPELSVFTLTMPAFAD"
BASE COUNT          278 a          549 c          537 g          274 t
ORIGIN      
        1 agcaaagccg aagccggcca cttcgtctcc aagggcaacg atctcccccg accattcatg
       61 acctggtgtg aacggatagc tgcgcggcca gccgcgctcg aggaaatggc ctgagatgat
      121 ctcggtgtcg gtaccacaaa ccgccacgga gtgcacccga cacaggacct ccccggctgc
      181 aggttgagga acctcgacct gctggacctc gaattggtcg gggccgagga gagcaagtgc
      241 gtccatggtc tccggaaccg accgggcgaa gaccgagggg gaagcagaga ctgtggtcat
      301 gatcgtcctt tcaacaggct cgacgtgcac ggagtcagtt cttgggtacg accctggcga
      361 gcgaatcgcc gggcgtctag ggacctaagt cacctcgggg aagttacaga gacgaggggg
      421 gccgggccgt cggcgggacg gattgaggcg gttagcgctc cgcgggtcag tcggcgaagg
      481 cgggcatggt cagcgtgaac acgctgagct ccggctccct gcgatagatc aggtcgcccc
      541 ccatcagtcc ggccagctga cgggagatgg aaagaccgag acccatcgag gcggtgaccc
      601 cgggggcgtc gtgcgctctc cggtagggct cgaagatccg atcctgttcc tcggtgtcga
      661 ccccatgacc gttgtcttcc acggtgacga acacggtcga ctcgtcgcca cccacccgca
      721 cccggatctg ctcccctcca tagcggagtg cgttggtcac caggttgcgc aggatctgac
      781 gaacccgggc cgggtctccg gtggtgaccg caggcgagcc gctgacgtcg atggccccga
      841 ccacatcgct cccccacgcc tcgagcacct gcgccgcctg ggcgcgcaga tcgaccctga
      901 ccctggcgat caccagagtc ccggcctccg acttggctgc gaccaggagg tcgtcgacga
      961 tgttggtcag gtccatgccc tgttcggcga cgagtcggat catctccgcc ctcgactcag
     1021 ggtcgagagt gtcggcgtcg tcccgcagca cctgggcgaa gccaaccact ccggccagcg
     1081 gggtcctcaa ctcgtgggcg atggtggcga tgagctcgtc tttggactcg aggagatccc
     1141 tcagcttcga ctcggcctca tgctggcgct tgcgggcccg gatggccgcg atgccgtaag
     1201 cgagatcgtc ggcgaaccgc tgcaagaccg cgaccccgcg atcattgaag gcccccggct
     1261 ggttgtcgta gacggtgagc accccggcga cctcgtcgtc gtagaacatc gggaccgata
     1321 ccagggacat gaacccgtgt cggtggggac gggcgtgcca gatgacgtcc gggtcgtagg
     1381 cggtgtcctg gaccagatac ggctttcgat cccggatcgc ggtgcccgcc gggcctcttc
     1441 cttcgccctc gtcccgccag gtgagctcga ggttctcgac gtagccccgt ccctcgcccc
     1501 actgcgcctg ggcctcgatg ttcttctcct cgtcgtcgac ggggtagccg acccaggcca
     1561 gggcataacc gccggcctcc accacgatcc ggcacacatc ccggagcaac tccgtttcgt
     1621 catgggcccg gatcaggg
//
LOCUS       DATITW010000431         1995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_582135, whole genome shotgun sequence.
ACCESSION   DATITW010000431 DATITW010000000
VERSION     DATITW010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582135"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VLS86_06035"
     CDS             <1..404
                     /locus_tag="VLS86_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HSO50087.1"
                     /translation="LLYAYCEATVPRITVITRKAYGGAYLVMNARGIRADLVFAWPSA
                     EITVMGAQGAVNVIHRREITATDDPEPVRSELISDYEERFNNPYVAAELGLVDEVIEP
                     RETRYKLIRALDILRTKRETLPPKKHGNGPQ"
     gene            404..>1995
                     /locus_tag="VLS86_06040"
     CDS             404..>1995
                     /locus_tag="VLS86_06040"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HSO50088.1"
                     /translation="MKSLLVANRGEIAVRVIRTAREMGIKTIAVYSELDRDALHVDLA
                     DEAWNAGAAPAADSYLNQQRILQIAHEAGAEAIHPGYGFLSENAGFAQAVANEGIVWV
                     GPPASAIQAMGDKITSRRNAEQFGVPTVPGITEPITTVEEVAALAEQFGYPVAIKAAH
                     GGGGKGLRVVNGPDELTDSLEGARREADAYFGNPEVYVEKYLEQPRHVEAQILFDTHG
                     KGVFLGERDCSVQRRHQKLIEETPSPGMTPRQRRALGKAALAAARSCEYVNAGTVEFL
                     MDRHGDFYFLEMNTRLQVEHTVTEMVTGIDLVEWQLRIAAGESLTFDTVEARGHAIEF
                     RINAEDPYNNFLPSPGQVVEWQEPAGPGVRVDSWVRAGTTLSQYYDNLLAKLVVWGPD
                     RASAINRGRRALEEFKVRGVASTIPAHLAVLAHPDFNNATHHTRWMEGTVVVNNADPA
                     PSPFPQADDEVERRDITVEIGGRRFSVTYWAPEVGTGAAVRRRPPKLSNAGSAPASDG
                     MVIAPMQGTIVKVHVAAGASVR"
BASE COUNT          382 a          669 c          645 g          299 t
ORIGIN      
        1 agctgctcta cgcctactgc gaggcgaccg tgccccggat cacggtgatc accaggaagg
       61 cctacggcgg cgcgtatctg gtcatgaacg cccgtggcat ccgggccgac ctggtctttg
      121 cctggccctc ggcggagatc acggtgatgg gcgcccaagg tgcggtcaat gtcatccacc
      181 ggcgcgagat aactgcgacc gacgacccgg agccggtgag gtccgagctc atctccgact
      241 acgaggagag gttcaacaac ccctacgtcg ccgccgagct cggtttggtc gacgaggtca
      301 tcgagccccg ggagacccgc tacaagctga ttcgcgcact cgacatcctg cgcaccaagc
      361 gtgagacgct tccgccgaag aagcacggaa acggtcctca atgatgaagt cgcttctcgt
      421 cgccaaccgc ggcgagatcg cagtgcgggt catccgcacc gcccgggaga tggggatcaa
      481 gacgatcgcg gtctattccg agctggaccg ggacgccctg cacgtcgatc tcgccgacga
      541 agcctggaat gccggggcgg cgccggccgc cgacagctac ctcaaccagc agcggatcct
      601 ccagatcgcg catgaagcgg gagcggaggc gatccacccg ggctacgggt tcctctccga
      661 gaacgcgggg ttcgcccagg cagtggccaa cgagggaatc gtctgggtag ggcctccggc
      721 cagtgccatc caggccatgg gtgacaagat cacctctcgc aggaatgcgg agcagttcgg
      781 cgtgcccacc gtccccggga tcaccgagcc gatcaccacc gtcgaagagg ttgcggccct
      841 ggccgagcag ttcggctacc cggtggcgat caaggccgca cacggcggag gaggcaaggg
      901 tcttcgcgtg gtcaacgggc ccgacgagct gaccgactcc ctggaaggcg ccagacggga
      961 ggcggacgcc tacttcggca atcccgaggt gtacgtcgag aagtacctcg aacaaccccg
     1021 gcacgtcgag gcgcagatcc tcttcgacac tcatggcaaa ggggtcttcc tcggcgagcg
     1081 tgactgctcg gtgcaacgac gccaccagaa actgatcgaa gagacacctt cccccggtat
     1141 gacccccagg cagcgacggg cactgggcaa ggcggcactg gccgcggcca ggtcctgcga
     1201 gtacgtaaac gcaggaaccg tcgaattcct gatggatcgt cacggcgact tctacttcct
     1261 cgagatgaac acccgtctcc aggtggagca cacggtcacc gagatggtca ccgggatcga
     1321 cctggtcgaa tggcaactgc gaatcgcggc cggcgagagc ttgaccttcg acaccgtcga
     1381 ggcgaggggg cacgccatcg agtttcggat caacgccgag gacccgtaca acaacttcct
     1441 tccctctcca ggccaggtgg tggaatggca ggagcctgcg ggccccgggg tgcgggtcga
     1501 ctcctgggtc cgggcgggca ccaccctctc ccagtactac gacaacctcc tggccaaact
     1561 cgtggtctgg ggccccgacc gagccagtgc catcaaccgc ggccgaaggg cattggagga
     1621 gttcaaggtg cggggtgtgg cctcgaccat tcccgcccac ctggcggttc tcgcccatcc
     1681 cgacttcaac aacgccaccc accacacgag gtggatggag ggcaccgtcg tcgtcaacaa
     1741 cgccgacccc gctcccagcc ctttccccca ggccgacgat gaggtagagc gccgcgacat
     1801 caccgtcgag atcggtggcc gtcgcttctc agtcacctac tgggcgccgg aggtcggcac
     1861 gggtgccgcg gttcgacgtc ggccaccaaa gctgtcgaat gccggatcgg ccccagcgtc
     1921 cgacggaatg gtgatcgctc cgatgcaggg gacgatcgtc aaggtccacg tcgccgcggg
     1981 cgcatcggtc aggga
//
LOCUS       DATITW010000432         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_341255, whole genome shotgun sequence.
ACCESSION   DATITW010000432 DATITW010000000
VERSION     DATITW010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341255"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VLS86_06045"
     CDS             complement(<1..957)
                     /locus_tag="VLS86_06045"
                     /inference="COORDINATES: protein motif:HMM:NF037526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50089.1"
                     /translation="MADQLQEAMDSLRLIESPSLPILSVYLDLAPERIERRSIGARLR
                     DLLDPIEKVAASGDLDHESSMSLRAGVEHILAMTPVLVTELGSGVAMFVCDGLDLDRH
                     LTMPRGVWDSAVAGPRPYLRPIQAVVDQYRKIATVVLDVRSAEMTVSRMGEVLDREVI
                     EAEELRKSNLAGWHGLEERRHRQHAEEARNHMFREIAERLSRLRRDSGVDLVLVGGQT
                     ETTSALIPFLEPRLQEITQTFVIDLNTLTPSLLATRVSEIEETFEREEEARQVEETYA
                     AAAAGDLAFVGVERVLRAVNRHAVARLLLHDSQSIPGSMCRDC"
     gene            1106..1753
                     /locus_tag="VLS86_06050"
     CDS             1106..1753
                     /locus_tag="VLS86_06050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HSO50090.1"
                     /translation="MKMTPETWQYINEYSREVFGAQDDHLALLMKEATDARLPPIAVG
                     PDVGRLLMILTSMTRGRLAVEVGTLGGYSGIWITRGLQPDGRLITIESDPKHAAFALR
                     QFGLAGVGDRVELRQGQGLEVLAGLSDELEAESVDVLFLDAVKSEYVDYLRIARPLLA
                     HGGLVIADNVYGTGAGWIDQGHGTDGFNRHIAADPGFEAVALPIREGVLVGRRIS"
     gene            complement(1746..>2282)
                     /locus_tag="VLS86_06055"
     CDS             complement(1746..>2282)
                     /locus_tag="VLS86_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50091.1"
                     /translation="GMTETCGQVATLEPGSPVSNRAQPLPGVEIRIETDGRIAVRGPM
                     VSPGYVGQPDREPGEWFVTGDLGEIDDDGALRVTGRADILIVSGGENIDPSIVEATLA
                     RVHGVDSAVVVGVPSEEWGMEAVCLYVGDAGPVRIESHLKGRLPGFMVPKRWLRVDEL
                     PMTAMGKPDRIEAARRFS"
BASE COUNT          399 a          776 c          710 g          397 t
ORIGIN      
        1 acagtcccgg cacatcgacc ccgggatcga ctggctgtcg tggagcagca gacgcgccac
       61 cgcatgacgg ttgacggccc tcaacaccct ctcgaccccg acgaatgcca ggtcaccggc
      121 ggctgcggcc gcataggtct cctcgacctg gcgtgcttcc tcctcacgtt cgaaggtctc
      181 ctcgatctcg gacactctgg tggcgagaag acttggagtc agcgtgttga ggtcgatcac
      241 gaaggtctgg gttatctcct gcagccgtgg ctcgagaaac gggatcagcg cactggtcgt
      301 ctccgtctgc ccacccacca ggaccaggtc gaccccggaa tcgcgacgaa gccgactcag
      361 acgctcggcg atctcgcgga acatgtggtt gcgagcctcc tcggcgtgct gacgatgccg
      421 ccgctcctcc agaccgtgcc agccggcgag attggacttc cgcaactctt cggcctcgat
      481 gacttcccgg tcgagtacct cccccatgcg ggaaaccgtc atctcggcgc ttctgacgtc
      541 gaggacgacg gtggcgatct tccggtactg gtcgacgacg gcctggatcg gccgcaaata
      601 gggtcgtggc ccggcaacgg ctgaatccca aaccccgcga ggcatggtca gatgccggtc
      661 cagatcgagc ccgtcgcaca cgaacatggc gacacccgag ccgagctccg tgaccaggac
      721 gggtgtcatc gccaggatgt gttcgacgcc ggcgcgcagc gacatcgagg attcatgatc
      781 caggtcgccg gacgcggcca ccttctcgat cggatcgagc aagtcccgaa gacgggcgcc
      841 gatcgatctt ctttcgatcc gctccggtgc caggtcgaga tacaccgaca ggatcggcag
      901 cgatgggctc tcgatgagcc tcaggctgtc catcgcctct tgcagctgat cggccacttc
      961 ctaccttctt tcgcggcgaa ccggccccga gacgggcttc aatcatcacg ctatgaccag
     1021 ctcccgggct ggggaagggc cgttggtcac aagtcgacag ctccaggttt cggaggctca
     1081 agaccaatga ccagaatggt tggacatgaa gatgaccccc gagacctggc agtacatcaa
     1141 cgaatactcc cgtgaggtgt tcggcgccca ggacgaccat ctggccctgc tgatgaagga
     1201 ggcaacagat gcccggctgc cgccgatcgc ggtcggcccg gacgtaggcc gattgctgat
     1261 gatcctcacc tcgatgacca ggggacgact tgccgtcgaa gtcgggaccc tcggcgggta
     1321 ctccggaatc tggatcacgc gcggccttca acccgatggc cggctcatca ccatcgagag
     1381 cgacccgaag catgctgcct tcgcccttcg tcagttcggg cttgccggtg tcggcgaccg
     1441 ggtggagctt cgccagggac aggggctgga ggtcctggcc gggctgtcgg acgagctcga
     1501 ggccgaaagc gtcgacgtcc tctttctcga cgcggtcaag agtgaatatg tcgactacct
     1561 gcggattgcc cgcccgctgc tcgcgcacgg cggtctggtc atcgcggaca atgtctacgg
     1621 gaccggggcc ggttggatcg atcaaggcca cggcaccgac gggttcaacc gacatatcgc
     1681 cgccgacccc gggttcgagg cagtggcgct gccgatccgg gaaggggtcc tcgtcgggcg
     1741 tcggatcagc tgaaacgacg agccgcttcg atgcggtccg gcttgcccat ggctgtcatc
     1801 ggcagctcgt ccacccgcaa ccagcgtttg gggaccatga acccgggcag tctgcccttg
     1861 agatgggact cgattcggac cgggcccgca tcgccgacgt agagacacac ggcctccatc
     1921 ccccattcct cgctgggcac cccgacgacc acggccgagt cgacaccgtg gacccgtgcc
     1981 aatgtggctt ccacaatgct cgggtcgatg ttctccccgc cactgacgat caggatgtcg
     2041 gcgcgacccg tcacccgaag cgctccgtcg tcgtcgatct cccccaggtc accggtcacg
     2101 aaccactcgc ccggctcacg gtcgggctgg ccgacatagc ccggcgacac catgggccct
     2161 ctgaccgcga tccgcccgtc agtctcgatt cggatctcga cgccggggag cggttgggca
     2221 cggttgctca ccggagatcc tggctcgagc gtcgccacct ggccgcaagt ctcggtcatg
     2281 cc
//
LOCUS       DATITW010000433         1743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_381398, whole genome shotgun sequence.
ACCESSION   DATITW010000433 DATITW010000000
VERSION     DATITW010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381398"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VLS86_06060"
     CDS             <1..521
                     /locus_tag="VLS86_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSO50092.1"
                     /translation="WGDAIAKTKIGLSFSEPTASISLISPDNYREFIAPYHKELVDHF
                     KAQKVGVTVHICGTTYPIYEDLIGCGFSTITFDLDQQGDPKLYVDQLTRFMEVARGRA
                     VAIGNVDATKFEKTTKAEMEADVRRCVDAAARHSSFILSTSCEIPPRSSPEIVQWFMD
                     AAHDYGRYDRLL"
     gene            complement(551..958)
                     /locus_tag="VLS86_06065"
     CDS             complement(551..958)
                     /locus_tag="VLS86_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSO50093.1"
                     /translation="MASKLYVGGLSYSTTGETLREYFAQCGTVESASVITDKFSGQSR
                     GFGFIEMSTAEEAQRAISELNGKELDGRKITVNVSNPRTPGSGGGGRGPGGPRGGGGG
                     GGGRPGGGRGGGGFGGGDRGAYGVPPSKKPFRW"
     gene            complement(1121..1342)
                     /locus_tag="VLS86_06070"
     CDS             complement(1121..1342)
                     /locus_tag="VLS86_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50094.1"
                     /translation="MRVRTRTRARALAAYALVLAVVLGVLGLLVMTSESRHPAPLTPA
                     AEPEKIEVAAPEDTELSPVERAMGEMRTR"
     gene            complement(1333..>1743)
                     /locus_tag="VLS86_06075"
     CDS             complement(1333..>1743)
                     /locus_tag="VLS86_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSO50095.1"
                     /translation="TMMHVLLIEVNRAFAESVAMSCITDGIAVRLAETLCEGVRYMMD
                     GPVSVVLIDSALMRLSGREQVRLFDAVAPGVPVAVLVPEGTTVEETVKRQLQGFQVVA
                     KPFEVRDLLTTVKRPARPSRARPGATAQIEALCG"
BASE COUNT          323 a          619 c          527 g          274 t
ORIGIN      
        1 tctggggcga tgccatcgcg aagaccaaga tcgggctcag cttctccgag cccaccgcct
       61 ccatcagtct catctccccg gacaactatc gcgagttcat cgcgccctat cacaaggagc
      121 tggtcgacca tttcaaggcc caaaaggtgg gtgtgacggt gcacatctgc ggcaccacgt
      181 atccgatcta cgaggacctg atcggctgcg gtttctcgac catcaccttc gacctcgacc
      241 agcaggggga tccgaagctg tacgtcgacc agctcacgcg cttcatggag gtcgccaggg
      301 ggcgcgccgt cgcaatcggc aacgtcgacg ccacgaagtt cgagaagacc accaaggcgg
      361 agatggaagc ggacgtgcgg cgctgcgtgg acgcggcagc ccggcactcg agcttcatcc
      421 tgtcgacgtc ctgcgagatc ccgccgcgat ccagccccga gatcgtgcag tggttcatgg
      481 acgcggccca cgactatggc cgctacgaca gactgctatg agggggccgt cgagaccccc
      541 tcatagcgtc tcaccagcgg aacggcttct tcgagggagg gacgccgtac gcgccccgat
      601 caccgccacc gaacccaccg cctccccgac caccgccggg tcggccacca ccacccccgc
      661 cgccaccgcg cgggccaccg gggccgcgcc caccgccgcc tgaaccaggc gtgcgcgggt
      721 tggagacgtt gacggtgatc ttccgaccgt cgagctcctt gccgtttagc tcggagatcg
      781 cccgctgggc ctcctcggct gtcgacatct cgatgaagcc gaagccacgg gattggccgg
      841 agaacttatc ggtgatcacc gaggcggatt cgacggtgcc gcattgggcg aagtactcgc
      901 gcagggtctc tccggtcgtg gagtaggaca aaccacccac atagagcttc gatgccatgc
      961 ggttctcctt agcagttgct cttccggtcc cgggatgcgc cccaatactc ggaagagtca
     1021 atatcggagg cgagagcgcc ggccacgacg gctcggccta gagtctacac catgaccgag
     1081 agtcgtgcca cggacgcccg ggcatcacgg gcatcgagtg tcagcgtgtt cgcatctcgc
     1141 ccatcgcgcg ctcgaccgga ctcagttcgg tgtcttcggg cgcagccact tcgatcttct
     1201 cgggctcggc ggcaggtgtc agcggtgccg gatgtcgact ctcgctggtc atgaccaaga
     1261 gaccgagcac acccagcacc acggccagca ccagggcata ggccgcaagc gcccgagccc
     1321 gggttctggt ccttacccgc acagcgcctc gatctgcgcg gtggcgcccg ggcgcgcccg
     1381 agacggccgc gcgggccgct ttaccgtggt caggaggtcg cggacctcga acggcttggc
     1441 gaccacctgg aatccttgca gctgacgctt caccgtctcc tcgaccgtcg tcccctccgg
     1501 tacgagcacc gccaccggta cgcccggcgc gacggcgtcg aagagccgaa cctgctcgcg
     1561 acccgacaac cgcatcaggg ccgaatcgat caagacgaca gagaccggcc cgtccatcat
     1621 gtaccgaacc ccctcgcaca gcgtctccgc cagccgaacg gcgatgccgt cggtgatgca
     1681 ggacatcgcc accgactcgg caaacgcccg attaacctcg atcaaaagga catgcatcat
     1741 agt
//
LOCUS       DATITW010000434         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_461681, whole genome shotgun sequence.
ACCESSION   DATITW010000434 DATITW010000000
VERSION     DATITW010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461681"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VLS86_06080"
     CDS             complement(<1..830)
                     /locus_tag="VLS86_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSO50096.1"
                     /translation="MTLDQLGAAVGRPAPYLSLLENGKKEPRINLVLELASALDIDIA
                     QLLDPSPPNRRDTLEIALLKSQESALFDSLDLPTIKPSARMDNETLTHLVGIHEALRE
                     RSNLTSAGSEEVRRANGAVSTWIAGRDGYLEEVEIEARQALDSSGYSGEGPFSSRNLL
                     DLARHAGFEIHPIDDMPTFARSVIDLDQRRVYIAQRDELRTRQARKAVLQTLAGFLLG
                     HEPQPDLDTFLRQRVETAYFASAVLAPEDAVVARLSTAKEQHDIDVEDVKELFYLSYE
                     "
     gene            986..2545
                     /gene="aceB"
                     /locus_tag="VLS86_06085"
     CDS             986..2545
                     /gene="aceB"
                     /locus_tag="VLS86_06085"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HSO50097.1"
                     /translation="MNQFQATEIERRILPSEAVVFLTDLEREFGWRRGQILDARQTRQ
                     DRIDAGEMPGFLESTAEIRAAEWRIAPVPPDLEDRRVEITGPVDAKMMINALNSGARV
                     FMADFEDATTPTWSNLLAGQANLVEAYRRELQFETDAKTYRLNEDIATLVVRPRGWHL
                     PEKHFLVDGQPMSASLFDFGLYLFHGGRAAIEAGSGPYLYLPKLESHVEARLWNDVFV
                     WAQDRLGLAEGTIKATVLIENILAAFEMDEILFELREHAAGLNAGRWDYIFSMIKKFR
                     NNPQFVLPDRSQVTMTVPFMRAYTELMVKTCHRRGAHALGGMAAFIPNRRDPEVTESA
                     ITAVTADKAREAGAGCDGTWVAHPDLVPVAMAQFDAVLGDRPNQVSRQRGDVSVTARD
                     LLDLEFPGTVTRAGIETNVSVGIQYIASWLSGVGAAGINNLMEDVATAEISRSQIWQW
                     VRHGVTAEDGTAITESLVRSIAEGVVERLVAEDHPHTEQLHNARKLFEEVALGEHFVD
                     FLTLPAYDLVN"
BASE COUNT          452 a          847 c          837 g          465 t
ORIGIN      
        1 atctcgtatg agaggtagaa cagctccttg acatcctcga cgtcgatgtc gtgctgctcc
       61 ttggcggtgc tcagccgggc aacgaccgcg tcctcgggtg ccagcaccgc agatgcgaag
      121 taggcggtct ccacccgctg acgaagaaac gtgtcgaggt ccggctgtgg ctcgtgcccg
      181 agtaggaacc cggccagagt ctggagaacg gcctttcgcg cctggcgggt gcggagctcg
      241 tcgcgctggg cgatgtacac cctgcgctgg tcgagatcga tcaccgaccg ggcgaaggtg
      301 ggcatgtcgt cgatcgggtg gatctcgaat ccggcgtgtc gggccaggtc gagcaggttg
      361 cgcgaggaga atggtccctc acccgagtag ccactcgagt cgagcgcctg tctcgcctcg
      421 atctcgacct cctccaggta gccgtcgcgc ccggcgatcc aggtgctgac cgctccgttg
      481 gcccgtcgca cctcctcgga tcccgccgaa gtcaggttgg accgctcccg cagtgcctca
      541 tggatgccga ccaggtgagt cagtgtctcg ttgtccatcc tggcgctcgg cttgatcgtg
      601 gggaggtcga gcgagtcgaa tagagcgctt tcctgtgact tgagcagggc gatctccagg
      661 gtgtcccggc ggttgggtgg gctggggtcg agcagctggg cgatgtcgat gtccagggcc
      721 gaggccagct ccagcaccag gttgatcctg ggctccttct tcccgttctc cagcagcgag
      781 aggtatggcg ccggtcttcc caccgcggcg ccgagctgat ccaatgtcat tccccgctct
      841 cttcggtgat gacggagacg acgacccagg accagtggat cgagatgctg aggttgggtc
      901 aaatcaaccg tccttcgaaa gatcgtgcga tattatgcca gagactgctc ccactatagc
      961 cgcaggtcta agaagctgat ctcttatgaa ccagttccag gcaaccgaga tcgagcgccg
     1021 catcctcccc tccgaagcgg tggtattcct caccgatctc gagcgtgagt tcggctggcg
     1081 aaggggccag atcctcgacg cccggcagac gcgtcaggat cggatcgacg ccggtgagat
     1141 gccgggcttt ctcgagtcga ccgcagagat cagggccgcg gagtggcgga tcgccccggt
     1201 tccccccgac ctggaggacc gcagggtcga gatcaccggc ccggtcgacg ccaagatgat
     1261 gatcaatgcc ctcaactcgg gggcgcgggt cttcatggcc gacttcgagg acgccaccac
     1321 accgacctgg tcgaatctcc tcgccggtca ggcaaatctg gtcgaggcct atcgccgtga
     1381 gctccagttc gaaaccgatg ccaagaccta tcggctgaat gaggacatcg caaccctcgt
     1441 ggtcagaccc cgcggctggc atctccccga gaagcacttc ctggtcgatg gccagccgat
     1501 gtcagcgtcg ttgttcgact tcggcctgta cctgttccac ggtggcaggg cggcgatcga
     1561 ggccgggagc gggccgtacc tttaccttcc caagctggag tcccacgtcg aggcgaggct
     1621 gtggaatgac gtgttcgtct gggctcagga tcgtctcggc ctcgccgagg ggacgatcaa
     1681 ggccacggtc ctcatcgaga acatcctcgc cgcgttcgag atggatgaga tcctcttcga
     1741 gttgcgtgag catgccgccg ggctcaacgc cgggcgctgg gactacatct tctcgatgat
     1801 caagaagttc cggaacaacc cccagttcgt gctcccggac cgctcccagg tgacgatgac
     1861 cgtgcccttc atgcgggcct acaccgagct catggtcaag acctgtcacc ggcggggcgc
     1921 tcacgccctc gggggcatgg cggcgttcat ccccaaccgg cgggacccgg aggtgaccga
     1981 gtccgcgatc accgcggtga ccgcggacaa ggcgcgggaa gccggcgccg gctgcgacgg
     2041 gacctgggtt gcccaccccg acctggtccc ggtggcgatg gcacagttcg acgcggtgct
     2101 cggggaccgg cccaaccagg tcagccgtca acgcggggac gtgtccgtca ccgcccgtga
     2161 tctcctcgac ctggagttcc ccgggaccgt cacccgcgcc ggcatcgaga ccaatgtctc
     2221 ggtcggcatt cagtacatcg cctcctggct ctcgggcgtc ggggccgcag gcatcaacaa
     2281 cctgatggag gacgttgcca ccgccgagat ctcgagatcc cagatctggc agtgggtgag
     2341 acacggtgtc acggccgaag acggcaccgc gataaccgag tccttggtgc gctcgatcgc
     2401 cgaaggcgtc gtcgagcgac tggtggccga ggaccaccca cacaccgagc agctgcacaa
     2461 cgcccgcaag ctgttcgagg aggttgccct cggagagcac ttcgtggact tcctcaccct
     2521 tcccgcctac gacctcgtca actgaggaga aacaccatgt ccgaccgcgc ccatgaggca
     2581 atccgtctcg ccgacgaatg g
//
LOCUS       DATITW010000435         1730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_661736, whole genome shotgun sequence.
ACCESSION   DATITW010000435 DATITW010000000
VERSION     DATITW010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661736"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..931)
                     /locus_tag="VLS86_06090"
     CDS             complement(8..931)
                     /locus_tag="VLS86_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSO50098.1"
                     /translation="MTATKQEVSIVTARVNEIEVELVAGSTVLDACRAVGADIPTLCH
                     GPTMTAANACRVCVVEVEGSRALVPSCSRTLDDGMEINTESDRALHSRRLVMELLGSA
                     SELDHAGDRLATWMAAAGAAPTRFGPAEPETPRDRPLPGHHHAPRTDISATVAEPVKI
                     EDDLYVRDYSRCILCYRCVDACGVEHQNTFAIAVAGRGFDARISTEFDVALPDSACVY
                     CGNCIAVCPTGALVGKIEFDMRAAGTWDETRQEVTRTVCSYCGVGCNLDLHTQDGRIV
                     NVTSPHDHDVTLGNLCVKGRFGWQYVSSGND"
     gene            complement(928..>1730)
                     /locus_tag="VLS86_06095"
     CDS             complement(928..>1730)
                     /locus_tag="VLS86_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HSO50099.1"
                     /translation="GDDVTGAGFGFDIELRRGAGAYICGEETALFASIEGHRGEPRQK
                     PPFPTENGLFGKPTSVNNIETLVAVLAVLELGAEGFAAIGTERSAGPKLFCISGSVGR
                     PGLYEVPFGTTLRDLITLAGGVRDGLGLGAVLVGGAAGTFVDAGSLDMELTFEGAAAN
                     STSLGSGAVIVFDDQTDFAPILRRIAQFFRDESCGQCVPCRIGTVRQEEALARLAADR
                     PLGSRAEEMQRLDDLAQVMADASICGLGQTAAAAVQSAFRLGLPGVAP"
BASE COUNT          282 a          585 c          582 g          281 t
ORIGIN      
        1 gaccgcctca gtcatttcca ctgctgacgt attgccaacc gaagcgcccc ttgacgcaga
       61 ggttgcccag tgtcacatcg tggtcgtggg gtgaggtcac attgacgatc cggccgtcct
      121 gggtgtggag gtcgaggttg cacccgacac cgcagtagga gcaaacggtg cgtgtcactt
      181 cctgacgcgt ctcgtcccag gtccctgcag ccctcatgtc gaactcgatc ttgccgacga
      241 gggcgcctgt gggacagacg gcgatgcagt tgccgcaata gacgcaggcc gagtcgggaa
      301 gtgcgacgtc gaactcggtg gagatcctgg cgtcgaaccc tctcccggcc accgcgatgg
      361 cgaatgtgtt ctgatgctcc accccgcagg catcgacgca gcgatagcac aggatgcagc
      421 gggaatagtc ccggacgtag agatcgtcct cgatcttgac cggttcggcg accgtggccg
      481 agatgtccgt tcgcggcgcg tggtgatggc cgggcaaggg ccggtcgcgt ggggtctccg
      541 gctctgctgg cccgaagcgg gtcggtgccg cgccggccgc tgccatccag gtcgcaagcc
      601 ggtctccggc gtggtcgagc tccgaagccg acccgaggag ctccatcacc agtcgccggc
      661 tgtggagggc ccgatccgac tcggtgttga tctccatccc gtcgtcgagg gtgcgagagc
      721 acgacgggac cagagcccgg gagccttcca cctcgactac gcacacccgg caggcgttgg
      781 ccgcagtcat cgtcgggccg tgacacaggg tcgggatgtc ggcgccaact gcccggcagg
      841 cgtcgaggac ggtcgagccg gcaaccagct cgacttcgat ctcgttgacc cgggcggtca
      901 cgatcgacac ctcctgcttc gtggcggtca tggggccacc ccgggaagac cgagccgaaa
      961 cgccgattgc actgcggcgg ccgcggtctg tcccagcccg cagatcgagg cgtctgccat
     1021 cacctgcgcc aggtcgtcga gccgttgcat ctcctcggct ctgctcccca gtggccgatc
     1081 ggccgcgagc cgcgccagcg cctcctcctg acggacagtc ccgatccggc aggggacaca
     1141 ttgcccgcac gactcgtcac gaaagaactg tgcgatcctg cgcaggatcg gagcgaagtc
     1201 ggtctggtcg tcgaagacga tgactgcccc cgacccgagg ctcgtgctgt tcgcggccgc
     1261 gccctcgaag gtgagctcca tgtcgagcga gcctgcgtcg acgaaggtcc ccgccgctcc
     1321 tcccaccagg accgccccga ggccgagacc gtccctcact ccgccggcca gggtgatcag
     1381 gtcgcggagg gtggtgccga aggggacttc atacagcccg ggtctgccca ccgagccgga
     1441 gatgcagaaa agcttgggcc cggcggagcg ctcggtgccg atggcggcga acccctcagc
     1501 gcccaattcg aggacggcga ggactgcgac cagggtctcg atgttgttga ccgaggtcgg
     1561 tttgccgaac agcccatttt cggtggggaa cggcggtttc tgtcgcggct caccgcgatg
     1621 accctcgatc gaggcgaaaa gggcggtctc ctcgccacag atgtaggccc cggccccacg
     1681 tcggagctcg atgtcgaagc cgaacccggc cccggtcacg tcgtcgccga
//
LOCUS       DATITW010000436         2835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_582276, whole genome shotgun sequence.
ACCESSION   DATITW010000436 DATITW010000000
VERSION     DATITW010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582276"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VLS86_06100"
     CDS             complement(<1..218)
                     /locus_tag="VLS86_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50100.1"
                     /translation="MIDDVNESQVPTDPDPVNSGGAGEESSATTAGTGAAVSQGTADA
                     DDAPAAVTEVLSPVATATLPPPAPESPG"
     gene            complement(261..1415)
                     /locus_tag="VLS86_06105"
     CDS             complement(261..1415)
                     /locus_tag="VLS86_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSO50101.1"
                     /translation="MPAAWRSIRFRLSVQYSAIVFGLGGALLGLVYMAMRRWLRSQTM
                     TQYVWTGRPVFQDGRQIGVVPSIEAHEIRMIESVYNEIVLNEVARFTIFALIALFLLS
                     LVTGWVMSGRVLKPVEEITDVAREIQASDLSRRIVLDGPDDEITRLASTFNGMLERLD
                     RAFSSQRRFLADTSHDLRTPLAVIRSNIEVVADDDNAKVEDWQEVGGIVRRNVEKMSE
                     MIDGLLATARLQTGKAQAVALNLATLVESKAAELETLVSEQGILLETRTAPALVEGVE
                     VSLDRAFTNLLDNAIRVSSPGSTIRVGVGVVEGWAWMAVADEGPGMPAEPSGGRFGLG
                     LSIVEQIAEAHDGALVAHEGPDGLGTTMVIWLPTPGAKTPRPKMVPFTDA"
     gene            complement(1415..2089)
                     /locus_tag="VLS86_06110"
     CDS             complement(1415..2089)
                     /locus_tag="VLS86_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020679123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSO50102.1"
                     /translation="MRLLVVEDEPDLANAIARGLRRDGHAVDTALTAGEADIKLRTAG
                     YDLVCLDWNLPDGSGITMCRELVSGALPTLEGDRPRILMLTARDGVEDRVAGLDSGAD
                     DYLVKPFAFAELSARVRALLRRDDQTDPVIETGPLRLDPARFEATRDGRPLQLTAKEF
                     ALLHYFMANAGRVLSQEHLLEHVWDEMADPLTNVVRVTVSNLRKKLGDPPLIKTVSGR
                     GYRLGD"
     gene            2251..2424
                     /locus_tag="VLS86_06115"
     CDS             2251..2424
                     /locus_tag="VLS86_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50103.1"
                     /translation="MVVTFITQDQDGFHTYTGHVISRGPATIFVMLTEPFSMAGRSVQ
                     FPITQVIEERVVV"
     gene            2476..2739
                     /locus_tag="VLS86_06120"
     CDS             2476..2739
                     /locus_tag="VLS86_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50104.1"
                     /translation="MFLAASTWQALAIAGGVGALAGLIGGFFASADNLFGAALIGIIG
                     GIAGSAIMRIAGAPSFISVGDDFSLVWAAAGGLLLGYVVGRSN"
BASE COUNT          497 a          930 c          886 g          522 t
ORIGIN      
        1 atccccggcg attcgggtgc cggtggcggc aaagttgcag tggcgaccgg ggacaggacc
       61 tcggtgaccg cggcgggggc gtcgtccgcg tcggcggtgc cctggctgac tgccgccccg
      121 gtcccggcgg tcgtggcgga cgattcctcg cccgccccgc ccgaattcac cgggtcggga
      181 tctgtcggaa cttgagactc gtttacgtca tcgatcatcg atgtggacct cctggcactg
      241 tccaccctac gaaccgagga tcaagcatct gtaaatggaa ccattttcgg gcggggagtc
      301 ttggcgccgg gtgtggggag ccagatcacc atcgtcgtcc ccaggccgtc aggcccttcg
      361 tgggcgacga gggccccatc gtgcgcctcg gctatctgct ccacgatcga gaggccgaga
      421 ccgaaccgac cccccgacgg ctccgcaggc atgcccgggc cctcatcggc gaccgccatc
      481 caggcccacc cctccaccac tccgacaccg acccggatcg tcgaccccgg tgatgagacc
      541 cttatcgcat tgtcgagcag gttggtgaac gcccgatcca gggagacttc gactccctcc
      601 accagagctg gggcagtccg ggtctcgagg aggatcccct gctcggagac cagtgtctcc
      661 agctcggcgg ccttggactc gaccaatgtg gccagattca atgccacggc ctgcgccttt
      721 ccggtctgaa gtcgcgccgt ggccagcaga ccgtcgatca tctcggacat cttctcgacg
      781 ttgcgccgga cgatgccccc cacctcttgc caatcctcga ccttggcatt gtcgtcgtcg
      841 gcgaccacct cgatgttgga tcggatgacc gccagcgggg tccgcagatc gtgactggtg
      901 tcagccagga atcgacgctg actggagaac gccctgtcca ggcgctccag catcccgttg
      961 aacgtcgagg cgagacgggt gatctcgtcg tccgggccgt cgaggacgat ccgccggctc
     1021 agatccgagg cctggatctc tctcgccaca tcggtgatct cctccaccgg cttgagcacc
     1081 cggcccgaca tcacccagcc ggtgacgagg ctgagcagga agagggcaat cagggcgaag
     1141 atggtgaatc tcgccacttc gttgaggacg atctcgttgt agaccgactc gatcatccgg
     1201 atctcgtgag cctcgatgct ggggaccacc ccgatctggc gaccatcctg gaacacggga
     1261 cggccggtcc agacgtattg ggtcatggtc tgggagcgga gccagcgacg catcgccata
     1321 tagaccaggc cgagcagggc cccacccaac ccgaagacga tcgccgagta ctgcaccgac
     1381 aggcggaagc gaatcgagcg ccaggccgca ggcatcagtc tcccaaccgg tagccccgtc
     1441 cggagacggt cttgatcagg ggtgggtccc cgagtttctt gcggagattg ctgaccgtca
     1501 cccgcaccac attggtgagc gggtcggcca tctcatccca gacatgctcc agcaagtgct
     1561 cctgactgag cacccgcccg gcattggcca tgaaatagtg gaggagagcg aattccttgg
     1621 ccgtcaactg cagagggcgc ccatccctgg tggcttcgaa tcgagccggg tcgaggcgca
     1681 gcggcccggt ttcgatgacg gggtcggttt gatcgtcccg ccgcagaagg gctcgcaccc
     1741 tggccgagag ctcggcgaag gcgaacggct tgaccaggta atcgtcggcg ccggagtcga
     1801 gaccggccac ccgatcctcg actccgtcac gagcggtgag catcaggatc cgcgggcggt
     1861 ctccctccag ggtgggcagg gcgccgctga ccaactcgcg gcacatggtg atccccgagc
     1921 cgtcggggag attccagtcg agacacacca agtcgtatcc ggcggtccgg agcttgatgt
     1981 cggcctcgcc ggccgtgaga gcggtgtcga cagcatgccc gtcacgacga aggcccctgg
     2041 cgatggcatt ggccagatca ggttcgtctt cgactactaa gaggcgcacg tcccatcgaa
     2101 tttatgcttg atttcgtaac ttgtggctaa acccgggcga cggaccggcg cggccaggat
     2161 gggtcttaag accctaaggg acattcggtc accagccgac tcaatgatcg tgcaaccaag
     2221 tgccgatgac atgagtcgga gtgttcagca atggtcgtca ccttcataac ccaggatcaa
     2281 gacggattcc acacctacac aggccatgtg atcagtcgcg gcccggcaac catcttcgtg
     2341 atgctcaccg agccgttctc catggccggc cgctcggtgc agttcccgat cacccaggtg
     2401 atcgaggagc gagtcgttgt gtgatgggtc ggtagatcca tcgattcgga cctggtcccg
     2461 gcttagtctg tcccgatgtt cctagcagcg tccacgtggc aggcactcgc catcgccggt
     2521 ggcgttggag ccctggccgg tctcatcggc ggcttcttcg ccagtgccga caatctcttc
     2581 ggtgcggccc tgatcggaat catcggaggc atcgccggat cggcgatcat gagaatcgcc
     2641 ggtgcaccgt cgttcatctc ggttggcgac gacttctccc tggtctgggc ggccgccggg
     2701 ggcctcctcc tcggctacgt cgtcggtcgc tccaactgat cccagcttcc ccagtttgag
     2761 tccctttccc tccgggtaaa tgtctcgtgt accgggagac tcccgggcga gatttcacaa
     2821 aggagatttc gatgg
//
LOCUS       DATITW010000437         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_622196, whole genome shotgun sequence.
ACCESSION   DATITW010000437 DATITW010000000
VERSION     DATITW010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622196"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /gene="glmS"
                     /locus_tag="VLS86_06125"
     CDS             complement(<1..1420)
                     /gene="glmS"
                     /locus_tag="VLS86_06125"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HSO50105.1"
                     /translation="MCGIVGYVGPKPVIDVLMPGLARLEYRGYDSAGVALNHGGSLTV
                     LKQAGRIADLQGLVDREGLSEAHTGIGHTRWATHGAPNTGNAHPHLDCNRDVVVVHNG
                     IIENWIDLKSKLIENGHEFESDTDTEVVAHMIEEMADLPLAEAVRSVMNQADGALALV
                     VMRKSEPDVLVGARRGSPLVVGRGKGENFLASDIPAFLEHTRHMVIVEDDRVVEIRPD
                     EVILTDLGGNSMPLDERLVEWDFEAAEKGGFDTFMLKEIFEQPHAITDTLAGRFEFPG
                     RVVLDELTIDPEILREIDKVFVVACGTSYHAAMMAKYAIERWARLPVEIDIASEFRYR
                     DPVLDDRSLVIGISQSGETIDTLAAVRYAKAQGSVVIGVSNVVESALARESDAVLYTR
                     AGPEIGVAATKTFTTQLVAMQLLGLYLAQVRGALDPGAIEVQVKELLRLPDQVEAVLA
                     RAEELRVMAEPWSDTRDWFFLGR"
BASE COUNT          282 a          547 c          494 g          301 t
ORIGIN      
        1 accgacccag gaagaaccag tcccgcgtat cggaccaggg ctccgccatc accctcaact
       61 cctcggctcg ggcgaggacc gcctcgacct ggtcggggag acggagcagc tccttgacct
      121 gtacctcgat ggccccgggg tcgagggcac cccgcacctg ggccaggtac agaccgagca
      181 gctgcatggc cacgagctgg gtggtgaagg tcttggtcgc cgccaccccg atctcggggc
      241 cagccctcgt gtagaggacg gcgtccgatt cccgggccag ggcggactcg accacattcg
      301 acaccccgat cacgaccgaa ccctgtgcct tggcgtaacg gaccgctgcc agcgtgtcga
      361 tggtctctcc cgattggctg atcccgatca cgagcgaccg gtcgtcgagc accggatcgc
      421 ggtatctgaa ctcggatgcg atgtcgatct ccaccgggag acgggcccac cgctcgatgg
      481 cgtacttggc catcatcgcc gcgtggtagg aggtaccaca ggccacgacg aacaccttgt
      541 cgatctcgcg caggatctcc gggtcgatgg tcaactcgtc gagcaccacc cggcccggaa
      601 actcgaaccg accggcgagc gtgtcggtga tcgcgtgcgg ctgctcgaag atctccttga
      661 gcatgaaggt gtcgaaacca cccttctccg ccgcctcgaa gtcccactcg acgagacgct
      721 cgtcgagcgg catgctgttg ccgcccaggt cggtcaggat cacctcgtca ggtcggatct
      781 cgaccaccct gtcgtcctcc acgatcacca tgtgacgggt gtgctcgagg aaggcgggga
      841 tgtccgaagc caggaagttc tcacccttgc cccggcccac caccagcggc gacccgcgcc
      901 ttgcccctac cagcacatcg ggctccgact tccgcatgac gacaagggcg agggccccgt
      961 cggcctggtt catgacgctg cgcactgcct cggccagggg aaggtcggcc atctcctcga
     1021 tcatgtgagc gaccacctcg gtgtcggtgt cgctctcgaa ctcgtggccg ttctcgatga
     1081 gctttgactt gagatcgatc cagttctcga tgatcccatt gtggacaacc accacatccc
     1141 ggttgcagtc gagatggggg tgtgcgtttc cggtgttcgg cgcgccatgg gtggcccaac
     1201 gggtgtggcc tatcccggtg tgtgcctcgg acagaccctc gcgatcgacc agaccctgca
     1261 aatctgcgat gcgacccgcc tgcttgagga cggtcagcga cccgccgtgg ttgagagcaa
     1321 cacccgccga gtcgtaccct cgatactcca gccgggccag acccggcatc aagacgtcga
     1381 tcaccggctt cggcccgacg tatccaacga ttccgcacat atcaagctcc tgaattcgta
     1441 cctgaggtac atgggctcac atgtgggcct atggggacag agaaacgggg tcgatcggac
     1501 cctcgcgttt ctttgtcgtc gtaggcccgt atttgggctt acgtacctca ggtaagcgtt
     1561 aagcgatgcg tgcggcccac gggtgggtct ctgttccgga atcgcttccg ggttttgctc
     1621 ccac
//
LOCUS       DATITW010000438         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_661847, whole genome shotgun sequence.
ACCESSION   DATITW010000438 DATITW010000000
VERSION     DATITW010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661847"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VLS86_06130"
     CDS             complement(<1..547)
                     /locus_tag="VLS86_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50106.1"
                     /translation="MTAPPIADEKESTTPITPELERAAIKAEARRGFFISLPAFIYLV
                     LLFVFPLLLVFVYSFATRDSTGLTVLEDWNLDSYKRLFSADVLQIFVRSFVLASVTTA
                     LCLLLSYPFAYYIATRSGGSRNLLLVLVMIPFWSNFLVRTYAWRVILGSEGPISQLSV
                     ALGGEPIRFLFTNTAVLIGLVY"
     gene            complement(547..1623)
                     /locus_tag="VLS86_06135"
     CDS             complement(547..1623)
                     /locus_tag="VLS86_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50107.1"
                     /translation="MSKPAVELADVTKRFGDEVVAVNRIDLTVNDGEFFSLLGPSGCG
                     KTTTLRMIAGFEFPTEGSIRVHGAEMGLKPPNQRPINTVFQSYALFPHMTVAENVAFG
                     LNMRKVEKSEVASRVTRVLDQVQLSKRADAKPSQLSGGMQQRVALARALVNEPEVLLL
                     DEPLGALDLKLRQAMQVELKDLQGRVGITFIYVTHDQQEALTMSDRIGVMSEGSLLQV
                     GKSEEIYERPTSRFVADFIGDINLIGATMDGSNSARLSNGLEIGVPAASTPSGTEVTL
                     ALRPERLALYDLDEEIPPGHNRVKVRVMRRLYYGDVYFYDVDGGIGTEIEVKEENRPG
                     VDLYDVGEEAYLVWSPEAANLVLS"
     gene            complement(1623..>2752)
                     /locus_tag="VLS86_06140"
     CDS             complement(1623..>2752)
                     /locus_tag="VLS86_06140"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSO50108.1"
                     /translation="PDPADGRIERTPDGRPQGTLHEGAAHLVERLAPRDTPEEMRLGV
                     LAAQRYLLSKGITAWQDAHVDLATHQAYRSLAGSGDLVCSAVGAMWWDRGLGMEQIDA
                     LEQMRSEPEGRYRSVAVKLMLDGVVENHTAALLEPYLDGHGQETENRGIDFIDPAELK
                     EIVTELDRRGFSCHFHAIGDAAVRNGLDAVEAARLGNGWTGARHHISHIQVVHPDDIP
                     RFRRLGVVANAQALWAQDGIDQIDLTKPYLGEQRSVWQYPFGSLLRAGATLAMGSDWG
                     VSTADVMDQIDTAVTRLNHEAPDLPPLNTGERISFLDGLAGFTAGSAYVNHRDLVSGT
                     LAVGKLADLVILDRDPMAGGRIREASVAMTVVDGEVAFEED"
BASE COUNT          492 a          898 c          894 g          468 t
ORIGIN      
        1 cgtagacgag accgatcaac accgcagtgt tggtgaacaa gaaccgaatc ggctcccccc
       61 cgagggcgac gctgagctgg gagatcgggc cctccgagcc caggatcaca cgccaggcgt
      121 aggtcctgac caggaagttc gaccaaaacg ggatcatcac cagcaccagc aggaggttgc
      181 gggagcctcc ggaccgggtg gcgatgtaat aggcgaaggg gtaggacaag aggagacaca
      241 acgcggtcgt caccgaagcg agcacgaagg acctgacgaa gatctggagc acatccgcgg
      301 agaagagtcg cttgtacgag tcgaggttcc agtcctctag gacggtgaga cccgtcgagt
      361 ccctggtggc aaacgagtag acgaacacga ggagaagcgg gaagacgaag aggaggacga
      421 gatagatgaa ggcgggaagc gagatgaaga agccacgtct cgcctcggcc ttgatcgcag
      481 ctcgttccag ttccggggtg atgggggtcg tcgactcctt ctcgtcggcg atcggtgggg
      541 cggtcatcag ctgaggacca ggttggccgc ctcggggctc cacaccaggt aggcctcctc
      601 acccacgtcg tacaggtcga cgccggggcg gttctcctcc ttgacctcta tctcggtccc
      661 gattccgccg tcgacgtcgt agaaatagac atcgccgtag tagaggcgtc gcatcacccg
      721 caccttcacc cggttgtggc cgggcgggat ctcctcgtcg aggtcgtaga gagccagacg
      781 ctcggggcgg agagccaggg tgacctcggt cccggacggt gtggaagcgg caggcacccc
      841 gatctcgagc ccattgctga gccgggcgga gttggacccg tccatggtgg cgccgatcag
      901 attgatgtca ccgatgaagt cggcgacgaa tctggaggtg ggacgctcgt aaatctcctc
      961 ggacttcccc acctggagca ggcttccctc actcatgacg ccgatccgat ccgacatcgt
     1021 gagtgcctct tgctggtcgt gggtgacgta gatgaacgtg atcccgaccc gtccctggag
     1081 gtccttgagc tcgacctgca tcgcctgtcg cagcttcagg tcgagcgccc ccagcggctc
     1141 gtccaaaagc aggacctcgg gctcgttgac caatgcccgg gcgagtgcca cccgctgctg
     1201 catgccaccc gagagctgac tgggcttggc gtcggcccgc ttcgagagct gaacctggtc
     1261 caggactcgc gtcacccggc tcgcgacctc ggacttctcc accttcctca tgttcaggcc
     1321 gaaggcgacg ttctcggcca ccgtcatgtg ggggaaaagc gcgtatgact ggaacaccgt
     1381 gttgatcggc cgctggttcg gcggtttcag gcccatctcc gccccgtgga ccctgatgct
     1441 cccctcggtg gggaactcga agccggcaat catgcgaagg gtggtggtct tgccacaccc
     1501 ggaggggccg agcagggaga agaactcccc gtcgttgacc gtgaggtcaa ttcggttgac
     1561 cgccaccacc tcgtcaccga accgcttggt gacgtctgcc aactcgaccg ccggtttgga
     1621 catcaatcct cctcgaaagc cacctctccg tcgaccaccg tcatcgccac ggaagcctcc
     1681 cggattcgcc cgcccgccat cggatcccgg tccaggatca cgaggtctgc gagcttaccc
     1741 acagccaacg tcccgctgac gaggtcacga tgattgacat aggccgaacc ggcggtgaac
     1801 ccggccagcc cgtccagaaa cgaaatccgc tccccggtgt tgagtggcgg gagatcggga
     1861 gcttcgtggt tgagacgggt gaccgccgtg tcgatctggt ccatgacgtc cgcggtggag
     1921 accccccagt ccgatcccat cgccagggtc gcccccgccc tgagcaagct gccaaacgga
     1981 tactgccaaa ccgagcgctg ctcccccagg tatggcttgg tcaggtcgat ctggtcgatc
     2041 ccgtcctgag cccagagcgc ctgggcgttg gccacgacac cgagccgccg gaaccggggg
     2101 atgtcgtcgg ggtggacgac ctggatgtgg ctgatgtggt ggcgggcgcc ggtccacccg
     2161 ttgccgagcc gcgccgcctc gaccgcgtcc aacccgttgc ggaccgcggc gtcgccgatg
     2221 gcatggaagt ggcaggagaa cccgcgtcgg tccagttcgg tgacgatctc ctttagctcg
     2281 gcggggtcga tgaagtcgat gccgcggttc tcggtctctt gcccgtggcc atcgaggtag
     2341 ggctcgagaa gcgccgcggt gtggttctcg acgacgccgt cgagcatgag cttcaccgca
     2401 accgatcgat agcggccctc gggctcggac cgcatctgtt ccagagcgtc gatctgctcc
     2461 atccccagac cccggtccca ccacatggcg ccgaccgcgc tgcacaccag atcacccgac
     2521 ccggccagcg accgataggc ctgatgtgtg gcgagatcga catgtgcgtc ctgccaggcc
     2581 gtgattccct tggagaggag gtatcgctgt gccgccagga ccccgagacg catctcctca
     2641 ggtgtgtccc ggggcgccag tctctccacc agatgcgccg ccccctcgtg aagggtgccc
     2701 tggggccggc cgtcgggcgt ccgctcgatc ctgccatcgg ccgggtccgg gg
//
LOCUS       DATITW010000439         1555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_421950, whole genome shotgun sequence.
ACCESSION   DATITW010000439 DATITW010000000
VERSION     DATITW010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421950"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VLS86_06145"
     CDS             <1..1140
                     /locus_tag="VLS86_06145"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HSO50109.1"
                     /translation="GDIDIIVVTDDPGSVAAFVVKMPEAAEVLGSGETKTSFLTREGM
                     QVDVRSVSRDQLGSALLYFTGSKAHNISLRQRAIERGWLLSEYGLFEEDRLVASVTEE
                     DIYQALEMQYVPPPLREGTGEVEAAAASALPGLISRDQIRGDLHYHCTRSGDGRSSLE
                     EMVEAAVANGYIYVAFTDHGEDLAINGSTREEMLAHRDRIRALEAEHSGIRLLFGCEL
                     NIGPKGELDYDPEFRLEFDYCVASIHSHFDLSQSAQTARILTALADPAINAIGHLSGR
                     YIGRRPGVELDIDVVLEALAVTGVALEINGALDRLDASAEVARLAMARDVDFVIDTDS
                     HHVSDLVRMDYGVEYARRGWVTTDRVVNTRPVEDFMTWVGRRRGG"
     gene            1288..>1555
                     /locus_tag="VLS86_06150"
     CDS             1288..>1555
                     /locus_tag="VLS86_06150"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HSO50110.1"
                     /translation="MADTFVLDTCVLLADPQSPLRFDEHDVVLPLVVIEELDRKKTRL
                     DEVGANARAAIRLIEELGASRSGGLREPVALPGGGTLRIELNGIT"
BASE COUNT          274 a          482 c          515 g          284 t
ORIGIN      
        1 ggggacatcg acatcatcgt cgtgaccgac gacccggggt cggtggctgc cttcgtggtg
       61 aagatgcccg aggcggcgga ggtgctcggg tcgggggaga ccaagacgtc cttcctgact
      121 cgcgaaggca tgcaggtcga cgtcaggtcg gtgagtcggg atcaactggg gtcggctctg
      181 ctctacttca caggttccaa ggcccacaac atcagccttc gtcagcgggc catcgagcgg
      241 ggctggctgc tcagtgagta tggcctgttc gaggaggacc gcctggttgc gtcggtcacc
      301 gaggaagaca tctaccaggc actcgagatg caatacgtgc cgccaccact ccgggaagga
      361 accggtgagg tcgaagcggc ggcggcttcc gcgcttcctg gcctcatctc ccgggatcaa
      421 atcaggggcg acctccacta tcactgcacc cgatccggag acggtaggtc gagcctcgag
      481 gagatggtcg aggccgcggt tgccaacggc tacatctacg ttgcgttcac cgaccatggc
      541 gaagacctgg cgatcaacgg ctcgacacgc gaggagatgc tggcgcatcg tgatcggatc
      601 cgggctctcg aggccgagca ctcggggatt cgccttcttt tcggctgcga gctcaatatc
      661 ggtcccaagg gtgagctcga ctacgacccg gagttccggc tggagttcga ctactgcgtc
      721 gcctcgatcc actcccactt cgacctatcc cagtccgccc agaccgctcg catcctcacc
      781 gccctcgccg atccggccat caatgccatc gggcaccttt cggggcgcta catcggcagg
      841 cggcccggcg tcgagctcga catcgatgtg gttctcgagg cactggccgt gaccggcgtt
      901 gcccttgaga tcaacggtgc actggaccgc ctcgatgcgt ccgcagaagt ggcgcggctg
      961 gccatggctc gggacgtgga tttcgtgatc gacacggact ctcatcatgt atccgacctg
     1021 gtgcggatgg actatggagt ggaatacgcg cggaggggat gggtcaccac cgatcgggtc
     1081 gtcaacaccc gacccgtaga ggatttcatg acgtgggtag ggcggcgtcg aggcggatga
     1141 ctctgatccc ccgtaattgg gttgcgcagg ggtcacgtac ggcccggtag atcccaatga
     1201 cggctggggc gtctgcggtc cttgtatttg ccgtccgggg acgggaagct atggcccctg
     1261 gcgacgaccc tgaaggagac ccgcgaggtg gccgacacct ttgtcctcga tacctgtgtc
     1321 ctgctcgccg acccccagtc cccgctccga ttcgatgaac acgatgtggt gctgccgctc
     1381 gtggtcatcg aggagttgga tcggaagaag acccgactgg acgaggtcgg tgccaacgcg
     1441 cgagccgcga tcaggctcat cgaagagctc ggggcctctc gaagcggcgg gctacgagaa
     1501 ccagtcgccc tgcctggcgg cgggaccctg cgcatcgagc tcaacgggat cacat
//
LOCUS       DATITW010000440         1663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_341483, whole genome shotgun sequence.
ACCESSION   DATITW010000440 DATITW010000000
VERSION     DATITW010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341483"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..1578
                     /locus_tag="VLS86_06155"
     CDS             283..1578
                     /locus_tag="VLS86_06155"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSO50111.1"
                     /translation="MSNRLRRWGRLAVVDVTPLRQVPAYRWLFAGMFFAQAGRQLTVV
                     AVPWQVFELTGSTLAVGFLGLAQLIPLLMVSLIGGALADAVNRRRLLLLSQLTMALTA
                     GGLLWNSLADAPAVWPLFVLSAINAGVSAIDSPARHAIVPALVGRALLPSAMALNQTL
                     GNIASAAVPAVAGLLIATVGLPFNYGIEVIVFLAGAALIYKVPDVPTEGGGRKFGLSS
                     IMEGLRYLKGRKVIQSILLIDFNAMVFGMPTALFPAIGTQVFGGDAFTVGLLYAAPGV
                     GALAAALTSGWVGGVRRQGRAIVIAVIGWGLAIAIFGLTTSLALALIMLSIAGAGDVV
                     SAIFRGTIIQLSAPDALRGRMSSMHMAVVASGPRLGDLEAGVVAAVTSIRVSVVSGGL
                     ACVLGAIAIARWAPEFANYLYEPEGKYPESPERTGELSP"
BASE COUNT          233 a          539 c          568 g          323 t
ORIGIN      
        1 gagaattcgc tggtgaacct gggtgcggtc tcctcgtggg tgacatgctg gaaggtcacc
       61 gtcttcagat ggccggggta caccttcatc ccctcgtaga tcggctgcga gagatcgacc
      121 agtctcatgt gtctccttga tagcgggcgt tccgagtcgg cgagatgatc ccatacaata
      181 cggcgctgac aggtcagctt cccaccgggt cggatcaccg aggaccgact cgggtgatcc
      241 ccggataggt cacgcgggcg aggccaaggg aagggtgatc agttgagcaa tcgtctgcgg
      301 cgttggggcc gtctcgcagt ggtcgacgta acgcccctgc gtcaggtgcc cgcctatcgg
      361 tggctgttcg ccggcatgtt cttcgcgcag gccgggcgcc agctgacggt cgtggccgtg
      421 ccctggcagg tcttcgagct caccgggtcg accctggccg tcggcttcct cggcctggcc
      481 cagctgatcc cgctactcat ggtgtcgctg atcggcggag cgctggcgga cgcggtgaac
      541 cggcgacggc tcttgctcct ctcgcagctg acaatggcgc tgactgcggg agggctgttg
      601 tggaactccc tggccgatgc tcctgcggtc tggcctctgt tcgtcctgag cgcgatcaat
      661 gccggtgtct ccgccatcga ctcgccggcc cgtcacgcca tcgtccccgc cttggtgggt
      721 cgggcgctcc tcccctcggc gatggcgctg aaccagacac tgggcaacat cgcctcggcg
      781 gcagtgcccg cggtggctgg tctgctcata gccacggtgg ggctcccgtt caactatggc
      841 atcgaggtca tcgtgttcct ggccggcgcc gcactcatct acaaggtgcc ggacgtgccc
      901 acggagggtg gggggcggaa gttcggcctc tcctcgatca tggagggtct gcgctacctg
      961 aaggggagaa aggtgatcca gagcatcctc ctcatcgact tcaacgccat ggtgttcggg
     1021 atgccgacag ccctcttccc tgcgatcggg acacaggtct tcggagggga cgcgttcacc
     1081 gtcggcttgc tctatgcagc tcctggggtc ggcgccctcg ccgccgccct gacctcggga
     1141 tgggtcggtg gtgtgcgccg ccagggacgg gccatcgtga tcgcggtcat tggctggggg
     1201 ctggcgatag cgatattcgg gctgaccacc tctctcgccc tcgccctgat catgctctcc
     1261 atcgccggcg ccggcgacgt ggtgtccgcc atatttcgcg gcaccatcat ccagctgtcg
     1321 gctcccgatg ccttgcgcgg ccgaatgtcg tcgatgcaca tggcggtggt ggccagcggc
     1381 ccgagactgg gagatctcga ggccggggtg gtcgccgcgg tgacctcgat cagggtctcg
     1441 gtggtatcag gtggtctcgc ctgtgtgctg ggcgccatcg ccatcgcccg ctgggccccg
     1501 gagttcgcca actacctgta cgagcccgaa ggcaagtacc cggaaagccc ggagcggacg
     1561 ggggaactga gcccatgagc cgacccctca cctgcgcgat gcgggcagcc gaggtctcgg
     1621 tagaggtctc agccgagccc gaagactcgg gtggcgttgc ccc
//
LOCUS       DATITW010000441         1914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_61779, whole genome shotgun sequence.
ACCESSION   DATITW010000441 DATITW010000000
VERSION     DATITW010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61779"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..699)
                     /locus_tag="VLS86_06160"
     CDS             complement(7..699)
                     /locus_tag="VLS86_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HSO50112.1"
                     /translation="MPTAFAILSLLAALVPGASPEYVPAAVSPEPISEYVPAGDEAYH
                     LDLEVGGYVNGAMDTARLMSYRGCTLERDAAYMYALLLEAAEADGIRLSPVDCYRSYG
                     NQKAAYNRRCPESEVDVYAVDPVTGEKAPAGTKTVRVCTGPPTARPGYSNHGWGRAVD
                     FGDGSGVLGCSDRALRWLQGNAYRFGWVHPRWAWCDMSTREPWHWEWAGVTDPTLVGY
                     YEFNEELIQIVR"
     gene            complement(759..>1914)
                     /locus_tag="VLS86_06165"
     CDS             complement(759..>1914)
                     /locus_tag="VLS86_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSO50113.1"
                     /translation="EAGKLRDKEKELLAAKEGKEREIKDSGVDLFDEVDEEAIAEVLS
                     MWTGIPVYKLTEEETQKLLHMEDDLHKRVIGQEDSIRAVAQAVRRTRAGLKDPKRPSG
                     SFIFLGPSGVGKTETAKALAEFLFGKEDALIQLDMSEYMEKHTVSRLVGSPPGYVGYD
                     EGGQLTEAVRRKPFSVVLFDEIEKAHPDVFNTLLQILEEGRLTDAQGRTVDFKNTVII
                     MTSNLGTRDLHKKAVGFHVEDEEISYEYMKGRVTEELKKHFRPEFINRLDEIIVFHEL
                     TMEELKQIVDLMLLRVTDQLTSQHLDLVLTDDAKEYLGSRGYDPELGARPLRRAIQRL
                     LEDPLSERVLLGEFKAGTTIMVSLDSVNDELAFEAVTTPNTPTVEMAGQD"
BASE COUNT          320 a          611 c          610 g          373 t
ORIGIN      
        1 ggctgatcag cggacgatct ggatcagttc ctcgttgaac tcgtagtagc cgaccaaagt
       61 gggatcggtg acgcctgccc actcccaatg ccacggctca cgggtgctca tgtcacacca
      121 ggcccacctc gggtggaccc atccgaatcg gtaggcattt ccttgaagcc aacgcaatgc
      181 gcgatcgctg cagccgagca cgccggagcc atcgccgaag tcaacggccc gaccccatcc
      241 atggttcgag tagcccgggc gggcggtcgg ggggccggtg cacacccgaa ccgtcttcgt
      301 cccggccggt gccttctccc cggtaaccgg gtcgacggca tagacatcga cctccgactc
      361 cgggcatcga cggttgtagg cggctttctg attcccgtac gagcgataac agtcgaccgg
      421 gctcagccga atcccgtctg cctcggccgc ctcgagcagc agggcgtaca tgtaggcggc
      481 atcgcgttcc agagtgcagc cgcgatagct catgagacgg gcagtgtcca tggctccgtt
      541 cacatagcca ccgacttcta ggtcgaggtg gtacgcctcg tcaccggccg ggacgtactc
      601 ggatatcggc tccgggctga ccgcagccgg gacgtactca ggagaggcac ctgggaccag
      661 cgcggcaagg agcgacagta ttgcgaaggc tgtgggcatg actatggcgg gcttctagcc
      721 cccataggat cggccagtgg acagggcccc tttagcggtc agtcctgacc ggccatctcc
      781 actgtcggcg tgttgggcgt ggtcaccgct tcgaatgcga gttcgtcatt cacgctgtcg
      841 agggacacca tgatcgtggt ccctgccttg aactcgccaa gcagcacccg ctctgacaac
      901 ggatcctcga gcagcctctg gatcgcgcga cgcaacggcc gggccccaag ctcggggtcg
      961 tagccccttg agccgaggta ctccttggcg tcgtcggtga gcaccaggtc gagatgctga
     1021 ctcgtgagct gatcggtgac gcgaagcagc atgaggtcga cgatctgctt cagctcctcc
     1081 atcgtcagct cgtggaagac gatgatctca tcgaggcgat tgatgaactc gggccggaag
     1141 tgcttcttga gctcttcggt gacccggccc ttcatgtact cgtaggagat ctcctcgtcc
     1201 tcgacgtgga agccgaccgc cttcttgtgc aggtcccgag tccccaggtt ggaggtcatg
     1261 atgatcactg tgttcttgaa gtcgacggtg cgaccctggg catcggtcag cctgccctcc
     1321 tccaagatct gaagcagggt gttgaagacg tcggggtgtg ccttctcgat ctcgtcgaag
     1381 agcacgaccg agaatggctt gcggcggacc gcctcggtga gctggccacc ctcgtcatag
     1441 ccgacatacc cgggtggcga gccaaccagg cggctgacgg tgtgcttctc catgtactcg
     1501 gacatgtcga gctggatgag ggcatcttcc ttgccgaaaa ggaactcggc caaggccttg
     1561 gcggtctcgg tcttcccgac gccggacggg ccgaggaaga tgaagctgcc gctggggcgc
     1621 ttcgggtcct tgaggccggc ccgggtgcgg cggacggcct gggccacggc ccggatggag
     1681 tcctcctggc cgatcacgcg cttgtggagg tcgtcctcca tgtggaggag cttctgcgtc
     1741 tcctcctcgg tgagcttgta cacggggatg ccggtccaca tcgacaggac ctcggcgatg
     1801 gcttcctcgt cgacctcgtc gaacaggtcg acgccggaat ccttgatctc gcgctccttg
     1861 ccctccttgg cggcgagcag ctccttctcc ttgtcgcgga gcttgccggc ctcc
//
LOCUS       DATITW010000442         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_341704, whole genome shotgun sequence.
ACCESSION   DATITW010000442 DATITW010000000
VERSION     DATITW010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341704"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VLS86_06170"
     CDS             <1..432
                     /locus_tag="VLS86_06170"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HSO50114.1"
                     /translation="RATTLAATDDQLTLTGAGVELVFNKLPPVPTAALTNTVWVLETL
                     IEGDSASSVGGERATLELFTDGSMLGSTGCRNLHGRYVVSGAEIQMPEFAAEGECPQE
                     LEAQDGMVVTVLGDGFRVLVEGEVLTLTSAGNLGLIYHAEG"
     gene            complement(533..970)
                     /locus_tag="VLS86_06175"
     CDS             complement(533..970)
                     /locus_tag="VLS86_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSO50115.1"
                     /translation="MNITAHAWMRAAPFLRSFVPSGEVATDAYPDPPFQIEGDLRLGE
                     LWGVKYFDGSSFGRSMSMSTKLYVGNLSFTTDSAELESLFAPHGRVTKAQVINDRDTG
                     RSRGFGFVEMDSAEEAQAAISSLNGKDMGGRQLTVNLAKERSR"
     gene            1242..1481
                     /locus_tag="VLS86_06180"
     CDS             1242..1481
                     /locus_tag="VLS86_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50116.1"
                     /translation="MSKRARERTRQEKQEAKRQRKEDRVEESAQAVLPDETALMEEFR
                     ALSEQYNANLVSESHYAEERHRIFVEWGIETEQDA"
     gene            complement(1490..1873)
                     /locus_tag="VLS86_06185"
     CDS             complement(1490..1873)
                     /locus_tag="VLS86_06185"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50117.1"
                     /translation="MVFIWFGALKVFGVSPVGDLVASTVYWVDPDWFVPALGVIEVLV
                     GVGLAARFGLRLVLLVLALQMLGTFLVFVLLPELTFQDGNPLQLTIEGEFVLKNLVLL
                     ASAMVVGASIDSHEVLVVDQEPATR"
BASE COUNT          454 a          643 c          647 g          355 t
ORIGIN      
        1 cgggcaacga cgttggccgc caccgacgat cagctgaccc tcaccggcgc tggtgtcgaa
       61 ttggtcttca acaagttgcc cccggtaccg accgcggctc tgaccaatac cgtctgggtg
      121 ctggagactc tgatcgaagg cgactcggct tccagtgtcg gcggtgagag agcgaccctg
      181 gaactgttca ccgacgggtc gatgctcgga tcgacaggtt gtcgcaacct ccacggcaga
      241 tacgtggtga gtggagccga gatccagatg cccgagttcg cggccgaggg cgagtgtccg
      301 caagagctcg aggcgcagga cgggatggtg gtgaccgtgc tcggcgacgg gttccgggtg
      361 ctggtcgagg gggaagtcct caccctgacc tctgcgggga acctgggtct gatctatcac
      421 gccgagggct gataccccca cgatccagaa cgtgagaaac cccccggaga accggggggt
      481 ttctctgtgt tgatcgaggc tcgacggcga aagccgtctc gccaacgact gcttagcggg
      541 aacgctcttt ggccaggttc acggtgagct gacgcccgcc catgtccttg ccgttcaacg
      601 aggagatcgc agcttgcgct tcctcggccg aatccatttc gacgaacccg aaaccacgag
      661 acctaccggt gtcgcggtca ttgatgactt gcgccttggt cacacgaccg tggggcgcga
      721 atagggactc cagctctgcg gagtcggtgg tgaacgaaag gttccccaca tagagcttgg
      781 tggacattga catgctcctt ccgaagctcg aaccgtcgaa gtacttgacg ccccacagct
      841 cgcccaaccg aagatcacct tcgatttgaa acggagggtc tgggtaggca tctgtggcga
      901 cttcacccga cggtacgaag gagcgaagaa acggcgcagc acgcatccag gcgtgcgctg
      961 tgatgttcat agtagctgct tcggcccgat attccgagtc cgccgccgcc gatggtggtc
     1021 gacgaccgtg cagaggtttc ctgtctaggg gcgcttcggg gttagggtgg taggtgcgta
     1081 tctcaccacc aggcatcgcc gggaccaccc gcgaccgacc aggtaggacc ggatggattg
     1141 attcggtctc gggctctgtg aatgtcaatg ttcgggaaac ccggtgagtt gtcgccttcc
     1201 gcgaccagag gatccacatg gcacaacgag gacgtgaggc aatgagcaaa cgggcccgcg
     1261 agcggacccg tcaggagaag caagaggcca aacgccagcg caaggaagac cgcgtcgagg
     1321 agtcggccca ggctgtgttg cccgacgaga ccgccctcat ggaggaattc agggctctca
     1381 gcgagcagta caacgccaac ctggtgagcg aaagccacta cgccgaggag cggcatcgca
     1441 tcttcgtcga gtgggggatc gagaccgagc aggacgcgta gccgtcggcc tacctcgtcg
     1501 ccggctcctg atccaccacc aacacctcat gtgaatcgat gctggcgccc accaccattg
     1561 ccgacgccaa caggaccagg ttcttgagca cgaactctcc ctcgatcgtc agctgcagag
     1621 ggttcccatc ttgaaacgtg agctcgggca gcagcacaaa caccaggaag gtgcccagca
     1681 tctgtagggc gagcaccaac agcaccagcc gcaaaccgaa tcgagcggcg agaccgaccc
     1741 cgaccagcac ttcgatcaca cccaaggcag ggacgaacca gtccggatcg acccagtaga
     1801 ccgtcgatgc gacgaggtcg cccaccggtg acaccccgaa gaccttcagc gcgccgaacc
     1861 agatgaacac cactgccagt ggatatccgc aacgtgggta tcccccagcg gcggagaaac
     1921 gggataacga cctcatccac ccgcgcgatg aacgtgttca cacctcgatg atcctggatc
     1981 tggcggaatg tgagaaattg aagaccaaga ggtgacgaaa gacccctctt tgtttctcgg
     2041 cggaccctat tgcccgacgg tcgagaatcg ttaacttcgt actgtccgcc acaaggaac
//
LOCUS       DATITW010000443         1675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_782377, whole genome shotgun sequence.
ACCESSION   DATITW010000443 DATITW010000000
VERSION     DATITW010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782377"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VLS86_06190"
     CDS             <1..359
                     /locus_tag="VLS86_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HSO50118.1"
                     /translation="NVVNMALIGAVGGYLIYRSLMRMTRSKPSGVPLLAAIAAMLSVP
                     LAAMGFVVEFWLGGTLDSISISSVLWAMLGTHLVIGVGEGVITFFVVAAVMRARPDLV
                     YGSQSFTGARSEELVP"
     gene            356..652
                     /locus_tag="VLS86_06195"
     CDS             356..652
                     /locus_tag="VLS86_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HSO50119.1"
                     /translation="MKRGLAIFAFGLLVTVVVAMVLSQFASGQPDGLEDVAAEQGFLD
                     AAEEHALADTPLADYGGDSRDRLMLAGLVGVLATLGLGYLVFSLVKPRETTGEQ"
     gene            649..1413
                     /gene="cbiQ"
                     /locus_tag="VLS86_06200"
     CDS             649..1413
                     /gene="cbiQ"
                     /locus_tag="VLS86_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HSO50120.1"
                     /translation="MSGAHGHALYVHARSPLHRLAPQVKIVSALLLLFAVISTPRQAF
                     WAFAIYAAVLAALAALSRIPIRFLLSRMLVAAPFVLLALLFPIVGSGPRVEVLGLSLS
                     QAGLWDMWNLLAKSLLGLLTAIILGATTEVADLLRGLSALRVPGLVTSILGFMVRYLD
                     VIIGDLTRMRLALAARGHHPTSIGDWAPYGKAVGAMFIRTYERGERVYLAMESRGYSG
                     RMPGSAAIEAKGLEWAVGLVPAILAWAVALTARTAA"
     gene            1410..>1675
                     /locus_tag="VLS86_06205"
     CDS             1410..>1675
                     /locus_tag="VLS86_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO50121.1"
                     /translation="MSVPVIEIMGLSFSYPDGTIALTDIDLHIHRGERVALLGPNGAG
                     KTTLILHMNGIHRPQTGTVTVSGMAVTAPNLLEIRRRVGLVFQDP"
BASE COUNT          238 a          552 c          543 g          342 t
ORIGIN      
        1 tgaacgtggt caacatggca ctgatcggtg cggtcggcgg ttacttgatc tatcgctcgt
       61 tgatgaggat gacccggtcc aagccgtcgg gcgtccccct tctggcggcc atcgcagcca
      121 tgctctccgt ccccctggcg gcgatgggtt tcgtggtcga gttctggctg ggtgggactt
      181 tggacagcat ctcgatttct tcagtgctgt gggccatgct cggcacccat ctcgtgatcg
      241 gcgtcggtga gggcgtgatc acctttttcg tggtcgccgc tgtgatgaga gcccggccgg
      301 atctggtcta cggatcgcag agcttcactg gagcccggag cgaagagctg gtgccgtgaa
      361 gcgaggtctg gcgattttcg cattcgggct cctggtgaca gtggtcgtgg ccatggtgct
      421 ctcccagttc gcatcgggcc aacccgacgg gctcgaagac gtggccgcgg agcagggctt
      481 ccttgacgca gccgaggaac acgctctcgc cgacaccccc ctcgccgact atggagggga
      541 cagccgagac aggctgatgt tggccggtct cgtcggtgtc ctggccacac tcggcctggg
      601 gtatctggtg ttctcactgg tcaagcccag ggagaccacg ggcgagcaat gagcggggcg
      661 cacgggcacg ccctatacgt acacgcccgg tcccccctgc atcgtctcgc accccaggtc
      721 aagatcgtca gcgcactcct gttgctcttc gccgtgatca gcaccccccg ccaggcgttc
      781 tgggcgttcg ccatctatgc cgcggtcctc gccgccctgg ccgctctgtc ccggatcccg
      841 attcgcttcc tgctctcccg catgttggtg gcagccccgt tcgtgctcct cgccctcctg
      901 ttcccgatcg tcgggtcggg accgcgtgtc gaggtccttg ggctttcctt gagccaggcc
      961 gggctctggg acatgtggaa cctcctggcc aagtctctgc tcggcttgtt gactgcgatc
     1021 attctcggcg ccaccaccga agtggccgat ctcttacgag gcctgagcgc gttgcgggtt
     1081 cccgggctgg tgacatcgat ccttgggttc atggtccgct acctcgacgt gatcatcggc
     1141 gacctgaccc gaatgcggct cgcccttgct gcccggggcc accatcccac ctcgatcggg
     1201 gactgggcgc catacggcaa ggcggtaggg gcgatgttca tcaggacgta cgagcgaggg
     1261 gagcgggtct acctggccat ggagtcgcgc ggctattcgg gccggatgcc cggttcggcg
     1321 gccatcgaag cgaaggggct ggagtgggcg gtcggcctgg tgcccgccat cctcgcctgg
     1381 gcagtagcgc tgaccgcgag gaccgcggca tgagcgttcc ggtcatcgag atcatgggac
     1441 tctcgttctc ctatcccgac ggcacgatcg ccctcaccga catcgacctt cacatccacc
     1501 gtggggaacg ggtcgccctc ctcgggccga atggagcggg caagaccacg ttgatcctcc
     1561 acatgaacgg gattcatcga cctcagaccg gaactgtgac ggtgtccggc atggcggtca
     1621 ccgcgcccaa cctcctcgag atccgtcgcc gggtcggcct ggtcttccag gatcc
//
LOCUS       DATITW010000444         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_422218, whole genome shotgun sequence.
ACCESSION   DATITW010000444 DATITW010000000
VERSION     DATITW010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422218"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..1261
                     /gene="cpaB"
                     /locus_tag="VLS86_06210"
     CDS             338..1261
                     /gene="cpaB"
                     /locus_tag="VLS86_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HSO50122.1"
                     /translation="MGRRAIVLLVALILAGLAAWAVWNYLQGIENEIEAGQEAVTVYR
                     AVDAIAEGTDGAIILSDFNSGGQLLEAGTEEREDLPDGAITTEEQLNQVLGNTVAAGP
                     ISANSILVESQWTAITIDVTPLAEQIPEGKQAIVVSTDNVRGVNGFVNPGDRVNMIIT
                     LDIPEDLLPEELQGIATPAAPPAEGEAPTEEDIAVVTFTRYVLQGIPVLATGRDIRAD
                     EDAPPQVEAEPEAPVEGETDTTGTEEEDTGNETIFTLEVTPEQAERIAYAFENGSMWL
                     TLVPEDFVEVETPGVTVLNLFGGNLAEDIFG"
     gene            1289..>2258
                     /locus_tag="VLS86_06215"
     CDS             1289..>2258
                     /locus_tag="VLS86_06215"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSO50123.1"
                     /translation="MAEHNLLVVDKDDDFVSAAKELFEGRLPVARSIEEASQTIESGE
                     LRMLLLGPSYSNEESMEQVRSLHNQDPVLVLMMVAETVTADLLRYAMRAGVSDVIESP
                     LDETKIEAAIEQFAHDVLKRRNVASRTSRESDRSERGQLITVTSAKGGSGKTVMATNV
                     ALLLNRLPDKKVVLVDADLQFGDVCLLLQLEPRFTMVNAAHELHQLDSEMLDSLLTEH
                     PSGLRVLAAPLEPAFADDITTAGLMQMLDVLQETYDYIVVDTASMLDELILSLIEKAD
                     QVLMIVDMDLPSVKNAKLALETLRLLKFSTANVKLVMNRSNSKARLD"
BASE COUNT          467 a          679 c          702 g          410 t
ORIGIN      
        1 gaggccatcc tccaagagct caaggagcgg ggcataggcc gcaggtgacc gttggtcgct
       61 tgttcgactg tcttccggaa tggcgacagg tactagtcat ttcgtcacgc aatcgaggtg
      121 tgagctagtc acgcctcgaa acccgggtca cgatatgaga cgaccggact atggaggttc
      181 tggggaaagt ctcaagactt gctccataag gtccgaatca ttcatgtgga attggacttg
      241 aaacactggg agggcgcgcc ccaaccggta gcatgccaaa cggtatgtgc cggcgacggg
      301 tcggcgccca accgagggga caaacgaagg ggactcaatg ggtaggagag ccatcgttct
      361 gcttgtggcc ttgatactgg ccgggctcgc cgcttgggcg gtctggaact acttgcaggg
      421 catagagaac gaaatcgaag caggccagga ggcggtcacc gtctaccgcg ccgtcgatgc
      481 catcgctgaa gggaccgacg gggccatcat cctctcggac ttcaactcag gtggccagct
      541 tctcgaggcc ggcaccgaag agcgggaaga cctgcccgac ggagccatca ctaccgaaga
      601 gcagctcaac caagttttag gcaacacggt cgcagccggc ccgatttcgg cgaactcgat
      661 tctggtcgag tcgcagtgga cggcgatcac catcgacgtc acccccctcg ccgagcaaat
      721 ccccgagggc aagcaggcca ttgtggtctc cacggacaac gtgcgtggtg tcaacggttt
      781 cgtcaatccg ggtgaccgtg tcaacatgat catcactctc gacatccccg aggacctgct
      841 tcctgaggaa ctgcagggga tcgcgacccc cgcggctccg ccggcggaag gcgaagcacc
      901 gaccgaggag gatatcgccg tcgtcacctt cacccggtac gtcctgcagg ggatcccggt
      961 gctggcgacc ggtcgtgaca tccgcgccga tgaggacgcc ccgccgcagg tggaagctga
     1021 gccggaggcc ccggtcgaag gcgagaccga caccacaggt accgaggaag aggacaccgg
     1081 caacgagacc atcttcaccc tggaagtcac tcccgagcag gccgagcgca tcgcatacgc
     1141 cttcgagaat ggttccatgt ggttgaccct cgttcccgaa gacttcgtcg aggtggagac
     1201 cccgggtgtg accgtcctca acctgttcgg tgggaacctg gcggaagaca ttttcggata
     1261 gttttgagta agaggttcga ccatgtcatt ggctgagcac aacctattgg tggtcgacaa
     1321 ggacgatgac ttcgtctctg ccgccaagga gctcttcgaa ggccggctcc cggtagcccg
     1381 ctccatcgaa gaggcgagtc agaccatcga gtctggcgag ctgcggatgc tcctgctcgg
     1441 gccctcgtac agcaacgagg agtccatgga acaggtgcgg agcctgcaca accaggatcc
     1501 ggtgctggtc ctgatgatgg tggccgagac ggtcaccgcc gatctccttc ggtacgcgat
     1561 gcgtgccggt gtctcggacg tgatcgagtc cccactcgac gagacgaaga tcgaggcggc
     1621 gatagagcag ttcgcccacg acgtcctgaa gcggcgcaac gtcgcatccc ggacatcgcg
     1681 tgaatcggat cggtccgagc gaggccagct gatcaccgtc acctctgcca agggcggaag
     1741 cggcaagacg gtgatggcga ccaatgtcgc tctgttgctg aaccggctgc cggacaagaa
     1801 agtggtgttg gtcgacgccg acctccagtt cggcgatgtc tgcctgcttc tccagctcga
     1861 gccgcgcttc accatggtga acgcagctca cgagcttcat cagctggatt ctgagatgct
     1921 cgactcgttg ctcaccgagc acccgagcgg gcttcgggtg ctggcggcgc cgttggagcc
     1981 ggcgttcgcc gacgacatca cgaccgccgg cttgatgcag atgttggatg tcctccagga
     2041 gacctacgac tacatcgtgg tggacaccgc ctcgatgctc gacgaactga tcctttcact
     2101 catcgagaaa gcggatcagg tgctgatgat cgttgacatg gatctacctt cggtgaagaa
     2161 cgccaagttg gctctggaga ccttgcgtct cctcaagttc tccacggcca acgtgaaact
     2221 cgtcatgaac cgcagcaact ccaaggcgcg tctcgaca
//
LOCUS       DATITW010000445         1586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_102081, whole genome shotgun sequence.
ACCESSION   DATITW010000445 DATITW010000000
VERSION     DATITW010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102081"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..>1586
                     /locus_tag="VLS86_06220"
     CDS             157..>1586
                     /locus_tag="VLS86_06220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSO50124.1"
                     /translation="MHRIELAGLEGGSRVLSEEEVETFVAGLDGTVAFPGDEIYTEAT
                     SIWNGMIERRPAMVVRARTVNDVVRTVTMTRENGVELSIRGGGHNIAGLALTNGGLTL
                     DLSTMTTVQVDVAARRAKVEAGCTLGDVDRATQEHGLAATLGFVSATGVAGLTLGGGF
                     GYLTRQFGWTVDDLVEVELVTASGELLRASRSEHPELFWGLRGGGGNFGVVTEFVFDL
                     HQIGPQVTAGLIAWSAEVAEDALELYREVTAAAPRQLTIAALMRNAPLAPWLPEDKHG
                     KPMIAFVVTHTGTEAEASSDLAPVRSFGEPWADLIQVKDYAAQQAMLDATQPQGMHYY
                     WKSEFVPGLTDGFLSTYRTQFVDQVAPANQIVLFHLEGTLGERAEDDGAVGNRDAAYA
                     CVVQSMFPGGSPSAEENKQWVRSAWQALKPFSTGGNYINFQTDDESGERIAESYRDNL
                     DRLARIKAKYDPNNLFRVNRNIRPVS"
BASE COUNT          299 a          490 c          515 g          282 t
ORIGIN      
        1 ccatgggttg gtagggatga ccgagatagt gagaaatggc tacccgggaa accgggaagc
       61 aacactcagg actggtcaag tccaagccca tccctatctg tatcctcacg cctgcagggg
      121 acccctatgg ttcgcgttga ggcttggagg agatgcatgc accgaattga gctggccggg
      181 ttggaaggtg gctcccgggt cctgagcgag gaagaagtcg agacgttcgt agccggtctg
      241 gacggaacgg tggctttccc aggggacgag atatacaccg aggccaccag catctggaac
      301 gggatgatcg agagacgtcc ggcgatggtg gtccgggcga ggaccgtgaa tgatgtggtg
      361 cgcaccgtca ctatgactcg cgaaaacggt gttgaactat cgatccgagg tggcggccac
      421 aacattgccg gtctcgcact gaccaacggt ggtctcacct tggacctgtc gacgatgacc
      481 accgtgcagg tcgacgtggc ggcgaggcgg gcgaaggtgg aggccggttg caccctgggc
      541 gacgtggacc gggcgaccca ggaacacggc cttgccgcca ccttgggatt cgtctcggcc
      601 accggcgtgg cggggctcac cctgggggga gggttcggct atctcacccg ccaatttggc
      661 tggaccgtgg atgaccttgt cgaggttgaa ctggtcacag cgtccggtga actgctccga
      721 gcctctcgat ccgagcatcc ggagctcttc tgggggctgc ggggcggtgg cggcaacttc
      781 ggggtggtca ctgagttcgt gttcgacctt catcagattg gtccccaggt gaccgccgga
      841 ctgatcgcct ggtcggccga ggtggccgag gacgcactcg agctgtaccg agaagtgacc
      901 gccgccgccc ctcgtcagct caccatcgcc gccctcatgc gcaatgcccc gctggcgccg
      961 tggctgcccg aggacaagca cggcaaacct atgatcgcct tcgtggtcac ccacaccggg
     1021 accgaggccg aggcctcctc cgacctcgct ccggtccggt ccttcgggga accgtgggcg
     1081 gacctcatcc aggtgaagga ctacgcggct caacaggcga tgctcgatgc cacccagccg
     1141 caggggatgc actactactg gaaatctgag ttcgtccctg ggctgacgga cgggttcctc
     1201 tccacgtatc ggacccagtt cgtcgatcag gtggcccccg ctaaccaaat cgtcctcttc
     1261 cacttggagg gcactttggg cgagcgtgcc gaggatgacg gagcggtggg taaccgcgac
     1321 gctgcctacg cgtgcgtcgt ccagtcgatg tttcctggag gcagcccctc cgccgaggag
     1381 aacaagcagt gggtccgctc cgcctggcag gcactcaagc cattctccac cggaggcaac
     1441 tacatcaact tccagaccga cgacgagtcc ggagaacgca tcgccgagtc ctatcgagac
     1501 aatctcgatc gtctggcccg tatcaaggcg aagtacgacc cgaacaactt gttccgggtc
     1561 aaccgcaaca tcagaccggt ctccga
//
LOCUS       DATITW010000446         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_141632, whole genome shotgun sequence.
ACCESSION   DATITW010000446 DATITW010000000
VERSION     DATITW010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141632"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1108
                     /locus_tag="VLS86_06225"
     CDS             170..1108
                     /locus_tag="VLS86_06225"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HSO50125.1"
                     /translation="MYGITLAVAACLRSGTRADVAWMVAAQGLPIDDWSDALVFTRGG
                     GRIGSMAGGALDSKLADLAGRWSAGRLVDFEVSEIDALIAGLPSAGAARCVLVPADAL
                     PGDLWDLAAKREPVCLVCRLESDEVAEIQLYTTATIGEAGEGAEQLFATGSSGSVVSD
                     DVVITVFRPVPQLIVIGNTPVADALAELAALVGWRARILTDSTTATGVIASLSGLDKV
                     VVTGHDLDLTGAALMAALDSEVGYIGALGAHHMQETRADWLAYRGVTDLSRVHGPAGF
                     DIGAESPAEIAVSILAEAIAANLSDSGQAVERADAD"
     gene            1157..1408
                     /locus_tag="VLS86_06230"
     CDS             1157..1408
                     /locus_tag="VLS86_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50126.1"
                     /translation="MTGQDDTTADVLQSVWLTSHDHNELDEDCSPTKVCRNMKDVTFD
                     GYAEPGTDLGAAWRAKLAREGKLAPTGGSVRELILGQGE"
     gene            1452..2345
                     /locus_tag="VLS86_06235"
     CDS             1452..2345
                     /locus_tag="VLS86_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSO50127.1"
                     /translation="MSDDPTPTSSAGVAELTERLRRGGYLADRGLATSVYVAMALGRP
                     LLLEGEVGVGKTELAKVLATVLDRRLIRLQCYEGIDTHQALYEWDYARQMLQIRALSE
                     HNLADESQVDKLFGPKFLLERPLLTAVREGDRAVLLIDEIDRADDEFEAFLLELLSDY
                     QITIPEIGTIVAEAPPLVILTSNRTRELHDALKRRCMYHWVGYPTTEREVEIIMVRAP
                     EAGETLARKVVEAVHRLRGLDLAKPPGVAETLDWVATLNLLDRPDLDASTVRDTLGAV
                     VKERDDLELVLEHLEEIAVDG"
     gene            2338..>2672
                     /locus_tag="VLS86_06240"
     CDS             2338..>2672
                     /locus_tag="VLS86_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50128.1"
                     /translation="MVEQPSTLDLVVGDETPFIRMLVDFGERLRKAGLPVGSGDLLAY
                     CGAMAALNPGDLLDLYWAGRTTMVTRRDHIPVYDSVFRSFFLDSGGDVPEPLALVLEQ
                     SSIENQSVI"
BASE COUNT          447 a          871 c          906 g          448 t
ORIGIN      
        1 aggctctcgt cggcgaagta gtcgtggtcg tcgagggtct gatccagctc gtcgattgtg
       61 gtcggaatca catggacaga ctagggagaa gcggtgggtt gggcggaacg ggccgccgtg
      121 tggccggggc gatcaggtcc cggcgcggtc tggtccatac tattggcctg tgtacgggat
      181 cacgctcgcc gtggccgctt gcctgcgctc tggcacccgg gccgatgtcg cctggatggt
      241 ggcggcccaa gggctgccga tcgacgactg gtctgacgca ttggtgttca cccgcggcgg
      301 cggccggatc gggtccatgg ccgggggtgc cctcgacagc aagctggccg atctcgccgg
      361 acgatggagc gcgggccgcc tggtcgactt cgaggtcagc gagatcgacg ccctgatcgc
      421 cggattgccc tccgccggcg ccgcccggtg cgttctggtg ccggccgatg ccctcccggg
      481 agatctgtgg gacctggcgg cgaagcgcga accggtgtgc ctggtgtgcc gtctggagag
      541 cgacgaggtg gccgagatcc agctctacac gaccgccacc atcggcgagg cgggggaggg
      601 ggccgagcag ttgttcgcca ccggcagcag cgggtcggtg gtcagcgacg atgtggtgat
      661 caccgtgttc cggccggtgc cccagctgat cgtgatcggc aacaccccgg tcgcagacgc
      721 ccttgccgag ctggcggcgc tggtcggctg gcgggcccgc atcctcacgg actcgaccac
      781 ggcgaccggt gtcatcgcct ccctctccgg gttggacaag gtggtggtca ccgggcacga
      841 cctcgacctc accggtgctg ccctgatggc tgcccttgac agcgaagtgg gctacatcgg
      901 ggcgctcggc gcccaccaca tgcaagagac ccgggccgac tggctcgcct accggggggt
      961 gaccgatctg tcacgggtac atggcccggc agggttcgat atcggcgccg agtcaccagc
     1021 agagatcgcg gtctcgatcc tggccgaagc catcgccgcc aacctcagcg acagtggcca
     1081 ggctgtagag cgagccgatg ccgattagac tcaggcgctc accatccatt gcgttaccgc
     1141 tttccaggag caccaaatga ccggccaaga cgacaccaca gctgacgtcc tgcagtcggt
     1201 ctggctgacc agccacgacc acaacgagct ggatgaggac tgcagcccaa ccaaggtgtg
     1261 ccggaacatg aaggatgtga ccttcgacgg gtacgccgag cccgggacgg atctgggggc
     1321 ggcctggcgg gccaagctcg cccgagaggg gaagctggcc ccgacaggtg gctccgtccg
     1381 cgagctgatt ctcggtcaag gcgagtagcc ctgaccaaga ccggccacct cccacccaca
     1441 ggcggtcagc tgtgagcgac gacccaacac ccacatcgtc ggccggcgtc gccgagctca
     1501 ccgaacggct ccgtcggggc ggctacctgg ccgaccgagg gctcgccacc tcggtgtacg
     1561 tggcgatggc cctgggacgc ccgctcctcc tcgaaggtga ggtcggcgtc ggcaagaccg
     1621 agttggccaa ggtgctggcg acggtgttgg accgccgctt gatccggctt cagtgctacg
     1681 agggcatcga cacccaccag gcgctctacg agtgggacta cgcccgccag atgctccaga
     1741 tcagggcgtt gtcagagcac aacctcgccg acgaatcgca ggtggacaag ctcttcggcc
     1801 ccaagttcct cctcgagcgc ccgctgctga ccgcggtccg cgaaggtgac cgggccgtgc
     1861 tcctgatcga cgagattgac cgggccgacg atgaattcga ggcgttcctg ctggagctgt
     1921 tgtccgacta ccagatcacg attcctgaga tcgggacgat cgtcgccgag gcgccaccat
     1981 tggtgatcct cacctcgaac cgcacccgcg agctccatga cgccctgaag cggcgctgca
     2041 tgtaccactg ggtggggtat cccactacag aacgcgaagt ggagatcatc atggtgcgtg
     2101 cacctgaggc cggcgagact ttggctcgga aggtcgtcga ggcggtgcat cgtcttcgcg
     2161 ggctcgacct ggccaagccg cccggggtgg ccgagaccct cgattgggtg gcaaccctca
     2221 acctcctcga tagacccgac ctcgatgcaa gcaccgtgag agacaccctg ggtgccgtcg
     2281 tcaaggagcg cgacgacctc gagctggtac tcgagcatct cgaggaaatc gccgtcgatg
     2341 gttgagcagc ccagcaccct cgacctggtg gtcggcgacg agacgccctt catccggatg
     2401 ctggtcgatt tcggcgagcg gttgcgaaag gccggtttgc ctgtggggtc gggcgatctg
     2461 cttgcctact gcggcgccat ggcagcgctc aaccctggcg atctgctcga tctctattgg
     2521 gcaggccgga cgacgatggt cacccgccgc gaccacatcc cggtctacga ctcggtattc
     2581 cggagcttct ttctcgatag cgggggagac gttcccgaac ctctcgcact cgtgctcgag
     2641 caatcatcga tcgagaacca gtcggtgatc ga
//
LOCUS       DATITW010000447         1744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_382045, whole genome shotgun sequence.
ACCESSION   DATITW010000447 DATITW010000000
VERSION     DATITW010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382045"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..332
                     /locus_tag="VLS86_06245"
     CDS             30..332
                     /locus_tag="VLS86_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HSO50129.1"
                     /translation="MSELVIGITVFVLASFVGFEVITKVPPTLHTPLMSGANAISGIT
                     IIGALTAAGLGEGSLAQILGFVAVATATVNVVGGFLVTDRMLRMFRRPRDKGPGSP"
     gene            329..1696
                     /locus_tag="VLS86_06250"
     CDS             329..1696
                     /locus_tag="VLS86_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HSO50130.1"
                     /translation="MIEILYLICAVAFIIGLKRLSGPRTARSGNLLAGVGMLGTNVIT
                     LVAESIVNWGTVLAGLAVGTVVGDWAALRVKMTAMPEMVAAFNGLGGIASSLVAATAV
                     VESGLAGIPLETSVTILLSTAIGAVTFTGSFMAFGQLQGLVPGRPLQFPGQKFINGAL
                     GVGAIGVGIWTLTSGEQSGYWVVFGITLLIGVLAVLPIGGADMPVVISLLNSFSGLAA
                     AAAGFVIGNDALIIGGALVGAAGLILTVLMTEAMNRSLANVLFAGFGSGDGGGGGISV
                     SDRPVNRATAEDAAIALGYAGSVIIVPGYGLAVAQAQHAVRELALSLEERGATIHFAI
                     HPVAGRMPGHMNVLLAEADVPYEQLFDLEQADPQFSHTDVVLIVGANDVVNTSARDDP
                     GSPIFGMPILNVDRAATVIVIKRSLAPGFAGIDNPLFYDPKTMMFFDDAKEALEKTVV
                     ALREM"
BASE COUNT          255 a          569 c          590 g          330 t
ORIGIN      
        1 cgtcgcaacc tgaacctgga aggcacccca tgagcgagct cgtcatcggc atcactgtct
       61 tcgtgctcgc ctcattcgtt ggtttcgagg tgatcaccaa ggtgccgccc accttgcaca
      121 ccccactcat gtcgggggcg aacgccatct ccgggataac catcatcggc gccctcaccg
      181 ctgccgggct cggggagggc tcgctggccc agatcctcgg tttcgtggcg gtggccaccg
      241 ccaccgtcaa cgtggtcggc gggtttctgg tgaccgaccg gatgctgcgc atgttccggc
      301 gccctcggga caaggggccc ggtagcccgt gatcgagatc ctctacctga tctgcgcggt
      361 ggcgttcatc atcgggttga agcggctcag cggcccccgg accgcccgct ccggcaacct
      421 gctggccggt gtggggatgc tcggcaccaa cgtcatcacc ctggttgccg agagcatcgt
      481 caactggggc acggtgctgg ccgggctcgc cgtcggcacc gtagtcggcg attgggcggc
      541 actccgggtc aagatgacgg ccatgcccga gatggtggcc gccttcaacg ggctgggagg
      601 catcgcctcc tccctggtcg ccgctaccgc ggtggtcgag agcggattgg ccgggatccc
      661 gctcgagaca tcggtgacca tcctgctttc gactgcgatc ggcgccgtga ccttcaccgg
      721 cagcttcatg gctttcggac agctgcaggg gttggtcccg ggccggccgc tccagttccc
      781 ggggcagaag ttcatcaatg gcgcccttgg ggtgggggct atcggtgtcg ggatctggac
      841 cttgaccagc ggggagcagt ccgggtactg ggtggtgttc gggatcaccc tcctcatcgg
      901 cgtgctggcg gtgctcccga tcgggggggc ggacatgccg gtggtcatct cactgctcaa
      961 ctcgttctcc gggctggccg cagcggcggc gggcttcgtc atcggcaacg acgccctgat
     1021 catcggcggg gccctggtcg gggcggccgg gttgatactg accgtgctca tgaccgaggc
     1081 catgaaccgg tcgctggcca atgtgctctt tgctgggttc gggagcggcg atgggggcgg
     1141 cggtgggata tccgtctcgg atcggccggt gaatcgagcc accgccgagg atgccgcaat
     1201 cgccctcggc tacgccggat ccgtgatcat cgtccctggc tacgggctcg ccgttgccca
     1261 ggctcagcac gcggtgcgcg agctggcgct gtccctcgaa gagagggggg caaccatcca
     1321 cttcgccatc cacccggtgg cgggacgcat gcccggtcac atgaacgtgc ttctcgccga
     1381 ggcggacgtc ccctatgagc agctattcga tctcgagcag gccgatccgc agttctccca
     1441 cactgacgtc gtcttgatcg tcggggccaa cgacgtggtc aacacctcgg cccgcgacga
     1501 ccccggttcg ccgatcttcg ggatgccgat cctgaatgtc gaccgggcag cgacggtgat
     1561 cgtcatcaaa cgcagcctcg ctccgggatt cgccgggatc gacaaccctc tcttctacga
     1621 cccgaagact atgatgttct tcgacgacgc caaggaggcc ctggagaaga cggtggtcgc
     1681 cctgcgtgag atgtgagggc gatctctcgg attctgatcg gtgatcagac ggccgggtgg
     1741 gtcc
//
LOCUS       DATITW010000448         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_62135, whole genome shotgun sequence.
ACCESSION   DATITW010000448 DATITW010000000
VERSION     DATITW010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62135"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VLS86_06255"
     CDS             complement(<1..287)
                     /locus_tag="VLS86_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50131.1"
                     /translation="MQPKNVMYAAVGAPVVAARKVGEKVSDLRAKLSDEATGYGKTAE
                     KAIDSWAVEGEKVVGRLSEGKVVDELTSKVDLDQAKEQVSKLRDQLEDMLAT"
     gene            complement(291..656)
                     /locus_tag="VLS86_06260"
     CDS             complement(291..656)
                     /locus_tag="VLS86_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSO50132.1"
                     /translation="MNESKHTDLGAFIRQQRERANLSLRRLADTAGISNPYLSQIERG
                     IRKPSAEILSRLSRALEISANSLYSRAGLLDEDLQSPTISEAIDADGRLSSSQKKVLL
                     DMYRALIHSNGSDPHNEQE"
     gene            complement(743..1474)
                     /locus_tag="VLS86_06265"
     CDS             complement(743..1474)
                     /locus_tag="VLS86_06265"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HSO50133.1"
                     /translation="MTTEKETRPGPGPYEPHIGETRQYWRDIILGVNDGLVSIFLLVV
                     GVVGGGLNTEQVLLTALAGSVAGAISMAAGEYLATKSQDEVLEAELKLERVHIRDHRQ
                     QELDQLREMFTDMGIHDEDVETVVTAFDRSDDAILNAMKALEFGFVESERRSPYRAML
                     ASGVFFIAGSLPSIIPFLIFDTTSTAVIWATVLALAGLFLVGVVKARVAKHSWIRSGL
                     ENMAIAGAGGVLAWVIGDAAGTALI"
     gene            complement(1515..>2669)
                     /locus_tag="VLS86_06270"
     CDS             complement(1515..>2669)
                     /locus_tag="VLS86_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HSO50134.1"
                     /translation="ISAERLDELVEGIVPKAVELRRLLHRHPEPSTQEHQTTALIAAS
                     LDENGVGYHDRKPKTGLWLDIGPDPHIGFRADLDALPIDEPEGNAPRSQNQGWMHACG
                     HDAHAAIAFGMTLALHKLDLPVGVRVLFQPAEETFPGGAVELVEEGLVDGFKGIIAFH
                     IDPSLQTGTVGSRVGPITASADRMKIVLEGPGGHTARPHQTVDLVADAARLVHELPGV
                     LRRTLDARSPLTIAFGSIHGGRSGNVIPTRIALHGTARTLDRSLWESLPGLIDKAIGS
                     LMSVSGANYTLEYEQAIPPVVNDEAVVKAATDGIIGALGPEAVVPTEPSMGGEDFANY
                     LAVTPGALLRLGSSGRGNDLHSPGFVLDEGSIGFGVRAGVAAVAGLAEYL"
BASE COUNT          444 a          880 c          840 g          505 t
ORIGIN      
        1 gtggcgagca tgtcctcgag ttggtcgcgc agcttgctca cctgctcctt ggcctggtcg
       61 aggtcgacct tggaggtcaa ctcgtcgacc actttgcctt cggagagacg accgacgacc
      121 ttctcgcctt ctacggccca gctgtcgatc gccttctcgg cagtcttgcc gtatccggtc
      181 gcttcgtcac tgagcttggc gcgcaggtca ctgaccttct cgcccacctt gcgggcggcg
      241 accactgggg ccccgaccgc ggcatacatg acgttcttgg gttgcattgc ttactcctgt
      301 tcgttgtggg ggtctgaccc attggaatgg atcaaggctc tgtacatgtc gagcagcact
      361 ttcttctgac tcgagctgag acgaccgtct gcatcgatcg cctcggagat cgtcggcgat
      421 tgcaggtcct cgtcgaggag accagcccgc gagtagaggg agttcgccga gatctcgagc
      481 gcccgtgaca ggcgactcag gatctctgcc gacggcttgc ggatgccccg ctcgatctga
      541 gacagatacg ggttggagat ccctgccgtg tcggccagcc gacgtagaga gaggtttgct
      601 ctctcccttt gctgtcggat gaaagctccc aagtcggtgt gcttgctctc gttcactact
      661 tagaagcata ccgcaagctg ctaacaattg caagcagttg ctagcacgtc ttcgattggg
      721 agctgcaacc gagccgggac ggtcagatca gggctgtgcc cgctgcgtcg ccgatgaccc
      781 aggcgaggac cccacccgcc ccggcgatcg ccatgttctc cagtcccgac cgtatccagc
      841 tgtgcttggc gacccgcgcc ttgaccaccc cgacgaggaa gagaccggcg agggcaagga
      901 ccgtggccca gatcactgct gtgctcgtgg tgtcgaagat caggaaaggg atgatcgaag
      961 gcagcgaccc tgcgatgaag aagacccccg aagcaagcat ggcgcggtac ggcgacctcc
     1021 gctctgactc gacgaagcca aactcgagcg ccttcatcgc attgaggatc gcgtcatcgg
     1081 accggtcgaa tgcggtcacc acggtctcga cgtcctcgtc gtggatcccc atgtcggtga
     1141 acatctcccg aagttggtcg agctcctgct ggcgatggtc gcgaatgtgg acccgctcca
     1201 gcttcagctc cgcctcgagg acctcgtcct gggacttggt cgcgagatac tccccggccg
     1261 ccatcgaaat cgcccccgcc accgaaccgg cgagggccgt gagcaacacc tgttcggtgt
     1321 tcagcccgcc cccgaccact ccgaccacca gcaggaagat cgagaccagg ccgtcattga
     1381 ccccgaggat gatgtcacgc cagtactggc gggtctctcc gatgtgtggc tcataaggcc
     1441 cggggcccgg acgtgtctcc ttctcggtcg tcatcgatcg tgatggtacc tgcgaccccg
     1501 cggctccggc ctggtcagag atactcggcg agaccggcta ccgcggccac ccctgccctc
     1561 accccgaatc cgatcgagcc ctcgtcgagg acgaagccag gggagtggag gtcgttgcct
     1621 ctccccgagg aaccgagtcg gagaagtgct cctggtgtga ccgccaggta attggcgaag
     1681 tcctccccac ccatcgacgg ctcggtcggc accaccgcct ccgggcccaa ggccccgatg
     1741 atgccatcgg tcgccgcctt gaccaccgcc tcgtcgttga ccaccggcgg gatcgcctgt
     1801 tcgtactcca gggtgtagtt ggctcccgaa acagacatca ggctcccgat cgccttgtcg
     1861 atcagcccgg gcagtgattc ccaaaggctg cgatcgaggg ttcgcgccgt tccatggagc
     1921 gcgatccggg ttgggatcac gtttccggaa cgccccccat ggatcgagcc gaaggcaatc
     1981 gtcagtgggc ttcgagcgtc gagggtgcgt ctcagcactc cgggcagctc atgcacgaga
     2041 cgggccgcgt cggcgaccag gtcgacggtc tgatgtggtc gcgcggtgtg cccacccggg
     2101 ccctcgagca cgatcttcat cctgtccgcg ctggcggtga tggggccgac ccggctcccc
     2161 accgtgccgg tttggagcga agggtcgatg tgaaaggcga tgatcccttt gaatccgtcg
     2221 accaggccct cctccaccag ctcgaccgcg cccccgggga aggtctcttc agccggctgg
     2281 aacaggaccc ggactccgac cggcaggtcg agcttgtgga gcgccagtgt catgccgaag
     2341 gcaatcgcgg cgtgcgcgtc gtgaccacat gcgtgcatcc agccctggtt ctgggatcgg
     2401 ggggcattcc cttcgggctc gtcgatgggg agcgcatcga gatcggcccg gaagccgatg
     2461 tggggatcgg gcccgatgtc cagccacaga ccggtcttcg gcttgcggtc gtggtagccg
     2521 acgccgttct cgtccaacga tgccgcgatc agggctgtgg tctggtgctc ctgggtggac
     2581 ggctccgggt gacggtggag cagacgacgg agctccaccg ctttggggac gatcccctcc
     2641 accaactcgt cgagacgctc cgcgctgat
//
LOCUS       DATITW010000449         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_662478, whole genome shotgun sequence.
ACCESSION   DATITW010000449 DATITW010000000
VERSION     DATITW010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662478"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2328
                     /locus_tag="VLS86_06275"
     CDS             <1..2328
                     /locus_tag="VLS86_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSO50135.1"
                     /translation="LPPDEEGRMTTTRHRTLTNWGAYEVETDGHDITAVHPFAKDPDP
                     SPIGQSLKAVRKSRVERPAIRESWLRDGPGSGRERRGHEPFVEVEWDVALDLVAAELD
                     RVRSEYGNQAIFGGSYGWASAGRFHHAQSQIHRFLNSIGGYTYSVDDYSIAAGHVITP
                     HVFGFSFDDFMDEQPHFVEISENTELVVAFGGMPIKNSQVQYGGQGRHMLNSRLEACR
                     DRGVKFVNLSPLGEDLIETVEALWVPVRPSTDTALMLGLAHSLLVADQHNEGFLADYC
                     VGWDRLRSYLVGDSDGIPKTAEWAGAISGVDPDRIRSLAAEMAATRTLITVSWSVQRN
                     DHGEQPYWMAAALAAMLGQIGLPGGGVGYGYGAVGLNGHPVGYHALPRLSQGRNQVSD
                     FIPVARISDLLLHGGEEFDYNGQRLTYPETRLVYWAGGNPFHHHQDLNRLARAWQTPD
                     TTICHEPFWNALARHCDIVLPATTPLERNDVGGSGSDDFVFWMEQVIEPVAEARNDYD
                     IFSELARRLGSGEKFTEGRTAEAWLDHLYTRFRSNHPDYPTLDELKEAGHFQIPEEWI
                     PPSGSQLVAFRADPVGAALKTPSGRIELYSETLESFGYDDCPPHPSWLEPSEWLGNAD
                     RYPLHLISNQPKTRLHSQWDHGETSLAGKVDGREQLGMSPADARARALSHEDLVRVFN
                     DRGACLASLAIREDLMEGVVQLPTGAWWDPTEPGGLCRAGNPNVLTRDVGTSKLAQGP
                     TAQTCLVEVERFEGEAPPVLAHQHPELLADSTRAG"
BASE COUNT          431 a          803 c          798 g          399 t
ORIGIN      
        1 cttccaccgg acgaggaagg gcgaatgacc accaccagac atcggaccct gaccaactgg
       61 ggtgcctacg aggtcgaaac cgacggccat gacatcacgg cggtgcatcc gttcgccaag
      121 gatcctgatc cttcgcccat cggccagtca ctgaaggcgg ttcgcaagag ccgtgtagag
      181 cgacccgcaa tcagggaatc gtggctccgg gatggcccgg gctcgggtcg ggagcgtcgc
      241 gggcacgagc cgttcgtcga ggtcgaatgg gacgtcgccc tcgatttggt ggccgccgaa
      301 ttggatcggg ttcgatccga gtatggcaac caggccatct tcggtggcag ctacggctgg
      361 gccagcgccg gccgattcca tcacgcccag agtcagatcc accgcttcct caactcgatc
      421 ggcggctaca cgtactcggt agacgactac tcgatcgccg ccggccacgt gatcacaccg
      481 catgtcttcg ggttctcctt cgacgacttc atggacgagc agccccactt cgtcgagatc
      541 tcggagaaca ccgagctggt ggttgctttc ggtggcatgc cgatcaagaa cagccaggtc
      601 caatacgggg gccagggccg ccacatgctc aacagccggc tcgaagcgtg tcgggaccgc
      661 ggggtcaagt tcgtcaacct gtcgcccctc ggtgaggatc tgatcgagac cgtggaagcg
      721 ttgtgggtgc cggtgcgccc gagcaccgac accgcgctga tgctgggcct ggcccacagc
      781 cttctcgtcg cagaccaaca caacgagggc ttcctcgccg attactgcgt cgggtgggat
      841 cggctgcgct cctacctcgt cggagactcg gacggcatcc cgaagaccgc ggagtgggcg
      901 ggtgcgatca gcggagtgga cccggatcgg atccggtccc tcgccgccga gatggcggcg
      961 acacggacct tgatcacggt gagttggtcg gtgcagagaa acgaccacgg ggaacagccg
     1021 tactggatgg ccgcagccct ggccgcgatg ctaggtcaga tcggcctccc cggtggtggt
     1081 gtcgggtacg gctacggggc ggtcggcctc aacgggcacc cggtgggata tcacgctctc
     1141 ccccgtctct cccaggggag aaatcaggtg tccgatttca ttcccgtggc caggatctcc
     1201 gatctcctcc tccatggcgg ggaggagttc gactacaacg gtcaacggct cacctatccc
     1261 gagacccgcc tcgtctattg ggcggggggg aacccgttcc accatcacca ggacctcaac
     1321 cggctggccc gggcgtggca gaccccggac acgaccatct gtcacgagcc gttctggaat
     1381 gccctcgccc ggcattgtga catcgtgctc ccggccacca ctccgctgga gagaaacgac
     1441 gtgggtggga gcggcagcga cgacttcgtc ttctggatgg agcaggtgat cgaaccggtg
     1501 gccgaggcga ggaacgacta cgacatcttc tccgagctgg cccgacgtct tggcagcggc
     1561 gagaagttca ccgaaggacg gaccgccgag gcgtggctcg atcacctcta caccaggttc
     1621 cgctccaacc acccggacta cccgacactc gatgagctga aagaggcggg ccacttccag
     1681 attccggagg agtggatccc gccttcgggc tcacagctgg tggcattccg ggctgaccct
     1741 gtcggcgcgg cgctgaagac cccatcgggt cggatcgagc tgtactcgga gacgctcgag
     1801 agcttcggat acgacgactg cccgccgcac ccgtcgtggc tggagccgtc tgagtggctg
     1861 gggaacgccg accggtaccc gcttcacctg atctcgaacc agcccaagac ccgtctccat
     1921 tcgcaatggg atcacgggga gaccagcctg gccggcaagg tggacggtcg cgagcagctg
     1981 ggcatgagcc ccgccgatgc ccgggctcgg gccctgagcc acgaagacct ggtccgggtg
     2041 ttcaacgacc ggggtgcctg cctggcatcc ctggcgatca gggaggacct gatggagggc
     2101 gtggtccagt tgccgaccgg ggcctggtgg gaccccaccg agccgggtgg cctgtgtcgg
     2161 gcgggcaacc ccaacgtcct cacccgggac gtcggcacat cgaagctggc ccagggccca
     2221 accgcccaga cctgtctggt cgaggtggag cggttcgaag gggaggcccc tccggtgctg
     2281 gctcaccagc accccgagct cctcgccgac tcgacccgcg ccggctagac cctctcgatc
     2341 tcggacggca tctcgagggc gtgaccttca accgagatct tgggcaggat tcgatcgagc
     2401 cacttcggca gccaccagtt caggtcgccg a
//
LOCUS       DATITW010000450         1599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_141950, whole genome shotgun sequence.
ACCESSION   DATITW010000450 DATITW010000000
VERSION     DATITW010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141950"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VLS86_06280"
     CDS             complement(<1..326)
                     /locus_tag="VLS86_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50136.1"
                     /translation="MKNLDPEPGLTGPLRSRPDVAWIPDGMALFGWGERLRFDPGIGS
                     DRFDRARAAVAATSGSIGFTTFTFDEDASGSVVIIPETVLEVTARGTRVLTGQPSRIP
                     EPSPPSA"
     gene            complement(323..1099)
                     /gene="murI"
                     /locus_tag="VLS86_06285"
     CDS             complement(323..1099)
                     /gene="murI"
                     /locus_tag="VLS86_06285"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HSO50137.1"
                     /translation="MIGVFDSGVGGLGVLSHIRRELPSADLVYLADQARAPYGTRSLE
                     EVASIAEEVASWLLGRGATTVVVACNTASAAALSRLREMHPSIPFVGMEPAVKPAAIA
                     TKSGVIGVLATAATFQGELYSSVVSRHAPDVQVINAACPTWVRLVEAGQISGREVEQH
                     VARCLAPLVEGGADVLVLGCTHFPFLRPVIEQLVGTDVTVVDPAPAVALQVARVHRDT
                     EPGAGDLVLVTTGQPHHLASLARDLAGIEPTRPVLAWSSE"
     gene            1169..>1599
                     /locus_tag="VLS86_06290"
     CDS             1169..>1599
                     /locus_tag="VLS86_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSO50138.1"
                     /translation="MIEGSVAIVTGGVSGLGFGAAKRMVAAGGRVVMLDLNETRGAQA
                     ATELGDHARFIHCDVRDPANVVAAVTGAVESFGEINVLVNAAGVAPAQRVVARDGSLF
                     DLDLFRFVIDVNLIGMFDVIRNVAGAMAGNEPGESGERGVIV"
BASE COUNT          266 a          510 c          563 g          260 t
ORIGIN      
        1 gcggaaggag gggacggctc ggggatgcgg ctcggttgac cggtcaacac ccgggtgccc
       61 cgggcggtga cctcgagcac ggtctcgggg atgatgacca ccgagccaga cgcgtcttcg
      121 tcgaacgtga aggtggtgaa accgatgctg cccgaggttg ccgccaccgc agcgcgggca
      181 cggtcgaagc ggtcactccc gattcccgga tcgaaacgca ggcgctcgcc ccagccgaac
      241 agcgccatgc cgtcggggat ccaagcgaca tccggccggg atcgaagggg accggtcaat
      301 cccggttcgg ggtcgaggtt cttcattcgg aagaccaggc taggacaggt ctggttggtt
      361 cgattccggc gagatccctc gccaggctcg ccaggtgatg cggctggccg gtggtcacca
      421 ggaccaggtc ccctgctcct ggctccgtgt cacggtgaac acgggccacc tggagggcga
      481 ccgccggcgc cgggtcgaca accgtgacgt cggttccgac cagttgctcg atcaccggcc
      541 gcagaaacgg aaagtgggtg cagccgagca ccaggacatc ggctccccct tcaaccaggg
      601 gggcgaggca ccgggccacg tgctgctcca cctctcgccc gctgatttgc cccgcctcca
      661 cgagacgaac ccaggtgggg caggctgcgt tgatgacctg gacatcggga gcgtgtctgc
      721 tcaccaccga ggagtagagc tcgccctgga acgtggccgc ggtggccagc accccgatga
      781 cacccgattt ggtggcaatc gccgccggct tgaccgcggg ctccattccc acgaatggga
      841 tcgacggatg catctcccgg agccggctga gtgcagcggc ggaggcggtg ttgcaggcca
      901 ccaccacggt ggtggcacct ctgcccaaca gccagctggc aacttcctcg gcgatggacg
      961 ccacctcctc gagagagcgg gttccgtacg gggcccgtgc ctggtcggcg agatagacca
     1021 agtcggcgct gggaagctcc cgtcgtatgt gggagaggac tccgagccct ccgacaccgg
     1081 agtcgaacac gccgatcatg cgacctatct cagcaggctg cggcccgggt cactggtagc
     1141 gtcgcccgtc gtcacaggag ggcaattgtt gatcgaaggc tcggtcgcga tcgtcaccgg
     1201 aggagtatcg ggcctcggct tcggggccgc caaacggatg gtcgccgctg gcggtcgggt
     1261 ggtgatgctc gacctcaatg agactcgtgg tgcgcaggct gccaccgagt tgggcgacca
     1321 cgctcgcttc atccattgcg acgtgcgcga cccggcgaac gtcgtggctg cggtcactgg
     1381 cgcggtcgag tcgttcggcg aaatcaacgt cctggtcaac gcggcgggtg tcgcccccgc
     1441 gcagcgggtc gtggctcgcg acggctcgct attcgacctc gatctctttc gcttcgtcat
     1501 cgacgtcaac ctgatcggga tgttcgatgt gatcagaaat gtggcggggg cgatggccgg
     1561 caacgaaccg ggggagtcgg gcgagcgagg ggtgatcgt
//
LOCUS       DATITW010000451         1740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_62224, whole genome shotgun sequence.
ACCESSION   DATITW010000451 DATITW010000000
VERSION     DATITW010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62224"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VLS86_06295"
     CDS             complement(<1..898)
                     /locus_tag="VLS86_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50139.1"
                     /translation="MASLVQVVAIAPAQAVAGENGPAPIPGANTSGLSGSQWETKNQH
                     RIWWNADQTRWDAILPTNTGWRIAKGAIPETFGAAPVYGTNVGLLASNDDRPDFFWDD
                     TANRLYVLMSGESTTQFYALDYTPGTDTYSAPTTAVNLNGMEAPDSRASIFKSSNGDL
                     WASVMDEGGLLVSRSVNDGVTWSATPANLNFVIAEGQTQLTEFSDGGTFLGLAAAEDG
                     DDEREKGRFSQYMFYRIDLANAANFAAVVRATGTLTMTQVPLPGQTVNIHGEVYTFEA
                     AQISDTERTVAIVPGNLVATRNK"
     gene            complement(1098..>1740)
                     /locus_tag="VLS86_06300"
     CDS             complement(1098..>1740)
                     /locus_tag="VLS86_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50140.1"
                     /translation="RLTVGAQGEDRMTRGAARLSRSSASPLWRASYARSTDRFSGLAR
                     FLGANPQTLEAVLAGQRSRADRDLRQDLVLGWVLNLMARYQLATESDEDDWLVIDEGF
                     YQRGVALFSHGFVPLDLPLLNAYLDSIPQPEALVVVDTPLPVCEQRLERRGWSERVRH
                     LPIEGRRSFLADAAAATKAITDRLQTSGQGLIWVDGTTPVEDSLQTVAATLRS"
BASE COUNT          295 a          534 c          610 g          301 t
ORIGIN      
        1 gcttgttacg ggtggcaacg aggttgcccg ggacgatggc cacggtccgc tcggtgtcgc
       61 tgatctgcgc ggcctcgaag gtgtagacct cgccgtggat gttgaccgtc tggcccggga
      121 gggggacctg agtcatggtc agggtcccgg tggcccggac cactgcggcg aagttggcgg
      181 cgttggcgag atcgatccga tagaacatgt actgggagaa gcggcccttc tcgcgctcgt
      241 cgtcgccgtc ctcggcggcg gcaagaccga ggaaggtgcc accgtcggag aactcggtga
      301 gctgggtctg gccttcggcg atgacgaagt tcaggttggc cggtgtcgcc gaccaggtga
      361 cgccgtcgtt gaccgagcgc gacacgagga gtccgccctc gtccatgacc gatgcccaga
      421 ggtcgccgtt ggatgacttg aagatggagg cgcgggagtc gggagcttcc atcccgttga
      481 gattgaccgc agtggtgggg gcgctgtagg tgtcggtgcc cggtgtgtag tcgagggcat
      541 agaactgagt ggtgctctca ccgctcatca ggacgtagag ccgattggcg gtgtcgtccc
      601 agaagaagtc gggccggtcg tcgttggagg cgaggagacc gacgttggtg ccgtagaccg
      661 gtgccgcccc gaaggtctcg gggatggcgc ccttggcgat ccgccagccg gtgttggtgg
      721 gaaggatggc atcccatcgg gtctgatccg cgttccacca gatgcgatgc tggttcttgg
      781 tctcccactg gctaccggac aggccggagg tgttggcgcc gggaatcggc gccgggccgt
      841 tctcacccgc cacggcctgt gccggtgcga tggctaccac ctgcaccagc gacgccacca
      901 gcgcgaacgc cagaagcgca aaagaaaatc gtgtaccccc agccggagtg atccggttac
      961 ccatgtgaat gtctccctgc ccagttagaa gaagccccac agcttctgtc tgccgatact
     1021 aaagcgaatc ggtgaccacg caaagtggtt atccgactat tttccgtgac cggatctccc
     1081 tcagggcgat tcaccggtca cgaccgcaac gtagcagcga ccgtctgcaa tgagtcctcg
     1141 accggggtag ttccatcgac ccatatcagg ccctggcccg aggtctggag acgatcggtg
     1201 atcgccttgg tggcggccgc ggcgtcggcg agaaaggagc ggcgtccctc gatcgggagg
     1261 tggcgcaccc gctccgacca gcctcttctc tcgagtcgct gctcgcagac cgggagcggg
     1321 gtgtcgacca cgaccaacgc ctcgggctgt gggatcgagt cgagataggc gttgagcaat
     1381 ggcaggtcga gggggacgaa accgtgggag aacagggcca ctccccgctg gtagaacccc
     1441 tcgtcgatca ccagccagtc gtcctcgtcc gattcggtcg ccagttggta acgagccatc
     1501 aggttcagca cccaccccaa gaccaggtcc tgtctcaggt cccgatccgc ccgggagcgt
     1561 tgtccggcga gcaccgcctc cagggtctgg ggattggcgc cgagaaaccg ggccagcccc
     1621 gagaagcggt cggtggaccg ggcgtaggag gcgcgccaca atgggctggc gctggatcgg
     1681 gagagccggg ccgcaccccg cgtcatccgg tcctcgccct gggcccccac cgtgaggcgg
//
LOCUS       DATITW010000452         1910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_302201, whole genome shotgun sequence.
ACCESSION   DATITW010000452 DATITW010000000
VERSION     DATITW010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302201"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VLS86_06305"
     CDS             complement(<1..193)
                     /locus_tag="VLS86_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HSO50141.1"
                     /translation="MKLLAIETATPGASVALVEDATTLAAASRIDRLGHATFLVPAID
                     FCFDQVGWSPSDLDAIAVDI"
     gene            complement(190..642)
                     /gene="tsaE"
                     /locus_tag="VLS86_06310"
     CDS             complement(190..642)
                     /gene="tsaE"
                     /locus_tag="VLS86_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HSO50142.1"
                     /translation="MIEAHCPTEAETRAVARKLAPLLRPGDVVVLAGGLGAGKTLFTG
                     GLAAGLGVEETVVSPSFVLVRQYRSGFLPIVHVDVYRLGSLNEFDDLDVFDLASNGVL
                     VIEWGDALEAVIPEDHLKVEFVVEPDSARVLRLVPSGSWIDRDLESVA"
     gene            complement(639..1271)
                     /locus_tag="VLS86_06315"
     CDS             complement(639..1271)
                     /locus_tag="VLS86_06315"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSO50143.1"
                     /translation="MSRNIQVLQELETRALVCTQCGLAETRTQVVFGVGDPEARLMFV
                     GEAPGKNEDLQGEPFVGAAGKLLDSLLEEIDLKRSEVYIANVLKCRPPGNRDPRPDEI
                     DACKGYLREQIRLVAPEVVMTLGNFATKLLLNTEMGITRLRGQAYPWWLDAVLVPTFH
                     PAAALRSGDRVTSQMRDDFALVRSILDRAPEPSGTIPETPTDAAQMELFG"
     gene            complement(1294..>1910)
                     /locus_tag="VLS86_06320"
     CDS             complement(1294..>1910)
                     /locus_tag="VLS86_06320"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HSO50144.1"
                     /translation="GHVTPVIPVPIVPAAVVFDLQIGDPSVRPGPDHGEAAYRAATSE
                     PVPSGLVGAGTGTTVAKWRGFEHMQPGGLGSAIRAAGESMVGALVVLNAVGDIFTIEG
                     EPLTGGDHEPGPPNLIPESIANTTLVVLATDAAMARAELSRLAVRAHDALAACIRPVH
                     TRFDGDIAFAVSCGGGEGDVETLAEAAFGATAAAVERAVRGSAL"
BASE COUNT          330 a          658 c          638 g          284 t
ORIGIN      
        1 cgatgtcgac tgctatcgcg tccaggtcgg aaggtgacca gccgacctga tcgaaacaga
       61 agtcgatcgc cgggaccagg aacgtggcat ggccaagacg atcgatccgg gaggcagcgg
      121 ccagcgtggt ggcgtcctcg accagggcca ctgaagcgcc gggggtggcg gtctcgatgg
      181 cgagcagctt catgcgaccg actcgagatc tcggtcgatc caagatccgc tgggcacaag
      241 gcggagcacc cgggccgagt cgggctcgac cacgaactcc accttgaggt gatcctcggg
      301 gatgaccgcc tccagggcgt ccccccactc gatgaccagg acgccgttgg aggccagatc
      361 gaacacatcg aggtcgtcga actcgttgag cgacccgagc cggtacacat cgacatggac
      421 gatggggaga aagccggatc gatactgtcg caccaggacg aatgacgggc tgaccaccgt
      481 ctcttccacg ccgaggccgg ctgccaaccc gccggtgaac agcgtcttgc cggcgcccag
      541 gccacctgcg agcacgacca catcaccggg tcggagcagc ggggcgagct tgcgggcgac
      601 ggcccgcgtc tcggcctcgg tggggcaatg ggcttcgatc atccgaagag ctccatttgg
      661 gccgcgtcgg tcggcgtctc cgggatcgtc ccgcttggct cgggggcccg gtcgagaatg
      721 gaccggacca acgcaaagtc gtccctcatt tgcgatgtca cccgatcacc gcttcgcagc
      781 gccgccgccg ggtggaaggt cggaaccagc acggcatcga gccaccatgg gtaggcctgg
      841 ccccggagcc tggtgatgcc catctcggtg ttgagcagca gcttggtcgc aaagttgccc
      901 agggtcatga ccacctccgg cgcgacgaga cggatctgct ctctgagata gcccttgcag
      961 gcgtcaatct catcgggtcg cgggtcccgg ttgccgggag gacggcattt caggacattg
     1021 gcgatgtaca cctcggaccg cttgagatcg atctcctcga ggagcgaatc gaggagcttc
     1081 cccgccgcac cgacgaacgg ctcgccctgg aggtcctcgt tcttccccgg cgcctcgccc
     1141 acgaacatca gccgcgcctc ggggtcgccg acaccgaaca cgacctgggt tcgggtctcg
     1201 gccagtccac attgggtgca gaccaaagcg cgggtctcga gctcctgaag aacctggatg
     1261 ttccggctca cgccggccat tgtgccaaac cgatcagagg gcggagccac ggacggctct
     1321 ttccaccgcg gcggccgtgg ccccgaatgc cgcctcggcg agcgtctcca catcgccctc
     1381 gccgccacca caggacaccg cgaaggcgat gtccccgtcg aaacgggtgt ggaccggccg
     1441 gatgcaggcc gcaagtgcgt cgtgggccct gaccgccagc ctgctcaact cggcccgcgc
     1501 catggcggca tcggtggcca ggaccacgag cgtggtgttg gcaatcgact ccgggatcag
     1561 attgggagga cccggctcgt ggtcgccacc ggtgagcggc tcgccctcga tggtgaagat
     1621 gtcgccgacc gcgttgagga ccaccagggc gccgaccatg ctctccccgg ccgcccggat
     1681 cgccgagccc agccccccgg gctgcatgtg ctcgaagccg cgccacttgg cgaccgtggt
     1741 cccggtcccg gctcccacca aaccggacgg gacgggctcg ctggtggcgg cccggtacgc
     1801 ggcctcccca tgatctggcc cgggcctcac cgaaggatcg ccgatctgca ggtcgaaaac
     1861 cacggccgcg ggcacgatcg ggaccggaat caccggggtg acgtggcccc
//
LOCUS       DATITW010000453         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_181921, whole genome shotgun sequence.
ACCESSION   DATITW010000453 DATITW010000000
VERSION     DATITW010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181921"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VLS86_06325"
     CDS             complement(<1..885)
                     /locus_tag="VLS86_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSO50145.1"
                     /translation="MVVGSGAAGSSAAIAAARAGASTLLIEKLPFLGGTSTAVLDTFY
                     GFYTPGERARKVVGGVGDDVIAELRALGPVLERPNTYGAGTGVTYLAEHLKVVWEDLV
                     TGAGASVLLHGFVQEAEVADGRVSALVVATKSGLRRVRARVFVDASGDADVCHHAGFA
                     YEIAGEHDPAQTLTTTFRMSNVNHERRRTVDKSTLHALMEAAAESGEYDLPRREGSDH
                     VTPIDGVTATVMTRIDHVERDEAGRVKNATDPEILTRVEIRGRRQALDYARFLVDRVP
                     GYESASLVALSTQIGVRET"
     gene            complement(938..1852)
                     /locus_tag="VLS86_06330"
     CDS             complement(938..1852)
                     /locus_tag="VLS86_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HSO50146.1"
                     /translation="MNLGLLTAPFPGTPLNEVADWTATNGFTTIEIACWPASSGDTRR
                     YAGTSHIDVDGITDDEASEVADSITGCGLEISGLGFYPNPLHPDPEHRRAVIGHLKKV
                     IVAAGKMKVPVVNTFIGADSSKSQDENWEEATRVWPEIISCAEDNGVRIAIENCPMIF
                     SRDEWPSGHNLAYNPRIWRRMFEEFGETIGLNFDPSHLVWLMIDIESAIEEFGERFYH
                     FQAKDVMIDYDGLYENGTLSSGIGWQVPRLPGLGHVDWGVVFSALYRAGYDGPIIIEH
                     EDRGFEATDELVKRGFLLARDVLRPYVH"
     gene            complement(1854..3017)
                     /locus_tag="VLS86_06335"
     CDS             complement(1854..3017)
                     /locus_tag="VLS86_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSO50147.1"
                     /translation="MRFNEITAGVVGVGFIGVAHVEALRRLGVNVAGVVGSTPERAAI
                     KAEAANLPTVYESVEALAADANIDVVHIASPNYAHADQVRVCLAAGKHVVCEKPLALT
                     AEDTTDLVARAGAAGLVNAVCFNIRFYPLNHQAMAMVRAGAIGEPRLITGSYHQDWLL
                     LDSDWNWRLQPEDAGSLRAVADIGSHWLDLTRFISGRRIEEVMADLHTLVPIRRHPVG
                     PVESFVAVGSDQDLVEEKMTSDDAAGILLRYEGGARGAVTISQVSAGRRNAVRYEIAG
                     SQSALSWSSDAPDDLWIGNRGRPNEILHRDPSLAAPEAAALIAYPGGHVEGFPDTFRA
                     LFSRVYLDVIEGRPSDPPAYPTFADGHDAVLVTEAVARSNQEQRWAKVDRGGG"
     gene            complement(3020..>3683)
                     /locus_tag="VLS86_06340"
     CDS             complement(3020..>3683)
                     /locus_tag="VLS86_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO50148.1"
                     /translation="LAVGVVAGAINGVGVGVFRVNPLIMTLAMSAILLGLFTALSFTV
                     LQGSTSVAPFIKTLGGGSFLNNLIPYNAVVWAGVSLVIIYGLRRTGLGRLLYAVGDNP
                     VAVRLAGVRIWQVQIAAYTIAGLLAALAGILLAGRTGAVDLQLAVTFLLPSVAAAVIG
                     GTSIFGGVGSYSGTIFGALILGVLATMLTFLGAGQAVQQIVYGSIVLGLAWLYANVTG
                     SD"
BASE COUNT          700 a         1220 c         1131 g          632 t
ORIGIN      
        1 cgtctccctc accccaatct gggtgctgag ggcaaccagc gacgccgact cgtagccggg
       61 gacgcgatcg accagaaacc gcgcatagtc gagggcttgg cgtctccccc ggatctcgac
      121 gcgggtcagg atctcgggat cggtggcgtt cttcaccctc cccgcctcgt cccgctccac
      181 atggtcgatg cgggtcataa ccgtcgccgt gactccgtcg atcggggtca cgtgatcact
      241 cccctcccga cggggcaggt catactcgcc cgattcggca gcagcttcca tcagggcatg
      301 aagcgtcgat ttgtccacgg tgcgacggcg ctcgtgattc acattcgaca tgcgaaaggt
      361 ggtggtcagg gtctgtgccg gatcgtgctc accggcgatc tcgtaggcga aaccggcatg
      421 gtggcaaacg tcggcgtctc ccgatgcgtc gacgaagacc ctcgccctca cccgtctcag
      481 gcctgatttg gtggcgacca ccagcgccga tacccggcca tcggccacct ccgcctcctg
      541 gacaaagcca tggagcagca cggaggcgcc ggctccggtc accagatcct cccagaccac
      601 tttgaggtgc tcggcgagat aggtgacccc ggtcccggcc ccgtaggtgt tcggccgctc
      661 cagcaccggg cctagggcgc gaagctcggc gatcacgtcg tcaccgaccc cgcccaccac
      721 cttcctcgcc cgctctccag gtgtgtagaa cccatagaag gtgtcgagga cggcggtcga
      781 ggtcccgccg agaaagggga gcttctcgat gagcaacgtg ctcgctccgg cccgggccgc
      841 ggctatcgcc gccgacgacc cggcagctcc ggagccgaca accacgacgt cataggggcc
      901 gagggtcaga ccgcctccct cgacgggctc cggcggctca atgcacgtag ggccggagca
      961 cgtccctggc gaggaggaag ccccgcttca ccaactcgtc ggtggcttcg aaaccgcgat
     1021 cctcgtgctc gatgatgatc ggcccgtcgt aaccggcccg gtagagagcg gagaagacca
     1081 ccccccagtc gacgtggccc aacccgggga gacgcgggac ctgccacccg atgccggatg
     1141 acagggtccc gttctcatag aggccgtcgt agtcgatcat cacatccttc gcctggaagt
     1201 gatagaagcg ctccccgaat tcctcgatcg cggattcgat gtcgatcatc agccagacga
     1261 ggtgggaagg atcgaagttg aggccgatcg tctccccgaa ctcctcgaac atccgacgcc
     1321 agatcctcgg gttgtaagcc aggttatggc ctgacggcca ttcgtccctg gagaagatca
     1381 tggggcagtt ctcgattgcg atcctgacac cgttgtcctc agcgcatgag atgatctcgg
     1441 gccagaccct ggtggcctcc tcccagttct catcctggct cttggagcta tcagcgccga
     1501 tgaaggtgtt gaccactggg accttcatct tccccgcggc gacaatcacc ttcttgagat
     1561 ggccgatcac agctcgccgg tgctcggggt ccggatggag cggattcggg tagaaaccca
     1621 gacccgagat ctcgagcccg cagccggtaa tgctgtctgc gacttccgag gcctcgtcgt
     1681 cggtgatccc atcgacatcg atatggctcg tcccggcgta gcgacgggtg tcaccactcg
     1741 aagccggcca gcaggcgatc tcgatcgtgg tgaaaccgtt ggtggcggtc cagtcggcaa
     1801 cctcgttgag cggggtgccc ggaaatggcg cggtcaacaa cccgaggttc atctcatcct
     1861 cctcctcgat ctactttggc ccagcgttgt tcctggttac ttctggccac cgcctcggtc
     1921 accaggaccg cgtcgtgacc gtcggcaaaa gtgggatatg cgggaggatc cgatggccgt
     1981 ccctcgatca cgtcgaggta cacccggctg aatagggccc ggaaagtatc ggggaatcct
     2041 tcgacatggc caccgggata ggcgatcagg gccgcagcct caggtgcggc gagggacggg
     2101 tcacggtgca ggatctcgtt gggacgcccg cgattgccga tccacagatc atcgggagcg
     2161 tcggacgacc aggacaacgc agactgactg ccggcgatct cataacgaac cgcgttccgc
     2221 ctccctgccg agacttgaga gatggtcact gcgccgcgcg ctcccccctc gtagcggagg
     2281 agtatcccgg cggcgtcgtc gctggtcatt ttctcctcga ccaagtcttg atcgctgcca
     2341 acggctacaa agctctccac cgggccaacc ggatgacgcc ggatcgggac cagagtgtgg
     2401 agatcggcca tcacttcctc gattcggcgg ccgcttatga aacgggtcag atccaaccaa
     2461 tgtgatccga tgtcagcgac cgcccggagt gaacctgcgt cttccggctg cagtctccag
     2521 ttccaatcgc tgtcaagcaa caaccagtcc tggtgatagg agcccgtgat caggcgaggc
     2581 tcgccgatgg ctccggcgcg caccatcgcc atcgcctgat gattgagcgg gtagaagcga
     2641 atgttgaagc acaccgcatt caccaacccc gccgctcccg ccctggcgac gaggtccgtt
     2701 gtgtcctccg cggtcaaggc gaggggcttc tcgcatacga catgttttcc cgcggccagg
     2761 cagacccgga cttgatcggc atgggcgtaa ttcgggctgg cgatatggac gacatcgatg
     2821 ttggcgtcgg ccgccaaggc ttcgaccgac tcatagacag tcggcaggtt cgctgcctct
     2881 gctttgatcg cggcccgctc gggcgtggac ccgactacac cggcgacatt caccccaagg
     2941 cgacgaagcg cctcgacgtg ggcgactccg atgaagccga cgccaaccac accggctgtt
     3001 atctcgttga atctcatggt tagtcgctcc cggtgacatt ggcgtacaac cacgccagcc
     3061 cgaggacgat tgacccgtag acaatctgct gcacagcctg accggcgccg aggaaagtca
     3121 gcatcgtggc caggaccccc aggatcaggg cgccgaaaat ggtgcccgag taagagccca
     3181 ctcctccgaa tatcgaggtc cccccgatca cggcggcagc caccgaggga aggagaaagg
     3241 tcactgccaa ctgcaggtct acggctccgg tcctcccagc gagaaggatg ccggcgaggg
     3301 ccgctagaag cccggcgatg gtataggccg ctatctgcac ctgccagatc cgcacgccgg
     3361 ccagacggac cgcgaccgga ttgtccccga ccgcatagag gaggcgaccg agacccgtgc
     3421 gccgcaaccc atagatgatc actagcgaca cgccggccca gaccactgcg ttgtatggga
     3481 tcaagttgtt gaggaaggag cccccaccca gggtcttgat gaatggagcc acggaggtgg
     3541 aaccctgaag gacggtgaag gagagggcgg tgaacaggcc gaggaggatc gccgacattg
     3601 ccagggtcat gatcaggggg ttgacccgga acaccccgac ccccacccca ttgatcgccc
     3661 ctgcaaccac cccgaccgcg agg
//
LOCUS       DATITW010000454         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_583111, whole genome shotgun sequence.
ACCESSION   DATITW010000454 DATITW010000000
VERSION     DATITW010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583111"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="VLS86_06345"
     CDS             complement(<1..1336)
                     /locus_tag="VLS86_06345"
                     /inference="COORDINATES: protein motif:HMM:NF023650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HSO50149.1"
                     /translation="MAIGAVVMLLLVSYGVTRYVSRGEIMGRVEVAGTQIGGLDEERA
                     LTALIVVEEEYLRRPAVFNIEGKFVSLDPPEAGLDIDEQTITDHAMQVGRGGNVVSEF
                     VWWLTHIFDTVQLPVRGAIDEVAIEAVFDGWDSEVIALPNSPGGVTLEGGQPVPQYPK
                     VGTGVDRPAAAAVVEATLLALEPDRTTIPTATIVPELTDEDVDLAVAQAETLLAGPIQ
                     LSHVDTVAEFTVEQLTEAFRSETITEGEARIVLSFDVETIDGYLDEVRDQVEAEPVNA
                     VFEISGDSIAIVPGANGTEIDAAETARRLVDAGRTSARAGVLPLIEAAEPEVTTEYLE
                     SLNIQHLVSQFTTYHECCQERVTNIQTMADAVDKTIVLSGQTFSLNDHVGERTVEKGY
                     LPAGTIIAGELEDTVGGGVSQFTTTLYNAVFWGGYEDVEHKPHSYYFSRYPEG"
     gene            1489..2265
                     /locus_tag="VLS86_06350"
     CDS             1489..2265
                     /locus_tag="VLS86_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HSO50150.1"
                     /translation="MTIQFPPVVEGEAIRVSATTYVTYKKCPGQAGARLQGIYGPDSR
                     PAFLGSLAHRIFSRHLTSGPISSEEFVQACREEIGGSTLNNKLAGLEMRPSALAAAIE
                     EVRGLYERFTKFPNDGFEGSEFSIESTPATGVDLVGTVDAVYREDLGGHRLVDWKTGE
                     LGEPEDQLMFYALLWLLHGGELPAYVEAVSVRTGERYRTVPSSADMQRVVEELGDLVN
                     TIRAAWASGDDLVRSGGPWCHFCPILSDCDEGQSAEALLE"
     gene            complement(2283..2843)
                     /locus_tag="VLS86_06355"
     CDS             complement(2283..2843)
                     /locus_tag="VLS86_06355"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HSO50151.1"
                     /translation="MPETRGPVLALLRGINVGGRSMLSMSELRAALDAAGLPGARTHL
                     QSGNLLVTPAAGKVRGLAGTIRDVIKGDFGLDIQVLLRTRPELDEIVARNPMTGPEAT
                     GSKVHVVFLASTPEAKRVDDLDPDRSPPDRFVVAEREIYVHYPNGSGRSKLSLDYFEK
                     RLGVVGTARNWNTVTRLLEMLDESPE"
     gene            complement(2836..3714)
                     /locus_tag="VLS86_06360"
     CDS             complement(2836..3714)
                     /locus_tag="VLS86_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006008648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSO50152.1"
                     /translation="MTEQRRIVLDGVATEVLRDGEDLVAADGRRTSIQSAVHLPPVQP
                     SKILAVHLNYRSRVEEFMTRLPSAPTYFHKPTTALNSHNGEVVRPLGCRFLNFEGEIA
                     IVIGTKCHDIAPADAAAHIAGYTIANDFGLHDFRDTDAGSMLRVKGSDTLCPVGPGLV
                     AGWDPRDKTIRTKVNGVIRQQGNTREMEWDMSYLVADLARTITLLPGDIILSGTPAGS
                     RPVEPGDVVTVEVDGLGALTNTIVDGHSLHTDVGAQPSDSEEVVSTALGGDWEHRGER
                     PPQGAGARHYQSELDA"
BASE COUNT          625 a         1233 c         1317 g          713 t
ORIGIN      
        1 ccccctcggg gtacctcgag aagtagtagg agtggggctt gtgctcgaca tcctcatacc
       61 cgccccagaa caccgcgttg tacagggtgg tcgtgaactg gctgaccccg cccccgacgg
      121 tgtcctcgag ctcgccggca atgatggtcc cggccggtag gtagcccttt tccacggtcc
      181 gctcgccgac gtgatcgttg aggctgaagg tctgaccgga gaggacgatg gtcttgtcca
      241 ccgcgtccgc catggtctgg atgttggtca cccgctcctg gcagcactcg tgataggtgg
      301 tgaactggga caccaggtgc tggatgttca ggctctcgag gtactcggtc gtcacctcgg
      361 gctctgcagc ctcgatcaag ggcaggaccc cggcccgggc gctggtcctg ccggcatcga
      421 ccaggcgacg ggcggtctcg gcggcatcga tctcggtccc attggccccg gggacgatgg
      481 cgatgctgtc gcccgagatc tcgaacaccg cgttcaccgg ctcggcctcg acctggtcgc
      541 ggacctcgtc gagatacccg tcgatggttt cgacatcgaa cgagagcacg atcctggcct
      601 ctccctcggt gatggtctcc gatcgaaagg cctccgtgag ctgctcgacg gtgaactcgg
      661 cgacggtgtc gacatgactc agctggatcg ggccggcgag gagggtttcg gcctgggcca
      721 cggccaggtc gacgtcctcg tcggtcagct cggggacgat ggtggcggtt gggatggtgg
      781 tgcggtcggg ctcgagggcg agcagcgtcg cctcgaccac ggctgcggcc gcgggacgat
      841 cgacaccggt cccgaccttg gggtattggg ggacaggctg gccgccctcg agggtcaccc
      901 cgcccgggct gttggggagg gctatgacct cggagtccca accgtcgaac accgcttcga
      961 tcgcgacctc gtcgatcgcc cctcgaaccg ggagctggac ggtgtcgaag atgtgggtga
     1021 gccaccagac gaactcggat acgacgttcc cgccccgccc cacctgcatg gcatggtcgg
     1081 tgatggtctg ctcgtcgatg tcgagcccgg cctcgggggg atcgaggctt acgaacttgc
     1141 cctcgatgtt gaacacggcc gggcggcgca ggtactcctc ttcgaccacg atgagcgcag
     1201 tcagggcgcg ttcctcgtcg agaccgccga tctgggtccc ggcgacctcg acccgaccca
     1261 tgatctcgcc ccgggacacg tatcgggtga ccccgtagga gaccagcaac aacatgacga
     1321 cggcgccgat cgccacggcg agcgcgctaa gcgacaaatt gcgtgtggag agatgcttgt
     1381 tcatgcaaag gtcgccccaa atcgaccttg agcccggtcg ccggatgcta acgaggtcgg
     1441 acgctctaca tgaagtggtg tctagggcac gtatacgctc gcagacgagt gacgattcag
     1501 ttcccgccgg tggtcgaagg agaggcgatc agggtctccg ccacaacgta tgtcacctac
     1561 aagaagtgcc cgggacaggc gggggcgcgg ctccagggca tctatgggcc cgacagccga
     1621 cccgccttcc tggggagtct cgcccatcgc attttcagca ggcacctcac ctcgggcccg
     1681 atctcatccg aggagttcgt ccaggcctgt cgcgaggaga tcggtggctc gacgctcaac
     1741 aacaagctgg ccggtctcga gatgcgaccc tcggcgctgg cggccgccat cgaagaggtc
     1801 cgtggtcttt acgagcgctt caccaagttt cccaacgacg gcttcgaggg ctcggagttc
     1861 tcgatcgaga gcactccggc gacgggtgtg gacctggtcg ggaccgttga cgccgtgtac
     1921 cgggaggatc tcgggggtca ccgtctggtc gactggaaga ccggggagct cggcgagccc
     1981 gaggatcaac tcatgttcta cgccttgctc tggttgctcc acggtggcga gttgcccgcc
     2041 tatgtcgagg ccgtctcggt gcggaccggc gagcgctatc ggacggtccc ttcctctgcc
     2101 gacatgcagc gggtggtcga agagctgggg gatctggtga acaccatcag agctgcctgg
     2161 gcctcgggcg atgacttggt gcgcagcggc gggccctggt gtcacttctg cccgattctg
     2221 tcggactgtg acgagggtca gtcggccgag gcgctgctcg agtgagcgaa gcgctttggg
     2281 tgtcactcgg gcgactcgtc gagcatctcc aacagcctgg tcacggtgtt ccaattgcgg
     2341 gcggtcccga ccacgcccag tcgcttctcg aagtagtcga gtgagagctt ggatcgacct
     2401 gagccgttgg gatagtggac atagatctcc ctctccgcaa ccacgaaacg atcgggtggc
     2461 gaccggtcgg gatcgaggtc atcgacccgt ttcgcctcgg gagtcgaggc cagaaacacg
     2521 acgtggacct tggatccggt cgcttccggc ccggtcatcg gattccgggc gacgatctcg
     2581 tcgagctcag gccgggtgcg gagaagcacc tggatgtcga gaccgaaatc gcccttgatc
     2641 acatcccgga tcgtcccggc caggccacgc actttcccgg ccgccggggt gacgagcagg
     2701 tttccgctct ggagatgggt gcgggctccg ggaagccccg ccgcgtcgag agccgctctc
     2761 agctcggaca tggagagcat ggatctgcct cccacgttga tcccgcggag gagggcgagc
     2821 accgggcccc gggtctcagg catccagctc cgattggtag tgcctggcgc cggcgccctg
     2881 cggggggcgc tccccgcgat gctcccaatc gcctccgagg gcggtggaga ccacctcttc
     2941 ggagtcgctg ggttgtgctc ccacgtcggt gtgcagcgag tgtccgtcga cgatggtgtt
     3001 ggtcagcgct cccagaccgt cgacctcgac ggtgaccaca tccccgggct cgacgggtcg
     3061 tgacccggcc ggcgtcccgc tgaggatgat gtcgccgggg agcaaggtga tggtcctggc
     3121 caggtccgcc accagatagg acatgtccca ttccatctcg cgggtgtttc cctgctgacg
     3181 gatgacgccg ttcaccttgg tcctgatcgt cttgtccctc gggtcccatc ctgcgacgag
     3241 gccggggccg accgggcaca gtgtgtccga ccccttgacc cggagcatgg agccggcgtc
     3301 ggtatcgcgg aaatcgtgga ggccgaagtc gttggcgatg gtgtagccgg cgatgtgagc
     3361 cgcggcgtca gcgggcgcga tgtcgtgaca tttggtcccg atcacgattg cgatctctcc
     3421 ctcgaagttg aggaagcggc atccaagggg tcggaccacc tccccgttgt gtgaattgag
     3481 ggcggtggtt ggcttgtgga agtaggtggg ggcagacggc agcctggtca tgaactcctc
     3541 gacccggctc cgatagttga ggtgcaccgc caggatcttc gacggctgca ccggagggag
     3601 atggaccgcg gattggatcg atgtccgacg tccgtcggcc gcgaccagat cctcgccgtc
     3661 acggaggact tcggtggcga cgccgtcgag aacgatcctg cgctgctcgg tcatctgtct
     3721 cacacgctac ccagggccat ttgcgattat gttcggttcc aaacgacaca tgactcagaa
     3781 cacgctccac gtcgatctcg gtgaggaagg cgccgacctg accgcgcacg acgccttcgt
     3841 ccgcgccgtg cctcacgcca ccttcgcccg catgcgacgc gagcagcc
//
LOCUS       DATITW010000455         1739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_342323, whole genome shotgun sequence.
ACCESSION   DATITW010000455 DATITW010000000
VERSION     DATITW010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342323"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VLS86_06365"
     CDS             complement(<1..700)
                     /locus_tag="VLS86_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50153.1"
                     /translation="MSRSLALARGVAVGCVASTLLFAVAVLVGLADTGSGVVFLTGAL
                     AGAGVGLSTRSDPSVEAIGPFSVGVVGLIAGLATGFGWLEFSVIGGAVVSGLWLIPGE
                     GHPSDPWRWFRWGGVAALIVLLILLPLIVDGGTLGHDESAYALKARQWLEGTPGTGWS
                     PHRATGMSAYGYVVLALGGSEAGLRLLGLTGTIALVAGVWALGNRLADPRVGAIASIA
                     VVAGPSVLYRSTEYL"
     gene            760..1686
                     /locus_tag="VLS86_06370"
     CDS             760..1686
                     /locus_tag="VLS86_06370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSO50154.1"
                     /translation="MTAGPSLDVIIPAAKAAGSIGDTIDSIKFQGYPNLGVIVVASHD
                     EETGEVARRHGAVVVPNPSGLTPSGLNLALAATTAEIVARVDAHSTLPAGYLQRAVAT
                     LLESGADNVGGMQIPIGTTGWEKAIAAAMASRFGAGDAKHRVGGGAGPVDTVYLGVFR
                     RATLERLGGYDETFARNQDYELNHRIRESGGVVWFDPELRVAYKPRGSLSELARQYFQ
                     YGQWKREFTRRHPGSLRWRQLAPPSLVALLIPALVTGLWWPPAWLLPAGYLSSLIIIG
                     LGKLPRLGVSALGVPPALATMHLSWGLGFLLR"
BASE COUNT          325 a          617 c          549 g          248 t
ORIGIN      
        1 ccaagtactc ggtgctgcga tacaggaccg agggcccggc gaccaccgct atcgaggcga
       61 tggctccgac tcggggatcg gccaagcggt tgcccaacgc ccacaccccc gcgaccagtg
      121 caatggtgcc ggtcaggccc agcagacgca gacccgcctc ggagccacca agagcgagga
      181 ccacgtaccc gtacgcggac atccccgtcg ctcggtgcgg cgaccagccg gtccccggtg
      241 ttccctccaa ccactgccga gccttcaggg cataggcaga ctcgtcgtgc cccagggtgc
      301 caccgtcgac tatcagcggc aggaggatca ggagaacgat cagggccgcg acgccccccc
      361 atcgaaacca gcgccacgga tccgaaggat gcccttcccc ggggatgagc cagagtccgg
      421 agacgacggc gccgccaatc accgagaact cgagccagcc aaacccagtg gccaggcccg
      481 caatcagccc aaccaccccc actgagaacg gccctatcgc ctcgaccgag ggatctgacc
      541 gtgtcgacag cccgactccg gcaccggcga gagccccagt gagaaagacc acgcccgatc
      601 ccgtgtcagc cagcccgacc aggacagcaa ccgcaaacag cagtgtggaa gcaacacagc
      661 cgactgccac acctctggcc agggcaagac tcctcgacat ggcggccacg ttacatacag
      721 cttgccgcag gcctgcaccg gccctgggaa gatgacccga tgaccgccgg accctccctc
      781 gacgtgatca tcccggcggc aaaggctgcc ggctcgatag gggacaccat cgactcgatc
      841 aagttccagg gctatccaaa cctgggagtg atcgtcgtcg ccagccacga cgaggaaacc
      901 ggggaggttg cgagacgtca cggagccgtg gtcgtcccca acccgagcgg tctgacacca
      961 tccgggttga acctggctct ggcggcgaca acggccgaga tcgtcgcccg ggtcgatgcg
     1021 cactcgacgc tccctgcggg ctatctgcag cgagccgtcg ccacccttct cgagtccggc
     1081 gcagacaacg tcgggggcat gcagatcccg atcggcacca ccggatggga gaaggcgatc
     1141 gcggccgcga tggcctcgcg tttcggggca ggagacgcca agcaccgggt gggaggcggc
     1201 gccgggccgg tggataccgt ctatctcggg gtcttccgcc gcgcgaccct ggaacgattg
     1261 ggagggtacg acgagacgtt cgcgcgcaac caggactacg agctcaacca ccgaatccgc
     1321 gagtcggggg gcgtggtgtg gttcgacccc gagttgcgcg tggcctacaa gcctcggggc
     1381 tccctgtccg agctcgccag gcagtacttc cagtatggcc aatggaagcg ggagttcacc
     1441 cgaagacacc caggctcgtt gcgctggcgc cagctggcac ccccatcgct ggtggcgctt
     1501 ctcatcccgg ccctggtcac cggtttgtgg tggcctccgg cctggctgct gcctgccggc
     1561 tatttgtcga gcctgatcat aatcggtctg ggcaagctgc cccggctcgg cgtctcggcg
     1621 ctgggggtcc cgccggctct ggccaccatg cacctcagct ggggtctggg gttcctcctg
     1681 aggtgagcaa ctggtcgtag aaggacatca catcctcctc gggccacctg aacctctcc
//
LOCUS       DATITW010000456         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_662744, whole genome shotgun sequence.
ACCESSION   DATITW010000456 DATITW010000000
VERSION     DATITW010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662744"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VLS86_06375"
     CDS             <1..1126
                     /locus_tag="VLS86_06375"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSO50155.1"
                     /translation="DLGAEVIKIEPHTGDTARNATIAPLQGESAIHLFMNRGKKSVVL
                     DLKDPRGHEMFLSLVEQADVVIDNFRPGVMARLGIDHDSLKQRNPDIITSSVTGFGEY
                     GPAKDKSAFDLVVQAYSGHVDITGPADGDPARVGIPIADIAGGIYSCISVLAALAGRE
                     LHGNGQHADVAMLDTLVSLLSYDALDHLNSGRKLTRLGTAHAHMVPWQAFQTSDGYVV
                     VAARDEKFWANLCDAIDRPDLKVDPRTVNNSERVAHRDYVVPILADVFRTKTNHEWLA
                     ILDEYDIPNAPVNDIEAVFTDPQVVARSMIGTYKHPTLGDIRYPPSPVKFSEWESPNL
                     PAPMLGEHTTEVLMQRLGLDESEVRELADTGVVKVWPEPS"
     gene            1137..2093
                     /locus_tag="VLS86_06380"
     CDS             1137..2093
                     /locus_tag="VLS86_06380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSO50156.1"
                     /translation="MRFGTFVPQGWKGDQDGVPVEKQWDRILDFAGLIEDAGYDSIWV
                     YDHFHTHPVVTQESTFEAWTLMAALAAVTTRVRLGQMCTCALYRPPSLLAKMAANIDV
                     ISGGRLDVGIGAGWSKGEFEAYGYRFPSDRQRLDMLEEAVQVLIAMWTQDEAGFQGEH
                     YSVDGAINRPHPIQKPHPPLWISGGGEKRTLRIVARYGDFANFGDNLEEFRHKSRVLA
                     GHCEAVGRPYEEIGRTIHRMSVIGRDEADLRRKLDIAAGRRSCAPEEFADEHFAATVP
                     QAVEEMGRFIDEGCADMILYFYDMGEADSLELFASEVIPQLG"
     gene            2090..>2277
                     /locus_tag="VLS86_06385"
     CDS             2090..>2277
                     /locus_tag="VLS86_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HSO50157.1"
                     /translation="MSDLDRLLDPATIAIVGLSAEPAKHGGRVLSNLRILGFEGQVWG
                     VNPRLPEVDGIDVFATVA"
BASE COUNT          413 a          736 c          749 g          379 t
ORIGIN      
        1 ggacctcggc gccgaggtga tcaagatcga gccgcacaca ggtgacaccg cgcgcaatgc
       61 gacgatcgcc cccctccagg gcgagagcgc gatccacctc ttcatgaatc gcgggaagaa
      121 gagcgtggtg ctcgatctca aggaccctcg cggccacgag atgttcctct ccctggtcga
      181 gcaggccgac gtggtgatcg acaacttccg acccggggtc atggctcgcc tcggcatcga
      241 ccacgacagt ctgaagcagc gcaacccgga catcatcacc tcctcggtga ccgggttcgg
      301 cgagtacggg ccggcaaagg acaagtcggc gttcgacctg gtggtccagg cctacagcgg
      361 ccatgtcgac atcaccggcc ccgcggacgg ggacccggca cgggtcggga tcccgatcgc
      421 cgacatcgcc ggaggcatct actcctgcat ctcggtcctg gccgccctcg ccgggaggga
      481 gctccacggc aatggccagc atgccgacgt ggcgatgctg gacaccctgg tctcgcttct
      541 ctcctacgac gcactcgacc acctcaactc ggggcggaag ctgaccagac ttggcaccgc
      601 tcacgcccac atggtcccct ggcaggcgtt tcagaccagc gacggatacg tggtggtggc
      661 ggcacgtgac gagaagttct gggccaacct gtgtgatgcg atcgaccgac ccgatctcaa
      721 ggtggacccg cggacggtca acaactcgga gcgcgtcgct catcgcgact acgtcgtccc
      781 catcctggcc gacgtgtttc gcaccaagac caaccacgag tggctggcga ttctcgacga
      841 gtacgacatc cccaacgccc cggtcaacga catcgaggct gtgttcaccg acccccaggt
      901 ggtggcccgg agcatgatcg gcacctacaa gcatccgacc ctgggggaca tccgctatcc
      961 cccgagcccg gtgaagttca gcgagtggga gagccccaac ctgccggcgc cgatgctggg
     1021 cgagcacacg accgaggtgc tgatgcagcg tctggggttg gatgagtccg aggtccggga
     1081 gctggccgac accggtgtgg tcaaggtctg gccggagccg agctgagggg atgaggatgc
     1141 gtttcgggac attcgtgcca cagggctgga aaggcgacca ggacggggtg cccgtagaga
     1201 agcaatggga tcggatcctc gatttcgccg gtctcatcga ggacgccggg tacgactcga
     1261 tctgggtcta cgaccatttc cacacccatc cggtggtcac ccaggagagc actttcgagg
     1321 cctggaccct gatggctgcg ctggcggcgg tcaccacccg ggtgcgtctc ggccagatgt
     1381 gtacctgtgc cctctaccgg cccccgtcgt tgctggccaa gatggcggcg aacatcgatg
     1441 tcatctcggg cgggcgtctc gacgtcggga tcggggccgg atggtcgaaa ggcgagttcg
     1501 aggcatatgg ataccgtttc ccctccgacc ggcagcgtct cgacatgctg gaggaggcgg
     1561 tccaggtgct gattgcgatg tggacccagg acgaggccgg cttccaggga gagcactact
     1621 cggtggatgg cgccatcaat cggccccacc cgatccagaa gcctcacccg ccgctttgga
     1681 tctccggtgg tggggaaaag cggaccctcc ggatcgtggc ccggtacggc gacttcgcca
     1741 acttcggcga caacctcgag gagttccggc acaagtcgcg ggtcctcgcc ggtcattgcg
     1801 aggcggtcgg cagaccctat gaggagatag gccgcaccat tcaccgtatg tcggtgattg
     1861 ggagggacga agccgacttg cgccgcaagc tggacatcgc cgcgggtcgc cgatcatgcg
     1921 caccggagga gttcgccgat gagcatttcg ccgccaccgt gccccaggcg gttgaggaga
     1981 tggggcggtt catcgacgag ggatgcgccg acatgatcct ctacttctac gacatggggg
     2041 aggccgacag cctcgagctg ttcgcctccg aggtgatccc tcaactggga tgagcgacct
     2101 ggatcgtctg ctcgatccgg ccaccatcgc catcgtcggt ctctccgccg aaccggcaaa
     2161 acatggaggc cgggtgctct ccaatctccg aatcctcgga ttcgagggcc aggtctgggg
     2221 agtcaacccc cggctcccgg aggtggacgg gatcgacgtg ttcgcgacgg tcgccga
//
LOCUS       DATITW010000457         1575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_703339, whole genome shotgun sequence.
ACCESSION   DATITW010000457 DATITW010000000
VERSION     DATITW010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703339"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1575)
                     /locus_tag="VLS86_06390"
     CDS             complement(<1..>1575)
                     /locus_tag="VLS86_06390"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HSO50158.1"
                     /translation="VDYSDLVTLSTHLLRRHPELARDIRDRYQTVVLDEYQDTNPAQR
                     ALLTTIFDDGFPVIAVGDEDQTIYEWRGASAENFELFAEHFRGRDGGPARERALTLNR
                     RSAPVILEIANEVRRHANPESEALASHDPDMPGQVVTHWATDALAEADWIARKFEALH
                     EQDIAWSEMAVLFRKNSDFAPVVEAMARHDIPVEVANLGGLLRVPEVSDLRAWLTVID
                     RPDDSPALVQILFGSRYRLGLADIAPLTRWLAGAEFDRDADDVPGVTLLEAIEHLTEI
                     DDLRPEARAVYAHFLDIYREVLTESQGMSLVEVCRLILDRTHAWQDIESLPANQRTTA
                     RLNLYRLLDLAEEWSPLRGRPSLAAFLEYLDAMADEPAEELDSAHLSGEEAVTLITVH
                     RSKGLEWEVVAIPAVTSQSFPGKARQFPDPLRFPEYLPVEHRIDRALDDMPTDEEQRT
                     AYFRARNELQEWRVAYVAATRARSQLIVTGAYWYGLPEPKMRPKQPSALFVLVEQLPS
                     TRQEGHAEESPRPALLR"
BASE COUNT          263 a          489 c          547 g          276 t
ORIGIN      
        1 cgtaacagag ccgggcgcgg cgactcttcg gcgtggcctt cctgacgcgt cgacgggagt
       61 tgttccacca gaacgaacaa ggccgacggc tgcttgggtc tcatcttcgg ctcgggcaac
      121 ccgtaccagt aggcgccggt gacgatgagc tgggaccggg ctcgggttgc ggccacgtag
      181 gcgacccgcc actcctggag ctcgtttcga gcacggaaat aggcagtccg ctgctcctca
      241 tcggttggca tgtcatccag ggcgcggtcg atgcgatgct cgacggggag atactcgggg
      301 aagcgcaacg gatccgggaa ctgacgggcc ttgcccggga agctctggct ggtgaccgcc
      361 gggattgcca ccacctccca ctccaggccc ttggaccggt gaaccgtgat gagggtgacg
      421 gcctcctccc cggaaagatg tgccgagtcc agctcctccg ccggctcgtc agccatcgca
      481 tccaggtact cgagaaaggc ggcaagcgat ggccggcctc ggagcgggct ccattcctct
      541 gccaggtcga gcagcctgta caggttgagt cgggcagtgg tgcgctggtt ggccgggagc
      601 gactcgatgt cctgccaggc gtgagtgcga tcgagaatca gacggcacac ctcgaccagg
      661 ctcattccct ggctctcggt caggacctcg cgatagatgt cgaggaagtg ggcgtagacc
      721 gctctcgcct cgggtcgaag atcgtcgatc tcggtgaggt gctcgatcgc ctcgagcaga
      781 gtcaccccgg ggacgtcgtc tgcatcgcgg tcgaattcgg cgccggcgag ccatctggtc
      841 agtggggcga tgtccgccag gcccagccgg tagcgacttc cgaacaggat ctgaaccagg
      901 gcgggggagt cgtcgggtcg gtcgatcacc gtcagccagg ccctgagatc ggacacctct
      961 ggaacccgga gcagtcctcc caggttcgcc acctcgaccg ggatgtcgtg gcgggccatg
     1021 gcttcgacca ccggggcgaa atcgctgttc ttccggaaga ggaccgccat ctcggaccag
     1081 gcgatgtctt gttcgtgcag cgcctcgaac ttgcgagcga tccagtcggc ctcggccagt
     1141 gcgtcggtgg cccagtgggt gaccacctga ccgggcatgt ccgggtcgtg agaggccagg
     1201 gcctccgact cggggttggc gtgacgtcgg acctcgttgg cgatttcgag gatcaccggg
     1261 gcggagcggc gattgagggt gagggcccgc tcgcgggcag ggcccccgtc ccgaccccgg
     1321 aagtgctcgg cgaacaactc gaagttctca gccgaggccc cccgccactc gtagatggtc
     1381 tggtcctcgt cgccgacagc gatcaccgga aacccgtcgt cgaagattgt ggtcagcaag
     1441 gccctctgtg caggattggt gtcctgatac tcgtcgagaa ccacggtctg ataccggtcg
     1501 cggatgtcgc gtgccagctc gggatgacgt cgaagcagat gggtggacag ggtgaccagg
     1561 tctgagtagt ccacg
//
LOCUS       DATITW010000458         1654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_743047, whole genome shotgun sequence.
ACCESSION   DATITW010000458 DATITW010000000
VERSION     DATITW010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743047"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1654
                     /locus_tag="VLS86_06395"
     CDS             <1..>1654
                     /locus_tag="VLS86_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSO50159.1"
                     /translation="KSLAVYEQGGVGMVMYNANDNQTLLTDNHWVPSVLVNNSDGLAL
                     KAYIDENGAEARVELTSAERERQRGRIMADFSSRGPVGSPASQDIIKPDVTAPGVQIL
                     AGNSLTPSAGPPDEPFQAISGTSMSSPHVAGLLALIKQAHPDWTPAMAKSALMTTARQ
                     DVREEDGQTRANPFDFGAGHVDPGGRAGAEGSLFNPGLVYRTRTIDYVGFLCDTSASY
                     LVAPLIGEDCDFLAGAGFQTQSENLNYPSIGVGEVPGSKTVIRTVTNVSDETASYRTD
                     VDEPRGFDVDVSPSQLRLEPGASASFEVTIVNEDAPIGEWRFGSLTWESGRTEVRSPI
                     AVKAAAIEVPRSVTGTGANGSASFDVAFGYNGPYAPAAHGMAADVPASGTVGFDEDQA
                     FDPTDVTANGATAHTFTLSGSAFLRIAMDVTDTTGNPDNDIDLYLYRGTELVAVSGAG
                     GIDELIELEAPADGGYTLYVHGWQVAEADPGVGYTFHFWDVKTAATSLAINPATPTTA
                     TLGTVGTVVVDWTGLAPPGSYLGAVSHSDGTTTFGLTLVEVDS"
BASE COUNT          307 a          547 c          545 g          255 t
ORIGIN      
        1 caagagtctc gccgtctatg agcagggcgg ggtggggatg gtgatgtaca acgccaacga
       61 caaccagaca cttctcaccg acaatcactg ggtgccaagc gtcctggtaa acaacagtga
      121 cggtctggcc ctgaaggcct acatcgacga gaatggagcc gaggcgcggg tcgagctgac
      181 ttctgcggag agggagcgcc agcggggcag gatcatggcc gacttctcct cccggggccc
      241 ggtcggcagc ccggcctctc aagacatcat caagcccgat gtgacggcgc caggggtgca
      301 gatcctcgcc ggcaacagcc tgaccccgag cgcgggcccg cccgatgagc cattccaggc
      361 catctccggg acctcgatgt cgagccctca tgtcgcagga ctgctcgccc tcatcaagca
      421 ggcacatccg gattggacac cggccatggc caaatcggcg ctgatgacca ccgcccgtca
      481 ggatgtccga gaggaagacg gccagaccag agccaatccg ttcgacttcg gggccggtca
      541 cgtcgacccg ggcggcaggg cgggcgccga aggctcactg ttcaacccgg gcctggtgta
      601 taggactcgg accatcgatt acgtgggatt cctctgtgac accagtgcca gctacctggt
      661 ggcgcccctc atcggagagg attgcgactt cctcgccggc gccggtttcc agacccagtc
      721 agagaacctc aactacccct cgatcggcgt cggcgaggtg ccggggtcga agaccgtgat
      781 ccggaccgtc accaatgtct cggacgagac tgccagctac cggaccgatg tcgatgagcc
      841 gcgtggtttc gatgtcgacg tgagcccgag ccaactcagg ctggaacccg gagcgtcggc
      901 cagcttcgag gtcacgatcg tcaatgaaga cgccccgatc ggagaatggc ggtttggctc
      961 gctcacctgg gagtcgggaa ggacagaggt tcgaagcccg atcgcggtca aggccgcggc
     1021 catcgaggtg ccccgatcgg tgaccggcac cggtgcgaac ggcagcgcca gcttcgacgt
     1081 cgcgtttggg tacaacggcc cctatgcgcc ggcggcgcac ggaatggcgg cggatgtccc
     1141 ggccagtggc acggtcggct tcgacgagga ccaggccttc gacccgaccg atgtcaccgc
     1201 caatggagcg acggcacaca cgttcaccct gtcgggatcg gcgttcctgc gaatcgcgat
     1261 ggacgtcacc gacaccacgg gcaacccgga caacgacatc gacctctact tgtaccgggg
     1321 cacggaactg gtcgcggtct cgggagccgg tggcatcgac gagctgatcg agctcgaggc
     1381 gccggccgac gggggctaca ccctgtacgt ccacggttgg caggtagccg aggccgaccc
     1441 cggtgttggc tacaccttcc acttctggga tgtgaagacg gcggcgacgt cgctcgcgat
     1501 caacccggcg acaccgacga ccgccaccct cgggacagtc ggcaccgtgg tggtcgactg
     1561 gaccgggctc gccccgccgg ggagctatct cggggccgtt tcccactcgg acggcacgac
     1621 caccttcggg ctcaccctgg tggaggtcga ttcc
//
LOCUS       DATITW010000459         1559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_102751, whole genome shotgun sequence.
ACCESSION   DATITW010000459 DATITW010000000
VERSION     DATITW010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102751"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VLS86_06400"
     CDS             <1..682
                     /locus_tag="VLS86_06400"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HSO50160.1"
                     /translation="RLVEAGYACAWLRYRTEVELGYHEGGPLVRDTRQGRELFNRSPL
                     EYEDEIAIIEHFKTLPYVDPDRIGLIGMSHGGEMVFKLTSEYHGARAAVAAEPAAHEF
                     LALTPDETAFVNEETRLRNIESMIMTDVDKVRARIDHDLSAARIAGIETPILVMGRES
                     DELQGIFRLSHELLLEAGKDVEWVTFDHPMHGYIYPVRGPGGGYQVDETQQAAIDYII
                     GYLDRHLG"
     gene            complement(740..>1559)
                     /locus_tag="VLS86_06405"
     CDS             complement(740..>1559)
                     /locus_tag="VLS86_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HSO50161.1"
                     /translation="AHAYRKACLHLYPPLQGVEIAHDGKMVTAHYRGPAQASPGHPVP
                     AVLVMCGADVFKEDRGWAGEMAFDNGMASLVMDGPGTGENPFPWAPESVSAWEAALDW
                     LAARPEVDGERVGAFGISRGGYSVMQLAGTVPGKLKAVVANAGHPFGYRMTESELEEF
                     IDHANRRATWAFGEPGGPPAFEPTSKEKEEAKFSRWALSEMGILDNITMPVLMINGKL
                     DHLAPIGNIYFMLEHGPATGKEARVYADAGHCAFKYFDDWAPASFRWLAEKLTR"
BASE COUNT          268 a          544 c          485 g          262 t
ORIGIN      
        1 ccgactggtc gaagccggtt acgcgtgtgc ctggctgagg tatcggaccg aggtcgagct
       61 cggctatcac gagggcggcc ccctggttcg cgacacgcgc cagggcagag agctgttcaa
      121 caggtcccct ctcgaatacg aggacgagat cgcgatcatc gagcacttca agacgctgcc
      181 ctacgtcgat cccgaccgca tcgggttgat cgggatgagt cacgggggcg agatggtctt
      241 caagctcacc tccgagtacc acggtgctcg agctgcggtc gccgccgagc ccgccgccca
      301 cgagttcctg gctctcactc ccgacgagac tgcgttcgtc aatgaggaga cccgactccg
      361 gaacatcgag tcgatgatca tgaccgacgt cgacaaggtg cgggcacgta tcgaccacga
      421 cctctccgcg gcgcgcatcg ccgggatcga gactccgatc ctggtcatgg gtcgcgagag
      481 cgacgagctc cagggcatct tccgtctcag ccacgagttg ttgctcgagg cgggcaagga
      541 cgtcgagtgg gtcaccttcg accatccgat gcacggctac atctaccccg tgcgtgggcc
      601 gggtggcggc taccaggtcg acgagaccca gcaggcagcc atcgactaca tcatcgggta
      661 cctcgaccgg catctcggct gaagccgagc gagccgagcc ctcgcccgtc tctccactca
      721 cccgaaatgg tgtccagggt catcgggtca gcttctcggc caaccagcgg aagctcgccg
      781 gcgcccagtc gtcgaagtac ttgaacgcgc agtgcccggc gtcggcatag accctggcct
      841 ccttcccggt cgccgggccg tgctccagca tgaagtagat gttgccgatc ggggccagat
      901 gatcgagctt gccgttgatc atcagcaccg gcatcgtgat gttgtcgagg atccccatct
      961 cggagagggc ccatctggag aacttcgcct cttccttctc cttcgaggtc ggctcgaacg
     1021 ccggtgggcc gccgggctcg ccaaacgccc aggtggcgcg gcggttggcg tggtcgatga
     1081 actcctcgag ctcggattcg gtcatgcgat acccgaatgg gtgcccggcg ttggccacca
     1141 ccgccttcag tttcccgggg accgtccccg ccagctgcat caccgagtag ccgccgcgcg
     1201 agatcccgaa ggcgcccacc cgctctccgt cgacctcggg tctggccgcc agccagtcga
     1261 gggccgcctc ccaggccgac accgactccg gcgcccaggg aaaggggttc tcccctgtcc
     1321 ccgggccgtc catgaccaac gaggccatcc cgttgtcaaa cgccatctcc cccgcccagc
     1381 cacgatcctc cttgaacaca tcggccccgc acatgaccaa caccgccggc accgggtgac
     1441 ccggggaagc ctgcgccggg ccccggtaat gggcggtcac catcttgccg tcgtgagcga
     1501 tctccactcc ctgcagcggg gggtagagat ggagacaggc tttgcgatag gcatgggca
//
LOCUS       DATITW010000460         1835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_222618, whole genome shotgun sequence.
ACCESSION   DATITW010000460 DATITW010000000
VERSION     DATITW010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222618"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..687
                     /locus_tag="VLS86_06410"
     CDS             190..687
                     /locus_tag="VLS86_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSO50162.1"
                     /translation="MARVLVLNASYEPLSVVTTRRAVVLVLHQKASVVVGKDEVWLSE
                     SASVEVPSVVRLNRYVKVPYRRAAPVTRRAVFGRDGHRCQYCGGPAESLDHIMPRSKG
                     GNHTWDNVVACCRPCNIRKGDRLPADAGLRLDSKPGPPRQYAWIYASTGYQVDPAWHP
                     YLGAA"
     gene            694..1257
                     /locus_tag="VLS86_06415"
     CDS             694..1257
                     /locus_tag="VLS86_06415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSO50163.1"
                     /translation="MTRIRLVSLDDATQIAAIYEPVVRRTAVSFELEPPTPDEIARRI
                     ESVSADHPWIVIEDEGALVGYAYATDFRQRPAYRWSTETTIYLDGDRLGQGLGKILYN
                     ALLEVAILWGYANAFAGIALPNPASEALHAAVGFQSLGVFPRAGFKMGRWHDVGWWHR
                     SLSLSESPPEPTRPSPESLERLLSSLT"
     gene            1458..>1835
                     /locus_tag="VLS86_06420"
     CDS             1458..>1835
                     /locus_tag="VLS86_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HSO50164.1"
                     /translation="MFLGEFFHSLDGKGRVVLPSTFRRDLEEGCVVAKGQDGQLVIFA
                     VDDFEAKAAEVMEKPQNRSGRRFSRTVFGGADHQDLDKSGRLLVKPDLRQFAALEGST
                     EIAVIGVYDHVELWNRDRYLSDRA"
BASE COUNT          319 a          577 c          617 g          322 t
ORIGIN      
        1 ctggaagtgg ttgcggtgga cgcccccatc gagcgaacgg gggaggccga gctcggcgag
       61 ggctggaccc aggcgctcgc gatcgcggtg ggggaggcca tcggtgccat ttccaagcct
      121 gacgaggtca ttgacacaaa cgtgcccagc cctgcttggt aacgtttcgg gcgcaacaaa
      181 gcgttcgata tggccagggt tcttgtcctc aatgcgagtt acgagccgct ctcggtggtg
      241 accacccgcc gggcagtcgt cttggtgctg catcaaaagg caagcgtggt ggtgggaaag
      301 gacgaggtct ggctctcgga gagcgcctcg gtcgaggtgc cctcggtggt tcggctgaac
      361 cgttacgtga aggtgccgta tcgccgggca gccccggtca cgagaagggc ggtcttcggg
      421 cgagatgggc accggtgtca atattgtggg ggcccggctg agtccctgga ccacatcatg
      481 cctcgctcaa aagggggcaa ccacacctgg gacaacgtcg tggcctgttg ccggccctgc
      541 aacatcagga agggcgaccg gctgccggcc gacgccgggc ttcgcctcga cagcaagccg
      601 ggaccaccac gtcagtacgc ctggatctac gcctcgaccg gctatcaggt cgatcccgcc
      661 tggcacccct atctcggggc tgcataagcc ggcatgaccc ggattcgcct cgtgtcgctg
      721 gacgacgcga cccagatcgc cgccatctac gagccggtgg tgcggcggac cgcggtgtcc
      781 ttcgagttgg agcctcccac tcccgatgag atcgcgcggc gaatcgaatc ggtgtccgcc
      841 gaccatcctt ggatcgtgat cgaagacgag ggcgccctcg tgggctacgc ctacgccacg
      901 gacttccggc agcggcccgc ataccgctgg tcgacggaga ccacgatcta cctcgacggc
      961 gaccgcctcg gccagggtct gggcaagatc ctctacaacg cattgctcga ggtggcgatc
     1021 ctgtgggggt acgccaacgc cttcgccggg atcgccctcc ccaacccggc cagtgaggct
     1081 ctccacgccg cggtcgggtt tcagtcgctc ggcgtcttcc cccgagcggg gttcaagatg
     1141 ggccggtggc acgatgtggg gtggtggcat cggtcactgt ccctgtccga gtcgcccccg
     1201 gagccgacga gaccgtcgcc ggagtccctc gagcgcctgc tctccagcct tacctgaggt
     1261 tggtggcgcc atatctggcg cctcgccaac agggaacctg ggaccaccac acgtccgcaa
     1321 gacgatcaca cccctgttgg gcacccaagt ggacccgagt gggggaaaag gttgacaagt
     1381 ggggggaagt gggtcatagt ggcgcttacg acccataagg gggtcatcca ggtgcggacg
     1441 gacggaaacg agaagaagtg ttccttggcg agttcttcca cagtctcgac gggaagggcc
     1501 gggtggtcct gccctccact ttccgtcgtg atctcgagga ggggtgtgtt gtcgccaaag
     1561 gtcaggacgg tcagctcgtc attttcgctg tcgacgactt cgaggcaaag gccgccgagg
     1621 tgatggagaa gccccagaat cggagtgggc gccgattctc gcggaccgtg ttcggcggcg
     1681 ccgatcatca ggatctcgac aagtcggggc gtctcctggt caaacccgat ctccgtcagt
     1741 tcgccgccct cgaaggcagc accgagatcg cagtcattgg tgtctacgac cacgtcgagc
     1801 tgtggaaccg agaccgctat ctcagcgacc gggcc
//
LOCUS       DATITW010000461         1553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_262706, whole genome shotgun sequence.
ACCESSION   DATITW010000461 DATITW010000000
VERSION     DATITW010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262706"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VLS86_06425"
     CDS             <1..741
                     /locus_tag="VLS86_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSO50165.1"
                     /translation="GFDGVWVFDHFTALYGDPGGPCLEAWTLLGALAAHTSRIRLGTL
                     VTGITHRHPSVLTTEIVTVDHVSGGRVECGLGAAWNENEHRSLGIPFPSTKERMQRLE
                     DAVEIFRLLTTGERVSYEGRHHSLDQAQYRPVPVQKPHPPIWIGANRPQLGLPLVGRQ
                     ADVWHGWGSDYAGKWDVVRRAAEKAGRDPDRITKSSSLSSSEPWDEVHKSFETHVANG
                     VSYLVVGWPSEGRKRLDEFIETVLPDLR"
     gene            complement(758..>1553)
                     /locus_tag="VLS86_06430"
     CDS             complement(758..>1553)
                     /locus_tag="VLS86_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HSO50166.1"
                     /translation="ERMTLAARALVYERESRRQSLIGFLADGYWQLLWERRRALGLST
                     LLLLGPGVAGLLWGMSDPVAATALVPDGFLWVTEAQDTNQGLGVVGLAGFSTFIMINN
                     IRVALLAFALGITFGLGTAYLVASNGLILGVVTGLALNAGNGDVMLAAIAAHGVLELS
                     CIVIAGAAGLSVGRALLRPAKKTRRAALTAEMSQAFRIAGGTAPWLVLAGLIEGYVSR
                     VGLGPVPTTIIGLIVGGVFWGLLVWRGRPINGQQTAVLAANPDSAV"
BASE COUNT          305 a          519 c          481 g          248 t
ORIGIN      
        1 ggtttcgacg gggtctgggt attcgaccac ttcaccgctc tctacggcga cccgggcgga
       61 ccatgtctcg aagcctggac tctcctcggg gcactggccg cccacacctc ccggattcgg
      121 ctcggcaccc tcgtcaccgg catcactcac cgtcatccgt cggttctcac gaccgagatc
      181 gtcaccgtcg accatgtatc gggcggcagg gtggagtgcg ggctcggcgc cgcctggaac
      241 gagaacgagc accggtccct cgggatccca tttccctcga ccaaggagcg gatgcagcgc
      301 ctcgaggacg cggtcgagat cttccgcctg ctgaccacgg gtgaacgggt gtcatacgaa
      361 ggccgtcatc actcgctcga ccaggcccag taccgcccgg ttcccgtgca gaagcctcat
      421 ccaccaatct ggatcggagc caaccgtccc caacttggac tgccgctggt cggtcgccag
      481 gccgacgtgt ggcacggttg ggggagcgat tacgcgggga agtgggatgt cgtgcgccgg
      541 gccgccgaga aggcgggccg tgaccccgat cggatcacca aatcatcgag tctttcgagc
      601 tcagaaccat gggacgaggt acacaagagt ttcgagactc acgttgccaa cggagtctcc
      661 tacctggtcg tcggctggcc atcggagggt cgcaagcgtc tcgacgagtt catcgagacc
      721 gtgcttcccg atctccgctg agtcaggtcg atgccaacta caccgccgag tccgggttag
      781 ctgccaacac cgcagtctgt tgaccattga tggggcggcc tcgccacacg aggagacccc
      841 aaaagacccc gccgacgatc aacccgatga tggtcgtcgg caccgggccc aacccgaccc
      901 ggctgacgta cccctcgatc aacccggcca gcaccaacca tggcgcggtt cccccggcga
      961 tgcggaacgc ctgactcatc tcggccgtga gagcagcccg ccgggtcttc ttggccggcc
     1021 tgagcagggc ccttcccaca ctgagcccgg cggctccggc gatcacgatg caagagagtt
     1081 ccagtactcc atgagcagca atcgcagcaa gcatcacatc gccattaccg gcgttgagag
     1141 ccagaccggt gacgactccg aggatcaagc cattactggc aacgaggtag gcggttccca
     1201 atccgaatgt gatcccgagg gcaaaggcga gcagcgcaac gcgaatgttg ttgatcatga
     1261 tgaaggtgga gaagccggcc aggccgacga ccccgagccc ttgattggtg tcctgggcct
     1321 cggtgaccca aaggaatccg tcgggtacga gggccgttgc cgccaccgga tcggacatac
     1381 cccacaagag gccggcgacg cccgggccga gcaagagcag ggtcgacaag ccgagagccc
     1441 gtcgccgttc ccaaagaagc tgccagtagc catcggccag aaaaccgatg agcgattggc
     1501 gccggctctc tcgttcatag accagggctc gggcggcgag agtcatgcgc tcg
//
LOCUS       DATITW010000462         1654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_463010, whole genome shotgun sequence.
ACCESSION   DATITW010000462 DATITW010000000
VERSION     DATITW010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463010"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VLS86_06435"
     CDS             <1..1063
                     /locus_tag="VLS86_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015938473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HSO50167.1"
                     /translation="LLENSVFLLIGLQVRWIVTDVADSTLGFGRIALACGAILITVMV
                     LRPLWVFPATYLPRKIRSVGRADPAPAWQYPAVVSWAGMRGVVTLAAAFVLPETVPHR
                     EILILAALVVTAGTLLIQGFTLPRLVRMLGLRGPDRREDTLQEAALLQEATKAGLAEL
                     DKLAAPDDPEDIVELLRTRTQERSRAAWERLGQAETETPSAAYSRLRQGMLLAERARV
                     LELRSTGRIPEEVLRNVLESFDIEESVLETLIDDDEDPKEDELMAPLSEHEGCDHLAA
                     APVSVAPLNPGGCEDCLREGTRWVHLRLCLTCGKVACCDSSPRRHAAMHYRVDGHPVV
                     RSIEPGEGWRWCYADSVLG"
     gene            complement(1039..>1654)
                     /locus_tag="VLS86_06440"
     CDS             complement(1039..>1654)
                     /locus_tag="VLS86_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50168.1"
                     /translation="GGTDLVGELEADAPGEGWTVERFEDGDIEGVEMTKEFSSLEELQ
                     ELLEEGRLSGPQEGLVGEVSFVDTGDTIRFEAEVPEGGDFEGIDPADMDGLFTYDARI
                     SITFPGEVIDHNGELEENTVTWTFDDPAAMAGSELFAEGRKGSGSAMVAIVGVVLGLG
                     VVALIVWQVLSRRKPPSDGLIAARLEHHTEPAPEAPAQPSTESA"
BASE COUNT          271 a          540 c          518 g          325 t
ORIGIN      
        1 tctgctggag aattctgtct tcctgctgat cggtctccag gtgcgttgga tcgtcaccga
       61 cgtggccgac agcactctcg ggttcggtcg catcgctctg gcctgcggag ccatcctgat
      121 cacggtgatg gtcctgcgcc cgttgtgggt gttcccggcg acttatctgc cgaggaagat
      181 ccgttcggtc ggacgggccg acccggctcc cgcctggcaa tacccggcgg tcgtttcctg
      241 ggccgggatg cgaggcgtcg tcaccctcgc cgcggcgttc gtccttcccg agactgtccc
      301 ccatcgggag atcctgatcc tggccgccct ggtagtgacc gccgggacct tgttgatcca
      361 gggtttcacc cttcccaggc tggtgcggat gcttggcctc cgggggcccg accgccggga
      421 agacacactt caggaagcgg cgttgcttca ggaagcaacc aaggccgggt tggccgaact
      481 cgacaagctc gcagcccccg acgatcccga ggacatcgtg gagcttctgc gaacccgtac
      541 ccaggagcga tcccgcgctg cttgggagcg gctgggccag gccgagacag agacaccctc
      601 cgccgcctac tctcggcttc ggcagggaat gcttctcgcg gagagagcgc gagtgttgga
      661 gctccgaagt acaggacgca tccccgagga agtgctccgt aacgttctcg aatcattcga
      721 catcgaagaa tccgtgttgg agactctcat tgatgatgac gaggacccca aggaagatga
      781 actgatggcc ccgctatccg agcacgaagg gtgcgaccac ctcgccgcgg cacctgtcag
      841 cgttgccccg ctcaatccgg gaggttgcga ggactgcctc cgcgaaggaa cccgctgggt
      901 ccacttgcgc ctgtgcctca cctgcggaaa ggtggcctgc tgcgattcat ccccgcgtcg
      961 tcacgccgcc atgcattatc gagtggatgg ccatcccgtc gttcgtagca ttgaacccgg
     1021 tgagggttgg cgctggtgct acgccgactc tgtgctcggt tgagccggag cctcgggagc
     1081 cggttcggtg tgatgctcca ggcgggcagc gatgagcccg tcggaggggg gcttgcgccg
     1141 ggagagaacc tgccacacga ttagcgcaac aacgccgagc ccgagcacga ctccgacgat
     1201 ggcaaccatc gccgagcccg accctttgcg accttcggcg aatagttcgc taccggccat
     1261 cgccgccgga tcgtcaaatg tccaggtcac ggtgttctct tcgagttcac cgttgtggtc
     1321 tatgacctcg cccgggaacg tgatgctgat gcgggcgtcg taggtgaaca gcccgtccat
     1381 atcggctgga tcgataccct cgaagtcgcc gccttcgggc acttctgctt cgaagcgaat
     1441 ggtgtcgccg gtgtcaacga acgaaacctc gccgacgagg ccttcttgcg gaccggagag
     1501 ccgtccctct tccaatagtt cttgcagttc ctcgagagag gagaactcct tggtcatttc
     1561 gacgccttcg atgtccccgt cctcgaatcg ctccaccgtc cagccttcgc ccggggcgtc
     1621 ggcctcgagt tcgccgacga ggtcggtccc gcct
//
LOCUS       DATITW010000463         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_743389, whole genome shotgun sequence.
ACCESSION   DATITW010000463 DATITW010000000
VERSION     DATITW010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743389"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VLS86_06445"
     CDS             complement(<1..636)
                     /locus_tag="VLS86_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSO50169.1"
                     /translation="MTKPGREMSMEYGEITYEVRGDAAWIGINRPEVRNAFREQTLDE
                     LAHALDSTRHDPTIVAAVVYGEGGHFSAGGDFHAMMKLNKANSYMWNDRMLRVAMTCR
                     NLPIPVIAMVEGACVGGGHELMLWCDLVIAAEDSTFGQTGARVGACPTVGATQYLSKL
                     IGERRAKEMIFLCKRYTGAEAAAIGLANEALPAPALRGRVEEIVQQIKGYSS"
     gene            complement(633..1625)
                     /locus_tag="VLS86_06450"
     CDS             complement(633..1625)
                     /locus_tag="VLS86_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSO50170.1"
                     /translation="MNDDKVRLASVGLGWWGSELAKAVSGTGRAEIVTCFARSEEGRE
                     RFAAEHGCRTAGSLEELLADPEVEGVVIATSNQSHRPLVEQAAAAGKHVFVEKPFTNT
                     VEDGVASVAAAREAGVLIQVGHQRRRTAAKRRIKAMLEAGELGDVETVVAHQSIPNGF
                     KMPDEAWRWDPEQSPLGSMTSLGVHKIDTMHYLVGPIRNVFAFTRPGRSHPIDEATIL
                     AMEFDSGALATLTTSFFTPVINEIAVFGTGGAAYSSGGGARLKVQGRDDPGPEEVDLE
                     PIDPVVDQMADFARAIRGEIPVEVDGEAGLAVIAVLEAAVLSVAERRPVEVARV"
     gene            1715..>2315
                     /locus_tag="VLS86_06455"
     CDS             1715..>2315
                     /locus_tag="VLS86_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSO50171.1"
                     /translation="MVTAAGERQADVAIEHGRVAAIGDIEPTGDVVDASGLHLLPGMV
                     DTHVHLMDPGETEREDFIAGTSAAAARGVTTIVEHTHSHPVRSVADLDEKRDYLKPRS
                     NVDFGLAAHVWPDRIGEIGPLAEHGVSFFKIFTCTTHGVPGLTTAEAADALTAIAQSG
                     ARCLIHNEDETLTRDAEKRLRALGRIDPGLLLEWRSREAE"
BASE COUNT          391 a          812 c          728 g          384 t
ORIGIN      
        1 cgatgagtag cccttgatct gctggacgat ttcctcgacc cggccgcgga gggcgggagc
       61 cgggagcgct tcgttggcga ggccgatcgc ggcggcctcg gctcccgtgt accgcttgca
      121 gagaaagatc atctccttgg cccggcgctc gccgatgagc ttggagaggt actgggtggc
      181 tccgacggtg ggacaggccc ccacccgcgc cccggtctgg ccaaaggtgg agtcctcggc
      241 ggcgatgacg agatcgcacc acaacatcaa ctcgtggccg ccgccgacac acgccccttc
      301 caccatggcg atgaccggga tcggcaggtt gcgacaggtc atcgcgaccc gaagcatgcg
      361 gtcgttccac atgtaggagt tggccttgtt cagcttcatc attgcgtgga agtcgccacc
      421 cgccgagaag tgaccgcctt ctccatagac caccgcggcg acgatcgtcg ggtcgtgacg
      481 cgtcgagtcg agggcatgag ccaactcgtc gagggtctgc tccctgaagg cattgcgcac
      541 ctcgggtcgg ttgatcccga tccaggcggc gtcgccccgc acctcatatg tgatctcacc
      601 gtattccatc gacatctccc ttcccggctt ggtcaaaccc tggcaacctc gaccgggcgc
      661 cgctcggcga ccgagaggac ggcggcttcg agcacggcga tcaccgcaag accggcctca
      721 ccgtcgacct cgaccgggat ctcgccgcga atcgcccgag cgaagtccgc catttgatcg
      781 accaccgggt cgatcggctc gaggtccacc tcctcgggcc ccgggtcgtc acgtccctgg
      841 accttcaggc gagcaccacc tccactgctg tatgcggctc ccccggttcc gaagacggct
      901 atctcgttga tcaccggcgt gaagaacgag gtggtgagcg tggccagcgc cccgctgtcg
      961 aactccatgg cgaggatcgt ggcctcgtcg atcggatggg agcgcccggg tcgggtgaag
     1021 gcaaacacat tgcgaatcgg cccgaccaga taatgcatgg tgtcgatctt gtgcacgccg
     1081 agcgaggtca tcgagcccag cgggctctgc tcgggatccc agcgccaggc ctcatcgggc
     1141 atcttgaagc cattcgggat cgactgatgg gcgaccaccg tttccacgtc ccccaactcc
     1201 cccgcctcga gcatcgcctt gatccggcgc ttggccgcgg tccgccgtcg ctgatgaccg
     1261 acctggatga ggacgcccgc ctccctggcg gcggcaaccg aagccacccc atcctcgacc
     1321 gtgttggtaa atggcttctc gacgaagacg tgcttgccgg cggcggcggc ctgctcaacc
     1381 agggggcgat gactctggtt cgaggtggca atgaccaccc cctccacttc ggggtcggcg
     1441 aggagctcct cgagtgatcc cgcggtccgg cacccgtgct cggcggcgaa gcgttcgcgg
     1501 ccctcctcgc tcctcgcgaa gcaggtcacg atctcggcgc gcccggtgcc ggacaccgct
     1561 ttggccagct ccgagcccca ccacccgagg ccaacgctgg cgagccgcac cttgtcgtcg
     1621 ttcatatatc cccttggtcc aaaaggcgat cctatatgtt gcgtgatatc gatcggaggt
     1681 ttgggtcacg atcatcacga tcaagggagg gacggtggtc accgccgccg gtgagcggca
     1741 ggcagatgtg gcgatcgagc acggaagggt ggccgcaatt ggcgacatcg agccgaccgg
     1801 agatgtcgtc gacgccagcg gcttgcacct cctccccggg atggtggaca cccacgtcca
     1861 tctcatggac ccgggggaga ccgaacgcga ggacttcatc gccggcacca gcgccgccgc
     1921 cgcccgcggg gtgaccacca tcgtcgagca cacccactcc catccagtcc gatcggtggc
     1981 cgacctcgac gagaagcgcg actacttgaa gccgcgatcc aacgtcgact tcggtctggc
     2041 cgcccacgtc tggccggatc ggatcggcga gatcggccca ctggccgagc atggggtcag
     2101 cttcttcaag atcttcacct gcaccaccca cggcgtcccc ggcctcacca cggccgaggc
     2161 ggccgatgcc ctgaccgcca tcgcccaatc cggtgcccgc tgcctgattc acaacgagga
     2221 cgaaaccctc acccgcgacg ccgagaagcg tctcagggcg ctgggcagga tcgaccccgg
     2281 ccttcttctc gaatggcgga gccgggaggc ggagc
//
LOCUS       DATITW010000464         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_22929, whole genome shotgun sequence.
ACCESSION   DATITW010000464 DATITW010000000
VERSION     DATITW010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22929"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VLS86_06460"
     CDS             complement(<1..698)
                     /locus_tag="VLS86_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSO50172.1"
                     /translation="MTDAVVQGILLGGLYALFAAGLAIVFGVMKIVNLAHGDFIILSA
                     YLTLVVVEVTSLNPMVTVALIAAIMGLFGYALQRGILNFTLGSGILPPLVVTFGLSII
                     IQNLLLEVFSADTRGLDAGALELSSMQLPFGIAVGWFPVLTFLLSVAVLGGLQLVFGH
                     TRMGRSFRAVSDDEETARLMGVRTRHVFGMTMAIALVVTAIGGVVLGIRTQFDPALGP
                     ARLIYAFETVIIGGL"
     gene            complement(695..1405)
                     /locus_tag="VLS86_06465"
     CDS             complement(695..1405)
                     /locus_tag="VLS86_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50173.1"
                     /translation="MPDILEVRALDAFYGPFQALFATSLAVAEGEIVAVIGANGAGKS
                     TLLRSISGWLGSVPDQILFEGEPIGGERAERVARRGVVMVPEGRRIFPTLSVEENLMM
                     GGYTDRPGTWDLGRVYELFPVLRERRSALGTTLSGGQQQMLAIGRGLMANPRILLLDE
                     ISLGLAPIVVKELYRVIPTLREDGISVLLVEQDVRQATTTADRIYCLLRGRVSLEAQA
                     NEVDHADLVSAYFGVRDA"
     gene            complement(1413..2135)
                     /locus_tag="VLS86_06470"
     CDS             complement(1413..2135)
                     /locus_tag="VLS86_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018448887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50174.1"
                     /translation="MGLALEITGVSKSFGALKVIDDLSVTLGEGEALGIVGPNGAGKT
                     TLFNLITGSIPLDSGTIRFDQSAIVAQAPYLRVRRGLGRTYQIPRPFGGMTVFENVLV
                     AASYGSSQGETGGHERCVEILASTGLLAKANTLAGTLTILERKRLELARALGTGPTVL
                     LLDEIAGGLTDPEVEELVVLIKSIHNSGMSIVWIEHLLHALVAVVDRVQAIHFGRHLI
                     EGNPEEIINSPEMREVYLGIEE"
     gene            complement(2151..>2381)
                     /locus_tag="VLS86_06475"
     CDS             complement(2151..>2381)
                     /locus_tag="VLS86_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSO50175.1"
                     /translation="EDKQSILDAVAGTSMETLVGPVDWTTPGPVPNVTTTPLVGGQWV
                     LGSGDFKYDLLVVDNSQTPSVPLTGEIKPIGG"
BASE COUNT          487 a          791 c          702 g          401 t
ORIGIN      
        1 agcccgccaa tgatcaccgt ctcgaacgcg tagatcaggc gagctgggcc aagtgcgggg
       61 tcgaactggg tccggatccc gaggaccact ccaccaatcg ctgtcaccac cagcgcgatt
      121 gccatggtca tgccgaacac atggcgggtt ctgaccccca tgagacgcgc ggtttcctcg
      181 tcgtctgaaa ccgcccggaa ggaccggccc atccgggtgt gaccgaagac cagttggagc
      241 ccgccgagca ctgccaccga aagcagaaag gtcaagacgg ggaaccatcc gacggcgatt
      301 ccaaacggga gctgcatgct gctcaactcg agcgccccgg cgtcgagccc ccgagtatcg
      361 gccgagaaca cctccagcag cagattctgg atgatgatcg aaagcccgaa agtgaccacc
      421 aagggcggaa gaatcccgga gccgagagtg aagttgagga tcccccgctg caacgcatac
      481 ccgaacaacc ccatgatcgc cgcgatcagc gccacggtca ccatcgggtt cagggatgtc
      541 acttccacga ctaccagggt cagatacgca gagaggatga tgaagtcacc gtgagccaga
      601 ttcacgatct tcatcacccc gaagacgatc gcgagcccgg ctgcgaacag ggcgtagagc
      661 ccgccgagga ggatcccttg gacgacggcg tcggtcatgc gtcccgcacg ccgaagtagg
      721 ccgagaccag gtccgcatga tcgacctcgt tggcctgggc ctccaaagag acccgaccac
      781 ggagcaagca gtagatccga tcggcagtcg tcgtcgcttg ccgaacatcc tgctcgacca
      841 gcaagaccga aatcccgtcc tcgcggaggg ttggaataac ccgatacagc tccttcacca
      901 cgatcggggc caggccgagc gagatctcat cgaggagcag gatccgggga ttggccatca
      961 gcccgcggcc gatcgccagc atctgttgct ggccgccgga gagtgtggtt cccagcgccg
     1021 aacggcgctc ccgaagcacc gggaacagct cgtagacgcg gcccagatcc caggtccctg
     1081 gtcggtcggt gtagcccccc atcatcaggt tctcctcgac actgagggtc gggaagatcc
     1141 tcctgccctc cggcaccatg accacaccgc gccgggcaac cctttcggcc cgttcgccgc
     1201 caatcggctc accctcgaac aggatttgat ccggcaccga tcccagccag cccgagatcg
     1261 acctgaggag tgtggacttg ccggcgccat tggccccgat caccgcaact atctctcctt
     1321 cggcgaccgc caaggacgtc gcgaagaggg cctgaaatgg cccatagaaa gcatcgaggg
     1381 cccttacctc gaggatgtcg ggcatcgtct actcactctt cgatcccgag gtatacctcg
     1441 cgcatctcag ggctgttgat tatctcctcg gggttgccct cgatgagatg cctgccgaaa
     1501 tgaatggcct ggactcgatc caccaccgca accagcgcat ggagcaggtg ctcgatccag
     1561 acaatcgaca tgcccgagtt gtgaatcgac ttgatcagga cgacaagctc ttccacttcg
     1621 ggatctgtca gcccgccggc aatctcatct aatagaagca ccgtcgggcc ggttcccaac
     1681 gccctcgcca gttcgagtcg ctttcgctcc aggatcgtca aggtgccggc gagcgtgttg
     1741 gccttcgcca ggagcccggt gctggccagg atctccacac atcgctcgtg acctccggtc
     1801 tcgccctggg acgatccata ggaagcggcg accagaacat tctcgaagac ggtcatgcct
     1861 ccgaacggcc gggggatttg ataggtccgg cccaggccgc gacgcacccg gagatagggc
     1921 gcctgagcaa caatcgccga ctgatcaaac cggatcgtcc cactgtcgag tgggatcgat
     1981 ccggtgatca aattgaacag cgtcgtcttg cccgcgccgt tggggccaac gatcccgagg
     2041 gcttctcctt cgcccaacgt gacactcagg tcgtcgatga ccttgagagc cccaaaggac
     2101 ttgctgacgc ctgttatctc gagagcgagc cccacctcgg cagtcgcccg ctagccaccg
     2161 attggcttga tctcaccggt gagtggcacc gatggggtct gcgagttgtc cacgaccagg
     2221 aggtcgtact tgaagtcgcc gctgcccagc acccattgtc cgcccaccag gggtgtggtg
     2281 gtgacgttgg gcacagggcc gggcgtcgtc caatcgacgg gcccgaccag cgtctccatg
     2341 ctggttccgg cgacggcatc gaggatcgac tgcttgtcct c
//
LOCUS       DATITW010000465         1613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_302974, whole genome shotgun sequence.
ACCESSION   DATITW010000465 DATITW010000000
VERSION     DATITW010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302974"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VLS86_06480"
     CDS             <1..314
                     /locus_tag="VLS86_06480"
                     /inference="COORDINATES: protein motif:HMM:NF036800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage 0 sporulation protein J"
                     /protein_id="HSO50176.1"
                     /translation="GHARALVGLEDEAYATHLAQRAVDDGWSVRQIEDAVRARKAADR
                     REPTGVRQLRPVEIIELEKRLTDRLGAAVKINYRNEKGKVEIRFASLAELERLYRMFS
                     G"
     gene            complement(304..1272)
                     /locus_tag="VLS86_06485"
     CDS             complement(304..1272)
                     /locus_tag="VLS86_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HSO50177.1"
                     /translation="MRLYRAGDEGAAVRDIQDRLDALGFTCDPDPRSTFGDGTRDAVR
                     GFQKARGLDVDGIVGPDTWRSLYEAGYRLGDRMMFLRRPMMRGEDVSELQSRLSSLGF
                     DPGKVDGVFGPRTEAAVVEFQHNRDLAEDGKVGPEVVTEIHLVTRGEMKEGRQGVRER
                     EWLRRLPPTVAGARVYLDAGCRDAHEATVAWDAASATALGLQEQGGLPVMSRSQDASL
                     PERLRARRANVLGSDLIVAFQVNADGEDFVFYFASEHGSSAAGELLAKSIAESAGGRV
                     EGRASAMLKETRAPAVVVSRAKLDEEVGYAVADGLTRFFANAAATR"
     gene            complement(1370..>1613)
                     /gene="trxA"
                     /locus_tag="VLS86_06490"
     CDS             complement(1370..>1613)
                     /gene="trxA"
                     /locus_tag="VLS86_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSO50178.1"
                     /translation="FWAEWCGPCRVIAPVLEEIAAEHESLTIGKLNIDENPMPAAKHD
                     VMSIPTLILFKDGVEKKRIVGAAPKHRLLQELSEFL"
BASE COUNT          268 a          534 c          544 g          267 t
ORIGIN      
        1 cgggccacgc ccgcgccctg gtcggtctcg aggacgaggc ctacgccacc catctcgccc
       61 agcgtgcggt ggacgacggt tggagtgtgc gtcagatcga ggatgcggta cgggctcgca
      121 aggccgctga ccgtcgagag ccgacagggg tgcgccagct ccggccggtg gagatcatcg
      181 aactggagaa gcggctcacc gaccgtctcg gggccgcggt caagatcaac tatcgcaacg
      241 agaaggggaa ggtggagatc agattcgcct ctctggcgga gctggagcgt ctctaccgga
      301 tgttcagcgg gtagccgccg cgttggcgaa gaaccgggtg agaccgtcgg cgactgcgta
      361 gccgacctct tcgtcgagct tggccctgga aaccaccact gcgggcgccc tggtctcttt
      421 gagcattgcg ctggcccgtc cctcgacccg gcccccggcg ctctcggcga tcgacttggc
      481 cagcagctcc ccggcggccg aggagccgtg ctcagaggcg aagtagaaca cgaaatcctc
      541 gccatcggca ttgacctgga aggccacgat caggtcggag ccgagcacgt tggcccgtcg
      601 ggcgcggaga cgctcgggga gcgaggcgtc ctgagatctc gacatcaccg ggagaccgcc
      661 ctgctcctga agaccaaggg cggtggcaga ggccgcgtcc caggcgaccg tggcctcgtg
      721 ggcatcgcga catccggcgt cgagatagac cctggcgccg gccacggttg ggggaagtct
      781 tcgcagccac tcccgctccc ggaccccctg tcgaccctct ttcatctctc cccgggtcac
      841 gagatggatc tcggtcacca cctcggggcc tacttttcca tcttctgcca ggtcgcgatt
      901 gtgctggaac tcgaccacgg ccgcttcggt tcggggtccg aacacgccgt cgaccttgcc
      961 gggatcgaag ccgagtgagc tgagccggga ctggagctcg gacacgtctt cgccccgcat
     1021 catcggtcgt cgcaggaaca tcatccggtc accgagtcgg tagccggcct cgtagaggga
     1081 acgccaggtg tcgggcccga cgatgccatc gacgtcgagg ccccgagcct tctgaaagcc
     1141 ccggaccgcg tcgcgggtgc cgtcgccaaa agtggagcgt ggatcgggat cgcaggtgaa
     1201 tccgagggcg tcgagacggt cctgtatgtc gcgaaccgcc gccccctcgt cacccgcgcg
     1261 atagaggcgc atggggctct acgttatccg tttctcgcca catggttccg ggggccgcca
     1321 cgctccgtgg accggctgcg gcccaggccc cgggatcgac cggggaggat cagaggaact
     1381 cggagagctc ttggaggaga cgatgcttgg gagcggcacc gacgatccgc ttcttctcga
     1441 caccgtcttt gaacaggatc agggtcggga tgctcatcac gtcgtgcttg gcggccggca
     1501 tcgggttctc gtcgatgttg agcttgccaa tcgtcagcga ctcgtgctcg gcggcgatct
     1561 cctcgaggac gggcgcgatc acccgacatg gcccacacca ctccgcccag aaa
//
LOCUS       DATITW010000466         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_103153, whole genome shotgun sequence.
ACCESSION   DATITW010000466 DATITW010000000
VERSION     DATITW010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103153"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /locus_tag="VLS86_06495"
     CDS             <1..1598
                     /locus_tag="VLS86_06495"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSO50179.1"
                     /translation="QLRRLVSGRGIGDRDLAGGERIMLPALILLPVAAALIVALVPSR
                     RREIHLPLGVALSVAPLALAGYLFWVFEPAAEYQFVERAVWYEPWGISWYLGVDGISL
                     PMVLLTALLVPIALGASTAIKVRTKEFVVYTLLLEAGMIGSFLALDLFIFFVFFEAIL
                     IPMYFIIGIWGGERRVYAAVKFFIYTAFGSALMLAGIIALAFNHSSQFGAPSFALEEL
                     MNLDLSPGAETLLFAAFALAFGIKVPLFPLHTWLPDAHVEAPTAGSVLLAGVLLKLGT
                     YGLLRFNLSLFPETSVDAVQIMGVVAVIGIIYGAVVAIVQPDLKKLVAYSSVSHLGFI
                     VLGTFALTSGGLQGAVIQNVNQGLTTGALFLLVGMIYERRHTKQISDFGGLQKVMPLF
                     AGFFLFMAFASIGLPGLNGFVGEFLVLIGSFTTLPVLTVVAAFGVVLAAIYLLWAYER
                     VFTGVPDNEANLSLKDLSVRELSLLAPLAILVLILGLYPAVLLDKITPSTEAVLDHIE
                     ATTSYVTPVPGRISDVVVVEAAE"
     gene            1595..>2225
                     /locus_tag="VLS86_06500"
     CDS             1595..>2225
                     /locus_tag="VLS86_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSO50180.1"
                     /translation="MIVTPEVSWIAIAPDLVLALGAAAVLLVEVQWKPPARVLGYVTA
                     ATLVAAIGLSVLQWTLAGDAVAAGDQDTLLAFSNMVTMDGLAVFARLALLLATGLGLL
                     AGWRFVQGLGRRGAEGLALVLLATTGFSIMVASNNLVMMFLGLEVGSIALYILAGMTR
                     ERAESDEAAIKYFLLGSFASALFIYGVALIYAGTGFFAILDIRQYFSEFV"
BASE COUNT          296 a          707 c          699 g          523 t
ORIGIN      
        1 cgcaactacg gcgccttgtt tctggccggg gcattggtga tcgtgatctg gctggtggtg
       61 aacggataat gctgcccgca ttgatcctgc tcccggtggc ggcggcattg atcgtggccc
      121 tggtcccctc ccgacgccgg gagatccatc tcccgctcgg cgtcgcgctc tccgttgccc
      181 cgttggcact ggccgggtac ttgttctggg tgttcgagcc ggcagccgag tatcagttcg
      241 tggagcgggc ggtctggtac gagccctggg gcatcagctg gtatctgggg gtggatggca
      301 tctcgctccc catggtcctg ctcaccgcat tgctggtccc gatcgctctc ggggcatcga
      361 ccgcgatcaa ggtgcgcacc aaggaattcg tggtctacac gctgctcctg gaggcgggga
      421 tgatcgggtc gttcctggct ctcgacctgt ttatcttctt cgtcttcttc gaggcgatcc
      481 tcatcccgat gtacttcatc atcgggatct ggggcggtga gcgcagggtc tacgccgcgg
      541 tcaagttctt catctacacc gcttttggat cggcgctgat gctcgccggc atcatcgccc
      601 tcgccttcaa ccactcctca cagttcgggg ctccttcgtt tgctctcgag gaactgatga
      661 acctcgacct gagcccgggg gcggagaccc tgctttttgc agcctttgcc ctggcctttg
      721 ggatcaaggt cccgctgttc cctctgcaca cctggctccc cgacgcccat gtcgaggccc
      781 cgaccgcggg ctcggtcctg ttggcgggcg tgctcctgaa gttgggcacc tatggcttgc
      841 ttcgattcaa tctcagcctg ttccctgaga cttcggtcga cgcggtgcag atcatggggg
      901 tcgttgcggt gattgggatc atctacgggg cggtagtcgc catcgtccag cccgatctca
      961 agaaactggt ggcttactcg tcggtcagcc atctcgggtt catcgtcctg gggaccttcg
     1021 ccctgacttc gggtggtctt cagggggcgg tgatccagaa cgtcaaccaa ggcctgacca
     1081 cgggagccct gttcctcctg gtggggatga tctacgaacg ccgccacacc aagcagatct
     1141 cggacttcgg cgggttgcag aaggtgatgc cgctctttgc cggcttcttc ttgttcatgg
     1201 cgtttgcctc gattggcctg cccggcctca acgggttcgt gggggagttt ctggtcctga
     1261 ttgggagctt tacgacgctc cccgtcttga ccgtggtggc ggcgtttggt gtggtgctgg
     1321 ccgccatcta tctcctgtgg gcctacgagc gggtgttcac cggcgtgccg gacaacgagg
     1381 ccaacctcag cttgaaggat ctttcggtga gggaactgtc tctgctcgcc ccgctcgcga
     1441 ttctggtgct catcctcggg ctctatcccg cggtcctgct cgacaagatc acgccgtcga
     1501 ccgaggcggt cctcgaccac atcgaagcca cgacgagcta cgtcacccct gttccgggac
     1561 ggattagtga cgtggtcgtg gtggaggcgg ccgaatgatc gttacccccg aggtctcttg
     1621 gattgcgatt gctcccgacc tggtgcttgc cctcggagcg gccgcggtgc tcctcgtcga
     1681 ggtccaatgg aagccaccgg cccgcgtcct gggatatgtg accgcagcca ccctggtggc
     1741 cgccatcggg ctttcggttc tgcagtggac cctggccggc gacgcggtcg cagccgggga
     1801 tcaggacacg ctccttgcct tctccaacat ggtgaccatg gacggtctcg cggtcttcgc
     1861 cagactggcg ctcctcctcg cgaccgggct cggcctgctt gccggatggc gctttgtcca
     1921 aggtctcggc cggcgaggcg ccgagggcct ggcgttggtg ctgctggcca ccaccggctt
     1981 ttcgataatg gtcgcctcca acaacctggt gatgatgttc ctcggtctcg aggtgggctc
     2041 gattgccctc tacatcctgg ccgggatgac ccgggagcgg gcagagtccg acgaggccgc
     2101 gatcaagtac ttcctcctcg ggtcgttcgc ctcggccttg ttcatctacg gagtggctct
     2161 catctacgcc gggaccgggt tcttcgccat cctcgacatt cgccaatact tctccgagtt
     2221 cgtgg
//
LOCUS       DATITW010000467         1751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_703879, whole genome shotgun sequence.
ACCESSION   DATITW010000467 DATITW010000000
VERSION     DATITW010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703879"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VLS86_06505"
     CDS             complement(<1..475)
                     /locus_tag="VLS86_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50181.1"
                     /translation="MTASFPSRLRAALVIAAISGSLLWIAGTAVASDTSTRWESFSEA
                     TACGEPYTRTPTMSKTGVISSTEPILGPFGTYFGRSISQVRTELVYWTVPGSGGVRVP
                     VHRAALPAFQQVAAGLAAQAAAGRVYSVTRVSSFFGRTLSGSHQLSRHALGTAIDI"
     gene            complement(472..1431)
                     /locus_tag="VLS86_06510"
     CDS             complement(472..1431)
                     /locus_tag="VLS86_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020405461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HSO50182.1"
                     /translation="MATPPTSTTTPEAAGPPAGSELELVSFPVPAGSGPHDVAPAVDG
                     GVWYTAQASGELGWLDPVSGQTRHTGLGPGSRPHGVIVDAVGVPWVTDSGLNAIVSVD
                     PATEEVTVHPLPDDRADSNLNTASFDGDGVLWFTGQSGIYGQLDPATGHLEVFDAPMG
                     RGPYGITTTPGGAVYYASLAGSFVGAIGEDGTTSVIEPPTPDRGARRVWSDSAGQIWV
                     SEWNSGNLSRYTPETDSWQTWPLPGDNPQTYAVYVDEHDLVWASDFGSNSMVRFDPDT
                     EQFDVFPLPHDPGEVRQILGRPGEVWAPESSADNLVLIRERGS"
BASE COUNT          291 a          576 c          624 g          260 t
ORIGIN      
        1 tgatgtcgat cgccgtgccc agggcgtgcc gtgacagctg gtgtgacccg ctcagggttc
       61 gaccgaagaa agagctcacc ctggtcaccg agtacacccg gccggcagca gcctgggcgg
      121 caagcccggc cgcgacctgc tgaaaggccg gcaaagcagc ccgatgcacc gggaccctga
      181 ccccgccgct gcccggcacc gtccaataca cgagctcggt tctcacctgg ctgatcgagc
      241 gcccgaagta ggtcccgaac ggcccgagaa tcggctcggt gctcgagatg acaccggtct
      301 tggacatggt cggggtccgg gtgtagggct ccccacaggc ggtggcctcg gagaagctct
      361 cccagcgggt cgaggtgtcc gaggcgactg ccgttcccgc gatccagagg agggaaccgg
      421 agatggccgc gatgacgagg gccgctcgaa gccggctggg gaaactggcg gtcatgaccc
      481 tcgttcccgg atcaacacga gattgtcggc ggacgactcc ggggcccaca cctcgccggg
      541 ccgccccagg atctggcgta cctccccggg gtcgtgaggc agggggaaca catcgaactg
      601 ctcggtgtcg ggatcgaatc gaaccatcga gttcgacccg aaatcgctgg cccacaccag
      661 atcgtgctcg tcgacgtaga ccgcgtaggt ctgcgggttg tccccgggga gcggccaggt
      721 ctgccagctg tcggtctcgg gcgtgtatct gctcagattc cccgagttcc actcgctgac
      781 ccagatctgg cccgccgagt ccgaccagac ccgacgggca ccccgatcgg gagtgggggg
      841 ctcgatcacc gatgtcgtcc cgtcctcccc gatggccccg acgaacgaac cagcgagcga
      901 ggcgtagtac acagctccac ccggcgtggt ggtgattccg tagggccctc tgcccatcgg
      961 ggcgtcgaac acctcgaggt gaccggtggc cgggtcgagc tgcccgtaga tcccgctctg
     1021 accggtgaac cagagcaccc cgtcgccgtc gaagctcgcg gtgttgagat tggaatcggc
     1081 tcgatcgtcg gggagggggt ggaccgtgac ctcctcggtc gccggatcga cactgacgat
     1141 ggcgttgaga ccgctgtccg tgacccaggg gactccgacc gcgtcgacga tgactccgtg
     1201 tggcctggag cccgggccga gaccggtgtg ccgggtctgt cccgagaccg ggtcgagcca
     1261 acccagctca cccgaggcct gcgccgtgta ccagacaccg ccgtcgacag ccggggccac
     1321 atcatggggc ccggacccag ccgggacagg gaaggagacc agctccaact cgctgccggc
     1381 aggtgggccc gctgcttcgg gcgtggtggt ggaagtgggt ggagtcgcca cggtgctgct
     1441 ggccgatcct ccggtgtcgg tggtcgaagt ggatgagggc gaagtggtgg atacggggtc
     1501 gcgctggtgt ccccggtggt ggcggtgcag gcggcggccg acacgacaat gagcgccaga
     1561 agcgaacgca gcatcgagag cagcctaagc aggccgatcg cttaggtagc aagagggctc
     1621 ggtgcggagg gtcagccgtc ctcggccgcc acctcccgga ggcgacgcac ggcgtcactc
     1681 actcccccgc tccacggtgg cccgtggccg ggaaggaccc aggtggcggt cacgtcctgg
     1741 agcctggaga g
//
LOCUS       DATITW010000468         1725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_543837, whole genome shotgun sequence.
ACCESSION   DATITW010000468 DATITW010000000
VERSION     DATITW010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543837"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VLS86_06515"
     CDS             complement(<1..1123)
                     /locus_tag="VLS86_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HSO50183.1"
                     /translation="MPETAQLTLPVLPLHNGVVFPHMVVTLRIETEEGKTALAAVAKS
                     NGRLRLVPRLEGRYAAVGTIAEIQQSDDNGNVVVSGVARARVGAGSVDETGALWVTAE
                     PYPEVDMLDDEFDALVAEYRAVVSAVLENRGMAGVAERLLAVDNPSQLADFAVYSPDL
                     NLEQKVELLEALDIRQRLGMLLVWMREVLADLELRRKVRDDAAERIEKSQKDYILRQQ
                     LDAIRKELGEEGDIASEYRAKLAERDLPEKVHEAIEREIDRLERMSDQSPESSWVRTW
                     LDTMFELPWGTTTEDRLDLDEAWTVLDADHTGLSDVKERIIEHLAVRKLRAERGLDDE
                     AARNGGAILLLVGPPGVGKTSLGESVARALGRKFVRVALG"
     gene            complement(1242..1628)
                     /locus_tag="VLS86_06520"
     CDS             complement(1242..1628)
                     /locus_tag="VLS86_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50184.1"
                     /translation="MTTASIIAAVLLGVVSVFQIALALGLPAGRAAWGGRHEGKLPPG
                     LRVASGIAGVFLYPIIILLVLTAGGAIDARDPIPKLGATGMWILTGLFTVGTIANLAS
                     RSKPERWWGLVSATIAICCGIVAGSL"
BASE COUNT          281 a          595 c          545 g          304 t
ORIGIN      
        1 ctcccagtgc caccctgacg aacttgcggc cgagcgcccg ggccaccgac tcgcccaggg
       61 aggtctttcc caccccgggc gggccgacca gcaacaggat cgcaccaccg tttcgggcgg
      121 cctcgtcgtc cagcccacgc tcggcgcgga gcttgcgcac tgcgagatgc tcgatgatcc
      181 gctccttgac gtcgctcaac ccggtgtggt ccgcgtcgag cacggtccac gcctcgtcga
      241 gatcgagccg gtcctcggtg gtggtgcccc agggaagctc gaacatggtg tcgagccagg
      301 tgcggaccca ggagctctcc ggggattgat cgctcattcg ctccaggcgg tcgatctcgc
      361 gttcgatcgc ttcgtgcacc ttctcgggca gatcccgctc ggcgagcttg gcccggtact
      421 cggaagcgat gtcaccttcc tcgcccagct ccttgcggat tgcgtcgagc tgctggcgca
      481 ggatgtagtc cttctggctc ttctcgatcc gctcggcagc gtcgtcacgg accttgcggc
      541 gcaactcgag atcggctagc acctcacgca tccagaccaa gagcatcccg agccgctgac
      601 ggatgtcgag tgcctcgagg agctccacct tctgctccag gttgaggtcg ggggagtaga
      661 cggcaaagtc tgcgagctgg gatgggttgt cgaccgcgag gagacgctcg gcgactccgg
      721 ccatgccccg gttctcgagc acggcgctga ccaccgcccg gtactccgca accaatgcgt
      781 cgaactcgtc gtcgagcatg tcgacttcgg ggtagggctc ggccgtcacc cacagcgccc
      841 ctgtctcgtc caccgaccct gccccgaccc gggccctggc caccccggaa accacgacgt
      901 tgccgttgtc gtcggactgt tggatctcgg cgatggtgcc caccgctgca tacctgcctt
      961 cgagacgcgg gaccaaccgg agacgcccgt tgctcttggc caccgcggcc agggcggtct
     1021 tgccttcctc ggtctcgatg cgcagggtca cgaccatgtg ggggaagacc acgccgttgt
     1081 gcagggggag aacgggaagc gtcagttggg cggtttcagg catcaggacc ggtccttcgg
     1141 taagtgagcc tgtgccaacc cctggttgtc gcagcctatt ccagatctga gccgcttcta
     1201 ctcaggtttg ctcagccttc gctattgcgg ggaatgggca atcagagact gccggccacg
     1261 atgccacagc agattgcgat cgtcgccgag accagccccc accaacgctc cggcttcgat
     1321 ctcgaagcga gattggcgat ggtcccaacc gtgaacagcc cggtgagtat ccacatgccg
     1381 gtggcaccca gctttggtat cgggtcgcgg gcatcgatcg caccacccgc ggtcagaacc
     1441 aggaggatga tgatcggata caggaacact ccggcgatcc cgctcgccac ccgaagcccc
     1501 ggcggcagct ttccctcgtg tctcccgccc caggcggcgc gacctgcagg caggcccagg
     1561 gccaatgcga tttggaaaac ggagacgaca ccgagcaaga ccgcggcgat gatcgatgcc
     1621 gtggtcaaga ccgggccagg actgctctac cgggcgacga ccctccgctc aatggtcctc
     1681 ggcggcctcg gcgtcggcct tggtccggtg cacggtgcca tcggg
//
LOCUS       DATITW010000469         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_583910, whole genome shotgun sequence.
ACCESSION   DATITW010000469 DATITW010000000
VERSION     DATITW010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583910"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1256
                     /locus_tag="VLS86_06525"
     CDS             138..1256
                     /locus_tag="VLS86_06525"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HSO50185.1"
                     /translation="MTSIQGTEEQGATFVELFFDLVFVFAVTQVTASLAHDLTAPGLL
                     RALIVFWLVWWAWTQYTWSLNEADTEHGWIQFTTLLAAALAFLMAVTVPLITTEWGWL
                     FALSYLILRMVGIGLQWRLAGDDAVWARSVRTWTLMSSLGLLAIVTAMFLPPERRFVA
                     LGVAALLDVVAALRAGRGEWRLFPGHFSERHGLFVIIALGESLIAAGLTLSDHPLSGR
                     LLMVAVTAVAGSCALWWTYFGWAKDRLEENMKSQSPARIGRYARDVYSFAHFPLIFGV
                     IGYAISVEETIAHPKDPLQLPGLVALCLGVGLIVGGTALALVLAGARVPAIRWWVMIG
                     LVAAIPALGPIPAWVALSLVSLLVAVLALVETRQHPAG"
     gene            1399..>2679
                     /locus_tag="VLS86_06530"
     CDS             1399..>2679
                     /locus_tag="VLS86_06530"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HSO50186.1"
                     /translation="MLIVGGVLANAVIGTAYSTEFALPDVESRRGFDILDEHFAGSSA
                     GGEGGTIVFRTEQGVTDPEVQAAMTEFFDAASQIEGITLVSPYSPEGAQQIASQGPEA
                     GLIAYAQIGVSRDTTLEEASEIRAELDEMMPDVEGLQIEIGGQIFAAFEVPSSELLGL
                     AFAIIVLILAFGSVLAMGLPIGIALAGIGLGTSLVGILSNVLSIPDFATTVALMLGLG
                     VGIDYALFIVTRFRENMHAGASIEESAVVAIDTAGRAVTCAATTVVISLLGMMLMNLA
                     FVTGIAVACATVVEVTAVASLTLLPAMIGFAGERIEVTRWRGLIAALLLAVGLVGVAL
                     AFRPLAIVGLSLAAVVIVARFAISALRREVPRRPPRPIRETVPYRWSRFIQHRPWLSA
                     IAGVVILVTLAFPYLEQRLAFSDEGNYPEETTTRR"
BASE COUNT          375 a          905 c          868 g          531 t
ORIGIN      
        1 tggtccgccg attcgatctc acaggatcat caaaccgaga cccggttccg gtcaatgggg
       61 gatcgacccc acagccgggg cgaagcgagt cgcgacatgg ccggcgcccc ggggccgggc
      121 ccttaggatc cggcgccgtg accagcatcc agggaaccga agaacagggc gcgaccttcg
      181 tcgagctgtt cttcgacctg gtctttgtct tcgccgtcac ccaggtcacc gcgtcgttgg
      241 cccacgacct gaccgccccc ggtctgctcc gggcgttgat cgtcttctgg ctcgtgtggt
      301 gggcctggac ccaatacacc tggtcgctca acgaagcgga caccgagcac gggtggatcc
      361 agttcacgac tctgctcgcg gcggcccttg ccttcttgat ggcggtgacg gtgccactga
      421 tcacaaccga gtggggttgg ctgttcgccc tgtcctacct gatcctgcgg atggtcggca
      481 tcgggctgca atggagactg gctggggatg acgccgtctg ggcccggagt gtgcgcacct
      541 ggaccctcat gtcgagtctc ggtctcctgg cgatcgtgac cgcgatgttc ctcccaccgg
      601 agcgtcgatt cgtcgccctg ggagtggccg ccctgctcga tgtggtcgca gccctgcggg
      661 caggacgggg cgagtggagg ttgtttcccg gtcatttctc ggaacgacac ggactgttcg
      721 tgatcatcgc cctcggcgag tccctgatcg ccgcaggtct caccttgagc gaccacccgc
      781 tgagcgggag actgctcatg gtcgcggtca ccgcggtcgc cggttcgtgt gccctgtggt
      841 ggacctactt cggctgggcc aaggaccgtc tcgaggagaa catgaagtcg cagagcccgg
      901 cgaggatcgg tcgatatgcc cgggacgtgt actcctttgc ccactttccc ctgatcttcg
      961 gggtgatcgg ctacgccatc agcgtcgagg agacgatcgc gcatcccaaa gacccgctcc
     1021 aactcccggg gctggtcgca ctctgtctcg gcgtcggtct gatcgtgggc ggcaccgcgc
     1081 tcgcattggt cctggccggg gctcgggtcc cggcgatcag atggtgggtg atgatcggcc
     1141 tggtggctgc gatcccggcg ctcggcccga tcccggcgtg ggtggcgctg tccctggtgt
     1201 cgctcctggt cgcggttctg gccctcgtcg agacccgcca gcacccagcc ggctgacact
     1261 cgccgaaatc acgagggcgg ctggacagcg actcgccact agtaacctcg cacaccttca
     1321 aaaggagtct tcttgttcgc tcgacttgga cgttggtgtt tccggaggcg gggccgagtc
     1381 gccctcggct ggctcggggt gctcatagtc ggtggggtgc tcgccaacgc ggtgatcggc
     1441 accgcctaca gcaccgagtt cgcccttccc gacgtcgaaa gccggcgcgg cttcgacatc
     1501 ctcgacgagc attttgctgg ttcctctgcc ggcggcgagg gcggcaccat cgtgttccgg
     1561 accgagcagg gggtcaccga ccccgaggtc caggcggcga tgaccgagtt cttcgacgcg
     1621 gcgagccaga tcgagggaat caccctggtc agcccctact cacccgaagg cgcacagcag
     1681 attgcatccc aagggcccga ggccggcctg atcgcctatg cccagatcgg agtctcccgc
     1741 gacacgacgc tcgaggaagc ctctgagatc agagccgagc tcgacgagat gatgcccgac
     1801 gtcgaagggc tccagatcga gatcggtggt cagatcttcg ccgctttcga ggtgcccagc
     1861 tcggagctgc tcgggcttgc cttcgccatc atcgtcctga tcctggcctt cgggtccgtc
     1921 ctggccatgg gtctgccgat cgggatcgcc ctggccggca tcgggctcgg cacatccctg
     1981 gtcggcatcc tgagcaatgt cctctccatc cccgacttcg ccaccaccgt cgccctcatg
     2041 ctcggtctcg gtgttgggat cgactacgcc ctgttcatcg tcacccgctt ccgggagaac
     2101 atgcacgccg gcgcctcgat cgaggagtcg gcggtggtcg ccatcgacac ggctggtcgg
     2161 gcagtcacct gtgccgcgac gacagtcgtc atctccctgc tcggcatgat gctgatgaat
     2221 ctggctttcg tgaccgggat cgccgtcgcc tgcgccaccg tggtagaggt cactgcggtg
     2281 gcttcgctca cgctgcttcc cgcaatgatc ggcttcgccg gcgagcgcat cgaggtgacg
     2341 cggtggcgtg gcctgattgc ggccctcctc ctcgccgtcg gtttggtcgg cgtcgcactt
     2401 gccttccggc cgctcgccat cgtcgggctg tccctcgccg ccgtggtcat cgtggccagg
     2461 ttcgccatct ccgctctgcg gcgcgaggtg ccacgacgcc ctccgcgtcc gatccgggag
     2521 acggtgccct atcgatggag ccgcttcatc cagcatcggc cctggctgtc tgccatcgcc
     2581 ggtgtggtga tcttggtgac gctggccttc ccctacctcg aacagcgtct tgctttctcc
     2641 gatgagggca actacccgga ggagacgacg actcggcgt
//
LOCUS       DATITW010000470         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_623887, whole genome shotgun sequence.
ACCESSION   DATITW010000470 DATITW010000000
VERSION     DATITW010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623887"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VLS86_06535"
     CDS             complement(<1..1142)
                     /locus_tag="VLS86_06535"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSO50187.1"
                     /translation="MPFGVGLVGLLLTLGMFVALWNAADQGAEERLATQALTVVQSAS
                     DALSTVDARLVSLSGLFRASIAVTPVEFEQFTDDVGLEPGMAGIGYLLRIDSSQLEVT
                     EALLAVETGSEVTAYEFDSEGHPAPVGSRREYHLVQHVSPRDEWGQLVGLDLGSISLI
                     EPALTAAVESRQSAMTSLFDLPGQVDGDNFAMFRVVTSPSTDEPVAVAVAMMDFSDLI
                     EANIPAGVAPYVQWSFAEMGDDLSGLSATTVELGHGGQAFAISVSAIPDSPFAADREG
                     AFVALGLGLLASCLAVLSMLLIGQRIESAADLAGAQSMTEAKDRFIASISHELRTPLT
                     SVLGFAEILRDGEELSAAERMAMMKAITEEATDLAHIIDDLLVAARG"
     gene            1306..>2058
                     /gene="ppk2"
                     /locus_tag="VLS86_06540"
     CDS             1306..>2058
                     /gene="ppk2"
                     /locus_tag="VLS86_06540"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HSO50188.1"
                     /translation="METSGELPAGIDDAVRLGTPAGAGNYSEDGRLKKKFYEAELVRL
                     QEELVKLQYWVANQGLRVLIIFEGRGSAGKGGVIKRITEQTSPRHVRLVALPRPTERE
                     RTQWYFQRFVAHLPAAGEIVLFDRSWYNRSNVEWVMGFCAEAEHEEFLRSCPEFERML
                     VRSGIIVLKYWFSVSWDEQRRRFESRNDEPLKRWKLSDMDLEEHRLYARYSQAKDMTF
                     QFTDIKQAPWYVVPSDDKRRARLNCISHILSHF"
BASE COUNT          401 a          646 c          663 g          348 t
ORIGIN      
        1 ccccgcgccg ctaccaggag gtcgtcgatg atgtgggcca ggtcggtggc ctcttcggtg
       61 attgccttca tcatcgccat tcgctcggca gccgacagct cctccccgtc gcggaggatc
      121 tctgcgaacc cgagcaccga ggtgagggga gtgcgtagct cgtggctgat cgaagcgatg
      181 aaccgatcct tggcttcggt catgctctgg gcaccggcca gatccgccgc ggactcgatc
      241 cgctgcccga tgaggagcat ggacagaacg gccaggcaac tggcgagcag ccccaggccc
      301 agagccacga aggcgccttc gcggtcggcg gcgaacgggg aatcggggat ggccgacacg
      361 gagatggcga aggcctgacc gccatggccg agctcgaccg tggtcgcgct gagcccactg
      421 aggtcatctc ccatctcggc aaagctccac tgcacatagg gggcgactcc ggccgggatg
      481 ttggcctcga tcaggtcact gaagtccatc atcgccaccg ctaccgccac cggttcgtcg
      541 gtcgaggggc tggtcaccac gcggaacatg gcgaaattgt ccccatcgac ctgtccgggg
      601 agatcgaaca gtgaagtcat ggccgactgg cggctttcca cggctgcggt cagcgccggc
      661 tcgatgagtg agatcgaccc gagatcgaga ccgaccagct gcccccactc gtcacggggc
      721 gagacgtgct ggaccaggtg gtactcgcga cgtgacccga ccggggccgg gtggccttcg
      781 ctatcgaatt cgtacgctgt gacctcggac ccggtctcaa ccgcgagcag ggcttcggtg
      841 acctcgagct gggaggaatc gatccgcaac aagtagccga tcccggccat ccccggttcc
      901 aggcctacgt cgtcggtgaa ctgctcgaac tcgaccgggg tgacagcgat gctggcgcga
      961 aacaggccac tcaatgagac cagtctggcg tcaaccgtgc tcagggcgtc agaagccgac
     1021 tggaccacag tgagggcctg ggtcgcaaga cgctcctcgg caccctgatc ggcggcgttc
     1081 cacagggcaa cgaacatgcc cagggtgagc agcaagccga ccagcccgac accgaatggc
     1141 aaccgccgcg ccaacgcgcc acgacgttgg cgaatcatct cccccgagga atgcaacgat
     1201 cctcggtctt tcatgaggta tgtatcggaa tctggcgatg gttgcttgaa ccagcgtccc
     1261 ccccattagc gcgtccccta ggtttgattc cgacgaggag aagctatgga aacctctggc
     1321 gagctccccg ctgggatcga cgacgccgtt cggctcggga cacctgcggg cgccgggaat
     1381 tacagcgagg atgggcggct gaagaagaag ttctatgaag ctgagctcgt ccgactgcaa
     1441 gaggagttgg tgaagctcca gtactgggta gccaaccagg ggctccgtgt gctcatcatc
     1501 ttcgagggtc gagggtctgc gggcaaaggg ggggtgatca agcggatcac cgagcagacc
     1561 agcccccgac acgtgagatt ggtggcgctg ccccgcccga cggagcgaga gcggacacag
     1621 tggtacttcc agcgcttcgt tgcccatctc cccgcggcgg gcgagatagt cctcttcgat
     1681 cggagctggt acaaccgctc gaacgtcgag tgggtgatgg ggttctgcgc cgaggccgaa
     1741 cacgaggaat tcctccgcag ttgccccgag ttcgagcgaa tgctggttcg gtccgggatc
     1801 atcgtcctca agtactggtt ctcggtgagc tgggacgagc agagacgccg gttcgagtcg
     1861 cggaacgacg agcctctgaa gcgatggaaa ctgtcggaca tggacctcga ggagcatcgg
     1921 ctctatgccc gctattcaca agccaaagac atgaccttcc agttcaccga catcaagcag
     1981 gcgccttggt atgtggtccc ttccgacgac aagcgccgcg cccggctcaa ctgcataagc
     2041 cacatactgt cccacttc
//
LOCUS       DATITW010000471         1542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_63236, whole genome shotgun sequence.
ACCESSION   DATITW010000471 DATITW010000000
VERSION     DATITW010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63236"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1096)
                     /locus_tag="VLS86_06545"
     CDS             complement(74..1096)
                     /locus_tag="VLS86_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50189.1"
                     /translation="MLLLAAGCTAPSTTTATTTTTIVTTTATTSTMATTIATTTTPPR
                     AESSGLTPVLIGEPIDLATLRGTIVFDDFEDLFTVDVGGTGLFRLTTQPGSEFDGSWS
                     PDGSMIVYRDSRRGINEDDEIYVISADGSGARNLTQDPSNDWGPEWSADGKWIVFNSD
                     RDGHPMGGYLMRPDGTDVRRIESDTWIEYPSLSPDGDRLVFMGHSGGNYEIYVADLAT
                     GETTALTDSPGSDGWPVWSPDGEWIAFVTERDDCLHAAPDADCWRADESGEHHSTWRM
                     RPDGSEQQRVTPEYGHFVAWSPDSAHLLISGRTLYIVRVDGTGRVEVEPDRLSLPPGG
                     IPDWVG"
     gene            complement(1117..>1542)
                     /locus_tag="VLS86_06550"
     CDS             complement(1117..>1542)
                     /locus_tag="VLS86_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSO50190.1"
                     /translation="AMANSWQGEFPWQNLGLDGFEGTSPVASFPVNGYGLYDMTGNVW
                     EWTTDWYAPQHPEPESDACCAPSNPRVISPDESFDIGRPGENIPRRVIKGGSHLCAPN
                     YCLRYRPAARQPQMIDTSMSHLGFRCISREPRSTPREEI"
BASE COUNT          257 a          457 c          527 g          301 t
ORIGIN      
        1 ttcttgatcc tggcggtggg tgcccgcata ggagccggta ctctcggcag gtcacaaggc
       61 ggcggctttg gtgctagccg acccagtcgg gaattcctcc aggtgggagc gagagcctgt
      121 caggctcgac ctcgactcga cccgtgccat cgacgcgcac gatgtaaaga gtccgtccgg
      181 agatgaggag gtgggcgctg tccggtgacc aggcgacgaa gtgcccgtac tccggcgtga
      241 cgcgttgctg ctccgacccg tcggggcgca tgcgccacgt gctgtggtgc tcacccgact
      301 cgtcggcccg ccagcagtcg gcgtcgggcg ccgcatggag acagtcgtcg cgctctgtca
      361 cgaatgctat ccactcacca tcgggtgacc agaccggcca tccgtcggag cccggcgagt
      421 cagtcagagc cgtcgtctcc cctgttgcca ggtctgccac gtagatctcg tagtttccac
      481 cggaatgacc catgaacacc agccgatcgc cgtcgggcga cagagacgga tactcgatcc
      541 aggtgtcgga ctcgatacgt cggacgtcgg tcccgtcggg tcgcatcaga tagccaccca
      601 tggggtgacc gtcccggtcc gagttgaaga cgatccactt gccgtcggcc gaccactcag
      661 ggccccaatc attgctcggg tcctgagtca ggttcctcgc tccggagccg tcggctgaga
      721 tgacgtagat ctcgtcgtct tcgttgatcc cgcgccgcga gtcccggtag acgatcatcg
      781 aaccgtcggg tgaccaggat ccgtcgaact cagacccagg ctgggtggtc aatctgaaga
      841 gaccggtgcc tcccacatcg acggtgaaga ggtcctcgaa gtcgtcgaag acgatcgttc
      901 cccgcaaggt cgccaggtcg atcggctccc cgatcaggac cggcgtgaga ccactgctct
      961 cggcgcgggg tggtgtggtg gttgtggcga tggtcgtagc catggtggat gtggtcgctg
     1021 tcgtggtgac gatggtcgtt gtcgtggtgg ctgtcgtcgt ggaaggggcg gtgcaaccgg
     1081 cggcgaggag caacaccagg atgagcctgc gaaaggtcag atttcttctc gtggcgttga
     1141 ccgcggctct ctgctgatgc agcgaaaacc caggtgcgac atggaggtgt cgatcatctg
     1201 gggttgtcgg gctgcaggcc ggtagcggag gcagtagttg ggggcgcaca ggtgtgagcc
     1261 acctttgatg accctgcggg ggatgttctc gcccggccgg cctatgtcga agctctcatc
     1321 gggtgaaatt actcgcggat tggaaggggc gcagcaggcg tcggattctg gttcgggatg
     1381 ctgaggggcg taccagtcag tggtccactc ccagacgttg ccggtcatgt catagagccc
     1441 atatccgttc accgggaagc tggcgacggg tgaggtccct tcgaacccgt cgagcccgag
     1501 gttttgccag gggaactcac cctgccagct gttggccatg gc
//
LOCUS       DATITW010000472         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_743813, whole genome shotgun sequence.
ACCESSION   DATITW010000472 DATITW010000000
VERSION     DATITW010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743813"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VLS86_06555"
     CDS             <1..187
                     /locus_tag="VLS86_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50191.1"
                     /translation="LNTMKVRTGKIHTVGAAAPTAAPAPATAPEPAAADEPAADEPVA
                     AEPVLEEIAADAVSEEE"
     gene            184..441
                     /locus_tag="VLS86_06560"
     CDS             184..441
                     /locus_tag="VLS86_06560"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HSO50192.1"
                     /translation="MTLAKGEIVERVVTYVVKSIVEEPDEVSVTMVEQGPDEVVAEVR
                     TAKGDMGRVIGRRGRVAKAIRAAAGAAGDEEGINAGVEFLD"
     gene            455..934
                     /gene="rimM"
                     /locus_tag="VLS86_06565"
     CDS             455..934
                     /gene="rimM"
                     /locus_tag="VLS86_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HSO50193.1"
                     /translation="MVGRLGRPHGLDGFLGLYIEEEDAATIQPGTTLFVNDRPLVVRA
                     LRRVDRGYQVAFEGFEDRVSAEEIRGGDVLMEERRELGEGEYWPGDLIDLQVFDQEGS
                     RLGKVIDVLFGPGQDRLLVETETGATFEVPFVGDLVPSVDIAAGRIDILSIPGLTES"
     gene            complement(921..1793)
                     /locus_tag="VLS86_06570"
     CDS             complement(921..1793)
                     /locus_tag="VLS86_06570"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HSO50194.1"
                     /translation="MRLPALLALVLIVACMPAPGGEVTTTSPETTTTSISAATTSSAV
                     PTTQADVACPPTTDFVDRGRVLASDQPTSDTNSLGLVTWQVEAGCERFEIEFETNEGA
                     PATTPPTVVVEFLASRQILRIRTDVGSTVITDQLVETPLVDRLFVVRALDGGMFVDFH
                     LAGPSAARVSISGSPARLTLELQPAVDPIPAPAVISDRVVVLSPGDGTEAGARVEVSG
                     YARTFEANVLVIATSGDQVVANANLTAADWAETWGEFRTTLPLPPGEVSIFVGEESPE
                     DGQLSGATVVVTIQ"
     gene            1876..>2637
                     /locus_tag="VLS86_06575"
     CDS             1876..>2637
                     /locus_tag="VLS86_06575"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HSO50195.1"
                     /translation="MSEAVIVATARSPIGRAYKGSLIDVRADDLAGFAINAVMEKVPE
                     VERASVVDVMVGCGLTHHEQGFNLARPASLLGGMPDSVPATTVNRFCASSLQTIRMAF
                     HAINQGEGDTYLAAGTESVTRTYGKAFDSDDLNPRFSDASRDDFINHVYIPMGNTAEN
                     VAAKYGITRERMDEFAKRSQDLAVESQKNGFFDWEITPIEVPDGRTISADDGPRADTT
                     LEKLASLPPVFKPDGSVTAGNSCPLNDGAAAVLVMS"
BASE COUNT          472 a          825 c          915 g          425 t
ORIGIN      
        1 cctcaacacg atgaaggtcc gcaccgggaa gatccacacg gtcggtgccg cggctccgac
       61 cgctgctccc gccccggcaa cggctccgga gccggctgct gccgatgaac cggctgccga
      121 cgagcctgtc gccgccgaac cggtgctgga agagatcgcc gccgatgcgg tctccgagga
      181 ggaatgacat tggccaaggg cgaaatcgtc gagcgagtcg tcacctacgt cgtcaagagc
      241 atcgtcgagg agccggatga ggtctcggtg acgatggtgg agcagggccc cgacgaagtg
      301 gtcgccgagg tccggaccgc aaaaggtgac atggggcggg tcatcgggcg acggggccgg
      361 gtggccaaag ccatccgcgc cgcggccggc gcggccggcg atgaagaagg cataaacgct
      421 ggcgtcgaat tcctcgactg atccgcagcg attcgtggtc ggccggttgg gccgccccca
      481 cggtctggac gggttcctcg gtctctacat cgaagaagag gacgcggcca cgatccagcc
      541 cggcacgacg ttgttcgtga acgatcgtcc gctcgtggtg cgtgcgcttc ggcgcgtcga
      601 ccggggatat caggtggcct tcgagggctt cgaagaccgg gtcagcgccg aggagatccg
      661 tggcggcgac gtcctcatgg aagagcgcag ggagctgggc gagggcgagt actggcccgg
      721 tgacctgatc gacctccagg tgttcgacca ggaggggtcg aggttgggca aggtgataga
      781 tgtcctcttc ggccccggtc aggaccggct gctggtcgaa accgagactg gcgccacgtt
      841 cgaggtgccc ttcgtggggg acctggtgcc gtcggtggac atcgccgccg gccgaatcga
      901 catcctctcc atcccggggc tcactgaatc gtgaccacca ctgtggctcc gctgagctga
      961 ccgtcctccg ggctctcctc gccgacgaag atcgatacct cgccgggtgg caacggcagg
     1021 gttgttcgga actcgcccca ggtctccgcc cagtcggccg cggtcagatt tgcgttggcc
     1081 acgacctggt caccggaagt cgcgatgacg aggacgttcg cctcgaacgt cctggcatag
     1141 cccgacacct cgactcgagc gcctgcctcg gtgccgtccc cggggctgag cacgaccacc
     1201 cggtcgctga tcaccgccgg agcgggaatc gggtcgacgg cgggctgcaa ctcgagggtc
     1261 agccttgcgg gggagcccga gatcgacact ctcgccgcgg aggggcctgc gaggtggaag
     1321 tcgacgaaca tccccccgtc gagggccctg accacgaaga gacggtcgac gagaggcgtc
     1381 tcgaccagtt ggtcggtgat caccgtcgaa ccgacgtcgg tacgaatccg gagaatctga
     1441 cgtgacgcca ggaactcgac gacgacggtg ggcggggtgg tggccggcgc accctcgttg
     1501 gtctcgaatt cgatctcgaa ccgctcgcac ccagcttcga cctgccaggt gacgagaccg
     1561 agactgttgg tgtcggaggt tggctggtcc gatgccagca cccggccacg gtccacgaaa
     1621 tccgtcgtcg gggggcaggc gacatcggct tgggtcgtgg gcaccgccga gctggtggtc
     1681 gcggctgaaa tgctggtggt ggtcgtctct ggcgacgtcg tcgtcacctc gccaccgggg
     1741 gcaggcatgc aggccacgat caggaccagg gcgagaaggg ctggcagccg catgtcggaa
     1801 agggtacgga ccctcattag attcgagcag ataatcgagg tccggtcgat cccgggccaa
     1861 ttggagtgat ccgcaatgtc cgaagccgtg atcgtcgcca ccgcccgttc cccgataggg
     1921 cgcgcctaca agggatcgct gatcgatgtc cgagccgacg acctggccgg gttcgccatc
     1981 aatgccgtca tggagaaggt gcccgaggtg gagcgggcgt cggtggtcga cgtgatggtc
     2041 ggatgcgggc tcacccacca cgagcagggc ttcaacctgg ctcgtcccgc atcgcttctc
     2101 ggcggtatgc ccgacagtgt gccggcgacg acggtcaatc gattctgcgc ctcctccctg
     2161 cagacgatcc ggatggcgtt tcatgcgatc aaccagggag agggcgacac ttatctggca
     2221 gccggcaccg agtcggtgac ccggacctat ggcaaggctt tcgacagcga tgacctgaac
     2281 ccgcggttca gcgacgcgag ccgggatgac ttcatcaacc acgtgtacat cccgatgggt
     2341 aacaccgcgg agaatgtcgc agccaagtac gggataacga gagagcgaat ggacgagttc
     2401 gccaagagat cacaggatct cgcggtcgag tcgcagaaga acggcttctt cgactgggag
     2461 atcaccccga tcgaggtgcc cgacgggcga acgatcagtg ccgacgacgg gccccgggcg
     2521 gatacgaccc tggagaagct ggcctctctg cctccggtct tcaaacccga tgggagcgtc
     2581 accgccggga actcgtgccc cctcaacgat ggcgccgcag ctgtcctggt gatgtcc
//
LOCUS       DATITW010000473         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_182977, whole genome shotgun sequence.
ACCESSION   DATITW010000473 DATITW010000000
VERSION     DATITW010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182977"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VLS86_06580"
     CDS             <1..201
                     /locus_tag="VLS86_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50196.1"
                     /translation="KIGQISLFRMSSPAEHPYGSNRLGSKYLDQVGPTPSRYWENFET
                     QTETQLTETQLAETQPDGPSSK"
     gene            complement(174..983)
                     /locus_tag="VLS86_06585"
     CDS             complement(174..983)
                     /locus_tag="VLS86_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSO50197.1"
                     /translation="MAISDRWQELGDGIFRRRYEFLDQNIGLVIGAEAVLVIDTRSHP
                     GHAEELLSDLRFVTPLPVGWVFNTHYHWDHAFGNQVFRTSTIWGHVNCRMELIERGLT
                     PLDELLVDYPEESDGFQRVVVTPPTEVFEERARIDLGNRSVEVSYHGQGHTNSDAVLH
                     ADDVTFAGDLIEEGAPPSFGDSFPIAWFDTVGRLAAEARTIVVPGHGDVVDVEYLVVS
                     RFDLGWIARQARLALDAGLTVEQIDLKGSPYEEYYAREALERAYLELGPSG"
     gene            complement(1251..>2296)
                     /locus_tag="VLS86_06590"
     CDS             complement(1251..>2296)
                     /locus_tag="VLS86_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HSO50198.1"
                     /translation="VGELDRLINWLDSKASDRDAMKASEFMADLVAVRKVAGEGSTID
                     VLDEIRHRVGSDGLEQSASSLDGWSHGAVASHRDDLDALMVLGHLQPDPARFGAWLAD
                     ALSTPDDPAGVTLASIHAVKGREWEHVIVHDVTAGLMPHRLVDGIEEERRVFHVGLTR
                     GIASVTLVPGTPPSPFLAEMNEPGSPVPEPAPMRLVPSRPVKKTAADPAAAVDPVEAQ
                     TGLCFDHGGYLHEVVDLDPDGAITRVGDEGARLTVEFGTVVRVLGVPRRLAHPNHLMA
                     FERFRAWRTQRANGKPAYTVLSDETLRALAIGLPADEASLARVKGIGPMKLEAFGEEL
                     LELMAEMRSTKSG"
BASE COUNT          465 a          727 c          691 g          413 t
ORIGIN      
        1 aagatcggac agatcagcct cttccggatg agttcaccag ccgagcaccc ttatggatcc
       61 aaccggttgg gaagcaagta cctggaccag gtcggtccga ctccttctcg atattgggag
      121 aacttcgaga cccaaactga aactcaattg accgaaacac aattggccga aactcagccc
      181 gacggaccca gctccaagta ggcgcgttcc agagcctctc gggcgtagta ctcctcgtag
      241 ggcgatcctt tcagatcgat ctgctccaca gtcaggccgg catcgagggc gagacgggcc
      301 tgacgggcga tccagccgag gtcgaacctg cttacaacga ggtactcgac gtcgacgaca
      361 tccccgtggc ccggaaccac gatcgtgcga gcctcggccg ccaatcgccc gacagtgtcg
      421 aaccaggcga tcgggaacga atcgccaaag ctgggcggag ctccctcttc gatcagatcg
      481 ccggcaaagg tcacgtcgtc ggcgtggaga acagcgtcgc tgttggtgtg accctgaccg
      541 tgatacgaca cctcgacact gcggttgccg agatcgatcc tggctcgctc ttcgaagacc
      601 tcggtcgggg gagtcaccac aactctctgg aatccgtctg actcctccgg gtagtcgacc
      661 agaagctcgt cgagaggggt caagcctctc tcgatcaact ccatccggca attgacgtgg
      721 ccccagatgg tcgaagtcct gaagacctgg tttccgaagg cgtgatccca gtgatagtgg
      781 gtgttgaaca cccatccgac cggaagcggg gtgacaaaac ggaggtcgga gagcaactcc
      841 tcagcatgac cgggatggga ccgggtgtcg atcacgagga cggcctcggc tccgattacc
      901 aggccaatgt tttgatcgag gaactcgtac cggcggcgga agatgccatc accgagttcc
      961 tgccaacggt cagaaatggc catggggcga ccctactagc ggactagtta gtccgagcga
     1021 cctagatatg aatctcaaac aacgtgtcat gctcaccgat accaggcgga aagcatcaga
     1081 ttggtgaccg gtcgaaatct ctcctccttc cagagagact cttcccaaga tcctccatcg
     1141 ggcggttacc agggatggaa agcggctcgc aagagccgct ttccgccttt ttcggcgagc
     1201 ttctctcggt ctttgaacag acctgcagcc gattggccgt tctctagccg tcaacccgac
     1261 ttcgtagacc gcatctcggc catcagctca aggagctcct ctccaaatgc ctcgagcttc
     1321 atcgggccga ttcccttcac ccgggccaga gaggcttcgt cagccggaag accgatcgcc
     1381 agcgcccgca gcgtctcgtc ggaaagaacg gtgtaggcgg gcttgccgtt ggccctttgg
     1441 gttctccagg ctcggaaccg ctcgaaggcc atcagatggt tggggtgcgc cagccgacgg
     1501 ggaactccga gcaccctcac gacggttccg aactcgaccg tgaggcgggc tccctcgtcg
     1561 cccaccctgg ttatggcacc gtcggggtcg agatcgacca cctcgtggag atagccgccg
     1621 tggtcgaagc acaggccagt ctgcgcctca accggatcaa ccgccgccgc cggatcagct
     1681 gcagttttct tgaccggccg ggaggggacc aacctcatcg gagcaggctc tggaaccggg
     1741 gagcctggct cattcatctc agcgaggaag ggggaaggag gcgtgcccgg cacgagtgtc
     1801 acgctcgcga tgccgcgggt taggccaacg tggaaaaccc ggcgctcctc ctcgatgcca
     1861 tcgacgaggc ggtggggcat caatccggcc gtgacgtcat gcacgatgac gtgttcccac
     1921 tctcgaccct tgaccgcgtg aatcgaggcc agggtcactc cggccgggtc atcgggagtg
     1981 ctgagggcgt cggccaacca tgccccgaag cgggcgggat cgggctgaag atgtcccagg
     2041 accatcaggg cgtcgaggtc atcacgatgt gatgccaccg ccccatgact ccagccgtcg
     2101 agcgaggagg cactctgctc gagaccgtcc gaacccactc gatggcgaat ttcgtcgagg
     2161 acatcgatgg tcgagccctc cccggcgact ttccgcacgg ccacgaggtc ggccatgaac
     2221 tcgctggcct tcatggcgtc gcggtcgctc gccttgctat ccagccagtt gatcaggcga
     2281 tccaattcgc cgacgg
//
LOCUS       DATITW010000474         1826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_544163, whole genome shotgun sequence.
ACCESSION   DATITW010000474 DATITW010000000
VERSION     DATITW010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544163"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VLS86_06595"
     CDS             <1..514
                     /locus_tag="VLS86_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50199.1"
                     /translation="DRLHDSVLQTLHAIRMGADDPSQSRYLARRQERELRRNIEEWRS
                     EYRQSFRASLLAVRDEVEDTHRVEIEAVIRDDAQINPSLAAAVEAAREAMANAAKHSG
                     SPNITLYSEFANGEAHIHVRDSGSGFTDEAQKTRVHERLARRVEGVGGLVEIDTAPES
                     GTEVKITVGK"
     gene            511..1161
                     /locus_tag="VLS86_06600"
     CDS             511..1161
                     /locus_tag="VLS86_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSO50200.1"
                     /translation="MTDRLRIIIVDDHDVVRAGIRAILDSSFDIVGEADNVDSAIELI
                     KERLPDLVVLDVKLPGGGGATVIPAVRRHFPDIRFMALTVSTSKEDVARLLDAGVDGY
                     VTKTTLGADLPDLIRQTYVGARPVSPDVAAYLLDIDEEITSESAISRLTPREREVVNL
                     IARGYTYRESAERLGITVKTLENHMAHIFDKLSVASRHELTALAYEEGYVRPDDER"
     gene            complement(1437..>1826)
                     /locus_tag="VLS86_06605"
     CDS             complement(1437..>1826)
                     /locus_tag="VLS86_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HSO50201.1"
                     /translation="GTVLTLNVGMERVRIVEWLEDDPYPMALVERLEDGVATAAVESV
                     VEDCRLLLASVRDLAAELGAEVGVEPSLSDDPIRATFQIASLAPLQAFDQQKVLEADD
                     PLTRAQTLRAELVGMVEMLTLELAVGR"
BASE COUNT          353 a          630 c          576 g          267 t
ORIGIN      
        1 cgaccggctc cacgactcgg tgcttcagac cttgcacgca attcgcatgg gcgcagacga
       61 cccgagccag tccaggtacc tggcccgtcg ccaggagcgg gagttgcggc gaaacatcga
      121 ggaatggaga tcggagtacc gccagagctt ccgagccagc ctgcttgcgg ttcgcgacga
      181 ggtcgaggac acccaccggg tcgagatcga agccgtgatc agggacgacg cccagatcaa
      241 ccccagtctc gccgcggccg tggaggcggc gcgggaggca atggcgaacg cggccaagca
      301 ctccgggtcc cccaacatca cgctgtactc cgaattcgcc aacggcgaag cccacatcca
      361 cgtgcgcgac agcgggtccg gtttcaccga cgaagcccag aagacccgcg tccacgagcg
      421 gctcgcccgg cgggtggaag gtgtgggagg tctggtggag atcgacaccg cgcccgagtc
      481 cgggaccgag gtcaagatca cggtgggcaa gtgaccgaca ggcttcgcat catcatcgtc
      541 gacgaccacg acgtggtccg ggccgggatc agggccatcc tcgacagctc gttcgacatc
      601 gtcggcgaag cggacaacgt ggacagtgcc atcgagctga tcaaggaacg gctaccggac
      661 ctggtggtcc tcgacgtgaa gctgcccggc gggggcggag cgaccgtgat cccggcggtg
      721 cgacgccact tccccgacat ccgattcatg gcgctcaccg tctccacctc caaggaggac
      781 gtggcacgac tgctcgacgc gggagtcgac ggatacgtga ccaagaccac tctcggcgcc
      841 gacctccccg acctcatccg acagacctat gtcggggcaa gaccggtgtc ccccgacgtg
      901 gccgcctacc tcctcgacat cgacgaggag atcacctctg agtcggccat ctcccgtctc
      961 acgccacgcg agcgtgaggt ggtcaatctc atcgcccgtg gctacacgta tcgggagagt
     1021 gccgagcggc tggggataac ggtgaagaca ctggagaacc acatggccca catcttcgac
     1081 aagctgagcg tggccagccg tcacgagctc accgctctcg cctatgaaga gggttacgtc
     1141 cgccccgacg acgagcgctg aaagtcccct cgacttccac ctccacctcc acgtaattgg
     1201 gagccacagg tccggatatg acacctgtct ctcccaattt ggggaggggg acacgtcagg
     1261 ggggattccc cctattcaca gcaccgctct cggccgctac caatgcgctg ggtggggatg
     1321 aggaaacctt cctcccggag ccacatcccc ggaggggagt aggaagccat cacaggctct
     1381 cctggaaacc ggcaagcccc cgtcgtcaga cgggggcttg cgcgctggcc ggggtatcag
     1441 cgaccgacgg cgagctcgag cgtcagcatc tccaccattc ccaccagctc cgcccgcaga
     1501 gtctgggccc gcgtcagcgg atcgtcggct tcgagcacct tctgctgatc gaaggcctga
     1561 aggggcgcca gagaggcgat ctggaacgtg gcccggatcg ggtcgtcgga cagggacggc
     1621 tcgacgccga cctccgcccc cagctctgcg gcgaggtcgc gcaccgatgc caggagcaaa
     1681 cggcaatcct cgaccaccga ttcgacggcg gccgtcgcga ccccgtcctc cagacgctcg
     1741 accagcgcca tcgggtaggg atcgtcctcg agccactcga caatccgcac ccgctccatg
     1801 cccacgttca gggtgagcac cgtgcc
//
LOCUS       DATITW010000475         1809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_23481, whole genome shotgun sequence.
ACCESSION   DATITW010000475 DATITW010000000
VERSION     DATITW010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23481"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VLS86_06610"
     CDS             <1..334
                     /locus_tag="VLS86_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50202.1"
                     /translation="ARMAGGAALLVLYGVGRGGFAGLTGVTTEHVVWILLTGLTLSAY
                     VATWFAALSRAPAVDVTAVLAGGAIITALLELGLRGVELPPVPGLVLVALGAIVVAVA
                     GWRRPAVA"
     gene            334..1089
                     /locus_tag="VLS86_06615"
     CDS             334..1089
                     /locus_tag="VLS86_06615"
                     /inference="COORDINATES: protein motif:HMM:NF040399.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HSO50203.1"
                     /translation="MSDMSGTALFCRFAFPPNSLGYCGPSDPGLLKELVVGGEAAREE
                     VTHVIPAFAGAWPYLELIASCSGRDPLHAAVVEAYWLGNPLLEEVDLHTMGNSVDDRF
                     RRRAGWDWQSVTDALNAGGRPTHSFHVFCIYPWVGLLQSGIVEQALDVLDRCRIRWGE
                     VKGWAGDRLLVDSQPLAWDGARLGLSPGRLESVLPPVDDGRIEVGDLVAMHWDYVCQK
                     ITPQQHSTLRRYHDLHLALANRSRAALGPRIES"
     gene            1208..1591
                     /locus_tag="VLS86_06620"
     CDS             1208..1591
                     /locus_tag="VLS86_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50204.1"
                     /translation="MPGSNGEQTAGGAGEDRLEPLVERVRVAVLVGPGVTQRSLRQAV
                     EERAAALSGRIRASGETGTIPADLSEFVDRVATEAWWVTDEDVAALLEAGYTEDEVFE
                     VSVSAAMGAACGRLERGLAALRGEV"
     gene            1591..>1809
                     /locus_tag="VLS86_06625"
     CDS             1591..>1809
                     /locus_tag="VLS86_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylhydroperoxidase AhpD family core
                     domain-containing protein"
                     /protein_id="HSO50205.1"
                     /translation="MRLDVVERGHRIPQKLQLMMIRLFSGRRAVDIVRTVMYRPEFFG
                     RPMSEWTQKVMRGPSDWAVWERELFAAFT"
BASE COUNT          252 a          565 c          662 g          330 t
ORIGIN      
        1 ggcccgcatg gcgggagggg cggccctcct cgtcctctac ggcgtcggac gtggtggctt
       61 cgccgggctg accggcgtca caaccgaaca tgtggtctgg atcctcctca ccggtctgac
      121 cctgtccgcc tacgtcgcca cctggttcgc cgccttgtct cgtgccccgg ccgtggacgt
      181 caccgccgtg ctagccggcg gggccatcat caccgctcta ctggagctgg gactccgggg
      241 cgtcgagctt cccccggtgc ccggtctggt gctggtggct ctgggtgcga tcgtcgtggc
      301 cgtggccggg tggcgtcgcc cagcggttgc atgatgagcg acatgtccgg gaccgccctg
      361 ttttgccggt tcgcctttcc ccccaactcc ctgggctact gcggtccatc ggaccccggt
      421 ctgctcaagg agctggtcgt cggtggtgag gcagccaggg aagaggtgac tcatgtcatc
      481 ccagccttcg ccggcgcctg gccctatctg gagctgatcg cctcctgcag cggccgggac
      541 ccgctccacg cggcggtggt ggaggcctat tggctgggca accctctcct cgaggaagtc
      601 gacctgcaca caatgggcaa ctccgtcgac gatcgcttcc ggcgccgagc cggatgggac
      661 tggcagtcgg tcaccgacgc cctcaacgcg ggcggtagac ccacccactc ctttcacgtc
      721 ttctgcatct acccctgggt gggcctgctc cagtcgggga tcgtcgagca ggcgctcgac
      781 gtgctcgatc ggtgccgaat ccgttggggt gaggtcaagg ggtgggccgg tgaccggctg
      841 ctggtcgact cacaacccct ggcctgggat ggggcgcggc tcggtctgtc tccggggcgg
      901 ctcgagtcgg tgctgccgcc ggtcgatgac ggtcgcatcg aggtggggga cctggtcgcc
      961 atgcattggg actacgtctg tcagaagatc accccgcagc agcattcaac gctgcggcga
     1021 tatcacgacc tccatcttgc tctcgccaac cggtcccggg cggcactggg accgcgaatc
     1081 gagagctgag gtgggcgttg gccatgtcct gcccaaggcg ataaacgttg cttgcaccga
     1141 ctccttggct gcgatgatct cgtgcctgtt ttgacgccga tacagtgcgg cggccgagga
     1201 ggatgaaatg ccgggttcga acggagagca gaccgcgggc ggagccggcg aagaccggct
     1261 cgagccactc gtggagcgag tgcgtgtggc ggtgttggtc ggtcccggtg tcacgcagcg
     1321 atcgttgcgc caggcggtag aggagcgagc tgcggcgctg agcggccgga tccgagcatc
     1381 gggcgagacc ggcacgattc cggccgatct ctccgagttc gtcgaccggg tagcgaccga
     1441 ggcgtggtgg gtgaccgacg aggatgtcgc agcgctgctc gaggccgggt acacggagga
     1501 cgaggtattc gaggtctcgg tgagcgcggc gatgggcgcg gcctgcggcc ggctggagcg
     1561 aggtctggcg gcgctgaggg gagaagtctg atgcgtctgg acgtggtcga acggggccat
     1621 cggatccctc agaagctgca gctgatgatg atccggctgt tctccgggcg ccgggcggtg
     1681 gacatcgtca ggacggtgat gtaccgaccg gagttcttcg ggcggcccat gagcgagtgg
     1741 acccagaagg tgatgcgggg cccatccgac tgggccgtct gggagcggga gttgttcgcg
     1801 gccttcacg
//
LOCUS       DATITW010000476         3923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_704395, whole genome shotgun sequence.
ACCESSION   DATITW010000476 DATITW010000000
VERSION     DATITW010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704395"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VLS86_06630"
     CDS             complement(<1..421)
                     /locus_tag="VLS86_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50206.1"
                     /translation="MNDTTVRLANSERLDTRRSGFSRWAPLVFFAFILIGPIFSPDAG
                     PEDWILAVGVVAVALPTFVVSEIRGWDIGGATVLMLLALATAPFDSTAIMALPIYAAA
                     LVARSATRETLRFRLSVITVLTLTGFFLSSIPWPFRVF"
     gene            complement(418..1140)
                     /locus_tag="VLS86_06635"
     CDS             complement(418..1140)
                     /locus_tag="VLS86_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO50207.1"
                     /translation="MNTRIFRTEVRDELLGIVREPTALFFSVLMPVAFFALFATMFGG
                     DGGFGVESLATYGAFGVLSVVLTNPGIGLADARERGWLRVKRADGTPLGVTLSAKVTA
                     ALPYAIVTLAGITLVARLTAGPLDVLQVLRVVGVLVLGSLPFSLFSLAVGARFSTNAA
                     TSVLNAVLIPSVILSGLWFPLEMLPTWLGEIAVYLPPYHLAQLALAQVEGGAVWGHVA
                     YLVGTTIVGAIAAALAYRSAEV"
     gene            complement(1137..2033)
                     /locus_tag="VLS86_06640"
     CDS             complement(1137..2033)
                     /locus_tag="VLS86_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50208.1"
                     /translation="MEHLLEVRGLVHRYGDYTALAGVDLTVDSGECVALLGPNGAGKT
                     TLVRNVIGLIEGNADLIEVAGGNPRSAETRRRLGVVQQAVGFPRSLTVGEIVGGAAAR
                     RGCPPEAASQAIAEMGLAGLEKRRADKLSGGQQQRLQLAMGLAADPVLLVLDEPTEGL
                     DVEARRRFWNTIRRRLDNGAGVLITTHLVDEAAAVADRVVVVDRGRVVAEGTPDELRR
                     TLPDRRIEVQTKVPVSVIRALDSVEAVELRDGRTVIATTDSEQVVRTLLDLDPEANDL
                     TVATASLEEVLIAMTHHEEVLI"
     gene            2920..3852
                     /locus_tag="VLS86_06645"
     CDS             2920..3852
                     /locus_tag="VLS86_06645"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HSO50209.1"
                     /translation="MRFRRIAALVTVMALSVAVASCGGDEATTSTTGATVTTEAPATT
                     EAPATTEAPATTEAPATTAAPPAEILLDAPLVTGFGVDGDPGEWADVPSLDLTLEPIG
                     GETLEAKNAVVKVAHDGESVYVLFQVEDAYNWVLGEDPHFTSAVGVQWAIDQSAGGAM
                     GATDDDRETSLGTVDIWHWELECAAGEVSGGATSGPGEGSDPGDDGACNFDDEWSTDP
                     ETREDDNGDGAENSLSGSWSHSDPTADAAGTWTFEMSRPLDTGDSTDAAFAAGESGLL
                     ALAYWDADATPEGWEDDGHVQSANLGWINVTFAS"
BASE COUNT          772 a         1208 c         1267 g          676 t
ORIGIN      
        1 tgaacacccg aaacggccaa gggatcgagc tcaagaagaa gccggtcaga gtgaggacgg
       61 tgatcaccga cagtctgaat cggagcgtct ctcgagtggc tgacctggca acgagagcgg
      121 cggcgtagat aggcagcgcc atgattgctg tcgagtcgaa gggtgcagtc gcgagtgcca
      181 gcagcatcag gacggttgct ccaccgatat cccagcctcg tatttccgag acgacaaagg
      241 tggggagggc gacggcaacc actccgaccg ccaagatcca atcctcggga ccggcgtcgg
      301 gggagaagat cggtccgatg aggatgaatg cgaagaagac caggggtgcc caacgcgaga
      361 aaccggaacg cctcgtgtcg aggcgttccg agttcgcgag tctgactgtc gtgtcgttca
      421 cacttccgcg gaacggtacg ccagcgctgc tgcgatcgct cccacgatcg ttgttcccac
      481 gagataggcg acgtgacccc acactgctcc gccttcgacc tgcgccagcg ccagttgtgc
      541 caggtgatac ggcgggaggt acaccgcgat ttctccgagc catgtcggga gcatctcaag
      601 cgggaaccag aggcctgaga gaatcaccga aggaatgagc accgcgttga gcaccgacgt
      661 tgcggcgttg gtactgaacc tcgcgccaac agccagggag aacaggctga atggcaagga
      721 tccgagtacc agcacaccga cgacacgcag gacctgaagc acgtccagag gtccagcggt
      781 gagacgggcg accagggtta tccctgccag ggtgacgatc gcatacggca gtgctgccgt
      841 gactttggcg gacagcgtca cgccaagggg cgtaccgtcc gccctcttga cgcggagcca
      901 gcctcgctct ctggcatcgg cgaggccgat acccgggttc gtcaggacca cggacagcac
      961 accaaatgcc ccgtacgtgg ccaacgactc aacgccgaag ccgccgtcgc cgccgaacat
     1021 cgttgcgaac agtgcaaaga atgccactgg catcagcacc gagaagaaga gagcggttgg
     1081 ctccctgacg attcccagaa gctcgtctcg cacctctgtg cgaaagattc ttgtgttcat
     1141 atcagtacct cctcatggtg ggtcatggcg atgaggacct cttcgaggct cgccgtcgcc
     1201 acggtcaaat cgttggcctc ggggtcgagg tcgagcagcg tcctgacgac ctgctcactg
     1261 tcggtcgttg cgatcacggt gcggccgtca cgaagctcca ccgcctccac cgagtcgagg
     1321 gctctgatca cactcaccgg taccttcgtc tggacttcga tccgacggtc tgggagcgtc
     1381 cgacgcagct cgtctggcgt cccttccgcg actactcgtc cgcgatcaac cacaacgacc
     1441 cgatcggcga ctgcggccgc ctcgtccacg agatgcgtcg tgataagcac accggctccg
     1501 ttgtcgagac gccgccgaat cgtgttccag aaccgacgcc gtgcctccac atcgagtcct
     1561 tcggtgggct cgtccagtac cagcagaacc ggatcggcag caagccccat cgcgagctgc
     1621 agccgctgct gctgaccgcc ggagagcttg tccgcccgcc gtttctccag gccggccagg
     1681 cccatctcgg cgatggcttg gctggctgct tccgggggac aaccccggcg ggcggccgca
     1741 ccgccgacga tctcgccgac ggtgagtgac cgcggaaaac cgactgcctg ttggacgaca
     1801 ccgagcctgc gccgggtttc ggcgctacga gggttgccac ctgccacctc gatgaggtcg
     1861 gcgttgcctt cgatcaaccc gatcacattg cggacgaggg tcgtcttacc ggccccattt
     1921 gggccgagaa gtgcgacaca ctcccccgag tcgacagtca gatcgactcc ggcgagagcc
     1981 gtgtagtcac cgtatcggtg aactagacct cgtacttcga gcaaatgttc catctactac
     2041 ctcctttgca ctgatcatgc ggaacgaccg gtgcgacggg tagtgagatc gatcacgaat
     2101 tcgatgtgac agatgtcatc ctgatcaagc cgccttccaa cctcggccct ggcgccaatc
     2161 aggtgggccg tttcagatcc agacgcgaga cttgatgaga cgatgttcgg atgtcccgga
     2221 gcacctcgtc tcctgagagg tgcttcacgg caggaatcct gtgggccgct cgtggagcca
     2281 cggatgagag accgtgctag cggcgaagtt cgaggatcgt cgaatcgccc tgaccagggt
     2341 gttcagtgga gctgggggga cttgaacccc cgaccccctc gttacgaaac atgcggtcaa
     2401 accgctgtga ccaggggata tggcctccaa ttctgggttt gtggagcgtc tgtggggcgg
     2461 acgtcgtgag acatcgtgag acgtggtgag acgtggtgag acgagacagc aaccacctcc
     2521 accccgccag aggcgacgtt gacgcctgat cgaggcacac gttggcgctg ttctcagata
     2581 gcgcatattg ccggttcctg cgtgggtggt tcggtctcat tggtgattgc aggcgcgtgt
     2641 tatgcggtgg cccggcgctt gaacgtcaca gccgaccagg ccgtgactca ataaaccaag
     2701 cctctacgaa acccggggag attcaactcg tgaagtgggc agcgctccgt gaggccttgg
     2761 tcacgaggca aatcgggacc tatggcccta gagccgtcgg acgactctca atagcgtcac
     2821 tcgaaggaga attgaggagc ggctaccaac cacagttggc gatgagccag cttgattcgc
     2881 tgttcgtcaa ctcggtcacg tcggcgaaag aggagatcga tgagattcag gagaatcgcc
     2941 gcacttgtca cggtgatggc gttgtcggtc gctgtcgcca gctgcggtgg cgatgaagcg
     3001 accacatcca cgaccggggc aacggtgaca acggaggcac cggccacgac cgaggcgccg
     3061 gcgaccactg aggcaccggc cacgaccgag gcaccggcga ccaccgcggc accgccagcg
     3121 gagatcttgc ttgacgcacc gctggtgacc gggttcgggg tggatggcga tccaggtgag
     3181 tgggcggatg tgccgtcact cgatctgacg ctggagccga tcgggggcga gacgctcgag
     3241 gccaagaatg cggtcgtgaa ggttgcccat gacggcgaga gtgtctacgt cctgttccag
     3301 gtcgaggacg cctacaactg ggtcctcggc gaggaccccc acttcacctc cgcggtcggc
     3361 gtgcagtggg cgatcgacca gagcgccggt ggcgccatgg gtgccaccga cgacgaccga
     3421 gagacgtccc tgggcacggt cgacatctgg cactgggaac tcgaatgtgc ggccggcgag
     3481 gtctccggcg gcgcgacctc cggacccggt gagggaagtg atcctggaga cgacggggcc
     3541 tgcaacttcg acgacgagtg gtcgaccgat ccggagacgc gcgaggacga caacggtgac
     3601 ggcgccgaga actcgctcag tggttcttgg agtcactcgg atccgacagc tgatgctgct
     3661 ggtacgtgga ccttcgagat gagccgtccc ctcgatacgg gcgattccac cgacgcggcc
     3721 ttcgccgcgg gtgagtcggg tctcctggcc ctcgcctatt gggatgcgga cgccacccct
     3781 gagggttggg aggacgacgg gcatgtccag agtgccaacc tcgggtggat caacgtcacc
     3841 ttcgccagct aggtatagac gggattgacc atcacctggg tggttgccag atcgccgttg
     3901 aggcctccgg caaccacccg gca
//
LOCUS       DATITW010000477         1980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_343490, whole genome shotgun sequence.
ACCESSION   DATITW010000477 DATITW010000000
VERSION     DATITW010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343490"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VLS86_06650"
     CDS             complement(<1..238)
                     /locus_tag="VLS86_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSO50210.1"
                     /translation="MTIGDVAAQAGVSIATVSRVVNGRYGVAPGTSSRVQAVIDDLGY
                     ESSLVARSLRSRRTNVIGIIVADLEPFSAELLKGA"
     gene            complement(354..1865)
                     /locus_tag="VLS86_06655"
     CDS             complement(354..1865)
                     /locus_tag="VLS86_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HSO50211.1"
                     /translation="MTLQASIEIDPAAVIAPVNPRTFGSFVEHMGRCVYTGISEPGHV
                     TADDDGLRGDVLDLVREMGVTVVRYPGGNFVSGYRWEDGVGPVQDRPVRLDPAWKTVE
                     PNTFGLDEFSRWAEKAEVEIMLALNLGTRGVQEALDLIEYCNHPSGTFFSDLRRSHGR
                     EIPYSIRSWCLGNEMDGPWQLGHKTADEYGRLAAETARAMRMIDPDIELVVCGSSGPS
                     MPTFGDWEERVLEHTYEQVDLISAHSYYEPDASDLESFMASAVDMDRFIRTVASIADR
                     VGARKKSRKRIDISFDEWNVWYMRRMSPTPEWDRAPRLAEDLYDVADAVVVGSLLMTL
                     LRNNDRVAVACQAQLVNAISAIRTEPGGPAWRQSIFHPFALTARHAKGEVLSAQPIGP
                     RYDCARYGEVPMVDSVATHDPSTGELSVFSVNRSTEESVQLEIDLRAFPGYQLAGHLM
                     LDDEDHRATNSEHQQNRVEPREAEVQLGVGRADVLLSPVSWSLLRLVHSPRDP"
BASE COUNT          353 a          638 c          623 g          366 t
ORIGIN      
        1 ccgcgccttt cagtagctcg gcgctgaacg gctcgaggtc ggcgacgatg atcccgatca
       61 cgttggtccg ccggctgcgg aggctcctcg ctaccagact cgactcgtaa ccaaggtcgt
      121 cgatcaccgc ctggacccgt gaggatgtac cgggagcaac cccgtatcgc ccgttgacga
      181 cgcgggagac ggtcgcgatg gagacaccgg cctgggctgc cacgtctccg atggtcactc
      241 gcactacagg tcacctcgtc gtcgttgata acgatgtcga gaacgttttc aacactagac
      301 cgaaaccttg ccgtcatgct gcggaaagag aagggatttc ttctggtctc agctcaagga
      361 tctcttggac tgtgcaccag ccgaagcagg ctccaggaaa ccggggacag cagcacatcg
      421 gcccgcccca cgccgagctg gacctccgcc tctctgggct cgacccgatt ctgctggtgc
      481 tcgctgttgg tggcgcgatg gtcctcatcg tccagcatca ggtgaccggc cagttgatat
      541 ccgggaaacg cccggaggtc gatctcgagt tgcacggatt cctccgtgga gcgattgacg
      601 gagaacacac tcagctcccc cgtgctcggg tcgtgggtcg ccaccgagtc caccattggc
      661 acttccccgt atcgggcgca gtcgtaacgg gggcctatgg gctgggcgga gagaacctcc
      721 cccttggcgt gcctcgccgt cagggcgaaa gggtggaata tcgactggcg ccacgccggg
      781 ccgcccggct cggttcggat tgccgagatg gcgttcacga gttgagcctg gcatgccacc
      841 gcgacgcggt cgttgttgcg cagcagcgtc atgaggaggg agcccacgac cactgcgtct
      901 gcgacgtcgt agaggtcctc ggcgagtcgg ggcgcgcgat cccattccgg tgtcgggctc
      961 atccggcgca tgtaccacac gttccactcg tcgaacgaga tgtcgattcg tttacgcgac
     1021 ttctttctcg cccccacccg gtcggcgatc gaggccacgg tgcggatgaa gcgatccatg
     1081 tccaccgcgg aggccatgaa gctctcgaga tcggaggcgt cgggttcgta ataggaatgg
     1141 gccgagatca ggtcgacctg ttcataggtg tgctcgagca cccgttcctc ccagtcgccg
     1201 aaggtgggca tggatgggcc ggagcttccg cacaccacca gttcgatgtc gggatcgatc
     1261 attcgcatcg cacgggccgt ctccgctgcg agacgcccgt actcgtcggc cgtcttgtgg
     1321 ccgagctgcc atggcccgtc catctcgttg cccaggcacc atgagcgaat cgagtatggg
     1381 atctcgcggc cgtgactacg gcgcaggtcg gagaaaaagg tgcctgacgg gtggttgcag
     1441 tattcaatga ggtcaagggc ttcctggacc ccgcgagtgc ccaggttcag tgcaagcatg
     1501 atctccacct ccgccttctc cgcccatcgc gagaactcgt cgaggccaaa agtgttgggc
     1561 tcgactgtct tccaagcggg atcgagccga accgggcgat cttgaaccgg gcccaccccg
     1621 tcctcccagc gatatccgga gacgaagttc ccccccgggt agcggacgac ggtaaccccc
     1681 atctcccgaa ccagatccag gacgtcgccg cggagcccgt cgtcatcggc tgtcacatgg
     1741 cccggctcgc tgatgccggt gtagacacag cgccccatgt gctcgacgaa ggatccgaaa
     1801 gtcctcggat tgaccggtgc aatgaccgcc gcggggtcga tttcaatgct ggcttggagg
     1861 gtcacgatgg agcaagaagc tagcgacgct tccgctccgg ctccacaaag ggcccggttc
     1921 caataacgtt ggcggcgatg accgctctga ccgacaagtt ccggcgcccg gtgcgcgacc
//
LOCUS       DATITW010000478         1867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_423959, whole genome shotgun sequence.
ACCESSION   DATITW010000478 DATITW010000000
VERSION     DATITW010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423959"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..>1867
                     /locus_tag="VLS86_06660"
     CDS             369..>1867
                     /locus_tag="VLS86_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50212.1"
                     /translation="MTSNFQSFAGATPVTVGDFNGDGRPEIALVTCPQTPCSVQIFAV
                     DPSTLAVAIVSSITLPGLTSATTSLTAGRFGATDHDQLVVAYQPASGNAIVATIDFDA
                     SLQPSLKAVAQGITGSAGFFWVKAGRLDWFAPYDYAVLVGGGNIQILSFSRDGNLTPG
                     TQGSGTAIGGCVNDVAVGNFNNTTTNPKPPPPTTIDPDLQIAVLTANECDGGASGGIA
                     VTIYFLFDFQIFVASTFDVPSNALAPSRDCSPSGGPCGWFQNLAVGDLQGRSLALGPP
                     EKVAVTGSIQPHVILGLPPMHIDFIQDTDNVPNGNPTVLNLTAMPSAYFSQYQTEQSH
                     SNQSSNRSTTSYTFSTKESAGAKFSYGVPDVASVSVELKASAQQTHDGSVATTYNTYS
                     SQQFDVSTQTGFSDVVWFTQKRFTTYAYRVLGRCSPATGGCPTGTTKPLYVQFSGPDM
                     VTQGRIDGNQLEWYQPVQEPGNVFSYPWSAGLLQDLYGGFTPLTADPATV"
BASE COUNT          329 a          658 c          564 g          316 t
ORIGIN      
        1 ggagttgccg gcggcggggt gaacaacgcg cagggcaatt tccagaacgg cggtcgggcg
       61 gatgttcagc ctcccaaacg acgtggtggc aaccttgacc ccacccggct tccccacccc
      121 cgctggcgtc aacgtctacg atccggggac gggcacggtg aacttcaccg ccggacccta
      181 cggcggcttc caggtggtcg attccgtgga cgccgccgtc atggccgatt ctaccggcga
      241 cgggtacgct gacctggtga ccctggccac gagcaacgac atgttcgggg gcgccaggat
      301 cgcggtcgcg accgcggcgg atgtggggga acatcggcgc cggcctcact tttggatccc
      361 cgaccgtggt gacctcgaac tttcagtcat tcgccggcgc cacgccggtg acggtcggcg
      421 acttcaatgg cgatgggcga cccgagatcg cgctggttac ctgtcctcag actccatgca
      481 gcgtgcagat cttcgccgtc gatcccagca ccttggccgt cgcgattgtg tccagcatca
      541 ccctgcccgg gctcacgtcg gcgacgacct cgttgacggc gggccgcttc ggcgccacgg
      601 accacgacca actcgtggtc gcgtaccagc ccgcctcggg caacgcgatc gtggccacga
      661 tcgatttcga tgcatcgctg caaccgagcc tcaaggcggt cgcgcagggc attaccgggt
      721 ccgcgggatt cttttgggtc aaggcgggtc gcctagattg gttcgccccc tacgattacg
      781 ccgtgctcgt gggcggtggc aatatccaga tactcagctt ttcgagggat ggcaacctca
      841 cacccggaac acaggggtcg ggcacggcca tcggcgggtg cgtcaatgac gtcgccgtgg
      901 gcaacttcaa caacaccacg acgaacccga agccccctcc gccgaccacc atcgacccag
      961 atctacagat tgcggtgctg accgcgaacg aatgcgatgg cggcgcatcg gggggcatcg
     1021 ccgtgaccat ctactttctc tttgacttcc agatcttcgt agcttcgacg ttcgacgtgc
     1081 cgagcaacgc tctggcgcca tcgcgggatt gcagcccctc cggaggaccg tgcgggtggt
     1141 ttcagaacct ggccgtcggg gaccttcagg gccgctcgct cgccctgggg cctcccgaga
     1201 aggtcgcggt gaccgggagc attcagcccc acgtcatcct gggcctgccg cccatgcata
     1261 tcgacttcat ccaggacacg gacaacgttc ccaacggcaa ccccaccgtc ctcaacctca
     1321 ccgcgatgcc cagcgcgtac ttctcccagt atcaaacgga gcagagccac tccaaccagt
     1381 cctcgaaccg aagcaccacc agctacacct tcagcaccaa ggagtcggcc ggggcgaagt
     1441 tctcctatgg cgtcccggac gtggcctcgg tgagcgtgga gctcaaggcc agcgcccagc
     1501 agacccacga cgggagcgtc gccaccacgt acaacacgta ctccagccag cagttcgacg
     1561 tctccaccca gaccggcttc agcgatgtcg tgtggttcac ccaaaagcgc ttcaccacct
     1621 acgcctaccg ggtcctcggg cggtgcagcc cggcgaccgg gggctgcccg accggcacga
     1681 caaagccgct ctacgtgcag ttctccggcc ccgacatggt cacgcagggc cgcatcgacg
     1741 gcaaccagct cgagtggtac cagccggtgc aggagcccgg caatgtcttt tcgtatccct
     1801 ggagcgccgg cctccttcag gatttgtacg gggggtttac ccccctgacc gccgatccgg
     1861 ccaccgt
//
LOCUS       DATITW010000479         1778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_263591, whole genome shotgun sequence.
ACCESSION   DATITW010000479 DATITW010000000
VERSION     DATITW010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263591"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VLS86_06665"
     CDS             complement(<1..529)
                     /locus_tag="VLS86_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005162700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50213.1"
                     /translation="MRYGSNDVLNGVDFDVHRGEVVTLLGPNGAGKTTTIEILEGFRI
                     ASDGQIRVLGVDPSRGDEQWRARVGVVLQSWRDHARWTPRDLLAHLGRYYVPHSTPER
                     IRPYDIEELISMVGIEEQAEQKIRTLSGGQRRRIELARILFAAAETMILDEHTNHLDA
                     DSVVWLREFVKNYRGG"
     gene            697..1104
                     /locus_tag="VLS86_06670"
     CDS             697..1104
                     /locus_tag="VLS86_06670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSO50214.1"
                     /translation="MISYRLGNYLDLDQVIDLYVDSTLGERRPVGERERMRTMLDEAN
                     LVVTAWDGDLMVGISRSVTDRVYCTYLSDLAVRLGYQSQGIGRELVRRTRQETPRANL
                     ILLAAPKAVDYYPKIGMTRHDSAWILPPGVEPV"
     gene            complement(1117..>1778)
                     /locus_tag="VLS86_06675"
     CDS             complement(1117..>1778)
                     /locus_tag="VLS86_06675"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO50215.1"
                     /translation="NGPFPAVVLVHGGGWVAGSPSIMRPLAQHLTDAGYLTVNTQYAL
                     SGEQPGFPAAVDDVTCAVRYAAAHPDSDGTVAVLGHSAGAHISAVVALSGDDYGDSCP
                     IAGTGLPDRLVGLAGPYDVARLGILMIPFFGVGPAEDPEAWAAGNPIHLVDQNPALDG
                     LLMYGEEDGLIDPSFTFDFAEALEAEGSEALVEGVEDAHHNDMYAPRFVGDLIATWLD
                     G"
BASE COUNT          325 a          571 c          565 g          317 t
ORIGIN      
        1 agccaccgcg gtagttcttc acgaactcgc gcagccacac gaccgagtcg gcgtcgaggt
       61 ggttggtgtg ctcatcgagg atcatggtct cggcggcggc gaagaggatg cgggccagtt
      121 cgatacggcg gcgctggcca ccggagaggg tgcggatctt ctgctccgct tgctcctcga
      181 tccccaccat ggagatcaac tcctcgatgt cataggggcg gatgcgctcc ggggtggagt
      241 ggggcacgta gtacctgccg agatgggcca gaaggtcgcg cggagtccag cgggcatggt
      301 cacgccatga ctggagcacc actcctacac gtgctcgcca ctgttcgtct ccgcgggacg
      361 ggtcgacccc aagaaccctt atctggccgt cggaggcgat cctgaagcct tcgaggatct
      421 cgatcgtggt ggtcttgccc gctccgttgg gcccaagcaa agtgacgacc tccccgcggt
      481 gcacgtcgaa gtcaactcca ttgaggacat cgttagatcc gtagcgcatc cgcaggcccc
      541 ggacctcaac tacccggtca cggttggtcg caccgattcc tgtaacaaat ctcttctctg
      601 aggtatcaga actcatgcga tcggtagcat aaatactacg agttgatctt ggcaagggat
      661 ttctcggcca agtggtgcgg tctaccctcc atccccgtga tcagctaccg attggggaac
      721 tacctcgacc tcgaccaggt catcgatctc tacgtggact cgacgctcgg agagcgccga
      781 ccggttggcg agcgagagcg gatgaggacc atgctggatg aggcaaatct ggtggtcact
      841 gcctgggacg gggatctgat ggtcggcatc tcccgttcgg tcaccgatcg ggtctactgc
      901 acctatctct cggatttggc ggtacgactc ggctaccagt cccaggggat cggccgggag
      961 ctggtgcgga gaacccgtca ggagacccct cgcgccaacc tcatcctgct cgccgccccc
     1021 aaggccgtcg actactaccc caagatcgga atgacccggc acgacagtgc ctggattctc
     1081 cccccgggcg tcgagccggt ctgagaccgg agacgctcac ccgtccagcc aggtggcgat
     1141 caggtcaccg acgaaacgag gggcatacat gtcgttgtga tgggcgtctt cgactccctc
     1201 cacgagtgcc tccgaccctt cagcttcgag ggcttcggcg aagtcgaacg tgaacgaggg
     1261 gtcgatgagg ccgtcctctt cgccatacat caggagaccg tcgagcgctg ggttctgatc
     1321 caccaggtgg atcgggttcc cggctgccca tgcctcggga tcctcggcgg gaccgacgcc
     1381 gaagaagggg atcatgagaa ttccgaggcg ggcgacgtcg tagggccctg ccaacccgac
     1441 gagacgatcc gggagcccgg taccggcgat cgggcacgag tcgccgtagt cgtcgccact
     1501 caaggccacc accgccgaga tgtgggcccc tgcggaatgc cccagcaccg ccaccgtgcc
     1561 atcggagtcc ggatgcgccg ccgcatagcg aaccgcacag gtgacgtcgt ccacggccgc
     1621 cgggaagccc ggctgttcgc ctgacaacgc gtattgggtg ttgaccgtga gataaccggc
     1681 atcggtcagg tgctgggcga gcggtctcat gatcgaaggg ctccccgcca cccagcctcc
     1741 gccgtggacc aggacgacag ccgggaatgg cccgttcc
//
LOCUS       DATITW010000480         3959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_183267, whole genome shotgun sequence.
ACCESSION   DATITW010000480 DATITW010000000
VERSION     DATITW010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183267"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VLS86_06680"
     CDS             <1..203
                     /locus_tag="VLS86_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HSO50216.1"
                     /translation="QAQGILDAMDRIVDLLAEMDYPDGMTGGLRRTTDVSRSLVERSR
                     SDLTATVVQERLRHDLSKLLQE"
     gene            258..331
                     /locus_tag="VLS86_06685"
     tRNA            258..331
                     /locus_tag="VLS86_06685"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:292..294,aa:Ala,seq:cgc)
     gene            complement(597..1592)
                     /locus_tag="VLS86_06690"
     CDS             complement(597..1592)
                     /locus_tag="VLS86_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSO50217.1"
                     /translation="MTNGPRPAPGQRSGWLTRVLLAFVTSSSILATASPADAAEIEVE
                     APAESTVGEVVQVSATITDGGQPVAEAVVALTRQAELAGTSGFVELDRGVTDEAGMVA
                     FGFVQYADSSEVAAMRVEYQGPDGIEAAEFELTVLPGSQQFTSSSGADIGIFNVSWLI
                     VVIGLVWVFLIVATWQVQVISHARGGSKGGSRALPYLMVGFVAFTALGMFYVVLTQPT
                     MHANLAPNEPFDRAVTAYVGEEHEYTGLGLHGRRSDLSGEALYVQAGCVSCHGIDGRG
                     GVVGGELSVSTMSDTDEVIEAIRRGPMGMPVYSEQALSDEEIDRIIDYLTAASGG"
     gene            complement(1589..2926)
                     /gene="nrfD"
                     /locus_tag="VLS86_06695"
     CDS             complement(1589..2926)
                     /gene="nrfD"
                     /locus_tag="VLS86_06695"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HSO50218.1"
                     /translation="MDPLTEGSNTGRGSWDDRLDEAVLSPARRSGIGFRLWVGLLIVL
                     IAVGVIAVINQSRNGLYVTGMRDRISWGLYIAAFVFFAGISMAGTLISAILRASHATW
                     RTPITRIAESITVMALVVSALFVLVDMGKPVRLFQMWPNGNWQSPLMWDMMAITVYLT
                     CSVVYLLVPMIPDLAFFQDRLRGRVPRLHSRVYETLSFGWEGNPVQRRALAKSVAILM
                     VLIIPVAVSVHTVLSLVFSTTTRVSWNSTAFGFFFVFGAIFSGLAVLIIIFAVVRKRF
                     HLEEYITERHFTYLGYLLATAGMVMIYANATEFITKGYHASADDLFAFRELFVTDLAP
                     LFWFYFFGGLVLPVLIVAFKRTRTITGLVIGSSFAIVAMFVERYFIVVGGMRVPLMPY
                     EPSSYAPTWVEWALIVGGMALFVLLLTLFTRFFPILAVWEMKEEHAERSGVTS"
     gene            complement(2914..3726)
                     /locus_tag="VLS86_06700"
     CDS             complement(2914..3726)
                     /locus_tag="VLS86_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSO50219.1"
                     /translation="MLGLSTMTPSEAATSQTPASGPGGRVRQWGMVIDLRRCDGCQSQ
                     STPPKCTLACIEGHLAPEPMEWIEVYETELAGGGTQFLPTPCLQCQNPPCVNVCPVAA
                     TYSTPDGVVQTDQNRCIGCRMCMAACPYDRRFFNWGTAPIPPEAFLADYAADHQTPAQ
                     RGTVMKCDFCVDMVRGGSLPMCVQACPQGAIYYGDMEEDIATNGAALIGFGDLLANHN
                     AYRLKEELGTQPRVFYIPGHGEDVGRDRHTEGRLPTIWPWIERAKGAVTWTR"
BASE COUNT          806 a         1262 c         1235 g          656 t
ORIGIN      
        1 cccaggcgca gggcatcctc gacgccatgg accggatcgt cgacctgctc gccgagatgg
       61 actaccccga cggcatgacc ggcgggctcc gccgcaccac cgacgtctcc cgctcgctgg
      121 tggagcgttc ccgatccgat ctcaccgcca ccgtcgtcca ggagcgtctc cgccacgacc
      181 tctccaaact gctgcaggag tagctgacac cgatctggcc caccatctgc ccgtcggtac
      241 aatccaccgt cccgcaaggg gctgtagctc agcctggcag agcacctcgt tcgcaacgag
      301 ggggtcacgg gttcaaatcc cgtcagctcc actgatttcg ccctggtcag agggggttct
      361 tctcgttttg cgggaagttc ctcttcgacc aacgcctgag gagagctccc gccgccatgc
      421 aggcgactgt gaataatcag gggaggcgga gcaacacgaa taaagagctg gaccgaggga
      481 ccgtctcccc caaccgctgc tcggcgatct gtgacggacc catgccggcc aggcgaacgg
      541 ctccccaacc ggtcgcggct tgtgcgccca gattgtccct gagcaaccgg cgtggttcaa
      601 cctccgcttg cggcggtcag gtaatcgatg atcctgtcga tctcctcatc tgacaaggct
      661 tgctcgctgt acaccggcat ccccattggg ccccgccgaa tggcttcgat cacttcgtcg
      721 gtgtccgaca tggtggagac gctgagctca ccacccacaa ccccaccgcg gccatcgatt
      781 ccatgacacg acacacaccc ggcttggaca tagagcgctt caccactgag atcgctgcgc
      841 cgcccgtgca agccaagccc ggtgtactcg tgctcctccc caacgtaggc ggtaacggcg
      901 cgatcgaagg gctcgttggg ggcgaggttg gcatgcatcg ttggctgggt gaggaccacg
      961 tagaacatac cgagggcggt aaacgcgacg aacccgacca tcaggtaggg caaagcgcgg
     1021 cttcccccct tcgaaccgcc tcgggcatgg ctgatgacct ggacctgcca ggtcgccacg
     1081 atgagaaaga cccacaccaa accgatcacg acgatgagcc agctgacatt gaagatgccg
     1141 atgtccgcac cggaggagct cgtgaattgc tgtgacccgg gcagcacggt gagctcgaac
     1201 tcggcggcct cgattccgtc cggaccctgg tactcgactc gcatcgccgc gacttcggaa
     1261 ctgtccgcat actggacgaa cccgaaggcg accatgccgg cctcgtcggt gaccccccga
     1321 tcgagctcga cgaaacctga cgttccagcc agctcggcct gccgggtgag ggcgacgacg
     1381 gcttcagcaa cgggctgacc gccgtcggtg atggttgcgg aaacctggac gacctcaccg
     1441 acagtcgact cggcgggagc ttcgacctcg atctcggcag catccgcagg gctggcggtc
     1501 gccagaatcg agcttgaggt gacgaaggca aggaggacgc gggtgagcca tccactcctc
     1561 tgacctggag cgggccgcgg tccgttcgtc atgaggtgac ccccgagcgc tcagcgtgct
     1621 cctccttcat ctcccagacc gcgaggatcg ggaagaacct ggtgaacaaa gtcagcagga
     1681 gcacgaacag ggccatcccg ccgacgatca gcgcccactc gacccaggtc ggcgcgtagc
     1741 tcgagggctc gtagggcatc agaggaaccc tcatcccgcc gacgacgatg aagtaccgct
     1801 cgacgaacat cgcgacgatc gcaaaactcg acccgattac caacccggtg atggtgcgtg
     1861 tgcgcttgaa agcgacaatc agcaccggga gtacgagccc accgaagaag tagaaccaga
     1921 acagaggtgc cagatcggtc acgaacagct cccggaaggc gaacaggtcg tcggcgctcg
     1981 cgtgatagcc cttggttatg aactcggtcg cattggcgta gatcatcacc atgccggcgg
     2041 tggcaagcag gtaaccgagg taagtgaaat gacgctctgt gatgtactcc tcgagatgga
     2101 atcgcttcct caccacggcg aagatgatga tcaggacagc gaggcccgag aagatcgccc
     2161 caaagacgaa gaagaacccg aaggcggtgc tgttccagct gacgcgagtc gtagtggaaa
     2221 agaccaacga caggacggtg tgaaccgaca cagcgaccgg gatgatcaac accatcaaga
     2281 tcgccacgga cttggccagc gcccgccttt ggacggggtt cccttcccat ccgaacgaca
     2341 gcgtctcgta caccctcgag tggagccgtg ggacgcgccc tctgagtcgg tcctggaaga
     2401 aggcgaggtc agggatcatc ggcaccagga ggtacaccac actgcaggtg agatagaccg
     2461 tgatcgccat catgtcccac atgagcggtg actgccagtt gccattaggc cacatctgga
     2521 agagtcgtac tggcttgccc atgtcgacca ggacgaacaa cgcagagacc accagagcca
     2581 tgaccgtgat cgactcggcg atccgggtga tcggtgtgcg ccaggtggca tgagaggccc
     2641 gcaggatcgc cgatatcaag gtccctgcca tgctgatgcc cgcaaagaag acaaaagctg
     2701 cgatatagag accccatgag atgcgatcgc gcatcccggt cacgtagaga ccgttccggg
     2761 actggttgat gacggcgatg acgcccacgg cgatgaggac gatcagcaaa cccacccaga
     2821 gcctgaagcc gattcccgac cgccttgcgg gggagaggac cgcttcgtcg agtcgatcgt
     2881 cccatgagcc ccggccggta ttcgatccct cggtcagcgg gtccatgtca cggctccctt
     2941 ggcacgctca atccacggcc agatcgtggg caaacgacct tcggtgtgtc gatctcgtcc
     3001 gacatcctcg ccgtggccag ggatgtagaa aacgcggggc tgagtgccga gctcctcctt
     3061 caggcggtaa gcgttgtggt tcgccagaag gtccccgaac ccaatcaagg cggcaccgtt
     3121 ggtggcgatg tcctcctcca tgtcgccgta gtagatggcc ccctggggac aggcctgaac
     3181 gcacatcggc agggacccgc ctcgtaccat gtcgacgcag aagtcgcact tcatcacggt
     3241 tcccctttgg gccggggtct gatggtcagc tgcgtagtcg gctaaaaagg cctcgggtgg
     3301 gattggggcg gttccccagt taaagaagcg gcgatcgtac gggcaggccg ccatgcacat
     3361 acggcagccg atgcagcggt tctggtcggt ctggaccaca ccgtcgggtg tggagtaggt
     3421 cgctgcaacc gggcaaacgt tgacacaggg cgggttctgg cattgcagac acggggttgg
     3481 gaggaactgt gtgccaccgc ctgccaactc agtttcgtag acctcgatcc actccatggg
     3541 ctccggcgcg aggtgccctt cgatgcaagc gagtgtgcat ttaggcgggg tcgactggct
     3601 ctgacatcca tcgcaacgtc gaagatcgat gaccatcccc cactgccgaa cccgaccgcc
     3661 tggcccggac gctggggtct ggctggtcgc tgcctcgctg ggcgtcatgg tcgacaagcc
     3721 gagcattggt gctgccaatg cgacgccgcc cgcaccgagg agcttcaggg cccttcgccg
     3781 agtgaaggcg atcggctcgg cggaaggcgc cgacgggccc gatgccgggt gccccaaggg
     3841 cgccgggcgg ctttcgacgg ctgggtttcc tcgaatctgg ccgcggagag tgcgccgatg
     3901 caggtacgtg tagacgatgg caccggtcaa tccgataacg agcagagctg cagccaggg
//
LOCUS       DATITW010000481         3823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_784209, whole genome shotgun sequence.
ACCESSION   DATITW010000481 DATITW010000000
VERSION     DATITW010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784209"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VLS86_06705"
     CDS             complement(<1..537)
                     /locus_tag="VLS86_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HSO50220.1"
                     /translation="MAKVIGIDLGTTNSVVAVVEGGDPAVIANQEGSRLTPSVVAFTK
                     EGDTLVGQVAKRQAITNPENTIFSIKRFMGRRYDEVLSEAKLIPYKVLKADNGDVRVE
                     SRGKVYSPPEISALVLRKLKEAAEAYLGEKVTQAVITVPAYFNDSQRQATKDAGAIAG
                     LEVLRIVNEPTAAALAYGL"
     gene            complement(637..1101)
                     /locus_tag="VLS86_06710"
     CDS             complement(637..1101)
                     /locus_tag="VLS86_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSO50221.1"
                     /translation="MALIRLRPFTQVLDPSGDVMGMQTQMNRLFDNFFGQPSQPGMME
                     RAWTPPVDMYETKNEVVVTVELPGVNDKDIRLSITGDLLTIQGERQWSDETRDAGHYR
                     QERWFGKFERALSLPIPVETGQVKATYRDGVLTVKLPKTEGVKPKEIKIDAA"
     gene            1380..2192
                     /gene="mazG"
                     /locus_tag="VLS86_06715"
     CDS             1380..2192
                     /gene="mazG"
                     /locus_tag="VLS86_06715"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HSO50222.1"
                     /translation="MADAAGTRFVSLLSLMARLRAEGGCPWDREQTRTSLRPYVIEEA
                     YEVVHAIDEGSQDHLVEELGDLLFQVVFHSQIGAEAGEFTMDDVLTRVCDKMTRRHPH
                     VFGGGAVADSQEALAQWERIKREETGSTGPTLDGVPASLPALLRAQKLQGKAARVGFD
                     WPNWQDAWSKVGEEMGEVQEALTGGDDARVADELGDLLFSIVNVARLRGMDAEDCLRR
                     ASEKFTRRFGKVETEMKAGGRTVTEASAADLDRAWEAVKAQEDQRPEPRVRS"
     gene            2189..2725
                     /locus_tag="VLS86_06720"
     CDS             2189..2725
                     /locus_tag="VLS86_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HSO50223.1"
                     /translation="MKCKIGKTTVIVERGDITEAEVDAVVNAANAELWMGAGVAGAIK
                     RKGGVVIEEDAIRQGPVEIGEAVLTVAGNLAATHVIHAVTMGKDLKVDPDNITAATRA
                     TLAIAEKHKLSSLAFPALGSGVGGVPAAQSADAMLTTVVEHVKRGSSSLEKVLFVLYE
                     DQAQKAFSDVLKRLGGVR"
     gene            2985..>3823
                     /locus_tag="VLS86_06725"
     CDS             2985..>3823
                     /locus_tag="VLS86_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HSO50224.1"
                     /translation="MAATMHDLLTLMIERGASDLHVTTGTHPQIRLNGKLTPISQFEP
                     LSPTDTQRLAYSVLNEGQKQKFEEDNELDLSFGIHGLARFRCNVYRQRGAVACAIRVI
                     PVKIRTFDELGLPAIVEQMADRPKGLILVTGPTGSGKSTTLAAMVDKVNSERSEHIVT
                     IEDPIEFVHQHKKCMVNQREVFSDTHSFKNALKSILRQDPDVVLVGEMRDLETISAAL
                     TIAETGHLTLGTLHTNSCAQTINRIIDVFPTSQQAQVRAQLSLVLEGVLSQQLIPTAD
                     GRGR"
BASE COUNT          662 a         1243 c         1252 g          666 t
ORIGIN      
        1 gagcccgtag gcgagcgccg ccgcggtggg ctcgttgacg atgcggagca cctcgagccc
       61 ggcgatcgcc cccgcgtcct tggtggcctg ccgctggctg tcgttgaagt aggccgggac
      121 cgtgatcacg gcctgggtga ccttctcccc caggtaggcc tcggccgcct ccttgagctt
      181 gcgcagcacg agggcggaga tctccggagg ggaatacacc ttgccgcggc tctccacgcg
      241 cacgtccccg ttgtcggcct tcagcacctt gtacgggatg agcttggcct cactgagcac
      301 ctcgtcgtag cgacgcccca tgaagcgctt gatggagaag atggtgttct ccgggttggt
      361 gatggcctgg cgcttcgcca cctgcccgac gagggtgtcg ccctccttcg tgaatgccac
      421 taccgacgga gtcaggcggc tcccttcctg attggcgatg acggcggggt caccgccttc
      481 caccaccgcg acaaccgagt tcgtcgttcc gaggtcgata ccgatcacct tcgccatgtc
      541 gtgttctctc ctgtctgagt gtcccggggc tgcaccgggc cggcaggccg gacgtgatcg
      601 ccccggccta ccggcccccg gcggattccg cggaaattag gccgcgtcga tcttgatctc
      661 cttgggcttc acgccttcgg tcttcggaag cttcaccgtg agcacgccgt cccgataggt
      721 cgccttgacc tgcccggtct cgacgggaat cggaagcgac agcgcccgct cgaacttccc
      781 gaaccagcgc tcctgacgat agtggcccgc gtcgcgcgtc tcgtcgctcc actgacgctc
      841 gccctggatc gtgagcagat cgcccgtgat ggagagccgg atgtccttgt cgttgacgcc
      901 gggcagctcc accgtgacga cgacctcatt cttggtctcg tacatgtcga ccggcggagt
      961 ccacgctcgc tccatcattc ctggctgcga gggctgtccg aagaagttgt cgaagaggcg
     1021 gttcatctga gtctgcatcc ccatcacgtc tccggatgga tccagcacct gggtaaaggg
     1081 acggagtcga atgagcgcca tcgttgctcc tcctgtctgc ggggcgcctt gccccgtctt
     1141 gatatgagcg tacttgactt atatatgctg agttgatcga tgtcaaatag ctcggaatta
     1201 ttcaatatat ggaagcctta ttaggcactt acatggatcg cgtagtccgg cgaaggcatc
     1261 tagccattcc ggagagcact ccgggggcac ccggtagggg acgacgggga tatgcgtgcg
     1321 ggcaccgcct cgcccgcgcc tgttgtgtat aatgccggtg cgactcgacg attgtcccaa
     1381 tggctgacgc cgccggcact cgcttcgtct ccctcctctc cctcatggct cgcctcaggg
     1441 ccgaaggtgg ttgcccgtgg gatcgcgagc agacccggac ctctcttcgt ccgtatgtca
     1501 tcgaggaggc atacgaggtc gtccacgcca tcgatgaggg ctcgcaggat cacctcgtgg
     1561 aggagctcgg ggatctgctg ttccaggtcg tcttccacag tcagatcgga gccgaggccg
     1621 gggagttcac gatggatgac gtcctgacga gggtctgcga caagatgacc cgtcggcacc
     1681 cgcacgtatt cggaggcggc gcggtggccg attcccagga ggccctcgcc cagtgggagc
     1741 gcatcaagcg ggaggagacc ggctcgacgg gccccaccct cgacggggtt cccgcgtcgc
     1801 ttcccgccct gctccgggcg cagaagctcc agggcaaggc ggcccgcgtc ggcttcgact
     1861 ggccgaactg gcaggacgcg tggagcaagg tgggggagga aatgggcgaa gtccaggagg
     1921 cgctgaccgg cggagacgac gcccgcgtcg cggacgagct cggtgatctg ctgttttcga
     1981 tcgtcaatgt ggcgcggcta cggggcatgg atgccgagga ctgcctgcgc cgagcctccg
     2041 agaagttcac ccgacgcttc gggaaggtgg agacggagat gaaggcgggc ggccggacgg
     2101 tcactgaggc atctgcggcg gacttggatc gagcctggga agcggtcaag gcgcaagagg
     2161 accagagacc cgagccgagg gtgcggtcat gaagtgcaag atcggcaaga cgaccgtcat
     2221 cgtcgagcgg ggagacatca ccgaggccga ggtcgacgcg gtggtcaacg ccgccaacgc
     2281 cgagttgtgg atgggcgccg gcgtcgccgg cgctatcaag cggaagggcg gagtggtcat
     2341 cgaggaggac gccatccggc agggacccgt ggagatcggg gaggcggtgc tgacggtcgc
     2401 cgggaacctg gcggcgaccc acgtcattca cgcggtcacc atgggcaagg atctcaaggt
     2461 cgaccccgac aacatcaccg cggcgacccg ggccaccctg gccatcgcgg agaagcacaa
     2521 gctctcctcg ctcgcattcc cagccctggg gagtggggta ggcggggtcc cggcggccca
     2581 gtccgccgac gcgatgctga ccacggtggt cgagcacgtc aagcgcggca gctcgagcct
     2641 ggagaaggtg ctcttcgtgc tctacgagga ccaggcccag aaggccttca gcgacgttct
     2701 caagcgcctc ggaggcgtac gctagccctc atttcgcgca ctggagctac atccagtgtg
     2761 ggatcccggc cttgaagtcg cccggccctc tcccgggccc tcagccaata gggggttgct
     2821 aaaaccaccc tgggctagaa tggcttgtcg ccactggtga ccggccccgg tcgggcatcc
     2881 gtcgcgtccg caggttaccc tggcaatctg gaatgcaggt tgcatgcgcc ttgaggggcc
     2941 ctggggcata cactcaggtc ttatagggag gttcaaggga accaatggcg gccacgatgc
     3001 acgacctgct gaccctgatg atcgagcgtg gggcatccga cctccacgtc acgacgggca
     3061 cccacccgca gatccggctc aacggcaagc tcacgccgat cagccagttc gagcccttga
     3121 gcccgaccga tacccagcgg ctggcctaca gcgtcctcaa cgaaggccag aagcagaagt
     3181 tcgaggaaga caacgagctt gacctgtcgt tcggcattca cggtctggct cgcttccggt
     3241 gcaacgtcta ccgccagcgt ggcgcagtgg cctgcgccat tcgcgtcatc ccggtgaaga
     3301 tccggacctt cgacgagctg gggctccccg cgattgtcga gcagatggcc gaccggccca
     3361 agggcctgat cctggtcacc ggccccaccg gctccggaaa gtccacgacc ctggccgcga
     3421 tggtggacaa ggtcaactcc gagcgctcgg agcacatcgt caccatcgag gacccgatcg
     3481 agttcgtgca ccagcacaag aagtgcatgg tgaaccagcg cgaggtgttc tcggacactc
     3541 actcgttcaa gaacgcgctg aagtcgatcc tgcggcagga ccccgacgtc gtgctcgtcg
     3601 gcgagatgcg ggatctcgag accatctcgg cggccctgac catcgcggag accggccacc
     3661 tgacgctcgg tacgctccac accaactcct gcgctcagac catcaaccgg atcatcgacg
     3721 tcttcccgac cagccagcag gcgcaggtgc gcgcccagct ctctctggtg ctcgaaggcg
     3781 tactctcgca gcagttgatc ccgaccgcgg acggccgggg ccg
//
LOCUS       DATITW010000482         1586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_103895, whole genome shotgun sequence.
ACCESSION   DATITW010000482 DATITW010000000
VERSION     DATITW010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103895"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..840
                     /locus_tag="VLS86_06730"
     CDS             169..840
                     /locus_tag="VLS86_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HSO50225.1"
                     /translation="MERAQRAAVHRALGDEARLRMVYELALSDRTPQELARITGSETN
                     LLAHHLGVLAEAGLVRRRVSSGDRRRRYISLETGRVRGLLPELRLRASSVVFVCTHNS
                     ARSQFAEALLRSRSKIRVQSAGPNPRPSLHPKAIQVGTELGVDLRGHRPKGYADVEGS
                     PDLVISVCDLALEARIPFQARRLHWSIPDPVESGRVEDFRRSFTEIERRVGLFLDAIE
                     REEQP"
     gene            837..1490
                     /locus_tag="VLS86_06735"
     CDS             837..1490
                     /locus_tag="VLS86_06735"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HSO50226.1"
                     /translation="MTTTRASLSTAEQLHLRQGAERLKRRFEGELNTETIERFLYDSL
                     DRLLGKAQTTAWVPLLAERFAADRIRAMIRLESDVTTLNPSVLFLCVHNAGRSQMAAG
                     LMRKLAGEQVDVFTGGSEPAATVNQAAVAAMAELGIDISAEIPQPWADEIVRAADVVV
                     TMGCGDACPIYPGKKYVDWEVDDPSGKTVDEVRPIRDELEQRVRTLMTELGVSPIGG"
BASE COUNT          285 a          470 c          544 g          287 t
ORIGIN      
        1 gtggcccttt ccggccgctc cgatgagtta cggagctggc tgggcactga tgacgggttg
       61 tccattgtcg acggtccgcc gggaatcgag agcgtgacca tcgagatgcg atctggtccg
      121 attgaactga attgactcag ccaagattga gtcaatatag tgtgagctat ggagagagct
      181 cagagggcgg cagtccacag ggcgctggga gacgaagccc ggcttcggat ggtctatgag
      241 ctggccctct ccgacaggac accgcaggag ctggcccgga tcaccggctc cgagaccaat
      301 ctgctcgccc atcacctcgg agtgttggcg gaggcgggac tggtccggcg ccgggtgtcg
      361 tccggtgatc ggcggcgtcg ctacatctca ctcgagaccg ggagggtgcg cgggctcctc
      421 ccagagctcc ggctgagggc aagctcggtg gtgttcgtgt gcactcacaa ctccgcccgc
      481 tcccagttcg ccgaggccct gcttcgctcg agatccaaga tccgggtgca aagcgcgggc
      541 cccaatccca ggccgtcgct gcaccccaag gcgatccagg tggggaccga gttgggggtg
      601 gatctccgtg gtcatcggcc caaggggtat gccgacgtcg aagggtcacc ggacctggtg
      661 atctcggtat gtgatctggc gctggaggcg aggatcccct tccaggcccg gcgcctgcac
      721 tggtcgatcc cggatccggt ggagagcggc cgggtggagg acttccgcag gtcgttcacc
      781 gagatcgaac gacgtgtcgg cttgtttcta gatgccatcg aacgagagga acagccatga
      841 ccaccaccag ggccagtctg agcacagcgg agcagcttca tctgcgtcag ggggcggagc
      901 gtctcaagcg gcggttcgag ggtgagttga acaccgagac catcgaacga ttcctctacg
      961 actcgctgga ccgactgctc ggtaaggcgc agacgacggc ctgggtccca ctgctcgccg
     1021 agcggttcgc cgcggatcga atccgggcaa tgatccgtct agagtccgac gtcaccaccc
     1081 tcaatccaag tgtgttgttc ctctgcgtcc acaacgcagg ccgctcccag atggcggcgg
     1141 ggctgatgcg gaagctggcg ggggagcagg tcgatgtctt caccgggggc tccgagcctg
     1201 ccgccacggt gaaccaggcg gcagtggccg ccatggccga gttgggcatc gacatcagcg
     1261 ccgagatccc ccagccctgg gccgacgaga tcgtccgtgc cgccgacgtg gtggtgacca
     1321 tgggctgtgg cgacgcctgt cccatctacc cagggaagaa gtacgtcgac tgggaagtcg
     1381 acgatccttc aggaaagacg gtggacgagg tgcgcccgat ccgtgacgag ttggagcagc
     1441 gggtgcggac gctcatgacc gagctcgggg tgtcgccgat cgggggctga ccggtcatag
     1501 atcttgaccg ctgtcatatc atcttgatat catcgtgata tgacagaaag gcaatcgatg
     1561 tccgctgagc ggttgatcgt cggttt
//
LOCUS       DATITW010000483         1536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_704562, whole genome shotgun sequence.
ACCESSION   DATITW010000483 DATITW010000000
VERSION     DATITW010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704562"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VLS86_06740"
     CDS             <1..535
                     /locus_tag="VLS86_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50227.1"
                     /translation="SEAVVVSSLEKIGIKVKPRQLASSAAYTTIQTVKNMIPIALNAG
                     WGKDYADPYSFAGALFDSRSIIATGNTNYPLLGMTSEQAKSLGIEIPSGVTIPSVDAD
                     IDACQEIALDKPDDRNQCFADLDKKMMEESLPWVPYLWAKNVTITGTTVTKWEFDQFS
                     GYLSYTQMAVNNGETIS"
     gene            633..>1536
                     /locus_tag="VLS86_06745"
     CDS             633..>1536
                     /locus_tag="VLS86_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO50228.1"
                     /translation="MLWVVVVLLVVTSITYVVFFVMPSTDPAVTFAGKNPTPEQIEEV
                     RKQFGLDKPVPVQYGIFIKNVFLGDQYGWPGLGFSYSTRSPVKDQFFGRILVTVQLAF
                     GAALIWLAIGIPIGIISAIRSRTFTDRAAMGFALFFVAAPVFWLGLMGLWLFWFKLRW
                     SPGTGYVAWGDSFGDWLSHMILPWVVLAMLYAAFYARMVRGNLIETMGQDYIRTARVK
                     GLSERTVIFKHGLRASLTPIVTPIGLDLGALLGGAVITETVFNLQGVGQWAVTSVFQG
                     DLPVVLAVTVVVAIAVTLMNLIVDI"
BASE COUNT          250 a          524 c          495 g          267 t
ORIGIN      
        1 ctccgaggcg gtcgtcgtct cgagcctgga gaagatcggc atcaaggtga agccgcgcca
       61 gctcgcctcc agcgccgcct acaccaccat ccagaccgtc aagaacatga tcccgatcgc
      121 cttgaacgcc ggctggggca aggactacgc cgacccctac agcttcgcgg gggcgctgtt
      181 cgactcccgc tccatcatcg cgaccggcaa caccaactac ccgctgctgg ggatgacctc
      241 cgagcaggcc aagagcctgg ggatcgagat cccgtccggg gtcaccatcc cgagcgtcga
      301 cgccgacatc gacgcctgcc aggagatcgc cctcgacaag ccggacgacc gcaaccagtg
      361 cttcgccgac ctggacaaga agatgatgga ggagtcgctc ccctgggtcc cgtacctgtg
      421 ggccaagaac gtcaccatca cggggacgac cgtgaccaag tgggagttcg accagttctc
      481 cggctacctg tcctacacac agatggcggt gaacaacggg gagaccatct cctgacagac
      541 agatgagggg cgacgggggt cgccggaccg gcggcccccg cccctcctgg cgaacggctg
      601 ggtgaaccat gtggcggtac atcgtccgac ggttgctgtg ggtcgtggtc gtgctgctgg
      661 tcgtgaccag tatcacctac gtcgtcttct tcgtgatgcc ctccaccgac ccggccgtga
      721 ccttcgccgg caagaacccg acccccgagc agatcgagga ggtccgcaag cagttcggcc
      781 tggacaagcc ggtcccggtc cagtacggca tcttcatcaa gaacgtcttc ctgggtgacc
      841 agtacggctg gccgggcctc gggttctcct actcgacccg ctcgccggtc aaggaccagt
      901 tcttcggccg gatcctcgtg acagtccagc tggccttcgg ggccgccctg atctggctgg
      961 ccatcggcat ccccatcggg atcatctcgg cgatcaggag ccggaccttc accgaccggg
     1021 ccgcgatggg gttcgcgctg ttcttcgtgg ccgccccggt gttctggctg gggctgatgg
     1081 gcctgtggct gttctggttc aagctgcgct ggtcccccgg caccggctat gtcgcctggg
     1141 gcgacagctt cggcgactgg ctcagccaca tgatcctgcc ctgggtcgtg ctggccatgc
     1201 tgtacgcagc cttctacgcc cgcatggtcc gcggcaacct gatcgagacc atgggccagg
     1261 actacatccg cacggccagg gtcaaggggc tgtcggagcg gacggtgatc ttcaagcacg
     1321 gcctgcgggc cagcctcacc ccgatcgtga ccccgatcgg cctcgacctg ggggcgctgc
     1381 tgggcggcgc ggtgatcacc gagacggtgt tcaacctcca gggcgtcggc cagtgggcgg
     1441 tcacctcggt gttccagggc gacctgccgg tggtgctggc ggtcacggtg gtggtcgcga
     1501 tcgcggtcac cctgatgaac ctgatcgtcg acatcg
//
LOCUS       DATITW010000484         1561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_263782, whole genome shotgun sequence.
ACCESSION   DATITW010000484 DATITW010000000
VERSION     DATITW010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263782"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VLS86_06750"
     CDS             complement(<1..394)
                     /locus_tag="VLS86_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HSO50229.1"
                     /translation="MPRRILIAKPGLDGHDRGAKVIARALRDAGFEVIYSGLHQTPAQ
                     IAETAVQEDVDGVGLSSLSGAHRTLFPQVVTELSARQASDIVVFGGGVIPDADIPLLA
                     EAGLATVFTPGTPLDEITAWVDANIPSRN"
     gene            455..1552
                     /gene="tal"
                     /locus_tag="VLS86_06755"
     CDS             455..1552
                     /gene="tal"
                     /locus_tag="VLS86_06755"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HSO50230.1"
                     /translation="MTTSRIHGLAAAGVSVWSDQISRSMLDTGELATRIERDAVTGVT
                     SNPTIFAGAIVGSSDYDEQLHDLKTAGASTEEIAKSLMAADIQRGCDLLLGVFRSSGG
                     RDGFVSVEVSPTLAGDSHATVAEARDWVKQISRPNLLVKVPATAEGVPAIRDLIGEGI
                     SVNITLIFSLDRYVEVMEAYLSGLEDFDAAGGDLSTIASVASFFVSRFDTEVDPRLDA
                     IGSEEALALRGVTAIANARVAYGLFEEQFESVRFQALAAEGASVQRPLWASTSTKNPE
                     YPDLIYVDGLVAADTVNTMPLATIDAYQDHGNPSPPAFTADDIEKAKQDLERLGSIGI
                     DYQEVVRVLEDEGVEKFTKSWLELLERVEQA"
BASE COUNT          280 a          484 c          518 g          279 t
ORIGIN      
        1 gattccgcga agggatattg gcatcgaccc atgcggttat ctcatccaac ggtgtccccg
       61 gggtgaacac cgtcgccaga ccggcctcgg cgagaagggg gatgtcggcg tcggggatga
      121 ccccaccacc gaacacgacg atgtcagagg cttggcgcgc ggacaactcg gtgactacct
      181 ggggaaacag ggtgcggtgc gcgccggaaa gtgaggacag cccgacgccg tcgacatcct
      241 cctggacggc ggtctccgcg atctgggccg gggtctgatg gaggccggag tagatgacct
      301 cgaacccggc gtcccgcagc gccctggcga tgaccttggc gccgcggtcg tgaccgtcga
      361 gaccgggctt ggcgatgagg atgcggcgag gcatgcgggg ccacagaggc tagttgccgc
      421 aaggacttcc tcagacctcc cctacccttt ccctatgacc acttccagaa ttcacggtct
      481 ggcggcggcc ggcgtctcgg tctggtcgga tcagatctca cgatcgatgc tcgacaccgg
      541 agagctggcg acccggatcg agcgggacgc ggtgaccggt gtcacctcca accccacgat
      601 tttcgccggg gccatcgtcg gctcctccga ctacgacgag cagcttcatg acctgaagac
      661 ggccggggcc tccaccgagg agatcgccaa gagtctcatg gccgcagaca tccagagagg
      721 ctgcgatctg ctgctcggtg tgttccgctc cagtgggggc cgagacggtt tcgtctcggt
      781 cgaggtatct ccgaccttgg ccggtgacag ccatgccacc gtggcggagg ccagggactg
      841 ggtgaagcag atcagccgtc ccaacctcct ggtcaaagtg ccggcgaccg ccgaaggcgt
      901 ccctgcgatc cgggatctga tcggtgaggg gatctcggtg aacatcaccc tcatcttctc
      961 cctcgatcgg tacgtcgagg tgatggaggc gtatctgtcg ggtctcgagg actttgacgc
     1021 ggcgggcggt gacctgtcca cgattgcgtc tgtggcctcc ttcttcgtgt cccgtttcga
     1081 caccgaggtc gatccccgtc tggatgccat cgggtccgag gaggcgctgg ccctgcgcgg
     1141 agtcaccgca atcgccaacg ccagagtggc ctatggcctg tttgaggagc agttcgaatc
     1201 ggttcgattt caagccctgg ccgctgaggg tgcctcggtg cagcgaccgc tgtgggcgtc
     1261 gacctcaacc aagaacccgg agtaccccga cttgatctac gtcgacgggc tggttgcagc
     1321 cgacaccgtc aacacgatgc cattggcgac catcgacgcc tatcaggatc acggcaatcc
     1381 ctcacccccg gcgttcacag cggacgacat cgaaaaggcc aaacaggatt tggagcgact
     1441 tggctcgatc ggaatcgatt accaggaggt ggtccgggtc ctcgaggatg agggggtgga
     1501 gaagttcacc aagagctggc tcgagcttct ggagcgggtg gagcaggcct gagccccgac
     1561 t
//
LOCUS       DATITW010000485         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_704748, whole genome shotgun sequence.
ACCESSION   DATITW010000485 DATITW010000000
VERSION     DATITW010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704748"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VLS86_06760"
     CDS             <1..340
                     /locus_tag="VLS86_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HSO50231.1"
                     /translation="KLPARKLWIAFGQPSEGRVTVDHGATRALLDGGKSLLAVGIAGV
                     EGSFDAESAVEVAGDDGAVIGKGLVAMSADEMRLAIGRHSSEVGGVVIHRDDFVLLAR
                     SDPSGDRLTG"
     gene            337..1575
                     /locus_tag="VLS86_06765"
     CDS             337..1575
                     /locus_tag="VLS86_06765"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HSO50232.1"
                     /translation="MMDQVGQRARAASHGVRQSTGETRQQALLQMADSLLEAEAALLE
                     ANAADMAQAAAEGISGALVDRLRLTQARVAGMAAGLRSVASLPDPVGRETGGWVLYNG
                     VRLHRVRVPLGVIAVIYEARPNVTADAAGLCIRSGNAVILRGSSYAVRSNLAITSVLR
                     ESLLEVGLPADGVQLLEDTSRDGARALMQATEWVDLLVPRGGPGLIQAVLEDATVPTV
                     IDGAGNCHVYVDASADLEKALAIVVNAKVQRPGVCNAAETLLVHEQVADQFVVDAAKA
                     LISEGVELRGDARARELVPDMAAATESDWATEYLDLILAVRVVPDLESAVEHIRRFGT
                     GHTEAIVTEDRGAADRFVDAIDTAVVAVNVSTRFTDGEEFGFGAEIGNSTQKLHVRGP
                     MGLESLTSERYVLYGDGQVR"
     gene            1676..1933
                     /gene="rpsO"
                     /locus_tag="VLS86_06770"
     CDS             1676..1933
                     /gene="rpsO"
                     /locus_tag="VLS86_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HSO50233.1"
                     /translation="MKTTTEIVAEYGTHESDTGSPEVQVALLSERINHLTEHLREHKH
                     DHHSRRGLLMMVGKRRRLLDYLRREDVDRYRALIAKLGLRR"
     gene            2122..>2493
                     /locus_tag="VLS86_06775"
     CDS             2122..>2493
                     /locus_tag="VLS86_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HSO50234.1"
                     /translation="MPEISVKGTIGGKEFVLSTGKLAGQADGAVVARLGGTEMLVTAT
                     ANKKVREGVDFFPLTVDFEERMYSVGKIPGSFFRREGRPSEQAILTCRLIDRPLRPSF
                     SDNYRNETHVVITSLAVDQENP"
BASE COUNT          433 a          773 c          859 g          428 t
ORIGIN      
        1 caagctcccg gctcggaagc tgtggatcgc gtttggccag ccgtcggagg gaagggtcac
       61 ggtcgaccat ggcgcgaccc gggccctcct cgatggtggc aaatcgcttc tggcggtggg
      121 catcgctggc gtcgagggga gcttcgacgc cgagtcggcg gtcgaggtcg caggagacga
      181 cggggcagtg atcggcaagg gcctggtggc gatgtcggcc gatgagatgc gactggcgat
      241 tgggcggcac agctccgagg tcggaggcgt ggtgatccac cgcgacgatt tcgtcctcct
      301 ggccagaagc gacccttccg gggataggct taccggatga tggatcaggt tgggcagagg
      361 gcgcgggccg cctctcatgg ggtgcgtcag tcgacgggtg agaccaggca acaggcactg
      421 ctccagatgg cggattcgct gctggaggca gaggccgcct tgctcgaggc gaatgccgcc
      481 gacatggcgc aggcggcggc agagggcatc tccggcgccc tcgtcgaccg tcttcgcctg
      541 acccaggccc gggtcgcagg gatggccgcc ggtctgcgca gtgtggcctc gctgcccgac
      601 cctgtgggcc gggagaccgg gggatgggtc ctgtacaacg gggtcaggct gcaccgggtg
      661 cgggtcccgc tcggcgtcat cgcggtgatc tacgaggccc gacccaatgt caccgccgac
      721 gcagccgggc tctgtatcag atcggggaac gcggtgatac tccgcggctc gtcatacgca
      781 gtgcgctcga atttggcgat aacgagcgtc ttgcgcgagt cgctgctcga agtcgggttg
      841 cccgccgacg gggtccagtt gctggaggac accagccgtg acggggcgcg cgccttgatg
      901 caggcgaccg aatgggtcga tctcctcgtc ccccggggcg gcccgggact catccaggcg
      961 gtcctcgagg atgccaccgt gcccacggtg atcgatggcg cgggcaactg ccatgtctac
     1021 gtcgatgctt cggccgatct ggagaaggcg ctggcgatcg tcgtcaatgc caaggtgcag
     1081 cggcccggcg tgtgcaacgc cgcagagact cttctcgtcc atgagcaggt cgctgaccag
     1141 ttcgttgtgg acgcggccaa ggcgctcatc tccgagggcg tggagcttcg cggtgacgcc
     1201 cgcgcccggg agctggtgcc cgacatggcc gcggcaaccg agtcggactg ggccaccgag
     1261 tacctcgacc tcatcctggc ggtccgcgtg gtgcccgatc tcgagtcggc ggtcgagcac
     1321 attcgccggt tcgggaccgg tcacaccgaa gccatcgtca ccgaagaccg cggggccgcg
     1381 gatcggttcg tggatgccat cgacaccgcc gtggtcgcgg tcaacgtgtc cacccggttc
     1441 accgacggcg aggagttcgg cttcggcgcc gagatcggaa actcgacgca gaagctgcac
     1501 gtgcgggggc caatgggtct ggagtccctt accagcgagc gctacgtgct ctatggtgac
     1561 gggcaggtgc ggtgaaggga gagccaatga gcatcagccg gctggttgcg ggttgcgacg
     1621 acggtactgc tagagttgcc gttgtcccca cgtgtcgatt caggtttcac gcgcaatgaa
     1681 gacaaccacc gagatcgtcg ccgagtacgg cacccacgag agcgataccg gctccccgga
     1741 agtgcaagtc gccttgctca gcgagcgaat caaccatttg accgagcatc tccgtgagca
     1801 caagcacgac catcacagtc ggcgcgggct cctgatgatg gtcggtaagc gacgtcgact
     1861 tctcgactac ctgcgcaggg aggatgtcga tcgctatcga gccctcatcg caaagctggg
     1921 gctgcgtcgc tgaacgagtt tcatgggagc ggaagatccg ctctcatgac aaggggcctt
     1981 cgggtcccac atacagtggg tccgaccacg tggctggacc ccaagacaca cgagtcctgc
     2041 cggttgtcag tggcgatgtc tcggtcatct gagacatgac tactgccaac gggctcggct
     2101 cccaatgaca aggagcactt agtgcccgaa atctctgtaa aggggaccat cggcggcaaa
     2161 gagttcgtcc tgagcaccgg caagcttgcc ggtcaggcgg acggcgccgt ggtcgcccga
     2221 ctcggcggca ccgaaatgtt ggtgaccgca actgcaaaca agaaggtgag agaaggcgtc
     2281 gatttcttcc ctctcaccgt cgacttcgaa gagcgcatgt actcggtggg caagatcccc
     2341 gggtcgttct tccgtcgtga gggtcgaccc tccgagcagg cgattctcac ctgccggctc
     2401 atagaccgcc cgctgcgccc ctccttctcc gacaactatc gcaacgagac ccacgtcgtg
     2461 atcacctcgc tggcggtgga ccaggagaat ccc
//
LOCUS       DATITW010000486         1849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_143782, whole genome shotgun sequence.
ACCESSION   DATITW010000486 DATITW010000000
VERSION     DATITW010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143782"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..629
                     /gene="efp"
                     /locus_tag="VLS86_06780"
     CDS             72..629
                     /gene="efp"
                     /locus_tag="VLS86_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HSO50235.1"
                     /translation="MISTNDVRPGMALQLGDGLFTIVEYQHVKPGKGKAFVRMKLKNL
                     ETGQVLERTFRADEDVDQAIIDRRDYQFLYQDDLGFHFMNLDDFGQLALSGDDVGAAA
                     GFLVEGMTASLSLYRGTPIGVELPASVELEVTYAEPAVKGDTRQGATKAVTLQTGIQI
                     QVPLFVEQGDRVKVDTRSGEYLTRA"
     gene            626..1180
                     /gene="nusB"
                     /locus_tag="VLS86_06785"
     CDS             626..1180
                     /gene="nusB"
                     /locus_tag="VLS86_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HSO50236.1"
                     /translation="MSPDPASPMTIPARVTVEELAGALALDVVEVQAVLAARNEPSTP
                     TDVVGADVALEVARALGVSLSIEPRDLALETLYQVEIGGEAPHGAEMKGRVGFLVKGV
                     LEHREDLDHEIESASEHWSVARMPIIDRTILRLGLFELRHSKDTPTAVVVSEAVRLAG
                     TYSTERSGSFVNGVLASLARAVRS"
     gene            1305..1826
                     /gene="pyrR"
                     /locus_tag="VLS86_06790"
     CDS             1305..1826
                     /gene="pyrR"
                     /locus_tag="VLS86_06790"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HSO50237.1"
                     /translation="MNGEDIRRAVRRIAHEILERHRGAEGVVIVGIHTRGVHLAHLLG
                     NLISEFEGVSVPVGELDIGAHRDDRELRAPARPHQTDIPADIARSRVIVVDDVLYTGR
                     TVRAALDALADIGRADQTELAVLVDRGHRELPIRADYVGKNLPTSQDAVVRVRLEPAD
                     PEEGVWVERNGAA"
BASE COUNT          320 a          569 c          660 g          300 t
ORIGIN      
        1 cgggctaggc gccgttggcc tatgagacgg ttaccgtgcc gccggagtcg agcgcaccgg
       61 aaagaccgca catgatcagc accaatgacg tccgaccggg catggccctc caactcggcg
      121 acgggctgtt caccatcgtc gaataccagc atgtgaagcc gggcaagggc aaggccttcg
      181 ttcggatgaa gctgaagaac ctggagaccg gtcaggtcct ggaaaggacg tttcgagcgg
      241 acgaggacgt cgaccaggcc atcatcgatc gtcgcgacta tcagtttctc taccaggacg
      301 atctcggttt tcattttatg aatctggacg acttcgggca gctggcactc agcggagacg
      361 acgtgggtgc agcggccggc tttctggtcg aggggatgac cgccagtctc tcgctgtatc
      421 gggggacccc gatcggggtc gagctccccg cctccgtcga gcttgaggtc acctacgccg
      481 agcccgcggt caagggggac acccgtcagg gggcgaccaa agcggtcact ctccaaaccg
      541 ggatccagat acaggtgccg ctcttcgtgg aacagggtga tcgggtgaag gtcgacacca
      601 ggtccgggga gtacctgacc agggcatgag ccccgaccct gcgtcgccca tgaccatccc
      661 ggcgcgggtg acggtggagg agctcgcagg ggcgctggcc ctggacgtgg tcgaggtgca
      721 ggctgtgctt gcggcccgca acgagccttc gactcccacc gacgtggtag gggccgacgt
      781 ggcgctcgaa gtcgccaggg cgcttggcgt ctcgttgagc atcgagccca gggacctggc
      841 cctggagacg ctgtatcagg tcgagatcgg cggcgaggcg ccgcacgggg ccgagatgaa
      901 gggtcgggtt ggcttcctgg tgaagggggt gctcgagcac agggaggatc tcgatcatga
      961 gatcgagtcg gcctcggagc attggagtgt cgcccggatg ccgatcatcg accggaccat
     1021 tcttcgtttg ggtctgttcg agttgcgtca cagcaaggac acgcccaccg cggtcgtggt
     1081 gtccgaagcg gtccgtctcg ccgggaccta ctccaccgag aggtccgggt cgttcgtcaa
     1141 cggcgtgctt gcctcgctgg ccagggcggt tcgcagctga ggttgggacc gcccggcgac
     1201 ccggctaagg tcgccgcacc tttaaagcac ggtccggtga ggccgggaag gagcgagatg
     1261 ggccacgacc catcggcatc gggaggcgcc cggtgccaca ggtgatgaac ggcgaggaca
     1321 tccggagagc agtccgccgc attgcccacg agatcctgga gcgccaccgc ggcgcagagg
     1381 gcgtggtgat cgtcgggatt cacacccggg gcgtccatct ggcccatctt ctcggcaatc
     1441 tcatctcgga gttcgaagga gtgtcggtgc ccgtcggcga gctcgacatc ggggcacacc
     1501 gggacgatcg cgagctgcgg gcgccggccc gcccccatca gaccgacatc cccgctgaca
     1561 tcgcccggag ccgtgtgatc gtggtggacg acgtcctgta cacgggtcgg accgttcgcg
     1621 ccgcgctcga cgcgctggca gacatcgggc gggcagacca gaccgagctg gcggtgcttg
     1681 tcgaccgtgg gcaccgcgag ctgccgatcc gtgccgacta cgtgggcaag aacctgccga
     1741 ccagccagga cgcggtggtc cgagttcgac tcgagccggc cgatcccgag gaaggggtct
     1801 gggtcgagag gaacggagcg gcatgagcgg gctgttgacg cttcgcgac
//
LOCUS       DATITW010000487         1605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_744630, whole genome shotgun sequence.
ACCESSION   DATITW010000487 DATITW010000000
VERSION     DATITW010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744630"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="VLS86_06795"
     CDS             complement(<1..1537)
                     /locus_tag="VLS86_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HSO50238.1"
                     /translation="MARIPATTSRVVIESESPRIDGGRFAVKRIVGEPITVEADVFCD
                     GHDVVGALLRSRQVGARRWTETPMASIGNDRWMADFTPDLLGQWQFEVEGWADHFATW
                     LDGLDKKVAAGLDVHVDLLIGADLVMAAASRARGKSARTLRKLASTLGDKTVELTDRL
                     EAAFSDELRDTMDAHPDRSRATRSERRYEVTVERERAVFSTWYELFPRSWAVEAGSHG
                     TFVDVTKQLGYIADMGFDVLYLPPIHPIGKTYRKGANNQLSAGPEDPGVPWAIGSTQG
                     GHTAIIPELGTIEDFRMLRDTAQERGLEIALDIAFQCSPDHPWVKEHPEWFRKRPDGS
                     VQYAENPPKKYQDIYPIDFETSDPRGLWIALKGIFDHWIGEGIRIFRVDNPHTKGFPF
                     WEWVIAEIRSEHPDVIFLAEAFTRPKVMYRLAKLGFTQSYTYFTWRNTRSELVEYMTE
                     LGRLDDFFRPSFWPNTPDILTQQLQTGGRPAFIARYVLAATLSSNCGIYGPAFELMDY
                     IPLI"
BASE COUNT          298 a          509 c          506 g          292 t
ORIGIN      
        1 ggatgagcgg gatgtagtcc atcagctcga atgcaggacc gtagatcccg cagttcgagc
       61 tgagcgttgc cgccagcacg tagcgggcga tgaaagccgg tcgaccaccg gtctgcagct
      121 gctgggtgag gatgtcgggt gtgttcggcc agaagctggg ccggaagaag tcatcgagac
      181 gcccgagctc ggtcatgtac tcgacaagct ccgatcgggt gttgcgccag gtgaagtagg
      241 tgtacgactg ggtgaatccc agcttggcca accggtacat caccttgggt ctggtgaagg
      301 cctcggcgag gaagatgacg tcgggatgct cggagcggat ctcggctatc acccactccc
      361 agaatgggaa ccccttggtg tgggggttgt cgacccgaaa gatgcggatc ccctctccga
      421 tccaatgatc gaagatcccc ttcagggcaa tccacaaccc acgcggatcc gaggtctcga
      481 agtcgatcgg gtagatgtcc tgatacttct tcggggggtt ctccgcgtac tgaaccgacc
      541 cgtcgggtcg ctttctgaac cactcggggt gctccttcac ccatggatga tccggcgagc
      601 actgaaaggc gatgtccagg gcgatctcca ggccgcgctc ttgagcggta tcgcgcaaca
      661 tccgaaagtc ctcgatcgtg cccagctcgg ggatgatcgc ggtgtggccc ccctgcgtgc
      721 tcccgatcgc ccacgggacg ccgggatcct cggggccagc cgacaactgg ttgttggccc
      781 ctttgcgata ggtcttgccg atgggatgga tcgggggcag gtagaggacg tcgaatccca
      841 tgtcggcgat atagccgagt tgcttggtga catcgacgaa cgtcccatgc gacccggcct
      901 ccaccgccca cgaccgggga aacagctcgt accaggtgga gaacaccgcc cgctcccgct
      961 ccacggtcac ctcgtatctc cgctcggagc gagtagcccg ggatcggtcg ggatgggcgt
     1021 ccatggtgtc ccggagctca tcggagaagg cagcctccaa ccgatcggtc aactccacgg
     1081 tcttgtcacc gagggtcgat gccagcttgc gcagggtgcg cgccgatttg cccctggccc
     1141 ggctggcggc agccatcacc agatccgccc cgatcaggag atcgacatga acgtccaacc
     1201 cggccgcgac cttcttgtcc agcccatcga gccaggtggc gaaatggtcc gcccaacctt
     1261 cgacctcgaa ctgccactgc cccaacagat ccggggtgaa atcagccatc caccggtcgt
     1321 tgccgataga agccatcgga gtctcggtcc agcgtctggc gcccacctgc cgcgatcgga
     1381 gcagcgctcc gacgacatcg tggccgtcac agaacacgtc ggcctcgaca gtgatcggct
     1441 caccaacgat ccgcttcacg gcgaagcggc cgccatcgat ccgtggcgat tccgattcga
     1501 tgaccacccg gctggtcgtc gctgggatcc ttgccatgta tggcgaatct agcgaggtgt
     1561 tctcgatgcg agggctccgg ggccgaaacg gagtctctcg gagac
//
LOCUS       DATITW010000488         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_343922, whole genome shotgun sequence.
ACCESSION   DATITW010000488 DATITW010000000
VERSION     DATITW010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343922"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VLS86_06800"
     CDS             <1..927
                     /locus_tag="VLS86_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO50239.1"
                     /translation="ATSLVSFERVFEVLDLETDIPDPVHPEALIPVTGRVEFDRVSFR
                     YQSDGPEGLEAVRRFGRRGSAEEESTPSGQAMTREWALHDVDFVAEPGTLTALVGPSG
                     AGKTTISYLVPRLYDVTKGAVLIDGHDVRNVALRDLARAIGVVTQESYLFHDTIAANL
                     RYAKPDATQQELEEAARAANIHDMISKLADGYETIVGERGYRLSGGEKQRIAIARVIL
                     EDPKILILDEATSHLDSRSEALIQEALEKLMRGRTSVVIAHRLSTVRAADQILVIDEG
                     ELVERGNHRALVAAKGLYASLYETQFTEAVSA"
     gene            complement(949..1314)
                     /locus_tag="VLS86_06805"
     CDS             complement(949..1314)
                     /locus_tag="VLS86_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50240.1"
                     /translation="METSQWCDHKPKWVGRVAVSACAECGRVDWLSDSGPIDHAEAMA
                     YLFGSYDLIGHLDALGGPTSSVLAYVAPTAKKRKHLDALPKHVWLAVGSRLWLSHDGQ
                     VLLLATDHQLLIENLTREA"
     gene            complement(1385..1894)
                     /locus_tag="VLS86_06810"
     CDS             complement(1385..1894)
                     /locus_tag="VLS86_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSO50241.1"
                     /translation="MRILIATTGVLPSAPVADLCRRLQTHDQSVTVMTVVRVPRSFLE
                     SLDDDVRRSFLDDSPIGSSDAEAKAARYLEERGQKAVEPIVAALQASGLGAEIRFVEG
                     DDPAEAIINTAEDVGAELVMMGATRRLFTEEAWRSVSARVIEKSHLPVLLVPGTRFED
                     TQEMPILEM"
     gene            1940..>2321
                     /locus_tag="VLS86_06815"
     CDS             1940..>2321
                     /locus_tag="VLS86_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HSO50242.1"
                     /translation="MDSELLNKVTWKIPNALALIGSASGEEWNGMTASWITQLSMEPV
                     LVGVGVDNKAVTHRLISAGGVFSVNLWSAEDTRVFVKFSKPAVREGDTLNGRPVHTGK
                     TGAPIFDDAVAWMDCEVRQAIDFGT"
BASE COUNT          427 a          707 c          793 g          394 t
ORIGIN      
        1 gccacctcgc tcgtctcgtt cgagagggtg ttcgaagtcc tcgatctgga gaccgacatc
       61 cccgaccccg tgcatcccga agcgctcatt ccggtcacag gccgggtcga gttcgaccgg
      121 gtgtcgtttc gctatcagag cgatggcccc gaaggcctgg aggcggtcag gcgctttggc
      181 cggagagggt ctgcggagga ggagtcgacg cccagcggtc aggcgatgac ccgggaatgg
      241 gcgttgcacg acgtcgactt cgtggccgag ccgggcactc tgaccgcgtt ggtcgggccg
      301 tcaggggcgg gaaagaccac catctcctat ctggtgccaa ggctctacga cgtgaccaag
      361 ggggcggtgc taatcgacgg ccacgatgtg aggaacgtcg ccctacgcga cctggcgagg
      421 gcgatcgggg tggtgaccca ggagagctac ctgttccacg acacgatcgc cgccaatctc
      481 cggtatgcca agcccgacgc cacccagcag gagctcgaag aggcggcgcg agcggccaac
      541 atccacgaca tgatctccaa gctggccgac ggctacgaga ccatcgtcgg ggagaggggc
      601 taccggctgt ccggagggga gaagcagcgc atcgccatcg cccgggtgat cctcgaggat
      661 cccaagatcc tgatcctgga cgaggccacc tcacatctcg actcccggtc ggaagcgctg
      721 atccaggagg ccttggagaa gctgatgagg ggtcgtacct cagtggtgat cgcccatcgg
      781 ctctcgacag tgcgcgccgc cgaccagatc ttggtcatcg acgaagggga gctcgtggaa
      841 cgagggaacc atcgggcgct ggtggcggcg aagggtctgt atgcatccct ctacgagact
      901 cagttcaccg aggcggtctc agcttgaggt ggcgtcctcg tctcgagatc aagcttctcg
      961 ggtgaggttc tcgatcagga gctgatgatc ggtggcgagg agcaggacct ggccgtcatg
     1021 ggacagccag agccgggagc cgaccgcgag ccacacatgc ttgggaaggg cgtcgaggtg
     1081 cttgcgcttc ttggccgtgg gggcgacgta ggcgaggacg ctggaagtag gcccgccgag
     1141 ggcgtcgaga tggccgatga ggtcatagct gccaaacaga taggccatgg cctcggcgtg
     1201 atcgatgggg cccgagtcgg agagccaatc gacccgcccg cattcggcac atgcggacac
     1261 cgccacccgt cccacccact tcggcttgtg gtcacaccat tgagaagtct ccatgaccat
     1321 gaagctacga ccggggtgtg acagattttg cttctacgcg acagagggcc ggcgggatac
     1381 cggttcacat ctccaggatc ggcatttcct gggtgtcctc gaaccgggtc ccgggaacca
     1441 ggaggaccgg cagatgggac ttctcgatga cacgagcaga cacgcttcgc catgcctctt
     1501 cggtgaagag gcggcgggtg gcgcccatca tcaccaactc ggcgcccacg tcctcggcgg
     1561 tgttgatgat cgcctctgcc gggtcgtcgc cctccacgaa cctgatctcg gcgcccaacc
     1621 ccgatgcctg gagggcagca actatcggct ccaccgcctt ttggcccctc tcctccagat
     1681 atcgggcggc cttggcctcg gcgtccgagg agccgatcgg tgagtcgtcg aggaaggagc
     1741 ggcgcacgtc gtcgtcgaga gattcgagga aggaacgggg gacgcggacg acggtcatca
     1801 cggtcacgct ctggtcgtgg gtctggagac gtcggcacaa atcggcgact ggtgctgatg
     1861 gtaggacccc ggtcgtggcg atcaggattc gcaccgcctg agattagggt gtcccctctg
     1921 cttttgtcgg ggtgaagacg tggatagcga actgctcaac aaggtcacct ggaagatccc
     1981 caacgccctg gccctgatcg gatcggcgtc gggcgaggag tggaacggga tgaccgcgtc
     2041 ctggatcaca cagctctcca tggagccggt gctggtcggg gtcggagtcg acaacaaggc
     2101 ggtcacccat cgtctcatct cggcaggggg agtcttcagc gtcaatctct ggtcggccga
     2161 ggacacgcgc gtcttcgtca agttctccaa gcccgcggtc cgggagggcg acaccctcaa
     2221 cggcagaccg gtgcacaccg gcaagacagg ggccccgatc ttcgacgatg cggtcgcctg
     2281 gatggattgc gaggtgcgcc aggccataga cttcgggacc c
//
LOCUS       DATITW010000489         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_224178, whole genome shotgun sequence.
ACCESSION   DATITW010000489 DATITW010000000
VERSION     DATITW010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224178"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /gene="alc"
                     /locus_tag="VLS86_06820"
     CDS             complement(<1..917)
                     /gene="alc"
                     /locus_tag="VLS86_06820"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HSO50243.1"
                     /translation="MTDLISTTVGGRIVACNDEFFAEAGNLLKPTVPVWQEDLYTDRG
                     KWMDGWETRRRREPGHDWCVIALGIPGTLSRVTVDTSYFTGNYAEAFSLEACGVGADD
                     GLSRAEWVELIPRSTLDGDTTADFEVDDSHRVTHLRLNIFPDGGVARLRVEGDPVPSI
                     DEVCPESGVTDLASSLVGGVALEASDVHYSDPSYRLRPTDPAGMWDGWETKRRRGPGH
                     DWASFRLGLPGTVESVVVDTRHFKGNAPGWVSVHVSDDGEVWREVVSRAKVGPDKVNT
                     VPVPEPTTAAFLLLDIHPDGGVARLRVMGA"
     gene            complement(914..>2125)
                     /gene="allB"
                     /locus_tag="VLS86_06825"
     CDS             complement(914..>2125)
                     /gene="allB"
                     /locus_tag="VLS86_06825"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HSO50244.1"
                     /translation="GDFSSDLDFGDLVVMAGLVDSHVHVNEPGRTEWEGFATATRAAA
                     AGGSTTIVDMPLNSIPPTVSVAALAEKRAAAAGKLAVDVAFWGGLIPGMEGELAPMVE
                     AGVCGFKSFLVDSGVPEFPPVTAEQLMSALPVLSDLGVPSLIHAEDPAQIHEIVGDPR
                     EYGSYLASRPSSGEAAAIETLNRVVAESGARVHVLHVSSQEAVAALAGAAAAMSGETC
                     PHYLTFLAEDIAPGATPFKCAPPIRTAEHREALWRGLIDGTLSMVVSDHSPAPAAIKD
                     VEHGHFGDAWGGIGSLQLRLPATWTGASERGVQLGDLSRWLARAPAELAGIDDRKGSI
                     TPGLDADFVVWDPDGATVVRGADLEHRHPVTPYEGMRLRGRVEATILGGETVFDGAEV
                     VGGRGRMLRRR"
BASE COUNT          378 a          772 c          655 g          320 t
ORIGIN      
        1 gcacccatca cccgcagccg ggccacaccg ccgtcgggat ggatgtcgag gaggagaaac
       61 gctgccgtcg tcggctccgg caccgggacg gtgttgacct tgtccggccc gaccttcgcc
      121 ctggacacca cctcgcgcca cacctccccg tcatcggaga cgtgcaccga gacccaaccc
      181 ggcgcattgc ccttgaagtg acgggtgtcg acgacgaccg actcgacggt tccgggcagg
      241 ccgagacgaa agctggccca atcgtgaccc gggccacgtc gcctcttggt ctcccagccg
      301 tcccacatcc ccgccgggtc agtcggccgg aggcgatagg acggatcgga atagtggacg
      361 tctgaggcct cgagcgccac accgccgacg agggacgagg cgagatcggt cacgccgctc
      421 tccgggcaca cctcgtcgat cgatggaacc gggtcgccct cgacccggag acgcgccacg
      481 ccgccgtcgg ggaagatgtt gagacggaga tgggtcaccc gatgcgagtc gtcgacctcg
      541 aagtcggcgg tggtgtcgcc atcgagagtg gaccggggga tcaactccac ccactctgcc
      601 ctgctcaaac catcgtccgc gccgacgcca catgcctcca gggagaaagc ctcggcatag
      661 ttgccggtga aataggaggt gtcgaccgtg acccggctca gggtccccgg gatccccagg
      721 gcgatcacac accagtcgtg acccggctca cgccggcgac gggtctccca cccgtccatc
      781 cacttgccac ggtcggtgta caggtcttcc tgccagacgg gaacggtcgg tttgagcaga
      841 ttccccgcct cggcgaagaa ctcgtcgttg caggccacga tccgcccgcc cactgttgtc
      901 gagatgagat cggtcacctt ctcctcagca ttcgtcctct gcccccaacg acttcggccc
      961 cgtcgaagac agtctcgcct ccgaggatgg tcgcttccac cctgccccgc agccgcatcc
     1021 cctcataggg agtgaccggg tgccggtgct cgagatccgc gccccggacc actgtggccc
     1081 cgtccgggtc ccagaccacg aagtctgcgt cgagaccggg agtgatcgac cctttgcgat
     1141 cgtcgatccc ggccagctcg gccggtgccc gggccagcca ccgggacaga tcgccaagct
     1201 gcaccccacg ctcgctggcg ccggtccagg tcgcggggag acggagctga agcgagccga
     1261 tcccacccca agcgtcccca aaatgaccat gttcgacatc cttgatggcg gccggtgccg
     1321 gcgagtgatc ggagaccacc atggacagag tcccgtcgat gagaccccgc cacagggcct
     1381 ctcgatgctc ggcggtcctg atcgggggcg cacacttgaa cggagtcgcc cccggcgcga
     1441 tgtcctcggc gagaaaggtc aggtaatgag gacaggtctc accgctcatg gccgccgcgg
     1501 cgcccgccaa cgccgcgact gcctcctgcg acgagacgtg aagcacatgg actctggcgc
     1561 cgctctcggc gacaacacgg ttcaaggtct cgatcgccgc tgcctccccc gatgacggac
     1621 gcgatgccag ataactcccg tactcccgcg gatcacccac gatctcgtgg atctgggccg
     1681 ggtcctcggc atggatcaac gaaggcactc cgagatcgga cagcacgggg agggccgaca
     1741 tcagctgctc ggccgtcacc ggggggaact cgggcacacc ggagtcgacc aggaacgact
     1801 tgaacccgca gacgcctgcc tccaccatcg gggccaactc accctccatc ccaggtatca
     1861 accctcccca gaaggcgaca tccaccgcca gcttcccggc tgcagccgcc ctcttctccg
     1921 ccagcgccgc gacgctcacc gttggcggga tcgagttgag cggcatgtcg acgatggtcg
     1981 tgctccctcc ggccgcagct gctctggtgg cggtggcgaa tccctcccac tcggtgcgac
     2041 ctggctcatt gacgtggaca tgcgagtcga cgagaccggc catcaccacc aggtcgccga
     2101 agtcgagatc ggaggagaaa tctcc
//
LOCUS       DATITW010000490         1806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_664512, whole genome shotgun sequence.
ACCESSION   DATITW010000490 DATITW010000000
VERSION     DATITW010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664512"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..647)
                     /gene="scpB"
                     /locus_tag="VLS86_06830"
     CDS             complement(87..647)
                     /gene="scpB"
                     /locus_tag="VLS86_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HSO50245.1"
                     /translation="MRELAALEAVLFVTESPVPTGELAEVLELPKKRVEELLDELRHE
                     LEDRGSGLVLRSVAGGWRLYSHPDAYPYLERFSTSATARKLSPAAMETLSVVAYRQPV
                     ARSQISEIRGVDSDSAVRTLERLGLVEEKERLNLPGNPAVYVTTDLFLEKMGLESLLA
                     LPPLADHIPPADTVDSLEETLRPGTV"
     gene            complement(644..1405)
                     /locus_tag="VLS86_06835"
     CDS             complement(644..1405)
                     /locus_tag="VLS86_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HSO50246.1"
                     /translation="MTFHVKTKVFEGPLDLLLQLITKHQVEVTAVGLTEVVGEYVSFL
                     DEMKDLDLEVTSEFLLIAATLIQLKAHSLLPSRGEFDLDDELALIEERDRLLSRLLVC
                     VTFKDVAAVLQRRMQEANRSVPRISGLDQEIAAVQPDLQMPIDSEGLVVLAARVFARE
                     GAEPDLDHLDLDLPSVEAAIDEIRSRVAELTIASFEDLTEHCTRTVEVAAYFLALLEL
                     ARWGLIEVSQEDWLAEIQVRDRGQEHTDLTSEWAS"
     gene            complement(1415..>1806)
                     /locus_tag="VLS86_06840"
     CDS             complement(1415..>1806)
                     /locus_tag="VLS86_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HSO50247.1"
                     /translation="SQPVPDQRHGHYAASTVLAAGEREASVIRTALLHDIGKRHARLG
                     VMGRTFASLSIRLRLPLGRKASSYRDHGEIAAREVVELGCEPLVVDFARHHHGERPVT
                     SPSATWDLLQLADEPPKTRSAARARIT"
BASE COUNT          300 a          595 c          603 g          308 t
ORIGIN      
        1 tccctgatcg cgtcctctgc ggcgcgacgc gacatgagcc ctgcgttggc gatcgccttt
       61 tggaggcgga cgacatccgc catcggtcac accgtcccgg ggcgaagcgt ctcctcgagg
      121 gaatcgacgg tgtcggccgg cgggatgtga tcggcgagcg ggggcagggc caggagcgac
      181 tcgagcccca tcttctccag gaagaggtcg gtggtcacat aaaccgccgg gttgccgggg
      241 aggttgagac gttccttctc ctcgaccagg cccagtcgct ccagggtgcg caccgccgag
      301 tcggagtcga ccccccggat ctccgagatc tggctgcggg cgaccggctg gcggtaggcc
      361 accaccgaga gggtctccat cgcagccggc gacagtttgc gggcggtggc cgacgtggag
      421 aatcgctcta gatacgggta ggcgtcgggg tgggagtaga gccgccatcc cccggccacg
      481 gaccggagca ccagacccga gccgcggtcc tccaattcat gtctcaactc gtcgagaagc
      541 tcttcgaccc gcttcttggg aagctcgagg acctcggcga gctcgccggt gggcaccggc
      601 gactcggtca cgaacagcac cgcttccagg gccgccagct ctctcatgac gcccactccg
      661 aagtcaggtc ggtgtgttcc tgtccccggt ccctcacctg gatctcggcg agccaatcct
      721 cctgggacac ctcgatcaga ccccaacggg ccagctcgag cagcgccagg aagtaggcgg
      781 ccacttcgac ggtgcgggtg cagtgctcgg tcaggtcttc gaagctggcg atggtgagct
      841 cggcgaccct gctcctgatc tcgtcgatgg cagcctccac cgaaggcagg tcgaggtcga
      901 gatgatcgag atcaggctcg gctccctccc gggcgaagac ccgggcggcc agcacgacga
      961 gaccctctga gtcgatcggc atttgcaggt cgggctgcac ggcagcgatc tcctggtcga
     1021 gcccgctgat ccgcggcacg gaccggttgg cttcctgcat tctccgctgc agcaccgcag
     1081 ccacatcctt gaaggtgacg caaaccagga gacgcgacag cagccgatcg cgctcctcga
     1141 tcagggccag ctcgtcatcg agatcgaact cgccccggga gggaagcagg gagtgggcct
     1201 tgagctggat cagggtggcg gcgatgagca ggaactccga ggtgacctcc aggtcgaggt
     1261 ctttcatctc atccaggaac gacacgtact cgccgacgac ctcggtcagc cccactgcgg
     1321 tcacctcgac ctggtgcttg gtgatcagct gcaggagcag gtccagcggg ccctcgaaca
     1381 ctttggtctt gacatggaag gtcatggctg ctgctcaggt tattcgggcc ctggccgcgc
     1441 tcctcgtctt tggcggctcg tcggcgagct ggagcaagtc ccaggttgca gacgggctgg
     1501 tcaccgggcg ttcgccgtgg tgatgtcgtg cgaaatcgac gaccaggggc tcgcatccca
     1561 gttccaccac ttcgcgggcc gcgatctccc cgtggtcccg atagctgctc gcctttcgcc
     1621 ccagcgggag ccggagacga atcgacagcg aggcgaaggt ccttcccatc acaccgagcc
     1681 gggcgtgccg tttcccgatg tcgtggagca gcgcggtcct gatcaccgaa gcctcacgct
     1741 ctccggccgc cagcaccgtg gatgcggcgt agtgaccatg acgctgatcg gggacgggtt
     1801 gggaga
//
LOCUS       DATITW010000491         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_384206, whole genome shotgun sequence.
ACCESSION   DATITW010000491 DATITW010000000
VERSION     DATITW010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384206"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VLS86_06845"
     CDS             complement(<1..915)
                     /locus_tag="VLS86_06845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSO50248.1"
                     /translation="MSTFLTSAERVLQTRAREFIDELIPYEVEAEMNRGELPPEVAKA
                     HHEKSRSLGFLSINMPERMGGPGYGMLEQVLIQEQSGRVTNALGWVVSTGPSWIADVV
                     TDHQMERWIRPTVDGDVHECYAITEEGAGSDVDSIEATARPDGDDYVLNGTKWHVTSA
                     NLAAYVFFQAKLSDGPNAGRHVLFFVDMDAPEVECVRVPEYSHTYGHHHWEMKFTEVR
                     VPKANMVGTEGDGMSFSHAWFRYERLMIAARCCGAAERLIEEATAFAEQRQAYGSSIA
                     DYQAIQFMLADSLTELWAARLMTYETARG"
     gene            1028..>2165
                     /locus_tag="VLS86_06850"
     CDS             1028..>2165
                     /locus_tag="VLS86_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HSO50249.1"
                     /translation="MIRETPFHPRTSAANQTGLWSQWSGYLATEKYQSSEKFEYFAIR
                     NAAAMIDTSPLYKYRIVGHDAEHFLAGVLARDIRKCRPGQAHYTIWCDEAGHVVEDGV
                     ILRLSEDEFLLTAAEPNLAYLRDLIGHESVEITDVSDEFGALALQGPYAREILKTLAP
                     EMEGIGYFHLTPAKVGNASVTISRTGYTGDLGYEVWVGRDDALDVWDAVEEVGAPMGV
                     IPAGQIALLISRIEAGLVLIDVDFYSSRYAWNDDQRTTPIELGMSWMLRDLETDDRAF
                     IGRRAIERELADGTSRWRTVGIVVDWEDWNRVYNERSLVPPKDHTPEHGGMMLYDENV
                     RRIGYTPSFVYSPMAQRHIGIARVRPDHSKLGTKVSLEVTIDHRY"
BASE COUNT          401 a          713 c          670 g          381 t
ORIGIN      
        1 tccgcgggcg gtctcgtagg tcatcagccg cgcggcccac aactcggtga gtgagtcggc
       61 gagcatgaac tggatcgcct gatagtcggc gatgctcgac ccatacgcct gacgttgctc
      121 ggcgaaggcg gtggcttcct cgatgagacg ctccgccgcc ccacaacagc gggcggcgat
      181 catcagacgc tcgtagcgga accaggcgtg ggagaaactc atcccgtcgc cctcggtccc
      241 gaccatattc gccttcggaa ccctgacctc ggtgaacttc atctcccaat gatgatgtcc
      301 ataggtgtgg ctgtactccg ggacccggac gcactccacc tcgggggcgt ccatgtcgac
      361 gaagaacagc acgtgacgac cggcgttggg accgtcgctg agcttggcct ggaagaagac
      421 gtacgcggcg aggttggctg aggtcacatg ccacttggtc ccgttgagca cgtaatcgtc
      481 gccgtcgggg cgggccgtcg cttcgatgct gtctacatcc gagccggcgc cttcctcggt
      541 gatggcgtaa cactcatgca catccccatc gaccgtgggc cggatccaac gctccatttg
      601 atggtccgtg accacgtcgg cgatccacga ggggccggtg gagaccaccc agcccaatgc
      661 gttggtgacc cggccactct gctcctggat caacacctgt tccagcatcc cgtaaccggg
      721 gcctcccatc cgctcgggca tgttgatcga gagaaagccc agcgaccggg acttctcatg
      781 atgggccttc gccacctcag gggggagctc gccgcggttc atctccgcct cgacctcata
      841 ggggatcaac tcgtcgatga actcccgagc gcgggtttgg aggacgcgct cggcagaggt
      901 gagaaaggtc gacacggcct cggacggtag cagcggaacc ctgaacgacg gttcagaaaa
      961 cccctccttt cgcggccgga ggctgtcgat gacgggttaa cctgcctcac cgacccgagg
     1021 tttcccattg atccgtgaga cgccgtttca cccgcggacc agcgccgcca accagaccgg
     1081 tttgtggagt caatggtcgg ggtacctggc aaccgagaag tatcagagct cggagaagtt
     1141 cgagtatttc gccatccgca atgcggcggc gatgatcgac acctcccccc tctacaagta
     1201 ccgcatcgtc ggccacgatg ctgagcactt cctcgccggg gtcctcgcaa gggacatccg
     1261 caagtgccgt cccggtcagg cgcactacac gatctggtgc gatgaagccg gccatgtggt
     1321 cgaagacggg gtcatcctcc gtctctcaga ggacgagttc ctgctcaccg cggccgagcc
     1381 caacctcgca tatctccggg acctgatcgg ccacgaatca gtggagatca cagatgtctc
     1441 cgacgaattc ggcgccctcg ccctgcaggg gccctacgcc cgggagatcc tgaagaccct
     1501 tgccccggag atggagggca tcggctactt ccatctcact ccggccaagg tgggcaacgc
     1561 ttcggtcacc atctcccgca ccgggtacac cggggacctc gggtatgagg tttgggtggg
     1621 ccgcgacgac gccctcgatg tctgggacgc cgtggaggag gttggcgccc ccatgggtgt
     1681 gatccccgcc ggtcagatcg cccttctcat cagccggatc gaagccgggc tggtgctaat
     1741 cgacgtcgac ttctattcgt cccgttacgc ctggaatgac gaccagcgga ccaccccgat
     1801 cgaactcggg atgtcctgga tgctccgcga cctcgagacc gacgaccggg cctttatcgg
     1861 ccgccgggcg atcgagaggg aactggccga cgggacctcc cgctggcgaa cggtgggcat
     1921 cgtggtcgat tgggaggatt ggaatcgggt gtacaacgaa cgaagcctgg tgccgccgaa
     1981 ggaccacacc cccgagcacg gagggatgat gctctacgac gagaatgtca ggcggatcgg
     2041 ctacaccccg agcttcgtct actccccgat ggcgcaacgg cacatcggga tcgcccgggt
     2101 ccgccccgat cactccaagc tgggcaccaa ggtcagcctc gaggtcacga tcgaccaccg
     2161 ttacg
//
LOCUS       DATITW010000492         1885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_264261, whole genome shotgun sequence.
ACCESSION   DATITW010000492 DATITW010000000
VERSION     DATITW010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264261"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VLS86_06855"
     CDS             complement(<1..445)
                     /locus_tag="VLS86_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012015030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSO50250.1"
                     /translation="MSIRVYLVDDHRLFLAGVRAELSEEFSIVGSGFDVDTAIEESRG
                     LNPDVALVDVHMPDGGGIAVVEGVVGTNPDVKFLALSVSDQAEDVIAMIRAGARGYVT
                     KTIEPADLADAVRRINAGDAVFSPRLAGFVLDAFAGVMPSEIDPEL"
     gene            complement(442..1608)
                     /locus_tag="VLS86_06860"
     CDS             complement(442..1608)
                     /locus_tag="VLS86_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HSO50251.1"
                     /translation="MNPKRELVFRRRRSDRVVAGVAGGLADTLGVSDAFVRAAFVTLS
                     MVWGLGVLIYLGMWLMELDRVEDVEVDEVAPHQALGLGVGFAGLMLLLGAFGWWPSNA
                     LVLTVGALAFGVAALTDRNMPGPLASLVDPTVQRPGRIRMLLGVGLLIGGLAVFSANV
                     GPIYEIGPVLLAVALTGVGILVAFGPWVTRLARDLGTERRERIRQEERAEMAAHLHDS
                     VLQTLALIQRSDDPARMSMLARHQESELRDWLYGNVPMDGVDLLSTAFKEAAKRIEAD
                     HQVPIDVVVVGDHTVDESTRALLGAASEAMVNAAKHSGAERVSLYFEADDDELVVYVT
                     DQGKGFDAGTVAPDRRGITQSIKARVHKAGGVAAIDSEPGEGTEVILRMPVPAP"
     gene            1728..>1885
                     /locus_tag="VLS86_06865"
     CDS             1728..>1885
                     /locus_tag="VLS86_06865"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HSO50252.1"
                     /translation="MEQTNTTTEAPEPQTVIDELVRPREGRMIAGVAQGLANRFNLPV
                     LLIRVVFAL"
BASE COUNT          331 a          672 c          591 g          291 t
ORIGIN      
        1 ccagctccgg gtcgatctct gacggcatta ccccggcaaa ggcgtcgagg acgaacccgg
       61 ccagccgtgg ggagaacacg gcgtcacctg cgttgatccg acgcaccgcg tcggccaggt
      121 cggccggctc gatggtcttg gtcacatagc cgcgggcgcc ggcccggatc atcgcgatca
      181 catcctcggc ctggtcggac acggagaggg cgaggaactt cacgtcggga ttggtcccga
      241 ccacgccttc caccaccgcg atccctcccc cgtcgggcat gtggacgtcg accagggcaa
      301 cgtcggggtt gagacccctg ctctcttcga tagcggtgtc gacgtcgaag ccggatccca
      361 cgatggagaa ctcctcggac agctccgccc tgaccccggc gagaaacagg cgatggtcgt
      421 cgaccagata gacacggatg ctcacggtgc aggcaccggc atcctgagga tcacctcggt
      481 gccctctccg ggctcgctgt cgatggctgc cacgcccccg gccttgtgga ccctggcctt
      541 gatcgactgg gtgatcccgc gccggtcggg agcgaccgtg ccggcatcga accccttgcc
      601 ctggtcggtg acgtatacca cgagctcgtc gtcgtcagcc tcgaaataga gcgacactcg
      661 ctccgcaccg gagtgcttgg cggcattgac catggcctcg ctcgccgccc cgagcagggc
      721 cctggtggat tcgtcgacgg tgtggtcccc gaccaccacg acatcgattg gcacctgatg
      781 atcggcctcg atccgcttcg cggcctcctt gaacgcggtg gacagcaagt caaccccatc
      841 catcgggaca ttcccgtaga gccaatcccg caactccgat tcctggtggc gggccagcat
      901 cgacatccgg gccgggtcgt cgctgcgctg aatcagggcc agcgtctgca ggaccgaatc
      961 gtggagatgg gccgccatct cggcccgctc ctcctgacga atccgctccc ttcgctcggt
     1021 gccgaggtcg cgggcgagcc tggtcaccca ggggccgaat gcgaccagga tcccgacccc
     1081 ggtcagagcc accgccagca acacggggcc gatctcatag atcggcccca cattggccga
     1141 gaagaccgcc agcccaccga tgagcagacc aaccccgagc agcatcctga tccgaccggg
     1201 ccgctgcact gtcgggtcga ccagcgacgc cagcgggccg ggcatgttgc gatcggtgag
     1261 ggccgcgacc ccgaaggcga gcgcccctac ggtcaggacc agggcgttcg atggccacca
     1321 gccaaacgcg cccaggagca gcatcagacc ggcaaagccg acccccagtc ccaatgcctg
     1381 gtggggagcg acttcgtcga cctcgacgtc ctcgacccgg tccagctcca tcagccacat
     1441 gcccaggtag atgagcacgc cgaggcccca gaccatgctg agtgtgacga aggcggctct
     1501 gacgaatgcg tcgctcacgc ccagggtgtc ggcgaggcca ccggcgaccc cggcgaccac
     1561 gcggtcactc cttcgtctcc ggaagacgag ctctcgtttc ggattcacgt tgaaatactc
     1621 gcggcccgcg gcgacaacct caatcgggga catcccggag ggcctcgggg acggctcagg
     1681 gtcgtccccg attgatggcc cgcagaccgt gtctttgaat aggtgtcatg gaacagacca
     1741 acacaaccac cgaggccccg gagccccaga cggtgatcga cgagctcgtc cgcccccgcg
     1801 agggtcggat gatcgccggc gtcgcccagg gcttggccaa tcggttcaac ctcccggtcc
     1861 tgctgatcag ggtggtcttc gcgct
//
LOCUS       DATITW010000493         1776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_104709, whole genome shotgun sequence.
ACCESSION   DATITW010000493 DATITW010000000
VERSION     DATITW010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104709"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VLS86_06870"
     CDS             <1..551
                     /locus_tag="VLS86_06870"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HSO50253.1"
                     /translation="PRPFPVITVVPREDWGALSPELARMTTHVISRLTVHHAGSQSGV
                     NGPAQFRGWQGWHMGGQDWPDIAYHLLIGIDGTVYEGRDPAYRGDTGTSYDTTGHFLV
                     VVEGNFEVEQPTAAQIDSLEAVLAWASEHYDVSPATIAGHGDYAATSCPGRNLESRIH
                     SGELRESVETIIEQGGVQLIWP"
     gene            596..>1776
                     /locus_tag="VLS86_06875"
     CDS             596..>1776
                     /locus_tag="VLS86_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HSO50254.1"
                     /translation="MRVRRTHDGLTVRAISGTYVVMLGINLPEADCAGLRGFAIHRID
                     HTENESYWLRGLKTFAETDPGFPPGSSYSTREHPIQGFGWSDYTAKPGHRYTYRVESL
                     VGPPDDLVPSNRVQVTVSTESPADGDHDVYFNRGVAASQAYAQRFGNRRPSEVGQAAY
                     DWLSRGLFEAIRDFIGSATGPGHGLRVAAYEFHYPQVLDALRAALDRGVDVRIVYDAR
                     QAQPGTANTSAVTTAGLAQVSTPRRTHRYISHNKFIVRLANGAPEAVLTGGTNFSEGG
                     IFGHSNVAHVVEDPAVAAEYLRYWGLLQTDPTNRQLKPILTNTVPVPSGYPPRGTLAI
                     FSPRSTTDALEFYSRLAGQADDSLFITFAFGMHPLFQDVYRNAAAPLRYAVMEKKTRA
                     MA"
BASE COUNT          308 a          609 c          547 g          312 t
ORIGIN      
        1 ctcctcgccc gttccccgtg atcacggtgg tgccccgtga ggattggggc gccctgagcc
       61 cggagttggc tcggatgaca actcatgtca tttcccggct caccgtgcat catgcgggca
      121 gccagagtgg cgtcaacgga ccggcacagt tccggggctg gcagggctgg cacatggggg
      181 gccaggactg gccggacatc gcctaccacc tcctgatcgg gatcgatggc acggtttacg
      241 agggccgcga cccggcgtac cggggtgaca ccgggaccag ctatgacacc acaggccact
      301 tcctggtggt cgtcgaaggc aacttcgaag tcgagcagcc caccgcggca cagatcgatt
      361 ccctggaagc ggtcctggcc tgggcttcgg agcactacga cgtctcaccg gcgaccatcg
      421 ccggtcatgg cgattacgcc gctacctcat gtccgggtcg caacctcgaa tcccggatcc
      481 actccggaga gctgcgtgag tcggtcgaga ccatcatcga gcagggtggc gtccagctga
      541 tctggccctg agcgatgcgt agggttgatc cccgaccgtc gagcgaggaa gacgaatgag
      601 ggtcagacga acccacgacg ggctcacggt gcgggccatc agcgggacgt atgtcgtgat
      661 gctcgggatc aacctgcccg aagccgattg tgccgggttg cgaggcttcg ccattcatcg
      721 catcgaccac accgagaacg agtcctactg gctgcggggt ctgaagacct ttgccgagac
      781 cgatcccggg ttccccccgg gctcgagcta ctcgacccgg gagcatccga tccagggatt
      841 cggttggtcc gactacaccg ccaaaccggg ccaccgctac acctaccgcg tcgagtccct
      901 ggtcggccct cctgacgacc tggtgccgtc gaatcgggtg caggtgacgg tttcgaccga
      961 gtctcctgcc gatggtgatc acgacgtcta cttcaatcgc ggggtggccg cctcccaggc
     1021 ctatgcgcag cggttcggca accgacgccc gagcgaggtg ggccaggcgg cgtacgactg
     1081 gctgtcacgc ggtctgttcg aggcgatccg cgatttcatc ggctcggcca ccgggcccgg
     1141 tcacggattg cgagtcgccg cctacgagtt ccactatcca caggtcctgg atgctctccg
     1201 ggctgccctc gaccgtggcg tggacgtccg gatcgtctat gacgcacgcc aggcccaacc
     1261 ggggaccgcc aacacatcag cggtgaccac cgccggtctg gctcaggtgt cgacaccccg
     1321 acgcacccat cggtacatct cgcacaacaa gttcatcgtc cgtctggcca acggcgcccc
     1381 tgaagcggtg ctcaccgggg ggaccaactt ctccgagggc gggatctttg gccactccaa
     1441 cgtcgcccac gttgtcgagg accctgctgt ggccgccgag taccttcgct actggggtct
     1501 gctccagacc gatccgacca accgccaact caagccgatc ctgaccaaca ccgtgcccgt
     1561 gccctcgggt tacccaccaa ggggcaccct cgccattttc agccctcgca gcacgacgga
     1621 tgcgctcgag ttctactcac gtctcgccgg acaggccgac gacagcctct tcatcacctt
     1681 tgcgtttggc atgcatcccc tgtttcagga tgtgtatcgc aacgcagcgg cccctctccg
     1741 ctacgcggtg atggagaaga agaccagggc gatggc
//
LOCUS       DATITW010000494         1913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_745289, whole genome shotgun sequence.
ACCESSION   DATITW010000494 DATITW010000000
VERSION     DATITW010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745289"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VLS86_06880"
     CDS             <1..611
                     /locus_tag="VLS86_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50255.1"
                     /translation="VAADDGGLATLESGDSDETTTTTSAATEADTEEAALEFSQCMRD
                     NGVPDFPDPSFTENGGGIIVGGPDGEGPGFDPQSDEVQAAFEACGELLEGAAFGPGGG
                     NFDATELQDNLLAMAECLRDQGLEVDDPDLSNFGPAAGGPPPDTDEIESEGGQGPGGQ
                     GFSIFGDLDMDDPDVQAAMETCQGEFGFAPPGSNAATEDGEG"
     gene            611..>1913
                     /locus_tag="VLS86_06885"
     CDS             611..>1913
                     /locus_tag="VLS86_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50256.1"
                     /translation="MDDPQSGKAPGSPHELQGNQLTGDPVQNSRPQISRPGAATPTLP
                     TRNDDHHLAKADESALSIQVLRRSRKPLVWSVVGAVGIALLVFVGLRFLGDPVENEGV
                     EAGPVATSEVQVRDLAESEEYTGQLQYDDAIPVAASGSGYLTGLVGDGETIERGDVVY
                     RLSNDPGEAELLTAQQQVASAESQVASASEQSSALSAGPRAAELSSAQASLAQAQLSL
                     QNLTAPASQAEMTAAQAQLGQAEAAYRDLFDGLSSSESNALQGEVTRAGQSYDQAVTG
                     RDLAWITLLTAQASYCGLPSVPVDGLCALGDLPLTDSDISALTVAVQTSLAEDSSTVQ
                     VIQAFITGSNSYDVAVVAVDNAATAWATAQATLAEAAAAPAQADIDQALAAVYQAQDN
                     LNLLLAGPSALEVAQAEANLASAEARLEELLEGATAAERRQA"
BASE COUNT          338 a          633 c          636 g          306 t
ORIGIN      
        1 gggtcgcggc agacgatggt gggttggcca ctcttgagag tggcgattcc gacgagacca
       61 cgacgactac ctctgctgcc acggaagcgg acaccgagga agcggcgctg gaattttcgc
      121 agtgcatgcg cgacaacggc gtgccggatt ttcccgatcc cagcttcact gaaaacggcg
      181 gtggaatcat cgtgggcgga cccgacggcg aaggccccgg attcgacccc cagtccgacg
      241 aagtgcaggc agcattcgaa gcctgcgggg agcttctcga aggggcggcc ttcggtcccg
      301 gtggtggcaa cttcgatgcc actgaactcc aggacaacct tctggcgatg gcagaatgcc
      361 tgcgggacca gggtcttgaa gtggacgacc ccgatctctc caactttgga cctgccgccg
      421 gtggccctcc ccccgacacc gatgagatcg aatctgaggg tggccaaggt ccgggggggc
      481 aggggttctc gatcttcgga gatctcgaca tggacgaccc ggacgtgcag gcggccatgg
      541 agacttgtca gggagagttc ggcttcgcgc cgcccgggtc gaatgctgcc accgaggatg
      601 gtgagggctg atggatgacc cccagagcgg aaaggcaccg ggttccccac acgagttgca
      661 agggaaccag ttgacggggg acccggtcca gaattcaagg ccccagattt cacggccggg
      721 cgcggccact ccgaccctgc cgacccgaaa cgacgatcac cacctcgcca aggccgacga
      781 gagcgcgctt tcgatccagg ttctccgaag gagccgcaag cccctggtct ggtcggtcgt
      841 cggcgcggtg gggatcgccc tgctggtctt tgtcggcctc cgattcctcg gcgacccggt
      901 agagaacgag ggagtggagg cgggacctgt cgcgacgagc gaggtccagg tccgtgatct
      961 ggcagagagc gaggagtaca cgggccaact ccagtatgac gatgcgatcc ccgtggctgc
     1021 ctccggaagc gggtatctaa ccgggttggt cggggacggc gagaccatcg agcggggcga
     1081 tgtggtgtac cggctctcga acgatcctgg agaagccgag ctcctcactg ctcagcagca
     1141 ggtggcatcg gctgagtcac aggtcgccag tgccagcgag caaagctcag ccctctcggc
     1201 cgggcccagg gctgccgaac tctcatcggc gcaagcgtcg ctcgcccagg cccagctgtc
     1261 ccttcagaac ctgacggcac cggcctccca ggctgagatg accgcagccc aggcccagct
     1321 cggtcaggcc gaggccgcct acagggacct gttcgacggt ctcagcagtt cggagtcgaa
     1381 cgctctccag ggcgaagtca ctcgggccgg ccagagctat gaccaggcgg tcaccggccg
     1441 cgaccttgcc tggatcacgc tcctgaccgc tcaggcctcg tattgcgggt tgccttcggt
     1501 cccggtcgat ggcctttgcg ctctcggcga tctgcctcta accgattccg acatctcggc
     1561 tctcaccgtc gcggtgcaga cctcgctggc cgaagattcc tccacggttc aggtcatcca
     1621 ggccttcatc accggcagca actcgtatga cgtcgcggtg gtcgcagtcg acaacgccgc
     1681 taccgcctgg gcaaccgcgc aggcaaccct cgccgaggcg gcagccgcgc ccgcccaggc
     1741 cgacatcgat caggctctgg ccgcggtcta tcaggctcag gacaacctga acctgctcct
     1801 ggccggtcct tccgccctcg aggtggccca ggccgaggcc aacctcgcgt cggccgaagc
     1861 ccgcctcgag gaactgcttg aaggggcgac ggcggccgag cggcggcagg ccg
//
LOCUS       DATITW010000495         1701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_785252, whole genome shotgun sequence.
ACCESSION   DATITW010000495 DATITW010000000
VERSION     DATITW010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785252"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VLS86_06890"
     CDS             <1..590
                     /locus_tag="VLS86_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HSO50257.1"
                     /translation="VEVGGVSQEMEGRHRAGHFTGVATVVAKLFAGLQPDMAMFGRKD
                     AQQLAVLTAMTRDLRCPIQIVHAPLIREPDGFALSSRNVRLGREERERALALSRGLSQ
                     AAEQIDAGERRGGRLEATVRRAIRDLEVDDVMLAAQDTMTPIETLDRPAVLAAAVRVG
                     AVRLIDNIAIDVVDGRVMPDRGLILDEPSRLGTLR"
     gene            611..>1701
                     /locus_tag="VLS86_06895"
     CDS             611..>1701
                     /locus_tag="VLS86_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HSO50258.1"
                     /translation="MTDHAEQDTDPLVAARRERHASILEAGGYPERFERTDLAADLRG
                     RFPDLEPGTQTEVKVSVAGRLMLHRSFGKLQFGTLRDSSGSIQLLVDRGLAGDELAER
                     FETVDLGDWIGAEGVIMTTRKGELSVRVESLVILQKSLRPLPEKWHGLQDLEQRSRHR
                     YLDLMVNEEARQVAMARVLIVSELRRQFVDRGFVEVDTPVLLPQATGAMARPFQTEHH
                     ALGMTMYLRIATELYLKRLVVGGLERVFEMGRIFRNEGIDSTHNPEFTMLEAYQALAD
                     YRDILALIEEVFGAVTERALGETSITYRGEILDLTPPYRRVTMTELISETLGESVDLD
                     TSLEVLRRIGTEAGVEVGDDWTAGRLIAE"
BASE COUNT          297 a          535 c          574 g          295 t
ORIGIN      
        1 cggtcgaggt tggtggcgtc tcccaggaga tggaggggcg ccaccgggcg gggcacttca
       61 ccggtgtcgc caccgtggtc gccaagctct tcgccgggct gcagcccgac atggcgatgt
      121 tcgggcgaaa ggacgcccaa cagcttgcgg tgctcaccgc aatgacccgc gacctccgct
      181 gtccgatcca gatcgtgcac gcccccctga tcagggaacc cgatggcttt gccctctcca
      241 gtcggaatgt ccgtctgggg cgtgaagaac gggagcgcgc cctcgccttg agccgtggtc
      301 tctcccaggc tgcagagcag atcgatgccg gggagcggag gggagggcgt ctcgaggcga
      361 cggtgcgccg agcgatacgt gatctggaag tcgacgatgt catgctcgct gcccaggaca
      421 cgatgactcc gatcgagacc ttggaccgac cagcggtgtt ggcggctgcc gtcagggtcg
      481 gcgccgttcg cctcatcgac aacatcgcaa tcgatgtcgt ggacgggcgc gtcatgcccg
      541 accgcgggct catcctcgat gagccgagcc ggctcggtac cctgcgctga cccccgaagc
      601 tacgagcccg atgacagacc acgcggagca agacaccgac cctctggttg ccgccagacg
      661 ggagcgccat gcgtcgatcc tcgaggcggg cggctatccc gagcgtttcg agcgaaccga
      721 cctcgccgcc gatttgagag ggcgcttccc cgatctggaa ccggggaccc aaaccgaggt
      781 gaaggtgtcc gtggcgggcc ggctgatgtt gcaccgctcg ttcggaaaac tgcagttcgg
      841 cacgctgcgc gacagcagtg ggtcgatcca gctcctcgtt gaccgaggat tggcgggaga
      901 tgagctcgcc gagcgcttcg agacggtcga tctcggcgac tggatcggcg ccgagggggt
      961 gatcatgacc acccgcaagg gcgagctgag cgtcagagtc gaaagcctcg tcatcctcca
     1021 gaagagtctc cggccgcttc ccgagaaatg gcatgggctc caggacctcg aacagaggag
     1081 ccgtcaccgc tacctcgact tgatggtcaa tgaggaggcg cgtcaggtgg cgatggcccg
     1141 cgtcctgatc gtctccgagc tgcgtcgcca gtttgtggac aggggattcg tggaggtcga
     1201 taccccggtg ctgcttcctc aggccaccgg ggctatggca aggcctttcc aaaccgagca
     1261 tcacgccttg gggatgacga tgtacctgcg aatcgccacc gagctctatc tgaagcgact
     1321 ggtggtcggg ggcctggagc gggtcttcga gatggggcgg atctttcgca atgagggcat
     1381 cgactccacg cacaatcccg agttcaccat gctcgaggcg tatcaggccc tcgccgacta
     1441 tcgggacatc ctcgccctga tcgaggaggt cttcggtgcc gtcactgagc gtgccctggg
     1501 tgagacctct atcacctacc gcggtgagat cctcgacctg accccgccct atcgtcgagt
     1561 gacgatgacc gagctgatct cggagacctt gggagagtcg gtcgatctcg acacgtcgct
     1621 cgaagttctg aggaggatcg gcaccgaggc cggggtcgag gtgggcgacg actggaccgc
     1681 cggccgtctc atcgccgaga t
//
LOCUS       DATITW010000496         1895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_425057, whole genome shotgun sequence.
ACCESSION   DATITW010000496 DATITW010000000
VERSION     DATITW010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425057"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="VLS86_06900"
     CDS             <1..1443
                     /locus_tag="VLS86_06900"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HSO50259.1"
                     /translation="PEARARVKERTQRLVELGVRFQENIDEWEDHILVTRDELEGLPP
                     AFAESLETDEETGRLKVTLAYPHMIPFVENAKRRDLREEISFKFNTQAVEANRKILEE
                     AIELRHQIAQEFGVPSWAHHRLEERMAKDPERVSAFYDDLVPPLTRQGETDIAEMAEL
                     LSTDTGDTQVLPWDWRYYDTQQRRTNFGVDPFEVAEYFPLDSVLKGMFHLVQETFGLE
                     FREEPNPDVWHPDARLFAIHDAETGEELAHFYLDLFPREGKYGHAAEFPMILSRRLED
                     GSYQNPVCAMVANFTKPTASAPSLLQHGEVETLFHEFGHVLHQNLGRTELARFSGTSV
                     ERDFVEAPSQIMQHWVWRADVLRRFARHHETGEPIPDRLVEQLVAARQLNVAIHQLRQ
                     LQYGWLDQKIHSAEVDFDLDLVLREGSRLGLMPFHEGTFALASFGHLLGGYDAAYYGY
                     MWSEVFGDDMFSRFELEGVTSPEVGMAYRR"
BASE COUNT          383 a          594 c          587 g          331 t
ORIGIN      
        1 cccgaggcga gggccagggt caaggagagg acgcagcgtc tcgtcgagct cggggtccgt
       61 ttccaggaga acatcgacga gtgggaggac cacatcctcg tcacccgcga cgagctcgaa
      121 ggtctcccgc cggccttcgc cgaatccctc gagaccgacg aggagacggg caggctgaag
      181 gtgacccttg cctaccccca catgatcccg ttcgtcgaga acgccaagcg ccgggacctg
      241 cgcgaggaga tctcgttcaa gttcaacacc caggcggtcg aggccaaccg caagatcctc
      301 gaggaggcaa tcgagctcag acaccagatc gcccaggagt tcggcgtccc cagctgggct
      361 caccaccgtc tcgaggagcg catggccaag gacccggagc gagtatcggc gttctacgac
      421 gacctggtcc cacccctgac caggcagggc gagaccgaca tcgcggaaat ggccgagttg
      481 ctcagcaccg acaccggcga cacccaggtc ctgccatggg actggcgcta ctacgacacc
      541 cagcagcggc gcaccaactt tggcgtcgat ccattcgagg tcgccgagta cttccctctc
      601 gattcggtgc tgaagggcat gttccatctt gttcaggaga ccttcgggct cgaattccgg
      661 gaggagccca atcccgatgt ctggcatccc gacgcccggc tcttcgccat ccatgacgcg
      721 gagaccggcg aagagctcgc ccatttctat ctcgacttgt tcccccgcga gggaaagtac
      781 gggcacgccg ccgagttccc catgattctc agccgccgtc tcgaggacgg ctcatatcag
      841 aacccggtgt gcgccatggt ggccaatttc accaaaccga ccgcgtccgc accatcgctt
      901 ttgcagcacg gcgaggtgga gaccctgttc cacgagttcg gccacgtcct tcatcagaac
      961 ctgggccgaa ccgaactcgc caggttctcc ggcaccagcg tggaacggga cttcgtggag
     1021 gctcccagtc agatcatgca gcactgggtg tggcgcgccg atgtcctccg ccgtttcgcc
     1081 cggcaccacg agaccggcga gccgatcccg gaccgtctcg tcgagcagct ggtggcggcg
     1141 cgtcagctca acgtggccat ccaccaactc cggcaactcc agtacgggtg gctcgaccag
     1201 aagatccaca gcgccgaggt cgacttcgac ctcgatctcg ttctcaggga gggctcccgt
     1261 ttgggcctga tgccgtttca cgagggtacg ttcgcgcttg cctccttcgg gcacctactg
     1321 ggtggttacg acgccgcgta ctacgggtac atgtggtcgg aggtgttcgg tgacgacatg
     1381 ttctcccgct tcgagctgga gggggtcacc agcccggaag ttggaatggc ctaccgccgc
     1441 taggtgttgg aaaagggtgg atcagtggat gccgacgaga tgctgaccag gttcctgggg
     1501 cgggagccgg agaactcggc gtttctgaga aaactcggaa tcgagtgacc ctcaggttct
     1561 ggcgcccgat accgaaacta tcggtagcga gtccgtgatt agcggaagtc ggtgctcgag
     1621 cacctaaact ggtgacaacg ggaaccgatg ttgcgttccc gacgtcccaa tccaggtgag
     1681 cggcgatgat ttatcaccga tttaccggtc ggaccgcaca gtggggatgc gaacaaggat
     1741 cacgaagatc cagtctccga atcaagcggg acgtccaaga gtttcagcga tcaggcccgg
     1801 ttcaaggagg gcccaagaat gacagatatc gttaagggag cggccatcaa aggcatgaca
     1861 cgaccttcga gacggttcaa cgaaggacgg gtgtg
//
LOCUS       DATITW010000497         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_24793, whole genome shotgun sequence.
ACCESSION   DATITW010000497 DATITW010000000
VERSION     DATITW010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24793"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VLS86_06905"
     CDS             <1..1075
                     /locus_tag="VLS86_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSO50260.1"
                     /translation="TGIISDTDAISGLLHAHGALSFWDFAASAPYVAIEMGSKTADFG
                     DYKDAIFISPHKLIGGPGTPGVLVVRRDLLSNEVPVVPGGGTVRYVSPDHHTYISDPE
                     HREEGGTPAIIESIRAGLAFRLKESVGVDTIRRLEHGFIQRAIDAWSQNPNIEILGNP
                     AAERLSIVSFVIRYGARTLHHNFVVALLNDLFGIQARGGCSCAGPYGHRLLGIDLETS
                     SRFEEVVATGCEVIKPGWVRVNFNYFIAEEEFAYILEAVQLIADHGWRLLPDYGYEPE
                     TALWRHRDGLPKTPTSLADVDFAGDETLPLPLTAPIEDLASYLEEAKSILSRDPVPEG
                     ESPDLGPDVERLRWFPNPEEVVA"
     gene            1072..1551
                     /locus_tag="VLS86_06910"
     CDS             1072..1551
                     /locus_tag="VLS86_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50261.1"
                     /translation="MTEPVANPTSEQLDTWHRTFAPLAFNETWSLLDLEAPTREEEEE
                     MLAATLAQRYHWYQIGTPRHRAIADWQVSRVAAVLGYAELARRFGERSLALCLEHDLD
                     AFVTGFAHEALARAAAEVDDIDTFTEHLEAAKENLAEIDDPEERDVLEADLAEMSEP"
     gene            complement(1570..>2780)
                     /locus_tag="VLS86_06915"
     CDS             complement(1570..>2780)
                     /locus_tag="VLS86_06915"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HSO50262.1"
                     /translation="FANVTPRIMRPKRHQGAMPLFTKTFEYRHAKGLVGFISPWNYPF
                     NLSISDALGALASGNAVLIKPDEKTPYSALFGARLLQEAGVPRDLVQIVTGEGASVGR
                     AIVDEVDYIMFTGSTEVGRLLAEMAGRRLIGCSLELGGKNAAIVLPDADLDRTVRELA
                     AGSYANGGQTCVAMERVLVHESIRDDFTRRFVEHSASVPMTAGFDFSSELSSLIDQAQ
                     LERVHAHVEDAVAKGATLLTGGKPRPDVGPLFYEATVLTDVNDDMFMCRGETFGPVTA
                     IYGYTDVAQAIAAANDSEFGLHFSLWGRDTTRALEIARELEAGSVTINDGLVASWGSH
                     EAPMGGMKDSGLGRRHGLEGVLKFTESQTVAVQRLIPAYSPFAGVAPERYTRLIELLT
                     RVFKRLPFYK"
BASE COUNT          485 a          933 c          897 g          465 t
ORIGIN      
        1 gaccgggatc atctccgaca cagatgcgat ctcgggtctg ctccacgctc atggggcgct
       61 gtcgttctgg gattttgccg cctcggcccc ttacgtcgcc atcgagatgg gctcgaagac
      121 cgccgacttc ggggactaca aggacgcgat cttcatctct ccgcacaagc tgatcggtgg
      181 tccgggcact cccggggtgc tggtggtgag aagggacctg ctgtccaacg aggtgcccgt
      241 ggtgccgggt ggaggaacgg tgcgctacgt ctcccccgac caccacacct acatatccga
      301 ccccgagcac cgggaggagg ggggcacacc ggcgatcatc gaatcgatcc gcgccggtct
      361 cgccttccgt ctcaaggagt ccgtgggggt ggacaccatt cgccggctgg agcacggctt
      421 catccagcgt gcgatcgacg cctggtcaca gaacccgaac atcgagatcc tggggaaccc
      481 tgcggcggag cgtctctcca tcgtgagctt cgtcatccgc tacggagcgc gcaccctgca
      541 ccacaacttc gtcgtcgccc tgctcaacga tctgttcggg atccaggcgc gaggggggtg
      601 ctcgtgtgcc ggtccctacg gtcaccgctt gctgggaatc gatctagaga ccagcagcag
      661 attcgaggag gtggtcgcca ccgggtgcga ggtgataaag cccggctggg tgcgggtcaa
      721 cttcaactac ttcatcgccg aagaggagtt cgcctacatc ctcgaggcgg tgcagctgat
      781 cgccgatcac ggttggcggc tgctgccgga ctacgggtac gagccggaga ccgcgctgtg
      841 gcgccatcgg gacggcctgc ccaagacacc gacatcactg gccgatgtcg acttcgccgg
      901 ggacgagaca ttgcccctgc ccctcaccgc tcccatcgaa gatctggcgt cgtacctgga
      961 ggaggcgaag tcgatcctct cccgtgatcc ggttcccgag ggcgagtccc ccgatctcgg
     1021 gccggacgtc gagcgtcttc gatggtttcc gaaccctgag gaggttgtcg catgaccgag
     1081 cccgtcgcca atcccacctc cgagcagctc gatacctggc atcgcacttt cgcccccctg
     1141 gcgttcaacg agacctggag ccttctcgac ctggaggcgc cgacccgcga ggaggaagag
     1201 gagatgctgg ccgcgaccct ggcccagcgg taccactggt accagatcgg gaccccccgc
     1261 caccgggcga tcgccgattg gcaggtgtcg agggtggcgg cggtcctcgg ctacgccgag
     1321 ctggcgcgac gttttggcga gcggtccctg gccctctgcc tcgagcacga cctcgacgca
     1381 ttcgtgaccg gcttcgccca cgaggccctg gcccgagccg ctgcggaggt cgacgacatc
     1441 gacaccttca ccgagcacct ggaggctgcc aaggagaatc tggccgagat cgacgatccc
     1501 gaggaacgag acgtcctcga ggcggatctg gccgagatgt cggagccgtg accgccggag
     1561 cggccgcact cacttgtaga agggcagtct cttgaacacc ctggtgagga gctcgatcag
     1621 gcgggtgtag cgctcgggtg cgacaccggc gaacggcgag taggcaggga tcaacctctg
     1681 caccgccacg gtctgcgact cggtgaactt gagcaccccc tcgaggccgt gccgccggcc
     1741 aagacccgag tccttcatcc cacccatcgg cgcctcgtgt gagccccaac tcgccaccag
     1801 gccatcgttg atggtgaccg agcccgcctc gagctcccgg gcgatctcca gggcccgggt
     1861 ggtgtcccgc ccccagagac tgaagtgaag cccgaactcg gagtcgttgg cggccgcaat
     1921 cgcctgtgcg acatccgtgt acccgtagat cgcggtgacc ggtccaaacg tctccccgcg
     1981 gcacatgaac atgtcgtcgt tgacgtcggt gagcacggtc gcctcgtaga acaacgggcc
     2041 gacgtcggga cgcggcttcc cacccgtgag cagggtcgcc cccttggcga cggcatcctc
     2101 gacgtgggca tggacccgtt ccagttgtgc ctggtcgatc agggagctca gctcgctgga
     2161 gaagtcgaat ccggcagtca taggcaccga agcggagtgc tccacgaatc gcctggtgaa
     2221 atcgtcgcga atcgactcgt ggacgaggac ccgctccatg gcgacgcagg tctggccgcc
     2281 gttggcgtag ctccccgcgg ccaactcccg caccgtgcga tcgaggtccg catcgggaag
     2341 gacaatcgcc gcgttcttcc cccccagctc cagagagcag ccaatcagcc ggcgacccgc
     2401 catctcggcg agaagcctcc cgacctcggt cgacccggtg aacatgatgt agtcgacctc
     2461 atcgacgatg gccctcccga ccgaggcgcc ctccccggtg acgatctgga ccaggtcccg
     2521 gggaacgccg gcctcctgga ggagacgggc cccgaacagc gccgagtacg gggtcttctc
     2581 gtccggcttg atcaggaccg cattgccgga tgccagcgcc ccgagggcat cggagatgct
     2641 caggttgaac gggtagttcc agggagagat gaaaccgacc agccctttgg catgccggta
     2701 ctcgaaggtc ttggtgaaca acggcatggc cccctggtgg cgtttgggac gcatgatccg
     2761 tggtgtcaca ttggcaaaga
//
LOCUS       DATITW010000498         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_545556, whole genome shotgun sequence.
ACCESSION   DATITW010000498 DATITW010000000
VERSION     DATITW010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545556"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VLS86_06920"
     CDS             complement(<1..445)
                     /locus_tag="VLS86_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HSO50263.1"
                     /translation="MPTVAVLPVKSFRQGKGRMAEDLSPDTRARLSQRFAERTAELAA
                     EVGMVPILVAGDDEVASWAVELGFPSIPDPGSGLNAAAESGVRWAGLSSSSWLVLHTD
                     LPLLTRGELEEVVEALAGDRWVIAPSADGGTSALGGFGEVRFAYGP"
     gene            517..801
                     /locus_tag="VLS86_06925"
     CDS             517..801
                     /locus_tag="VLS86_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HSO50264.1"
                     /translation="MPKAVWNGAVLAESDDTVVVEGNHYFPSDSINRDHFRDSDAHTV
                     CYWKGTASYYDVVVDGETNRGAAWHYPNPLPAASKIKGRIAFWRGVQVED"
     gene            complement(809..1249)
                     /locus_tag="VLS86_06930"
     CDS             complement(809..1249)
                     /locus_tag="VLS86_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSO50265.1"
                     /translation="MTWKPRTIAVGVDGSETSTKAAEAAAEVARAWQAKLLIVTVVRP
                     PEGWWGIGGAPPSPEALSTALVEGYEQVLNEVESTIPLEGIDYEMIQELGDPSSKIIE
                     IAEARDVDLILIGRRGAGLAERLMLGSVADQLCHHSPVPVMVVP"
     gene            complement(1309..1767)
                     /locus_tag="VLS86_06935"
     CDS             complement(1309..1767)
                     /locus_tag="VLS86_06935"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HSO50266.1"
                     /translation="MHRVVASYFGSGLILQRVRGADAGSGTVAALVALPFSLWAGSTL
                     GWQAQLGGAALLTVASLWSSRPLAAEVGDAGWIVIDEAAGAFIATIGLLGWPAVIGFV
                     VFRIADISKRPFPGVYQADRLPGALGITLDDTVAGLYGLGLGHAFRVLVF"
     gene            complement(1778..>2081)
                     /locus_tag="VLS86_06940"
     CDS             complement(1778..>2081)
                     /locus_tag="VLS86_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50267.1"
                     /translation="TGEVVDERMLRFSFPPRWHFDVLRGLDYFRSAGSLDDDRLIDGI
                     EVVQRKQLGDGRWPLQQRWPGATWFEMEQVGAPSRWNTLRAMRVLETWRAGRPDQI"
BASE COUNT          382 a          718 c          662 g          319 t
ORIGIN      
        1 ccgggccgta ggcaaagcga acctcgccga atccgccgag cgcggaggtg ccgccgtcgg
       61 ccgaaggcgc gatcacccac cggtcgccgg ccaacgcctc gaccacttcc tcgagctcgc
      121 cacgggtgag gagcggaagg tcggtgtgca gcaccagcca tgagctcgac gagagacccg
      181 cccaccggac ccccgactcc gccgcggcgt tgagtcctga tcccgggtcg gggatggagg
      241 gaaagccgag ctcgaccgcc caactggcga cctcgtcgtc accggcgacc aggatcggga
      301 ccatccccac ctctgcggcc agctcggctg tccgctcggc gaaacgctgg ctgagccgtg
      361 ccctggtgtc gggagagagg tcttcggcca ttcgaccctt gccctgtcga aacgacttga
      421 cgggcagcac tgcgaccgtg ggcacattgc gatcctaggg cgggataatg cgggcagcgg
      481 ccggcagagt cggcccggtc acacagggag tcgaagatgc ccaaagcggt ctggaacggg
      541 gcggtgctcg ccgagagtga cgacaccgtc gtggtggaag gcaaccacta cttccccagc
      601 gactcgatca atcgtgacca ttttcgagac tcggacgcgc acaccgtgtg ctactggaaa
      661 ggcaccgcga gctactacga cgtggtggtc gacggcgaga ccaatcgcgg tgcggcgtgg
      721 cactatccca accccttacc cgcggcctcg aagatcaagg gccggattgc cttctggcgt
      781 ggggtgcagg tcgaggacta ggggatcctc aggggaccac catcaccggc accggcgagt
      841 gatgacagag ttggtcggcg actgatccca gcatcagcct ctccgcaagg cccgcccccc
      901 gtcgcccgat caggatcaga tctacgtcac gagcctcggc gatctcgatg atctttgacg
      961 aagggtcacc aagctcttgg atcatctcgt agtcgatgcc ttcgagcggg atggtggact
     1021 cgacttcgtt gagtacctgc tcgtagccct cgaccaaggc cgtggagagc gcttcgggtg
     1081 atggcggcgc cccaccgatg ccccaccagc cctcaggcgg ccgcaccacg gtcacgatca
     1141 gcagcttcgc ctgccaggcg cgcgcaacct cggcggccgc ctccgcggcc ttggtgctcg
     1201 tttccgagcc gtcgactccg acggcaatgg tccttggttt ccaggtcact gatacctcct
     1261 cgggcagacg atacgccagt tccaggccgt cccggttcgc cggcagtcct agaagaccaa
     1321 gacccgaaac gcatgaccga ggccgagccc gtagagaccg gccacggtgt cgtcgagcgt
     1381 tattcccagc gccccgggca atcggtcggc ctgatacacc ccggggaaag gtcgtttgct
     1441 gatgtccgct atgcgaaaca cgacgaaccc gatgaccgcg ggccatccga gcaagccgat
     1501 ggtggcaatg aacgctccgg ctgcctcgtc gatcacgatc caaccggcgt cgcccacttc
     1561 ggctgccaga ggtcgcgaag accagagtga ggcgacggtg agcagcgctg ccccgccgag
     1621 ctgcgcctgc catccgagcg tggagcccgc ccacagcgag aagggaagcg ccaccagagc
     1681 cgcgactgtg cccgaaccgg cgtctgctcc cctgacccgt tgcaggatca ggccagagcc
     1741 aaagtaggag gcgaccacgc ggtgcacagc ggcgagccta gatctggtcc ggccgcccgg
     1801 cccgccacgt ctcgaggacc cgcatcgcac ggagagtgtt ccaccggctg ggagctccga
     1861 cctgctccat ctcgaaccag gtggcccccg gccagcgttg ctgcagcggc caccgaccgt
     1921 ctccgagttg ctttctctgc accacttcga tgccatcgat gagtcggtcg tcgtcgaggc
     1981 tccccgcgct gcggaagtaa tcgagaccac gcaggacgtc gaaatgccat cggggaggga
     2041 aggagaatct caacatcctc tcgtccacca cctctccggt g
//
LOCUS       DATITW010000499         3365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_625480, whole genome shotgun sequence.
ACCESSION   DATITW010000499 DATITW010000000
VERSION     DATITW010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625480"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VLS86_06945"
     CDS             <1..738
                     /locus_tag="VLS86_06945"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO50268.1"
                     /translation="DVYRRPDASHAPCLIYVHGGSWMRGRPGRQALPMFWNLAEAGWV
                     ILDIRYPLSPVATFPEHLIGVKRAIAWAKKEGRELGVDPDRVSISGGSSGAHLAALAA
                     LTWDHPDLQPGFETEDTSLIACVPFYGIYDLLVRNPTRYDWPFVARDVLKARPQDEPD
                     LYRLGSPIDLVRSDAPPFLVVHGELDSVVLAEESQHFVSATRAAGASVRYHEVIGAQH
                     GFDAIASIRTRAVGRLVSDFLIATARI"
     gene            810..1388
                     /locus_tag="VLS86_06950"
     CDS             810..1388
                     /locus_tag="VLS86_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011457098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HSO50269.1"
                     /translation="MSEGNEQMRKRHVMLAAVIGLVLAACAGDGGDETTTTIVDTPAT
                     TAETTPSTEMMEEMSIIETAEATDGFSTLVAAVEAAGLSETLDGEGPFTVFAPTDDAF
                     AALPEGTVEGLLEDPDALSNILLYHVVPGEVTAEQVTTLESATTAQGADVAISLEGDS
                     VFVDDAQVIMTDIATSNGVIHVIDAVLMPPEA"
     gene            1482..1829
                     /locus_tag="VLS86_06955"
     CDS             1482..1829
                     /locus_tag="VLS86_06955"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HSO50270.1"
                     /translation="MGEPGEDIASIGERLRSDLMLAMRARDDARTRVLRSVLAAIDNA
                     GAVEAPTSSAGTIGYSDVPRRQLDPSTIREVLEAEILEREAGIIEYQGVNRPDEAEIL
                     RAEVATLRSYLVD"
     gene            complement(1831..2769)
                     /locus_tag="VLS86_06960"
     CDS             complement(1831..2769)
                     /locus_tag="VLS86_06960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HSO50271.1"
                     /translation="MTADTSVRDSFRGLVESLRLRIVSSATSPFGHAQYPLARSLDYQ
                     GDPGLLGPGSISWLVLGDAAAFVGGIRGLLIQAAHPEVVAGVGDHSRYREDPLGRLSR
                     TSAYVTATTYGAMPEVEHAVAQVRRVHRIVSGVSSRGVPYDAGDPAFSAWVHNALTES
                     FLVAHRNYGGVALSAAEGDAFVVEQTRIGALLGSDPMPDTAKALSHWIATHPAAAPSP
                     EMREALAFLIDPPLEPGLLIGYKVLFEAAVATVPRRIRDILDVTRTPGAVSVGRAAVA
                     GLRWALGSSPSWQLALIRTGADVPQGRFKQPLPVQT"
     gene            complement(2769..>3365)
                     /locus_tag="VLS86_06965"
     CDS             complement(2769..>3365)
                     /locus_tag="VLS86_06965"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HSO50272.1"
                     /translation="ELALHHSVTATFVPDEPPPSRVWDRITAELDEEPSGAEVMSIEA
                     ARTAKGTPLIWLTSIAALLALVLGVVAVFQQMIIRDFTGPDGIVTAAEEAAEEPGAIV
                     ADFETDDGTVAQVVLTPEGDGFVVPAESLAPLDEERTYQLWVITPDEQVISAGVLGND
                     PQPARFTWTGEVTGFALTRERAGGVVSSEGDVVSVIET"
BASE COUNT          599 a         1114 c         1109 g          543 t
ORIGIN      
        1 gacgtctacc gccgccccga cgccagccac gcaccgtgtc tgatctacgt tcacggaggg
       61 tcgtggatgc gggggcgacc cgggcgtcag gcgctgccca tgttctggaa tctcgccgag
      121 gccggctggg tgatcctgga cattcgctac ccgctgtcgc cggtggccac cttccccgag
      181 cacctgatcg gggtgaaacg cgccatcgcc tgggccaaga aggaaggtcg cgagctcgga
      241 gtcgatccgg atcgggtgag catctccggc ggctcgtcgg gtgcccatct cgccgctctg
      301 gctgcgctca cctgggatca tcccgatctt cagccaggct tcgagacgga agacacctcc
      361 ctcatcgcct gtgtcccctt ctacgggatc tacgacctac tggtccgcaa cccgacgaga
      421 tacgactggc ccttcgttgc ccgggacgtg ctgaaggcga gaccgcagga cgagcccgac
      481 ctgtatcggc tcggctcgcc gatcgacctg gtgcgcagcg acgctccccc gtttctggtg
      541 gtgcacggcg agctcgactc ggtggtgctc gccgaggaat cgcagcattt cgtcagcgca
      601 acgagagcgg caggcgccag cgtgaggtac cacgaggtga taggcgctca acatggcttc
      661 gacgcaatcg cgtcgatccg aactcgagcc gtgggtcggt tggtgtccga ttttctgatc
      721 gccaccgccc gcatctgatc caagccatcc gcttcagccg tcggcgacga accagtgggt
      781 gagtcaaccc ggccgcagat gcgtgccgga tgtctgaagg gaatgaacag atgagaaaac
      841 gacacgtcat gctggccgcg gtcatcggac tggtgctcgc cgcctgtgcg ggcgacggtg
      901 gggacgagac gaccaccacc atcgtcgaca ccccggcaac cactgccgag acgacaccga
      961 gcaccgagat gatggaggaa atgtccatca tcgagactgc ggaggcgaca gatgggttct
     1021 cgactctggt ggccgcggtc gaagccgcgg gcttgagcga gaccctcgac ggtgagggac
     1081 cgttcacggt attcgctccc accgacgatg ctttcgccgc tctgcccgag ggcacggtgg
     1141 aaggcctgtt agaggatccg gatgccctga gcaacatcct cctctaccac gtggtgccgg
     1201 gtgaggtgac cgcggagcag gtcacgaccc tcgagtcggc caccaccgcc cagggagccg
     1261 acgtcgcgat cagcctcgaa ggagactcgg tcttcgtcga cgacgcccag gtcatcatga
     1321 ccgacatcgc gacctcgaac ggcgtgatcc acgtcatcga cgcggtcctc atgcctccgg
     1381 aggcgtgatc cagcccatat cgatctgagg aggcccggtt ggctccgggc ctccttctca
     1441 tttcccccac tgtctcctcg cggtccactt accatcgctg catgggtgag ccaggagagg
     1501 acatcgcatc gatcggtgag cgtcttcgct cggatctgat gctggcgatg agggccaggg
     1561 acgacgcacg gactcgcgtg ctgcgatcgg tcctcgccgc catcgacaat gccggggcgg
     1621 tcgaggcgcc caccagctct gccgggacca tcggttacag cgacgtgcca cgtcgacagc
     1681 tcgacccgag cacgatccgg gaagtgctag aggccgagat cctcgagcgc gaagccggga
     1741 tcatcgaata ccagggtgtc aatcggcccg acgaggcgga gatcctgcga gcagaggtcg
     1801 cgactctgcg cagctacctc gtcgactgag tcacgtctga accggcaggg gttgtttgaa
     1861 tctcccctgg gggacatcgg ccccggtgcg gatcaaggcc aactgccatg agggcgaaga
     1921 gccgagcgcc cacctcagac cggcgaccgc cgcccggccg acgctgacgg cgccgggggt
     1981 cctcgtcacg tccaggatgt cgcgaattcg tcgcggaacg gtggccaccg cagcctcgaa
     2041 gagcaccttg tagccgatca gcagaccggg ctcgagtgga gggtcgatga ggaatgcgag
     2101 agcttcacgc atctcgggcg acggggctgc cgcggggtgg gttgcgatcc agtgcgacag
     2161 tgccttcgcg gtgtcgggca tcgggtccga gccgagcagg gcgccgatcc gggtctgctc
     2221 gaccacgaaa gcgtcccctt ctgcagcaga caacgcgacg ccgccatagt tgcgatgggc
     2281 gaccagaaac gactcggtga gggcattgtg cacccatgcc gagaatgcag ggtcaccggc
     2341 gtcgtatggg accccacgac tcgaaacccc ggagacaatg cggtggacgc gccgcacctg
     2401 ggccaccgcg tgctcgacct ccggcatggc cccataggtg gttgccgtca cgtaggcaga
     2461 ggtgcgggag agacggccca gcggatcctc ccggtatctt gaatggtctc cgaccccggc
     2521 caccacctct ggatgcgcag cctggatcag aagcccgcgg atcccaccca cgaacgccgc
     2581 cgcgtcgcca aggaccagcc aggagatcga gcccgggccg aggagccctg gatcaccctg
     2641 gtagtcgaga cttcgggcaa gtgggtattg ggcatgaccg aagggagacg tggccgatga
     2701 gacgatgcga aggcggaggc tctcgaccaa gccacgaaaa gagtcgcgaa cgctggtgtc
     2761 ggcagtcatc aagtctcgat gacagagacc acgtctcctt cgctgctcac cacgcccccg
     2821 gcgcgctccc tggtgagtgc gaatcccgtc acctccccgg tccaggtgaa ccgcgccggc
     2881 tgggggtcgt tgcccagcac cccggcggat atcacttgct cgtccggggt gatcacccac
     2941 agctgatagg tccgctcctc atcgaggggg gcgaggctct cggccgggac gacgaagccg
     3001 tcgccttcgg gggtgaggac cacttgggcc accgtgccgt cgtcggtctc gaaatcggcc
     3061 acgatcgcac ccggctcctc cgcagcctcc tcggcagcgg tgacaatgcc gtctggcccg
     3121 gtgaagtccc tgatgatcat ctgctggaac acggccacca ccccgaggac gagtgccagc
     3181 agcgccgcga tcgaggtgag ccagatcagc ggggtgccct tggcagtccg agccgcctcg
     3241 atgctcatca cctcggctcc cgagggctcc tcgtccagct ccgctgtgat tcggtcccag
     3301 acccgggatg gtggcggctc atcggggacg aaggtggcag tcaccgagtg atgaagggcc
     3361 agctc
//
LOCUS       DATITW010000500         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_785354, whole genome shotgun sequence.
ACCESSION   DATITW010000500 DATITW010000000
VERSION     DATITW010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785354"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..434)
                     /locus_tag="VLS86_06970"
     CDS             complement(54..434)
                     /locus_tag="VLS86_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HSO50273.1"
                     /translation="MRRPLCLIIAALALVSCQGSPSGSSAEIDLSEFEIQPSLSSFEE
                     GMVALDVVNEGEYGHTLVITDAGGHVLSAGDVIEAGETSTTSIDLVPGDYFFTCRIVA
                     QDDEGNVIDHYEMGMSAEVTVRAG"
     gene            complement(431..>1538)
                     /locus_tag="VLS86_06975"
     CDS             complement(431..>1538)
                     /locus_tag="VLS86_06975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSO50274.1"
                     /translation="AVAAPIDQDLEIDGVAEWTNGDALYLALLPGIANAPSLLLYYDI
                     SELLARRAGALGIQTETLQLAAVAILFAVAALAVMIGRSHSIQRYREMTLEAEFLRKE
                     SESLQIHNVELDVARRNAEAALALAEEKNRIRAEFVLMINHELRTPLTSVVTGAELLL
                     MTDPQGADRYEILEALVHDGRRLQTMIDQLLAVARVENRGLSFAPQPVKFSKVCDAVA
                     LSHSKVIASHSPDCHPGETDPIVMTDLQTLRNLASSLADNAFTHGARNVRIHCSTSEV
                     YEPHFVVGTVPESAVYISISDDGPGIRHEFLPRACEKFEKDSRSSGTGLGLYMARLMA
                     EAMGASLMVWTSPEGTTFTIALPLARVASKLGAA"
BASE COUNT          267 a          472 c          528 g          271 t
ORIGIN      
        1 aggtcacgcg ccgaaggtac cgatcgatgg cgggtctgtg acgccgatcg ttctcagccg
       61 gctctgaccg tcacttcggc ggacattccc atttcgtagt ggtcgatgac gttgccctcg
      121 tcgtcctggg ccacgatccg gcaggtgaag aagtaatccc cggggaccag gtcgatcgac
      181 gtggtggagg tctcgccggc ttcgatgacg tcaccggcgg agagcacgtg ccctccggcg
      241 tcggttatga ccaaggtatg cccgtactcg ccctcgttga ccacatcgag ggccaccatt
      301 ccctcctcga aactggagag cgacggttgg atctcgaact cggagagatc gatctcggcg
      361 gaggacccgg agggtgagcc ctggcaggag accagcgcca gtgcagcgat gatgaggcag
      421 aggggccgtc tcacgccgct ccgagcttcg aggcgacccg ggccaggggc agggcaatgg
      481 tgaaggtggt tccctccgga gaggtccaaa ccatgagcga ggcgcccatc gcctcggcca
      541 tcaggcgggc catgtagagg ccgagaccgg tgcccgagct ccgactgtcc ttctcgaact
      601 tctcacaggc gcgggggagg aactcgtggc ggatccccgg tccgtcatca ctgatcgaga
      661 tgtacaccgc ggactcgggg acggtcccca ccacgaaatg gggctcgtag acctcgctgg
      721 tgctgcagtg gatcctgaca ttgcgggcgc cgtgggtgaa cgcgttgtcg gccagggagg
      781 aggccaggtt tcgcagcgtc tggagatcgg tcatcacgat cgggtcggtc tcgccggggt
      841 gacagtcggg cgagtggctc gctatcacct tggagtggga cagggcgacc gcgtcacaca
      901 ccttggagaa tttcaccggc tggggcgcga aggacagtcc gcggttttca actctggcca
      961 ccgcgagcag ctgatcgatc atggtctgca gcctcctccc gtcgtgaacc agggcttcga
     1021 ggatctcgta gcgatcggct ccctgcggat cggtcatcaa aagcagctcg gccccggtga
     1081 cgacactggt cagcggagtc cgcagctcgt gattgatcat gagcacgaac tcggcgcgga
     1141 ttcggttctt ctcctcggcc aacgcgagcg ccgcctctgc gttgcgacgg gccacgtcga
     1201 gctcgacgtt gtggatctgg agcgactccg actccttgcg caggaactcc gcctccaggg
     1261 tcatctcgcg gtagcgctga atcgagtgag agcgcccgat catgactgcc agcgcggcga
     1321 ccgcgaacag gatggcgaca gccgcgagct gcagggtctc ggtttggatc ccgagcgccc
     1381 cagcgcgtcg tgccagcagc tccgagatgt cgtagtagag aagcagcgat ggcgcgttgg
     1441 cgatgccggg gaggagtgcg agatagaggg catcgccgtt ggtccattcc gccaccccat
     1501 cgatctcgag gtcctggtcg atgggcgcgg cgaccgcc
//
LOCUS       DATITW010000501         1739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_24871, whole genome shotgun sequence.
ACCESSION   DATITW010000501 DATITW010000000
VERSION     DATITW010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24871"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VLS86_06980"
     CDS             <1..317
                     /locus_tag="VLS86_06980"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HSO50275.1"
                     /translation="GGMKQEDIDAAQALADMATIAILHHRASIEAQVLNDQLNHALNS
                     RIVIEQAKGMIAEREGVNMDEACAALRNHARNHHLRLVIVAQDVITGDLDAAALDQLP
                     SR"
     gene            504..1478
                     /locus_tag="VLS86_06985"
     CDS             504..1478
                     /locus_tag="VLS86_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50276.1"
                     /translation="MTQQKHLKALVRARQARTGESYSIARKQILDRLRGVEFSLVIEF
                     KAHERHANQVRFTNDGSELLSGGFSGEAKIWSTEDWEQTGALLGHTQSVNGFAVGETR
                     VITVSSDKTVRLWDLPGRAQIQTLIWAGKQIVAVDLDASGTGVWTGGFDGHVQRWTLE
                     GDKTLDTTVGDRVAAIAAHPSRGWLAVGMVGPDLLVIDGEGGEVIRNASPGEASVSVR
                     WANDGELLVAASPNGSVVAWSTDDWEVARKIDVPGHGMLPIAVDSVTGVLAIGWENHV
                     GLWSPAAEEPLTVVGGLPKGVYSLDFSPDGSRLAQSGADGRIRVWSVG"
     gene            complement(1526..>1739)
                     /locus_tag="VLS86_06990"
     CDS             complement(1526..>1739)
                     /locus_tag="VLS86_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HSO50277.1"
                     /translation="AFVNAFKKDLGVACRVLDYHEHSIGAGTDAQAVCYVEMEVGERT
                     LGGVGIHSDTTTASLQAIVSGINRAS"
BASE COUNT          315 a          537 c          583 g          304 t
ORIGIN      
        1 ctggcggcat gaagcaagaa gacatcgatg ccgcccaggc tcttgctgat atggccacta
       61 tcgccatcct tcatcatcgc gcttccatcg aggcccaagt cctcaacgac caactcaacc
      121 acgctctcaa cagtcgcatc gtcatcgagc aggccaaggg gatgatcgcc gagcgggagg
      181 gggtcaatat ggacgaggca tgtgccgcgt tgcgcaatca cgctcgcaac caccacctcc
      241 ggttggtgat cgtcgcccag gatgtgatca ccggcgacct tgatgctgcg gccctggacc
      301 agcttcccag ccgctagcca gggcgcaacc gcctagagtg tcccaacggc gacgacccgt
      361 cgcccaagcc cctcccgggc actcgcgctg ctcggcactg ggggacaaag gggtccgcga
      421 gcctccgggt cttcggtaac cagtcgatgt ctttcgccgg tcgacgagtg gagcgcgggg
      481 ccccgtcgac ggccggagga gacatgacgc agcaaaagca tctaaaagct ctggtccgag
      541 cgcgacaagc gcggaccggg gagtcctact ccatcgcacg caagcagatc ctggatcgtt
      601 tgcgtggcgt cgaattcagc ctggtcatcg agttcaaggc ccacgagaga cacgccaacc
      661 aggtccggtt cacaaacgac gggtcggagt tgttgtcggg tgggttcagc ggcgaggcaa
      721 agatctggtc caccgaggac tgggaacaga ccggggcgct gcttggccac acccagtcgg
      781 tgaacgggtt cgccgtcggc gagaccagag tcatcaccgt ttcgtcagac aagacggttc
      841 gtctgtggga tcttcccggg cgggcccaga tccagaccct gatctgggca gggaagcaga
      901 tcgtggccgt cgacctcgat gcgtctggca ccggcgtctg gaccggtggg ttcgacggtc
      961 acgtccagcg gtggactctc gagggtgaca agaccctaga caccacagtc ggggaccggg
     1021 ttgcagccat cgcggcacac ccgagccgtg gctggttggc ggttggaatg gtcggcccgg
     1081 atctgttggt gatcgatggc gagggagggg aggtgattcg gaacgcgtcc cccggcgagg
     1141 cttcggtctc ggtgcgctgg gccaatgacg gcgagctcct ggtggcggcg agcccgaatg
     1201 gatcggtggt tgcctggtcg actgacgatt gggaagtcgc ccgcaagatc gatgtccccg
     1261 gacatggaat gctcccgatc gcggtcgact ccgtcactgg cgtgctcgcc atcggatggg
     1321 agaaccacgt gggcctgtgg agtcccgctg cggaggagcc gttgaccgtt gtcggcggct
     1381 tgcccaaggg ggtctactcc ctcgatttct cacccgacgg ttcgcgtctt gcccagagcg
     1441 gtgccgacgg caggatccga gtctggagcg ttggctagta gagcgagagg aggtcgggtg
     1501 acccggcctc cctctcctac cggattcaac tggcgcgatt gatgccggag acgatggcct
     1561 gcagggaggc ggtcgtggtg tcggagtgga tgccaacccc ccccagagtg cgctcgccga
     1621 cttccatctc gacgtagcac accgcctggg catcagtccc ggcgccgatc gagtgctcgt
     1681 gatagtcgag gaccctgcaa gccaccccga gatccttctt gaaggcattg acgaaggct
//
LOCUS       DATITW010000502         1752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_105092, whole genome shotgun sequence.
ACCESSION   DATITW010000502 DATITW010000000
VERSION     DATITW010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105092"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VLS86_06995"
     CDS             <1..1146
                     /locus_tag="VLS86_06995"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HSO50278.1"
                     /translation="TIDGSTGTVYIGRVDVVAPESPPELHQLLEWADEYRVLGVWANA
                     DTAQDAMIARKHGAEGIGLARTEHMFLGERLQVVQRIILSDSEEESAIALKELERVQI
                     SDFESLLEAMDGLPVVIRLLDPPLHEFLPSRFELEHEMRLNAKAGRPIDDLQSMSEHV
                     ARWEEDNPMLGLRGVRLGLMVDHLYRMQARAACHALVNRLQAGGNPKLEIMIPLVADV
                     EELIRMREMIEEEVAAAALDSGLELEVPVGTMIELPRAALTAGFIAKAADFFSFGTND
                     LTQMTYGLSRDDAEGLFLVDYLERGILASNPFQTLDRAGVGRLVRVAAKEGREANPTL
                     TLGLCGEHGGDPESIAFVHGLGLDYVSCSPPRIEGARLAAAQAALAG"
     gene            complement(1143..>1752)
                     /locus_tag="VLS86_07000"
     CDS             complement(1143..>1752)
                     /locus_tag="VLS86_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSO50279.1"
                     /translation="VGVGPPATRIGITGPPGAGKSTLISAIINELRSRSRSVAVLAVD
                     PSSPITGGALLGDRIRMQDHIEDDGVYIRSMASRGHLGGLAVAAEPTIDMLSKAGFDY
                     VLVETVGVGQSEVEVMGLVQVVVLVVGPGWGDQIQAEKAGVVEIADLFVVNKGDRPGV
                     DEVRRVLLGAAGESGPEVLITTAVTGAGGPALVDALDGLAAV"
BASE COUNT          309 a          591 c          566 g          286 t
ORIGIN      
        1 acgatcgatg gctccaccgg gaccgtctac atcggcaggg tcgacgtggt cgcccccgag
       61 tcgccgcccg agctgcatca gctcttggaa tgggcggatg aataccgggt gctgggggtg
      121 tgggcgaacg cagacaccgc ccaggacgcg atgatcgccc gcaaacacgg tgcagaggga
      181 attggcctgg cccgcaccga gcacatgttc ctcggcgaga gacttcaagt ggtgcaacga
      241 atcatcctct ccgactccga agaggagagc gctatcgcgc tgaaggagct ggagcgggtg
      301 cagatcagcg atttcgagag cctgctcgag gccatggacg gcctgcccgt cgtcatccgg
      361 ctcctcgacc cgccgctcca cgagttcctc ccgtcacgct tcgagttgga gcacgagatg
      421 cgtctcaatg ccaaggcggg acgccccatc gacgacctgc agtcgatgtc cgagcatgtg
      481 gcgcgatggg aagaggacaa cccgatgctc gggctgcgcg gcgtccggct cggcctcatg
      541 gtcgaccatc tctaccggat gcaggcccgc gccgcctgtc atgccctggt caatcgactc
      601 caggccgggg gcaacccgaa actcgagatc atgatccccc tcgtcgccga cgtggaagag
      661 ctgattcgga tgcgggagat gatcgaagag gaggtcgccg cggcagcgct ggattcagga
      721 ctcgaactgg aggtcccggt gggaacgatg atcgaactgc cccgtgccgc cttgaccgct
      781 ggtttcatcg ccaaggcagc ggactttttc tcattcggca ccaatgacct cacccagatg
      841 acctatgggc tgagccgtga cgacgccgaa gggctcttcc tggttgacta cctggaacgg
      901 ggcatcctcg cctccaaccc gttccagact ctggaccgtg ccggggtggg ccggctggtg
      961 cgggtggcag ccaaagaggg gcgggaggcc aatccgacgc tgactctggg cctgtgcggg
     1021 gaacatggcg gcgacccgga gtcgattgcc ttcgtccacg ggctgggctt ggactacgtg
     1081 tcatgcagcc cgccgcgaat cgagggggcc cggctggcgg cagcgcaggc cgccctcgcc
     1141 ggttagaccg cagcgagccc gtccagagcg tcgaccagag cgggaccccc tgcgccggtg
     1201 acagccgtgg tgatcaggac ctcaggcccc gactcccctg ccgcccccag caacacccgt
     1261 ctcacttcgt cgacacccgg gcgatcccct ttgttgacga cgaacaggtc tgcgatctcg
     1321 accaccccgg ccttctcggc ctggatctgg tcgccccagc cgggaccgac caccagcacc
     1381 accacttgaa ccagacccat cacctcgacc tccgactggc cgacaccaac cgtttccacc
     1441 agcacgtagt cgaatccggc tttggacagc atgtcgatcg tcggctctgc ggccacggcc
     1501 agccctccga gatgccctcg gctggccatg gaacggatgt agaccccgtc gtcctcgatg
     1561 tggtcctgca tgcggattcg atcaccgagc aatgcgccgc cggtgatcgg gctcgacgga
     1621 tccaccgcga ggacggcaac cgaacgggag cgcgagcgga gctcgttgat gattgcgctg
     1681 atcagggtcg atttcccggc acccgggggg ccggtgatcc cgattcgtgt ggcgggcggg
     1741 ccaactccga cc
//
LOCUS       DATITW010000503         1850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_144690, whole genome shotgun sequence.
ACCESSION   DATITW010000503 DATITW010000000
VERSION     DATITW010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144690"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VLS86_07005"
     CDS             <1..192
                     /locus_tag="VLS86_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSO50280.1"
                     /translation="TGAVVTESVFSWPGTGLLAVDAVRARDFQVVQAVVIVFATIYIL
                     TNLMVDILYAYLDPRIRYQ"
     gene            235..1122
                     /locus_tag="VLS86_07010"
     CDS             235..1122
                     /locus_tag="VLS86_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006653362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO50281.1"
                     /translation="MTARTLPWYSPPRVAGAMRQARRYTLVPLALLTLLLVIPAAFAP
                     LVAPYDPLKGSLAKRLRPPVWQQGGSIDHPLGTDKLGRDILSRIIHGARVSLMVSLVA
                     IFVGGSIGTSLGLISGYFGGWVDALLMRLVDISLSLPTILLALVLVAAVGPSFGTVIT
                     VLVVLLWARYARLVRGETLSIKERDFIARARVAGASHSRIMARYILPNVANSLIVLAT
                     LQVGYVILLESALSFLGAGLPRPTPAWGLMIADGRELIVTAWWVSMFPGLAIMLTVLS
                     LNLLGDWLRDHLDPKLRHV"
     gene            1162..>1850
                     /locus_tag="VLS86_07015"
     CDS             1162..>1850
                     /locus_tag="VLS86_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSO50282.1"
                     /translation="MRPRLVSLLVGLLVVLALIPGSAPTSGSAQEAKGQRLVFASAGF
                     DESNRFWVVARPDHLQYDPFLETLLDVDPKTGEYTSRLAEKWSVSPDSKEWTFILRKG
                     VQFHFGYGPLTAQDVVHSHALMMRPEATATLAGFWRQVEEVKAINDHEVVFRFKRPTT
                     VMPYAASRAGDLRIVSKAQWDKEGLEGFDKRPAGTGSYRYVGRQPGLSVSYERVDNHW
                     RGEKPAFKELV"
BASE COUNT          284 a          615 c          599 g          352 t
ORIGIN      
        1 accggggcgg tggtgaccga gagcgtcttt tcgtggccgg gcaccggatt gctcgcggtg
       61 gatgcggtga gggcccggga cttccaggtc gtccaggccg tggtgatcgt cttcgcgacg
      121 atctacatcc tcaccaatct catggtcgac attctctacg cctatctcga tccccgcatc
      181 cggtatcagt agcggggatc agcgagtgag aaagggggct gcatcatggc aacggtgacc
      241 gcacgaaccc ttccctggta ttcgccaccc agggttgcgg gggcgatgcg ccaagccagg
      301 cggtataccc tggtgccgct tgccctgctc acgttacttc tcgtgatccc ggccgcgttt
      361 gcgccactcg tagcacctta cgaccccctg aaggggtcgc tggccaaacg gctgcggcca
      421 ccggtctggc agcagggtgg ctcgatcgac catcccttgg ggaccgacaa gctcgggcgc
      481 gacatcctga gccggatcat ccacggcgcc cgggtatccc tcatggtgtc cctggtggcg
      541 atcttcgtcg gtggctccat cggcaccagc ctggggttga tctcgggcta cttcggcggc
      601 tgggtggacg ccctcctcat gcgcctggtg gacatctcgc tttccttgcc gacgatcttg
      661 ctggcgctcg ttctggtggc cgcggtgggc cccagcttcg gaacggtgat cacggtcctg
      721 gtggtgctcc tgtgggcgcg ctatgcccgg ctggtccgcg gcgagaccct cagcatcaag
      781 gagcgggact tcattgccag agcgcgggtg gccggagcct cccactcccg catcatggcc
      841 cgctacatcc tccccaacgt cgccaactcc ctgatcgtcc tggcgacgct tcaggtcgga
      901 tacgtcatcc tgctcgagtc cgctctcagc ttcctggggg cggggctccc tcgtcccacc
      961 ccggcgtggg gtctcatgat cgcggatggg cgagagttga tcgtcacggc ctggtgggtc
     1021 tcgatgtttc ccggcctggc catcatgctg accgtgctgt ccctgaacct gctgggcgat
     1081 tggctccgtg accacctgga ccccaagctc aggcacgtct aagagccaag cccaggcacg
     1141 tctaagagga ggttcccgac gatgagaccg agactcgttt cccttctggt cgggctgctg
     1201 gttgtgctgg ccctcatccc ggggagcgcg ccgacatccg gatcggcgca agaggcgaag
     1261 ggccagcgac tggtcttcgc ctccgccggg ttcgacgaga gcaaccgctt ctgggtggtc
     1321 gcccgccccg accatctcca gtacgacccg ttcctggaaa cgctgctcga cgtcgacccg
     1381 aagaccggcg agtacacgtc gcgtctggcc gagaagtggt cggtcagccc cgactccaag
     1441 gagtggacgt tcatcctgcg caagggcgtc cagttccact ttggatatgg cccgctcacc
     1501 gcccaggatg tcgtgcactc ccacgccttg atgatgcgtc cggaagccac cgcgaccctg
     1561 gccgggttct ggcgccaggt cgaggaggtg aaggccatca acgaccacga ggtcgtgttc
     1621 cgcttcaagc gtcccaccac cgtcatgccg tatgcggcgt cgcgcgccgg cgatctccgg
     1681 atcgtcagca aggcccagtg ggacaaggaa gggctggagg ggttcgacaa gcggccggcc
     1741 gggaccggct cctaccgcta cgtgggacgg caacccggcc tgtccgtcag ctacgagcga
     1801 gtggacaacc actggcgagg tgaaaagccc gcgttcaagg agctggtgtt
//
LOCUS       DATITW010000504         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_265166, whole genome shotgun sequence.
ACCESSION   DATITW010000504 DATITW010000000
VERSION     DATITW010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265166"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VLS86_07020"
     CDS             complement(<1..940)
                     /locus_tag="VLS86_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HSO50283.1"
                     /translation="MSFISSIRRLADATPSDRNRVIDLLRAASILVVVFGHWLMAAVT
                     VENGELVPGHLLILADWTHPLTWVFQVMPVFFLVGGYANGLSLRSARRRGQGYGGWLR
                     ARLRRLALPVVPLLLFWTAGGWIGLRLGLDWEVLQLASQVALVPTWFLAAYVLIVTIA
                     PISMWIWERWSWWSIVVGLALAGLCDYLSISLDLVPVGFLNYLLVWGTVHQIGYAWLD
                     GTIDELWKRALMAGVGLVGTLLLVGLGPYPVAMVGLDTSEITNSYPPRVTLAFLGMFQ
                     SGLALMLEKPLRRLMERRGAWVWVVGVSARIMTLYLW"
     gene            987..1613
                     /locus_tag="VLS86_07025"
     CDS             987..1613
                     /locus_tag="VLS86_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HSO50284.1"
                     /translation="MNRPVRSLLIVGGAYVAAQMLADIASLRIIDVGGSAVDAGTLIY
                     PFTFTLRDLVHKIAGKSAARTLIFLAAGINLFMAGFFWLVARLPPDPVTGPQLEFGIA
                     LSPVWGIVIASILAEVVAELIDTEAYSRWVARFGDSRQWGRVLVSNAVAIPVDSAVFV
                     GLATLFGVFPSEIAWSILWVNVIFKFLVTLVSIPWIYWVRPQPLSVST"
BASE COUNT          322 a          582 c          521 g          292 t
ORIGIN      
        1 gccagaggta cagggtcatg atccgggcgc tcaccccgac cacccagacc caagcccctc
       61 gccgctccat caaccggcgc agcggtttct ccagcatcag ggccagaccc gattggaaca
      121 tcccgagaaa ggcgagggtc acccgaggcg ggtaggagtt cgtgatctcc gatgtgtcca
      181 gacccaccat cgccaccggg tacgggccga ggccgaccag gagcagcgtc ccgaccagcc
      241 caaccccggc catgagggcg cgcttccaga gctcatcgat ggtgccgtcg agccaggcgt
      301 aaccgatctg atggaccgtc ccccaaacca gcagatagtt gagaaagcca accggcacca
      361 ggtcgagcga gatcgagagg tagtcgcaga ggccggcgag ggcgagcccg accacgatcg
      421 accaccacga ccagcgctcc cagatccaca tgctgatcgg ggcgatggtg acgatcagga
      481 cataggcggc caggaaccag gtcgggacca gggcgacctg tgaggcaagc tgaagcacct
      541 cccagtcgag ccccagccgc aagccgatcc acccgccggc ggtccagaac aggagcaggg
      601 gcaccaccgg caaggccaga cgacggagtc tcgctcgaag ccagcccccg tagccctgtc
      661 cgcgacgacg ggcggatcgc aatgagagcc cgttggcgta ccctcccacc aggaagaaga
      721 ccggcatcac ctggaacacc caggtgaggg gatgggtcca gtcggccaat atcagcagat
      781 gtcccgggac caactccccg ttctccaccg tcacggcagc catcagccaa tgcccgaaca
      841 ccaccaccaa gatcgacgcc gcccgaagca ggtcgatcac ccggttgcgg tcactcgggg
      901 tggcgtcggc gagacgtcgg atcgaggaga tgaagctcac ccgaccattc tcgctctaac
      961 ttcacgccct tgaaggtgtt cgggcaatga atcggccggt ccgctcgctg ctcatcgtcg
     1021 gcggcgccta cgtggccgcc cagatgctgg ctgacatcgc cagcctccgg atcatcgacg
     1081 tgggaggttc cgccgtggac gccggcaccc tcatctaccc gttcacgttc accctccggg
     1141 atctggtgca caagatcgcg ggcaagtcgg cggcgcgcac gttgatcttc ctcgccgccg
     1201 gcatcaacct gttcatggcg ggcttcttct ggttggtggc ccgtctcccg cccgatccgg
     1261 tcaccggccc tcagctcgag ttcggcatcg ccctgtcacc ggtgtgggga atcgtcatcg
     1321 cctccatcct ggccgaagtc gtcgccgagt tgatcgacac cgaggcctat tcgcgctggg
     1381 tcgcccggtt cggggacagc cgtcaatggg gcagggtgct ggtgtcgaat gcggtggcga
     1441 taccggtcga ctctgcggtg ttcgtgggtc tggcgactct gttcggggtg tttccttccg
     1501 agatcgcatg gtcgatcctc tgggtcaatg tgatcttcaa gttcctggtc actctggtgt
     1561 cgatcccctg gatctactgg gtccgccctc aacccctgtc cgtctcgacc taacggccgt
     1621 ttcgtcgtta atgtgagttg tcggcagttc tctgccgact tccatacaca cacggaggaa
     1681 aaacagcgtg aaacgctatc tgcggtactt cgctctc
//
LOCUS       DATITW010000505         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_706169, whole genome shotgun sequence.
ACCESSION   DATITW010000505 DATITW010000000
VERSION     DATITW010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706169"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VLS86_07030"
     CDS             complement(<1..1001)
                     /locus_tag="VLS86_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSO50285.1"
                     /translation="MKLRRNRTLRGWFSRLRPTVQRRLKIAVPYSLMGLVTLVVTYMF
                     FDTPHWPIWLAFTALFVLLEFFAVEVNDRLLQSSSVMVAMTAGVIFAMTPDSDATFAM
                     ALMGGMALFTPLDFKEKRWFQPLANFGQFVLAGAVAGFLLDLLLGDLGKPTTAHLLQV
                     AVASALAALAYATVQTVLIRRAVKTVFGKDNLQPWSQMHVLFLGQFAMGLLGGLIGAA
                     YLIASRDAVLVLIVGVYAIGHMSLYAFSQLRESHIGSIRGFVKTLEAKDMYTRGHTER
                     VAVFAQMIGEELGFTGTQLEKVRWAALIHDLGKLAVPTELIRKRGRLDDEEYAEMQT"
     gene            1471..2091
                     /locus_tag="VLS86_07035"
     CDS             1471..2091
                     /locus_tag="VLS86_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50286.1"
                     /translation="MDVVAFQEKIGRIEGVEAARVVYGNGHISEIHVLARRTKAPKQL
                     VRDVQSLSQALYGEEIDRRVVSVVQLADGDLEGGFRPALVDVSETLDGSRAEVTVSLR
                     WENDVLHGSSTGAAAASTRARQIADATLDAIRQGIHKEAALAINSMDVPLLGNRRVAV
                     AQVVVVTEASERTLIGCAFVEDDEGRAVVRAVLDALNRFLPDLKLP"
BASE COUNT          428 a          673 c          693 g          375 t
ORIGIN      
        1 tgggtctgca tctcggcgta ctcctcgtcg tcgagccgac cccgcttgcg gatcagctcg
       61 gtggggacgg ccagcttccc caggtcgtgg atgagggcgg cccaccgcac cttctccagc
      121 tgggtcccgg tgaatccgag ctcctcaccg atcatctggg cgaagaccgc cacccgctcg
      181 gtgtggcccc tggtgtacat gtccttggcc tccagcgtct tgacgaatcc ccggatcgac
      241 ccgatatgcg actcccgcaa ctgggagaag gcatagagcg acatgtgacc gatggcatag
      301 accccgacga tgagcaccag caccgcatcc cgacttgcga tcagataggc ggccccgatc
      361 agaccgccga gaagacccat ggcgaactgg cccaggaaga ggacgtgcat ctgggaccag
      421 ggttggagat tgtccttgcc gaacacggtc ttcaccgctc ggcgaatcag cacggtctgg
      481 accgtggcgt aggcgagggc ggccaacgcc gaggccaccg ccacctgcaa cagatgggcg
      541 gtcgtgggct tgccgagatc accgagaagc aggtcgagga ggaatccggc caccgccccg
      601 gcgaggacga attgcccgaa attggccagg ggctgaaacc agcgtttctc tttgaagtcg
      661 agtggggtga acaacgccat gccccccatc agagccattg cgaaggtggc gtcggagtcg
      721 ggagtcatgg cgaagatgac tccggcggtc atcgccacca tcaccgaaga ggactgcagc
      781 agacggtcgt ttacctcgac cgcgaagaac tcgaggagaa cgaagagggc agtgaaggcg
      841 agccagatag gccaatgagg ggtgtcaaag aacatgtagg tgaccaccag ggtcaccagg
      901 cccatgaggg aatagggcac agcgatcttg agccgtcgct gcaccgtggg gcggagacgg
      961 ctgaaccagc ccctaagcgt tctgttcctt ctcagtttca tgggagaccc cgatatgcgt
     1021 ggtgagccgg cgccgttcat cagccgattc gggatgaagc cgagcctgcg gctatcacgg
     1081 ctgcaaggct ggcggcagca gcgagaatgc ggaccaaacg ggccttcatg ctgtgacgct
     1141 caccttcggt tttcgtattt ggccgacgtt gtgttgtcag ccagacgttc cgctccgctg
     1201 gggtagatgg cgtccaattg cttacaaggc cgacgtctga cgccggtctc acccggttcc
     1261 cgttttcacg ggaactcttt acatgtcaaa aaggcccctg acttcgcccg ccacggaccc
     1321 gaggcaggta gtggtcaaag acctcggctg ccccacattc ccatgaatag ctccagcact
     1381 ccgcaatcta gacccggcgg gtaaccggaa caaggggagg tggataacga tcccttgagg
     1441 ggttgacacc cagatgtact ctgacatcgt atggacgtag tcgcattcca ggaaaagatc
     1501 ggtcggatcg agggtgtgga agcggcccgg gtggtgtacg gaaacgggca catatctgag
     1561 atccatgtcc tcgcccgacg caccaaggcc cccaagcaac tggtacgcga cgtccagtct
     1621 ttgagtcagg ccctgtacgg cgaagagatc gatcgccggg tggtttcggt ggtgcaactc
     1681 gccgatggcg acctcgaagg cggcttccga ccggcactcg tcgacgtctc cgagacgttg
     1741 gatggctccc gggccgaggt cacggtcagc ctgcgctggg agaacgacgt tctgcatgga
     1801 tcctcgaccg gcgccgcggc agcatcgacc cgcgctcgcc agatcgccga tgcgaccctg
     1861 gacgcgattc gtcaggggat ccacaaggag gctgccctcg ccatcaactc catggacgtg
     1921 ccgctgctcg ggaatcgaag ggttgccgtg gcccaggtgg tggtggtcac cgaggcgagt
     1981 gagcgaaccc tcatcggatg cgccttcgtc gaggacgatg agggccgggc cgtggttcga
     2041 gccgtcctcg atgccctcaa ccgcttcctg cccgacctca aactgccctg acacacgccg
     2101 tcgtcacccc gcatctgctc tccgccacgg cggcccgtcg agtcctggaa gcaggtggca
     2161 atgcggtcg
//
LOCUS       DATITW010000506         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_305313, whole genome shotgun sequence.
ACCESSION   DATITW010000506 DATITW010000000
VERSION     DATITW010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305313"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VLS86_07040"
     CDS             <1..417
                     /locus_tag="VLS86_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO50287.1"
                     /translation="VQGIYIILATFLISRVNWGDPLGAFLILVTFSAVGAGAGMLMGA
                     VFSNDAQASGIGIVLSLGLAALGGCMLPVELFSPTMQKVAHITPHAWALDGFAELVRR
                     DGTVVDILPELGILAVYATVLLLLAAWRLRIALTRA"
     gene            434..946
                     /locus_tag="VLS86_07045"
     CDS             434..946
                     /locus_tag="VLS86_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50288.1"
                     /translation="MTERESNPSAALGTILSWIGFLWFGFAVLEGMGVLDAVGLSGSA
                     FSNIGSTVCPAIILIGVGRALRRRALASEETIRPDVSQVPGATPPIIPETRPRYDPPR
                     PTTPVAPKPAPRPAAPPKAVVVEPVEPMLPDDQAVRSAPGQGSGSTPRPPKTSQELIE
                     EARRRWGTRP"
     gene            complement(955..>2082)
                     /locus_tag="VLS86_07050"
     CDS             complement(955..>2082)
                     /locus_tag="VLS86_07050"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50289.1"
                     /translation="DIELIPVSIAYDQIQDVPDYALEAQGKGKQKESLGWLFRAVKSL
                     RRRYGDIHIRFGEPVSAAAGLADIEPDEEASIGLQKLAFEVMYRIGQVTPITPTALVT
                     VALLAARGNARNAEQLAEECAGLALFVTARRLPVTEDLTLGDPIRVTGVLDRLAEHGN
                     VSSHAAVGRRAFWLDEEQMVRIAYYRNVIVHFFVPRALAEMALTGLIELEEPTDADVE
                     ASLLRLRDLLKYEFFFQEKQRFLDSIFADVELDAPDWKTSLAANGAGEVLAKMGPAVA
                     QWAVLPFLDAYQIVGDELEESRVPFNEKAFLKGCMDRARMYRIEGRLISGESASLVMF
                     KSALSLADNRELLGGSGDLAQRRRLFAAEIREARELAASGL"
BASE COUNT          338 a          673 c          681 g          390 t
ORIGIN      
        1 gtccagggga tctacatcat cctcgccacg ttcctgatct cccgtgtcaa ctggggtgat
       61 cccttgggtg cgttcctgat cctggtgaca ttctcggcgg tgggcgccgg cgccgggatg
      121 ctgatgggcg cggttttcag caacgatgcc caagcgagtg ggatcggtat cgtcctcagc
      181 ctgggtctgg ccgcactcgg cggttgcatg ctgcctgtcg agttgttctc accgacgatg
      241 cagaaggtcg cccatatcac gcctcacgcc tgggcgctcg acgggttcgc cgagctggtt
      301 cgtcgggacg ggaccgtggt cgacattctc cctgagttgg ggatcctcgc ggtgtacgcc
      361 accgtcctgc tcttgctggc ggcatggcgg cttcggatcg ccctgacccg ggcctgaatg
      421 atgggatggg aaattgacgg agcgtgagtc gaatccgagt gccgcgttgg ggacgatcct
      481 gtcgtggatc ggattcctct ggttcgggtt tgccgtcctc gagggcatgg gagtccttga
      541 tgcagtcggg ctctcgggat cggccttctc caacatcggg agcacggtct gcccggccat
      601 catcctgatc ggggtggggc gggccctccg aagacgggcc ctggcatcgg aggagacgat
      661 ccgtcccgat gtctcccagg tcccgggtgc caccccaccg atcatccccg agacccggcc
      721 caggtatgac ccgccacgtc ccacgacccc ggtcgccccg aaaccggcac cgcggccggc
      781 ggcccctccg aaagctgtgg ttgtggaacc ggtcgagccg atgctccctg atgaccaggc
      841 tgtgcgctcg gctccgggtc agggctcggg ttcgacaccc agaccgccca agacttcaca
      901 ggagctgatc gaggaagccc gccggcggtg ggggactcgc ccctgatcgc aggatcagag
      961 accggatgct gccagctccc gagcctcacg gatctctgcg gcgaagagcc ggcgccgttg
     1021 ggcgaggtca cctgacccgc ccaggagctc gcggttgtcg gccagcgaga gggcggactt
     1081 gaacatgacc agactggcgc tctcacctga gatcagcctg ccctcgatcc gatacatccg
     1141 cgctcggtcc atgcaacctt tgaggaaagc cttctcgttg aacggtacgc gggactcttc
     1201 caattcgtcg cccacgatct gataggcgtc gaggaaggga agcaccgccc actgggcaac
     1261 ggcagggccc atctttgcca gcacctctcc ggcgccgttc gccgccaggc ttgtcttcca
     1321 gtcgggagcg tcgagttcga cgtcggcgaa gatcgaatcg aggaagcgct gtttctcctg
     1381 gaagaagaac tcgtacttga gcaggtcgcg gagtctgagg agggaggcct cgacgtctgc
     1441 gtcggttggt tcttcgagct cgatcaatcc ggtgagggcc atctcggcca gagcccgcgg
     1501 cacgaagaaa tggacgatga cgttgcggta gtacgcgatg cggaccatct gttcctcgtc
     1561 cagccagaat gctcggcggc caaccgcggc gtgactcgac acgttcccgt gctcggccag
     1621 gcgatcgagg accccggtca cccggatggg gtcacccagg gtgagatcct cggtgaccgg
     1681 caggcgtcgg gcggtcacga agagggcgag accggcgcac tcctctgcca gctgttcggc
     1741 attacgggcg ttgcctctcg cggcgaggag agcgaccgtt accaatgcgg tgggggtgat
     1801 cggcgtcacc tgcccgatcc ggtacatcac ctcgaacgcg agcttttgca gcccgatcga
     1861 ggcttcttca tcgggctcga tgtcggccag cccggccgcc gccgaaaccg gctccccgaa
     1921 acggatatgg atgtcgccgt aacgcctccg cagcgacttg accgcccgaa acaaccatcc
     1981 cagcgactct ttctgttttc ccttgccctg agcctcgagg gcatagtccg gcacgtcctg
     2041 gatctggtcg taggcgatcg agaccgggat cagctcgatg tc
//
LOCUS       DATITW010000507         1667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_145097, whole genome shotgun sequence.
ACCESSION   DATITW010000507 DATITW010000000
VERSION     DATITW010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145097"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..857)
                     /locus_tag="VLS86_07055"
     CDS             complement(27..857)
                     /locus_tag="VLS86_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HSO50290.1"
                     /translation="MITKFPVDFFFIIEDAAKAWAADHPDVELITGMGESGSDDEGVI
                     ALIESMVAQGVDGIAITPTGPGIQPALDAAVAAGVDIVLIDNDLPDWTGKAAVVATNN
                     YEGGVLAGEWLAENLQPGSTLGVLEGVAGVPALDDRVLGMIEGLGDADITIVQQTPTD
                     CDQVKGVAAAEDLLTAFPDVDAIYGACGPPIIGALEAIENAGIAPEDIVVVGFDASAD
                     EVSQIKDGNQDASIAQFPPKMGELGMDTLYRVVLGESVEANVDTGTAIVTIDNVADFE
                     "
     gene            complement(1098..>1667)
                     /locus_tag="VLS86_07060"
     CDS             complement(1098..>1667)
                     /locus_tag="VLS86_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO50291.1"
                     /translation="MEIDGDPVHIQSPADARRLGIETVYQDLALFDNLSPSANFYAGR
                     ELAGPGWLPFGMRLLRRRSMDEATRDVLNRLHVGLPDLDAAVGLMSGGQRQAVAVARA
                     TAFTSRIVILDEPTAALGVREARGVLDLIRRLRDDGKAVIVISHAMDHVMEIADRAIV
                     MRRGRTVGELVPTPESQQRIVSLIVGGEG"
BASE COUNT          291 a          562 c          500 g          314 t
ORIGIN      
        1 cacccaccgc tcccccgatc gggctcctat tcgaagtccg ccacgttgtc gatcgtcacg
       61 atggcggtcc cggtgtcgac gttcgcttcg acggattcgc ccaggaccac ccggtagagg
      121 gtgtccatac cgagctcacc catcttgggc gggaactgtg cgatggacgc atcctggttc
      181 ccgtccttga tctggctcac ctcgtcggcg gacgcatcga agccgaccac gactatgtct
      241 tcgggggcta tcccggcgtt ctcgattgcc tcgagagctc cgataatcgg cggtccacac
      301 gccccgtaga tggcatcgac gtctggaaac gccgtcaaca ggtcttcagc ggcagccacg
      361 cccttgacct ggtcgcagtc ggtgggagtc tgctgcacga tcgtgatatc ggcgtcgccg
      421 agaccctcga tcatcccgag gacacggtca tccaacgcgg gcacgccggc caccccttcg
      481 agcactccca gggtcgatcc gggttgcagg ttctcggcca gccattcacc cgccaggaca
      541 cctccctcat agttgttggt ggccaccact gcggcctttc cggtccaatc ggggaggtcg
      601 ttgtctatca ggacgatgtc gacaccggct gccaccgccg cgtcaagcgc gggctgaatg
      661 ccggggcctg tcggtgtgat ggcgatgccg tcaaccccct gggcaaccat cgactcaatg
      721 agcgcgatga cgccttcgtc gtccgagcct gactcgccca tgccggtgat aagctcgaca
      781 tcgggatggt ctgcggccca ggcttttgct gcatcctcga tgatgaagaa gaagtcgacc
      841 gggaacttcg tgatcaaccc aagcttgtag ctctcgcctg cggcgcttgt agccggtgct
      901 tcagtaacgg tggtttcacc agcaggttca gtgctggcag aaccgtcacc gccgcaggcg
      961 gctacaagca tcaccaccgc cagagcggct acccatcgaa tccgattcca tttcattgct
     1021 atctcctatc cggggtggcg tcgtgcttgc ccggccgcgc cacccacacg cggtcgggat
     1081 ccgaaggact caattcctca accttcacct cccacgatca gagagacgat tcgctgctgg
     1141 gactccggag tcggcacaag ctctccaact gtccgcccgc gacgcatcac gatggcgcga
     1201 tcggcgatct ccatcacgtg gtccatcgcg tgggagataa cgatcaccgc cttgccgtca
     1261 tcgcgcagac gtcgaatcag gtcgaggact cccctcgcct cgcggacgcc gagcgcggct
     1321 gtcggttcgt caaggatgac gatccgggag gtaaacgcgg tcgcccgggc gactgccact
     1381 gcctggcgtt gacctcccga catgagacca accgcggcgt ccaggtcggg cagcccgacg
     1441 tgcagccgat tgaggacatc ccgggtcgcc tcgtccatcg atcggcggcg cagtagtcgc
     1501 atgccgaatg gcagccagcc ggggccggcc aattcccggc cggcatagaa gttggcagag
     1561 gggctgaggt tgtcgaacag agccaggtcc tggtagacgg tttcgatacc gagccgtctg
     1621 gcatccgccg ggctctggat gtgaaccggg tcgccgtcaa tctccat
//
LOCUS       DATITW010000508         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_65383, whole genome shotgun sequence.
ACCESSION   DATITW010000508 DATITW010000000
VERSION     DATITW010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65383"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1710
                     /locus_tag="VLS86_07065"
     CDS             <1..1710
                     /locus_tag="VLS86_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HSO50292.1"
                     /translation="LPLLIGARDVAFPRLNALSWWIFFFGAIFLYSTFFFGTSMAPVG
                     VDGIGGIDGNLAPGGGPGSFNFLADSPDGGWFGYQPNAGPLYSSGTAMDYWALGLQII
                     GLASLVSAVNFIVTVLNMRAKGMRLLRMPVFAWMTTVTSFLLLFSLPIIAVALFMVTF
                     DRQFGSLFFAATDGGDPILWQHLFWLFGHPEVYILILPAMGIVSEILPTFSRKPLFGY
                     SAVVFAGAAIGFLGFGVWAHHMFASGITPVAQAAFGLATMTIAIPTGIKIFNWLGTMW
                     MGRIRLATPMLFAIGFVAMFTIGGLSGVTHAVVPSDWQQTDTYYIVAHFHYVIFGGAI
                     FGLFGGLYYWFPKLTGRVMSERLGKTHFWLMLLGFNLTFGPMHWLGLQGMVRRTWKYA
                     PEMGLEPWNIVVTIGGFVIALSILVFMYNWITSRRRGDASGLDPWDARTVEWMVPNPS
                     PEYNFAVPPVVRSLDHFWHLKYDEDEEGRAVRKDDADEIVARIEYEQANPESPIHLPN
                     PSYFPLLVALGLPIIFYGVIYHEALWGKALMLVGVIITLSAVIGWGMEPLEEPRPSSA
                     GAH"
     gene            1710..2375
                     /locus_tag="VLS86_07070"
     CDS             1710..2375
                     /locus_tag="VLS86_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HSO50293.1"
                     /translation="MTTAAHDEASADGHGDHTLEDAHHPEHESTGVENRKLAMWAFLS
                     SEFLFFGALITNYLLYSSRDFAQGPTPAQIYDIPFTSVSSFVLLMSSLTMVLAHNAVS
                     RGDQGATRTWLLATAMLGSVFLAGQVFEFTEFITLEGMTLRTTPAASAFYVLTGFHGT
                     HVAIGVIMLLALFGLSKRLGGLSDKQFLNLELVGLYWHFVDIIWIVIFTVVYLLTAVP
                     GGG"
     gene            2377..>2561
                     /locus_tag="VLS86_07075"
     CDS             2377..>2561
                     /locus_tag="VLS86_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50294.1"
                     /translation="MTQEHQIHHPSPVQYVKIAIGLAVLTAIEVALFYINESLQLGWI
                     NTAALLTLAFLKFVIVIG"
BASE COUNT          400 a          846 c          751 g          564 t
ORIGIN      
        1 ctcccgctgt tgatcggcgc ccgtgacgtt gccttccccc ggctcaatgc cctctcctgg
       61 tggatcttct tcttcggggc gatcttcctc tactccacct tcttcttcgg cacctcgatg
      121 gccccggtcg gggtcgacgg cattggaggc atcgacggca acctggcgcc ggggggtggt
      181 cccggcagct tcaacttcct cgccgactcg ccagacggag gatggttcgg ctatcagcca
      241 aacgccgggc cgctgtactc atcggggacg gcaatggact attgggcgct cgggctccag
      301 atcatcgggc ttgcctccct ggtctcggcg gtgaacttca tagtgaccgt gctcaacatg
      361 cgggcgaagg ggatgcggct gttgcggatg ccggtcttcg catggatgac gacggtgacc
      421 tcgttcctgc tcttgttctc gctcccgatc atcgcggtcg ccttgttcat ggtcacgttc
      481 gatcgccagt tcgggtcgtt gttctttgcg gccaccgatg gtggcgaccc gatcctgtgg
      541 cagcacctgt tctggctgtt tggccatccc gaggtctaca tcctgatctt gcccgcaatg
      601 ggcatcgtct ccgagattct cccgaccttc tcccgcaagc cgctgttcgg ctactcggcg
      661 gtcgtgttcg caggagccgc gatcggcttt ctcgggttcg gggtgtgggc ccatcacatg
      721 ttcgcctcgg gcataacccc ggttgcccag gcggcgtttg gtctggcgac catgaccatc
      781 gccatcccca ccgggatcaa gatcttcaac tggctgggca cgatgtggat ggggcggata
      841 cgtctcgcca cgccgatgtt gttcgccatc gggttcgtgg ccatgttcac gatcggcggg
      901 ctatccggcg tgacccacgc agtcgtcccc tccgactggc agcagaccga cacctactac
      961 atcgtggccc actttcacta cgtgatattt ggtggcgcca tctttggcct gttcggaggt
     1021 ctctattact ggtttccgaa gctgacggga agggtgatga gcgagcggct gggcaagacc
     1081 cacttctggc tcatgctgtt ggggttcaat ctgacctttg ggcccatgca ctggctgggg
     1141 ctgcagggca tggttcgccg aacctggaag tacgcccccg agatgggtct ggagccatgg
     1201 aacatcgtgg tgacgatcgg tgggttcgtc atcgccctct ccatccttgt cttcatgtac
     1261 aactggatca ccagccggcg tcggggcgac gcctccgggc tcgacccgtg ggacgctcgc
     1321 accgtggagt ggatggtccc caacccgtca cccgagtaca actttgccgt gcctccggtg
     1381 gtcaggagcc tcgaccattt ctggcacctc aagtacgacg aggacgagga gggccgggcg
     1441 gtccgcaagg atgacgcaga cgagatcgtg gcccggatcg aatacgagca ggcgaacccg
     1501 gagagcccga tccaccttcc caacccctcc tacttccccc tactggtggc cctcggcctc
     1561 cccatcatct tctacggcgt catctaccac gaggcgctgt ggggcaaggc cctgatgctg
     1621 gtcggagtca tcatcaccct ctccgcggtg atcggctggg gcatggagcc gctcgaagaa
     1681 ccgcgtccgt cgtcggcggg agcccactag tgaccaccgc cgcccacgat gaggccagcg
     1741 ctgacggaca tggcgaccac acgcttgagg atgcgcatca tcccgagcac gagtcgaccg
     1801 gtgtcgagaa ccgcaagctg gcgatgtggg cgtttctcag ctccgagttc ttgttcttcg
     1861 gcgctctcat caccaactat ctcctctact cgtcaagaga cttcgcccag gggccgactc
     1921 cggcccagat ctacgacatc cccttcacct cggtcagctc gttcgtgctg ctcatgagct
     1981 ctctgaccat ggtgctggcc cacaatgcag tgagccgcgg cgaccagggc gctacccgaa
     2041 cctggctcct ggcaacggcc atgctcggat cggtcttcct ggcgggccag gtgttcgagt
     2101 tcacagagtt catcaccctc gaaggcatga cccttcgcac caccccggcg gcctctgcgt
     2161 tctacgtgct gaccgggttc cacggcaccc acgtcgccat cggcgtgatc atgctccttg
     2221 cgcttttcgg cttgtccaag cgtctcgggg gtctttccga caagcagttc ctcaacctgg
     2281 aattggtcgg tctgtactgg cacttcgtgg acatcatctg gatcgtgatc ttcactgtgg
     2341 tctatttgct caccgccgtt cccggaggcg ggtgacatga cccaggagca ccagatccat
     2401 caccccagcc cggtccaata cgtcaagatc gccatcggcc tggcggtcct caccgccatt
     2461 gaagtggcgc tgttctacat caacgagtcg cttcagctcg gatggatcaa caccgccgcc
     2521 ctgctgaccc tggctttcct caagttcgtc atcgtcatcg g
//
LOCUS       DATITW010000509         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_145245, whole genome shotgun sequence.
ACCESSION   DATITW010000509 DATITW010000000
VERSION     DATITW010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145245"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /locus_tag="VLS86_07080"
     CDS             complement(<1..1907)
                     /locus_tag="VLS86_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50295.1"
                     /translation="MAHRMTDASPPLIAGESTNEALTSTPKIKVVVTVVEGADLEAAL
                     ATVERQVYDSVEEVIVVGGSGAELPDGVGRSSTLEDAIATTPHDVEYLWILHSDARPR
                     PDALAALVSEMNRNEASLGGSKLLVAGTRDVLESVGSATDVFGEPFSGLDEGEIDLQQ
                     YDVVREVAFVAAVSMLVRRDLAQGLQGLDDLLEPGAAGLDFSQRVRLAGGRVITVPSS
                     EVYHQGRCRQAGRGWREQAGRLRAMLKAYKLITLAWVIPFLLFVGLVDSLFNLILLRW
                     RPAARHAASWGWNLLHLPSTITARRRFKPVRAFGDEELFRFQARGSVRMRDLGAELTD
                     RLLFMFDDDKALARETRRIWSSPGIWGAVIAAVLVVLAARSLFFTGVPNVGFSFDFEP
                     PSVALTRFFGGWNDSGLGSPGPVHPSTGISGLSSLLWFGAEGAARVLMTIGAAILAVL
                     GMGRLGGRLGLRGPGRYLSGLVLIAGPGTAVAIGAGSWLALGAASLVPWAVRSVFVHA
                     WEPAKGTLTRLAWLILWSVLLAAMSPVLVVVPLVTALLARALGDARSRVWLSFAGLIA
                     LIIAVPFVMGDPGWLLDVDRRLGLGVAELWPVLLTVAVLPVLFGEDGIGRLGLVGAIL
                     SLSSLLLVRVAL"
     gene            1950..>2314
                     /locus_tag="VLS86_07085"
     CDS             1950..>2314
                     /locus_tag="VLS86_07085"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HSO50296.1"
                     /translation="MNVVELSGGVGGARLARGLAAIEDLDLTLIVNVGDDETIHGLRV
                     SPDIDTVIYTLAGVEGPEGWGSLGDTLAVNDELGRLGMDNRFRLGDLDLALNLYRTHR
                     LAAGEPLARITDNIREHFG"
BASE COUNT          499 a          763 c          731 g          321 t
ORIGIN      
        1 agggctaccc gtaccaacaa caaggaggag aggctgagaa tcgcacccac caacccaagt
       61 cgcccgatgc catcctcccc gaacaagacc gggaggacgg ccacggtcaa cagcaccggc
      121 cacagctccg caacaccgag accgaggcgc cggtcgacgt cgagaagcca tcccggatct
      181 cccatcacga agggcacagc gatgatcagg gcgatcaggc cggcgaatga cagccagacc
      241 cgggacctgg catcgccgag agctcgcgcc aggagagccg tgaccagggg gacgaccacc
      301 aagacagggg acatcgcagc caacagcacc gaccagagga ttagccaggc caggcgagtc
      361 agagtgcctt tggccggttc ccaggcatga acgaagaccg agcggacggc ccacggaacc
      421 agggaagccg cccccagcgc caaccaagag ccggcaccga tcgccactgc cgtgcccggc
      481 ccggcaatca acaccagtcc ggacaggtac cgaccagggc ctctcagtcc aagacggccc
      541 ccaagtcggc ccatacccag aaccgccaga attgcggctc cgatcgtcat cagcaccctg
      601 gcggcgcctt ctgcgccaaa ccagaggagc gatgacaggc cgctgatccc cgtcgacgga
      661 tgcaccggac ccggagagcc gagaccggag tcattccagc ccccgaagaa ccgggtcaag
      721 gcgaccgacg gtggttcgaa gtcgaaactg aaaccgacgt tgggcacacc ggtgaagaag
      781 agggaccgtg cggcgagaac caccagcacc gcggcgatca ccgcgcccca gatcccgggg
      841 ctggaccaga ttctccgggt ttcgcgggcc agggccttgt cgtcgtcgaa catgaagagg
      901 aggcggtcgg tgagctcggc cccgagatca cgcattcgaa ccgaccctcg ggcttgaaag
      961 cgaaacaact cctcgtcccc gaaggcccgt accggtttga accggcgccg agcggtgatg
     1021 gtcgagggca ggtgaagaag gttccagccc cacgaggcgg catgacgggc cgccggtctc
     1081 cagcgaagga ggatcaggtt gaacagggaa tcgaccaggc ccacgaagag cagaaacggg
     1141 atcacccagg ccagggtgat gagcttgtag gccttgagca tggcccggag ccgcccggct
     1201 tgttccctcc aaccgcggcc cgcctgacgg caccgcccct ggtgatagac ctctgaagag
     1261 ggaaccgtga tcacacgacc cccggccaac cgaacccgtt gtgaaaagtc gagacccgcc
     1321 gctcccggct cgagaagatc gtcgagcccc tgcaaaccct gggccagatc gcgtcggacc
     1381 aacatcgaca ccgcggccac gaacgcgacc tcacgcacaa cgtcgtattg ctggaggtcg
     1441 atctcgcctt cgtccaaacc ggagaagggc tcgccgaaca cgtcggtggc gcttccaacc
     1501 gattccaaca cgtccctggt gcccgccacc aggagtttcg agccaccaag cgaggcttcg
     1561 ttgcgattca tctccgacac cagagccgcc agggcatcgg gacgcggacg ggcatcggag
     1621 tggaggatcc agaggtattc gacatcatgg ggggtggtag cgatggcatc ctcgagtgtc
     1681 gaagagcgcc ctactccatc gggcagctca gctccagacc cgcccacaac gatgacctct
     1741 tcgactgagt catacacttg cctctcgaca gtggccagcg ctgcttccaa atccgcgcct
     1801 tcgacgacgg tgaccaccac cttgatcttg ggcgtgctcg taagcgcttc gtttgttgat
     1861 tctcccgcga tcagcggagg cgaggcatcg gtcatgcggt gcgccattgt gaaccgggtc
     1921 ggggttctga gcaatattgg acaccaacgg tgaacgtggt ggagctctcc ggcggggtag
     1981 gtggggcccg tttggcgcgc gggttggctg ccatagagga tctggatctg acgctcatcg
     2041 tcaacgtcgg cgacgacgag acaatccacg ggctcagggt gtcaccggac atcgacacgg
     2101 tcatctacac cctcgccggc gtcgaggggc cggaggggtg gggtagcctc ggggacacgc
     2161 tcgccgtgaa cgacgaactg ggccggctcg ggatggacaa tcggttccgg ctgggcgatc
     2221 tcgatctcgc gctcaatctc tatcggacac atcggctcgc cgccggggag ccgctggcgc
     2281 ggataacgga caacatccgg gaacatttcg gaat
//
LOCUS       DATITW010000510         2037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_626182, whole genome shotgun sequence.
ACCESSION   DATITW010000510 DATITW010000000
VERSION     DATITW010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626182"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VLS86_07090"
     CDS             <1..250
                     /locus_tag="VLS86_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HSO50297.1"
                     /translation="ACMGAGTCAQIAPEVFQQRHDGTWGVKEDPRYFESGVLFDGLTG
                     DEHGPDGHEGKARVPAPLLESVIEAAEECPAECIFVEA"
     gene            252..776
                     /locus_tag="VLS86_07095"
     CDS             252..776
                     /locus_tag="VLS86_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50298.1"
                     /translation="MTGREQPSGKRRQSLSVSVVLGIVLVPLAAFAASVLVDSEEPSE
                     TSSTTVPAAVAATQPEAVPSAVTASADDLAAACGDAGLVLVDAEADGSISEVQQAALD
                     GLREVCDQQGMPLPGKPAPEPIVQTVVVAGNASSNAAAQPNDVFEVEDDDRDDDDRDD
                     DHEEDDHDEDEDDD"
     gene            773..1102
                     /locus_tag="VLS86_07100"
     CDS             773..1102
                     /locus_tag="VLS86_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50299.1"
                     /translation="MSRLSPQSITGLVVLAAGLLVVLEVTTGAPEPGPEPEPVVVVAP
                     EVAVPEAPPASVPGVPPEVQRVLFASGNAERLGLDQLTELPPEVARVLIDYGMTLTIA
                     TNRGGEG"
     gene            1099..1431
                     /locus_tag="VLS86_07105"
     CDS             1099..1431
                     /locus_tag="VLS86_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50300.1"
                     /translation="MSRFDGARWNRILVWTGAALAWGTAFIATWLEPARDVDAAEPPP
                     AVGTPVEPATRATMPSPSPQGLVILRFTPVDAPPAQVLTVRVQGPSGPPAAVASSQPA
                     PAPESSGS"
     gene            1428..>2037
                     /locus_tag="VLS86_07110"
     CDS             1428..>2037
                     /locus_tag="VLS86_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HSO50301.1"
                     /translation="MIHLSFRAMGTNVEAWCPDDTAGERVRSWFEEVEQVCSRFRSDS
                     ELSRVNRSTDREVSVSRILAEVIRAGEWARDSTDGLVDIGVGSSVVGWGYDRTFEQVT
                     SLPNQPVATPAPEWDLRSRVLRRSPHTILDLGGIAKGWACDRAVELGMASVVSAGGDI
                     RSVDSGTAVPVVDPWGEMTARVELGVGGLATSSTARRRWMVGS"
BASE COUNT          328 a          656 c          715 g          338 t
ORIGIN      
        1 cgcctgcatg ggtgccggca cctgtgctca gatcgcccct gaagtgttcc agcaacgtca
       61 cgacggcacc tggggcgtaa aggaggatcc tcgctatttc gaaagtgggg ttctcttcga
      121 cgggttgacc ggcgacgagc acggccctga cggacatgag ggaaaggcca gggttcccgc
      181 cccactgctc gagtcggtca tcgaggccgc agaggagtgc ccggccgagt gcatattcgt
      241 ggaggcatga gatgaccgga cgtgaacaac cctcgggaaa gcggcggcag agcctgtcgg
      301 tctctgtcgt ccttggcatc gttcttgtcc cgctggccgc attcgccgcc tcggtgctgg
      361 ttgacagcga ggagccaagt gagacgtcct cgacgacggt cccggcggcg gttgccgcca
      421 cccagcccga agccgtgccc tctgcagtga cagcgagtgc cgacgatctc gcggcagcct
      481 gtggtgacgc ggggttggtc ctggtcgacg ccgaagcaga cggctcgatc agtgaagttc
      541 agcaggcggc tctcgacggg ctgcgcgagg tgtgcgacca gcagggaatg ccgcttccgg
      601 gaaaacccgc tcccgagccc atcgttcaga cggtcgtggt cgccggcaac gcatcctcga
      661 acgctgccgc ccagccgaac gacgtcttcg aggtcgaaga cgacgaccgc gatgacgacg
      721 accgcgatga cgaccacgag gaggacgacc acgacgagga cgaggacgac gattgagccg
      781 cctctcaccg cagtcgatca ccgggctcgt cgttctcgct gccggattgc tggttgtcct
      841 agaagtgacc accggcgccc ctgagcccgg gcccgagccc gaacctgtgg ttgtggtcgc
      901 tcccgaggtg gcggtccccg aggcgccgcc tgcgagtgtg ccgggcgtgc ctcccgaggt
      961 gcagcgtgtc ctgttcgcca gtggcaacgc cgagaggctc ggcctcgacc agctgaccga
     1021 gcttcctccg gaggtagccc gggtcctgat cgactacggg atgaccctca ccatcgcaac
     1081 caatcgaggg ggagaaggat gagccgcttc gacggcgcgc ggtggaatcg gatcctggtt
     1141 tggaccggag ccgccctcgc ctggggcact gcattcatcg ccacctggct ggagccggcg
     1201 cgtgatgtcg acgccgcgga gcctccaccc gcagtcggga ccccggtcga gcccgcgact
     1261 cgggcgacca tgcccagccc ttccccgcag ggtctggtca tcctccgctt cacgcccgtg
     1321 gacgctccac ccgcccaggt cctcacggtc cgggttcagg ggccctccgg ccctccagct
     1381 gcagtggcct cctctcagcc ggcgccggcg ccggaaagca gcggatcttg attcacctca
     1441 gtttcagggc aatgggaacc aacgtcgagg cctggtgtcc cgatgacacg gccggcgaaa
     1501 gagtgagatc gtggttcgag gaagtggaac aggtgtgcag ccggtttcgc tcggacagtg
     1561 agctgagccg ggtcaaccgt tccactgacc gcgaggtgag cgtgtccagg atactggcgg
     1621 aggtcatccg agccggggag tgggcccggg actcgaccga cggtctggtc gacatcggcg
     1681 tgggctcgag tgtggtcggc tggggctatg accggacctt cgagcaggtg acatctctcc
     1741 cgaatcagcc ggtggcgaca ccggcgccgg agtgggatct ccgatcgagg gtcctcaggc
     1801 gctcgcccca cacgatcctg gatctgggtg gaatcgccaa gggatgggcc tgcgatcggg
     1861 ccgtcgagct gggcatggcg tctgtggtgt ccgccggggg ggatatcaga agtgtcgact
     1921 ccgggaccgc tgtccccgtg gtcgatccct ggggcgagat gacggcccgg gtcgagctcg
     1981 gagtgggggg tctcgctacc agttcgactg ctcgacgccg gtggatggtc gggtcac
//
LOCUS       DATITW010000511         1902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_305521, whole genome shotgun sequence.
ACCESSION   DATITW010000511 DATITW010000000
VERSION     DATITW010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305521"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VLS86_07115"
     CDS             <1..920
                     /locus_tag="VLS86_07115"
                     /inference="COORDINATES: protein motif:HMM:NF006091.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HSO50302.1"
                     /translation="VDFKDLLDKHVVIYGQSQLQKDLYDAVEAAEITLIDEAHDLRLH
                     DIKGKSPGVTFTRHGQTHTLLSDFIAGCDGAHSVTRDYIPPSAGKSFERTYPFVWVGV
                     LSETPPVSDEIIYANHETGFALCSMRNENLSRYYVQGSAEDLLDDWPDDRFWETLMQR
                     LPDSVSDRLVTGPSIEKLVTPLRSWVSEPMRRGRLFLAGDAAHVVPPTGAKGLNLAIS
                     DVVYLAQAFADHYATGSTEALDAYSDTALSRVWQAVRFSWWMTAMMHRFPGVSDDFDR
                     RMQLTELEYLERSETARRSLAENYVGLPL"
     gene            947..1306
                     /gene="pcaC"
                     /locus_tag="VLS86_07120"
     CDS             947..1306
                     /gene="pcaC"
                     /locus_tag="VLS86_07120"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HSO50303.1"
                     /translation="MEDGMEVRRRVLGQEHVDRATAATTDFDRSFQEWITESVWGGIW
                     TRPGIDIRSRSMVTIAILGALNHEELELHLRATRNTGLTPEEVAEVLLHVAAYAGVPA
                     GNRAFKLAKAIYEEDAR"
     gene            1303..>1902
                     /locus_tag="VLS86_07125"
     CDS             1303..>1902
                     /locus_tag="VLS86_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HSO50304.1"
                     /translation="MTEPVIRHYGPRAYSDHPPFRYPDYKSTVKRSPSHDLLSIVATL
                     SETTGPGPIWSDISEEDADLTMNAGTGSPAIGERIIVTGHVLDENGNGVPGTILEIWQ
                     ANACGRYAHWRETAFPAPLDPNFIGVGQCRTSETGEYRFTTIKPGPYPWGNHPNAWRP
                     AHIHFSVLGPAQLTRLVTQMYFEGDPLFPLDPIYNSAPEQ"
BASE COUNT          375 a          646 c          583 g          298 t
ORIGIN      
        1 gggtcgattt caaagacctg ctcgacaagc acgtcgtcat ctacggccag agccaattgc
       61 agaaggatct atacgacgcg gtcgaggccg ccgagatcac cctgatcgac gaagcacacg
      121 acttgcgtct tcacgacatc aaaggcaaga gccccggggt cacgttcaca cggcacgggc
      181 aaacccatac cctcctatcc gatttcatcg ccggatgcga cggggcgcac agcgtgaccc
      241 gcgactacat accgccctcg gcggggaaga gcttcgagcg aacctatccc ttcgtctggg
      301 tgggggtcct cagcgagacg ccgccggtca gcgacgagat catctacgcc aaccacgaga
      361 ccgggttcgc cctgtgctcc atgcggaacg agaacttgag ccggtactac gtgcagggct
      421 ccgcagagga cctgctcgac gactggcctg acgacaggtt ctgggagacc ctgatgcaga
      481 gactgcccga cagcgtgtct gaccgactgg tgaccggccc ctcgatcgag aagctggtca
      541 caccgctgcg cagctgggtg tccgagccga tgaggcgagg acggctgttc ctcgccgggg
      601 acgcagccca cgtcgtcccc cccaccggcg ccaaggggct caatctggcg atatccgatg
      661 tcgtctacct ggcccaggcg ttcgctgacc actacgccac aggctccacc gaagccctgg
      721 atgcctactc ggacacggct ctgtcccggg tgtggcaggc cgtgcgcttc tcctggtgga
      781 tgacagccat gatgcaccgg ttccccggag tcagcgacga cttcgaccgg aggatgcagc
      841 tgaccgagct cgagtacctg gagaggtctg agacggcgcg acgatccctg gcagagaact
      901 acgtgggtct gcccttgtga gcgaggagga aacgaccatg gtctcgatgg aagatgggat
      961 ggaggtgcgg cgccgagtgc taggccaaga gcacgtggac cgggccacag ccgccaccac
     1021 ggacttcgac cggagctttc aggagtggat caccgagagc gtctggggtg gcatatggac
     1081 gcggcccggc atcgacatcc gcagccggag catggtcacc atcgccatcc tcggggccct
     1141 caaccacgag gagcttgagc tgcatctgcg ggccacccgc aacaccgggc tcactcccga
     1201 ggaggtggcg gaagtgttgc tccacgtcgc cgcatacgcc ggcgtccccg ccgggaacag
     1261 agctttcaaa ctggccaaag cgatctatga ggaggacgcg cgatgaccga gccggtgatt
     1321 cggcactacg gccccagggc ctactccgac caccccccat tccggtatcc cgattacaag
     1381 tcgacggtga aacgaagtcc cagccacgac ctgctctcca tcgtggccac cctctccgag
     1441 accaccgggc ccgggccgat ctggtccgac atctccgagg aggacgccga cttgactatg
     1501 aatgccggga ccgggagccc ggcaatcggt gagcggatca tcgtcaccgg gcacgtcctc
     1561 gacgagaacg gtaacggggt gccggggacg atcctcgaga tctggcaggc gaatgcctgc
     1621 ggacgctatg cccattggcg cgagaccgca ttccccgccc ccctcgaccc gaacttcatc
     1681 ggcgttggcc agtgccgcac cagcgagacc ggcgaatacc gcttcaccac gatcaaaccg
     1741 gggccatacc cctggggcaa ccacccgaat gcctggcggc cggcacacat ccacttttcc
     1801 gtcctcgggc ctgctcagct gacacgcctc gtcacccaga tgtacttcga gggggaccca
     1861 ctcttccctt tggacccgat ctacaactcg gcgcccgagc ag
//
LOCUS       DATITW010000512         1653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_746185, whole genome shotgun sequence.
ACCESSION   DATITW010000512 DATITW010000000
VERSION     DATITW010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746185"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VLS86_07130"
     CDS             complement(<1..633)
                     /locus_tag="VLS86_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSO50305.1"
                     /translation="MNAVDFRTCPLCEATCGLEVTHRDQKVVRIRGDRDDVFSHGFIC
                     PKGSTLGKLDEDPDRLRSPLIRRDGLHQPATWDEAFQVIEDRLVPIIAEHGNDAVGVY
                     LGNPNVHSMSGVLYTRVFLKMLRTRSIFSAATVDQMPKHVSSGYMFGHPDLIPVPDID
                     RTSYLLMLGANPYESNGSLATAPDWPGRLRAIQERGGKVVVVDPRRTRTAA"
     gene            complement(630..1118)
                     /locus_tag="VLS86_07135"
     CDS             complement(630..1118)
                     /locus_tag="VLS86_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSO50306.1"
                     /translation="MSRRSLQHLECAVANTVEMVNDAWTVLILRDAFLGVRRFDEFVT
                     DLGIARNVLTDRLERLVANGMLYTSQYQDRPPRHEYRLTEQGKDLFDLLMALWSYGER
                     WKPPSDPGRQKVIHVTCGHEAQAVAHCGNCGQRLSRRDVRIEPALPVVGDRAVEAAGV
                     GS"
     gene            complement(1339..>1653)
                     /locus_tag="VLS86_07140"
     CDS             complement(1339..>1653)
                     /locus_tag="VLS86_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HSO50307.1"
                     /translation="EEIAVGPWFQFHHDEFTLPQGATGLARNESGLQAFRIGRSLAVQ
                     FHPEMTAELIASWCDEGGIEELSAAGVDHEQLIEESRLRAIESQPALERMLDWWLDEI
                     AG"
BASE COUNT          279 a          542 c          521 g          311 t
ORIGIN      
        1 ggccgcggtc cgtgtcctgc gggggtcgac caccaccacc ttgcctcccc gctcctggat
       61 cgcgcgcaga cgacccggcc agtcgggagc ggtagccagg cttccgttgg actcatacgg
      121 gttggcgccg agcatcagca ggtacgaggt acggtcgatg tccggaaccg ggatgaggtc
      181 cggatggccg aacatgtagc cggacgagac gtgctttggc atctggtcga cggtggccgc
      241 gctgaagatg ctcctggtgc gaagcatctt caggaacacc cgggtgtaga ggacgcccga
      301 catcgaatgg acgttggggt tgcccagata gacgccgacc gcgtcgttcc catgctcggc
      361 gatgatcggg accagcctgt cttcgatcac ctggaacgcc tcgtcccagg tggcgggctg
      421 gtgtaggccg tcgcgtctga tcaacgggct gcgaagccga tccgggtcct cgtcgagttt
      481 gcccagggtc gatcccttgg ggcagatgaa gccatggctg aagacgtcgt cgcgatcacc
      541 acggatccgc accaccttct ggtcgcgatg ggtgacttcg aggccacagg tggcctcgca
      601 cagcgggcag gtcctgaagt cgaccgcgtt caacttccca cccccgctgc ctcgacggcc
      661 cgatcgccga caacggggag cgccggctcg atccggacgt cacgcctgct cagccgctgg
      721 ccacaattcc cgcagtgggc cacggcctgt gcctcgtggc cacaggtgac atggatcacc
      781 ttctgtctcc cggggtcgga tggcggcttc caccgctccc cgtagctcca gagcgccatc
      841 agcaggtcga acaggtcctt gccctgctcg gtgagccggt actcatgccg gggcgggcgg
      901 tcttgatact ggctggtgta caacatgccg ttggccacca gccgctcgag tcggtcggtg
      961 agcacgttgc gtgcgatccc gaggtcggtg acgaactcgt cgaatctcct caccccgaga
     1021 aatgcgtccc tgaggatgag gacggtccag gcgtcgttga ccatctccac cgtgttggcc
     1081 acggcgcact cgaggtgttg cagtgatctt ctcgacatga ccacgatcat agcgagtagt
     1141 tagttgcgat gtgctactca ttgtggctag agtcagtagt cattcgaaac tcaggagaaa
     1201 gagaatgcgt tttcgccaca tccggagacg gcgacgtcag ccaagtcctc cgttcttcct
     1261 gctcccgctc gacatcgagc tcggcgagat catggcggac ctggtgttcc cggctccggc
     1321 ctagctgaac ggctcggatc aacccgcgat ctcgtccagc caccagtcga gcattcgctc
     1381 caaggccggc tgcgattcga tggctcggag ccggctctcc tcgatcagct gctcgtgatc
     1441 gactcccgcc gcgctcaatt cctcgatgcc accctcgtca caccacgagg cgatcagctc
     1501 ggccgtcatc tccgggtgga actgcaccgc gaggctccgc ccgatccgga aggcctggag
     1561 ccccgactcg tttcgggcca gcccggtggc gccttggggc aaagtgaact cgtcgtggtg
     1621 aaactggaac cagggcccga cagcgatctc ctc
//
LOCUS       DATITW010000513         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_385670, whole genome shotgun sequence.
ACCESSION   DATITW010000513 DATITW010000000
VERSION     DATITW010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385670"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VLS86_07145"
     CDS             complement(<1..408)
                     /locus_tag="VLS86_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50308.1"
                     /translation="MSGGPLRVLEIYGDPEDFGRTHGTECAPLIGAFLADRLGLSGDA
                     TWAGRSADAGLVLSLAEETLPFHRSYSPSLYAEMEAMAAAAGITPAEAVVVGGFTDLV
                     DVVRAHTGDAPTEDDCTAVLNPGQKVLAQTWDMH"
     gene            491..1480
                     /locus_tag="VLS86_07150"
     CDS             491..1480
                     /locus_tag="VLS86_07150"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HSO50309.1"
                     /translation="MKKLTLILALGLVLAACAGDDTGETFSEVSSGLDGGFTEGDDGG
                     AFVTTVAASGEAPEPGEFPDEEAAPERQVIRQASLELEADDTRSAFEEIVALVEGVGG
                     FVSNATVHPVVNEDDQPEVSMVLRIPADELSSVMTQIKGMVEEVVTESQDAQDVTAEF
                     VDLEARLTNLQALEVELVALLAEVREQPEADPAKLLLVFNEVSRVRGEIEQLQGQLNY
                     LNDVVALATLSVYLAPTPAAVPIVEEGWAPLEVARDSLRSLVSGLQDVAEWGINFALY
                     TLPLLLLILGIPLLILWLVFRRLRKRRPPAAAPPDAVPPAPIPPVIPVKTTDE"
BASE COUNT          287 a          487 c          495 g          231 t
ORIGIN      
        1 atgcatgtcc caggtctggg ccagcacctt ctgcccggga ttgaggacgg cggtgcaatc
       61 gtcctcggtg ggggcatccc cggtgtgggc ccggaccacg tcgaccaggt cggtgaagcc
      121 accgacgacc accgcctcgg ccggggtgat cccggcggcg gcagccatgg cttccatctc
      181 cgcgtagaga ctcggcgaat aggagcgatg aaagggaagg gtctcctcgg cgagagacaa
      241 caccaggccg gcgtcggccg atcttcccgc ccaggtggca tcacccgaca acccgagccg
      301 gtcggcgagg aacgccccga tcagaggggc gcactcagta ccgtgagtcc gaccgaaatc
      361 ctccggatca ccgtatatct cgaggacgcg aagcggcccg ccactcatga ccccacggta
      421 cccgggaacc gaacctcgcg atatgtgcgt ccaacggcca agcccacggg ggcacacgag
      481 aggacaaatc atgaaaaaac tgacactgat cctggccttg ggactggtgc tcgccgcctg
      541 cgccggtgat gacaccggag agaccttctc cgaagtcagc tcggggctgg acggcggctt
      601 caccgaagga gatgacgggg gagccttcgt caccacggtg gcagccagcg gggaggcacc
      661 ggagccggga gagttccccg acgaggaagc ggctccggaa cggcaggtga tccgtcaggc
      721 cagcctcgaa ctggaagcag acgacacccg gtccgccttc gaggagatcg tcgccctggt
      781 cgaaggggtg ggcgggttcg tgtcgaacgc cacggttcac cccgtggtca acgaggacga
      841 ccagcccgag gtgagcatgg tgctcaggat ccccgccgat gagctgtcgt cggtgatgac
      901 ccagatcaag ggcatggtcg aagaggtcgt caccgagtca caggatgctc aggatgtgac
      961 tgctgagttc gtggatctcg aagccagact caccaacctc caggcgctcg aagtcgagtt
     1021 ggtggccctg ctcgccgaag tcagggagca gcccgaagcc gatccggcca agctgcttct
     1081 ggtcttcaac gaggtctccc gggtccgtgg cgagatcgag caactccagg gacagctcaa
     1141 ctacctcaac gacgtggtgg ccctggcgac cctgtcggtc tacctggccc ccaccccggc
     1201 cgcggtgccg atcgtcgaag agggatgggc tcccctcgaa gtggcccgtg actcgctgcg
     1261 gagcctggtc agcggtctgc aggatgtcgc agagtggggg atcaacttcg ccctgtacac
     1321 cctgcctctg ttgttgctga tcctggggat cccgctgctg atcctgtggc tcgtgtttcg
     1381 acggttgcgg aagagacggc ccccggccgc agcacccccg gacgcagtac ccccggcacc
     1441 gatcccgccg gtcatccccg tcaagacaac cgacgagtaa accgacgagt gaacagtgtg
//
LOCUS       DATITW010000514         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_706636, whole genome shotgun sequence.
ACCESSION   DATITW010000514 DATITW010000000
VERSION     DATITW010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706636"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VLS86_07155"
     CDS             complement(<1..823)
                     /locus_tag="VLS86_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSO50310.1"
                     /translation="MRTATRDERLQRRQVGVLLAAGTAVISGIAIFVNGYGVRAWTEI
                     SDATTYTTLKNSVAAVVLLVGSGLLARKSRAPREEMAGVSRLTPGLVLIAVVGGSIPF
                     VLFFEGLARATSADAAFIHKTLMVWVTFLAVLVLREKIGALHIAAIAMLIGGQVALSG
                     GIGAIEFGAGEWMILAATLLWAVETVVAKRVLVSVPWHTVGAARMAGGAALLVLYGVG
                     RGGFAGLTGVTTEHVVWILLTGRTLSAYVATWFAALSRAPAVDVTAVLAGGAIITA"
     gene            930..2039
                     /locus_tag="VLS86_07160"
     CDS             930..2039
                     /locus_tag="VLS86_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSO50311.1"
                     /translation="MVVDRDDIDILFDVLAADGYTVMGPTVRDRAIVYDHIQGVDDLP
                     EGLTDEQDGGEYRLLPREDRALFGYAVGQQSWKRFLFPPRTTLFQVDQSNDSLRFVES
                     DAPAVRYAFLGVRACELAAIAIQDRVFLGSGVADRTYDSARQGVFTVAINCMVAGGTC
                     FCVSMGTGPRCESGYDLVLTEVIDHDRHEFVFEAGSEAGESVLARLQGRDASETDRMS
                     VDRIVEHTAASMGRSMETTGIRELLLGNLEHPRWEDVAQRCLACTNCTLVCPTCFCST
                     TEDTVTLDGALATRSRRWDSCFTMDFSSLHGVAVRSSTKSRYRQWMTHKLATWIDQFG
                     SSGCVGCGRCITWCPVGIDITEEVAAIRATQEVVV"
     gene            2036..2491
                     /locus_tag="VLS86_07165"
     CDS             2036..2491
                     /locus_tag="VLS86_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HSO50312.1"
                     /translation="MRLGPIRDSSELLTRLSNTELELFETISREVHLSTGEILFEEGG
                     QADTFYIIVSGKVGLELTSPGRVPMVLQTLGDGDLVGVSWFFPPYQWNWRARALEDTN
                     LVAFDAVEVRRRCEINRDLALEVLSVVAEEAVRRLHGTRIQLLDLYRNS"
     gene            2495..>2792
                     /locus_tag="VLS86_07170"
     CDS             2495..>2792
                     /locus_tag="VLS86_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50313.1"
                     /translation="MTSVADLVDSGSRAEAMLPRPFRVIAHRAETPDTVTLELESEDG
                     EGMSFSPGQFTMLYVFGIGEVPISISGDPHRPQTLVHTVRSVGAVTAAIAGLRPG"
BASE COUNT          494 a          931 c          914 g          453 t
ORIGIN      
        1 gagcggtgat gatggccccg ccggccagca cggccgtgac gtccacggcc ggggcgcgag
       61 acaaggcggc gaaccaggtc gcgacatagg cggacaaggt ccgaccggtg aggaggatcc
      121 aaaccacatg ctcggttgtg acgccggtga gcccggcgaa accacctcgt cccactccgt
      181 agaggacgag gagcgccgcc cctcccgcca tgcgggccgc gccaaccgtg tgccatggca
      241 ccgagacgag gacgcgcttg gcgacgacgg tctcgacggc ccacagcaga gtggccgcca
      301 ggatcatcca ttcccctgcc ccgaactcga tggcgccgat gccaccggac aacgcaacct
      361 gaccgccgat cagcatggcg atggccgcga tgtggagggc accgatcttc tcccggagca
      421 ccagcaccgc caggaacgtg acccacacca tcagcgtctt gtggatgaac gcggcgtcgg
      481 ccgaggtggc gcgggccagt ccctcgaaga acaggacgaa gggaatgctg cccccgacga
      541 ccgcgatgag caccaggccc ggcgtcaatc tcgagacgcc cgccatctcc tctcggggcg
      601 ccctgctctt ccgggcgagc aagcctgacc ccacgaggag caccaccgca gcgaccgagt
      661 tcttcaacgt ggtgtaggtc gtggcgtccg agatctcggt ccaggcccgc accccataac
      721 cgttcacgaa gatggcgatg ccgctgatca cagcggttcc cgcggccaac aggacgccca
      781 cctgtctccg ctgcagccgt tcgtctctcg tcgcggtgcg cacggctccg accctaccgg
      841 acgctcggag cccgccgccg caccgggcag ccatcaaccg aaacaccaaa tcggtactgt
      901 cgctcgggaa tggaggtcat gtgagccaga tggtcgtcga ccgtgacgac atcgacatcc
      961 tcttcgacgt cctggcggcc gacggttaca cggtcatggg gccgaccgtc cgcgaccggg
     1021 cgatcgtcta cgaccacatc caaggcgtcg acgacctgcc cgaggggctg accgacgagc
     1081 aggatggtgg ggaataccgt ctgctgccca gagaggaccg ggcgctgttt ggctatgcgg
     1141 tcggccagca gtcgtggaag aggttcctct tccccccgag aacgaccctg tttcaggtgg
     1201 accagagcaa cgactcgctc cggttcgtcg agagcgacgc ccccgcggtg cgctacgcct
     1261 tcctcggggt gcgtgcctgc gagctggccg ccatcgccat ccaggaccgg gtgttcctgg
     1321 gcagcggagt ggccgaccgg acctacgact cggcacgcca gggtgtgttc accgtcgcca
     1381 tcaactgcat ggtggccggt ggcacctgtt tctgcgtgtc gatgggaacc gggccccgat
     1441 gtgagtccgg atacgacctg gtcctgaccg aggtcatcga ccatgaccgg catgagttcg
     1501 tcttcgaggc gggcagtgag gccggagagt cggtgttggc gagactccag ggccgggacg
     1561 cctccgagac cgacaggatg agtgtcgacc ggatcgtgga gcacaccgcg gcgagcatgg
     1621 gcaggtcgat ggagaccacc gggatcagag agctgcttct gggcaacctc gagcacccgc
     1681 gctgggagga tgtggcgcag aggtgcttgg cgtgcaccaa ctgcaccctg gtctgcccga
     1741 cgtgcttctg ctcgacgacc gaggacacag tgaccctgga cggagcactc gcgacccggt
     1801 cgcgacgatg ggactcgtgt ttcaccatgg acttctccag cctgcacggg gtcgccgtcc
     1861 gcagctcgac caagtcgagg tatcggcaat ggatgactca caagctggcg acctggatcg
     1921 accagttcgg gtcctcgggg tgtgtcggtt gcggccggtg catcacctgg tgcccggtcg
     1981 gcatcgacat caccgaggag gtcgccgcga ttcgcgccac ccaggaagtt gtcgtatgag
     2041 actcggaccg attcgtgaca gcagcgaatt gctcacgagg ctctccaaca ccgagctcga
     2101 gctcttcgag acgatctcac gagaggtcca cctgagcaca ggtgagatcc tcttcgagga
     2161 ggggggccag gccgatacct tctacatcat cgtctccggc aaggtcggtc tcgagctgac
     2221 cagcccgggc cgggtcccca tggtcctgca aacgctgggc gacggggacc tcgtcggcgt
     2281 cagctggttc tttcccccgt atcagtggaa ctggagggct cgcgccctcg aggacaccaa
     2341 cctggtggcc ttcgatgccg tcgaagtccg gcgtagatgc gagatcaacc gggacctggc
     2401 cctcgaggtg ctgagtgtcg tcgccgaaga ggctgtgcga cgtctgcacg gcaccaggat
     2461 ccaactcctg gatctctaca ggaacagctg accgatgacc tccgtggccg acttggttga
     2521 ctcggggtcg agagccgagg cgatgctgcc ccgtcccttt cgtgtgatcg cccaccgtgc
     2581 ggagacgccg gacaccgtca ctctggagct cgaatccgag gacggagaag gcatgtcctt
     2641 ctcccccgga cagttcacga tgctctacgt tttcggcatc ggcgaggtgc cgatctcgat
     2701 ctcgggtgat cctcaccgtc cccagaccct ggtccacacg gtgcgctcgg taggagcggt
     2761 gaccgcggcc atcgccggtc ttcgccccgg cg
//
LOCUS       DATITW010000515         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_25831, whole genome shotgun sequence.
ACCESSION   DATITW010000515 DATITW010000000
VERSION     DATITW010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25831"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VLS86_07175"
     CDS             complement(<1..662)
                     /locus_tag="VLS86_07175"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HSO50314.1"
                     /translation="MSSRRAPGRISFRAQLLVYFGIVYVALIAAMTSIIILSSDGSSL
                     ATVIGIGVATAVVGLAAIAWLARSIASPVAELTAQTKALARGQSDQELHRSPIREIDQ
                     LSLALAGLEEDNRLRLQEAGRASATLEVVLGALPQGTVLFDVDDNVVYANPSAHQLLG
                     AMPQNLSGLAPFAFQTAVRECREQRAQTSRVVEHGRPVRTMNGIATPFADDERVLLVI
                     LDV"
     gene            complement(649..1368)
                     /locus_tag="VLS86_07180"
     CDS             complement(649..1368)
                     /locus_tag="VLS86_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016443352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSO50315.1"
                     /translation="MTGPAENRRHRVLVVEDEPALAESVKYALEIEGFDVLVAETGVA
                     GLESARVNQPDLVLLDLMLPGMSGLDVCRQIRTSSDVPIIMLTAKDAESDKVAGLELG
                     ADDYMTKPFSMRELTARVRAQLRRAMKSGALSESNEVLRGGSIELDLDAHETRVDGAP
                     VELRPKEFELLESLMRRKNRLAARHTLIDEVWGSSYFGDTKTLDVHIKRLRQKLERDP
                     SAPSQIVTVRGLGYKFVDEQS"
     gene            complement(1352..2059)
                     /gene="phoU"
                     /locus_tag="VLS86_07185"
     CDS             complement(1352..2059)
                     /gene="phoU"
                     /locus_tag="VLS86_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HSO50316.1"
                     /translation="MTHAQDINLHFHQELEALEQTVLGVVDRAEQMVAMAVEAVTTGD
                     HALAEQVVELDNEIDRTYFTVHNDWVHLMARFQPLGSDLRKMSILLHLNNTFERMGDQ
                     CVNIAKITRFNEGLPRVERIAEQIQEMGDLVRPMIRAAIEAYLRQDLDEARLLPAMDQ
                     PVDRLNSNMYKEIVAVGGDPQQLEWATKMLMASRAWERIGDQAVDFAEQTIYLITGER
                     AEFNETGLDGSADDGSG"
     gene            complement(2056..2868)
                     /gene="pstB"
                     /locus_tag="VLS86_07190"
     CDS             complement(2056..2868)
                     /gene="pstB"
                     /locus_tag="VLS86_07190"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012361492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HSO50317.1"
                     /translation="MVTTRADQGPIDLPVVFSVRDLSVYYGDFRAVSNVEMPIRRNEI
                     TALIGPSGCGKSTVIRCFNRMNDLIESARVEGHVEYHGVDLYSDDVDPVEVRRRVGMV
                     FQKPNPFPKSIYDNISFGPKIGKYKGNMDDLVEQSLRKAALWDEVKDKLDESGMALSG
                     GQQQRLCIARAIATSPDVLLMDEPCSSLDPIATLKIEELMLELKDEYSIIIVTHNMQQ
                     AARVSDRTAFFNVRLSDEGQRTGYLVEFSPTEELFTSPREKDTEDYITGRFG"
BASE COUNT          556 a          996 c          944 g          610 t
ORIGIN      
        1 acgtcgagga tgacgaggag gaccctctcg tcgtcggcga acggggtggc gatgccgttc
       61 atcgtcctga ccggtctccc gtgctcgacc accctcgaag tctgcgcccg ctgctcacgg
      121 cactcgcgga ctgcggtctg aaaggcgaac ggcgccagac cggacagatt ctgcggcatg
      181 gccccgagca actggtgagc ggaggggttg gcgtacacca cgttgtcgtc cacgtcgaag
      241 aggaccgtgc cctgggggag ggctccgagc acgacttcga gggtggccga ggcgcggccc
      301 gcctcctgca atcgcagccg gttgtcctcc tcgagaccgg ccagggccag actgagctga
      361 tcgatctccc gtatcgggga ccgatgaagc tcctggtctg actgtcccct ggccagggcc
      421 ttggtctggg cggtgagctc ggcgacggga gaggcgatgc tccgcgccag ccaggcgatg
      481 gcggcgagac cgacgacggc cgtggcgaca cctatcccga tgacggtggc gagactcgat
      541 ccatccgagg acaggatgat gatcgaggtc attgccgcga tcagcgccac gtagacgatc
      601 ccgaaataga cgagaagctg cgcccggaac gagattctgc ccggggctct acgactgctc
      661 atcgacgaac ttgtaaccca gcccgcgcac ggtgacgatc tgtgacggag cggaggggtc
      721 ccgctccagc ttctgacgca agcgcttgat gtgcacatcc agggtcttgg tatccccgaa
      781 gtagctcgac ccccagactt cgtcgatcag ggtatgtctc gccgcaagtc ggttcttgcg
      841 acgcatcaac gactcgagca gctcgaactc cttggggcgg agctcgaccg gggcgccgtc
      901 gactctggtc tcgtgggcgt cgagatcgag ctcgatggaa ccgcctcgca gaacctcgtt
      961 tgactcggac agggcgcctg acttcattgc cctcctcagt tgggcacgca ctctcgccgt
     1021 gagctcgcgc atcgagaacg gcttggtcat gtagtcgtcg gcgcccaact ccagacccgc
     1081 caccttgtcc gactccgcat ccttggcggt cagcatgatg atcgggacgt cggacgaggt
     1141 tcggatctgg cggcagacgt cgagaccgga catcccgggc aacatcaggt cgagcaggac
     1201 gagatccggc tgattgaccc gggctgactc caacccggcc acaccggttt cggcaaccag
     1261 gacatcgaaa ccctcgatct cgagggcgta cttgaccgat tcggccaatg caggctcgtc
     1321 ctcgaccacg aggacacggt gtcgcctgtt ctcagccgga cccgtcatcg gccgatccgt
     1381 cgagccccgt ctcgttgaat tcagctctct ctccggtgat caggtagatc gtctgctccg
     1441 cgaagtccac cgcctgatcc ccgatcctct cccacgcccg agaggccatc agcatcttgg
     1501 tcgcccattc cagctgttgg ggatcacccc cgacggcgac gatctccttg tacatgttgg
     1561 agttgagtcg atcgaccggc tggtccatcg ccggcaggag cctggcctcg tcgagatcct
     1621 gacgcagata ggcctcgatc gcggccctga tcatgggccg caccagatcg cccatctctt
     1681 ggatctgctc cgcgatgcgc tcgacccggg gaagcccttc gttgaacctc gtgatcttgg
     1741 cgatgttgac gcactggtcg cccatccgct cgaaggtgtt gttgagatgg agcaagatgc
     1801 tcatcttccg caggtccgag cccaacggct ggaatcgtgc catgagatga acccagtcgt
     1861 tgtgcaccgt gaaataggtg cgatcgatct cattgtcgag ctcgaccacc tgctcggcca
     1921 gggcgtgatc accggtggtg acggcctcca cggccatcgc caccatctgc tcggcccggt
     1981 caaccactcc gagcacggtc tgctccagag cttcaagctc ttgatgaaag tgcaggttta
     2041 tgtcttgcgc gtgtgtcatc cgaaccggcc tgttatgtag tcctcggtgt ccttctcgcg
     2101 aggcgaggtg aacagctctt cggtcgggga gaactcgacg aggtagccgg tgcgctggcc
     2161 ctcgtcgctc agcctgacgt tgaagaacgc cgtcctgtcg ctcactctcg ccgcctgttg
     2221 catgttatgg gtgacgatga tgatcgagta ctcgtccttg agctccagca tcagctcttc
     2281 gatcttcaag gtagcgatcg gatccagcga tgaacagggc tcatccatca ggagcacatc
     2341 agggctggtg gctatggccc gtgcgatgca gagacgctgc tgctgccctc ccgagagcgc
     2401 catccccgac tcgtccagct tgtccttgac ctcgtcccac agggccgcct tgcgcaggct
     2461 ctgctcgacg aggtcgtcca tgttcccctt gtacttcccg atcttcggcc cgaaggagat
     2521 gttgtcgtag atcgacttgg ggaagggatt cggcttctgg aacaccatcc cgacccgtct
     2581 ccgaacctcg accgggtcca cgtcatcgga gtagagatcg acaccgtggt actccacgtg
     2641 accctcgacg cgggcgctct cgatcaggtc gttcatccgg ttgaagcacc ggatgactgt
     2701 cgacttgccg cagccggagg gaccgatgag agcggttatc tcgttgcggc ggatcggcat
     2761 ctccacgttg gacacggccc gaaaatcccc gtagtagacg ctcaggtctc gaacgctgaa
     2821 gacgaccggg aggtcgattg gcccctgatc tgctcttgtg gtgaccatct ctatcgccgg
     2881 ccggggccgg tcgagcaccc tctcctcggt ttgctgttcc aactcgtgct ccatgttaat
     2941 tagaggttct tctcgtaccg attgcgcaga tagaccgcgg tgaagttgat caggaacagg
     3001 acaaccagca tgacaacgat cagtgccgcg gtcagggcac gaaaaccttc ctgtgggaga
     3061 cgcgcccagg agaacaccag aaccggcatc gcgctgaacc gcccct
//
LOCUS       DATITW010000516         1826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_305884, whole genome shotgun sequence.
ACCESSION   DATITW010000516 DATITW010000000
VERSION     DATITW010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305884"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VLS86_07195"
     CDS             <1..537
                     /locus_tag="VLS86_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50318.1"
                     /translation="RLERLWWAPPAAPSQPFAVINLNFVYGVRTADRKLWLETAIQGC
                     DEAGVPYVISLHPAERARIDSNRVTSISASRLLPRASVLISRFSTMPLEAMARGVPFI
                     YHNPHRETVKTFADPMGAYDVSTTAEELAAAIRALDPIQHDPRTRSASFFSNHVDVDP
                     ARPSEIRAVEAIVEQLSS"
     gene            584..1504
                     /locus_tag="VLS86_07200"
     CDS             584..1504
                     /locus_tag="VLS86_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014913525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSO50319.1"
                     /translation="MSHESPDGSVDVSIVLPVFNEEGHLGDEIDRIRKTMDESRYSYE
                     ILVVDDGSTDGSGERLRQIDGIRLLQFATNRGSGSARKYGTQAARGQIVVWTDVDMTY
                     PNDTIPELVDQMEGFDQVVGARTSEEGTIKLVRKPAKWVIRRLASYLTGVRIPDLNSG
                     FRAFRRDVALPFLHLLPRGFSCVTTLTMSFLSNGYAIKYVPIEYAARAGESKFHWWKD
                     TKRYLIQVVRMTLMWEPMRVFGPPAAILGLVGVGKLIYDLVDKDFRVATNTVVILGVA
                     FALALIGMISDLMVQLNKRPQDVMPTTVDS"
     gene            complement(1522..>1826)
                     /locus_tag="VLS86_07205"
     CDS             complement(1522..>1826)
                     /locus_tag="VLS86_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HSO50320.1"
                     /translation="EIRTLDEFDLTPDILKIDVEGAEAAVVEGGMTTIESHLPVILLE
                     GDADQSERLLTELGYRRHRFDPDRNRMVAGESGTLNTFLLRPGHYRLFAGMEIVDS"
BASE COUNT          337 a          585 c          562 g          342 t
ORIGIN      
        1 cggctcgagc ggctgtggtg ggctccgccg gcggcaccca gccagccttt cgccgtgatc
       61 aacctcaatt tcgtctacgg ggtcaggacc gcagatcgca aattgtggct cgagaccgcg
      121 attcagggat gcgatgaggc cggagtgccc tacgtgatct cgctgcaccc tgccgagcgg
      181 gcacggatcg actcgaatcg ggtcacctcg atctccgcct cccgccttct cccccgggcg
      241 agcgtgctga tcagtcgctt cagcaccatg cccctggagg cgatggcgcg cggcgtgccc
      301 ttcatctacc acaacccgca ccgggaaacc gtgaagacct ttgccgaccc tatgggcgcc
      361 tacgacgtgt cgaccacggc cgaagagctg gcggcagcga tccgcgccct ggacccgatc
      421 cagcacgacc cgcggactcg ctcggcgtcg ttcttctcca atcacgtcga cgtcgacccg
      481 gctaggccaa gtgagatcag ggcggtggag gcgatcgtcg agcagctgag cagctgatgg
      541 gtgctgtcgg ggcccacgct gtcataatcg gctccgaatg acgatgagcc atgaatcgcc
      601 cgacggctcg gtcgacgtgt cgattgtcct gcccgttttc aacgaggagg gtcatctcgg
      661 cgacgagatc gatcgcatcc gcaagaccat ggacgaatcc cgttacagct acgagatcct
      721 ggttgtggac gacggatcga cggacggttc aggggagcgt ctgcgccaaa tcgacgggat
      781 ccgactcctg cagttcgcca ccaaccgtgg cagcggatcg gctcggaagt acgggaccca
      841 ggcggcacgc ggccagatag tggtctggac cgacgtcgac atgacctacc ccaacgacac
      901 catccctgag ctcgtggatc agatggaggg cttcgaccag gtagtcggag ccaggacgtc
      961 cgaggagggg accatcaagc tggtgcggaa gccggcgaaa tgggtgatac gccggctggc
     1021 ctcctatctc accggtgtca ggatccccga tctcaactcg ggatttcgcg ctttccgccg
     1081 agacgtcgca ctccccttcc tccatctcct accccgcggc ttttcctgtg tcacgactct
     1141 caccatgtcg ttcctgtcca acggttatgc aatcaagtac gtgccgatcg agtacgccgc
     1201 ccgagccggt gagtcgaaat tccattggtg gaaggacacc aagcgctatc tgatccaggt
     1261 ggtgcggatg accctgatgt gggagccaat gcgagtgttc gggccgccgg cggcgatcct
     1321 cggcctggtc ggtgtcggca agctgatcta cgacctggtc gacaaggact ttcgggtagc
     1381 gacgaacacc gtggtcattc tcggagtggc cttcgccctg gctctgatcg ggatgatctc
     1441 cgacctgatg gttcagctca acaaacggcc ccaggacgtg atgccgacca ccgtcgactc
     1501 ttagacgagc gagagtgcgt ctcaactgtc gacgatctcc atccctgcga acaagcgata
     1561 gtgaccgggc cgcagaagga acgtgttcaa ggtgcccgac tcgccggcca ccatgcggtt
     1621 tcgatcgggg tcgaatcggt gccttcggta cccgagctcg gtgagcagac gctctgactg
     1681 atcagcgtct ccctcgagca ggatcaccgg caaatgcgac tcgatggtcg tcatcccgcc
     1741 ttcgacgacc gcagcctcgg cgccttcgac gtcgatcttc aggatgtcag gggtcaggtc
     1801 gaactcgtcc agtgtgcgga tctcga
//
LOCUS       DATITW010000517         1763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_706834, whole genome shotgun sequence.
ACCESSION   DATITW010000517 DATITW010000000
VERSION     DATITW010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706834"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1763
                     /locus_tag="VLS86_07210"
                     /pseudo
     CDS             108..1763
                     /locus_tag="VLS86_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
BASE COUNT          327 a          573 c          547 g          316 t
ORIGIN      
        1 gtgacgatct tcaggcgctt gatcgccttc tgcttgcgct gcacgctcag cggcttcttg
       61 ccgtcctgcg ggtcgatcgc gtcgcgaagg cgctgctcca tcgtctcgat ctcgatgtcg
      121 agatggccac cctcaacgag cacctggaat cgggttcggc ccagaagcgg cagcgggcaa
      181 cccagcggat gaaggtcgtc aagccgttct gggagggcca gaaccgggcc gaggacatga
      241 tcctcgagac cgttccggtc atccccccgg atcttcgtcc catggtccaa ctcgacggtg
      301 gtcgtttcgc cacctccgac ctcaacgatc tctatcgccg ggtgatcaac cgcaacaacc
      361 gtctcaagcg cctgctcgac ctcggcgccc ccgagatcat cgtcaacaac gagaagcgga
      421 tgctccaaga ggcggtcgac gcactgttcg acaacggccg tcgcggccga gccgtgaccg
      481 gtcccgggaa ccgcgccctc aagtcgctct ccgacatgct caagggcaag caagggcgat
      541 tccgccagaa tctgttgggc aagcgtgtcg actattcggg gcgctcggtg atcgtggtcg
      601 gcccccagct caagctgcat cagtgcggcc tgcccaagat catggcgctg gagctgttca
      661 agccgttcgt gatgaagcgc ctggtcgacc tcgacctcgc ccagaacatc aaggctgcca
      721 agcgaatggt cgagcgccag cgtccccagg tctggaatgt cctctccgac gtgatccagg
      781 aacacccggt gcttctcaac cgggcaccca ccttgcaccg tctcgggatc caggctttcg
      841 agccggtcct ggtcgaaggc aaggcgatcc agatccaccc gttggtttgt gaggcgttca
      901 acgccgactt cgacggtgac cagatggctg ttcacctccc gctctctgcc gaggcccagg
      961 ccgaggccag ggtgctgatg ctgtcgtcga acaacgtcct ctccccggca tccgggcgcc
     1021 cgatcgtcac accgtcccag gacatggtga tcggcgtcta ctacctctcc gaggacgtgg
     1081 aggacgccaa gggagccgct cggcgcttcg ccgacttcga cgaggcgatg atggcctacg
     1141 agatcggcga gttgtcgctc catgccccga tccggattcg gctcggggag atggcaggcg
     1201 atccggagtt gtacgccgac ctcaagcaga ccttgggcag cctgttgccg gaacaaccca
     1261 tgagcagtgt cggtctctcc gacacgacgc tgggccgggt gatcttcaat caggctctgc
     1321 ccgaagggtt cccgttcgtg gattccgtgg tggtcaaggc ggacgtccgc aagctgatcg
     1381 agaccatcat cgagcgttat ccccgctcag cggtggccga gaccctcgac gggatcaagg
     1441 agctcgggtt tcactacgcc accaaggccg gtctcaccat cgcgctcgat gatgtgcgcg
     1501 ctcccgcgga caaggcacag atcctcgagg agtatgagga ccgtgcggag aaggtccaga
     1561 gtcagttcca gaagggcgtg gtcaccgacg acgagcgccg ccaagagctg atcgagatct
     1621 ggaccgaggc cactgacaag gtgaccgagg cgaccaagat cagcctcaag gagcagcgtt
     1681 tcaactccct cgacatgatg gttggctccg gtgcccgggg aaacatgatg caggtgcgtc
     1741 agatcgccgg gatgcgtggc ctg
//
LOCUS       DATITW010000518         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_466201, whole genome shotgun sequence.
ACCESSION   DATITW010000518 DATITW010000000
VERSION     DATITW010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466201"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VLS86_07215"
     CDS             complement(<1..1138)
                     /locus_tag="VLS86_07215"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HSO50321.1"
                     /translation="MKPVMSAEEYQRVDRAYAGNLDEAMNRAGFGVALAVARRGIGYG
                     SRVAILTGPGNNGGDGYVAARHLRRRGVDVEIHALAPPRTPEAIRAASRAGAARVPVR
                     DLGAVIEADLVVDALFGGGVRGSLPAEIVAWMGTDAPVVAVDYPTGLDPNTGEVPERA
                     FRAVETVTFQCIKTGHVLGHGPDYCGKLTVVDIGIEGGEPAMWVAEAEDAPRPDRPRR
                     AHKWSAGSVLVVGGSTGMVGASVLAGRAALHFGAGSVVVASPRSELVTAAAPELLTLS
                     LDDAEASLDRFDVVIAGPGLASSDRRGAIHFLDKAERLVLDAGALEPEMVDAARNGGA
                     EIVVTPHAREFERIADMESGTHAARAYATKTGMIVLLKGNPTSVT"
     gene            complement(1201..2319)
                     /locus_tag="VLS86_07220"
     CDS             complement(1201..2319)
                     /locus_tag="VLS86_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015175113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSO50322.1"
                     /translation="MTRIYLSPPDVGDNDKAAIVRAFDGGWIAPLGPEVDAFEVELAE
                     ATDRAHCAALSSGTAALHLALEVLGVGSGDRVLVSSLTFAATANAIRYTGAEPVFVDS
                     DETTWNMDPDLLGEALQSHGPFAACVVVDLYGQTADYERIVPLCAEAGVPLVEDAAEA
                     LGATYHGRKAGAFGAIATLSFNGNKIFTTSGGGALVTDNEDWAKRTRWLATQAREPGP
                     HYEHREIGYNYRLSNLLAALGRSQLADLGRRVAIRRDHNRFYRETLSDLPGVAFMPEA
                     QGAESTFWLTCITIDPTVAGTDREEVRLYLESLDIEARPVWKPMHLQPVFAESQTFGG
                     SVSERLFTNGLCLPSGSTLAPEDRERVVTAVRKVFEKA"
     gene            complement(2333..>2974)
                     /locus_tag="VLS86_07225"
     CDS             complement(2333..>2974)
                     /locus_tag="VLS86_07225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSO50323.1"
                     /translation="VDPARVTVIPRGVPRQDLDNPASREELGLPQGPLVVNVGRLAPQ
                     KGQTYLIDAFARARETVPTAHLVIVGKAGAAEQEVLAAIDRDGLAESVTIAGYSTRVS
                     DYLAHAHVFAFPSVMEGLGTAVIEAMACGVPVVAFDILPVREATVGGEYGTLVPVGDV
                     AALAVALTRHLSAERAVDVRARDWVRSHHDLDRIASLVESLLRASAGAAEERG"
BASE COUNT          495 a         1047 c          963 g          469 t
ORIGIN      
        1 cggtgaccga ggttgggttt cccttcagga gcacgatcat cccggtcttc gtggcgtagg
       61 cccgcgccgc atgggtgccg ctttccatgt cggcgattcg ctcgaactcc cgggcgtgag
      121 gggtgaccac gatctcggcg ccaccgtttc gagcagcatc gaccatctcc ggctccaggg
      181 cgccggcatc gaggaccaga cgctccgctt tgtccaggaa atggattgcc cctctgcgat
      241 cagaagatgc cagacccggt ccggcgatca cgacatcgaa tcggtcgagg ctcgcctccg
      301 cgtcatcgag ggaaagcgtc agcagctccg gagccgccgc cgtcaccagc tcggatcgcg
      361 gggaggcgac gaccaccgac cctgccccga agtggagcgc agctcgcccc gccagaaccg
      421 acgctccgac catcccggtc gaccccccga ccacgaggac ggagccagcg gaccatttgt
      481 gtgctcgccg tggccggtcc ggcctggggg catcctcggc ctcggcgacc cacatcgccg
      541 gctctccacc ctcgatgccg atgtcgacga ctgtcagctt tccgcagtag tcggggccgt
      601 gtcccaggac gtggccggtc ttgatgcact ggaaggtgac ggtctccacg gccctgaagg
      661 ctcgctcggg aacctccccg gtgttggggt cgagcccggt cggatagtcc accgctacta
      721 ccggggcgtc ggtgcccatc caggccacga tctcagcggg cagagagccg cggactccgc
      781 ctccgaacaa cgcatcgacc acgagatctg cctcgatcac cgcaccgaga tctcgtactg
      841 gcaccctggc tgcaccagcg cgtgacgcgg ccctgatcgc ctcgggtgtc cgcggcgggg
      901 caagggcgtg gatctcgacg tcgacacctc gtcgtctcaa gtggcgagcc gcgacatagc
      961 cgtctccacc gttgttgccc gggccggtga gaatcgcgac cctcgacccg tagccgattc
     1021 cgcgacgtgc cacggcgagc gccaccccga atccggcgcg gttcatcgcc tcatccaggt
     1081 ttccggcata ggcccggtcg acgcgttggt actcctcggc ggacatgacc ggcttcaccc
     1141 cgctgaggct acctgccggg attcgaccat cgaccgggtg actccggccc cgaggcgggc
     1201 tcaggccttc tcgaacacct tgcggacggc tgtgaccacg cgctcgcgat cctcgggggc
     1261 cagggtcgag ccactgggca gacagagccc gttggtgaac aatcgctccg agacggatcc
     1321 tccgaaggtc tggctctcgg caaacaccgg ctggaggtgc atcggcttcc agaccggtct
     1381 tgcctcgatg tcgagcgact cgagatacag ccggacctcc tcccggtcgg tgcccgccac
     1441 ggtgggatcg atggtgatgc atgtcagcca gaacgtggac tcggcaccct gagcctccgg
     1501 catgaaagcc accccgggca ggtcggagag agtctcccga tagaaccggt tgtgatccct
     1561 gcggatggcc acccgacggc ccagatcggc gagttgggag cggcccaggg cagcaagcag
     1621 attcgagagc cggtagttgt aaccgatctc gcggtgctca tagtgaggcc ccggctccct
     1681 ggcctgggtc gccagccaac gggtgcgctt cgcccagtcc tcgttgtcgg tcaccagcgc
     1741 cccaccaccg ctcgtggtga agatcttgtt gccgttgaac gacagggtgg cgatcgctcc
     1801 gaaggctccg gccttgcgac cgtggtaggt ggcccccagc gcctcggcag catcctcgac
     1861 gaggggcaca ccggcctcag cacagagcgg gacgatgcgc tcgtagtcgg cggtctgccc
     1921 gtagaggtcg accaccacac acgcggcgaa gggcccgtgc gactgcaatg cctccccgag
     1981 caggtcgggg tccatgttcc atgtcgtctc gtcgctgtcg acgaagacgg gctcggcgcc
     2041 ggtgtatctg atcgcattgg cggtcgcggc gaaggtcagc gaggagacga gcacccggtc
     2101 gcccgacccg accccgagca cctcgagcgc cagatggaga gctgcggtcc cactggagag
     2161 ggcggcacaa tgggcacgat ccgtcgcctc ggccagctct acctcgaaag cgtcgacttc
     2221 ggggcccaac ggggcgatcc agccgccatc gaaggcacgg acgatggccg ccttgtcatt
     2281 gtcaccgaca tccggaggcg agagatagat gcgcgtcatc gttcccgcga ggctaacccc
     2341 gctcctccgc cgcgccagcg gatgctctga gcagagactc gaccagtgag gcgatgcgat
     2401 cgaggtcgtg gtggcttctc acccaatcac gggccctgac atcgacggcg cgctccgcgg
     2461 acaggtgacg ggtcagtgcc accgccagcg ccgcgacgtc cccgaccggc accaacgtgc
     2521 cgtactcgcc cccgaccgtc gcttcccgga ccgggaggat gtcgaatgcc accaccggca
     2581 ccccgcaggc catggcctcg atgaccgcgg tgcccaatcc ctccatcacc gaggggaagg
     2641 caaagacatg ggcatgagcc aggtagtcgc tcacacgcgt cgaatagccg gcgatggtga
     2701 ccgattcggc taggccgtca cgatcgatcg cagccaggac ctcctgctcg gcggcgcccg
     2761 ctttgccaac gatcaccaaa tgagccgtgg ggacggtctc ccgtgccctg gcgaaggcgt
     2821 cgatgagata cgtctggccc ttctgtgggg cgagtctccc cacgttcacc accagcgggc
     2881 cctggggcag gcccagctcc tctcgtgagg cggggttgtc cagatcctgc cgtggaacac
     2941 cgcgcgggat caccgtcacc cgcgccgggt caac
//
LOCUS       DATITW010000519         1853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_426371, whole genome shotgun sequence.
ACCESSION   DATITW010000519 DATITW010000000
VERSION     DATITW010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426371"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /gene="pknB"
                     /locus_tag="VLS86_07230"
     CDS             complement(<1..861)
                     /gene="pknB"
                     /locus_tag="VLS86_07230"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HSO50324.1"
                     /translation="MNTPSELTERYQVTSHLARGGMADVYEGQDTLLNRRVAIKVLHA
                     QYSADEAFVKRFRREAQAAANLSHPNIVGIYDWGQAQGTYFIVMELVDGRSLRDVLKS
                     EGALLARRATEIAAEVAAALSVAHQAGLVHRDVKPGNILLAKDGTVKVTDFGIARAWD
                     DSAELTKTGAVIGTATYFSPEQAQGAGADARSDVYALGVVLYEMLTGRPPFTGDSPMS
                     VAFQHVSTEAPAPSALNSDVPASLDTVVAKAMRKDPNGRYQSADEMRADLVSVLKGQS
                     VEPAAVAAVAA"
     gene            complement(899..>1853)
                     /locus_tag="VLS86_07235"
     CDS             complement(899..>1853)
                     /locus_tag="VLS86_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HSO50325.1"
                     /translation="LASASSPSFNPESLLGEDAQANWDALLADPESPLSDRATKELFA
                     PGSTFKTVVTATALDTGEADPGTEFPDPLVYELEGSTGTISNFDGGVCEDGDTVSLLV
                     AFVRSCNTIFADLSVRLGAEDIGITAGALGFNTDLTFPWSVPQATYPVEALIDDPAAL
                     AQSGIGERDVRATPLHMAMVAAAVANQGLVPSPQLVNRVFDADGNTVEEPEPSSLGRA
                     MAPATASVLAQMMERVVTEGTGRNATVPGIRVAGKTGTAEGAGDGPHAWFIGFAPVEN
                     PTIAIAVLIAGGGDFGESATGGAVAAPIAGDLISLWLQGTP"
BASE COUNT          296 a          610 c          627 g          320 t
ORIGIN      
        1 ggccgccacc gcggccactg cggccggctc cacgctctgt cccttgagca ccgacaccag
       61 gtccgcccgc atctcgtcgg cactctgata tctcccgttg gggtccttgc gcatcgcctt
      121 ggcgaccacg gtgtccagtg aggcggggac gtcggagttg agcgcagaag gggcgggagc
      181 ctcggtggag acatgctgga acgccaccga catcgggctg tccccggtga acgggggtct
      241 cccggtgagc atctcataga ggaccacacc gagcgcatag acatcgctcc gggcgtcagc
      301 gccggcgccc tgtgcctgtt ccgggctgaa ataggtggcg gtgccgatca ctgcgccggt
      361 cttggtcaat tcggcgctgt cgtcccacgc tctggcgatc ccgaagtcgg tgaccttcac
      421 cgtcccgtcc ttggcgagca ggatgttccc gggcttgacg tcgcgatgga ccagccccgc
      481 ctgatgggcg actgacaggg cagctgccac ctcggcggcg atctcggtgg ccctgcgggc
      541 gagcaacgcc ccttcggact tgagcacgtc tcgcaacgag cggccgtcga ccagttccat
      601 gacgatgaag taggtgccct gggcctgtcc ccagtcgtag atgccgacga tgttggggtg
      661 gctgagattg gctgcagcct gcgcctcacg gcggaagcgc ttcacgaacg cctcgtcagc
      721 ggagtattgg gcgtgaagga ccttgatcgc cacccgccgg ttgagcaggg tgtcctggcc
      781 ctcgtagacg tcggccatcc cgccccgtgc cagatgggac gtgacctgat agcgctcggt
      841 cagttcacta ggggtgttca tgcggagcga tggattgtag gaacgccctg gggccaactc
      901 atggggttcc ctgcaaccag agtgaaatca ggtctcccgc gatcggcgcc gcaaccgcgc
      961 caccggtggc cgactcgccg aaatcgccgc ctccggcgat gaggaccgcg atcgcgatcg
     1021 tggggttctc gaccggagcg aacccgatga accaggcgtg gggcccgtcc cccgctccct
     1081 cggcggtccc ggtcttgccg gcgacgcgga tcccgggcac ggtcgcattc ctcccggttc
     1141 cctcggtgac gacccgctcc atcatctggg ccagaaccga ggccgtggca ggggccatcg
     1201 cccgccccag tgacgacggc tcgggctcct cgacggtgtt cccatcggca tcgaagactc
     1261 gattgaccag ttgtgggctt gggaccagac cctggttggc gacggctgcg gcgaccattg
     1321 ccatatgcaa cggggtggcg cgcacatcgc gttccccgat cccgctctgg gccagggcgg
     1381 caggatcgtc gatcagggcc tccaccgggt aggtggcctg ggggaccgac cagggaaagg
     1441 tcaggtcggt gttgaagccg agggcgcccg cggtgatccc gatgtcttca gcccccagcc
     1501 gaaccgagag atcggcgaag atcgtgttac aggaccgaac gaaggcgacc agcaggctca
     1561 cggtgtcacc gtcttcgcat acccctccat cgaagttgga gatggtgcct gtggaaccct
     1621 ccagctcata taccagtggg tcggggaact cggtcccggg gtcggcctca ccggtgtcga
     1681 gggcggtggc cgtcaccacc gtcttgaaag tagatccggg ggcgaacaac tcctttgttg
     1741 cccgatcact gagtgggctc tccgggtcgg cgagcagagc atcccagttc gcctgggcgt
     1801 cctccccgag cagagactcc gggttgaacg acggtgagga ggccgatgcc agc
//
LOCUS       DATITW010000520         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_306116, whole genome shotgun sequence.
ACCESSION   DATITW010000520 DATITW010000000
VERSION     DATITW010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306116"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(842..1681)
                     /locus_tag="VLS86_07240"
     CDS             complement(842..1681)
                     /locus_tag="VLS86_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSO50326.1"
                     /translation="MGRTPPIDAKAFNAFEAEGWEGRVDAYDHFFTPITGRLFDPLLD
                     AVRVVPGTRLLDVATGPGHLPARAAERGAVPVGVDVADAMVTRARELYPTLEFHRGDA
                     EALPFADAEFDAVTANFLLPHLARPERAMAELVRVLAPGGRLALATWDVPSRARLFGF
                     FLEAFSEVGAAPPSDLPAGPDVFRFADDAAFTDLLATAGLRDVEVSPIDFNYEAATFD
                     EYWQALQEGTVRMSSLLHGQSKETRLRIRDALKQKLDEHRAPTDTITLPVAVKLAAGR
                     KLS"
BASE COUNT          391 a          687 c          734 g          437 t
ORIGIN      
        1 gcgcgtgcac tccgtaccag accatgaacg cacgatcttc cttgaggtgc agacgattcg
       61 ctagctcctc cacctgctgt cgcatcgtcg gctcgaactt gggggtcatc gttgaagcgt
      121 aagggatcgt gaccaagggc acccgctaag tgcgggactc cctgagttcg agacgtatgg
      181 atcgatagat cgatcaaggc atgcccatgg agcccgggag ccaagttggg agccattctc
      241 tgtggattca tccagacggt cgtggacgtc agtggactcg aaaaccgttt gtgccgggcg
      301 ctctggacgt tcctggtcgt ctgtggatac cgcttggaga tctacggatc aggaggttgg
      361 gtgttcgagt cgccccgggc gcgctacatg aaaccgcctg atgaggtggc tgtatcggca
      421 ttcgaaatcc gggagccaat cgacctttct gacgtccgag tgcccaggat cgtcgagacc
      481 cacgagaccc gtccgcgtct cacaccatcc tcccccggct cgcccttcaa ccacgacacc
      541 gtgctacggg cccgccgact tctctgacac gcagcccgct ctagaccctt gtcagccccc
      601 tccgctgaga gccacgttgg gagccattcg gggtggaccg cggcggtcgt ggacggacac
      661 tggtggaaat ggaagcccgc cgctgaacca tctgtggacg gatatggacg cccacggaca
      721 aggcttggag atctacggat caggccggcg acttcgcctt caaaggtcgg cgacctcgac
      781 gcattcaacc gtccggaccg cctgcgcatg cctcgctcgc tgcccgacga tgtcgatttc
      841 gtcagctcaa ctttctcccg gcggcgagct tgaccgccac agggagtgtg atcgtgtcgg
      901 ttggggcgcg gtgctcgtcg agcttctgtt tcagggcatc acggattcgc aggcgggtct
      961 ccttagactg gccgtgtagc agcgatgaca tgcggaccgt cccctcctgc aaagcctgcc
     1021 agtactcgtc gaacgtggcg gcctcgtagt tgaagtcgat cggggacact tccacatctc
     1081 gcagccctgc tgtagcgagc agatcggtga aggcggcatc gtcggcgaag cgaaagacgt
     1141 cgggtcctgc cgggaggtct gacggtggtg cggcaccgac ctcgctgaaa gcctcgagga
     1201 agaatccgaa cagcctcgcc ctggaaggca catcccatgt cgccaaggcc agccggccgc
     1261 caggagcgag cactctgacc agttccgcca tggcccgctc gggccgcgcc agatggggga
     1321 gcaggaagtt ggcggtgacc gcgtcaaact cagcatctgc gaacggcagt gcttccgcat
     1381 ccccgcggtg aaactccagc gttggataca gttcccgcgc acgtgtgacc atggcgtcgg
     1441 cgacgtcaac accgaccggg accgctcccc tctcggcggc ccgtgcgggc agatgccctg
     1501 gaccggtggc gacgtcgagc aggcgggtgc ccggcaccac tcgcaccgca tccagcagcg
     1561 ggtcgaagag acgcccggtg atgggtgtga agaagtggtc gtaggcgtcg acccgcccct
     1621 cccatccttc ggcttcgaag gcgttgaacg ctttggcgtc gatcggtggt gtccgtccca
     1681 tgtggtcctc ctatcggtgt tcggggttga ggaggcgaat caggctccgg cgtcctaggc
     1741 ggcgcgttgg ttgccgtggg cagagtatca gcggcgttat tgaggacgcg ggcgatgtgg
     1801 attccaggtc atgaaggtgg tgttgctggc cgttcgtaac tcgttgctca tagatcagtg
     1861 cgacgccatg cactggaccg gccactccag ggatgactac tcctcccgct cattgtggac
     1921 gagatcgagc ctggccgtcg agcctgtcga tcagcgcgtt gacgtgggag ccaagttggg
     1981 agccattccc tgcggactgc gccggacggg cgtggacggc tgtggactcg gaagtcggtc
     2041 cttcaagact ctccggacag tcctggacgt aggtggaagc ccttggagat ctacggatca
     2101 ggaggttggg tccgtcgaag tgggcagtgg caccctcgca ttgctcgtct cgggcggact
     2161 gtgcgagttc gtccttctgc ccatttggct gatcgaaaag agtttccaac caccggtcgt
     2221 gacggcggcc tgaatctggt ctcgcgagt
//
LOCUS       DATITW010000521         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_306147, whole genome shotgun sequence.
ACCESSION   DATITW010000521 DATITW010000000
VERSION     DATITW010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306147"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2313
                     /locus_tag="VLS86_07245"
     CDS             <1..2313
                     /locus_tag="VLS86_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50327.1"
                     /translation="DLQWFDSASWAVALAAAKKVSPLLLVLLTRPTAERPEQLDELAD
                     LGTSARLVLKPISEQDALALATERLGVRQIGADVAKVIVEAAEGIPFFIEELAFSLRD
                     SGALDIEDGTVKLARPADELGVPHTLSSVVLSRIDRLAPELQLTLKVASVIGRTFDTQ
                     ALAAVHPAHPGTDELANQLTDLKALDLIVESSPGSFDFKHALTRDTAYDLLLFDQRRR
                     IHRSVATFLESLPDEPREPLYALLAYHWDRGEDHTKALGYFEKTGATSLRKGANREAI
                     DAHSRSLELLHRHPEEFSDVSRLRQSQWHVEIAQAHEALGNFEEAEKSLYRALELIGL
                     HVSDQPLGRIGRLVAEMFKQVVHIVLPGVIRVPHDDEEKARLAQAARIAALIGEIYYF
                     TGNLTGFPVLNLVAINLGEKSGEPLVAGLAYSSMGYLVGTLRLRRLADRYFRRAGAAE
                     DLESHPELVHTPYVLELQEMGPGHLIAVALSESVLALTFDEWSRAREIVTEALERCDR
                     LGDKYSAGIALAVRGFVSYSSGDIEDALNDYNQLLASARQRSNREHEGWATSFVIPVL
                     LALDRLDDAQVMTGSAMSILDEVDPLTVPIIHGTRSQVQLRAGQAAEAEASAELALKA
                     IDRTPIFIYLAAFAGLLDTLLELSAAESDRSSGHARDLAKLTNQGLRKLRTFARILPF
                     ARPKYWLFRGRMEHLTGRTRRAQRSLGKGLVLAEKSGFTWDEGLLHFELARTLPTASA
                     AREAHLAEARRLFEKVRSIHDLGRVATLSV"
     gene            complement(2310..>2755)
                     /locus_tag="VLS86_07250"
     CDS             complement(2310..>2755)
                     /locus_tag="VLS86_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSO50328.1"
                     /translation="RTCRLPARQREAIMAVTPEETIKAAALAYIAGDEDGLYQLLDDG
                     VRVLGSEQRDNWDGRDHVLQRMNSELTRRRTLTNSVSGSLIEAVDALESVDQQAGLAW
                     WSGTGDLDVDGAYHREASWTVVLRRWGEEDDSEWRIVHSHFSIHR"
BASE COUNT          519 a          893 c          827 g          516 t
ORIGIN      
        1 gatctccagt ggttcgactc ggcttcgtgg gccgtggcat tggcggcggc gaagaaggtg
       61 agccccctgt tgttggtact tctgaccaga ccaacggccg agcggccgga gcagttggac
      121 gagctcgctg acttggggac ctcggctcgc ctggttctga agccgatcag cgagcaggat
      181 gccctcgccc tggcaacgga gcggctcggg gttcgccaga ttggcgccga cgtcgccaag
      241 gtgatagtcg aggccgcgga aggaatcccg ttcttcatcg aggagctggc cttctcgctt
      301 cgtgactctg gtgctctcga catcgaggac gggacggtca aactcgcccg acccgccgat
      361 gagttgggtg tcccccacac tttgagcagc gtcgtcctga gccgcatcga ccgactcgcc
      421 cccgagctcc agctcacgct caaggttgcc agcgttatcg gcagaacctt cgacacgcag
      481 gcactggcag cggtacatcc cgctcacccc gggacggacg agctggccaa ccagctcaca
      541 gatctgaagg cgctcgatct gatcgtcgag tcctcgccgg gatcgttcga cttcaagcac
      601 gccttaaccc gtgacacggc atatgaccta ttgctgttcg atcaacgaag gcgaatccac
      661 cgcagcgtcg ccaccttcct cgagtctctg cccgatgaac ctcgagaacc cctatacgca
      721 ctactggcct accactggga tcgtggcgaa gaccacacga aggccctggg ctacttcgag
      781 aagacggggg ccacctccct gcgcaagggt gccaatcggg aggccatcga tgctcactcc
      841 aggtcactcg agctgctcca tcgtcatccc gaggagttct ccgacgtgag ccggctccga
      901 cagagccagt ggcacgtcga gatcgcacag gcccatgaag cgctgggaaa tttcgaggaa
      961 gcagagaaga gcctgtaccg cgccttggag ctgatcggcc tccacgtatc ggatcagcca
     1021 ctgggcagga ttggacgtct ggtcgccgag atgttcaagc aggtggttca cattgttctg
     1081 ccgggagtga tcagggtccc ccacgacgac gaggaaaagg cgcgactggc tcaggcagca
     1141 cgcatcgcag cactgatcgg cgagatctac tacttcaccg ggaacctgac tgggttcccg
     1201 gtcttgaacc tggtggccat aaatctcggc gagaagtccg gcgagcctct ggtcgcaggc
     1261 ttggcgtaca gcagcatggg gtacctggtc ggaacccttc gactaagacg tctcgccgac
     1321 cgatacttcc gtcgggccgg agcagcagaa gatctggaaa gccatccgga actggtgcac
     1381 accccctacg tcctcgagct gcaagagatg ggccctggcc atctcatcgc agtggctctg
     1441 tcggagagcg tgctcgcact gactttcgac gagtggagcc gggcccggga gatcgtgacc
     1501 gaggctctcg agcgatgtga ccgcctcggt gacaagtatt cggccggcat cgcccttgcc
     1561 gttcgtggct tcgtcagcta ctcctcggga gacatcgagg atgccttgaa cgactacaac
     1621 cagctcctcg cctccgctcg tcaacgctcc aaccgggagc atgagggatg ggcaacttcg
     1681 tttgtgattc cggtgttgct ggccctcgat cgtctcgacg atgcccaggt gatgaccggc
     1741 tcagccatga gcatcctcga cgaggtcgac cccctcaccg tgcccataat ccacgggaca
     1801 cgatcccagg tacagctgcg ggccgggcag gccgccgagg cggaggcgag tgccgaactg
     1861 gctttgaaag ccatagaccg gacgccgatc ttcatctact tggcggcgtt cgccggactg
     1921 ctcgacacgc tgcttgagct ctcggcagcg gagagtgacc gctcctcggg acacgccagg
     1981 gatctggcca agctcaccaa ccagggtctc cggaagttga gaaccttcgc tcggatcttg
     2041 cccttcgccc gcccgaagta ctggctgttc aggggacgga tggagcacct gacagggcgg
     2101 acccgaaggg cacaacgctc tctgggcaaa ggactcgtcc ttgcagaaaa gagtggtttc
     2161 acctgggacg agggtctgct ccatttcgag ttggcccgca cgctcccgac cgccagcgcg
     2221 gctcgagaag ctcatctggc cgaggcgcgc cgattgttcg agaaggtgag gagcatccac
     2281 gacctcggcc gggtcgcgac cctgagcgtc tagcggtgta tcgagaagtg agagtggacg
     2341 atcctccact cggagtcgtc ttcctcaccc catctccgca ataccacggt ccacgaggct
     2401 tcccgatgat atgccccgtc gacatccaga tctcccgtgc ccgaccacca ggccaagcct
     2461 gcctgctggt cgaccgactc gagggcatcc accgcttcga tcagtgaccc actgacgctg
     2521 ttcgtgaggg ttctgcgccg tgtcagttct gagttcatgc gttggagcac atgatcccga
     2581 ccatcccaat tgtcccgttg ctcactgccg agcacccgaa ccccgtcgtc caacagctga
     2641 tacagcccgt cctcgtcacc agcgatgtat gccagtgctg cagccttgat cgtctcctcg
     2701 ggcgttactg ccatgattgc ctcccgttgc cttgcgggaa gcctacaagt ccggc
//
LOCUS       DATITW010000522         1543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_265968, whole genome shotgun sequence.
ACCESSION   DATITW010000522 DATITW010000000
VERSION     DATITW010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265968"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VLS86_07255"
     CDS             <1..653
                     /locus_tag="VLS86_07255"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HSO50329.1"
                     /translation="GRDTDSESDRVVVDETEVRDYLGKPKVWKDEASDRTDVPGVATG
                     LAVTGAGGDVLFVEASAMDGEPGLTLTGQLGDVMKESARIALSYVEAHAEQLGIDPET
                     LTRRYHVHVPAGAIPKDGPSAGVTMLTALVSLLTGRKVRDTVGMTGEITLQGKVLPIG
                     GVKQKVLAAHRAGLKEVIVPRRNEADLDEVPEKVREEMTFHIADTAADVLWAALAE"
     gene            complement(1096..1404)
                     /locus_tag="VLS86_07260"
     CDS             complement(1096..1404)
                     /locus_tag="VLS86_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSO50330.1"
                     /translation="MVHVETTRIGQQTVVVVRGEVDLASADQLQSAIARYSGQVLTVD
                     LRGVDFIDSTGLAVLIKEHDRLGETGGELKLVVKDKGPVSRILELTGLIESFPISNTL
                     "
BASE COUNT          315 a          488 c          484 g          256 t
ORIGIN      
        1 gaggcaggga taccgattct gagtcagatc gggtcgtggt cgacgagacc gaagtcaggg
       61 actatctggg caagcccaag gtctggaagg acgaggcctc cgaccgcacc gacgtgcccg
      121 gggtcgccac cggcctggcg gtcaccggcg ccggtgggga tgtgctgttc gtcgaagcga
      181 gcgccatgga cggtgagccg ggtctcaccc tcaccggcca gctcggcgac gtgatgaagg
      241 agtcggcgag gatcgccctg tcctatgtcg aagcccacgc cgaacagctc ggcatcgatc
      301 ccgagacgct gaccaggagg tatcacgttc acgtgccagc aggggcgatc ccgaaagatg
      361 ggccgtcggc cggagtgacg atgctcacgg cgctggtgtc gttgctcacc gggagaaagg
      421 tgagagacac ggtcggaatg accggggaga tcacccttca gggcaaggtg ttgcccatcg
      481 gcggggtgaa gcagaaggtg ctcgccgctc accgagccgg gttgaaagag gtcatcgtcc
      541 cccgtcggaa cgaggcagac ctcgacgagg tgccggaaaa ggtccgtgag gagatgacgt
      601 tccacatcgc cgacaccgcc gccgacgtgc tgtgggcggc actggccgag tgacccaacc
      661 gcgagaagac gctggtgcta gtcgtcgtcc agctcgtcgt cgtcgagggc gctgggatcc
      721 gagacgtggc gtccttccac ccgctccacc tgacgggcaa agcgggttcg ttccacctcg
      781 ccggccagat ctgaaaggaa ttggacgctg acatgcccac agagctcgca caccacgcgt
      841 tctacacctg agctctgaaa tgaaaccgtt ttccgatgct ggcaattggt ctgtcgtcga
      901 tggcgacgta agacgcgcat tctgaatagc cctcccattg ataaccaccc gtgggacagt
      961 ctgcccgccc ccgtagggat tttcaatagg gcaagcaacc gagggtggcc aacaccgatc
     1021 aggacgtctc gaggtaatga ctgagccgat tcgacgcgga tgtcggccgc gcccgggcca
     1081 ggcgttcgtg cccggctaca gggtgttgct tatcggaaac gactcgatga gaccggtgag
     1141 ctcgaggata cgggacaccg ggcccttgtc cttcaccacc agcttcagct ctccgccggt
     1201 ctcgccgaga cggtcgtgct ctttgatcag caccgccagt cctgtcgagt cgatgaagtc
     1261 gaccccccga agatcgaccg tgagaacctg acccgagtat cgggcgatcg ccgactgcag
     1321 ctggtcggcg gaagcgagat ccacctcacc cctgaccacg accacggtct gctgcccgat
     1381 acgagtggtc tcaacatgaa ccaatggctc tatccccacg acctgccctt tccctggacc
     1441 tccaacatcc gaacctgggg acacccacct acggaaacca gatactacag acagtgaaca
     1501 acgaagctcg tcctgccgac cgcattcccg atcgacggag cga
//
LOCUS       DATITW010000523         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_66220, whole genome shotgun sequence.
ACCESSION   DATITW010000523 DATITW010000000
VERSION     DATITW010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66220"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /gene="argS"
                     /locus_tag="VLS86_07265"
     CDS             complement(<1..935)
                     /gene="argS"
                     /locus_tag="VLS86_07265"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HSO50331.1"
                     /translation="MSLLAHLSSITGEVFAELGLPAELGETVPSQRPELAQFQVNGAM
                     AASKLAGRPPREIAGEVAAKIGAHEDIASVEVAGPGFINLDLTDEAIARFAAHDSADP
                     FLGFPVTTEPENVIVDYAGPNVAKAMHVGHLRATIIGDSLARLYAFAGHKVIRDPHFG
                     DWGFQMGLLIAAIEVEQPHLPYFDPAADTFPTESPVTLDDLQLIYPAAAERARVDDEF
                     AALAREATVRLQQGDPGYLALWRHMKGVSETSQREDFAALGVNFDLWYGESTVHDRLA
                     PLVASLRASGVAEESEGALVIRVDQPDDNRDVPPL"
     gene            complement(1049..>2159)
                     /locus_tag="VLS86_07270"
     CDS             complement(1049..>2159)
                     /locus_tag="VLS86_07270"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HSO50332.1"
                     /translation="VLARAVEVAEAAPEPDEPMSRVYAKVIVPGPAATEQEATRGGEM
                     VNLIQAVNMALHEVFETHDDVVLFGQDVARRKGGVFKATLELTERFGPDRCFNAPIAE
                     SSIVGAAIGMSAAGFKVIAEIQFADYVHPAFDQLVSEAARISFRSDGRWHVPIVVRTP
                     YGAGIHGALYHSQSIESFYAHVPGLKVVVPSTPADVKGLLFTAVEDPDPVLFLEPKKL
                     YRLGKGPYPEGEHRVPLGRAAIRHQGSDLTILTYGAMAHFAMEAVPVLEELGVSPEVI
                     DLRSLKPLDWPTIEESIKKTSRALIVHEDNEFLGYGAELAAQIADKTFEWLDAPVRRY
                     ALPDVPIMPYAASLEAELYPTPEGIVRHAQDLAKY"
BASE COUNT          372 a          689 c          742 g          356 t
ORIGIN      
        1 atcaggggtg gcacgtcccg gttgtcgtcg ggctgatcga ctctgatgac cagcgctccc
       61 tcggactcct cggcgacccc ggaggcacgc agtgaggcga ccaggggggc gagacggtcg
      121 tgaacggtcg actcgccgta ccagagatcg aagttgaccc cgagtgctgc gaagtcctca
      181 cgctgtgagg tttcggacac acccttcata tgacgccaca acgccaggta tcccggatcg
      241 ccctgttgca gccggacggt cgcctccctg gccagtgccg cgaactcgtc gtcgacccgg
      301 gctcgctccg cggccgccgg gtagatcagt tgcaggtcgt cgagggtcac cggggactcc
      361 gtcgggaagg tgtcggcggc cggatcgaag taagggagat gaggttgctc gacctcgata
      421 gcggcgatga gcaggcccat ctggaagccc cagtcgccga aatgaggatc acggatcacc
      481 ttgtggccgg cgaaggcgta gagacgggcc agggagtccc cgatgatggt ggcgcggaga
      541 tggccgacgt gcatcgcctt ggcgacattg gggccggcgt agtcgacgat cacgttctcg
      601 ggctcggtgg tcaccgggaa ccccaggaag gggtcggccg aatcgtgagc ggcaaaacgg
      661 gctatcgcct cgtcggtcag gtcgaggttg atgaaaccgg ggccggccac ctcgaccgag
      721 gcgatgtcct cgtgagcacc gatcttcgcc gcgacctcgc cggctatctc ccggggcggc
      781 cggccggcca gcttggaggc ggccatggcg ccgttcacct ggaactgagc cagctccgga
      841 cgctgggagg gaaccgtttc ccccaattcg gcgggcaggc cgagctcggc gaacacctca
      901 ccggtgatcg aggagagatg ggcgagaagc gacatttccc cgagacctta ggtctggagc
      961 gggtttcccc atcctcaccc ccgtaattgg gagcgccagg ggtcatatgc gggcctgagg
     1021 ctcccaatta cgaaaagggg ggacccgtct agtacttggc caggtcctgg gcgtggcgga
     1081 cgatgccctc cggggtcggg tacagctcgg cctccaacga ggcggcgtag ggcatgatcg
     1141 gcacgtcggg cagtgcgtag cgacgaacag gtgcgtcgag ccactcgaag gtcttgtccg
     1201 cgatctgggc cgccaactcg gcgccgtagc ccaagaactc gttgtcctcg tgcacgatca
     1261 gggcgcggga ggtcttcttg atcgactctt cgatcgtcgg ccagtcgagg ggcttgagac
     1321 tgcgcagatc gatcacttcg gggctcaccc cgagctcctc cagcacgggc accgcctcca
     1381 tggcgaaatg ggccatcgcc ccataggtga ggatggtgag atcggacccc tggtggcgga
     1441 tcgccgcccg gcccagcggc acccgatgct ccccttcggg atagggcccc ttccccaggc
     1501 gatacagctt cttcggctcg aggaacagca ccggatccgg gtcctcgact gcggtgaaca
     1561 gcaacccctt gacgtcggcg ggggtggagg gcacgaccac tttcagccct ggcacgtggg
     1621 cgtagaacga ctcgatcgac tgcgagtggt agagggctcc atggatcccg gccccgtacg
     1681 gcgttcggac cacgatgggg acatgccagc ggccgtcgga ccggaatgag atcctcgccg
     1741 cctctgacac cagctggtcg aaagcggggt ggacgtagtc ggcgaattgg atctcggcga
     1801 tcaccttgaa gccggcggcc gacatcccga tggcggcgcc gacgatcgac gactcggcga
     1861 tcggggcgtt gaagcaacga tccggcccga agcgctcggt caactccagg gtggccttga
     1921 acaccccgcc cttgcgtcga gccacgtcct gtccgaagag gacgacgtca tcgtgggtct
     1981 cgaagacctc atggagtgcc atgttgacgg cctggatcag gttgaccatc tcaccgcccc
     2041 gcgttgcctc ttgttcggtt gccgccgggc cgggcactat caccttggcg taaacgcgag
     2101 acatcggctc atcgggctca ggcgccgcct cggcgacctc cacggcgcgg gccaggacc
//
LOCUS       DATITW010000524         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_506348, whole genome shotgun sequence.
ACCESSION   DATITW010000524 DATITW010000000
VERSION     DATITW010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506348"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..>2319
                     /gene="ppdK"
                     /locus_tag="VLS86_07275"
     CDS             314..>2319
                     /gene="ppdK"
                     /locus_tag="VLS86_07275"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HSO50333.1"
                     /translation="MSHLFDFSATDGTDPDLLGGKGAGLARMTSLGLPVPPGFTITTE
                     ACRLTMREGHVPEDLWEEVDSAITRLEEKTGRRFGGGPVPILLSVRSGSKFSMPGMMD
                     TVLNLGINDDVVETLIEWSDDAHFAWDAYRRFVQMYGNVVLGVDEILFQDVLTELRTS
                     AGVEDDSELDAAHLEVATRQFQAIVEELRPGQFRTDPRQQLKGAITAVFSSWNTKRAI
                     EYRRINAIPDDLGTAANVQMMVFGDLGDDSGTGVCFTRDPGTGARLVYGDYLPRAQGE
                     DVVAGIRNPLTLDQLGELHPKCHRQLLEIMDGLETHYQDMCDIEFTIEHDVLYILQTR
                     AGKRTAAAAVKVAVAMVEEGLIDRITAIKRVAPASLEQLHRPRIADHVKQSDAAVTGV
                     AASPGAAIGRAVFSSDDAVAEAKTGEVVILIRPETTPNDIHGMAAAIGILTSQGGKTS
                     HAAVVARGMGKPAVTGAAGLVVDVEGGVARTPAGEIRAGDLVTIDGSTGTVYIGSVDV
                     VAPESPPELHQLLEWADEYRVLGVWANADTAQDAVIARKHGAEGIGLARTEHMFLGER
                     LQVVQRIILSDSEEESSVALKELERVQVGDFESLLEAMDGLPVVIRLLDPPLHEFLPS
                     RFELEHEMRLNAKAGRPIDDLQSMSEHVARWEEDNPMLGLRGVRL"
BASE COUNT          441 a          737 c          745 g          396 t
ORIGIN      
        1 cagtagaccg tcgatgcgac gaggtcgccc accggtgaca ccccgaaaac cttcagcgcg
       61 ccgaaccaga tgaacaccac cgccagggat attcgcagcg tcggtatccc ccagcggcga
      121 agaaagggga tgacaacctc atccacccgg gcgatgaacg tgtccacacc tcgatgatcc
      181 cgcatcgggc ggtatgtgag aaatcgaaga ccaagaggtg acgaaagacc cctctttgtt
      241 tctcggcgga ccctattgcc cgaccgtcga gaatcgttaa cttcgtactg tccgccacaa
      301 ggaacaggaa ctcgtgtccc atctcttcga tttctccgct accgacggca ccgaccctga
      361 cttactcgga ggaaaggggg ccgggttggc ccggatgacg tcgctgggtc tgccggtccc
      421 gcccggcttc accatcacca ccgaggcttg ccggctgacc atgagggaag gacacgttcc
      481 cgaggatctc tgggaggagg tcgactccgc gatcacgcgt ctcgaggaga agaccggtcg
      541 acgcttcgga ggaggcccgg ttccgatcct gttgtccgtc cgatcgggct ccaagttctc
      601 gatgccgggg atgatggaca ccgtcctcaa cctcgggatc aacgacgacg tggtcgagac
      661 gttgatcgag tggtccgacg acgcccactt cgcctgggat gcctatcgcc gcttcgtcca
      721 gatgtacggc aacgtcgtcc tcggggtgga cgagatcctg ttccaggatg tcctgaccga
      781 gctgagaacg tcggcggggg tggaggacga ttccgagctc gacgccgctc acctcgaagt
      841 tgcgacccgg cagttccagg cgatcgtcga ggagcttcga cccggccagt tccgcaccga
      901 ccctcgccag cagctgaaag gggcgatcac cgcggtgttc agctcctgga acaccaagcg
      961 agcgatcgag tatcggagaa tcaacgctat tcccgacgac ctggggactg ccgccaatgt
     1021 gcagatgatg gtctttggcg acctcggtga cgactcggga accggcgtct gtttcaccag
     1081 ggatccggga actggtgccc gtctcgtcta tggcgactat ctgccgcgag cccagggtga
     1141 ggatgtcgtg gctgggatcc gcaacccgct gactctcgat cagctgggcg agctccatcc
     1201 caagtgccac cgccaactcc tcgagataat ggacggcctg gaaacccact atcaggacat
     1261 gtgcgacatc gaattcacca tcgaacacga cgtcctttac atccttcaga ccagagccgg
     1321 caagcgcacc gcggccgcgg cggtcaaggt ggcggtggcg atggtggaag agggtctcat
     1381 cgatcgaatc accgcgatca agcgagtcgc ccccgcctcg ctggagcaac tgcaccgccc
     1441 ccggatcgcc gatcatgtca agcaatccga cgccgcggtg accggggtcg cggcctcacc
     1501 gggggcggcg atcgggcgag ccgtgttctc ctccgacgat gcggtagcgg aggcaaagac
     1561 tggcgaggtc gtcatcctga tccgccccga gaccaccccc aacgacattc acggcatggc
     1621 tgctgcgatt gggatcctga ccagtcaggg agggaagacc tcgcacgccg ccgtggtggc
     1681 ccggggaatg ggcaagccgg cagtcaccgg agcggccggt ctcgtagttg acgtcgaggg
     1741 tggtgtcgcc cgcactcctg cgggcgagat ccgggccggt gacctggtga cgatcgatgg
     1801 ctccaccggg accgtctaca tcggcagtgt cgacgtggtc gcccccgagt cgccgcccga
     1861 actgcatcag cttctggaat gggccgatga ataccgggtg ctgggagtgt gggcgaacgc
     1921 agacaccgcc caggacgcgg tgatcgcccg taagcacggt gcggaaggga ttggcctggc
     1981 ccgaaccgag cacatgttcc tcggcgagag gcttcaggtg gtgcagcgaa tcatcctctc
     2041 cgactccgag gaggaaagct ctgtcgcgct gaaggagctg gagcgggtgc aggtcggtga
     2101 tttcgagagc ctgctcgagg ccatggacgg ccttcccgtc gtcatccggc ttctcgaccc
     2161 gccgctccac gagttcctcc cgtcacgctt cgagttggag cacgagatgc gtctcaatgc
     2221 caaggcgggc cgccccatcg acgacctgca gtcgatgtcc gagcatgtgg cgcgatggga
     2281 agaggacaac ccgatgctcg ggctgcgcgg agtccggct
//
LOCUS       DATITW010000525         1790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_26360, whole genome shotgun sequence.
ACCESSION   DATITW010000525 DATITW010000000
VERSION     DATITW010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26360"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VLS86_07280"
     CDS             <1..396
                     /locus_tag="VLS86_07280"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HSO50334.1"
                     /translation="CGDRASITQAQFVEVSGTQISVLVADSPEERQQGLKEAEVIPGG
                     ADGMLFVHDTPSRVNYVMLDVPIALDIWFFAPDGVLIGSTEMSPCPAEPCPLYSSPGA
                     VSWVLETVAGTFRFEEGAVLTGAPNGETG"
     gene            509..796
                     /gene="rpsF"
                     /locus_tag="VLS86_07285"
     CDS             509..796
                     /gene="rpsF"
                     /locus_tag="VLS86_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HSO50335.1"
                     /translation="MRDYEVMTIHRPDLAEDDIAAKVAELESYLSEQGASVKGKDLWG
                     KRRFSYEIEHLSEGYYSVVNFEAEAAAVDSLDRMLSLADEVVRHKIVRLGG"
     gene            857..1306
                     /gene="ssb"
                     /locus_tag="VLS86_07290"
     CDS             857..1306
                     /gene="ssb"
                     /locus_tag="VLS86_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSO50336.1"
                     /translation="MSNNVTLIGNLVDDPELRFTPSGVAMAKIRMAVNRRWRGNDGEW
                     QEDTSFFTGTVWREQAESAAESLQKGARVIVTGRLEQRTWETDQGDKRSVVEVQIDEI
                     GPSLRWATATVNKTARSGGGDFAATQGSDVPPAPVARDSYGPDEAPF"
     gene            1375..1599
                     /gene="rpsR"
                     /locus_tag="VLS86_07295"
     CDS             1375..1599
                     /gene="rpsR"
                     /locus_tag="VLS86_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HSO50337.1"
                     /translation="MEVRRGRPKPCAFCKARVEFIDWKEVSVLRKYISDRGKIRGRRV
                     NGNCPRHQREVAVAIKSAREMALLPYLSTR"
     gene            1596..>1790
                     /gene="rplI"
                     /locus_tag="VLS86_07300"
     CDS             1596..>1790
                     /gene="rplI"
                     /locus_tag="VLS86_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015155973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HSO50338.1"
                     /translation="MKLILISDVNELGKRGDVVDVAEGYARNFLLPKKKAVKASEGSM
                     AQAEATREARIESEARAREQA"
BASE COUNT          353 a          550 c          593 g          294 t
ORIGIN      
        1 tgcggagacc gagcctcgat cactcaggcc cagtttgtcg aggtttccgg gacccagatc
       61 agcgttctcg tcgccgacag ccccgaggag cgccagcagg gcctcaaaga ggccgaggtg
      121 atcccggggg gcgccgacgg aatgctcttc gtccacgaca cgccgagccg ggtcaactac
      181 gtgatgttgg acgtgccgat cgccctcgac atctggttct tcgctccaga cggggtgctg
      241 atcggaagca ccgagatgtc accctgccct gccgagcctt gtcccctcta ctcctcccca
      301 ggggcggtat cgtgggtgtt ggagaccgtg gccgggacct ttcggttcga agagggcgca
      361 gtgctcaccg gcgccccgaa tggggaaacc ggctgaatcc ggatacactt cgagcgaccg
      421 caccgacgtc tcgcgccgtt cggatgcgtg caacccctgc tccatcaacg ggatggggcc
      481 ttccaatcgg atgtccacag gaggtcgaat gcgcgactat gaagtaatga cgattcatcg
      541 tcccgatctt gcagaggacg acatcgcggc caaggtcgca gagctcgagt cgtatctctc
      601 cgagcaaggg gcgagcgtca agggcaagga tctctggggc aagcgtcgct tctcctacga
      661 gatcgagcat ctctccgagg gttactactc ggtggtgaac ttcgaagctg aggcggcagc
      721 cgtcgattca ctggatcgga tgctgtccct cgccgacgaa gtggttcgac acaagatcgt
      781 gcggctcggc ggctgactgg aatacgtcac accggcgcgg catgatcgtc ggtggatgtg
      841 agaagaggag atctcaatgt cgaacaacgt cacactcatc ggcaacctcg tcgacgaccc
      901 cgagcttcgc ttcaccccgt caggggtggc gatggccaag atccgcatgg cggtcaaccg
      961 ccggtggcgg ggcaacgacg gggagtggca ggaggacacc agcttcttca ccggcacggt
     1021 gtggagagag caggccgaat ccgccgccga gtcgctgcag aagggggcgc gggtgatcgt
     1081 caccggcagg ctcgagcagc ggacctggga aaccgaccag ggtgacaagc gatcggtcgt
     1141 cgaggtccag atcgacgaga tcggcccctc gctgcgctgg gccacggcca cggtcaacaa
     1201 gaccgcacgg tccggtggtg gcgatttcgc cgccacccag ggcagcgacg tcccaccggc
     1261 tccggtcgcc cgtgacagct acgggcccga cgaggccccg ttctgatttc aggagtttca
     1321 aactagatgg cacgcaaatc caccaataac ccacgaccga agagacgtcg tgggatggaa
     1381 gtgcgccggg gtcgtccgaa gccctgtgcg ttctgcaagg caagggtcga gttcatcgac
     1441 tggaaggaag tctccgtcct tcgcaagtac atctccgacc ggggcaagat ccgggggcgt
     1501 cgggtcaacg gcaactgccc gcggcatcag cgtgaggtgg cggttgccat caagagcgcc
     1561 cgcgagatgg cgcttctccc ctatctgagc actcgatgaa gctcatcctg atctccgacg
     1621 tcaacgagct cggcaaacga ggcgacgtgg tcgacgtggc tgagggctac gcccgcaact
     1681 tcctactgcc caagaagaag gcagtgaagg ccagcgaggg ctcgatggct caagccgagg
     1741 ccacccgtga ggctcggatc gagagtgagg cccgggcacg ggagcaggcg
//
LOCUS       DATITW010000526         1801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_426683, whole genome shotgun sequence.
ACCESSION   DATITW010000526 DATITW010000000
VERSION     DATITW010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426683"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VLS86_07305"
     CDS             <1..240
                     /locus_tag="VLS86_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50339.1"
                     /translation="PVDKDIYDLPPAELAGLPSVPGSLPEALEALANDHEFLLQGGVF
                     SKELIDAWIDYKIEHEVDPVRLRPHPHEFELYYDI"
     gene            complement(358..1572)
                     /locus_tag="VLS86_07310"
     CDS             complement(358..1572)
                     /locus_tag="VLS86_07310"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSO50340.1"
                     /translation="MPVEFRAIRADELDDLRSAVGFAFGFDPHPDDHGFSAVVPLDRT
                     RCGFDNGRIISTSAAFSLQMTVPGGVVPCGGTTIVSVSPTHRRRGVMREMMRSHLDDV
                     RDHEEPLAALWATDSAIYGRFGFGCAAVCHEIEVKRAHVDLSRLAPEPAPVRPLDVDE
                     AAAVLPAFFDSVRGAIPGFYARDPKWWEHRHFSDLEHRREGMTKHRYVVVDSGDGIAG
                     YAQYRSKFGWDEGHPAGHLEVLELIGTTPESWAGLWAYVLNHDLLERITADIRPPWDP
                     LFDLLAGTRRVGAKRSDSMWVRIMDVPAALSARAYSGPVDLVLAVSDPIGDTTGAYQL
                     RVDGETVECAVTGKEPDVTLDLEDLSACYMGRARFRELARAGRVSGEGAALAELDRAF
                     TWDPQPWCPEVF"
     gene            complement(1572..>1801)
                     /locus_tag="VLS86_07315"
     CDS             complement(1572..>1801)
                     /locus_tag="VLS86_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50341.1"
                     /translation="VAALTAPAGLEANQEALAAQASLAARAADEALSGLRSTDPGELR
                     RAAVAAFTAAVADFDVEVANARTAAGESPDA"
BASE COUNT          307 a          610 c          607 g          277 t
ORIGIN      
        1 ccggtggaca aggacatcta cgacctgccg ccggccgagc tcgccggtct gccctcggtc
       61 ccggggagcc tccccgaggc gctggaggcg ctggccaacg accacgagtt cctgctccag
      121 ggcggtgtct tctccaagga gttgatcgac gcctggatcg actacaagat cgagcacgag
      181 gtcgacccgg tccgtctccg gcctcatcca cacgagttcg agctgtacta cgacatctga
      241 gggtgcggcc ccctccgggg gccgcaccac cggtttacgg actgcggact gcgtgatacg
      301 tgcgacgtgg gacgtgaagg gcgagggccc gtcggcagac gggccctcac gcccgcatca
      361 gaagacctcc gggcaccagg gctggggatc ccaggtgaaa gcgcggtcca attcggccaa
      421 ggcagcaccc tcgccggaga cacgcccggc tcgggccaac tcccggaacc tggcccgccc
      481 catgtaacag gcgctgaggt cctccagatc gagggtgacg tctggctcct taccggtgac
      541 agcacattcg actgtctccc cgtcgaccct gagctggtag gccccggtgg tgtcaccgat
      601 tggatcggag acagcgagaa ccagatccac cggccctgaa tatgccctgg cgctcaacgc
      661 ggcggggacg tccatgatcc gcacccacat cgagtccgac cgcttggctc cgaccctcct
      721 ggtgccggcg aggagatcga acagcgggtc ccacggaggc cggatatcgg cggtgatccg
      781 ctccagcagg tcgtggttca agacgtaggc ccagaggcct gcccaggact ctggcgtggt
      841 gccgatcagt tccaggactt cgagatgccc ggctgggtgt ccttcgtccc agccgaactt
      901 ggagcggtac tgggcgtatc cagcgatccc gtccccggaa tcgaccacga cgtatcgatg
      961 cttggtcatc ccctcccggc ggtgctcgag atccgagaag tgtcgatgct cccaccattt
     1021 gggatcgcgt gcatagaaac ccgggatggc gcccctgacc gaatcgaaga aggccgggag
     1081 caccgcggcg gcctcgtcga cgtcgagggg gcggactggt gccggctcag gcgccagtcg
     1141 actcaggtcc acgtgtgctc gcttgacctc gatctcgtgg caaacggcgg cgcaaccgaa
     1201 tccgaaccgg ccatagatgg cgctgtccgt cgcccagagt gcggccagag gctcttcgtg
     1261 gtcgcggacg tcgtcgagat gcgacctcat catctcccgc atcacgcctc gtctgcgatg
     1321 agtgggcgac accgagacga tcgtggtgcc accacagggc accacgccac cagggacggt
     1381 catctggagg gagaacgcgg ccgacgtgct gatgatccga ccgttgtcga aaccgcagcg
     1441 ggtccggtcg agggggacca ctgcgctgaa accgtgatcg tcggggtgcg gatcgaaccc
     1501 gaaggcaaac cccaccgcgc ttctgaggtc gtcgagctcg tcggcccgga tggcccggaa
     1561 ctcgaccggc atcaggcgtc gggcgattcg cccgcggcag tccgtgcgtt ggcgacttct
     1621 acgtcgaaat cggcgaccgc ggcggtgaag gcggccaccg ccgcccggcg cagctcaccc
     1681 gggtcggtgg agcgcaaacc ggaaagggcc tcgtccgcgg cgcgggcggc gaggctggcc
     1741 tgcgcggcca gggcctcctg gttcgcctcg agaccggccg gagcggtcaa cgcggctacc
     1801 t
//
LOCUS       DATITW010000527         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_506681, whole genome shotgun sequence.
ACCESSION   DATITW010000527 DATITW010000000
VERSION     DATITW010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506681"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VLS86_07320"
     CDS             <1..542
                     /locus_tag="VLS86_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50342.1"
                     /translation="ALGGAFGEDTWWVRLGLLLAALGLITARNRQLSGLFQVAAGLAL
                     AIQAMRVGGWAPALSATMALGGVTGEMLLGHWYLVDPRLPRWALKSLAVAGIIGLMLD
                     SVVLAVAGLPSGGATIAFWVLMATSVILMAAVFAALRYPAYAGVMAATGLSYLAVLTS
                     LAAVFLGRVLVAGMGPFGS"
     gene            549..>1649
                     /locus_tag="VLS86_07325"
     CDS             549..>1649
                     /locus_tag="VLS86_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HSO50343.1"
                     /translation="MNVEIKIAAQDVDLTGATVMAGVLTDREPVPGSETALAGVDMNL
                     LDLAKFEGKQGQVLAVPHENAAALLLVGIGDEASYDSIRTAVGNGIRAVKTVRAVTSL
                     NQLPIEAATRAVVEGALLGGYEYREYKTSGEASVIESFELVLGDESEIAEAVIAAEVT
                     NLARDWVNTPALDMAPETLAARIAAAAGDAGVSSEVWDLDRLQEDGLGALLGVAAGSD
                     RAPALVVLEYRPKGEEAHLGLVGKGITFDTGGLSIKTAPFMEEMKDDMSGAAVVAAAT
                     IGIARIGLPIRVTAVIPLTDNALGGNATRPGDVLRPILGPTIEVLNTDAEGRLILADG
                     LGVMRRYEPDLVIDVATLTGAARVALGDRIAAV"
BASE COUNT          266 a          485 c          592 g          306 t
ORIGIN      
        1 tggcgctggg cggcgcgttc ggcgaggaca cctggtgggt caggttgggg ctgttgctcg
       61 ccgctctcgg gctgatcacg gcgcgcaacc gtcaactgtc cggtttgttc caggtggccg
      121 ccggactcgc cctggcgatc caggccatgc gggtaggagg atgggctccg gctctctccg
      181 caaccatggc tttaggtggg gtcaccggtg agatgctgct tggccattgg tatctagtcg
      241 atcccagact tccccggtgg gctctgaagt cgctcgctgt cgcagggatc atcgggctga
      301 tgctcgacag tgtggtgttg gccgtggccg gtctgccgtc gggaggggcc acgattgcct
      361 tctgggtgtt gatggcgacc tccgtgatac tcatggcggc ggtcttcgcc gcactccggt
      421 acccggccta tgccggcgtc atggcagcca ccggtttgtc atatctggct gttctcacca
      481 gtctcgcggc cgtcttcttg ggacgagttc tggtggcagg gatgggtccg ttcggtagct
      541 gaacaggagt gaatgtggag atcaagatcg cggcgcaaga tgtggatttg accggggcga
      601 cagtgatggc cggcgtgctg acagatcgcg agccggttcc gggcagcgag accgcgctgg
      661 cgggtgtcga tatgaatctt ctggatctgg cgaagttcga gggcaagcag ggccaggtgc
      721 tggcagtgcc acacgagaac gccgccgcgt tactgctggt cgggatcgga gacgaggcga
      781 gctacgactc gattcgaact gccgtgggca acggaatccg cgctgtgaag accgttcggg
      841 ctgtcacgag tctgaaccag ttgccgatcg aggctgcgac tcgggccgtc gtggaggggg
      901 cgctgctcgg aggctatgag taccgcgaat acaaaaccag tggggaagca tcggtgatcg
      961 agagctttga actggtgtta ggtgacgaat ccgagatcgc agaggccgtg atcgcggccg
     1021 aggtgaccaa cttggcgcgg gactgggtca acacaccggc gctggacatg gcgccggaga
     1081 cactcgccgc gcggatcgcg gcggctgccg gagatgccgg agtgagctcc gaggtttggg
     1141 accttgaccg actccaggag gacggcctcg gagcccttct cggagtggcc gccgggtcgg
     1201 atcgcgctcc cgccctggtg gtgctcgaat accggccgaa gggagaggag gcccatctcg
     1261 ggctggtagg gaagggaatc actttcgaca ccggcggcct ctccatcaag accgcgccat
     1321 tcatggagga gatgaaggac gacatgtccg gagccgcggt ggtggcggcg gcgaccatcg
     1381 gcattgcccg aatcgggctg ccgattcggg tgacagccgt gatcccattg accgacaatg
     1441 ccctcggcgg caacgccacc cggcccggag acgtgctgcg ccccatcctc gggcccacca
     1501 tcgaggtcct caacaccgac gccgagggtc gtctcatcct ggccgacggt ctgggtgtga
     1561 tgcgccgcta tgagcccgat ctggtcatcg acgtcgcaac cctcaccggc gccgcacgcg
     1621 tcgcgcttgg cgaccggatc gccgcagtc
//
LOCUS       DATITW010000528         1794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_346450, whole genome shotgun sequence.
ACCESSION   DATITW010000528 DATITW010000000
VERSION     DATITW010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346450"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..567)
                     /locus_tag="VLS86_07330"
     CDS             complement(124..567)
                     /locus_tag="VLS86_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019885073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HSO50344.1"
                     /translation="MKAADFEGMVGTEIGVSDWLEITQDRVDAFADATDDHQWIHVDA
                     AKAAAGPFGGTIAHGFLTLSLTVTLSSQVELDVGSPKMAINYGLEKVRFPAPVPVGSR
                     VRARVGLTSVSDVQGGIQVNRQVTIEVDGGEKPAMVAETVSRYYY"
     gene            606..>1794
                     /locus_tag="VLS86_07335"
     CDS             606..>1794
                     /locus_tag="VLS86_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HSO50345.1"
                     /translation="MPVLATKIDPRSEEYQANRAAMEAQLDEFEKLMEQARQGGGEKY
                     IQRHRKRGKLLARERIELLLDRDAPFLELSTLAAWGTDKNLGANVVTGIGVVEGVECA
                     ILAHDPTSQAGSMNRYSVAKTLRIMDIARENRLPLVNLVESGGADLPDQAEVFIVGGK
                     WFRNLTQLSKLGIPTVAIVFGSSTAGGAYFPAMCDYSVMVKDQAKVFLGGPPLVKMAT
                     GEESTDEELGGAEMHSKVSGLSDYFARDEHEAIRMGRDIVSHFNWRKQGYGPTKEADD
                     PIHDIDHILGVIPADLRVPFDMREILARTLDGSRFEEYKTDYGTSILTGWGSIHGFPV
                     GVVANQQGVIFSEEAQKATEFIQLTNRYDTPLIFIHNTTGYMVGKDYEQRGIIKDGAK
                     MVNA"
BASE COUNT          344 a          593 c          570 g          287 t
ORIGIN      
        1 gggccaccca attacgcaat ggtcggacca ccgcggcgag ttggcgacac gccgacagca
       61 gccccctccc ccgaaatcgg gagccacagg ggtcacatgc ggacctgggc cacccaatta
      121 cggctagtag tagtagcggc tcacggtctc ggccaccatg gccggcttct ccccgccgtc
      181 gacctcgatg gtgacctgac ggttgacctg gatcccaccc tggacgtcac tcaccgaggt
      241 cagaccgacc cgggccctga cccgggagcc gaccggcacc ggggccggga accgcacctt
      301 ctccagcccg tagttgatcg ccatctttgg tgacccgacg tcgagctcga cctgtgagct
      361 gagcgtgaca gtcagggaga gggtgaggaa cccatgggcg atcgtccccc cgaacgggcc
      421 ggccgccgcc ttggccgcgt cgacgtggat ccactggtgg tcgtcggtgg catcggcgaa
      481 cgcgtcgaca cgatcctggg tgatctccaa ccaatcgctg accccgatct cggtcccgac
      541 catgccctca aagtctgctg ccttcatcgg cctcaggata cgggggtgtc actagcctcg
      601 cctccgtgcc ggttctcgcc accaagatcg acccacgctc cgaggagtac caggcgaatc
      661 gcgccgccat ggaggctcag ctcgacgagt tcgagaagct gatggagcaa gcccgccagg
      721 gtggcggtga gaagtacatc caacgtcacc ggaagcgcgg caagctcctg gcgcgggagc
      781 gcatcgagct tctcctcgac cgcgacgccc cgtttctcga gctgtccaca ctcgccgcct
      841 ggggcaccga caagaacctg ggcgccaacg tcgtgaccgg gatcggggtg gtcgaggggg
      901 tcgagtgcgc gatcctcgcc cacgacccga ccagccaggc aggctcgatg aatcgatact
      961 cggtggcgaa gacgctgagg atcatggaca tcgccaggga gaaccggctc cccctggtca
     1021 atctggtcga gtcgggggga gccgacctcc ccgaccaggc cgaagtcttc atcgttggcg
     1081 gcaagtggtt ccgcaatctc acccagctct ccaagctcgg gattccgacg gtcgccatcg
     1141 tctttggcag ctcaacggcc ggcggggcct acttcccggc gatgtgtgac tactcggtga
     1201 tggtcaagga ccaggccaag gtgtttctcg gagggccgcc cctggtgaag atggccaccg
     1261 gtgaggaatc gaccgacgag gagttgggtg gtgccgagat gcactccaag gtctcggggc
     1321 tctccgacta tttcgccagg gacgagcacg aggcgatccg gatgggccgg gacatcgtca
     1381 gccacttcaa ctggaggaag cagggctatg ggccgaccaa ggaggccgac gacccgatcc
     1441 acgacatcga tcacatcctc ggggtgatcc cggccgatct ccgggttccg ttcgacatga
     1501 gagagatcct cgcccgcacc ctcgacggtt cccggttcga ggaatacaag accgactacg
     1561 ggacgtcgat cctcaccgga tggggctcga tccacgggtt cccggtcggg gtcgtcgcca
     1621 accagcaggg agtgatcttc tccgaggagg cacagaaagc caccgagttc atccagctga
     1681 ccaatcgcta cgacaccccg ctgatcttca tccacaacac caccgggtac atggtgggca
     1741 aggactacga gcagcgtggg atcatcaagg acggggcgaa gatggtcaac gcag
//
LOCUS       DATITW010000529         1584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_306648, whole genome shotgun sequence.
ACCESSION   DATITW010000529 DATITW010000000
VERSION     DATITW010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306648"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VLS86_07340"
     CDS             complement(<1..766)
                     /locus_tag="VLS86_07340"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HSO50346.1"
                     /translation="MERGIKARAMMLVEPKHFTLTELPTQPVDRGGWLAVEATGISGA
                     DIQVWKGEARDVVYPLVPGHEVVGRIATEGPTIPFPVGTRVVVEPRIRCGECHRCKQS
                     LATCVRRRPSNSYGFIPSIEPPGLWGGLAEYLFIDPDARLHQLTDDVPAEVSTFVHPL
                     AAGHTWAVELPALQTGESVLILGPGPRGLACLIAAKAAGAGWVGVTGLSHDRDRLELA
                     KRLGADRTIDIEKEDVGGAIAEAVGRRPDIVVDVTSD"
     gene            complement(803..>1584)
                     /locus_tag="VLS86_07345"
     CDS             complement(803..>1584)
                     /locus_tag="VLS86_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSO50347.1"
                     /translation="IDLAGGRTVSVPTDLADGFKVTVDGLERARTDRTKLLVFVSPSN
                     PTGAVYTAEEARVIGEWAAANDIWVMADEIYQRLTYFTDVAPSIAAVTPRFENWVLIN
                     GVAKSYSMTGWRVGWMVGPADVIETANSHQSHATGNVANVAQHAALAALTGPQETVEE
                     MRLAFDARRKLMYSMVSALPGVACIEPEGAFYVFPDFSAHLGDRWATTDDLATAILEE
                     AGVALVAGESFGTPGFLRLSYAVGEEEIERGVERIGALLAT"
BASE COUNT          269 a          540 c          517 g          258 t
ORIGIN      
        1 cgtcggaggt gacatcgacg acgatgtcgg gtctccgtcc cacggcctcg gcgatcgccc
       61 ctccgacatc ctccttctcg atgtcgatcg tccgatcggc ccccaaccgt ttcgcgagct
      121 cgagccggtc gcggtcatgg ctgaggccgg tgaccccgac ccagccggcg ccggctgcct
      181 tggccgcgat tagacaggcc agcccgcgag ggccggggcc gaggatgagg acactctcgc
      241 cggtctgcaa tgcggggagc tcgaccgccc aggtgtgccc ggcggccagg ggatggacga
      301 aggtcgagac ttcagcaggc acgtcatcgg tcagctggtg gagccgggca tcggggtcga
      361 tgaacaggta ttcggcgaga ccgccccaga gcccgggcgg ctcgatcgag gggatgaacc
      421 cgtaggagtt cgagggtcgg cgccggacac aggtggccag gctctgcttg cagcgatgac
      481 attccccaca gcgaatgcgg ggctcgacca cgacccgggt gccgacggga aacgggatgg
      541 tcgggccttc ggtggcaatc cggcccacga cctcgtgccc ggggacgagc ggatagacca
      601 catctcgggc ctcgcctttc cagacctgga tgtcggcgcc ggagatcccg gttgcctcta
      661 ccgcaagcca accaccgcgg tcaacgggtt gtgtcggcaa ctcggtcaac gtgaagtgct
      721 tgggctcgac gagcatcatg gcccgggctt tgatccctcg ctccatcgtt ggcgagtgta
      781 aacccggttc ccgggccccc agtcaagtag cgagcaacgc gccgattcgc tcgactcccc
      841 gctcgatttc ctcctcgcca accgcgtagg agagtcgaag gaagccgggg gtgccgaacg
      901 actcgccggc gaccagcgcc accccggcct cctcgaggat cgcggtcgcc agatcatccg
      961 tggtcgccca ccggtcgccc agatgcgccg agaagtcagg aaagacgtag aacgccccct
     1021 ccggctcgat gcaggcaacg ccgggcaagg cgctcaccat cgaatacatc agctttcgcc
     1081 tggcatcgaa agcgagtcgc atctcctcga ccgtctcctg gggcccggtg agggcggcga
     1141 gcgcggcatg ctgtgccacg ttggccacgt tgccggtggc gtgggattgg tggctgttgg
     1201 cggtctcgat cacatccgcc gggcccacca tccagcccac cctccaaccg gtcatcgaat
     1261 agctctttgc caccccgttg atgagcaccc agttctcgaa ccgaggtgtc accgcggcga
     1321 tcgacggagc gacatcggtg aaataggtga gacgctgata gatctcgtcg gccatcaccc
     1381 agatgtcgtt cgccgcagcc cactccccga tgacccgggc ctcctcggcg gtgtagacgg
     1441 ctccagtcgg gttcgacggc gaaacgaaca cgaggagctt ggtgcgatcg gttcgcgccc
     1501 gttccaggcc gtccacggtg accttgaacc catccgcgag atcggttggg accgacacgg
     1561 tcctccctcc ggccaggtcg attc
//
LOCUS       DATITW010000530         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_787111, whole genome shotgun sequence.
ACCESSION   DATITW010000530 DATITW010000000
VERSION     DATITW010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787111"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /gene="lexA"
                     /locus_tag="VLS86_07350"
     CDS             complement(<1..606)
                     /gene="lexA"
                     /locus_tag="VLS86_07350"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HSO50348.1"
                     /translation="MLTPTSPKGVRVLSERQQQVLEYIRKTVAERGYPPSAREIGDAV
                     GLSSPSTVHSHLSSLVAAGAITRDPSKPRAIMIVDDARQAATPATDRTLPDRLREVPI
                     LGRIAAGTPILAAEHVEDVLTLPVDLIGSDPVFLLQVKGDSMIDAGILDGDLVAIRSQ
                     KDALDGEIVAALIEGEEATVKRLRRREGKVILESENPAYEPM"
     gene            771..1046
                     /locus_tag="VLS86_07355"
     CDS             771..1046
                     /locus_tag="VLS86_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HSO50349.1"
                     /translation="MAKHQLSVRLFLLLAAVCVVFLMIGGAADADIPTPDPIQYVVEA
                     GDTLWAIAAEVAGPEQDIRRLVADITRLSGVEGDLIVPGQVLLLPGS"
     gene            1096..1545
                     /gene="nrdR"
                     /locus_tag="VLS86_07360"
     CDS             1096..1545
                     /gene="nrdR"
                     /locus_tag="VLS86_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011273655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HSO50350.1"
                     /translation="MLCPVCGAPNTRVIDSRPAEAGQAIRRRRECEDCSHRFTTYERL
                     EPQLVVRKRNGRLEAFSTAKLAVGISAALADRPVSGSAIERLAEEVEASLRGHGSQVS
                     SEDIGREVLERLRGLDEVGYLRFASVYKEFQDAADFEKEVAALESAE"
     gene            complement(1534..2529)
                     /locus_tag="VLS86_07365"
     CDS             complement(1534..2529)
                     /locus_tag="VLS86_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50351.1"
                     /translation="MGFIDSPAATWTTTPYSAPDSGPAPETTQTSGLVPIAVGKHSRH
                     VEPSGELVKDIWDSTELHAPSLLDWAPNAFSTKTLGRRDFRWPVVLIVIVGALAVAGF
                     GFWLYQQSGSSASSAITEVQTEANALDQALHQAAPLIDGLDEERLPRANQDSTVFFAM
                     DAAARAMFAASADLPSASSDDRAAAADAAALALDASRQLMDATAYRTALEPALTLPLL
                     ETDPALTDLTVATAAFAEWRSSFEAMHEALPAAGAGQAADALDRVSGGLEATQHAYLD
                     ALRTDDSHAAVEALGALRAELQSIRQALLVDMGEISNSVSGIIEQAEAKLERLLR"
     gene            2644..>2939
                     /gene="dut"
                     /locus_tag="VLS86_07370"
     CDS             2644..>2939
                     /gene="dut"
                     /locus_tag="VLS86_07370"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HSO50352.1"
                     /translation="MQVTFVRLDPEVEIPTTAHIGDAAVDLQASVEISLKPGERASVA
                     TGLAVAIPQGYAGLVIPRSGHALRAGIGVVNGPGLIDSGYRGEVSVLLINHGSE"
BASE COUNT          503 a          903 c         1007 g          526 t
ORIGIN      
        1 catcggctcg taggccgggt tctccgactc gaggatgacc ttgccctccc ggcggcggag
       61 ccgtttcacg gtcgcttctt ctccctcgat gagggcggcg actatctcgc cgtcgagggc
      121 gtctttctgg ctgcggatcg cgaccaggtc gccgtcaagg atcccggcgt cgatcatcga
      181 gtcgcccttc acctggagga ggaacacggg gtcgctgccg atcaggtcga ccggcagggt
      241 gagcacatct tcgacgtgct cggcggcgag gatcggggtc ccggcggcga tgcgtcccag
      301 gatcggcacc tcacggagcc ggtcggggag ggtgcgatcg gttgccggcg tggcggcctg
      361 ccgtgcgtcg tccacgatca tgatcgccct cggcttggag gggtctcgag tgatggcccc
      421 ggccgcgacg agtgaggaaa gatgggagtg gacggtggaa ggcgacgaga gcccgacggc
      481 atcgccgatc tctctcgccg acggcgggta tccgcgctca gcgaccgtct ttcggatgta
      541 ctccaagacc tgctgttgtc tctcgctcag caccctgact cctttcgggc ttgtcggggt
      601 gagcataatc gaacaggagt tcgcaaccaa acacctgttc ggtaccgggc gccccccgac
      661 tctcgacttg acatcgaaca actgttcgat agagtggcga acgcacgttc gataacggac
      721 ctgcatccag cagcccgcaa gggcccgatc aggaggaaga ccaaatgcag atggcgaaac
      781 accagctgtc cgtccggctg ttcttgcttc tcgcagccgt ctgcgtggtc ttcctcatga
      841 ttggtggtgc ggccgacgcc gacatcccga ccccggatcc gatccaatac gtggtcgagg
      901 ctggtgacac gctgtgggcc attgccgccg aagtggccgg tcccgagcaa gacattcgaa
      961 gactcgtggc cgacatcacc cgtctcagtg gggtcgaggg cgatctcata gttcccggtc
     1021 aggtgcttct ccttcccggc agctgaattg ccgctgtcag cagcgcagag ggtccgttgc
     1081 gtttagcctc gtgtgatgct gtgccccgtc tgcggcgccc ccaacaccag ggtcatcgat
     1141 agccggccgg cggaagccgg gcaggcgatc cgacgacgtc gcgagtgtga ggactgctct
     1201 catcgcttca ccacctatga gcggctggag ccacagctgg tggtccgcaa gcgaaacggc
     1261 agactcgagg cattctccac cgccaagctc gccgtcggga tatcagctgc gctggcggac
     1321 agacctgtgt cgggctctgc gatcgagagg ttggcggaag aggtcgaggc gtcgcttcga
     1381 ggtcacgggt ctcaggtcag ctccgaggac atcggacgtg aggtcctgga gagactgcgg
     1441 ggtctcgacg aggtcggcta tctgcgattc gcatcggtgt acaaggagtt ccaggacgcg
     1501 gccgacttcg agaaggaagt ggctgccctc gaatcagcgg agtagccgct cgagcttggc
     1561 ctccgcctgc tcgatgatcc cggagaccga gttcgagatc tcgcccatgt cgaccaggag
     1621 agcctggcgt atcgactgca actcggcgcg aagggccccg agtgcctcca ccgctgcgtg
     1681 gctgtcgtcg gttcgcagcg catcgaggta ggcgtgctgt gtcgcctcga gaccgccgct
     1741 gacccggtcg agtgcgtcgg cggcctgccc ggcaccggca gcaggcagcg cctcatgcat
     1801 ggcttcgaag ctgctccgcc actcggcgaa cgccgcggtt gccaccgtca gatcggtaag
     1861 tgccgggtcg gtctcgagaa ggggaagggt cagagccggc tcgagggcgg tgcgataggc
     1921 ggtggcgtcc atcagctggc gtgaagcgtc gagggcgagg gcggcggcgt cagccgcggc
     1981 ggcccgatcg tcagagctgg ctgacggaag atctgcggag gccgcgaaca tcgctcttgc
     2041 ggcggcgtcc atcgcgaaga agacggtcga gtcctggttg gcccggggga gtcgctcctc
     2101 gtccaggcca tcgatgagtg gcgccgcctg gtgcaaagcc tggtcgagag cgttggcctc
     2161 ggtctggacc tcggtgatcg ccgaagacgc cgagctaccc gattgctggt acagccagaa
     2221 gccgaatccg gccaccgcga gcgcccccac gatcacaatg agaacgaccg gccagcgaaa
     2281 gtcccgccga ccgagagtct tggtgctgaa tgcgtttggc gcccaatcga ggagggatgg
     2341 ggcgtgcaac tcggtcgagt cccagatgtc cttgaccaac tccccggacg gctcgacgtg
     2401 tcgggagtgc ttcccgaccg cgatcgggac caacccgctg gtctgggtgg tttcgggcgc
     2461 cggtccggaa tcgggggccg aatagggggt cgtggtccaa gtggcggcgg gggagtcgat
     2521 gaaccccaag gattccatcc acgaaaagcg caaggagcgc cggtttgtgt tcgaggtggc
     2581 ggtcaacggg cacgctcgct tacagtcgtc tggtgaacct gcccgatgcc ccgtgacaac
     2641 gagatgcagg ttactttcgt aagactggat ccggaagtgg agattcccac aactgcacat
     2701 atcggcgatg cagcggtcga tcttcaggca agcgtggaaa tttctttgaa acccggagag
     2761 cgggcgtcgg tcgccaccgg tctcgccgtc gccattccac agggctatgc gggtctggtc
     2821 atccccagaa gcgggcatgc cttgcgcgca gggatcggag tcgtcaacgg ccccggtctc
     2881 atcgactctg gataccgcgg cgaggtgtcg gttctcctca tcaaccacgg atccgagag
//
LOCUS       DATITW010000531         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_146352, whole genome shotgun sequence.
ACCESSION   DATITW010000531 DATITW010000000
VERSION     DATITW010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146352"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VLS86_07375"
     CDS             complement(<1..269)
                     /locus_tag="VLS86_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50353.1"
                     /translation="MDHLLKKFGFLEFSASNVVFGPMQWGPVRNVARKVYDLLGLHGK
                     VSLPQSVNWSKTKAYTTIRSTGEGVSINLAGRDVDGIVDPGDYEKV"
     gene            complement(342..1094)
                     /locus_tag="VLS86_07380"
     CDS             complement(342..1094)
                     /locus_tag="VLS86_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSO50354.1"
                     /translation="MTFMPRVLLIGWDGADWRILEPLLERGVLPNLQALIDRGQKGVL
                     RSTVPTHSWAAWPSFLTGVDPADHGVYDILETVPGTHKQYPVTYKSIKERTFVEDLSA
                     AGKVGVYADVPLLFPPPDINGKIAAGGVLPKGRTYTQPADLAEQLEKAGVPWVINGMS
                     WTTYRNRPEQYLDEALEITGKRIRATEWLMDNSDWDLLASVWVSVDRTQHALSNYVAP
                     DHPDTETNRETPIGWKVRDVYAQLDDAIGSFV"
     gene            complement(1165..2130)
                     /locus_tag="VLS86_07385"
     CDS             complement(1165..2130)
                     /locus_tag="VLS86_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSO50355.1"
                     /translation="MAWDEDLSTEQQEAAGVASGHLVLLAGPGTGKTFVLVRRIQYLI
                     EVVGVSPGDIQALTFSRAAAAEMRDRLVEQLGDRGERVRVSTLHSSALRQLMLHGARR
                     LPAPVRVAGDWEERWIVIEELARLLGRRVKEISNGKDGALDLLADDWETLEADGAGWE
                     AGHPDAQFLSAWQRHRQVYGYTLRSELVYQLLIELRSDPALSPEALREIVVDEYQPRL
                     EGRVTFTTMHGAKGLTADTVIVLQAEDEVIPGDGTGAYLDEARRLLYVSLTRARKKLV
                     IGACRRRIGPQRFVGEQEIEKRQLTRFLADYGLMAQTIEQYLADD"
BASE COUNT          431 a          815 c          718 g          473 t
ORIGIN      
        1 accttctcgt agtcgccggg gtcgacgatc ccgtccacgt cgcgcccggc gaggttgatg
       61 ctcacgccct cgccggtgga acggatcgtg gtgtacgcct tcgtcttcga ccaattcacc
      121 gactgcggca gactcacctt cccgtgcaga ccgagcaggt cgtagacctt gcgagcgacg
      181 ttgcgcaccg gaccccactg catcggcccg aacacgacgt tcgaggcgct gaactccagg
      241 aagccgaact tcttcagcag gtggtccatg tggatcgtgc gcgtgaccga ctggaacccg
      301 tggtccgaga tgaacaggac gaggtcgtcg ggccgcgtgc gctagacgaa cgagccgatc
      361 gcgtcgtcca gctgcgcgta gacgtcgcgc accttccagc cgatcggggt ctcgcggttc
      421 gtctcggtgt cggggtggtc gggcgcgacg tagttcgaga gcgcgtgctg cgtccggtcg
      481 accgagaccc acaccgaggc caacaggtcc cagtccgagt tgtccatcag ccactcggtc
      541 gcgcggatcc gcttgccggt gatctcgagc gcctcgtcca ggtactgctc gggtcggttg
      601 cggtaggtgg tccaggacat gccgttgatc acccacggca cgccggcctt ctcgagctgc
      661 tcagccaggt cagccggctg cgtgtaggtg cgccccttcg gcagcacgcc gcccgcggcg
      721 atcttcccgt tgatgtcggg cggcgggaac agcagcggca cgtcggcgta cacgccgacc
      781 ttgccggccg ccgagagatc ctcgacgaac gtccgctcct tgatcgactt gtaggtgacc
      841 ggatactgct tgtgggtccc cggcacggtc tcgaggatgt cgtagacccc gtggtcggcc
      901 gggtcgaccc cggtcaggaa cgacggccac gccgcccacg agtgggtggg caccgtcgag
      961 cgcaggacgc ccttctgccc ccggtcgatc agggcctgca ggttcggcag gacaccccgc
     1021 tcgagcaggg gttcgaggat gcgccagtcg gcgccatccc atccgatgag tagtactcgt
     1081 ggcatgaaag tcattcccta tcagaccaat gaaccggtga gtctacttga gctccgtacc
     1141 tgtcattctg tccgaacgag cggatcaatc gtccgcgaga tattgctcaa tcgtttgagc
     1201 catgaggccg tagtcggcga ggaaacgcgt cagttggcgc ttctcaattt cctgctcccc
     1261 aacgaacctt tgtggtccaa tgcggcgcct acatgcacca atgacgagct tcttccgcgc
     1321 tcgtgtgagt gacacgtata ggagtctccg cgcctcgtcg agatatgcac ccgtaccgtc
     1381 gcctgggatg acctcgtcct ccgcctgcaa cacaatgaca gtgtccgccg tcagtccttt
     1441 cgcgccgtgc attgtcgtga aggtgactcg cccttcaagc cgtggttggt actcgtcgac
     1501 cactatctcc cggagagcct ctggactgag cgccggatcc gagcgcagct caatcaagag
     1561 ttgatagacg agttcggaac gcaatgtgta gccatatacc tgcctgtgtc gttgccacgc
     1621 cgacaagaac tgcgcatcag gatggcctgc ttcccatccg gcaccatctg cctcgagcgt
     1681 ctcccaatca tcggcgagta gatcgagtgc gccgtccttc ccattcgata tctccttcac
     1741 cctgcgtcca agcagccggg ccagctcttc aatgacaatc caacgctcct cccagtcgcc
     1801 cgccacccgg accggggctg gcagacgacg cgccccgtgg agcatcagtt gtcgaagcgc
     1861 agaggagtga agggtcgaaa cccgcacgcg ctccccgcga tcaccgagct gctcaactag
     1921 gcgatcacgc atctcggcag ccgcagcccg actgaaggtc agagcttgga tgtctcctgg
     1981 tgacacaccc actacctcaa tgaggtactg gattcgtcga accagcacga aggtcttccc
     2041 ggttcctggc cctgcgagca acaccagatg gcctgaagca acaccagcgg cctcctgttg
     2101 ctcagttgat aggtcttcgt cccacgccat gtcatatctc tcgtcaggtg tcgtgccaat
     2161 tctctaccgt ctagcatgcc ttgttgttcg gctcatgttg cgacaagtgc gtcgaggctc
     2221 cgccagagcc acttcgagca cgccgggcag tcgaagtctc cgaactggcg cgcagctcat
     2281 cggccagccg ctgccaatac gacgtcggcc accttgcgaa agggactcgc gtagcgttcg
     2341 aacaacatcg tccactggtc ccagacggat cgcggcggcc acttcgccgc ctcatgcgcc
     2401 cgacctattt atcgaagacc tgcggacgtt cttccgt
//
LOCUS       DATITW010000532         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_707554, whole genome shotgun sequence.
ACCESSION   DATITW010000532 DATITW010000000
VERSION     DATITW010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707554"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2399)
                     /gene="treS"
                     /locus_tag="VLS86_07390"
     CDS             complement(<1..>2399)
                     /gene="treS"
                     /locus_tag="VLS86_07390"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HSO50356.1"
                     /translation="LWLLPFYPSPLRDDGYDTADYKAIHPSYGTMADFRQFLREAHKR
                     DLKVITELVINHTSDQHPWFQRARRAAAGSPERDFYVWSDDPERYKDARIIFQDTETS
                     NWSWDPVANAYYWHRFFSHQPDLNFDNPAVHNAVFDALDFWLDAGVDGLRLDAVPYLF
                     EREGTMCENLPETHDFLKKLRGRVDSKYEGRMLLAEANQWPEDAAAYFGEGDECHMSF
                     HFPLMPRLFMSLRLENRFPIIDILEQTPAIPETSAWAIFLRNHDELTLEMVTDEERDY
                     MYRVYASDPQARINVGIRRRLAPLLGHNRREIELMTALLLSLPGTPVLYYGDEIGMGD
                     NVYLGDRNGVRTPMQWSGDRNAGFSEANPQRLYFPVITDPEYHYESVNVQVQRDNPNS
                     LWWWMKRALSLRKGLPELSRGELEFLNSENPKVLTFLRTIGERSVLVVANLSRHTQPV
                     EIDLSRFEGVEPVELFGGTRFPAAGPNPYLLTIGPHDFYWFSLAAAETSREDLADRPR
                     VVLRGEMSGVFRARAQLERALVADIQGRRWFRSKAQTIRSGHLVERLEIPGTEAVVAF
                     LELEYVERDPETYAMPISLATGVDAERILLDTPGAVIAEVQSGSDLGVIYDASYDPSF
                     GFALISLAGSRRKVKTRDTMLYATPVAGCRALAGEVQDEQVRVGGVEQSNTSMIFGEK
                     LIMKLFRKIESGPNPEVEVGRFLTERTEFANTAPTCGVINVELRSQQAALAVVERFVP
                     SHGDAWSHMLGSLGLIFEQVAAHLEELGRPPPAVHPLQITDVELDGARPLMGVTLLET
                     A"
BASE COUNT          421 a          774 c          788 g          416 t
ORIGIN      
        1 aaggccgtct cgagcaaggt gacacccatc agagggcgcg ccccgtccag ttccacgtcg
       61 gtgatctgca gagggtgcac ggccgggggt ggccggccca gctcttcgag atgtgccgcg
      121 acctgctcga agatgaggcc cagtgagccg agcatgtgag accaggcatc gccgtgactg
      181 gggacaaagc gctccacgac ggcaagggcg gcctgctggc tccggagctc gacattgatc
      241 acgccgcagg tgggagcggt gttggcgaat tcggtccgct cggtgaggaa ccggccaacc
      301 tccacctccg ggttgggacc ggactcgatc ttgcggaaca gcttcatgat cagcttctcc
      361 ccgaagatca tcgaggtgtt cgactgctcg acacccccca ccctgacctg ctcgtcctgc
      421 acctcgcccg ccaacgcccg acagccggcg accggcgtcg cgtagagcat ggtgtctctc
      481 gtcttcacct ttctcctgct cccggcgagc gagatcaagg cgaatccgaa actcgggtcg
      541 tagctggcgt cgtagatcac ccccagatcg gatccggact ggacctcggc gatcaccgcc
      601 cccggggtgt cgaggaggat gcgctcggca tcgacaccgg ttgcgagcga gatcggcatc
      661 gcgtaggtct cagggtccct ctccacgtac tccagctcca ggaaggcgac cacggcttcc
      721 gtgcccggga tctcgagacg ctccaccagg tgaccgctcc tgatggtctg agccttgctc
      781 cggaaccagc gtcgaccctg gatgtctgcg accaaggccc gctccagctg ggcccgggcg
      841 cggaacacac cgctcatctc cccgcggagg acgacgcgcg gccgatccgc caggtcctcc
      901 cgggatgtct cggcggcggc caggctgaac cagtagaagt cgtggggccc gatggtcagc
      961 agatagggat tgggccctgc cgccgggaac cgtgtccctc cgaacagctc caccggttcg
     1021 acgccctcga atcgggacag atcgatctcg accggctggg tgtgacggga aagattggcc
     1081 accaccagga ccgatcgctc cccgatcgtc ctgaggaagg tcagcacctt ggggttctcg
     1141 ctgttgagga actccagctc gcctcgcgac aactcgggca accccttgcg cagggagagg
     1201 gcccgcttca tccaccacca caacgagttg gggttgtccc gttgcacctg gacgttgacc
     1261 gactcgtagt ggtactccgg atcggtgatc accgggaagt agagacgctg cgggttggcc
     1321 tcggagaacc cggcgtttcg gtcccccgac cactgcatcg gggtgcggac cccgttccgg
     1381 tccccgagat agacgttgtc ccccatgccg atctcgtctc cgtagtagag aacgggcgtc
     1441 ccgggcaacg aaagcagtag cgctgtcatc agctcgatct cccgccggtt gtggccgagg
     1501 agtggggcga gacggcgacg gatgccgaca ttgatccggg cctggggatc gctggcgtac
     1561 acccggtaca tgtagtcccg ctcctcgtcg gtgaccatct ccagagtcag ctcgtcgtgg
     1621 ttgcggagga atatggccca cgccgaggtc tcggggatgg cgggcgtctg ctccaggatg
     1681 tcgatgatcg ggaaccggtt ctccagccgc agcgacatga acagtcgggg catcagcggg
     1741 aagtggaacg acatgtgaca ttcgtcgcct tcgccgaagt aggccgcggc gtcctccggc
     1801 cactggttgg cctcggccag gagcatccgg ccctcgtact tcgagtccac ccgcccccgc
     1861 agcttcttca agaaatcatg ggtctcgggg aggttctcgc acatcgtccc ctcccgctcg
     1921 aagagatagg ggaccgcatc gagccgaaga ccgtcgacgc cggcatcgag ccagaagtcg
     1981 agcgcatcga acaccgcgtt gtggaccgcc ggattgtcga agttcaggtc gggctgatgg
     2041 gagaagaacc gatgccagta ataggcgttg gccaccgggt cccacgacca gttcgaggtc
     2101 tcggtgtcct ggaagatgat ccgggcgtcc ttgtacctct ccgggtcgtc ggaccagacg
     2161 tagaagtcgc gctctgggct gcccgcagca gcccggcgag cccgctggaa ccacgggtgc
     2221 tgatcagagg tgtgattgat caccagctcg gtgatcacct tgagatctcg cttgtgcgcc
     2281 tcccggagga actggcggaa gtcggccatg gtgccgtacg aggggtggat cgccttgtag
     2341 tcggcggtgt cgtagccgtc gtcccggagc ggcgagggat agaaggggag cagccacaa
//
LOCUS       DATITW010000533         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_587515, whole genome shotgun sequence.
ACCESSION   DATITW010000533 DATITW010000000
VERSION     DATITW010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587515"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1618
                     /locus_tag="VLS86_07395"
     CDS             <1..>1618
                     /locus_tag="VLS86_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014443185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HSO50357.1"
                     /translation="LEVDPSAGLDQAATEERLARFGPNAIPQETPPSVWTVALAQLSN
                     PMNIMLVIVAVASLFIGQVPTAVIVGALVTFNVLMGTNQELKARASVDALSQLQVRHA
                     RVRRSGAVEQIEATGLVPGDVVLLEAGDVVPADGRIVTSATLEVQEAALTGESAPVSK
                     DASPTALVDVALGDRTDMVFQNTLVTRGTAIVVVTSTGVSTQMGRIAGMVTAVERSKS
                     PLQQELDSLTKVFGFMAWGAVAVIAIAGLLRDQEIETLLLLCISTAIASIPTGLPTFV
                     QTMLSSGARHLADAKAVVKTLTDVETLGGTTAINSDKTGTLTMNAMTATTMLAGGEWY
                     KVEGSGYSKTGAILGVAGGAVPDFSRLGLGLTLCSDATVADDGSVIGDPTEAALVVLA
                     AKLGVDAEETRRALPRVAEVPFDSAYKYMATFHDRPEWLAPGYLRAPHLISVKGAPDV
                     VLDICDRALWHGEVRPIGEVRDEILSANRRLSEQGLRVLGFAGGDLEDAQAAAAMANP
                     MAAVSNLLFIALVGIIDPLRLEARDAVQVAL"
BASE COUNT          265 a          529 c          546 g          278 t
ORIGIN      
        1 ctcgaggtcg acccctctgc gggtctcgac caagcggcaa ccgaggagcg gctggcccgg
       61 tttgggccga atgcgatccc ccaggagaca ccaccaagcg tctggacggt cgcgctcgcg
      121 cagctgtcga acccgatgaa catcatgctc gtgatcgtgg cggtcgccag cctgttcatc
      181 ggccaggtcc ccaccgccgt catcgtggga gctctcgtca ccttcaatgt cctgatgggg
      241 accaaccagg agctcaaagc gcgagcaagt gtcgatgccc tgtcccagct ccaggtgcga
      301 cacgcccgtg tccgccggtc cggtgctgtc gagcagatcg aggcgaccgg actggttccc
      361 ggtgacgtgg tcctgctcga agccggcgat gtcgtccctg ccgacgggcg aatcgtgacc
      421 tcggcgacgc tggaggtgca ggaagcggca ctgaccggag agagtgctcc cgtctcgaag
      481 gacgcgtccc ctaccgcgct cgtcgatgtt gcgctgggtg accgcaccga catggtcttt
      541 cagaacactc tggtgacgcg gggaacggcc atcgtggttg tcacgtcgac cggtgtctcc
      601 acccagatgg gccggatcgc cgggatggtc accgccgtcg agcggagcaa atccccgctg
      661 cagcaagagc tggacagctt gaccaaggtc tttggattca tggcgtgggg agcggttgcg
      721 gtcatcgcga tcgcgggcct tctccgcgac caggagatcg agacactgct gctgctctgt
      781 atctcgactg ccatcgcgtc gattcccacc gggcttccga ccttcgtcca gacgatgttg
      841 tcctccggtg cgcgccacct cgccgacgca aaggctgtgg tcaagaccct caccgacgtc
      901 gagacccttg gtgggacgac agcgatcaac agtgacaaga ccgggacgct gacgatgaac
      961 gcgatgaccg cgaccacgat gctggccgga ggcgagtggt acaaagtgga ggggagcggc
     1021 tattccaaga ctggtgcgat cctgggggtg gccggaggcg cggtgcccga tttcagccgg
     1081 ctcggcctcg ggctgaccct ctgcagcgat gccacggtgg ccgacgacgg ttccgtcatt
     1141 ggcgacccga ccgaggcggc gctggtggtc ctcgccgcca agctgggggt agacgcggag
     1201 gagacgcgcc gagccctgcc ccgcgtcgcc gaggtcccct tcgactcggc ctacaagtac
     1261 atggcgacgt tccacgatcg tcccgagtgg ttggcgccgg gctacctgcg cgctcctcac
     1321 ctgatcagcg tcaagggcgc ccccgacgtc gtgctcgaca tctgcgaccg agccctctgg
     1381 cacggcgagg tccgccccat cggtgaggtg agagacgaga tcctgtcggc caaccggcgc
     1441 ctctcggagc agggtctgcg agtcctggga ttcgccgggg gtgacctgga ggacgcgcag
     1501 gctgcggccg ccatggccaa cccgatggct gccgtgagca atctgctctt catcgctctg
     1561 gtcgggatca tcgatccttt gcggctcgag gcacgagatg cggtgcaggt ggcactca
//
LOCUS       DATITW010000534         1679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_266618, whole genome shotgun sequence.
ACCESSION   DATITW010000534 DATITW010000000
VERSION     DATITW010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266618"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /locus_tag="VLS86_07400"
     CDS             <1..1523
                     /locus_tag="VLS86_07400"
                     /inference="COORDINATES: protein motif:HMM:NF019880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HSO50358.1"
                     /translation="QSVDFAEAIEILARRAGITLVRDPADAQRRGRRGAAVEAIRQAI
                     EVYHERLKKAPEAGPARAYLRNRGYDVGHIDQWKLGFAGVAWDTLTKELKAAGLEDRA
                     LVDAGLSRRGRQGLFDVFRGRLMFPIHDLRGDPVGFGGRKIDEIDRNATNNPDAKYVN
                     SADSIVYHKAQVLFGLDRARREISTEAPAVVVEGYTDVIAMHLAGIKTAGATCGTALG
                     DGHFELLRRFGEKVVLAFDSDEAGSKAALRGDELESPFRLDLDLRVALMPDGLDPADL
                     VQQGRGAELVASVAGARPWLERRIEHEVGRHDLSGPEGRARALHAAAEQVRRVNDDIA
                     RREYSRHVARLVGVDLDSVEAAVEGGRARARTGARVSNQPLDRMEAELLRVLLSDPSQ
                     VSGLAPEDFSDDRLRAAYLAVADGLAAVTAGQPVDFSGVADEGVQSLLRSLAMDERPL
                     PSGPEMLSRVKERRLDIEIADLQRLLAGMEPGTQSHSDNLRRLIALQQEKRSSAKM"
BASE COUNT          279 a          524 c          604 g          272 t
ORIGIN      
        1 cccagagcgt cgatttcgcc gaggccattg agatcctggc gcgacgcgcc gggatcaccc
       61 tggtgcggga ccctgccgac gcccagcgac ggggccgacg cggggcggcg gtggaagcga
      121 tccgccaagc gatcgaggtc tatcacgagc gcttgaagaa ggctccggaa gccggtccgg
      181 cgcgcgccta tctccgcaac cgcggatacg acgtcggcca catcgaccaa tggaagctgg
      241 ggtttgcggg cgtcgcctgg gacacactga ccaaggagct gaaggcggcc ggcctcgagg
      301 atcgggctct ggtcgacgcc ggtctcagcc ggcggggacg gcagggtcta ttcgacgtgt
      361 ttcggggacg tctcatgttc cctatccacg acctccgtgg cgacccggtg gggtttggtg
      421 gtcgcaagat cgacgagatc gaccgaaacg cgaccaacaa ccccgacgcc aagtatgtga
      481 actcggccga ctcgatcgtg taccacaagg cccaggtcct gttcgggctc gaccgggccc
      541 ggcgtgagat ctcgacggag gcgcctgccg tggtcgtcga gggctacaca gacgtgatcg
      601 ccatgcacct ggccgggatc aagaccgcgg gtgccacctg tggcaccgcc ctgggcgacg
      661 gacatttcga gctgctccgt cgatttgggg agaaggtcgt ccttgccttc gactccgacg
      721 aggccggctc caaggctgcg ttgcgtggtg atgagctcga gtcaccgttt cgcctcgacc
      781 tcgacctgcg ggtggcgctg atgcctgacg ggctggatcc tgcggacctg gtgcagcagg
      841 ggagaggcgc cgagctggtg gcctcggtgg ccggcgcccg cccttggctc gagcgtcgca
      901 tcgagcacga ggtcgggcgt cacgatctct ccggtcccga aggccgggcc cgggccctcc
      961 acgccgccgc cgagcaggtg aggagggtca atgacgacat cgccagacgt gaatacagca
     1021 gacatgtggc gaggctggtc ggtgtcgatc tcgacagcgt cgaagccgcg gtcgagggcg
     1081 ggcgggcgcg tgcccggacc ggcgcccggg tctccaacca gccacttgac cggatggagg
     1141 ccgagttgct ccgggttctg ctctccgatc cgtcccaggt gagtggtctc gcaccggagg
     1201 atttctcaga cgaccggctg cgtgccgcct acctggcggt tgccgacggt ctggcggcgg
     1261 tgaccgcggg ccagccggtc gatttctccg gcgtggccga cgagggagtg cagtcgttgc
     1321 tccgctcact ggcgatggac gagcggccgc tcccctcggg acccgagatg ttgtcccggg
     1381 tcaaggagcg aaggctcgac atcgaaatcg ccgatctgca gcgtttgctt gccggaatgg
     1441 agccgggcac ccaatcccat tccgacaact tgcggcggtt gatagcttta cagcaggaga
     1501 agcgatcctc cgcgaagatg tgaacgaaga gatcaacaac gtggtggccg ccctggtgaa
     1561 gcggggcaag catcgtggct ttctcacgat ggccgaggtg cagcaggagc tggaggatgc
     1621 cgaggcgtcg ggggaagcat tcgagaaggg ggccgacgcc ctgcgctccc aggggatga
//
LOCUS       DATITW010000535         1547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_587534, whole genome shotgun sequence.
ACCESSION   DATITW010000535 DATITW010000000
VERSION     DATITW010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587534"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VLS86_07405"
     CDS             complement(<1..430)
                     /locus_tag="VLS86_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSO50359.1"
                     /translation="MTVREIRVDPGAWDPVVSDADGAQLIVAGPGTGKTEFLVQRVAH
                     IVNSGQARRDQISLLTFSRRASADMRRRVGNALEGSGVPVDASTFHSLALRFLEAGNQ
                     GDRPIPLTPPEQVSLVAELLAAENSDDWPVTYRGILSTRGF"
     gene            complement(447..590)
                     /locus_tag="VLS86_07410"
     CDS             complement(447..590)
                     /locus_tag="VLS86_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50360.1"
                     /translation="MREMKKQETSGSRPEIDPFTDPVRYLMGLGIEAELVETREADLP
                     SAA"
     gene            662..>1547
                     /locus_tag="VLS86_07415"
     CDS             662..>1547
                     /locus_tag="VLS86_07415"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HSO50361.1"
                     /translation="MAKAVTPVLKPIGHEFDSPMYHEAVGQFDRVARLMGLDDNVADR
                     LRVPQKATVVSFPFRHDNYSEVETLFGYRVQHLTTMGPTKGGIRFAPDVDLGEVAALA
                     MLMTWKCSIVGLPFGGAKGGVRVGPFDLSRAELQRVTRRFTMEIINVIGPETDIPAPD
                     LGTNEQVMAWMMDTYSQHVGHSVPAVVTGKPPALGGSIARREATGRGIMFLLPQAAAS
                     RDLDPKDATVAIHGFGNVGRYAAIAGHQMGARVVAVTDISGAVYNPDGLDVDALAKWV
                     DENRFLDGYPDADHFEGDA"
BASE COUNT          270 a          479 c          519 g          279 t
ORIGIN      
        1 cgaagccccg ggtgctgagg atcccgcggt aggtgaccgg ccagtcgtcc gagttctccg
       61 ctgcaaggag ctcagcgacc agagagacct gttccggcgg ggtgagcggt atcgggcgat
      121 ccccctggtt gcccgcttcg aggaatctca gcgccaggga gtggaaggtg gaggcgtcga
      181 cgggcacacc ggacccttcg agggcgttgc cgacccgtcg tcgcatgtcg gctgatgcgc
      241 gccgggagaa tgtcagcagc gagatctggt cacgacgggc ctggccgcta ttcacgatat
      301 gggccacccg ttgcaccagg aactcggtct tgccggtccc cgggccggcg acgatcagct
      361 gggcgccgtc ggcatccgaa accaccgggt cccaggctcc gggatcgacc cggatctccc
      421 tgactgtcac ctcgcgatcg tagagatcag gcagcagagg ggagatcggc ttctcgggtt
      481 tccacgagct cggcttcgat cccgagaccc atcaggtaac ggaccgggtc ggtaaagggg
      541 tcgatttccg ggcgggaacc ggatgtctcc tgcttcttca tctctctcat gacagtcacg
      601 ctacgggccg cctgtgacag aaaatcagaa cggggcgttt ccggcatagg atgtgtgtcg
      661 tatggccaaa gccgttacgc cggtgctgaa gcccatcggt catgaattcg actcaccgat
      721 gtatcacgag gcggtcggcc aattcgatcg ggtggcacgg ttgatgggtc tcgatgacaa
      781 cgtcgcagac cggctccggg tcccacagaa ggcaacggtc gtcagcttcc cgttccgcca
      841 tgacaactac tccgaggtgg agaccctctt tggctaccgg gttcagcatc tgacgacgat
      901 ggggccgacc aaggggggga tccgtttcgc ccccgatgtc gacctcggtg aggtcgccgc
      961 cctcgccatg ttgatgacat ggaagtgctc gatcgtcgga ttgccgttcg ggggggccaa
     1021 aggtggggtt cgcgtcggcc cgttcgacct ttcgcgggcc gagctgcaac gtgtgacgcg
     1081 gcgattcacg atggagatca tcaatgtgat cggccccgaa accgacatcc ccgcgcctga
     1141 cctcgggacc aatgagcagg tgatggcctg gatgatggac acctactccc agcatgtcgg
     1201 ccactcggtg ccggcggtgg tcaccggcaa gcctcccgct ctgggcggga gcatcgcccg
     1261 aagagaggcc accggccggg gcatcatgtt tctcctgccc caggcagccg cctcgcggga
     1321 tctcgatccc aaggacgcga ccgtcgccat ccacggtttt ggcaatgtgg gccgctacgc
     1381 cgcgattgcc ggccaccaga tgggagcgcg ggtggtcgct gtgaccgata tctcgggggc
     1441 ggtgtacaac cctgacgggc tcgacgtgga tgcgttggca aagtgggtgg acgagaaccg
     1501 attcctcgat ggataccccg acgccgacca cttcgagggt gacgccg
//
LOCUS       DATITW010000536         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_306832, whole genome shotgun sequence.
ACCESSION   DATITW010000536 DATITW010000000
VERSION     DATITW010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306832"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..556
                     /gene="greA"
                     /locus_tag="VLS86_07420"
     CDS             80..556
                     /gene="greA"
                     /locus_tag="VLS86_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HSO50362.1"
                     /translation="MPEAPTFLTPAAYEKMRDELEHLKTDGRKEIEERIAEARSHGDL
                     RENADYDAAKNDQGLMESRIRKLEFLLNTAEVREVVDTGRVEIGSVVTVVDSDGDELD
                     YFVAPQENKVPGLHLASPTSPLGGALLGAAVGDKISYQAPAGTFAVTVKAVRPYEG"
     gene            676..2004
                     /gene="mgtE"
                     /locus_tag="VLS86_07425"
     CDS             676..2004
                     /gene="mgtE"
                     /locus_tag="VLS86_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HSO50363.1"
                     /translation="MADYLESHSEEWEALAGTYPGDAADVLEQLTEEDAAGLLAELDP
                     EDAAEILEQIAPELAAELVEEVPIAELSAAVSEMTGESAADLLFELDDELSESVLALM
                     DDEAEREIRRLLQYPPDTAGGLMTTEIAALPIGLTAGEAIERIRQLHDEYEDLSYVYV
                     VDDDRRLKGVVSFRDLVFARPGASLDEVMVPEPIAVHTQADREEVADLSQRYHLFGIP
                     VIDDLGRLLGMVTTDAVIETVQDEATEDFAAAVGAGVGETVYTDVGKSVASRAPWLVL
                     NLVLALFVAWVIEHQTGIIRREPVLAALMPIVATLGGSGGNQSLAVMIRSLATDDVPR
                     AQVPSIILRQAGVGILNGLAVAVAAGALTYGLIGAGVFDSNAEATQVAGVIALACLAN
                     LFVATLSGSAIPLVLRRLGLDPALAASILLTVITDVVGFGGFLLVAAALL"
BASE COUNT          351 a          684 c          708 g          340 t
ORIGIN      
        1 cgcgccgctg gcctccgggc ccatgcccgg caccccgacc cgtcgctagc cgcaaggcac
       61 aattagcctt tggcgaccga tgcccgaagc accgaccttt ctgaccccgg ccgcctacga
      121 gaagatgcgg gacgagctgg agcatctcaa gaccgacggt cgcaaggaga tcgaagagcg
      181 cattgccgag gcgcgctcac acggtgacct gcgggagaac gccgactatg acgcggcgaa
      241 gaacgatcaa ggcctgatgg aatcgcggat ccgcaagctg gagttcctgc tcaacaccgc
      301 cgaggtgaga gaggtggtcg acaccgggcg ggttgagatc ggaagcgtcg tcaccgtcgt
      361 cgactccgac ggcgacgaac tcgactactt cgttgctccc caggagaaca aggtgccggg
      421 actgcatctc gcctcaccga ccagtcccct gggaggtgca ctgctcggcg ccgcggtcgg
      481 agacaagatt tcctaccagg cgccggcggg gaccttcgcg gtgacggtca aagcggtgcg
      541 accatacgag ggctgacccc gccgttagcc tgacaccggt tcgccgtccg agggcttccg
      601 gtgagacttc gacttagacg tccccgtcaa cttgcaaagg atcttcttgc tgccgcgcgc
      661 cgcgaccgcg acgaggtggc tgactatctc gagtcgcaca gcgaggagtg ggaggccctg
      721 gccggtacct atccgggtga cgccgccgac gtcctcgagc agctgaccga ggaggacgca
      781 gccgggctgt tggcggagct cgaccccgag gacgccgcgg agatcctgga gcagatagcc
      841 ccggagctcg ctgccgaact ggtcgaggaa gtccccatcg ccgagctgag cgctgccgtc
      901 agtgagatga cgggagagtc ggccgccgac ctgctcttcg agctggacga cgagctgtcc
      961 gagagtgtcc tcgccctcat ggacgacgag gcggagcggg agattcgccg tcttctccag
     1021 tatccgcccg acacggccgg tggactgatg accaccgaga ttgcggccct tcccatcggc
     1081 ctgacagccg gcgaggcgat cgagcgaatc cggcaactcc acgacgagta cgaggacctc
     1141 tcctacgtct acgtcgtgga tgacgatcgg cgtctgaaag gggtggtgtc gtttcgcgat
     1201 ctggtcttcg cccgacctgg agcgagcctc gacgaggtga tggtgccgga gccgatcgcg
     1261 gtccacaccc aggcggaccg ggaagaggtc gccgacctga gccagagata ccacctgttc
     1321 ggcataccgg tcatagatga cctcggtcgc ctgctcggga tggtgaccac ggacgccgtg
     1381 atcgagacgg tccaggacga ggcgaccgag gatttcgccg ccgcggttgg tgccggcgtc
     1441 ggggagacgg tgtacaccga cgtcggcaag tcggtggcgt cgcgggcccc atggctggtg
     1501 ctcaacctcg tcctggccct cttcgtggcc tgggtcatcg agcatcagac cgggatcatc
     1561 cgacgcgagc cggtgctggc cgccctgatg ccgatcgtgg ccacactggg gggaagtggc
     1621 ggcaaccaga gcctggccgt aatgatccga tccctggcca ccgacgatgt gcccagggcc
     1681 caggtgccct cgataatcct caggcaagcc ggggtcggga tcctcaacgg gctcgcggtg
     1741 gcggtggcgg ctggagccct cacctacggc ctcatcgggg ccggggtctt cgacagcaat
     1801 gccgaggcga ctcaggtcgc cggggtcatc gctcttgcct gcttggcgaa cctgttcgtg
     1861 gccactctca gcggcagcgc catccccctg gtgctgcgac ggctggggct ggacccggct
     1921 ctggcggcct cgatcctgct cacggtgatc accgacgtcg tcggcttcgg cgggttcctg
     1981 ctggttgccg ccgccctgct ctgaggtcac ccgggccggg aagctctttc tcagctcccg
     2041 atggccagtt cgcctgagct ctcgagcggg ccccggaaat gaa
//
LOCUS       DATITW010000537         1638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_547578, whole genome shotgun sequence.
ACCESSION   DATITW010000537 DATITW010000000
VERSION     DATITW010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547578"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VLS86_07430"
     CDS             complement(<1..632)
                     /locus_tag="VLS86_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSO50364.1"
                     /translation="MALKLPIFMDAQSTTPADPRVVEVMLPYFTEKFGHPASRNHPFG
                     WEAEAAVDRAREQVGALIGARDPKEVIFTSGGTESINLAIKGVVEMYREQGNHIVTTA
                     IEQRAGLDVCRRLERHGVEVTYVPVQPDGLVSVDEIRKAITDKTILISVMFANNEIGT
                     IQDIAAIGKLAKEKGILFHTDATQAAGKIPIDVESTGIDLLSCTAHLMYGP"
     gene            complement(623..1081)
                     /locus_tag="VLS86_07435"
     CDS             complement(623..1081)
                     /locus_tag="VLS86_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSO50365.1"
                     /translation="MLRFTKRADYGLMAIHYIAIQETLGPVSAKRIAEQVHIPAELLA
                     KILQRLAKRGLIVSQNGPKGGYALAQRPAQISVGDVIRALEGPVRIVSCMEEPSDCPQ
                     LSHCSLRRPVQKLQAAMSQMLDGMSLAELTSDDVPEMLSIAGVKESDRWR"
     gene            1216..>1638
                     /locus_tag="VLS86_07440"
     CDS             1216..>1638
                     /locus_tag="VLS86_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HSO50366.1"
                     /translation="MTDHPEFESPLYHTTVAQLDSVAGRIGLEADIHERLRRPRRALV
                     VSVPVRMDTGKTEVFMGYRVHHNTALGPTKGGLRYDLDVNLGEVTALAMLMSWKCALM
                     GLPYGGAKGGVRCDPRALSLREREGLTRRYTAEIILLIG"
BASE COUNT          257 a          543 c          525 g          313 t
ORIGIN      
        1 gggccgtaca tcaggtgggc cgtgcacgac aggaggtcaa tgcccgtgct ctcgacgtcg
       61 atcgggatct tgcccgcggc ctgggtggcg tcggtgtgga agaggatccc cttctccttg
      121 gccagcttcc cgatggccgc gatgtcctgg atcgtcccga tctcgttgtt ggcgaacatg
      181 acggagatca ggatggtctt gtcggtgatg gctttgcgga tctcgtcgac ggacaccagc
      241 ccgtcgggct ggacgggcac gtaggtcacc tcgaccccat ggcgctcgag gcgccggcac
      301 acgtccaggc ctgcccgctg ctcgatggcc gtggtgacga tgtggttgcc ctgctcccgg
      361 tacatctcga ccacgccctt gatggcgagg ttgatcgact ccgtgccgcc cgaggtgaag
      421 atgacctcct tggggtcccg ggctccgatc agggccccga cctgctcgcg agcgcgatcg
      481 acggcggcct ccgcctccca gccgaaggga tggttgcgac tggccgggtg cccgaacttc
      541 tcggtgaagt acgggagcat cacctcgacg acgcgtgggt ccgccggcgt ggtcgactgc
      601 gcgtccatga agatgggaag tttcaacgcc atcggtcgct ctccttgacg cccgcaatcg
      661 acagcatttc aggcacgtcg tcgctcgtca gctcggccag gctcatgccg tccagcatct
      721 gggacatggc ggcttgaagc ttctggaccg gcctccggag actgcagtgc gagagctgcg
      781 gacagtcgct cggttcctcc atgcagctca cgatgcgcac cggcccctcc agcgcccgga
      841 tcacgtcgcc gaccgagatc tgggcggggc gctgggcgag cgcgtatcca cctttggggc
      901 cgttctggct gacgatcagg cctcgttttg ccagacgctg aaggatttta gccagcaatt
      961 ccgccgggat gtgaacctgc tcagcgatgc gcttggcgct caccggcccg agggtctcct
     1021 ggatggcgat ataatgaatc gccatgagtc cgtagtcggc tcgcttggta aagcggagca
     1081 ttcattggcc ccttgcgtgc ctctcctggc cacccgtctc gatccaacgt cctgtccggc
     1141 gaccattccg atcgccgacc atttgagtcg tagtctggca cttccgagat ctcgagtcaa
     1201 caggaaagga cggagatgac cgatcacccc gagttcgagt cgcccctgta ccacacgaca
     1261 gtggcccagc tcgacagcgt ggctggccgc atcggcctcg aggctgacat tcacgagcgg
     1321 cttcgccgcc ctcgccgagc cctcgtggtg tcggtccctg tccggatgga cacgggcaag
     1381 accgaagtct tcatgggcta tcgtgttcat cacaacaccg cgctcggccc gaccaagggc
     1441 ggtctccgct atgatctgga cgtcaatctg ggcgaggtga cggccctggc catgctgatg
     1501 agctggaagt gcgccctcat gggcctgccc tacggaggag ccaagggcgg cgttcgctgc
     1561 gacccccgcg ccctctccct ccgggagcgc gaggggctca cacggcgcta caccgccgag
     1621 atcatcctcc tcatcggg
//
LOCUS       DATITW010000538         3169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_186512, whole genome shotgun sequence.
ACCESSION   DATITW010000538 DATITW010000000
VERSION     DATITW010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186512"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VLS86_07445"
     CDS             complement(<1..224)
                     /locus_tag="VLS86_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HSO50367.1"
                     /translation="MPKKVLSTPSAPKAMGPYSFATEGGGLVFLSGQVALDPATGERR
                     PDDVEVQARQVMDNIGVILGDLGMAYEDIV"
     gene            complement(291..653)
                     /locus_tag="VLS86_07450"
     CDS             complement(291..653)
                     /locus_tag="VLS86_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HSO50368.1"
                     /translation="MTQILTIKSNKPEKTVIVTDFAVTKIGELIAAEGEPGLALRMAV
                     RPGGCSGFSYEMYFDSQIDDTDIVEDYGDVKVVVDPQSAEMVKGSTLDYKDGLMGAGF
                     AIENPNVSRTCGCGNSFS"
     gene            750..1886
                     /locus_tag="VLS86_07455"
     CDS             750..1886
                     /locus_tag="VLS86_07455"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HSO50369.1"
                     /translation="MVTRDDVLKTLEGVRDPELGGNVVELGMISAVDIDGSTVDIGLA
                     LTIAECPMRNQIESDTLRRVQSMVGVEQVNLRTTAMTKKQRAGLMSVARAKARESAEP
                     TQVSPTTRVIAIGSGKGGVGKSSISVNIAVAMAASGFRVGLLDADIWGFSTPRMLGVD
                     ERLVASDETKLIIPVEAHGVKLVSTGLIIETEETALMWRGLMLSKALEQFLKQVEWGE
                     LDYLIIDLPPGTGDIQMALTRMLPQAEMVVVTTPQRAAQKVAIRVADMARRSHMPIVG
                     VIENMAGLACDCGRVHTVFGEGGGQELADDLGVPLIASIPLDPEVVPGGDDGRPVVGT
                     GTGSAAGIALRAAAETVVRLVPPAELETCTGRIAKILTELELPA"
     gene            complement(1927..2397)
                     /locus_tag="VLS86_07460"
     CDS             complement(1927..2397)
                     /locus_tag="VLS86_07460"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HSO50370.1"
                     /translation="MTTPPQFPTEQPQGTPANLAPWGTRAIGLLIDFLPAFILQAVFY
                     PIGGLRYLASVAVFLYGYLYLGYFDGLTGQTPGKAIMGTRVVNVQGELIGTGNGIARK
                     FAHILDSIVCLIGWFLPLFDAKRQTIADKVLNTYVVEGVEKKAFSVDLWMPPKT"
     gene            complement(2646..>3169)
                     /locus_tag="VLS86_07465"
     CDS             complement(2646..>3169)
                     /locus_tag="VLS86_07465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSO50371.1"
                     /translation="ARNATTTTTPAPGTTEASPGESTTTAPDTTETTSPPPPPSGGGG
                     TCPVAGAVSFTDSWGAPRSGGRAHQGVDMIAARNTPVVAIYSGTIRRISSGNLSGLSV
                     WLRAGNGDTFFYAHLESYGDISVGQSVDEGEVIGYNGSSGNAPSWLPHLHFEYHPGGG
                     SAVNPYPLVRSLC"
BASE COUNT          568 a          975 c         1079 g          547 t
ORIGIN      
        1 ttcacgatgt cctcgtaggc catgccgagg tcgccgagga tgaccccgat gttgtccatg
       61 acctgacggg cctgcacttc gacgtcgtcc ggccgacgct cacccgtggc cgggtccaga
      121 gcgacctgcc cgctcaggaa caccaaccca ccgccttctg tggcgaagga atacgggccc
      181 atcgccttgg gcgcggacgg ggtcgaaagg actttcttgg gcaaaaggag agctcctggt
      241 tgataccagg ctgagggtac ccctgggggc gacccaaccc tcaagccccg tcaggagaag
      301 ctgttgccgc agccgcaggt gcggctgacg ttggggttct cgatggcgaa cccggccccc
      361 atcagaccat ccttgtagtc gagggtcgag cccttgacca tctcggcgct ctgggggtcg
      421 acgaccactt tcacgtcgcc gtagtcctcg acgatgtcgg tgtcgtcgat ctggctgtcg
      481 aagtacatct cgtaggagaa cccgctgcaa ccgccgggac gaaccgccat gcggagggca
      541 agaccgggct cgccttcggc cgcgatcagc tcgccgatct tggtgacggc gaaatcggtg
      601 acgatgaccg tcttctccgg cttgttggac ttgatagtga ggatctgtgt catcttccct
      661 ggaacctggt tgcctgccgt caatagtaac cgcagatgag cctatggcca ttcccactac
      721 atggatagtg gaaattacca tgggccgtga tggtcacacg tgacgatgtc ctgaagaccc
      781 tggaaggggt cagagaccct gagctcggcg gcaatgtcgt cgagctgggg atgatcagcg
      841 ccgtggacat cgatggctcc accgtcgata tcggtctggc actgaccatc gccgagtgcc
      901 cgatgaggaa ccagatcgag agcgacaccc tgcgccgggt tcagtccatg gtcggggtcg
      961 agcaggtcaa cctccgcacc acggcgatga ccaagaagca gcgggcgggt ctgatgtcgg
     1021 tggcccgggc caaggcgaga gagagcgcag agcccaccca ggtctcgccc accacacggg
     1081 tgattgcgat cggatcggga aagggcggag tcgggaagtc gtcgatctcg gtcaacatcg
     1141 cggtggcgat ggcggcgtcc ggcttccggg tcggtctcct cgatgccgac atctggggat
     1201 tctcgacccc gcggatgttg ggtgtcgatg agcgtctcgt ggcgagcgac gagaccaagc
     1261 tgatcatccc ggtcgaggcg cacggggtga agctggtatc caccggtctc atcatcgaga
     1321 ccgaagaaac cgccctgatg tggcgaggtc tgatgctgtc caaagcgctg gagcagttcc
     1381 tgaagcaggt ggagtggggc gagctcgact acctgatcat cgacctgccg ccgggaaccg
     1441 gcgacatcca gatggctctc acccggatgc tgccccaggc agagatggtc gttgtgacca
     1501 ccccgcagcg agcggcgcag aaggtggcga ttcgggtcgc cgacatggcg aggagatcgc
     1561 acatgccgat cgtcggggtc atcgagaaca tggccgggct cgcctgcgac tgcgggcggg
     1621 ttcacaccgt cttcggggag ggcggtgggc aagagctcgc agacgacctc ggcgtgccac
     1681 tcatcgcatc gatccctctc gaccccgagg tggtgcccgg gggcgacgat ggcagaccgg
     1741 tcgtcggcac cggcaccggg tcggccgccg ggatcgccct gagagccgca gccgaaaccg
     1801 tggtccggct ggttccccca gccgagctgg agacctgcac cggtcgcatc gccaagatcc
     1861 tcaccgagct cgaactgcct gcctgagccg gcgcccgcgc gttggggcgg gccccgcacc
     1921 cgggtgtcag gtcttgggcg gcatccagag atcgaccgag aacgccttct tctcgacccc
     1981 ttccaccaca taggtgttga gcaccttgtc ggcgatggtc tgccgcttgg cgtcgaacaa
     2041 gggcaggaac caaccaatga gacagacgat ggagtcgagg atgtgggcga atttgcgggc
     2101 gatcccgttc ccggtgccga tcagctcacc ctggacgttg accacccggg tccccatgat
     2161 cgccttgccg ggcgtctgcc cggtgagacc gtcgaagtag ccgaggtaca ggtagccgta
     2221 gaggaagact gcgacgctag ccaggtagcg caaaccaccg atcggataga acaccgcctg
     2281 gaggatgaat gccgggagga agtcgatgag gagaccgatg gcgcgggtac cccacggagc
     2341 cagattggcc ggtgttccct ggggctgctc ggttgggaac tgcggtgggg tggtcactga
     2401 ttctcctcct cggggtctgt tcccgcattg aagcacctgc gacggcgcct ccgggcgcgg
     2461 atcgcggaga acttgggccg atccggccct ggaatggact agtcaaaacg agcttctcac
     2521 caagggtcat ttactaacca cccagagtgg tcgactatgt gttcagaacg tgcctcgagg
     2581 gcgtggggga taggggctgg agaggctgga tcggaagagg acccgcaggg gtcctcttcc
     2641 gcgtctcagc agagtgagcg caccaacggg tagggattga ccgccgagcc accgccgggg
     2701 tgatactcga agtggagatg gggcagccag gacggcgcat tccccgacga cccgttgtac
     2761 ccgatcacct caccctcgtc gacactctgc ccgaccgaga tgtccccgta gctctcgaga
     2821 tgggcgtaga agaaagtgtc gccgttcccg gcgcggagcc agaccgagag accactcaga
     2881 ttgccgctgg agatgcgacg gatcgtgccc gagtatatgg cgaccaccgg cgtgttcctc
     2941 gccgcgatca tgtccacgcc ctgatgggca cgtccccccg agcggggcgc tccccagctg
     3001 tcggtgaacg agaccgcccc ggccaccggg caggtcccac cgcccccgga cggtggtggt
     3061 ggtggtgagg tcgtctcggt ggtgtccggc gcggtcgtag tcgactcacc tggcgaggct
     3121 tcggtggtac ccggtgcggg tgtggtggtc gtggtggcat tgcgcgccg
//
LOCUS       DATITW010000539         1603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_266829, whole genome shotgun sequence.
ACCESSION   DATITW010000539 DATITW010000000
VERSION     DATITW010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266829"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VLS86_07470"
     CDS             <1..217
                     /locus_tag="VLS86_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50372.1"
                     /translation="ATEHHSSSAGEMLAKSIASVTGGRVEGRASALLKETSAPAVVVS
                     RAKLDEEVGLAVVDGITLFFAHAAATR"
     gene            complement(207..1058)
                     /locus_tag="VLS86_07475"
     CDS             complement(207..1058)
                     /locus_tag="VLS86_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HSO50373.1"
                     /translation="MSTRRSGLGRGLDALIPTTPTPESSGFRMVPVEAIVANPDQPRT
                     HFDDDTLEELAASMREVGVLQPIVVTETGDGYVLIAGERRWRAAKRAGMTAVPAVVRE
                     ATGSSTLVEALVENVQRQDLSPLEEANAYRHLLENYGMTQDEVADRVGKSRPTVSNTL
                     RLLQLPPAIQALVDNGQLSAGHVRPLVGMEDVAYATHLAERAATEGWSVRQMEEAVRT
                     RKALESKSSTRVRALRPVEIIELEKRLGERLGAQVKIDYRNEKGKLEIKFGSLADLER
                     IYRSFTA"
     gene            complement(1061..>1603)
                     /locus_tag="VLS86_07480"
     CDS             complement(1061..>1603)
                     /locus_tag="VLS86_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HSO50374.1"
                     /translation="VRDLFVVPATIDLAGAEIEMVSLFSRETRLRNAIQASGDGHDIV
                     LIDCPPSLGLLTVYALTAADEVLIPIQCEYYALEGLSQLLRNVQLVTTNLNPNLKVGG
                     VVLTMYDGRTKLSKDVAQQVRDHFGDITYRSVIPRSVRLSEAPSYGEPIEAFDPMSRG
                     AIAYRHLGKEFRERHGMKEK"
BASE COUNT          286 a          520 c          499 g          298 t
ORIGIN      
        1 cgccaccgag caccacagct cctccgccgg cgaaatgctg gccaaatcga ttgcctcggt
       61 caccggaggc cgggtcgagg gtcgcgccag cgccctgctc aaggagacca gcgcccccgc
      121 ggtggtcgtc tccagggcca agctcgacga ggaggtcggg cttgcggtgg tcgacgggat
      181 cactctcttc ttcgcccacg cagcagctac gcggtgaaac tgcggtagat ccgctccagg
      241 tccgccaggg agccgaactt gatctcgagc ttccccttct cgttgcggta gtcgatcttg
      301 acctgtgccc ccagccgctc accgagtcgt ttctccaact cgatgatctc gacgggtcgg
      361 agggcccgga cccgggtcga ggacttgctc tcgagcgcct tgcgggtccg taccgcctcc
      421 tccatttgtc ggacactcca gccctcggtg gcagcccgct ccgcgagatg ggtggcatag
      481 gcgacgtcct ccatcccgac caggggtcga acgtggcccg ccgagagctg gccgttgtcg
      541 acaagagcct ggatcgccgg cgggagctgg aggagacgaa gcgtgttgga aaccgtgggt
      601 cgggacttgc ccacccggtc cgccacctcg tcctgcgtca ttccataatt ttccaggagg
      661 tgacgatagg cgttggcctc ttcgagaggg ctcaagtcct gacgttgcac gttctcgacc
      721 agggcttcga ccagggtcga ggacccggtc gcctcacgga ccaccgcggg gaccgcggtc
      781 atccccgccc gtttcgcggc cctccatctt ctctcgccgg cgatcaggac atacccgtcg
      841 ccggtctcgg ttaccacgat cggctggagg acgccgactt cccgcatcga cgccgccagc
      901 tcctcgaggg tgtcgtcgtc gaaatgagtc cgtggctggt cgggattggc gacgatcgcc
      961 tccaccggga ccattctgaa gccgctcgat tcgggggtgg gggtggttgg gatcagggca
     1021 tccaagcccc ggccgagacc ggatcgtcgt gtcgacatcg ctatttctcc ttcatcccat
     1081 gacgttcccg gaactccttg cccagatgtc ggtaggcgat cgccccccgg ctcatcggat
     1141 caaaggcctc gatcggctcc ccataggagg gagcctcgga gagacgcacc gatcggggga
     1201 tcacactgcg ataggtgata tcaccgaaat ggtcacgaac ctgttgcgcc acgtccttgg
     1261 agagcttggt ccgaccgtcg tacatggtga gcaccactcc cccaaccttc aagttggggt
     1321 tgaggttggt ggtgaccaat tgcacgtttc gcagcaactg agagagaccc tccaatgcgt
     1381 agtactcgca ctggatcgga atcaacacct cgtcggcggc ggtgagtgcg tagacggtga
     1441 gcaaccccaa cgacggggga cagtcgatga ggacgatgtc atgaccgtca cctgaagcct
     1501 ggatggcatt gcggagcctg gtttcacgtg aaaacaagga aaccatctcg atttcggcac
     1561 cggcaagatc gatggtggcc ggcaccacga acaaatcccg aac
//
LOCUS       DATITW010000540         3605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_587796, whole genome shotgun sequence.
ACCESSION   DATITW010000540 DATITW010000000
VERSION     DATITW010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587796"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VLS86_07485"
     CDS             complement(<1..233)
                     /locus_tag="VLS86_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50375.1"
                     /translation="MAFWRRSGRAPGQPVAADPDPAGAGGGALEVETERTVEIRHLLS
                     KLATDLGLPATQPVGTPSGDPDFEPLVSVRVPV"
     gene            complement(245..988)
                     /locus_tag="VLS86_07490"
     CDS             complement(245..988)
                     /locus_tag="VLS86_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50376.1"
                     /translation="MKHETAVRAQEVTVRFRPFVERKPTLRKTLANLRSRERETVTAL
                     DQVSFEVSKGEAFGIVGRNGAGKSTLMRVVAGTLRPDGGRVVTYGRTSTLLQLGVGFN
                     NELSGARNVYLGGLAAGLRRTTIDDIFDDIVDYAELEEAIDRPIKTYSSGMVSRLAFS
                     IGMQLEPDILLLDEILSVGDEAFRTKSEAAMQELLDRSGTIVFVSHNLSKVADFCDRA
                     IWLERGVVRHQGPAAEVVEHYRAAVAAGR"
     gene            complement(985..1830)
                     /locus_tag="VLS86_07495"
     CDS             complement(985..1830)
                     /locus_tag="VLS86_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO50377.1"
                     /translation="MAVTPPTGIFYNARRLRTYFTDLWNRREFAWFLAMGNLKARNAS
                     TALGLLWWVLNPLLLGLVYFFVFGVLFGGGNRPDDYIAYLLSGMFAFHFTNQSMSGGA
                     NAILQNAKLLANLKFPSLVLLISSLIESSVGFLASLVVFYAITWPILGVHPSRFTLLM
                     VVVFPLHILFNLGLSAIVARLAVPFRDINNFIPYINRMWLYLTPIIWPLTLLDDAGPT
                     LTRVVELNPMFHFIGLYRTALIGYPGYPFQLDDLLVSLAWAVAIFLIGVAGFIRYETR
                     MVRYL"
     gene            1911..2423
                     /locus_tag="VLS86_07500"
     CDS             1911..2423
                     /locus_tag="VLS86_07500"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HSO50378.1"
                     /translation="MVAQAISAVALALLAASGLAKLLDPDPTRGAMAAARLPSSRWIA
                     RGLGALEIAAAVVGLVLAGVWLAPAATLYAGFFLFTLVAVQQRLPIQSCGCFGRDDTP
                     PTVLHVAFNLVSAGALTYSAVMGLPAVPWDAPPLDVLVYLSFALLGAYLAYLLLAQLP
                     RTLNMTTTTT"
     gene            2420..>3605
                     /locus_tag="VLS86_07505"
     CDS             2420..>3605
                     /locus_tag="VLS86_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HSO50379.1"
                     /translation="MSTRLVAAASRALDTHTSRRGFLRRAAMVGTALVAAPAAYVLRP
                     GSAYAAVVPSQCGSGARCNDGWTDFCANVHGVNTCPPGNVVAGWWRAEGNIYCDGNSR
                     YYMDCNAASCGGCSCGGSGTCSDSCVDCNCHCNHDRCAERKVCCTRFRSGQCNRHKPC
                     LGPITCRVVTCIPPWEWDSSCTRTDARSQATYNHNAPGLHPDTTAKGPYPARLAVLAG
                     ATWQLRNSLSTGGANQTFAFGAAGDVPVMGDFSNSGVRTVGTVRNARHGVAGATTLEW
                     YLRQVEGPGDPDLVVKYGQPGDIPVVGDWNGNGVHTIGVVRGNRWLLRNSNRAGDPDY
                     DFVFGEPGDIPVVGDWNGAGRTWIGVVRGDRWILRSSLSAGAPHFDFTFDGSGYPVTG
                     DWN"
BASE COUNT          655 a         1190 c         1158 g          602 t
ORIGIN      
        1 tgcaccggca cccgcacgga caccaggggc tcgaagtcgg gatctccgga cggtgttccg
       61 acgggctgcg tcgcgggcag gcccagatcg gtggcgagct tggaaagcag atgcctgatt
      121 tcgacggtcc gctcagtttc gacttcgagc gcccctcccc cggcacccgc cggatcagga
      181 tcggcggcga cgggctgccc cggtgcccgg ccacttcttc gccagaatgc caaagggtgt
      241 ccgctcaccg accggcggca accgcggctc gatagtgctc gaccacctcg gcggccgggc
      301 cctggtgccg gaccacgccc ctctccaacc agatcgcccg atcacagaag tcggcgacct
      361 tgctcaggtt gtgcgacacg aacacgatcg tgcccgagcg atccagcagc tcttgcatcg
      421 cagcttcgct cttggtccgg aaggcctcgt ccccgacgct gaggatctcg tcgagcagga
      481 ggatgtcggg ttcgagctgc atcccgatcg agaacgccag acgagacacc attcccgacg
      541 agtaggtctt gatcgggcga tcgatggcct cctccagctc ggcataatcc acgatgtcat
      601 cgaagatgtc gtcgatggtg gttcgccgga gccctgccgc caggccaccc aggtagacgt
      661 ttctcgcacc ggacagctcg ttgttgaagc caacccctag ttggagcaac gtcgaggtcc
      721 ggccgtaggt caccacccgc ccgccgtctg gtctcagggt cccggccacg acccgcatca
      781 aggtgctctt ccccgccccg ttcctgccca cgatcccaaa cgcctcgcct ttgctgacct
      841 cgaagctgac ctgatcgagg gcggtcaccg tctctctctc ccggctgcgg agattggcca
      901 gcgtcttgcg caatgtcggt ttgcgctcga caaaagggcg aaaccggacc gtcacttcct
      961 gggccctgac tgctgtctcg tgcttcacag gtagcgaacc attcgcgtct cgtacctgat
     1021 gaacccggca accccgatga ggaagatggc caccgcccag gccaatgaca ccaacaggtc
     1081 atcgagttgg aacgggtacc cgggatagcc gatcaaggca gtgcgataga gcccgatgaa
     1141 atgaaacatc gggttcagct cgaccactct cgtcaaggtg ggaccggcgt cgtcgaggag
     1201 agtgagcggc cagataatgg gtgtcaggta gagccacatc cggttgatat aggggatgaa
     1261 gttgttgatg tcacgaaacg gcacggcgag acgggccacg atcgcgctca gaccgaggtt
     1321 gaacaggatg tgaagcggga acaccaccac catgagcagc gtgaaccgcg aggggtgaac
     1381 tccgagaatc ggccaggtga tggcgtagaa caccaccaga gaagccagga aacccacact
     1441 cgactcgatg aggctggata tgaggagcac cagactgggg aacttcagat tggccagcag
     1501 cttcgcgttc tgcaggatgg cattggcgcc ccccgacatc gactggttgg tgaaatggaa
     1561 ggcgaacatc cccgagagca ggtaggcgat gtagtcgtcg ggccgattgc ctccgccgaa
     1621 caagacgccg aacacgaaga agtagacgag acccagcagc agggggttga gcacccacca
     1681 caacagacca agggcagtgg aggcgtttct cgccttgagg ttccccatgg ccaggaacca
     1741 ggcgaactcg cggcgattcc acagatccgt gaagtaggtc cgaagccggc gtgcgttata
     1801 gaagatcccg gttggaggcg tgacggccac ggaggttgat tgtatggccg aggaatcgcc
     1861 aaatcccact tcatgtttgt cctggccggg accagaacta cgctgccgga gtggttgccc
     1921 aagccatatc ggcagtcgcg ctggccctgc tggcagccag cgggctggcc aagctgctcg
     1981 atcccgatcc aacgagaggg gccatggcgg ctgcccgact cccctcgtcg cgctggatcg
     2041 cgcgcggtct cggagcgctc gagatcgccg ctgccgtggt cggcctggtg ctcgccgggg
     2101 tctggctggc tccggctgcc accctctatg ccgggttctt cctcttcacc ctggtcgcgg
     2161 tgcagcaacg gctcccgatc cagagttgtg ggtgttttgg tcgggatgac acaccgccca
     2221 cggtgctcca cgtggcgttc aacctggtct cggcgggggc gctgacctac agcgcggtca
     2281 tgggcctgcc ggcggttccg tgggatgccc cacccctcga cgtcctcgtc tacctctcgt
     2341 tcgccctgct cggggcatat ctggcctatc tcctgctggc gcagctcccc cgcaccctga
     2401 acatgacgac tacgaccaca tgagcaccag actcgtggcg gcggccagcc gcgccctcga
     2461 cacccacacc tcgcgacggg ggttcctgcg tcgggcggcg atggtcggca ctgccctggt
     2521 tgcagcgccg gccgcctacg tgctgcgccc tggcagcgcc tacgccgcgg tcgtgcccag
     2581 ccaatgcggc tcgggagcaa ggtgtaacga cggttggaca gacttctgcg ccaacgttca
     2641 cggagtcaac acctgcccac cgggcaacgt cgtcgccgga tggtggcggg cggagggcaa
     2701 catctactgc gacggcaaca gccggtacta catggactgc aacgcggcga gttgcggggg
     2761 atgcagctgt ggtggctcgg ggacctgtag cgacagctgc gtcgactgca actgccactg
     2821 caaccacgac agatgtgcag agcggaaggt gtgttgcacc aggttccgct ccgggcaatg
     2881 caaccggcac aagccatgcc tcgggcccat cacgtgccgg gtggtgacct gcatcccacc
     2941 ctgggagtgg gactcgtcat gcaccagaac cgacgcgcga tcacaggcga cctacaacca
     3001 caacgccccc gggctccatc ccgacaccac ggccaagggg ccctacccgg cccgtctggc
     3061 ggtcctggcc ggggcaacct ggcagctccg caactcgctg agcacgggcg gcgccaacca
     3121 gacgtttgcc ttcggggccg ccggcgatgt cccggtcatg ggcgacttct ccaactccgg
     3181 cgtccgcacc gtgggaactg tgagaaacgc gcggcacggc gtggcgggcg ccaccacgct
     3241 cgagtggtat ctacgccagg tcgagggccc cggggatccc gatctcgtgg tcaagtacgg
     3301 ccagcccggc gacatcccgg tggtcggaga ctggaacggc aacggggtgc acaccatcgg
     3361 ggtggtccgt ggcaaccgtt ggctgctgag gaactccaat cgcgccggcg accccgacta
     3421 cgacttcgtg ttcggtgagc ccggtgacat cccggtggtc ggagactgga acggcgccgg
     3481 tcgcacctgg atcggtgtgg tgcgcggcga tcgttggatc ctccgcagct cgctctcggc
     3541 cggcgcccca catttcgact tcaccttcga cggatcgggg tacccggtca ccggtgactg
     3601 gaacg
//
LOCUS       DATITW010000541         1755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_507209, whole genome shotgun sequence.
ACCESSION   DATITW010000541 DATITW010000000
VERSION     DATITW010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507209"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VLS86_07510"
     CDS             complement(<1..669)
                     /locus_tag="VLS86_07510"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HSO50380.1"
                     /translation="MDRRHQTWATSERLVPRRFVRPFVRFTQIEASSGIVLLAAAIAA
                     LVWANSAFSDTYFQVLETHLSVQFGPFDFDESLHHLINDGLMAVFFFVVGLEIKRELI
                     LGDLRDRRAAALPVMAALGGMIVPALIYIALTAGTGGEAVRGWGIPMATDIAFAVGEV
                     ALLGSTVPGGAKLFLLALAIADDIGAIAVIAIFYTDELNVAFLAMALGGLALVWAAQK
                     VGVRS"
     gene            complement(669..1697)
                     /gene="fbaA"
                     /locus_tag="VLS86_07515"
     CDS             complement(669..1697)
                     /gene="fbaA"
                     /locus_tag="VLS86_07515"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HSO50381.1"
                     /translation="MPIATPQKYLEMLDGARNGGFALPAINVTSSSTVIAAMEGLSQA
                     ASDGILQVSYGGAEFASGQSNKNIPIGAKALSEFAGVVAEQYQVNVALHTDHCPPDRV
                     DGYLRPLIEESLKRKTEGKGPLFQSHMLDASNLPMEENLALASELLGLCAEADIVLEL
                     EIGIVGGVEDATDNEDVDRAKLYTSPEDMVRVSEVPGVFDRGRYLLAAVFGNVHGVYK
                     PGAVRLEPTILRDGQAAVAERYGDEARHYLVFHGGSGSSIEEIHETLAYGVVKMNVDT
                     DCQYAYTRPIVDHVMKSYDGVLKVDGEVGDKKAYDPRSYLKKAENGMSARVVEAAEQL
                     LSAGKTIG"
BASE COUNT          313 a          578 c          541 g          323 t
ORIGIN      
        1 cgatctcacc ccgaccttct gcgccgccca caccaacgcc aacccgccga gggccatggc
       61 gagaaaggcc acgttgagct cgtcggtgta gaagatggcg atgaccgcga tcgccccgat
      121 gtcgtcggcg atcgccaggg cgaggaggaa caatttggcc ccgcccggca ccgtgctccc
      181 gagcagtgcc acctctccga cggcaaaagc gatgtcggtg gccatcggga ttccccagcc
      241 gcgaaccgcc tccccaccgg tgccggcggt gagggcgatg tagatgaggg cgggaacgat
      301 catcccaccc agtgctgcca tcaccgggag ggcggcagct cggcggtctc tcaggtcccc
      361 gaggatcagc tctcgcttga tctcgagacc gacaacgaag aagaagaccg ccatcaggcc
      421 gtcgttgatc agatgatgca ggctctcgtc gaagtcaaag gggccgaact ggacgctgag
      481 gtgggtctcg aggacctgga agtaggtgtc ggagaaggcc gagttggccc agaccaacgc
      541 cgcgatcgcg gctgcgagca agacgatccc cgatgacgcc tcgatttggg tgaagcggac
      601 aaaggggcgg acgaatcgtc tcggaaccag gcgctccgat gtggcccagg tctgatgcct
      661 gcgatccatc aaccgatggt tttaccggct gacagcagct gctccgcggc ctcgaccacc
      721 cgagccgaca tcccgttctc tgccttcttc aagtaggagc gggggtcgta tgccttcttg
      781 tcgccgacct ccccgtccac cttcagaacc ccgtcatagc tcttcatcac gtggtcgacg
      841 atcgggcggg tatacgcgta ctggcagtcg gtgtcgacgt tcatcttgac cactccgtag
      901 gccagcgtct cgtggatctc ctcgatcgag gacccgctgc ccccgtggaa gacgagatag
      961 tgacgggcct cgtcgccgta acgctccgcc accgccgcct ggccatcgcg gaggatcgtc
     1021 ggttcgagtc tgaccgcccc cggcttgtag acgccgtgca cattgccgaa gaccgcggcc
     1081 agaaggtagc gaccccggtc aaacaccccg ggcacctcgg acacccggac catgtcctcg
     1141 ggtgaggtgt agagcttggc ccgatcgacg tcctcgttgt cggtggcatc ttccaccccc
     1201 ccgacgatcc ctatctccag ctcgaggacg atgtcggcct cggcgcacaa cccgaggagc
     1261 tcgctggcca gggcaaggtt ctcctccatg gggaggttgg aggcgtcgag catgtgcgac
     1321 tggaacaacg gtcccttgcc ctccgtcttc cgcttcaggc tctcttcgat cagcggtctc
     1381 aggtaaccgt cgacccggtc cggcgggcaa tggtcggtat ggagagccac gttcacctgg
     1441 tactgctcgg cgaccactcc ggcgaactcg ctcagtgcct tggcgccaat cggaatgttc
     1501 ttgttggact ggccggacgc gaactccgcg cctccatagg agacctgaag gatgccatcc
     1561 gatgctgcct ggctcagacc ttccatggct gcgatcaccg tgctcgacga ggtgacgttg
     1621 atggcgggga gggcaaagcc tccattcctg gcgccgtcca gcatctccag gtacttctgc
     1681 ggcgtggcaa taggcacgct tttctccttg tcggttctga tgtcccaagg tagtcagttc
     1741 tcgattgccg cagta
//
LOCUS       DATITW010000542         1774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_186888, whole genome shotgun sequence.
ACCESSION   DATITW010000542 DATITW010000000
VERSION     DATITW010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186888"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VLS86_07520"
     CDS             <1..325
                     /locus_tag="VLS86_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HSO50382.1"
                     /translation="QKRGELFIGVGEQVYEGMIIGEASRPSEMVVNAARPKQLTNIRT
                     HASDEAINLKPPREMTLETAIEWIADDELVEVTPNAIRVRKRYLTEAERKRFGKKIAE
                     PVAVP"
     gene            complement(365..556)
                     /locus_tag="VLS86_07525"
     CDS             complement(365..556)
                     /locus_tag="VLS86_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50383.1"
                     /translation="MFLTPCDRLGREVLIWASDLHGIANTEAGIVLRFRCACGDVAEL
                     LTGATAPVRISAHLADLAL"
     gene            793..1338
                     /locus_tag="VLS86_07530"
     CDS             793..1338
                     /locus_tag="VLS86_07530"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HSO50384.1"
                     /translation="MNFSHYGDGPVELAVDLVNTLETSGDEIGDLGGLGSFLSRHQDL
                     DTSSLPPARDSDLDRIRALRSKLREVFEAPDEPTAAALLNDILAEHGAVARLSVHSGE
                     PHLHFEPVGESVTSWLGAVSAMGLAGVVVDHGIERFGVCHASNCRDVFVDTTRNRSRL
                     HCCGTCSTREAVAAYRKRQSE"
     gene            1411..1719
                     /locus_tag="VLS86_07535"
     CDS             1411..1719
                     /locus_tag="VLS86_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50385.1"
                     /translation="MTAPSATRAVPVLWRIHDIVVGFLSGFGVGTVAGLFVNRLFEDN
                     VVVLISAAIGAVAGIYVLIQNHRDSPRFLNAVVIVTWVLLVLSAAFLTLLVVAIANFE
                     "
BASE COUNT          309 a          564 c          565 g          336 t
ORIGIN      
        1 gcagaagcga ggcgagctct tcatcggcgt cggggagcag gtctacgagg ggatgatcat
       61 cggcgaggcc tcgcgaccga gtgagatggt ggtcaacgcg gctcggccca agcagctgac
      121 gaacatccgg acccatgcct ccgacgaggc gatcaatctc aagccgcctc gagagatgac
      181 actggagacg gccatcgagt ggatcgccga cgacgagctc gtggaggtga cccccaacgc
      241 catccgggtg cgaaagcgct atctcaccga ggccgagcga aagcgcttcg ggaagaagat
      301 cgctgagccc gtcgccgtcc cctgagctgt cgttgaccga cctgccatcg gggacggcgg
      361 atgctcagag cgccaggtcg gcgaggtggg cactgattcg caccggagcg gtggccccgg
      421 tcaacagctc cgcgacatcc ccacacgcac accgaaatcg aagcacgatc ccggcttcgg
      481 tgttggcgat gccgtgcagg tcagaggccc agatcagtac ctcacggccg agtctgtcac
      541 acggggtcag aaacatctcg ctgctccttg ctcaccgtca tccgatagct cccgagcctg
      601 acggaacgtg ccctctggcc tctgcgggct tgctgacggc tctgcgccgc attgcgcctc
      661 gcctccaggg tgtggagggc tgtcctgtat gcgagtgctg ggtgtccgaa catcggagga
      721 cctcctcttc tggacgtaat aaccgtcctg ctgtacactc attaccgtac ccaaggagct
      781 gtaacaagtc aaatgaattt ctctcattac ggagacggcc ccgtcgagct ggcggtggat
      841 ctggtgaaca ccctggagac cagtggcgac gagatcggcg atctgggggg actgggcagt
      901 tttctcagtc gtcaccagga cctggacacc agttcactgc ccccggcccg cgattcggac
      961 ctcgaccgga ttcgcgccct tcgctcgaag ttgcgtgagg tgttcgaggc gcccgacgag
     1021 cccactgcag ccgccctgct caacgacatc ctcgccgagc atggcgcggt cgcccgtctc
     1081 tccgtccaca gcggcgagcc ccatctccat ttcgagccgg taggggaatc cgtgacttcg
     1141 tggctgggcg ccgtctccgc catgggtctg gccggggtgg tggtcgatca tgggatcgag
     1201 cgattcgggg tgtgccacgc cagcaactgc agagacgtgt tcgtcgacac cacccgcaac
     1261 cgatcgcggc tgcactgttg cgggacctgt tccacgaggg aggcggtagc cgcctatcgc
     1321 aagcgacagt ccgagtgaga ctcgttggtt ggtagattct ccggcccggc ttggatcatt
     1381 gtcaccggat cgaggagcga aggaagcaca ttgaccgcac catccgccac aagggccgtt
     1441 cccgttctgt ggcgaattca cgacatcgtg gtcgggtttc tctcaggctt cggcgtcgga
     1501 accgtcgccg gtctcttcgt caaccgcctc ttcgaggaca acgtggtggt cctgatctcc
     1561 gccgccatcg gtgcggtggc cgggatctac gtcctgatcc agaaccaccg ggattcgcca
     1621 cgattcctca atgccgtcgt gatcgtcacc tgggtgctct tggtcctctc ggcggcgttc
     1681 ctgacgctgt tggtggtggc gatcgccaac ttcgagtagt tgacagtgct cagtgatcgg
     1741 tgggcccggc cagggcgtag gccacggcct gatc
//
LOCUS       DATITW010000543         1831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_387393, whole genome shotgun sequence.
ACCESSION   DATITW010000543 DATITW010000000
VERSION     DATITW010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387393"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VLS86_07540"
     CDS             <1..266
                     /locus_tag="VLS86_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50386.1"
                     /translation="RGSVEALARSVAAVEGGGTTVIGLGIGDHTVDLAYRRHEVVEQP
                     EGLAQAMIDGTRSALRRSLALWGMDTWWLRASDHQTRQERSIA"
     gene            259..1248
                     /locus_tag="VLS86_07545"
     CDS             259..1248
                     /locus_tag="VLS86_07545"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSO50387.1"
                     /translation="MPETATFHDPSGEGPSIELARFDVPKGLEDRDLRIRKIPEPDPY
                     YVDLGMLYRFAQIEQARRSGAPGLVPLHVAVRGHMGTGKDHDIEQFAAAMGLPYFRIP
                     LTGEVRDVTLIGSTRLHGDGKGGTESRWEDGDITRALRGPSLLNLSELNAAGAETLFA
                     LHGLLDRYAALDLPHGETVALRDDVRIFGTMNPTDLRDYAGTQTLNKAFADRWVIWEK
                     SFPGSEQVEAMLTRRYPELKTDYVEAISRLAVEVNASFEAQDSGIRVETPLSLRSILD
                     RIPTGLIVHRAGSDPLRSTWEEYVLPHVDQFDRDHYETVWNAVVRKGPSVSPF"
     gene            1289..>1831
                     /locus_tag="VLS86_07550"
     CDS             1289..>1831
                     /locus_tag="VLS86_07550"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HSO50388.1"
                     /translation="MAYDLGKPELDEKVRELVAATSSHENQELIAELVTTVLKLHRDG
                     ASRADLRLMNTALKEMRYSNLVFSRHREPKVTIYGSARITEQDPNYRIAEDFAARIVT
                     KGWGVITGAGPGIMEAGTRGAGIENSYGVNIRLPFEAAANRFLEEARTVNFKYVFTRK
                     LAFVKESHAFAILPGGWGTLD"
BASE COUNT          350 a          614 c          574 g          293 t
ORIGIN      
        1 cccgcggctc ggtcgaagcc ctcgctcgat cggtcgcggc cgtcgagggc ggaggaacca
       61 ccgtcatcgg cctcggcatc ggggaccaca cggtcgatct cgcctatcgc cgtcacgaag
      121 tggtggaaca gcccgagggc cttgcccaag ccatgatcga cggcacccgg agtgcattgc
      181 gtcgcagcct cgccctatgg ggaatggaca cctggtggct gcgcgcttcc gaccatcaaa
      241 ccagacagga gagatccatt gcctgaaact gccaccttcc atgatccaag cggcgaaggg
      301 ccgagcatcg aattggcccg gttcgatgtg cccaaaggtc tcgaggaccg ggacctgcgg
      361 attcgcaaga tcccggagcc ggatccctat tacgtcgacc tggggatgct ctatcgattc
      421 gcccagatcg aacaggcgag gcgctcgggc gctcccggac tggtgccgct ccacgtcgcg
      481 gttcgcggcc acatgggaac cggcaaggac cacgacatcg agcagttcgc cgcggccatg
      541 gggctcccct acttccggat cccgctcacc ggagaagtcc gcgacgtgac cctgatcgga
      601 tcgacccgcc tccacggcga tggcaagggg ggtaccgaga gccggtggga ggacggggac
      661 atcacccgcg ccctgcgcgg cccctccctc ttgaacctct ccgagctgaa cgccgcagga
      721 gccgagaccc tgttcgccct gcacgggctc ctggatcgat atgcagcact cgacctcccc
      781 catggcgaga cagtcgccct ccgcgacgac gtccgcatct tcggcacgat gaacccgacc
      841 gacctgcgcg actatgccgg cacccagacg ctgaacaagg ccttcgccga ccgctgggtg
      901 atctgggaga agagctttcc cggaagcgag caggtcgagg cgatgctcac ccgccgctat
      961 cccgagttga agaccgatta cgtggaggcg atctccagac tggcagttga ggtgaacgcc
     1021 tccttcgagg cccaggacag cgggatccgg gtcgagacac cgctttccct gcgctccatc
     1081 ctcgaccgaa tccccaccgg tctcatcgtg caccgggcag gcagcgatcc gctccgaagc
     1141 acctgggagg agtatgtgct tccgcatgtc gatcaattcg accgggacca ctacgagacg
     1201 gtgtggaatg ccgtggtgag aaaaggcccc tcggtcagcc ctttctagag gcctccgcat
     1261 caccggcggg gccgtataac gtcgacccat ggcctacgac ttggggaagc ccgaactcga
     1321 cgagaaggtg cgggagctgg tcgcggcgac ctcgagtcac gagaaccagg agctgatcgc
     1381 cgaactggtc accacggttc tcaaactgca tcgggacggt gcgagccggg ccgacctccg
     1441 tctgatgaac accgccttga aagagatgcg gtattcgaat ctcgtgttct cccgtcaccg
     1501 cgagcccaag gtcaccatct atgggtcggc ccggatcacg gagcaggatc ccaactaccg
     1561 gattgccgag gatttcgccg ccaggatcgt gaccaagggc tggggcgtga tcaccggggc
     1621 cggaccgggg atcatggagg cgggcacccg cggcgccggc atcgagaact cgtacggggt
     1681 gaacatccga ctcccgttcg aagcggcggc caaccgcttt ctggaggagg cgcgcaccgt
     1741 caatttcaag tacgtcttca cccgaaaact ggccttcgtc aaggaatcgc acgccttcgc
     1801 cattctcccc ggcggatggg ggaccctgga c
//
LOCUS       DATITW010000544         1521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_588200, whole genome shotgun sequence.
ACCESSION   DATITW010000544 DATITW010000000
VERSION     DATITW010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588200"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VLS86_07555"
     CDS             complement(<1..861)
                     /locus_tag="VLS86_07555"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSO50389.1"
                     /translation="MNDRVTIFDTTLRDGEQAPGIALTPDEKVTIAHQLAKLRVDVLE
                     AGFAASSPGDFEAVSRIAAGVRGPVIASLARAHPDDIDKAHDALKTADRYRIHVFMST
                     SAIHMEKMLRLTPDEVMKASIEGVRRARGYTDDVEFSPQDATRSDPDFMIAVCKAAVE
                     AGATTINIPDTVGFATPIEFAELLKRVFKEVRGDREDVIMSVHCHNDLGLAVANSLAS
                     LAAGARQIEGAINGIGERAGNTSIEEVVMAIKTRQDVYGVDLGVDTTQLYDTSRLVAR
                     LTGYPVQYNKA"
     gene            complement(1005..>1521)
                     /locus_tag="VLS86_07560"
     CDS             complement(1005..>1521)
                     /locus_tag="VLS86_07560"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843248.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HSO50390.1"
                     /translation="LGLSYAWAIGGTRAGVLLTTFKEETETDLFGEQVILCGGLSQLV
                     KNAYETLVDAGYQPESAYFETLHELKLIVDLRYEGGLSWMRYSISDTAEYGDYTRGSR
                     IVNEESKAEMRKVLDEIQSGAFANEWLAQAREGAPFLLEQRAANRNHPIEVVGAELRE
                     LMPFMKPKHAP"
BASE COUNT          264 a          465 c          491 g          301 t
ORIGIN      
        1 agccttgttg tactggaccg gatagccggt caggcgcgcc accaggcggg acgtgtcata
       61 gagctgggtg gtgtcgaccc cgaggtcgac cccgtatacg tcctggcggg tcttgatcgc
      121 catgaccacc tcttcgatcg aggtattgcc ggcccgctca ccgatgccgt tgatggcgcc
      181 ttcgatttga cgggctccag ccgccagaga cgcaagtgag ttggccaccg ccaggccgag
      241 gtcgttgtga cagtgcacgg acatgatcac gtcctcgcgg tccccccgca cctctttgaa
      301 aacccgcttg agaagttcgg cgaattcaat cggggtggcg aacccaacgg tgtcggggat
      361 gttgatagtg gtcgctcccg cctccaccgc cgccttgcaa acggcgatca tgaagtcagg
      421 atcggatcga gtggcgtcct ggggcgagaa ctcgacgtcg tcggtgtagc cgcgggcccg
      481 tcgcacccct tcgatcgatg ccttcatcac ctcatcggga gtgagccgca gcatcttctc
      541 catatggatc gccgacgtcg acatgaagac atggattcga tagcgatccg ccgtcttcag
      601 cgcgtcgtgg gccttgtcga tgtcgtcggg atgggcgcgg gccaacgagg cgattaccgg
      661 gccccggaca ccggcggcaa tacgcgaaac agcttcgaag tcgccggggg aggaggcggc
      721 gaatccggcc tccaggacgt ctacccgcag cttggccagc tgatgggcga tcgtgacttt
      781 ctcatcgggg gtcagggcga tccccggggc ctgttccccg tcccgaagag tggtatcgaa
      841 aatagttacg cggtcgttca tgtcgctgcg ctccagtcgg aagtcggaag tcggaaggcg
      901 gaagtcagaa ggccgagtcg gcttatcgcc gctcggccat caaagcgcca gcagcccgcc
      961 agcagacagg gtcacttccg tcttccgtct tccgtctgct ttgctcatgg cgcgtgcttg
     1021 ggcttcatga agggcatcag ctcgcgaagc tcggctccca ctacttcgat cgggtggttg
     1081 cggttggctg ctcgctgctc gagcaggaag ggtgctccct cacgcgcctg agccagccac
     1141 tcgttggcaa aagcacccga ctggatctcg tcgaggacct tgcgcatctc ggccttggac
     1201 tcctcgttca cgatccgcga gccgcgggtg tagtcgccgt actcggcggt atccgagatc
     1261 gaatagcgca tccacgacag gccgccctcg tacctgaggt cgacgatcaa cttgagctcg
     1321 tggagggtct cgaagtatgc ggactcgggc tgatagccgg cgtcgaccag ggtttcgtag
     1381 gcgttcttga ctagttggga gaggccgccg cagaggatta cctgctcgcc gaacaggtcg
     1441 gtttcggttt cctctttgaa agtcgtgagc aggactccgg cccgggttcc cccaatagcc
     1501 caggcatagg agaggccgag g
//
LOCUS       DATITW010000545         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_708312, whole genome shotgun sequence.
ACCESSION   DATITW010000545 DATITW010000000
VERSION     DATITW010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708312"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VLS86_07565"
     CDS             <1..530
                     /locus_tag="VLS86_07565"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HSO50391.1"
                     /translation="PGDLAAVFEPEPYEEIGPVVSSSIRDLANLTTIEVVEYTIVEKG
                     TDAGWLAWARGDSLQLFAVAKIGAGVDLNSVSVRNVSLGEDGVVEMTVPAAEVQYVAV
                     DNEATHILDRSTGVFTKGDPQLETEARRVAEEVLVAGALEDGLLAEAEANAREVLTDF
                     LLGLGYRDVVVEFEG"
     gene            660..1025
                     /locus_tag="VLS86_07570"
     CDS             660..1025
                     /locus_tag="VLS86_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSO50392.1"
                     /translation="MFDFPVDRQQAVLFLGREGHHILIAYVDGAPVGFVSGVETTHPD
                     KGTEMFLYELSVDEGHRRRGIGKALASALAELARERGCYGMWVGTEPENLGAIATYRS
                     AGAEDVEPFVTMSWTFPVS"
     gene            1226..>1709
                     /locus_tag="VLS86_07575"
     CDS             1226..>1709
                     /locus_tag="VLS86_07575"
                     /inference="COORDINATES: protein motif:HMM:NF025757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HSO50393.1"
                     /translation="MTTHKKLKQHIRARMEKTGESYMTARRHVVGSAGDQPGIGIHPD
                     TSALARILADSGVRGPDGPLTEAFVLGVAGGLGAGYILWEFSPETDHRPSRHLGADRR
                     VVVAGFRNSWQYPDRWADTVLERLGVSFRREQTGGSAKASRQLDEALESGRSAMVDIS
                     V"
BASE COUNT          291 a          534 c          594 g          290 t
ORIGIN      
        1 cgcccggaga cctcgccgct gtcttcgagc ccgagcccta tgaggagata ggcccggtcg
       61 tgagcagctc gatccgagat ctggccaacc tgaccaccat cgaggtggtg gagtacacca
      121 tcgtcgagaa ggggaccgac gccggctggc tggcctgggc gcggggagac agtctccagc
      181 tgttcgccgt ggccaagatc ggcgccgggg tggatctcaa ctcggtatcg gtgcgaaacg
      241 tctcactggg agaagacggg gtggtggaga tgaccgtccc cgccgccgaa gtccagtacg
      301 tcgccgtgga caacgaggcg actcacatcc tcgaccggag caccggtgtg ttcaccaaag
      361 gtgatccgca gttggagacc gaggcgcgac gggtagccga ggaggttctc gtcgccggag
      421 ccctggagga tgggctgctg gcggaggcgg aagccaacgc cagggaggtg ctcaccgact
      481 tcctgttggg cctgggttat cgcgacgtgg tggtcgagtt cgagggctag gtgtcctccg
      541 gtcgacgacc atgatggagg ccggatcgct gtctcgcctg gccggtgagt gagtcgaccg
      601 acatcgagat tcggcgtctc cgagccgagg atgtcgacgc cgtggtcgaa gcctcgtcgt
      661 tgttcgactt cccggtcgac agacaacaag cggttctctt cctcggcagg gaaggccatc
      721 acatcctcat cgcatatgtc gatggggctc ctgtcggatt cgtgagcggc gtcgaaacga
      781 cccaccccga caagggaacc gagatgttcc tctacgagct cagcgtcgat gaaggccacc
      841 gccggcgtgg gatcggcaag gcactggcat ctgccctggc cgagctggcc cgagagcggg
      901 gctgttatgg gatgtgggtc ggcaccgaac ccgagaacct gggggccatc gccacctacc
      961 gatcggctgg ggccgaggat gtcgagccgt tcgtgactat gtcgtggact tttcccgtct
     1021 cctgacacca ccctgcaatc agtctcgcta gcctctcggt ccggcgtgct cctgcgccgc
     1081 atctgctgac cgggcgccca cgccgcccgg cgaagcgcag ggaaaggtgg cccatgccat
     1141 cgggagtgct ctcgaacgtc gtcgacctga tggcgaccgc caggcgaggt gtgggctcgt
     1201 ggcggccatg ccatggagat cgatgatgac cacacacaag aagctcaagc agcacatccg
     1261 cgcccggatg gagaagaccg gcgagagcta tatgaccgcc cggcgtcacg tggtcggctc
     1321 tgcaggcgac caacccggca tcggcatcca ccccgacacc tccgccctgg cgaggatcct
     1381 cgccgactca ggtgttcgcg ggcccgacgg cccgctcacc gaggcgttcg tgctcggggt
     1441 tgcgggcggg ctcggagccg gatacatcct gtgggagttc tcacctgaga ccgatcacag
     1501 accctcccgt catctcggtg ccgaccgccg cgtcgttgtc gccgggtttc ggaattcttg
     1561 gcagtacccg gatcgttggg cggacacagt cctggagcgg ctcggggtgt cgttccggag
     1621 ggagcagacc ggaggctcgg cgaaggcgag ccggcagctc gacgaggccc tggaatcggg
     1681 gcggtcggcc atggtcgaca tatcggtcg
//
LOCUS       DATITW010000546         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_27522, whole genome shotgun sequence.
ACCESSION   DATITW010000546 DATITW010000000
VERSION     DATITW010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27522"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VLS86_07580"
     CDS             <1..675
                     /locus_tag="VLS86_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSO50394.1"
                     /translation="IVDLGCHCIEIIRSFMGKENRPVEVMAWADTLVHPIEAEDNAVA
                     LIRFESGALGQFEVSWTFRGGMDLRDEVAGTEGTIWLNHFLRTGFEMYSSGGGNGYIA
                     EKLESTEGWLFPVGDEISELGYTAMFADMFDSMDRGGSPRETLYDGYVVNAVMDAAYR
                     SAESRQWEPVEMAWRGAGTPRITKSVATHEGHAIVKEEILPDGRRKLILSDKDTGSVF
                     DLVTPG"
     gene            672..1349
                     /locus_tag="VLS86_07585"
     CDS             672..1349
                     /locus_tag="VLS86_07585"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HSO50395.1"
                     /translation="MSLEVEILPDEDWPTLVGRRLTETIAHSPQARICLATGNTIAPV
                     YRHTEVSGDPSIFLLDEYGGLPRDDPARCAAMFRRDFAHPRFLAPDVDAADPHAAADR
                     YGETVRERPLDLAVVGLGRNGHVGMNEPGSTMGSITRKVELSETTSLGALEYGASMRP
                     VWGITVGIAELMSAMQLWLLVTGAHKAEILAEAMRCPIGPELPASYLRDHPNARLLAD
                     TAAARLL"
     gene            complement(1346..2287)
                     /locus_tag="VLS86_07590"
     CDS             complement(1346..2287)
                     /locus_tag="VLS86_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50396.1"
                     /translation="MPFARTVLGDVDPARLGVVLAHEHLIIDSPIVESRWPHIHLPST
                     AEAIAEVGHCLGVGIATMVDAMPVGSGGDPVRLAEISTATGSHVIASTGLHTAKYLEG
                     VEWALTGSVDDLSLRFTESIERGVDGARTGVLKVATAGQVPDAFEHRLFEAAAATHLA
                     TGVPILTHCEEGMGGMPQIELLASLGISPERLALSHTDRVLDSAYHRDLMETGANLCF
                     DQGLRSPEKTARLVAELTALGFGSQLLVGTDGARRSLWSTLGGSPGLAWIHAGFREVL
                     AAHGVDDETLGRIFVGNPARWLTFSPGSGSSHMPLGH"
     gene            complement(2287..>2582)
                     /locus_tag="VLS86_07595"
     CDS             complement(2287..>2582)
                     /locus_tag="VLS86_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSO50397.1"
                     /translation="PGTGTTWEYLPDGAVVGIPLSSLVVRDGQNILASGRCFSATHDA
                     HASIRSMAQCMAMGQAAGTTAAMAAAGSGEIRSVDPAAVQAALRSAGAVLELP"
BASE COUNT          466 a          848 c          873 g          395 t
ORIGIN      
        1 atcgtggacc tcgggtgtca ctgcatcgaa atcatccgaa gcttcatggg aaaggagaac
       61 cggccggtcg aggtgatggc ctgggccgac actctggttc atccgatcga agccgaggac
      121 aacgcagtcg cgctcattcg attcgagtcg ggcgccctcg gccagttcga ggtgagctgg
      181 acgttccggg gcgggatgga tctccgtgac gaggtggcag ggaccgaagg caccatctgg
      241 ctcaaccatt tcctgcgaac cggcttcgag atgtacagct ccggtggggg aaacgggtac
      301 atcgccgaga aactcgagtc gaccgagggc tggctgttcc cggtgggaga cgagatcagt
      361 gagctcggat acacggccat gttcgccgac atgttcgact cgatggacag aggagggtcg
      421 ccacgcgaga ccctctacga cggttacgtg gtcaacgcag tgatggacgc cgcgtatcgc
      481 tccgccgagt cgcggcaatg ggaaccggtc gagatggcct ggcgcggcgc gggcacaccg
      541 cgcatcacca agagcgtcgc cacccacgag ggccatgcca tcgtcaaaga ggagatcctc
      601 cccgacggcc ggcgcaagct gatcctcagt gacaaggaca ccggatcggt gttcgatctc
      661 gtcactccgg ggtgagcctc gaagtcgaga tcctccccga cgaggactgg cccacgctgg
      721 tcggcagacg gctgaccgag acaatcgccc acagcccgca ggcgcggatc tgcctggcca
      781 ccgggaacac catcgccccg gtctaccgac ataccgaggt ttcgggtgat ccgtcgattt
      841 tcctcctcga cgagtatggc ggtctccccc gagacgaccc ggcgagatgc gccgcgatgt
      901 tccgccgcga tttcgcccat cctcgtttcc tggcacccga cgtcgacgcg gcggacccac
      961 atgccgcagc cgaccgatat ggagagaccg tgcgcgagcg accactcgac ctggcggtgg
     1021 tcggcctggg acgcaacggg cacgtcggga tgaacgagcc cggctcgacg atgggctcca
     1081 tcaccaggaa ggtggagctg tccgagacca ccagtctcgg ggccctggag tacggggcgt
     1141 cgatgcgccc ggtctggggg atcaccgtgg gcatcgccga gctcatgagc gcgatgcagc
     1201 tctggctctt ggtgaccggc gcccacaagg cggagatcct ggcggaagcc atgcgatgcc
     1261 cgatcggccc cgagctaccc gccagctatc tccgcgacca tcccaacgcc cggctgctcg
     1321 ccgacaccgc tgctgctcgc ctcctctagt ggcccaacgg catatgagag ctgccgctcc
     1381 ctggcgagaa cgtcagccag cgggcgggat tgcccacgaa gatccgaccc agtgtctcgt
     1441 cgtccacgcc atgagcagca aggacttccc ggaacccggc atggatccac gccaaaccgg
     1501 gcgacccgcc aagggtcgac cacagcgagc gtctcgcgcc gtcggtgccg acgaggagct
     1561 gtgacccgaa cccgagagcg gtcagctcgg cgacgaggcg tgctgtcttt tcggggctgc
     1621 ggagaccctg atcgaagcag agattggccc ctgtctccat cagatcacgg tgataggcgg
     1681 agtcgaggac ccggtcggtg tgggacagcg ccaaccgctc gggtgagatc cccaaggacg
     1741 cgagaagctc gatctggggc atgcccccca tgccctcctc gcagtgggtg agaatcggga
     1801 ctccggtggc gagatgggtc gcggcggcag cctcgaacag ccgatgctcg aacgcatcgg
     1861 ggacctggcc cgcggtggcg accttgagca ctccggtccg ggcgccatcg acacctcgtt
     1921 ctatcgactc ggtgaagcgc agggacaggt cgtcgacgga cccggtgagt gcccactcga
     1981 ccccttcgag gtatttggcg gtgtgcagcc cggtggaggc aatgacatgg gacccagtag
     2041 cggtggagat ctcggcgaga cgaaccgggt cacctccgga gccgacgggc atcgcatcca
     2101 ccatcgtggc tatgcccacc ccgaggcaat ggcccacttc ggcgattgcc tcggccgtgg
     2161 agggcaggtg tatgtgcggc cagcggctct caacgatcgg gctgtcgatg atgagatgct
     2221 cgtgggcgag gaccactccc agcctcgcgg ggtcgacgtc accgaggacg gtgcgcgcga
     2281 acggcatcag gggagctcga gcacggcgcc ggccgatctc agcgcagcct gcaccgcggc
     2341 gggatcaacg gaccgaatct cgcctgatcc ggcggcggcc atggctgcgg tggtcccggc
     2401 ggcctgcccc atggccatgc actgggccat cgacctgatc gaggcgtggg catcatgggt
     2461 cgccgagaag cagcggccag aggccaggat gttctgcccg tcacgaacca cgaggctgga
     2521 cagcgggatt cccaccaccg cgccatccgg caggtactcc caggtggtgc cggtccccgg
     2581 ct
//
LOCUS       DATITW010000547         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_227599, whole genome shotgun sequence.
ACCESSION   DATITW010000547 DATITW010000000
VERSION     DATITW010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227599"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..521
                     /locus_tag="VLS86_07600"
     CDS             372..521
                     /locus_tag="VLS86_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50398.1"
                     /translation="MTEVDEQRGDGEIGKCHVCGSEFSTQEELSKHLMDVHEDDLLPE
                     AQDEA"
     gene            complement(518..1472)
                     /locus_tag="VLS86_07605"
                     /pseudo
     CDS             complement(518..1472)
                     /locus_tag="VLS86_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(1778..2047)
                     /locus_tag="VLS86_07610"
     CDS             complement(1778..2047)
                     /locus_tag="VLS86_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HSO50399.1"
                     /translation="MIAAIFAQPDFSGLKIDIGESAITYGNFLNALITFLIVAFVMFL
                     IVKAYNRVTQPKAATTKSCPFCQTDIAIGATRCPNCTSQLEGAPA"
     gene            complement(2005..2178)
                     /locus_tag="VLS86_07615"
     CDS             complement(2005..2178)
                     /locus_tag="VLS86_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HSO50400.1"
                     /translation="MANVLKEFRDFITKGNLIEIAVAFIMALAFAAVVASFVADIVTP
                     SSRRSSRSRTSAG"
BASE COUNT          421 a          784 c          808 g          435 t
ORIGIN      
        1 cttgttcttc acgaataccc acagttgtct cagtcaaaac ggataggccc tttcagcgca
       61 gctcggatcg gaaactacag ctagggcttg ctcctcaggc ccccggagcc gccttctgcc
      121 attggctcag ctggggtccg cctcatccag gctgccacct aggccggcaa gagaggaggt
      181 ctcctacggc tgccaccacg aatgagaccg tggcgctcac tgggccgtct tcgagccaag
      241 gccgccaaga ggatggcaac ccgagcgcga ttcctgcaca ctgcgctagg cgagggtgac
      301 cttccaggta gggagggcct tgctatgcgc aacgcgagga atcggattgg attcaatgaa
      361 ggaggtcgtg gatgaccgaa gtcgacgagc agcgcggcga cggcgagatc ggcaagtgcc
      421 atgtatgcgg gagtgagttc tcaacgcagg aagaactctc gaagcacctg atggatgtgc
      481 acgaagatga cctgctcccg gaggcacaag acgaggccta ggtgtgcttc ctcgggacgt
      541 tgttcacgac cgtgatcggc ggcctgcctg catgcgccat gtgaggacga tggtagttgt
      601 agtcgtggag ccatcggtcg agcgcctcgg cccgtgagtc gttcgtctca taggcctcgg
      661 taggcccact cccacttgag cgtcaggttg aaccgctcga ccttgccgtt tggtctgggg
      721 ccggtaagga cgagttcgtc ggtggcccac cccggcctta gccaggaccc gggcgaaggg
      781 tcgcgagcgg tagcacccgc cgttgtcggt gagcacgcgc tcgaccttca cccccaccgt
      841 ccaagaagaa gccgagcgct cgggtcatga acgcggaggc ggtctccttg cgctcatcgg
      901 cgaggatctc cgcgtaggcg aggcgcgagc gatcatcgac cgcggcgtgg atgaagtcgt
      961 agccctgacc ccggagtttc cgggagccgg cccgtcggtc cctgccgtgg atccgccacc
     1021 ctcctcccgc cgggatcctg ccctgcttct tcacgtcgac gtggacgagc tcgcccggtc
     1081 gctcgcgctc gtatcgaacg atggtccgag tgggccggtc gagatcccgc aagcgtggtc
     1141 actgagccga cggagcaccc ggtgcacggt cgaaggtgct tccctgagcg cccaggcgat
     1201 ccggtgcggg ccctcgagcg tggcgtggcg cctcgagggg atcgctgcct ctcggcgggt
     1261 gctgagccga gccgggctcc ggtgcggcct actcgaacga tcccggaggc cacccggtcc
     1321 ctcggccgtg aaccggcgga tccacttgta gccgtcgccg aggagcaccc ctgcgcctca
     1381 gcggccatcg ggaccgtcca gcccagttcg agaacccgct ccaccaggag ctgacggcca
     1441 tggacggtga gcttcgccct gggatgggac atgagagcct cctttctgac ggattagtcc
     1501 ctgtcgagca gggaggctct cagttcttga accacgtgtt ggggaactac acctagggcg
     1561 cggaaggcaa ccagcacggg tcgccaggct acagggcggt cggccgcgtc gtggagtcac
     1621 tagcgcgcgg cctcccgcag catcccaacg attacggccc tgagcacccg gtcctcctcc
     1681 cagtcgatca ggagctcggc gaaggtgcag gtctcggggt tcctccagta cgttccgatc
     1741 gcgtcggtcc gcgcggatag aacgcaggct gttgacgcta ggccggagct ccttcgagct
     1801 ggctcgtgca gttcgggcac cgggtggccc cgatcgcgat gtccgtctga cagaagggac
     1861 agctcttcgt cgttgccgcc ttcggctggg tcacgcggtt gtacgccttc acgatcagga
     1921 acatgacgaa ggccacgatc aggaaggtga tcagcgcgtt caggaagttg ccgtacgtga
     1981 tcgcgctctc accgatgtcg atcttcagcc cgctgaagtc cggctgcgcg aagatcgccg
     2041 cgatgatggg gtcacgatgt cggcgacgaa cgaggcaacc accgccgcga acgccagtgc
     2101 catgatgaag gccacagcga tctcgatcag gttgcccttc gtgatgaagt ccctgaactc
     2161 cttcagcacg ttcgccactc cttcctgtgc gcgtctgacg gatgacgctc aatcttgcgt
     2221 ctcggcgtca gcctcgcgca acatgccgat gagcaccgcc cggagcgtcc ggtcttctcg
     2281 cagtcgatca gaagctcggc gaaggtgcgg ctctctggtt agccccagaa ctcgccgatc
     2341 cggtcggctc gctcgaagtc gggcagcatg aggacgtgga ggagttcggc gcggggagcg
     2401 ggagggaaag cgtcgaggcg ctggcgaaga gccctcctcc atctcctc
//
LOCUS       DATITW010000548         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_307670, whole genome shotgun sequence.
ACCESSION   DATITW010000548 DATITW010000000
VERSION     DATITW010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307670"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VLS86_07620"
     CDS             <1..270
                     /locus_tag="VLS86_07620"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HSO50401.1"
                     /translation="SVLLIAPSAHQRMRAPITGVERKSKRHLQMTINLTNAGTIVFAF
                     ALAAAVFLISTLVLSDSYAIAATVAVTALILWSWFYLPLVTFQRT"
     gene            complement(359..787)
                     /locus_tag="VLS86_07625"
     CDS             complement(359..787)
                     /locus_tag="VLS86_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HSO50402.1"
                     /translation="MALRSTGRTQWNGDLMSGSGTTTLDSGAAGPMQVTWKARTEEHG
                     GLTSPEELIAAAHASCYSMAFSSALAKAGTPPTTLETEATATFVPGEGITSMELTVRG
                     TVEGISDEDFLAAAENAKENCPVSKALKGNVPITLNASLA"
     gene            complement(855..1595)
                     /locus_tag="VLS86_07630"
     CDS             complement(855..1595)
                     /locus_tag="VLS86_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50403.1"
                     /translation="MTLEQRLSEALHEVDRFEPSPDLFARVERSLDEDATHRRRVRVT
                     WLAAMASLTAIVVFLGLLVRTGATGLLVFPVWGLELLETVILVSLIFVLAPVISRFGD
                     FYVADVFRLDPGTGKRFMRLLEIAYYLVFLGGVLQGVHLTRLGRVVRLGPGLETMLEG
                     IAELLLFMGVFHAMSLFGLPVVGLVFSSVVRRAERQRAGSSAPPISSKAQQADRIASW
                     VVQITFGLVLLGALVALIGGVVSIGFDS"
     gene            complement(1592..2143)
                     /locus_tag="VLS86_07635"
     CDS             complement(1592..2143)
                     /locus_tag="VLS86_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSO50404.1"
                     /translation="MYWAEALDASKESWTWAEIDRRELMETLYQREAVSLVRLARIFT
                     DDRNAAEDLVQEAFIRLFNSAHRIQDEARVSAYLRSIVLNLARDHNRRGLMSLRHQDV
                     MTDRWVPQQPDEEVVRTESQASVLAALRSLSPRQRDCLVLRFYLELSEGEIATTLDIS
                     PNSVKTHCRRGLAAMASRLEGAP"
     gene            complement(2147..2533)
                     /locus_tag="VLS86_07640"
     CDS             complement(2147..2533)
                     /locus_tag="VLS86_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50405.1"
                     /translation="MSWWAIELTTNLLLLAVVLVLGPLIKRFGRGYAGDVFRANPRTG
                     KSYLVLMDVAYYLIFVAYILFTVTLSKPLEWRQVGVQLEESLIRIGGMLLLMGVLHGL
                     NVLSLPLVGRLLGLSRQLEEDTPRTV"
BASE COUNT          502 a          875 c          800 g          445 t
ORIGIN      
        1 tcggtgctgc tcatcgcccc cagtgcccat cagcgaatgc gggccccgat caccggcgta
       61 gagcgaaagt cgaaacgaca tctccagatg acgatcaacc tgaccaacgc cgggaccatc
      121 gtcttcgcct ttgccctcgc cgcggcggtg ttcctgatct cgaccctggt gctcagtgac
      181 agctatgcga tagcggcaac cgtggccgtc accgcgctga tcctctggtc ctggttctat
      241 ctccccttgg tcactttcca gcgcacctga gtcgcgcttc ttcgtcaggt ctcaggcatg
      301 gggcgacctc tggccgcttc ttcctgcaca accttgcccg gtggctgcgg gcgcggcgtc
      361 aggccaggga ggcgttcagc gtgatcggga cgttgccctt cagcgccttg gagaccgggc
      421 agttctcctt cgcattctct gccgccgcca ggaagtcctc atcgctgatg ccttccaccg
      481 tgccccgtac cgtcagctcc atcgaggtga tcccctcccc tggcacgaag gtggcggtgg
      541 cctcggtctc cagggtggtt ggcggggttc cggctttggc cagggcgctg gagaacgcca
      601 tcgagtagca cgaggcatgg gcagcggcga tcagctcctc cgggctggtg agaccgccat
      661 gctcctcggt gcgagccttc cacgtcacct gcatcgggcc ggcggcgcca ctgtcgaggg
      721 tggtcgtgcc gctcccactc atcagatcgc cgttccactg ggtgcgaccg gtgcttctca
      781 atgccatttt ccaatcctcg ctcggggccg ggaacaatac cgattgtcgg gctcgggcat
      841 cccggtgcgc acgactagct gtcgaatccg atgctgacga cgcctccgat cagtgcgacg
      901 agagccccga gcagaaccaa gccgaatgtg atctgaacca cccaggatgc gatgcgatca
      961 gcctgctgcg cctttgacga gatcggcgga gcagagctgc ccgcgcgctg acgctcggcg
     1021 cgtcggacca cagaggagaa gacgagccca accactggca ggccgaagag ggacatggcg
     1081 tggaacacgc ccatgaagag aagcagctcg gcaatgccct ccagcatcgt ctccagcccg
     1141 gggccgagtc ggaccacccg ccccagccgg gtcaggtgga ccccctgcag cacaccgcca
     1201 aggaacacga ggtagtaggc gatctcgagc agccgcatga agcgcttgcc ggtgcccggg
     1261 tcgagacgaa acacgtcggc gacgtagaaa tccccaaaac ggctgatgac cggggcgagg
     1321 acgaagatca ggctcaccag gatcaccgtc tccaatagct caagtcccca gaccgggaac
     1381 acgagcagcc cggtagctcc agtcctgacc agcaaaccga ggaagacgac gatcgcggtg
     1441 agcgaagcca tggccgccag ccatgtgacc cgaacccggc gtcgatgggt cgcgtcctca
     1501 tcgagactgc gctcaacccg tgcgaacaga tccggactgg gctcaaaccg gtcgacctcg
     1561 tgcagggctt cggagagacg ttgctcgaga gtcacggggc gccctcgagc cgcgaggcca
     1621 ttgctgcgag cccacgacgg cagtgggtct tcaccgagtt cggtgagatg tcgagggttg
     1681 tggcgatctc accttcgctc aactccagat agaagcgcag gaccaagcag tcacgctggc
     1741 gtggtgacag cgaccgcagc gcagcgagaa cggaggcctg actctcggtg cgcaccacct
     1801 cttcgtccgg ctgctgcgga acccatcggt ccgtcatcac atcctgatgc ctcagtgaca
     1861 tgagaccgcg ccgattgtga tcccgcgcca ggttgaggac gatggaacgg agataggccg
     1921 acaccctcgc ttcgtcctgg atccggtgcg ccgaattgaa gagacggatg aatgcctcct
     1981 gcacgagatc ctcggcagcg ttccggtcgt cggtgaagat ccgggccagc ctgaccagag
     2041 acaccgcttc acgctgatac aacgtctcca tgagctcccg gcggtcgatc tccgcccagg
     2101 tccaggactc cttggaggca tcgagggcct cagcccaata cacaggtcag accgtgcgtg
     2161 gcgtgtcctc ttccaactgt cggctgagac cgagaagacg gccgaccagt gggaggctga
     2221 gcacattcag cccatggagc acgcccatca ggagcagcat gccaccgatg cggatcagcg
     2281 actcctcgag ttggacaccg acctgtcgcc attccaacgg cttgctcagc gtcaccgtga
     2341 acaggatgta tgcaacgaag atcaggtaat aggccacatc catcaacacg aggtagctct
     2401 tgccggtgcg cgggttggct cggaacacgt caccggcgta gccacgcccg aatcgcttga
     2461 tcaacgggcc gagcacgagc acgacggcga ggagcagcag attggttgtc agctcgatgg
     2521 cccaccagct caactgaatt cccttctcac atcgctgtca tcctggcact cccgccggct
     2581 cccggctcgc ctgccgggac ctgaacgtct cgacacggtc ca
//
LOCUS       DATITW010000549         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_788078, whole genome shotgun sequence.
ACCESSION   DATITW010000549 DATITW010000000
VERSION     DATITW010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788078"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VLS86_07645"
     CDS             complement(<1..1009)
                     /locus_tag="VLS86_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50406.1"
                     /translation="MSGTHPRDMLYTDPVPRIPLGSGERRYTDVHQAPQRDPDFDSQA
                     FHWGGLTAGSRLTAFWILLAPFALANVAGWMTADRDNRFGRSAIRLAGLGLTAMFLTQ
                     IFTAIVLLPYLWLAGRDEIGVFGAVIDVGTGLEKGVMLVLVLLISSLFLTLVLKASTQ
                     SHFSPLDTATQARLLIWPTLDSMLPAAPSEDLIPLPAEPDRFDDPAGTVITHPRLWRP
                     DSILNRLRRLHVGVGFGIMALGTSIWTDTPWSYLLSGILLAWIVVATFVTAFSPRSPL
                     VTWGTAVSPLAGALVLIISMVSIFTSDHAGWRPDSVHSVTFITTLLLGVFVFMSLAAG
                     WI"
     gene            1124..2131
                     /locus_tag="VLS86_07650"
     CDS             1124..2131
                     /locus_tag="VLS86_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007457689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HSO50407.1"
                     /translation="MEEERIALFVDFENLAIGVREALGGMEFDFEPIGNALAERGRVV
                     TRKAYADWSMFSDARQKLTRAHIELIEIPQRMGASRKNAADIKMAVDAIEVAFERDYV
                     TTFVICSGDSDFTPLVHKLRELNKRVIGVGVKASTSALLPPACDEFLFYENLDGVEPR
                     RPSEPTDTPEPEESATPELASLITTTLAGLQRSSGSEVIASSLKRALLRKDSTFSEAD
                     YGFRGFGELLNHLESQAVIELSEGPGRGDPVVGFAAEGSGEGAVFALLAEVVADLSKK
                     GNPVYLSGLKNQLRKKDGSFSEKKFGYAGFLQFCKAAATRGLIELTWDEEAGDYRVSV
                     A"
     gene            2128..>2370
                     /locus_tag="VLS86_07655"
     CDS             2128..>2370
                     /locus_tag="VLS86_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSO50408.1"
                     /translation="MSRTADVVVVGSGIVGSSIAYQLARHGVPGVVALDKGAGPAEGS
                     TGASSSICRCRYTHPQVVRLAYHGQEAYGNWPAFTGL"
BASE COUNT          466 a          695 c          823 g          386 t
ORIGIN      
        1 agatccatcc ggcggccaac gacatgaaca cgaagacacc tagaaggagg gtggtgatga
       61 aggtgaccga gtgcaccgag tccggacgcc aaccggcatg gtcgctggtg aagatgctga
      121 ccatcgagat gatcagaacc agggcgccgg ccagaggaga caccgccgtg ccccatgtga
      181 ccagagggct ccgaggactg aatgcggtga cgaaggtggc gacgacgatc catgccagca
      241 gaatgcccga gagcaggtag gaccacgggg tgtcggtcca gatcgacgtg cccagggcca
      301 tgatcccaaa tccgacgccc acgtggagcc gtcgcagccg attgagaatg gagtcaggtc
      361 gccacagtcg agggtgggtg atgaccgtcc ccgccgggtc gtcgaagcgg tccggctcgg
      421 ccggcaacgg gatcaggtct tcggatgggg cggcgggaag catcgagtcg agggttggcc
      481 agatgagcag ccgggcttgg gtggcggtgt cgagtggtga gaaatgggac tgggtcgacg
      541 ccttcaacac cagggtcaga aacaggctgc tgatgaggag caccaggacc agcatcacac
      601 ccttctccaa cccggtgccg acatcgatca cggcgccgaa caccccgatc tcatcccggc
      661 cggccagcca cagatagggg aggaggacga tggcagtgaa gatctgagtg aggaacatcg
      721 cggtcagccc gagaccggcg agccggatcg ccgagcgacc aaacctgttg tcccggtcgg
      781 cggtcatcca accggcgacg ttggccaggg cgaagggggc aagcaggatc cagaacgcgg
      841 tgagacggct tcccgcggta aggccacccc agtggaaagc ctggctgtcg aagtccgggt
      901 cacgctgggg agcctggtgg acatcggtgt agcgacgttc cccgctgccg agtgggatcc
      961 ggggcacggg atcggtgtac agcatgtctc gcgggtgggt gcccgacact ccgtgcaccc
     1021 tgagttcgct gaatctggcc agagtctccc ccttggtcga tgaggcgaga agctacgcga
     1081 gccggcacgg gagttcaccc acgccacgcc taccctttgc cctatggaag aagaacgtat
     1141 tgccctcttc gtcgacttcg agaacctggc catcggggtc cgagaggctt tgggcgggat
     1201 ggaattcgac ttcgagccga tcgggaacgc tctggcggaa cgaggccggg tcgtcaccag
     1261 gaaggcctac gccgactggt cgatgttctc cgacgcccga cagaagttga ccagggccca
     1321 catcgagctg atcgagatcc cccagcgcat gggcgccagc cgaaagaacg cagccgacat
     1381 caagatggcg gtggacgcga tcgaggtggc ctttgagcgt gactacgtga ccaccttcgt
     1441 catctgctcc ggcgattccg acttcacgcc tttggtgcac aagctgcggg agttgaacaa
     1501 gagagtcatc ggtgtcgggg tcaaggcgtc gacatcagcc ctgctgcccc cggcttgtga
     1561 cgagtttctg ttctacgaga atctcgacgg agtcgagcca agacgtccca gcgagccgac
     1621 cgataccccg gagcccgagg agtcggcgac tccggagttg gcctcgctga tcaccaccac
     1681 cctcgccggt ttgcagagat ccagtggcag tgaggtgatc gcgtccagtc tgaagcgagc
     1741 gctgctgagg aaggactcga ccttcagcga agcggactac ggcttcaggg gttttggcga
     1801 gctgttgaat catctcgagt ctcaggcggt catcgagttg tccgaagggc cggggcgtgg
     1861 tgacccggtg gtcgggttcg ccgcggaggg ctcgggggaa ggagcggtgt tcgccctcct
     1921 cgccgaggta gtggccgacc tgtcgaagaa gggaaacccg gtctacctct caggcctgaa
     1981 gaaccaactc cggaagaagg acggctcctt ctccgagaag aagttcggct acgccgggtt
     2041 cctccagttc tgcaaggcgg cggcaaccag aggtctgatc gagttgacct gggatgagga
     2101 agcgggcgac taccgggtca gcgtggcatg agtcgaaccg ccgacgtggt ggtggtgggc
     2161 tcggggatcg tcgggtcttc gatcgcatac cagttggcac gccacggtgt gccaggcgtg
     2221 gtcgccctgg acaagggcgc cggccccgcc gagggctcga ccggcgcctc atcgtccatc
     2281 tgccgctgtc ggtacaccca tccccaggta gtgcgtctgg cgtatcacgg tcaggaggca
     2341 tacggcaact ggccggcgtt cacggggttg
//
LOCUS       DATITW010000550         1543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_67822, whole genome shotgun sequence.
ACCESSION   DATITW010000550 DATITW010000000
VERSION     DATITW010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67822"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..771
                     /locus_tag="VLS86_07660"
     CDS             85..771
                     /locus_tag="VLS86_07660"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HSO50409.1"
                     /translation="MDQKMTTAVENWLKAGLPCDCAAILLAEVVGEPEAVEAEAALIS
                     RIGRANGARDVKVARDEAERAILWKGRKSAFGAVAQVSPNYYLHDTVVPRSRLVETME
                     EVQAIADRYGLDVYNVFHAGDGNLHPLMAFDASDPVMLDKVHKAGEEMVAACLSRGGA
                     LSGEHGIGLEKRDMMRADFTELDLDAQARLKEAFDPAGIFNPAKVLPEGSRCFDFGGV
                     RREIPDGAWV"
     gene            768..>1543
                     /locus_tag="VLS86_07665"
     CDS             768..>1543
                     /locus_tag="VLS86_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSO50410.1"
                     /translation="MTETAGGGPERVAPSTVEDLSAILKKATEEQRLVNVVGGGTRQG
                     YGSPPPPDLVLEMSGLSGIEEWDPDDLTVTVRAGTSVADLEAALGERSQTAVLPEIPG
                     TSTVGGVISAGISSLRRARLLGTRERMLEVRLVTGDGRVVRGGGRVVKNVSGSDLPRL
                     VIGAFGSLGVITSVCLKLWPIPPAAATITIDDIGVADRVTRPLAVLERNGLTEVFLWG
                     TPEEVAASGGRLGGAAIEGHRWPADPQGSYRWSVRVPPALT"
BASE COUNT          257 a          480 c          548 g          258 t
ORIGIN      
        1 ctcgacttcg aggagctgga aggggcgacg cggacggtct ctgaggtcat cgcatccggg
       61 ctggtgccgg cggcgctgta gatcatggac cagaagatga cgaccgccgt cgagaactgg
      121 ctcaaggcgg gcctgccctg cgattgcgcc gcaatcctcc tcgccgaggt ggtcggggag
      181 cccgaggcag tggaagccga ggcggctctc atctcacgca tcggccgcgc caacggggcg
      241 cgcgatgtca aggtggccag agacgaggcc gagcgcgcca tcctctggaa ggggcgcaag
      301 tcggcgtttg gggccgtggc ccaggtgtcc ccgaactact acctacacga cacggtcgtg
      361 ccgcggtcgc gtctcgtaga gaccatggaa gaggtccagg ccatcgccga ccgatacggg
      421 ctggacgtct acaacgtgtt ccatgccggc gatgggaatc tccatccgct catggcgttc
      481 gatgcctccg acccggtcat gttggacaag gtccacaagg cgggcgagga gatggttgcg
      541 gcatgtctca gccgaggtgg cgccctctct ggtgagcatg ggatcggcct cgagaagcgg
      601 gacatgatgc gggcggattt cacggagctc gacctcgacg cccaggcccg gctcaaggag
      661 gccttcgacc ccgccggaat cttcaatccg gcgaaggtgc ttccagaggg ctcacgatgc
      721 ttcgacttcg gcggggtgag acgggagata ccggacgggg cctgggtatg accgagaccg
      781 ccgggggcgg tcccgagcgt gtcgctccct ccaccgtcga ggatctttcc gcgatcctga
      841 aaaaggcgac cgaggaacag cgcctcgtga atgtggtcgg aggcgggacc agacagggct
      901 acggctctcc gcctccccct gacctcgtgc tggagatgag cggtctctcc gggatcgagg
      961 agtgggaccc cgatgacctc accgtgacgg ttcgggcagg aacctccgtc gccgacctgg
     1021 aagctgccct aggcgaacgc tcacagaccg cagtactgcc cgagatcccg ggcacctcca
     1081 ctgtcggagg ggtcatctcg gccgggatct cctcattgcg gagggcacgt ctcctcggca
     1141 ccagggaaag gatgctcgag gttcgcctcg tgaccggaga cggtcgggtg gttcggggtg
     1201 gtggccgggt ggtgaagaac gtctccgggt ccgacctgcc gcgtctagtg attggcgcgt
     1261 tcggctcatt aggggtcatc acctcggtct gtctcaagtt gtggcccatc ccgcctgccg
     1321 cggcgacgat caccatcgat gacatcgggg ttgccgaccg ggtgacgagg ccgctcgccg
     1381 tcctggagcg aaacggcttg accgaggtgt ttctctgggg cacaccagag gaggtcgcag
     1441 cttccggcgg tcgactcggg ggcgcggcga tcgaggggca tcgatggccg gccgacccac
     1501 aggggtcgta ccgatggtcg gttcgagtcc cacccgccct gac
//
LOCUS       DATITW010000551         1887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_27668, whole genome shotgun sequence.
ACCESSION   DATITW010000551 DATITW010000000
VERSION     DATITW010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27668"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VLS86_07670"
     CDS             complement(<1..792)
                     /locus_tag="VLS86_07670"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSO50411.1"
                     /translation="MSSETVEGRVGWIGTGRMGSALVSRLLDAGVEVWVWNRTRSKTE
                     SLAEKGAKVVDKPSDLAVCELVFSMVAGPADVLEVTIGTDGVLNRDDVAPAVLVDATT
                     IDPATSRQLTEEAAHRGTAVLAAPVSGNPKVVKAGKLTVVASGPEAAYNAALPVLQLF
                     GRKVTYVGPGDEARLVKICHNLMLGVVAQSMAEITVLAEAAGVTRSDFLEFLNDSVMG
                     STFTRYKTPAYVNLDYTPTFTWALLRKDLELGLDSSREMGVPLPLT"
     gene            1006..1884
                     /locus_tag="VLS86_07675"
     CDS             1006..1884
                     /locus_tag="VLS86_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSO50412.1"
                     /translation="MLTALFVTVLWASSWVLIRWGLEDGGLAPLSFAGIRYLLASLVL
                     GALVSASPRLRGEITSLTGARWAQLALLGLVFVAVAQGAQFVAIDAQPAATSSLILSL
                     TPLIVAGASPIALAERPTIRLLAGAGLIVLGAAAYFSGDLGFTVVGLAASIVGLVANS
                     IAALLGRGINRGRDMSPIVVTVVSMTIGALALVVTGLSVDGPPAISGRGWVIVIWLAV
                     VNTAWAFTLWNRSLQMLSATESAAINNTMLIQIGVLAWVFLDETPGALDILGMVAVSI
                     GVFLAQRTRAGSTTGP"
BASE COUNT          274 a          637 c          621 g          355 t
ORIGIN      
        1 ggtgaggggg agggggaccc ccatctcccg gctcgagtcg agacccagtt ccaggtcctt
       61 gcgcaacagc gcccaggtga aggtcggcgt atagtcgagg ttgacgtagg ccggggtctt
      121 gtagcgggtg aaggtcgagc ccatgacact gtcgttgagg aactcgagga agtccgagcg
      181 cgtcaccccg gcggcctcgg cgagcacggt gatctccgcc atcgactggg caacgacgcc
      241 aagcatgagg ttgtggcaga tcttgaccag tctcgcctcg tcgcccgggc cgacataggt
      301 caccttccga ccaaacagct ggaggacggg cagggcagcg ttgtaggcgg cctccggccc
      361 ggacgcgacg acggtgagct tgccggcttt gaccaccttc gggttgcccg agacgggcgc
      421 ggcgaggact gccgtcccgc ggtgcgccgc ctcttcggtc agttggcgcg aggtcgccgg
      481 atcgatggtg gtcgcgtcga ccagaaccgc cggtgctacg tcatcgcggt tgagcacgcc
      541 gtcagtgccg atggtgacct ccagcacgtc ggcggggccg gcgaccatgc tgaacaccag
      601 ttcgcacacg gccaggtctg atggcttgtc caccaccttg gcgcccttct cggccagact
      661 ctcggtcttg gaccgggtcc ggttccagac ccagacctcg actccggcgt cgagaagccg
      721 ggagaccagg gccgatccca tgcgaccggt cccgatccaa cccacccgtc cctcgacggt
      781 ctcgctgctc actgactctc cttccaggtg gcgggcggcg cccggaccta tcgacaactg
      841 ccctcgcaaa cgattgcatt gtcgatagcg ctgctctcac tgtcaaacat gagaagcccc
      901 gggatgccag gtggagacct gccattcagc ttccggcaca gatcggcggt ctagcctccg
      961 accaggagat ggtctgacgc caccagccgg gagcacaccg gcgccatcct caccgctctg
     1021 ttcgtcacgg tgctctgggc atcgtcgtgg gtgctgatcc gatggggtct cgaggacggg
     1081 ggcctggcgc ctctttcctt tgccgggatc cgatatctgc tcgcctcact ggtcttgggc
     1141 gccctggtct cggccagccc gagacttcgc ggcgagatca cttccctcac cggcgcgcgc
     1201 tgggcccagc tcgccctgct cgggctggtc ttcgttgctg tggcccaggg cgcccagttc
     1261 gttgccatcg atgctcagcc ggcggcaacg tcgagtctga tcctctccct gaccccgttg
     1321 atcgtcgccg gcgcctcccc catcgccctg gccgagagac cgaccatcag gctgttggcc
     1381 ggggcgggtc tcatcgtcct cggcgccgct gcctacttct ccggtgatct cgggttcacg
     1441 gtggtcggcc ttgcggcgtc gatcgtcggg ctcgtcgcca actcgatcgc cgcgctgctg
     1501 ggtcgtggga tcaatcgggg gcgggacatg tccccgatag tcgtcaccgt ggtcagcatg
     1561 acgatcggag cgctcgcttt ggtggtgaca ggtctgtcgg tggatggacc gcccgcgatc
     1621 tcgggccgcg gctgggtgat cgtcatctgg ctcgccgtgg tcaacaccgc ttgggcgttc
     1681 acgctgtgga accgaagtct gcagatgctc tccgccaccg aatcggctgc tatcaacaac
     1741 accatgctga tccagatcgg ggtcctcgcc tgggtcttcc tcgacgagac tcccggggcc
     1801 ctcgacatcc tcggcatggt cgcggtgtcg atcggggtgt ttctggcaca gcgcacccgg
     1861 gcaggatcga cgaccgggcc gtgagca
//
LOCUS       DATITW010000552         2744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_187343, whole genome shotgun sequence.
ACCESSION   DATITW010000552 DATITW010000000
VERSION     DATITW010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187343"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VLS86_07680"
     CDS             <1..312
                     /locus_tag="VLS86_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50413.1"
                     /translation="HQAEAALVLRYIAETTNLLEAAGNGSVPMRQTEAINDPQLLEIW
                     PFFNDDLKNAYLGSTPTPAGPNAGDVEGILEQMFGEIVVGTDKTAEELAAEYQPQLDA
                     S"
     gene            399..1358
                     /locus_tag="VLS86_07685"
     CDS             399..1358
                     /locus_tag="VLS86_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSO50414.1"
                     /translation="MTATTIQNRAPRGRKFWKRSTKPAPNNNALIGQGKGTPYIFTLP
                     AILLVAAVLGFPVLYGVWQSLFTRETLGSPLEWTGITNYAEMFASPTFWHSMNRTAIF
                     VFGSLILGTTLGLFFSFALFRVVEKLRFLRAITVAPYIISNVAAAVMFRILFNGQFGL
                     LNRTIEWFGFDGPSWLADPTLAMVTVIFTQVWTDLPLTILVLLGGLMAVDRSYLDAAL
                     VDGATGWTRARYLTIPLLAPQLLISTVWLSYSTLTGLGVVLALTGGGPLKATQTMAME
                     MYTVAFRSLEFEAALAIATFILILNALLTLFYVGIGRRYEFDL"
     gene            1355..2290
                     /locus_tag="VLS86_07690"
     CDS             1355..2290
                     /locus_tag="VLS86_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSO50415.1"
                     /translation="MSDRHREEEPAFAVATARATGAVTGPPPEGRRAPGGRVRRGLRQ
                     FLLMLPLVALVVWTLAPFLVTMSVSFKERADVFANPGLIPSNPTLDAYREVLTSDSFV
                     ASFLNSLVVGIGTTLLTLVIGVPAAYAFARFNFRGRHFLLLFTLLPRLVPSLGLMVPI
                     YRLAVAAGLLDKRITLVIVYTGMLLPLAVWLMVGFFQQIPREIEEAANVDGATLWTRL
                     RYIVAPLAAPAMITIGVLAFREAWNEFTLVLVLTSSPGKRTLPFELYLMQGIEGIANF
                     PGEAAFTLLTVLPFVLVYTRIEKYVVAGLVTGSGK"
     gene            2310..>2744
                     /locus_tag="VLS86_07695"
     CDS             2310..>2744
                     /locus_tag="VLS86_07695"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HSO50416.1"
                     /translation="MLDKLIITAACDSSVSYPSNPNCPSPTRENTAPIADEYIRCIDA
                     GAAISHLHGVRKLETELQADGRKLSRIDVEGWAEITDRIRSSRDTIIQFGIAGARFED
                     RVPLLDLSPEMMSVAFNAHDEHFQPDPEYPPNCIYSLHPIDEL"
BASE COUNT          461 a          944 c          818 g          521 t
ORIGIN      
        1 catcaggccg aggcggcgtt ggtccttcgc tacatcgccg agaccaccaa cctgctcgag
       61 gccgcgggga acggctcggt gccgatgcgt cagaccgagg cgatcaacga cccgcaactc
      121 ctcgagatct ggcccttctt caacgacgac ctgaagaacg cctacctcgg gagcacgccg
      181 acgccggccg ggcccaatgc cggggacgtg gaagggatcc tggagcagat gttcggcgag
      241 atcgtcgtcg gaaccgacaa gacagcggag gagttggccg cggagtatca gccgcaactc
      301 gacgcctcgt aagcaagaat cacgactgaa ggaagcgcac ccgtgtccac cgatgcgggt
      361 gcgctcctca ccaaccgaag ccaaccggtg actagtcgat gaccgcgacc acgatccaaa
      421 accgagcgcc acgtggtcgc aagttctgga agcgcagcac caaaccggcc ccgaacaaca
      481 acgcgctcat aggccagggc aagggcacgc cgtacatctt caccctcccg gccatcttgc
      541 tggtggccgc ggtgctcggc ttcccggtcc tctatggggt ctggcagagc ctgttcacca
      601 gagagaccct cggttctccg ctggagtgga cggggatcac caactacgcc gagatgttcg
      661 cctcgcccac gttctggcac tcgatgaacc ggacggcgat cttcgtcttc gggtctctca
      721 tcctcgggac cacgctcggg ttgttcttct cgttcgccct gttccgggtg gtcgagaagt
      781 tgcggttctt gagggccatc accgtcgccc cctacatcat ctccaacgtc gcggccgcgg
      841 tcatgttccg catcctgttc aacggccagt tcgggctcct caaccggacc atcgagtggt
      901 tcgggttcga cggtccctca tggctggccg accccacact cgccatggtc accgtgatct
      961 tcacccaggt gtggaccgat ctcccgctca ccatcctggt cctcctcggc gggttgatgg
     1021 ctgtggaccg gtcctatctc gatgccgccc tcgtcgacgg agcaaccgga tggaccaggg
     1081 ccaggtacct cacgatcccg ctgctcgcgc cccagctgct catcagcacg gtttggctca
     1141 gctactcgac gctgaccggg ctgggtgtgg tgcttgctct caccggcggt ggcccgctta
     1201 aagccaccca gaccatggcg atggagatgt acaccgtggc gttcaggagc ctcgagttcg
     1261 aggcggcact ggccatcgcc accttcatcc tcatcctcaa cgccctgctc accctgttct
     1321 acgtgggcat cggcagacgc tacgagttcg acctatgagc gacaggcacc gggaagaaga
     1381 gccggcattc gcggtcgcca ccgcgagagc gacaggggcg gtcaccggtc cgccgcccga
     1441 aggtcgccgt gcccccggcg gcagggtccg ccgcgggctc cgtcagttcc tgctcatgct
     1501 tcccctggtg gcgctggtgg tctggaccct cgccccgttc ctggtcacga tgtcggtgtc
     1561 gttcaaggaa cgggccgacg tcttcgccaa cccggggttg atcccttcca accccactct
     1621 cgacgcctac cgcgaggtcc tgaccagcga cagcttcgtc gcatcgtttc tcaacagcct
     1681 cgtcgttggc atcggcacca cattgctgac cttggtgatc ggtgttcccg ccgcctatgc
     1741 cttcgcccga ttcaatttcc ggggacggca tttcctcctg ctgttcaccc tgctgccgcg
     1801 gctggtgccc agtctggggc tgatggtccc gatttacaga ctggcggttg ccgccggcct
     1861 tctcgacaag cggatcaccc tggtcatcgt ctacacgggg atgctcttgc ccctggcggt
     1921 gtggttgatg gtgggtttct tccagcagat accgcgcgag atcgaggagg cagccaacgt
     1981 ggacggggcg acattgtgga cccggctccg ctacatcgtc gctcccctgg cggcgccggc
     2041 aatgatcacc atcggtgttc tcgcgttccg ggaggcgtgg aacgagttca ccctggtcct
     2101 ggtgctcacc tcctcccccg gcaagcggac gctccccttc gagctctacc tgatgcaggg
     2161 gatcgagggc atcgccaact tccccgggga ggctgccttc accctgctca ctgtgctccc
     2221 gttcgtgttg gtctacaccc ggatcgagaa gtacgtggtc gccggcctgg tgacaggctc
     2281 gggcaagtaa gacgctcgtc aggagacgca tgttggacaa gctgatcatc accgccgcct
     2341 gtgactcctc ggtctcctat ccgtcgaatc cgaactgccc atcgccgacc agggagaaca
     2401 cggcgccaat cgccgatgag tacatcaggt gcatcgatgc cggggccgcc atctcccacc
     2461 tccatggcgt ccgcaagctg gagaccgagt tgcaggccga cggtcgcaag ctgagtcgga
     2521 tcgatgtgga gggctgggcc gagatcaccg accggatcag atccagcagg gacaccatca
     2581 tccagttcgg gatcgccggg gcccgattcg aggatcgggt gccgctcctc gacctctccc
     2641 cggagatgat gtcggtcgcg ttcaatgccc acgacgagca cttccagccc gaccccgagt
     2701 atcccccgaa ctgcatctac tcgctgcatc ccatcgacga gttg
//
LOCUS       DATITW010000553         2064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_187420, whole genome shotgun sequence.
ACCESSION   DATITW010000553 DATITW010000000
VERSION     DATITW010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187420"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VLS86_07700"
     CDS             complement(<1..245)
                     /locus_tag="VLS86_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HSO50417.1"
                     /translation="MTLYWAEALDNTVIWTAETLDRRVVMETLYKQEASSLVRMARLF
                     TDDRTAAEDLVQEAFIRLFHSAHRIDDPGKAAAYLRS"
     gene            complement(242..625)
                     /locus_tag="VLS86_07705"
     CDS             complement(242..625)
                     /locus_tag="VLS86_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50418.1"
                     /translation="MSWWAIELATNLILLALVLFLGPLIKRFGRSYAGDVFRGNPRTG
                     KSYLVLMDVAYYLIFFAYILFTVSFIDQSLTLGAQLEEQLTRIGGMLLLMGVLHGLNV
                     LTLPIVGRLLGLSRQLEEDTPPPAA"
     gene            complement(651..1247)
                     /locus_tag="VLS86_07710"
     CDS             complement(651..1247)
                     /locus_tag="VLS86_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50419.1"
                     /translation="MSRLDRLAAAGVGIAIPAAMVLGAMLGGVAALAVLLVAVAVGWR
                     YVPGFLRTIAQGFLAGVIAGVLILGPGFRLAMRVVAIFDIRRVEFTLEGTLFIILFIG
                     GVVGGAVGIAAVFLRRGLGWSPGLMTGVMAVAIMSLLLIDTGLRSELVELGAGPWLNI
                     PMFTAVTIGYGLATNRLIDRFAARRSRRETREPVKVPK"
     gene            complement(1354..>2064)
                     /locus_tag="VLS86_07715"
     CDS             complement(1354..>2064)
                     /locus_tag="VLS86_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSO50420.1"
                     /translation="LEMQRELGDPKEIANALYNHGLVVAFTAGRVAEDIELEDLEAIF
                     DEAESIYLELGDVGGLGNIEWARGTAVAFILENPDEASEHMKKAIDYYSQAGNEFGMG
                     WGLFEVGEMARRSERFEEAWEYLRRGLALFESHGDVSAAVLFIAGIAGVAQGLGDMQR
                     AARLAGAFTTLRITSGTDLVDYETNQVSGLDFESLEALTGDLALAYRNGRAMGFDQAV
                     TYALAGPVDEGFETQRAQ"
BASE COUNT          410 a          706 c          597 g          351 t
ORIGIN      
        1 atcgatcgga ggtacgccgc cgccttgccc ggatcgtcga tgcgatgagc cgagtggaac
       61 aaccggatga acgcctcttg gaccaagtcc tcggcggcgg tccggtcgtc ggtgaacagc
      121 cgggccattc gaaccagaga agacgcttcc tgtttgtaca gcgtctccat gacgacgcga
      181 cgatccagcg tctccgcggt ccagatcacc gtgttgtcga gcgcctcagc ccagtacaag
      241 gtcaagccgc aggtggtggg gtgtcctctt cgagctgacg gctgaggccc aagagacgtc
      301 ccactattgg cagggtcaac acgttgagcc catggagcac ccccatcaac agcagcatgc
      361 cgccgatcct cgtcagttgc tcctcgagtt gagccccgag ggtcagggat tggtcgatga
      421 agctgacagt gaacaggatg taggcgaaga agatgaggta gtaggcaaca tccatcaaca
      481 ccaggtagct cttaccggtg cgggggttgc ctcggaacac atcgccggca taactgcgcc
      541 cgaatcgctt gatcagcggc cctaagaaca ggaccagcgc caacaggatc agattggtcg
      601 ccagctcgat ggcccaccag ctcatgacgg ttcgactctc atgctcggtg tcattttggc
      661 accttgaccg gctcccgggt ctcccgacgc gatctcctgg ccgcgaagcg gtcgatcaac
      721 cggttggtgg ccaacccgta gccaatggtc acggcggtga acatcgggat attgagccac
      781 ggcccggcgc ctagttcgac gagctccgag cgcagcccgg tgtcgatcag gagcagactc
      841 atgatcgcga cggccatcac cccggtcatc agccccggtg accatcccag gcctcgtctc
      901 aggaatacag ccgcgatccc aaccgccccg ccgacgaccc caccgatgaa caggattatg
      961 aacaaagtgc cctcaagcgt gaactcgacc cgccgaatgt cgaagatggc gacaacccgc
     1021 attgccagcc ggaatccggg tcccaggatg agcactcccg cgatcacgcc ggcaaggaag
     1081 ccttgggcaa tcgtcctcag gaagccgggg acatatcgcc agccgaccgc caccgctacc
     1141 agcagcacgg ccaatgctgc cacgccgccc aacatcgctc ccagaaccat cgccgccggg
     1201 atggcgatcc ccactcccgc ggcggcaagc ctgtccaatc tcgacatgag tactgctccc
     1261 tgatcgtttc gatagacaga cgaagaggag tcgaaaacgg gtgacagccg tcggctcggg
     1321 aatcgccctc accgcccagc aggttgcctg gctctattgg gctcgctgag tctcgaagcc
     1381 ttcgtccacc ggcccggcca gcgcgtaggt gaccgcctgg tcgaacccca tcgcacggcc
     1441 gttgcgatag gccaacgcca ggtccccggt gagagcctcg agactctcga aatcgaggcc
     1501 cgacacctga ttggtctcgt aatcgaccag gtcggtcccg gaggtgatcc ggagagtggt
     1561 gaacgcgccg gcgagtcgag ccgcccgttg catgtcaccg agaccctggg ccacaccggc
     1621 gattccggcg atgaagagca cggcggcgga gacatcaccg tgactctcga acagggccag
     1681 gcctcgccgc agatactccc aggcctcttc gaatcgctcg ctgcggcgcg ccatctcgcc
     1741 gacctcgaac agcccccaac ccattccgaa ctcattgcca gcctgcgagt agtagtcgat
     1801 cgccttcttc atgtgctcgc tggcctcgtc gggattctcg aggatgaaag ccactgcagt
     1861 cccccgcgcc cattcgatgt tccccaatcc gcccacatcg cccagctcga ggtagatgct
     1921 ctccgcctcg tcgaagatgg cctccaggtc ctcgagctcg atgtcctcgg ccaccctccc
     1981 tgctgtgaac gccaccacca gcccgtggtt gtagagggcg ttggcgatct ccttggggtc
     2041 acccagctcg cgttgcatct ccag
//
LOCUS       DATITW010000554         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_387906, whole genome shotgun sequence.
ACCESSION   DATITW010000554 DATITW010000000
VERSION     DATITW010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387906"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VLS86_07720"
     CDS             complement(<1..347)
                     /locus_tag="VLS86_07720"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HSO50421.1"
                     /translation="MSLIAFVMAAASLLIVEETANAAIVPTVPLGTSANFSVLGSTTV
                     TNTGDSVLAASAGVWAGTAITGFPPGIVQPPGTIEAGTPVAQQAQADLTAAYLNAEGR
                     PINQTLTADISGLT"
     gene            590..874
                     /locus_tag="VLS86_07725"
     CDS             590..874
                     /locus_tag="VLS86_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSO50422.1"
                     /translation="MRDWSFLTNHARALLFIAHDPDSRLRDLAVALDVTERTAYGIVV
                     DLTQAGYVAKEKDGRRNRYHVQSHLPLRDSIGRQRAIGELVDLFGMSPKH"
     gene            936..1922
                     /locus_tag="VLS86_07730"
     CDS             936..1922
                     /locus_tag="VLS86_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HSO50423.1"
                     /translation="MTSDPSQTLPIVDPAPSGMRWIPGGRFLMGSENFYPDEGPVHEV
                     EVDGFWMDAHPVTVAEFRRFVRETDYVTVAERPLDPDEYPDAIRELLVPGSLVFRKTK
                     GPVDLNDYRNWWEYVPGANWRRPGGPGSTIHSRTDHPVVQVALEDVETYANWAGKELP
                     TEAEWEFAARGGLEGATFAWGDEHFPDGKPMANSWQGEFPWQNLKLDGFEGTSPVGSF
                     PSNGYGLYDMTGNVWEWTSDWYVPRHPDEVASPCCVPSNPRVTSRDESYNPGQPGEHI
                     PRRVIKGGSHLCAPNYCLRYRPAARQPQTIDTSMAHLGFRCVVRPKIPTTAH"
     gene            complement(1879..>2078)
                     /locus_tag="VLS86_07735"
     CDS             complement(1879..>2078)
                     /locus_tag="VLS86_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50424.1"
                     /translation="HRFSAMRRHQQDEGSTSSGGDYIIDPSELSGVFNVPVSSTQLIS
                     PENTLLLFSERWSGSWVGRRI"
BASE COUNT          388 a          667 c          663 g          360 t
ORIGIN      
        1 gtgagaccgc tgatatcagc cgtcaacgtc tggtttatcg ggcggccttc cgcattgaga
       61 tacgcagcag tcagatccgc ttgcgcctgt tgggcaaccg gagtgcccgc ctcaatcgtt
      121 cctggaggct ggacgatccc aggtgggaat cccgtgattg cggtaccggc ccagacgccg
      181 gcactggccg caagcacact gtcgccggtg ttggtgaccg ttgtggaccc caataccgag
      241 aagttggccg aggtccccaa ggggactgtg ggcacgatgg ccgcgttcgc ggtctcttca
      301 acaatgagta gagacgctgc cgccatcacg aacgcgatga ggctcacgcc tatacggcgg
      361 gctgtcgggc gaggggagcg agtggcagga ctccgtcgca gctggtgcag accgatcaag
      421 acggttgcac tgagagcgat tcccacgatg agcacgcggg ctccttccta gaccgggata
      481 caggagccgc cgtcggcgac acgggtgccg gccaagtggt tcaagcatat tccggcagtc
      541 tgaaactagc aaatcgcttc cggaattgat gtactactat cctcgagcca tgcgtgactg
      601 gagcttcctg acgaaccacg ctcgggcgtt gttgttcatc gcccacgacc ccgattcgcg
      661 gctccgcgac ctggctgtgg ccctcgatgt caccgaacgc accgcctatg ggatcgtggt
      721 cgacctcact caggcgggtt acgtggcgaa ggagaaggac ggtcggcgca atcgctacca
      781 cgtgcagtct caccttccgc tgcgcgactc gatcgggcgt cagcgcgcca tcggcgaact
      841 ggtggatctc ttcggcatga gcccgaagca ctgaggactc ccgggtcccg cctgcctgag
      901 tatgccggcg gccgatttcg ctaggttcgc ttcagatgac atcggaccca tcacagacgc
      961 tgccgatcgt cgatccggcg ccatccggca tgcgctggat cccggggggc cgcttcctga
     1021 tggggtctga gaatttctat cccgacgaag gaccggtaca cgaggtcgag gtggacgggt
     1081 tctggatgga cgcgcacccg gtcaccgtcg ccgagttcag gaggttcgtc cgagagaccg
     1141 actatgtcac cgtggccgag cggccgctcg atccggacga gtatcccgac gccatccggg
     1201 aactcctcgt gcccgggtcc ctggtgtttc gcaagaccaa gggaccggtc gacctcaacg
     1261 actaccgcaa ctggtgggag tacgtgcccg gcgccaattg gagacgaccc ggcggccctg
     1321 gcagcacgat ccacagccgg accgatcacc cggtggtcca ggtcgccctc gaggacgtgg
     1381 agacctatgc gaactgggcc gggaaagagc ttcccacaga ggcagaatgg gagttcgcgg
     1441 cacgtggggg cttggagggc gcaaccttcg cctggggaga cgagcacttc cccgacggca
     1501 agcccatggc caacagctgg caaggcgagt tcccctggca gaacctgaag ctcgacgggt
     1561 tcgagggaac ctcccctgtg ggtagcttcc cgtccaacgg gtacgggctg tatgacatga
     1621 ctggaaacgt ctgggaatgg acctcggact ggtacgtgcc gcgccacccc gacgaggtcg
     1681 cgagtccgtg ctgcgtcccg tcgaaccccc gcgtcacctc acgcgacgag agctacaacc
     1741 caggccaacc cggcgagcac atccctcgcc gtgtgatcaa gggcggctca cacctctgcg
     1801 cgccgaacta ctgcctccgt taccgccccg cagcgcgaca gccgcagacg atcgacacgt
     1861 cgatggcgca cctcgggttc agatgcgtcg tccgacccaa gatcccgacc accgctcact
     1921 gaaaaggagc agcgtgttct caggagagat caactgggtc gagctcacgg gcacattgaa
     1981 gacgccgctg agctcgctcg gatcgatgat gtagtcgccg cctgagctgg ttgagccctc
     2041 gtcctgctgg tggcgtctca tcgccgagaa cctatgct
//
LOCUS       DATITW010000555         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_748512, whole genome shotgun sequence.
ACCESSION   DATITW010000555 DATITW010000000
VERSION     DATITW010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748512"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..591
                     /locus_tag="VLS86_07740"
     CDS             100..591
                     /locus_tag="VLS86_07740"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HSO50425.1"
                     /translation="MTSDLQVPVHDLIGNPGKQRPFAGEKPVLLRLGDTVVDGPMRVS
                     GEVSGLIDAVTAHFTVSADAHLTCTRCLTEWDETMTAEGDQVYRRVPDEDGYGIVDGE
                     IDISGPAQDELALSLPIVPLCKPDCKGLCPTCGTDLNTEPCDGHGEDPDSPFAVLKDL
                     FDS"
     gene            613..789
                     /gene="rpmF"
                     /locus_tag="VLS86_07745"
     CDS             613..789
                     /gene="rpmF"
                     /locus_tag="VLS86_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HSO50426.1"
                     /translation="MAVPKKKMSRSRTRQRKAVWKVSRPTAVACEQCGKPKLPHRACR
                     ECGTYKGREVVKAD"
     gene            794..1765
                     /gene="plsX"
                     /locus_tag="VLS86_07750"
     CDS             794..1765
                     /gene="plsX"
                     /locus_tag="VLS86_07750"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HSO50427.1"
                     /translation="MARIALDAMGGDNAPTETVAGAVAASKRGVDVVLVGEHVALEAE
                     LAKHETRLPIVDAPEVIGMSDDPARSLREKPGASITVAAKLVEEGSAAGLVSAGSTGA
                     AMAAAAIIIGRIKGVSRPSIASLFPTPGSPTLVLDSGANPDVRPEQLAQFGIMGSVAA
                     QVMLGLAEPRVGLLSNGEEKGKGRDLERAAYDLLESASINFIGNVEGRDVATDRADVM
                     VTDGFTGNILLKTTEGAVQIVALYLLEELSKLDPEVQEAVMPAIFRVRQRLDWETYGG
                     AQLLGIKGVVVIAHGASSQVAIANALVLARDSADRDLPGRLAVELAL"
     gene            1762..>2219
                     /gene="rnc"
                     /locus_tag="VLS86_07755"
     CDS             1762..>2219
                     /gene="rnc"
                     /locus_tag="VLS86_07755"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HSO50428.1"
                     /translation="MTSLDDELGYVFTGSELLSLSLTHRSVSAEDPSRHDNERLEFLG
                     DAVLQLVITDRLYRDYPRLAEGQMAKIRAALVSRPTLADVARTIDLGNHIELAPAEER
                     SGGRNKDSILADAMEAVIGAVYLDGGIEQVSKLIERLWGDRVGERAKSPGV"
BASE COUNT          404 a          722 c          730 g          363 t
ORIGIN      
        1 gggtggaggg tctgctggcc gagatcacgg accaggtgca gacggctcga gacgagctgc
       61 acacggctcg ccccaagccg cccgacgtcc cgatctgaga tgacgtccga cctccaggtc
      121 ccggtccacg acctgatcgg caacccgggc aagcagcggc cgttcgccgg tgagaaacca
      181 gtcctcttgc gtctcggcga caccgtggtc gacgggccga tgagggtcag tggcgaggtc
      241 tcgggtctca tcgacgcggt gaccgctcat ttcacggtct ctgccgacgc ccatctcacc
      301 tgcacccgtt gtctgaccga gtgggacgag accatgaccg cggagggaga tcaggtctat
      361 cggcgagtgc cggatgagga cggatatggc atcgtcgacg gggaaatcga catctccggg
      421 ccggcccagg acgagctggc gctcagcctc ccgatcgtcc cgctttgcaa accggactgc
      481 aagggacttt gcccaacgtg tggaaccgac ttgaatacgg agccctgcga cggtcatggg
      541 gaggatcccg attcgccttt cgccgtcctg aaagatctgt tcgattcctg acctatcaag
      601 gagtgcccgc aaatggcggt gcccaagaag aagatgtcgc gctcgcggac ccggcaacgg
      661 aaagctgtct ggaaggtgtc ccggccgacc gctgtcgcct gtgagcagtg cggcaagccc
      721 aagctccctc accgcgcctg tcgtgagtgc ggcacctata agggccgcga ggtcgtaaag
      781 gcagactgag gctttggcac gcatcgccct cgacgcaatg gggggcgaca acgccccaac
      841 cgagaccgtc gctggggcgg ttgcagcctc caagcggggt gtcgacgtgg tactagtcgg
      901 cgagcacgtg gcgctcgagg ccgagctggc caagcacgag acccgtctgc ccatcgtcga
      961 cgcccccgaa gtcatcggga tgtccgacga tcctgcccgc tcccttcgag agaagccggg
     1021 cgcctcgatc acagtcgcgg ccaaactggt cgaagagggc tcggctgcag gcctggtgtc
     1081 cgctggatcg acaggcgctg ccatggcggc ggcggcaatc atcatcggga ggatcaaggg
     1141 tgtttcccga ccgtcgatcg cgtcgttgtt tcccaccccc ggatcaccca ccctggtgct
     1201 ggactcggga gccaacccag acgtcagacc tgagcaactc gcccagttcg gcatcatggg
     1261 ctcggtggcc gcccaggtga tgctcggctt ggccgagcca cgggtcggcc tgctctcgaa
     1321 cggcgaggag aagggcaaag gacgcgacct ggagcgcgcc gcctatgacc tcctggaaag
     1381 cgcctcgatc aatttcatcg ggaacgtcga gggacgcgat gtcgccaccg accgcgccga
     1441 cgtgatggtc acggacgggt tcaccgggaa catcctgctc aagaccaccg agggggcggt
     1501 tcagatcgtc gccctctacc tcctggagga gctgtcaaaa ctcgaccccg aagtccagga
     1561 ggcggtgatg cccgcaatat tccgggtgcg tcagcgtctc gattgggaga cctacggtgg
     1621 agcccagctc ctcgggatca agggtgttgt cgtcatcgcc cacggcgcct cgagtcaggt
     1681 cgccatagcc aacgccctcg tcctcgccag ggacagtgcc gatcgcgacc ttcccggtcg
     1741 tctcgccgtc gagctggcct tgtgacgtcg ctggacgacg agctcggcta cgtattcacc
     1801 ggctccgagc tcctcagcct ctcgctcacc catcgctcgg tcagcgccga agatccgagt
     1861 cgccacgaca acgaaagatt ggaattcctg ggcgacgccg tgctccagtt ggtgatcacg
     1921 gaccgtctct atcgcgacta cccccgtctg gccgaggggc agatggccaa gatccgcgcc
     1981 gccctggtga gccgccccac actcgccgat gtggcgcgaa ccatcgacct tggaaatcac
     2041 atcgagttgg ccccagccga ggagcgctcc gggggccgga acaaggattc catactcgcc
     2101 gatgccatgg aagccgtgat cggggcggtg tacctggacg ggggcatcga gcaggtgagc
     2161 aagctcatcg agcggctctg gggagaccgg gtgggggaga gggccaagag ccccggggt
//
LOCUS       DATITW010000556         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_308069, whole genome shotgun sequence.
ACCESSION   DATITW010000556 DATITW010000000
VERSION     DATITW010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308069"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VLS86_07760"
     CDS             <1..854
                     /locus_tag="VLS86_07760"
                     /inference="COORDINATES: protein motif:HMM:NF020287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HSO50429.1"
                     /translation="ARPPGRGDCHPDMNLEPGDPALLIDSKGRHFLLKLEPGRMFQYH
                     EGMVAHSELIGREDGTWVEASTGAHLLLLRPRLADFILKMKRSAQVVYPKDLGPILVY
                     ADIGPGMTVLEAGTGSGALTLGLSRAVGPTGKVVSVELRDDHAAHARKAVERWYGSLP
                     ANIDLRVGDVADHVAEVAPDRIVLDLPEPWHVLDVAAEHQPNGGVLAAYLPTVPQVQT
                     LVETARATGAFAEIEVKEFLFRDWNVSGRSVRPEHNMIGHTGFLTFMRKTAVRQVAPG
                     GPVAAPG"
     gene            890..1135
                     /locus_tag="VLS86_07765"
     CDS             890..1135
                     /locus_tag="VLS86_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HSO50430.1"
                     /translation="MKVWIDQDLCTGDGLCEEIAPDVFVLLDDGLAYVREGPLIFAES
                     EGNPQGAVGVAAVPGGQEEITIESAEECPGECIFIEA"
     gene            1302..2750
                     /gene="arc"
                     /locus_tag="VLS86_07770"
                     /pseudo
     CDS             1302..2750
                     /gene="arc"
                     /locus_tag="VLS86_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
BASE COUNT          542 a          866 c          946 g          479 t
ORIGIN      
        1 gtgctcggcc tcctggtcgg ggcgattgcc atcctgacat gaatctcgag cccggcgatc
       61 cggcgcttct catcgattcc aaggggcgcc attttctgct caagctggag ccgggccgga
      121 tgttccagta ccacgaggga atggtggccc attccgagct catcggacgg gaggacggga
      181 cctgggtcga ggcgtccacc ggcgcccatc tgcttctgct ccgcccccgg ctggctgact
      241 tcatcctcaa gatgaagcgg agcgcgcaag tcgtctaccc aaaggacctg gggccgatcc
      301 tggtgtacgc cgacatcggg ccggggatga ccgtgctcga ggccggcacc gggtcggggg
      361 ccttgaccct cgggctgagc cgagcggtcg gccccaccgg caaggtggtg agcgtcgagc
      421 tccgtgacga tcacgccgcc catgcccgca aggccgtcga gcgctggtac gggtcacttc
      481 cggcgaacat cgatctgcgg gtgggtgacg tggccgacca tgtggccgag gtcgctccgg
      541 atcggatcgt cctcgatctc cccgagccat ggcacgtcct ggacgtcgcg gccgagcacc
      601 aacccaatgg tggtgtgctc gccgcctacc tacccaccgt gccccaggtg cagaccctgg
      661 tggagacagc ccgggccact ggtgcctttg ctgagatcga ggtcaaggag ttcctgtttc
      721 gcgactggaa cgtgtcgggg cgctcggtgc ggccggagca caacatgatt ggccacaccg
      781 gctttctgac attcatgagg aagacggcgg tgcgccaggt cgcccccgga gggccggtcg
      841 cggcccctgg ttagcatcga ccgcggatgc gcgttctcgt ggatgaatga tgaaggtctg
      901 gatcgaccag gatctctgca ccggtgacgg cctgtgcgag gagatcgcgc ccgatgtctt
      961 cgtgcttctc gacgatggtc tcgcctacgt gcgcgagggg ccgctgatct tcgccgagag
     1021 cgagggcaac ccgcaggggg cggtaggagt cgccgccgtg cccggcggcc aggaggagat
     1081 cacgatcgag tcggccgagg agtgccccgg cgagtgcatc ttcatcgagg cctgaaccag
     1141 cgatcagtcg ctcaagtccc ggggtcggtc cgccgaccct tcttgtttgt cggtgagaag
     1201 tgccattatc gggggactgg tcattgggcc ggagaaccca aagaactaaa gagacctatt
     1261 cgtatactgc tcttgaggcg accaaagcca ggaggaagcc cgtggacgaa ggacggagac
     1321 cggacgacgc cactcagttg cgcggtgtga tcaccgcgct cgaggaggaa atcgccgtcc
     1381 tgcgccgtcg tctgcgcgag gcccccgccc gtgtcgacga gttggagagc gagctgtccc
     1441 ggatgaggga gagactcgac cgagcgatgt cccagaacga caagctctcc gcagtgctcg
     1501 aagaggcgcg ggagcaactc ggtgtcttgc gggaagaggt agagaagctg accgccccac
     1561 ccaacaactt cgggacggtc ctccaggtga acgacgacgg cactctcgac gtcatgaccg
     1621 ggagtcgcaa gcttcgggtg gcggcgcagc caaacgtcga ggtcaagaag ctccaggtgg
     1681 gccaggaggt cttgctcaac gaggcgttca acatcatcga cttccgccat ttcgagcctg
     1741 ccggcgacgt gatgagggtg cgcgaggtgc tggccgacgg tcgggtggtc gtgttcgccc
     1801 acgccgacga ggagcgagtc ctgcagcgct ccgaggccat ggccgacgtg aagctccgcc
     1861 ctggtgagta cgtgcgggtc gactccaaga ccggtctcgt cctggagaag atggaacgac
     1921 ccgaggtcga ggagctggtg ctcgaggaag tgcctgacat cacctactcc gatgttggtg
     1981 gtttggcgga ccagatcgag cagatccggg acacggtcga gctgccctat ctccataagg
     2041 atctgttcaa ggagtacgag ctggagccac ccaagggcat cctgctctac ggccctcccg
     2101 gatgtggcaa gaccctcatc gccaaggcgg tggccaacag cctggcgaag aaggtgtcgg
     2161 agcgaaccgg cagccccgac gtccgctcct acttcctcaa catcaagggt cccgagctgc
     2221 tcaataagtg ggtgggtgag accgagcgcc agatcaggct gatcttacaa agggcaaagg
     2281 agcagtcaga cgagggagtt cccgtaatcg tgttcttcga cgagatggac tcgctgttcc
     2341 gcaccagggg aaccgggatc agctccgatg tcgaatccac catcgtgccc cagctcctct
     2401 ccgagctcga cggcgtcgaa tcactgaaga acgtggtggt gataggcgcc tcgaaccgtg
     2461 aggtcctgat cgatgctgcc atcctccgcc cgggacggct cgacgtgaag atcaagatca
     2521 accggcccga cccgatcgca gccagggaga tcttccgcat ctacatcagc gacgagaccc
     2581 ccatcgacgc cgaggagatc gagagacagg gtggggacaa gacaaaggtg atcgctgact
     2641 acatcgaggc cacggtccag gccatgtact cggagtccga cgagaaccga ttcctcgagg
     2701 tcacatactc ctccggagac cgtgagctgt tgttcttcag ggactactca tagggcgcca
     2761 tcctcgagaa catcgtgagg cgggccaaca aagatgcgat caagcgccac gtcggcggcg
     2821 gcgagccagg ggt
//
LOCUS       DATITW010000557         1592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_147646, whole genome shotgun sequence.
ACCESSION   DATITW010000557 DATITW010000000
VERSION     DATITW010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147646"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1592
                     /locus_tag="VLS86_07775"
     CDS             <1..>1592
                     /locus_tag="VLS86_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50431.1"
                     /translation="RGFWRTLAIGFWGFAIAAFWALPLAARIGLTSDMAWSPLTRWEE
                     VFPVELWLLLPVAIPGAIWASRHTSRAGPLMAATLLPLIYVPLPTVLPELFPGLFGGE
                     RWKLWNGRLLPYWYFGVAFFAAVGVGAAAVWASRRLPSRVSTHWARLLIAVATVVAAA
                     LVADSADFPAWAWIPVSMAGAGLVVLSFFWSPDLSTRHFLTASVTAVIVMGALAGVTF
                     IDGWSKWNYEGYESKEPWPEYEALMMRLDELPDGRVHWEANNEALNRYGTPMSPMLIP
                     YWTEGSHKSMEGLFFESSLTTPFHFINSSEMSEKPSNPIPGLTYHTGEMDRGIKHLDL
                     YGVSYYVAVTPEATAKADGMPELTKLVTTPPFTIYALPENSLVEPASFQPSVYDVEDR
                     GLLALLTGHATVTDDDGVTLPSFYDMSLEWYDDVDNLDRWVVADGPEDWPRIESLDER
                     STQALRVPTDAVSNVVVEDHRISFHTTAVGVPHLVKVSYFPNWIAEGADGPWRAAPSL
                     MVVVPTSEDVTIEFENTWAETG"
BASE COUNT          251 a          497 c          504 g          340 t
ORIGIN      
        1 gtcgaggctt ctggcgcacc ctggcaatcg gattctgggg tttcgccatc gccgcgttct
       61 gggcgctgcc gcttgcggcg cggatcgggc tcacgtcgga catggcctgg tctccgctca
      121 ctcgatggga ggaggtcttt cccgtcgagt tgtggcttct cctcccggtg gcgataccgg
      181 gcgccatctg ggcgagccgt cacacctcgc gtgccgggcc cctgatggcg gcgaccctgc
      241 ttccgctcat ctacgtccca ctccccacag tcctacccga gttgttccct ggattgttcg
      301 gtggtgaacg gtggaagctg tggaacggga gacttctccc ttactggtat ttcggagtcg
      361 cgttctttgc cgccgttggt gttggtgcag ccgcggtctg ggcctcccgg cggcttcctt
      421 cccgggtctc gacccattgg gcgcgtttgc tgatcgccgt cgccaccgtg gttgccgcgg
      481 cactggtggc cgactctgcc gatttcccgg cgtgggcctg gatcccggtg tccatggccg
      541 gtgcggggtt ggtggtcctc tccttcttct ggtcgcccga tctcagtacc cgtcattttc
      601 tgaccgcgtc ggtcactgcc gtgatcgtga tgggggcact cgccggcgtc accttcatcg
      661 acgggtggtc caagtggaac tacgagggct acgagtcgaa agagccctgg cctgagtacg
      721 aggctctgat gatgcgtctc gacgagctac cggacgggcg agttcactgg gaagccaaca
      781 acgaggcgct caaccggtac gggactccga tgtcgccgat gctgatcccg tattggaccg
      841 agggctccca caagtcgatg gagggcttgt tcttcgagtc ttcgctgaca acacctttcc
      901 acttcatcaa ctcttcggag atgtccgaga agccctccaa cccgatcccg ggactcacct
      961 atcacaccgg ggagatggac cgtgggatca agcatctcga tctctacggc gtgtcgtact
     1021 acgtggcggt cacaccggag gcaaccgcca aggcagatgg gatgcctgag ctcaccaagc
     1081 tcgtgacgac ccctccgttc acgatctatg cgctgccgga gaacagcctc gtcgagcccg
     1141 catcctttca gccctcggtg tacgacgttg aggacagagg attgctggcg ctgttgaccg
     1201 gtcatgcgac cgtcaccgac gacgacggtg tgaccctgcc cagcttctac gacatgtcac
     1261 tcgagtggta cgacgacgtg gacaatctcg accggtgggt cgttgccgac ggcccagaag
     1321 actggccgcg tatcgagtcg ttggatgagc gctccaccca ggcgctgcgg gtgccgacag
     1381 acgcggtgtc aaacgtggtc gtcgaggatc accgaatcag ttttcacacg acggcagtcg
     1441 gtgtccctca cctggtcaag gtctcgtatt tcccgaactg gattgccgaa ggcgctgatg
     1501 ggccgtggcg ggcggccccg tcattgatgg tggtggtacc caccagcgaa gatgtgacca
     1561 tcgaattcga gaacacctgg gcggagactg gg
//
LOCUS       DATITW010000558         1631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_468330, whole genome shotgun sequence.
ACCESSION   DATITW010000558 DATITW010000000
VERSION     DATITW010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468330"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="VLS86_07780"
     CDS             <1..1222
                     /locus_tag="VLS86_07780"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008573397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HSO50432.1"
                     /translation="ARFAVIKIGGAILRDSLDETAAALAFLHTVGLTPIVIHGGGPQL
                     DETLKSRGIGNQRVDGLRVTGPDVLDAARDTFIEQNILLVEAVRNQGVAAHGLTEGAF
                     EADYIDRAKYGLVGEPTEVHLPLIRSIVASSAIPILTCLGVAPGGQLVNMNADSATRA
                     LVHAVQPMKIIFLAGVGGLLDAEGRLIESINLASDYDHLMSQEWVHSGMRLKLAEIKL
                     LLDDTPLSTSVSITTPSGLVKELFTHSGSGTMVRRGELIEKELDKAALDRGQVEKLVE
                     EAFGRTLDPRWWDGLGLETAYLSESLRAGALIARIDDFAYLDKFAILEDARGEGLART
                     VWAHVVADYPVLYWRSRTENGFNAFYAEEADGSVKRGPWTVFWKGEPDFSRIAARVEA
                     VASLPASFIGDHDE"
     gene            1215..>1631
                     /locus_tag="VLS86_07785"
     CDS             1215..>1631
                     /locus_tag="VLS86_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HSO50433.1"
                     /translation="MSDRFTVGLVGARGHTGAEIVRLVAGHPSLDLVYAGSRELAGQP
                     VPGVDLKLESITGDEMAEQTADVVVLALPDGASDPYVETIGGDTVIVDISADHRFDPY
                     WAYGLPELQRDRLQGATRIANPGCYATAMQLALAPVV"
BASE COUNT          283 a          539 c          521 g          288 t
ORIGIN      
        1 agcccggttt gcggtgatca agatcggcgg cgccattctc cgtgactcgc tggacgagac
       61 ggcagccgcc ctcgccttcc tccacaccgt cggtctcacc ccgatcgtca ttcacggtgg
      121 tggcccccaa ctcgacgaga cgctgaagag ccgaggcatt ggcaatcaac gggtcgatgg
      181 cctccgggtg acgggccctg acgtgctcga cgcagcacgg gacacgttca tcgagcagaa
      241 catcctgctg gtggaagcgg ttaggaacca gggggttgcc gcacacgggc tcaccgaggg
      301 tgctttcgag gccgactaca tcgaccgggc caagtacggg ctggtcggtg agccaaccga
      361 agtccatctg ccactgatcc gctcgatcgt ggcttcgagt gcgatcccga tcctcacctg
      421 tcttggggtc gcccccggcg gtcagctggt gaacatgaac gccgactcgg caacccgcgc
      481 cctggttcat gcagtgcagc cgatgaagat catcttcctg gccggggtcg gcgggctcct
      541 cgatgctgaa ggcagactga tcgaatcgat caacctcgcc tccgactatg accatctgat
      601 gagtcaggaa tgggtgcatt cggggatgag gctgaagctg gccgagatca agctcctcct
      661 cgacgacacc ccactgtcca cctcggtctc gatcaccaca ccttccgggc tggtcaagga
      721 gctgttcacc cacagcgggt cggggacgat ggtgcgccgc ggagagctga tcgaaaagga
      781 gctcgacaag gccgctctcg accggggtca ggtggagaag ctggtcgagg aggcgttcgg
      841 aaggaccctg gacccccgat ggtgggacgg tctcggtctg gagactgcct acctgtccga
      901 gagtctccga gccggggcct tgatcgcgcg catcgacgac ttcgcctatc tcgacaagtt
      961 cgccatcctc gaggacgctc gcggtgaagg gttggcgaga accgtgtggg cccatgtcgt
     1021 cgccgactat ccggttctct attggcgctc ccggaccgag aacgggttca acgccttcta
     1081 cgcagaggaa gccgacggat cggtgaagcg tggcccgtgg accgtgttct ggaagggcga
     1141 gcccgacttc agccgcatcg cggcccgggt ggaggctgtc gcctcactcc ccgcctcctt
     1201 catcggcgac cacgatgagt gatcgattca cggttggcct ggtcggggcc cgcggccaca
     1261 ccggcgccga gatcgtccga ctcgtcgccg gccacccatc gctcgatctg gtctacgccg
     1321 gttcccgtga gctggccgga caacctgtcc ccggtgtcga tctgaagctc gaatcgatca
     1381 ccggcgacga gatggccgag caaacagccg acgtggtggt cctggccctg cccgacgggg
     1441 ccagtgaccc gtacgtcgag acgatcggcg gtgacaccgt catcgtcgac atctccgccg
     1501 accatcgatt cgacccctac tgggcctatg ggctccccga gctgcagaga gatcggctcc
     1561 agggcgcaac ccgcatcgcc aaccccggct gttacgccac cgcgatgcag ctggctctgg
     1621 ccccggttgt c
//
LOCUS       DATITW010000559         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_187731, whole genome shotgun sequence.
ACCESSION   DATITW010000559 DATITW010000000
VERSION     DATITW010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187731"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VLS86_07790"
     CDS             complement(<1..642)
                     /locus_tag="VLS86_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50434.1"
                     /translation="MVSDEDTARSAGPMVGRSIPAPAVLFFTDNGVGLGHLTRLIAVA
                     QQARGRFQPVFLTMSLGYQLLRDGGIPSEFFPAYHQLGLTKAEWIPLLTMRLSEAIEQ
                     SKARVVVVDHVLPPASFQVLHAGLPAVEFVWSRRGLWREGKNEGAFEVAKGFDVVIEP
                     GDLAAPIDMGASATRRAGVLPITPVVLVQPGEFLSRDEARTALGIPKEGRAILL"
     gene            complement(693..1298)
                     /locus_tag="VLS86_07795"
     CDS             complement(693..1298)
                     /locus_tag="VLS86_07795"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSO50435.1"
                     /translation="MSSAFLYRIVQHTLAPRSSRVAFVHDYVRPGPEARILDVGCGPA
                     DDLTYMPDTVGYVGFDLSERYVEAARSRWGERGTFFQAEVSPSLLQGDEFDIVIANGV
                     LHHLNDGEALGLLNLAHSVLRPGGRLVTKDPVFVEDQHPVARFLVKRDRGGFVRDTSG
                     YLGLAHQVFAETTLVTRSDMLRCPYDHAIMEMSRPGGSTPT"
     gene            complement(1425..>2210)
                     /locus_tag="VLS86_07800"
     CDS             complement(1425..>2210)
                     /locus_tag="VLS86_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50436.1"
                     /translation="NQEFERGLYTHVAEYWDVLIGHSFHYATYGAPPRWATSILAVLV
                     IGLAVLGLVGPATRRSDLERTSSDGIRIGALPLVWAIGVVLTSFFMFFIGTVRDRYLL
                     MGLVFIATALIAGAWRLFESSRFDRRLVVGALAGLALVWAVSNVAIAGVYEKGRFESG
                     SETEYFARTLHRLAAGRDCLGVSRFGAPQIQFGSGCVTETATSADDAAAWAAGARAPG
                     RLVFVSWPRGDAGSLDLAPEEWTEIPRSPDGEDNVVVLWSDGS"
BASE COUNT          444 a          737 c          686 g          343 t
ORIGIN      
        1 gaggagtatc gcccggcctt ccttggggat gccgagggcg gtgcgagctt cgtcccgtga
       61 caggaactcc ccgggctgga cgagcacgac aggtgtgatc ggtagcacgc cggcgcgtcg
      121 ggtcgccgag gcgcccatat cgatgggcgc ggctaggtcc cctggttcga tgacgacgtc
      181 gaatcccttc gccacctcga acgccccctc gttctttccc tcccgccaca ggccacgcct
      241 cgaccacacg aactccacag cggggaggcc ggcgtgcagc acctgaaaac tcgccggggg
      301 aaggacatga tcgacgacga ccactcgggc tttcgactgt tcgatcgcct cagacagacg
      361 catggtgagc aggggaatcc actcggcctt ggtcagcccc aactggtggt aggcggggaa
      421 gaactctgac ggtatcccgc cgtctctgag cagctgatag cccagcgaca tggtgaggaa
      481 caccggctgg aagcgccctc gcgcttgttg ggccacggcg atgagacggg tcagatgtcc
      541 gagacccacg ccattgtcgg tgaagaacag gaccgctgga gccggaatag accgacccac
      601 catcggcccc gccgagcgcg ccgtgtcctc atcactgacc atctgaaacg tcaatctcgc
      661 aactgcctct cgagctgttt ggcgagcggc ggctaggtcg gtgtcgaccc accgggccgg
      721 ctcatctcca tgatcgcatg gtcgtaggga caacgaagca tgtcgctgcg ggtgaccaga
      781 gtcgtttctg cgaatacctg gtgggcgagg ccaaggtaac cagaggtgtc gcggacaaag
      841 ccgccccggt cacgcttcac cagaaagcgg gccacggggt gctggtcttc gacgaatacc
      901 gggtccttgg tgaccagacg cccacctggt cggaggacgg agtgagccag attcaacaga
      961 cccagggcct ccccgtcatt cagatgatgg agcaccccgt tggcgatgac gatgtcgaac
     1021 tcgtcgccct gcaacagtga cggcgagacc tcggcctgga agaacgtccc tctctccccc
     1081 cagcgactcc gggcggcctc cacgtagcgt tcgctcagat cgaagccgac atagccgacc
     1141 gtgtcaggca tgtaggtcag atcgtcggcc ggaccacatc ccacgtccag gatccgagcc
     1201 tcgggcccgg gacggacgta gtcgtggacg aaggcgacac gactggaacg aggggccagc
     1261 gtgtgctgca cgattcgata gagaaacgcc gagctcaaca gtcgccgagg atctgctgcg
     1321 tgcaaccacc cggtcgtttt ctccgacgta ttgctcattc tcgggtcaag aatacgccca
     1381 gtccgtgccg agccctggaa acgagtgagt cacgcgtcgg ccgtctatga cccgtccgac
     1441 cacaggacaa cgacattgtc ctcgccatca ggggatcgag ggatctcggt ccactcctcc
     1501 ggtgccaggt cgagtgagcc ggcgtctccc ctcggccacg agacgaagac gagacgcccc
     1561 ggcgccctgg ccccggcagc ccacgctgcc gcgtcatctg ccgaagtcgc ggtctcggtg
     1621 acgcatccgc ttccgaattg gatctgcggg gcaccgaatc gcgagacccc gaggcaatct
     1681 cgacccgccg ccaggcgatg gagggtccgt gcgaaatact cggtctccga gccactctcg
     1741 aaccgaccct tctcgtacac gccggcgatc gcgacattcg acaccgccca gaccagtgcg
     1801 agaccggcaa gagccccaac caccagacgt cggtcgaatc ggctcgactc gaacagacgc
     1861 caggcccctg cgatcagcgc ggttgcgatg aacaccaagc ccatcaagag atagcggtca
     1921 cgcaccgtgc cgatgaagaa catgaagaac gacgtgagca ccaccccgat tgcccacacc
     1981 aggggcaagg cgccgattcg aatcccgtcg gatgacgtcc gctcgagatc ggaacgccgg
     2041 gtcgccggtc cgacgagccc gagaacggcc aacccgatca ccagcaccgc gagaatcgac
     2101 gtggcccagc gcggtggggc cccatatgtg gcgtagtgaa acgagtggcc gatcagaacg
     2161 tcccagtatt cggcgacgtg ggtgtacaag ccgcgctcga actcctggtt
//
LOCUS       DATITW010000560         1539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_468385, whole genome shotgun sequence.
ACCESSION   DATITW010000560 DATITW010000000
VERSION     DATITW010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468385"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VLS86_07805"
     CDS             <1..552
                     /locus_tag="VLS86_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO50437.1"
                     /translation="MVDPLETDLLLYDQMLEYAADIGDEELLQLMEGYGRPPYADLYG
                     YMTVLEHYGDLEPYTETAEFAAGVPGIQGTGVSEYGFMDKLNVFRGLADMGGHLYPQA
                     QDIDFRRDVPKLEVPVYLIQGGHELRARASLAEEYASVLEAPSLDVVVFENSGHIPHF
                     EEANRFHRYLVDVVLAETDGAQS"
     gene            complement(543..1352)
                     /locus_tag="VLS86_07810"
     CDS             complement(543..1352)
                     /locus_tag="VLS86_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSO50438.1"
                     /translation="MATLDAKKRASLPNSAFAYVDSKGNRRLPINDEAHVRNALSRFN
                     QVKFESEEARERAFRRLLSAASSYGIAPVGFVTRQLREARAGSTKSMPSGQVTLLLSD
                     IEGSTPLVHSLGADYPALLEEVRSTIRESVGGHHGYEVDSRADEFFAAFADAVDAVQA
                     AIEIQRRLESSPAKVRIGLHSGSPALTDTGYVGLPVHVMARVCNAGHGGQILITAATR
                     DELEGAMPEGIGLVSLGAYRLQGLPDAEELFQVSARGLSASFPELRVPDQD"
BASE COUNT          280 a          496 c          495 g          268 t
ORIGIN      
        1 atggtcgacc cgctggagac cgaccttctc ctctatgacc agatgctcga gtacgcagca
       61 gacatcgggg acgaggagct gttgcagctc atggaagggt acgggagacc gccctacgcc
      121 gacctctacg gctacatgac ggtcctcgag cattacggcg atctcgagcc ctacaccgag
      181 actgccgagt ttgcagccgg cgtccccgga atccagggga ctggggtcag cgagtacggg
      241 ttcatggaca agctcaatgt gttccgcggg ctggcagaca tgggcgggca cctctaccca
      301 caggcgcagg acatcgactt cagacgcgac gtgcccaagc tcgaggttcc cgtctatctg
      361 atccagggcg gccacgagct gagggcaagg gcttcactcg ccgaggaata cgcgtccgtg
      421 ctcgaggcgc cgtcgctcga cgtggtcgtg ttcgagaact cgggccacat cccccacttc
      481 gaggaggcaa accggttcca tcggtatctg gtcgacgtgg tgctggccga gaccgacggg
      541 gctcagtcct gatcggggac cctgagctcg gggaatgatg cgctgagccc gcgagcactc
      601 acctggaaca gctcttcggc gtcgggcagc ccttgaagcc gatacgcccc gagactcacc
      661 agcccgatcc cctcgggcat cgcgccctcc aactcgtcgc gggtggctgc cgtgatcagg
      721 atctgcccac cgtgccccgc gttgcagacc cgggccatga cgtggaccgg gagcccgaca
      781 tagccggtgt cggtgagagc cgggcttccg ctgtgtagtc cgatcctcac cttcgccggg
      841 gacgactcga gtcgtcgttg gatctcgatg gcggcttgga ccgcgtccac tgcgtcggcg
      901 aatgcggcaa agaactcatc ggcgcgggaa tcgacctcgt atccgtggtg acctccaacg
      961 ctctccctga tcgtggaccg aacctcctcg agcagagccg gataatcggc gcccagcgag
     1021 tgaaccagcg gagttgaccc ctcgatgtcg ctgaggagaa gcgtgacctg gcccgacggc
     1081 atcgatttgg tcgagcccgc tcgagcctcg cgcaattgtc gcgtgacaaa gcccaccggc
     1141 gcgatcccgt agctggaggc ggcagacagc agcctgcgaa atgccctttc tcgcgcctcc
     1201 tcgctctcga acttgacctg gttgaaacgg gagagggcgt tgcgaacatg ggcctcgtcg
     1261 ttgatcggga gccgccggtt gcccttggag tcgacgtagg cgaaggcgct attggggaga
     1321 ctcgcccgct tcttggcgtc gagggtggcc atatatcact ggacggtagg cgcattgtgc
     1381 ggtttcgcga cctgacgcat tggtgccgca ctcatcagga cccgctggat caaccacagt
     1441 ccatctgacc tgatcgcggc gaccacgcac atgatcggag gacaatccgg cccgactgag
     1501 cccgatcaac ctgccgggat cggcgccgag aagaggacc
//
LOCUS       DATITW010000561         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_28135, whole genome shotgun sequence.
ACCESSION   DATITW010000561 DATITW010000000
VERSION     DATITW010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28135"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VLS86_07815"
     CDS             complement(<1..332)
                     /locus_tag="VLS86_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50439.1"
                     /translation="MSTPHAIETRASRVRWIMAGLVALLVFGSVPAVGLSRFTSADED
                     LFKETKHELKQSKHELHKAKDKYRAQTRKIRALQRVMNRLATRISRAEGEILRAETRL
                     DKLQRQMM"
     gene            1042..2244
                     /locus_tag="VLS86_07820"
     CDS             1042..2244
                     /locus_tag="VLS86_07820"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003942744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HSO50440.1"
                     /translation="MSKRRKDVFVMIEEFGGGVGVRSWASILDDKTREQAETTALLPI
                     LTEPIALMADAHLGYGATIGSVIPTESAVIPSAVGVDIGCGMAARKVDLAGMRALDHA
                     ERWVHDCEASIPAGMGKGRSGFSAEAKDWMGAHLPPATLENPQRVEAQLGSLGSGNHF
                     IELAVDQDDGTWILLHSGSRGPGNQLATLHTKVAMDLHERLGTALDDPELSWLQSNTP
                     EFNAYIHDLRWAQAFALENRRLLLDAAHDQLNEASGEGVRTLDEVNCHHNYTELEEHD
                     GKWLWVTRKGAIRAGVGDRGLIPGAMGQKSFVVRGLGNPMSYESCSHGAGRVLSRTKA
                     RKTLSVEDLESQMSGRSWQASDAKSLLDEAPQAYKPIDVVMRDQADLVEIEVALSSIA
                     NYKGVERR"
BASE COUNT          517 a          876 c          991 g          580 t
ORIGIN      
        1 ttcatcatct gccgctggag cttgtcgaga cgggtctcgg cccgcaggat ctctccctcg
       61 gctcgcgaga tgcgcgtggc caaacggttc atcaccctct gcagggcgcg gatcttccgc
      121 gtctgtgcgc ggtacttgtc cttggccttg tgcagctcgt gcttgctctg cttcagctcg
      181 tgcttggtct ccttgaacag gtcctcgtcg gccgaggtga acctggagag accgaccgcg
      241 ggcacggatc caaacacgag aagcgcgacc aatccggcca tgatccagcg cacgcgcgat
      301 gcgcgtgtct cgatggcgtg cggggtcgac atctcggcag gttcatcgct gccgttcgtc
      361 ctgggacgtt cttcgttttc ggtcatgtca ccggaccatc ggcagaccga gacgttgctt
      421 gaggaaccac gatgaaacct gtctgggcgt cggcgcgtcg catagcgacg gtcgtttggg
      481 taacccaggc acgcgcgcga ctttgtcgct ccaagcgggg gtccacgtga gcatcgtgtc
      541 cgagcggctc aggcacagct cggtgagaat tgtgctcgac acctagccac gcgatcccag
      601 gcctgcagcg ggacgccccc gagcaagtgg gtctcctggc cgattgccgg tagagcgaca
      661 aagagaggat cctcaggtca gaggtagtgc gagaggcgag agtcgaactc ccacgccctt
      721 tcgggcaccg gatcctaagt ctgctgccgc caggcacgga ttccggcacc acgtgtcgtc
      781 ctgtgtcgtc tggtgtcgtc ctgtgccagc cggtgtcgtt ccgtcgtgag cgagatgtga
      841 gcaaggggag tcgcaccgca atgatccctg agcgatgaac cgccccgcga ttcttggagg
      901 ctcttcgctc agccatgagc aagaagttca cttcgaacgc atcgcaacga cccgcgccat
      961 aggcgtgcgt cgcttcttgc cgtcgtgcgc ctacccctcc acggaggggt tcttcgtttc
     1021 ccggagattc cgatcgaggg cgtgtccaag cgacggaagg acgtgttcgt gatgatcgaa
     1081 gagttcggtg gcggtgtcgg ggtgagatcg tgggcgtcga tcctcgacga caagacgaga
     1141 gagcaggcgg agacgaccgc gctccttcct atcctcacgg agccgatcgc gctgatggcc
     1201 gacgctcatc tcgggtacgg agcgacgatc ggttcggtga ttccgaccga atcagccgtg
     1261 atcccctcag cggtcggtgt ggatatcggg tgcggcatgg cggccagaaa ggtcgatctt
     1321 gccgggatga gggccctcga tcacgccgag aggtgggtgc acgattgcga agcctcgatc
     1381 cccgctggga tggggaaggg tcggtccggg ttctcggctg aggcaaagga ctggatggga
     1441 gcccaccttc ctccggcgac gctcgagaac ccgcagcggg tggaggcgca gctgggatcg
     1501 ctcggatcgg gcaaccactt catcgagctg gccgtcgatc aggacgacgg gacgtggatc
     1561 cttctgcatt ccggatccag gggaccgggc aaccagctgg cgacgcttca caccaaggtc
     1621 gcgatggacc tgcacgagcg gctcggcacc gcgctcgacg atccggagtt gtcctggctg
     1681 cagagcaaca cgccagaatt caacgcctac atccatgacc tgcggtgggc tcaggcgttc
     1741 gcgctggaga accgccggct cctgcttgac gccgcccacg atcagctgaa cgaggcgagc
     1801 ggtgaaggtg ttcggacgct ggacgaggtc aactgccatc acaactacac ggaactcgag
     1861 gaacacgatg gcaagtggct gtgggtgacg aggaagggtg ccatcagggc cggggtggga
     1921 gaccgggggc tcatccccgg cgcgatgggg cagaagtcat tcgtcgttcg gggcctgggg
     1981 aacccgatgt cgtacgaatc gtgttcccac ggcgcgggcc gggttctctc gaggacgaag
     2041 gccagaaaga cgctatccgt cgaggacctc gagtcgcaga tgtcggggag gtcgtggcag
     2101 gcgtcggatg ccaagtcgct tctggatgag gccccacagg cttacaagcc gatcgatgtg
     2161 gtgatgcggg accaggctga tctggtggag atcgaggtcg cgctgagttc gatcgccaac
     2221 tacaagggcg tcgaaaggag gtgaggatgg ccggtcggcg gagtcaggtg tcggtgttac
     2281 gcatgacgtc tatgctgcct gccgtacgct cgggtcggga tctgccgcaa gctccagcac
     2341 tttggaggta gccgcttccg cgtcgaagtt gtcgagttca tcgatcgcgt tcttgtcgaa
     2401 cgaccggatc cggatccttg agtgcttcga tcagcgttgg gatcgatcca cacccatgga
     2461 cgcgcgcccg gtatcggagg acagcccctc gcacgggtgg ccgttcatcg tcctgccggc
     2521 gcaagacctc ctcggcctta tcctcagttg tccagagtcg cgatgtcgat cctacggctc
     2581 cggtcagtct agaagggctt gttcgccttg atagcccagg cccatcgcgt cgcagcatcc
     2641 ggcgtgccgt ctggtcgttg caacgtgtac aggtactcga tcctcgcaaa gtcaatcgag
     2701 aaggtgtcag ttggagtcgt ttcgggatcc cccgtaccgc tctggtagct agagatcgtg
     2761 atgtccgaga gcctaagcac gatgaagtcg atggagaagt tgggacccgc ttctcgtcgc
     2821 gccgcaatag ctcagtttgc acgtgctcgc ccgtgacaca ggccttcgca agtctgggtg
     2881 aggccttgtc caccttcttt caaccggacg tcgttccgtt tggtgggcgg gcgatggagc
     2941 cggcgattcg gctgccatac ctgt
//
LOCUS       DATITW010000562         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_668497, whole genome shotgun sequence.
ACCESSION   DATITW010000562 DATITW010000000
VERSION     DATITW010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668497"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2017)
                     /locus_tag="VLS86_07825"
     CDS             complement(<1..2017)
                     /locus_tag="VLS86_07825"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSO50441.1"
                     /translation="MSLVGAPIRRREDPDLLTGRKRFVADLTAGCQAVAFVRSPMAAA
                     RIIGMELPEGASVFTAADLTGVGAIDPILHRPDYVRAPQPLLAVDRVNYLGEPIAAVV
                     AGSRGEAEDVAGAVYVDFEPQEPVVTAESALADNSRIVHPGAESNVVVSGAVDTGDLD
                     VTSESPVIRIEMEITCRRQSAAPIETRGAVARYDEETGKVTLYASTQMPHVLRTAICD
                     LLGMQEADLRVIAPAVGGAFGQKMCLPAEYAVLVWLAGHLRTALAWIEDRRENFTSSF
                     HSRDHRYQIQGSFDANARLVALEGDLLVNVGAYSCYPVTWGVEPLMAMAELPGPYDFQ
                     SYRVRARGVSTNTCPMSPYRGVSRPVLTLAMERMMDLAAERFGLDPVDIRRRNLITEF
                     PYRSATGIVYDEGSYLASLEKAAEVVDLDSFRRRQAEAREAGRFLGIGFSVFSERTGY
                     GTPAFAPRNMDVTPGYETVEMAMDPSGGVEVRIGASPHGQGLVTSLSQVVADELGLPF
                     ESIRVVHGDTDRTPYGWGTFASRSMVISGGATKMAAEKLREAIVAVAGDMLEAAPEDI
                     TLAGGRAVVRGTDRGVEMADLARHAYHQTPKLAPGREPGLRSTSTYDPAGTFSNACHV
                     AVVEVDIHTGGVRVDRFVVVEDAGLLINPVIVDGQISGGVVQGIANAL"
BASE COUNT          369 a          721 c          662 g          373 t
ORIGIN      
        1 acagggcgtt ggcgatgccc tgcaccactc cccctgagat ctggccgtcc acgatgacgg
       61 ggttgatgag aagtccggcg tcctcgacaa cgacgaatcg atcgacacga acgcctccgg
      121 tgtggatgtc cacctccacc accgcaacgt ggcatgcgtt ggagaacgtc ccagccgggt
      181 cgtaggtgga ggtcgaacgg agacctggct ctcgtcccgg ggcgagcttc ggggtctggt
      241 gataggcgtg ccgggcgaga tcagccattt ccacaccccg atcggtcccc cgtaccaccg
      301 cccgccctcc ggcgagggtg atgtcttcgg gcgctgcctc cagcatgtcc ccggcaacag
      361 ccacgatcgc ctcgcgcagc ttctccgcgg ccattttggt ggcgccgcca ctgatcacca
      421 tcgagcggct ggcgaaggtg ccccacccat agggagtgcg gtcggtgtcg ccatggacga
      481 ctcggatcga ctcgaaaggc agaccgagct catcggcgac cacctggctc agcgatgtca
      541 ccaacccctg gccgtgcggg ctggctccga tccttacttc cacccctccc gaagggtcca
      601 ttgccatctc caccgtctcg tacccgggag tcacgtccat gttcctcggg gcgaatgccg
      661 gggtgccgta tccggtgcgt tcggagaaca ccgagaaacc gatcccgaga aacctcccgg
      721 cctccctggc ctcggcctgc cggcgccgga acgaatcgag gtccacgacc tctgccgcct
      781 tctccagcga agcgaggtag gagccttcgt cgtacacgat tccggtcgcc gatcgatacg
      841 gaaactcggt gatcaggttg cggcgtctga tgtcaaccgg gtccaatccg aatcgctccg
      901 cggccaggtc catcattcgc tccatggcca gggtgagaac cggtctcgag actccgcggt
      961 atggcgacat cgggcaggtg ttggtggaca cgcccctggc gcgcacccgg tatgactgga
     1021 agtcgtatgg cccggggagc tcggccatcg ccatcagagg ttccacaccc caggtgaccg
     1081 gatagcagga gtaggcgccc acattgacca gcagatctcc ttctagagca acgagccggg
     1141 cattggcatc gaagctccct tggatctgat agcgatggtc ccggctatgg aaagatgagg
     1201 tgaagttctc ccgtcggtct tcgatccacg ccagcgctgt gcgcagatgt cctgccagcc
     1261 acaccagtac cgcgtattcg gcagggagac acatcttctg gccgaaggcg ccacccactg
     1321 ccggggcgat cacccggagg tctgcttcct gcattccgag gaggtcgcag atcgccgtgc
     1381 gcaggacatg cggcatctgc gtcgaggcgt acagggtgac tttccccgtc tcctcgtcgt
     1441 aacgggccac cgctcccctg gtctcgatcg gagctgcgct ctgtcttcgg caggtgatct
     1501 ccatctcgat gcgaattacc ggagactcag aggtgacgtc gagatcgcca gtgtcgaccg
     1561 cgcccgaaac gaccacgttg gactcggcac cgggatgaac gatccggctg ttgtctgcca
     1621 gagcggattc ggcggtcacc accggctcct gcggctcgaa gtcgacgtag accgcaccgg
     1681 ccacgtcttc ggcctccccg cggctaccgg ccaccaccgc tgcaattggc tctccgaggt
     1741 agttgactcg gtccacggcc aacagcggct ggggagcccg gacgtagtcc ggacgatgga
     1801 ggatggggtc gatggccccc actccggtca gatccgcggc tgtgaacacc gaggcccctt
     1861 cggggagctc catcccgatg atgcgggccg cggccatcgg cgagcgcaca aaggccacgg
     1921 cttggcagcc ggcggtcaga tcggccacga atcgtttgcg accggtcagg agatcgggat
     1981 cctcccgccg ccggataggg gcaccgacca ggctcatccg gtcgcctgtt ggcaggctct
     2041 ctgaatcaag gccccaagca ggtcgcgtcg atattcggcc gagccatgga tgtcctcgaa
     2101 aggctccacc tctgcccgtc ccgct
//
LOCUS       DATITW010000563         1984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_788660, whole genome shotgun sequence.
ACCESSION   DATITW010000563 DATITW010000000
VERSION     DATITW010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788660"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VLS86_07830"
     CDS             complement(<1..232)
                     /locus_tag="VLS86_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50442.1"
                     /translation="MTVSVWSMFAAIWIGLGVVLITSPESIEEMWAWIGGLPTLIQVA
                     IWIVFLPVMIAVAVLQTDWEPWVRIGVFVLCVT"
     gene            complement(460..1194)
                     /locus_tag="VLS86_07835"
     CDS             complement(460..1194)
                     /locus_tag="VLS86_07835"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HSO50443.1"
                     /translation="MPDSFQRALVREGRPKLDVPAARAQHHRYRQHLEESGYSIEVVP
                     PDEDQPDCVFIEDTAVVIRQVALITRPGAPSRRDETGPVAEALRTRFPVVEVVAPGTI
                     DGGDVFLTPEVVYVGRSSRTNEAGIDQLRSLALDLGMSLVVVEVHDGLHLKSAVLPID
                     PETVVVTPHSVDEMALNGLRIVYEVDEERNRFSALPLRDGRILVTADAPATAGAVSAL
                     GHEVVPIDVSQIQAADGGLTCMSILL"
     gene            complement(1247..>1984)
                     /locus_tag="VLS86_07840"
     CDS             complement(1247..>1984)
                     /locus_tag="VLS86_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HSO50444.1"
                     /translation="NRGMTRSLLDYDALSFDCYGTIINWESGIWAALQPLLVANDATL
                     LRSEALSVFGELESDQEASTPELAYPLILARVHASLAGRYGLATTEDLDRRFGSSVPE
                     WPAFGDSAEALSSLGERYRLVILSNVDRSGFAASNEKLGVEFDAIYTAEDIGSYKPDP
                     ANFDYLTGRVEADLGVTSEALLHVAQSLFHDHAPAKAAGLETVWIDRQRLSEGGDWGA
                     TRRLEERPEPDHVFFSLAELAAAVEMT"
BASE COUNT          363 a          660 c          643 g          318 t
ORIGIN      
        1 aggtcacgca gaggacaaag accccgatac gcacccacgg ctcccagtcc gtctgcagca
       61 cggcgaccgc gatcatcacc gggaggaaca cgatccatat cgcgacctgg atcagtgtcg
      121 gcagcccccc gatccatgcc cacatctctt cgatcgactc cggcgatgtg atcaggacga
      181 ctccgagacc tatccagatc gcggcgaaca tcgaccacac ggaaacagtc acccatcgtg
      241 gctgatgcca aacactgccc tttggcatgt tggccggggc ccgcgggacg agcccaggcg
      301 gcaggtgtac tccctgaatc atttgcgatc ctttcctacc cgatactgcc tgtccgggtc
      361 agggctcggg tagaggacaa cgacccgatc cgtccagggc tgtcgccgca atcggtggcg
      421 aggtggaccc tggtggcggc gtcgatgcag ccgggtctct cagaggagaa tcgacataca
      481 ggtcagcccg ccgtccgccg cctggatctg tgagacgtcg atcggaacca cctcgtgacc
      541 cagcgccgaa accgcaccgg ccgttgccgg ggcgtcggcg gtgacgagga tccggccgtc
      601 ccggagcggc aaagcgctga accggtttcg ctcctcgtcc acctcgtaga cgatacggag
      661 accgttgagt gccatctcgt ccaccgagtg tggggtcacc accacggtct cggggtcgat
      721 ggggagcacg gccgacttca gatggaggcc gtcgtgaacc tcgaccacca ccagactcat
      781 cccgaggtcc agggccaacg accgaagctg gtcgatgccg gcttcgttgg tccgggacga
      841 cctccccaca tagaccacct cgggagtgag gaacacgtca ccaccgtcga tcgtccccgg
      901 tgccacgact tccacgaccg ggaaccgcgt tcgcagcgcc tctgccaccg ggccggtctc
      961 gtcgcgacgg ctcggggccc cgggacgggt gatgagcgcc acctggcgga tcacaaccgc
     1021 ggtgtcctcg atgaagacgc aatcgggctg atcctcgtcg ggagggacca cttcgatcga
     1081 gtacccgctc tcctcgaggt gttgtcggta acggtggtgc tgggcacggg ccgccgggac
     1141 atcgagcttg ggccgtccct cacgaaccag cgctcgctgg aagctgtcgg gcaccggtcg
     1201 aatcaatgca gtaggcgcca ccatcgcggc atcgtacgag aggacctcag gtcatttcga
     1261 ctgctgcggc cagctcggcc agggagaaga agacatggtc cggctccggc cgctcctcga
     1321 gacgtctcgt cgccccccag tctccaccct cggagagacg ctgccgatcg atccagaccg
     1381 tctccagacc ggctgccttg gccggggcgt ggtcgtggaa caggctctgg gccacgtgaa
     1441 ggagggcctc ggaggtgacg ccgaggtcgg cctcgacccg gcccgtcaga tagtcgaagt
     1501 tcgccgggtc cggcttgtag gagccgatgt cctcggcggt gtagatcgca tcgaactcca
     1561 cgcccagctt ctcattggag gcagcgaagc cggagcggtc gacattggac aggatcacga
     1621 gacgatatcg ctcgcccaga gacgagagag cctccgcaga gtccccgaac gccggccact
     1681 cgggcaccga ggagccgaat cgccggtcga ggtcttcggt ggtggccagg ccgtatctcc
     1741 cggcgagtga tgcgtggacc cgggccagga tcaggggata ggccagctcc ggtgtcgagg
     1801 cctcctggtc ggactccaac tcaccgaaca cgctgagcgc ctccgatcgg agcagggtcg
     1861 catcgttggc gacgagcagc ggctgcaggg ccgcccagat gccgctctcc cagttgatga
     1921 tggtcccgta acagtcgaag gagagagcgt cgtagtcgag gaggcttcgt gtcatgcccc
     1981 gatt
//
LOCUS       DATITW010000564         1532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_68499, whole genome shotgun sequence.
ACCESSION   DATITW010000564 DATITW010000000
VERSION     DATITW010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68499"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..904)
                     /locus_tag="VLS86_07845"
     CDS             complement(203..904)
                     /locus_tag="VLS86_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50445.1"
                     /translation="MLTIHKLNAGYGPIKALWDVSISAAEAEIVAVLGANGAGKSTLV
                     KCVVGLLDPISGMIKLGGQRIEGSRPHRISELGIAYAPEGGGVFRDMIVRENLEMGAY
                     HQRAWERRDKTLERVFRLFPRLEEREHALARHLSGGERQMLVIGRALMSLPTLCVFDE
                     PSIGMSPLLVEQSFHVISQLRDEGITILLIEQNVRKTLEIADRAYVLQNGRVVMEGVA
                     SALQSDLVDKAYLGL"
     gene            complement(906..>1532)
                     /locus_tag="VLS86_07850"
     CDS             complement(906..>1532)
                     /locus_tag="VLS86_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO50446.1"
                     /translation="GLIGPNGAGKTTVFNLISGAIRARQGSIRFGGEEITGLRPDQIS
                     RRGLARTFQTTTLFTDMSAFENVRLALIYGNPERRFRNEEAEREVNRLMASMGILADR
                     NKSIREMSLASRRYLEITRAIATNAEMLLLDEAMAGLTPSELEQSKRLVSRLRDQGIT
                     ILMIEHVMEAVMEVCDRIIVLHFGKKVAEGSPVEVATNSTVRNIYLGE"
BASE COUNT          260 a          494 c          476 g          302 t
ORIGIN      
        1 atcagggcga ggatcgccat cagcgacagc gccgactgca agccgtcgag gcgcgctccc
       61 acgtggcatc gacggtctca gctggcaggc ctgaatcctc gagagcggtt cgaaggccgg
      121 cgtcggagac aaaggggatg ccggcggcca gctccacctc cgcttgggcg tgctcaccgt
      181 catcgatcga atacggagcg ggtcagagcc cgaggtatgc cttgtcgacg agatccgact
      241 gcaatgcaga ggcgactccc tccatcacca ctcgcccgtt ctgcagtaca taggctcggt
      301 cggcgatttc cagggttttc ctcacgttct gctcgatgag gaggatggtg atgccctcgt
      361 cacgcagctg agagatcaca tggaaggact gttccaccag tagtggggac atcccgatcg
      421 acggctcgtc gaaaacgcag agggtgggga gtgacatcag tgcacggccg atcaccagca
      481 tctgccgttc ccctccactg aggtgcctgg ccagagcgtg ctccctctcc tccagccggg
      541 ggaacagccg aaagactctc tccagggtct tgtcccgtcg ctcccatgcc cgctggtggt
      601 aggcgcccat ctccaggttc tcccggacga tcatgtccct gaacacaccg cctccttcgg
      661 gggcgtaggc gattccgagc tcgctgatcc tgtggggtcg gctgccctcg atccgctggc
      721 cgccgagctt gatcattccc gagatgggat cgagcaggcc cacgacgcac ttgacgagtg
      781 tgctcttgcc ggcgccgttc gcccccagca ccgcgacgat ctcggcctcg gctgcggaga
      841 tggaaacgtc ccacagcgcc ttgatcgggc cgtagccggc gttgagcttg tggatagtga
      901 gcaagctatt cccccaggta gatgttgcgc acggtcgagt tcgtggccac ctcgaccggt
      961 gatccctcgg cgaccttctt gccgaagtgg aggacgatga ttcggtcaca cacctccatc
     1021 accgcctcca tgacgtgttc gatcatgagg atggtgatgc cttggtcacg gagtcgggac
     1081 accagccgct tggattgctc cagttcagat ggggtgaggc ctgccatcgc ttcgtcgagg
     1141 aggagcatct cggcattggt ggcgatcgcc cgggtgatct cgaggtagcg gcggcttgcc
     1201 agggacatct cccggatcga cttgttccga tcggcgagaa tccccatcga agccatgagc
     1261 cggttcacct ctctttcggc ctcctcgttg cgaaacctcc gctccgggtt gccgtagatc
     1321 agggcaagcc ggacgttctc gaaggcgctc atgtccgtga agagcgtcgt cgtctggaag
     1381 gtgcgcgcca gccctcgccg actgatctgg tcgggtcgaa gcccggtgat ctcttctcct
     1441 ccgaaccgga tcgagccctg tcttgcccgg atcgcacccg agatcagatt gaagaccgtg
     1501 gtcttccccg ccccgttggg gccgatgagc cc
//
LOCUS       DATITW010000565         1859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_788896, whole genome shotgun sequence.
ACCESSION   DATITW010000565 DATITW010000000
VERSION     DATITW010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788896"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VLS86_07855"
     CDS             <1..501
                     /locus_tag="VLS86_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSO50447.1"
                     /translation="LVSIGRTRIGHVSGPTNWHEALDRREGWRRGLERHHLAADSALC
                     EEGDWSPESGAAAMSALLDRIPDLDAVVAANDRMALGAMHALRARGLRIPDDVAVTGF
                     DDIEEAAWFAPPLTSVEQPLTEMGRQAVRRLRYEFGGSANPPVAIPLRAELMIRESTI
                     GTGRSA"
     gene            681..1241
                     /locus_tag="VLS86_07860"
     CDS             681..1241
                     /locus_tag="VLS86_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HSO50448.1"
                     /translation="MANLIYAISASLDGYIADENGNFDWTEISDDAHAFFNDLQRSVG
                     TFLFGRRMYETMRVWDTFVLDDLPEIQREFAEAWRATDKVVYSSTLDTVPEPRTRLDR
                     TFDPEVVRTMKDKADRDLGVGGAGLAAEAIRAGLVDRYLLRLLPTIVGGGTSALPDAV
                     RIDLALNTTRRFDDGSVVLDYRQRTA"
     gene            1533..>1859
                     /locus_tag="VLS86_07865"
     CDS             1533..>1859
                     /locus_tag="VLS86_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014690989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HSO50449.1"
                     /translation="MAEAQRDQPPAQVGDDSALQQFLLQLGSALTAAGEAVNDVQDHL
                     QAVAVAYGANEARFVVLPTFLVVALDPRRPATLEPTSQLSGVLRLDQTSGLFDILKKA
                     TKAEIDP"
BASE COUNT          348 a          592 c          580 g          339 t
ORIGIN      
        1 ctggtgtcga tcggcagaac ccggattggc catgtctccg gccccaccaa ctggcatgag
       61 gcgttggacc gccgagaggg atggcggcgc ggattggaga ggcatcacct cgcggctgac
      121 tccgctctgt gcgaagaggg cgactggtct cccgaaagtg gcgctgctgc catgagtgcc
      181 cttctcgacc gcatcccgga tctcgatgcc gtggtggccg ccaacgaccg catggcgctg
      241 ggagcgatgc acgcccttcg agccaggggg ctgcgaatcc ccgacgacgt ggcggtcacc
      301 ggctttgacg acatcgagga agccgcttgg tttgccccac cgctcacctc ggttgagcag
      361 ccactcaccg aaatggggag acaggcggtg cgccggctgc ggtatgaatt cggtggcagc
      421 gcgaaccccc ccgtcgccat ccccctccgc gccgagctga tgattcgcga gagcacgatc
      481 ggcaccggcc gctcggcgta gtcgcggcaa tcaataccgg acggtctcgt cacactgatc
      541 tcacgagtcg tgctcgacga gacggctggc ccgacatggc gctattgccc gcgttcgagg
      601 tgccggaaaa accatctacg gtctgatccg ttccagcctt gtgccgagca tgttgaatag
      661 ggaagagaat cggaggatcc atggccaacc tcatctacgc aatcagcgca tcactcgacg
      721 gctacatcgc cgacgagaac ggcaacttcg attggacaga gatatctgat gacgcgcatg
      781 cattcttcaa cgacctccaa cgatcggtcg gcaccttcct tttcggccgt cgcatgtatg
      841 agacgatgag ggtgtgggac acgtttgtcc tcgacgacct ccctgagata caacgcgagt
      901 tcgccgaggc gtggcgggcg accgacaagg tcgtctactc atccaccctg gacacggtgc
      961 cggaaccgcg gacccgactg gaccggacct tcgatcccga ggtggtacgg accatgaagg
     1021 acaaagccga ccgtgacctg ggcgtcggcg gcgccggcct cgccgccgag gccatccggg
     1081 caggtctcgt cgaccggtat ctacttcgtt tgctcccgac gatcgtgggc ggaggcacct
     1141 ctgcgctccc ggatgcagtg aggatcgacc tcgctctcaa cacgacacgt cgcttcgacg
     1201 acgggtccgt ggttctcgac taccgacagc gaacagcctg atgggcggag gtgtcacgtg
     1261 gaccggtcct ttggcgggca tgtggaacga ccggcgcgag acttcatgag aatgaccgga
     1321 cgaggtctcg aagcgtttcc tccaccttgg gggatgctca gatagcaagg cagagattca
     1381 gtcgagagga tgttgagtcg gcaatgtcct cggtgacgct ccacatcggg gacatggtgt
     1441 ctgggatcac tggcctaatc gctgacctga ggacccatct aggtgagtcc catcctcgtc
     1501 ttggacaccg gccggccaga ggttgattcc tggtggccga agcacagaga gatcagccgc
     1561 ccgcccaggt tggcgacgac tcggccttgc agcagttcct actccagctt ggcagtgccc
     1621 tgaccgccgc cggtgaagcc gttaacgatg ttcaagatca cctccaagcg gttgcggttg
     1681 cgtacggggc gaacgaagcc cggtttgtcg tgctcccgac tttcctggtg gtcgccctcg
     1741 atccgaggcg gccagccacc ctcgagccca cttcacagct cagcggcgtg ctccgcctcg
     1801 accagacttc cggtctgttc gacatcttga aaaaggccac aaaggccgag atcgacccc
//
LOCUS       DATITW010000566         1518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_268389, whole genome shotgun sequence.
ACCESSION   DATITW010000566 DATITW010000000
VERSION     DATITW010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268389"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VLS86_07870"
     CDS             <1..546
                     /locus_tag="VLS86_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50450.1"
                     /translation="VLALLDNLPGVAVEPEPRHYMALAVTILGIGLLVGSFLGRARWL
                     ILVGVILVPTMLFSPVFEYDWNSDDFQLSETPVTFAEVEESYTLEIGNLLLDLTELPW
                     DGRTIDLEISVDAGNVELRLPEGVGLTGHAEVTAGRVAGPGRETSGLGNPGLAFNNPG
                     PSGTVDLDAQVDIGNIEITRG"
     gene            565..795
                     /locus_tag="VLS86_07875"
     CDS             565..795
                     /locus_tag="VLS86_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50451.1"
                     /translation="MKTHDFDAVSFIAGLAFTIIGLVFLIPQTPQEIIDVLLDSGAWF
                     WPIALLVVGLAVIIPVLVPKKEEEPIESGSED"
     gene            complement(792..>1518)
                     /locus_tag="VLS86_07880"
     CDS             complement(792..>1518)
                     /locus_tag="VLS86_07880"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSO50452.1"
                     /translation="IFSGVFGVGGGIIMVPLLVAVGMERHRAHATSLAAIIPIAVAGA
                     VAFGLSGEIDLGLGIYIGLGGVVGSVLGASLMNRMSSRSLSVFFGIVLLAAGIRMIAS
                     GDPLPGSGEFGEPAQIAIAIGIGLISGFFAGVAGIGGGAVIVPATVLLLGFTQHVAQG
                     TSLVAIILTAIAGSVVNFRNKRVRLEDGLAAGGAGVVGSIVGAQIALGADGRTLSVLF
                     GALIVVLSARTLYQAIRAPKAVA"
BASE COUNT          311 a          509 c          446 g          252 t
ORIGIN      
        1 gtgctggcgc ttctcgacaa tctcccaggg gtggccgtcg aacccgaacc acgtcactac
       61 atggccctgg cggtgaccat cctcggcatc gggctcttgg tcgggtcgtt tctcggccgt
      121 gcccgctggc tgatcctggt cggcgtgatc ttggtcccga cgatgctgtt ctcccccgtc
      181 ttcgagtacg actggaacag cgacgacttc cagctctcgg agacgccggt gacctttgcc
      241 gaggtggagg agagctacac gctggaaatc ggcaacctcc tcctcgacct gaccgagctc
      301 ccctgggatg gcaggaccat cgacctcgag atatcggtag atgccggcaa cgtcgagctc
      361 agactccccg agggcgtcgg gctcaccggc cacgcggaag tcacggcggg tcgagtggca
      421 ggccctggtc gggagacatc aggtctcggc aatccgggcc tggccttcaa caaccccggc
      481 ccgtcgggca cggttgacct cgatgcccaa gtggacatcg gcaacatcga aattacccgt
      541 ggctgatcga aaggatccat cgacatgaag acacacgact tcgatgcagt ctcgttcatc
      601 gccggacttg cattcaccat catcgggctc gtcttcctga taccccagac ccctcaggag
      661 atcattgacg tcctcctcga ctctggcgcc tggttctggc cgatcgccct gctcgtggtc
      721 gggctcgcgg tgatcatccc tgttctggtg cccaagaagg aagaggagcc gatcgagtcg
      781 ggtagcgagg actaggcgac agccttcggg gcccgaatcg cctggtagag agtccgagcc
      841 gagagaacca cgatcagagc cccgaacaga actgacaagg tacggccgtc ggctccaagg
      901 gcgatctgag cccccacaat cgagcccacc accccggcgc caccagcagc gagtccgtcc
      961 tcgagacgga ctcgcttgtt tctgaagttg acgacactcc cggcgatagc ggtgaggatg
     1021 atcgccacca gcgaggtccc ttgtgccacg tgctgggtga acccgaggag aagcacagtg
     1081 gcgggcacga tgacggctcc ccctccgatc ccggccaccc ccgcaaagaa cccggatatc
     1141 aggccgatgc caatcgcaat ggcaatctgg gccggttcgc cgaattcccc cgaacccggc
     1201 aacgggtcgc cactcgcgat catccggatc ccggcggcca acagcacgat cccgaagaag
     1261 acgctcaacg agcgtgagct catacggttc atgagggagg cgcccagcac ggaccccacc
     1321 accccaccca aaccgatata gataccgagg ccgagatcga tttctccgga gaggccgaaa
     1381 gcaactgcgc ctgcaaccgc gattggaatg atcgctgcca gggaagtggc gtgggctcga
     1441 tgcctctcca tgccgaccgc caccagaagc gggaccatga ttatcccccc gccaactcca
     1501 aaaacacctg agaagatg
//
LOCUS       DATITW010000567         2763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_108710, whole genome shotgun sequence.
ACCESSION   DATITW010000567 DATITW010000000
VERSION     DATITW010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108710"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VLS86_07885"
     CDS             <1..213
                     /locus_tag="VLS86_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50453.1"
                     /translation="TLMTGTLGLGLGVASRLTALLGGKLSYQRFAQKSYFTVTRPLLA
                     AEVEAAEAGDEGEEAVVADVIRAMSA"
     gene            210..1388
                     /locus_tag="VLS86_07890"
     CDS             210..1388
                     /locus_tag="VLS86_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50454.1"
                     /translation="MRRLVGAVLVAMLFSLTSIALASDSGYYKDRFSSIGWAGSDGSL
                     HWSGPWSEVNDDGDEKKGSVRVVSSGHCAEGNCMRIAGGITLLDDIGARRTADTKDME
                     ELSLCYDVKNVTSALPLGLTELRVQVRGDGGWVTVAEYDLGSDFSGHYTHDDLDDFES
                     KSFRVRFLVTGPAMTSEVFIDNVEVEGELDDEPGGTTTTTTSSTTSSTTSTTIGETTT
                     TTAAGSTTSTSTPVTTTTQPRASTTTTTTTTTTTAGEPSSATTVTTTTPGDTARDQTI
                     APDISLSTTTTTTTPGNSTDSGAISSVDEDGSDGLGTSSGGSGIKAAARGVQANFEGD
                     LFGDARTVSSLGGVDLQADYTIAAEVIRSSWAWMVLLGLVMAWAIVSRLDRNRPDLDV
                     "
     gene            1452..1973
                     /locus_tag="VLS86_07895"
     CDS             1452..1973
                     /locus_tag="VLS86_07895"
                     /inference="COORDINATES: protein motif:HMM:NF039198.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HSO50455.1"
                     /translation="MDVVFDLLIIFVLRLLDVGMATVRIVLLGRGRRGPATILGFFES
                     LVWVLAIARVFDGLDDPARMIAFALGFAAGTYVGSLVEEWMALGQALIRVVAPVATEP
                     VAPLLRERGYGATVVNGDGLSGEVRLTFCVVPRKEVYAVTKLIHGANPAAYVTIDQTS
                     TIDLARHAGDVNK"
     gene            2100..2648
                     /locus_tag="VLS86_07900"
     CDS             2100..2648
                     /locus_tag="VLS86_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007472259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSO50456.1"
                     /translation="MDHLNRRDAELASRVASGDRDALAEVFNEIGGAVKSVAFRVLRE
                     EALAEDVVQDTFVGFWRAPGKYDPERGALRSFLLTIAHRRAVDIVRSEEARARRELQP
                     PDPDHFDLVDEVLARDLSNEVRQALTSLAEGEREAISMAYFGGLSYVEVAERLGAPEG
                     TVKSRIRSGMRKLASSLEGVTP"
BASE COUNT          501 a          907 c          881 g          474 t
ORIGIN      
        1 acgttgatga ccggcaccct cggtcttggt ctcggagtcg cctcgcggct caccgccctg
       61 ctcggcggaa agctctcgta tcagagattc gcccagaagt catacttcac tgtcacccgt
      121 cccctgctcg ccgccgaggt cgaagccgcc gaggcgggag acgagggcga agaggctgtc
      181 gtagccgacg tgatccgggc gatgtcggca tgagacggct ggtgggggcg gtcctggtcg
      241 caatgctctt cagcctcacc tcgatcgccc tggcatcgga ttccgggtat tacaaggatc
      301 gcttctcgtc gatcgggtgg gccgggtctg acggctcgct ccattggtcc ggcccatgga
      361 gcgaggtgaa cgacgacggc gacgagaaga agggcagtgt tcgggtggtc tcatcgggtc
      421 actgcgccga gggcaactgc atgcgaatcg ccggcgggat cacgctgctc gacgacatcg
      481 gagcccgacg caccgccgac accaaggaca tggaggagct gagtctctgc tacgacgtga
      541 agaacgtcac ctcggccctg ccgctgggcc tgaccgagct tcgggtgcag gttcgaggtg
      601 acggggggtg ggtcaccgtt gccgagtacg acctgggaag tgacttctcc ggccactaca
      661 cccacgatga cctcgacgac ttcgaatcga agagttttcg ggtccggttc ctggtcacgg
      721 ggccggcgat gaccagcgaa gtgttcatcg acaatgtcga ggtggaagga gagctggacg
      781 atgagcccgg cggcaccacc accaccacga ctagctcgac caccagctcg actaccagca
      841 ccacgatcgg cgagaccacg accaccaccg cggccggctc gacgaccagc accagcaccc
      901 cggtcaccac caccacccag ccgagagcga gcaccaccac caccaccacc accaccacca
      961 ccacggcagg agagccatcc tcggcgacca cggtcaccac cacgacacca ggtgacacgg
     1021 cccgcgacca gacgatcgca cccgatatct cgctatcgac cacgactacg acgaccactc
     1081 caggcaacag caccgattcc ggggcgatca gctccgtcga cgaagacgga tctgatggcc
     1141 tcgggacgtc ctcgggagga agcgggatca aggctgccgc caggggcgtc caggccaact
     1201 tcgagggcga cctcttcggc gatgcccgga cggtgtcctc gcttggtggc gtcgatcttc
     1261 aggctgacta cacgatcgca gccgaggtca tcaggtcctc ctgggcctgg atggtcctgc
     1321 tcgggctggt gatggcatgg gccatcgtct cccgtctcga tcgcaaccgc cccgacctcg
     1381 acgtctgatt ccctcggcgc caggctcatt tgccgggatc gttgggcctc ggtgcctacc
     1441 gtgaacccgt gatggacgtc gtcttcgacc tcttgatcat cttcgtcctg cgtctgctcg
     1501 atgtggggat ggccacggtt cgcatagtcc tccttggacg tggtcgccgg ggcccggcga
     1561 ccatcctcgg gttcttcgaa tcgctggtct gggtgctggc catcgcccgg gtcttcgacg
     1621 gtctcgacga tccggcgcgc atgatcgcct tcgccctcgg gttcgccgcc ggcacctacg
     1681 tcggctctct cgtcgaagag tggatggcct tgggccaggc tctgatccgg gtagtcgccc
     1741 cggtggcaac cgaaccggta gcgcccctgc tccgggagcg aggctacggg gcgacagtgg
     1801 tcaacggcga cgggttgagc ggcgaggtga ggctgacctt ctgtgtcgtc cccagaaagg
     1861 aggtctatgc cgtcaccaag ctgatacatg gcgccaaccc cgccgcctat gtcaccatcg
     1921 accagacctc cacgatcgac ctggcccgtc acgccgggga cgtcaacaaa taggagtcaa
     1981 tccggagggc gccccggtgc cgaataccgg gtggaagaag gaggtcgccg atttggcaga
     2041 cgaatctccg cttggcccca cctctatcat cgggaaccca cggccgaccg gagctgtatt
     2101 tggaccattt gaatcgacgc gatgcggaac tcgcctcgcg agtcgcgtcc ggcgatcgcg
     2161 atgcactggc cgaggtgttc aatgagatcg gcggagctgt gaaatcagtg gccttccggg
     2221 tcttgagaga ggaagctttg gccgaggacg tggtgcaaga cacatttgtc ggtttctgga
     2281 gggctcccgg gaaatacgac ccggagcggg gggcgctccg ctcgtttctc ctcaccattg
     2341 cccatcgcag agcggtcgac atcgtccgat ctgaggaggc gcgagccaga cgagagctgc
     2401 agccgccgga ccccgaccat ttcgacctcg tcgacgaggt cctggcccgc gatctgtcca
     2461 acgaagtccg ccaggccctc acctcactgg ccgagggcga aagggaggcg atctccatgg
     2521 cttacttcgg tgggctgagc tatgtcgagg ttgccgagcg actcggcgcc ccggagggga
     2581 cggtaaagag ccggatccgg agcggcatga gaaagctcgc ctcctcgctc gagggtgtga
     2641 cgccatgatg acccacgagc aagtcttcga gctcttgccg ctgcatgccc tggacgcact
     2701 ccaaggcgac gagctcagcg aggtcgaatc acatctcacc acgtgcgaca tctgcctggc
     2761 gga
//
LOCUS       DATITW010000568         1596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_28480, whole genome shotgun sequence.
ACCESSION   DATITW010000568 DATITW010000000
VERSION     DATITW010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28480"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VLS86_07905"
     CDS             <1..1090
                     /locus_tag="VLS86_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HSO50457.1"
                     /translation="LTEMLPNKEFQGMKSRFMRLVGVVVVLGVLISACGGDGADESTT
                     TAAGEEPGSTAGEGGGGQIVVSGSSTVEPITARVAEAFGGLNPDVAISVDGPGTSDGF
                     VLFCNGETDVSDASRAITDEELATCQANGVEPVEIKVAIDGLSVITSAANEAVSCLAF
                     SDLYALLGIESTGFGNWSDANDLAAEIGAPNAPYPDAPLTITAPGEESGTFDSFVELV
                     LEDIAEERGQDPNPRPDYVASPNDNVIIEGISSNETSLGWVGYAFYVENTDVVKALAV
                     DGGDGNCVEPTPETIAANEYPISRPLFIYVDAAKLDAKPELTAFVDYYVSDEGLAEVG
                     NAGYVDLTPEEIDEWRANWDARTVGKVD"
     gene            1177..>1596
                     /locus_tag="VLS86_07910"
     CDS             1177..>1596
                     /locus_tag="VLS86_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HSO50458.1"
                     /translation="MTVEAPSEISRSLRGDPRRQRREAFWHVFFLGAAAVSIVITIAI
                     IFSLVGEAWTFISNVDLSSLWDIGWFPRRGLFDIRTIVVGTLLVAGIAMLVATPLGIG
                     AAMYLSEYARPRVRRVLKPALEILAGIPSVVLGYFALT"
BASE COUNT          288 a          497 c          520 g          291 t
ORIGIN      
        1 gttgacggaa atgttgccta acaaggagtt ccaagggatg aaatcccgat tcatgcgttt
       61 ggtgggcgtg gtcgtcgttc tcggcgtcct gatctcagcg tgcggtggtg acggtgcgga
      121 cgaatccacg accacggccg ctggtgaaga gcccggctcc accgcaggcg aaggtggagg
      181 cggccagatc gtggtctccg gatcgtcgac tgtcgagccg atcacggcca gggtcgcaga
      241 ggccttcgga ggcctgaacc ctgacgtcgc gatcagcgtc gatgggccgg gcacctccga
      301 cggctttgtg ttgttctgca acggcgagac cgacgtctcc gacgcttccc gggccatcac
      361 cgacgaggag ctggcgacct gtcaggccaa cggagtggag ccggtcgaga tcaaggtggc
      421 gatcgacggc ctgtcggtca tcacttcggc agccaacgag gccgtctcgt gtctggcctt
      481 cagtgacctg tacgcgttgc tcggaatcga gtcgaccgga ttcgggaatt ggtccgatgc
      541 caatgacctc gcggccgaga tcggggcgcc caacgcccca tatccggacg cccctttgac
      601 catcaccgca ccgggtgagg agtcgggcac attcgacagc ttcgtcgagt tggtgctgga
      661 ggacatcgca gaggagaggg gccaggatcc aaacccccgc cccgactacg tggcctcccc
      721 caacgacaac gtgatcatcg aggggatatc cagcaacgag acctcactgg gatgggtcgg
      781 ttacgccttc tacgtcgaga acacagacgt ggtgaaggca ctggcggtcg acgggggtga
      841 cggcaactgt gtcgagccca cgccggagac gattgcggcg aacgagtatc cgatctcccg
      901 tcccctgttc atctacgtcg acgccgccaa gctcgatgcc aagccggagc tgaccgcctt
      961 cgtcgactac tacgtgtccg acgagggact cgcagaagtc ggaaacgccg gctacgtgga
     1021 tctcactccg gaagagatcg acgagtggcg ggccaactgg gatgcgcgca ccgtgggcaa
     1081 ggtcgactga gagcggatgc agagtgggag ccgacgtgcg accagagtcg ggcgcacgtc
     1141 ggctctaccc tctagctcct ctttgagaag cagacgatga ctgtcgaagc gccatcagag
     1201 atctcgagat ctctccgggg agacccccga cggcaacgtc gtgaggcttt ctggcacgtg
     1261 ttcttcctcg gggcggcggc ggtctcgatc gtgatcacca tcgccatcat cttctccctc
     1321 gtcggtgagg catggacctt catcagcaac gtggacctgt cgtcgctgtg ggacatcggc
     1381 tggttcccga gaaggggcct tttcgacatc cgcaccatcg tggtcgggac cctgctggta
     1441 gccgggatcg ccatgctggt ggccaccccg ctgggcatcg gagccgccat gtacctctcc
     1501 gagtacgcac gcccccgggt ccgcagggtc ctcaaacctg ccctggagat cctggcggga
     1561 atcccgtcgg tggtcctggg ctatttcgcc ctcacc
//
LOCUS       DATITW010000569         1773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_268441, whole genome shotgun sequence.
ACCESSION   DATITW010000569 DATITW010000000
VERSION     DATITW010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268441"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1677)
                     /locus_tag="VLS86_07915"
     CDS             complement(37..1677)
                     /locus_tag="VLS86_07915"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSO50459.1"
                     /translation="MRRPLILAVAVCLVAGSMISPAIGQTLPPGGTFVDDDGNLHEGA
                     IEAISAAGITTGCGPALYCPTLPVTREQMAVFLIRALGESPSTASGTFPDVPPGPFAG
                     HIERIAELGIATGYPDGTFGPANPISRGEMAVMLMRALDLAPGTNSGTFSDVPGGLFF
                     TGAVGAIAAAGITTGCGGGKFCPFDQVLRDQMASFISRAYGLTPIIPPPRVTELAVVE
                     VAGGFEAPVFVASPPGDSRLFVVDQDGRIWIIQGGSRLATPFIDLRGPVGFGGEQGLL
                     GMAFHPDYASNRRFFVNYTDNAGDTRIVEYEASASNPNVADVGSARELLMIDQPASNH
                     NGGWLGFGPDGILYIPMGDGGGGNDQFGNGQDPDSLLGSILRLDVDAAAPGAEIWAKG
                     VRNPWRNAWDGTNLYIADVGQGQREEVTVLTTGSAGANLGWPVVEGTRCLSGSTCNTA
                     GFTPPVYEYTHSDGCSITGGYVYRGSAMPTLAGTYFFSDYCSGWLRSFRYVGGVATDV
                     REWTDLGSVGQVLSFGVDSAGEMYITVADGRVLKLVPG"
BASE COUNT          329 a          590 c          551 g          303 t
ORIGIN      
        1 cttccgtctt ccgccttccg ccttccgtct ggtaactcag cccgggacca gcttcaggac
       61 ccggccgtcg gcaacggtga tgtacatctc accggcggag tcgaccccga aggacagcac
      121 ctggcccacg ctgcccaagt ccgtccattc acggacatcg gtcgctaccc cgccaacgta
      181 ccggaaagat cggagccatc ccgagcagta gtcgctgaag aagtacgttc ctgcgagagt
      241 cggcatggcc gaaccgcgat agacgtagcc acccgttatc gagcagccat cactgtgggt
      301 gtactcgtag accggtggcg taaagcccgc cgtgttgcag gtcgacccgc tgaggcagcg
      361 ggtcccctcc accaccggcc aaccgaggtt ggccccggcc gatccggtcg tcagtaccgt
      421 cacttcttcg cgctggccct gaccgacgtc ggctatgtac aggttggtgc cgtcccacgc
      481 attgcgccag ggattgcgga cgcccttggc ccagatctcg gccccgggag cagcagcgtc
      541 aacgtcgagc ctgaggatcg atcccagaag gctgtcggga tcctggccat tgccgaattg
      601 gtcattgccg ccgccgccat cccccatcgg gatgtagagg atgccgtcgg gtccgaagcc
      661 gagccaccca ccattgtgat tcgaggctgg ctgatcgatc atcagcaact ctcgagccga
      721 tcccacatca gcgacattcg gattggatgc cgaggcttcg tactcgacga tacgggtatc
      781 acccgcgttg tcggtgtagt tcacgaagaa gcgacggttg ctggcgtagt cgggatggaa
      841 ggccatgccc agcagtccct gctccccgcc gaacccgacc ggaccgcgca gatcgatgaa
      901 cggagtggcc aggcggctgc cgccctgaat gatccagatt cgaccgtcct gatcgaccac
      961 gaacaagcgt gagtcccccg ggggcgaagc aacgaacacc ggcgcttcga atcccccggc
     1021 gacctcgacc actgcgagtt cggtcacgcg tggcggaggg atgatgggag tcaggccata
     1081 ggcgcgggat atgaagctgg ccatctggtc gcgcagtacc tgatcgaagg ggcagaattt
     1141 cccaccgccg catccggtgg tgatgccggc cgcggcgatg gctcccactg cgccagtgaa
     1201 gaacaggcca ccgggaacgt cggagaacgt tccgctgttg gtcccggggg ccagatcgag
     1261 cgcccgcatc aacatcactg ccatttcgcc gcgggagatg gggttggccg gaccgaaagt
     1321 gccatcggga taaccggtgg cgatcccgag ctcggcaatg cgttcgatgt ggcccgcgaa
     1381 cggacccggg ggaacatccg ggaacgtgcc ggaggccgtc gaggggctct ccccgagtgc
     1441 tcgaatcaga aagaccgcca tctgctcgcg agtcaccggc agggtcgggc aatagagcgc
     1501 aggcccgcaa ccggtcgtta tcccggctgc agagatcgct tcgatcgctc cctcgtgcag
     1561 gttgccgtca tcgtcgacaa acgtgcctcc gggcggcagc gtttgtccaa tcgccggaga
     1621 gatcatgctc cccgccacca ggcagactgc cactgccaga attagtggtc ttctcatcga
     1681 tccctccgat cggtcggtcg cccgcccgga ggctatcaac tcgccgttct ttgacgggat
     1741 cagacctcac ggccctcagt gtcggccgcc gaa
//
LOCUS       DATITW010000570         3844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_429077, whole genome shotgun sequence.
ACCESSION   DATITW010000570 DATITW010000000
VERSION     DATITW010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429077"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2348
                     /locus_tag="VLS86_07920"
     CDS             <1..2348
                     /locus_tag="VLS86_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HSO50460.1"
                     /translation="IDGGPRQPLDLDLAVIHHETVELGRDSQVPPGTAPSLDLAPDQM
                     EAVSHLVGPARVIAPAGSGKTRVLTARVRHLLEDRGIEPELITALAYNRRAAGEMVDR
                     LPAGRLNVRTIHSLGWEILRMAQPHLHLIDEREQRRRLEPITTAPPRANTDVIGPYLE
                     ALDEVRIGLRDPEEVEAERDDVPGFVDTFRRYRESLERAGEADHAEQIYGAIETLCRF
                     PDLRAHWQSQCRHLLVDEFQDLTPAYLLLIRLVAGPGLNVFGVGDDDQVIYGYAGADP
                     GFLIDFEELFPGATAHALEVNYRCPADVVESAGNLLGYNRRRVGKAIRAVSAAGGLVV
                     SQAPGAELGVVAADRIAALIEEGAQPAAIAVLSRVNSSLLPVHVALSERGIAFHSPLS
                     ASVLDRTLLRAALSWIRIAVEPDAMSRNDLFEAIRRPGRGITRLFGELIGRRRGPFAL
                     EDLIILGRDLDGRRGDRWNEFCEDILVASRATLSTSGLLDVLASHVGLDRAAAALDAG
                     RTRADRAAQGDDLTALRRVAAMGPAPRVFEPWLRERLISSTAPDGVTLSTVHRVKGLE
                     WDHVIVFGVDSGSMPHELSADIEEERRVFHVALTRGRRSVTLLSDRARPSRFLAEIDG
                     SAPVPVEPPPPRADRAAALPPSDALFVGVGDELAMGGGYRGNVDEIRVTGVLVRLAET
                     EATMAVPWGEKVTRSGLDGRLTPGSGGADPDLIDRLREWRLDQARSQGVPAYVVFNDR
                     TLEAIATLRPSSETALLDVPGIGPAKLDAYGDQLLDLIALD"
     gene            2450..3781
                     /locus_tag="VLS86_07925"
     CDS             2450..3781
                     /locus_tag="VLS86_07925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HSO50461.1"
                     /translation="MNSSSNRHKIHPRLASELARLGLDENRAPDQETWDRLLGALNQH
                     YLESEQLNRELVDATLGWSSFENLFRISPVPMIEQDYTVLERWMDGLRERGVASIRDH
                     LGEDIEAIREIVPMIRIVAANPAAVSAVGVPLEALIGPIDPLIVNEGSQNGWLTQLDA
                     VFHGEPEAHASYTAITPSGQTYDAESILAAPLIDGVPDYSRAVFTVIDVTDHRNEERR
                     MQELVQAKNRFLASVSHEIRTPLTAILGFGRILEEDRTLNEDDRSLMVSSIVQHSQEV
                     ANLVEDLLVAARADMGQLEVVNTTLDVLAQVNQTLNAGGSFTTDVTLESESDSVRAIG
                     DPMRVRQIVRNLLSNAERYGGSAVSVIVSRSGRQVFVDVADDGPALSKSEEEKIFQPY
                     YRVHDSPSLPGSVGIGLTISRQLAELMGGSLEYRHQDGASCFRLSLRAAKR"
BASE COUNT          629 a         1293 c         1269 g          653 t
ORIGIN      
        1 ggatcgacgg tggcccccgg cagccgctcg atctggatct ggccgtaatc caccatgaga
       61 ccgtcgagct gggaagggat tctcaggtgc cgccgggcac ggcaccgtcg ctggatctgg
      121 cgccggacca gatggaagcg gtgtcacacc tggtcgggcc ggcgcgggtc atcgccccgg
      181 ccgggtcggg gaagaccagg gtcctgacgg ccagggtcag gcatctgctc gaggatcgtg
      241 ggatcgagcc cgagctcatc accgccctgg cctacaaccg acgggccgcc ggggagatgg
      301 tcgaccggct tcccgccgga cgtctcaatg tccgcaccat ccactcgctg ggctgggaga
      361 tcctgcgcat ggcccagcca catctgcacc tcatcgacga gagggagcag cgtcggcggc
      421 tggagcccat caccaccgct ccgccacggg ccaacaccga cgtcatcggc ccctatctcg
      481 aagcgctgga cgaagtcagg atcgggctcc gcgacccgga agaggtcgag gccgaacgcg
      541 acgacgtccc cggattcgtc gacaccttcc gccggtaccg cgagagcctg gagcgcgccg
      601 gtgaggccga ccatgccgag cagatctacg gggcgatcga gactctttgc cgcttccccg
      661 acctcagggc ccattggcag agccaatgtc gccatctcct ggtcgacgag ttccaggacc
      721 tgaccccggc gtacctgctc cttatccggc tggtcgccgg gccggggctg aacgtgttcg
      781 gggttggtga cgacgatcag gtgatctacg ggtacgccgg cgccgatccc gggttcctca
      841 tcgacttcga agagctgttc cccggggcca ctgctcacgc cctcgaggtc aactaccggt
      901 gtcccgccga cgtcgtcgag tcggcgggaa acctgctcgg atacaaccgg cggcgggtcg
      961 gcaaagcgat tcgggcggtc tccgctgccg gtggcctcgt ggtgtcccag gcgcccggcg
     1021 ccgagttggg cgtggtggcc gcggatcgaa tcgccgccct catcgaggaa ggggcgcagc
     1081 ccgccgcgat cgcagtcctc tcccgggtga actcgagtct gctccctgtc cacgtcgccc
     1141 tctccgagcg cggcatcgcg ttccacagcc cgctatcggc gagcgtcctc gatcgcacgc
     1201 tgctccgcgc agcactgtct tggatccgga tcgcggtcga acccgatgcg atgagccgca
     1261 acgacttgtt cgaggcgatc cgacggcccg gtcgtgggat cacccggctc ttcggcgagc
     1321 tcatcggtcg acgtcgcggc ccgtttgccc tggaggatct catcatcctc ggtcgtgatc
     1381 tcgacgggcg tcgcggtgac cggtggaatg agttctgtga ggacattctg gtggcttcga
     1441 gggccacgct ctccacgtcg ggtctcctcg acgtgctggc atcccatgtc gggctcgacc
     1501 gagctgcggc cgcgctcgat gccgggcgga cccgcgccga ccgggccgct cagggcgacg
     1561 acctcaccgc attgcgccgg gtggcagcga tggggccggc cccgagagtg ttcgagccgt
     1621 ggctgcggga gcggctgata tcgtccaccg cacccgacgg ggtcaccctg tccacggtgc
     1681 acagagtcaa gggtttggag tgggatcacg tgatcgtgtt cggcgtcgac tcgggatcga
     1741 tgccacacga gctgtctgcg gacatcgagg aggaacgccg cgtgttccat gtcgccctga
     1801 cccgtggccg gcgttcggtc accctcctct ccgaccgtgc ccgcccgtcc cggttcctgg
     1861 ccgagatcga tggcagcgca cctgttcccg tcgagccacc gccgccccgg gccgatcgag
     1921 ctgcggctct acctccgtcc gacgcgctgt tcgtcggagt cggcgatgag ttggcgatgg
     1981 gcggcgggta ccggggcaac gtggacgaga tccgggtgac gggagtcctg gtcagactgg
     2041 ccgagaccga ggcgacgatg gcggtcccct ggggggagaa ggtgaccaga tccggcctcg
     2101 acggccggct cacgcccgga tcgggcggag ccgaccccga tctgatcgat cgactcagag
     2161 agtggcggct ggaccaagct cggtcacagg gtgtgcccgc ttatgtcgtg ttcaacgacc
     2221 ggacactcga ggccatcgcc accctgcgcc cttcgtccga gacggctctg ctcgatgtcc
     2281 ccggcatcgg ccccgccaag ctcgacgcct acggagacca attgctcgac ttgatcgcat
     2341 tggactgagc gacgtttcgc ggggcggcga ctcaacaaaa cccacgagaa tgccgatatc
     2401 gggagatgcc tgagtcaccc gaagtcgtcg ccctgcagct cccgaccccg tgaactcgag
     2461 ctcaaaccga cacaagatcc atcctcgact cgcttccgag ctggcacggc tcgggctcga
     2521 cgagaaccgg gcccccgacc aggagacctg ggatcggctc ctgggcgcgt tgaaccagca
     2581 ttacctcgag tcggaacagc tcaaccggga gttggtcgac gccactctgg gatggtcgag
     2641 tttcgagaac ctgttccgga tctcgccggt gcccatgatc gaacaggact acaccgtcct
     2701 ggagagatgg atggatggtc tgcgtgaacg gggtgtggcc agcatccggg accacctcgg
     2761 tgaagatatc gaagccatcc gggagatcgt ccccatgatc aggatcgtgg cggccaatcc
     2821 cgccgcggtg tcggcggtcg gtgtccccct cgaagcgctg atcgggccca tcgaccccct
     2881 gatcgtcaac gagggctccc agaacggctg gctgacccag ctcgatgcgg tgttccatgg
     2941 cgagcccgag gcccatgcct cctataccgc gatcacgccc agcggccaga cctacgacgc
     3001 cgagtcgatt ctcgccgcgc cactcatcga cggcgtgccc gactactccc gggccgtgtt
     3061 caccgtcatc gatgtcaccg atcatcgaaa cgaggaacgc cgcatgcagg agctggtaca
     3121 ggccaagaac cggttcctgg cctcggtcag ccatgagatc aggactccgc tgactgcgat
     3181 cctcggcttt ggccggatcc tcgaagagga ccggacattg aatgaggacg atcgctcgct
     3241 gatggtgtcg tcgatcgtgc agcattccca ggaggtggcc aacctcgtcg aggacttgct
     3301 ggtggcggca cgagccgaca tgggccagct ggaagtggtc aacaccaccc tggacgtctt
     3361 ggctcaggtg aatcagacct tgaatgccgg tgggtccttc accaccgatg tcaccctcga
     3421 gtcagagtcc gactcggttc gggcgatcgg ggacccgatg cgggtgcgcc agatcgtgcg
     3481 caacctcctc agcaatgccg agcgctacgg aggctccgcg gtcagcgtga tcgtgtcgag
     3541 gtcaggccgt caggtgttcg tcgacgtggc cgacgacggc ccggctctct ccaagagcga
     3601 ggaggagaag atcttccagc cctactaccg ggtgcatgac agtcccagcc ttcccgggtc
     3661 ggtcggaatc ggtctcacca tctcccgcca actggccgag ttgatgggtg gcagcctcga
     3721 ataccggcac caggatggag ccagctgctt tcgcctctca ctccgcgcag ccaagcgctg
     3781 acacatcggg ctcggggcgg cggggtgtgg agctggttga atccggtccg tgttcgcccc
     3841 actg
//
LOCUS       DATITW010000571         1763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_108931, whole genome shotgun sequence.
ACCESSION   DATITW010000571 DATITW010000000
VERSION     DATITW010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108931"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1510
                     /gene="rho"
                     /locus_tag="VLS86_07930"
     CDS             23..1510
                     /gene="rho"
                     /locus_tag="VLS86_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HSO50462.1"
                     /translation="MTTRAQLQSKSIDELRQIADAVGIETDGLQKSKLITALVDAGQV
                     AEASPTEDVDLPQATPRIERVSQNGEGEPDSDEGEDQRPDQRQGARSDYGDDQRQGGN
                     RNRNRRRRSGGREDEYIPEEDLETREGLLDTLPEGYGFLRVTGYLPGDKDVYVSANQV
                     RKFRLRKGDIVSGPIRPPRSKEKFPAVVRIDKVNGMTSDEAMKRPKFESLTPLFPDQR
                     LRLEIDGRPEGILTRIVDLIAPIGKGQRGLIVSPPKAGKTTVLQEMAQAITTNNPECY
                     LMVVLVDERPEEVTDMQRSVKGEVIYSTFDRPAEEHTQVSELALERAKRLVEMGTDVV
                     ILLDSITRLARAHNLATPASGRILSGGVDSQALYPPKRFFGAARNIEEGGSLTILGTA
                     LVETGSKMDEVIFEEFKGTGNMELRLDRKLADKRIYPAIDIEASGTRKEELLFDQKEL
                     AQVWKLRRVLLALEPGAALELLIDRLKTTKSNADFLAEVAKSSGS"
     gene            1524..>1763
                     /gene="rpmE"
                     /locus_tag="VLS86_07935"
     CDS             1524..>1763
                     /gene="rpmE"
                     /locus_tag="VLS86_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HSO50463.1"
                     /translation="MKTDIHPTYAETHVTCSCGNTFTTQSTKPGEMHIELCNECHPFF
                     TGKQKLVDSGGRVERFQKRYGKTAFTKAGDPVDASA"
BASE COUNT          372 a          577 c          555 g          259 t
ORIGIN      
        1 gcgaagactc gcgaaggatc caatgaccac acgagctcaa ctccagagca agtccataga
       61 cgaactcagg caaatcgccg acgccgttgg catcgagacc gatggcctgc agaagtccaa
      121 gctgatcacc gccctcgtag atgcggggca ggtggccgag gcctcgccca ccgaggacgt
      181 cgatcttccc caggccacgc cccgaatcga gagggtgtca cagaacggag agggcgagcc
      241 cgactccgat gagggcgagg accagcgtcc cgaccagcgt caaggcgccc gcagtgacta
      301 cggcgacgac cagcgccagg gcggcaatcg gaaccgcaat cgtcgccgcc gcagcggggg
      361 gcgtgaggac gagtacatcc ccgaagagga cctcgagacc cgggaaggcc tcctcgacac
      421 ccttcccgag ggatacggct ttctgagagt caccggttac ctgcccggtg acaaggatgt
      481 ttacgtttcg gccaaccagg tccgcaagtt ccgtctccgc aagggagaca tcgtctccgg
      541 cccgatccgc ccgccgcgat ccaaggagaa gttcccggca gtggtccgga tcgacaaggt
      601 caacgggatg acctcggacg aagcgatgaa gcggccaaag ttcgagtcgc ttacccccct
      661 cttccccgac cagcggcttc gcctggagat cgacgggaga cccgagggga tcctcacccg
      721 gatcgtcgac ctcatcgcgc cgatcgggaa gggtcagcgt gggctcatcg tctccccacc
      781 caaggcgggg aagacgaccg tgctccagga gatggcgcaa gccatcacca ccaacaaccc
      841 cgagtgctac ctgatggtgg tcctggtcga cgagcgcccc gaggaagtca ccgacatgca
      901 gaggtcggtc aagggcgagg tcatctactc caccttcgac cgccccgccg aggagcacac
      961 ccaggtctcc gagctcgccc tggagcgggc gaagcgtctg gtcgagatgg gcaccgacgt
     1021 ggtgatcctg ctcgactcga tcacccgtct cgcccgtgcc cacaacctgg ccacgccggc
     1081 atcggggagg atcctctccg gtggtgtcga ttcccaggcc ctgtacccgc caaagcggtt
     1141 cttcggtgcg gcgcggaaca tcgaggaggg agggtcgttg accatcctcg gcaccgccct
     1201 cgtcgagacc gggtccaaga tggacgaggt catcttcgag gagttcaagg gcaccgggaa
     1261 catggagctt cgtctcgacc gcaagctcgc cgacaagcgg atctacccgg ccatcgacat
     1321 cgaggcatcg ggaacaagga aggaagagct tctgttcgac cagaaggagc ttgcgcaggt
     1381 ttggaagctg cgcagggttc tcctggccct cgagccggga gccgccctcg agcttctcat
     1441 cgaccgattg aagaccacga agtccaacgc cgatttcctc gccgaggtgg cgaagagcag
     1501 cggcagctag ataggagcca gcggtgaaga ccgacattca tccgacgtac gccgagaccc
     1561 atgtgacctg ctcgtgtggc aacaccttca cgacccagag caccaagccc ggcgagatgc
     1621 acatcgagtt gtgcaatgag tgccacccgt tcttcaccgg caagcagaag ctggtcgaca
     1681 gcggtggccg ggtggagcgc ttccagaagc gttacgggaa gactgccttc accaaggccg
     1741 gcgacccggt cgatgcgagc gca
//
LOCUS       DATITW010000572         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_308773, whole genome shotgun sequence.
ACCESSION   DATITW010000572 DATITW010000000
VERSION     DATITW010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308773"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VLS86_07940"
     CDS             complement(<1..1057)
                     /locus_tag="VLS86_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HSO50464.1"
                     /translation="MTEFGVHSEVGKLRKVMVHRPDLSLQRLTPSNHDDLLFDDVLWV
                     ERAQYEHDQFVAKMRSRGVEVFYFSELLSEALAASDEGRKRLIEVTVSEYTVGVSLVD
                     DVRALLDGMKPDQLAKHLIGGLTVHESGMRLEDARSWSLGAAALDDTSAFILPPLPNT
                     LFTRDSSCWIYGGVSINPMFWPARRLEAYNVATIYRYHPMFADADFEYWYPHLGDDGK
                     FQAADFGRSSLEGGDVEPIGNGTVLIGFSERTQARMIEQIARSLFDKGAADRVIAVVM
                     TKDRAHMHLDTVFTLLDRDKVTLYPKVMGSVRAISLRPGTAGKPFHVTEEDSFLGALG
                     DALGVDKVQVVETGGDAY"
     gene            complement(1089..2003)
                     /gene="arcC"
                     /locus_tag="VLS86_07945"
     CDS             complement(1089..2003)
                     /gene="arcC"
                     /locus_tag="VLS86_07945"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HSO50465.1"
                     /translation="MKVVVALGGNALLKRGEPMTADAQRQNVRTAAPSLAAVATEHAL
                     LLSHGNGPQVGLLALQAASYEEVDPYPLDVLGAQTEGMIGYVLEQELGNVLPFEVPLA
                     TLLTMIEVDPADPAFNNPTKFVGPIYDKARADAIAVEKGWTFKADGEHWRRVVPSPEP
                     KRIFEIRPIRWLLEKKTVVICAGGGGVPTMYDPEAERTLIGVEAVIDKDLASELLARE
                     VGADVFVMVTDVNGVYVGWGTPEQRRLEQTTPEELRALDFAAGSMGPKVEAAVRFVES
                     TGQRAVIGGLDQIVEIVAGSAGTQVVPG"
     gene            complement(2017..>2735)
                     /locus_tag="VLS86_07950"
     CDS             complement(2017..>2735)
                     /locus_tag="VLS86_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HSO50466.1"
                     /translation="NFATIVKAVEFGRAIYNNLFSYIRFQMSALVAFIVAYLLAAVFL
                     ILGGVPFTPLVVLWVNFLVQVPIAITLGFGKPVPGLMERKPRPLRQPILSRAQWVRVT
                     LMGLVMAVATVAVEAVYEDEGAVVAATMGFALFSVFNIVLGLTSRSETDSVFTRETVS
                     DSRQLLLVGIAFAFTILPTQLDFLQRWLGLTELTGYQWLIVLGLGMALVLVDEVVKIF
                     MRSRRHPLEMPPPMRPAIAV"
BASE COUNT          509 a          899 c          851 g          476 t
ORIGIN      
        1 gataggcgtc tcccccggtc tcgacgacct ggaccttgtc cacgccgagg gcatcgccga
       61 gggcgccgag gaagctgtct tcctcggtga cgtggaaggg cttgccggcg gtcccggggc
      121 ggaggctgat ggcccgcacg ctccccatga ccttggggta gagagtgacc ttgtcccggt
      181 ccagcaaggt gaagacggtg tcgaggtgca tgtgggcccg gtccttggtc atgaccaccg
      241 cgatcacccg gtccgctgcc cccttgtcga agagggagcg cgcgatctgc tcgatcatcc
      301 gggcctgggt ccgctcgctg aagccgatga gcacggtccc attcccgatc ggctccacgt
      361 cgccgccctc gagcgaggac cggccgaaat cggcggcctg gaacttcccg tcatccccga
      421 gatggggata ccaatactcg aaatccgcgt cggcgaacat cgggtggtac cggtagatgg
      481 tggcgacgtt gtatgcctcg agacggcgcg caggccagaa catcgggttg atcgaaactc
      541 ctccgtagat ccaacacgat gaatctcggg tgaacagcgt gttggggagc ggaggcagga
      601 tgaaggcgct ggtgtcgtcg agcgcggcgg cgcccaaaga ccacgaccgg gcgtcctcga
      661 ggcgcatgcc ggactcgtgc acggtcaggc cgccaatcag atgcttggcc aactggtctg
      721 gcttcatccc atccagcagc gcccgcacat cgtctaccag agacacaccc acggtgtatt
      781 ccgaaacagt gacctcgatc agccgcttgc ggccctcgtc gctcgccgcg agcgcctcgg
      841 agagcaactc ggagaagtag aagacctcca cgccacgact ccgcatcttg gccacgaatt
      901 ggtcgtgctc gtactgggcg cgctccaccc agagcacgtc atcgaagagg aggtcgtcgt
      961 gattggaggg ggtcagccgt tgcagactca ggtcaggccg atggaccatc accttgcgca
     1021 gcttcccgac ctcggagtga acaccgaact cagtcattct cgtctccttc ttggcacttg
     1081 gtcgcggctc aaccgggaac gacttgggtc cccgcgctgc ccgccactat ctccacgatt
     1141 tgatccaggc ctccgatcac cgcccgttgg ccggtgctct ccacgaacct caccgccgcc
     1201 tcgaccttgg gccccatcga cccggccgcg aaatcgagcg cccgcagctc ctcgggggtc
     1261 gtctgttcga gtcggcgctg ctcgggggtg ccccatccga cgtatacgcc gttgacatcg
     1321 gtcaccatga caaagacatc cgcccctacc tcgcgcgcca gcagctcgct ggcgaggtcc
     1381 ttgtcgatga ccgcttcgac tccgatcagg gttcgttcgg cctcggggtc gtacatggtg
     1441 gggacccctc cacccccggc gcagatgacc acggtcttct tctccagaag ccaccggatg
     1501 ggccggatct caaagatccg cttcggctcg ggcgagggca ccacgcggcg ccagtgctcc
     1561 ccatcggctt tgaatgtcca ccctttttcg acggcaatgg catcagcgcg ggccttgtcg
     1621 tagatcggtc ccacgaactt ggtgggattg ttgaaggccg ggtcggccgg gtccacctcg
     1681 atcatggtga ggagcgtggc caaaggcact tcgaaaggaa gcacgttgcc aagctcctgc
     1741 tccaagacgt acccgatcat gccctcggtc tgcgccccca ggacgtcgag cgggtagggg
     1801 tccacctcct cgtaggacgc ggcctgaagg gcgaggagac cgacctgtgg cccgttgccg
     1861 tgggagagga gcaaggcatg ctccgtcgcc accgccgcca gtgacggagc ggcggttcgc
     1921 acgttctgac gctgtgcgtc tgcggtcatc ggctcgccgc gcttcaacaa ggcattaccc
     1981 cccagagcca ccacgacttt catgatctca cctcgctcaa accgcgatcg ccgggcgcat
     2041 cggcggcggc atctccaacg gatgacgacg gctgcgcatg aagatcttga ctacctcgtc
     2101 gaccaggacc agagccatgc cgaggcccag aactatgagc cattggtatc cggtcagctc
     2161 ggtgagcccg agccatcgct gcaggaagtc gagttgggtc ggcagaatcg tgaacgcgaa
     2221 ggcgatccca accaggagca gctgtcggct gtccgagacg gtctcacgag tgaagacgga
     2281 gtcggtctcg ctgcgtgagg tcagtcccag gacgatgttg aacacgctga acagagcgaa
     2341 gcccatcgtg gcggcgacca ctgctccctc gtcctcatag accgcctcca cggccactgt
     2401 ggctacggcc atgaccagac ccatcagcgt gactctgacc cattgcgccc gtgacaggat
     2461 gggttggcga agcggccgcg gcttgcgctc catgaggccc ggaaccggct tgccaaatcc
     2521 cagggtgatg gcgatgggga cctggaccag gaagttcacc cacagcacta ccaaaggcgt
     2581 gaagggaacc cctccaagga tcaggaagac cgcggccagc agataggcaa ctatgaatgc
     2641 caccagcgcc gacatttgga accggatgta gctgaacaag ttgttgtaga tcgccctgcc
     2701 gaactcgacc gccttcacga tggtggcgaa gttgt
//
LOCUS       DATITW010000573         1903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_749386, whole genome shotgun sequence.
ACCESSION   DATITW010000573 DATITW010000000
VERSION     DATITW010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749386"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VLS86_07955"
     CDS             <1..1277
                     /locus_tag="VLS86_07955"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HSO50467.1"
                     /translation="VTASHNPPADNGYKVYGGNAAQIVPPTDQEIAVAIEKVGPADQV
                     PRIDGVFAGGSELVTPVVETIIDAYRIEVDEARPNPQTSNLRIVYTPLHGVGAKVLIR
                     MCAAAQHSGLITVPEQVAPDGRFPTLDFPNPEEPGALDLALALAEEEGADLVLANDPD
                     ADRLAVALPVEGGWRLLTGNELGALLGDYVLRYWGHPERPIVVNSIVSSPMMGRIAAQ
                     RGAVHETSLTGFKWIINAGLELESRGDGRFAFGYEEALGYSVGRTVRDKDGISAAIVM
                     ADLAAEETSADRSILARLHDLWDEFGLWVSAQHSIVRTGEEGKRALLAAVDRLARESP
                     TDVMGLTITGMTDYRVGEDRRPSWLGAQDLVELTIGDVGRILVRPSGTEPKLKIYVDL
                     SGTTEPNHTTAHRVLMEQATAVAAAMGEWLEI"
     gene            complement(1284..1463)
                     /locus_tag="VLS86_07960"
     CDS             complement(1284..1463)
                     /locus_tag="VLS86_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50468.1"
                     /translation="MFGPLNGVRVALVCFAVVAALAALIAGYPFVAAILLAGVAVHGL
                     GWLYLYRQHEREQSD"
     gene            1535..>1903
                     /locus_tag="VLS86_07965"
     CDS             1535..>1903
                     /locus_tag="VLS86_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50469.1"
                     /translation="MRSLLAAFLVLTGACTLDEARGTETTLVQDEVTIPPSTTGTPTT
                     LVTTTSAAITTMPAVEGPAKALVTPTGVVVAVLDETPAGFVVSSPCGNETLVTTGTPI
                     SSADVVIDPGHGGEIDTGAHG"
BASE COUNT          346 a          600 c          667 g          290 t
ORIGIN      
        1 tggtgactgc cagccacaac ccgccggccg acaacggata caaggtgtac ggggggaacg
       61 cggctcagat cgtgcccccc acagaccagg agatcgccgt ggcgatcgag aaggtggggc
      121 cggccgacca ggtgccgcgg atcgatgggg tcttcgccgg cggctccgaa ctggtcacgc
      181 ccgttgtcga gacgatcatc gatgcttacc ggatcgaagt cgacgaggcg cggcccaatc
      241 cgcagacgtc caatctgagg atcgtctaca ccccgctgca cggtgtcggt gccaaggtcc
      301 taatccggat gtgcgccgct gcccagcaca gcggtctgat caccgtcccg gagcaggtgg
      361 cgcccgatgg gagattcccg acgctggatt tccccaaccc cgaggagccg ggcgcactcg
      421 acctggccct ggctctggcg gaagaggaag gagccgatct ggtcctggcc aacgaccccg
      481 acgccgatcg cctggcggtg gccctgccgg tcgaaggtgg atggcggctc ttgaccggca
      541 atgagctcgg cgccctgttg ggtgactacg tgctcaggta ctgggggcac ccagagcgac
      601 cgatcgtcgt caattccatc gtgtcgagcc cgatgatggg tcggatcgcg gcacaacgag
      661 gggcggtgca cgagacgtcc ctgaccggct tcaagtggat catcaacgca ggtctggaac
      721 tggagtcgag aggcgatggc cgcttcgcat ttggctacga ggaggctctc ggctactcgg
      781 tggggcgcac cgtccgggac aaggatggga tctcggcggc gatcgtgatg gccgacctgg
      841 cagcggagga aacgtctgcc gatcgaagca tcctcgcccg cttgcacgac ctctgggacg
      901 agttcgggct ctgggtgagc gcgcagcact cgatcgtcag gaccggtgag gaggggaaac
      961 gtgccctgct ggccgcggtg gaccggctgg ccagggagtc cccgaccgac gtgatgggac
     1021 tgacgatcac cgggatgacc gactaccggg taggggagga cagacgaccg tcatggctgg
     1081 gggcacagga cctggtggag ctgactatcg gtgacgtggg tcggatattg gtcaggccga
     1141 gcggcaccga gccgaagctc aagatctatg tcgacctttc gggaacgacc gagccaaacc
     1201 acacgactgc acatcgagtc ctgatggagc aggcgaccgc cgtggcggct gcgatggggg
     1261 agtggctgga gatctgaccg ggctcagtcg gactgctcgc gctcgtgctg ccggtagagg
     1321 tagagccacc cgaggccgtg aaccgcgacg ccggccagca ggatggctgc gacgaacggg
     1381 tagccggcaa tcagggcggc gagggcagcg acgaccgcaa agcagaccag ggcgacccgc
     1441 accccgttca gaggaccgaa catcggccaa gcctatccgg cgcgctgtgt ttgttcgtcg
     1501 gcttgcaccg atgtcgggaa taaggtgacg cccgatgcgg tctctcctcg cggctttcct
     1561 ggtcctgacc ggggcctgca ccctcgacga agcccggggc acggagacaa cccttgtcca
     1621 ggatgaggtg acgatccctc ccagcaccac gggaaccccg accaccctgg tcacgacgac
     1681 atcggccgcg atcacgacga tgcctgccgt cgaggggccg gccaaggcgc tcgtcacccc
     1741 gaccggcgta gtggtggcag tgctcgacga gacgcctgcc ggtttcgtgg tgagctcccc
     1801 ctgcggcaac gagaccctgg tcacgaccgg cactccgatc agcagcgccg atgtggtcat
     1861 cgaccccggt catggtggtg agatcgacac cggcgcccac ggt
//
LOCUS       DATITW010000574         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_589540, whole genome shotgun sequence.
ACCESSION   DATITW010000574 DATITW010000000
VERSION     DATITW010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589540"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..833)
                     /locus_tag="VLS86_07970"
     CDS             complement(222..833)
                     /locus_tag="VLS86_07970"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HSO50470.1"
                     /translation="MMEIPKATVVRLPRYLRLLEDLESRETVSSEELAEAAGANAANV
                     RRDLSHLDFHGVRGVGYSVTDLRYRIRQELGIADRRQVAIIGAGNLGRALAAYGGLSR
                     RGFDVAAVYDTDTRKVGSKAGRVEVRNLQDMETDCRGGCFDMAILAVPAVAAQQVTDR
                     LVALGINSILNFAPVRVNVPANVTVRQVDLSMELQVLSYYGHR"
     gene            914..2152
                     /locus_tag="VLS86_07975"
     CDS             914..2152
                     /locus_tag="VLS86_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HSO50471.1"
                     /translation="MSSPSVLVIGGGPAGSAAAYWLARDGHEVTLVEKKEYPRDKTCG
                     DGLTPRAVLQLQEMGFDFEVPQFHTITGLRSYAGDDLMLEMNWPEHSRFPNWGGVMRR
                     SDLDSQVANLAAKQGVSILEKTEGFPIIEGGFLTGVSLKHNGSIEMVRPKVTVIADGS
                     MNRFGRELGTERRRDYPMGLAARGYYSSPRSTDPFLESQLDLRDSSGATMPGYGWVFP
                     LGDGTVNVGVGLLSTFTRWKHVNTTHMMADYAATAPAYWGLSEETKLTNPVGGKLTMS
                     FSKGPLVGSNWITIGDAAGAINPWNGEGISYAYETGRLAAEHIGDALTRDDLGLLRRY
                     PQHLDDEYGLYYKTARIFVKLIGHPSVMRTLAHTGLHNRTLMEWTLKVMSNLLDDDDK
                     HMGERIYDALAGVVRTLPTA"
BASE COUNT          390 a          705 c          693 g          401 t
ORIGIN      
        1 atcgaggggc tcaacgcggt ggagatccgg atcatcccgg tcacctatct gttggtttcc
       61 atggtgctgt tcctggtgtt cctggtggca ctacaccagt acgaggagtt gcaagagaaa
      121 tccagcgcgt agtctctccc cccagtgagc gaggcgagcg gtggggggag taccggcgga
      181 gccggggagg ggggcatctc cgacgcgtaa gtcggccggc attatcgatg gccgtaatag
      241 ctcaggacct ggagctccat cgatagatcc acctgtcgca ccgtgacgtt ggcggggacg
      301 ttgacccgca ccggggcgaa attgaggatc gagttgatcc cgagggccac caggcgatcg
      361 gtcacctgtt gcgccgccac cgcgggaacg gcgaggatcg ccatgtcgaa acaaccgccc
      421 cgacagtcgg tctccatgtc ctggaggttc cgcacttcga cccggcccgc tttggagccg
      481 acttttctgg tgtcggtgtc gtagacggcg gccacgtcga atcctcgacg gctcaacccg
      541 ccgtaggcgg ctagggcccg gcccaggttc ccggctccga tgatggcgac ctggcgccgg
      601 tcggcgattc ccagctcctg gcgtatccgg tatctgagat cggtcaccga gtagccgacc
      661 ccgcggacac cgtgaaagtc gagatgggag aggtcacggc ggacattggc ggcgttggcg
      721 ccggcagctt cggcaagctc ttcggagctg acggtctccc tggactcgag atcctcgagc
      781 aaccgcaaat agcggggcag tcggaccact gttgcctttg gtatctccat catccggggc
      841 gacgttatac cttctgcccc ggctctccgc ccgcctggtt cttggtcgcc gccaacctct
      901 aacctggcca ctcatgtctt ctccctcggt gctcgtgatt ggcggtggcc cggccggatc
      961 ggccgccgcg tattggctgg cacgggacgg acacgaggtc accctggtcg agaagaagga
     1021 gtacccccgg gacaaaacct gtggtgatgg gctgactccg agggcggtcc tccaactcca
     1081 ggagatgggc ttcgacttcg aagtgcccca gttccacacc atcaccgggc tccgctccta
     1141 cgcgggcgac gacctcatgt tggagatgaa ctggcccgag cattcccgtt tccccaactg
     1201 gggcggggtg atgcgacgca gcgacctcga ctcgcaggtg gccaacctcg ccgccaagca
     1261 gggggtttcc atcctggaga agaccgaagg gttccccatc atcgaggggg gcttcctcac
     1321 cggggtgtcg ttaaaacaca acgggtcgat cgagatggtc cggcccaagg tgaccgtgat
     1381 cgccgacggt tcgatgaacc gcttcgggcg ggagttgggc accgagcggc gccgtgacta
     1441 cccgatgggg cttgccgctc ggggttacta ctcctctcct cggtccaccg acccgttcct
     1501 ggaaagccaa ctcgatctac gcgactcctc gggtgcgacg atgcccggtt acggctgggt
     1561 attccctctc ggtgacggga cggtcaacgt cggcgtcggc ctgctctcca ctttcaccag
     1621 gtggaagcac gtcaacacca cccacatgat ggccgactac gccgccaccg ctcccgccta
     1681 ctggggcctg agcgaggaaa cgaagctgac caacccggtc ggcggcaagc tgacgatgtc
     1741 gttctcgaag gggcccttgg tcggctccaa ctggatcacg atcggcgacg cggccggtgc
     1801 gatcaacccc tggaacggcg aagggatctc atacgcatat gagacaggga ggctggccgc
     1861 cgagcacatc ggagacgccc tgacccggga cgatctcggg ttgctccggc gttatccgca
     1921 gcatctcgac gatgagtacg gcctctatta caagacggcg cgcatcttcg tgaagctgat
     1981 cggccacccc tcggtgatgc ggaccctggc ccataccggt ctccacaacc gcaccttgat
     2041 ggaatggacc ctcaaggtga tgtccaatct cctcgacgac gacgacaagc acatgggtga
     2101 gcgcatctac gacgccctgg ccggtgtggt tcggactctg ccgaccgcgt gaaacccaag
     2161 cttctcgtcc ttgacgtcaa cgagacgct
//
LOCUS       DATITW010000575         1900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_429226, whole genome shotgun sequence.
ACCESSION   DATITW010000575 DATITW010000000
VERSION     DATITW010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429226"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VLS86_07980"
     CDS             <1..276
                     /locus_tag="VLS86_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HSO50472.1"
                     /translation="PPANSRFCPDRRMTRAEFATFIVRALGLPPTDIDYFSDDNGHVL
                     EGAINRLAEAGITKGCNPPANDRFCPQSLLTRADTATFMTRAFRLTG"
     gene            complement(273..1229)
                     /gene="yedA"
                     /locus_tag="VLS86_07985"
     CDS             complement(273..1229)
                     /gene="yedA"
                     /locus_tag="VLS86_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HSO50473.1"
                     /translation="MSPEITAPSVKTTRAKVVAALIAVYFIWGSTYLGLRFGLESFPP
                     FLLNGFRFLLAGTILLLYLRLRGKATATRRQWWNAARMGVILLVGGVGMVTIAEDLGV
                     GSGIAATAVAVIPVWMALIGGLFGTWPHRREWIGLAIGLAGVLVLAQEGDFQASPIGM
                     ILIITSPIVWAFGSVWGARLDLPESSMATGIELATAGLVMTVVGPLFGERITAPPTTV
                     AWIALLYLAIMGSLVAFTAYIYLLKTVRPALSTSYAYVNPIVAVALGVSLGAEILTGP
                     VFIALPLILISVALVATTSVRRPSTTSDSPPHPAVEVREDAA"
     gene            1297..1854
                     /locus_tag="VLS86_07990"
     CDS             1297..1854
                     /locus_tag="VLS86_07990"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HSO50474.1"
                     /translation="MDFSHYGDQPVRTAVDLINSLDAVSGVDKLAGPTDLERFIASHD
                     GEWSRPDWRAGDRDLHEVRALRSRLRSVFESSNQAEAAEVLNSLLADVNAVPRVSVHG
                     PAPHLHFESVGDSPSRYLGAITAMGLSVALIEGGFDRFGICQSSTCADVFVDTSRNRS
                     RLHCSDTCTTRENVAAHRARQRHLG"
BASE COUNT          358 a          626 c          610 g          306 t
ORIGIN      
        1 ccgccggcga actcccggtt ctgcccggac cggaggatga ctcgcgccga gttcgccacg
       61 ttcatcgtca gggccttggg cctgcccccc acggacatcg actacttcag tgatgacaac
      121 gggcatgtgc tcgaaggtgc catcaaccgt ctcgccgagg ccggcatcac caagggctgc
      181 aatcctccgg ccaacgatcg cttctgtccc cagagcctcc tgaccagggc cgacaccgcg
      241 acattcatga cccgggcatt tcgactgacg ggctaagccg cgtcttctcg gacctctact
      301 gccgggtggg gcggggagtc gctggtggtc gacggccggc gcaccgaagt ggtggccacc
      361 agggcgacgc tgatgaggat cagggggagg gcgatgaaca ccggtccggt gagaatctcc
      421 gcaccgaggg agacccccaa cgccacggcc acgatcgggt tgacataggc atagctagtg
      481 gagagggcgg gacggaccgt cttcagcagg tagatgtagg cggtgaaggc caccagcgag
      541 cccatgatcg ccagatagag aagtgcgatc caggcgaccg tcgtcggagg tgcggtgatc
      601 cgctccccga acaagggtcc caccacagtc atcaccagac cggccgtggc cagttcgatg
      661 ccggtggcca tcgacgactc ggggaggtcg agtctcgccc cccagaccga cccgaacgcc
      721 cacacgatcg gggaggtgat gatgaggatc atcccgatcg ggctcgcctg gaaatctccc
      781 tcttgggcaa gcaccaggac gccggcgaga ccgatggcga gaccgatcca ctcgcgtcga
      841 tggggccagg ttccgaacag cccaccgatg agtgccatcc acaccggtat cacggccacc
      901 gcggtcgccg cgatccctga gccgacgccg aggtcctcgg caatggtgac catccccacc
      961 ccgccgacga ggaggatcac gcccatacgg gccgcgttcc accattgacg tcgtgtcgcc
     1021 gtggccttgc cccggagcct cagatagaga aggaggatcg tgccggcgag gaggaagcgg
     1081 aagccgttga ggaggaacgg cggaaagctc tccagcccga acctgagccc gagataggtc
     1141 gagccccata tgaagtagac cgcgatcagg gcggccacca ccttggctcg cgtggtcttg
     1201 acactcgggg cggtgatctc ggggctcatg acttccggta cggggctata atgtctttgg
     1261 accttacagg atagtcgcct cgaggtaagg gctcaaatgg atttctcgca ttacggagac
     1321 cagccggtgc gaacggctgt cgatctgatc aattcgctcg atgccgtgag cggagtggac
     1381 aagctggcgg ggccgaccga cctcgagcgc ttcattgcct cccatgacgg cgagtggtcg
     1441 cggcctgatt ggagagcggg ggaccgagac ctgcacgaag tccgtgctct tcgctcccga
     1501 ctgcgttcgg ttttcgagtc ctcgaaccaa gccgaggccg ccgaagtgtt gaacagtctc
     1561 ctcgccgacg tcaacgcagt gcccagggtg agcgtccacg ggccggcgcc acacctccat
     1621 ttcgaatccg tgggcgacag cccgtctcgc tatctggggg cgatcaccgc gatggggctc
     1681 agcgtggcgc tgatcgaagg cggcttcgac cgtttcggga tatgccagtc ctcgacctgc
     1741 gccgatgtct tcgtcgacac ctctcgaaac cggtcccgtc tccactgctc ggacacctgc
     1801 accacccggg agaacgttgc cgcccaccgg gcacgacagc gtcatctggg atgacgccgg
     1861 gaggcggcgc accactccaa tccggcaacc ctccccactc
//
LOCUS       DATITW010000576         1668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_709669, whole genome shotgun sequence.
ACCESSION   DATITW010000576 DATITW010000000
VERSION     DATITW010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709669"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VLS86_07995"
     CDS             <1..417
                     /locus_tag="VLS86_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50475.1"
                     /translation="GGFGDFNPHYVPIGIFGHDPEAPVYAQDLARAEELFRSVENPNG
                     GGSYWDTGFTVTVVAEGQNLFEIAAIVLKDSMTALNPLFDIQVAGVAEAEHQVRPVRQ
                     VRGPKQRGAPGPGPGASPRHSQTIRSKRADSRHADP"
     gene            404..1417
                     /locus_tag="VLS86_08000"
     CDS             404..1417
                     /locus_tag="VLS86_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO50476.1"
                     /translation="MQIRDYILRRLMVLPILLIGTSIIVFALTRVGGSPIGIYLSHEM
                     SQEEVAALEERFHLDDPLFVQYFFWISGVVQGDLGWSGVAAAPVTDVFPEKLAATMEL
                     ATCSALIAITLGLGLGTFAGARRNRLPDHIARVFSISGASMPLFWFAIVLLIVFWVYL
                     GWFPIGRSTPDIFARIAHPTGLYTIDSLLAGDLGAFRDAVWHLTLPALTLGYGATAII
                     ARMMRSSTVEELQEDYVEAARAKGLAERLVLKRHARRNALIPTVTVIGLSFGFLLQGS
                     VVAEIIFQWPGLGRWMADAVLRGDQATIMTFVMFTAVLFLFVNLAVDVVYAFLDRRVV
                     LGA"
     gene            1417..>1668
                     /locus_tag="VLS86_08005"
     CDS             1417..>1668
                     /locus_tag="VLS86_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50477.1"
                     /translation="MAEVHRLPLAPTHLDSAFRVRLRRFAELTGSVLSNPTTMVGAIL
                     IVLMIGMALLAPVLIDPNLPDPYQMPRDWSALNVPPGSPG"
BASE COUNT          258 a          562 c          531 g          317 t
ORIGIN      
        1 ggtggttttg gcgacttcaa cccacactat gtgccgattg ggatctttgg ccacgacccg
       61 gaggcgccgg tgtacgccca ggacctggcc cgagccgagg agctgttccg gtcagtggag
      121 aaccccaacg ggggcggctc ctactgggac accgggttca ccgtgaccgt ggtggcggag
      181 gggcagaacc tcttcgagat cgcggccata gtcctcaagg actcgatgac ggccctgaac
      241 ccgctgttcg acatccaggt tgccggggtg gccgaggccg aacatcaagt tcgccctgtt
      301 cgacaagtaa gagggcccaa gcaacgaggg gcgcccgggc ccggcccggg cgcctcgccg
      361 agacacagcc aaactatccg gtcgaagcga gcagactcga gacatgcaga tccgtgacta
      421 catcctgcgg aggctcatgg ttctgcccat cctgctcatc ggcacctcga tcatcgtctt
      481 tgccctgacc cgtgtcggtg ggtcgccgat cggcatctat ctctcccatg agatgagcca
      541 ggaagaggtg gcggcgctgg aggagaggtt ccatctcgac gacccgctct tcgtccagta
      601 cttcttctgg atctccggtg tcgtccaagg cgacctggga tggtccgggg tggccgcggc
      661 accggtcacg gacgtcttcc ccgagaagct cgcggcgacg atggagctgg ccacttgctc
      721 ggccctcatc gccatcaccc tcggtctcgg cctcgggaca tttgccgggg caaggaggaa
      781 ccgtctcccc gaccacatcg cccgggtgtt ctcgatcagc ggggcgagca tgccgctgtt
      841 ctggttcgcc atcgtcctcc tgatcgtgtt ctgggtgtac ctggggtggt tccccatcgg
      901 gcgcagcact cccgacatct tcgcccgcat cgcccatccc accgggctct acacgatcga
      961 ctcgttgctg gccggcgacc tcggcgcctt ccgtgacgcc gtgtggcacc tgacgctgcc
     1021 cgcgctcaca ctcggctacg gggcaacggc gatcatcgcc aggatgatga ggtcgagcac
     1081 ggtcgaagag ctccaggagg actacgtcga ggcagcccgg gccaagggcc tcgccgagcg
     1141 tctggtgctc aagcggcatg cccgtcgcaa tgcgctgatt cccacggtga cagtcatcgg
     1201 gctcagcttt ggcttcctcc tccagggaag cgtcgtcgcc gagatcatct tccagtggcc
     1261 gggactgggg cgatggatgg ccgacgccgt cctccgtggc gaccaggcca cgatcatgac
     1321 ctttgtgatg ttcaccgcgg tcctgttcct cttcgtcaat ctcgcggtcg acgtcgtcta
     1381 cgccttcctc gaccgacggg tcgtcctcgg ggcgtgatgg ccgaggttca ccgactcccg
     1441 ctggccccga cccacctcga ctcggcgttc cgggttcgcc tcaggagatt cgccgagttg
     1501 acgggcagcg tcctgtcgaa tccgacgacg atggtggggg cgatcctgat cgtgctcatg
     1561 atcggcatgg cgctcctggc gccggtgctg atcgacccca acctgcccga tccctatcag
     1621 atgccgcggg actggtcggc actcaatgtg ccgcccggct cgccgggt
//
LOCUS       DATITW010000577         2843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_69008, whole genome shotgun sequence.
ACCESSION   DATITW010000577 DATITW010000000
VERSION     DATITW010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69008"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VLS86_08010"
     CDS             complement(<1..273)
                     /locus_tag="VLS86_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020514824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSO50478.1"
                     /translation="MPNQPDRHLRATLPIPDRPAHGLTTYDAKDPDTSYSPIQPLLPP
                     EGAPNVLVILLDDVGFGSSSAFGGPINTPTAERLAAGGTRYTRFHTT"
     gene            complement(395..1684)
                     /locus_tag="VLS86_08015"
     CDS             complement(395..1684)
                     /locus_tag="VLS86_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSO50479.1"
                     /translation="MIALAILAAVFICYALVAARLDKRSITAPMVFVLVGALIGLAAP
                     EWLGPLGDPETVKHIAELTLALLLFADASTLRWRELREDGGLPIRLLLIGFPLTVLAG
                     FGAGIWLFPAAGWAAAALAASILAPTDAALGLGVFTNLSVPGRIRRALNVESGLNDGM
                     ATPLVTLFLAVLVAQEGTGPEDWVSESLRELGIGVLVALAVGAISGRLIRAAHDRGWT
                     SPLSERLAVLATALLSYGGAVTAGGNGFVAAFVAGIVFAVASRGKLHEPVEFTEDLAM
                     FASFLVWAIFGALLLAPQLRGGVSATAILYALLSLTLVRIVPVALAMVGTGLRPASLL
                     FMGWFGPRGLASVVFTLIAFETLVEGGLASDMMIEIATWTVFLSVIAHGVTAGPLATR
                     YGRSVSDEPIEQLEAGAHLGDPVPRALTESASPSTSP"
     gene            complement(1759..>2843)
                     /locus_tag="VLS86_08020"
     CDS             complement(1759..>2843)
                     /locus_tag="VLS86_08020"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HSO50480.1"
                     /translation="LVAALPYLQLPAVSPTSRHLVGKPKELMRALRANVAEVTGSEKP
                     EPVKLRRVTARSLIVSMLVLLIISALISAFTTIDFEAIWAVLQRANWSVIALALIVGH
                     TQFFPQATATMFAVPTRLPFWPLLTLQTASQFISLAIPSVAGRVAMNAAFLRKFGVSA
                     SAAVAQGAIDGFSGFLVQLAIMLIVLIGGDVDLNLDVATTDVQWLLVIAVIAALGIGV
                     VVAVLRIEALRRRVVPVVSQAWGALMVVFKQPIRALGLLGSNFVYWNVLGITLWLVLQ
                     SVGSDLSYGSALFAAAGTSLLAGFVPIPGGVGVAEATMTAILVTFGVDESTAIAVTAV
                     YRIVTFYLPALEGFFGARWLERNEYI"
BASE COUNT          585 a          880 c          929 g          449 t
ORIGIN      
        1 cgtggtgtga aagcgggtgt atcgggttcc cccggctgcg agccgctctg cggtgggggt
       61 gttgatcggg ccgccaaatg cgctggaaga gccgaagccg acgtcgtcga ggaggatcac
      121 gaggacattg ggcgccccct cgggagggag cagcggctgg atcggtgagt aactggtgtc
      181 ggggtccttt gcgtcgtacg tggtcaggcc atgagcgggt cggtccggaa tcggcaacgt
      241 ggcgcgcagg tgacggtcgg gttggttggg catactcatc tcctcgtggc atccctcatt
      301 gctacagcct caatccaacg gatgagcttg caccccggca tcgcctgatc aggatgataa
      361 ggatcgaagg tctcgggcgt ccgtcgctac ccgattacgg gctcgttgat ggcgacgcgg
      421 attcggtgag cgctcggggg acgggatcgc cgagatgagc tccggcttcg agttgctcta
      481 ttggttcatc tgaaacactc ctcccgtacc tggtagccag cggcccggcg gtcaccccgt
      541 gggcgatgac cgagaggaag acggtccagg tggcgatttc gatcatcatg tctgaggcga
      601 gccctccctc gaccagtgtt tcgaacgcaa tcaaggtgaa cacgacggat gccaatccgc
      661 ggggaccgaa ccaccccatg aataagaggc tggccggtcg cagacccgtc ccgaccatcg
      721 ccagggccac gggcacgatc cgcaccaggg tgaggctcag caaggcgtag agaatcgctg
      781 tggctgacac cccgcccctc agttgaggcg caaggagcaa ggccccgaag attgcccaga
      841 ccaggaacga ggcgaacata gcgaggtcct cggtgaactc gactggctcg tggagctttc
      901 ctctcgacgc gacagcgaaa acaatgccgg cgacgaaggc ggcgacgaag ccgttaccgc
      961 cagccgtcac cgcgccgccg taggagagca gcgcggtcgc cagaacggcg aggcgctctg
     1021 agagcggaga cgtccaaccc cgatcatggg cggctcggat gagccgtccc gagatggctc
     1081 ctacggcgag agcgactaag acaccaatcc cgagttcacg cagggactcg ctcacccagt
     1141 cttcaggacc ggtgccctcc tgagcgacga gtacggcgag gaaaagggtc accaacggcg
     1201 tcgccatccc atcgttcaga ccgctctcga cattgagtgc acggcggatt cgccccggaa
     1261 ccgacaggtt ggtgaagact cccaacccga gtgcggcgtc cgtgggggca aggatgctgg
     1321 ccgccagcgc tgccgccgcc catccggcgg cggggaacag ccatatgccg gccccgaatc
     1381 cggcgagcac ggtgagtggg aacccgatta ggagcagacg gatcggaagg ccaccgtcct
     1441 cgcgcagctc acgccaccgg agtgtggagg catcggcgaa gagcaggaga gcgagggtca
     1501 gctcggcgat gtgcttcacc gtttccgggt cgcccaacgg tccgagccat tcgggagccg
     1561 cgagcccgat gagggcaccg acgagaacga agaccattgg cgcggtgatc gaccgcttgt
     1621 cgagacgggc ggcgactagg gcgtagcaga tgaacaccgc ggccaggatt gccagcgcga
     1681 tcatgagcag atccttcctc gcaggtggga ttcgttgcgg tcgttcaagg tgacgtcgac
     1741 actttctcga tctcctgtct agatgtactc gttccgctcc aaccagcggg caccgaagaa
     1801 cccctccagg gcgggcagat agaaagtaac gatccggtag acggcggtga cggcgatcgc
     1861 ggtcgactcg tcgacaccaa aggtgaccaa gatcgcggtc atcgtcgcct cggccacacc
     1921 gactccaccc ggaatcggca cgaatccggc gagaaggctg gtgccggctg cggcaaagag
     1981 ggccgagccg taactcaagt ccgagccgac ggattgcaaa acgagccaaa gggtgatccc
     2041 gagcacgttc caatagacga agttgctgcc caagaggcct aaggctcgga tcggctgttt
     2101 gaagaccacc atcagtgctc cccaggcctg gctcactact ggaacgactc tcctgcgcag
     2161 tgcttcgatc cgcagaaccg cgacgaccac tcctatccca agggccgcga tcaccgcgat
     2221 caccaatagc cactgcacat cggtggtcgc gacgtcgagg ttcaagtcga catctcctcc
     2281 gatcaacacg atgagcatga tcgccaactg gaccaggaac ccagagaatc cgtcgatggc
     2341 gccctgcgcc accgccgcag aggccgagac cccgaacttg cggagaaatg cggcgttcat
     2401 cgccacacgg cccgccacgc tcgggatcgc caggctgatg aactggctcg cggtctgcag
     2461 ggtcaacaac ggccagaaag ggagcctcgt gggcaccgca aacatggttg ctgtggcctg
     2521 agggaagaac tgagtgtgcc ccacgatcag agcgagcgcg atgacggacc agttcgccct
     2581 ctggaggaca gcccagatcg cctcgaaatc gatagtggtg aatgccgaga tcagggccga
     2641 gatgatcagg agcaccagca ttgacacgat caggcttcgt gcggtcaccc ggcgcaactt
     2701 gaccggctcg ggcttctctg acccggtgac ttcggccacg tttgcgcgca gggccctcat
     2761 gagctccttc ggcttcccta ccaggtggcg gctcgttggg ctcactgcag gtagttgcag
     2821 gtagggcagg gcagcgacca aac
//
LOCUS       DATITW010000578         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_148573, whole genome shotgun sequence.
ACCESSION   DATITW010000578 DATITW010000000
VERSION     DATITW010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148573"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VLS86_08025"
     CDS             complement(<1..445)
                     /locus_tag="VLS86_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HSO50481.1"
                     /translation="MSLELRYPDGQVVQHPDGVTGLQVAESIGPRLAKSAVAVKLNGE
                     LRDLNRALDSSGDFAVITLATDEGLDVLRHSSAHVLAQAVLDLFDDATFAIGPAIEDG
                     FYYDFKVEEPFTPDDLDRIEKRMGEIIREDQPFEREAVSAEEALRV"
     gene            580..1365
                     /locus_tag="VLS86_08030"
     CDS             580..1365
                     /locus_tag="VLS86_08030"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HSO50482.1"
                     /translation="MVRELSSGANKVFRIIDGDQSSIVKVYPVSSRERRERHALEALA
                     VVPAVPRIAGHGTEGEMAWLRMNDAGHWSLASLPKNLEAVEASGAALRGVHSSKAQIT
                     NLAAGIDSDYVLSHYRSTIERLGRYRRRFGMEQVVLDKALQLEPPLATAPAPSHTKPV
                     LSKFVVDEDGSITIVDWEWATLAPPEWDLTLAVWRLAARIGEDAAAAFRSGYGAELPE
                     SRYRPWVAYHAAMMMLEAAEVREGRLGDLSYLVDDLTEAVIGT"
     gene            1437..1646
                     /locus_tag="VLS86_08035"
     CDS             1437..1646
                     /locus_tag="VLS86_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50483.1"
                     /translation="MLEALVAATATTGLFALAKGLRGNHSYKVDPSLVVTPRGERGDD
                     LPCPWCYGPTTEEDARCRGCGVAFG"
     gene            complement(1647..1901)
                     /locus_tag="VLS86_08040"
     CDS             complement(1647..1901)
                     /locus_tag="VLS86_08040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSO50484.1"
                     /translation="MGFCWTKAHADGVGEIYLLGVDPAHRGRGVGRVLAEHGVRHLLE
                     DRECPEVIAFWDEENRAATNLFESIGFGVDRIDEVYRLEL"
     gene            1791..>2116
                     /locus_tag="VLS86_08045"
     CDS             1791..>2116
                     /locus_tag="VLS86_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50485.1"
                     /translation="MLCENATNSATPVRRVYPQQVDLANAVGMGLRPAKADEMRLRSF
                     QEQVSPIETWLGESDLEIRPVPRPTCRLPGDRQPEQTHELLFVDIPSKRADGRVGAAR
                     GEAATG"
BASE COUNT          388 a          686 c          687 g          355 t
ORIGIN      
        1 agacgcgaag cgcctcctcc gccgagaccg cctccctctc gaagggctgg tcctcgcgga
       61 tgatctcccc catccgcttc tcgatgcggt cgagatcgtc cggtgtgaac ggctcttcga
      121 ccttgaagtc gtagtagaag ccgtcctcga tcgcgggccc gatggcaaat gtcgcatcgt
      181 cgaacaggtc gaggacagcc tgggccagga catgggccga tgagtggcgc aacacgtcga
      241 gaccctcgtc cgtggccagg gtgatcacgg cgaaatcgcc actgctgtcg agggcccggt
      301 tgagatcgcg caactcccca ttcagcttga cggcgaccgc ggatttggcg agacggggcc
      361 cgatcgactc ggcgacctgg agcccggtga cgccatctgg gtgctggacg acttgtccgt
      421 ctggataacg aagctctagt gacacggggt tatctcgcta gcctgggggc gggcaagagg
      481 ctaactgcat gcagaacgca agcgtgtcgg ccgttggcgc acaaggggat caccagcatg
      541 gatgcaagag agcttgtcac gaagcttcac ccgaactcaa tggtacggga gctttcctcc
      601 ggcgcgaaca aggtctttcg gatcatcgac ggggaccaga gctcgatcgt gaaggtctat
      661 ccggtttcct cacgagaacg acgtgagcgg cacgccctgg aggcgctggc cgtggtgccg
      721 gccgttcccc ggatcgccgg tcatggcacc gagggggaga tggcctggct gcgaatgaac
      781 gacgccggtc attggagtct cgccagcctg cccaagaacc tggaggccgt ggaggcatcc
      841 ggggccgcgc tgcgcggggt ccattcgtcg aaggcccaga tcacgaatct ggcggcgggc
      901 atcgactcgg actacgtcct cagccactac cggtccacca tcgagcgtct cggccggtac
      961 cggcgaagat tcggcatgga acaggtggtc ctggacaagg cactccaact ggagccgccg
     1021 ctggccactg ccccggcgcc gtcgcacacc aagccggtcc tgtccaagtt cgtcgtcgac
     1081 gaagacggct cgatcacgat cgtcgactgg gagtgggcca ccctcgcccc gccggagtgg
     1141 gatctgaccc tggccgtgtg gcgcctcgcg gcgcgcatcg gcgaggatgc cgcggccgcc
     1201 ttccgcagtg gatacggagc ggagcttccc gagagccgct accgcccctg ggtggcgtat
     1261 cacgcggcga tgatgatgct ggaggcggcc gaagtccggg agggccgtct cggcgatctg
     1321 agctacctgg tcgacgacct gaccgaagcc gtcatcggca cctgacccct gcgaaatcca
     1381 cggatttcgc ccccctccgc cttagcctca ttgacgctga acaaggagga cgggcagtgt
     1441 tggaagctct ggttgcagca accgccacga cgggtctgtt cgctctggcc aagggattgc
     1501 ggggcaatca ctcatacaag gtggatccga gtctggtggt gactccacgg ggggaacgtg
     1561 gcgatgacct gccttgcccc tggtgctacg ggccgacgac ggaggaagac gcccgctgca
     1621 ggggatgtgg cgtcgccttc ggctgatcac agctccaggc gatagacctc atcgatccgg
     1681 tcgacaccga agccaatcga ctcaaagaga ttcgtcgccg cacgattctc ctcatcccag
     1741 aaggcgatga cctcagggca ttcccggtcc tcgagcagat gcctcacccc atgctctgcg
     1801 agaacgcgac caactccgcg accccggtgc gcagggtcta cccccagcag gtagatctcg
     1861 ccaacgccgt cggcatgggc cttcgtccag caaaagccga tgagatgaga ctcaggtctt
     1921 tccaggaaca ggtctcgccc atcgaaacct ggctgggaga atctgacctc gagatccgcc
     1981 cggtcccacg ccccacttgc aggctgcccg gtgatcgtca gccggagcag actcacgagc
     2041 tcctcttcgt cgacatcccg tcgaaacgtg ccgatggccg tgtcggggcg gcgcggggcg
     2101 aagccgccac cggtga
//
LOCUS       DATITW010000579         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_109125, whole genome shotgun sequence.
ACCESSION   DATITW010000579 DATITW010000000
VERSION     DATITW010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109125"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VLS86_08050"
     CDS             <1..842
                     /locus_tag="VLS86_08050"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HSO50486.1"
                     /translation="TDQAMSVVSSPGIDVVVEAIGGLEPTGELLLIALRSGKPVVTAN
                     KELVATRGEELFEAADRSGVPLMFEAAVGGGIPVIRPLAESLAGEQIDRVSGIVNGTT
                     NYILTSMTQDGRSFEEALEEATALGYAEPDPSDDVSGRDAAFKTAILARLAFGEWVAP
                     WQVDCEGIEAVSRADIEAAALTGKVIKLVGVAERVGKSVSTRVSPIALDKDHPLAGVD
                     GVTNAIFVEGPAIGRLLFTGPGAGGQPTASALLGDLIQVASRRNWQRKAPSYRRPRQV
                     LHR"
     gene            complement(831..1148)
                     /locus_tag="VLS86_08055"
     CDS             complement(831..1148)
                     /locus_tag="VLS86_08055"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HSO50487.1"
                     /translation="MSFSDELAGRVRARMSHLGGLTERPVMSGLGFFLDDRMAVVILD
                     DRLCLRIPDGDKGDFDRMSARPFEFAGRPVRGWVCIPEASLDEASLSGWVARGVSGVG
                     SAM"
     gene            complement(1158..1628)
                     /locus_tag="VLS86_08060"
     CDS             complement(1158..1628)
                     /locus_tag="VLS86_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HSO50488.1"
                     /translation="MKPRHGPAIMAAMRDGQVRRMSRLHRWLYKTTRGAVGKRLVDND
                     ILLLTTTGRRTGRPHTVPLLYLREGETVVLIASYGGRDRHPAWYLNLAATPEVEVQLR
                     QQQFKARARTADPEERAAWWPRVVAAYGDYAVYQTRTDRVIPVVLLEPLREGAT"
BASE COUNT          305 a          526 c          527 g          271 t
ORIGIN      
        1 tcacagacca ggcaatgagc gtggtgagca gtccgggcat cgacgtcgtt gtggaagcga
       61 tcggtggtct ggagccgaca ggcgagctcc tcctcatcgc gttgagatcg ggcaagccgg
      121 tggtcaccgc caacaaagaa ctggtggcga cccggggcga ggagctgttc gaagcggccg
      181 atcgaagtgg ggttcccctc atgttcgaag cggcagtggg cggggggatt ccggtgatcc
      241 gaccgctggc cgagagtctg gcgggggagc aaatcgaccg ggtgagtggg atcgtcaacg
      301 gcaccaccaa ctacatcctg acttcgatga cccaggacgg ccgaagcttc gaggaggccc
      361 tggaggaggc caccgccctc ggatatgccg agccggaccc ctccgacgac gtgtcgggga
      421 gagatgccgc cttcaagaca gcgattctcg ctcggctcgc cttcggagag tgggtcgcgc
      481 cctggcaagt ggactgtgag ggaatcgaag ccgtgagccg agccgacatc gaggccgccg
      541 cactcaccgg gaaggtgatc aagttggtcg gagtcgcgga acgggttggc aagagtgtgt
      601 caacacgggt atcaccgatt gccttggaca aggaccatcc attggcaggc gtcgatggcg
      661 tgaccaatgc gatcttcgtg gaaggccccg ccatcgggcg tctcctcttc accggtcccg
      721 gggcgggcgg tcaacccacc gcctctgccc tgcttgggga cctcatccag gtcgcttccc
      781 ggcgcaactg gcagcgcaag gctcccagtt atcgccgccc gcgacaggtg ctacatcgct
      841 gacccaacgc cggacacccc acgtgccacc caacccgaca gactggcctc gtcgaggctc
      901 gcttcgggta tgcacaccca gcctctgacc ggacgcccgg cgaactcgaa tgggcgagct
      961 gacattcggt cgaagtcgcc cttgtcgccg tcggggatac ggagacacag ccgatcgtca
     1021 agaattacaa ccgccatcct gtcgtcgaga aagaacccga gcccggacat gaccggccgc
     1081 tcggtcaaac ctcccagatg ggacatccgg gctcgaaccc ggccggccag ctcatcggaa
     1141 aaggacacct tccgaatcta ggtggcaccc tcgcggagtg gctccaacag caccaccggg
     1201 atcacccggt cggttcgtgt ctgatacacc gcatagtcgc cataggcggc gacgacccgt
     1261 ggccaccagg ccgcacgctc ctcgggatca gcggtgcggg ctcgggcctt gaattgctgt
     1321 tggcgcagct ggacctcgac ttccggtgtc gccgccagat tgaggtacca ggcggggtga
     1381 cggtccctgc ccccatagga ggcgatcagc accacggttt ccccctcccg cagatagagc
     1441 agcgggaccg tgtgaggacg accagtccgt cggccggtcg tggtgagaag gagtatgtcg
     1501 ttgtcgacca atcgcttccc cactgcaccc ctggtcgtct tgtagagcca gcggtgcaac
     1561 cgtgacatgc ggcgcacttg accatccctc attgcggcca ttatcgctgg accgtgcctg
     1621 ggtttcacc
//
LOCUS       DATITW010000580         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_469143, whole genome shotgun sequence.
ACCESSION   DATITW010000580 DATITW010000000
VERSION     DATITW010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469143"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VLS86_08065"
     CDS             <1..798
                     /locus_tag="VLS86_08065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSO50489.1"
                     /translation="REVVRLSEEAHRIKNDFVAMVSHELRTPLTSIAGFADTLVDSWK
                     DLPEEEVDEFLGIINRQSVYLGDLVEDVLVIPRLEADRLRFHPELFDLGELIDDVAKM
                     VFPNGGKKSSLVSLPDGVRVKADRRRVQQVIRNLMENARKYGGDQIMVEGFVMGDQYL
                     IVISDNGPGVPDDETQRVFDNFEQLSKGDTREATGIGLGLPIARRLARAMGGEVWYER
                     RFPTGARFCYSLPLRRRSVVGDDEPMEPVELDPTAAVELGDQVASAT"
     gene            846..>2794
                     /locus_tag="VLS86_08070"
     CDS             846..>2794
                     /locus_tag="VLS86_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSO50490.1"
                     /translation="MQAEWFFDDVPADSTLFADLNPTQREAVAATEGPVLVVAGAGSG
                     KTRVLTYRIAHLLRDLRVPPQSVLAITFTNKAANEMKSRVGALVGGAVRSMWVSTFHS
                     ACVRILRREASRLGYRSGFSIYDDADALRLLNLVLKDLDLDSKRFPPKAMKAVISKAK
                     NELVDFESFAEQGDGFYHEQVSDIYRLYQQRLLEASAMDFDDLLMVTVELFGAFPEVL
                     AGYQERFRYVLVDEYQDTNRAQYMLVRQLTASHGNLCVVGDSDQSIYKFRGADIRNIR
                     DFETDYPEARIVLLDQNYRSTETILQAANSVISNNTKRTPKNLWSDRGRGVPILRYEA
                     EDEHDEAGYVVDEVERLQAGGFNLSDLAVFYRTNAMSRVIEDVFVRRGLPYTVVGSVK
                     FYDRKEIKDAVAYLRAVVNPADEVSLKRIINEPRRGIGDTTIAHIDRFSQSHKIPFAD
                     ALGRVSEINMLSARAQTAVREFVAIMGRVRDAVEGAGVAAGVESVLIDTGLVPTLESE
                     QTIEAMGRVENLRELAGVASEFEASNEGAVIGDEEFDTLDNMRRLEVFLESTALVADI
                     DEWDEGAGAVTLMTLHTAKGLEFPAVFIVGMEDGVFPHMRSLGDPAELEEERRLAYVG
                     ITRAQDKLYLTSAWSRMLFGGSNYNP"
BASE COUNT          491 a          884 c          927 g          492 t
ORIGIN      
        1 cgcgaggtcg tgcgtctctc cgaggaggcg catcggatca agaacgactt cgtggcgatg
       61 gtcagccacg agttgaggac accactcacc tcgatcgccg ggtttgccga cacgctggtc
      121 gattcctgga aggacctccc cgaggaggag gtggatgagt tcctggggat catcaaccgc
      181 caatcggtgt atctcggtga tctggtcgag gatgtgctgg tcatcccccg tctcgaggcc
      241 gaccggctcc gctttcaccc cgagttgttc gatctcggcg agctgatcga cgacgtggcc
      301 aagatggtgt tccccaacgg cgggaagaag agctcgctgg tgtcgctccc ggacggggtc
      361 agggtgaagg ccgaccgtcg gcgtgtgcag caggtcatcc gcaacctgat ggagaacgcc
      421 cgcaagtacg ggggcgacca gataatggtc gagggcttcg tcatgggcga ccagtacctg
      481 atcgtgatct cggacaacgg ccccggagtc cccgacgacg agacccagcg ggtgttcgac
      541 aacttcgagc agctctccaa gggagacacc cgggaggcga ccggcatcgg gctcggcttg
      601 ccgatcgccc gtcggctggc ccgagcgatg ggcggcgagg tctggtacga gcgcaggttc
      661 cccaccggtg cccgcttctg ttactccctg cccctgcgtc ggaggtctgt ggtcggcgac
      721 gacgagccga tggaaccggt cgagctcgat ccaacggcgg ccgtggagct cggcgatcag
      781 gtcgcttccg ccacctgatc acactgttgt gattcagcct ggcggcgcca gtagcatcga
      841 gccagatgca ggctgagtgg ttcttcgatg acgtaccggc cgactccacc ctgtttgccg
      901 acctcaatcc gacacagcgc gaggcggtgg cggcgaccga aggcccggtg ttggtggttg
      961 ccggtgccgg ctcgggcaag acccgggtcc tgacctatcg catagcccac ttgcttcgcg
     1021 acctgcgggt gccgccccag tcggtgctcg ccatcacttt caccaacaag gccgcgaacg
     1081 agatgaaatc ccgggtgggg gcgctggtcg ggggagcggt gcgctcgatg tgggtctcca
     1141 cctttcactc cgcctgtgtt cgcatcctgc gtcgcgaggc gtcacggctc ggctatcgct
     1201 ccggcttctc gatctacgac gacgccgacg ccttgcgtct gctcaacctg gtcctgaaag
     1261 acctcgacct ggactcgaag cgctttccac ccaaggcgat gaaggcggtg atctccaagg
     1321 ccaagaacga gctcgtcgac ttcgagtcgt tcgccgagca gggcgacggc ttctatcacg
     1381 agcaggtctc ggacatctac cggctctatc agcagcggct cctcgaggcc tcggccatgg
     1441 acttcgacga cctgttgatg gtgaccgtgg agttgttcgg agctttcccc gaggtgctcg
     1501 ccggctatca ggagcgtttc cgctacgtcc tggtcgacga gtatcaggac accaaccggg
     1561 cccagtacat gctggtgaga cagctcaccg cgagtcacgg caacctgtgt gtggtcggag
     1621 actccgatca gtcgatctac aagtttcgcg gggcggacat ccgcaacatc cgggacttcg
     1681 agacggacta cccggaagcc cgaatcgtgc tcctcgacca gaactaccgc tcgacggaga
     1741 ccatcctcca ggccgccaac tcggtcatct ccaacaacac caagcggacc ccgaagaacc
     1801 tgtggtccga ccgggggcgc ggggtgccca tcctccgcta cgaggccgag gacgagcacg
     1861 acgaggccgg ctacgtcgtc gacgaggtcg agcgtctcca ggccggcggg ttcaatctct
     1921 ccgacctggc cgtcttctat cggaccaatg ccatgtcccg cgtgatcgag gacgtcttcg
     1981 tccggcgcgg gcttccctac accgtggtgg gctcggtcaa gttctacgac cgcaaggaga
     2041 tcaaggacgc ggtcgcctat ctgagggcgg tggtcaaccc ggccgacgag gtctcgctga
     2101 agcggatcat caacgagccg cggcggggaa tcggagacac caccatcgcc cacatcgacc
     2161 gtttcagcca gagccacaag atcccgttcg ccgacgccct gggcagggtg tccgagatca
     2221 acatgctcag cgcccgggcc cagacggctg tccgcgagtt cgtggcgatc atgggacggg
     2281 tgagagacgc cgtcgagggc gccggggtgg cggccggggt ggaatcggtc ctgatcgaca
     2341 ccgggctggt gccgaccctg gagtccgagc agacgatcga ggcgatgggg cgggtggaga
     2401 acctgcgcga gctggccggg gtggcctccg agttcgaggc gtcgaacgag ggtgcggtca
     2461 tcggtgacga ggagttcgac accctcgaca acatgagacg tctcgaggtc ttcctcgagt
     2521 ccaccgctct ggtggctgac atcgacgagt gggacgaggg tgcgggagcg gtgaccttga
     2581 tgaccttgca caccgcaaag ggcctcgagt tcccggcggt gttcatcgtc gggatggaag
     2641 acggggtctt tccccacatg cgctccctcg gcgacccggc agagctggaa gaggagcggc
     2701 gcctggcata cgtggggatc acccgtgccc aggacaagct gtatctcacc tcggcctgga
     2761 gccgcatgct gtttggtggc tccaactaca accc
//
LOCUS       DATITW010000581         1715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_109172, whole genome shotgun sequence.
ACCESSION   DATITW010000581 DATITW010000000
VERSION     DATITW010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109172"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VLS86_08075"
     CDS             complement(<1..612)
                     /locus_tag="VLS86_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50491.1"
                     /translation="MRRWTIALSLVFVVIVVVLFIVLTGDQAGETTTTVFGASSITTA
                     PGSTTTGEGTTTTNPETTTTSLETTTTSSAVGLEGNWAEEPLVVASFGALGWWDGAGW
                     VQVDEGTSLPVAGGEDYQIAVLGVDSITSGGGEELLCDPLNNPGVVLADDEMLGDWPG
                     PYGVAISADWVLTPHFVAETTDDGTYAAVASGLLAERGLTVPNP"
     gene            complement(622..1215)
                     /locus_tag="VLS86_08080"
     CDS             complement(622..1215)
                     /locus_tag="VLS86_08080"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HSO50492.1"
                     /translation="MRLVLASGSPRRRALLETIGLDFDVEPSEVDETRLPDEAPAAYV
                     ERVARSKATSAAGPGRLVIAADTTVVHEGRVMGKPGHPEEARAMLRRLQGDTHEVLTG
                     LAVASWDEAPVVHTVVDVSMVEMVAMTEDEIADYVDTGEPMDKAGAYAVQGMGGVFVH
                     RVIGSPFTVIGLPIHLLDRLMAAAGAGLSHFRIPASR"
     gene            complement(1212..>1715)
                     /locus_tag="VLS86_08085"
     CDS             complement(1212..>1715)
                     /locus_tag="VLS86_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50493.1"
                     /translation="MAREVPSMAVRAGVHSIEHGLFLTEADVIELGARGGIWVPTVLR
                     MEAVIRQLGADSSGGRLIREGIENVASLLDTAVEDGVFLEAGTDLAVGTRAVAAEAVR
                     LWELGMSPVRVVDAVSTAGLLATGRTGGFEFGSPANVVLYPENPVTDPRVLAHPKQVV
                     RLGVPLG"
BASE COUNT          306 a          602 c          543 g          264 t
ORIGIN      
        1 cgggttgggc acggtaagcc cacgttcggc gaggagcccc gacgctactg cggcgtacgt
       61 cccgtcatcg gtggtctctg cgacgaagtg aggcgtcaag acccaatcgg cggatatcgc
      121 aacgccatag ggcccgggcc aatcacccag catctcgtcg tcggcgagga cgacgccggg
      181 attgttcaac gggtcgcaca acagctcctc accgcctccg ctggtaatgg aatccacccc
      241 aagaacggcg atctggtaat cctcgccacc ggccaccgga agactcgttc cttcatcgac
      301 ctggacccag ccggcgccgt cccaccatcc cagggcgccg aaactggcga caacgagcgg
      361 ctcctccgcc cagttgccct ccaagccgac ggcgctggag gtggtggtgg tctccaagga
      421 ggtcgtggtg gtctccgggt tggtggtggt ggtcccctca ccggtcgtgg tcgagcccgg
      481 cgcggtggta atgctcgacg ccccgaatac cgtggtcgtg gtctcaccgg cctggtcacc
      541 ggtgagcacg atgaagagca cgaccacgat caccacgaac acgagggaaa gggcgatggt
      601 ccaacgacgc atcagcccaa cctacctgct ggccgggatt cggaagtggg agagcccggc
      661 gcctgcggcc gccatcaagc gatcgaggag atgaatggga agccctatga cggtgaacgg
      721 gctcccgatc actcggtgca cgaagacacc acccatgccc tgtactgcat aggcgcctgc
      781 cttgtccatc ggctcgccgg tgtcgacgta gtcggcgatc tcgtcctctg tcatcgcgac
      841 catctcgacc atcgagacgt cgacgactgt gtgcaccaca ggagcctcgt cccacgacgc
      901 cacggccaat ccggtgagaa cctcgtgggt atcgccctgg aggcgtcgca gcatggcccg
      961 agcctcttcg ggatggcccg gctttcccat gaccctgccc tcgtgaacga cggtggtgtc
     1021 ggccgcgatc accaggcgcc cgggaccggc ggctgaggtg gccttggaac gggccacccg
     1081 ctcgacatat gccgccggtg cctcgtcggg cagacgggtc tcgtcgacct ctgacggctc
     1141 cacgtcgaaa tcgagaccga tcgtctcgag cagcgcccgt cgccgtggag agcccgaagc
     1201 caggaccagt ctcatcccag gggcactccc aaacgaacca cctgcttcgg gtgggcaagg
     1261 acacgaggat cggtgaccgg gttctcgggg tagagcacga cattggccgg gctcccgaac
     1321 tcgaagccac cggtgcggcc ggtggccagg agccccgcgg tggagacggc gtccacgacc
     1381 cggactgggc tcatccccaa ctcccaaagt cggactgcct cagccgccac cgctcgggtc
     1441 cccaccgcca gatcggttcc cgcctcgagg aagaccccgt cctccaccgc ggtgtcgagc
     1501 agcgacgcca cgttctcgat gccttcgcgg atcaaccggc ctccactcga gtcggcgccg
     1561 agctgacgga tgaccgcctc catgcggaga accgtgggga cccagatccc gccccgggcc
     1621 cccaattcga tcacgtcggc ttcggtgagg aagaggccgt gctcaatcga gtggacaccg
     1681 gctcggaccg ccatcgacgg cacctctcgg gccat
//
LOCUS       DATITW010000582         1686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_709836, whole genome shotgun sequence.
ACCESSION   DATITW010000582 DATITW010000000
VERSION     DATITW010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709836"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1686
                     /locus_tag="VLS86_08090"
     CDS             <1..>1686
                     /locus_tag="VLS86_08090"
                     /inference="COORDINATES: protein motif:HMM:NF036968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HSO50494.1"
                     /translation="YRAKLVLRDVAKTFGFTQAQVDGLSKYVDTRDPGQLRLDTPLPE
                     GMTAEMIYDICWRLDGYPRHLGIHSGGMVIADRPLWQVVPLEWGRLEGRTVLQWDKDD
                     SAAVGIVKFDLLGLGMLNALHLAHDLIEDQHGVDVDLAAIPQEQAIYRSITRSDTVGL
                     FQIESRAQMATLPKMKPKTFYDLAVEVALIRPGPIQGQSVHPYLRRRNGEETVRYPHP
                     LAEPILKKTLGVPIFQEQLMELARICAGFNGGQADRLRQAMTHKRSETAMGKLRDEVY
                     AGMAGNGIVGAAADEIWEKLQGFASFGFPESHSVSFAYIVYMSAWLRFHYPAEYLAGL
                     LNAQPMGFYSPNSLVQDSQRHGVVVMGPDVNLSWHDCTIEPIETDPEDIVEYLGERWR
                     RGRGVLDDPIRPAVGVRMGLRYVRNLGDKEVTRVEAARILGGEFASPEDLASRTGLGV
                     EALEGLAASGALESVGLGRREGMWAAGALAEIDPERLALSPGVEAPSLPGMDAEEEHR
                     ADLWSTGVSNRHPMSFVRDQLDGCLTAAQALAMERNGSRIKVAGVVTHRQRPG"
BASE COUNT          274 a          541 c          597 g          274 t
ORIGIN      
        1 cctatcgggc caagttggtg ctgcgggacg tggccaagac cttcgggttc acccaggccc
       61 aggtggacgg gctctccaag tacgtggaca cccgggaccc gggacagttg cgtctcgaca
      121 ccccgctccc ggaggggatg accgctgaga tgatctacga catctgctgg cgactggacg
      181 ggtacccgcg gcatctcggc atccattccg gggggatggt gatcgccgac cgccccctgt
      241 ggcaggtggt ccccctggag tgggggcggc tggaggggcg gaccgtcctg cagtgggaca
      301 aggacgactc cgcggcggtg gggatcgtca agttcgacct gctcggtctg gggatgctca
      361 acgctctcca tctcgcccac gacctgatcg aagatcagca cggggtcgac gtcgacctgg
      421 ccgccatccc ccaggagcag gccatctacc gctcgatcac ccgctccgac acggtgggcc
      481 tgttccagat cgagtcgcga gcccagatgg cgaccctgcc caagatgaag ccgaagacct
      541 tctacgacct ggcggttgag gtggctctca tccgccccgg gccaatccag ggtcagtcgg
      601 tccaccccta tctgaggcga cggaacgggg aggagacggt tcgctacccc caccccttgg
      661 cggagccgat cctgaagaag accctcggcg ttcccatctt ccaggagcag ctgatggagc
      721 tggcccggat ctgcgccggg ttcaacggag gtcaggcgga tcgacttcgt caggcgatga
      781 cccacaagcg ctcggagacg gcgatgggaa agctccggga cgaggtgtac gccggcatgg
      841 ccggcaacgg gatcgtcgga gcggccgccg acgagatctg ggagaagctc cagggctttg
      901 ccagctttgg cttcccggag agccactcgg tgagcttcgc ctacatcgtc tacatgtctg
      961 cctggctccg attccactat ccggcggagt acctggccgg tctgctcaac gcccagccga
     1021 tgggcttcta cagcccgaac tccctggtcc aggactcaca gcgtcatggg gtggtggtaa
     1081 tgggccccga cgtgaacctc tcctggcacg actgcaccat cgagccgatc gagaccgacc
     1141 ccgaagacat cgtcgagtac ctgggggaga ggtggcgtcg ggggagaggt gtcctcgatg
     1201 atccgatccg ccctgcggtg ggggtgcgga tgggtctgcg ttatgtgagg aacctgggag
     1261 acaaggaagt gacccgggtc gaggcggcgc ggatcctggg tggggagttc gcctcgcccg
     1321 aggacctggc ctcgcgcacc gggctcgggg tggaggccct ggaaggcctg gccgcctccg
     1381 gtgccctgga gtcggtcggt ctcgggcgtc gcgaggggat gtgggcggcc ggcgccctcg
     1441 ccgagatcga ccccgagcgt ctcgccctct ctccgggggt ggaggcgccg tcgcttccgg
     1501 ggatggacgc cgaggaagag catcgtgccg acctgtggtc gaccggggtc tccaaccggc
     1561 acccgatgag ctttgtccgc gaccaactcg acgggtgtct caccgcggct caggcgctgg
     1621 ccatggagag aaacggctcc cggatcaagg tggcgggggt ggtcacccac cggcagcggc
     1681 ccggca
//
LOCUS       DATITW010000583         3014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_589761, whole genome shotgun sequence.
ACCESSION   DATITW010000583 DATITW010000000
VERSION     DATITW010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589761"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VLS86_08095"
     CDS             complement(<1..740)
                     /locus_tag="VLS86_08095"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HSO50495.1"
                     /translation="MAFPDGIADFRSDTVTRPTPRMLEAMVSAPLGDDVYSDDPTVNA
                     LEEESAAVTGKEAAIFVPTGTMGNQLAIMAQTHPGEEVLAHEGSHVRSIEAGAPQALS
                     GVGFRTVSGDGGRIEPEDVEQAMSMAGFFPRIGLMVWENTHNLSGGRVIPIDVMEKTS
                     QVARSLGLRIHVDGARIFNAVVASGVPAARYAAIGDTIQFCFSKGLGAPVGSIVCGAA
                     DLIAEIRYLRKRLGGGMRQAGVIAAAARV"
     gene            complement(750..1925)
                     /locus_tag="VLS86_08100"
     CDS             complement(750..1925)
                     /locus_tag="VLS86_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSO50496.1"
                     /translation="MIDFRRIGSLPPYAFAEVNRRKTEARLAGVDVIDLGFGNPDIPS
                     PDFAVDSLIEAARDPANHRYSASRGIDDLRLALAGRYQRRFGVELDPETEVISTIGSK
                     EGLSHLMWALLQPGDAAIVPEPSYPIHLYAPIIAGAEVRSAPIGSEEDYFDVVHRMFS
                     ESWPRPRVIIVSFPNNPTTKAVDIGFFERLVGFARENEVVIVHDFAYADIAFEGYRPP
                     SILEVPGAKEVAVEIYSLTKGHSMAGWRVGFMAGNSEVVAALAKLKSYLDYGTFQPIQ
                     IAAAAALNQGDGFVEEVNEIYRARRDTLVDGLADVGWKMERPRATMFTWAPVPGPYED
                     LDSTEFSIELLDRAGVAVSPGVGFGQSGEGFVRFALDEDERRIETAVERIGDTLRRL"
     gene            complement(2042..2470)
                     /locus_tag="VLS86_08105"
     CDS             complement(2042..2470)
                     /locus_tag="VLS86_08105"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HSO50497.1"
                     /translation="MTENTKRVKPDDVIPPDGESIQGRQMVRDAALMLPNLVKLVGRL
                     LVDPRVPRRAKVVLGFAAAYVASPIDLIPEFIPVLGWADDLLLLTYALDSLIDRAGPE
                     VVEEHWDGPGDLLDLIRDVMGMGRNLVPRPLMRTIDRLSG"
     gene            complement(2467..2652)
                     /locus_tag="VLS86_08110"
     CDS             complement(2467..2652)
                     /locus_tag="VLS86_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50498.1"
                     /translation="MREQSEAMQALGRAGRRVVYHLLQAAIEGLKAVEAVIEEVGNIG
                     DKDDDDIDNTRGRIEIE"
     gene            complement(2649..>3014)
                     /locus_tag="VLS86_08115"
     CDS             complement(2649..>3014)
                     /locus_tag="VLS86_08115"
                     /inference="COORDINATES: protein motif:HMM:NF037143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50499.1"
                     /translation="AFADVPRLHLRLFDDEMVGIDRLRFMAGELYGDRDPILDFTAKH
                     PFRIIDNDGVIAMELDIPFIDKSEFDVYRHGHELYVSVGPYRRSFILPDALHRRDVGR
                     ARLEGGTLTVVFSDPDVKK"
BASE COUNT          524 a          992 c          981 g          517 t
ORIGIN      
        1 acccgggccg cagccgcgat cacacccgcc tgacgcatcc cgccgccgag tcgcttcctg
       61 aggtatctga tctcggcgat cagatcggcg gcgccgcaca cgatggagcc gaccggcgcc
      121 ccgagaccct tggagaagca gaactggatc gtgtcgccga tcgccgcgta acgggcggcc
      181 gggactccgc tggccaccac cgcgttgaag atccgggccc cgtcgacgtg gattctgaga
      241 ccgagggacc gggcgacttg ggacgtcttc tccatgacgt cgatcgggat cacccggccg
      301 ccggacaggt tgtgggtgtt ctcccagacc atgagaccga tcctggggaa gaagcctgcc
      361 atcgacatcg cctgctcgac atcctccggc tcgatcctcc ccccgtcgcc gctcaccgtc
      421 ctgaagccga ccccggacag cgcctgggga gcgccggcct cgatgctgcg gacgtggctc
      481 ccctcatgag ccagcacctc ctcgccggga tgggtctggg ccatgatcgc caactggttt
      541 cccatggtcc cggtgggcac gaagatcgcg gcctccttgc ccgtcacggc cgccgattcc
      601 tcctccagcg cgttcaccgt ggggtcgtcc gagtagacgt cgtcgcccag gggggccgag
      661 accatcgcct ccagcatccg gggcgtgggt cgggtgactg tgtcggagcg aaagtcggcg
      721 atgccgtcgg gaaaggccat gtccgaaggc tacagacgcc tcagcgtgtc accgatccgt
      781 tccaccgcgg tttcgatcct tcgttcgtct tcgtccaggg caaaacgcac gaaaccctcg
      841 ccactctgcc caaagccgac tccggggctg accgcgacac ctgccctgtc gagcagctcg
      901 atcgagaact cggtcgagtc gagatcctca tagggaccag ggactggcgc ccaggtgaac
      961 atcgtggccc gcggccgttc catcttccac ccaacatcgg cgagaccgtc gaccagggtg
     1021 tcgcggcggg cgcggtagat ctcgttgacc tcttcgacga agccgtctcc ctggttgagg
     1081 gcggccgcgg ctgcgatctg gatcggctgg aaggtcccgt agtcgagata gctcttgagc
     1141 ttggccaggg cggccaccac ctcggagttg cccgccatga acccgacccg ccagcccgcc
     1201 atggagtggc ctttggtcag cgagtagatc tcaaccgcca cctccttggc tcccggcacc
     1261 tccaggatcg acggtggtcg gtatccctcg aaggcgatgt cggcgtaggc gaaatcgtgg
     1321 acgatgacga cttcgttctc cctggcgaag ccgaccagcc gctcgaagaa gcctatgtcc
     1381 acggctttgg tggtggggtt gttggggaaa ctgacgatga tcacccgggg acggggccag
     1441 gattcgctga acatgcggtg gacgacgtcg aagtagtcct cctcggagcc gatcggggcc
     1501 gacctcacct cggccccggc gatgatgggg gcgtagagat ggatcggata ggaaggctcg
     1561 gggacgatgg ccgcgtcgcc gggttggagc agcgcccaca tcaggtggga caggccctcc
     1621 ttcgacccga tggtcgagat cacctcggtc tccgggtcga gctcgacgcc aaaccgccgc
     1681 tgatagcgcc cggcgagagc cagccgcagg tcatcgatgc cccgcgatgc cgagtagcga
     1741 tggttcgccg ggtcccgagc ggcctcgatc agggagtcca ccgcgaagtc aggcgacggg
     1801 atgtcggggt tgccgaaccc gaggtcgatg acgtcgactc ccgccagccg cgcctcggtc
     1861 ttgcgccggt tgacctcagc gaaggcataa gggggaagcg aaccgatgcg tctgaagtcg
     1921 atcacgggcg ggtcaagtta acccggagac gggcaaagtc cgagccaccc ctccgtaatt
     1981 gggacgggga ggggtcatcc ggacctgtgg ctcccaattt cgggaagggg ggctccggat
     2041 gtcagccgga gagacggtcg atggtccgca tcagcgggcg ggggaccaga ttgcgtccca
     2101 tgcccatcac gtcgcgtatc aggtcgagga ggtcgccggg gccgtcccag tgctcctcca
     2161 ccacctcggg gccggcacgg tcgatcaacg agtcgagggc gtaggtcagc agcagcaggt
     2221 cgtccgccca cccgagcacc gggatgaact cggggatcag gtcgatgggg ctggcaacgt
     2281 aggcagcggc aaagccgagc accactttgg cccgccgcgg caccctgggg tcgacgagca
     2341 gccttcccac caacttgacc aggttgggaa gcatcaaggc ggcatcccgg accatctggc
     2401 gtccttgaat cgactccccg tcggggggaa tcacgtcatc tggcttgacc cgcttcgtgt
     2461 tctcggtcat tcgatctcga tcctgcctcg tgtgttatcg atgtcgtcgt catccttgtc
     2521 gccgatgttc cctacctctt cgatcacagc ctccaccgcc ttgaggccct cgatggccgc
     2581 ttggagaagg tgatagacca cccggcgccc ggcgcggcca agagcctgca tcgcctcgga
     2641 ttgctctctc acttcttcac gtccgggtcg gagaagacaa ccgtcaatgt cccgccttcg
     2701 agacgagccc gtcccacatc gcgacggtga agcgcgtcgg ggaggatgaa cgatcgacga
     2761 taggggccga cgctcacgta gagctcgtgc ccatggcgat acacgtcgaa ttccgacttg
     2821 tcgatgaacg gtatgtcgag ctccatggca atcaccccgt cgttgtcgat gatccggaac
     2881 ggatgcttgg ccgtgaaatc taggatcggg tctctgtccc cgtacagctc accggccatg
     2941 aagcgcagcc ggtcgattcc gaccatctcg tcgtcgaaga gccgaagatg gagacggggc
     3001 acgtcggcga aggc
//
LOCUS       DATITW010000584         1745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_389079, whole genome shotgun sequence.
ACCESSION   DATITW010000584 DATITW010000000
VERSION     DATITW010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389079"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..601
                     /locus_tag="VLS86_08120"
     CDS             44..601
                     /locus_tag="VLS86_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HSO50500.1"
                     /translation="MRVLVAYASRHGATVGIAERIADGLIATGFEAEARSVKEVDDIA
                     PYQAFVVGSAAYMHHWLKEATSFVTRHRAMLSESPVWLFSSGPVGTDLVDAEGRDIFE
                     ASRPVEFDELKTMVNPRQERVFFGAWDPDAEPVGFAERFAKPFLRLAPKDALPTGDFR
                     DWAAIDQWAAEIAAELWAGDRTLTV"
     gene            complement(699..1082)
                     /locus_tag="VLS86_08125"
     CDS             complement(699..1082)
                     /locus_tag="VLS86_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50501.1"
                     /translation="MRDGWIQQNALVRRVVVLGLMMLVACNSDVSPQRAFCDQAVPLL
                     SQEDMGQEPEEAVISQMEELARIAEVLPEEQQGPLMVLIEDLNEQLRLWGQGRSEDGW
                     SSLDVVEYVGAQCLRDDLTWWMVTP"
     gene            complement(1086..>1745)
                     /locus_tag="VLS86_08130"
     CDS             complement(1086..>1745)
                     /locus_tag="VLS86_08130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HSO50502.1"
                     /translation="GKRERDARRGFTLPPKLGISTIFDGIVHPGWTWQFLTHEPIVFA
                     NVVGREVGYGAEAVSLAEYVGSQFDPGLSWNDVEWLRSRWSGPIVLKGIQTVADAIIA
                     ADEGVEAIALSNHGGRQLDSAPAPLDLLPSVAEAVGGRTEIICDGGVRRGSDIVKALA
                     LGATACMAGRAYLYGLGAAGEAGVDHVLSLLSSDMKRTMALIGCPSIADLNPDYLSIA
                     R"
BASE COUNT          313 a          574 c          555 g          303 t
ORIGIN      
        1 cgtccggtct aaccatgcgg gtgaccgaaa aaaggagcaa tccatgagag ttctcgtggc
       61 ctacgccagc cggcacggtg ccaccgtggg gatcgcggag aggatcgccg acgggctgat
      121 tgcgacgggg ttcgaggcgg aagcccgatc cgtgaaggaa gtcgatgaca tcgccccata
      181 tcaagcgttc gtcgttggca gcgctgcgta catgcatcat tggctgaagg aggccacttc
      241 gttcgtcacc cgccatcggg cgatgctttc cgagagcccc gtgtggctgt tcagcagcgg
      301 tccggtcggg accgacctgg tcgacgccga gggccgcgac atcttcgagg cctcccggcc
      361 ggtggagttc gatgagctga agaccatggt caacccacgc caggagcgtg tcttctttgg
      421 agcctgggat cccgatgccg agcctgtggg cttcgcggag cgttttgcca agcccttcct
      481 tcgactcgcc cccaaggatg cgcttcccac gggtgacttc cgggattggg cggcgatcga
      541 ccagtgggcc gcggagatag cggcggagct ctgggcgggc gatcggacgt tgaccgtctg
      601 aggtccaccc ctcgtcacga aggaacctcg ctggggccga ttggccgcga cccgggtaga
      661 aagataagga actgagaccc tgatggccgt ctccgaatct acggcgtgac catccaccac
      721 gtcaggtcgt cacggagaca ttgcgccccg acgtattcga cgacatcgag actggaccaa
      781 ccgtcctcgg aacggccctg cccccacaac cggagctgct cgttcagatc ctcgatcagg
      841 accatcaacg gtccctgctg ttcctcgggc agtacttcgg cgatgcgtgc cagttcctcc
      901 atctgactga taacggcttc ttctggctcc tgccccatgt cctcctggga gaggagtggc
      961 accgcttggt cgcagaacgc ccgctggggt gacacatccg aattgcacgc cacgagcatc
     1021 atcaagccca ggaccacgac gcgtcgcaca agggcattct gctggatcca gccgtcccgc
     1081 aactgctaac gggctatgga taggtaatcc ggattgaggt ccgcgatgga cgggcacccg
     1141 atgagggcca tcgttcgctt catgtccgat gacaacaggc tcagcacgtg gtcgaccccg
     1201 gcttccccgg cagcgcccag tccgtacagg taggcgcgac cggccatgca ggcagttgcc
     1261 cccagggcca gcgccttgac gatgtcgctg cccctgcgca cgccgccgtc gcagatgatc
     1321 tcggtgcgac cgcccaccgc ctcggcaacc gaaggcagca agtcgagtgg ggcgggagcg
     1381 gagtcgagtt ggcggccgcc gtgattggag agggcgatgg cctccacgcc ctcgtcggcg
     1441 gcgatgatcg cgtcggcgac ggtctggatc cccttgagca cgatcgggcc gctccatcga
     1501 ctgcgaagcc actccacgtc attccacgac agacccgggt cgaactggct gccaacgtat
     1561 tcggccagag acaccgcttc tgccccgtag cccacctccc gaccgacgac attggcgaac
     1621 acgatcggct cgtgcgttag gaactgccat gtccaacccg ggtggacgat cccgtcgaag
     1681 atggtcgaga tcccgagctt gggggggagg gtgaagccac gcctcgcgtc gcgctcccgc
     1741 ttccc
//
LOCUS       DATITW010000585         1842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_509234, whole genome shotgun sequence.
ACCESSION   DATITW010000585 DATITW010000000
VERSION     DATITW010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509234"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VLS86_08135"
     CDS             <1..391
                     /locus_tag="VLS86_08135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSO50503.1"
                     /translation="DRMVAPRVDGDQVSYLGPVGAEDRGGLLGDARALLHLISFEEPF
                     GFSVVEAMACGTPVIAYPRGSMPEIIRHGEGGFLVESVEEAVAAVDASANIDRSGVRA
                     SVETRFDADRMVDEYLAVYHQVLAGPR"
     gene            complement(393..>1842)
                     /locus_tag="VLS86_08140"
     CDS             complement(393..>1842)
                     /locus_tag="VLS86_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HSO50504.1"
                     /translation="HASARFTPDPTRVIAKPFLPGEQIFPDGQSRTHRVVKRVLAMSD
                     AEVTTTLASFFEDFGDRHLGLEGALERHFAVVSHYVDDPDSLGPDRRLLIGAYFTHEY
                     SIEASALGNPSMVPAPDQTGLQPGERRFIISLRAIGEGHLSSIEFRSGVIDAGSEITI
                     DEPSGFATTGSRRSAVYEKRLFRAKLAEMGVLNEIAAMVLDLLPERFSIDEINSAIAE
                     VSQAVDRPIAFETAKTIHWLASSNYELTFDQGSTVSERVIFPAGPTESRGMEDARFVQ
                     FIGDDGSIIYYATYTAFDGFQILPQLIETSDLVSFEIATLQGSGARNKGVALFPRLID
                     GRYAALSRQDNENNYLMLADDVRLWDEAEKIQVPTRPWELMQLGNCGSPMETEAGWLV
                     ITHGVGPMRRYSLGAILLDAADPRRVIGHLHDPLLVPSEDERDGYVPNVVYSCGGMIH
                     GENLILPYGYSDYGARIAKVRLDDLLTELTSR"
BASE COUNT          340 a          520 c          648 g          334 t
ORIGIN      
        1 cgaccggatg gtggcgccac gggtggatgg agaccaggtc agctatctgg ggccggtcgg
       61 cgccgaggat cggggagggc tgttgggcga tgcccgcgcc ctgctccatc tcatcagctt
      121 cgaggagccg tttgggttca gcgtggtcga ggcgatggca tgcgggacgc cggtgatcgc
      181 ctatccacga ggttcgatgc ccgagatcat caggcatggt gaaggcggat tcctggtgga
      241 gtcggtggag gaggcggtag cagcggtgga cgcttcggcg aacatcgatc gctccggggt
      301 ccgggcttcg gtcgaaacca ggttcgacgc cgatcggatg gttgacgagt acctggcggt
      361 gtatcaccag gtgttggccg gcccccgctg aatcagcgag acgtcagctc ggtgagaagg
      421 tcgtcgaggc gcacctttgc gatccgggcc ccgtaatcgg agtagccata ggggaggatc
      481 aggttctcgc cgtgaatcat gcccccgcag gaatagacca cgttggggac gtaaccgtca
      541 cgctcgtcct cggatggaac cagcagcgga tcatgcaggt ggccgatcac ccggcgcggg
      601 tcggcggcat cgagaaggat ggcccccagg gaatagcggc gcatcggccc caccccatgg
      661 gtgatgacca gccacccggc ctcggtctcc atcggtgaac cgcagttgcc caactgcatc
      721 agctcccagg gccgggtcgg gacctggatc ttctccgcct cgtcccagag ccggacatcg
      781 tcggcgagca tcaaatagtt gttctcgttg tcctggcggg aaagggcggc gtagcgaccg
      841 tcgatcaggc gggggaacaa agcgacaccc ttgttgcggg caccgcttcc ctggagcgtg
      901 gcgatctcaa acgagacgag atccgaggtc tcgatgagtt gggggaggat ctgaaaaccg
      961 tcgaatgcgg tgtaggtggc gtagtagatg atcgatccgt cgtctccgat gaattggacg
     1021 aagcgggcgt cctccatccc gcggctctcg gtgggccctg cggggaagat cacccgttcg
     1081 gagacggttg atccctggtc gaaggtcagc tcgtaattcg acgaggcgag ccagtggatg
     1141 gtcttggcgg tctcgaaggc gattgggcgg tcgaccgctt ggctgacttc agcgatggca
     1201 gagttgatct catcgataga gaatcgctcg ggcaggagat cgaggaccat ggccgcgatc
     1261 tcattgagca cgcccatttc ggccagcttg gcccggaaca gccgcttctc gtagacggcc
     1321 gagcggcgac tcccggtggt ggcgaagcca ctgggctcat cgatcgtgat ctcggatccg
     1381 gcatcgatga cccccgatcg gaactcgatg gacgagagat gtccttctcc gatcgcccgg
     1441 aggctgatga tgaagcgtct ttcgccaggt tggagaccgg tttggtcggg ggcgggcacc
     1501 atggacggat tacccaatgc ggaagcctcg atcgagtact cgtgtgtgaa gtaggcgccg
     1561 atcagaagtc ggcggtcggg cccaagactg tcggggtcgt cgacataatg ggagaccacg
     1621 gcgaaatgac gctccagggc cccctccaga ccgaggtggc ggtctccaaa atcctcgaag
     1681 aaggaggcca acgtggttgt cacctcggca tccgacatcg ccaacacccg cttgaccacc
     1741 cggtgggtgc gcgattggcc gtcggggaag atctgttctc cggggaggaa gggcttggct
     1801 atgacccgag tcgggtccgg ggtgaagcga gcactcgcat ga
//
LOCUS       DATITW010000586         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_349041, whole genome shotgun sequence.
ACCESSION   DATITW010000586 DATITW010000000
VERSION     DATITW010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349041"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VLS86_08145"
     CDS             complement(<1..414)
                     /locus_tag="VLS86_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50505.1"
                     /translation="MLCQSCYSLCPGRICARCLAGLRPAAERILEGGVRLIAGFEHVG
                     AARELVHAFKYRGFTAYADLVVEMIGPRLPVLPIVPVPRAWTRQVLHGIDPARELGRR
                     LAKYRGVPLCEVLVAPVHSRRRAGGDHSRPPPRFAT"
     gene            complement(479..1729)
                     /gene="ahcY"
                     /locus_tag="VLS86_08150"
     CDS             complement(479..1729)
                     /gene="ahcY"
                     /locus_tag="VLS86_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HSO50506.1"
                     /translation="MNYDIANPALAGDGARRVEWAGRRMQVLATVRERFSKERPLEGL
                     VVAACLHVTAETANLMLALRDGGAEPILCASNPLSTQDDVAAALVAEGIPVFAIRGED
                     ADTYYKHIHALLDHAPAITMDDGADLATILHTDRRDIEVIGSTEETTTGVIRLKAMSA
                     DGTLRVPVVAVNDSATKHLFDNHYGTGQSAIDGIIRATNVLLAGLNFVIIGYGDCGRG
                     VARRADGMGAKVVVVEVDPVRALAAAMDGFQVMTASDAARVGDVFVTVTGNKHVLRKE
                     HFDVMKDGAILANAGHFDIELDLASLRADAVANRQIRKDLEEFEMADGRRILLAAEGR
                     LVNLGAAEGHPADVMDMSFSNQALAAEYLAMNAGKLEPKIYTLPTEIDAEVARIKLEQ
                     MGGGLEVLTDDQKAYLSGWQEGTA"
     gene            complement(1731..>2009)
                     /locus_tag="VLS86_08155"
     CDS             complement(1731..>2009)
                     /locus_tag="VLS86_08155"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HSO50507.1"
                     /translation="PEVTRELLGIVTLTDRSDHPRIRARLDHSASLTEFAVPWLAEIP
                     ATGESELARLMSLTVSGDLVSWMLAEEAGVDPVPVATIEKLKKLLVED"
BASE COUNT          346 a          693 c          633 g          337 t
ORIGIN      
        1 ggtcgcgaat cgaggcgggg gacgggaatg gtctccaccg gccctacgcc ggctgtggac
       61 gggggccacc aggacttcgc acagcggaac accccggtat ttggccagtc gccgtcccag
      121 ctcacgagcg ggatcgatcc cgtgtaacac ttgccgggtc catgccctgg ggacgggcac
      181 tatcggcagc acggggaggc gggggccaat catctccacc accaggtcgg catacgcggt
      241 gaagcctcga tacttgaagg cgtgaaccaa ctcgcgggcg gcaccaacat gctcgaaccc
      301 cgcgatcagc cggaccccgc cctccaggat tctctctgct gccggtctga gaccagccag
      361 gcatctggcg cagatgcgcc ccgggcaaag cgagtagcac gactggcaca acacctctca
      421 gaacgtatcc ccgaccgggg acaaaaccgt cacccgccgt cgcgggcggc acccatgctc
      481 aggcagtccc ctcctgccaa cccgacaggt acgccttctg gtcatcggtg aggacctcga
      541 gaccgccacc catctgctcc agcttgatcc gggccacctc ggcgtcgatc tcggtgggca
      601 gggtgtagat cttgggctcc agcttgccgg cgttcatcgc caggtactcc gcggccaggg
      661 cctgattgga gaaggacatg tccatcacgt cggcgggatg gccctccgcc gcgcccaggt
      721 tcaccagtct tccttcggca gcgagcagga ttcgccggcc gtcggccatc tcgaactcct
      781 ccaggtcctt gcggatctgg cggttggcca cggcatcggc gcgtagcgat gccagatcca
      841 gctcgatgtc gaagtgcccg gcgttggcca ggatcgcacc gtccttcatc acatcgaagt
      901 gctccttgcg caggacgtgc ttgttcccgg tgacggtcac gaagacatcg cccacccttg
      961 ccgcgtccga agccgtcatc acctggaagc cgtccattgc ggccgccagt gcccggacgg
     1021 ggtccacctc gaccacgacc accttggccc ccatcccgtc ggcacgccgc gccacgccac
     1081 gaccacagtc gccgtagccg atgatcacga agttgaggcc tgcgagcagg acgttggtag
     1141 ctctgatgat gccgtcgatg gccgactgcc ccgtgccgta atggttgtcg aacaggtgct
     1201 tggtcgccga gtcgttgacc gcgaccaccg ggacccgaag cgtcccgtca gccgacatcg
     1261 ccttgagacg gataacgccg gtcgtggtct cctcggtcga acctatgacc tcgatgtctc
     1321 gccggtcggt gtggaggatg gtggcgagat ccgccccatc atccatggtt atcgccggcg
     1381 catgatcgag aagggcgtgg atgtgcttgt agtaggtgtc ggcgtcctcg ccccggatgg
     1441 cgaacaccgg aattccttcg gccaccagtg cggcagcgac atcgtcctga gtggacagcg
     1501 gattcgaagc acagaggatc ggctcggcgc ccccgtcacg caatgccagc atgaggttgg
     1561 cggtctcggc ggtgacatgg agacaggcgg cgaccacgag gccctcgagg ggccgctcct
     1621 tgctgaaccg ctcgcgcacg gtggccagca cctgcatccg ccgcccggcc cactcgaccc
     1681 gtcgcgcacc gtctccggcg agggccgggt tggcaatgtc gtaattcata tcaatcctct
     1741 actaggagct tcttcagttt ctcgatggtg gcgaccggga ccggatcgac gccggcctcc
     1801 tcggccaaca tccacgacac gaggtctccg ctgaccgtga gcgacatcag ccgggccagc
     1861 tccgactccc ccgtcgcggg aatctcggcc agccagggca ccgcgaactc ggtcagggaa
     1921 gccgaatgat cgagccgggc cctgatccgc gggtgatccg accgatcggt gagggtcacg
     1981 atcccgagca gttcgcgggt gacctctgg
//
LOCUS       DATITW010000587         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_29165, whole genome shotgun sequence.
ACCESSION   DATITW010000587 DATITW010000000
VERSION     DATITW010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29165"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VLS86_08160"
     CDS             <1..628
                     /locus_tag="VLS86_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HSO50508.1"
                     /translation="ILRMLGAEVWETPDDLCPVDLPKDGAIALARSLAASEQGERYVM
                     SDQYANPDNVTAHYETTGPEIWRQTEGRIGWFVTGLGTGGTITGVGRFLKERDPSIRV
                     IGIEPQPGHRLPGLKSFQEAKQPEILDWDVVDDVIRVDDGPAYDTTRRLWREEGLTVG
                     PSTGAVVDAIQRLETTSDDVVVGISADSGFKYTSYFAEILGDEGLPRV"
     gene            643..912
                     /locus_tag="VLS86_08165"
     CDS             643..912
                     /locus_tag="VLS86_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014703984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HSO50509.1"
                     /translation="MTTQYEIPIGEVKVDAELDTSGLLCPLPVYKASIALGQLSKGQV
                     LHLITTDPGSLEDIPALARQLGDTLLAQERLEDRQLFWIEKGDRP"
     gene            909..1418
                     /locus_tag="VLS86_08170"
     CDS             909..1418
                     /locus_tag="VLS86_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HSO50510.1"
                     /translation="MTDTLERLEAVETPAKPKRIALIASKGTLDQAYPPLILATTAAS
                     LGWEAGIFFTFYGLDILHQDRFEKLKVASLANPAGPIQLPNILGALPGATAAATAMMK
                     KWMNTANMPSVPEFIEMTQGLGVRFFACSTTMGVMGVEEGDLIKGAEIAGAAAFLDYA
                     AEADIQLFI"
     gene            complement(1431..>2218)
                     /locus_tag="VLS86_08175"
     CDS             complement(1431..>2218)
                     /locus_tag="VLS86_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSO50511.1"
                     /translation="TWLPVEVEALTRAADMVGAYWEREHAREGLEELAEAKDRFIATV
                     SHELRTPLAAVVGFAGELAEGVESYTSEEITEMVQLIASQSVEMAQLVDDLLTAERAA
                     SGNLTVKPTTVDLLDESRSIVESMRTDVTVTVDGDPTPAWADILRTRQILRNLLTNAA
                     RYGGSQVKISVSSLGDTAVIVVSDDGPGINVVDSERIFDPYYRAQTEEARPDSVGLGL
                     AVARQLARIMGGDLVYRRRAGWTRFELTLPTTSGDAPVLAFPA"
BASE COUNT          389 a          722 c          734 g          373 t
ORIGIN      
        1 gatcctccgc atgctcgggg ccgaggtctg ggagacaccc gacgacctgt gccccgtcga
       61 cctccccaag gacggcgcca tcgcccttgc ccgctcgctg gccgcctccg aacagggcga
      121 gcgctatgtg atgagcgacc agtacgccaa tcccgacaac gtgaccgccc attacgagac
      181 cactggtccc gagatctggc gtcagaccga ggggagaatc ggctggttcg tcaccgggct
      241 cggcaccgga gggacgatca ccggcgtcgg ccgtttcctg aaggagcgcg atccctcgat
      301 acgggtgatc ggtatcgagc cgcagccggg tcatcgcctc cccggtctca agagctttca
      361 ggaggcgaag cagcccgaga tcctcgactg ggacgttgtc gacgacgtga tcagggtgga
      421 cgacgggcct gcctacgaca ccacccggcg gctgtggcgc gaggaggggc tcacggttgg
      481 gccttcgacc ggcgccgtgg tcgatgcaat ccaaaggctc gagaccacca gcgacgacgt
      541 ggtggtgggg atctcggccg attctggctt caaatacacc agctacttcg cggagatcct
      601 cggtgacgag ggtctgcccc gcgtttgacc aaggaggatc gaatgacaac ccaatacgaa
      661 ataccaatcg gagaggtgaa agtcgacgcc gagctcgaca cctccggtct gctctgccca
      721 ctccccgtct acaaggcatc gattgccctg ggccagctat cgaagggtca ggtgctccat
      781 ctgatcacca ccgatccggg gtcgttggag gacatcccgg cgctggcgcg ccagctcggc
      841 gacacgttgc tcgcccagga gcggctcgag gaccgtcagt tgttctggat cgagaaggga
      901 gaccggccat gaccgacacc ctggaacggc tcgaggccgt ggagacgccg gcaaagccga
      961 agcggatcgc cctgatcgcc tccaaaggaa cgctcgatca ggcctacccc ccgctgatcc
     1021 tggccaccac cgcggccagc ctgggatggg aggcggggat cttcttcacc ttctacgggt
     1081 tggacatcct ccatcaggac cgcttcgaga agctgaaggt ggcgtcactc gccaaccccg
     1141 cgggcccgat tcaactgccc aacatcctcg gtgcccttcc cggggccacc gcggcggcca
     1201 ccgccatgat gaagaagtgg atgaacacgg ccaacatgcc gtcggtcccc gagttcatcg
     1261 agatgaccca gggtttgggg gtcaggttct tcgcctgctc aacgaccatg ggggtgatgg
     1321 gagtcgagga gggcgatctg atcaagggtg cggagatcgc cggtgccgcc gcattcctcg
     1381 attacgccgc ggaggccgac atccagctct tcatctaggg cgaggaggct tcaggcggga
     1441 aaggcgagga cgggtgcgtc ccccgaggtg gtggggaggg tgagctcgaa acgggtccaa
     1501 ccggcgcgac gtcggtagac caggtcgccg cccatgatcc tcgccagctg gcgggccacc
     1561 gccaagccga gtcccaccga gtcgggccgg gcctcttcgg tctgcgcccg gtagtagggg
     1621 tcgaagatgc gctcggaatc gacgacgttg atcccgggcc cgtcgtcgct gacgactatc
     1681 accgcggtgt cacccaacga ggacacgctg atcttgacct gactgccccc gtatcgggcg
     1741 gcattggtca ggaggttgcg caggatctga cgggttcgga ggatgtcggc ccatgccggg
     1801 gtcggatctc cgtccacggt cacggtgaca tccgtcctca tcgactcgac gatggatcgc
     1861 gactcgtcga gcaggtcgac cgtcgtcggc ttgacggtga gattccccga agcggcccgc
     1921 tcggcggtga gcaggtcatc gacgagttgt gccatctcga cactctggct ggcgatcagc
     1981 tggaccatct cggtgatctc ttcggacgtg tagctctcga cgccctcggc cagttcgccg
     2041 gcgaacccga ccaccgccgc cagcggggtg cgcagttcgt ggctgacggt ggcgatgaat
     2101 cggtctttgg cctcggcaag ctcttcgagc ccttcgcggg cgtgctcccg ctcccagtag
     2161 gcgccgacca tgtcggcagc gcgggtgagg gcctcgactt ccacgggcag ccaggttc
//
LOCUS       DATITW010000588         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_550060, whole genome shotgun sequence.
ACCESSION   DATITW010000588 DATITW010000000
VERSION     DATITW010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550060"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VLS86_08180"
     CDS             complement(<1..412)
                     /locus_tag="VLS86_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50512.1"
                     /translation="MILLVIPALLLAAVSLASFAGRWLWWLDVLANFRAQYVVGLAIF
                     GVLIAVSRWRRTGLAVLGVALVNLVFVAPLYVGSPGEPVAGAPDLRVLNFNLLSINES
                     YNEVIEYIVAVDPDLVFLHEASRPWEVAIESADLG"
     gene            complement(459..1823)
                     /gene="argH"
                     /locus_tag="VLS86_08185"
     CDS             complement(459..1823)
                     /gene="argH"
                     /locus_tag="VLS86_08185"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HSO50513.1"
                     /translation="MTLWGGRFEAGPGEALWDYTRDESDRRLLEDDVLGSIAHVTMLG
                     SSGILSGEERDGLLDGLAVILDEARSAGFGFVEADEDVHTAVERRLHEIVGDLAGKLH
                     TGRSRNDQVALDVKLYAVRSGWDRIDQLHRYALLLADIAEKHAETVIPSYTHLQQAQP
                     TTLGHHLLAYAWMAKRDIDRFRGALERVDVSPLGAGASAGTSLPIDPEESARLLGMSS
                     VFENSLDAVGSRDHLAEYVFCCAQAMVNLSRFAEEIVLWSTSEFDRLRLDDTVTTGSS
                     ALPQKRNPDIAELVRGRSARVAGDLASIIALQKALPLSYSRDLQEDKRILFDADDVLG
                     SSLAAMTALLNHAEFEAASPVGETVALDLAEALVGRGVPFREAHRAVGKLLLDLEAEG
                     RTLAGATESDLTRAHAGFTTADLGLLDPSSSVARRASRGGGSPESVRSQAADLRAQLA
                     EAVG"
     gene            complement(1820..>2497)
                     /locus_tag="VLS86_08190"
     CDS             complement(1820..>2497)
                     /locus_tag="VLS86_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HSO50514.1"
                     /translation="YSIDANLFHISYEGGILEDPWAPPPADMFRLTADPVDAPDDPEE
                     VVIGFEHGDPVSINERHLDPVPLLEEANRIAGRHGVGRVDIVENRFVGIKSRGVYETP
                     GGTILHLAHRAVESITLDREVLHIRDQLIPKYSEIVYNGFWFSPERSALQAFVDEVQQ
                     PVTGEARLVLYKGGATVNGRRAADGLYDADSATFEADDVYRHADAAGFISLNALRIRG
                     YGGGEVE"
BASE COUNT          460 a          832 c          835 g          370 t
ORIGIN      
        1 acccgaggtc cgcagactca atggccacct cccacgggcg gctcgcctcg tggaggaaca
       61 ccagatcagg gtccaccgcc acgatgtact cgatgacctc gttgtagctc tcgttgatcg
      121 agagcaggtt gaagttgagc acccggaggt cgggggcccc ggccaccggc tcaccgggag
      181 agcccacata gagcggggcg acgaacacga ggttgaccaa ggcgacaccg agcaccgcta
      241 gaccggtgcg gcgccagcgg gacaccgcga tgagcacccc gaagatggcc aggccgacca
      301 cgtactgggc ccggaaattg gccaggacgt cgagccacca cagccagcgc ccggcaaacg
      361 aggcaagact cacggccgcg agaagcaatg ccgggatgac caggaggatc acgcggtcac
      421 gataggaact tcaggtgggc cgggggatgc cggttcgtct aacctacggc ctcggcgagc
      481 tgggcccgaa ggtcggccgc ttgggaccga accgactccg ggctcccacc accccggctg
      541 gcgcgccggg caaccgaaga ggaaggatcg aggagaccga ggtcggctgt ggtgaacccg
      601 gcgtgcgccc gggtcagatc cgactccgtg gccccggcca gggtgcggcc ctcggcctcg
      661 agatcgagca agagcttgcc caccgcgcga tgagcctccc ggaaggggac tccacgtccg
      721 accagggctt cggccaggtc gagcgccacc gtctccccca caggactggc cgcctcgaac
      781 tcggcgtggt tgagcagcgc cgtcatcgcc gccagagaag agccgagaac gtcgtccgcg
      841 tcgaacaaga tccgcttgtc ctcctggagg tcacggctgt aactgagggg gagggccttc
      901 tggagagcga tgatcgaggc gaggtcgccg gcgacccggg ccgaccgacc acgaaccaac
      961 tcggcgatat cggggtttcg cttctgggga agcgcggacg atccggtggt gaccgtgtcg
     1021 tcgagccgca gtcggtcgaa ctccgaggtc gaccagagaa cgatctcctc ggcgaagcgt
     1081 gacaggttga ccatggcttg ggcacagcag aagacgtact cagccaggtg gtccctcgaa
     1141 cccaccgcat cgagggagtt ctcgaacacc gacgacatgc ccaagaggcg ggcactctcc
     1201 tcggggtcga tggggagcga ggttcccgcc gatgcccctg cgccgagcgg ggacacatcc
     1261 acacgttcca gcgccccacg aaagcgatcg atgtcgcgct tcgccatcca ggcataagcc
     1321 agcaggtggt ggccgagggt cgtgggctga gcctgttgga gatgggtgta cgacgggatc
     1381 acggtctcgg cgtgtttctc ggcgatgtcg gccagaagca gcgcgtaacg gtgaagttgg
     1441 tcgatgcggt cccagccgga ccggaccgca tagagcttga cgtccagcgc gacctggtca
     1501 ttccgcgagc gaccggtgtg gagcttgccg gcgaggtcgc cgacgatctc gtgaagccgc
     1561 cgctcgacgg cggtgtgcac gtcctcatcg gcttcgacga agccaaagcc agcggatcgg
     1621 gcctcgtcga gaatcacagc gagcccgtcg aggagaccgt cgcgttcctc gccgctgagg
     1681 atgccgctcg acccgagcat ggtcacatgc gcgatcgagc ccaggacgtc gtcttcgagc
     1741 agccggcgat cggactcgtc cctcgtgtag tcccagaggg cctctccggg accggcctcg
     1801 aaccgccctc cccacagggt cactcgacct cccctccccc gtaacccctg atccgcaggg
     1861 cgttgagcga gatgaaccct gcggcatcgg cgtggcggta gacgtcgtcg gcttcgaaag
     1921 tcgccgaatc ggcgtcgtag agaccgtcgg cggcacgtcg gccgttgacc gtcgccccac
     1981 ccttgtacag caccagccgc gcctccccgg tgaccggctg ctggacctcg tcgacgaaag
     2041 cctgcaacgc cgaccgctcc gggctgaacc agaacccgtt gtacacgatc tcggaatatt
     2101 tcgggatcag ttggtccctg atgtgcagca cctcgcggtc gagcgtgatc gactcgaccg
     2161 cgcgatgcgc cagatgcaag atggtgcccc cgggggtctc gtacacgcca cgagacttga
     2221 tcccgacgaa tcggttctcg acgatgtcga cccggccgac gccgtgccgg cccgcgatcc
     2281 ggttcgcctc ctccaacaac ggcaccgggt cgagatgacg ctcattgatc gaaaccgggt
     2341 cgccgtgctc gaacccgatc accacctctt cgggatcgtc cggggcgtcg accgggtccg
     2401 ccgtcagacg gaacatgtcg gcgggcgggg gggcccacgg atcctcgagg atcccgccct
     2461 cgtaggagat atggaacagg ttggcgtcta tcgagta
//
LOCUS       DATITW010000589         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_309412, whole genome shotgun sequence.
ACCESSION   DATITW010000589 DATITW010000000
VERSION     DATITW010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309412"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /gene="carB"
                     /locus_tag="VLS86_08195"
     CDS             complement(<1..684)
                     /gene="carB"
                     /locus_tag="VLS86_08195"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016351186.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HSO50515.1"
                     /translation="MPRRNDIRSILLIGSGPIVIGQGCEFDYSGTQAAKVLRSEGYRV
                     ILVNSNPATIMTDPEFADATYIEPITAEVVAEIIAREKPDALLPTLGGQTALNVSAEL
                     ARNGVLDQHGGEMIGAGVEAIDRAEDRGRFKETMESVGLEVPRAGYARSMAEAIKIAK
                     EIGYPVMVRPSFILGGGGTGIAQDAEAFIEVARHGLTTSPVGEILVEESLLGWKEFEL
                     EVMRDRNDNA"
     gene            complement(668..1846)
                     /gene="carA"
                     /locus_tag="VLS86_08200"
     CDS             complement(668..1846)
                     /gene="carA"
                     /locus_tag="VLS86_08200"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HSO50516.1"
                     /translation="MTRRPPALLCTADGDTFRGVSVGAPGVTTGEAVFNTSMTGYQEI
                     LTDPSYAGQVVVMTSPHIGNYGVTGLDEQSSSPAVSGLVVRSLSRRDSNWRSEGSVGD
                     YLDRHGVVAIADVDTRRLTRHLRDRGAMPVAIGVDVDKSELQEMAASAPSMLGLDLAS
                     GVTTREPYHLEAVGAPRGRVVAIDLGMKRDILGNLTGRGFEVDVVPSSTDAASILAMA
                     PDGVFVSNGPGDPEPLVAATETIRLLLGRVPLFGICLGHQVLGLAVGASTYKLPFGHH
                     GGNHPVQRLVDGRVEITAQNHGFAVDLWSLTEQEAPLRTGLVDADLLPASVGTRFGPM
                     IPTHQNLNDGTLEGMRLLDIPAFSVQYHPEAAPGPRDAHGHFDEFTRLIQGEAVAAAE
                     "
     gene            complement(1843..>2151)
                     /locus_tag="VLS86_08205"
     CDS             complement(1843..>2151)
                     /locus_tag="VLS86_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSO50517.1"
                     /translation="PRGVIGLETAASVVSVALNGDQTTFFDRMSAAPARIAGLERHGG
                     VVEPGAPANLVLFDPGRSWVPDGFASKSANSPFTGRELVGKVMATIYEGRVVYGGDMS
                     "
BASE COUNT          360 a          743 c          690 g          358 t
ORIGIN      
        1 ggcgttgtcg ttgcggtccc gcatcacctc cagctcgaac tccttccagc cgagcaacga
       61 ctcctcgacg aggatctcgc caaccgggga agtggtcaga ccgtgtcgtg ccacctcgat
      121 gaaggcctcg gcgtcttgcg cgatcccggt gcctccgcct cccaggatga acgagggtcg
      181 aaccatcacc gggtagccga tctccttagc gatcttgatt gcctccgcca tcgacctggc
      241 gtaaccggcc ctaggcacct cgaggccaac cgactccatc gtctccttga accgtccgcg
      301 gtcctcggcc cggtcgatcg cctccacccc ggcgccgatc atctccccac cgtgttggtc
      361 taggacgccg tttcgagcca gctccgcgga gacgttgagc gccgtctgcc cgccgagagt
      421 gggaagcagg gcgtcgggct tctcccgggc gatgatctcg gcgacgacct ccgcggttat
      481 cggctcgatg taggtggcgt cggcgaactc cgggtcggtc atgatcgtgg ccgggttcga
      541 attgaccagg atcacccggt agccctcgga tctgaggacc ttggccgcct gggtgccgga
      601 gtagtcgaac tcacagccct ggccgatcac gatcggcccg gatccgatca agaggatcga
      661 tctgatgtca ttccgccgcg gcaacagcct ctccttggat cagtcgggtg aactcgtcga
      721 agtggccgtg ggcgtcgcgc gggcccgggg cggcctcggg gtggtattgc accgagaacg
      781 ccgggatgtc gagcagacgc atcccctcca gagtcccgtc gttgaggttc tgatgggtgg
      841 ggatcatcgg cccgaaacga gtgccgacgc tcgccgggag gagatcggca tccaccaaac
      901 cggtgcgcag gggtgcttcc tgctcggtca acgaccagag gtcgacggca aacccgtggt
      961 tctgcgccgt gatctcgacc ctgccgtcga cgagccgctg caccggatgg ttgccaccgt
     1021 gatgaccgaa cggcagcttg taggtcgaag ccccgaccgc caagccgagg acctgatgcc
     1081 cgagacagat cccgaacagg ggcacccggc ccaacagcag ccgaatcgtc tccgtcgccg
     1141 ccaccagggg ctcggggtca ccgggcccgt tggagacgaa gaccccgtcg ggcgccatgg
     1201 cgaggatgct cgccgcatcc gtggacgagg gcaccacgtc gacctcgaag ccgcgcccgg
     1261 tcaggttgcc gaggatgtca cgtttcatgc ccaggtcgat ggccaccacc cgacccctgg
     1321 gcgccccgac ggcctcgagg tgatagggct ctctcgtggt caccccggag gccagatcga
     1381 gaccgagcat ggaaggcgct gaggccgcca tctcctgcag ctcggatttg tcgacgtcga
     1441 cgccgatggc caccggcatc gcgccccgat ctcgaagatg gcgggtcagc ctcctggtgt
     1501 cgacatctgc gatcgccacc accccatgac gatcgaggta gtccccgacc gacccttccg
     1561 agcgccaatt ggagtcccga cgcgacaggc tgcggaccac cagaccagag actgcggggc
     1621 tcgacgactg ctcatcgagg ccggtgactc cgtagttgcc gatgtgcggg ctggtcatca
     1681 cgaccacctg gccggcatag gagggatcgg tgaggatctc ctgatacccc gtcatcgagg
     1741 tgttgaagac cgcctcgccg gtggtcaccc cgggtgcgcc gaccgagacg ccccggaatg
     1801 tgtcgccgtc ggcggtgcag agcagagccg gtgggcgtct ggtcatgaca tgtcgcctcc
     1861 gtacacaacc cggccttcgt agatcgtcgc catcaccttt cccaccaatt ccctcccggt
     1921 gaacgggctg ttggcggatt tggaggcaaa cccgtcgggc acccagcttc gccccgggtc
     1981 gaagagcacg aggttggccg gcgcccccgg ctcgacgaca cccccatgcc gctccaatcc
     2041 cgcgatccgg gccggtgcgg cgctcatccg gtcgaagaac gtcgtctgat cgccgttgag
     2101 agccacagaa acgaccgagg cggcggtttc gagcccgatg acccctctcg g
//
LOCUS       DATITW010000590         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_590047, whole genome shotgun sequence.
ACCESSION   DATITW010000590 DATITW010000000
VERSION     DATITW010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590047"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VLS86_08210"
     CDS             complement(<1..281)
                     /locus_tag="VLS86_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50518.1"
                     /translation="MSGETRYWTPGDFEQVNGFPRYPVSEMDRRHRWATDTMGERGID
                     LLIVGGPTGPLETSVQYFSNWPSQVQSYVLFASAEPPILLVRLWNHVPDA"
     gene            complement(383..1756)
                     /locus_tag="VLS86_08215"
     CDS             complement(383..1756)
                     /locus_tag="VLS86_08215"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSO50519.1"
                     /translation="MASDRKAIWGDEPKPLMPYSPAIKAGGWVFFAGQLASDFKGGVA
                     PEAGGDGRNPNVDNQLELQSRFVMQNLKKTADAAGIDLGTDIVRIYQWFTSPYPTIEE
                     FAEGNTWPRISITPYLDARNEFIFEPRPASTGMGIRETGLLVKDTILEVDMIGIDPAA
                     VPGPSKGTAVPEGTPSPLAGYSPAIERGDWVFLAGEIPVDWVGDWGRSEYYGTPSGVA
                     LKARTNPNFWYDSDIERQTDFTLEKQAAIAEAAGTSLDRAVKATVYLGHPSEFEGMDR
                     VWKRWFPENPPARVVIPYMGLGGRGSRVEIAFKLLSGTSDLSIETIEAPGVPPGLGHE
                     PQAVKAGKFLFFSTVSPFDHKGSLDESTKRHPSFPFYGQPPKLEMRYMLDKAAKICEA
                     AGTSLDQICRRQAFHDDFTHFAQSIEEWAAHFDGDKPASTTLEVGGPLLVPGSHVLLD
                     LIGYCPD"
     gene            complement(1803..2606)
                     /locus_tag="VLS86_08220"
     CDS             complement(1803..2606)
                     /locus_tag="VLS86_08220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HSO50520.1"
                     /translation="MKTDMYCYMENRKRLTLPPGVPGYADAFETWLDPAHTAVLEIDM
                     HRGHVGEEDRLTLAVPRARERVPAHNRFQAECRRVGIPIIHVQHWQRDGGIDDLNNRK
                     HNRMANWRVLYYLYVEDNELMDEHSWEGTKWLDLITEEEEGDYYVRTKKRLSGFYPTD
                     LEFLLHNLDVNNVVLTGTMTDACVLSTAFDAANRDFRVIVPRDVVGGYSEEAEHGALQ
                     VISLHLGLVVDAPALLAEYFARVGMELPSDLVGVDDINEVPGLLQRTTV"
     gene            complement(2646..>3048)
                     /locus_tag="VLS86_08225"
     CDS             complement(2646..>3048)
                     /locus_tag="VLS86_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50521.1"
                     /translation="VFGIIEAAAEADGQVWVVPSYYESQSLDPDHVMTSAIHGLTFAS
                     SSIIEQGAGGGFEESDFLDFDALNNPEIDAPLYPNIEGMLSADELAQYEDIVAKVRSG
                     EITIPDETTGDPTLGAEGTGAAIDPASIGCG"
BASE COUNT          539 a          974 c          950 g          585 t
ORIGIN      
        1 gcatccggaa cgtggttcca gagtcggacc aagaggatcg ggggctcggc gctcgcgaag
       61 aggacgtagc tctgcacctg gcttggccag ttactgaagt attggaccga cgtctcgagc
      121 gggccagtgg gaccacccac gatgagcagg tcgatgccac gctcgcccat cgtgtctgtg
      181 gcccaccgat gccggcggtc catctcgctt accgggtagc gggggaaccc gttgacctgc
      241 tcgaagtcac ctggtgtcca ataccgggtt tcaccagaca cgatcagccc ctcgcgacct
      301 ggttggctcc accatgaccg cgctcgtatg gcgcgcgtct gcaaggcggg agcagtcgga
      361 gggaccggac ccttcttttc gatcagtcgg ggcagtagcc gatgagatcc aggagcacat
      421 gggagcccgg caccaacagc ggcccaccga cctcgagggt ggtggacgcc ggcttgtcgc
      481 cgtcaaaatg ggccgcccac tcctcgatcg actgcgcgaa gtgggtgaag tcgtcgtgga
      541 acgcctgccg gcgacagatc tggtcgagcg aggtgcccgc tgcctcacag atcttcgccg
      601 ccttgtcgag catgtaccgc atctcgagct tgggtggctg cccgtagaac gggaagctgg
      661 gatgccgttt tgtcgattca tccagagacc ccttgtggtc gaatggcgac acggtcgaga
      721 agaacaggaa ctttccggcc ttgacggcct gcggctcgtg accgagaccg ggaggcaccc
      781 ccggggcctc aatggtctcg atcgagagat cggacgtgcc cgagagcagt ttgaaggcga
      841 tctcgacgcg cgagcccctg ccaccgagac ccatgtaggg gatcacaacc cgggccggtg
      901 ggttctcggg gaaccaccgt ttccacactc tgtccatgcc ttcgaactcg gacgggtgcc
      961 cgaggtagac cgtggccttg accgctcggt ccagagaggt tccagcagcc tcggctattg
     1021 ctgcctgctt ctccagagtg aagtcggtct ggcgctcgat gtcggagtcg taccagaagt
     1081 tggggttggt cctggccttg agggccacac cgctcggggt cccgtaatac tcggaacgcc
     1141 cccagtcacc gacccaatcg acggggatct ctccggcgag gaagacccag tcaccccgct
     1201 cgatggctgg ggagtagccg gcgagcgggg agggcgttcc ctcgggcacg gctgtccctt
     1261 tgctcggccc agggacagca gcagggtcga tcccgatcat gtccacttcg aggatggtgt
     1321 cctttaccag cagaccggtc tcgcgaatcc ccattccggt ggaggccggc cgtggctcga
     1381 agatgaactc gttccgggcg tcgaggtacg gggtgatcga gatgcgcggc caggtgttcc
     1441 cctcggcgaa ctcctcgatc gttgggtagg gcgaggtgaa ccactgatat atgcggacga
     1501 tgtccgtacc aaggtcgatg cccgcggcat cggcggtctt cttcaggttc tgcatcacga
     1561 accgtgactg caactcgagc tggttgtcca cgttggggtt gcgcccatcg ccaccggctt
     1621 cgggtgccac cccgcccttg aagtccgacg ccagttggcc ggcaaagaac acccacccac
     1681 ccgcctttat tgccgggctg taaggcatca acggcttggg ctcgtctccc cagatcgcct
     1741 tgcggtcact tgccatggtc tctcctctcg cgggaatgtg tctggaccac tcagctcgcc
     1801 gtctaaaccg ttgtccgttg aagcagaccg ggcacctcgt tgatgtcgtc gacgccgacc
     1861 agatctgagg gcaattccat gccgacccgg gcgaagtatt cggccaacag ggcaggggcg
     1921 tctaccacca ggccgaggtg cagcgagatc acctgaagcg cgccgtgttc cgcctcctcc
     1981 gagtaacccc cgaccacgtc cctcggcacg atcacccgaa agtcgcggtt ggcggcgtcg
     2041 aaggcggtgg acaagacaca ggcgtcggtc atcgtcccgg taagcaccac gttgttgaca
     2101 tccaggttgt gcaacaggaa ttccaaatcg gtcggataga accccgacaa cctcttcttg
     2161 gtccggacgt agtagtcgcc ctcctcctct tcggtgatca ggtcgagcca tttggtcccc
     2221 tcccaggagt gctcgtccat caactcgttg tcttcgacgt acaggtagta gagcacccgc
     2281 cagttggcca tccggttgtg cttgcggttg ttgagatcgt cgatccctcc gtcccgctgc
     2341 cagtgctgga cgtggatgat cggaatcccc acccttcgac actcggcctg gaagcggttg
     2401 tgggcgggga cgcgctctcg ggcacggggc accgccagcg tcagccggtc ctcctcaccc
     2461 acatgaccgc ggtgcatatc gatctcgagg actgcggtgt gggcagggtc tagccaggtc
     2521 tcgaatgcat ccgcgtagcc ggggacaccc ggcgggaggg tcaacctctt ccgattctcc
     2581 atgtagcaat acatgtccgt cttcatgctt ccacctcctc gtcgggtgtc ctcacagtca
     2641 tggcatcaac cgcagccgat ggacgccggg tcaattgcag cgccggtccc ttcggcaccg
     2701 agggtgggat cgccagtggt ctcgtccggg atggtgatct caccggagcg caccttggca
     2761 actatgtcct catactgggc caactcgtcc gcgctgagca tcccctcgat gttggggtag
     2821 agcggagcgt cgatctcagg attgttgagg gcgtcgaagt ccaagaaatc gctctcctcg
     2881 aatccaccac ccgccccctg ctcgatgatc gacgaggaag cgaaggtaag accgtgaatc
     2941 gccgaggtca tcacatggtc ggggtcgagc gactgactct cgtaatacga gggcaccacc
     3001 cacacctggc cgtcggcctc ggcggcagct tcgattatcc cgaacacc
//
LOCUS       DATITW010000591         2957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_630167, whole genome shotgun sequence.
ACCESSION   DATITW010000591 DATITW010000000
VERSION     DATITW010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630167"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VLS86_08230"
     CDS             <1..484
                     /locus_tag="VLS86_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO50522.1"
                     /translation="STPTSTGVIVSGEGAGRWAVAMVQGLYIVAATLLLFRVNWGDPL
                     GAALILVTFAAVGAGAGVLMGAVFKNDQQAGGIGVVLSLGLAALGGCMFPIELFSPTM
                     QQVAHLTPHAWALDGFAELVRREGTVVDILPELGVLTAYAVVLLLLASWRLRVVLTRA
                     "
     gene            502..1119
                     /locus_tag="VLS86_08235"
     CDS             502..1119
                     /locus_tag="VLS86_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50523.1"
                     /translation="MADQNVNAVGCLGRVLTFFGVVWIAIIVLGGMGLLSDAGFSGEF
                     LAGFGGSIIPGLLLLGAGRALRRRGAAMDDQSASTPTPDQLHSEGSRVPSIRLEPTPA
                     PPRSPTPTTIPMPPTESTPPSRSLPRPSAPKPPAPKPGPVDPMVKTRGDVAKSIESAL
                     SGLEEASDEPAREESAPFGVEPGRHKTSQELVDEARRRWGVDRPR"
     gene            complement(1116..>2957)
                     /locus_tag="VLS86_08240"
     CDS             complement(1116..>2957)
                     /locus_tag="VLS86_08240"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HSO50524.1"
                     /translation="VSGQVDPITSYVTRRAWLVLDQAERRLRGNRFKVPRFVPEAILS
                     RREFLDSVDAVAAESGMTPALAREKAERYLHEIAATHSPYVIDLIANAIHKLYSQGYG
                     DIKYSDDEVKRLTALGAGHTMVFLPSHRSNLDRLSLQFMLWENDLPPNHTAGGINLNF
                     FPIGPLLRRTGVFFIRRTFKDNLLYKTVLRSYLDYLVEKRFPLEWYMEGGRSRSGKLM
                     PPRLGLLSYVVDAIRRGKCDDIQLIPVSIAYDQIQDVPEYAREAQGKEKERETIGWLF
                     RAVRSLRRRYGDIHIRFGEPVSVRDMLETIEGDDETSIGLQKLGFEVMYRIGQVTPIT
                     PTAFVTVVLLAVRGSARSADEIAEAASRLLEFARARDIPTTEDFDPTEPGRVAAVLDW
                     LAENDNVSSHEALGRKVFRLDDEQMIRISYYRNVIVHFFVNRALAEMAIGSVAESGAE
                     HPDRVREAMLAYRDLLKFEFFFPEKDQFLSDVEREIAIDVPGWDTVLSSIGAVEVLAK
                     LGSPVAYWAVLPFLDAYQVVGDELESLKKDFDEKSFLKACLDRARMYRIEERLISGES
                     ASQVLFKSALALARNRDLIDRSDDVHARRAAFAAEVREARQMAAAGL"
BASE COUNT          501 a          975 c          962 g          519 t
ORIGIN      
        1 ctcgactcca acctcgaccg gggtcatcgt cagtggcgag ggtgcgggtc ggtgggccgt
       61 ggccatggtg cagggcttgt acatcgtggc ggcgacgctt ctcctgttcc gggtcaactg
      121 gggggacccg ctgggcgccg ccctgatcct ggtcaccttc gccgcggtcg gcgccggagc
      181 cggtgtgttg atgggtgcag tcttcaagaa cgaccagcag gccggtggca tcggcgtggt
      241 gctgagcctc ggcctggctg cgctcggggg gtgcatgttc ccgatcgagc tcttctcacc
      301 gaccatgcaa caggtggccc atctgacacc acatgcctgg gccctcgacg ggttcgccga
      361 actggtgcgt cgcgagggca cggtggtcga catcctgccc gagctcggcg tgctgacggc
      421 ctacgcggtc gttctcttgc tgctggcttc atggcgtctc agggtcgtcc tcacccgagc
      481 ttgatgtttg aatgattgtc gatggctgac caaaacgtca acgctgtggg ctgcctgggc
      541 cgggtgttga ccttcttcgg cgtggtgtgg atcgccatca tcgtcctcgg cggaatgggg
      601 ctcctctccg atgcaggatt cagcggcgag ttcctcgccg gcttcggtgg gagcatcatc
      661 cccggcctcc tcctgctcgg ggcgggtcgg gccctccgac gacggggtgc ggccatggac
      721 gatcaatcgg cgtcgacccc cacgccggac caactccact ccgaggggag ccgcgtcccg
      781 tcgatccgac tggagccgac cccggcgccg ccccgttcgc cgacgccaac cacgatccca
      841 atgccaccga ccgagagcac cccacccagt cgttccctgc ccaggccctc cgcacccaag
      901 ccccccgcac ccaagcccgg accggtggac ccgatggtca agacccgggg cgacgtcgcc
      961 aagagcatcg aatcggccct ttccgggctc gaagaggcct ccgacgagcc ggcccgcgag
     1021 gaaagcgccc cattcggcgt cgaaccgggg cgacacaaga cttcccagga gctggtggac
     1081 gaggctcgcc ggcgatgggg tgtcgaccgg ccccgctaga gaccggccgc ggccatttgc
     1141 cgggcctcgc gcacttccgc ggcgaaggcc gccctgcggg catggacgtc gtcggaccga
     1201 tcgatgagat cgcgattccg ggccagggcc aaggcggact tgaacagcac ctggctggca
     1261 ctctcgcctg agatgagccg ctcctcgatt cggtacatcc gggcgcgatc gagacatgcc
     1321 ttgaggaacg acttctcgtc gaagtccttc ttcaggctct ccagctcgtc gcccacgacc
     1381 tggtaggcgt cgaggaacgg gagcacggcc cagtaggcga cgggtgaccc cagtttggcc
     1441 agcacctcaa ccgccccaat cgaggagagc acggtgtccc aacctgggac gtcgatggcg
     1501 atttcgcgct cgacatcgga gaggaactgg tccttctccg ggaagaagaa ctcgaacttg
     1561 agcaggtcac ggtaggcaag catcgcctcc cgaacccggt ccgggtgctc cgcccccgat
     1621 tccgcaaccg acccgatggc catctcggcc agggcacggt tgacgaagaa gtggacgatc
     1681 acgttccggt agtacgagat ccggatcatc tgctcgtcat cgagccggaa gacctttctc
     1741 cccagggcct cgtggctgct cacgttgtcg ttctcggcga gccagtcgag caccgcggcc
     1801 acccgacctg gctccgtcgg gtcgaagtcc tcggtggtcg ggatgtcacg cgccctggcg
     1861 aactcgagga gccgagaagc agcctcggct atctcgtcgg ccgaccgggc cgagccgcga
     1921 accgcgagaa ggacaacagt gacgaacgcg gtcggcgtga tcggcgtcac ctgaccgatt
     1981 cgatacatga cttcgaagcc gagcttctgg agcccgatcg aagtctcgtc gtcgccttcg
     2041 atcgtctcca gcatgtctct caccgatacc ggctcgccga accggatgtg gatgtcgccg
     2101 tagcggcgtc ggagcgagcg caccgcccgg aacagccacc cgatcgtctc ccgctccttc
     2161 tccttgccct gcgcctctcg ggcgtactcg ggtacatcct ggatctggtc gtaggcgatc
     2221 gaaactggta tgagctgtat gtcgtcgcat ttcccccttc gtatggcgtc gacgacgtaa
     2281 ctgagcagcc cgagacgggg aggcatcagc ttccccgagc gggagcgacc gccctccatg
     2341 taccactcca gcgggaacct cttctcgacc aggtagtcga gataggagcg caggacggtc
     2401 ttgtacagca ggttgtcctt gaaggtgcga cggatgaaga agaccccggt gcggcgaagc
     2461 aaggggccga tcgggaagaa gttgaggttg atccctccag ccgtgtggtt gggcgggaga
     2521 tcgttctccc agagcatgaa ctggagactc agtcgatcca gattggaacg atgcgatggg
     2581 agaaagacca tcgtgtgccc cgccccgagc gcggtgagac gcttgacctc gtcgtcggag
     2641 tacttgatgt ccccgtaacc ttggctgtag agcttgtgga tggcgttggc gatgagatcg
     2701 atgacatagg gggagtgggt agcagcgatc tcgtgcagat agcgctccgc cttctcccgg
     2761 gccagggcgg gggtcatccc cgactcggcc gccaccgcgt caaccgagtc gaggaactca
     2821 cgacgagaca ggatcgcctc gggcacgaat ctcggcacct tgaagcgatt gccgcggagc
     2881 ctgcgctcgg cctggtcgag aaccagccag gccctcctcg tcacgtagct cgtgatcgga
     2941 tccacctgac cggagac
//
LOCUS       DATITW010000592         1550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_630225, whole genome shotgun sequence.
ACCESSION   DATITW010000592 DATITW010000000
VERSION     DATITW010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630225"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VLS86_08245"
     CDS             complement(<1..1242)
                     /locus_tag="VLS86_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSO50525.1"
                     /translation="MERTTGYARLGPDRIAFEVVTSTPEPSIDLVLTAGSFGAIDADW
                     EDPQAELFFRRLASFTRVIRFDRRGTGGSDPLTLDALPPWESFVEELECVMEEVGSDR
                     AAIMTAYDAGPMGMLFAATKPERTAALILVNTSSKYVASDDYPIGVAAEAAEDLNRTM
                     AERWGSDQHVLLQVPSRADDARFRTWYGKKTRSIAGPAAAAAYFRAMFTADARSLLPA
                     IHVPTLVLHRTSYRFMPIEHGRFLAEHIQGARMVELPGSDGPLYWDHPDEALVAIEEF
                     LTGVTPATPTDRVLATVLYTDIVDSTRLLAEMGDARWRGVLDTHDDLAGRLAFENGGR
                     LIKTTGDGVLATFDGPGRGIRFATGFRDQLHTIALTIRSGIHTGEVEMRGGDVGGMAV
                     HLAARIMGEAHDGEILVSRTVK"
     gene            complement(1320..>1550)
                     /locus_tag="VLS86_08250"
     CDS             complement(1320..>1550)
                     /locus_tag="VLS86_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA containing CoxE family protein"
                     /protein_id="HSO50526.1"
                     /translation="VIFTGDARNNHRMDDAGLAILRRIHDRSKQVYWLNPESRGDWGD
                     EDSLMPEFKRFCTGVYEVRNLNQLQEFVLEIA"
BASE COUNT          272 a          484 c          516 g          278 t
ORIGIN      
        1 tttcacggtt cgggagacaa ggatctcccc gtcgtgggcc tcgcccatga ttcgggcggc
       61 gagatggacg gccatcccgc cgacgtctcc accccgcatt tcgacctctc cggtgtggat
      121 gccggagcgg atggtcaggg cgatcgtgtg gagttggtcg cggaaaccgg tcgcaaaccg
      181 aattccccgc ccgggcccat cgaaggtcgc gagcacgccg tcgccggtgg tcttgatcag
      241 ccggcctccg ttctcgaatg cgagccgtcc cgcaaggtcg tcatgtgtgt cgaggacacc
      301 gcgccaacgc gcgtcgccca tctccgccag gagccgggtc gaatcgacga tgtcggtgta
      361 gaggacggtg gccagaaccc tgtccgtcgg ggtggcaggg gtgaccccgg tcagaaactc
      421 ttcgatcgcg acaagcgctt cgtcgggatg atcccaatag agaggaccat ccgagccggg
      481 gagttccacc atgcgggcgc cttggatgtg ctccgccaag aaccgcccat gctcgatcgg
      541 catgaagcga taggaggtcc gatgcaagac gagcgtgggc acatggatag ccgggagcaa
      601 ggagcgggcg tcggcagtga acatggcccg aaagtaggcg gcagccgccg cggggcctgc
      661 aatcgagcgc gttttcttcc cgtaccaggt tcggaaccgc gcgtcgtcgg cccggctcgg
      721 cacctggagc aacacgtgct ggtcgctgcc ccagcgttcg gccatcgtcc gattcaggtc
      781 ttctgcggcc tcggcggcaa ccccgatcgg gtagtcgtca gaggccacgt acttgctgct
      841 tgtgttgacc aggatcagcg cagcggtgcg ttccggcttg gtggccgcga agagcatgcc
      901 catgggcccc gcgtcgtagg cggtcatgat tgccgctcga tcgctgccca cctcctccat
      961 cacgcattcg agctcttcga cgaaggactc ccatggaggc agggcatcta gggtgagggg
     1021 gtcggagcct cccgttccgc gacggtcgaa ccggatgacc cgagtgaacg aagcgagccg
     1081 gcggaagaag agctctgcct gtggatcctc ccagtccgca tcaatggcac cgaagctacc
     1141 ggcggtcagc accaggtcga tcgatggttc cggggtggag gtgacgactt cgaaggcgat
     1201 ccgatccggg ccgagtcgtg cgtagccggt cgtgcgctcc acgagccgag gatagagctc
     1261 tgaccgccga ggcgccttgg cgcgaaggac cgttcgtcga ggtctctctt cgagccctct
     1321 caggcgatct cgaggacgaa ctcttggagc tgattgaggt tgcgcacctc atagaccccg
     1381 gtgcaaaacc gtttgaactc gggcatcagc gagtcctcgt ctccccaatc accccgggat
     1441 tcggggttga gccaatagac ctgcttggag cgatcgtgga tccggcgcaa tatcgccaat
     1501 cctgcgtcat ccatacggtg gttgttgcgg gcgtcaccgg tgaagatcac
//
LOCUS       DATITW010000593         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_109625, whole genome shotgun sequence.
ACCESSION   DATITW010000593 DATITW010000000
VERSION     DATITW010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109625"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..554)
                     /locus_tag="VLS86_08255"
     CDS             complement(15..554)
                     /locus_tag="VLS86_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50527.1"
                     /translation="MWDSKRRRRLVGAMTTALVLIISLSLVLYGTPEASATGGQDHRQ
                     EIQCDEDHGTTTTKPKTTTTTARCKPTTTTTEVVTTTAEVTTTAEVTTTEEVTTTMRP
                     TTTVPNEVLPTVVTTTAEETTTTGKATTTTAAVVSSVAGEAATLPFTGSSDMGWVVLA
                     ASAITMGSILVAGSRRQDT"
     gene            complement(809..1582)
                     /locus_tag="VLS86_08260"
     CDS             complement(809..1582)
                     /locus_tag="VLS86_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSO50528.1"
                     /translation="MSTALVSPHHLRASSKDLLALARYWEWERTVIHELPRVVAEDDI
                     EILFQPIYRLVRGVPVARGFEALSRFPTAPRIPLGLWFKTAHDLEMSKELELAALRAS
                     IKSASKLSDQAFLCVNASLESVSDLMELIPVDLDQRLIIDLPYSSVRDPGAEDLFHVI
                     REGGAGIAIDDVPLDDLHLLRPTLLELRPDCIKVDVLVGLAESPMARFNLAEGAAWCQ
                     QAGINLIAERVERMSDLSVLGGVGVEWAQGYSLSRPVDL"
     gene            1862..1954
                     /locus_tag="VLS86_08265"
     tRNA            1862..1954
                     /locus_tag="VLS86_08265"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1896..1898,aa:Ser,seq:gct)
     gene            complement(1933..>2268)
                     /locus_tag="VLS86_08270"
     CDS             complement(1933..>2268)
                     /locus_tag="VLS86_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014983325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HSO50529.1"
                     /translation="TGIHALGWSRQQAIDWLLKWTPIGRLTIEQEVDRYIGMPGQALS
                     YKMGQLEIMRLRAEAEQRLGASFDIKGFHDTLLTSGAITLPLLAELAGSWVEQVGSGG
                     AESGGEGGI"
BASE COUNT          391 a          727 c          730 g          420 t
ORIGIN      
        1 gtcagcgcaa gacgctaggt gtcctgtctc ctcgagcctg cgacgaggat cgagcccatc
       61 gtgatcgcgc ttgctgccag aaccacccat cccatgtcac tggaaccggt gaagggaagc
      121 gtcgccgcct ctcccgctac cgagctgacc accgccgcag tcgtggtggt cgctttcccc
      181 gtggtggtgg tctcttccgc tgtggtagtc accaccgtcg gcagcacctc gttcgggacc
      241 gtggtcgtcg ggcgcatggt ggtcgtgact tcctcggtgg tggtgacctc cgcggtggtg
      301 gtgacctccg cggtagtcgt gaccacctcg gttgtggtcg tggttggttt gcacctggca
      361 gtggtggtgg ttgtcttcgg cttggtggtg gtcgtgccgt ggtcctcgtc gcactggatc
      421 tcttggcgat gatcctgccc gccggtggcc gacgcctcag gcgtgccgta caggacgagg
      481 gacaaggaaa tgatcagaac cagggccgtt gtcattgctc ccaccagcct gcgtcgccgc
      541 ttgctgtccc acatctcttc gaccctccca atcatggaga ggccggtctc actattggct
      601 ccacccgacc cgagtgaccg ctgctattgg tcgggtctca atgcctcctc tttcccctcg
      661 cgatgacctc gaagacggtg gccttgcggt cacgcctcgc ggccctcgcg ctcatgatcc
      721 tgccctcgat cgagcacgca tagagccatt gctctcgaat tggccgccac aagtcctcaa
      781 cggggctgcg gcgctgaggt cgtccgttct agaggtcgac gggtcgagac aggctgtaac
      841 cctgggccca ctccacgccg acgcctccga gcacggagag atccgacatc cgctccacgc
      901 gctcggcgat caggttgatc ccggcctgct gacaccaggc tgcgccttcg gccagattga
      961 accgcgccat ggggctctcg gcgagcccga ccaggacgtc gaccttgatg cagtccggtc
     1021 gcagctcgag cagggtgggg cggaggaggt gaaggtcgtc gagcgggaca tcgtcgatcg
     1081 cgatcccggc accgccctca cggatgacgt ggaacagatc ctcggccccg ggatctcgga
     1141 ccgacgagta gggaaggtcg atgatgagcc gctgatcgag gtccaccgga atcagctcca
     1201 tcaggtcgct cacgctctcc aacgacgcgt tgacgcagag aaaggcctga tctgacagct
     1261 tcgacgcaga cttgatcgag gcccggaggg cagccaattc caactctttg ctcatctcga
     1321 gatcatgggc ggtcttgaac cacagaccca agggaatgcg cggtgctgtc gggaacctgg
     1381 agagggcctc gaagccacgg gcgacgggga caccgcgaac cagtcggtag atgggctgaa
     1441 agaggatctc gatgtcgtcc tcggccacta cccggggaag ctcatggatc accgtccgct
     1501 cccactccca gtatctggcg agggcgagga ggtctttgga cgaggctctg aggtggtggg
     1561 gggaaaccag agccgtgctc actcccgggt aatcgacagc ggccgaccac aacttgagcg
     1621 acggggcgcg agtgatcagt gcggcgtccc atccgctgac acaagcttgg tatgagcggc
     1681 gtggtgtggc cagaggccgg gagacccatc aaggctcgag cgttctgggg aggctcaggt
     1741 ccggatgtga cccctgtccg tcccaattaa gggggcatca ggcgacctgg cgggagactc
     1801 ccgaaacgtc ccagcccctc accacgtata acgtatggaa cggtgccgct tcggcaccca
     1861 cggagaggtg gccgagtggt cgaaggcgct cgcctgctaa gcgagtaggg ggttttaagc
     1921 ctcctcgagg gttcaaatcc ctccctctcc gccagactca gcgccgccgg agccgacctg
     1981 ctccacccag gacccggcca gctcggcaag caggggcagg gttatcgccc cgctggtcag
     2041 caacgtgtcg tgaaagccct ttatgtcaaa cgaggctccg agacgctgct cggcctcggc
     2101 ccgaagacgc atgatctcaa gctggcccat cttgtaggag agggcctggc cgggcatccc
     2161 gatgtaacgg tccacttcct gctcgatggt cagccgtccg atcggcgtcc acttcaggag
     2221 ccagtcgatc gcctgttgcc gggaccaacc gagcgcatgg atcccggt
//
LOCUS       DATITW010000594         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_789827, whole genome shotgun sequence.
ACCESSION   DATITW010000594 DATITW010000000
VERSION     DATITW010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789827"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VLS86_08275"
     CDS             <1..292
                     /locus_tag="VLS86_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007029271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50530.1"
                     /translation="VGPVVAQQAGFDAGCNVEFISVAGDVLTMRVWERGIGETLACGT
                     GMAAAAAVALNGADGLVTVDVPGGTGSVEIRDGIAWLTGPAEYSFRGVWDQG"
     gene            complement(283..903)
                     /gene="lexA"
                     /locus_tag="VLS86_08280"
     CDS             complement(283..903)
                     /gene="lexA"
                     /locus_tag="VLS86_08280"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HSO50531.1"
                     /translation="MLSERQQQVLEYIRQTVTDRGYPPSVREIGDAVGLSSPSTVHSH
                     LSALVEAGALKRDPTKPRAIVVVDDQASSVSRASSRMRDVPVLGRIAAGTPILAAEHV
                     EEVLPLPVDLVGNDPVFLLEVKGDSMIGAGIFPGDLVAIRSQKDARDGEIVAALIDGE
                     DATVKRLRRKDGKVILESENPNYEPMVFTEGVELIGKVVSVLRRYP"
     gene            1156..1374
                     /locus_tag="VLS86_08285"
     CDS             1156..1374
                     /locus_tag="VLS86_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HSO50532.1"
                     /translation="MVFLMIGGSADAEGPTPVVEYVVAPGDTLWQIAAVRTEAGGDVR
                     RLVSDIVELSGVENGMIIPGQILLLPGG"
     gene            1402..1851
                     /gene="nrdR"
                     /locus_tag="VLS86_08290"
     CDS             1402..1851
                     /gene="nrdR"
                     /locus_tag="VLS86_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012360135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HSO50533.1"
                     /translation="MLCPTCGAPDTRVIDSRPAEGGQSIRRRRVCETCEARFTTYERL
                     EPQLMVRKRNGRLEAFSSAKLAAGISAAFADRPVSGSAIQSIAEEVEHSLRLQGSQVS
                     SEDIGHQVLERLRALDEVAYLRFASVYKDFQDAADFEKEVAALESSE"
     gene            complement(1840..>2386)
                     /locus_tag="VLS86_08295"
     CDS             complement(1840..>2386)
                     /locus_tag="VLS86_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50534.1"
                     /translation="NLTATDLFDVDNAARGLFDASGTLPGSETAIRSIATDVASLSFE
                     VSRQLRDGLAYRGALEPILLAPALETDPAFTDLATAALDFTEWRTHFDSVRSALPKGV
                     ADEVTLSLEEFSSSFETRQGVYLDAIRSNDQRAAETAMQTLESDLGAIRELMMTRMTN
                     LAGDVDSEVDAARDLIGRLLR"
BASE COUNT          420 a          729 c          777 g          460 t
ORIGIN      
        1 cgtgggcccg gtagtggcac aacaggctgg attcgatgcc ggttgcaacg ttgagttcat
       61 ctcggttgcc ggtgacgtcc tcaccatgcg ggtctgggag cgtgggatag gggagacgct
      121 ggcctgtggc actggcatgg cggcggccgc agcggtggcc ctcaatggcg ccgacggctt
      181 ggttacggtc gacgtgccgg ggggaaccgg gtcggtggag atccgcgatg ggatcgcctg
      241 gctgaccgga ccggcggagt attccttccg cggggtctgg gatcagggat agcgtcggag
      301 gacgctgacg accttcccga tcaactcgac gccctcggtg aacaccatcg gctcatagtt
      361 cggattctcc gattcgagga tcaccttgcc gtctttgcgg cggaggcgtt tcactgttgc
      421 atcctcgccg tcgatcaagg cagccacgat ctcgccgtct cgggcgtcct tctggctgcg
      481 gattgccacc aagtcgccgg ggaagatccc ggcgccgatc atggagtcac ccttgacctc
      541 gagcaagaac accgggtcgt tgcccacgag gtccaccggc agcggcagaa cctcctcgac
      601 atgctccgcg gccaggatcg gggtgccggc tgcgatccgg cccaacaccg gcacatcacg
      661 catccggctc gaagcccggc tcaccgagga ggcctggtca tcaaccacga cgatggcgcg
      721 gggcttggtg gggtctcgct tgagggcgcc ggcctcgacc aacgccgaga ggtgggagtg
      781 gaccgtggat ggcgacgaaa gacccacggc atcgccgatc tcgcggaccg atggcgggta
      841 accccggtcg gtcacggttt gccgtatgta ctccagaacc tgttgctgtc gctcgctgag
      901 cactgactcc tcctcgaact gatgttctat ggaggctaac cgaacacggg ttcgaaacca
      961 aacacctgtt cgattctccg atctacgccg tttcgacttg acagcgaaca tctgttcgat
     1021 agagtggcga acgcatgttc gatacgaact gcatccagca aagagcccat caagaccgat
     1081 caggagggag accaaatgca gaaagcaaca cttcagctgt ccgtccggct gtgcttgctt
     1141 ctagccgcaa tctgcatggt cttcctcatg atcgggggct ctgccgacgc cgaagggccc
     1201 accccggtcg tcgagtatgt ggtcgcaccc ggtgacacgc tatggcagat tgctgcggtc
     1261 aggaccgagg ccggtggcga cgttcggcgt ctggtctcgg acatcgtcga gttgagcggg
     1321 gtcgagaacg ggatgatcat ccccggtcag atcctgcttc ttcccggggg ctgagcgatc
     1381 acgccggttt accctggggc gatgctctgc cccacctgtg gtgcacccga tacccgcgtc
     1441 atcgatagcc gacccgcaga gggtggccag tcgatccgtc gtcgtcgggt ttgtgagacc
     1501 tgcgaggcca ggttcaccac ctatgagcga ctcgagcccc agttgatggt gcgcaagcga
     1561 aacgggcgtc tggaggcctt ctcttcggcc aagctcgccg ccggcatctc agctgccttt
     1621 gccgaccgcc cggtctcggg ctcggctatc caatcgatcg ccgaagaggt cgagcactcg
     1681 ctgcgtcttc aggggtcaca agtgtcctcc gaggacatcg ggcatcaggt cctggagcgg
     1741 ttgcgcgccc tcgacgaggt ggcctatctc cgtttcgcct ctgtgtacaa ggacttccag
     1801 gatgccgccg acttcgagaa ggaagtcgcg gccctggagt catctgagta agcgaccgat
     1861 cagatcccga gccgcgtcca cttcagaatc tacgtcaccg gcgagattcg tcatcctggt
     1921 catcatcagc tccctgatcg cccccaggtc agactccagg gtctgcatcg cggtctcggc
     1981 agctcgttgg tcgttgctgc ggatcgcatc gagatagaca ccttggcggg tctcgaaact
     2041 cgagctgaac tcttccagtg acagggtcac ttcgtcggcc acgcctttgg gaagtgccga
     2101 cctgacgctg tcgaaatgtg tcctccactc agtgaagtcg agggcggcgg tagcgaggtc
     2161 ggtgaatgcc ggatcggtct ccaatgccgg tgcgaggagg atgggttcga gggcaccccg
     2221 gtaggcgagc ccgtctcgaa gctgtcgcga cacctcgaac gagagggagg ccacgtcggt
     2281 ggcaatcgat ctgatggccg tctcggatcc ggggagagtg ccggatgcgt caaacaggcc
     2341 ccgggcggca ttgtcgacat cgaagagatc ggtggcggtc aggttg
//
LOCUS       DATITW010000595         6292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_429953, whole genome shotgun sequence.
ACCESSION   DATITW010000595 DATITW010000000
VERSION     DATITW010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429953"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1714
                     /locus_tag="VLS86_08300"
     CDS             <1..1714
                     /locus_tag="VLS86_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSO50535.1"
                     /translation="YHFDAREPVARWLESDVATVSGNLCQALQLGDRLAEKRWPELLA
                     SGDPVARPVRKVMGKVGRRLARGGPDGQTGPLSAAVRIATSPGGVAADAGAWVELAGP
                     AEESWPATERQAFLALIATGEPGRVVFGWLEEVGWTRRSFAEWEAISAKPQLAAFHEH
                     PVDAHLWRTTDEMLALIREPDEIGAGILEDLAAAEELLLLSAFLHDIGKGVEGDHSEQ
                     GAVIAESFLRRAGFDPSTVEVVATAVRHHLLLARTALRRDIASPEVLDEVAEVVGGIN
                     ILQILYLLTIADSKATGRTMWSEWKETRLSQLYLRVAARLGVGEEPPVVHAEAVATLP
                     EVRFTPEEVNQHLAALPPDYAVLSEPGDVAWHLDVISRLADRTFLSVSESGEQVLVVG
                     RDRRGFLLAVSRAFAAHGIGVHDARLYTRVDGVVIDHFEVRDDRSGETVPPARWPKVE
                     ERMAALETEARDLVDVVRQRVAAYDTGPQLPVTVRPRPELTQPNTVIEVRAADRVGLL
                     VDIVEALFAEGLDLHLARIDTRGNQAIDVLHVSRSDAPVPSKHELDELCRRLEDRIRG
                     RLSG"
     gene            complement(1726..1962)
                     /locus_tag="VLS86_08305"
     CDS             complement(1726..1962)
                     /locus_tag="VLS86_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSO50536.1"
                     /translation="MAKPTKVTNQIRKLRFAAGEMTQGELADHVGVTRQTINAIEQGK
                     YSPSLEVAFRIADVFTIPLEEVFQYRPEDPERGP"
     gene            complement(1964..2383)
                     /locus_tag="VLS86_08310"
     CDS             complement(1964..2383)
                     /locus_tag="VLS86_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50537.1"
                     /translation="MTFQEKSAIVMTGALLLVYGSYFTVVLRWMAVSPVDEIAYQPLM
                     IVAIVPLVILATLSHIVLAIINPKEADAYDERDRAITIRAEQISAYVLAVGVFVGLVL
                     AMGGIAHFYIANSLLLAWVLAEITDRVTRIVLYRRGA"
     gene            complement(2486..3109)
                     /locus_tag="VLS86_08315"
     CDS             complement(2486..3109)
                     /locus_tag="VLS86_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50538.1"
                     /translation="MTQREKRIEILIVVVLGIAAFATAWSSYQASLWDGIQSSNYTQA
                     SGSRTNAAQQRTAANQFRLADLSVFENYIDATLNEDDDVADFYFQRFRDEFRVAYDAW
                     IAFDPLTNPDAPDSPFAMPEYQLSQDIEAERLEERADTLFADGEEANTISDIYTLTTL
                     LFAAALFFAAISERFEFPKARVVLLVMAGIGLVVGIGIAVGQPITTG"
     gene            complement(3305..4582)
                     /locus_tag="VLS86_08320"
     CDS             complement(3305..4582)
                     /locus_tag="VLS86_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSO50539.1"
                     /translation="MIGLTILAAVFICYSLVAARLDRWSITAPMVFVLTGTLIGLRAP
                     DWLGVMSDPEIVKHIAELTLALLLFADASTLRWAQLRHDGGLPLRLLLIGFPLTVVAG
                     LGAGAWLIPTAGWAAAALAASILAPTDAALGLGVFTNRSVPGRVRRALNVESGLNDGM
                     ATPLVTVFLAVLVAAEGSGTENWATEAVRDLAIAAVVAVLVGFGAGRLIAAAHQRQWT
                     SALSQRLAVLASGLLSYAGAVAAGGNGFVAAFVAGLVFAAASGGRLRESVEFTEDLAL
                     FASFLVWAIFGGLLVAPQLADGPSATSILYALLSLTLVRMVPVALALIGAGLRPASVL
                     FMGWFGPRGLASVVFTLIAFETLEHEGLASEPLIEIATWTVLLSVIAHGLTAGPLSRW
                     YGRRVADSPAEQGAAGHAGDPMPRALMSRHEAR"
     gene            4808..6256
                     /locus_tag="VLS86_08325"
     CDS             4808..6256
                     /locus_tag="VLS86_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50540.1"
                     /translation="MASDGDELSEIDRLIAENEQMKARLRRRGVWRRALTGLLVVLTS
                     LSVVATTFAVWVHQTVFDTDRFMETVSPALEDPALYDALGNKVSAQILDALDLEVRVT
                     ESLAALDEYISQALLDALDIDERARELLGRFDRPSLTALAPPIVEGLEERIDRRVSAF
                     FMSEQFTTRLPQLVRRAHEVTVALLRDDLAEVPNVYVAEGEVRLNLIPIIGEVLREAA
                     GDLRGFLPDIELPDVLSDVIAEGRQQIAAAIEARLPEDFGQLTLMSEQALLDAQSGAE
                     RLDRFVWGLLIVSIALIVVTILVSQTRRRTSIQIALGVVLGITIAAVIVRRVEAAIIE
                     GVVSPEGEVAARALLGGVVSNLRAIELIVALAAVVVAIIAYLAGGPAWLIRARARFDD
                     ITAQGADGSKLDRWVTRYFDLLRLTLIGLAVLVFFVVGFGWVSVLVISLVLALGLWLM
                     GAARARVGAVDQTAAHELVEGGSDVGRGQGAP"
BASE COUNT         1083 a         1982 c         2132 g         1095 t
ORIGIN      
        1 ctatcacttc gatgcccgtg agccggtcgc ccgctggctg gagagcgacg ttgcgacggt
       61 gagcggcaac ctgtgccagg cgctgcagct cggggatcgt ctcgccgaga agcgatggcc
      121 cgagcttctc gcctcggggg atcccgtggc gcggcccgtg cggaaagtca tgggcaaggt
      181 gggccggcgg ttggctcgtg gcgggcctga cggccagacc ggcccgctca gcgcggcggt
      241 gagaatcgcc accagcccgg gtggggtggc cgccgacgcc ggagcatggg ttgagctggc
      301 cggcccggcc gaggagtcat ggccggcgac cgagcgccag gcgtttctcg cactgatcgc
      361 caccggcgag ccgggccggg tggtgttcgg ctggctggag gaggtgggct ggacccgtcg
      421 tagctttgcc gagtgggagg cgatctcggc caagccgcag ctggcagcat tccatgagca
      481 cccggtggac gcccatctgt ggcggaccac cgatgagatg cttgctctga tccgcgagcc
      541 ggacgagatc ggggcgggga tactcgaaga cctggcagcg gccgaggagc ttctcctcct
      601 ctccgctttc ctccacgaca tcggcaaggg ggtcgagggt gaccactccg agcaaggagc
      661 cgtcatcgcc gaatcgtttc tgcgtcgtgc cggcttcgat cccagcaccg tcgaggtggt
      721 tgcgacggca gtgcgccacc atctcttgtt ggcgaggaca gccctgcgcc gcgacatcgc
      781 cagccccgag gtgctcgacg aggtggctga ggtggttggg gggatcaaca tcctccagat
      841 cctctatctg ctcaccatcg ccgattccaa ggcaaccggc cgcaccatgt ggtcggaatg
      901 gaaagagact cggctcagtc agctctacct ccgtgtcgcc gcccgactcg gggtgggaga
      961 ggagccaccg gtggtgcacg ccgaggccgt cgccaccctg cccgaggtga ggttcacccc
     1021 ggaggaggtg aaccaacatc tggcggccct gccccccgac tacgcggtgc tctccgagcc
     1081 gggtgatgtg gcctggcatc tcgatgtaat ctccagactc gctgaccgga cgtttctctc
     1141 tgtcagcgag tccggggagc aggtgctggt cgtcggtcgt gaccggaggg gattcctgct
     1201 ggcggtgtca cgggcgttcg ccgcacatgg aatcggagtt cacgacgcca ggctctacac
     1261 ccgggtcgac ggggtcgtca tcgaccactt cgaagtgcgt gacgaccgca gcggtgagac
     1321 ggtgcctccg gcccgatggc cgaaagtcga ggagaggatg gccgctctcg agaccgaggc
     1381 ccgcgacctg gtcgatgtcg tgaggcaacg ggtggccgca tacgacaccg gtccgcagct
     1441 gccggtgacg gtgcgtcccc gacctgagct gacccagccg aacactgtga tcgaggtccg
     1501 tgccgccgac cgggtgggtc tgctcgtcga catcgtcgag gcgctgtttg ccgaaggtct
     1561 cgacctccac ctggcgcgaa tcgacacccg aggcaaccag gcgatcgacg tcctccacgt
     1621 cagccggtcg gacgccccgg tccccagcaa gcacgagctg gatgagctgt gccgccgtct
     1681 cgaggaccgg atccggggcc ggctctccgg ataggtgagg gcggcctacg ggcctctttc
     1741 cgggtcttcc gggcggtact ggaacacctc ctcgaggggg atggtgaaca cgtcggcgat
     1801 ccggaacgcc acttcgaggg acggggagta ctttccctgt tcgatggcgt tgatggtctg
     1861 tcgggtgacg ccgacatggt cggccagctc cccctgggtc atctccccgg ctgcgaagcg
     1921 gagcttccgg atctggttgg tcactttggt cggcttcgcc acgtcaggct ccgcgccggt
     1981 agagcacgat cctggtcacg cgatcggtga tctcggccaa gacccacgcc agcaacaggg
     2041 agttggcgat gtagaagtgg gcgatcccgc ccatagccag caccagcccc acgaacactc
     2101 ctaccgctag gacgtaggcg ctgatctgtt cggcgcggat ggtgatcgca cgatcccttt
     2161 cgtcgtaggc atcggcctct ttcgggttga tgatggcgag cacgatgtgg ctgagtgtgg
     2221 ccaggatcac cagtgggacg atggccacga tcatcaatgg ctggtacgcg atctcgtcga
     2281 cagggctcac ggccatccac cgcaggacca ccgtgaagta ggacccgtag acgagcagca
     2341 acgcaccggt catgacgatg gcgcttttct cttggaacgt catggcggta ctcctgtcta
     2401 gaaagtttga cagggagcat agtaaagcaa actggacgtc gtgtaaagaa aacttaacag
     2461 gacttcggat gagaagccgg ggagatcagc cggtggtgat cggctgaccc accgcgatcc
     2521 cgatcccgac caccagaccg atcccggcca tcaccaggag cacgacccgg gccttgggga
     2581 actcgaatcg ctccgagatg gcagcaaaga acagagccgc cgcgaagagg agtgtggtga
     2641 gggtgtagat gtccgagatc gtgtttgcct cctccccatc ggcgaagagt gtgtcggccc
     2701 gttcctcgag acgctccgcc tcgatgtcct gtgacagctg gtactcgggc atggcgaagg
     2761 ggctgtcggg agcgtccggg ttggtcaagg gatcgaatgc gatccatgcg tcgtaggcca
     2821 ccctgaactc gtcgcggaac cgctggaagt agaagtcggc gacgtcatcg tcctcgttca
     2881 gggtggcgtc gatgtagttc tcgaagacac tcaggtcggc gagccggaac tggttggcgg
     2941 cggtccgctg ctgtgcggcg ttggtccggg agccagacgc ctgtgtgtag ttgctcgact
     3001 ggatcccgtc ccacagggac gcctgatagc tgctccacgc tgtggcgaaa gccgcgattc
     3061 ccaggaccac cacgatcagg atctcgatcc gcttctccct ttgagtcacc tccgacgatc
     3121 ctccgccctg cggcgcagcc gtcgaatcgc ccatgtcggt atcgctcatg ccccgccctc
     3181 actcgacgcc gggttctctc ctgtcgatcg tcccaatcat ccccgtggcg cgaccggacg
     3241 catcacccct ttgggttgaa accagttcgc tcgaccggca agggcccttg tggccatgcc
     3301 tccgtcatcg agcctcatgg cgcgacatca gcgctcgtgg catcgggtcc ccggcatggc
     3361 cggccgcccc ttgctccgcc ggggaatcgg caacgcggcg gccgtaccat cgagacaacg
     3421 gcccggccgt gagcccgtgg gcgatcaccg atagcaggac ggtccaggtg gcgatctcga
     3481 tcaatggctc cgaggccagg ccttcgtgtt cgagggtctc gaatgcgatc agcgtgaaca
     3541 caaccgaggc cagccccctg ggaccgaacc agcccatgaa caacacgctc gccggccgca
     3601 gtcccgctcc gatcagggcc agggccaccg ggaccatgcg aaccagggtg aggctcaaca
     3661 atgcatagag gatggacgta gccgagggtc catccgccaa ctggggtgcg acgagcagac
     3721 cgccgaatat ggcccatacg aggaacgagg caaagagagc cagatcctcg gtgaactcga
     3781 cggactccct gagtcggcct ccggatgcgg cagcaaacac gagaccggcc acgaaggcgg
     3841 caacgaaccc gtttccaccg gccgcgaccg cgccggcgta tgacagcaag cccgacgcga
     3901 gcactgcaag acgctgggac agtgcggagg tccactggcg ctggtgggcc gcagcgatca
     3961 gtcggccggc cccgaatccc acgaggacgg cgacgaccgc cgcgatggcc agatcgcgga
     4021 cggcctcggt cgcccagttc tcggtaccgg atccctctgc ggcgacgagg accgccagga
     4081 agacggtcac caatggggtg gccatcccgt cgttgagccc actctcgacg ttgagagcgc
     4141 gccggacgcg gcccggaacc gatcggttgg tgaagacgcc cagccccaga gcggcatcgg
     4201 tcggagccag gatgctcgcg gccaaggctg ccgccgccca acccgcggtc gggatcagcc
     4261 acgcgcctgc tccgaggccg gcgacgacgg tgagtgggaa cccgatgagc agaagcctga
     4321 ggggaaggcc tccgtcatgg cgaagctgcg cccatctgag ggtcgaggcg tcggcgaaga
     4381 gcagcagggc cagtgtcagc tcggctatgt gtttgacgat ctcggggtca ctcatcaccc
     4441 cgagccagtc gggtgcccgc aggcctatca gggttcccgt tagcacgaag accatgggag
     4501 cagtgatcga ccacctgtcc aggcgggcgg cgaccaggga gtagcagatg aacacggcgg
     4561 ccaggatggt gagcccgatc acgggtcaat cctgatacgc gtcggcccct tgcgaatcgc
     4621 ccaatggggg tgacgcgtgc cgcgccgacc cagacagatc gagcgcgaag gttggttcgg
     4681 tcgtctggca atcgtccgat gagggtgacg cccgttcagg caccccgacc taagttctga
     4741 ttgtcaggag ttgcctgctt cggaggtgga agtggccaaa ggagatgacg gcatacccga
     4801 tgatcgagtg gcgtccgacg gagatgagct ctccgagatc gatcggttga tcgccgagaa
     4861 cgagcagatg aaggcaaggc tccgacgtcg tggcgtctgg agacgagccc tcacgggtct
     4921 gctcgtcgtc ctgacgtctt tgagcgttgt cgccaccacc ttcgccgtct gggtgcacca
     4981 gacagtcttc gacaccgatc gattcatgga gacggtgtca cccgcactcg aggatccggc
     5041 cttgtacgac gccctcggga acaaggtgtc ggcgcagatc ctcgatgcgc tcgacctcga
     5101 ggtaagggtg accgaaagcc tcgccgcact cgacgagtac atctcacagg cgctcctcga
     5161 tgctcttgac atcgatgagc gagcccgaga gctgctcggc cgtttcgacc ggccttcgct
     5221 gaccgctctg gccccgccca tcgtcgaagg tctggaggag cgcatcgatc gcagggtgag
     5281 tgccttcttc atgtccgagc agttcacgac gaggctgccc cagctggtcc ggcgcgccca
     5341 cgaagtgacg gttgccctac tccgtgacga cctggccgag gtgcccaacg tctatgtcgc
     5401 cgagggcgag gtccgactga atctgattcc gatcatcggt gaggtcctgc gagaggctgc
     5461 cggcgatctt cggggctttc ttcccgatat cgagcttccc gatgtcctgt ccgacgtgat
     5521 tgccgagggc cgtcagcaga tcgctgctgc aatcgaggct cggcttcccg aggacttcgg
     5581 tcagttgacg ctgatgtccg aacaggctct cctcgacgcc caaagcggag cggagcgtct
     5641 cgaccggttt gtctggggtc tcctgatcgt gtcgattgcc ctgatcgtgg tcaccatcct
     5701 cgtgtcccag acgcggcggc gaacctcgat ccagatcgca ctgggcgttg tgctcggtat
     5761 cacgatcgcc gccgtgatcg tgcgccgggt ggaagcagcg atcatcgagg gggtagtcag
     5821 ccccgaaggt gaggtggctg cccgtgccct cctcgggggg gtcgtgtcca acctgcgagc
     5881 catcgagctc atcgtggcgc tggccgccgt cgtggtggcc atcattgcct atctggcggg
     5941 cgggcctgcc tggctgatca gagcgcgggc gagattcgac gacatcacgg ctcagggcgc
     6001 cgacgggagc aagctcgacc ggtgggtcac gaggtacttc gatctgctcc gcctgacgct
     6061 catcggtttg gcggttctcg tgttcttcgt ggtcggtttc ggatgggtct ctgtcctcgt
     6121 catcagtctg gtcctggcct tgggtctgtg gctcatggga gctgccaggg ctcgggttgg
     6181 cgccgttgac cagacggcag cgcatgaact ggtcgaggga ggctccgatg tcggtcgtgg
     6241 gcagggcgcg ccgtgagtgg cgagatgaga gcgagagacg aggtcgagat ga
//
LOCUS       DATITW010000596         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_269410, whole genome shotgun sequence.
ACCESSION   DATITW010000596 DATITW010000000
VERSION     DATITW010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269410"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VLS86_08330"
     CDS             <1..744
                     /locus_tag="VLS86_08330"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50541.1"
                     /translation="VNQIETLASNGFIVVAVDHTYGAVATRFGDGEVVPYDPAALPDV
                     EEVGEDAYAEASETLVTVFSEDLATVVNALEAGSGGPFGAIAEGADVTRLGLYGHSTG
                     GGAAIEFCLQDERCDAVLGLDPWVEPIPDRVIAVSATRPALYMRSDEWRGNENDAILR
                     GISERSTETTYWVGVEGASHNDFVIAPLISPFSTRFGFTGPIPAGRVIPIIDRYLVGF
                     FDVYLLETGSAALDTASFEEVSVEVIRPD"
     gene            complement(747..1340)
                     /locus_tag="VLS86_08335"
     CDS             complement(747..1340)
                     /locus_tag="VLS86_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSO50542.1"
                     /translation="MSGRVLRWVSLAVVTGAIVFAVVLASRFGSDPGLVESPLIGKPA
                     PGFDLAYLDGTGSVSLADLEGDIVVLNFFASWCFECREEHADLVATSEAFAGREVTFV
                     QIGYQEDPAASLAYLTEAGMSEFTRYLADPGSRTAIAYGVFGIPETFFIDPEGVVVGK
                     IIGEADALILGATIDSIQRGETPGQQITGDTQQRPDA"
     gene            complement(1337..2335)
                     /locus_tag="VLS86_08340"
     CDS             complement(1337..2335)
                     /locus_tag="VLS86_08340"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HSO50543.1"
                     /translation="MSERARSFVIGSAIVVMAVALLLLVSTRPATVDRVEQLGSRIKC
                     PVCQGESIANSPSPMAEDMMDLIAERVAAGHSDEQIVDEILSSYTGALLLDPPTSGGT
                     LALWLTPILAMAGGVAVIVWWKRHPGPERPREEVIGKRSRTRLLVGGLILVAAFAGIV
                     VVAASQLQDRAASASGVADLGSTDLSDVSNETMEAVIAANPQVTGMRLALAERYFDLG
                     DYRSAFPHYLVVAEDPDAVPEQVITALVRLGWMAYDGNGEVDTAVRLSGEALAIDPES
                     NAARYVMAQVQWCGAGETEQAVAIFEDLLEAPALDEETRAQVETDLRSAQAGEDCR"
     gene            complement(2328..>3136)
                     /locus_tag="VLS86_08345"
     CDS             complement(2328..>3136)
                     /locus_tag="VLS86_08345"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HSO50544.1"
                     /translation="FNRLAVPLSFLLLLTMGFGPVTPWKGAPPGLLWRRLRGPALVSL
                     TAGLVTALTVTRLGWVVLAVVLGTFVTSAIVWLLIELARNRAEKSGKPLWAEMATVVG
                     NDQPFWAGQLSHVGVVLVAIGIAFASNLPLHAEVEMSPGESVEFAGYTLRYDSPFRRS
                     EPNRVVQGANIEVLRDDRRLTTLEPSANFYPSMASAIVTPAVMTRPGGDLYLTLRRLD
                     AGTIELGLDTSPMIWLVWLGGLTAAAGGFWSLAARRVGRTVDAERATADV"
BASE COUNT          537 a         1021 c         1103 g          475 t
ORIGIN      
        1 gtcaatcaga tcgagaccct ggccagcaat ggtttcatcg tggtggcggt ggaccacacc
       61 tacggggcgg tggccactcg attcggagac ggagaggtgg tgccatacga ccccgcagcc
      121 ctgcccgacg tggaggaggt cggggaggac gcctacgccg aggcgagcga gaccctggtc
      181 acggtcttct ccgaggacct ggcgacggtg gtcaacgccc tcgaagcagg cagtggcggg
      241 ccctttggcg cgatcgccga aggtgctgat gtcaccaggc tgggccttta cggccattcg
      301 accggcgggg gggcggccat cgagttctgc ctccaggacg agcggtgcga tgccgtcctc
      361 gggctcgacc cctgggtgga gccgatcccg gatcgggtca tcgcagtctc cgcgaccaga
      421 cccgcgctct acatgcggtc cgacgaatgg aggggaaacg agaacgacgc catcctgcgc
      481 ggcatctcgg agcggtcgac cgagaccacc tattgggttg gggtcgaggg ggcgtcgcac
      541 aacgacttcg tgatcgcgcc gctgatctcg cccttctcca cccgcttcgg cttcaccggg
      601 ccgatcccgg cgggccgggt catcccgatc atcgatcgtt acctggtcgg gttcttcgat
      661 gtgtacttgc tcgagaccgg ttcggcggct ctggacaccg ccagcttcga agaggtgtcg
      721 gtggaggtca tccgcccgga ttgacttcag gcgtcgggtc gctgctgggt gtccccggtg
      781 atctgctggc ccggcgtctc cccgcgctgg atggaatcga tggtggcccc gaggatcagg
      841 gcatcggcct cgccgatgat cttgccgacg acgacaccct cggggtcgat gaagaaggtc
      901 tcggggatgc cgaacacccc gtaggcgatg gcggtccggc tcccgggatc ggcgagatag
      961 cgggtgaact ccgacatccc ggcctcggtg aggtaggcga gcgacgccgc cgggtcctcc
     1021 tggtaaccga tctggacgaa ggtgacctcg cggccggcga aggcctcgga ggtggcgacc
     1081 aggtctgcgt gctcctcacg acactcgaag caccatgacg cgaagaaatt gaggacgact
     1141 atgtcccctt cgagatcggc gagcgaaacc gaaccggtcc catccagata tgcgaggtcg
     1201 aagccgggcg ccggcttgcc gatcagtggg gattcgacga gtccgggatc ggagccaaaa
     1261 cgcgaggcga ggacgacggc gaacacgatg gcgccggtca ccacggcgag ggagacccag
     1321 cgcagcacgc gcccgctcat cggcaatcct ctccggcctg tgccgatcga aggtcggtct
     1381 cgacctgggc tctcgtctcc tcgtcgagag cgggtgcttc gaggagatcc tcgaagatgg
     1441 cgacggcctg ctccgtctcc ccggcgccgc accactgaac ctgggccatg acataccggg
     1501 cggcgttcga ctcgggatcg atggccagtg cctccccgct gagccggacc gcggtgtcga
     1561 cctcgccgtt gccgtcataa gccatccagc ccaggcgaac cagggcggtg atgacttgtt
     1621 ccggcacggc atctgggtct tcggcaacca ccagatagtg aggaaacgca gagcggtagt
     1681 cgcccaggtc gaagtagcgc tcggcgaggg cgagacgcat gccggtgact tgggggttgg
     1741 cggcgatcac ggcctccatc gtctcgttgc tcacatcgga gaggtccgta gagccgaggt
     1801 cggccacgcc cgaggcggat gcggccctgt cctgcagttg actggccgcc accacgacga
     1861 tcccggcgaa cgccgcgacc aggatcagcc cgcccaccag gagacgtgtg cggctcctct
     1921 tcccgatgac ctcctccctg ggtcgttccg ggccgggatg acgcttccac cagacgatca
     1981 cggccacccc cccggccatg gcgaggatcg gagtcagcca cagggcgagg gtgccgccac
     2041 tggtcggcgg gtcgagcagg agagcgccgg tgtacgagga gaggatctcg tcgacgatct
     2101 gctcgtccga gtggccggcc gccacccgtt ctgcgatcag gtccatcatg tcttctgcca
     2161 tcggcgacgg ggagttggcg atcgactcgc cctggcacac cgggcacttg atgcgggacc
     2221 cgagctgctc gacccggtcg acggtggctg gccgggtgga gacgaggagg agcagcgcca
     2281 ccgccatgac gacgatggcc gacccgatga cgaagctacg ggcccgctca gacatcggcg
     2341 gtggcccgct cggcgtcgac agtgcgaccg acgcgtctgg ccgccagcga ccagaagccg
     2401 ccggcggcag cggtgagccc gccaagccag accagccaga tcatcggcga ggtgtcgagc
     2461 ccgagctcga tcgtccccgc gtcgagcctg cgcagggtga gatagaggtc cccgccgggt
     2521 cgggtcatca ccgccggggt gacgatggcc gacgccatcg atgggtagaa gttcgcactg
     2581 ggctcgaggg tggtcaatcg gcgatcgtct cgcaggacct cgatgttcgc tccctggacc
     2641 acccggttcg gctcactgcg tcggaacggc gagtcgtagc gcagggtgta accggcgaac
     2701 tcgaccgact ccccggggga catctcgact tcggcgtgca gggggaggtt ggaggcgaag
     2761 gcgatcccga tcgcgaccag gaccaccccg acatggctca gctgcccggc ccagaacggt
     2821 tggtcgttgc cgacgaccgt ggccatctcg gcccacaacg gcttgcctga cttctcggcc
     2881 cggttccggg cgagctcgat caggagccag acgatggcac tggtcacgaa ggtcccgagg
     2941 actacggcga ggacgaccca gccgagccgg gtaacggtga gggcagtcac cagcccggcg
     3001 gtcagggaga ccagggccgg gccccgcagc cgccgccaga gcaggccggg cggggcaccc
     3061 ttccacgggg tcaccggccc gaaacccatg gtcagcagga ggagaaagga gagtgggaca
     3121 gcgagacgat tgaaga
//
LOCUS       DATITW010000597         1829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_229691, whole genome shotgun sequence.
ACCESSION   DATITW010000597 DATITW010000000
VERSION     DATITW010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229691"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1346)
                     /gene="thrC"
                     /locus_tag="VLS86_08350"
     CDS             complement(120..1346)
                     /gene="thrC"
                     /locus_tag="VLS86_08350"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HSO50545.1"
                     /translation="MKYVSTRGDAPELDFRGALLAGLASDGGLYVPEEIPSLPAGWQT
                     WDYRQAVTATLTLFGAEQVGPMVAEAASRFAHPEIAPVVEVGDRLVLELFWGPTLSFK
                     DHALQVLGRLLDREVEAPATIIGATSGDTGSAAIEACRGRDNLRILILFPDGLVSEFQ
                     RRQMTTVEDDNVIAIAVKGTFDDCQAMVKGAFRRHQGLLAVNSINWARIAAQTGYYVH
                     LAARMRKPFDVVVPTGNFGNVFSCWLAKRMGAPIDQITIANNANRGLADLVGTGSMAG
                     GEVVATLAPAMDIGVPSNLERFTADPGGEFRAGFAGDDEIRATIAEVDKSHGYLLDPH
                     TATAWFVGNDTLGARPQVVVGTAHPAKFAEAVEQSVGRKPTPPPGFENLSSRPERSVL
                     IAPEASELDRLIDKEW"
     gene            1392..1463
                     /locus_tag="VLS86_08355"
     tRNA            1392..1463
                     /locus_tag="VLS86_08355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1425..1427,aa:Arg,seq:ccg)
     gene            1552..>1829
                     /locus_tag="VLS86_08360"
     CDS             1552..>1829
                     /locus_tag="VLS86_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HSO50546.1"
                     /translation="MPRSIWKGAINFGLVTIPVGLYTATDNKTPKFKQLRKTDHSPIR
                     YKRVAEADGKEVEWDDIVKGYEFEKDRYVVFTDEELDSTHPESNKLVDV"
BASE COUNT          325 a          617 c          591 g          296 t
ORIGIN      
        1 gcaccattgc gtcacaatgg ggcatgcctc ggatgagggc tatgggtggg tcgccacgac
       61 gtcccttggc gccgtcggca agccgtatcg tagctgctcg tcgccattgc gcaaagcaat
      121 caccactctt tgtcgatcag ccggtcgagc tcggaggctt cgggggcaat gagaaccgac
      181 cgttccggcc ggctcgatag gttctcgaac cccggtggtg gcgtcggctt tcgccccacc
      241 gactgctcca ccgcctcggc gaacttggcc gggtgcgcgg tcccgaccac cacctggggc
      301 cgggcgccca gggtgtcgtt gcccacgaac catgcggtgg cggtgtgggg gtcgagaagg
      361 tagccatggc tcttgtcgac ctcggcgatc gtggccctga tctcgtcgtc accggcaaac
      421 ccggcccgga actcgccgcc aggatccgcg gtgaatcgct cgaggttgga tggcaccccg
      481 atgtccatcg ccggggccag ggtggcgacc acctctcccc ccgccatcga accggtgccg
      541 accagatcgg ccaggccgcg gttggcattg ttggcgatcg tgatctggtc gatcggtgcc
      601 cccatccgct tcgccagcca gcaggagaag acgttgccga agttaccggt ggggacgacc
      661 acgtcgaagg gcttcctcat gcgggccgcg agatggacgt agtacccggt ctgggctgcg
      721 atccgggccc agttgatcga gttcaccgcg agcaggccct gatgccggcg aaacgcccct
      781 ttcaccatcg cctggcagtc gtcgaaggtg cctttcaccg cgatcgcgat cacgttgtcg
      841 tcctcgacag tggtcatctg ccggcgctgg aactccgaga cgagcccgtc ggggaacaga
      901 atcaggatcc tcagattgtc ccgcccgcgg caggcctcga tcgccgccga gccggtgtcc
      961 cccgaggtgg cgccgatgat ggtggccggt gcctccacct cgcgatccaa caagcggccg
     1021 agcacctgga gggcgtgatc cttgaaggag agggtgggcc cccagaacaa ctcgaggacg
     1081 agacgatcgc cgacctcgac cacgggtgcg atctccgggt gggcgaagcg ggacgcagcc
     1141 tctgccacca tcgggcccac ttgctccgcc ccgaacaggg tgagggtggc ggtgaccgcc
     1201 tggcggtagt cccaggtctg ccagcctgca ggcagtgatg ggatctcctc gggcacgtag
     1261 agaccgccat ccgaggccag accggcgaga agggcgcccc ggaagtcgag ctcgggagca
     1321 tcaccccgtg tcgatacgta cttcaccccc cggagggtag gtgcgccggc ctcggtagga
     1381 ttggtcgaga agctcccgta gctcagggga tagagcaccg gcctccggag ccgggtgcgc
     1441 aggttcgaat cctgccggga gcgcccagcc accatggccg cggcccaccc cgacccagtc
     1501 tccccccttg cgacaccttt cttgtcacgc aggtcaaata gactccgtga catgccacgc
     1561 tcgatctgga aaggcgcgat caacttcggg ttggtcacca tcccggttgg cctctacacg
     1621 gccaccgaca acaagacccc caagttcaag cagctccgca agaccgacca ctcccccatc
     1681 cgctacaagc gcgtcgccga agccgacggc aaagaggtcg aatgggacga catcgtcaag
     1741 ggatacgaat tcgagaagga ccgctacgtg gtcttcaccg atgaggagct ggattcgacc
     1801 caccccgaga gcaacaagct ggtcgacgt
//
LOCUS       DATITW010000598         1741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_750320, whole genome shotgun sequence.
ACCESSION   DATITW010000598 DATITW010000000
VERSION     DATITW010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750320"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..343)
                     /locus_tag="VLS86_08365"
     CDS             complement(50..343)
                     /locus_tag="VLS86_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50547.1"
                     /translation="MSKFVFLYKGLWPEPTPEITDAWTSWFSEIGDSIVDSGNPFGPG
                     REVSDSASTDAPSGPQSLTGYSIVSADNIEEAEKLLANCPMITSVLVYEAMPM"
     gene            complement(340..699)
                     /locus_tag="VLS86_08370"
     CDS             complement(340..699)
                     /locus_tag="VLS86_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSO50548.1"
                     /translation="MRAGADQELDAVFDALANAHPRSIMQSLSRQPHSISQLASIEEM
                     SLPAIHKHIQALEDGNLVIKRKVGRTNYLALNRNPLRDLQKWVGEFNPHWGNERETLE
                     NYARYLGATHPEGKDDI"
     gene            1329..>1741
                     /locus_tag="VLS86_08375"
     CDS             1329..>1741
                     /locus_tag="VLS86_08375"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HSO50549.1"
                     /translation="MRYPEKLLSPGEEIKTQFRPHWSQIVREGVLSLLVLVLIILIAI
                     QDLTWKGWAIAGLALAWLLLVIAGFIRWWTTQHVITNERVIHRTGLISKKGKEIPLEV
                     INDVAFSQRAWERLFGTGDLLIESAGTHGQSRYSN"
BASE COUNT          297 a          523 c          582 g          339 t
ORIGIN      
        1 cgtgccgcgc gggtaagtcg ccggtgcccg ccgttgcaca ggaatccggt cacatgggca
       61 tggcctcgta gacgaggacg ctggtgatca tcgggcagtt ggccagcagc ttctcggcct
      121 cttcgatgtt gtccgcgctc acgatgctgt agcccgttag ggactgtggc ccggagggag
      181 cgtcggtgga cgcgctgtcg ctcacttccc ggcccgggcc gaacgggttg ccgctgtcga
      241 cgatgctgtc tccgatctcg ctgaaccaac tggtccaagc atctgtgatc tctggtgtcg
      301 gctctggcca aaggcctttg tacaggaaca cgaacttgct catatgtcgt ccttcccctc
      361 ggggtgggtc gcgcccaggt agcgggcata gttctcgagt gtctctctct cgtttcccca
      421 gtgggggttg aattccccca cccacttctg gaggtcgcgc aggggattcc tgttgagcgc
      481 gagatagttg gtacggccga cctttctctt gatcaccagg ttcccgtcct cgagcgcctg
      541 gatgtgcttg tggatcgcgg ggagagacat ctcctcgatg gaggccaact ggctgatcga
      601 atgcggctgc ctactgaggg actgcatgat ggatcgcggg tgggcgttgg cgagagcatc
      661 gaacacagca tccagttctt ggtccgcacc ggccctcata gttaaccttt tggtaaacta
      721 tcggaatccg ggcgcggatg caagacctgt ccagggccaa gccgagccat gcggccggct
      781 cgcgaaaacc cgatcatcgg tcgcgaaaaa caaacaggtc cacaggttgc caccgttgag
      841 cgctcggtct acttttcccg gcacgccgca ggggaaaccc ggtggcgggc ccggatgcag
      901 ggcacgacgt ggcgaaagcc gcatcgggac ctgggtgcgg gttacctgga gccaccggga
      961 aaccgagggc gccggggacg gtcggaagat cggacgggga tccttcggga tcgtcgagcg
     1021 gtagtccgag cggcgagtct tcgggctcgc aagatcttct cggagacgag gagaggcggt
     1081 cttcggaccg tcagcgatct tcggatcgca cctcgccaga ggcttcggtc actggcaaga
     1141 ctccgatcgg agttgggtcc ccggcccgac accgattggg cgagagtccc gagagggttg
     1201 aggcttcggc caaggctccc tcggggctgt ctcgttttcg gagcggctcg gcctggcgac
     1261 cgattcttcg gccggcggca agcctcctac aacggcagca ggcacccttc gatcggtagc
     1321 ctgcctccat gcgctaccca gagaagttgc tcagcccggg tgaggagatc aagacccagt
     1381 tccggccgca ctggtcgcag atcgtgcggg agggggtgtt gtcgctgctg gtcctggtgt
     1441 tgatcatcct gatcgcgatc caggatctca cctggaaagg gtgggcgatc gccggcctgg
     1501 cgctggcctg gctgctgctg gtgatcgccg gtttcatccg gtggtggacg acccagcacg
     1561 tcatcaccaa cgaacgggtc atccaccgga cggggctgat ctcgaagaag ggcaaggaaa
     1621 tacccctcga ggtgatcaac gatgtcgcct tcagccagag ggcatgggag cggctctttg
     1681 gcaccgggga ccttctcatc gagtcggccg gcacccacgg ccagtcccgg tacagcaaca
     1741 t
//
LOCUS       DATITW010000599         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_469882, whole genome shotgun sequence.
ACCESSION   DATITW010000599 DATITW010000000
VERSION     DATITW010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469882"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VLS86_08380"
     CDS             complement(<1..484)
                     /locus_tag="VLS86_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50550.1"
                     /translation="MVDYASDDSVPSRLYPNVGIDLNGDCWFLTSAVTTWMYVSVFAN
                     GEAILGFDPDPGPGAPVVITDPLPRCTSEPGSSVDPSTEAWQYVTHYIHPPPVPDVNP
                     APGDGVTGLPTFVGVAIPDVHDTQLGSATGVTLDIHIEVSGVDIDWGDSRVDSFPPDE
                     D"
     gene            complement(610..1194)
                     /locus_tag="VLS86_08385"
     CDS             complement(610..1194)
                     /locus_tag="VLS86_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50551.1"
                     /translation="MIRRLVAGLIVVGLAVGIWALWPRGDSDTTPTTLPVAVDTTLDS
                     TTLAPTTTSPPETTTTSAGHVVTSVEEAEAILRQLWFGWFEGIYNQDEERIREVVGTQ
                     AMLDSARSQFGVMEFQAAPDPTSIAISDLEILRSDQGCLALWAGLSASFRPGASEGVQ
                     VLRLFDDRWVFVGLWTYKEDLWEADCESQLESSF"
     gene            1304..1684
                     /locus_tag="VLS86_08390"
     CDS             1304..1684
                     /locus_tag="VLS86_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50552.1"
                     /translation="MADTSTERHPFRFLFKFLLFIGALAAVTKVVASKKKEYYGLTES
                     EARAKFEAKLGPRIGEEKASEIADQVIPRLKDSGVIKPDPMEQVVNDAKDVASEAVDK
                     VQDAAGAARNSMKDAAEKIEEKLD"
     gene            1736..>2182
                     /locus_tag="VLS86_08395"
     CDS             1736..>2182
                     /locus_tag="VLS86_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50553.1"
                     /translation="MRGLTPDQVEQALLRDEAIIAAARGRQMAVLAVADEMQLPNADG
                     CKSLTEWVAGRLDVSRETAYRLTSTKSRLADAPDLARDLSSGEISFDRAEALSRIPAR
                     DRADWHQGVDIQGLRRIAARHRRMSRFDDHESHASSHLMLQPNLDES"
BASE COUNT          450 a          673 c          682 g          377 t
ORIGIN      
        1 cgtcctcgtc cggcgggaag ctgtccactc tgctgtcgcc ccagtcgatg tcgacgccac
       61 tgacctcgat gtggatgtcg agagtcaccc cggtcgcact accgagttgg gtgtcatgga
      121 cgtcggggat cgccaccccc acgaaggtgg ggagcccggt caccccgtca ccgggtgccg
      181 ggttgacatc gggcacagga gggggatgga tgtagtgggt cacgtactgc catgcctccg
      241 tgctcggatc caccgagctg cccggctcgg aggtgcaacg gggcaacggg tcggtaatga
      301 ctacgggcgc tcccggcccc ggatcggggt cgaagccgag gatcgcttct ccattggcga
      361 acaccgagac atacatccag gtggtgaccg ccgaggtcaa gaaccagcaa tcgccgttga
      421 ggtcgatgcc gacgttggga tagagccgcg aggggaccga gtcgtccgag gcgtaatcga
      481 ccacatcccc acccgagatg cgacagcggg tgaccgtgcg ctcttggcca gaccagggat
      541 ccgtttcgct ggccacccaa cacgacgaga agtcctcagc aatcgccgga gccggaatgg
      601 agagggttac tagaaagagg attccagctg cgattcgcaa tctgcctccc acagatcttc
      661 cttgtatgtc cagagtccaa caaagaccca tcgatcatcg aagagcctga ggacctggac
      721 tccctccgac gctcctggcc tgaagcttgc actcagcccc gcccacaaag ccaggcaccc
      781 ttgatcggat ctcagaatct ccagatcaga gatggcgatg ctcgttggat caggcgccgc
      841 ctgaaactcc atcacaccga attgcgatct ggcactgtcc aacatcgcct gggtccccac
      901 cacctcccgg attcgctcct cgtcctggtt gtagatccct tcgaaccagc caaaccagag
      961 ctggcgcagg atggcctcgg cctcctcgac agaggtgact acgtgccccg ccgatgtcgt
     1021 ggtcgtttcc ggtggagagg tcgtggtggg ggcgagcgtc gtcgagtcga gggtcgtgtc
     1081 gaccgcgaca ggcagggtgg ttggcgtcgt gtccgaatct ccccgaggcc aaagggccca
     1141 gatcccgact gccagaccca ccacgatcag accggcgacc agccgtcgaa tcactgcatc
     1201 gtcgaaccta cgccccgcca ggagacaggt gaaggtctat gacccaggcc gcgaaaaatc
     1261 agcccgccgc attgggtatg gttgaagttg gaggtgcaga cagatggcag acacgtcgac
     1321 agagcgtcat ccgttccggt tcttgttcaa gttccttctg ttcatcgggg cgttggcggc
     1381 cgtgaccaag gtggtcgcct cgaagaagaa ggagtactac ggcctcacag aatccgaggc
     1441 gagggccaag ttcgaggcca agctcgggcc ccgcatcggt gaggagaagg ccagcgagat
     1501 cgccgaccag gtgatcccga ggctcaagga ctctggggtg atcaagcccg atccgatgga
     1561 acaggtcgtg aacgacgcca aggatgttgc gtccgaggcc gtggacaagg tgcaggacgc
     1621 ggccggcgcg gcccgcaatt cgatgaagga cgccgccgag aaaatcgaag aaaaactcga
     1681 ctgaattcgg ctcaaccggc cgaatttgtc acacctcggt cgtacgttgt gaactatgag
     1741 aggtttgacg cccgaccagg tcgaacaggc tctactccgg gacgaagcca tcattgccgc
     1801 cgcgcgagga aggcagatgg cagtcctggc cgtggccgat gagatgcagc tcccgaatgc
     1861 cgacggatgc aagagcctca cggagtgggt ggcgggacga ttggatgtct cgcgagagac
     1921 ggcctatcga ttgacctcga ccaaatcacg attggccgac gctcccgatc tggctcgcga
     1981 tctctcgagt ggtgagatca gcttcgaccg ggcggaggcc ctttcccgga tcccggctcg
     2041 cgatcgggcc gactggcacc aaggtgtcga tatccagggt cttcgccgaa tcgccgctcg
     2101 tcatcgtcgc atgagccggt tcgacgacca cgaatcccat gcatcgtcac atctgatgct
     2161 gcaacccaac ctggacgaat ca
//
LOCUS       DATITW010000600         1949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_189302, whole genome shotgun sequence.
ACCESSION   DATITW010000600 DATITW010000000
VERSION     DATITW010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189302"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VLS86_08400"
     CDS             complement(<1..567)
                     /locus_tag="VLS86_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSO50554.1"
                     /translation="MSDIVFEKPDPRDFLDIDSLLSDEEKLLRGTVRDYVQKEIVPHV
                     GDWWDEGKLPQTEMAKAFGAIGLLGMHLEGYGCAGTSATAYGLACLELEAGDSGIRSF
                     VSVQGSLAMFPIWKFGSEEQKETWLPRMASGEVIGCFGLTEPDSGSDPASMRTNAKRD
                     GSDWVLNGTNMWITNRSIANVAIVWARTD"
     gene            complement(600..1064)
                     /gene="purE"
                     /locus_tag="VLS86_08405"
     CDS             complement(600..1064)
                     /gene="purE"
                     /locus_tag="VLS86_08405"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HSO50555.1"
                     /translation="MSHKVAIVMGSGSDRPVMEKAGALLEQFGVEHVTRVLSAHKTPD
                     QAIEFAASAAEQGFGVIIAGAGKAAHLAGVMAAKSLLPVIGVPINASLDGIDALLSTV
                     QMPTGIPVATVAIDGATNAALLAVAILAINDEALRQKLADYRIDLANKTLAR"
     gene            1119..1721
                     /locus_tag="VLS86_08410"
     CDS             1119..1721
                     /locus_tag="VLS86_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HSO50556.1"
                     /translation="MFAIDALQPSLFGDTDPCPDPDFGGLERIDLDSLSWLDFTPGWL
                     AGDARLFDFLTETVEWQQPEVRMYEKMVRTPRLVGQVDGGSHPALESMVDLLSDRYRI
                     GMDRVSAGWYRIGADSGAWHGDRIARELPHSIVATVSLGGPRRFLIRPKGGGESMAFD
                     LGSGDLFVMGGACQRLWEHTVPKVASAPQRIALMFRHSYD"
     gene            complement(1712..>1949)
                     /locus_tag="VLS86_08415"
     CDS             complement(1712..>1949)
                     /locus_tag="VLS86_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HSO50557.1"
                     /translation="ETGEVIKGLDGVGEDVLVFHGGTKRDGKRLLVAGGRVLNMVGIG
                     PSIGHARERAYEAVSAVNWPGMQFRSDIAASTQS"
BASE COUNT          327 a          650 c          611 g          361 t
ORIGIN      
        1 atcggtcctc gcccacacga tcgccacgtt ggcgatcgag cggttggtga tccacatatt
       61 ggtcccgttg agcacccagt ccgacccgtc acgtttggca ttggtgcgca tcgaggccgg
      121 atcggagccg gagtcgggct cggtcagccc gaagcagccg atcacctcac cgcttgccat
      181 ccggggcagc caggtctcct tctgctcctc ggagccgaac ttccagatcg ggaacatcgc
      241 cagcgagccc tgcaccgaca cgaaggagcg gatcccggag tcaccggcct ccaactcgag
      301 acaggccagc ccgtaggcgg tggccgaggt cccggcacag ccataaccct cgagatgcat
      361 gccgaggagg ccaatggcac cgaaggcctt ggccatctcg gtctggggca gtttcccctc
      421 gtcccaccag tctccgacgt gggggacgat ctccttctgc acgtagtcac ggaccgtgcc
      481 ccggagcagc ttctcctcgt cggagagcag ggagtcgata tcgaggaagt cccgcggatc
      541 gggcttctcg aagacgatgt cactcatgga tggcgaatgg tatccgcaac ggcggatctc
      601 tatctggcca aggtcttgtt ggccaggtcg atccgataat cggccagctt ctggcggagg
      661 gcctcgtcgt tgatggcgag gatggcaacc gcgagcagcg ccgcgttcgt tgctccgtcg
      721 atggccaccg tggccaccgg gatcccggtc ggcatctgta cggtggagag aagggcgtcg
      781 atcccgtcca gggaggcatt gatcggcact ccgatcaccg ggagcaacga cttggcggcc
      841 atcacccccg cgaggtgggc tgccttgccc gccccggcga tgatcacccc gaagccctgc
      901 tcggcggcgc tggcggcgaa ctcgatcgcc tgatcgggag tcttgtgcgc cgaaagcacc
      961 ctcgtcacgt gttcgacccc gaattgttcg agcaaggcgc cggctttctc catgaccgga
     1021 cggtccgagc ccgagcccat gacgatggcg actttgtgag acatcaccgg gacggtaacc
     1081 gaatgaccca acttccaggc tcgactatca tcgaacgtat gttcgctatc gacgcactcc
     1141 aaccgtcatt gttcggggac accgatccct gcccggaccc cgatttcggt ggtctggagc
     1201 gaatcgatct cgactccctc agttggctcg acttcacccc gggatggctg gcgggtgacg
     1261 ccagattgtt cgacttcctc accgagaccg tcgaatggca gcagcccgag gtgcggatgt
     1321 acgagaaaat ggtgcgaacc ccgcgcctcg ttgggcaggt cgacggtggt tcccacccgg
     1381 cgctggagtc gatggtcgac cttctctcgg atcggtaccg gatcggaatg gaccgggtca
     1441 gcgccggctg gtatcggatc ggggccgact cgggcgcctg gcatggggac cgcattgccc
     1501 gggagttgcc tcactcgatc gtggccaccg tctcgctcgg cggtccacgg cggttcctga
     1561 tccgaccgaa gggtggcggc gagtcgatgg cattcgactt gggctccggt gacctcttcg
     1621 tgatgggggg cgcctgtcag cggctctggg agcacacggt gccgaaggtg gcctcagctc
     1681 cgcagcggat cgccctcatg ttccgtcaca gctacgactg agtgctggcg gcgatatcgc
     1741 ttcggaactg cattcccggc cagttcaccg ccgaaaccgc ttcataggcc ctctctcggg
     1801 catggccgat cgaaggtccg atccccacca tgttgagcac cctccccccg gcgaccaaca
     1861 gccgcttccc gtcgcgtttg gtaccgccgt ggaacaccag gacgtcctcg ccaaccccgt
     1921 cgaggccttt gatcacctcg cctgtctcc
//
LOCUS       DATITW010000601         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_509786, whole genome shotgun sequence.
ACCESSION   DATITW010000601 DATITW010000000
VERSION     DATITW010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509786"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2065)
                     /locus_tag="VLS86_08420"
     CDS             complement(<1..>2065)
                     /locus_tag="VLS86_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSO50558.1"
                     /translation="ALAFFYGDSELLLQVVVVSIAVTLVLFLFVPRLRPAFIAAISIP
                     ASIIATFTIMRMAGFTLNNLTMLGLSLSTGIVIDDAIIVLENIFRHIEEEGRPPMEAA
                     VTGAKEIVLAVMATTLSLVVIFLPVAFMGGLVGRFWNSFGLTATFAIMVSLLVAFTLT
                     PMLSARMLKSEKIGGGHGGGPQGHGSKATGLYAVLESGYDRLLVWCLNHRPAVLLLSL
                     GLLLSTVFIGRQMKPDFVVDDDMSEFEVIVETPPGSSLPRSDQIMQRLEGDLRTIPEV
                     VHLFTTVGVRGKDRANITDASIYVGLKHLRQRKRSQQVIMQEVRQTLKKYPDLRVSVQ
                     QISLISGGGFKQTPFNLVLRGPDLQRLDGYAQALIKRLTALHGFVDVDTSQAQRRPEV
                     QVWIDRKKASDLGIRAEDIALALRTMVGGEKVGFYREGGEQYDVRLRLMDDYRKDASV
                     ISALTVPAANGRLVKLNNVVTLNPGRAPAQIDRYAQERQITVQANLFRMALGEATAEA
                     DAAVKALALPSGYGTAYLGRGKLMAEAFYNFAIAFILSLLFIYMVLAAQFESFIHPVT
                     IMVSMFLSIPFGLVSLLAAGQTLNIYSIMGLFLLMGVVKKNAILQVDFTNVLRARGRP
                     RYEAQLEADRARLRPILMTTLAIIAGMLPVALGKGDGAASRASLATAVVGGQTLCLLI
                     TLLVTP"
BASE COUNT          406 a          626 c          681 g          352 t
ORIGIN      
        1 ccggggtcac cagcagcgta atgaggagac agagggtctg gccgccgaca acggcggtgg
       61 ccagcgaggc ccgtgaggcg gcaccgtcgc ccttgccgag agcgaccggg agcatgccgg
      121 cgatgatggc cagggtcgtc atgaggatcg gccggagtcg cgctcggtcg gcttcaagct
      181 gcgcctcgta gcgcggcctg ccgcgcgcgc ggagcacatt cgtgaagtct acctggagga
      241 tcgcgttctt cttcacaacc cccatgagga ggaagagccc catgatgctg tagatgttca
      301 gggtctggcc agccgccagg agactcacga ggccgaaggg gatcgagagg aacatcgaga
      361 ccatgatggt aaccgggtgg atgaaactct cgaactgggc ggcgagaacc atatagatga
      421 agaggaggga gaggataaac gcgatggcaa agttgtaaaa tgcctcggcc atcagcttgc
      481 cccggccgag gtacgcggtg ccgtagccgg aggggagcgc gagcgccttg acggcggcat
      541 cggcctcggc ggtcgcctcg cccagtgcca tccggaagag gttcgcctgg acggtgatct
      601 gtcgctcctg ggcgtaccga tcgatctggg cgggtgcacg tccagggttc agggtcacga
      661 cgttgtttaa tttcacgagc cggccgttcg cagcgggaac agtgagcgcc gatatgaccg
      721 acgcgtcctt ccggtagtcg tccatgagtc gcaggcggac gtcgtactgc tcgccgccct
      781 cccgatagaa cccgaccttc tcgcccccca ccatggtccg gagcgccagg gcgatgtcct
      841 cggccctgat ccccagatcc gacgccttct ttcggtcgat ccagacctgt acctctggcc
      901 gtcgctgggc ctggctggta tccacgtcca cgaacccatg gagagcagtc agtcgcttga
      961 tgagggcctg ggcgtacccg tccaacctct gcaggtccgg gccccgaaga accaggttga
     1021 aaggggtctg cttgaagccg ccacccgaga tcaggctgat ctgctgcacg ctgaccctga
     1081 ggtcggggta ttttttgagc gtctggcgga cctcctgcat gatcacctgc tgggagcgct
     1141 ttcgctgtct gaggtgcttc agccccacgt agatcgatgc atccgtgatg ttggcccggt
     1201 ccttgcccct gaccccgacg gtggtgaaca ggtgcactac ctcagggatc gttctgagat
     1261 cgccctcgag gcgctgcatg atctggtcgc tccgaggaag ggaggagccg ggtggggtct
     1321 ccacgatcac ctcgaactcg ctcatatcgt catcgacgac gaaatcgggc ttcatctgcc
     1381 gtccgatgaa gaccgtcgag agcagcagcc cgaggctcaa gaggaggacg gcgggcctgt
     1441 ggttgagaca ccagacgagc agccggtcgt agccgctctc cagaacagca tacaagccgg
     1501 ttgcctttga tccgtgcccc tgggggcccc cgccgtgccc tccaccgatc ttctcagact
     1561 tcaacatcct ggcgctgagc atcggggtca gggtaaacgc caccagtagc gagaccatga
     1621 tggcgaaggt ggccgtcaac ccgaagctgt tccagaaccg cccgaccagc cctcccatga
     1681 aggccaccgg aaggaagatg accaccaacg agagggtcgt ggccatgacg gccaagacga
     1741 tctccttcgc gccggtcacg gccgcctcca tcggggggcg cccttcctcc tcaatatggc
     1801 ggaagatgtt ctccagcacg atgatggcgt cgtcgatgac gatgccggtg gagagcgaga
     1861 ggccgagcat cgtgagattg ttcagtgtga agcccgccat ccgcataatg gtaaacgtcg
     1921 cgatgatcga ggcggggatc gagatggcgg cgatgaaggc gggccggagc cgagggacaa
     1981 agaggaacag gaccagggtg acggcgatcg acacgaccac gacctgcagg agaagctctg
     2041 aatcgccgta aaagaacgcc agtgc
//
LOCUS       DATITW010000602         1504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_109952, whole genome shotgun sequence.
ACCESSION   DATITW010000602 DATITW010000000
VERSION     DATITW010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109952"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..>1504)
                     /locus_tag="VLS86_08425"
     CDS             complement(108..>1504)
                     /locus_tag="VLS86_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50559.1"
                     /translation="LNALAGEEVSPARALRPTTEQPLAWVANEAREEVRPLLDWLGVE
                     RVVGHDRADVSGVAILDMPDVDSVRLEHRATVDALLPRIDAVAWVVDPEKYDDERLHE
                     YLRRLAPHAKRMRFIFNKADRLDPRQRQLLTDDFRRRLAGDGIDGATIDMVSALDGSG
                     FEELRAVLNRAAEGKAIVAAKLAIDAATEVDAIARAAGLEPGTSHSPLLPTKEREAAI
                     AAAVDGAMALVDPVGVSRQMEEAVLHRARRQGGSLLARILALMSLITGRKRRQADPGA
                     YLLEWRRRGSLGHVLNPVRSALVRAAGAVSPATRPAVLMTLGADDAETEVTRALDRAT
                     RQAARELTVPRSMLWPVVGFVQLLSGAVLLFAIAWYLLVIFGPGQLPVATFDVPYLGP
                     VPTPLLLITGALALSLLLGFVLNLHARWIGRRAGREVATRVGEAVSRSITTVGFGGLD
                     AVEAIRRRLASVAG"
BASE COUNT          281 a          529 c          477 g          217 t
ORIGIN      
        1 tgtgaaaaga cgaatccact gagcctgcca ggcccgtccc ctgcaagcgg aacgacttct
       61 cgcccgatcc tgcaacccga cacggggccg tgcccagatg atcctggcta gccggccact
      121 gacgcaaggc gacgacgaat cgcctcgacc gcatcgagac caccaaagcc gaccgtggtt
      181 atcgaccggg ataccgcctc tcccacccga gtcgctacct cacgccctgc ccgccggccg
      241 atccatctcg catggaggtt gagcacgaaa ccgaggagaa gactcaaggc gagcgcgccg
      301 gtgatgagga ggaggggcgt cggaaccggg cccagatagg gaacgtcgaa ggtagccacc
      361 ggcagctggc ccggcccgaa gatgacgagc aaataccagg cgatggcgaa aagaagaacc
      421 gcccccgaca gcaactgcac gaaaccgacc actggccaga gcatcgagcg gggcactgtt
      481 agttcgcggg cagcctgacg cgtcgcgcgg tcgagggccc tggtcacctc ggtttcggca
      541 tcgtctgctc cgagcgtcat caggaccgcg ggccgggtgg cgggcgaaac cgccccagcc
      601 gccctcacaa gggccgagcg aaccgggttg aggacatggc ccagactccc ccgccttcgc
      661 cactcgagca gataggcgcc agggtccgcc tgtcgccgct tccgtccggt gatcaacgac
      721 atgagtgcga ggatccgggc caggagcgag ccaccctggc ggcgggcccg atgcaacacc
      781 gcctcttcca tctggcggga aacccccacg gggtcgacca gtgccatagc cccatccact
      841 gcggcggcaa tcgccgcctc tctttccttg gtcgggagca acgggctgtg cgacgtgcct
      901 ggctccagcc cggccgctcg agcaattgca tccacctcgg tggcggcgtc gatagcgagc
      961 ttggccgcca cgatcgcctt tccctcggcg gcccggttta gcacagctcg caactcctcg
     1021 aacccgctgc catcgagagc cgagaccatg tcgatcgtgg ctccatcgat cccatcgccc
     1081 gccaaccggc ggcggaagtc atcggtcagc aattgtcgct gtctgggatc cagacggtcg
     1141 gccttgttga agatgaaccg catccgcttg gcgtggggcg ccagacggcg gagatactcg
     1201 tggaggcgct cgtcgtcgta cttctcgggg tcgaccaccc acgccaccgc gtcgatgcgg
     1261 ggcaggaggg catcaaccgt tgcccgatgt tcgagacgga cactgtccac gtcgggcatg
     1321 tccagtatgg cgaccccgga cacgtcggcc cgatcgtggc cgaccactcg ctcgacaccg
     1381 agccaatcga gcaagggacg gacctcctcc cgagcctcgt tggccaccca ggccaacggc
     1441 tgctcagtgg tgggtcgcag agcccgggcc gggctgacct cctcaccagc gagggcgttg
     1501 agca
//
LOCUS       DATITW010000603         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_550659, whole genome shotgun sequence.
ACCESSION   DATITW010000603 DATITW010000000
VERSION     DATITW010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550659"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VLS86_08430"
     CDS             <1..193
                     /locus_tag="VLS86_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HSO50560.1"
                     /translation="FVIAGNDDAIRAADLIAGAIADAAIEGRDLATNKAAKGDSAAEA
                     EPAEPDATSDVPGEAPVSG"
     gene            186..797
                     /gene="tsf"
                     /locus_tag="VLS86_08435"
     CDS             186..797
                     /gene="tsf"
                     /locus_tag="VLS86_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336547.1"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HSO50561.1"
                     /translation="MAEFGAKDIQKLRLDAGVGMMDAKQALTDADGDMEKAFELLRER
                     GQAKMAKRADREANQGTIGSYIHIQNDRPVMGVLVELACETDFVAKSQEFKQAADDIA
                     MHVSWGRPTWVTREEVDVETLAKEREIIQRQAVAEGKPEKVIDKIVDGRIESFYADNV
                     LYDQKFVNAEKFEGTVGDMVGQLGSKMGENISIRKVARVAVGE"
     gene            806..1522
                     /gene="pyrH"
                     /locus_tag="VLS86_08440"
     CDS             806..1522
                     /gene="pyrH"
                     /locus_tag="VLS86_08440"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HSO50562.1"
                     /translation="MTEPHARRVVLKLSGEAFADPEVGYGIDPVTVNRVAGEIAEAAS
                     SGIEIAVVVGGGNIFRGVSQAAKGMDPASADYMGMLATVINALALRDALEKAGVATRV
                     QSAITMQELAEPYIRLRAIRHLEKGRVVIFAAGTGNPFFTTDTTAALRAVEVGADSLL
                     KASKVDGIYDSDPKTNSDANRIEELSYMDFISRELRVMDTTAVTMCKEHGVPIVVFDM
                     ITPGNIVKAVSGEKIGTVVH"
     gene            1536..2087
                     /gene="frr"
                     /locus_tag="VLS86_08445"
     CDS             1536..2087
                     /gene="frr"
                     /locus_tag="VLS86_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HSO50563.1"
                     /translation="MISDLLTEAEHKMELAIEHVASEFSTVRTGRANPQLLQRISVEY
                     YGSPTPLQQLASISVPEPRLLVVQPFDKSIVGSIEKALQTSDLSLNPSNDGNVIRIAF
                     PPLTEERRKDLIKVVKNMAEEGRVAVRNIRRHSKTDMEALHGEISDDDIRRGEDELQK
                     LTDRYVEKIDRQLAHKEEELLEV"
     gene            2090..>3260
                     /locus_tag="VLS86_08450"
     CDS             2090..>3260
                     /locus_tag="VLS86_08450"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HSO50564.1"
                     /translation="MDDETPGPDQDKGQGPSPEEKPDEAEVFTLPLLSEDEDEDGEPS
                     PESPDDSADLGEASSEFIPRNLDDFSSDEYTDSTTQEYQGLAEEVSRAAEEQWEQQAV
                     SALVPGVGSGLVGFEDVTGASLVTEEDYEASEQALTSDLAMRVASALVIFGMFLGSLL
                     LGGWWFTTFVILVMVVGTGEFYATVRSDDYRPLALFGLLGVVFMGLGAHLSGAAAIAG
                     WAAAFALATVLFLSLSPRRQPLEDASVTIGGMAWVGLLSFAILIAAGPQPVAFILFVV
                     LVVAANDIGAFFVGRAFGSHVMAPQISPKKTWEGFFGGLIAGAVAASVVVTFPAWETI
                     GLAKGLVAAALIGLVSPIGDAVESMVKRTLGVKDMGSVLPGHGGILDRIDGFLFAV"
BASE COUNT          610 a         1016 c         1111 g          523 t
ORIGIN      
        1 cttcgtgatc gcgggcaacg acgacgcgat ccgggccgcc gatctcatcg ccggcgccat
       61 cgccgacgcc gcgatcgagg gacgtgacct ggccaccaac aaggctgcca agggggactc
      121 ggctgctgag gccgagccgg ccgagcccga tgccacctcc gacgtgcccg gggaggcgcc
      181 ggtcagtggc tgagttcggg gccaaggaca tccaaaagct ccggctcgac gccggggtgg
      241 ggatgatgga cgccaagcag gcgctcaccg acgccgacgg cgacatggag aaggccttcg
      301 agctgctgcg ggagcgagga caggccaaga tggcgaagcg ggccgaccgc gaggccaatc
      361 aggggaccat cggcagctac atccacatcc agaacgaccg cccggtgatg ggtgtgctgg
      421 tcgagctggc gtgtgagacc gacttcgtgg ccaagagcca ggagttcaag caggcagccg
      481 atgacatcgc gatgcacgtc agctgggggc ggcccacctg ggtgacacgt gaagaggtgg
      541 atgtggagac cctggccaag gagcgcgaga tcatccagcg gcaggcggtg gcggaaggca
      601 agccggagaa ggtgatcgac aagatcgtcg acggcaggat cgagtccttc tatgccgaca
      661 acgtgctcta tgaccagaag ttcgtcaacg cggagaagtt cgaaggcaca gtcggtgaca
      721 tggtcggcca gctcggatcg aaaatggggg agaacatctc gatccgcaag gtggctcgtg
      781 tcgccgtggg cgagtaggag gcgaagtgac cgaaccccac gcgagaaggg tggtgctcaa
      841 gctctcgggt gaggcgtttg ccgaccccga ggttggatac ggcatcgacc ccgtgaccgt
      901 caaccgggtc gcaggagaga tcgccgaggc ggcctcgtcc ggcatcgaga tcgccgtcgt
      961 cgtcggaggg ggcaacatct tccgcggggt gtcgcaagcc gccaagggaa tggacccggc
     1021 ctcggccgac tacatgggca tgctggccac cgtgatcaac gccctggcgc tgcgagatgc
     1081 cctggagaag gccggagtgg cgaccagggt gcagagcgcc atcaccatgc aggagctcgc
     1141 cgagccctac atacggctcc gcgccatccg ccatctggag aaaggcaggg tggtcatctt
     1201 cgccgccggg accggcaatc ccttcttcac gacggacacc accgccgccc tccgagcggt
     1261 cgaggtcggc gccgactccc tactcaaggc gagcaaggtc gacggcatct atgactcgga
     1321 ccccaagacc aacagcgacg ccaatcgcat cgaagagctg tcttacatgg atttcatctc
     1381 ccgggaactg agggtcatgg acaccaccgc cgttacgatg tgcaaggagc acggagtgcc
     1441 gattgtcgtg ttcgacatga tcactcctgg caacatcgtc aaggcggtga gcggggagaa
     1501 gatcggcacc gtggttcact gacgaggaga gaacagtgat cagcgacctc ctgaccgagg
     1561 ccgagcacaa gatggagttg gcaatcgagc atgtcgcctc ggagttctcg acggttcgca
     1621 ccggccgagc caacccccaa ctcctgcagc ggatatccgt ggagtactac gggtcgccca
     1681 cccccctcca gcaattggcc tcgatctcgg ttcccgagcc gcgtctcctc gtggtgcagc
     1741 ccttcgacaa gtcgatcgtt ggcagcatcg agaaggcgct tcagacctcg gacctcagcc
     1801 tcaacccgtc caacgacggg aacgtgattc gcatcgcctt cccgccgttg accgaggaac
     1861 gacgcaaaga cctgatcaaa gtcgtcaaga acatggccga ggagggacgg gtggcggttc
     1921 gcaacattcg ccgtcactcc aagaccgaca tggaggcgct ccacggcgag atctccgacg
     1981 atgacatccg gcggggcgag gacgagttgc agaagttgac ggatcgctac gtcgagaaga
     2041 tcgaccgtca gctggctcac aaagaagaag agctcctgga ggtctgatcg tggacgacga
     2101 gacaccgggc ccggatcagg acaagggcca gggaccctct cccgaagaga agccggacga
     2161 ggccgaagtg ttcaccctcc ctctcctctc cgaggacgag gacgaggacg gggagccgtc
     2221 cccggaaagc cccgatgatt cagccgatct cggcgaggcg agctccgagt tcatcccacg
     2281 caacctcgac gatttttcct ccgacgagta caccgactcg accacccagg agtaccaggg
     2341 gctggccgag gaggtcagcc gagccgccga ggagcagtgg gagcagcaag cggtgtcggc
     2401 actggtcccc ggagtgggca gcggcctggt cgggttcgag gatgtaaccg gggcctcgct
     2461 ggtgaccgaa gaggactacg aggccagcga gcaggccctc accagcgatc tggcgatgcg
     2521 cgtcgcctcg gcactcgtca tcttcggcat gttcctcggg agtctcctcc tgggcgggtg
     2581 gtggttcacc acgttcgtga tcctggtgat ggtggtcggg accggggagt tctatgccac
     2641 ggtccgctcc gacgactacc ggccgctcgc gctgttcggc ttgctcggcg tcgtcttcat
     2701 gggtctcggc gcgcacctgt cgggcgccgc ggccatcgcc gggtgggcgg ccgcgttcgc
     2761 cctggccacg gtgctgttcc tctccctgtc gccacgtcgt cagccgctcg aggacgcctc
     2821 ggtcaccatc gggggaatgg catgggtcgg ccttctttcc tttgcgatcc tgatcgcggc
     2881 cggcccacaa ccggtggcct tcatcctgtt cgtggtgttg gtggtcgccg ccaatgacat
     2941 cggcgccttc ttcgtcggaa gggccttcgg ctcccacgtc atggcgccac agatctcccc
     3001 gaagaagacc tgggagggct tcttcggggg tctgatcgcc ggagctgtcg ccgcctcggt
     3061 cgtggtcacg ttcccggcgt gggagacgat cgggttggcc aagggcctgg tcgccgcagc
     3121 gctgatcggc ctggtgagcc cgatcggcga cgcggtcgag tcgatggtca agcggacgct
     3181 gggggtgaag gacatgggat cggtgctgcc cggccacggc gggatcctcg accggatcga
     3241 tgggttcttg ttcgcggtcc
//
LOCUS       DATITW010000604         4353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_69970, whole genome shotgun sequence.
ACCESSION   DATITW010000604 DATITW010000000
VERSION     DATITW010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69970"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..609)
                     /locus_tag="VLS86_08455"
     CDS             complement(49..609)
                     /locus_tag="VLS86_08455"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HSO50565.1"
                     /translation="MDLAELLAGSPEHAAPMLLGSYLVSEVGGALVKARVTEVEAYKG
                     SDDPASHAFRGRTRRNGSMFERPGTLYVYRSYGVHWCGNVAAGPEGIGWGILFRGAEI
                     VEGLGVARSRRGRDDGLANGPGKLTQALGIDHSHDGTYLLDPSSPLRIESGSRPAMVM
                     ATPRVGITTAEDRPWRFVEITAASGG"
     gene            complement(611..2998)
                     /gene="pheT"
                     /locus_tag="VLS86_08460"
     CDS             complement(611..2998)
                     /gene="pheT"
                     /locus_tag="VLS86_08460"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HSO50566.1"
                     /translation="MRVPMRWLGEFINLPTSDPAELSTVLAMLGHEVDGYETLEAGWS
                     SVVVGRVEKIAAHPDADKIRVCQVDTGSGPEQIICGAWNFSEGAMVAVARPGTVLPGE
                     FEIGQRTIRGVESNGMICSEKELGLGEDHTGILVLEGQPPLGTELVELLELPDVVFDL
                     AITPNRPDAMSLLGIARDLAAHYQVEHRVPPRPLPTVPGSSGIAVEIGDPIGCRRFTA
                     RLIRDVTVGRSPLWVRHRLNKAGIRAISNVVDATNYVMLELGHPLHAFDADSIAGGRL
                     RVKRADQGETLVTLDDVERNLSPEDLIIYDDEGPTSMSGTMGGARSEVSAETVNVLME
                     AASWDPPTIMYMSRRHGLRSEASTRFERGVDPNLTDEADARAAAMVVEMAGGTVAQEV
                     IDEIPTPIRPAEIELDIGEVERVLGPGFTSDGVADILTRLGMAVEAGAPLRVEVPTYR
                     PDLTRSADLIEEVARIHGYDRFAATVPLGAAGSLTQEQKRLRALSATLVGVGLNQAVT
                     LPFVNVEDLRRLGRDDSSRLLQVKNPLREEEGTLRPTMIPGLLNAARFNLSHGAQDVS
                     LFEIARVFTAVPWPEDPRLPTQVDRLAWVMVGAVGPQILGSDATLADVGVSLSLIRHV
                     LAMLGHDDVELEADAPAGYHQGRSAAVRLSGSVIGHAGELSPRAVREFDLVGRVAIAE
                     IELAPILAPPGLAIARSPSVFPQVDFDLSFLLPSGLEVAEVIRASVDAGVGLVESARV
                     FDEFRGPGVEEGKRAVAIRYRLRADDRTLTNEEVSPVRAAMIAAAEGLGAQLRGV"
     gene            complement(2995..4044)
                     /gene="pheS"
                     /locus_tag="VLS86_08465"
     CDS             complement(2995..4044)
                     /gene="pheS"
                     /locus_tag="VLS86_08465"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HSO50567.1"
                     /translation="MDPRSALDGVLAEAPTAIDAASSLDELDQVESSLLGKSSTIGQV
                     RRSMGALDPGERPLFGAALNQATETIQELLSRRRSQLEIEDEARRLSDGRIDVTLESH
                     SFPRGSLHLIQQTIDEVVDIFVGLGYRVADGPEAELGWYNFDALNTPPTHPSRLESDT
                     VYLDYGDPADEVLLRTQTSPVQVRWMEDNQPPVYVVVPGKVYRKETTDATHLPVFHQI
                     EGLAVDESIRFSDLKGTLAHFSREFFGPTTQVRLRPHFFPFTEPSAELDVSCFVCDGG
                     VPGCRVCGGAGWLEMLGCGMVDPHVLEAVGYDPSRLSGFAFGMGVERMAMVRHGINDI
                     RHFVDNDLRFLRQFR"
     gene            complement(4057..>4353)
                     /locus_tag="VLS86_08470"
     CDS             complement(4057..>4353)
                     /locus_tag="VLS86_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HSO50568.1"
                     /translation="GHFRAAIRAVESLADLEGYVPIAAVVDGGVPPSRLDPGRYALLV
                     GEEASGLPPEVVEGSQLKVTIPMPGGQESLNAAMAAGVIVYELSKPEREGQGQV"
BASE COUNT          725 a         1512 c         1427 g          689 t
ORIGIN      
        1 gggagtcgtc atcgatggtg ggagggtaaa cgcggcggga ccgctcccct agccaccgct
       61 ggcagcggtg atctcgacga aacgccacgg ccggtcctcg gctgtggtga tcccgactcg
      121 gggcgtggcc atgaccatcg ccggtcgcga ccccgattcg attcgcagtg gagacgacgg
      181 gtcgagcaga taggtcccgt catgggagtg atcgatcccg agggcctggg tcagcttgcc
      241 ggggccgttg gccagcccgt cgtcacggcc ccgcctcgat cgggccacac cgagcccttc
      301 gacgatctcg gccccgcgga acaagatccc ccagccgatc ccctcgggcc cggcggcgac
      361 attcccacac caatggaccc cgtacgagcg atagacatag agggtgccgg gacgctcgaa
      421 catggacccg ttgcgccggg tccgaccccg gaaggcgtgg gacgccggat cgtcggagcc
      481 cttgtaggcc tcgacttcgg tgactcgcgc cttgaccagg gcgccaccga cctcggacac
      541 cagataggag ccgagaagca tcggggcagc gtgttccggg cttcccgcca acagctcggc
      601 gagatccacc tcagacgccc cgcagctggg ctccgagccc ttcggcggcc gcgatcatgg
      661 ccgcgcgaac cggggacacc tcttcgttgg tgagcgtccg atcgtcggct cgcagtcggt
      721 atcggatcgc caccgcccgt ttcccctcct ctactcccgg gccgcgaaac tcgtcgaaga
      781 cccgcgccga ctccaccagg cccacgcctg cgtcgacact ggcgcggatc acctccgcca
      841 cctcgagacc ggaggggagc aggaaggaga ggtcgaagtc gacctggggg aacaccgacg
      901 gggagcgggc gatggcgagc ccgggcgggg ccaggatcgg ggcgagctcg atctcggcga
      961 ttgccaccct cccgaccagg tcgaactcgc ggacggcgcg tgggctcagc tcaccggcat
     1021 gtccgatcac ggagccggac aacctgaccg cggccgacct tccctggtga tacccggcgg
     1081 gcgcatcggc ctcgagctcg acgtcgtcgt ggcccagcat cgccaggacg tgcctgatca
     1141 gcgacagcga taccccgacg tcggccaggg tggcgtcgga gcccaggatc tgggggccga
     1201 ctgccccaac catcacccag gccagccggt cgacctgtgt cggaagccgg gggtcctcgg
     1261 gccagggcac ggcggtgaac acgcgggcga tctcgaacag cgagacgtcc tgggccccgt
     1321 gcgacaggtt gaatcgggcg gcgttgagca gtccggggat catggtgggg cgcaacgtcc
     1381 cttcctcttc ccgcagcggg ttcttgacct gcaacagccg ggaagaatca tcccggccca
     1441 gccgtctcag gtcctcgacg ttgacgaagg ggagcgtcac cgcctgattg agacccaccc
     1501 cgaccagggt cgcgctcagc gcccggagac gcttctgctc ctgggtcaaa ctgcctgcgg
     1561 cgcccagggg cacggtggcc gcgaaccggt cataaccgtg gatacgggcg acctcttcga
     1621 tcaggtcggc tgatcgggtc aggtccgggc gataggtggg cacctcgacc cgaagcggcg
     1681 cgccggcttc gaccgccatc cccagtctgg tcaggatgtc ggcgacaccg tcgctggtga
     1741 accccgggcc aaggacgcgc tcgacttctc cgatgtccag ctcgatctcc gccgggcgga
     1801 tcggggtcgg gatctcgtcg atcacctctt gggccacggt gccgccggcc atctccacca
     1861 ccattgcggc cgccctggcg tccgcctcat cggtgaggtt ggggtccact cccctttcga
     1921 acctggtcga ggcctcggat ctgagcccgt gtcgcctgga catgtacatg atcgtgggcg
     1981 ggtcccacga ggcggcctcc atcaggacgt tgacggtctc cgcggacact tccgatctcg
     2041 ccccacccat cgtccccgac atcgaggtcg ggccctcgtc gtcgtagatg atcaggtcct
     2101 ccggagacag attccgctcc acgtcgtcga gggtcaccaa tgtctcgccc tggtcggcgc
     2161 gcttcaccct gagccgacca cctgcgatcg agtcggcgtc gaaggcatga agcgggtgac
     2221 ccagttcgag catcacgtag ttggtggcgt ccaccacgtt ggagatcgcc cggatcccgg
     2281 ccttgttcaa ccggtgcctc acccacagcg gggacctgcc cacggtcacg tctcgaatca
     2341 gcctggccgt gaagcgacga cacccgatcg gatcaccgat ctcgaccgcg atccccgagg
     2401 agccgggaac ggtggggagc ggtcggggag ggacccggtg ctccacctgg tagtgagcag
     2461 ccagatcgcg ggcaatcccc agcaaggaca tcgcgtcggg ccggttgggg gtgatggcca
     2521 ggtcgaagac cacatcggga agctcgagca gctcgaccag ctcggtgccc agcggtggtt
     2581 gaccttccaa caccaggatg ccggtgtggt cctcgcccag ccccagctcc ttctccgagc
     2641 agatcatccc gttcgactcc acgccccgga tcgtcctctg cccgatctcg aactcgccgg
     2701 gcaggacggt tccgggccgg gcgacggcga ccatcgctcc ctcggagaag ttccaggcgc
     2761 cacaaatgat ctgctccggg cccgagccgg tgtcgacctg gcacacccgg atcttgtccg
     2821 cgtcggggtg ggcggcgatc ttctccaccc tgcccacgac cacactcgac catccggcct
     2881 cgagggtctc gtacccgtcc acctcgtgac cgagcatggc cagcacggtg gagagctcag
     2941 ccgggtcgct cgtgggcagg ttgatgaact ccccgagcca gcgcatgggg actctcatcg
     3001 gaactgccgc agaaatcgga ggtcattgtc cacgaagtgc ctgatgtcgt tgatgccgtg
     3061 ccggaccatc gccatccgct ccacgcccat cccgaaggca aaacccgaca gccgtgacgg
     3121 gtcgtatccg accgcctcga ggacatgggg atcgaccatc ccgcagccga gcatctccag
     3181 ccatcccgcc cctccgcaca ccctgcaccc gggcacgccg ccgtcacaga cgaagcaaga
     3241 gacgtccagc tcggcgctgg gctcggtgaa cggaaagaag tggggtcgga gcctgacctg
     3301 ggtcgtcggc ccgaagaact cgcgcgagaa atgggccagg gttcccttca ggtcggagaa
     3361 cctaatggat tcgtccacgg ccaagccctc gatctggtgg aagaccggga gatgtgtggc
     3421 atcggtcgtc tcctttcggt agaccttgcc cgggaccacg acatagacag gtggttggtt
     3481 gtcctccatc catctcacct ggaccggtga ggtctgtgtc cggagcagca cctcgtcggc
     3541 agggtcgcca tagtcgagat agacggtgtc cgactcgagc cggctggggt gggtcggcgg
     3601 ggtgttcagc gcgtcgaagt tgtaccagcc aagctcggct tccggaccgt cggcgacccg
     3661 gtatccgagc ccgacgaaga tgtcgacgac ctcatcgatc gtctgttgga tcagatgcag
     3721 cgagccccga gggaagctat gggattcgag cgtcacgtcg atgcgaccgt cgctgagccg
     3781 ccgggcctcg tcctcgatct cgagttggga ccggcggcgg gagagaagct cctgaatcgt
     3841 ctccgtggcc tggttgagcg cggcgccgaa cagcggtcgc tcccccgggt cgagggcccc
     3901 catcgaccgt ctgacctggc cgatggtcga gctcttgccc aacaaggagc tctccacctg
     3961 gtcgagctcg tcgagagacg acgcggcgtc gatcgccgtc ggcgcctcag ccaacacacc
     4021 gtcgagcgcc gatctgggat ccatgtcggc ggcaggttat acctgcccct gcccctctct
     4081 ttcgggtttc gagagctcgt agacgatcac tcccgccgcc atggcagcgt tgaggctctc
     4141 ctgcccgccg ggcatcggga tggtcacctt cagctgcgag ccctcgacca cctcaggggg
     4201 caagcccgag gcctcctccc caaccaagag ggcgtagcgg cccggatcga gacgtgaggg
     4261 aggcacgcca ccgtcgacca ccgcggcaat cggcacgtat ccctccaggt cggccaggga
     4321 ctcgacggcg cggatcgcgg cgcgaaagtg gcc
//
LOCUS       DATITW010000605         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_470100, whole genome shotgun sequence.
ACCESSION   DATITW010000605 DATITW010000000
VERSION     DATITW010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470100"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VLS86_08475"
     CDS             <1..255
                     /locus_tag="VLS86_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50569.1"
                     /translation="TVRFEPESPADKTMNLERMRQLTSMQAFPGGDPDGGGTVWLWAV
                     PRALSTLVVIGLGAFLLSGLTKPGKHERRVVTRRLSISAP"
     gene            complement(345..>2693)
                     /locus_tag="VLS86_08480"
     CDS             complement(345..>2693)
                     /locus_tag="VLS86_08480"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HSO50570.1"
                     /translation="TSFPGNSVITTTGLIDAVVYGTSDPDDPELLALLNPGQPQVDED
                     GGGDGAAQSSQRCPNGSGGARNSESFAQFAPTIGAENTCFVAPPAPVPYKIHEIQGAG
                     ASSPLDGTQVIIEGIVVGDFQGNAALRGFHLQEEDVDADANPLTSEGIFVFDASFGVD
                     VVVGDLVEVIGTVDEFNGLTEITSVTSLVVVSGGNTVTPATIVLPVSATGDLEAFEGM
                     SVRIPQDLFISEFFNFDRFGEIVLTTERQFQPTARHEPGSPEAAQLAIANQLGRITLD
                     DGRNSQNPDPAIHPNGEVFDLDNRFRAGDRVSNVVGVLDFAFGLYRIHPTEGADFTAL
                     NRRPKRPPQVGGSLEVATFNVLNYFSTIDTGVFICGPLADQECRGADDDDEFTRQRDK
                     IISAIAKLDADVVGLMEIENHVTDAALADLVSGLNARMGAGTYQYIGEGSIGPDAIRV
                     AIIYKPKRVIPLGADAVLDTDAFLDPNGLGGAQNRPAQAQAFVEKKTGEIFNVVVNHL
                     KSKGSACGPGDDDAVQGNCNLTRTLAAQVLADWLATDPTGSGDGDYLIIGDLNSYDKE
                     DPIDALTLGADDTTGTTDDFSDLLLEFEGEDAYSYLFDGQLGYLDYAMANQSLLAQVT
                     GAGVWHINADEPDIIDYDTGFKQDAQDALYAPDPYRSSDHDAVVVGLRLDQTPPRVKA
                     DLDQISVRHNRGWFRIDFSCKDKIDPRPTCEADLNGIEVRDGQKVYLIESSGAPWALE
                     VGRTLYLKDDTFLLTVTGTDESGNSATALAEPDFGSRRHGWH"
BASE COUNT          448 a          856 c          880 g          509 t
ORIGIN      
        1 acggtccggt tcgagcccga atccccggca gacaagacga tgaacctgga gcggatgcgc
       61 cagttgacgt cgatgcaggc cttcccgggg ggcgatcccg acggcggggg aacggtttgg
      121 ctgtgggcag tcccgcgtgc gctttccact ttggtcgtga tcggcctcgg agccttcctc
      181 ctctcggggt tgaccaagcc cggcaagcac gagcgacggg tggtcacccg ccggctttcc
      241 atctccgccc cctgaccgct gccggcctca cacctgagac gggccaaccg acgagcggga
      301 acgcctggcc tcggctcccg ctcgtcaatt gtccccctcc acgatcaatg ccagccgtgc
      361 ctccggctcc caaaatcggg ctcggcgaga gcagttgcgc tgttgccgct ctcatcggtg
      421 ccggtgacgg tgagcaggaa cgtgtcgtcc ttcaggtaca gggtgcgccc gacctcgagg
      481 gcccatgggg ctcctgacga ctcgatcaag tacacctttt gcccgtcgcg aacttcgatg
      541 ccgttcaggt ctgcctcaca cgtcggccgc gggtcgatct tgtccttgca ggagaagtcg
      601 atccggaacc aacccctgtt gtgtcgcacc gagatctggt ccaggtccgc cttgacccgt
      661 ggcggggtct gatcgagcct gagaccgacg accaccgcgt cgtgatcgga ggaccggtag
      721 ggatccggcg cgtacagcgc atcctgggcg tcctgcttga acccggtgtc gtagtcgatg
      781 atgtcgggct cgtcggcgtt gatgtgccaa accccggccc cggtcacctg tgccagaagc
      841 gactgattgg ccatcgcgta gtcgagatag cccagttgcc cgtcgaagag ataggagtag
      901 gcatcctctc cttcgaactc gagcaagagg tcgctgaagt catcggtcgt ccctgtggtg
      961 tcatccgctc ccagggtgag agcgtcgatc ggatcctcct tgtcgtacga gttgaggtcg
     1021 ccgatgatca gatagtcacc atcaccgctg ccggtgggat ccgtggccag ccaatcggcc
     1081 aacacctgcg cggccagggt cctggtgaga ttgcagttgc cctggaccgc gtcgtcgtcg
     1141 ccgggcccac aagccgagcc cttcgatttc agatggttga ccacgacatt gaagatctca
     1201 ccggtcttct tctcgacgaa cgcctgagcc tgcgccggcc ggttctgggc gccgcccagg
     1261 ccgttcggat ccaggaacgc atcggtgtcg aggaccgcat ccgcacccag ggggatcacc
     1321 cgctttggct tgtagatgat ggcgactcga atcgcatccg ggccgatcga gccctctccg
     1381 atgtactggt aggtgcccgc gcccatcctg gcgttgagcc cggagaccag atcggcgaga
     1441 gcggcatcgg tgacgtggtt ctcgatctcc atcaaaccca ccacgtcggc atcgagcttg
     1501 gcgatcgccg agatgatctt gtcccgttgc cgggtgaact cgtcgtcgtc gtcggctcca
     1561 cggcactcct ggtcggccag aggtccacag atgaaaactc cggtgtcgat cgtggagaag
     1621 tagttcaaga cgttgaaggt ggcgacttcg aggctgcctc ccacctgcgg cggtcgcttc
     1681 gggcgccggt tcaacgcagt gaagtcggct ccctcggtcg ggtggatccg gtagaggccg
     1741 aaggcgaagt cgagcacacc gacgacattg gacacccggt ccccggcgcg gaaacggttg
     1801 tccaggtcga acacctcacc attggggtgg atcgccgggt ccgggttctg gctgtttcgc
     1861 ccatcgtcga gggtgatccg tcccaactgg ttggcgatcg ccagctgtgc cgcctcgggt
     1921 gagccgggct cgtgccgcgc ggtgggttgg aactggcgct cggtggtcag cacgatctcg
     1981 ccgaagcggt cgaagttgaa gaactcggag atgaaaaggt cttgaggaat ccgaaccgac
     2041 atcccttcga aggcctcgag atctccggtc gccgagaccg ggagcacgat ggtggccggg
     2101 gtcactgtgt tgccgccgga gacgacgacc aaggacgtca ccgaggtgat ctcggtcaga
     2161 ccgttgaact cgtcgacggt cccgatcacc tcgacgaggt ctccgacgac gacgtcgaca
     2221 ccgaagctgg cgtcgaagac gaagatcccc tcggaggtca gggggttggc gtcggcgtcc
     2281 acgtcttcct cctggagatg gaagccacga agagcagcat tgccctggaa gtcgccgacc
     2341 acaatcccct cgatgatgac ttgggtgccg tcgagtgggc tcgacgctcc cgctccctgg
     2401 atctcgtgga tcttgtacgg caccggtgcc gggggagcga cgaagcatgt gttctcggct
     2461 ccgatcgtcg gggcgaactg agcgaaactc tcgctgttcc gcgccccgcc gctcccattc
     2521 gggcatctct ggctggattg ggctgccccg tcacccccgc cgtcctcgtc gacctggggt
     2581 tggcccgggt tgagcagggc gagcaactcg ggatcgtcgg gatccgaggt cccgtagacg
     2641 acggcatcga tcagaccggt ggtggtgatc acggagttgc ccgggaacga ggt
//
LOCUS       DATITW010000606         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_550798, whole genome shotgun sequence.
ACCESSION   DATITW010000606 DATITW010000000
VERSION     DATITW010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550798"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VLS86_08485"
     CDS             <1..335
                     /locus_tag="VLS86_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSO50571.1"
                     /translation="IVNIASRAYLGNFGQFNYSMSKGAVVGLTRALALSLAPAIRVNA
                     VAPGLISSEMSMGIPEDVRDKMVSAIPLERMGTPDEVANLVWFLLTPLSAYLTGEVIV
                     IGGGRSLS"
     gene            complement(417..863)
                     /locus_tag="VLS86_08490"
     CDS             complement(417..863)
                     /locus_tag="VLS86_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006141807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HSO50572.1"
                     /translation="MEVVARLSALQGEEIGVTDWMEITQERVDAFAEATGDHQWIHVD
                     PVRAAAGPVGGTIAHGFLTLALTVPLAGQVELDVGGPKMAVNYGLEKVRFPAPAPVGG
                     RIRARVTLLAVSEVPGGIQVNRQVTMEVEGGEKPAMVAETVSRYYY"
     gene            909..1214
                     /locus_tag="VLS86_08495"
     CDS             909..1214
                     /locus_tag="VLS86_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HSO50573.1"
                     /translation="MAKETKLMVWIDQDLCTGDGICEEITPDVFVGRDDGLWVVKEEA
                     KYFDRTVVFDGEQAPDGPRGMARIPDALVDDALESAEECPGECIMIEPYNAEALHDG"
     gene            1373..1741
                     /locus_tag="VLS86_08500"
     CDS             1373..1741
                     /locus_tag="VLS86_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50574.1"
                     /translation="MFRKFAVASLFAVLFALPAGAADCQVGSIDWSGGSPRWETGDDG
                     TAGLRTAVVGRFLWVQVGDADPISVELFGDESSATVCLDGTSTFTHAEAVVAEPETVI
                     CDCLPDEYARFIELASFGPR"
     gene            1798..>2469
                     /locus_tag="VLS86_08505"
     CDS             1798..>2469
                     /locus_tag="VLS86_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017622631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HSO50575.1"
                     /translation="MQVLTTRVDPQSEEYRANRAAMEAKLAEFESLMEQARQGGGEMY
                     VARHRERGKLLARERIELLLDRDAPFLELSTLAAWGTDKNLGANVVTGIGVVEGAECA
                     ILAHDPTSQAGSMNRYSVSKTLRIMDIARENRLPLINLVESGGADLPDQAEVFIVGGK
                     WFRNLTRLSKQGIPTLALVFGNSTAGGAYFPAMCDYSVMVKDRAKVFLGGPPLVKMAT
                     GEESTD"
BASE COUNT          438 a          803 c          777 g          451 t
ORIGIN      
        1 ccatcgtcaa catcgcctct cgtgcctatc tggggaactt cggccagttc aactactcga
       61 tgtccaaggg tgccgtggtt gggttgaccc gcgccctggc cctgtcgctg gcgccggcga
      121 ttcgggtcaa tgcggtggcg ccggggctga tctcctcgga gatgagcatg ggcatcccgg
      181 aggacgtgag agacaagatg gtgtctgcga tccctctgga gagaatgggc acccctgacg
      241 aggtggccaa cctggtctgg ttcctgctca cccccctgtc cgcctacctg accggagagg
      301 tgatcgtgat cggcggcggg aggagcctgt cctagggacc ccctcgcctg cggtgccccc
      361 tcccggcttc gccggctctc cccccaacgc tcacttcgtt cgctgggggg agaaactcag
      421 tagtagtagc ggctcacagt ctcggcgacc atcgccggtt tctcgccacc ctccacctcc
      481 atcgtgacct gccgattgac ctgaatccca cccgggacct cggaaaccgc gagcagggtc
      541 acccgggccc gaatccggcc cccaaccggt gccggagccg ggaacctcac cttctccaac
      601 ccgtagttga ccgccatctt cggcccgccg acatcgagct ctacctgtcc ggctaacgga
      661 accgtgaggg ccaatgtgag gaacccgtgg gcgatcgtgc ctccaaccgg cccggcggcg
      721 gctcgcaccg gatcgacgtg gatccactga tggtctcccg tggcctcggc aaaggcatcg
      781 actcgctcct gggtgatctc catccagtcg gtgaccccga tctcctcacc ctgcagcgcg
      841 ctgagcctgg cgacgacttc catattgtgg attctgacac aagcctcgga atcgcctagt
      901 ttggtgacat ggccaaagag accaagctca tggtctggat cgaccaggac ctgtgcaccg
      961 gtgacggaat atgcgaagag atcacccctg acgtgttcgt cggtcgcgac gacggtttgt
     1021 gggtggtgaa ggaagaagcc aagtacttcg accggaccgt ggtcttcgac ggagaacagg
     1081 cgccggacgg cccacggggc atggcccgga tccccgacgc cctcgtcgat gacgcgcttg
     1141 agtcggccga ggagtgcccg ggcgagtgca tcatgatcga gccctacaac gctgaggcgc
     1201 tccacgacgg ctgacgagca tctctcagga cgctccgcaa tgtctcaagg ctgaaccgct
     1261 atctgccgac gcgtacatat cgaaccgcgg acgatcctgt ctccgccctt cccggcatca
     1321 ataacgatcc gcctccccgg cacagaagac aggagcaccc aggaggtgcc ccatgttcag
     1381 gaagtttgcg gttgcttcat tattcgccgt gctgttcgcg ctgcccgcgg gggcagcaga
     1441 ctgtcaagtg gggtcgatcg actggtccgg gggatctccc cgatgggaaa cgggagacga
     1501 cggaacggct ggtctccgta cggcggtggt tggacgattc ctctgggtcc aagtaggcga
     1561 tgccgatccg atcagtgtcg agctcttcgg cgacgagtcg tcggcgacgg tctgtctgga
     1621 cgggacgtcc accttcacac atgcagaggc tgtggtcgcc gagccagaga cagtgatttg
     1681 tgattgtctc cccgatgagt acgcccgctt catcgaactc gcctctttcg ggcctcgcta
     1741 gacgacggac ggggttcaga cgtcgggcct gggtgacaac tacgctcggc tgaccagatg
     1801 caggtactga ccaccagggt cgacccccaa tccgaggagt accgggccaa ccgcgccgcc
     1861 atggaggcca agttggccga attcgagtct ctgatggagc aggctcgaca gggcggtggc
     1921 gagatgtatg tggcccgtca tcgggaacgc gggaagctcc tggccaggga gcgaatcgag
     1981 ctcctcctcg atcgcgacgc accctttctc gagctgtcga cgctggccgc atggggaacc
     2041 gataagaacc tgggcgccaa cgtcgtcacc gggatcgggg ttgtcgaagg agcggaatgc
     2101 gccatcctcg cccacgaccc gaccagccag gccgggtcca tgaatcgcta ttcggtgtcg
     2161 aagacgctgc gcatcatgga catcgcccgc gagaaccgtc tccccctgat caatctcgtc
     2221 gagtccggtg gggcggacct ccccgatcag gccgaggtgt tcatcgtcgg cggtaagtgg
     2281 ttccgcaacc tcacccggct ctccaagcag ggcatcccca ccctggccct ggtcttcggg
     2341 aattcgactg ccggaggcgc ctatttcccc gccatgtgcg actactcggt gatggtcaag
     2401 gaccgggcca aggtgttcct gggcggcccg ccgctggtga agatggcgac tggcgaggaa
     2461 tcgacggat
//
LOCUS       DATITW010000607         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_510012, whole genome shotgun sequence.
ACCESSION   DATITW010000607 DATITW010000000
VERSION     DATITW010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510012"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VLS86_08510"
     CDS             complement(<1..545)
                     /locus_tag="VLS86_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HSO50576.1"
                     /translation="MSGFLFPRTPSGRSSLIPSPPWHYSGEMLTVEYRTDPARVAELL
                     PDGIELAPEDPGAVALIWADWQSCGDSVEELLDPVRSQYKECFVVVRCSFEGQTWSRC
                     VYIWVDKDFALVRGHHQGYPKKLGSIWMTKPVTVGRAGPRLEPGARFGATLAAADRRL
                     ARAMITLEQPAEKPGFVNSHPT"
     gene            635..2014
                     /locus_tag="VLS86_08515"
     CDS             635..2014
                     /locus_tag="VLS86_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSO50577.1"
                     /translation="MSGNLEPGHQERAELSQTVLDFIQDWFDEAGTLPAVGIELDEEL
                     AARLMVPPSEEGRALETILSDVEQAGRSGFYHPSGGHLSYIPNGGLYTAALAEFLAAG
                     LNRYTGVSAAAPGMAAIEHSMVRWMTSLFEMGESASGLLLSGGSMANFTAVVAARTAR
                     LGDDFGRGVIYVTRHTHHSVEKAARLAGFRQDQIVEIAVDADLKMSPDALRSAITADR
                     RSGKDPFLVVGSAGTTDTGTVDPLQAVADLAAAEGLWFHVDGAYGGFFQLTERGRLTL
                     AGLAGADSIAIDPHKGLSIPFGVGALLVRDERFLIDANQGRGAYLRAEDNYLGIRDIA
                     ALGPELSRPFRALQMWLPLHLHGVAPFRQALDTTLDLADAAYRRLSGIEGIETPWRPD
                     LSIVAFRFADDETGRRALEAVNRDRAVHLSPTTIDNRFVLRFAILNRRTTQDHIDHAI
                     DIIEKTLIG"
     gene            complement(2004..>2503)
                     /locus_tag="VLS86_08520"
     CDS             complement(2004..>2503)
                     /locus_tag="VLS86_08520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSO50578.1"
                     /translation="QIVVTRRQISLRDEVEAVAAASGLGDRISISVSPASPGLALGDP
                     NRVRQILRNLSETARRYGGGRIEIELLPDGDQVRVEVRDDGSGVPTSVLDHMFEPYQQ
                     FGGVVGLTESLGLGLSVSAQLAELMQGKLVHQRRGGWTVFSLTLPAVNLDQPAPVGGL
                     AVVSR"
BASE COUNT          433 a          837 c          809 g          424 t
ORIGIN      
        1 gtgggatgcg agttcacgaa gccgggcttt tcggccggct gttccaaggt gatcatcgcc
       61 cgggcgaggc ggcggtcggc cgcggccaga gtcgccccga atcgggctcc gggctccagc
      121 ctgggcccgg cccgaccgac agtgaccggc ttggtcatcc agatgctccc cagcttcttc
      181 gggtaaccct gatgatgtcc gcgaaccagg gcaaagtcct tgtcgaccca gatgtacaca
      241 catcggctcc aggtctggcc ctcgaagctg cagcgcacca cgacgaagca ctccttgtac
      301 tgggagcgca cggggtcgag gagctcctcg acgctgtcgc cacagctctg ccagtcggcc
      361 cagatgaggg cgactgcgcc tgggtcctcg ggcgccaatt cgatgccgtc cggcaacaac
      421 tcggcgaccc gggccggatc ggtccggtac tcgacagtca acatctcccc cgagtagtgc
      481 cacggcggcg aggggatgag cgatgaccgc ccgcttgggg ttcggggaaa gagaaagcct
      541 gacatcaggt gggtgacatg gagtagatcc gctgatcgta gatatcacgc cccggtgtgg
      601 gcgaagcccc ggcaccccga taacctctcc atcgatgtca ggaaacctcg agcccggcca
      661 ccaggaacgg gcggaactga gccaaaccgt cctcgacttc atccaggatt ggttcgatga
      721 ggcgggcacc ctgccggcgg tcgggatcga gctcgacgaa gagctggcag cccgtctcat
      781 ggtcccgcca tcggaggagg gccgggcgct cgagaccatc ctgtccgatg tcgagcaagc
      841 cggtcgctcc gggttctatc acccttccgg agggcacctc tcctatatcc ccaacggtgg
      901 tctctacacc gcggcgctcg ccgagttcct cgccgccggg ctgaaccgat acacgggggt
      961 gtcggcggcg gccccgggga tggcggccat cgagcacagc atggtgcggt ggatgacttc
     1021 cctcttcgag atgggtgagt cggcatcggg actcctgctc tcgggtgggt cgatggccaa
     1081 cttcactgca gtcgttgccg cccgcaccgc gcgtctcggg gacgatttcg gcagaggcgt
     1141 catctatgtc acccgccaca cccatcactc ggtggagaag gcagccagac tcgccgggtt
     1201 tcggcaggac cagatcgtcg agatcgcggt cgatgccgac ctgaagatga gccctgacgc
     1261 cctccgatcg gcgattaccg cagaccgccg atcgggcaag gacccgttcc tggtggtcgg
     1321 ctcggcgggc accaccgaca ccgggacggt cgatcctctg caggcggtcg ccgacctggc
     1381 cgcggcggaa gggctgtggt tccacgtcga tggcgcctac ggggggttct ttcagctcac
     1441 agagcggggc cgactcaccc tggccggtct cgccggcgcc gactcgatcg cgatcgaccc
     1501 ccacaagggc ttgtccatcc cgttcggggt gggagccctc ctggtgagag acgagcgctt
     1561 tctgatcgac gccaatcaag gacgtggcgc gtatctcagg gccgaggaca actacctggg
     1621 aatcagggat atcgccgcac tcgggcccga gttgagtcgt ccgtttaggg cgctgcagat
     1681 gtggctccct ttgcacctgc atggcgtcgc cccatttcgc caggcgctgg acacgaccct
     1741 cgacctcgcc gacgccgcct accgtcgcct atccgggata gaggggatag agaccccatg
     1801 gcggcccgac ctctcgatcg tcgcctttcg cttcgcggat gacgagacag gccgtcgggc
     1861 gctggaagcg gtgaatcggg atcgtgcggt gcatctctcg cccaccacca tcgacaaccg
     1921 cttcgtcctc cgcttcgcca tcctcaaccg gaggacgact caggaccaca tcgatcacgc
     1981 catcgacatc atcgagaaga cgcttatcgg ctgacgacgg ccaggccgcc cacgggtgcc
     2041 ggctggtcca ggttgactgc ggggagagtg aggctgaaca ccgtccagcc accgcgccgt
     2101 tgatggacca actttccttg cattagctca gccagctggg cggagacact gagcccgagg
     2161 ccgagcgact ctgtcaagcc gaccacgccg ccgaactgct gatacggctc gaacatgtgg
     2221 tcgagaacgg aggtggggac gcctgatccg tcgtctctga cctcgactcg cacctgatcg
     2281 ccgtcgggca agagctcgat ttcgatgcgc ccgccgccat agcgtcttgc agtctcgctg
     2341 aggtttcgca ggatctggcg aacccggttc gggtcgccga gcgccaatcc cggcgaagcc
     2401 ggcgagacgc tgatcgagat gcggtcaccc agaccggagg ctgcagcgac agcctcgacc
     2461 tcgtctcgca aagagatctg gcggcgggtc accactatct gcc
//
LOCUS       DATITW010000608         1605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_189716, whole genome shotgun sequence.
ACCESSION   DATITW010000608 DATITW010000000
VERSION     DATITW010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189716"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VLS86_08525"
     CDS             complement(<1..297)
                     /locus_tag="VLS86_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSO50579.1"
                     /translation="MLMRSDPFADFDRLSRQVWGTTRFTFMPVDAYRKDDRFWLHLDL
                     PGVDPESIDLTVEKNTLTITANREWQREDDMQVVLNERPTGTFSRQFFLGEGLDP"
     gene            482..>1605
                     /locus_tag="VLS86_08530"
     CDS             482..>1605
                     /locus_tag="VLS86_08530"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HSO50580.1"
                     /translation="MGQPPSDFERFVGDDRRVMPLGVIVAVIAVAIAVGLVWLFPGET
                     PEIDRTSFGFADEVIPATVTAVEPGECSFAPELACTAVTFGFTTSDGVPSTYLQEFPD
                     ETGQPEFAIGDKVYLSVASFEDGTDSYSYYDRDRGALLGVVALVFAITVIALGRIRGL
                     AALIGLALSLVILIGFIVPAIIAGQDAVLVALVGGGAIALVALYLAHGYTELTHVAAI
                     GAFAALALTTGLSWLVVAAAEFSGLVSEEAFYLLALPDIDLSGLLLAGIVLGTIGALD
                     DVTVTQASAVLEVSQANPELEPRDLFASGLRIGRDHIASTVNTLLLAYAGAAMPLLIL
                     FSLSALPLTVVASSEVVAVEIIRTLVGSMGLVAAVPMTTWL"
BASE COUNT          252 a          518 c          519 g          316 t
ORIGIN      
        1 cgggtcgaga ccctcgccga ggaagaactg gcgggagaag gtgccggtcg gtcgctcgtt
       61 gagcacgacc tgcatgtcgt cctcacgctg ccactcgcga ttggcggtga tggtgagggt
      121 gttcttctcc acggtgagat cgatcgactc gggatcgacg ccggggagat cgaggtggag
      181 ccagaagcgg tcgtcctttc gataggcgtc caccggcatg aaagtgaacc gggtggtgcc
      241 ccagacctgc cgggagagcc gatcgaagtc ggcgaacggg tcggaacgca tcagcatctg
      301 tctttcctcc ttcgagtacg ataactgcat cggactatac acactaaatc tgatacgtca
      361 agtagcagat tagatgatgt tgacgtcgtg ggtaccgtca cccaccgacc ggatgcgaat
      421 cacaggcgga accggcgacc accgctccac acctgggcag cgacccccct acgatgccgt
      481 cgtgggtcaa ccgccatccg atttcgagcg attcgtgggc gacgatcgca gggtgatgcc
      541 gttgggcgtg atcgttgcgg tgatcgcagt ggccatcgcg gtcgggttgg tctggctgtt
      601 ccccggggag accccggaga tagaccgcac ttcgttcgga ttcgccgacg aggtgatccc
      661 ggccaccgtc accgcggtcg aacccggaga gtgcagtttt gcccccgagc tcgcctgcac
      721 cgcggtcacc ttcggcttca ccaccagcga cggggtgcca tcgacctatc tccaggagtt
      781 ccccgacgaa accggccagc ccgagttcgc catcggggac aaggtctacc tgtcggtggc
      841 ctccttcgag gacgggaccg atagctacag ctactacgac cgcgaccgcg gggctctgct
      901 cggagtggtg gccctggtct tcgccatcac cgtcatcgcc ctcggccgca tccgggggct
      961 ggcggcgttg atcgggttgg cgttgagcct ggtgatcctg atcgggttca tcgtcccggc
     1021 catcatcgcg ggtcaggacg cagtcctggt ggccctggtc ggtggtgggg ccatagcctt
     1081 ggtcgccctc tatctcgccc atgggtacac cgagctcacc cacgtggccg ccatcggcgc
     1141 attcgccgct ctcgccctca ccaccgggtt gtcctggttg gtggtggcgg ccgcggaatt
     1201 cagtggcctg gtgagcgaag aggcgttcta tctcctcgcc cttcccgata tcgacctctc
     1261 cgggctcctc ctcgccggga tcgtgctcgg gacgatcggc gcactcgacg acgtgacggt
     1321 gacccaggcg tcggcggtcc tggaggtgag ccaggccaac cccgagctcg agccccgaga
     1381 cctgttcgcc tccggtttgc gaatcggtcg cgaccacatc gcctccacgg tcaacaccct
     1441 gttgctggcc tatgccggtg ccgccatgcc gcttctgatc ctgttcagcc tgtcggcgct
     1501 tccattgacc gtcgtggcca gctccgaggt ggttgcggtg gagatcatca gaaccctggt
     1561 tggatcgatg gggttggtcg ccgcggtccc gatgacaacc tggct
//
LOCUS       DATITW010000609         1524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_551053, whole genome shotgun sequence.
ACCESSION   DATITW010000609 DATITW010000000
VERSION     DATITW010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551053"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /gene="msrB"
                     /locus_tag="VLS86_08535"
     CDS             complement(<1..348)
                     /gene="msrB"
                     /locus_tag="VLS86_08535"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HSO50581.1"
                     /translation="MPQTEAEWRDKLTPEQYEVLREAGTERPFTGKYWDAKTPGTYRC
                     AGCAEELFTSETKYESGTGWPSFYQALRPELIAEVQDVSHGMVRTEVRCANCGGHLGH
                     LFDDGPNPTGLRYC"
     gene            complement(389..859)
                     /locus_tag="VLS86_08540"
     CDS             complement(389..859)
                     /locus_tag="VLS86_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005187820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSO50582.1"
                     /translation="MSRLLRSAGLLPYRLGSGLELLVAHPGGPYFARRDNGWWSIVKG
                     IVEVDESDLEAAAREFAEETGWVPPAQPWIHLGDTVLKSRKVVVAYAVETDFDPLTLE
                     PGTFQMGGRRYPEIDRVEWMAPEQARIKLNPAQGVFIDRLQSHLSQTGNNHLQT"
     gene            872..>1524
                     /locus_tag="VLS86_08545"
     CDS             872..>1524
                     /locus_tag="VLS86_08545"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HSO50583.1"
                     /translation="MTVIQATDRLADHVVLPLPAFRLHAGEPLGTGLKRLGIDEIDRA
                     ISGFYDGEEAFREVVHEARKSIKRIRALLRLIRFEVGEQIYRFENVWVRDSARLISEA
                     RDAAVSVIAIEDMRDLYGSLLAEGTFDETRHKLEVRRERVELRAMEDPSVVPRVVANL
                     ERARARYASWPTDPDARSFYGMGIRDDYRAVGPGLEMTHSRGRREMVVAYKTPSPVH"
BASE COUNT          253 a          496 c          495 g          280 t
ORIGIN      
        1 gcagtagcgg agcccggtgg ggttgggccc atcatcgaac agatgtccga gatggccacc
       61 acagttggcg cacctcacct cagtgcggac catcccgtgg gaaacgtcct gaacctcggc
      121 gatcagctcc ggtctcaatg cctggtagaa gctcggccat ccggtcccgg actcgtactt
      181 ggtctcgctg gtgaagagct cctctgcaca gccggcgcag cggtaggtgc ccggggtctt
      241 ggcgtcccag tacttcccgg tgaacggccg ttcggtgccg gcctcacgga gcacctcgta
      301 ctgctcggga gtgagcttgt ctctccactc ggcttcggtt tggggcatct gtgtcactct
      361 gactcctgtt cttcgcctca gcttcaagtc acgtctggag gtgattgttc ccggtctggg
      421 ataaatgaga ctgcaagcgg tcgatgaaca caccctgggc ggggttgagc ttgatccgcg
      481 cctgctcggg ggccatccac tccacgcgat cgatctccgg atagcgccgg ccccccatct
      541 ggaacgtccc cggctccagg gtcagcgggt cgaaatcggt ctccacggca taggcgacca
      601 ccaccttgcg ggacttgagc acggtgtcgc cgagatgaat ccagggctga gccggaggta
      661 cccaacccgt ctcctcggca aactcgcgtg ccgccgcctc caggtcgctc tcgtcgacct
      721 ccacgatccc tttgacaatc gaccaccagc cattgtctct gcgcgcgaag tagggaccgc
      781 cggggtgggc gaccagtagc tccagaccgg agccgaggcg gtaggggaga agacccgcgg
      841 atctcaagag gcgcgacatt acgctgacct ggtgactgtg atccaggcca cggatcgtct
      901 cgcggatcac gtggtgcttc cccttcccgc gttccggctc cacgccggcg agccgctggg
      961 caccgggctg aaacgtctcg ggatcgacga gatcgatcgc gccatctccg gtttctacga
     1021 cggtgaggag gcgttccggg aggtggtcca cgaggcccga aagtcgatca agcggatccg
     1081 ggccctgctc cggctgatca ggttcgaggt cggggagcag atctatcggt tcgagaacgt
     1141 ctgggtgaga gacagcgccc gactcatctc cgaggctcgc gatgccgcgg tgtcggtcat
     1201 tgccatcgag gacatgcgcg acctctatgg ctcgctcctg gccgaaggga cattcgacga
     1261 gacccgtcac aagctcgagg tccgtcgcga acgggtcgag cttcgggcca tggaagaccc
     1321 cagcgtggtg ccccgtgtgg tggccaacct ggagcgagcc cgggcccgat atgcctcgtg
     1381 gccgaccgac cccgacgcca ggtctttcta cgggatgggg atccgcgacg actaccgcgc
     1441 cgtcggcccc ggcctggaga tgacccattc ccgtggccgg cgggagatgg tggtcgcgta
     1501 caagacgccg agcccggttc attt
//
LOCUS       DATITW010000610         1738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_430647, whole genome shotgun sequence.
ACCESSION   DATITW010000610 DATITW010000000
VERSION     DATITW010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430647"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VLS86_08550"
     CDS             <1..828
                     /locus_tag="VLS86_08550"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HSO50584.1"
                     /translation="AVRGPEVSRRPGDILREVGGLAADGVVEITLLGQNVDTYGRDLA
                     LDGKRRPLFAELLRQVGSIEGIRRVRFTSPHPADFKPEVAAAMAETPAVCEQLHLPLQ
                     SGSDRILAAMHRGYNTARYREKLALARRQIPGLSISTDIIVGFPGETEEDFAATLSLM
                     GEERFDQAFMFIFSPRPGTRAAEMADRFVPHDVIQERFDRLVALQNQVSLELNRAMVG
                     QVVEALAEGPSRKDDEVATTRTRGGKVVHVRGRYRPGTFLDVLVTSAAPHHLIGHPV"
     gene            843..1718
                     /gene="miaA"
                     /locus_tag="VLS86_08555"
     CDS             843..1718
                     /gene="miaA"
                     /locus_tag="VLS86_08555"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HSO50585.1"
                     /translation="MLGPTAAGKSEIAVCLAEELDAPILSVDSMQVYRGLDIGTAKPS
                     LSERERVRHYMIDVVEPEHPYTAAEFQREARTLMMSDRHPLLLIVGGSGLHFRSDVDP
                     LNFPPHDRHVRSDMEAVADPVDALLEVDPRAGEFVDLFNPRRVVRALEVWHLTGLTPS
                     ARSVAEEAMAVQEYEPLYEFTAVGLDPGQVIEARISSRVEAMKDAGLLDEVTSLRGRL
                     GPSAAAAVGYRQLMPVVEGRLDEEDAWSQVRAETLALAKRQRTFFRRDPRIQWVPWED
                     AIDDRLTAVRTALEL"
BASE COUNT          308 a          548 c          577 g          305 t
ORIGIN      
        1 gcggtgcgcg gcccggaggt aagtcgccgg ccgggcgaca tcctcagaga agtcggtggc
       61 ctcgcggccg atggagtcgt cgagataact ctcctgggcc agaacgtcga cacttacggc
      121 cgcgacctcg ctctggacgg caagcgccgt cccttattcg ccgagttact tcgccaggtt
      181 gggtcgatcg aagggatccg acgggtccgg ttcaccagcc cccacccggc agacttcaag
      241 ccggaagtgg ctgcggccat ggcagagacg ccggcagtat gcgagcaact ccatctgcca
      301 ttgcagtcgg gatcggatcg gatcctggca gccatgcacc ggggctacaa cacggcccgc
      361 taccgggaaa agctcgccct ggctcgccgc cagattcccg ggctgtcgat ttcgactgac
      421 atcattgtgg gattcccggg agagacggaa gaagacttcg ctgccactct cagcctgatg
      481 ggcgaggagc gtttcgacca ggcgttcatg ttcatcttct ccccacggcc cggaacgagg
      541 gcggccgaga tggccgaccg gttcgtccct cacgacgtga tccaggagcg cttcgaccgg
      601 ttggtggcgc tccagaatca ggtctctctc gagttgaacc gggccatggt cggccaggta
      661 gtcgaggctc tggccgaagg gccatcgaga aaggatgacg aagttgccac aaccaggacc
      721 cggggaggca aggtcgtgca cgttcgaggt cgctaccggc ccgggacgtt cctcgacgtg
      781 ctcgtcacca gcgcagctcc ccaccacctg atcgggcacc cggtctgagc cgactcgtcg
      841 ccattctcgg cccgaccgct gccggcaaga gcgagatagc agtgtgcttg gcagaggaac
      901 tcgatgcgcc catcctttcg gtcgacagca tgcaggtcta ccggggactg gacataggta
      961 ccgccaagcc ttcgctgtcc gaacgcgaac gtgtgcgtca ctacatgatc gacgtggtcg
     1021 aaccggagca tccctacacg gccgcagaat tccagcggga ggcgcggacc ctcatgatga
     1081 gcgatcgcca tcccctgctc ttgatagtcg gaggctcggg gttgcacttt cgatcggacg
     1141 tcgatccgct caactttcct ccccacgacc gtcacgtgcg atcagacatg gaggctgtgg
     1201 ccgatccggt ggacgcgctg ctcgaggtcg atccccgagc gggggagttc gtcgaccttt
     1261 tcaatccccg tcgggtcgta cgagcgctcg aggtctggca tttgaccggt ttgacgccca
     1321 gcgcccgctc cgtggcggaa gaggccatgg cagtgcagga gtacgagccg ctttacgagt
     1381 tcacggccgt gggcctggac cccggtcaag tgatcgaagc ccggatatct tctcgggtcg
     1441 aggcgatgaa ggacgcaggc ctcctggacg aggtgacgag cctgaggggc cgtctcgggc
     1501 ccagcgctgc cgccgcggtc gggtatcggc agttgatgcc cgtcgtggag gggcgcctcg
     1561 acgaggagga tgcctggtcg caggtccggg cggagaccct ggccctcgcc aagcggcagc
     1621 gcacgttctt tcgccgggat cccaggatcc aatgggtacc ctgggaagac gcgatcgacg
     1681 accggttgac ggcggtgcgg acggccctgg agctttgatg catttcacca agatggaa
//
LOCUS       DATITW010000611         1846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_551074, whole genome shotgun sequence.
ACCESSION   DATITW010000611 DATITW010000000
VERSION     DATITW010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551074"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VLS86_08560"
     CDS             complement(<1..719)
                     /locus_tag="VLS86_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HSO50586.1"
                     /translation="MNPPERRRVVVTGLGAVTPLGEDVGSTWEAMLAGRSGIGSISQF
                     DTDGYRTTIAGEVSGWDPEKHFERREVRRIDRFAQFFLVSVRQALEDAGLSFAGDDPA
                     ATRAGVVVGAGFGGMGTFIEEIVTLTERGPDRVSPTGVPRLIPNMAAGLASIEHNLLG
                     PVSCVVTACAASANAIGDAAEIIRRGAADVMVAGGSEASITTFGIATFAQARALSTRN
                     DDPKAASRPFDLGRDGFVMGEG"
     gene            complement(721..966)
                     /gene="acpP"
                     /locus_tag="VLS86_08565"
     CDS             complement(721..966)
                     /gene="acpP"
                     /locus_tag="VLS86_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HSO50587.1"
                     /translation="MERSEVADRLSGVLVSELGLDAAKISEEAHFEEDLDVDSLGVVE
                     LLMALEDEFGVKIPDEEAESIITVGQAIDGVHGKLGA"
     gene            complement(1002..1751)
                     /gene="fabG"
                     /locus_tag="VLS86_08570"
     CDS             complement(1002..1751)
                     /gene="fabG"
                     /locus_tag="VLS86_08570"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HSO50588.1"
                     /translation="MSDEARGRVALVTGGSRGIGRAIAQRLAASGHVVAVNYASNRDA
                     ADEVVRSITEAGGRAVAYGADVSGVEEVDAMFEAVARDLGPVSVLVNNAGITRDGLLL
                     RMSPKDFDDVIATNLRSAYLCTRLALRGMLRARWGRVLSIASVAGITGNAGQANYAAS
                     KAGLIGLSKSIAKEVGSRGITVNVVAPGFVTTDLTEPLGDRVKESAEASIALGRFGVP
                     EEVAAAVDFLASDAASYITGQVLAVDGGIAL"
BASE COUNT          312 a          657 c          574 g          303 t
ORIGIN      
        1 ccctcgccca tgacgaagcc gtcgcgtccc aggtcgaagg gacgagacgc agccttcggg
       61 tcgtcgttcc gcgtggagag ggcacgggcc tgggcgaagg tggcgatccc gaatgtggtg
      121 atcgacgcct ccgacccacc ggcgaccatc acgtcggctg cgccgcgcct gatgatctcg
      181 gcggcgtcgc cgatggcatt ggccgaagcg gcgcaggcag tgaccacgca agagaccggg
      241 ccgagcaggt tgtgctcgat cgaagccaac ccggcggcca tgttggggat gagtcggggg
      301 actccggtcg ggctgacccg atcggggccc ctctcggtga gggtcacgat ctcctcgatg
      361 aatgtgccca tccccccgaa cccggcgccg accaccaccc cggctcgggt ggcggccggg
      421 tcgtccccgg cgaaggagag ccccgcatcc tccagggcct ggcgcaccga caccaggaag
      481 aactgggcaa atcggtcgat ccggcgcacc tcgcgccgct cgaagtgctt ctcggggtcc
      541 catccggaga cctcgccggc gatcgtggtc cgataaccat cggtgtcgaa ttgcgagatc
      601 gagccgatgc ccgatcggcc ggccagcatc gcctcccaag tggaaccgac atcctcaccc
      661 aggggggtga cagccccgag cccggtgacg acgactcgtc gtcgctcggg tgggttcaac
      721 tcaggctccc agcttcccgt gcacgccgtc gatcgcctgt ccgacggtaa tgatcgactc
      781 ggcctcttcg tccgggatct tgacgccgaa ctcgtcttcc agcgccatca acagctcgac
      841 gacaccaagg ctgtcgacat cgaggtcctc ctcgaaatgc gcctcctcgg agatcttcgc
      901 cgcatccagc ccgagctcgg acaccaacac cccgctgagc cggtctgcta cttcacttcg
      961 ttccacgtac ttctcctttt ggttgaacgg ctggtggaaa gttacagggc gatgccgccg
     1021 tccaccgcca atacctggcc ggttatgtac gaggcggcgt cggatgccag gaaatccacc
     1081 gccgcagcca cttcctccgg gacaccaaag cgtcccaggg cgatcgacgc ctcggcgctt
     1141 tccttgaccc ggtcgccaag gggctcggtg aggtccgtcg tcacgaatcc cggtgcgacc
     1201 acattcaccg tgatcccccg tgaccccacc tccttggcga tcgacttgga gagcccgatc
     1261 aaccctgcct tcgaggccgc gtagttggcc tgaccggcat tgcccgtgat cccggccacc
     1321 gaggcgatcg acaacactct cccccaccgg gcgcgcaaca tgccccggag ggcgaggcgg
     1381 gtgcagaggt acgcagacct gaggttggtc gcgatcacgt cgtcgaagtc cttggggctc
     1441 atccggagca atagaccgtc cctggtaatg ccggcgttgt tcaccagcac ggatactggg
     1501 ccgaggtctc gggcgaccgc ctcgaacatc gcgtcgacct cctccacccc tgacacgtcc
     1561 gcaccgtagg cgacggcgcg accgcccgcc tcggtgatcg accggaccac ttcatctgcc
     1621 gcgtcacggt tcgatgcgta gttgacggcg acgacatgcc ccgaggcggc caggcgttgg
     1681 gcgatggccc tgccgatgcc ccgcgagcca cccgtgacca gggcgacacg cccccgggcc
     1741 tcatcgctca cttcagcttt tcgggcaagc cgaagaagtc gaagttgggc tggagcaact
     1801 ccaaggtggt ccgatccgat ggtggcagct cggcatcgct cacctc
//
LOCUS       DATITW010000612         1803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_230344, whole genome shotgun sequence.
ACCESSION   DATITW010000612 DATITW010000000
VERSION     DATITW010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230344"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VLS86_08575"
     CDS             complement(<1..869)
                     /locus_tag="VLS86_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50589.1"
                     /translation="MTSAIGWVSAAYLATAVLGTWLAVRDGLVGRPFGWAIDMTPLSG
                     FVFGLGTALSAPLVLLLALVAANLLLRQGERAEQRAAGLITILGAGFLAGMLAEPIFW
                     EVFEPGVGALKAATLGANVLLPLSMVILGARARTRPQRIGSSGRVLEVRRLTRPGRAG
                     PPPNVERMKRLAVVVAAIGITLTSCSAAPAAPALSAEQQGELTAQAIAHACADTCTRS
                     QTYVRDQLLDINTLAGDEEPMPVEVSEAIRNAYPEVDFVDREEADVVIAAVDAGEAVM
                     VNVADVAQLAPEVV"
     gene            complement(968..>1803)
                     /locus_tag="VLS86_08580"
     CDS             complement(968..>1803)
                     /locus_tag="VLS86_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50590.1"
                     /translation="PRPRHIGASIQIVPDSRYEMVTDEHRRSDRGRNDGGTRRCSDGR
                     IGERTELTEPRTTDSWAAPVDAFHVGDVAEGARKGNVEGRRPTGPLQGFGRLWQKTYE
                     VRIPGPTPETVISTWKANFGEFWYPSNRFYAPAGGITPGEVAVIGGGKGPTKITTGVR
                     VIYADERSWSYMTPEGHPWAAIITFSAHEDDDGVTVARIHLLVRANDPLYEASFKIYT
                     SRLEDKIWTYTLTRVAGHFGVTEPTIDTTVVLVDKKRQWNQMSNLWKNSAIRTLLRRD
                     R"
BASE COUNT          308 a          599 c          566 g          330 t
ORIGIN      
        1 acaacctcgg gtgcgagctg ggcaacatcg gccacgttga ccatgaccgc ctcgccggcg
       61 tccacggccg cgatcaccac gtcggcctcc tcacggtcca cgaagtcaac ttccggatat
      121 gcgttgcgga tggcttccga gacctcgacc ggcataggtt cttcatcgcc ggcgagagtg
      181 ttgatgtcaa gaagctgatc tcgcacatac gtctggctcc tggtgcaggt gtcggcgcag
      241 gcatgggcga tggcctgcgc ggtgagctct ccctgctgct cagcgctcag cgccggggcg
      301 gcgggcgctg cggaacacga ggtgagagtg atgccgatcg cagcgacaac aacggcgagc
      361 cgcttcatcc gttcgacgtt aggtggcggg ccggctcgtc ctggccgagt caatcgcctc
      421 acctcgagga cccttccgga cgagccgatg cgctgagggc gcgtccgggc ccgggcaccc
      481 aggatcacca tcgacagcgg cagcaagacg ttggcgccca acgtcgccgc cttgagcgcg
      541 ccgactccag gctcgaacac ctcccagaag atcggctcgg cgagcatccc ggcgagaaat
      601 ccggctccga ggattgtgat caggcctgct gcgcgttgct cggccctctc accctgtctc
      661 aggagcaggt ttgctgccac gagggccagc agcaacacga gcggagccga aagagctgtc
      721 cccagcccaa agacgaaccc agacaatggc gtcatgtcga ttgcccagcc aaagggcctg
      781 ccgaccaggc catcccgcac tgcgagccag gttcccaaga ccgcagtggc cagataagcg
      841 gctgacaccc agccgatagc tgacgtcaac ccggcggttg ctattcgcgc tgcttcgcgt
      901 ctgccgccca tttgggctgg tcggtccgtg ccggtcaccg aactgagtcg ccggaggtcg
      961 agcgacgcta gcgatcccga cggagcagcg tccggattgc cgagttcttc cagagattgg
     1021 acatctgatt ccattgccgc ttcttgtcta cgagcacgac agtggtgtcg atggtcggct
     1081 cggtgacgcc gaagtgcccc gcgacccggg tcagcgtgta ggtccagatc ttgtcctcga
     1141 gtctcgaggt gtagatcttg aagctcgcct cgtagagggg atcgttggcc cggaccagga
     1201 ggtggatcct ggccacggtc accccgtcgt cgtcctcatg ggcgctgaag gtgatgatcg
     1261 cggcccaggg atggccctca ggggtcatat atgaccagga gcgctcgtcg gcatagatca
     1321 cacgcacgcc ggtggtgatc ttggtcgggc ccttgccccc tccgatcacc gccacttccc
     1381 cgggtgtaat ccctccggcc ggggcataga acctgttcga cgggtaccag aactcgccga
     1441 agttcgcctt ccaggtggag atcaccgttt cgggggtggg gcccggtatc cgaacctcat
     1501 aggtcttctg ccagagccgt ccgaagccct ggagcggccc cgtgggacgc cggccctcga
     1561 cattgccctt gcgtgcgcct tcggcgacgt ctccgacatg gaaagcgtcc accggggctg
     1621 cccaactgtc tgtggtcctc ggctcggtca actcggtcct ttctccgatg cgcccatcag
     1681 agcagcgacg cgttccgcca tcgttacggc cccgatccga tcgtcgatgc tcatcggtca
     1741 ccatctcgta acggctatcg ggcacaatct ggatcgatgc cccaatatga cgtggtcgtg
     1801 gtc
//
LOCUS       DATITW010000613         1544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_270142, whole genome shotgun sequence.
ACCESSION   DATITW010000613 DATITW010000000
VERSION     DATITW010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270142"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="VLS86_08585"
     CDS             complement(<1..1523)
                     /locus_tag="VLS86_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50591.1"
                     /translation="MQELKLRPPIPRAGQVTRSRVLAMLRSASDIPVISIVAPAGYGK
                     TTTMGQFQAEHPGPTAWATLDASDSDPAVLIRSLTGALSQGGMVDEDLVANLSLQSNQ
                     VLTTGVSTLAAALLPVEQGVLLVDQVDHLLTQSARDVIGAIMTRVAGPIQVILASRSA
                     EGLPIPLLRSQGAVIELSPSDLAMEPAEAAGIFEAAGADIDDAGLESVIERTEGWPAG
                     VYLTAVALKAGAPTLDEAGVGGDDLYFADYLRQELLSQVADSTMSFLIRTSILNRLTG
                     PLCDFILGSGSSSSMLEELETSNLLVVPIDRTRTWYRYHSLLRDYLRSELVRRHPDEV
                     AQLHSRAATWFEDQGLPDEALDLVQSAGEGARFAEMVARSARRVFAEGRVETISGWFD
                     WLEETKTIVEFPEIAGLGAFSRALDGDSAGSERMSVFAFTDSMGNQRDDAELGPFALL
                     LRSHQSARGVDQALTDARAARAGFNSHAEWIHVALGAESMALIARLGIGEAESTWTDA
                     "
BASE COUNT          269 a          484 c          510 g          281 t
ORIGIN      
        1 gcgtcggtcc aagtcgactc ggcctcgccg attcccagcc tggcgatcag agccattgac
       61 tcggccccca atgccacgtg gatccactcg gcgtggctgt tgaaaccggc acgggcggcc
      121 ctggcatctg tgagcgcctg atcgacacca cgagctgact ggtgggaccg cagtagtaga
      181 gcgaaaggcc cgagctctgc gtcgtccctt tggtttccca tgctgtcggt gaaggcgaac
      241 accgacatcc gctcgctccc ggcggagtct ccgtcgagag cgcgtgagaa cgcaccgagg
      301 cctgcgatct cggggaactc gacgattgtc ttggtctctt ccaaccagtc gaaccaacct
      361 gagatggtct cgacccgtcc ttcggcaaag acacggcgcg ccgaccgagc gaccatctcc
      421 gcaaatcgcg ccccctcgcc tgcagattgt acaaggtcga gtgcctcgtc ggggagcccc
      481 tggtcctcga accaagtagc tgcccgcgag tggagctggg cgacttcgtc tggatgtctg
      541 cggacgagct cggaacggag gtagtcgcgg agaaggctgt gatagcggta ccaggtgcgg
      601 gttcggtcga tggggacgac gaggaggttg gaggtctcca gttcttccag catggacgat
      661 gagctcccac tccccaggat gaagtcgcag agcggtcctg tcagtctgtt gaggatcgag
      721 gttctgatca ggaatgacat cgtcgagtcg gcgacctgac tcagcagctc ctgacggaga
      781 tagtccgcga agtagaggtc gtcgccgccc acccccgcct cgtcgagcgt gggggctccg
      841 gctttgaggg cgactgcggt cagatagacg ccggccggcc acccctcggt tcgctcgatc
      901 acactctcca gcccggcgtc gtcgatgtcg gcgcctgctg cttcgaatat cccagcagcc
      961 tcggccggtt ccatggccag gtccgaaggc gatagttcga tgacggctcc ttgggatcgc
     1021 agcagtggaa tagggaggcc ctccgccgaa cgagaggcga ggatcacctg gatcggcccg
     1081 gcgacacgcg tcatgatcgc tccgatcaca tcccgtgcgc tttgagtcag cagatggtcc
     1141 acctggtcga ccagcagcac accctgctcc acggggagta gagctgccgc cagggtgctg
     1201 acaccggtgg tcagcacctg gttggactgc agggacaggt tcgctaccag atcctcatcg
     1261 accattcccc cttggctcaa ggcgccagtg aggctcctga tgagcacagc cgggtcgctg
     1321 tcgctggcgt ccagggtggc ccaggcggtg gggcctgggt gctcggcttg gaactgcccc
     1381 atcgtggtcg tctttccgta tcccgccggc gcaactatcg agatcaccgg gatgtcggat
     1441 gcggaacgga gcatggcgag gaccctggag cgcgtgacct ggccggcccg agggatggga
     1501 ggacggagtt tcagttcctg catgggagga ttcctaacgg gccc
//
LOCUS       DATITW010000614         1688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_189902, whole genome shotgun sequence.
ACCESSION   DATITW010000614 DATITW010000000
VERSION     DATITW010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189902"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VLS86_08590"
     CDS             <1..366
                     /locus_tag="VLS86_08590"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041065.1"
                     /note="cleaves the ring of
                     2,3-dihydro-2,3-dihydroxybenzoyl-CoA forming
                     6-hydroxy-3-hexenoyl-CoA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA-dihydrodiol lyase"
                     /protein_id="HSO50592.1"
                     /translation="LETRFWGDPEGLSTARGVIGEKLDAEAALAAGLVTDAMDDLDWD
                     DELRITIEERSAFSPDALSGMEANLRFPGPETMETKIFGRLTAWQNWVFQRPNASGEA
                     GALRRYGTGIRPEYDPRRV"
     gene            366..>1688
                     /gene="boxB"
                     /locus_tag="VLS86_08595"
     CDS             366..>1688
                     /gene="boxB"
                     /locus_tag="VLS86_08595"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HSO50593.1"
                     /translation="MGWQDVDYDTLIPNNVGLASDPKLQRALERWHPGYIDWWMSMGP
                     EGFQMADVYLRTAISVEKEGWAKFGYVKMPEYRWGILLAPDEEGRTIPCGEHVGEPAW
                     QEVPGEYRSMLRRLIVIQGDTEPASVEQQRHLGSTAPSLYDLRNLFQVNVEEGRHLWA
                     MVYLLQKYFGRDGRAEADELLRRQSGNAERPRMLGAFNEETPDWLSFFMFTYFTDRDG
                     KMQLEALAQSGFDPLSRTCRFMLAEEAHHMFVGYTGVMRIVEATAKVMAQAGVTDPYD
                     VESVHSLGVIDLPAIQRKVNLHYSLTLDLFGSEESTNAANFFNSGIKGRYHENRLDDD
                     HQLRDATYPVMKLVDGELQLVDVPALNGLNARLRDDFIDDCMGGIKRWNKEIESHGIE
                     FEITLPHVAFNRAIGEFGDLSVTPNGLVVTEKEFETARHEWLPTSDDRE"
BASE COUNT          336 a          553 c          516 g          283 t
ORIGIN      
        1 ctggagactc gattctgggg tgaccccgag ggtctctcca ctgcccgggg ggtgatcggt
       61 gagaaactcg acgcagaagc cgccctcgcc gcgggtctgg tcaccgacgc catggacgac
      121 ctcgactggg atgacgagct ccggatcaca atcgaggaac gatccgcctt ctcgcccgac
      181 gctctgtccg gcatggaagc aaatctccgc ttcccaggcc ccgagaccat ggagaccaag
      241 atcttcggac ggctcaccgc ttggcagaac tgggtgtttc agcggccgaa cgcttcgggc
      301 gaagccggcg ccctgaggag atacggaacc ggcattcgac ccgaatacga cccgaggaga
      361 gtgtgatggg ctggcaagac gtcgattacg acaccctgat ccccaacaac gtcggtctgg
      421 catctgaccc caaactgcag cgggccctgg aacggtggca ccccggctac atcgactggt
      481 ggatgtcgat gggcccggaa ggcttccaga tggccgatgt ctatctccgc accgccatct
      541 cggtggagaa ggagggctgg gccaagttcg ggtatgtgaa gatgccggag taccgctggg
      601 ggatcctcct ggcgcccgac gaggaaggtc gcaccatccc ctgcggcgag catgtcggtg
      661 agccggcatg gcaggaggtg cccggcgagt accggtccat gctccgtcgt ctcatcgtca
      721 tccagggcga caccgagccc gcctccgtcg agcagcagcg tcacctcggc agcacagccc
      781 cgtcgttata cgatttgcgc aatctcttcc aggtgaacgt cgaagaggga cgtcatctat
      841 gggcgatggt ctatctgctc cagaagtact tcggacgtga cgggcgggcc gaggccgatg
      901 aactgctccg ccgccagtcg ggcaacgccg agcggccccg gatgctcgga gcgttcaacg
      961 aggagacccc ggactggttg tcgttcttca tgttcaccta cttcaccgac cgcgacggca
     1021 agatgcagct cgaagccctg gcccaatccg gattcgaccc gctctcccgc acctgccggt
     1081 tcatgctcgc cgaagaggcg caccacatgt tcgtcgggta caccggggtc atgcggatcg
     1141 tcgaggccac cgccaaggtc atggcccaag ccggagtgac cgacccatat gacgtcgagt
     1201 cggtccactc cctcggtgtg atagacctgc ccgccatcca acgcaaggtc aacctccact
     1261 actcgctcac cctcgacctg ttcggatcgg aggagtcgac caacgccgcc aacttcttca
     1321 actccggcat caagggccgc tatcacgaga accgcctcga cgacgatcac caactgcgcg
     1381 acgccaccta cccggtgatg aaactggtcg acggggaact gcagctggtc gacgtccccg
     1441 cactgaacgg gttgaacgcc cggctccgcg acgacttcat cgacgattgc atgggtggga
     1501 tcaaacggtg gaacaaggag atcgagagcc acggcatcga attcgagatc accctgcccc
     1561 acgtcgcgtt caaccgggcc atcggagaat tcggcgatct ctcggttacc cccaacggtc
     1621 tggtggtcac tgagaaggaa ttcgagactg cccggcacga gtggctgcct accagcgacg
     1681 accgggag
//
LOCUS       DATITW010000615         3562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_30255, whole genome shotgun sequence.
ACCESSION   DATITW010000615 DATITW010000000
VERSION     DATITW010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30255"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VLS86_08600"
     CDS             <1..267
                     /locus_tag="VLS86_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HSO50594.1"
                     /translation="GEALSLADEVVVTDVYGSREVPVPGVTGELVADAARDHGASVHY
                     VPRITDLAPYLADRVASGDLIVTMGAGDVTLVHTELAMRLAARQ"
     gene            264..1169
                     /gene="murB"
                     /locus_tag="VLS86_08605"
     CDS             264..1169
                     /gene="murB"
                     /locus_tag="VLS86_08605"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HSO50595.1"
                     /translation="MSGLAALISAGAVEEEVDLGPFTTYKSGGPARYFMEVRSPEALA
                     DVARSGDLGDLPILVLGRGSNLVVSDSGFPGLVLRLGAAFGELRIEDSVVTAGSAAPL
                     PQVARASVNAGLTGLEFFVGIPGSIGGAVRQNAGCFGSETRDRLITASILDLYGGEER
                     TMTPGQLEMSYRHSNLKPTQVVLEASFQTTLGDMARGLEELRRITRWRRDNQPGGTFN
                     AGSVFKNPPGITAGEIIDSLGLKGMRVGDVAVSTKHANFFVAGPDATSSEIRRLVVAV
                     KDRVFQETGTILETEIQFVGFENDD"
     gene            1159..1932
                     /locus_tag="VLS86_08610"
     CDS             1159..1932
                     /locus_tag="VLS86_08610"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HSO50596.1"
                     /translation="MTIDPRLIDRRREVAEDRARKNVRRLVRFLVVLAILGGLVWLLL
                     SPLLSVEEVITTGIVSSSAHAALVDEEVLAGRPMILIRPSEIVATLQADPWVREATVE
                     LDWPNRVLVRVEERVPVGWVETGDGWSRRAVDGVAVPSSTEPDPSMVWAHFPDLPESE
                     AGESRHVIGALEFAETLPTELRSGAHVAVAGNGEMWAEVSGFRVRLGRPIEMRAKALS
                     LSALLAESPEPGSTLNLIAPTHPAITPPISDEKTPEEEP"
     gene            2028..3083
                     /gene="ftsZ"
                     /locus_tag="VLS86_08615"
     CDS             2028..3083
                     /gene="ftsZ"
                     /locus_tag="VLS86_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HSO50597.1"
                     /translation="MSTSSGGYLAVIKVVGIGGGGVNAVNRMVEAGIRGVEFIALNTD
                     AQALLMSDADVKLDIGREVTRGLGAGANPEIGRAAAEEHKAELEDALKGSDMIFITAG
                     EGGGTGTGGAPVVAEVARSVGALTVGVVTRPFGFEGRRRAVHAEQGIDLLKDSVDTLI
                     VIPNDRLLDVGDPSAPMTETFRLADEVLMSGVAGITDLITTPGLINVDFADVRTIMAD
                     AGSAVMGIGRATGENRAKQAAERAISSPMLETSMEGAKGVLLSVAGPSSMTLHEATEA
                     ARVVSDHADPEADIIFGAIVDDDLGEEMKVTVIAAGFERRRQTNRPAVARPTRPSYSS
                     LDEGDDDSLDIPTFVRG"
     gene            3080..>3562
                     /locus_tag="VLS86_08620"
     CDS             3080..>3562
                     /locus_tag="VLS86_08620"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HSO50598.1"
                     /translation="MKRPPGGNGVAFTACGDGDQRHDLPARSAVSSWLGISRSWATLR
                     QVHGNPVVRATSPGELGEADGLWTDLPDLPLAVFTADCFGVVLISRDAVGVAHAGWRG
                     AAERVVTALRAEMESEGHVPTAAAIGPGIGPCCFEVGPEVAERFPDEVTETTWGALSV
                     D"
BASE COUNT          594 a         1093 c         1274 g          601 t
ORIGIN      
        1 ggtgaggcgc tgagcctcgc cgacgaggtg gtggtcaccg acgtatacgg ctcgagggag
       61 gttccggtgc ccggcgtcac cggggagctg gttgccgacg cggcgcgcga ccacggcgcc
      121 agcgtgcact atgtgccccg catcaccgac ctggctccct atctggcgga tcgggtggcc
      181 agtggcgatc tgatcgtcac catgggagcc ggggatgtga ccctggtcca caccgagctg
      241 gcgatgagac tcgccgcgcg ccaatgagcg ggctcgccgc tctgatctcg gcaggcgccg
      301 tcgaggaaga ggtggatttg ggccctttca ccacctacaa atcgggaggc ccggcccgct
      361 atttcatgga ggtgcgcagc cccgaggcgc tggccgatgt ggcccgctcc ggagatctgg
      421 gcgatctccc gatcctggtc ctgggccgcg ggtccaatct cgtggtgtcg gactccgggt
      481 ttcccggtct ggtgttgcgg ctcggcgccg cattcgggga gttgcgaatc gaagactcag
      541 tggtgacggc agggagcgcc gcgccgcttc cccaggtggc tcgggccagc gtcaatgccg
      601 ggctcaccgg tctcgagttc ttcgtgggca tcccgggatc gataggcggc gcggtccgcc
      661 agaacgcggg ctgcttcggc agcgagaccc gggaccgtct gatcacggcg agcatcctcg
      721 acctgtacgg cggcgaggag cgcacgatga ccccgggcca gctcgagatg tcctatcggc
      781 actcgaacct gaagccgacc caggtggtgc tcgaagccag ctttcagacg acgctgggcg
      841 acatggcgag aggtctcgaa gagctgaggc ggatcacccg gtggcgtcgt gacaaccagc
      901 caggtgggac gttcaatgcg gggagtgtgt tcaagaaccc accggggatc accgccgggg
      961 agatcatcga ctcgctgggg ctgaagggga tgagggtcgg tgacgtcgcg gtgtcgacca
     1021 agcacgccaa cttcttcgtc gccgggcccg acgccacgtc gagtgagatc cgaagactgg
     1081 tcgtggcggt gaaagaccgc gttttccagg agaccgggac cattctggaa acagaaatcc
     1141 aattcgtggg attcgagaat gacgattgac ccgcgtttga tcgaccgcag acgggaagta
     1201 gccgaggatc gggcgcgaaa gaacgtccgt cgcctggtcc gcttcctggt ggtgctcgcc
     1261 atcttgggcg ggctggtctg gctgctgctg tcgccacttc tcagcgtcga agaggtgatc
     1321 accaccggca tcgtctccag ctccgctcat gctgcgctcg tcgacgagga ggtcctggcc
     1381 ggcaggccca tgatcctgat ccggccctcg gagatcgtgg ccaccctgca ggccgacccc
     1441 tgggtccgcg aggcgacggt cgagctggat tggccaaacc gggtgttggt cagggtggag
     1501 gagagggtgc ccgtgggatg ggtcgagacc ggtgacggct ggtcacggcg agcggtggat
     1561 ggtgtcgccg tgccctcctc gaccgagccc gacccctcga tggtgtgggc ccatttcccc
     1621 gatctgcccg agtcggaggc aggggagtcg cgccacgtga tcggtgcgct ggagttcgcc
     1681 gagaccctcc ccaccgagct gagatcgggg gcccatgtgg cggtggccgg caacggggag
     1741 atgtgggccg aggtatcagg gttccgggtg aggctgggga gaccgatcga gatgagggcc
     1801 aaggcactga gtctgtccgc tttgctcgcg gagagccccg aacctggctc cacgttgaac
     1861 ctgatcgccc ccactcatcc cgcaatcacc ccaccgatca gcgacgagaa gacccctgag
     1921 gaggaaccct gaacgtctag ttgaggctta tggattgagg ctttactaaa gcaaagccca
     1981 acggtaaacc tctactttag cttcgtattc aacctggagg atttgttgtg agcacgtcga
     2041 gcggtggcta tctagccgtg ataaaagtgg tcggaatcgg cggcggtggt gtcaacgcgg
     2101 tgaaccggat ggtcgaggcc gggatccgcg gcgtcgagtt catcgccctc aacaccgacg
     2161 cccaggccct gctgatgagc gacgccgacg tcaagctcga catcggtcgc gaggtgaccc
     2221 gtggtctcgg tgccggggcc aaccccgaga tcggccgggc ggccgccgag gagcacaagg
     2281 ccgagctcga ggatgcgctc aaggggtcgg acatgatctt catcaccgcc ggtgaaggtg
     2341 gtggcaccgg taccggaggc gctccggtgg tggccgaggt cgcccgttcc gtgggagcgt
     2401 tgacggttgg ggtggtgacc cgcccgttcg ggttcgaggg aagacggcgc gcggttcatg
     2461 ccgagcaggg catcgacctg ctgaaggact cggtggacac gttgatcgtc atcccaaatg
     2521 accgtcttct cgatgtcggc gacccgagcg cgccgatgac cgagaccttc cggctcgccg
     2581 acgaggtgct gatgagcggc gttgccggga tcaccgacct gatcaccacc ccgggcttga
     2641 tcaacgtcga cttcgccgac gtgcggacga tcatggccga cgccggctct gcggtgatgg
     2701 gaatcgggcg cgccaccggc gagaaccggg ccaagcaggc tgcggagcgg gcgatctcca
     2761 gccccatgct cgaaacctcc atggaggggg caaaaggtgt cctgctctcg gtagccggcc
     2821 cctcctcgat gacgttgcac gaggcgacgg aggcagcccg agttgtatcg gaccatgccg
     2881 atcccgaggc ggacatcatc ttcggggcga tagtcgacga cgatctcggc gaggagatga
     2941 aggtgacggt gatcgccgcc ggattcgagc ggagacggca gaccaaccgg ccggcggtgg
     3001 cgcgtcccac ccggccctcc tactcgagtc tcgacgaagg cgacgacgac agcctcgata
     3061 tcccaacctt cgtcagggga tgaagcgtcc ccccggcggc aacggggtgg cattcaccgc
     3121 ctgtggagac ggcgatcagc gccacgatct tccggctcgc tccgcggtgt cctcgtggtt
     3181 gggcatctct cgttcctggg cgaccctgag acaggttcac gggaacccgg tggtcagggc
     3241 caccagcccg ggggagttgg gcgaggccga cgggttgtgg accgatctcc ccgatctgcc
     3301 gctagcggtc ttcaccgccg actgcttcgg ggtggtgctc atctccaggg atgcggttgg
     3361 agtcgcccac gccgggtggc ggggcgccgc ggaacgggtg gtgactgcgc tcagagccga
     3421 gatggagagc gaaggtcacg tcccaaccgc agccgccatc gggcccggga tcggcccctg
     3481 ttgcttcgaa gtcgggccgg aggtggccga gaggttcccc gatgaggtga ccgagaccac
     3541 ctggggggcg ctgtcggtcg at
//
LOCUS       DATITW010000616         1923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_189968, whole genome shotgun sequence.
ACCESSION   DATITW010000616 DATITW010000000
VERSION     DATITW010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189968"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VLS86_08625"
     CDS             complement(<1..646)
                     /locus_tag="VLS86_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50599.1"
                     /translation="MTRRDRAVVLSLTLALTACSAGAGLQVLSSPEDKIRVERTAQTF
                     LQTLPFKYLGRSYTLDIMGDDRAAAWNVAPGIIYISQDAARYASDEELAQLIAHTLGH
                     DLLAHPGIRTDVSDGRLAAEFTTITVVPGGLLLGGIVEAMTDSTDYTLAQEIEAEKIG
                     LRLWLRSGRSCTTWIGLRAGQKERGRSWHEPIKGVAPPLDDLTAVAERECGQQPS"
     gene            complement(1096..>1923)
                     /locus_tag="VLS86_08630"
     CDS             complement(1096..>1923)
                     /locus_tag="VLS86_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HSO50600.1"
                     /translation="QWPSAATVGAAGSTELVHTMARAMAQELLVVGINMNLAPVLDVL
                     TNPANPIMAGRCFGNDPQVVTRLGMAVFGGLKDMGVLAVGKHFPGHGDTTVDSHLGLP
                     VVPHDQARLWAVELAPFVAAIDAGIQALMTAHLLVPALDPERPATLSRPILTDLLRGR
                     LGFAGLLVSDDLLMRGIADTTPPGEAAVQFLEAGGDCVLICHDEAVQRQAILAVTEAV
                     EMGRLSEARVQLSCDRIAKAKAQYLGRGMPASAETIREVVGCAAHRRVAELLRGGAT"
BASE COUNT          360 a          632 c          606 g          325 t
ORIGIN      
        1 tcgagggctg ctgtccgcat tctctttcgg ccaccgcggt caagtcgtcg agcggcggtg
       61 ccacaccctt aatcggctca tgccagctcc ttccccgctc cttctgcccc gcgcgtagtc
      121 cgatccaggt cgtgcagctc cgaccgctgc gcagccagag ccgcaggccg atcttttccg
      181 cttcgatctc ctgggccagg gtgtaatccg tgctatcggt catcgcttcg acgatgcccc
      241 ccaagagaag acctcccggc acgaccgtga tggtggtgaa ttccgccgcc aagcgaccgt
      301 ccgatacatc cgttcgaatc ccgggatggg ctagaaggtc atgacccaag gtgtgcgcga
      361 taagttgggc cagctcttcg tcggaggcat accgagcggc gtcctgactg atgtagatga
      421 tcccgggcgc cacgttccat gcggcggccc ggtcatcgcc catgatgtca agggtgtacg
      481 atcgcccgag atacttgaat gggagggtct gaagaaacgt ctgggcagtg cgctccacgc
      541 ggattttgtc ctcgggagag gacaggactt gcaggccggc gcccgcgctg caggcagtga
      601 gggccagcgt gagggagaga acgaccgcgc gatctcgacg cgtcacccga tggcccttct
      661 caggtggcgg gacatggcaa tcaagagatc agggtcctca gtcgagtccc tccttcactg
      721 cgaacgcggt gagccgacga cgagacgacc ctcacgcgga ccctccccgc tcggcctatc
      781 caggggcatt atgcacgaag ggatacgccg aagtaaacga tttcttgaaa tgtgccattt
      841 gggtctgatc tccgtggagg accctgaacc ccatgagcat tcgatgtggc gagaatggat
      901 gggatgaact agtgctcctc caaccatttc gccctcagtt gatggatacc ctgccgggcc
      961 aagaagcgag cagagcatgg cgcggccccc gcggaccgac cgaggcgtac actgtcaggt
     1021 acgtcgcagg gagtgtcccg gggtgacgca acgccgctag gcgatgttaa ttggaccggc
     1081 actagaagcc cattcctacg tcgctccgcc ccgcaacagc tccgccaccc gacggtgggc
     1141 ggcgcagccc acgacctcac gtatcgtctc ggcggaggcg ggcatccccc gacccaagta
     1201 ctgggccttt gccttggcga tccgatcgca tgagagctgg acgcgcgcct cggagaggcg
     1261 ccccatttcg accgcctcgg tcacggccag gatcgcctgt cgctgcaccg cctcgtcgtg
     1321 acagatgaga acacaatcac ccccggcttc gagaaactgg acagccgcct cgccaggggg
     1381 ggtcgtgtcg gcaatgccgc gcatcaacag gtcgtcgctg acgagcagac cggcgaaccc
     1441 gagccgccca cgcaagaggt ccgtgaggat cgggcgcgac agggtggccg gccgttcggg
     1501 gtccagggcc ggaaccagga gatgcgcggt catgagcgcc tggatccccg cgtcgatggc
     1561 cgctacaaac ggggcaagtt ccaccgccca gagccgagcc tgatcgtgcg gcacgaccgg
     1621 cagccccagg tgagagtcca cggtcgtgtc accgtggccg ggaaagtgct tgcccaccgc
     1681 gagcaccccc atatctttga gcccaccgaa gacggccatg ccgagtcgag tcaccacctg
     1741 cgggtcattg ccaaagcaac ggccggccat gatcgggttc gccggattgg tcaggacatc
     1801 caggaccggc gccaggttca tattgatccc tacaaccagg agttcctggg ccatcgcgcg
     1861 agccatagtg tggaccagtt cagtcgaacc ggctgccccg accgttgccg cggatggcca
     1921 ctg
//
LOCUS       DATITW010000617         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_470778, whole genome shotgun sequence.
ACCESSION   DATITW010000617 DATITW010000000
VERSION     DATITW010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470778"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..>1530)
                     /locus_tag="VLS86_08635"
     CDS             complement(144..>1530)
                     /locus_tag="VLS86_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018090914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HSO50601.1"
                     /translation="DGPSTEAGELALGRNLLVAFMPWQGHNFEDAIIISERLVKDDVL
                     TSIHIEEHEIDARDTKLGAEEITRDIPNVPEEVLANLDEEGIIRVGAEVGPGDYLVGK
                     VTPKGETELTPEERLLRAIFGEKAREVRDTSLKVPHGETGKVIAVKVFKRAEGYDLPP
                     GVNELVRIYVAQQRKISEGDKLAGRHGNKGVISKVLAEEEMPFMADGTPVDIILSPLG
                     VPSRMNLGQVLETHLGWAAAQGWEPFKGESPAHIGADAWTKVATPVFDGAREDEIMQV
                     ISSARTNRDDERLVADDGKAVLYDGRSGEPFDTPITVGFIYMLKLVHLVDEKIHARST
                     GPYSMITQQPLGGKAQFGGQRFGEMEVCALEAYGAAYALQELLTIKSDDVQGRVKVYE
                     SIVKGDNIPEPGIPESFRVLVKEMQSLCLNVEMLAAGEEVELRELEEESYRSVETLGI
                     DLSRPERPDSE"
BASE COUNT          243 a          496 c          481 g          310 t
ORIGIN      
        1 ggactttggc cgggagtctc ctcccaccag ccgtcttccc aatccgggga caacgtccat
       61 catctgcaaa tctcgctcta tccgtcggcc aaacagacgc cggtccgccg gagcttccgc
      121 cgcgtattgc gtatctgccg atctcattcc gagtcggggc gctcgggtct gctcaaatcg
      181 atgccgaggg tctcgaccga ccggtaactc tcttcctcga gctccctgag ctcgacctct
      241 tcaccggcgg ccagcatctc gacgttgaga cagaggctct gcatctcctt gaccaggacc
      301 cggaacgact ccgggatgcc tggctcgggg atgttgtctc ccttgacgat cgactcgtag
      361 accttgaccc gtccctgcac gtcgtcggac ttgatcgtga gcagctcttg cagggcgtag
      421 gcggcgccat aggcctcgag ggcacagact tccatctcgc cgaaccgctg gccaccgaac
      481 tgggccttcc caccgagggg ctgctgggtg atcatgctgt aggggccggt cgaccgggcg
      541 tggatcttct cgtcgaccag gtgaaccagc ttcagcatgt agatgaagcc caccgtgatc
      601 ggggtgtcga agggctcgcc actgcggccg tcgtagagca ccgccttgcc gtcgtcggca
      661 accagtcgct catcgtcacg attggtgcga gcgctgctga tcacttgcat gatctcgtcc
      721 tcgcgggcac cgtcgaacac aggggtcgcg accttggtcc atgcgtcggc gccgatgtgg
      781 gccgggctct ctcccttgaa cggctcccaa ccctgggcgg cggcccaacc gagatgggtc
      841 tcgaggacct gaccgaggtt catgcgggac gggaccccga gtggggagag aatgatgtcg
      901 accggggtgc catcggccat gaacggcatc tcttcctcgg cgagaacttt ggagatgacg
      961 cccttgttgc cgtgacggcc cgccagcttg tcgccttcgg agatcttgcg ctgttgggcg
     1021 acgtagatcc gaaccagctc gttgacgccg gggggaaggt catagccctc ggccctcttg
     1081 aagaccttga ccgcgatgac cttgccggtc tcaccgtggg ggaccttgag tgaggtgtca
     1141 cgcacttcac gcgccttctc gccgaagatc gcacgaagca aacgctcctc gggcgtgagc
     1201 tcggtctcac ccttgggggt caccttgccg accaggtagt caccgggacc gacttcggct
     1261 ccgacccgga tgatcccctc ctcgtcgagg ttggccagga cttcctcggg gacgttgggg
     1321 atgtcccggg tgatctcctc ggcaccgagc ttggtatcgc gggcgtcgat ctcatgctcc
     1381 tcgatgtgga tcgaggtgag gacgtcgtct ttgaccaggc gctcggagat gatgatggcg
     1441 tcttcgaagt tgtggccctg ccatggcatg aatgcgacca acaggttccg accgagcgcc
     1501 agttccccgg cctcggtcga cgggccgtcg
//
LOCUS       DATITW010000618         1571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_150246, whole genome shotgun sequence.
ACCESSION   DATITW010000618 DATITW010000000
VERSION     DATITW010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150246"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..1169)
                     /locus_tag="VLS86_08640"
     CDS             complement(306..1169)
                     /locus_tag="VLS86_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HSO50602.1"
                     /translation="MVSPERVGVIGGGLMGSGIAEVFARSGSSVIVHEIDAGTAEAAR
                     SRIESSLAKAVERGKLDAASRDQTLANIVVSIDLGDQADRQLVIEAATENEGLKKQVF
                     ADLDAVVADREAILASNTSSIPITRLASVTSRPDHVIGLHFFNPVPVMALVELVTTVM
                     TAPEVATRAKAHAEGLGKTVIESRDRAGFIVNMLLVPYLLGAIKMLEAGFATREDIDA
                     GMRLGANHPMGPLELADFVGLDTMKLIADVLFDEFKDPMYASPPLLTRMVEAGLLGRK
                     SGRGFYDYGGT"
     gene            1251..>1571
                     /locus_tag="VLS86_08645"
     CDS             1251..>1571
                     /locus_tag="VLS86_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSO50603.1"
                     /translation="MSYDHQKIEAKWQERWADERLFNVTEDVSKPKFYNLCMYPYPSG
                     ALHQGHVRNYTFGDLLTRYKTMRGFNVLSPMGWDSFGLPAENAAIETGADPRENTEAQ
                     IATMT"
BASE COUNT          295 a          504 c          487 g          285 t
ORIGIN      
        1 gcgatagatg atcgacgcac aatagatggc gaacagccgg gcccaggtct cgtcccaagt
       61 tccaagcagg tgtcggtctg tctcgagcat cgagcaaagg ggcgcgaggg tgtctgacgc
      121 cgtggatctc tgacttccga gcaccaaggg ctgataggcc aacatcttcg actcccagtt
      181 ccttctcgag ctcccacgac ttaccttgtg gataattccg gaatagttgt gagttgggca
      241 tgggccgggt ggcccatttc ccgggtgtca tccgagctag tcccagcgac ctatgccgga
      301 gccggtcacg ttccgccgta gtcgtagaag ccgcgtcccg atttccgccc gaggaggccg
      361 gcctcgacca ttcgggtgag gagcggtggg gaggcgtaca tcgggtcctt gaactcgtcg
      421 aagagaacgt ccgcgatcag cttcatggtg tcgaggccaa cgaagtcggc caactccaga
      481 ggccccatcg ggtggttcgc cccgagccgc atcccggcgt cgatgtcctc tctggtggcg
      541 aatccggcct cgagcatctt gatggcaccg aggagatagg ggaccaggag catgttgacg
      601 atgaagccgg cgcggtccct cgactcgatc acggtttttc cgagcccttc ggcgtgggcc
      661 ttggcccgtg ttgccacctc gggtgccgtc atcaccgtgg tgaccaactc gaccagggcc
      721 atgaccggga ccgggttgaa gaagtggagc ccgatcacgt ggtcgggccg gctggtcacc
      781 gaggcgagac gggtgatcgg gatcgaggag gtgttggagg cgaggatggc ctcccgatcg
      841 gcgacgacag cgtcgagatc ggcgaagacc tgtttcttga gcccctcgtt ctcggtggct
      901 gcctcgatca ccagctggcg gtccgcctgg tcgccgagat cgatcgatac cacgatgttg
      961 gccagcgtct ggtcccgtga cgcggcatcc agcttgcccc gttccacggc tttggccagc
     1021 gaggactcga ttcgggagcg agcggcctcg gcggtcccgg catcgatctc gtggacgatc
     1081 accgagctcc ccgacctggc gaagacctcg gcgattcccg accccatcaa cccgccaccg
     1141 atcacaccga cacgttctgg gctcaccagg cgcagcctcc gtaattagga gcaacaggat
     1201 cgtatatgac ccgtctccgt cccaattggg tggaggtact ctcaccagcg atgtcctacg
     1261 accatcagaa gatcgaagcc aaatggcagg agcgctgggc cgacgaacgc ctgttcaacg
     1321 tgaccgaaga cgtctccaaa cccaagttct acaacctctg catgtacccc tatccatcgg
     1381 gggcgctcca ccagggtcat gtccgcaact acaccttcgg ggacctcctc accaggtaca
     1441 agacaatgcg tggcttcaac gtgctctcgc ccatgggctg ggacagcttc ggcctccccg
     1501 ccgagaacgc ggcgatcgag accggggccg acccccggga gaacaccgag gctcagatcg
     1561 ccaccatgac c
//
LOCUS       DATITW010000619         1730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_551478, whole genome shotgun sequence.
ACCESSION   DATITW010000619 DATITW010000000
VERSION     DATITW010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551478"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..383
                     /locus_tag="VLS86_08650"
     CDS             93..383
                     /locus_tag="VLS86_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012014163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSO50604.1"
                     /translation="MGDWNFLTNHARALVFIATEPDSRLRDIAVALDVTERTAFGIMN
                     DLTEAGYVAKEKDGRRNRYHIQAHIPIRDPIGRERAIGELVDLFGNPRPAEA"
     gene            complement(436..>1730)
                     /locus_tag="VLS86_08655"
     CDS             complement(436..>1730)
                     /locus_tag="VLS86_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSO50605.1"
                     /translation="ALAPRHKKQVAVLFLDLDGFKHINDSLGHPVGDMLLQSIAKRLV
                     GCVRTSDTVSRQGGDEFVVLLSEAEQWEDAAIVAARMLIAVAEAHLIENHDLHVTTSI
                     GVSVYPDDGQDAETLIKNADTAMYQAKENGRQSYQFFKPAMNVRAVERQSIEEDLRRA
                     LERHEFVLNYQPKINLRTGAITGAEALIRWSHPTRGVVSPAQFIPIAEESGLIRPIGD
                     WVLREACQQARSWVESGLPPTSMAVNVSATELRDETYVQRLYATLSRTEMDPRSLELE
                     LTESVLMKHAHSAAIILQALRRSGVQVALDDFGTGYSSLSYLRDFPIDALKIDQSFVS
                     EVTSAGEDTSIVTAVISMARSLGLRVVAEGVETQEQLEFLQAHECDEGQGYYFSHPVP
                     PDTFADLLRTGIRLDFDTARSANPFPESTSSVAGVANA"
BASE COUNT          317 a          558 c          538 g          317 t
ORIGIN      
        1 ggcgacccgg ccccggccgt tgtgctcgac tctatatcgg tagatcaaaa ctggcaattt
       61 gacacacgaa tcaagatgct agtgttcgcg aaatgggtga ctggaacttc ctgaccaatc
      121 acgcccgcgc cctggtgttc atcgcaaccg agcccgattc ccggctccgc gacatcgcgg
      181 tcgctctcga cgtcacggag cgaactgcct tcgggatcat gaacgatctc accgaggcgg
      241 gctatgtggc gaaggagaag gacggtcggc gcaaccgcta ccacatccag gcccacatcc
      301 cgatccgcga cccgatcgga agggagcgtg ccatcggcga gctcgtggat ctgtttggca
      361 acccgagacc ggccgaggcg tgactcgcac caagtgatga gggtggccgt ttccgaccag
      421 gtctccgacg ccgtctcagg catttgcgac ccccgccacc gacgatgtcg actccgggaa
      481 cgggttggcg gaccgggcag tatcgaagtc caagcggatg ccggtccgca gcaggtcggc
      541 gaaggtgtcg ggtgggaccg gatgactgaa gtagtagccc tgcccctcgt cgcactcatg
      601 agcctgaagg aactcgagct gctcctgggt ctccacccct tcggccacta cccggagacc
      661 caagctccgc gccatgctga tcacggcggt gacaatgctg gtgtcctccc cggccgaggt
      721 gacctcactg acgaatgact gatcgatctt gagggcatcg atcgggaaat cccggagata
      781 gctcagactg gaatagccgg tgccaaagtc gtcgagtgcc acctgcacac ccgacctccg
      841 cagagcctgc aggatgatcg ccgccgaatg ggcgtgtttc atcaggacgc tctcggtcag
      901 ctccaattcc aacgatcggg gatccatttc cgtccggctc agggtcgcgt agagacgctg
      961 gacgtaggtc tcgtcgcgca actcggttgc ggacacgttc accgccatgc tcgtcggcgg
     1021 cagaccggac tcgacccagg atcgggcctg ctggcacgcc tcgcgcagca cccagtcccc
     1081 aatcgggcga atcaggccgc tctcctcggc gatggggatg aactgggcag gggacaccac
     1141 tccccgggtg ggatggctcc accgaatgag tgcctccgcc ccggtgatcg ccccggtcct
     1201 gaggttgatc ttgggctggt agttgaggac gaactcatgt cgttccaggg cgcggcgcag
     1261 gtcctcctcg atcgattggc gctccacggc ccggacgttc atcgccggct tgaagaactg
     1321 ataactctga cggccgttct ccttggcttg atacatcgcg gtgtcggcat tcttgatcag
     1381 ggtctccgcg tcctgaccgt cgtccggata cacgctgaca ccgatgctgg tcgtgacgtg
     1441 gaggtcgtgg ttctcgatca aatgcgcctc ggccaccgcg atcagcatcc tggcggcgac
     1501 gatggcggcg tcctcccact gctcggcctc ggagagaagc accacgaact cgtcaccacc
     1561 ctggcggctg accgtgtccg aagtgcggac acagcccacg agtcgtttgg cgatcgactg
     1621 gagcagcatg tcaccgaccg ggtggcccag cgagtcgttg atgtgcttga acccgtcgag
     1681 gtccaagaac aacaccgcga cttgtttctt gtgccgtggc gccagggcga
//
LOCUS       DATITW010000620         1588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_310845, whole genome shotgun sequence.
ACCESSION   DATITW010000620 DATITW010000000
VERSION     DATITW010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310845"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VLS86_08660"
     CDS             <1..498
                     /locus_tag="VLS86_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HSO50606.1"
                     /translation="IDTIYEGTTGIQALDLFFRKISRDQGNTLAALASEITEFVKGGL
                     GDDPLAEEREKLARMLDDAQTQLGSMVENLMASFSGESAEIYKVGLHTNSLLESLAEV
                     VIAWQLLRHAEIAIGRVDEDPFYAGKVESARWFLRLAGPQIAARREAATAEDGSLMRV
                     PVEAF"
     gene            535..981
                     /locus_tag="VLS86_08665"
     CDS             535..981
                     /locus_tag="VLS86_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50607.1"
                     /translation="MSGPAVFAFIAVLIGVLLVVVALVVWQEARRRPSYEPLTYIVDD
                     AVKHVEQGLIEEGHHRLSRSDIRRILDWEVFYLQGLAQDDRSNPVETVAGGHEASIDY
                     IIDEIAAKHGVSYAREDVENVLRMEADYLFQIGAVGDVVEFEGEEE"
     gene            1364..>1588
                     /locus_tag="VLS86_08670"
     CDS             1364..>1588
                     /locus_tag="VLS86_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50608.1"
                     /translation="MSRLGRFEHYRYLGARDTMTFYDCDDESQFEDLKTMVEAEDLVG
                     RNRLQAFAPDTPEEAANRGYHAVAAEGEPNA"
BASE COUNT          293 a          488 c          518 g          289 t
ORIGIN      
        1 atcgacacca tttacgaagg gaccaccggg atccaggccc tggatctctt cttccgcaag
       61 atctcgcggg accagggcaa cacgctggcc gcacttgcct ccgagataac cgagttcgtc
      121 aagggtgggc tcggagatga ccccctggcc gaggagcgag agaaactcgc caggatgctc
      181 gatgacgctc aaacccaact cgggtcgatg gtcgagaacc tgatggcatc gttcagcggg
      241 gagtcggcgg agatctacaa ggtgggtctt cacaccaact ccctcttgga gtcgctggcc
      301 gaggtggtga tcgcctggca gctcctgcgt catgccgaga tcgcgattgg tcgcgtcgac
      361 gaggatccgt tctatgcggg caaggtggag tcggctcggt ggttcctccg gctggcgggg
      421 ccacagatcg cggcccgacg cgaagcggcc accgccgagg acggctcgtt gatgagagtg
      481 ccggtcgaag ccttctagct cccgttcacc gtccaccgtt taccgttcac cgtcatgtcc
      541 ggtcccgcgg tcttcgcttt catagccgtt ctgatcggcg tcctcttggt ggtggtggcc
      601 ctggtggtct ggcaggaggc gcgtcgtcgt cccagctacg agcccttgac ctacatcgtc
      661 gacgacgcgg tgaaacacgt ggaacagggt ctgatcgagg agggccatca ccgcctatcg
      721 cgttccgata tcaggcggat cctggactgg gaggtgttct atctccaggg tctggcccaa
      781 gacgaccgat ccaacccggt ggaaacggtt gccggggggc acgaggcatc gatcgactac
      841 atcatcgacg aaatcgccgc taaacacggt gtctcctacg ctcgtgaaga cgtggagaat
      901 gtgctccgca tggaggccga ctatctgttt cagatcggcg ccgtgggaga cgtggtcgag
      961 ttcgaaggag aagaggaatg atcgtccgtt gcggcgcatg ccggacccag ttcgaggtcg
     1021 ccggtgccgg tcgattctcg tgcccgtcct gtggctctgt gaacgtggtt cgcaaccaaa
     1081 cggcgacggg cccggcatcg gcgggggcct accccacggc ccctggtgcc ccggcaaccc
     1141 cagccgcgcc accaccagac cgtccctcgc cccggatcaa ttgtcccgag tgcgccttca
     1201 ccttcattgt gggcgatatt gctgtcgcca cctgtcccaa ctgcggcacc gaggtccaga
     1261 ccgggaaggg tgtcccatcg gcaccgatgg aggcggttcc cgaggagccc gtcacagtcg
     1321 gcgctccaac ggaagatgaa gacccggtgt tgacggaaga ggaatgagcc gtctcggtcg
     1381 cttcgagcac tatcggtacc tgggggcgcg ggacacgatg acgttctatg actgtgacga
     1441 cgagtcacag ttcgaggacc tcaagaccat ggtcgaagcc gaagacctgg tggggagaaa
     1501 ccggctccaa gccttcgccc cggacactcc ggaggaagcg gccaatcggg gttatcacgc
     1561 ggtggccgcg gagggcgaac cgaatgcc
//
LOCUS       DATITW010000621         1761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_591456, whole genome shotgun sequence.
ACCESSION   DATITW010000621 DATITW010000000
VERSION     DATITW010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591456"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VLS86_08675"
     CDS             complement(<1..444)
                     /locus_tag="VLS86_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50609.1"
                     /translation="MIFAVLLLLSSSMVSPPLSDQPLGEISAFYSSNRAVVIVAQITG
                     VIAAIAFALFVTMLSTVLDAPQVKWTGLVVAAAAAVTSVPLLALASLGANADPGIVRW
                     TDLTDAVLFLAIAVFFGVLAGNGALPSWIRGLCVIGAGLTLVRGYL"
     gene            complement(505..>1761)
                     /locus_tag="VLS86_08680"
     CDS             complement(505..>1761)
                     /locus_tag="VLS86_08680"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSO50610.1"
                     /translation="RVVFDTDEAGIIDIAVKGAEHCRAQEQFLPESEIRYEYSPESFT
                     GTALPFAKRVCEAVMDVIEPTAERPIILNLPATVEMSTANLYGDMIEWFHREIKNRPA
                     VVLSLHPHNDRGTAVAAAEFGLMAGAQRVEGTLFGNGERTGNVDVVTLALNLFSQGVD
                     PELELNNINEYRRIAEHCNQLPVHPRHPYVGDLVYTAFSGSHQDAIKKGFEAMDRTGQ
                     ALWEVPYLPIDPRDVGRTYEDVIRVNSQSGKGGVAYLLKTEQELEIPRRLQIEFTRVI
                     QGITDTTGHEITAAEIWTEFRRHYLAILKPFELLGFEISRDAEIENRSNITATVRYND
                     EEHVIRSDGGGSIEAFVNAFKKDLGVVCRVLDYHEHSIGAGTDAQAVCYVEMEVGERT
                     LWGVGIHSDTTTASLQAIVSGINRAS"
BASE COUNT          340 a          548 c          557 g          316 t
ORIGIN      
        1 aaggtaccca cgcaccagag tgagtccagc cccgatcaca caaaggcccc gaatccaact
       61 cggaagcgct ccgttgccgg cgagcacgcc aaagaaaaca gcgattgcca ggaacagcac
      121 tgcatcggtg aggtcggtcc atcggacgat gcctgggtct gcgttagcgc ccaaagatgc
      181 gagagcaagc agcggcaccg aggtgaccgc cgcagccgcg gccaccacca aaccggtcca
      241 cttcacttgg ggagcatcga ggactgtgga gagcatggtg acgaagagag cgaaggcgat
      301 cgcggcgatg accccggtga tctgggccac gatcaccacc gctcggttcg acgagtagaa
      361 ggcgcttatc tcaccgagcg gttggtccga aagtggaggg ctgaccatcg aggaggagag
      421 caacaagagg acggcgaaga tcacccccaa ccatccgacc cgacgctggc tcgcgttggc
      481 gacggtggtg acctcctccg actgtcagct ggcgcggttg ataccggaga cgatcgcctg
      541 cagggaggcg gtcgtggtgt cggagtggat gccaaccccc cagagggtgc gctcgccgac
      601 ttccatctcg acgtagcaca ccgcctgagc gtcggtcccg gcgccgatcg agtgctcgtg
      661 atagtcgagg acccggcaaa ccacaccgag atctttcttg aaggcattga cgaaggcctc
      721 gatcgacccg ccaccgtcgc ttcggatcac atgctcctcg tcgttgtatc gcacggtggc
      781 cgtgatgttg gatcggttct cgatctcggc atcccgagag atctcgaatc cgaggagctc
      841 gaagggcttg aggatcgcca gatagtgacg gcgaaactcg gtccagatct cggcagcggt
      901 gatctcatgc ccggtggtgt cggtgatgcc ctggatgaca cgggtgaact cgatctggag
      961 acggcgcggg atctcgagct cctgctcggt cttgagcagg taggcgaccc cgcccttgcc
     1021 cgattgcgag ttgaccctga tcacgtcttc gtaggtgcgc ccgacatcac gggggtcgat
     1081 gggaaggtag ggcacctccc aaagggcctg cccggtccga tccatcgcct cgaacccctt
     1141 cttgatcgcg tcctgatgtg agcctgagaa ggcggtgtag accaggtcac cgacataagg
     1201 gtgacggggg tggaccggca gctgattgca gtgctcggcg atcctccggt actcgttgat
     1261 gttgttcagc tcgagctcgg gatctactcc ctggctgaac aggttgaggg ccagcgtcac
     1321 cacgtcgaca tttccggtcc gctctccgtt gccaaacagg gtgccctcca cacgctgggc
     1381 gcctgccatc aaaccgaact cggctgcggc gaccgcggtg ccacggtcat tgtgtggatg
     1441 cagggacaag accaccgccg gtcggttctt tatctcacga tggaaccact cgatcatgtc
     1501 cccgtagaga ttcgccgtgc tcatctcgac ggtcgccggc aagttgagga tgatcgggcg
     1561 ctcagcggtc ggctcgatca catccatcac cgcctcgcag actcgcttgg cgaagggcag
     1621 tgcggtgccg gtgaagctct caggcgagta ctcgtagcgg atctccgatt ccggcaagaa
     1681 ctgctcctgg gcccggcagt gctcagcccc cttcaccgcg atgtcgatga tgcccgcctc
     1741 gtcggtgtcg aacaccaccc g
//
LOCUS       DATITW010000622         1804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_551754, whole genome shotgun sequence.
ACCESSION   DATITW010000622 DATITW010000000
VERSION     DATITW010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551754"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..807)
                     /locus_tag="VLS86_08685"
     CDS             complement(52..807)
                     /locus_tag="VLS86_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013045793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSO50611.1"
                     /translation="MTGGSRGIGKAIARRFAEEGADVVISSRTESDLESTAKEISSAT
                     GRRVELVAADLSTLEGCESLHQGALAAFDHIDILINCAGATKSGPFLELSDELWREGF
                     DLKFFGAVRLSRLFWPQLVETHGSVVHIIGGMARTPNPVFAIGGAVNAALANFGKALA
                     GVGLEDDVNVNMIHPGQTVTERLDEMMADQAARSGQTPEEVLEATVKRQGIRRLGTPE
                     DVAELALFLCLPRARHIQGTAISIDGGATKGLF"
     gene            909..>1804
                     /locus_tag="VLS86_08690"
     CDS             909..>1804
                     /locus_tag="VLS86_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005451145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HSO50612.1"
                     /translation="MRIAVLDDYQRVAESLANWSELDAEVEFLHDHISDRPALLTRLS
                     GFDVVVAMRERTPFPRDVLEGLPDLRLLVTTGSANASIDTAAANELGIVVSGTGSLGH
                     GTAELTMALLLALARGLLPEIDSVRRGGWQVGLGRDLLGSTLGVIGLGRLGAQVASLG
                     LAFGMEAIAWSQNLTRDRAGEVGVLAVSKEELLGRSDFVTIHLKLSDRTRGLIGEPEL
                     SSMKPSAFLVNTSRGPIVSEDALLSAVGAGVIAGAAVDVYDHEPLPSDHPFRTHPRIL
                     ATPHIGYVTRESYEVFFRGAVES"
BASE COUNT          287 a          607 c          583 g          327 t
ORIGIN      
        1 atgtcggtgc gttgtgcgtg atcggtcaag tctggtcaac ctcgagtcgt ctcagaacag
       61 ccctttggtc gctccgccgt cgatggagat ggcggttcct tggatgtggc gggcccgggg
      121 aagacagagg aacagcgcca gctcggcgac atcctccgga gtgccgagac gtcggattcc
      181 ctgacgtttc accgtcgcct ccaggacctc ctcaggagtc tgcccgctcc gggcagcctg
      241 gtcggccatc atctcgtcca gtcgctcggt gaccgtctgc ccggggtgga tcatgttcac
      301 attgacgtcg tcctccagac cgacaccggc cagagccttg ccaaagttgg ccagggcagc
      361 gttgactgcg ccaccgatgg cgaagacagg attcggggtc cgcgccatac cgccgatgat
      421 gtggaccacg gatccatgag tctcgacgag ttggggccag aagagacgag agagacgaac
      481 cgctccgaag aacttgagat cgaagccttc tctccacaac tcatcggata gctcgaggaa
      541 gggcccgctc ttggtggccc cggcgcagtt gatcaggatg tctatgtggt cgaaagcagc
      601 gagtgcgccc tggtgcaggc tctcgcagcc ctccagagtc gacaggtcgg ccgcgaccag
      661 ctccacccgg cgcccggttg cggatgagat ctccttggcg gtcgactcca ggtccgactc
      721 ggttcgggag gagatcacga catctgcccc ctcctcggca aaacgccgcg cgattgcctt
      781 cccgatgccc cggctgccac cggtcaccac agcaaccttg cctgccaact ccacaaacac
      841 ctcctggacg ccgcctggat cggaggttaa cgagggccac gggacctccg cggctaccgt
      901 gcgatgccat gcgcatcgcg gtcctggacg actatcagcg ggtcgccgag tcgctcgcca
      961 actggtccga gctcgacgcc gaggtcgagt tcctccatga ccacatctcc gaccggccgg
     1021 cgctgctgac acggctttcc ggcttcgacg tcgtggtcgc gatgcgggaa cgaaccccat
     1081 ttccccgcga tgtcctggag ggcctccccg atctccgtct cctggtcacg accgggtcgg
     1141 ccaacgcctc gatcgacacc gcggctgcga acgagctcgg catcgtcgtc tccgggaccg
     1201 ggtcgctcgg tcacgggacc gccgagttga cgatggcgct gctcctggct ttggcccggg
     1261 gcctcctgcc ggagatcgac tcggtgcgcc gggggggctg gcaggtgggt ctggggcggg
     1321 atctcctcgg ttcgaccctg ggcgtgatcg gtcttggtcg gctcggggcg caggtggcga
     1381 gtctcgggct cgccttcggc atggaggcca tcgcgtggag tcagaacctg accagagaca
     1441 gagccggcga ggtcggcgtc ctcgccgtgt ccaaggagga gttgctcggg cgctccgact
     1501 tcgtgaccat ccatctcaag ttgagcgacc ggactcgcgg cctgatcggt gagcccgagc
     1561 tctcctcgat gaagccaagt gcgtttctgg tcaacacctc acgtggcccg atcgtgagcg
     1621 aggacgccct gctctccgcg gtcggggcgg gcgtcatcgc cggagccgcg gtcgatgtct
     1681 acgaccacga gccgttgccc tcggaccacc cgttccgcac ccacccccgg atcctggcca
     1741 ccccccatat cggatatgtg acccgagagt cctacgaggt cttctttcgg ggggcagtcg
     1801 agtc
//
LOCUS       DATITW010000623         1555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_631799, whole genome shotgun sequence.
ACCESSION   DATITW010000623 DATITW010000000
VERSION     DATITW010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631799"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1470)
                     /locus_tag="VLS86_08695"
     CDS             complement(79..1470)
                     /locus_tag="VLS86_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HSO50613.1"
                     /translation="MTATASPPPITRVSRIDWIKSNLFPNPWSGLLTIVLAPIVLYMA
                     YRASMFLFVNGRWEAVERNLTLFMQGTFPRDQQWRLVAQLTLFALSLGVGLGTASASA
                     RDRAMEAGLPHERSTPLDAIRRFWSVILFVLVLLYFAATTTPWLVVLGAVVALLIGLQ
                     VYRLPISLRLYGWLLMALFLVAGFQVVSGTTGRAWVVTALVVGIGAWSLVESERIPPG
                     WGTWALKTGAVVMSTVIVFLVYSAFPIEGLGWDTWSGLHLTLWVSALAIILSFPFGLM
                     LALARRSGLPALRLLATTYIELIRGVPLIALLFMGQYVAGFLLPAGTTLSSISRAIGV
                     FTLFTAAYVAEIVRGGLQSLPPGQAEAGLALGLSPPSVIRLIVLPQALRAVIPAMVGQ
                     FISLFKDTSLLSILAIFEFFNVRSLIHAQPEFRGIAHAETLVFVAFGYWAIAFAMSRE
                     SQRLEGRLRVGEA"
BASE COUNT          374 a          483 c          496 g          202 t
ORIGIN      
        1 ctaccccctc caactcgatg atcacgtcgc ctttggccct ggtggcgtcg gcgaacacct
       61 ccgggctcga ttcaaagctc atgcttctcc aactctcagt cgaccctcca gccgctggct
      121 ctcacgggac atggcaaagg cgatcgccca gtagccgaaa gcgacgaaaa ccagggtctc
      181 ggcatgggcg atgcctcgaa actcgggctg ggcatggatc agcgaccgca cattgaagaa
      241 ctcgaagatg gcgagaatcg acagaagtga agtgtccttg aacaaggata tgaactgccc
      301 caccatcgcc gggatcacag ctcgcagcgc ctgggggaga acgatgagac ggatcaccga
      361 cgggggcgac aggccgagcg ccagaccggc ctcggcctga cccgggggca gtgactgcag
      421 cccaccgcgg acgatctcag ccacgtaggc cgcggtgaac agggtgaaga ccccgatcgc
      481 ccgggagatg ctggacagcg tcgtccccgc agggagcagg aatccggcca cgtactgacc
      541 catgaagagc agggcgatca ggggaacccc tcgaatcaac tcgatatagg tggttgccag
      601 caaccgaagc gccggaagac cggagcgacg ggccagcgcc agcatcaagc cgaagggaaa
      661 ggagaggatg atggcgagtg ccgacaccca aagcgtgaga tgcaggcccg accacgtgtc
      721 ccatcccagc ccctcgatcg ggaacgccga atagacgagg aacacgatga ccgtggacat
      781 gaccacggct cccgtcttca gagcccaggt cccccacccg ggtgggatgc gctcgctctc
      841 gacgagggac cacgccccga tgccgacaac cagcgcggtg accacccagg cgcggccggt
      901 ggtccccgac accacctgaa acccggcaac gaggaacagt gccatcagca accagccata
      961 gagccgcaat gagatcggca agcggtacac ctggagcccg attagcaatg ccaccaccgc
     1021 tccgagcacc acaagccacg gggtggtggt ggcggcaaag tagagaagca ccaggacgaa
     1081 gaggatcacc gaccagaacc ggcggatggc atccagcggg gtgctccgct catgagggag
     1141 acctgcctcc atggcccggt cacgagccga cgccgaagcg gtgcccagcc ccaccccgag
     1201 tgacagggcg aacaaggtca actgggcgac gagacgccat tgctggtcgc gggggaacgt
     1261 cccctgcatg aacagggtca ggttgcgctc gaccgcctcc cacctcccgt tgacgaacag
     1321 gaacatggat gcccgatagg ccatgtacaa gacgattgga gcgagaacga ttgtcagcag
     1381 acccgaccat gggttgggaa acaggttgga cttgatccag tcgatccggg agacacgtgt
     1441 gatcggaggt ggggaggcgg tggcagtcat cgtgtgacca gctggaagcg atgattgacg
     1501 aagttgagca gcagtgagat ggagagggag aacatcagat acacccccat catca
//
LOCUS       DATITW010000624         2795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_591722, whole genome shotgun sequence.
ACCESSION   DATITW010000624 DATITW010000000
VERSION     DATITW010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591722"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VLS86_08700"
     CDS             complement(<1..249)
                     /locus_tag="VLS86_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50614.1"
                     /translation="MKNESVFWVMAAAVGAGFGLLVAFGASGAAIILMIGLGILSISA
                     VGRTMRREVDEKWLASWIVLGFVAKMAGTYARYYMVMYL"
     gene            complement(246..1193)
                     /locus_tag="VLS86_08705"
     CDS             complement(246..1193)
                     /locus_tag="VLS86_08705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSO50615.1"
                     /translation="MPEIATREAKPEDVDEILRVLRAALGETPVLRRTPELWAWKHES
                     NPFGPSIVLLATSDDRIAGVRALMQWQLTTPEGATIRCVRPVDTATHPGFERQGIFRR
                     LTMESLDLARERGIDLVFNTPNPQSGAGYLSMGWREVAPIGVMVRPRVGKAVPAVPGS
                     APSLSEVIPAATPFRAVEAPDRPPRGLRTRRDHAYQDWRFGSHPTVRYGLVGSERGAA
                     VVRAGVRAGRTELVVSDLLGEAGARNLRRSHRAHRARYMAGFFSKGSPERAAAMAAGM
                     IPVPGMKALRLVALPLSDVDIDVFDLGFWDIATSDLELL"
     gene            complement(1186..2295)
                     /locus_tag="VLS86_08710"
     CDS             complement(1186..2295)
                     /locus_tag="VLS86_08710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSO50616.1"
                     /translation="MSSDRADRTRILVLIKGLGIGGAERLISDGAEHWDRDRFEYRVA
                     YVLPWKDHLVEPLRRLGVEVDCVGSKRGMGPTALRGIRRLVRVWRPGLIHAHLPSAGI
                     LARVSGSAPVVYTEHNIASSYRQPTRSLNRLSYGRNAAVIAVSAAVADSLQGYPGPTP
                     RVIPNGITFQVTATEVAAVREELGLVPGQRLVVHVGNIRPHKGHENLIAATKRIVKAD
                     PNVLVVSVGAEKHEGDLARVRESAAVAGVSEHIRFLGRREEARAFLAAADAVVNPADF
                     EGLPLAVLEALSLSKPVVATAVGGVPTVVIDGVTGRLVPPGDAEALADGVLEALSSPD
                     AGLWGAAGAELVSRNHGIAQMISEYEKTYEEVAGA"
     gene            complement(2276..>2795)
                     /locus_tag="VLS86_08715"
     CDS             complement(2276..>2795)
                     /locus_tag="VLS86_08715"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSO50617.1"
                     /translation="EEHPTMPDHPYSITKLAGESLVLSAADLRSLSAVALRLGTAYGS
                     GLRPNSVFRIFIDRAQKGEPITIQGDGRQGRQFTHASDIANAFFLAAQSEVSGVAMNI
                     VAPDTVSVRELAERVVERFPTELTFGEPRPGDVPPALVSADRAKQLLGWEAVMPFDQG
                     LDELIADVLGSS"
BASE COUNT          480 a          980 c          885 g          450 t
ORIGIN      
        1 gaggtacatc accatgtagt accgggcgta ggtgccggcc atcttggcga cgaagccgag
       61 gacgatccag ctcgccagcc acttctcgtc gacctcgcgt cgcatggtgc gacccaccgc
      121 cgatatggag agaatgccga gcccgatcat caggatgatc gccgctcccg aggcgccaaa
      181 ggccacgaga agcccgaaac cggccccgac cgccgcggcc atgacccaga acaccgactc
      241 gtttttcaca acaactccag atcgctggtg gcgatgtccc aaaagcctag gtcgaatacg
      301 tcgatgtcaa catcgctcaa cggcagtgcc acgagccgga gagccttcat ccccggcacc
      361 gggatcatcc ccgcggccat cgccgctgcc cgctccggcg agcccttgga gaagaagcct
      421 gccatgtacc gggcccggtg ggcgcgatgc gaccggcgca gattgcgggc gccggcctcc
      481 ccgaggagat cggacaccac caactcggtc ctgcccgccc tgactccggc ccgtaccacc
      541 gctgctcccc gctcggatcc gacgagacca tatcggactg tgggatgact gccgaaacgc
      601 cagtcctggt aggcgtggtc acgtcgcgtc ctcagccccc gcggcgggcg gtcgggcgcc
      661 tcgacggctc gaaacggggt ggcggccggg atcacctcgg agagcgaagg cgctgatccg
      721 ggaaccgcgg gaaccgcctt cccgactcga gggcggacca tgacacctat cggcgccacc
      781 tctcgccagc ccatcgacag atagccggcg cccgattgcg ggttgggggt gttgaacacc
      841 aggtcgatcc ctcgttcccg ggccagatcg agtgactcca tcgtgagcct ccggaatatc
      901 ccctgacgct cgaagccggg atgcgtcgca gtgtccacgg ggcgaacaca tcggatggtt
      961 gcgccttccg gcgtcgtcag ctgccattgc atcagtgccc tgacccctgc gatccgatcg
     1021 tctgaggtcg ccaggaggac gatcgaggga ccaaagggat tcgactcgtg tttccatgcc
     1081 cacagctccg gagtccgacg gaggacaggt gtctccccga gggccgccct cagcacacgc
     1141 aggatctcgt cgacgtcctc gggtttcgcc tcgcgggtcg cgatctcagg caccggcgac
     1201 ctcttcgtag gtcttctcgt actccgagat catctgagcg atcccgtggt ttcgtgacac
     1261 cagttccgct ccggccgccc cccataggcc ggcgtccggc gacgacagtg cctcgaggac
     1321 gccatcggcc agagcctccg catcgcccgg tggcaccaga cgcccggtca ctccgtctat
     1381 caccacggtc gggaccccgc ccaccgccgt ggcaaccacc ggcttgctca gagacagggc
     1441 ctccagcacc gccagcggca gcccttcgaa gtcggcgggg ttgaccacgg cgtctgctgc
     1501 cgcgagaaag gctcgcgctt cctctcgacg tcccaggaac cggatgtgct cggagacccc
     1561 ggcgacggct gcagattccc gcacccgtgc caaatccccc tcgtgcttct cggcgccgac
     1621 cgacaccacc agcacgttcg ggtcggcctt gacgatccgc ttggtggcgg cgatcaggtt
     1681 ctcgtgcccc ttgtgggggc ggatgttgcc cacgtggacc accagccgct ggccggggac
     1741 caggcccaac tcttctcgca ccgctgccac ctcggtcgcc gtcacctgga acgtgatccc
     1801 gttcggtatc actctgggtg ttggccccgg gtagccctgg aggctgtcag ccaccgcggc
     1861 ggagaccgcg atcaccgccg cgtttcgccc atagctgagc cggttgaggc tgcgggtggg
     1921 ctgccggtat gagctcgcga tgttgtgctc ggtgtagacc accggggccg agccggagac
     1981 tcgagcgagg atcccggcgg aagggagatg agcgtggatc aagcccggtc gccagacacg
     2041 aaccagtctg cgaatgccac gaagcgcggt cgggcccatt ccccgtttcg agcccacaca
     2101 gtcgacctcc acgccgagcc ggcgcaacgg ctcgaccaga tggtccttcc agggcagcac
     2161 ataggcgacc cggtattcga atcgatcgcg atcccaatgc tcggcaccgt ctgagatgag
     2221 acgctcagcg ccaccgatcc ccagaccctt gatcaggacc aggatgcggg tgcggtcagc
     2281 tcgatccgag gacatcggcg atcaactcat ccaggccctg gtcgaaaggc atgactgcct
     2341 cccaacccaa gagctgcttg gcccggtctg cggacacgag ggcgggcggc acgtcgcctg
     2401 gcctgggctc gccgaacgtc aactcggtgg ggaacctctc gaccacccgc tccgccaact
     2461 cccgaacact gacggtgtcg ggggcgacga tgttcatggc gacacccgag acctcggatt
     2521 gggccgcgag gaagaaggcg ttggcgatgt ccgaagcgtg ggtgaactgt cgcccctgcc
     2581 tcccgtcgcc ctggatggtt atcggctcgc ccttctgggc gcggtcgatg aatatccgga
     2641 acacggagtt gggtcgcaga cctgatccgt aggcggtgcc cagtctcagc gcgaccgcgg
     2701 agagtgaccg aagatcggcg gcgctgagca ccagcgactc tcccgccagc ttggtgatgc
     2761 tgtacgggtg atcgggcatc gtcggatgct cttcg
//
LOCUS       DATITW010000625         1548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_511032, whole genome shotgun sequence.
ACCESSION   DATITW010000625 DATITW010000000
VERSION     DATITW010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511032"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VLS86_08720"
     CDS             <1..534
                     /locus_tag="VLS86_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50618.1"
                     /translation="LLLGLVSHGETRFLFFPLALVVVAGSLSVSDWVTGVGKPWSHVI
                     AWVTSILVIGSLALSAPEARQSVENRSENNEAIEVAAHAVADMAGGQPCGVLTSYTPQ
                     MTFYSACASEIFEGELGPQESLAGLRGDVRFMVLIEGGKRQPVGEDLRGFIDLTTGPP
                     VVIDTANGGGLIYQFQD"
     gene            590..1279
                     /locus_tag="VLS86_08725"
     CDS             590..1279
                     /locus_tag="VLS86_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50619.1"
                     /translation="MPGDLTTLAQPQLVLVAWAGGLALAAGFVALARVVGPGFTWLSS
                     SVAVLIGLAGAFGDDTWWARLGLLLLGLGMIWARNNLMAGVFQTVAGVALVVQAAQIG
                     GWLPAVSVSIALGGVTSEMLLGHWYLVDPRLPRWALRALALVGIVGLAADAGVLAMAG
                     LPGGGATVAYWALMGTSLVLMAGVIGSLRYPAYAGVMAATGLSYLAVLTTLGGVFLGR
                     VLVAGVGPFAN"
     gene            1289..>1548
                     /locus_tag="VLS86_08730"
     CDS             1289..>1548
                     /locus_tag="VLS86_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HSO50620.1"
                     /translation="MVEIRTTSAEVELTDATLIVGVLVGPIPTPGGEEALAAVDPALV
                     EVAKFEGKTGQVLAVPHPAAQSLILVGLGDEASFETVRAAIG"
BASE COUNT          211 a          483 c          554 g          300 t
ORIGIN      
        1 ctccttctcg gcttggtatc gcatggcgaa acgaggtttc tcttctttcc ccttgccctg
       61 gtcgtagtcg ccgggtcgtt gtcagtgagc gattgggtaa ccggagtcgg aaagccgtgg
      121 tcccacgtca tcgcctgggt cacgtcgatc ctggtgatcg gctcactggc gctttcggct
      181 cccgaggcca ggcagtcggt ggagaaccgc agcgagaaca acgaggcgat cgaggtcgcc
      241 gcccacgccg ttgccgacat ggccggggga cagccgtgcg gggtgctcac ctcctacacg
      301 ccacagatga ctttctactc ggcttgcgcc agcgagatct tcgaagggga actgggtccc
      361 caggagtcgc tggcaggtct tcgaggcgac gtccggttca tggttctgat cgaaggagga
      421 aagcgtcaac ccgtcggaga ggatctccgg ggtttcatcg acctcaccac aggaccgccg
      481 gtggtcatcg acactgccaa cgggggtggg ctcatctacc aattccagga ttgagccggc
      541 acggcgccag cggacgggct cgcgctctcc ggtcataaca tgcgccgccg tgcccggcga
      601 cctgaccacc ctcgcccaac cccaactggt cctggtcgcc tgggcaggtg ggttggcgct
      661 tgccgccggt ttcgtcgccc tcgcccgggt ggtggggccc gggttcacct ggctgtcgtc
      721 gtcggtggca gtgttgatcg gtctggccgg ggcgtttggc gatgacacct ggtgggcgcg
      781 gctcgggctg ctgcttctcg gtctcgggat gatctgggcg cgcaacaacc tgatggcggg
      841 ggtcttccag acagtggccg gtgtggcgtt ggtggtgcag gccgcacaaa tcggcggatg
      901 gctgccggcc gtctcggtct ccatcgccct gggaggggtc acctccgaga tgctgttggg
      961 tcactggtac ctggtggacc cgagactccc ccgctgggct ctgcgcgccc tggcgctggt
     1021 ggggatcgtc gggctggccg ccgacgccgg tgtcctcgcc atggccgggc tccctggcgg
     1081 tggggcgacc gttgcctatt gggccctgat gggcacgtcc ctggtgctca tggccggagt
     1141 catcggttcg ctccggtacc cggcgtacgc cggggtgatg gccgccaccg gcttgtcata
     1201 tctggccgtg ctcaccactc tgggaggagt cttcctcggg cgagtgctgg tcgccggtgt
     1261 gggacctttc gccaactgaa taggagacat ggtggagatc aggaccacgt cagctgaagt
     1321 cgagctcacc gatgccacgt tgatcgtggg cgtcctggtg ggcccgatcc cgaccccggg
     1381 cggcgaggag gccctcgcgg ctgtcgaccc ggcattggtc gaggtggcga agttcgaagg
     1441 aaagacgggt caggtgctgg cggtgcctca cccggccgcg caatcgctga tcctggtggg
     1501 gctgggcgac gaggcgagct tcgagaccgt ccgcgccgcg atcggcaa
//
LOCUS       DATITW010000626         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_31006, whole genome shotgun sequence.
ACCESSION   DATITW010000626 DATITW010000000
VERSION     DATITW010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31006"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VLS86_08735"
     CDS             complement(<1..364)
                     /locus_tag="VLS86_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50621.1"
                     /translation="MVRHLVGLKARLTWNGFRSDLQRRIGLPVALGLLTWAGIWLAGA
                     HHRTALLLADASPQALGPYLAWSALFLFLIWVTLPVIIFPLDENLDPQQLANLPITPN
                     RMVAGLASASLIAPSTVVP"
     gene            complement(349..1149)
                     /locus_tag="VLS86_08740"
     CDS             complement(349..1149)
                     /locus_tag="VLS86_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009189183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50622.1"
                     /translation="MTQPLIEEPLVDAVETPPAVWAKGLVRKFGKTIAVDHVDLSIGT
                     GEFYGLLGQNGAGKTTTIKTIVGLLRPNQGTVGIGEFNTWEHPTQVKARIGVLPEEFN
                     LYERLTGAELLDFTAAMHGLDRDEAIRRRNELLDLLDLVDAGSKLVGDYSRGMRKKVA
                     LGAAIIHRPPVLFLDEPFEGVDAVSARLIRRLLQQAAQGGTTIVFSSHEMHLVEKLCT
                     RIGIMVAGKLTIEDTPEGLCTKMGVDNVEDAFIAAVGGRTPDSSWYVT"
     gene            1207..>2281
                     /gene="dnaX"
                     /locus_tag="VLS86_08745"
     CDS             1207..>2281
                     /gene="dnaX"
                     /locus_tag="VLS86_08745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HSO50623.1"
                     /translation="MPPAAGTDYAAPWQPVPWGAVEYQALYRKYRPQTFDDVIGQGHV
                     TTTLSREVADGKVAHAYLFTGPRGTGKTTTARILAKALNCENRAANGSPCNTCASCVA
                     ITEGSSLDVLELDAASHNSVDDIRDIKVSVTTVASSATSKRVFVLDEAHMLSKAAGNA
                     LLKTLEEPPQHVHFVLATTEPYKLLDTIRSRSQRFDFHPVPIEDLAGHLSVIADREGY
                     KTDPPALIAIARHAGGSVRDSLSLLEQVSALGAGSVDVPGVRRALGLADSEAYMRLVE
                     AVAAHDAKSALELVAELAADGVDLRRFVAESVSFFRGAFLAHYAPNLAEIADEPPDVY
                     EAWRKAAQIVPAGDVLRAVDLLGE"
BASE COUNT          393 a          744 c          759 g          385 t
ORIGIN      
        1 gcggcaccac ggtcgagggg gcgatcagac ttgccgacgc cagcccggcg accattcggt
       61 tgggggtgat cggcaggttg gcgagctgct gagggtccag gttctcgtcc agggggaaga
      121 tgatgaccgg gagggtcacc cagatcagga acaggaacag ggcagaccag gccaggtatg
      181 ggcccaaggc ctgaggactc gcgtccgcga ggaggagcgc agtccggtga tgcgccccgg
      241 cgagccagat gccggcccag gtgagcagtc ccagggccac cgggaggccg atccggcgct
      301 ggaggtcgct cctgaacccg ttccaggtga gtctggcctt gagaccgact aggtgacgta
      361 ccacgacgag tccggcgttc tgccccccac ggcggcgatg aaggcgtctt cgacgttgtc
      421 gacacccatc ttggtgcaca acccctcggg cgtgtcctcg atggtcagct tgccggcgac
      481 catgatcccg atccgggtgc acagtttctc gaccagatgc atctcgtggg aggagaagac
      541 aatggtggtc ccgccctggg ccgcctgttg cagcagccgt cggatcagtc gggcggagac
      601 ggcgtcgact ccctcgaacg gctcgtcgag gaacaggacc gggggacgat ggatgatggc
      661 tgcccccagc gcgactttct ttcgcatccc cctcgagtag tccccgacca gcttcgaccc
      721 ggcatcgacc aggtcgagca ggtcgagcaa ctcgttccgt cgcctgatcg cctcatcacg
      781 atcgagaccg tgcatcgccg cggtgaagtc gagcagctcg gctccggtca accgctcgta
      841 gaggttgaac tcctcgggca agaccccgat acgcgctttg acctgggtgg gatgctccca
      901 ggtgttgaac tcaccgatcc cgactgtgcc ctgattgggg cggagcaggc ccacgatcgt
      961 cttgatcgtg gtcgtcttgc cggcgccgtt ctggccgagc aggccgtaga actccccggt
     1021 gccaatcgac aggtcgacat ggtcgacggc gatggtcttc ccgaacttgc ggaccaggcc
     1081 tttggcccag accgcaggag gcgtctcgac ggcgtcaacc agaggctctt cgatgagcgg
     1141 ttgcgtcacc ttggcagtat cgacggatcg gcccaataaa tgaaccgaaa gtgaggatgg
     1201 cgtcgattgc cgcccgcagc cggaaccgat tacgcagcgc cgtggcagcc ggtaccgtgg
     1261 ggcgcggtgg agtaccaggc tctgtatcgc aagtatcggc ctcagacttt cgacgacgtc
     1321 atcggtcagg gccatgtcac tacgacgctt tcccgtgagg tcgccgacgg gaaggtggcg
     1381 catgcctatc tgttcaccgg gccccggggc accgggaaga ccaccacggc gcggatcctg
     1441 gccaaggcac tgaactgtga gaaccgggcg gccaacggct caccgtgcaa cacctgcgcc
     1501 agctgcgtgg cgataacaga aggctcctca ctggatgtgc tcgagctcga cgcggcatcg
     1561 cacaactccg tggacgacat tcgggacatc aaggtcagcg tgaccaccgt ggcctcttcg
     1621 gccacctcga agcgggtctt cgtgctcgac gaggcgcaca tgctgtccaa ggcggccggc
     1681 aacgcgctgc tcaaaaccct ggaggagcca ccacagcacg tccatttcgt gctggccacc
     1741 accgagccct acaagctgct cgacaccatc cgctcccgca gccagcggtt cgacttccac
     1801 ccggtcccta tcgaggacct cgccggccat ctcagcgtca tcgccgaccg tgagggctac
     1861 aagaccgacc ccccggcgct gatcgccatc gcccgtcacg ccggaggctc ggtccgcgat
     1921 tcactctccc tgctcgagca ggtctcggcg ttgggggcag ggtcggtcga cgtccccggg
     1981 gtgagacgtg cgctcgggtt ggccgattcg gaggcgtaca tgcgcctggt cgaggcggtc
     2041 gcggctcatg acgcaaagtc cgcgttggag ttggtggcag agctggccgc cgacggagtc
     2101 gatctcaggc ggttcgtcgc cgagtcggtg tccttcttcc gcggggcgtt cctcgcccac
     2161 tacgcgccga atctcgccga gatcgcggat gagccacccg atgtctacga ggcgtggcgg
     2221 aaggcagccc agatcgtccc cgccggagac gtgttgcggg cggtcgacct gctcggtgag
     2281 g
//
LOCUS       DATITW010000627         3586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_270984, whole genome shotgun sequence.
ACCESSION   DATITW010000627 DATITW010000000
VERSION     DATITW010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270984"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VLS86_08750"
     CDS             complement(<1..589)
                     /locus_tag="VLS86_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HSO50624.1"
                     /translation="MPGDLGRTLGDEYVNQLPDSDPVETSEWIDSLDAVVAGQGPDRA
                     RFLVAKLIERAHETNVGMAGVITTPYINTIPPEEEPEFPGDEDIERRIRRYIRWNAAV
                     MVIKANHRSKGIGGHLSTFASSATLYEVGFNHFFRGKDAPGGGDHVYMQGHAAPGIYA
                     RSYLEGRFDEKHLDNFRFEIDGDGLSSYPHPRLMPD"
     gene            complement(617..1522)
                     /locus_tag="VLS86_08755"
     CDS             complement(617..1522)
                     /locus_tag="VLS86_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO50625.1"
                     /translation="MTDYALTSMGPMHQVDFGGSGPDLVLVHGLGGSTTNWNAVGPGL
                     ARTARVRAIDLPGFGLTPPRGNFRLNTHRDSIVAYLETLDSSNTTLIGNSSGGLLSEL
                     VAAARPDLVHRLILVSPATPPVFPDPQLDWPTVIRLSVQATPFIGEVYGRHFLRSNTP
                     EELVQKSLAMITHKPGRVPMGLIADSRDMAHIRKQLPWAGYATARTATSIARLYLRRT
                     AYLSMIRSIVAPTLVVQGVSDHIVSPGAVDWMCAVRPDWEQVRMEDTGHTPQMDAPLR
                     FLAVVEDWLARTGPKVAEGDPAPVA"
     gene            1563..2966
                     /locus_tag="VLS86_08760"
     CDS             1563..2966
                     /locus_tag="VLS86_08760"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSO50626.1"
                     /translation="MKLLLISTYELGRQPVHLASPAAALAANGIDVITLDLAVEDWNE
                     GLLDDVAGVAFSVPMHTAMRLANAMSETVRRLRPQLPIAFYGLYAAVGSRQTMADALF
                     VGEYEPDLIAWARALDGSLMRDGGQIDRGVGRSRFYTPNRSMLPSHDNYARLEWEGVA
                     RVAGAVEASHGCRHRCRHCPIPVVYDGRMRVVGSEAVLADIDQLVAAGVEHVTFGDPD
                     FLNAPRYSLDLLRAASAAHPDLTFDVTVKVEHILAHESAWQEMASLGVLFVVSAFEST
                     DRRTLEILDKNHTAEDMGAAVSILRSAGVHIRPTWLPFLPWTSPSDLADLVAFLDSFS
                     LWSATDPVQLGIKLLIPEGSLLETHPDVVPHLVAYEPPSLTWSWEFEHPETDRLQREL
                     DLIAAEGSDCGAEVEDTLAIMRDRIGRSVGVDLQPMPISAATPRLSESWFCCAEPTAA
                     QAAAVGLTIGRVPLASR"
     gene            2977..>3586
                     /locus_tag="VLS86_08765"
     CDS             2977..>3586
                     /locus_tag="VLS86_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50627.1"
                     /translation="MAAVLTADGLIKSFGDLTAVRGVSFAIEEGETFGLLGPNGAGKT
                     TSISMVAGLLEPDGGQIHVSGTPIRTNSTDGKASIGLVPQELAIYPDLTAAENLSFFG
                     RLYGMSGDVLSTLVDEVLEVIGLSDRRADLTKEFSGGMKRRLNIGIGLLHRPLLLILD
                     EPTVGVDPQSRNAILESVEDLSGQGMAVLYTTHYMEEAERLCD"
BASE COUNT          598 a         1151 c         1197 g          640 t
ORIGIN      
        1 agtcgggcat caacctcgga tgggggtagg aggagagccc gtcaccgtcg atctcgaatc
       61 ggaaattgtc caggtgcttc tcgtcgaagc gaccttccag gtaggagcgg gcgtagatgc
      121 cgggcgcagc gtgtccctgc atgtagacgt ggtccccgcc tccgggcgcg tccttgcctc
      181 tgaagaagtg gttgaacccg acctcgtaga gggttgccga tgaggcaaat gtggagagat
      241 ggcccccgat tcccttagac cgatgattgg ccttgatcac catcacggcc gcgttccaac
      301 ggatgtagcg tcgaatccgc cgctcgatgt cctcgtcgcc gggaaactcc ggttcctctt
      361 cgggcgggat ggtgttgatg tacggagtgg tgatcacacc cgccatcccg acgttggtct
      421 cgtgggcgcg ctcgatcagc ttggccacca gaaacctggc ccggtccggc ccttgccccg
      481 cgaccaccgc gtcgagcgag tcgatccact cgctggtctc gacagggtcc gagtcgggaa
      541 gctgattgac gtattcgtcg ccgagagtcc ggccaagatc gcctggcata tggggagagc
      601 ctagttacga gcccggttat gccaccgggg ccgggtcacc ctcggcgacc tttggcccgg
      661 tacgagccag ccagtcctcg accaccgcca ggaagcgaag cggggcgtcc atctgtgggg
      721 tgtggccggt gtcctccatg cggacctgct cccaatcggg gcggacggca cacatccagt
      781 cgactgcgcc cggggagacg atgtgatccg agaccccctg gacgaccaga gtcggggcca
      841 cgatcgatcg gatcatgctc agataggcgg tgcgacggag atagagacgt gcgatcgagg
      901 tcgcagtgcg ggccgtggca tacccggccc acggaagctg cttgcgaatg tgagccatgt
      961 cgcgggagtc cgcgatcagg cccatcggca cccgacccgg cttgtgggtg atcatcgcca
     1021 ggctcttctg gaccagttcc tcgggcgtgt tgctgcgaag aaagtgccgt ccgtagacct
     1081 cgccgatgaa gggagtcgcc tggacggaga gccggatcac cgtgggccag tcgagctggg
     1141 ggtcggggaa gaccggtgga gtggccgggg agaccaggat cagcctatgg acaaggtccg
     1201 gcctggccgc cgccaccagt tccgagagca gacccccact cgagttgccg atcagtgtgg
     1261 tgttcgagga gtcgagggtc tccaggtagg cgactatcga gtcgcggtgg gtgttgagcc
     1321 tgaagtttcc tcggggggga gtcaagccga atcccggcag gtctatggcg cggacccttg
     1381 ccgtccgggc gaggccgggg cccaccgcgt tccagttggt ggtggagcca cccaggccat
     1441 gcaccaggac caggtccggc cccgagccgc caaagtcgac ctggtgcatc ggtcccatac
     1501 tggtgagggc atagtcggtc acccaccgag gctacccatg gccggcccct tagactcatc
     1561 tcgtgaagct gcttctgatc tcgacgtatg agctcggacg ccagccggtc catctcgcat
     1621 caccggcagc cgcgctcgcc gccaacggga tcgatgttat cacccttgat ctcgcggtcg
     1681 aggattggaa cgaaggcttg ctcgacgacg tggcgggagt cgccttctcg gtccccatgc
     1741 acaccgcgat gcgtctcgcc aacgccatgt cggagacggt tcgccgtctc cgaccccagc
     1801 tgccgatcgc gttctacggg ctctatgcgg cggtggggag ccggcagacg atggccgatg
     1861 ccctgttcgt cggcgagtac gagcccgacc tcatcgcctg ggcccgtgcc ctggacggca
     1921 gcctgatgcg cgatggggga cagatcgacc gcggtgtagg gcgatcccgg ttctacacgc
     1981 cgaatcggtc gatgctcccc tcgcatgaca actacgcccg gctggagtgg gaaggcgtgg
     2041 cccgggtcgc cggggcggtg gaggcgtccc acggctgccg gcaccggtgc cggcactgcc
     2101 cgatcccggt ggtgtacgac ggacggatgc gtgtcgtcgg gtctgaggcg gttctcgccg
     2161 acatcgacca gttggtcgca gccggcgtgg agcacgtgac cttcggggac cccgacttcc
     2221 tgaacgcccc ccgctactcc ctggacctgt tgcgggcagc ctctgccgcc caccccgacc
     2281 tgacattcga cgtgaccgtc aaggtcgagc acatcctggc ccacgaatcg gcatggcagg
     2341 agatggcgtc actgggagtc ctgttcgtgg tcagcgcctt cgagagcacc gaccggcgga
     2401 ccctggagat cctggacaag aaccacacag cggaggacat gggcgccgcg gtgtctatcc
     2461 tgaggtcggc gggagtgcac atacggccca cctggctccc cttccttccc tggacgtcgc
     2521 cttccgacct tgccgacctc gtcgccttcc tcgacagctt cagtctctgg tcggccaccg
     2581 acccggtgca gttgggcata aagctcctga tccccgaagg ctctcttctc gagactcacc
     2641 ccgatgtcgt tccccacctc gtcgcctacg agccgccctc gctcacctgg tcgtgggagt
     2701 tcgagcaccc ggagacagac cgacttcagc gagagttgga tctcatcgcc gcggaaggct
     2761 ccgattgcgg cgccgaggtc gaggacacgc tggcgatcat gcgagatcgg atcgggcggt
     2821 cggttggcgt cgatctccaa ccgatgccga tctcggctgc gaccccccgt ctctccgaga
     2881 gctggttctg ctgtgcagag cccacggccg cccaagccgc ggcggtgggt ctgaccatcg
     2941 gtagggtgcc actcgcatcg aggtagaagg gaagtcgtgg cagcggttct gacggcggac
     3001 ggattgatca agagctttgg cgacctcacc gcggtcaggg gtgtcagctt tgccatcgag
     3061 gagggtgaga ctttcggcct cctcggtccc aacggcgcgg gaaagacgac ctcgatctcg
     3121 atggtggccg ggctgctcga gccggacggt ggtcagatcc acgtctcggg aacgccgatc
     3181 cgcacgaatt cgaccgatgg caaggcgtcg atcggtctcg tcccacagga gctggcgatc
     3241 tacccggatc tgaccgccgc cgagaacctc agcttcttcg ggcggctgta cggcatgtcc
     3301 ggggacgtgc tctccacgct ggtcgacgag gtgctggagg tgatcgggct gagcgatcga
     3361 cgcgccgatc tgaccaagga gttctccggg gggatgaagc gacgtctcaa catcgggatc
     3421 gggctcctcc atcgcccact gctcttgata ctggacgagc ccacggttgg cgtcgaccca
     3481 cagtcccgaa acgccatcct cgaatcggtc gaggacctct ccggccaggg aatggctgtg
     3541 ttgtacacca cgcactacat ggaggaggca gagcgtctct gcgatc
//
LOCUS       DATITW010000628         1979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_671316, whole genome shotgun sequence.
ACCESSION   DATITW010000628 DATITW010000000
VERSION     DATITW010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671316"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VLS86_08770"
     CDS             <1..1125
                     /locus_tag="VLS86_08770"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HSO50628.1"
                     /translation="EAVQDALVEALRSRPVRGIPDTPAGWITTVARRRGIDRLRRQTA
                     LARKSVILAGLEMAEAAAKAEIVTSASVEDDRVRMLFACCHPALSVDKQIALTLRTVG
                     GLTTTEIAHAFLVTEQTMAQRLVRAKAKVRDAGIPFKVPEGHELVDRLSAVLAVIYLI
                     FSEGYFATSGQALVRSDLVDSSIGLGRVVATLMPDEPEVLGLLALMLLQDSRRRARAD
                     ASGDLILLQDQDRTLWDREAIDEGMALLERSARLGRPGLYQLQAAIASEHATVETPEE
                     TDWARIVTLYDRLLDIHPAPVARLNRAVAVAESSGPEAGLAALEEVQGELSGYHGFHI
                     ARSEMLRQTGDPKAANQELEVALSLTTNDAERRFLEGKLQ"
     gene            complement(1130..1696)
                     /locus_tag="VLS86_08775"
     CDS             complement(1130..1696)
                     /locus_tag="VLS86_08775"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HSO50629.1"
                     /translation="MSLLGAVLAGGRSSRMGMDKATIEISGSSLLDRVAGALGEVASQ
                     VVVLGGEREGYESWPDQTAIPGPLAGVATALMRMTQDRALVVAVDNAFVSADTLLHLS
                     ALESDLPVVPVDLAGVRQVTCAIYPKGIAEIAWEEAEGGGSIQTLLDRVSFHPVTPDL
                     WSGWGEDGRSWFSVDVPGDIETGLLRFA"
     gene            complement(1742..>1979)
                     /locus_tag="VLS86_08780"
     CDS             complement(1742..>1979)
                     /locus_tag="VLS86_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HSO50630.1"
                     /translation="GHVLRGGTPSPADRLLATRLGTAAASYVAEGIHGVMVAARGEST
                     EPVPLKEIAGITKSVPLDHPWIRSARHLGIAMGD"
BASE COUNT          336 a          669 c          668 g          306 t
ORIGIN      
        1 gaggcggtgc aggacgccct ggtagaggcg cttcgctcga ggccggtgcg cggcatcccc
       61 gacacccctg cagggtggat caccaccgtc gcccgtcgcc ggggcatcga tcgtctccgc
      121 cgccagacgg cgctagcccg gaagagtgtg atcctcgccg gtctggaaat ggccgaggcc
      181 gcggccaagg cggagatcgt gactagcgcc tcggtggaag acgaccgggt ccggatgctg
      241 ttcgcctgct gtcatccggc gctctccgtc gacaagcaga tcgctctcac cctgcgcacg
      301 gtcgggggcc tgaccaccac cgagatcgcc catgccttcc tggtgaccga gcagaccatg
      361 gcgcagcgac tggttcgagc caaggccaag gtccgcgacg caggcatccc gttcaaggtc
      421 ccggaaggcc atgagctggt cgatcggttg tcggccgtcc tcgccgtcat ctatctcatc
      481 ttcagcgagg gttacttcgc cacctcaggg caagctctgg tccggtcaga cctcgtcgac
      541 tcgtctatcg gtctggggcg ggtggtcgcc acgctgatgc cagacgagcc cgaggtcctc
      601 ggcctgctgg cgctgatgtt gctccaggac agccgaagac gagcccgtgc cgatgcatcg
      661 ggggacctga tcctgctcca ggaccaggac cgcacgctct gggatcgtga ggccatcgac
      721 gagggtatgg cgctgctgga gagatcggcc aggttgggac gacccgggct ctaccagcta
      781 caggcggcga tcgcctccga gcacgccacc gtggaaacgc cagaggagac cgattgggcg
      841 cgcatcgtca ctctgtacga ccggctcctc gatatccacc cggcgcccgt ggcccgtctc
      901 aaccgagcag tggccgttgc ggaatcctcg ggaccggagg cgggtctggc cgctctcgag
      961 gaggtgcagg gtgagctctc cgggtatcac ggcttccaca ttgctcggag tgagatgttg
     1021 cgtcagaccg gcgacccgaa agcggccaac caggagttgg aggtggcgct cagcctgacc
     1081 accaacgatg ccgagcgtcg cttcctggag ggaaagctgc agtgaccggt caggcgaagc
     1141 ggagcagccc ggtctcgatg tctcccggga catcgacgga gaaccagctc cgaccgtcct
     1201 ccccccaacc cgaccacagg tcgggagtca ccgggtggaa cgagacccga tcgaggagcg
     1261 tctggatcga ccctccaccc tccgcctcct cccaggcgat ctcggcgatc ccctttgggt
     1321 agatcgcgca ggtgacctgg cgcacgccgg cgaggtcgac gggaacgacg ggcagatcgg
     1381 attcgagggc cgacaggtgc agcagggtgt cggcactgac gaacgcattg tccacggcca
     1441 cgacgagagc tcgatcctgg gtcatccgca tcagggcggt cgccaccccg gccaaggggc
     1501 cggggatcgc ggtctgatcg ggccaggact cgtatccctc ccgttcccca ccgagcacca
     1561 cgacttggga ggcgacctcg cccaacgccc cggccacgcg gtcgaggagc gaactcccgg
     1621 agatctcgat cgtggccttg tccattccca tcctggagct tcggccaccg gccagtaccg
     1681 caccgagcag cgacatgggg ctcaggctag gtgggagacg aaggggcggg gcggggagcc
     1741 atcagtcacc catcgcgatc ccgagatgtc tcgccgatcg gatccatggg tgatcgagag
     1801 gaacgctctt ggtgatcccg gcgatctcct tgaggggcac cggttcggtc gactcaccac
     1861 gggccgccac catcaccccg tggatcccct cggcgacata tgaggcggcc gcggtgccga
     1921 gacgggtggc caggagccgg tccgcaggcg acggcgttcc tccacgcagc acgtggccg
//
LOCUS       DATITW010000629         1655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_712064, whole genome shotgun sequence.
ACCESSION   DATITW010000629 DATITW010000000
VERSION     DATITW010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712064"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1548
                     /locus_tag="VLS86_08785"
     CDS             97..1548
                     /locus_tag="VLS86_08785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSO50631.1"
                     /translation="MIIDILRWVGYSTLIYFIFMQSYMIWLGLRSAAVMRRVHHLDRF
                     GRVSDMLSSQTSPPVSIVIPAFNEAAGIVDSVRSMSIVGYPRFEIVIANDGSTDETLQ
                     VLIDAFHLEKVRIPYRPDIPTANVRGIYRGHGSVDITVIDKENAGRADALNAGINAAR
                     YPYALCTDADVILDADCLVGAMRRVVEDRQRTIAVGGNIRPLNGSRVQLGHLIKAEVP
                     KKLVARMQVLEYVRTFLASRPAWSGMEALPIVSGAFGVWKRSAVIAVGGFTSGHMGED
                     MDLTMRLHRYHIDNAIPYRIVYEPSAVIWTEVPETMRVLKRQRIRWHRGLMTAVKDFL
                     PMTFNPKYGKVGMVTWAAMFLFEYLAPIIEVLGWITIPVAFYLGALNTTSLVILIALA
                     FGVGLVNSLVALLLDEAYGYFNSKADTARLIVMVLIENFGMRQITVAWRIRAIIGGKA
                     TRAWGNMERKGVANLGGPPSVDKTRTSASAAHM"
BASE COUNT          292 a          519 c          536 g          308 t
ORIGIN      
        1 cctcgccgag gcgagacatc gagtcgagct cgacgaagcc agcgacgccg accaggcctt
       61 gatcaggtac gtgagtgccc aggaggtggt gagcgcatga tcatcgacat cctgcgctgg
      121 gtcggctact cgacgctgat ctacttcatc ttcatgcagt cctacatgat ctggctgggt
      181 ttgcggtcgg ctgcggtgat gcggcgggtg catcatctcg accggtttgg ccgtgtctcg
      241 gacatgttgt ccagtcagac atctccgccg gtctccatcg tcatcccggc gttcaacgag
      301 gcggccggca tcgtcgactc ggttcgctcg atgtcgatcg tcggctatcc gagattcgag
      361 atcgtgatcg ccaacgacgg atccacggac gagacgcttc aggtgctcat cgacgctttc
      421 cacctggaga aggtccgcat cccctatcgc cccgacatcc ccaccgccaa cgtgaggggg
      481 atataccggg gccacggcag tgtcgacata accgtgatcg acaaggagaa cgcgggccgg
      541 gcggacgcgc tcaacgccgg gatcaacgcg gcccggtatc catacgccct ctgcaccgac
      601 gccgacgtga tcctcgacgc ggactgcctg gtgggcgcga tgcgacgggt ggtggaggac
      661 cgccagcgga cgatcgccgt tggggggaac atccgccccc tcaatgggag ccgggtccag
      721 ctcgggcacc tgatcaaggc cgaggtgccg aagaagctcg tggcccggat gcaggtcctc
      781 gagtacgttc gcacctttct cgcatcgcgg ccggcgtggt cgggcatgga ggccctgcca
      841 atcgtctccg gtgcgttcgg ggtgtggaag cggtccgcgg tcatcgccgt cgggggtttc
      901 acctcagggc acatgggcga ggacatggac ctcaccatgc gcctccatcg ctatcacatc
      961 gacaacgcca tcccctatcg gatcgtgtac gagccttcgg ccgtgatctg gaccgaggtg
     1021 cccgagacga tgcgggtcct gaagcgtcag cggattcgct ggcatcgcgg gctgatgacc
     1081 gcggtcaagg acttcctccc catgaccttc aacccgaagt acgggaaggt ggggatggtc
     1141 acctgggcgg cgatgttcct cttcgagtat ctggcgccca tcatcgaagt cctggggtgg
     1201 atcaccatcc ccgttgcctt ctacctcggt gccctgaaca cgacttcgct ggtgatcctg
     1261 atcgcgctcg cctttggagt gggcctggtc aactcgctgg tcgcgctgct cctcgacgag
     1321 gcgtacggct acttcaactc caaggccgac accgcccggc tgatcgtgat ggtcctcatc
     1381 gagaactttg ggatgagaca gatcacggtg gcgtggcgga tccgggccat catcgggggc
     1441 aaggccaccc gagcctgggg caacatggag cgcaaggggg tggccaacct cggcggcccg
     1501 ccctcggtgg acaagacccg aacctcagct tctgcggccc atatgtgaaa caaactgtct
     1561 caggttggga cgcagatcgg cgccgattga gtcacaacgg ggccgaaatg cccgaccggg
     1621 gcggagaaat cggtctctga agctgagtca gtttg
//
LOCUS       DATITW010000630         1579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_190749, whole genome shotgun sequence.
ACCESSION   DATITW010000630 DATITW010000000
VERSION     DATITW010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190749"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1127)
                     /locus_tag="VLS86_08790"
     CDS             complement(60..1127)
                     /locus_tag="VLS86_08790"
                     /EC_number="1.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018506 - maleylacetate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetate reductase"
                     /protein_id="HSO50632.1"
                     /translation="MKPFVYEALPGRVIFGDGSLKKVPQELDSLEAGTVMLIASGSSK
                     PLADSLADLMGDRVVARIDQVNPHVPIEDVEEARRLAVDSSTEGVVTIGGGSTIGLGK
                     NIALEAPVRHLTVPITYSGSEMTPIHGVTINGFKRTGRDSKAKPHTVVYDPELGRGLA
                     LAVTAGSVMNAMAHCVEALYAERRNPVTSSMAREGLTALHSGLVRVVSDGFDREGRAS
                     LLFGAFLAGTALGSVGMAIHHKICHVLGGTFGLAHGDANAVVLPHVVAANAVAEPEAV
                     ADIAGVLGVTDPAAGLFELAASAGAPSSLAQLGMKKVDLDRAAAIVIEHPGFNPRPVD
                     GIWIRELLEEAFEGKKPTATA"
     gene            complement(1124..>1579)
                     /locus_tag="VLS86_08795"
     CDS             complement(1124..>1579)
                     /locus_tag="VLS86_08795"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008483110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HSO50633.1"
                     /translation="QIDRFGNLNSTVIGAYEHPTVRLPGAGGAPEIAAAAGEVFVMLR
                     HQKRMFVEAVDFVTSVGFGRDGKGRPGCWGAGPTAVITDLGVLRPDPVTHELILTAVH
                     PGVEVDRVVDETGWDLGVSPVVEVTDPPTAEELAVLRDLMERTEAAHRR"
BASE COUNT          289 a          523 c          486 g          281 t
ORIGIN      
        1 ccagttgcgt cgactcggcc cgcgcccgcc ttcgtattcc ttgacccccc atcctcctcc
       61 tatgcggttg ccgtcggttt cttgccttca aaggcttctt ccagaagctc gcggatccag
      121 atgccgtcca cgggccgggg attgaacccc gggtgctcga tcacgatggc ggccgctcga
      181 tcaagatcca ccttcttcat tccgagttga gccaggctcg atggcgcccc cgccgacgcg
      241 gccaattcaa agaggcccgc ggccggatcg gtcacaccga gaacgccggc gatgtcggcg
      301 accgcctctg gttcggcgac ggcattggcc gctaccacat gcgggagcac aacggcgttg
      361 gcgtcaccat gggccagacc gaacgtgcct cccagaacgt gacagatctt gtgatggatt
      421 gccatgccaa ccgagccgag ggcggttccc gccagaaagg caccaaacag caaggaggct
      481 cggccctccc gatcgaagcc atcggaaacc actcgaacca gacccgagtg gagggcggtg
      541 aggccttccc gggccatcga cgaggtgacg ggatttcggc gctcggcgta cagagcctcg
      601 acgcaatggg ccatggcgtt catgacgctt ccggccgtga ccgccaacgc aaggccccgg
      661 cccagttcgg ggtcgtatac caccgtatgc ggcttggctt tcgaatcccg cccggtgcgc
      721 ttgaaaccgt tgatggtcac cccgtgaatc ggggtcatct ctgaacccga gtaggtgatg
      781 ggaacggtga ggtgcctgac cggcgcctcg agggcgatgt tcttgcccag cccgatggtc
      841 gagccacctc cgattgtgac gacgccctcg gtcgaagaat cgaccgcgag tcgacgggcc
      901 tcctcgacat cctcgattgg cacgtgggga ttgacttgat cgattcgcgc cactacccga
      961 tcccccatca gatcagccag cgagtcggcc aggggcttcg atgatccact ggcaatcagc
     1021 atcacggtcc ccgcctcgag ggagtcgagt tcctgaggga cctttttcag cgagccgtcg
     1081 ccaaagatca cccggccggg cagcgcttca taaacgaagg gcttcatcga cgatgagccg
     1141 cctcggtccg ttccatcaga tcacgaagga ccgccagttc ctcggctgtg ggtgggtcgg
     1201 tcacctctac caccggggag acaccgaggt cccagccggt ctcatcgacc actcgatcga
     1261 cttctacgcc gggatggacg gcagtgagga tgagttcgtg agtaactgga tcgggccgga
     1321 gtactccgag atcggtgatg acggcggtcg ggccggctcc ccagcacccc ggccggcctt
     1381 tgccatccct tccaaatccg accgaggtga cgaagtcgac cgcctcgacg aacatccgct
     1441 tctggtgtcg cagcatgacg aacacctcgc cggccgcagc ggcgatttcg ggagcgcccc
     1501 cggcgcctgg gagacgaaca gtggggtgct cgtaggcgcc aatgaccgtg ctgttgagat
     1561 ttccgaaacg atcgatctg
//
LOCUS       DATITW010000631         3016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_190831, whole genome shotgun sequence.
ACCESSION   DATITW010000631 DATITW010000000
VERSION     DATITW010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190831"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VLS86_08800"
     CDS             <1..784
                     /locus_tag="VLS86_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSO50634.1"
                     /translation="GSHWTGVPLASLLEEAGPLEDARFVSFEAYSQRDHHTSLPLEVA
                     VSDCWLVHQFDGSPLEVGHGGPVRSVTTSRYFYKSLKWVKRVVLLAEDRPGWWEQDSF
                     YHNNADPWPGNERFTTGSIRPEQLARFLAADRYDKYRGRVLMGLDLRSWVPASTDLRR
                     LYLKYCDLRGVSLEGVDLRECNLSLSDLRGAQLGGADLSGSDLEGVNFSGADLSGANL
                     SGTALSATRFVDDTGAATLAGANFEGSFGLVEEQEEFVSRHT"
     gene            836..1816
                     /gene="trpS"
                     /locus_tag="VLS86_08805"
     CDS             836..1816
                     /gene="trpS"
                     /locus_tag="VLS86_08805"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HSO50635.1"
                     /translation="MTSRKRIFSGIQPTGDFHVGNYVGALKNWVALQEEYETIYCVVD
                     LHAMTVPIEPAELRAGRLETAKMLLAVGIDPERSLLYHQSEVPQHVELSWILGTMTGL
                     GQLERMTQFKEKADRARQNLGIFAYPVLQAADILVHKVHAVPVGDDQSQHLELTRDLA
                     QRFNARFGEEFPLPETITPEIGARVMSLTDPSSKMSKSDPSPRSRIVLKDTPDDVMKK
                     VKSAVTDAGAGVDHDWVSKPGISNLIELFSVFADRSIADVVAEHRDTGYGAFKIVVAE
                     AIIEGLAPIRARYDSLDDFDVEKIMARGAATARDRAEISMKSVREKVGIG"
     gene            complement(1813..2517)
                     /locus_tag="VLS86_08810"
     CDS             complement(1813..2517)
                     /locus_tag="VLS86_08810"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HSO50636.1"
                     /translation="MPRPWSLLDFFLIVLGGLLGAGVFVAFSALIGDEEVFLVLALAG
                     QYVGHLLVLWLIARNKGNPDLGFNVEGRDIVYLGLGLFLQLGLAVLFLPLTTLLLPDG
                     DSAQQVGTALSGLETTAARVAAMVVAVVVAPVTEELIFRGVLLKSQERRSPRTIMVIT
                     ALVFSVFHLLGLDPERMLSAAAVVLPQLFIVGVVLAWVTLRSKRLGPAIFIHSGFNLL
                     AAIVLLLPPELLESVG"
     gene            2592..>3016
                     /locus_tag="VLS86_08815"
     CDS             2592..>3016
                     /locus_tag="VLS86_08815"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004984376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HSO50637.1"
                     /translation="MEIDIGIGKSGRQAYGFDDIAIVPSRRTRDPDDVDISWQLDAFK
                     FELPMMGSAMDSAISPATAVKIGQLGGLAVLNLEGLWTRYEDPEPYFEEVAELSAEKA
                     TLRMQEIYLEPVMPDLIRQRVAEITAQGLVTAGSLTPQPV"
BASE COUNT          577 a          988 c          970 g          481 t
ORIGIN      
        1 cggctcgcac tggaccggtg tgccgctggc ctcgctgctc gaggaggcgg gaccgctcga
       61 agatgcccgt ttcgtctcgt tcgaggccta ctcgcaacgt gaccaccaca cgtccctgcc
      121 gctagaagtg gccgtctccg attgctggct ggtccatcag ttcgacggct cgccgctgga
      181 ggtcggtcat ggcggcccgg tgcgatcggt gacgacgagc cggtacttct acaagtcgct
      241 gaaatgggtc aagcgcgtcg tgctcctcgc cgaggatcga cccggctggt gggagcagga
      301 ttcgttctat cacaacaatg cagacccctg gccgggcaat gagcgcttca ccaccggctc
      361 gattcgcccc gagcagctgg cccggttcct cgccgccgat cgctacgaca aataccgggg
      421 ccgggtgttg atggggctcg acctcaggtc atgggtcccg gcctccaccg acctgcgacg
      481 gctctacctc aagtactgcg atctgcgtgg cgtgtcactg gagggggtcg acctgcgtga
      541 gtgcaatctc tcgctgagcg acctgcgcgg ggcccaactc ggcggagccg atctctcggg
      601 ctcggatctc gagggagtga acttctcagg agcggatctc tcgggggcga atctctccgg
      661 caccgcactc tccgccaccc gcttcgtcga cgacacgggg gcggcgaccc tggccggcgc
      721 caacttcgaa ggatccttcg gtctggtcga ggagcaggaa gagttcgtct cacgccatac
      781 ctagtctggc ccccgcaggt ggccggtgtc ggcgaagtag agggatgaat cgatcgtgac
      841 cagtcggaag aggatcttct cgggcatcca acccacgggc gactttcacg tcggcaacta
      901 cgtgggagcg ctcaagaact gggtcgccct ccaggaggaa tacgagacca tctattgcgt
      961 cgtcgacctg cacgcgatga ccgtgcccat cgagccggcc gagctgcggg ccggtcgttt
     1021 ggagaccgcc aagatgctgc tcgcggtcgg gatcgacccc gagcgctcac tcctctatca
     1081 ccagtccgag gttccccagc acgtcgagct ctcctggatc ctgggcacca tgaccggcct
     1141 cggccagctg gagcgcatga cccaattcaa ggagaaagcc gatcgcgccc ggcagaacct
     1201 gggcatcttc gcctacccgg tgctgcaggc cgccgacatc ctcgtccaca aggttcacgc
     1261 ggtcccggtc ggagacgatc aatcccagca tctggagctg accagagacc tggctcagcg
     1321 attcaatgcg cgattcggcg aggagttccc actcccggag accatcactc ccgagatcgg
     1381 ggccagggtc atgtccctga ccgacccgtc atccaagatg tccaaatccg atccgagccc
     1441 tcgctcccgg atcgtgctca aggacacccc tgacgatgtg atgaagaagg tgaagtcggc
     1501 ggtcaccgac gccggggccg gggtcgacca cgactgggtc tccaagcccg ggatctccaa
     1561 cctcatcgag ctgttcagtg tctttgccga ccgctccatc gccgacgtcg tcgccgagca
     1621 ccgggacacc ggctacggcg cgttcaagat cgtggtggca gaggccatca tcgagggatt
     1681 ggccccgatc cgagccaggt acgactcact ggacgacttc gatgtggaga agatcatggc
     1741 caggggcgcc gccacggcac gggaccgcgc cgagatctcg atgaagagcg tgcgggagaa
     1801 ggtcgggatc ggctagccga ccgattcgag cagctcgggt ggcagcagca ggacgatggc
     1861 cgccagcagg ttgaaccccg aatggatgaa gatcgccggg ccgagccgct tgcttcgcaa
     1921 cgtcacccag gccaggacca cgccgacgat gaagagttgg ggcagcacca ccgcggccgc
     1981 ggagagcatc cgctccgggt ccaggcccag caaatggaag acggagaaga ccagcgcagt
     2041 gatcaccatg atcgttcgcg ggcttcgacg ctcctggctc ttgagcaaca ccccgcggaa
     2101 gatcagctcc tcggtgacgg gagcgaccac caccgcgacg accatggccg caacccgggc
     2161 cgcggtcgtc tccagcccgg acaacgccgt gcccacctgt tgcgccgagt cgccgtcggg
     2221 gaggagcagg gtggtgagcg ggagaaacag gaccgccaac ccaagctgga gaaacagccc
     2281 caatccgaga tagacgatgt ctcgtccctc gacgttgaac ccgaggtccg ggttgccctt
     2341 gttgcgggcg atcagccaca ggaccagaag atgaccgaca tactggccgg cgagggcgag
     2401 gaccagaaac acctcttcgt cgccgatcag ggcgctgaac gccacgaaca cgcccgcccc
     2461 gagcaatccg ccgaggacga tgaggaagaa gtcgagcagc gaccatggtc tcggcacggc
     2521 gcggcagcct actgatggcc gggggtgatc caatgttacg ctggaacgag cgtccccgga
     2581 ggtgagcgga agtggaaatc gacatcggaa tcgggaagtc cggccgccag gcgtacggct
     2641 tcgacgacat agcaatcgtc ccctcgcgac gcacccgcga tcccgacgac gtcgatatct
     2701 cctggcagct cgacgcattc aagttcgagt tgccgatgat gggctcggcc atggactctg
     2761 cgatcagccc ggcgacggcg gtcaagatcg gccaactcgg gggactggcc gttctcaatc
     2821 tcgaaggtct gtggacccgc tacgaagacc ccgagcccta tttcgaggag gtcgccgagc
     2881 tgtcggcgga aaaggccacc ctgcgcatgc aggagatcta cctggagccg gtgatgcccg
     2941 accttatccg ccaacgtgtg gccgagatca cggcccaggg gttggtgacc gccggaagtc
     3001 tcaccccgca gccggt
//
LOCUS       DATITW010000632         1698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_712209, whole genome shotgun sequence.
ACCESSION   DATITW010000632 DATITW010000000
VERSION     DATITW010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712209"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1698
                     /locus_tag="VLS86_08820"
     CDS             <1..>1698
                     /locus_tag="VLS86_08820"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HSO50638.1"
                     /translation="EGAEDLAVSAEALLATVRGHLHDYQTASDAASQELLSNRLSALA
                     DATAGLAAEMDFSFLMNSDNKLLTVGYRVGENALDQSHYDLLASEARLASFVGIAKGD
                     VPPSHWFRLGRSMTPMGVESALISWSGSMFEYLMPLLVMRSPPESILDTTYRAVVRRQ
                     IEYGNERQIPWGISEAAYAARDIHLVYQYRAFGVPGLGFERGLSEDLVVAPYATLLAT
                     MVEPRDAVRNLIRLNSLGALGRYGYYEALDFTPSRLPEDGKSVLVRAYMAHHQAMSLI
                     SLTNVVFAGLCRDLFHSDPLVRAAELLLHERVPRDVEVARPRAEEVAESAHPLEEAKG
                     VVRRISSPHGPGLVTHALSNGRLSTVITAAGSGYSQWQGLAVNRWREDATRDNFGSYF
                     FIRDGAGRTWSAGYQPTLAEPDSYQVAFSEDKAEISRRDGNVSTRLTVVVSEEDDAEV
                     RRVSLTNLGPVAREVEVTSYAEIVLASARADAAHPAFSNLFVQTEVVAGRDALLASRR
                     PRSAEEETVWAGHVLAVTGEVVGGIQYATDRARFLGRGNQIHRAAAVDRPLSNTAGRV
                     "
BASE COUNT          277 a          547 c          561 g          313 t
ORIGIN      
        1 ggagggtgcc gaggatctgg cggtctcggc cgaagcgctg ttggcgacgg tgcggggcca
       61 ccttcacgac tatcagacag ccagcgatgc ggccagccag gagcttctct ccaatcgtct
      121 ctccgccctc gccgatgcga ccgccgggct ggcggctgag atggatttct cgtttctgat
      181 gaactccgac aacaagctgc tgacagtcgg ctatcgcgtc ggggagaacg ccctcgacca
      241 aagccactac gacctgctgg cctcggaggc ccgtctggcc agcttcgtcg gcatcgccaa
      301 aggtgacgtt ccccccagcc attggttccg tctgggccgg tcgatgactc caatgggtgt
      361 cgagtccgct ctgatctcgt ggtcgggctc gatgttcgaa tacttgatgc ccctgttggt
      421 gatgcgctcg ccacccgaga gcatcctcga caccacctac cgggcggtcg tgcgacgcca
      481 gatcgagtac gggaatgagc gccagatacc ttgggggatc tccgaggcgg cctacgccgc
      541 tcgcgacatc cacctcgtct atcaataccg ggcatttggg gtcccggggc tcggcttcga
      601 acggggtctc agcgaggacc tggtggtggc tccgtatgca accttgctgg cgaccatggt
      661 cgagccgcgc gacgcggtga ggaacctgat ccggctcaac agtctggggg cgctgggcag
      721 atacgggtac tacgaggcgc tcgacttcac cccgagccgg ttgcccgagg acgggaagtc
      781 cgtcctggtc cgagcctata tggcccacca ccaggcgatg tcactgattt ccctgaccaa
      841 cgtcgtcttt gccgggcttt gcagagacct gtttcattcc gacccgttgg tccgggcggc
      901 cgagctgttg ttgcatgaga gggttcctcg cgatgtggag gtggcccgtc cccgtgccga
      961 ggaggtcgcc gagtcggctc atcccctgga agaggcgaag ggggtggtcc gtcggatctc
     1021 gtcgccacac gggccgggcc tggtcactca tgccctgtcc aacggccgtc tcagcaccgt
     1081 catcaccgcc gctggttccg gttacagcca gtggcagggg ctggcggtca accgctggcg
     1141 ggaagacgcc actcgcgaca acttcggcag ctacttcttc atcagggacg gggcggggcg
     1201 cacctggtcg gccgggtatc aaccgactct ggccgagccc gactcctacc aggtggcctt
     1261 cagcgaagac aaggcagaga tctcgcgtcg tgacggcaac gtctccacca ggctgacggt
     1321 ggtggtgtcc gaagaggacg acgccgaggt ccgtcgggtg agcctcacaa atctgggccc
     1381 ggtggccaga gaagtggagg tcacctcata cgccgagatt gtcctggcat ccgcccgggc
     1441 cgatgccgct caccctgctt tctccaatct ctttgtgcag accgaggtgg tggccggacg
     1501 tgacgctctg ctcgccagcc gccgaccacg ctcggccgaa gaggagacgg tgtgggcagg
     1561 tcatgtcctc gcggtcacag gcgaggtagt cggtgggatt caatacgcaa cggatcgggc
     1621 gcggttcctc ggccgaggaa accagatcca tcgcgccgcc gccgtcgatc gtcccctgtc
     1681 gaacaccgcg ggccgggt
//
LOCUS       DATITW010000633         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_391373, whole genome shotgun sequence.
ACCESSION   DATITW010000633 DATITW010000000
VERSION     DATITW010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391373"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            375..>1525
                     /locus_tag="VLS86_08825"
     CDS             375..>1525
                     /locus_tag="VLS86_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50639.1"
                     /translation="MNNYAGLWLATALAAVLAYRRVMVLIAVVVALFALPASEAQAAP
                     DTECRGTIFAVMIEGKIVVPDGAECELVGTTVDGSIIVGVGSTLIANGIEVLKPGTIP
                     IQASGFTRLEVTGLRKFVGPGDPEELQPSEVLGSIQAVGGGTVIISDTILGGALQISD
                     NSDSVLITDTFAEGAVQLGKNTGEVSVTDSFLGEGVEISETEGDVTFTRNTSDESDLT
                     IGKTNGAVTVEDNPSLGENFEVSETDGELRVLGNFINDNMEISENEGGVIVSGNTIGD
                     NLTVSKITGEVTFPVPCDPLDPECVLGETVIEPASVIIVGNTVGDGVKVLETTGEVIF
                     AENVVEAGAQILQPIGQATANNHHIGFVPVPSGGSLEVTEASAGASVSF"
BASE COUNT          296 a          487 c          477 g          265 t
ORIGIN      
        1 gccaatttcc accaacctcg cgacacgacc cctgcgacat ttgatcggcc attcggtgag
       61 agtcgcgcga cagagggctc agcgcgccct ctgccaccag cggtcatagg cctgcgcgag
      121 cgcgtcgtca cggccgaaca gcggcagctc gtccggaccc gcccgctcca gcaggcgcag
      181 aaaccatgtt cttgcgagac ctcggttcac ttcggccctt gtagcctttc ccgccgcgct
      241 gaaccccgca acattgcctg gaaaatttat tgttgagcta atccaagtta gtgcggacca
      301 tagatcgcta ggcaatcatt ccacgacagt ttgggaatcc tgacgacatt tctgcagctt
      361 ggtgaggacg cgctatgaac aactacgcgg gtctctggct ggcaacggca ctggctgcgg
      421 ttctcgcgta tcgccgagtg atggtcctca tcgctgtggt cgtagcgctc ttcgcccttc
      481 ccgcctcgga ggcgcaagcg gcccccgata ccgaatgccg cggcaccata ttcgccgtta
      541 tgatagaggg caagatcgtg gtgcccgacg gcgcggaatg cgagctcgtc ggcacgaccg
      601 tggacggctc tattatcgtc ggggtcgggt ccaccctgat cgcgaacggg atcgaagtcc
      661 tcaagcccgg gacgatcccc atccaggcgt ccggctttac caggctagag gtcaccgggc
      721 ttcgcaagtt cgtcgggccc ggagaccccg aggagctgca gccctcggaa gtgctcggca
      781 gcattcaagc cgtcggcggt ggtacggtga tcatctccga taccatcctt ggcggcgcgc
      841 tccagatctc ggacaacagc gactccgtgc tcattacgga cacattcgcg gagggcgccg
      901 tgcagctggg caagaacacg ggggaggtgt cggtgacaga cagcttcctc ggcgagggcg
      961 tcgagatctc cgagaccgag ggcgacgtca ccttcacccg caacacctcc gatgagtcgg
     1021 acctgacgat cgggaagacc aacggcgccg tgaccgtgga ggacaatcca agcctcggcg
     1081 agaacttcga ggtctcggag accgacggtg aattgagggt gctcggcaac ttcatcaacg
     1141 acaacatgga gatctccgag aacgagggcg gtgtgatcgt ctccggcaac acgatcggtg
     1201 acaacctgac cgtgtccaag atcacggggg aggtaacgtt ccccgtccca tgcgaccccc
     1261 tggatccgga gtgcgtgctc ggcgagaccg tgatcgagcc ggcgtccgtg atcatcgtcg
     1321 gcaacacggt aggcgacggc gtcaaggtcc tcgagacaac cggcgaggtg atcttcgccg
     1381 agaacgtggt cgaggcaggc gcgcagatcc tacagcccat cggccaggcc acggccaaca
     1441 accaccacat cggcttcgtc ccggtgccgt ccggcggcag cttggaggtg acggaggcct
     1501 ccgcaggggc gagcgtctcg ttcaa
//
LOCUS       DATITW010000634         1805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_431808, whole genome shotgun sequence.
ACCESSION   DATITW010000634 DATITW010000000
VERSION     DATITW010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431808"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="VLS86_08830"
     CDS             complement(<1..1364)
                     /locus_tag="VLS86_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSO50640.1"
                     /translation="MIIVDAALEDRHRSGDPIRVGMVGAGFMGRAIALQIIQATKGIE
                     LVAIANRTLEKASRAYQEAGIEAEVVEDASGLHREIERGKPAITEDPSLLCRAEGIDA
                     IIEVTGTVEFAARVAVEAIDHRKHVVLMNAELDGTLGPTLKERADESGVVITNADGDQ
                     PGVIMNLYRFVRGIGVRPVLCGNIKGLHAPHRTPSTQEGFSRKWGQNPSMVTSFADGT
                     KISFEQAIVANATGMRVARRGMLGPGVEPGTTIQEAVRSYPLEELLDGPGIVDYVVGA
                     VPSPGVFVLGTTDHPMQRQYLNLYKLGEGPLYCFYTPYHLCHFEVPNTVARAVLFGDA
                     TIAADGAPRVDVVTAAKVDLKAGESLDGVGGFKTYGLAENAEVAAAQRLLPKGLAENC
                     IVSRDISRDAVLTYDDVDLPPGRLVDDLRREQEERFALRGATMLSVLSPLYFLREFVE
                     GLAET"
     gene            complement(1391..>1805)
                     /gene="rfbC"
                     /locus_tag="VLS86_08835"
     CDS             complement(1391..>1805)
                     /gene="rfbC"
                     /locus_tag="VLS86_08835"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HSO50641.1"
                     /translation="ETRIAQCNVSFNRHTGTLRGMHLQRPPYGEVKLIRCVRGSLFDV
                     IIDIRPDSVSYKQWAGVELSDRNRKMLYVPPGFAHGFQTLEDDTETFYMVSEFYVPGA
                     EDGLRWDDPMFGIEWPLGEPREISDKDRSWPDFVG"
BASE COUNT          327 a          588 c          539 g          351 t
ORIGIN      
        1 gtctcagcta gcccctcgac gaactcacgc aggaagtaga ggggggacaa cacggacagc
       61 atcgtcgcgc ctcgaagcgc aaaacgctcc tcctgttcgc gccgcagatc gtcgacgagc
      121 ctcccgggcg gtaagtcgac atcgtcgtag gtcagaaccg cgtcgcggga gatgtccctc
      181 gagacgatgc agttctccgc cagccccttc ggcagaagcc gctgcgctgc agctacctcg
      241 gcgttctcgg caagtccgta ggtcttgaag ccgccgacac cgtcgagcga ctcccctgcc
      301 ttgagatcca ccttggcggc ggtgacgacg tcgacacgcg gagctccgtc ggcggcgatc
      361 gtcgcatcgc cgaagagcac cgctcgagcg acggtgttag gtacctcgaa atggcagagg
      421 tgatacggcg tatagaagca atagaggggt ccctcaccga gcttgtacag attcaggtac
      481 tgacgctgca tgggatggtc cgtcgtgcca agcacgaaca cgcctgggct cggcactgca
      541 ccaacgacat agtcgacgat tccgggaccg tcgaggagct cctcgagggg gtacgaccga
      601 acggcctcct ggatcgtcgt ccccggttcg acgccggggc cgagcatccc tctgcgcgcc
      661 actcgcatgc ccgtcgcgtt tgccacgatc gcttgctcga aggagatctt cgtcccgtcg
      721 gcgaacgacg tgaccatgct ggggttctga ccccatttcc gagagaatcc ctcctgcgtg
      781 gacggggttc gatgaggggc atggaggccc ttgatgttgc cgcacaggac gggccggaca
      841 ccgatccccc ggacgaagcg atacagattc atgatgacac caggctgatc cccatcggcg
      901 ttcgttatga cgacgcctga ttcgtccgcg cgttccttga gggtgggccc cagcgtgcca
      961 tcaagctcgg cgttcatcag gacaacgtgt ttgcggtggt cgatggcctc gaccgcaaca
     1021 cgtgcggcga actcgaccgt tccagtgacc tctatgatgg cgtcgatacc ctccgcgcgg
     1081 cagagaagtg aggggtcctc tgtgatcgca ggcttcccac gctcgatctc tcggtggagg
     1141 cctgatgcat cctcgacaac ctcggcctcg attcctgcct cctggtacgc acggctggcc
     1201 ttctcgagcg tcctgtttgc gatggcgacc agctctatcc ctttcgttgc ttggatgatc
     1261 tggagcgcga tggctcgtcc catgaacccg gcacccacca tccccacgcg gatcggatcc
     1321 ccggaccgat gccgatcttc gagtgcagcg tcgacgatga tcacccgcgt ctctcctctc
     1381 atcgctccgg ctagccgaca aagtcgggcc aactccgatc tttgtctgag atctctcttg
     1441 gctcaccgag cggccactcg atcccaaaca tggggtcgtc ccaccttagt ccgtcctcgg
     1501 cgcccgggac gtagaactcc gacaccatgt agaacgtctc cgtgtcgtct tccagtgtct
     1561 gaaaaccgtg tgcgaagcca ggtgggacat acaacatctt gcggttgcgg tcgctgagtt
     1621 ccacacccgc ccactgcttg tacgagacag agtcaggacg tatgtcgatg atgacgtcga
     1681 agagcgagcc tcggacacac cgaatcagct tcacctcccc gtacggcggg cgttggaggt
     1741 gcatgcccct gagcgttccc gtgtgcctgt tgaaggaaac gttgcactgg gcgatgcgcg
     1801 tctcg
//
LOCUS       DATITW010000635         1896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_111643, whole genome shotgun sequence.
ACCESSION   DATITW010000635 DATITW010000000
VERSION     DATITW010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111643"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VLS86_08840"
     CDS             complement(<1..419)
                     /locus_tag="VLS86_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50642.1"
                     /translation="MKRHRVTALIAVLVLVLAACTGDVTETSTTLGEEEPTTTAAEAP
                     STTAGEEPTTTAGGDEDPLAEYGESTDADPALVEKAMGPVADVPEIVLASIARASQEL
                     DQATIDKAIECYTNAVECETGTGGEVIMGYADGGGLNV"
     gene            complement(490..1539)
                     /locus_tag="VLS86_08845"
     CDS             complement(490..1539)
                     /locus_tag="VLS86_08845"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO50643.1"
                     /translation="MTTTKSPSSAVTSFVVASRYMPVYLAIVLLIVVATIAAPATLAE
                     AGLSQISLLGTYLAIAALGQMLVIMTGGIDLSVPGTFLLGGLLMVGVGQQSDDRIWVA
                     YLTAIGIAALIGLVNGILIGGLKLNALIVTLAVGQVVAGIANRYYTTTAIQKPVPEGL
                     TAWATTPFLGITRIFWVGVAVTVVLVIVFRYSGAGRRFQVVGANPVASYIVGLRVNLN
                     QVAAYVVAAVLYATAGVLLAASLRSPGVTVGAPYLLGPIAAVVIGGASLTGGLASPIS
                     TWAAAFFLAGLTQMMRVMGLPTALQFVVFGLVIIGGMLVSGDRIIKAVERVLRDRSKP
                     GRRSEGTLAVGTGET"
     gene            complement(1536..>1896)
                     /locus_tag="VLS86_08850"
     CDS             complement(1536..>1896)
                     /locus_tag="VLS86_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSO50644.1"
                     /translation="GIPTLLNTREVLRYATVEAARVAGVGDVVGSLAPGRQADLILLR
                     TDRANIFPINDPIGAVVWGMDTSNVDWVFVAGRPVKRAGVLQGDVARATDLANAALGR
                     VTAASGLHTGAALEGAR"
BASE COUNT          328 a          641 c          613 g          314 t
ORIGIN      
        1 acgttgagac cgccgccgtc ggcgtatccc atgatgacct caccaccggt gccggtctcg
       61 cactccaccg cattggtgta gcactcgatg gccttgtcga tggttgcctg atcgagttcc
      121 tggctggccc ttgctatgga cgccagcact atctcgggca catcagcgac cgggcccatc
      181 gccttctcca ccagtgccgg gtcggcgtcg gtggactcgc cgtactcagc gagaggatcc
      241 tcgtcacctc cggccgtggt cgtgggctcc tcaccagccg tggtgctggg cgcctcggcg
      301 gcggtcgtgg ttggctcctc ctcgcccagg gtggtactgg tctcggtcac atcgcccgta
      361 caggcggcca ggacgagcac caacacggcg atcagggctg tcaccctgtg tcttttcatc
      421 atcctcctcc attcgttgac tgtgtttcgt ttctcccgct tttgcggttg gtcgtcggtt
      481 ctccttctct cacgtctcgc cggttccgac ggcgagagtc ccttcactgc gccgccccgg
      541 tttcgaccgg tcgcgcaaaa ccctctcgac agccttgatg atccgatccc cggagacgag
      601 catccccccg atgatcacca ggccgaagac aacgaactgc agcgcggttg gcaggcccat
      661 gacccgcatc atctgcgtga ggccggcgag gaagaatgcg gcggcccaag tggaaatcgg
      721 gctggccagg ccgccggtga gcgatgcgcc accgatcacc accgcggcga tagggcccaa
      781 caggtagggg gcgccaacgg tgacccccgg agagcgcagg gaggccgcca acaggactcc
      841 cgcggtggcg tagagcacgg cggccaccac gtaagcggcg acctggttga gattcacccg
      901 aaggccgacg atgtacgagg caaccgggtt cgccccgacc acctggaatc gccttccggc
      961 cccgctgtag cggaacacga tcaccaggac caccgtcacc gccaccccga cccagaagat
     1021 ccgggtgatc ccgaggaatg gggtggtcgc ccacgcggtg aggccttccg gcaccggctt
     1081 ctggatggcg gtggtcgtgt agtagcgatt ggcgatcccg gccacgacct gaccgactgc
     1141 gagggtgacg atgagggcat tgagcttgag cccaccgatc aggatcccgt tcaccaggcc
     1201 gatgagtgcc gcgatgccga tggcggtgag gtaggcgacc catatccggt cgtccgactg
     1261 ctggcccacc ccaaccatca acaacccacc gagaaggaag gtgccaggga cgctgaggtc
     1321 gatcccaccg gtcatgatca ccagcatctg acccagcgcc gcgatcgcga gataagtgcc
     1381 gaggagggag atctggctga gcccggcttc ggccagtgtc gccggggccg caatcgtggc
     1441 gaccacgatc aggaggacga tcgccaggta gaccggcatg tagcgggagg ccacgacgaa
     1501 ggaagtgact gccgaggaag ggctcttggt ggttgtcacc tcgccccttc cagagcggcg
     1561 ccggtgtgga gcccggaggc tgctgtcacg cgccccaggg ccgcgttggc gagatcagtc
     1621 gcccgcgcca cgtcaccctg gagcactccg gctcgcttca cagggcgacc ggcgacgaaa
     1681 acccagtcga cgttggaggt gtccatcccc cacaccaccg cgccgatggg gtcgttgatg
     1741 gggaagatgt tggcccggtc ggtgcgaagc aggatgagat cagcctgtcg ccctggtgcc
     1801 agtgacccca ccacgtcacc gactccggcc acacgagccg cctcgaccgt cgcgtagcgg
     1861 agcacctccc gggtgttgag cagggtcgga atgcct
//
LOCUS       DATITW010000636         1599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_671628, whole genome shotgun sequence.
ACCESSION   DATITW010000636 DATITW010000000
VERSION     DATITW010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671628"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VLS86_08855"
     CDS             complement(<1..206)
                     /locus_tag="VLS86_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50645.1"
                     /translation="MTRKQSTPDELLDAPEGAPVLYLLDAGTDHEAGMMQRWLSDQLG
                     SDADTLRISSSRRGRGGDTEELARV"
     gene            242..697
                     /gene="dtd"
                     /locus_tag="VLS86_08860"
     CDS             242..697
                     /gene="dtd"
                     /locus_tag="VLS86_08860"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HSO50646.1"
                     /translation="MRIVLQRVSRASVTVAGEVVGSIADGLLLLVGIEPTDTPQDVDR
                     AADKVSNLRIFADEGGLMNRSIIDIGGSALVVSQFTLISDMRRGRRPSFVGAADPGTA
                     APLVERLAEMLRDAGIHTETGVFGASMAVDLVNDGPVTVILEIREGRVS"
     gene            complement(690..1274)
                     /locus_tag="VLS86_08865"
     CDS             complement(690..1274)
                     /locus_tag="VLS86_08865"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HSO50647.1"
                     /translation="MIALALILGYLVGSIPTSVWLGRLWGVDLRADGTTNPGANNARA
                     LGGYPLAALVLVMELVKGLAAVAVGLAIGDDPGAVAAGLGAIAGNVYNVWFRFEGGKG
                     LSITGGVLLGIWPVIFPVVVVVIIVSWALTKSSGRATLATLVVLIASALAWDQVGITY
                     AWGVEEPRLLLIAAVGITLLLFPKHFYDARHPIN"
     gene            complement(1271..>1599)
                     /locus_tag="VLS86_08870"
     CDS             complement(1271..>1599)
                     /locus_tag="VLS86_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HSO50648.1"
                     /translation="IVDFKSGRPRDDPSLIVQLQAYAMAATKVDFGVDKPEQIDVSFA
                     YLGGGLAVETERADDTWRGRAEQSLTRLTDAIEDSRFEPTPGEWCRRCDFLRFCEAGQ
                     QWVADG"
BASE COUNT          270 a          520 c          526 g          283 t
ORIGIN      
        1 actcgggcca gctcctcggt gtccccgcct ctcccgcgac gcgacgagga gatgcgcagc
       61 gtgtcagcat cgcttccgag ctgatcgctg agccaccgct gcatcatgcc ggcctcgtgg
      121 tcggtccccg cgtcgagaag gtagagaacc ggcgctccct caggggcatc gagtagctcg
      181 tccggggtcg attgcttcct ggtcacgatc ccactctatt tggctgccac aatcggtccc
      241 cgtgcggatt gtgctccagc gcgtgtcgcg agcctcggtg accgtggcgg gcgaagtggt
      301 cggatcgatc gccgatggcc tcctcctcct ggtcgggatc gagccgaccg acactcctca
      361 ggacgtggat cgtgctgcgg acaaggtgtc caatctccgg atcttcgcag atgagggcgg
      421 tctcatgaac cggtcgatca tcgatatcgg tggctcggct ttggtcgtga gccagttcac
      481 gttgatcagc gacatgcgga gagggcgtcg accttcgttc gttggcgccg ccgacccggg
      541 aacggcggcg ccattggtgg agcgtctcgc ggagatgttg cgggacgccg ggattcacac
      601 cgagaccggc gtgttcggag cctcgatggc ggtcgacctg gtcaacgacg ggccggtgac
      661 cgtcatcctc gagattcgag agggcagagt cagttgatcg ggtgtcgggc gtcgtagaag
      721 tgcttcggga acaggaggag cgtgatgccg accgccgcga tcaatagcag tcgaggctct
      781 tcgacacccc aggcataggt gataccgacc tgatcccagg ccagggcgct ggcaatgagc
      841 acgacgaggg tggcgagggt tgccctgcca ctggacttgg tcagtgccca tgacacgatg
      901 atcaccacca ccaccacggg gaagatcacg ggccagatcc cgagcaagac accgccggtg
      961 atgctcagtc cctttccgcc ttcgaaccgg aaccagacgt tgtagacgtt cccggcgatg
     1021 gcaccgagac cggcggccac cgcacctgga tcgtcgccta tggccagccc caccgccact
     1081 gcggccaatc ccttgaccag ttccatcacc agcacgagag cggccagggg gtagcccccc
     1141 agggcacgag cgttgttggc gccggggttc gtcgtgccgt cggccctcag atcgacaccc
     1201 cacagccggc cgagccacac cgaggtgggg atggatccga ccaggtatcc caggatcagg
     1261 gccagggcaa tcatccgtcg gccacccact gctgcccggc ctcacagaac ctgaggaagt
     1321 cgcatcgccg gcaccattcg ccgggcgtcg gctcgaaacg ggaatcctcg atggcgtcgg
     1381 tgagtcgggt gaggctctgc tcggcccggc ctcgccatgt gtcgtcggcc cgttcggtct
     1441 cgacggccaa tccgcctccc aggtaggcga acgacacgtc gatctgctcc ggcttgtcga
     1501 ctccgaagtc gaccttcgtc gccgccatgg cgtatgcctg gagctggacg atgagtgagg
     1561 ggtcgtctcg gggtcgaccg ctcttgaagt cgacgatct
//
LOCUS       DATITW010000637         1828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_151070, whole genome shotgun sequence.
ACCESSION   DATITW010000637 DATITW010000000
VERSION     DATITW010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151070"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VLS86_08875"
     CDS             <1..623
                     /locus_tag="VLS86_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HSO50649.1"
                     /translation="FEGAQGTLLDIDHGTYPFVTSSNPTAGGASVGSGIGPKAIDEVI
                     GVAKAYISRVGTGPFPTELHDEIGEAMIELGGEYGTVTGRRRRCGWLDLVALRYAHRV
                     NTFTSLFLTKMDILSAFDTIKVAVAYTAEGDRHQHFPAQQSVLYHCEPEYHELPGWGV
                     DITSVRSYRDRPGEARAYVEFVQEAVEVPIRWVSVGPERSQLIEIS"
     gene            620..>1828
                     /gene="purD"
                     /locus_tag="VLS86_08880"
     CDS             620..>1828
                     /gene="purD"
                     /locus_tag="VLS86_08880"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HSO50650.1"
                     /translation="MKVLLIGGGGREHAIGWKLAASPRVTELISLPGNPGLARLGPVV
                     EAISANDVGAIAAMARVQGVDLVVIGPEGPLAEGAADAVATLGIPVFGPTRAAARLES
                     SKAFAKQVMSRAGVATATSETFIETAGAKEYLARTDGPFVVKADGLAAGKGVLVTTSR
                     DEAAAWVDHCLDGAFGSAGASVLVEEYLDGPEVSVFAVCTESGAVPLQPARDFKRLLD
                     GDQGPNTGGMGAYSPVPDLPPGLTETTMRDVIEPTLAQMIEEGSPFRGFLYAGLVLTD
                     DGPKVLEFNVRLGDPETQAVLPRLESDLVDVLEGAHPLWSAAATVNVVLAAAGYPETP
                     RSGDVIVGIESVPDDVLVFHAGTAAKGAKLVVDGGRVLNLVGSGPTLDAARARAYEAV
                     SAVRWPGMQYR"
BASE COUNT          284 a          582 c          653 g          309 t
ORIGIN      
        1 tattcgaagg ggcgcaggga acgctgctcg acatcgatca cgggacgtat ccgttcgtca
       61 cttcgtcgaa tcccacggcg gggggcgcct ctgtcggctc gggcatcggt cccaaggcca
      121 tcgacgaggt gatcggggtg gccaaggcat acatctcccg ggtaggcacc gggcctttcc
      181 ccaccgagct ccacgacgag atcggtgagg cgatgatcga gctgggtggt gagtacggga
      241 cggtgaccgg gcgacggcgg cgctgtggct ggctcgacct cgtcgccctc cgctacgccc
      301 atcgagtcaa caccttcacc agcttgttcc tgaccaagat ggacatcctg tccgccttcg
      361 acacgatcaa ggtggcggtc gcctacaccg ccgagggcga ccgtcatcag cactttcccg
      421 cccagcagag cgtgctctac cactgtgagc cggagtacca cgagctaccc ggctggggcg
      481 tggacatcac atcggtcagg tcgtaccgcg accgtcccgg cgaagccagg gcttatgtgg
      541 agttcgtcca ggaggcggtc gaggtcccga ttcgctgggt gtcggtcggg ccggagcgca
      601 gccagctgat cgagatctca tgaaggtcct cctcatcggc gggggcgggc gcgagcacgc
      661 catcgggtgg aagctcgccg cctcgccccg ggtcaccgag ctgatatcgc tgccgggtaa
      721 cccggggctg gcgcgactgg gaccggtggt ggaagcgata tccgccaacg acgtgggggc
      781 gatcgcagcc atggcccggg tgcagggagt ggacttggtt gtgatcgggc ccgaggggcc
      841 gctggcggaa ggcgccgccg acgccgtggc caccctcggg atcccggtct tcgggccgac
      901 gcgggccgcg gccaggctgg aatcctcgaa ggccttcgcc aagcaggtca tgtcccgggc
      961 cggtgtggcc accgccacct cggagacttt catcgaaacg gccggtgcca aagagtatct
     1021 ggcccgaacc gacggcccgt tcgtggtgaa agccgacggg ctggccgcgg ggaagggagt
     1081 gttggtcacc acgtcgcgtg acgaggctgc cgcctgggtg gaccactgcc tggacggagc
     1141 cttcggctcg gccggggcgt cggtgctggt cgaggagtat ctcgacggtc ccgaggtcag
     1201 tgtcttcgca gtctgcaccg agagcggagc cgtcccgctg cagccggccc gggacttcaa
     1261 gcgtctgctc gacggcgatc aggggccgaa taccggtggc atgggtgcct actccccggt
     1321 ccccgatctt cccccgggtc tgactgaaac gacgatgcgc gatgtcatcg agccgaccct
     1381 ggcccagatg atcgaggagg gaagcccgtt ccgtgggttt ctctatgcag gtctggtcct
     1441 gaccgacgac gggccaaagg tgctcgagtt caacgtgcgt ctcggcgacc cggagacaca
     1501 agcggtgctg ccccgtctcg agtccgacct cgtcgatgtt ctcgaaggtg cccatccact
     1561 ctggtcggcc gcggcgacgg tcaacgtggt gctcgccgct gctggctacc ccgagacccc
     1621 gaggtcgggc gatgtcatcg tcggaatcga gtccgtgccc gacgacgtcc tggtgtttca
     1681 tgccggcacc gcggcgaagg gggcaaagct ggtggttgac ggtggccggg tgctgaacct
     1741 ggtcggcagc gggcccacgt tggatgcggc cagggcaagg gcctatgagg cggtctcagc
     1801 cgttcgctgg cccgggatgc agtatcgg
//
LOCUS       DATITW010000638         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_752167, whole genome shotgun sequence.
ACCESSION   DATITW010000638 DATITW010000000
VERSION     DATITW010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752167"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VLS86_08885"
     CDS             complement(<1..958)
                     /locus_tag="VLS86_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007980795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSO50651.1"
                     /translation="MTSIASFAFLVALLPVAALAPGGTFTDDDGIVHEGGIEAIATEQ
                     ITEGCNPPFNDRFCPDRLLTRAEMATMVARALSLPGAAIDHFVDDDGHVLEGAINRVA
                     EAGITVGCNPPANDRFCPNRNLSRAEAAGFLARALGLPASTRDFFDDDEGNVLEGAIN
                     RLAEAGITVGCNPPANNNFCPSRILTRAEMATMLTRSLQLTSMKPPARPPLDWDLVVD
                     GLDGPIQALSPPGESRILIAELGGLIRVFENGALRAAPFLDLSDTVEVGGEKGLLSIA
                     VHPDYPVDRRLFAWYSAPLRAGGSGNHTTFLVEFDIAADLQTA"
     gene            1020..>2017
                     /locus_tag="VLS86_08890"
     CDS             1020..>2017
                     /locus_tag="VLS86_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSO50652.1"
                     /translation="MPIALAIALTLSLFPWSPQTDDAPGTVRLLVADDVSIGPADAGE
                     ASDGDSRWHRVEVPIRGSVEQTAASLAAESGSRVIVEQRYELLAPEDDPRFAEQWYLE
                     NGGQSGGVPDADIDALTAWGSGLGNGIVVAVIDSGVNMAHTDLVGQIHSARRDFVDND
                     NNPSPSGTTDDEGHGTAVAGIIAAAENGLGITGVAPEATIMAIRSCSQGACWSFDIAE
                     GIYYAADNGADVINLSLGSVTPQGDEPLEDAIAYARSRDVVVVAAAGNSGTNLDDLDP
                     GEQLIPGGLPFSNILTVGATNRRDNLAGFSNYGPGVVDIAAPGVDILTTGISSNTS"
BASE COUNT          374 a          682 c          656 g          305 t
ORIGIN      
        1 aggccgtttg cagatcggcc gcgatgtcga actcgacgag aaaggtcgtg tggttgccac
       61 ttccgcccgc ccggagcggc gccgagtacc aggcgaacaa cctgcggtcg accgggtagt
      121 cgggatgaac cgcgatcgac agcaggccct tctccccacc aacctcgact gtgtccgaga
      181 gatcgaggaa tggcgcggct cgcagggcgc cattctcgaa gacgcggatc agcccaccca
      241 gctcggcaat caagatccga ctctcacccg gaggcgacag ggcctggatc gggccgtcca
      301 agccgtcgac gaccaggtcc cagtcgagcg ggggacgagc cggcggcttc atcgacgtca
      361 gctggagcga ccgggtgagc atcgtcgcca tctcggcccg ggtcaggatc cggctcgggc
      421 agaagttgtt gttggccgga gggttgcagc ccactgtgat ccccgcctcg gcgagacggt
      481 tgatcgcccc ttcgagcaca ttgccttcgt cgtcgtcgaa gaaatcccga gtcgacgccg
      541 gaagacccaa ggcgcgcgcc aggaacccgg cagcctcggc gcgcgacagg ttccggttgg
      601 ggcagaaccg gtcattggcc ggggggttgc acccaaccgt gatcccggcc tccgccacgc
      661 gattgatcgc cccctcgagc acatgaccgt cgtcgtccac gaagtggtcg atggcggcgc
      721 cggggaggga gagcgcccga gccaccatgg ttgccatctc ggcccgggtc aggagacggt
      781 cggggcagaa tcggtcgttg aacggagggt tgcagccctc ggtgatctgc tcggtggcaa
      841 tggcctcgat cccgccttca tggacgatcc cgtcgtcatc ggtgaaggtc cctcccgggg
      901 cgagcgcggc caccgggagc aaggcgacaa ggaaagcgaa tgaagcgatg gaagtcatcg
      961 accggtgaca aggtaaccac ttctctcgcc aaagcgccag agtctgggag tattggccga
     1021 tgccaatcgc cctggccatc gccctgactc tctccctctt cccgtggtcg ccacaaaccg
     1081 acgacgcacc gggaaccgtg aggctgctgg ttgcagacga cgtgtccatc ggcccggccg
     1141 acgccggcga agcgagcgac ggcgactcgc gctggcaccg ggtcgaagtg ccgatccggg
     1201 gaagcgtcga gcagactgcc gccagtctcg ccgccgagtc cggctcccgt gtgatcgtgg
     1261 aacagcgcta cgagcttctc gcccccgagg acgacccgag gttcgccgag cagtggtacc
     1321 tggagaacgg cggtcaatcg gggggtgtcc ccgacgccga catcgatgct ctcaccgcat
     1381 ggggatcggg gctcggcaac ggcatcgtcg ttgccgtcat cgactccggc gtgaacatgg
     1441 cccacaccga tctcgtcggg cagatccatt cggcgcgccg cgatttcgtc gacaacgaca
     1501 acaaccccag ccccagcggg accacagacg acgagggtca cggcaccgct gtggccggga
     1561 tcatcgccgc cgccgagaat gggctcggga tcaccggggt cgcaccagag gccacgatca
     1621 tggcgatccg aagctgtagc cagggagctt gctggagctt cgacatcgcg gaggggatct
     1681 actacgcggc cgataacgga gccgacgtga tcaatctctc gctgggatcc gtcaccccac
     1741 agggcgacga gcctctcgag gatgccatcg cctacgcccg ctccagagac gtggtcgtcg
     1801 ttgccgccgc cggcaacagc ggcaccaacc tcgatgacct cgatccaggc gagcagctca
     1861 tcccgggagg gctccccttc tccaacatcc tcactgttgg cgcaaccaac cgacgcgata
     1921 acctcgccgg gttcagcaat tacggccctg gcgttgtcga catcgcggca cccggggtcg
     1981 acatcctcac caccgggatc agcagcaaca ccagcta
//
LOCUS       DATITW010000639         1971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_671694, whole genome shotgun sequence.
ACCESSION   DATITW010000639 DATITW010000000
VERSION     DATITW010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671694"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1548
                     /locus_tag="VLS86_08895"
     CDS             52..1548
                     /locus_tag="VLS86_08895"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HSO50653.1"
                     /translation="MAQIAHWINGKPWEGAPTRTGKVYDPATGVIQSEVALATPEVVN
                     AAVAAAGEAFPAWRDSPITRRQNVMFAYRELVVENRHEIAKLITAEHGKTTPDALGEV
                     QRGLEVIEFACNIAHMVKGEFSEQVSTGVDTFTMRQPLGVVAGITPFNFPAMVPMWMY
                     PLAIAAGNTFILKQSEKDPSPSMFCAELLKEAGLPPGVFNVVHGDKVAVDRILEHPDI
                     QAVSFVGSTPVAHYIYENGTKAGKRVQALGGAKNHMVVLPDADMDLAADAAVSAGYGS
                     AGERCMAISVVVAVGDAADRLIPEVVARIAKLRTGPGSDPDSEMGPLVTPEHRDKVVG
                     MIDKGVEEGADLLIDGRGYSPDGFESGFYVAPTLFDKVSPKMEIYKHEIFGPVLSVVR
                     VDRYQDAIDMINENPYGNGTALFTNDGGAARKFQSEIQVGMVGINVPIPVPLAFYSFG
                     GWKSSIFGANPIYGPEGVRFYTKQKVVITRWPDPIYRGVDLGFPQHGK"
BASE COUNT          388 a          607 c          618 g          358 t
ORIGIN      
        1 actcgggcca ccgactcatc cgggcgtaca atcgaatcaa aaggaaggca gatggctcaa
       61 atcgcacatt ggatcaacgg aaagccctgg gaaggggccc cgacccggac cggaaaggtc
      121 tacgaccccg ccaccggagt aatccagagc gaggtggcgc tggcgacccc tgaggttgtc
      181 aatgctgccg tggccgcggc cggggaggct tttccggcct ggcgcgactc ccccatcacc
      241 cggcgccaga acgtgatgtt cgcctatcgg gaactggtgg tcgagaatcg gcatgagatc
      301 gccaaactga tcaccgccga gcacggcaag accacacccg acgccctcgg ggaagtccag
      361 cgtgggctcg aggtcatcga gttcgcctgc aacatcgccc acatggtcaa gggcgaattc
      421 agcgagcagg tttcgacggg ggtggacaca ttcacgatgc gccagccgct cggggtcgtc
      481 gccggtatca cccctttcaa ctttccggcc atggtcccga tgtggatgta cccgctggcc
      541 atcgccgccg ggaacacctt catcctcaaa cagtccgaga aggacccctc tccgtcgatg
      601 ttctgcgcag agcttctcaa agaggcgggc ctgccacccg gggtcttcaa cgttgtccac
      661 ggcgacaagg tggcggtcga tcggatcctc gagcaccccg atatccaggc ggtctcattc
      721 gtcggctcta cgccggtcgc ccattacatc tacgagaacg gaacgaaggc cggaaagcgg
      781 gtgcaagccc tgggcggggc caagaaccat atggtggtgc tgcccgacgc cgacatggac
      841 cttgccgccg atgcggccgt ctcggcgggc tacggatctg ccggtgagcg atgcatggcc
      901 atatctgtgg tcgtcgcggt cggcgatgcc gccgatcggt tgattcccga ggtcgtggca
      961 cgcatcgcca aactccgcac cggaccaggc agcgatccgg attcggagat gggaccgctg
     1021 gtgactcctg agcaccgcga caaggtcgtc ggcatgatcg acaagggtgt cgaggaaggc
     1081 gccgacctct tgatcgacgg acgcggctac tcgccggacg gcttcgagag cgggttctac
     1141 gtggccccga ccttgttcga caaggtctcg ccaaagatgg agatctacaa gcacgagatc
     1201 ttcgggccgg tgttgtcggt ggtgcgggtt gaccgttatc aggacgccat cgacatgatc
     1261 aacgagaacc cgtacgggaa cggaaccgct ctgttcacca atgacggtgg cgccgcccgc
     1321 aagttccaga gcgagatcca ggtggggatg gtcgggatca acgtgccgat tccggtgccg
     1381 ctcgccttct attccttcgg cggatggaag tcgtcgatct tcggggccaa cccgatctac
     1441 ggaccggaag gggtgcgctt ctacaccaag caaaaggtcg ttatcacccg ttggcccgac
     1501 cccatctacc gcggcgtcga cctcggcttc ccccaacacg gaaagtaaaa aaccgcgaac
     1561 ctaaggtttt aggccgcggt ttccgcggcc taacgcgtat tggtttcaag gatctagacc
     1621 gcgggttcga ggcggcgcat gctttgacga tgcttgacgc aaccgaactt cgtgtcatgg
     1681 aactcgccag ccgccaatca gggtatgtaa cccgacgtca gtcgatcgcc tgcggaatga
     1741 gtgcggacgc aattgaccga ctggtgaact cgaagcggtg gagtcggatc aaaccgtggt
     1801 tgtacctggt ttcgggctat tccccgagta ctcgcgggct gctggtcgca gcgacggcga
     1861 ctcttggggc tgtcgtgtct catgaatccg cagctgaaat gcacgacctt ccgggaccag
     1921 ccagaggtca cgcggttgtc accctgcgcg tgcgaacgac ctatcggttt c
//
LOCUS       DATITW010000640         3472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_71674, whole genome shotgun sequence.
ACCESSION   DATITW010000640 DATITW010000000
VERSION     DATITW010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71674"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VLS86_08900"
     CDS             <1..322
                     /locus_tag="VLS86_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSO50654.1"
                     /translation="GVADLVDRCCGLARRLAEGLGNLDGIEVVNDVVLNQVLVRVGDA
                     DLTDRVERHIQAEGECWLGATTWRGERLLRISVSNWSTTPADIDRSIDSIGRARADLV
                     GDSP"
     gene            428..1042
                     /locus_tag="VLS86_08905"
     CDS             428..1042
                     /locus_tag="VLS86_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50655.1"
                     /translation="MASVAFLLAACAGADTGVVTFTDPEKLSKIDIPESWNVYQLAEL
                     SALEDLPFDEQVQGFSFPAISSVGFDGGPVRDVSNLASSLPEAEYPIGVMSVRQVGEI
                     EKDLLSRAMLTQSVLPYYGLTNSEEITKEDFSFGSGFDGVRVLVAFQSDTAVDQGVAY
                     LISVSNADDTRIYSMIAGCNRDCFIANQETIEKVVDSWLVNKRA"
     gene            1018..3333
                     /locus_tag="VLS86_08910"
     CDS             1018..3333
                     /locus_tag="VLS86_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSO50656.1"
                     /translation="MARKQKGLNTRKRDIRSTRRRADMWDRIKILVLLGVIIGAIIAS
                     SDDLAFLSLWEVALEFFSSPAGASLGILFVLEVARQIHYWFSERSAGYNSFWQESVFG
                     GMERWTHSHIKPWTRFRVARFIRRMVYLFVFALLIDYFVDDVNGPLEAIVEAPRIFAD
                     AFPFLLQLSFGFLFVAIQFLGIFWLLSRGGTDVILPEEVTTRFDDVWGQDHVLDLIKE
                     NIAFLEKPDEIEERGGYIPGGILLWGPPGTGKTLMAQAVAGETGRPYVFVEPGAFINM
                     FFGIGLLKVKMLYRKLRKLSLRHGGVIVFFDEADGLGSRGQMGSPQGQFGSAVKSFAG
                     LDCNGFNYLSAQTQDLLVNYWTAEEPAPASPQKPGFVDRIMMGGMGMGGGGMGVLQAL
                     LTEMNGLTKPRGMTNKLRKLLGFRPKPPPRYRLLHIMATNMPNALDEAMLRPGRIDRI
                     YKVGYPSKEGRRQTIVGYLNKVEHHLSEDEVDRLATVTPYYSGAKIKDMVNEALIIAV
                     RDGREAIEWRDIWKAKSLKELGPPEDVDYIQRERHAVAIHEASHAVTAHLLGAHRRID
                     MVSIEKRASTLGMVKSMGQEERFTQWKSEMETDIMVSLASLAGERLFFAGDNSSGVSG
                     DLSSASTVAGLMEGSFGMGQGRASAMGTRETGSGGPANPVSIALRERRPEIEVILIDL
                     YQRVGELLEEHQEKILELAAVLEEKKTISGEEVAEIMGSVPGSRTMREPKGWQAVSDE
                     VAGERLRAALHRTGREVAATPTVDSKEAVEP"
BASE COUNT          641 a         1091 c         1109 g          631 t
ORIGIN      
        1 tggagtcgcc gatctggtgg acagatgctg tgggctggct cgccgtctgg ccgaaggtct
       61 gggaaacctc gacggcattg aagtcgtcaa tgacgtggtg ctgaatcagg ttctggtgcg
      121 ggtcggcgac gccgatctca ccgatcgggt cgagcgccac atccaggctg aaggggaatg
      181 ctggctaggc gccaccacct ggcgtggaga gcgtctgctt cgaatctcgg tctccaactg
      241 gtcgacgacg ccggcagaca tcgaccgcag catcgactcg atcggcaggg ctcgtgccga
      301 cctggtcggg gactcaccct gagtcgagcg cccacctgac atttcgaaag gaacctgctg
      361 caaaacgcac aaacggttct aatcttccgc gatcaaccgg atcgcaccag tacgcacacg
      421 attgaccgtg gccagcgttg ccttcctgct cgctgcctgt gccggcgcgg acaccggcgt
      481 cgtcaccttc accgacccgg aaaagctgag caagatcgac attcccgagt cgtggaacgt
      541 ctatcaactc gccgagctgt cggcactgga ggatcttccg ttcgacgagc aggtccaggg
      601 attcagcttt cctgccatct ccagtgtcgg cttcgacggt gggccggtcc gcgacgtgag
      661 caacctcgcc tccagtcttc ccgaggccga gtacccgata ggggtgatgt cggtgcggca
      721 agtgggtgag atcgaaaagg acctgttgtc ccgggcaatg ctcacccagt ctgtgcttcc
      781 ctactacggg ctgaccaaca gcgaggagat aaccaaggaa gacttcagct tcggcagcgg
      841 cttcgacggt gtccgggtgt tggtcgcctt ccagagcgac accgccgtgg atcagggggt
      901 ggcctatctg atctcggtgt ccaatgccga cgacacccgc atctactcca tgatcgcggg
      961 ctgcaaccgg gactgcttca tcgccaatca ggaaaccatc gagaaagtgg tcgactcatg
     1021 gctcgtaaac aaaagggcct gaacacccgc aaacgtgaca tccgctccac ccggcgtcgg
     1081 gcggacatgt gggaccggat caagatcctc gtcctgctcg gcgtcatcat cggagcgatc
     1141 atcgccagca gcgacgatct cgccttcctc agcctgtggg aggtcgcgct cgagttcttc
     1201 tccagccccg ccggtgcatc cctcggcatc ctgttcgtgc tcgaggttgc ccgtcagatc
     1261 cactactggt tctccgagcg ctcggccgga tacaactcct tctggcaaga gagcgtcttt
     1321 ggcggcatgg agcgatggac ccatagccac atcaagccgt ggacgcggtt ccgcgtggcc
     1381 cgcttcatcc gacggatggt ctacctcttc gtgttcgccc tgctcatcga ctatttcgtc
     1441 gacgacgtca acgggccgct cgaggccatc gtcgaggcgc cacggatctt tgccgacgcc
     1501 ttccccttcc tcctccagct ctcgtttggc ttcctgttcg tagccattca gttcctcggg
     1561 atcttctggc tgctctcgag gggcggcacc gatgtgatcc tccccgagga ggtgaccacc
     1621 cgcttcgacg acgtctgggg tcaggaccat gtcctcgacc tgataaagga gaacatcgca
     1681 tttctcgaga agcccgacga gatcgaggag aggggcggat acatcccggg tggcatcctc
     1741 ctctggggcc cgccggggac gggcaagacc ttgatggctc aggcagtggc cggcgagacc
     1801 gggcggcctt acgtcttcgt cgaaccaggc gcattcatca acatgttctt tggcatcggg
     1861 ctgctcaagg tcaagatgct gtaccgcaag ctgcgcaagc tcagcctccg tcacggcggg
     1921 gtcatcgtgt tcttcgacga ggccgacggg ttgggctcga ggggacagat gggctccccg
     1981 cagggccagt tcggctcggc ggtcaagtcc ttcgccgggc tcgactgcaa cgggttcaat
     2041 tatctcagcg cccagactca ggaccttctg gtcaactact ggaccgcaga ggagccggcc
     2101 ccggcctcgc cgcagaagcc cgggttcgtg gaccggatca tgatgggcgg gatgggcatg
     2161 ggtggcggcg gcatgggcgt gctccaggcc ctgctcaccg agatgaatgg gctcaccaag
     2221 ccgaggggca tgaccaacaa gctgcgcaag ctgctcgggt tccgtcccaa gccgcccccc
     2281 cggtatcgcc tgctccacat catggcgacc aacatgccga atgccttgga cgaggccatg
     2341 ctccgccccg gtcgcatcga tcgcatctac aaggtcgggt atccgtccaa agagggacgg
     2401 cgccagacca tcgtcggcta tctgaacaag gtggagcacc acctgagtga ggacgaggtg
     2461 gaccgtctcg ccaccgtcac tccctactac tccggggcca agatcaagga catggtcaac
     2521 gaggccctga tcatcgcggt gcgcgacggt cgggaggcca tcgaatggcg ggacatctgg
     2581 aaggccaagt cgctcaagga gctgggcccg cccgaggacg tggactacat ccagagggag
     2641 agacacgcgg tggcgataca cgaggcaagc cacgcggtga ccgcgcacct gctcggggca
     2701 catcgtcgca tcgacatggt ctcgatcgag aagcgcgcct ccacgttggg gatggtcaag
     2761 tcgatgggtc aggaggagcg gttcacccag tggaagtcgg agatggagac cgacatcatg
     2821 gtctccctcg cctcactggc cggtgagcgt ctcttcttcg caggcgacaa cagctcggga
     2881 gtctccggcg acctgtcctc ggcatcgacg gtcgccggtc tgatggaggg ctcattcggg
     2941 atgggtcagg ggcgggcgtc ggcaatgggg accagggaga ccggctcggg cgggccggcc
     3001 aacccggtct cgatcgccct tcgcgagcgt cgccccgaga tcgaggtgat tctgatcgac
     3061 ctctaccagc gggtcggtga actcctcgaa gagcatcagg agaagatcct cgagttggct
     3121 gccgtcctgg aggagaagaa gacgatctcg ggcgaggaag tggccgagat catggggagc
     3181 gtcccgggct cacgaaccat gcgcgagccc aagggatggc aggcagtgtc cgatgaggtt
     3241 gccggcgagc gtctacgcgc cgccctccat cgcaccggac gcgaggttgc ggcgacgccg
     3301 accgtcgaca gcaaggaagc cgtcgagccc tgatctaccg ctgatcaacc gagtggagct
     3361 cagccgtcct gccaccgggg gtcgccgatg cgaccgatcg ggcagttctc ggcgagaatc
     3421 ctgacttcct cgtcgcggtc cagatcgtcg aggatcaggc tttgaccgtc ga
//
LOCUS       DATITW010000641         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_671716, whole genome shotgun sequence.
ACCESSION   DATITW010000641 DATITW010000000
VERSION     DATITW010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671716"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..924
                     /locus_tag="VLS86_08915"
     CDS             163..924
                     /locus_tag="VLS86_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50657.1"
                     /translation="MTAPILDLRKVTKVFGGVTAVREVNMSVEAGSITAVIGPNGAGK
                     TSLFNVSTGFEEPTEGQVLFEGEDVTGLEPWRVARKGLIRTFQTPTGFPALSVWENLM
                     VGGTSDRDESLWRAMRGAWHSGERTTNQRARQVLTDLGLWDRRDTLLADLSPGDVKLV
                     DFARQLMAEPKMVLLDEPASGVDPASIGRLGSVIRQVRERNVTVLVIDHNIGFVLGIA
                     DQVHVMALGQVIASGSPDDVVRDPVVIEIYLGRSA"
     gene            921..1637
                     /locus_tag="VLS86_08920"
     CDS             921..1637
                     /locus_tag="VLS86_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50658.1"
                     /translation="MSLLELDQLETGYGRLPVLHSVTLRVETNEMVAIIGPNGAGKTS
                     LAKAVFGVLPAMRGTVRFDGEDLTSATTARRIAMGMAMAPQVGNTFPELTVEDNLIVS
                     FSTLGSDDAERALASAYAMFPRLEERSRQAARTLSGGERQMLAFASCSGSNPRFLLLD
                     EPTTGLAPTIVHSLVEKIIDFKEKGASILWVIEENPLEVLPYVDRVYVLSNGAIQAET
                     AAKDLLTEEALSSLFFGLEQ"
     gene            1634..>2692
                     /locus_tag="VLS86_08925"
     CDS             1634..>2692
                     /locus_tag="VLS86_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HSO50659.1"
                     /translation="MTADILFDTGAIGGLRLRNRILRAGTSESMAEPASGSMTAPLLD
                     LYERLAAGGVGGIITGHLFVAARGKYARGQTGVHDDEMVPGLTRLTERVHSHGSAILA
                     QLAHAGSQSRVPDNLPVAPSPVPNPLTGNPVEAASSDEISATVEAFGQAARRAVEAGF
                     DGVHIHGANGYLISEFGSPVTNRRDDQWGGDSERRSAFSKAVVQEVRRSVPPGFPVTM
                     KIGMVDAVPDGLDIDESVARADSLVDLGLDAVEVSCGLMQAPTDSANTYVAVGPKRAA
                     QDLLVHRLLSAPRPEAYFRPWAQALRARVDTKIILTGGMRTTGTMRQVLESSDADFIG
                     LARPFIREPDLVSQILRGR"
BASE COUNT          485 a          832 c          866 g          509 t
ORIGIN      
        1 agccgcctca ctggtacagg gctctggtga catggcggtg gtccttgctt cggtcagatt
       61 tatcgtgctt ggactcgtcc tcatcctcgt gatgcgggtg cgacctcaag ggttggccaa
      121 cgagcgcgac gcattcgccg gagcatcgga aagggtgccg ggatgactgc gccgatcctg
      181 gacctgcgaa aagtcaccaa ggtcttcggt ggggtgactg cggttcggga agtgaatatg
      241 tccgttgaag ccggatccat caccgcggtc atcgggccaa atggcgccgg gaagacgtcg
      301 ctgttcaatg tttccaccgg attcgaggaa ccgacagagg gacaagtcct ctttgagggc
      361 gaggatgtga ccgggctcga accgtggcgt gtggctcgga aggggctcat ccgaaccttc
      421 cagactccga ccgggttccc cgctctctcg gtatgggaga acctgatggt cggcggaacc
      481 agtgatcgcg atgagagcct ttggcgggca atgcggggcg cctggcactc cggtgagagg
      541 accaccaatc aacgcgcccg acaggtgctg acagacctcg ggctctggga tcgtcgcgat
      601 actctcctcg ccgacctgtc cccgggcgat gtcaagttgg tggacttcgc ccgccaattg
      661 atggccgagc ccaagatggt ccttctcgac gaaccggcct ctggcgtcga cccggcttcg
      721 atcggtcggc tgggctccgt gatccgtcaa gtcagagaac ggaatgtgac tgtcctggtg
      781 atcgatcaca acatcggatt cgtgctcggc atcgccgatc aggttcacgt catggcgttg
      841 ggtcaagtca tcgcctccgg ctcgcccgat gatgtagtcc gggatccggt ggtaatcgag
      901 atctatctgg gcagatcggc atgagtttgc tcgagctaga ccagttggaa accggctatg
      961 gccggcttcc tgttcttcat tctgtcacgc tgcgggtcga aaccaacgaa atggtggcga
     1021 tcatcggtcc caacggcgcg gggaagacat ctctggccaa ggcggtcttc ggcgtgctcc
     1081 ccgcgatgcg agggacggtc cgcttcgatg gcgaggacct gacttcggcg accactgccc
     1141 gtcgaatcgc aatggggatg gcgatggcgc cccaggtcgg caacactttt cccgagctga
     1201 cagtcgagga caacctgatc gtgtccttct ccaccctcgg atcggacgat gcggagcggg
     1261 ctctggccag cgcctacgcc atgttcccac gactcgaaga acggagcaga caggccgctc
     1321 gcacgctgtc cggaggtgag cggcaaatgc tcgccttcgc cagttgctcc ggctcgaacc
     1381 ccaggtttct actcctcgac gagccgacca cagggctggc tcccacgatc gttcactccc
     1441 tggtggagaa gatcatcgat ttcaaggaga agggcgcttc gattctctgg gtcatcgagg
     1501 agaatccgct ggaggtgctc ccctacgtcg accgcgtgta cgtgctgagc aacggggcga
     1561 tccaggcgga aacagcggcc aaggacctcc tcaccgagga agctctcagc tcgctcttct
     1621 tcggactgga gcagtgaccg ccgacatcct cttcgataca ggcgcgatcg gagggttgag
     1681 gctgcgaaac cgcatcctcc gagccggtac ctcagagtcg atggccgaac ccgcttcggg
     1741 atcgatgacg gcgccgctct tggatctgta tgagcgtctt gccgccgggg gggtgggggg
     1801 catcatcacc ggacatctct tcgtcgccgc tcgaggcaaa tacgccaggg gccaaaccgg
     1861 tgttcacgac gatgagatgg ttcccggctt gaccagactg accgagcggg tccactcgca
     1921 cggctcggcg attctcgctc aactggccca cgcaggatcc caatcacgag tccccgacaa
     1981 cttgcccgtc gcaccatcgc cggtcccgaa tccgctgact ggcaacccgg tggaggcggc
     2041 ctccagcgac gagatctccg ccacggtcga ggcattcggc caggcagcac ggcgagcggt
     2101 cgaggccggt ttcgatggcg tgcacattca cggagcgaac gggtatttga tcagcgagtt
     2161 cggctcaccc gtgaccaatc gccgggatga ccaatggggt ggtgacagcg aacgccgcag
     2221 cgcgttctcg aaggcagtag tgcaggaggt tcgccggtcg gtcccgccgg gtttcccggt
     2281 gacgatgaag atcggcatgg tcgatgccgt gcccgacggt ttggacatcg acgagtcggt
     2341 ggccagggcg gactccctcg tggacctagg actggatgcg gtcgaagtct catgtgggct
     2401 gatgcaggct cccaccgact ccgccaacac ctacgtcgcg gtggggccaa aacgagcggc
     2461 tcaggacctg ctcgtgcatc ggctgctgtc cgcgcctcgt cccgaggcgt acttccggcc
     2521 ctgggcccag gctctccggg ccagggtgga cacaaagatc atcctcaccg ggggcatgcg
     2581 caccactggg accatgcgcc aagtgctcga gtcgtccgac gccgacttca tcggtctggc
     2641 tcgaccgttc attcgagagc ccgatctcgt ttcccagata ctcagaggcc gg
//
LOCUS       DATITW010000642         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_552384, whole genome shotgun sequence.
ACCESSION   DATITW010000642 DATITW010000000
VERSION     DATITW010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552384"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VLS86_08930"
     CDS             <1..1034
                     /locus_tag="VLS86_08930"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HSO50660.1"
                     /translation="LGIPIPHTALIPRSKDEIGRGLAEFVHQNFLDPTSLSKWIIDAD
                     IAGRMATWLDSSDHAALAARRSIETAATMAASLDDERVASVVTQTSLDWLRQTPVTPL
                     ISLVVDAWLKSGRTQESIEAGLKGIEVVLVDNLPYFKESFARSKPWWVPTWAEDLLFE
                     RIIASFRVLVSDVSADPAHPIRSDIERMLASLADDLRNSEDLAQRIEESKLRILDSPE
                     LAAAIQSQWQLLHESIERAVDQPSSELENRLAELIQWWSRRVLEDAPLRMRIDSWIAE
                     SAERLANKWDVEVIAMIETTVAGWDATEISHRLEMQLGRDLQFVRINGTVVGGLVGIA
                     IHGLLLLTG"
     gene            complement(1053..1418)
                     /locus_tag="VLS86_08935"
     CDS             complement(1053..1418)
                     /locus_tag="VLS86_08935"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HSO50661.1"
                     /translation="MSLSAEQLDLVSRANEVLIETRSGDRVFQTVIWVVVDGEDVLVR
                     SVRGESGRWYQRALANPEVALIVGNTRLEFTAVPASDEVSVERASEALRRKYRVGRSL
                     DAMLVPGVLGTTLLLAPLA"
BASE COUNT          286 a          524 c          494 g          253 t
ORIGIN      
        1 cgctggggat ccccatcccg cacaccgccc tcatcccgcg cagcaaggac gagatcggtc
       61 ggggattggc cgagttcgtc catcagaact tcctggaccc gaccagtctc agcaaatgga
      121 tcatcgacgc cgacatcgcg gggcgaatgg ccacctggct cgactcatcg gatcatgcgg
      181 cgctggccgc acgtcgcagc atcgagaccg cagccaccat ggccgcctcg ctcgacgacg
      241 aacgggtcgc ctcggtggtc acccagacct ccctcgactg gttgcgacag acaccggtca
      301 ccccgctcat ctccctggtt gtggacgcct ggctgaagag cggaaggacg caggagtcga
      361 tcgaggccgg cctgaagggg atcgaagtcg tcctcgtcga caatctgccc tacttcaagg
      421 agagcttcgc ccggtccaag ccgtggtggg tccccacatg ggccgaggat ctcctgttcg
      481 aaaggatcat cgcctcgttc cgggtgctgg tctcggatgt gagcgccgac cccgcccatc
      541 cgattcggtc ggacatcgag cggatgctgg cgagtctggc cgacgacttg cgcaactccg
      601 aggacctcgc gcaacggatc gaggagtcca agctgcgcat cctcgactcg ccggagttgg
      661 cggctgcgat ccaatcgcag tggcagctgc tccacgagag catcgagcgc gcggtggacc
      721 agcccagctc agagttggag aacaggttgg ccgagctgat ccagtggtgg tcccgccggg
      781 tactggagga cgccccgctc agaatgcgga tcgacagttg gatcgccgag tcggcggagc
      841 gtctcgccaa caagtgggac gtagaggtga tcgcgatgat cgagaccacg gttgccggat
      901 gggacgccac cgagatctcg catcgtctcg agatgcaact gggccgggac ctccagttcg
      961 tgcgcatcaa cggcaccgtg gtgggtggat tggtcgggat cgcgattcac ggtctcctgc
     1021 tcctgaccgg ctgacgaggg ctccgatcgg agtcaggcca ggggcgcgag gagcagagtg
     1081 gtgcccagta cccctggaac caacatcgcg tccagcgagc ggcccacccg gtacttgcgg
     1141 cgaagggcct ccgaggcccg ctccactgac acctcgtctg aggcgggaac ggccgtgaac
     1201 tcgagcctgg tgttcccaac gatcagagcc acttcagggt tggccagcgc ccgctgatac
     1261 caccgacccg attcaccgcg aaccgagcgc accagcacgt cttctccatc cacgaccacc
     1321 cagatcacag tttggaagac acggtcgccc gaccgggtct cgatcaggac ctcgttggcc
     1381 ctggagacca ggtccagctg ctcggcgctc aatgacatga tcgcaacgct aaccagacct
     1441 cgtcgattct gtcccacgca gaccgtaacc tgccaacggt gacagcaccc ggcccactcg
     1501 agcttggtcg ctccgtggtg gtgggcccgg cagcgcctgc acccccggcg tgggccg
//
LOCUS       DATITW010000643         1505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_151330, whole genome shotgun sequence.
ACCESSION   DATITW010000643 DATITW010000000
VERSION     DATITW010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151330"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VLS86_08940"
     CDS             <1..816
                     /locus_tag="VLS86_08940"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HSO50662.1"
                     /translation="DRVVNIHTIADEVDGTIVGAGQTFSLNDHVGQRTLEAGYLPAGT
                     IIAGEVEDTVGGGVSQFTTTMYNAVFWGGYEDVEHKPHSYYFSRYPEGIEATLSWRSP
                     DLAFRNNTDRAVLIDTRYTDTSITVRIFGSNDGRVMKGEQSGGNTSIWTDGEGGADAL
                     LVRGEVSDRYALTDPPPPRYEPNPELGVDDQDQTQTEEGGWSVTVTREIRRGEDVVDT
                     QEWGVRYAPRFAVFEVHPCKIPGTAVACPTTTTTATTTTVPTTTSSSATPPST"
     gene            complement(826..1131)
                     /locus_tag="VLS86_08945"
     CDS             complement(826..1131)
                     /locus_tag="VLS86_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50663.1"
                     /translation="MGQTVAIRDSVTMGDVLLVSTDRSFTGQDGQSITGDAPGDAVPG
                     LLATELLGLDIGIDHVYVQQNTISLRRPGGWDSESVAKSLEVTSSFLRHYPDDGEEE"
     gene            complement(1149..>1505)
                     /locus_tag="VLS86_08950"
     CDS             complement(1149..>1505)
                     /locus_tag="VLS86_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HSO50664.1"
                     /translation="EMAINLGATAIVAFSESGNTARLISKYRPEGRIVAFSPNESTLR
                     RMALFWGVSPHEFERRDYTDDEMAAASAILESEGLAKAGDTVVMVAGVPPNIQASTNL
                     VKVQVIGDHPRGFGGS"
BASE COUNT          287 a          508 c          469 g          241 t
ORIGIN      
        1 gaccgtgtcg tgaacatcca cacgatcgcc gacgaggtcg acgggaccat cgtgggcgcc
       61 ggacagacat tcagcctcaa cgatcatgtc ggccagagga cgctggaggc cggctacctc
      121 cccgccggca ccatcatcgc cggcgaggtc gaggacaccg tcggcggcgg ggttagccag
      181 ttcacgacga cgatgtacaa cgccgtgttc tggggtggct acgaggacgt cgagcacaag
      241 ccccactcct actacttctc ccgttacccc gaggggatcg aagccacgct gagctggaga
      301 agccccgatc tcgcgttccg caacaacacc gaccgcgcgg tgctgatcga caccaggtac
      361 accgacacct cgatcacggt ccgcatcttc ggaagcaacg acggcagggt gatgaagggc
      421 gagcagagtg gcgggaacac ctcgatctgg accgacggtg aggggggcgc cgatgccctc
      481 ctggtccgag gcgaagtctc cgatcgctac gcgctcaccg accctccacc acccagatat
      541 gagcccaacc ccgagctcgg ggtcgatgac caggatcaaa cccagaccga agaaggcggg
      601 tggagcgtga ccgtgacccg cgagatccgc cgtggagagg atgtggtcga cacccaggag
      661 tggggggtcc gctacgcccc ccggttcgct gtcttcgagg tccacccctg caagatcccg
      721 gggaccgcgg tggcctgccc cacgaccacg accaccgcca ccacgaccac ggtgcccacc
      781 acgacttcat catcggcgac tccaccctcg acctagacgg cggacctact cctcttcacc
      841 gtcgtccggg taatgcctca gaaacgagga ggtcacttcc agggacttcg ccaccgattc
      901 ggagtcccaa ccgccgggac ggcgaagcga gatcgtgttc tgttgcacgt agacgtgatc
      961 gatcccaatg tcgagcccga gcagttcggt tgccagcaag ccgggaacgg cgtcacccgg
     1021 tgcatcgccg gtgatcgatt ggccatcttg accggtgaag ctgcggtcgg tgctgaccaa
     1081 caggacgtct cccatggtca cgctgtctct gatggcgacg gtttggccca tgggtccgga
     1141 gatcgtaatc aggagccgcc gaagccacgg ggatggtcgc cgatcacctg gaccttgacc
     1201 aggttggtcg atgcctggat gttgggcggc accccggcga ccatgaccac cgtatcgccg
     1261 gctttggcca atccctcgct ctccagaatc gccgaggcgg cggccatctc gtcgtccgtg
     1321 taatcgcgcc gctcgaattc gtgcggggac acgccccaga agagcgccat gcgacgcaag
     1381 gtggattcgt tggggctgaa ggcgacgatc cgaccttccg gccgatactt cgagatcagc
     1441 cgcgccgtgt tgcccgactc gctgaaggcg acgatcgcgg tcgcccccag gttgatcgcc
     1501 atctc
//
LOCUS       DATITW010000644         1886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_792146, whole genome shotgun sequence.
ACCESSION   DATITW010000644 DATITW010000000
VERSION     DATITW010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792146"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VLS86_08955"
     CDS             <1..330
                     /locus_tag="VLS86_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSO50665.1"
                     /translation="LHVVNVFHLPFTYVDIPVDFVAIAEGHRNAVWGPLEEQLSAAKV
                     YIRRVDLEGYPPDALVSYANDVGASLLVVGTRGRGEVGALILGSTSHRAIHLAACDVL
                     IVKRTAA"
     gene            complement(382..1332)
                     /locus_tag="VLS86_08960"
     CDS             complement(382..1332)
                     /locus_tag="VLS86_08960"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HSO50666.1"
                     /translation="MRRNSQVRRFLAVLAALSLIITACGSDSGDTTSTTAATSTTTLP
                     VTTTQGPEPTTTTTSGATSTTQVESVGHVVYFLLAELDDDDGSPGPFLVPVYREGPST
                     DEAALTALQLLVEGPTPDETAGTPTISTAIPEGTSVNGVTVEAGLATVDLGTEYDDGG
                     GSFGMFARLAQVVYTLTRLPDIDEVSFSIDGEPVTVFSSEGIGLDGPQQREDYYDLLP
                     PIFVDSPAWGEPVTSPIQAKGLSNVFEAVSQIMLTDDDGLPLFEETVMASCGTGCWGE
                     WEVSVPYTVERDQVGALIVWEYSAKDGSRINIREYPILLR"
     gene            1484..1750
                     /locus_tag="VLS86_08965"
     CDS             1484..1750
                     /locus_tag="VLS86_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50667.1"
                     /translation="MGKIIPVGPERTPLPPPPSRITRLQRRARHLVARRLRARPPRLG
                     SGPVTHQEEALLSLMLWGRAIGWVCGALSLLILVIWGLERLLSS"
BASE COUNT          317 a          595 c          614 g          360 t
ORIGIN      
        1 ctccacgttg tcaacgtctt tcacctgcct ttcacctatg tcgacatacc ggtcgacttc
       61 gttgcgattg ccgaaggtca ccgcaacgcg gtctggggcc cgctcgagga gcagctctcc
      121 gcagcgaagg tctacatccg gcgggtcgat ctcgagggct atccgccgga cgctctcgtg
      181 tcgtacgcca acgacgtagg ggcgtctcta ttggtggtgg gtacgcgggg gcgcggcgag
      241 gtgggggcgc tgatcctcgg gagcaccagt caccgtgcga tccatcttgc cgcgtgtgat
      301 gtgctgatcg tcaaacgaac ggcggcatga cggacccgat ccctcgcttg agaaaccgga
      361 cggaggaagc ggcttctgga gctaccggag caggatgggg tactcgcgga tgttgatgcg
      421 gcttccgtcc ttcgccgagt attcccagac gatcaaggcg ccgacctggt cccgctccac
      481 cgtgtaaggc acagaaacct cccactcccc ccagcacccg gtgccacagc tagccatcac
      541 cgtctcttcg aacaacggca gcccgtcgtc gtcggtgagc atgatctggg acacggcttc
      601 gaacacattg ctcaggcctt tggcctggat cgggctggtc accggctcac cccaggccgg
      661 ggagtcgacg aagatcggcg gcaagaggtc gtagtagtcc tcgcgctgct ggggcccgtc
      721 cagcccgatt ccctccgacg agaacaccgt cacgggttct ccatcgatgc tgaacgacac
      781 ctcgtcgatg tcagggagtc gggtcaaggt gtagaccacc tgggcgagac gggcgaacat
      841 cccgaagctg cccccaccgt cgtcgtactc cgtgccgagg tcgactgtgg ccaacccggc
      901 ttcgaccgtg accccgttca cagacgtccc ttcggggatg gcggtggaga tcgtcggggt
      961 tccggcggtc tcgtcggggg tcggcccctc gaccaggagc tggagcgcgg tcagagcggc
     1021 ctcgtcggtg ctcgggccct cccggtagac gggcaccagg aagggtcccg gcgatccgtc
     1081 atcgtcgtcc agctcggcga ggaggaaata cacgacatgt ccgaccgatt cgacctgggt
     1141 ggtcgaagtg gcgccggagg tggtcgtggt cgtgggctcc ggtccctgag tggtggtgac
     1201 cggcaacgtc gtagtggatg tggctgcggt ggtagaggtg gtgtctccac tgtcagagcc
     1261 gcatgcggtg atgatcaggg atagcgccgc aagcaccgcc aggaagcgac gaacctgcga
     1321 attccgtctc atgcctcgat catgcatccg aagccggaac atggacagag tccaacgtca
     1381 cttttcgcgt tgcgggtgaa tgactcctgt cgatcgtggc tcgacgcgtc aggatcgcgt
     1441 caagacttgg cctcttgtcc ccggcatcgt gtatccaggg cacatgggca agatcatccc
     1501 cgttgggcca gagcggaccc cgctcccgcc accgccttcg cggatcaccc gtctccaacg
     1561 cagggcacgc catctcgtgg ccagacgtct tcgagcacgg ccgccgagac tggggagcgg
     1621 cccggtcacc catcaggagg aggccttgct cagcttgatg ctgtggggcc gcgccatagg
     1681 ctgggtgtgt ggcgctctct cgcttctgat tctcgtgatc tggggtctgg agcgcctcct
     1741 ttcgtcatag gtccccatcc gagccggtcg ggtgtcagca actcgtctgg aatcggatga
     1801 tcctcgccaa gaccacgtca gtagcgccga cacatgcccg ggtcggtcgt acaactggag
     1861 cgtgatagat ttccagttcg tgatcg
//
LOCUS       DATITW010000645         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_592447, whole genome shotgun sequence.
ACCESSION   DATITW010000645 DATITW010000000
VERSION     DATITW010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592447"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /gene="rplI"
                     /locus_tag="VLS86_08970"
     CDS             <1..367
                     /gene="rplI"
                     /locus_tag="VLS86_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HSO50668.1"
                     /translation="FLLPKKKAVKAHEGALAQAEAVRLARIESERKAKDDAERIATQL
                     VGSRVVIAAQAGDEGQLYGSIALADVVEGVRRFTGIELDRATVEIPKPIKAIGLHEIN
                     IKLHPEVEFPLTLDVIPAT"
     gene            651..2003
                     /gene="dnaB"
                     /locus_tag="VLS86_08975"
     CDS             651..2003
                     /gene="dnaB"
                     /locus_tag="VLS86_08975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HSO50669.1"
                     /translation="MSGMTEQTQMRPGYRNPPHSREAEESVIGAVLLSEEAVNEVMDR
                     LHPPDFYVPAHQAIFEAMRELFDGNQAIDAVTVSEELRRKGELDKIGGVSYLARLVDI
                     VPSTSNILYYSGIVEEHAKRRELIRAGAAVTDVAFDIDEEIAHVLDRAEQAVLSVAER
                     RSAQTLLEVGPLFSDVLEHMELLEQRGSEMTGLATGFVDIDKKLAGLQKANLVVIAAR
                     PAMGKSSLALGIAVNAATQGEPVAIFSLEMSKEEIVQRILSSVGRVDSMKLRSGQLGP
                     LWQRVVDAAGKMYKTPIYIDDSPVVTVTDIRAKCRRLKRKKGLSLVVIDYLQLMEASN
                     RENRQQEISEITRNLKNLARELDVPVIAVSQLNRSLEAREDKRPRLSDLRESGAIEQD
                     SDVVMFIYRHEYYHPEDQEKKGIAEVIVAKHRAGSTGPVEMTFQPEFTRFANLGRDVT
                     "
BASE COUNT          379 a          690 c          679 g          372 t
ORIGIN      
        1 cttcctgctt cccaagaaga aggcggtgaa ggcacacgaa ggcgccctgg cccaggccga
       61 ggcggttcgc ctcgcccgta tcgagtccga gcgcaaggcc aaggacgatg ccgagcggat
      121 cgcaacccag ctcgtgggga gcagggtggt gatcgcggcc caggccggtg acgaggggca
      181 gctgtacggg tcgatcgccc tggccgacgt cgtcgagggc gtgcgtcgat tcaccggcat
      241 cgagctcgac cgggcgacgg tcgagatccc gaagccgatc aaggccatcg gtctccacga
      301 gatcaacatc aagctccacc ccgaggtgga gttcccactc accctggatg tgattcccgc
      361 cacctgaccg gcacctcctg ggagccaccg ccacgttctt gccgccacgt tcttgggagc
      421 caggggtccg tatctgaccc ctggcggtcc caattacggg tgggtgggcc gccaatagcg
      481 tcgcgtttcg cggtttctcg gagcttttcg cggccatttg cagcagccgc ggcggtcggt
      541 ctatgtttgc cacgcttccc ccgaagctgg tcgatcttcc ccaatctcgt tggtcctgac
      601 gcgccagttc gcgaaatttg tcacacccgg gctgttggct gaggatatcg atgagtggca
      661 tgaccgaaca gacgcagatg aggcccgggt accggaaccc accgcactcc cgggaggcgg
      721 aggagtcggt catcggggct gtgctcctct ccgaggaggc ggtcaacgag gtgatggatc
      781 gtctccaccc gcccgacttc tatgtccccg cccatcaggc catattcgag gcaatgcgcg
      841 agctgttcga cggcaaccag gccatcgacg cggtgacggt gtccgaggag ctgcgaagaa
      901 agggcgagct ggacaagatc ggtggggtct cctatctggc tcgtctggtg gacatcgtcc
      961 cctccacctc caacatcctc tactactccg ggatcgtcga ggagcacgcc aagcgtcgtg
     1021 agctgatccg ggctggggct gcggtcaccg atgtcgcctt cgacatcgac gaggagatcg
     1081 cccacgtgct cgaccgcgcc gagcaggcgg tgctctcggt cgccgagcgc cgcagcgcgc
     1141 agaccctcct cgaggtaggc ccgctgttca gcgacgtcct cgagcacatg gagcttctcg
     1201 agcagcgtgg ctcggagatg accggtctgg cgaccgggtt tgtcgacatc gacaagaagt
     1261 tggccggttt gcagaaggcc aatctggtgg tcatcgccgc caggccggcc atgggcaaat
     1321 cgagcctggc tcttgggatc gcggtcaacg cagcgaccca gggagagccg gtggccatct
     1381 tctccctgga gatgtccaaa gaggagatcg tgcagcggat cctgtcctcg gtggggaggg
     1441 tcgactcgat gaagctccgc tccggtcagc tcggccccct ctggcagcga gtggtcgatg
     1501 ccgcggggaa gatgtacaag acgccgatct acatcgacga ctcaccggtg gtgaccgtga
     1561 ccgacatccg ggccaagtgt cgtcgtctca agcggaagaa gggtctttcc ctggtggtga
     1621 tcgactatct ccagttgatg gaggcctcca accgggagaa ccgccagcag gagatctccg
     1681 agatcacccg taacttgaag aacctggccc gtgagctcga cgtccctgtg atcgccgtgt
     1741 cccagctcaa ccgctccctc gaggccaggg aggacaaacg tccccgtctc tccgacctcc
     1801 gtgagtccgg cgccatcgaa caggacagcg acgtggtcat gttcatctac cggcatgagt
     1861 actaccaccc cgaggaccaa gagaagaagg gaatcgccga ggtgatcgtg gccaagcacc
     1921 gggccggctc caccgggccg gttgagatga cctttcagcc cgagttcacc cgcttcgcca
     1981 acctgggacg cgacgtcacc tagcggtgga gctcccgacc cgcggctaac gcccgctcga
     2041 tcgtctcgaa ggcctccgcc gccggccgcc agccgagggt ggcggtcttc ttgtcctcca
     2101 gctccttgta caccgcgaag
//
LOCUS       DATITW010000646         1583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_151469, whole genome shotgun sequence.
ACCESSION   DATITW010000646 DATITW010000000
VERSION     DATITW010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151469"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VLS86_08980"
     CDS             complement(<1..710)
                     /locus_tag="VLS86_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HSO50670.1"
                     /translation="MRLHLLDGTYELFRAHFGRKESVLASDGSDIRATLGIIESTLAL
                     LREPGVTHLAASFDTVIESFRNEMFAGYKTSAGMDPELLVQFPLAEEALRAIGVVVWG
                     NIEHEADDAIATAAIRWVEDVEQVVIATPDKDLCQLVIGDRIVTYNRREQKILDEDAV
                     VEKFGVHPESIPDYLALVGDTADGVPGLPGWGAKSAATVLFRYPRLELIPANATDWEV
                     TVRSADKLAATLREYVQEV"
     gene            699..1031
                     /locus_tag="VLS86_08985"
     CDS             699..1031
                     /locus_tag="VLS86_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50671.1"
                     /translation="METHDPDPKPAEDVDPWDTVSSEFGSLGDRLKDTYRRVAADRGP
                     SEEEIKGAFATLIGAWDQVAESVTTALRDPEAREHLKKAASSFATALGSTISDLGDEF
                     KGHSDEEE"
     gene            1044..>1583
                     /locus_tag="VLS86_08990"
     CDS             1044..>1583
                     /locus_tag="VLS86_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSO50672.1"
                     /translation="MSHPLISVGGLSERLGEVVLFDLRWSLTEPGQGRVAYATGHIPG
                     ATFVDLETDLSGSSGDGRHPLPTATEFTRLLGALGAGPDDEIVVYDDQEGSVAARMWW
                     MLRSIGHERVRLLDGGLGSWIAAGLPVTDDESGRRPPTHYPNVPGFSGVVTAHGLANR
                     YLIDARAPERYRGDVEPVDP"
BASE COUNT          291 a          527 c          501 g          264 t
ORIGIN      
        1 acttcttgga cgtattcgcg cagcgtcgcc gccagcttgt ccgcgctgcg cacggtcacc
       61 tcccaatctg tggcattggc cgggatcaac tcgagacgcg ggtatcggaa gagaacggtc
      121 gcagccgact tggcgcccca accggggagc ccgggaacgc catcggcggt gtcgccgacc
      181 agggccagat agtcgggaat cgactcgggg tggaccccga acttctccac caccgcatcc
      241 tcgtcgagga tcttctgctc gcgccggttg taggtgacga tccgatcacc gatcaccagc
      301 tggcacaagt ccttgtcggg agtggcgatg accacctgct cgacatcctc cacccaccga
      361 atggcggcgg tggcgatggc gtcgtcggcc tcatgctcga tgttgcccca aaccacgacc
      421 ccgatcgctc tcagggcctc ctcggcgagc gggaactgga ccaacaactc ggggtccatc
      481 cctgccgagg tcttgtatcc ggcgaacatc tcgttgcgaa acgactcgat cacggtgtcg
      541 aagctcgctg cgagatgagt cacccccggc tcccggagca aggcaagggt cgattcgatg
      601 atccccaagg tggccctgat gtcggagcca tcgctggcga gcaccgactc cttgcggccg
      661 aaatgggccc ggaacaactc gtaggtacca tcgaggaggt ggagacgcac gacccagatc
      721 ctaagccggc ggaggacgtc gacccttggg acactgtcag ttccgagttc ggatccctgg
      781 gtgatcggct gaaagacacc taccgccggg tcgccgcaga ccgcggccca tccgaggagg
      841 agatcaaggg agcctttgcg accttgatcg gcgcctggga tcaggtcgcc gaatcggtga
      901 ccaccgcttt gcgcgacccg gaagcgaggg aacatctcaa gaaggcggcc agttccttcg
      961 ccaccgccct cggatcgacc atcagtgatc tcggggacga gttcaaaggg cattccgacg
     1021 aggaggaata gccgattggg accgtgtctc accccctgat ctcggtagga gggctgtcgg
     1081 agcgactcgg cgaggtcgtc ctctttgatc tccgatggtc cctcaccgaa cctggccagg
     1141 ggagagtggc ctatgccacc gggcacatcc cgggcgcgac ctttgtcgat ctcgaaaccg
     1201 acctcagcgg atcatccggt gacggccgcc atcccctgcc aacggcaacc gagttcaccc
     1261 gactcctggg agcgctggga gcgggccccg acgacgagat cgtcgtctac gacgatcagg
     1321 agggctcggt tgccgcccgg atgtggtgga tgctgcgctc catcggccat gagagggtgc
     1381 gtctcctcga cggcggcctg ggttcatgga tcgctgccgg tcttcccgtc accgacgacg
     1441 agagcggccg acgcccgcca acgcactacc cgaacgtccc cggattcagc ggagtggtga
     1501 ccgcacatgg tctggccaac cgctacctga tcgacgcacg agcccccgag cggtatcgcg
     1561 gagacgtcga gccggtcgac ccc
//
LOCUS       DATITW010000647         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_511926, whole genome shotgun sequence.
ACCESSION   DATITW010000647 DATITW010000000
VERSION     DATITW010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511926"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VLS86_08995"
     CDS             <1..763
                     /locus_tag="VLS86_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSO50673.1"
                     /translation="PEQVVAAAEAGKHVLCDKPLAIDVPEARRALAACEAAGVKLGIN
                     FHNRHLPWVKDFTSVIREGVIGDVELVQLQVSSGPRHYENWRADPEIAGLGSVHNVGV
                     HGLDFLRVILGSDPVEVMAMFDKGPETGQVEMLGLILLRFENGALVEYNCNERIRDPL
                     NDIVIYGSSGRIVGTSFTRSRSDGELTILTDLGETVTRYPAPEAHRLSLAAFTHAVLQ
                     GQEPDAAGIDGLRSAQICEAIGRSVRERRLVEVVY"
     gene            772..1842
                     /locus_tag="VLS86_09000"
     CDS             772..1842
                     /locus_tag="VLS86_09000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HSO50674.1"
                     /translation="MTDQVDLSDLKTIDQVIARAREVTEPGAHVWAAAGAGQGVTLTR
                     NALALNKLALVPRVMRDVSEVDLSTSFLGVPLALPVLLAPVGALGLYDPGDALAAATA
                     ATRVGTSAICSTLATSPWEEVAATAPGRHFFQLYVLGGRPWISDVLARVGSAGFAGIC
                     LTVDTPVIGRRDRSLEDGFVWTVPPEGSPNLTQHGMDYSHRSRFTWDDLEWLCGQTAL
                     PVFVKGVMSPADAVESVARGVAGVYVSNHGGRVIDHGLSTIAALEEIVDRVGGEAEVM
                     IDSGFTRGAEVCKALALGARAVGMGRLQCWGLAVGGTSGLVRVLEILRDEVANTMANI
                     GCRSIEEITADHVRWSIPAPPA"
     gene            complement(1847..2887)
                     /locus_tag="VLS86_09005"
     CDS             complement(1847..2887)
                     /locus_tag="VLS86_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HSO50675.1"
                     /translation="MKFPLERLESFVSDVLVALDVLPEHAGTTAQRLLEADLKGRTGH
                     GLIRLPQYVPRIEAGGFNLRPEIQLRRETAVSALVDGDNGLGQVVMTRAVETAIDKAI
                     ESGIAWVGTVHSNHAGAAGVYTSMALRHDLIGVYLAVASANVMPPWGGTERLLGTNPI
                     SIAIPAGEETPFLLDIATTVTSHGTIKVVAQAGEMMPEGWVVDLEGNPILDPHRADEG
                     FLVPIGGYKGSGLNMAIGLLAGVLNDAAFGDEVIDHRKVEGQGANTGQAVLVMRPDLF
                     RDLDAFKTAMDHHLRSLRAAGDPGSVLIPGETAARLEDEQRTGGIEVPEALLGQLREL
                     GSRFGLEDRLDD"
     gene            complement(2898..3281)
                     /locus_tag="VLS86_09010"
     CDS             complement(2898..3281)
                     /locus_tag="VLS86_09010"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSO50676.1"
                     /translation="MAELIRFDEAEPVARGDGIESIRLTPSPLDTQGFVMGITSFPPG
                     TSIRLHSHNTIEQVTVIEGEGLAELNGEQLPAHPYDTTQIAPGEWHRFINTGPTVMRI
                     LWVYGSTDVTRTFADTGETVEQFAR"
     gene            complement(3300..>3503)
                     /locus_tag="VLS86_09015"
     CDS             complement(3300..>3503)
                     /locus_tag="VLS86_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSO50677.1"
                     /translation="LAPSEVLTERIEQLRDKAALEATAASIPLGRYQSPEELAQWVLF
                     LASPEAGFMTGQTVSVNGGRFMA"
BASE COUNT          567 a         1167 c         1181 g          588 t
ORIGIN      
        1 tcccgagcaa gtggtggctg ccgccgaagc cggcaagcac gtcctttgcg acaaacccct
       61 ggccatcgac gtccccgagg cgagacgggc cctcgcggcc tgtgaggcag cgggcgtcaa
      121 gctcgggatc aacttccaca accggcatct gccctgggtc aaggacttca ccagcgtgat
      181 ccgagaaggg gtgatcggcg acgtcgagtt ggtccagctc caggtgagct ccggcccccg
      241 ccactacgag aactggcggg cggaccccga gatcgccggt ctcggctcgg tgcacaacgt
      301 cggagttcac gggctcgact tcttgagggt gatcctcggc tccgatccag tcgaagtgat
      361 ggcgatgttc gacaagggcc cggaaaccgg tcaggtcgag atgctcggct tgatcctgct
      421 ccgcttcgag aacggggcgc tcgtcgagta caactgcaac gagaggatcc gcgacccgct
      481 caatgacatc gtcatctatg gatcgtcggg gcggatcgtg gggaccagct tcacccggtc
      541 gcgaagcgac ggcgagctga ccatcctcac cgatctcggc gagaccgtga ctcgataccc
      601 cgcaccggaa gcacatcggc tgtctctggc cgcgttcacc catgccgtgc tccaggggca
      661 agagccggac gcggcgggca tcgacgggct gcgcagcgcc cagatctgtg aggcgatcgg
      721 gcgctcggtg cgagagcgac gtctggtaga agtggtctac tgatctccgg cgtgacagac
      781 caagtcgacc tatccgacct gaagacgatc gatcaggtga tcgcccgcgc ccgagaggtg
      841 accgaaccgg gcgcacacgt ctgggctgcg gccggagccg gccagggagt cacgttgacc
      901 cggaatgcgc tggcactcaa caagctggcc ctcgtcccca gggtcatgcg cgatgtcagc
      961 gaggtggacc tctccacctc gttcctgggg gtgccgctcg ccctgcccgt cctgctggcg
     1021 ccggtgggcg ctctcgggct ctacgacccg ggagatgccc tggccgcggc gacagcagcc
     1081 acccgggtcg gcacctctgc catctgctcg accctcgcca cctctccctg ggaggaagtg
     1141 gcagccactg cccccggtcg ccatttcttc cagctatacg tgcttggcgg caggccatgg
     1201 atcagtgacg tcctggcccg ggtggggagc gccggcttcg ccgggatttg cctgaccgtc
     1261 gacaccccgg tgatcgggag acgggatcgg agcctggaag acggcttcgt ctggaccgtg
     1321 ccccccgagg gatcgccaaa cctgacccag cacggcatgg actactcgca ccggtcgagg
     1381 ttcacctggg acgacctgga gtggctctgt gggcagacgg ccctgcccgt gttcgtgaag
     1441 ggggtgatga gcccggctga cgccgtcgag tcggtcgccc gcggcgtcgc cggtgtctac
     1501 gtgtccaatc acgggggcag ggtcatcgac cacggtctga gcacgatcgc agcgctggaa
     1561 gagatcgtcg accgggtcgg cggcgaggcg gaggtgatga tcgacagtgg cttcacccga
     1621 ggggcggagg tgtgcaaggc gctcgccctt ggggcccgtg cggtgggcat ggggcgactg
     1681 cagtgctggg gcctggcggt tggcggcaca tccgggctgg tccgggtgct ggagatcctc
     1741 cgcgacgagg tcgccaacac catggccaac atcggctgtc ggagcatcga ggagatcacg
     1801 gccgaccatg tgagatggtc gatccccgct cccccggcct gatgacctag tcgtcgagtc
     1861 ggtcctcgag gccaaatctg gagccgagct ccctcaattg cccgagcagg gcctcaggca
     1921 cctcgatgcc gccggtgcgc tgctcgtctt ccagtcgggc ggcggtctca cccgggatca
     1981 ggacgctccc gggatcgccc gcggcccgca gcgagcgcag atggtggtcc atggctgtct
     2041 tgaacgcatc gaggtcacgg aacaggtcgg gtcgcatcac gagcacggcc tgaccggtgt
     2101 tggccccctg gccctcgacc ttccggtggt cgatcacctc gtccccgaag gcggcgtcgt
     2161 tgagcacccc ggcgagcagc ccgatcgcca tgttcagacc cgatcccttg tacccaccga
     2221 tcggcacgag aaacccctcg tcggcgcggt gagggtcgag gatcgggttc ccctcgagat
     2281 cgaccaccca gccctcgggc atcatctcgc ccgcctgggc gacgaccttg atcgttccgt
     2341 gtgaagtgac cgtggtggcg atgtcgagca ggaacggcgt ctcctccccg gccgggatcg
     2401 cgatcgagat cgggttcgtg ccgagcagac gttcggtgcc accccacggg ggcatcacat
     2461 tggcgctggc cacggcgaga tagacgccga tcagatcgtg acggagggcc atcgaggtgt
     2521 acacccccgc agcaccggcg tggttggagt gaacggtgcc cacccaggcg attccagact
     2581 cgatcgcctt gtcgatcgcg gtctccacgg ctctggtcat caccacctgt cccagcccgt
     2641 tatcaccatc gaccagggcg ctgaccgcgg tctcccgacg cagctggatc tccgggcgca
     2701 ggttgaagcc ccccgcctcg atccggggca cgtattgggg cagccggatc aagccgtgcc
     2761 cggtgcggcc cttgaggtcg gcttcgagaa gccgctgcgc ggtcgtcccg gcatgttcgg
     2821 gcaggacgtc gagggcgacc aggacgtcgg agacaaacga ctccagacgc tcgagcggga
     2881 acttcaccgg ggggacttca ccgggcgaac tgctcgaccg tctcaccggt gtcggcgaag
     2941 gtccgggtca cgtcggtgct cccatagacc cagaggatcc tcatcaccgt gggcccggtg
     3001 ttgatgaacc tgtgccattc gccaggcgcg atctgggtgg tgtcataggg gtgggccggc
     3061 agctgctctc cgttcagctc ggccaacccc tcgccctcga tgacggtcac ctgctcgatc
     3121 gtgttgtggc tgtggagacg aatgctggtg ccggggggga agctggtgat gcccatcacg
     3181 aagccctggg tgtcaagcgg gctgggagtg agacggatgc tctcgatccc gtcaccccgg
     3241 gccaccggct ccgcctcgtc gaatctgatc agctcagcca aggacatcct ccgctccggt
     3301 catgccatga acctaccgcc gttgaccgac acggtctgac cggtcatgaa tcccgcttcc
     3361 ggcgaggcca ggaacaagac ccattgcgcc agttcctccg ggctctgata tctgcccaac
     3421 gggatcgagg ccgccgtcgc ctcgagtgcc gccttgtccc gaagctgctc gatgcgctcg
     3481 gtgagcacct cagacggggc gag
//
LOCUS       DATITW010000648         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_792296, whole genome shotgun sequence.
ACCESSION   DATITW010000648 DATITW010000000
VERSION     DATITW010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792296"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VLS86_09020"
     CDS             <1..557
                     /locus_tag="VLS86_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSO50678.1"
                     /translation="PTEESVRRALAIQLIINKELGLSKNENPLQGSFIIEELTDLVEE
                     AVLAEFERLNSRGGVLGAMERQYQRTKIQEESLYYEQQKESGAYPVVGVNTFISKTGS
                     PFVIPDELIRSTDEEKDRQIANVRAFQQRNRVDAEAALAALQKTAISGENIFAQLMET
                     AKVASLGQMSNALYQVGGQYRRNM"
     gene            complement(611..1390)
                     /locus_tag="VLS86_09025"
     CDS             complement(611..1390)
                     /locus_tag="VLS86_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HSO50679.1"
                     /translation="MSTPPPSPSGARLAIRIVVGGLIVVIGAVALLNVFTSANMEEAT
                     DSYPGIAALDLDLRNGPVVVVAGETDEVVVEKSYTTGFLGGTTEAEVDGDTLRLIQRC
                     PAIFGFGCRSSYRVTVPAATTVTGRTSNGAIRIDGLDGAIDVATSNGAVDLERLTSTV
                     TARTSNGAVSGTGLLSSDLDVSTSNGRVTLEFAQAPSSVRVTTSNGEIDVTLPEDSPP
                     YAVSTSTSNGRVSNEIRTDPAAGETIDLRTSNGDISLRYPG"
     gene            1477..>2091
                     /locus_tag="VLS86_09030"
     CDS             1477..>2091
                     /locus_tag="VLS86_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSO50680.1"
                     /translation="MEAIMDAETIQRRRWLTLVVLSLSLVIIGLDNTILNVALPTLVR
                     ELGASASELQWMVDSYVLVFAGLLLTMGALGDRFGRKLALNIGLVVFGLSSIAAALAD
                     SADALIIARALMGVGGALIMPSTLSIITNIFTGAERGRAIAAWAAVAGLGIILGPVIG
                     GWLLESFWWGSIFLINVFIVVVAIVAGWFLVPESKDPAATPLDPL"
BASE COUNT          371 a          644 c          691 g          385 t
ORIGIN      
        1 ctccaaccga ggagtcggtc cgtcgcgccc tcgccattca gctaatcatc aacaaggagc
       61 tgggtctgtc caagaacgag aacccgctcc agggcagctt catcatcgag gaattgaccg
      121 atctggtcga ggaggcagtg ctcgccgagt tcgagcggct caacagccgc ggcggagtgc
      181 tcggagcgat ggaacgtcag tatcagcgga cgaagatcca ggaagaatcc ctctactacg
      241 agcagcagaa ggaatcaggc gcctatccgg tggtcggcgt caacaccttc atctccaaga
      301 ccggctcgcc tttcgtcatc cccgacgagc tgatccggtc gaccgacgag gagaaggacc
      361 gccagatcgc caatgtgcgc gcatttcagc agcgaaaccg ggtggacgca gaggccgcgc
      421 tcgccgccct gcagaagacg gcgatctccg gtgagaacat cttcgcccag ctgatggaga
      481 cagccaaagt ggcctcgttg gggcagatga gcaatgccct gtaccaggtc ggcggtcagt
      541 atcgacgcaa catgtagccc tgtgccgcgg gcgggcgagt ggtaacaact gtggtgggca
      601 gggccgcggg tcagccggga taacggaggc tgatgtcgcc gttcgaggtg cggagatcga
      661 tagtctcccc ggcagccgga tccgtcctga tctcgttgct tactcgccca ttggacgtcg
      721 acgtgctcac tgcgtacggc ggcgagtcct cggggagggt gacatcgatc tcgccgttgc
      781 tggtggtcac ccgcaccgat gacggggcct gggcgaattc gagggtcacc cggccgttgc
      841 tggtggaaac gtcgaggtcg gaagagagaa gaccggtgcc gctcacggcg ccgttcgagg
      901 tacgggcggt gaccgtggag gtgagacgct ccagatcgac cgccccgttg ctggtggcga
      961 catcgatggc gccatcgagg ccatcgatcc tgatcgcgcc gttggatgtc ctgccggtga
     1021 cagtcgtcgc cgcgggcacc gtgacccggt atgagctccg gcagccaaaa ccgaagatcg
     1081 ccgggcagcg ctggatcagg cgaagcgtgt caccgtccac ctcggcctcg gtggttccgc
     1141 cgaggaatcc cgtggtgtag ctcttctcca ccaccacctc atcggtctct ccggccacaa
     1201 cgaccaccgg cccgtttctc aaatccagat cgagcgcggc aatgcccgga tagctgtcgg
     1261 tggcctcttc catgttggcg ctggtgaaga cattgagaag tgccaccgca ccgatgacca
     1321 cgatgagccc cccgaccacg atccggatcg cgaggcgggc acctgacgga ctgggtgggg
     1381 gagtggacac ggatggaacc atacgccggg ccgctatagc tccgccttgg cacgagcggt
     1441 ggcttccgat acgataccgt accgtatcgt ttcgtaatgg aggcaatcat ggatgcggag
     1501 acgattcagc gcagaagatg gctgacgctg gtggttctgt cattgagcct ggtgatcatc
     1561 gggctcgaca acacgatcct caacgtcgcc ctccccaccc tggtccggga gctcggggca
     1621 tcggcgagcg agttgcagtg gatggtcgat tcctatgtcc tggtcttcgc cgggctgttg
     1681 ctgaccatgg gtgccctcgg cgaccgcttt ggccgcaagc tcgcgctcaa catcggtctg
     1741 gtggtgttcg gcctcagctc gattgccgcg gccctggccg acagtgccga cgccctgatc
     1801 atcgcccgag ccctgatggg ggtgggggga gcgctgatca tgccgtccac gctctccatc
     1861 atcaccaata tcttcaccgg cgccgagaga gggagggcga ttgccgcctg ggccgcggtc
     1921 gccggtctcg ggatcatcct gggcccggtg atcgggggat ggctgctgga gagcttctgg
     1981 tggggatcga tcttcctgat caatgtgttc atcgtcgttg tggcgatcgt cgccggctgg
     2041 ttcctggtcc cggagtcgaa ggaccccgca gcgacgcctc tcgacccgct t
//
LOCUS       DATITW010000649         1560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_391885, whole genome shotgun sequence.
ACCESSION   DATITW010000649 DATITW010000000
VERSION     DATITW010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391885"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VLS86_09035"
     CDS             <1..1005
                     /locus_tag="VLS86_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSO50681.1"
                     /translation="CYGISLATGKTVETGEAVGIMAAQSIGEPGTQLTMRTFHTGGVA
                     GEDITHGLPRVVELFEARAPKGKAVMAEEKGVITVVEDEQGRRILLEAGDDELIYPIS
                     RYAKLRPGVSSGVTIDAGTQLTEGPLDPKEGLEIRGVRATQQYLVDQVQEVYRSQGVE
                     IHDKHIEMIVRQMLRRVRVVSPNESDFLPAALVDDQTFRDTNRELVTDGKKPAEGRPE
                     LMGITKASLATDSWLSAASFQETTRVLTDAAIQGRSDFMRGLKENVIIGKLIPAGTGA
                     QQYQMLQPSLPGATTVASLGFLDGGPTSSGDDGLPEDPAEWLASLGGSIRPDDEAEEE
                     "
     gene            1059..>1560
                     /locus_tag="VLS86_09040"
     CDS             1059..>1560
                     /locus_tag="VLS86_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50682.1"
                     /translation="MTPISLPRSFPWSRDWGRRDPIPTSRYPDDGNQQPPGPRRSRRL
                     GPEELARDLANRLKATRDPDADGRRFLPHFDMLNAGRLILGLGALLILAGLVAGFTWL
                     SGLAGYQTTGMVIETSPVYGCPGEPELGKAFAGESITLIGRSPDQVWLAVRDRRGPGD
                     TVYVLAS"
BASE COUNT          289 a          494 c          509 g          268 t
ORIGIN      
        1 tgttacggga tctcactggc caccggcaag acggtggaaa ccggtgaggc ggtggggatc
       61 atggccgccc agtcgatcgg cgagccggga acccagctca ccatgagaac cttccacacc
      121 ggaggagttg ccggcgagga catcactcac ggtcttcccc gcgtcgtcga gctctttgag
      181 gctcgggcac ccaaggggaa ggcagtcatg gccgaggaga agggtgtgat caccgtcgtc
      241 gaggacgagc aaggccgtcg catcctgctc gaggcaggcg atgacgagct catctacccc
      301 atctcccgct atgcgaagct gagaccgggc gtctccagcg gcgtgaccat cgatgccggc
      361 acccagctca ccgagggacc gttggacccc aaggagggtc tcgagatccg cggagtgcgg
      421 gccacccagc agtacctggt cgaccaggtc caagaggtat atcgctccca gggtgtcgag
      481 atccatgaca agcacatcga gatgatcgtg cgtcagatgc tccgccgggt gagggtggtc
      541 tctcccaacg agtccgactt cctgcctgcg gccctggtcg acgatcagac cttccgtgac
      601 accaaccggg agttggtcac cgacggaaag aagccggccg agggcagacc cgagctgatg
      661 gggatcacca aggcgtcgct tgccacggat tcgtggcttt ctgctgcctc gttccaggaa
      721 acgacccggg tcctcaccga cgccgcgatc cagggacggt ccgacttcat gagaggcctc
      781 aaggagaacg tcatcatcgg caagctgatc ccggcaggca ccggcgccca gcagtatcaa
      841 atgctgcagc cgtcgcttcc cggtgcgacc acggttgcca gtctcggatt cctcgacggg
      901 ggcccgacgt cgtccggtga cgacggcctt cccgaggacc ccgccgaatg gctggccagt
      961 cttggtggca gcatccgccc cgacgacgaa gccgaagagg aatagtcgtc gaccgcgaac
     1021 gtgtgtgaag gggccgtcat cctgcggccc cttcgacgtt gacaccaatc tcactgccac
     1081 gtagctttcc ctggtcgcgg gactggggac ggagagatcc cattccgaca tccagatatc
     1141 ccgatgacgg caaccaacaa ccaccagggc cgcgtaggtc gagacgtctc ggccccgagg
     1201 agctggctcg tgacctggcc aatcggctga aggcaacccg tgatccggat gccgacgggc
     1261 gacggtttct tcctcatttc gacatgctca atgcgggtcg gctcatcctc ggtctgggcg
     1321 cactcttgat ccttgctggt ctcgtggccg ggttcacctg gctcagcggg ctggccgggt
     1381 accagacgac gggaatggtg atcgagacgt cgcctgtata cggatgtcct ggtgagccgg
     1441 agctggggaa ggcgttcgca ggtgagtcca tcaccttgat tggccgcagc cccgaccagg
     1501 tctggctcgc agtacgcgac cggcgcgggc cgggtgacac cgtgtatgtg ttggcgtcgg
//
LOCUS       DATITW010000650         1573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_351703, whole genome shotgun sequence.
ACCESSION   DATITW010000650 DATITW010000000
VERSION     DATITW010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351703"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /gene="purH"
                     /locus_tag="VLS86_09045"
     CDS             <1..1192
                     /gene="purH"
                     /locus_tag="VLS86_09045"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HSO50683.1"
                     /translation="SLYPFRATVAEPSASEEEIIEKIDIGGPTMIRAAAKNHAHVGVV
                     TSQAQYETVATAVESGGLPDELRRALAAEAFFHTAAYDAAIVGWIGDDLVIPLRRVSE
                     LRYGENPHQPASLFVEEGASPWWVRATLHQGKEMSFNNYADAEAAWRLATGQPGCVAI
                     IKHTNACGAAYGDTVLETFEAAWECDPVSAFGGVVAINGVLDEQTAEAITGRFVEVVV
                     CAGITEGAKQILAGRGNLRVLEAGPPGASDPDLRRLESGLLAQARDSDQAETWEVVSD
                     RQPTEGEMADLEFASKVAMHAKSNAVVVVNDRAAVGVGAGDQSRVGAAERAVIRAGAR
                     SKGAVAASDAFFPFRDGLDVLAGAGVTAVVEPGGSRNDPDLIAAANEHGLALVFSGRR
                     HFKH"
     gene            1192..>1573
                     /locus_tag="VLS86_09050"
     CDS             1192..>1573
                     /locus_tag="VLS86_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HSO50684.1"
                     /translation="MSAQILDGKAVAATVKAEVARAVASLGYAPGLATVLVGDDPASH
                     TYVRGKRTDAEEVGMVSIHHQLAASVPQGELEALIDMLNADQAVDGILVQLPLPGDLD
                     SERIVARIDPAKDVDGLHPYNLGLL"
BASE COUNT          259 a          515 c          557 g          242 t
ORIGIN      
        1 cagtctctac cctttccggg ccacggtcgc cgagccctcg gcctccgagg aggagatcat
       61 cgagaagatc gacatcggtg gcccgacgat gatccgggcc gcggccaaga accacgccca
      121 tgtgggcgtg gtcacctcac aagcccagta cgagacggtg gccacggcgg tggagagcgg
      181 ggggctcccc gacgagctgc gacgggccct cgccgccgag gcgttctttc acacggccgc
      241 ctacgacgct gcgatcgtcg gctggatcgg cgacgacctg gtcatcccgc tgcggagggt
      301 gtccgagctg cgctacgggg agaatccgca ccagcctgcc tcgctgttcg tcgaggaagg
      361 cgcctcgccc tggtgggtcc gggcgacctt gcaccaggga aaggagatgt ccttcaacaa
      421 ttacgccgac gccgaggcgg cgtggaggct cgccaccggg cagccgggtt gtgtggccat
      481 catcaagcac accaatgcct gcggcgccgc ctacggggac accgtcctcg agaccttcga
      541 ggctgcatgg gagtgtgacc cggtttccgc ctttggcgga gtggtcgcga tcaacggcgt
      601 gctcgacgag cagacggcgg aggcgatcac cggcaggttc gtcgaggtgg tggtatgcgc
      661 cggaatcacc gaaggggcga agcagatcct ggccggcagg ggcaatctcc gggtcctgga
      721 agcggggcct ccaggggcat ccgatcccga tctgcggcga ctcgagtcgg gcctgttggc
      781 ccaggcccgc gactctgacc aagcggagac ctgggaagtc gtttccgacc gtcagccgac
      841 ggagggcgag atggctgacc tcgagttcgc ctccaaggtg gcgatgcacg ccaaatcgaa
      901 cgccgtcgtc gtcgtcaacg accgtgccgc ggtcggagtc ggggccgggg atcaatcgcg
      961 cgtcggagcg gccgagcggg ccgtgatccg ggccggtgcg aggtcgaagg gcgccgtcgc
     1021 cgcctctgat gccttcttcc catttcgcga cggtctggac gtcctggcag gagcgggggt
     1081 caccgcagtc gtcgagcccg ggggctcacg aaacgacccc gatctgatcg cagctgccaa
     1141 cgagcacggg ttggccctgg tcttctccgg cagaaggcac ttcaagcact gatgagcgcc
     1201 cagatcctcg acggaaaggc ggttgccgcg acggtcaagg ccgaggtggc cagggccgtt
     1261 gcctcgctcg gctacgcgcc gggtctggcc acggtcctgg tcggagacga tccggcgtcc
     1321 cacacctatg tccggggcaa gaggaccgac gccgaggagg tggggatggt ttcgatccac
     1381 caccagttgg cggcgtcggt cccccagggc gagttggaag cgctgatcga catgctcaac
     1441 gccgaccagg ccgtggacgg gatcctggtc caactccccc tgcccggcga cctggactcc
     1501 gagcgaatcg tggcccggat cgacccggcc aaggatgtcg atggcctgca cccatacaat
     1561 ctcgggctgc tcg
//
LOCUS       DATITW010000651         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_191510, whole genome shotgun sequence.
ACCESSION   DATITW010000651 DATITW010000000
VERSION     DATITW010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191510"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VLS86_09055"
     CDS             complement(<1..791)
                     /locus_tag="VLS86_09055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885297.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HSO50685.1"
                     /translation="MSDLTITADQITKSLREQLSGWEPEVEKETVGYVTSIADGVARV
                     DGLPGVMASELLEFPGGLLGVTLNIDEDDLGVVVLGEASHIQQGDPVKQTGTVLSVPV
                     GDALLGRVIDPLGNPVDGKGPIDTTERRLMETQAPSVVQRQPVNEPLQTGIKAIDAMT
                     PIGRGQRQLIIGDRQTGKTAILVDTIINQKVNQGTKDEVKCIYVAIGQKASTVAEVVA
                     TLEEAGAMEYTVVVNAAASAAPALQMYAPYAGSAIGQHWMYNGQHA"
     gene            complement(804..1337)
                     /gene="atpH"
                     /locus_tag="VLS86_09060"
     CDS             complement(804..1337)
                     /gene="atpH"
                     /locus_tag="VLS86_09060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HSO50686.1"
                     /translation="MDDRVRGYARAILDLATAEGELERVEAEFVAVGRAIEKSEELRS
                     SLIDPRLPLDKKHAIIDDLIGGRASSLTVGLVQFIAAQGRASEIPAMAKAFIEQAVES
                     RDSAVAEVRSAVPLDDQTIERLAAALGKATGKKVEVKVVVDESVIGGIVARVGDVVID
                     GSLAHAIGELRQAVLSR"
     gene            complement(1338..1892)
                     /gene="atpF"
                     /locus_tag="VLS86_09065"
     CDS             complement(1338..1892)
                     /gene="atpF"
                     /locus_tag="VLS86_09065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HSO50687.1"
                     /translation="MHTLNTVLILATEEGEETSGGLSLVLPHTSELIAGILAFAIVFF
                     FAWKWALPTINKTLEARQEAITGQLAHAEETKKEAESLLADYKAQLADAKAEGNRLIE
                     EARMAGDQLKADIVSRAEEQAAQTLAKAREEAQAEKARAMSEARREVGEISVELAERI
                     VGGSLDDKMQKDLIERYLAELETL"
     gene            complement(1892..2122)
                     /gene="atpE"
                     /locus_tag="VLS86_09070"
     CDS             complement(1892..2122)
                     /gene="atpE"
                     /locus_tag="VLS86_09070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HSO50688.1"
                     /translation="MEGNIEALTGALALVGYGLGAIGPGIGIGIVAGNAITAMARQPE
                     MQGTIRTTMFLGIALTEALALIGFVLFFIASA"
     gene            complement(2134..2937)
                     /gene="atpB"
                     /locus_tag="VLS86_09075"
     CDS             complement(2134..2937)
                     /gene="atpB"
                     /locus_tag="VLS86_09075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HSO50689.1"
                     /translation="MILATECDVANEVICAPADVTELFEFPTIFTIGGMEFSRTEILM
                     FLAAFITVALLYFGLRGQRLVPTKFGAAVEGMVGFVRDGIARDVIGPEGTKYLPYLMA
                     MFSFLLVGNLFEVTPLINFPITSRMAIPAFLALVTYVIFMFMGFARNNFRYLIDTAWP
                     KSVPIGLRWLVGLIELVSVFLLRPITLAVRLFANMVAGHLMLTLLLGSGVIFIAAIPE
                     IGVKGTIGVFWFALGLFMFLLEILVAVLQAYIFTLLTAIYIESSIHFEH"
     gene            complement(2934..3347)
                     /locus_tag="VLS86_09080"
     CDS             complement(2934..3347)
                     /locus_tag="VLS86_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50690.1"
                     /translation="MDLEHGPPIEANIARSTVARAVYVAPVLVLLFGALRGWEGAWSA
                     AIGVGVVALNFLLAGAILSISARISLQAYHAAALIGFFLRLVLFIGAVYLIASLVEVD
                     RVAFGISAVVAYFTLLTWEAIAMSKEREREPSWRR"
     gene            complement(3337..3573)
                     /locus_tag="VLS86_09085"
     CDS             complement(3337..3573)
                     /locus_tag="VLS86_09085"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HSO50691.1"
                     /translation="MKDETTHQHVGHAISEGWIQGGAFSGSILSGALLGYLADMWLGT
                     EPWLVVIGIVVGSYSGFLNMWRYSKKTEEVGRGS"
BASE COUNT          688 a         1216 c         1212 g          666 t
ORIGIN      
        1 gcgtgctggc cattgtacat ccagtgctgg ccgatggccg atccggcgta gggggcgtac
       61 atctggagcg caggcgccgc cgaggccgcc gcgttgacga ccaccgtgta ctccatggcg
      121 ccggcctctt cgagggtggc aaccacctcc gccacggtgg aggccttctg gccgatggcg
      181 acgtagatgc acttgacctc gtccttggtg ccctggttga ccttctggtt gatgatggtg
      241 tcgaccagga tcgcggtctt gccggtctgc cggtcaccga ttatcagctg gcgctggccg
      301 cgcccgatgg gggtcatggc gtcgatcgcc ttgatcccgg tctgcagcgg ctcgttcacc
      361 ggctgccgtt ggaccaccga cggggcctga gtctccatga ggcggcgctc ggtggtgtcg
      421 atcgggccct tgccgtcgac cgggttgccc agagggtcga tgacccgccc cagcaaggcg
      481 tcgcccaccg gcaccgagag caccgttccc gtctgcttaa ccgggtcgcc ctgttggatg
      541 tggctggcct caccgaggac caccacgccc aggtcgtcct cgtcgatgtt gagggtgaca
      601 cccagcaggc cgccggggaa ctcgagcagc tccgatgcca tcaccccggg aagcccgtcg
      661 acgcgagcca ccccgtcggc gatcgaggtc acatagccga cggtctcctt ctcgacttcg
      721 ggctcccatc cggagagctg ttcccgaagg gacttggtga tctgatcggc ggtaatggtc
      781 aagtcggaca tgggtaataa ctcctagcgg ctcagtacgg cttggcgcag ctcaccgatg
      841 gcatgggcta gcgatccgtc gatgacgacg tcgccaactc gggcgacgat cccgccgatg
      901 accgattcat cgacgacgac cttcacttcg accttcttgc cggtggcctt gcccagggct
      961 gcggcgaggc gctcgatggt ctggtcgtcg agcggcacgg ccgagcgcac ctcggcaacg
     1021 gcgctgtccc gggattcgac ggcctgctcg atgaatgcct tggccatcgc cggtatctcc
     1081 gacgcccgac cctgggcggc aatgaactgg accaggccca cggtgagcga cgaagcccgg
     1141 ccaccgatca ggtcgtcgat gatggcgtgc ttcttgtcca gagggaggcg ggggtcgatg
     1201 agagaagacc tcaactcctc ggacttctcg atcgcccggc cgacggcgac gaactcggcc
     1261 tccacccgct ccagctcacc ctcggcggtg gcgagatcga ggatcgcccg tgcgtaaccg
     1321 cggacccggt cgtccatcta cagcgtctcc aactcggcca gatagcgctc gatcaggtcc
     1381 ttctgcatct tgtcgtcgag ggatccgccg acgatccgct ccgcgagctc gaccgagatc
     1441 tcgcccactt cgcggcgcgc ttcgctcatg gcacgtgcct tctcggcctg tgcctcctca
     1501 cgagccttgg ccagggtctg cgccgcctgc tcctccgctc gggagacgat gtccgccttc
     1561 aactggtccc cggccatccg ggcctcttcg atgaggcgat tcccctccgc cttggcgtcg
     1621 gcgagctgag ccttgtagtc ggcgagaagg ctctcggcct ccttcttggt ctcctcggcg
     1681 tgggccagct ggccggtgat cgcctcctgg cgggcctcca gcgtcttgtt gatcgtgggg
     1741 agggcccatt tccaggcgaa gaagaagacg atggcaaagg cgaggatccc cgcgatcaat
     1801 tccgacgtat ggggcaggac caggctgaga ccgcctgagg tctcttcccc ttcctcggtg
     1861 gccagaatca ggaccgtgtt caaggtgtgc atcacgcgct agcgatgaag aagaggacga
     1921 atccgatcag cgccagcgcc tcggtgaggg cgatgccgag gaacatggtg gtccggatgg
     1981 ttccctgcat ctcgggctgg cgagccatgg cggtgatcgc gttcccggcg acgatgccga
     2041 tgccgatgcc ggggccgatg gccccaaggc catagccgac cagggccagt gcgccggtga
     2101 gggcctcgat gttgccttcc attctctttc tccttagtgc tcgaagtgga tggacgattc
     2161 gatgtaaatc gcggtgagca gggtgaagat gtacgcctgg aggaccgcca ccaagatctc
     2221 caggaggaac atgaacaggc ccagagcgaa ccagaacaca ccgatggtgc ccttgacccc
     2281 gatctcgggg atggcggcga tgaagatcac acccgatccc aggagcaggg tgagcatcag
     2341 gtgaccggcc accatgttgg cgaagagccg gacagccagc gtgatcgggc ggagcaagaa
     2401 gaccgagacc agctcgatca ggcccaccag ccatctgagg ccgattggga ccgacttggg
     2461 ccaggcagta tcgatcaagt atcggaagtt gttccgggcg aatcccatga acatgaagat
     2521 gacataggtc accagggcga ggaacgccgg gatcgccatc cgggaggtga tcgggaagtt
     2581 gatcaacggg gtgacctcga acagattgcc caccaggagg aagctgaaca tcgccatcag
     2641 gtaggggagg tatttcgtcc cctcggggcc gatcacgtca cgggcgatcc cgtcgcggac
     2701 gaacccgacc atcccctcca ccgcggcgcc gaacttggtc gggaccaatc gctggcccct
     2761 gaggccgaag tagaggaggg ccaccgtgat gaaggcggcg aggaacatca ggatctcggt
     2821 gcgactgaac tccatcccgc cgatggtgaa gatggtcggg aactcgaata gctcggtgac
     2881 gtcggccgga gcgcatatca cctcgttggc gacgtcacat tcggtggcga ggatcatctg
     2941 cgccaactgg gttccctttc tctctccttg ctcatcgcta tcgcttccca cgtcagcagg
     3001 gtgaaatacg ccaccacagc cgagatccca aacgccacac ggtccacctc gaccaggctg
     3061 gcgatgagat acaccgcgcc gatgaacaga acgagacgaa ggaagaagcc gatcagagcg
     3121 gcggcatgat aagcctggag gctgatcctt gccgaaatcg agaggatggc cccggccagc
     3181 aggaagttga gggcgacaac tccgactccg atcgcggcgc tccacgcccc ttcccatccc
     3241 ctgagcgccc cgaacaggag aacgaggacc ggagcgacgt agacggctcg cgccacagtg
     3301 ctgcgcgcga tgttggcctc gatcggcgga ccgtgctcaa gatccacgtc ccacctcctc
     3361 ggtcttcttc gagtagcgcc acatgttcaa gaaccccgaa taggagccga cgacgattcc
     3421 gatgaccacc agccacggct cggtgccgag ccacatgtcg gcgaggtatc cgaggagggc
     3481 gccggagaga atcgagccgc tgaacgcgcc gccctgaatc cagccttcgc tgatggcgtg
     3541 tccgacatgt tgatgagtgg tctcgtcctt caccggtggt caccgccatc cgtgggcgct
     3601 tgtgaatcta ttcacaaagt ttagcgaccc gtagctcaca ggcgaaatcg acgctagcag
     3661 tctgatagtg gctcacttcg tgcgccttgg gggtaggtca tcagacatgc cccgcgaagt
     3721 cgtgggggta cgggggaaag ctcgcctcca gctcggcgac cccgcccgct acctcggcga
     3781 gg
//
LOCUS       DATITW010000652         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_351889, whole genome shotgun sequence.
ACCESSION   DATITW010000652 DATITW010000000
VERSION     DATITW010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351889"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VLS86_09090"
     CDS             <1..450
                     /locus_tag="VLS86_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50692.1"
                     /translation="LVVAGPVGYLPNILLGGLLWGDALVGADLSFRALPLAAAWASLF
                     VFPIIHALTELPTYFGYVMPRLQALTGRRALPMLLCAFVLSTQHVFLPLLFDWRFLAW
                     RFLMFLPFALWLGWVVDRRPTVLPYLAIAHGLIDLSLPILVLAASVG"
     gene            604..1650
                     /locus_tag="VLS86_09095"
     CDS             604..1650
                     /locus_tag="VLS86_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HSO50693.1"
                     /translation="MKAIVYHGPGNKAWEEVAKPTIKEDTDAIVRVDATTICGTDLHI
                     LKGDVPAVTDGRILGHEAVGTVETVGAGVKSVKPGDRVLVSCITSCGSCRYCREASYG
                     QCIGGGGWILGHKIDGTQAEFVRVPFADTSTYKVPSTVSSEDVLMLADILPTGYEVGV
                     LNGRVKPGDVVAVVGAGPIGLAAILGARLYSPAHIVAIDLADSRLDAAKRFGADVVIN
                     NSTEDPMVRILELTDGLGADVAIEAVGVPETFELAARLIRPGGYVANVGVHGKPVTLH
                     LEDLWIRNVTITTGLVDTYSTPTLLRLIENHQINAGLFATHHFDLDDFMKAYDVFARA
                     ADTGALKVVVTRKE"
     gene            complement(1731..>2113)
                     /locus_tag="VLS86_09100"
     CDS             complement(1731..>2113)
                     /locus_tag="VLS86_09100"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HSO50694.1"
                     /translation="HRIKSISGARNRMFDIKETGLFPVTEMARALALNAGVAVPSTLE
                     RLGRVAENPDWSEASTSLSQVFKALQQIRFQHQARQLDAGVRPDDLVVPGQLDRLSRL
                     QMTDAFRVLGSTLDQCAYRLDLGT"
BASE COUNT          364 a          697 c          629 g          423 t
ORIGIN      
        1 ctggtggttg ccggccccgt gggctaccta cccaacatcc tcctcggggg gctgctgtgg
       61 ggcgacgccc tggtaggagc agatctcagt ttccgtgcgc ttccgctcgc agccgcgtgg
      121 gcgagtctgt tcgtctttcc catcatccat gccctgaccg aactgccgac ctacttcggc
      181 tacgtcatgc cccgtctgca ggccctgacc gggcgacggg ccctgccgat gcttctctgc
      241 gcgttcgtgc tttcgaccca gcatgtgttc ctgccgttgt tgttcgactg gcgctttctg
      301 gcatggcggt ttctcatgtt cctgccgttc gctctctggc tcggctgggt tgtcgaccga
      361 cgacccacgg tcctgcctta cctggcaatc gcccacggtt tgatcgacct ctccctcccg
      421 atcctcgttc tcgcggcgtc ggtcggttga aacccccgtt ttggcgcgac gaactggggc
      481 tatggcacgg ttcttcgcgt cagcacgagg gaactgcctg cgtgtcaggg ccattcggcc
      541 ctgaccgggg ccagtcggtt tccccatact gatcagacac cacccctacc agaggagagc
      601 ctgatgaagg cgatcgttta ccacggcccc gggaacaagg cgtgggaaga ggtggccaag
      661 cccaccatca aagaggacac ggacgcgatt gtgcgggtgg atgccaccac gatctgcgga
      721 accgacctcc acattctcaa aggcgacgtg cccgccgtca ccgatggccg catcctcgga
      781 cacgaagcgg ttggcaccgt tgagacagtc ggcgccggcg tcaagtccgt taaaccgggt
      841 gacagagtcc tggtctcgtg catcacttcc tgcggttcgt gccgctactg ccgcgaggcc
      901 agctacggac agtgcatagg tgggggcggc tggatcctgg gccacaagat cgatggcacc
      961 caggctgaat tcgttcgggt tccattcgcc gacacctcca cttacaaggt cccgtcgacc
     1021 gtaagcagcg aagacgtctt gatgctggcc gacatcctgc cgaccggata tgaggtcgga
     1081 gttctcaacg ggcgggtcaa gcccggtgac gtcgtggccg tggtcggggc cggcccgatc
     1141 ggtctcgccg caattctcgg ggcccgcctc tacagccccg ctcacatcgt ggctatcgac
     1201 ctggccgatt ctcgcctcga tgctgccaag cgcttcggtg ccgacgttgt catcaacaac
     1261 tcgaccgaag acccgatggt tcgcatcctg gagttgaccg atggccttgg cgccgatgta
     1321 gcgatcgaag cggtcggagt gcccgaaacc ttcgagttgg cagctcgcct catccgtccc
     1381 ggtggctatg tggccaacgt gggcgtccac ggcaagccag tgaccctaca cctcgaagac
     1441 ctatggatcc gcaacgtcac gatcacgacg ggtctggtcg acacctattc gaccccaacc
     1501 ctgcttcggc tcattgagaa ccatcagatc aacgccggcc tgttcgcaac ccaccatttc
     1561 gacctggacg acttcatgaa ggcgtacgac gtcttcgccc gggcggccga caccggcgcc
     1621 ctgaaggtgg tcgtcacccg aaaggaataa cacccccacg ccacccacgg acccgtgctc
     1681 tctctgcgca gtcgcatgcg gctgcgcaga gccaattctg ggcggtccgc tcaggtaccc
     1741 aggtcgaggc gataggcaca ttgatcgagg gtcgaaccaa gaaccctgaa ggcatccgtc
     1801 atctgaagcc ggctcagccg gtcaagttgg ccgggcacga cgaggtcgtc aggccggacc
     1861 cctgcatcga gctggcgagc ctggtgttgg aatctgattt gctggagtgc tttgaagact
     1921 tgcgacaacg atgtgctcgc ctccgaccag tccgggttct cggcgacccg cccaagcctc
     1981 tcgagagtcg agggcactgc gacacctgcg ttgagcgcca gagcccgagc catctccgtg
     2041 actggaaata gtccagtttc cttgatatcg aacatcctgt ttctggcgcc ggaaatgctc
     2101 ttgatccggt gga
//
LOCUS       DATITW010000653         3398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_352073, whole genome shotgun sequence.
ACCESSION   DATITW010000653 DATITW010000000
VERSION     DATITW010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352073"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1046
                     /locus_tag="VLS86_09105"
                     /pseudo
     CDS             261..1046
                     /locus_tag="VLS86_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            complement(1543..>3398)
                     /locus_tag="VLS86_09110"
     CDS             complement(1543..>3398)
                     /locus_tag="VLS86_09110"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HSO50695.1"
                     /translation="LTVLAKWLIRTGEAPTRRLLGLTTLLASVSTLALLVALLSGDFS
                     LDYVVRTTSIATPWPYRLAALWGAMEGSLLFYATLTLFAGWIGCRRIGADLATTAAQV
                     VAGVGLGLLLLTATMASPFTTLDLPAVDGEGLLAILQHPAMVYHPPILYLGLTTLIVP
                     FSVTVAAVLARRADRGWIRITRCWLLVSWTLLTIGMVAGANWAYVELGWGGFWAWDPV
                     ENTSLMPWLATTVFLHTSRNQERAGRLVRWNVGFALLPFILTLLGVYLTRSGATGSIH
                     AFAESPVIGRILIVTAAIGLLIGAWVTLKVERGEPWERVSPLGRDTWLAVNGGLLTVA
                     LVFITVGSAYPAYLEVFVGERALVRPDFFIAVLAPIGLLLAFGLGFAFKTEWVPVGLP
                     KSRIAIYLLLALLSSMIVGTVFTSRPIIAVVLFGLAGASLLLLGLDLFRERPSGRILA
                     GHIAHIGMALVLVGGAGSSLGEDFTAAMGPGETAQLDRYTIELEDIGTGEADRFIFVV
                     ADIFFTGPGPGGFRLSPEIRAYEDQRLPVPEPALRSTPVEDVVVAISRVAGDASVVQV
                     NVFVRPLVLWVWVGAALIAFAGLIALVATGAASSGRRRSATAARQEAGTTT"
BASE COUNT          634 a         1102 c         1161 g          501 t
ORIGIN      
        1 aacagctctc cgcccaccgg gcggtggtca tggtcgacgt gagtgccgtc gaccttggcg
       61 agagcgacat ccaggtcctc gacagctacg tcagggacct gggacgtggg ctcgtcgtca
      121 tcggtggcga ggactcctac gccctgggtg cctaccagga caccgcgctc gaggcattgc
      181 tcccggtgga ctccgaggca caggacgcca agcgggaggc gaccgtggca gaagtcctcc
      241 tcatcgacac cagcgagtcg atgggggcct gtcattgcgc cgacgacggt cagttcatgg
      301 agagcaccga aggaggggtc aacaagaccg acatctccaa ggccgcagcc ctacgggcgg
      361 tggaggtgct ctccgagagc gacgaagtcg ggatcctcgc cttcagcgga gggcagaaat
      421 gggtggtccc gctccagccc ctcccctcgt cggacgtgat cgacgatggg gtgggaagtc
      481 tccggccgtt tggcgagacc cggatcgtcc ccgcgctcga cgaggcggcc gccgccctga
      541 gagaatcgga ccgggagttg aagcacatca tcctgttcac cgatggcttc accccggagc
      601 tcgacatcga gggcttcgcc aactacggcc tcgaggtggg tgacctcgtc gagtcggctc
      661 agttgctggc cgaagagggg atcaccgtat ccgtggtcgg aaccggggag ggcgcgatcc
      721 atgccctgga agaggtggcg gccgcaggca acggccgctt ctacccgggg cgggacctca
      781 atgagatccc cgagatcttc gtcaaggagg ctcgtctggc ctcgcggtcg ttcatcaacg
      841 agggtgagtt ctacccgtcg gtcacttcga cctcggcagc ggttcgagac ctggcctcct
      901 cccctgctct cctcggctat ctcgccacca cggccaagcc tatcgccgac gtccagctcc
      961 agatcggtga gttcgccgac cccttgctgg cgtcgtggcg cgtcggtctg ggccaggtca
     1021 cggcctggac cagcgatgcg ggtgacaaat gggccgccca gtgggcggga tgggacgggt
     1081 tctcggactt ctggtccaat gtggtcaggg agaccttccc actgaccggg tcggccggcc
     1141 agcgcatcga tgccagcatc gccgacgagt tgatgaccat caccctcgaa agcgccgacg
     1201 catggccggc cggcacttcc cccatggctc gggtggggta tcccgatggc acgtccgaag
     1261 aggtgagact cgagcgaaca tccgacttcg agttcgaagc ggtggtcccg gctcgtcagg
     1321 gcggtgccta tgcggtgggg gtcggtgtgg acaacgccca gggtgaggcg gtgatcatgt
     1381 cggcgatagc ctcgcgatcg ttcgccgccg agtacctgcc gggagcgtct gaccccgatc
     1441 taatggccag cgtctccctc accaccggtg gtcgtggcga gatcgcggct gcagacgcat
     1501 tcgcccccga gggcctggcc gaaggagtca acgaggtcag cctcaggtgg ttgttcctgc
     1561 ttcttgccgc gctgctgtgg ccgatcgacg tcgccctgag gaggctgcgc ctgtcgcgac
     1621 gagagcgatc agacccgcaa acgcgatcag agccgccccc acccacaccc agagcaccag
     1681 aggccgcaca aacacattga cctgcacgac agaggcatct ccggcgaccc gggagatggc
     1741 cacaaccacg tcctccacag gggtggagcg gagcgccggt tcggggaccg gcagcctctg
     1801 gtcctcatag gccctgatct cggggctcag gcgaaatccg cccggtccgg gcccggtgaa
     1861 gaagatgtcg gccaccacga agatgaaacg gtcggcctcg ccggtgccga tgtcctccaa
     1921 ctcgatggtg taccgatcca gttgcgcggt ctcccccggg cccatggccg cggtgaagtc
     1981 ctccccgagc gagctgcccg cccctccgac caggaccagg gccattccaa tgtgagcgat
     2041 gtgaccggcg aggatccgac ccgatggtct ctcccggaac aggtcgaggc ccaggagcag
     2101 caatgaggcg ccggcgagcc cgaacaggac gacggcgatg atcggcctgc tggtgaacac
     2161 ggtgccgacg atcatcgagg acagcagggc gagcaagagg tagatggcga ttcggctctt
     2221 agggagcccg accggcaccc attctgtctt gaaggcgaac ccgaggccaa aggcgagaag
     2281 caacccgatc ggggccagga ccgcgatgaa gaagtcgggc cggaccagag ctcgttcccc
     2341 gacgaagacc tcgaggtaag ccgggtaggc ggatcccacg gtgatgaaga ccagggcgac
     2401 cgtgagcagc cctccgttga ccgccagcca tgtgtcccgt cccaagggcg atacccgctc
     2461 ccacggctcg cctcgctcca ccttgagcgt gacccaggcg ccgatcagca gcccgatggc
     2521 cgcggtgacg atgaggatgc ggccgatcac aggcgattcg gcgaaggcat ggatcgaacc
     2581 ggtggccccg gaccgggtca gatagacccc gagcagggtg aggatgaagg ggagcagcgc
     2641 aaagccgacg ttccaccgaa ccagacgtcc agcccgctcc tggttgcgcg aggtgtggag
     2701 aaagacggtt gtcgccaacc agggcatcaa cgaggtgttc tccacaggat cccatgccca
     2761 aaacccgccc cagcccagct cgacatacgc ccaattggcg ccggcgacca tcccgatcgt
     2821 gagcaaggtc caggagacga gcagccagca acgggtgatg cggatccagc cccgatctgc
     2881 cctccgagcc aggaccgccg ccaccgtgac cgagaacggg acgatcaggg tggtgaggcc
     2941 cagatagagg atcggcgggt ggtagaccat cgccgggtgc tgcagtatgg cgaggaggcc
     3001 ctctccgtcc accgccggca gatccagcgt ggtgaagggc gaggccatgg tcgcggtgag
     3061 caacaggagc cccaagccca ctcctgccac cacctgtgcg gccgtggtgg cgaggtcggc
     3121 gccgattcgg cggcatccga tccacccggc gaagagggtc agcgtggcgt agaagagaag
     3181 ggacccctcc atcgcccccc aaagcgctgc cagccgatag ggccacgggg tggcgatgct
     3241 ggtggtgcgg acgacgtagt ccagggagaa atcaccgctg agcagggcga ccaggagcgc
     3301 gagggtggag actgacgcca gcagcgtggt gagcccgaga agcctgcggg tcggggcctc
     3361 gccggttcga atcagccatt tggcgagcac ggtcagcg
//
LOCUS       DATITW010000654         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_352164, whole genome shotgun sequence.
ACCESSION   DATITW010000654 DATITW010000000
VERSION     DATITW010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352164"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VLS86_09115"
     CDS             complement(<1..500)
                     /locus_tag="VLS86_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50696.1"
                     /translation="MAPVVDVLHLNKRYGDFTAVRDISFSVEDGEIFGILGPNGSGKT
                     TTVESLQGLRKIDGGSVRVLDLDPVTQASEIRRRIGSQLQESALPDRVKVWEALDLFG
                     SISPDGPSVTQLLQDWGLSEKRNDKFGSLSGGQQQRLFVALALVNDPTLVFLDEMTTG
                     LDPASRR"
     gene            658..846
                     /gene="rpmB"
                     /locus_tag="VLS86_09120"
     CDS             658..846
                     /gene="rpmB"
                     /locus_tag="VLS86_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004821237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HSO50697.1"
                     /translation="MSYRCEICGKEPSYGKTVSFSHKRNNRRWLPNIQRVRVRHGSNT
                     RRARVCTSCIRAGKVEKA"
     gene            849..1064
                     /locus_tag="VLS86_09125"
     CDS             849..1064
                     /locus_tag="VLS86_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HSO50698.1"
                     /translation="MLQGVVKTYDPVTGAGIIVREDDRSVVFLRPGSLRGSIFRTLRQ
                     GQRLVFETVEEDGNVFASSVAMGQEGY"
     gene            1165..1398
                     /locus_tag="VLS86_09130"
     CDS             1165..1398
                     /locus_tag="VLS86_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSO50699.1"
                     /translation="MVQAYVLIQTEVGKAALVAEEVREIPGVDSADDVTGPYDVIVKA
                     TSEDVDSLGKLVVAKIQAIDGITRTLTCPVVHL"
     gene            complement(1450..>2013)
                     /locus_tag="VLS86_09135"
     CDS             complement(1450..>2013)
                     /locus_tag="VLS86_09135"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50700.1"
                     /translation="IGIEIADDWATVLALKRSSSFFDQGAVVEPFLTGSRDLQVAVRT
                     YPKWEMSAVEAPARATGGIYTYDQKYLAWGGEVSGGRELPATLTPELADRIRGLSLRV
                     TEVAGLRGIARIDYLERDGEISVNEVNTIPGSMGAYLWIDPPISREQLFRDMIEEART
                     GPVRVFSVAGSDGTALRSAGTIASKLG"
BASE COUNT          359 a          668 c          619 g          367 t
ORIGIN      
        1 cgacgggagg ccggatcgag accggtggtc atctcgtcga ggaagaccag cgtgggatcg
       61 ttgaccagag ccaacgcaac gaacagccgc tgctgctggc caccggacag actcccgaac
      121 ttgtcgttcc tcttctcaga cagaccccag tcttggagaa gctgggtcac cgaagggccg
      181 tcggggctga tggagccgaa gagatcgagt gcttcccaca ctttcacccg gtcggggagg
      241 gcggactcct gcagttggga gccgattctt cgccggatct ccgacgcctg ggtgacgggg
      301 tcgaggtcga ggaccctcac cgatccgcca tcgatcttcc tcaggccttg gagactctcc
      361 acggtggtcg tctttcccga gccgttgggc ccgagaatgc caaatatctc gccgtcctcc
      421 accgagaacg agatgtcgcg caccgcggtg aagtccccat accgcttgtt cagatggagg
      481 acatcgacca ccggagccat gtgatgattc tcctgcccgg gaaagccggg atatagggtc
      541 aaaggtccgt acccggatgg cccgggctgg gagttggcct gagccccggc tagtcttccc
      601 cccggtcgga ccgtccacgg ccgtctattc ttccagcccg tcccaggagt tttccagatg
      661 tcctatcgct gcgagatctg cggcaaagag ccgagctacg gcaagaccgt cagcttctca
      721 cacaagcgca acaaccgccg ctggcttccc aacattcagc gggtccgggt ccgccacggc
      781 tcgaacaccc gacgcgcccg ggtctgcact tcctgcatcc gggccggcaa ggtggaaaag
      841 gcctgagcat gttgcagggc gtggtcaaga cctacgaccc cgtgaccgga gccgggatca
      901 tcgtccgcga ggacgaccgc tcggtagtgt tccttcgtcc cgggtctctt cgaggatcga
      961 tcttccgcac cctgcgccag ggccagcgtc tggtcttcga gaccgtcgag gaagacggca
     1021 acgtgttcgc ctcgtcggtg gcaatgggcc aagagggcta ctgagggcgg agccttcgcc
     1081 ctgcaggggt cggggcgata ccgccaccac gaccctcgat gcaatcaact agggtcgaaa
     1141 ctccccagac cataggaggc tcagttggtc caggcctatg tcctgatcca gaccgaggtc
     1201 ggcaaggccg cccttgtcgc agaggaagtt cgcgagatcc ctggcgtcga ttcggccgac
     1261 gacgtgaccg gtccctatga cgtgatcgtc aaggccacca gcgaagatgt cgactcgctc
     1321 ggcaagctgg tggtggccaa gatccaggcc atagacggca tcacccgcac ccttacctgc
     1381 ccggtggtgc atctctgatc cgggctcgaa tcgactgaga ccgggccggc attcgccggt
     1441 ctttattggt tagcccagtt tggaggcgat tgtcccggcg ctgcgcagcg cggtgccgtc
     1501 gctgcccgcg accgagaaga cgcgcaccgg cccggtcctt gcctcctcga tcatgtcacg
     1561 aaacaactgc tcccggctga tcggtggatc gatccaaagg taagccccca tcgatccggg
     1621 gatggtgttc acctcgttca ccgagatctc cccgtcccgt tccaagtagt cgattctggc
     1681 tatgcccctg agcccggcca cttcggtcac ccggagggac aatcccctga tccgatctgc
     1741 gagctccgga gtgagggtcg ccggcagctc cctgcccccg ctcacctctc cgccccaagc
     1801 caggtatttc tggtcatacg tgtagatgcc tccggtggcg cgggcagggg cctccaccgc
     1861 cgacatctcc cacttggggt aggtgcgcac cgccacctgg aggtcgcggc taccggtgag
     1921 gaacggctcg acaaccgcgc cctggtcgaa gaacgaagag ctccgtttca gggcgaggac
     1981 cgtggcccag tcgtctgcga tctcgattcc gat
//
LOCUS       DATITW010000655         1888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_232605, whole genome shotgun sequence.
ACCESSION   DATITW010000655 DATITW010000000
VERSION     DATITW010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232605"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..448)
                     /locus_tag="VLS86_09140"
     CDS             complement(41..448)
                     /locus_tag="VLS86_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HSO50701.1"
                     /translation="MSVVLLDTTFLIDAERADIDLDDLIHEDDDIAIAAITVAELVVG
                     VELASRHAKANRQAYVDDILASVPVISYDRDIAFEHAQLLVAVRQTGRPRGAHDLLIA
                     ATARATKRMVLTADESAFADLPGVTVRHYRINP"
     gene            complement(809..>1888)
                     /locus_tag="VLS86_09145"
     CDS             complement(809..>1888)
                     /locus_tag="VLS86_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HSO50702.1"
                     /translation="FTPERSPAEGTVFDHCRRALDRALSRLGRRGLPLIGTGDWNDGF
                     NRVGREGRGESVWLGWFLEANLSRFADIADELREAELAATWRRGALRLRASLEAEAWD
                     GDWYRRAFFDDGTPLGSSDNPECRIDSIAQSWSVIHGGAPGDRARRAMDSVEEYLVHR
                     GDGLVLLFTPPFDEWDVDPGYIKGYLPGVRENGGQYTHAAIWCVIAFATLGDGDRAGE
                     LFGLLNPINHASTRAGIHRYRVEPYVAAADVYSKAPHVGRGGWTWYTGSAGWMYRAGV
                     EWILGFRLRGTGLLIDPCIPRAWRGFEIDFRYHSARYEIQVSNPNGVTRGVISLTLDG
                     TTLAPGSELKLVDDGLTHQVRVVLG"
BASE COUNT          333 a          609 c          572 g          374 t
ORIGIN      
        1 ttccgttccg tttccctatg gcctgagctg gagtggaggt ctacgggttg attcggtagt
       61 ggcggacggt cacaccaggt agatctgcga aggctgattc gtcggcggtg agcaccatgc
      121 gttttgtggc gcgagctgtg gccgcgatga gcaagtcgtg ggcgccgcga ggacgccctg
      181 tctgccttac cgccaccaga agctgagcat gttcgaaagc gatatcccgg tcataggaaa
      241 tgactggaac cgaggcaagg atgtcgtcca cgtaagcctg tcggttggcc ttggcatgcc
      301 gggatgcgag ttcgacccct accaccaatt cggcgaccgt gattgctgca attgctatgt
      361 cgtcgtcttc gtggatcaga tcgtcgagat cgatgtcggc tcgctcggcg tcgatcagga
      421 aagtcgtgtc gagaagcacg acgctcatga tctgttgtcg atctccacca actcgcggag
      481 acgtgagagc tcctctgccc aagctgaatc tggtctgtga cgcttcagga tctccctcac
      541 ctcggagcca cggcccctgt tgatcggctc gagatgtgcc acggcctttc cacgccgaac
      601 gatcgtgtag tgctcgttcg cgtgctcaac gctgtccagc aaatcggcga agcgtcttgc
      661 tgcttcggtg gctgagatat ctggcatgag atcagattat cagatttcta cgatccaggg
      721 gagtctgggc agctgagcga ctacccgtcc cgtttcccta gctttcagga ttgctcgacg
      781 ttgcggctcg ctccgtcgag tgtgacgctc agcccaagac aaccctgacc tggtgggtca
      841 atccgtcatc gaccagtttc aactccgagc cgggggccag ggtcgtgccg tccagtgtca
      901 gactgatcac ccccctggtg accccattgg gattgctgac ctggatctca tagcgggcgg
      961 agtggtagcg aaagtcgatc tcgaagcctc tccaggcgcg cggtatgcat ggatcgatca
     1021 gaagcccggt tcctcgtagg cggaagccga ggatccactc cacaccggcc cggtacatcc
     1081 accccgcaga acccgtgtac caggtccaac ctccccgccc cacgtggggc gcctttgaat
     1141 agacgtccgc tgctgccaca tagggctcca cccggtaccg gtggatcccc gctctggtgc
     1201 tggcatggtt gatcgggttg agcagaccga acaactcgcc cgcccgatca ccgtcgccga
     1261 gggtggcgaa ggcaatgacg caccaaatgg cggcatgggt gtactggccg ccgttctcgc
     1321 gtacgccggg caagtacccc ttgatgtatc ccggatcgac gtcccactcg tcgaacggtg
     1381 gggtgaagag cagcaccaga ccgtcccctc gatggaccag gtattcctcc accgagtcca
     1441 tcgcccgacg ggctcggtcg cccggtgctc cgccgtggat gaccgaccac gattgcgcga
     1501 tcgagtcgat tcgacattcc gggttgtccg atgaaccgag cggtgtccca tcgtcgaaga
     1561 aggcgcgccg gtaccagtcg ccgtcccacg cctcggcctc gaggctcgcc cggagacgaa
     1621 gcgctccccg ccgccaggtg gcggcgagct ccgcctccct caactcgtcg gcgatgtcgg
     1681 cgaatcggct caggttggcc tcgaggaacc atccgagcca cacgctctct cctcgcccct
     1741 cgcgccccac ccgattgaag ccgtcgttcc aatcaccggt gccgatcagg ggaaggcctc
     1801 gtcttcccag cctggacagc gccctgtcga gagcacggcg acagtggtcg aacaccgttc
     1861 cttccgcggg cgatcgctcc ggggtgaa
//
LOCUS       DATITW010000656         1714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_112782, whole genome shotgun sequence.
ACCESSION   DATITW010000656 DATITW010000000
VERSION     DATITW010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112782"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VLS86_09150"
     CDS             complement(<1..403)
                     /locus_tag="VLS86_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50703.1"
                     /translation="MDDRVLVAVDVPLVGDERHKNWAKVVESVDSAKASGWAFEGPFV
                     ATGGIQDVPIGSVLLVYGERGSRANPQIAARVYRVNPDSTISLEAEAKGKAWARTLRD
                     PIERCLGMDSALPDLSYLSDELLADELRARGW"
     gene            470..1468
                     /locus_tag="VLS86_09155"
     CDS             470..1468
                     /locus_tag="VLS86_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSO50704.1"
                     /translation="MAKLRLFANLREIAGTSRLDVASDTVGGVIEAAAEKFGPEFRRG
                     LETSRVWINGEAAAMDDAVAEDDEVVLIPPVSGGSQRAATLSTVDLIGFIPLAVALLA
                     ILANTQGQEIWAAVLVAIVAVWAVDLESAFAARGKEFASLAVIVTAAVSAMAAHVMGG
                     DGYALAAVIAVAVGLGWAVAFSRYRQVDVFSPTLMVSLFAGLGTASLVLVRSAHTLEE
                     SAADVFLVSVIVGLFLGALVERLPALPLLDPFSVTAIGAILAAVAAALLWDLDLVAYL
                     LVGLGLAVALVSGRGFSSMLRRGRVALTERPPGVLISLDGVVLGAAIYYPLLQLIF"
     gene            complement(1486..>1714)
                     /locus_tag="VLS86_09160"
     CDS             complement(1486..>1714)
                     /locus_tag="VLS86_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HSO50705.1"
                     /translation="LAGATVIRGIEGFGKSSRLHTSHILRLSEDLPIVIECVDAAENI
                     ESVLPLLDEMIGDGLVTMEKVEVRVYRADRD"
BASE COUNT          242 a          570 c          563 g          339 t
ORIGIN      
        1 tccatcctcg ggctcgcagc tcgtccgcga gaagctcatc gctgagatag gacaagtcgg
       61 gcagtgcgga gtccattccg aggcagcgct cgatcgggtc acgcagggtg cgcgcccacg
      121 ctttaccctt ggcctcggcc tcgagactga tggtcgaatc ggggttgacc cggtagacgc
      181 gggccgcgat ctgagggttg gcccgcgacc cacgctcccc gtagacgagc agcaccgatc
      241 caatcggcac gtcctgaatc ccgcccgtgg ccacgaatgg gccctcgaag gcccaccccg
      301 aagccttggc gctgtcgacg gactcgacca ccttcgccca gttcttgtgg cgctcgtccc
      361 cgaccagggg gacatcgacc gccaccagca ccctgtcgtc catccttctg agcgtaaacg
      421 acgaaaagga gagccgcgat gctgcttcgg tacgctttac cctctgtcta tggccaagct
      481 ccgtctcttc gccaacctac gggaaatcgc cggcacgtcg aggctcgacg tcgcttcgga
      541 caccgttggc ggcgtgatcg aggccgccgc cgagaagttc ggcccggagt tccgacgcgg
      601 gctggagacg tcgcgggtct ggatcaatgg tgaggccgcg gcgatggacg atgcggtcgc
      661 cgaggacgac gaggtggttc tcatccctcc ggtgtccggt gggagccagc gggccgccac
      721 cctgtccacc gtcgacctga tcggcttcat cccgctggct gtcgccttgc tggcgatcct
      781 ggccaacacc cagggtcagg agatctgggc cgcggtgctg gtggcgatcg tcgcggtatg
      841 ggccgtcgac ctcgagtcgg cgttcgcggc gaggggcaag gagttcgcct cgctggcggt
      901 gatcgtcacc gccgccgtct cggcgatggc ggcacatgtc atgggcggcg acgggtatgc
      961 cctcgccgcc gtgatcgcag tggccgtagg tctcggatgg gccgtcgcct tttcccggta
     1021 tcgacaggtg gacgtgttca gtccgacgct catggtgtcg ctgttcgccg gactgggcac
     1081 cgcctcgctg gtcctggtcc gttcggcgca caccctcgag gagagtgctg ccgacgtctt
     1141 tctggtatcg gtcatcgtcg ggcttttcct cggggccctg gtcgagcgtc tgccggctct
     1201 ccctttgctc gacccgttct cggtgaccgc gatcggggcc atcctggccg cggtggccgc
     1261 agccttgtta tgggacctcg atctcgtggc ctacctgctc gtcggtctgg gcttggcggt
     1321 cgccctcgtc tccgggcgcg gcttctcctc gatgctgagg aggggtcggg tcgcactcac
     1381 cgaacgcccg ccgggcgtcc tgatctcact cgacggggtg gtgctgggcg ctgcgatcta
     1441 ttacccgttg cttcaactga tcttctgagg tcttgctcga gcaaatcagt cgcggtcagc
     1501 ccgatacacc cgtacttcga ccttttccat cgtgaccaga ccgtccccga tcatctcgtc
     1561 gaggaggggg agaacggatt cgatgttctc ggcggcatcg acgcactcga tgacgatggg
     1621 caggtcctcg gagagacgca ggatgtgaga cgtgtgcagt cgactcgact tcccgaaccc
     1681 ctcgatgccc cggatcaccg ttgctccggc cagg
//
LOCUS       DATITW010000657         1871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_713498, whole genome shotgun sequence.
ACCESSION   DATITW010000657 DATITW010000000
VERSION     DATITW010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713498"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VLS86_09165"
     CDS             <1..434
                     /locus_tag="VLS86_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HSO50706.1"
                     /translation="AINQLVPVCRAKVSLRTAPGQNTAAAARALKDHLIANAPWGTKV
                     DFIYEESGDASILDTDNFAVKAWTEAFTEGYGTEPVAMGAGGSIPFIATFAELFPEAP
                     ILVIGASDPTSAYHAPNESQDLGDLEKAVLSEAIAFQLLAG"
     gene            complement(453..914)
                     /locus_tag="VLS86_09170"
     CDS             complement(453..914)
                     /locus_tag="VLS86_09170"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HSO50707.1"
                     /translation="MFDIVFFRPEIPPNTGNAMRLAAVTGSRLHLIEPLGFDMDDAKL
                     RRAGMDYRDKAVVSVHPDLDDWRESAKPRRVFALTVDGDRIYTDVEFEEGDAFVFGPE
                     SVGLPQEVKTVSWMTGTLRLPMVPGVRSLNLSNSAAVVIYEAWRQHGFSGS"
     gene            complement(917..1585)
                     /gene="nucS"
                     /locus_tag="VLS86_09175"
     CDS             complement(917..1585)
                     /gene="nucS"
                     /locus_tag="VLS86_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016469832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HSO50708.1"
                     /translation="MRLVIARCTVDYSGRLTAHLPEAVRLIMVKADGTLAIHSDGNAS
                     KPLNWMVAPNRVETFEDRWEVANNKGEKLTIWIAEIIEDVTHSLGVDPGLQKDGVEAH
                     LQELIAARPWVLEKGLTLIRREYPTDIGPVDLLCTDSDGRSVAVEVKRRGEIDGVEQL
                     ARYMERLGNDPRLGQVRGLFVAQSIAPQASVLATARGIEAIEIDYDGLRDIEDTEFRL
                     FDIR"
     gene            complement(1582..>1871)
                     /locus_tag="VLS86_09180"
     CDS             complement(1582..>1871)
                     /locus_tag="VLS86_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50709.1"
                     /translation="VLVPAALLLGSGAIAWNAVGMLAVMDFSPSDLVGRGTGLVMLGF
                     LTGVGLGAPLMGLSVDRLGVYTPGWVVVATLFASAALVAVKIHRTGTLAHV"
BASE COUNT          347 a          654 c          564 g          306 t
ORIGIN      
        1 aggcgatcaa ccaactggtc ccggtgtgcc gcgccaaggt cagtcttcga accgcacccg
       61 gccagaacac cgcggctgcg gcccgggcac tcaaggatca cctgatcgcc aacgcaccgt
      121 ggggcaccaa ggtcgacttc atctatgagg aatccggcga cgcatcgatc ttggacaccg
      181 acaacttcgc ggtgaaggca tggaccgaag ccttcaccga ggggtacggg accgagccgg
      241 tggcgatggg agccgggggc tcgatcccgt tcatcgccac cttcgccgag ctgttccctg
      301 aggccccgat cctggtcatc ggggcctccg atccgaccag cgcctatcac gcgccaaacg
      361 agagccagga cctcggcgac ctggagaagg cggttctctc cgaggcgatc gcgttccagc
      421 tgttggccgg gtgagcccgt cttggggttc gctcaggagc cgctgaaccc gtgctgtctc
      481 catgcctcgt agatgaccac ggcagccgag ttggagagat tcagactgcg gaccccgggc
      541 accatcggca gccgcaaagt gccggtcatc catgacaccg tcttgacctc ctgggggagc
      601 ccgaccgact ccggcccgaa cacgaaggcg tcaccctcct cgaactcgac atcggtgtag
      661 atgcgatcgc catcgacggt gagggcaaac acccgccgcg gtttggcgct ctcccgccaa
      721 tcgtccaaat cggggtgcac cgagaccacc gccttgtccc gataatccat cccggcgcgc
      781 cgcagcttgg cgtcgtccat gtcgaagccg agcggctcga tgagatgcag ccggctgccg
      841 gtgaccgcag cgagacgcat cgcattcccg gtgttgggcg ggatctcggg acgaaagaag
      901 acgatgtcga acacggtcag cgaatgtcga agagacggaa ctcggtgtcc tcgatgtcgc
      961 ggagcccgtc gtagtcgatc tctatcgcct cgatcccccg tgccgtcgcc agaaccgacg
     1021 cctgaggcgc tatcgactgc gccacgaaca gccccctgac ctgccccagc ctcgggtcgt
     1081 tgcccagcct ctccatatag cgcgcgagct gctcgacccc gtcgatctct ccccttctct
     1141 tcacctcgac cgccacactg cgaccatccg agtcggtgca cagaaggtcg accggcccga
     1201 tgtcggtggg gtactccctg cggatcaagg tcaaaccctt ctccagcacc catggtctgg
     1261 ccgcgatcag ctcctgcagg tgtgcctcca ccccgtcctt ctgcagcccg gggtcgacgc
     1321 cgagcgagtg ggtcacgtct tcgatgatct cggcgatcca gatggtcagc ttctcgcctt
     1381 tgttgttggc gacctcccaa cgatcttcga aggtctcgac ccggttgggg gcgaccatcc
     1441 agttcagcgg cttggacgcg ttcccgtcgg agtgaatggc gagagtgccg tcagctttga
     1501 ccatgatcag ccgcaccgcc tcagggagat gagcggttag acggcccgag tagtcgaccg
     1561 tgcagcgagc gatgaccagc ctcaaacgtg ggcgagagta cccgtgcgat ggatcttcac
     1621 cgccaccagc gccgctgagg cgaacagggt cgccaccacc acccatcccg gggtgtacac
     1681 cccgagacga tcgactgaca gccccatcaa gggggcgccg agcccgaccc cggtcaagaa
     1741 ccccagcatc accaacccgg ttcccctgcc caccaggtct gagggagaga agtccatcac
     1801 ggccagcatc cctacggcgt tccaggcgat agcgcccgag ccgaggagca gcgctgcggg
     1861 gaccaacacc c
//
LOCUS       DATITW010000658         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_753322, whole genome shotgun sequence.
ACCESSION   DATITW010000658 DATITW010000000
VERSION     DATITW010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753322"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VLS86_09185"
     CDS             <1..463
                     /locus_tag="VLS86_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSO50710.1"
                     /translation="IADAMEPLGIGVATGEHCHNRVMFKQFFQSDALSFAQVDPCRLG
                     GVNEVLAVLLMAAKFDVAVCPHAGGVGLCEYVQHLAIFDYLAVSGSSEGRVVEYVDHL
                     HEHFVDPAIVRGGRYVVPTAPGTSITMHPASLSAYSYPLGAEWSGILVDGV"
     gene            463..1485
                     /locus_tag="VLS86_09190"
     CDS             463..1485
                     /locus_tag="VLS86_09190"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSO50711.1"
                     /translation="MGEGAVLVTGALGCIGAWTVRDLVMSDVPVVAFDRSTDPRRLRQ
                     ITTQEQFEQVRLIHGDLADLADLQETLVANHVDRVVHLGALQIPFCRADPPLGALVNV
                     VGTVNVFEAARWVGIPHIVYSSSIAVFDWGPERGLLTVDAPARPVSHYGAYKLANEGT
                     ARAYWADFGVSSIGIRPMTVYGPGRDQGLTSSPTRGILAAVLGHRYEIGFGGTTLFHY
                     AGDVGRALAVAAHTPGAGAHVVNLNGVRASISDVVASLQDLVGSSAEGITFSPSALPF
                     PDDVDTSGLDMIGPPSVTPLNEGIAATVEFFRDLQSRGALEVGEHGLVVGDGVVLDRE
                     SVPSAE"
     gene            1491..2342
                     /locus_tag="VLS86_09195"
     CDS             1491..2342
                     /locus_tag="VLS86_09195"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSO50712.1"
                     /translation="MAAGVIDAHHHFWDPRTVDYPWMTGAYEPLRKKYGPSDLAPLLD
                     AVGVTATIVVQARQELDETHSLLEMADATPWVAGVVGWVDVTKADVAETIARIREGEH
                     GNRLVGIRHLVHDEPDPEWLLRDDVLRGLRALAEAGLVYDLLVRTRELPAATEVARRL
                     PGLRFALDHLAKPPISTRQIEPWASAIGEIARLPNVTCKVSGLVTEADWSSWRSDDLT
                     PYIAKALELFGSDRLMWGSDWPVCILAATYSEVFETAVEILTDLVGDELEAILGGCAA
                     STYGFEA"
     gene            complement(2396..>2520)
                     /locus_tag="VLS86_09200"
     CDS             complement(2396..>2520)
                     /locus_tag="VLS86_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HSO50713.1"
                     /translation="AVEIGAAHGALTMTTPGDTSMATSSEVMRAVDAQSARVVR"
BASE COUNT          425 a          792 c          817 g          486 t
ORIGIN      
        1 aatcgccgat gcgatggagc cgcttggcat cggcgtggcc accggcgagc attgtcataa
       61 ccgggtgatg ttcaaacagt tctttcagag tgacgcactc agctttgccc aggtcgaccc
      121 gtgccggctg ggaggggtca acgaggtgct ggccgtgctt ctcatggcgg ccaagttcga
      181 cgtcgcggtg tgccctcacg ccggtggcgt cgggctctgc gaatatgtgc agcatctggc
      241 gatcttcgac tatctggcgg tgagcggctc atcggaaggg cgcgtcgtcg agtatgtcga
      301 ccatctccac gagcacttcg tcgacccggc gatcgtgaga ggcggtcgct acgtggtacc
      361 aaccgccccg gggacgagta tcaccatgca tcccgccagc ctcagcgcct actcctaccc
      421 gctcggggct gaatggtcgg gaatcctggt cgacggcgtt tgatgggaga aggcgcagtc
      481 ctggtcacgg gtgccttggg ctgtatcggg gcatggactg ttcgtgattt ggtgatgagc
      541 gacgttccgg ttgtggcatt cgatcggtcc accgacccga ggcggttgcg gcagatcacg
      601 actcaggaac agttcgagca agtgcgcctc atccacgggg acctcgctga cctggcagat
      661 ctccaggaga cacttgtcgc caaccatgtg gacagagtgg tccatctcgg agcccttcag
      721 atccccttct gtcgggccga cccgccactt ggtgcacttg taaacgtcgt ggggaccgtg
      781 aacgtcttcg aggccgcccg ttgggtggga atcccgcaca tcgtttattc gagttcaatt
      841 gcggtcttcg actggggtcc cgagcgcggt cttctcacgg tcgatgctcc ggcacgaccg
      901 gtcagccact atggggcgta caagttggcc aacgaaggca ccgcccgtgc ctactgggcg
      961 gatttcggtg tgtcgagcat cggtatcaga ccgatgacgg tgtatgggcc gggacgggac
     1021 caggggctca ccagctcacc caccagaggc atcctggccg ccgtgctcgg acacaggtat
     1081 gagatcggtt tcggtggtac cacgctgttc cactacgccg gagacgtggg tcgtgcgctg
     1141 gcggttgccg cacacacgcc gggcgccggg gcacatgtgg tgaatctgaa cggcgtccgc
     1201 gcatccatct ccgacgtcgt cgcctccttg caggatctcg tcggctcatc ggcggagggg
     1261 atcaccttca gcccgagtgc cctgcccttc ccggacgacg tggacacatc ggggctcgac
     1321 atgatcgggc cgccatccgt gacaccgctc aacgaaggca tcgccgccac cgtggagttc
     1381 ttccgagacc tgcaatcgcg aggagccctc gaagtcgggg agcacggtct cgtcgtcggc
     1441 gacggtgtcg tccttgaccg ggaatcggtc ccgagcgcag agtgaggacc gtggccgcgg
     1501 gcgtcatcga cgcccaccac cacttctggg accccaggac cgttgactat ccgtggatga
     1561 ccggtgccta cgagccactg cggaagaagt atggaccctc cgatctcgcc ccgctcctcg
     1621 acgcagttgg ggtgacggcc acgatcgttg tgcaggcccg gcaggagctc gacgagacgc
     1681 attccctgct cgagatggca gacgcgacac catgggtggc aggggtcgtt gggtgggtcg
     1741 acgtcaccaa ggccgatgtg gcggagacca ttgccaggat ccgtgaagga gaacacggga
     1801 atcggttggt cgggatccgg catctggttc acgacgagcc cgaccccgaa tggctgcttc
     1861 gcgacgatgt cctacgagga ctgcgcgcct tggccgaagc cggattggtc tacgacttgc
     1921 tggtgaggac ccgcgagctg cctgcagcca ccgaggtggc ccggcgactc cccgggcttc
     1981 gcttcgctct cgatcacctg gccaagcctc cgatctcgac gcgacagatc gagccgtggg
     2041 cctccgccat cggcgagatc gccaggctcc ctaacgtcac ttgcaaggtg tctggcctgg
     2101 tcaccgaagc cgactggtcg tcatggcgtt cggacgacct gacgccttac atcgccaaag
     2161 ctctggagct gttcggctcc gatcgactga tgtggggatc cgactggccg gtgtgcatcc
     2221 tggcagcgac gtattccgag gtgttcgaga cagcagttga gatcctcacc gacctggtgg
     2281 gagacgagct cgaggcgatt ctcggggggt gtgccgcatc gacctatggt ttcgaggcct
     2341 agacgtcccg caccactctc catcaagacg tatcgcaatc aggctcgtcg tcgcttcacc
     2401 gcaccacacg ggcgctctgg gcgtcgactg ctctcatcac ttccgagctc gtcgccatcg
     2461 aagtgtctcc aggcgtcgtc atagtcaagg ctccatgggc ggcaccgatc tccacggcct
//
LOCUS       DATITW010000659         1639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_232749, whole genome shotgun sequence.
ACCESSION   DATITW010000659 DATITW010000000
VERSION     DATITW010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232749"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="VLS86_09205"
     CDS             <1..1624
                     /locus_tag="VLS86_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HSO50714.1"
                     /translation="SRVVGRHREWEAAMNQRFGQVLIVLVTGLVLGLGVFLVTKNGDP
                     PASGNSTLPQSGATTTIPGDPGVSSLPGETTTTTPGGTQPPGERPADTVPGFTVGQPW
                     GTTVGLTMFRGNPTRSYFGTGDVSQEPSVQWSYPDSAMCSTSTNLGDTTQWCGMGWTG
                     QPAVWERPDGITEIIFGAYDSAVHFVDAATGEDLRAEFQTGDLIKGSVTVDPDGYPLL
                     YFGSRDNKYRILGLDEGDPVELWSMDATDVSGTWNDDWDSNGAVVDDILYVGGENSWF
                     FAVELNRGYDADGKVTIDPQILVEMPGYNSDLIDTAGGNVSIENSPVVYDQRVYFANS
                     AGRIVGLDVSQVREGEAPIVFDYWAGGDIDASLVVDAEGMLYAAVNVKPDQVGPGYRT
                     QANIDRTREVGQLLKLDPYTDGDARIWGVDLTAGSEQSGTWSTPAVYQGVVYNNTMQG
                     SLIAVDAVNGEVLWSDEVGWHSWSSPAVVEGMLVTATCLGDVRGYSLADPRAPVQSWS
                     VSLGETCLEATPAVWDGVIYIGSRDGYLRALS"
BASE COUNT          289 a          529 c          539 g          282 t
ORIGIN      
        1 gagccgggtt gtgggccgcc atcgtgaatg ggaggccgcg atgaaccagc gtttcggcca
       61 ggtcttgata gtcctggtga ccggtctcgt cctgggtctg ggtgtcttct tggttaccaa
      121 gaacggtgac ccacctgctt ccgggaacag cactctcccc cagtccggag cgaccaccac
      181 catccccggg gacccgggtg tctccagcct gcccggggaa accaccacca cgaccccggg
      241 aggcactcaa cctccaggtg agcgtcccgc cgacacggtt cccgggttca ccgtgggaca
      301 accctggggc accacggtcg ggctgaccat gttccgtggg aacccgaccc ggagctactt
      361 cggcaccggc gacgtcagcc aggagccgtc ggtccaatgg tcctatccgg acagtgcgat
      421 gtgcagcaca tcgaccaacc tcggcgacac cactcagtgg tgtggcatgg gctggaccgg
      481 ccagccggcg gtgtgggaac ggcccgacgg gatcaccgag atcatcttcg gcgcttacga
      541 ctccgcggtt cacttcgtcg atgccgcgac cggagaggat ctgagagccg agttccagac
      601 aggggacctg atcaagggct cggtgaccgt cgaccccgac ggttaccccc tcctctattt
      661 cggatcccgg gacaacaagt accgcatcct cggtctggac gaaggcgacc cggtcgagct
      721 ctggtcgatg gatgcgacag acgtatcggg cacctggaac gacgactggg actccaacgg
      781 ggcggtagtc gacgacattc tctatgtcgg gggcgagaac agctggttct tcgccgtgga
      841 gctgaaccgg ggctacgacg ccgacggcaa ggtgaccatc gatcctcaga tcctggttga
      901 gatgcccggg tacaacagcg acttgatcga taccgccggg ggcaatgtct cgatcgagaa
      961 ctctccggtg gtctacgacc agagggtcta ttttgccaac tcggcgggcc ggatagtcgg
     1021 cctcgatgtg agccaggtcc gggaaggcga ggcgcccatc gtcttcgact actgggccgg
     1081 cggggacatc gacgcctcgc tggttgtcga tgccgaggga atgctctacg ccgcggtgaa
     1141 tgtcaagccc gaccaggtcg ggcccgggta tcggacccag gccaacatcg accgcacccg
     1201 tgaggtgggt cagctgctca agctcgaccc ctacaccgat ggcgacgcac gaatctgggg
     1261 tgtcgacctc accgccggca gcgagcagtc aggcacctgg agcactcccg cggtgtacca
     1321 gggcgtggtc tacaacaaca ccatgcaggg gagcctcatc gcggtcgatg ccgtcaacgg
     1381 cgaggttctc tggtcggacg aggtcgggtg gcactcgtgg tcctcacccg cggtggtcga
     1441 agggatgctg gtcaccgcca cctgtctagg agacgtccgt ggctactcat tggccgatcc
     1501 ccgggccccg gtccagtcgt ggtcggtcag cctcggcgag acctgtctcg aggccacacc
     1561 ggcggtctgg gacggggtca tctacatcgg ctcccgagac gggtatctgc gggccctcag
     1621 ctgatcgcgt cggtcacgc
//
LOCUS       DATITW010000660         2304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_553469, whole genome shotgun sequence.
ACCESSION   DATITW010000660 DATITW010000000
VERSION     DATITW010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553469"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VLS86_09210"
     CDS             <1..515
                     /locus_tag="VLS86_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSO50715.1"
                     /translation="IGLETLFTFTRTYVEDRQIGSTGIFFGVYGVAAAAMRILGGRHY
                     DRIPQRPLLAWALVSYGLSLGVLAVAASVPLLVLAAILGGVAHGAVFPVLTSQVVSRA
                     RTAERGSAMSIFTSIFDIALLLAAPTVGFLIDGFDYRLAFGAVAIALGAGAVVFAIWD
                     RRTVVDPAVA"
     gene            complement(517..987)
                     /locus_tag="VLS86_09215"
     CDS             complement(517..987)
                     /locus_tag="VLS86_09215"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HSO50716.1"
                     /translation="MTHPTSPDSYLEPMRQALRAAAEAESSGDVPIGAVLLDDSGRLV
                     TVDRNRREELSDPTAHAEILVMSARARELGDWRLTGHTLVVTLEPCAMCAGAAVLARL
                     DRIVYGAADLKAGAAWSLYNIPQDRRLNHRVELIDGVLAEESALLLERFFADRR"
     gene            1036..1126
                     /locus_tag="VLS86_09220"
     tRNA            1036..1126
                     /locus_tag="VLS86_09220"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1072..1074,aa:Ser,seq:gga)
     gene            1189..1279
                     /locus_tag="VLS86_09225"
     tRNA            1189..1279
                     /locus_tag="VLS86_09225"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1225..1227,aa:Ser,seq:cga)
     gene            complement(1318..2019)
                     /locus_tag="VLS86_09230"
     CDS             complement(1318..2019)
                     /locus_tag="VLS86_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016910140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSO50717.1"
                     /translation="MAEARRILVVDDEPQIVRALAINLKALGYRVDTAGSGEEALRKA
                     ADHRPDTVILDLGLPGIDGIEVIRGLRGWSSVPIIVLSVRDAEREKIAALDAGADDYV
                     TKPFGMGELLARLRAALRRESDSGEDQPTVVTDDFSIDLAKKVVTGASGEEIRLTPTE
                     WGILEMLVRHRGRLVTQTQLLKEVWGPQYGEETNYLRVFMAQLRRKLEPQPSNPRYFK
                     TEPGMGYRFEPPEAG"
     gene            complement(2012..>2304)
                     /locus_tag="VLS86_09235"
     CDS             complement(2012..>2304)
                     /locus_tag="VLS86_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSO50718.1"
                     /translation="SAGEIARRIDLRISDRGPGIPIDSRETVFQPFQRLGDSSRSEGV
                     GLGLAVSRGLLEAMGNELIIEDTPGGGTTMVIAMKIAGVIETEVEDAGLSHG"
BASE COUNT          332 a          785 c          752 g          435 t
ORIGIN      
        1 gcatcgggct cgagaccctg ttcacgttca ccaggaccta tgtggaggac cgacagatcg
       61 gctccacagg gatcttcttc ggcgtctatg gagtagccgc ggccgcgatg cgaatcctcg
      121 gcgggcgcca ctacgaccgg atcccacaga gaccgctcct ggcctgggcc ctggtctcat
      181 acggcctcag cctgggcgtc ctggcagtcg ccgcctccgt cccgttgctg gtgctcgccg
      241 cgatcctcgg tggcgtcgcc cacggtgcgg tcttccccgt cctcaccagc caggtcgtgt
      301 cccgggctcg gaccgcggag cggggctcgg cgatgtccat cttcacctcg atcttcgaca
      361 tcgcgttgct gcttgcggcg ccgactgtgg gattcctgat cgatggcttc gactaccggc
      421 tggctttcgg tgcagtggcg atcgcactgg gcgccggcgc cgtggtgttt gcgatctggg
      481 accggcgaac cgtggtcgac cccgccgtcg cctgagtcag cgtcggtcgg cgaagaagcg
      541 ctccaggagc agggcgctct cctcggccag caccccatcg atcagctcga cccggtggtt
      601 cagacgtcgg tcctgtggga tgttgtacag cgaccaggcc gcccccgcct tgaggtcggc
      661 tgctccgtag acgatccgat cgagacgggc gagcaccgcg gcgccggcgc acatggcgca
      721 cggctcgagc gtcaccacca gcgtgtggcc ggtcagccgc cagtcgccca gctcgcgggc
      781 ccgggcggac atgaccagga tctcggcatg cgccgtggga tcggacagct cctcgcgtcg
      841 attgcggtcg accgtcacca gccggcccga gtcgtcgagc agcaccgccc cgatcggcac
      901 gtcgccactg gattcggcct cggcggcagc ccgcagcgcc tgtcgcatcg gttcgaggta
      961 gctgtcgggg gaagtgggat gagtcacggg tgtggattat cctttgtctg cctgctgggt
     1021 ccggtaggcg cttctggagg ggtcgcatag tctggtctag tgcgcggcac tggaaatgcc
     1081 gttgggggtt tacgctccct cgagggttca aatccctctc cctccgcgaa gtccagctgc
     1141 cgaaatcaca tctcacaact caactcggcc gagcctggct cggtgctcgg aggggtcgca
     1201 tagtccggtc tagtgcgcgg cactcgaaat gccgtagggg gctgacgctt cctcgagggt
     1261 tcgaatccct ccccctccgc ctgatggtgg gccgcgccgg ttgggtcgat ggcgctgtca
     1321 gccggcttcc gggggctcga atcggtatcc catccctggc tcggtcttga agtagcgcgg
     1381 gttgctcggc tgtggctcga gcttgcgtcg tagttgggcc atgaacaccc ggagatagtt
     1441 ggtctcctcg ccgtactgag ggccccacac ctctttcagg agttgggtct gggtgacgag
     1501 tctgccccga tgccggacca gcatctccag gatgccccac tcggtcggtg tgagtcggat
     1561 ctcctccccc gaggcaccgg tgaccacctt cttcgccagg tcgatagaga agtcatcggt
     1621 caccactgtc ggctgatcct ctcctgagtc ggactccctc cgcagcgccg cccgcagacg
     1681 cgccaacagc tcacccatcc cgaacggctt cgtgacgtag tcgtcggcgc ccgcatccag
     1741 ggcggcgatc ttctcccgct cggcgtcgcg tacagagagg acgatgatgg gcacgctgct
     1801 ccacccccgc agtccccgga tgacttcgat accgtcgatg cccgggagcc cgaggtcgag
     1861 gatcacggtg tcggggcgat ggtcggccgc cttgcgcagg gcctcttctc ccgacccggc
     1921 ggtgtcgacc cggtagccga gggccttcag gttgatggcg agggcccgca cgatctgcgg
     1981 ctcgtcgtcg acgaccagga tgcgtcgggc ttcagccatg agacagcccc gcatcctcga
     2041 cctcggtttc gatcacgccg gcgatcttca tggcgatgac catggtggtg ccacccccag
     2101 gagtgtcctc gatgatcaac tcgttcccca tcgcctcgag gagcccacgt gacactgcca
     2161 gacccagccc cacgccctca cttcgggaag agtcacccag ccgttggaat ggctggaaaa
     2221 cggtctctcg ggagtcgatg gggatcccgg ggcctcgatc gctgatacgt agatcgatcc
     2281 ggcgggcgat ctccccggcg ctga
//
LOCUS       DATITW010000661         1575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_352499, whole genome shotgun sequence.
ACCESSION   DATITW010000661 DATITW010000000
VERSION     DATITW010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352499"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VLS86_09240"
     CDS             complement(<1..252)
                     /locus_tag="VLS86_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50719.1"
                     /translation="MVVAPSYDSRGLVNLVAEVESRMIGSAPGPILDPAIGAMIPKGR
                     TYVMVMFDGLGVAQLQHVAAGSLLRSQRATLEAPFPTTTS"
     gene            374..739
                     /locus_tag="VLS86_09245"
     CDS             374..739
                     /locus_tag="VLS86_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSO50720.1"
                     /translation="MSQYFESYLTVGAFAALGAVLVMVMLGVATVIRPTNPTASKVLT
                     YECGVDPVGSGWSQSYVRYYVYGLLFVIFDVEAVFIFPWAIVLEDLGMFGLVEMLIFI
                     GILVLGLVYAIRKGVLRWE"
     gene            730..1182
                     /locus_tag="VLS86_09250"
     CDS             730..1182
                     /locus_tag="VLS86_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSO50721.1"
                     /translation="MGVVQRFGAPKPVSWLLNWSRKYSLWLFQFGLACCAIELMAASG
                     PRYDFMRFGIIPLPASPRQADLMVVAGTVTDKMAPAIRRLYDQMPDPKYVISMGSCAN
                     CGGPYWDSYSVTKGVDQIIPVDVYVPGCPPRPEALMDGIVLLQQKIKG"
     gene            1208..>1575
                     /locus_tag="VLS86_09255"
     CDS             1208..>1575
                     /locus_tag="VLS86_09255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSO50722.1"
                     /translation="MTSSPSDTDTAPHPLQGLADDVAAAVGGEALIAFDTVKVNVPPE
                     RWVAALTTARDDLGLVFFSWLSAVDWSAEVEVGDPPGDDVRERFELMAAVGDVSDGHR
                     VIFTTDLPKESPVAPSLVGV"
BASE COUNT          250 a          487 c          524 g          314 t
ORIGIN      
        1 gctggtggtg gtggggaacg gcgcttccag tgtcgcccgc tgcgatctga gcagggagcc
       61 ggcggcgaca tgctgcagct gggccacgcc gagcccgtcg aacatgacca tgacataggt
      121 ccgacccttg gggatcatcg ctccgattgc gggatcgagg atggggccgg gagcgctgcc
      181 gatcatgcgg gattcgacct ccgcgaccag gttgaccagg ccgcgggagt cgtatgacgg
      241 ggcgacgacc atcgtcgcag gctagggcgg gcgcagatgt ttttcccgat gggagttgaa
      301 gtttcctgac ttcgacggaa aacgacccgt gcgtatactc gccgccggtt ggtattcgtc
      361 gacccgggca ggcatgagcc aatactttga gagttatctg acagtcggcg ccttcgccgc
      421 acttggcgcg gtgctggtga tggtgatgct cggcgtggcg accgtgatcc ggcccaccaa
      481 cccgacggct tccaaggtct tgacctacga gtgcggggtc gacccggtgg ggtccggctg
      541 gagccagagc tacgtccgct attacgtgta cgggctcctg ttcgtcatct tcgacgtcga
      601 ggcagtcttc atcttcccct gggccatcgt cctcgaggat ctggggatgt ttggtctcgt
      661 ggagatgctg atcttcatcg ggatcctggt gctcgggctg gtctacgcca tccgcaaggg
      721 agtcctgcgg tgggagtagt ccagcggttc ggggccccga agccggtgag ctggcttctc
      781 aactggtccc ggaagtattc actctggttg ttccagttcg ggctcgcctg ctgtgcgatc
      841 gagctgatgg cggcctcggg gccacgatac gacttcatgc gctttggcat catcccgctc
      901 ccggcttctc cccggcaggc cgacctgatg gtcgtggccg gcaccgtgac cgacaagatg
      961 gcgcccgcca tccgccgtct ctatgaccag atgccagacc ccaagtacgt gatctccatg
     1021 ggctcctgtg ccaactgcgg cgggccctat tgggactcct actcggtgac caagggcgtc
     1081 gatcagatca tccccgtcga tgtgtacgtg cccggctgcc caccccgacc cgaggcgttg
     1141 atggacggga tcgtcctcct ccagcaaaag atcaagggct agaacatcaa agagaagtgg
     1201 aatcggcgtg acctcgagcc cgtctgatac cgacaccgcg cctcatcccc tccaggggct
     1261 ggccgacgac gtggccgcgg cggttggcgg tgaggcattg atcgctttcg acacggtgaa
     1321 agtcaatgtc ccgcccgagc gctgggtggc ggcgctgacc acggcgagag acgatctcgg
     1381 tctggtcttc ttctcatggc tgtcggcggt cgactggtcc gcggaggtag aagtcggcga
     1441 tccgcccggg gacgacgtcc gcgagcgttt cgagctcatg gccgcggtcg gcgacgtgag
     1501 cgacggccat cgggtcatct tcaccaccga tctccccaag gagtcaccgg tcgctcccag
     1561 cctcgtcggg gtcta
//
LOCUS       DATITW010000662         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_152389, whole genome shotgun sequence.
ACCESSION   DATITW010000662 DATITW010000000
VERSION     DATITW010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152389"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VLS86_09260"
     CDS             complement(<1..624)
                     /locus_tag="VLS86_09260"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO50723.1"
                     /translation="MRVDPATTRTLLHRDPQVQEVLDIWAARFLHGHIELGDLTATME
                     RIDDWTDWGPEWMKTARAHEELGEQALDEGRRISAVGFFVSAARCYHLSYFLSVDDEN
                     AHALGLAKMMECHDRVLPYMEPAVEKIAIPFPDADLAGLLSIPPGDGPYPVVIFLPGL
                     DSTKEQRHGGRGSLLRRGLAVLSLDGPGQGEIRAKLPIRHDSEVAVSA"
     gene            complement(621..1679)
                     /locus_tag="VLS86_09265"
     CDS             complement(621..1679)
                     /locus_tag="VLS86_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSO50724.1"
                     /translation="MTERVKLASIGLGRWARVLARGAQRGSVIELASCFSRSPERRSA
                     FQEEFGVPRAASTLDELLADPEIEGVLVTTPNDTHKAVILESLDAGKAVYTDKPIAHT
                     MEDAGEITRAAAASGVPFAVGHSARRLGGHRAMKHWVETGKIGRVSLAEANFSNERGL
                     ELTTDTWRWFADKSPGGAMIQLGVHHADNLQYLLGPVAAVSSIARKLYTKAEVPDAIM
                     SILEFESGPLGYLGTGWASPGVYQLRLQGTEANLFYDLDFNNWDESHLVDAKSTLVSQ
                     AYRESVRPPVDMPRTDMFREQLDEFALAIRGEADIEVGAVEALRALAVVHAALESSVR
                     KGEAVAVAPMVERAGALS"
     gene            complement(1676..2062)
                     /locus_tag="VLS86_09270"
     CDS             complement(1676..2062)
                     /locus_tag="VLS86_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSO50725.1"
                     /translation="MTVVGPDELRFRDFPGRTTSDPFKEAGQGASTVRQVVIDYVASR
                     SPHLHPQSEEIVYVVAGRGRVWLDGVFHSVGPGSWYRIPARTPHATMADRGERLSLVC
                     FFPHDDFPNNIQELDMVIEADKEGGT"
     gene            complement(2059..>2561)
                     /locus_tag="VLS86_09275"
     CDS             complement(2059..>2561)
                     /locus_tag="VLS86_09275"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HSO50726.1"
                     /translation="FESYLSNLCDEMVDLGAHTFVFVNWHEGNIASLDAVATDLQTDN
                     EVLVVTAQACYTAQRIYAPVGGELTHGGGIETLAVLAHDPQLAQIDLAGDPTRPEGAD
                     KLDAMRRSREIYGYITDVTEIADDGWYGNPAWADEGRATDFAKTVADEIAVTVESILK
                     LRRGRP"
BASE COUNT          433 a          834 c          842 g          452 t
ORIGIN      
        1 ggccgagacc gcgacctcgg agtcgtggcg gatcggcagc ttcgctctga tctccccctg
       61 gcccggaccg tcgaggctga gcacggccag cccgcggcga agcaacgacc cccgcccgcc
      121 gtggcgttgc tccttggtcg agtccaaacc ggggaggaag atcaccacgg ggtacggacc
      181 gtcacccggg ggaatgctga gcagaccggc gaggtcggcg tcggggaacg ggatggcgat
      241 cttctccacc gccggctcca tgtagggcag aacccggtca tggcactcca tcatcttggc
      301 caggccgagg gcatgagcgt tctcgtcgtc gacggagagg aagtaggaga ggtgatagca
      361 ccgcgccgcc gacacgaaga agccgaccgc ggagatgcga cgaccctcgt ccaacgcctg
      421 ctcgcccagc tcctcgtggg cgcgggcggt cttcatccat tccgggcccc aatcggtcca
      481 gtcgtcgatc ctctccatgg tcgccgtaag gtcgccgagt tcgatgtgac cgtggaggaa
      541 ccgggccgcc caaatgtcga ggacctcctg gacctgtgga tctcgatgga gcagggtgcg
      601 ggtggtagcg gggtcgactc tcatgacagc gctcccgccc tctccaccat cggagcgact
      661 gcgacggcct cgcctttccg tacggaggac tccaatgcgg catgaaccac ggcgagggcc
      721 cggagggcct caacggcacc gacctcgatg tcagcttctc ctctaatggc gagtgcgaac
      781 tcgtcgagct gctcccggaa catgtcggtg cggggcatgt ccactggagg cctgacggac
      841 tcccgatagg cctggctgac cagcgtgctc ttggcgtcga ccagatggga ctcgtcccag
      901 ttgttgaagt cgaggtcgta gaaaagattc gcctcggtcc cctggaggcg gagctggtag
      961 acccccggcg aggcccaacc ggtgccgagg tatccgaggg ggcccgactc gaactcgagt
     1021 atcgacatga tggcgtcggg cacctcggcc ttggtataga gcttgcgggc gatgctggag
     1081 acggcggcca ccggcccgag cagatactgg aggttgtcgg cgtggtgcac ccccagctgg
     1141 atcatggccc cgcccgggct tttgtcggca aaccagcgcc acgtgtcggt ggtcagttcg
     1201 agcccgcgtt cgttcgagaa gttcgcctcg gccagggaga cccggccgat cttgccggtc
     1261 tcaacccaat gcttcattgc gcgatggcca ccgagacgcc gggcgctgtg gcccaccgcg
     1321 aatggcaccc ccgaagcggc cgccgcccgg gtgatctccc cggcgtcctc catcgtgtgc
     1381 gctatcggct tgtcggtgta gacggccttg ccggcgtcga ggctctccaa gatcacagct
     1441 ttatgcgtgt cgttgggcgt cgtcaccagc accccttcaa tctccgggtc ggccagcagc
     1501 tcgtcgagtg tcgaggcggc cctgggaacg ccgaactcct cctgaaacgc ggagcggcgc
     1561 tcgggagagc gcgagaagca actcgccagc tcgatcaccg atcccctctg cgcgcctcgg
     1621 gccagcaccc tcgcccagcg gcccagcccg atcgatgcga gtttgacccg ttcagtcaag
     1681 ttccgccctc cttgtcagcc tcgatcacca tgtcgagctc ctggatgttg ttgggaaaat
     1741 cgtcgtgggg aaagaagcag acgagcgaca gacgctcgcc gcggtcggcc atggtggcgt
     1801 gcggtgtcct cgccgggatt cgataccacg atccaggccc gacagagtgg aagacgccat
     1861 cgagccaaac ccggcctctt cccgcgacga cgtacacgat ctcctcgctc tgcggatgca
     1921 aatgaggaga cctggaggcg acgtaatcga tcaccacctg tcgcacggtt gacgcaccct
     1981 gcccggcttc cttgaacggg tcggaggtcg tgcgtccggg gaagtcgcga aaccggagct
     2041 cgtccgggcc gacgacggtc atggtctccc tcgcctcaac ttgaggatcg attccacggt
     2101 gacggctatc tcgtcggcca ccgtcttggc gaaatccgtc gctcttccct cgtcagccca
     2161 ggccgggttg ccgtaccagc catcgtcggc gatctcggtg acatcggtta tgtagccgta
     2221 aatctcgcgg gagcgccgca tggcgtcgag cttgtcggct ccttctggcc gggtcgggtc
     2281 gccagccagg tcgatctgcg ccagctgggg atcatgggcg aggacggcga gcgtctcgat
     2341 cccgccaccg tgagtgagtt ctcctccgac gggggcgtag atgcgttgcg ccgtgtaaca
     2401 cgcttgagcg gtgaccacga ggacctcgtt atctgtctgt aggtcggtgg ccaccgcgtc
     2461 gagggaggcg atgttgccct catgccaatt gacgaagacg aaggtgtggg cgcccaggtc
     2521 caccatctca tcgcagaggt tggagagata tgactcgaaa a
//
LOCUS       DATITW010000663         1697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_512907, whole genome shotgun sequence.
ACCESSION   DATITW010000663 DATITW010000000
VERSION     DATITW010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512907"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VLS86_09280"
     CDS             complement(<1..815)
                     /locus_tag="VLS86_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HSO50727.1"
                     /translation="MAETLLSFAGQDMAIDLGTANTLVYVRGGGIVLNEPSVVAINSQ
                     NGRALAVGMEAKRMIGRTPAHITAIRPLRDGVIADFDVTEKMLRYFIQKVHRRKWARP
                     RIVVCVPSGITPVERRAVEEAAYHAGARRAYTIEEPMAAAIGSGLPIGEPSGSMVVDI
                     GGGTTEVAVISLGGIVVSRSIRVGGDELDSAIIDWIKKEHNVLVGERTAEQLKIAVGS
                     AWPYSDEPSAEVRGRDLVTGLPKTILLSSQEVREAIEEPTQAIVDAVKYTLDKT"
     gene            complement(938..1342)
                     /gene="ndk"
                     /locus_tag="VLS86_09285"
     CDS             complement(938..1342)
                     /gene="ndk"
                     /locus_tag="VLS86_09285"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HSO50728.1"
                     /translation="MAIENTFIMVKPDGVERGLVGEVIGRLERKGLILEKIRQLRIDD
                     ALARRHYEEHVEKPFFPELLAFITSGPVVAMEWSGESAISVCRGLMGATDPKQAAPGT
                     IRGDLGLMVTQNIVHGSDGAESAQRELEIYFG"
     gene            complement(1392..>1697)
                     /locus_tag="VLS86_09290"
     CDS             complement(1392..>1697)
                     /locus_tag="VLS86_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HSO50729.1"
                     /translation="EYPTTRWQLVLGVMGDKNVESMVESLAHLIEGIVVTAPKSERAV
                     DPVTLAERVRPLVDVPVLVADEVALALDMARAEAGSQGAVLVAGSLYLVGEVRELLT"
BASE COUNT          294 a          580 c          511 g          312 t
ORIGIN      
        1 gtcttgtcga gtgtgtactt gactgcgtcg acgatggcct gagtcggctc ctcgatggcc
       61 tcccggacct cctgggatga cagcaggatc gtcttgggca gtccggtgac tagatctcgt
      121 ccgcggacct cggcggaggg ctcatccgaa tagggccagg ccgatccgac ggcgatcttg
      181 agctgttcgg cggtccgctc accaacgagc acgttgtgct ccttcttgat ccagtcgatg
      241 attgcggagt cgagctcgtc gccgccgacc ctgatcgacc gggacaccac gatgccaccg
      301 agagagatca cagccacctc tgtggtgcca ccaccgatgt cgaccaccat cgagcctgaa
      361 ggctcaccaa tggggagacc cgacccgatt gccgccgcca tcggctcttc gatggtgtag
      421 gcgcgacggg cgccggcgtg ataggcggcc tcctcgactg cccttctctc caccggggtg
      481 atccccgaag gcacacaaac gacgatccgg ggccgcgccc actttctgcg gtggaccttc
      541 tggatgaaat agcggagcat cttctctgtc acgtcgaagt cggcgatgac accgtcgcgg
      601 aggggccgga tcgcggtgat gtgggccggg gtgcggccga tcattcgctt tgcctccatg
      661 cccacggcca gtgcccgacc gttctgggag ttgatcgcaa ccacggaggg ctcgttgagc
      721 acgatgccac caccccggac gtagaccagg gtgttcgccg tgccaaggtc gatggccata
      781 tcctggccgg cgaaagaaag aagggtttca gccatggctg atggagtctc ccgggatgac
      841 ccgcgaagtg tagggggacg cccgatgttc tgccatagcg atctccaccc tatccgccct
      901 cagggcaaac cgagatcggg gcaccgatga cggcgaatca gccgaagtag atctccagct
      961 cacgctgggc gctctccgcg ccgtctgagc catggacgat gttctgggtg accatcaggc
     1021 cgagatcccc acggatcgtg cccggcgccg cctgtttggg atcggtggcg cccatcaacc
     1081 ccctgcacac cgagatcgcg gactcccccg accactccat ggccacgacc gggcctgagg
     1141 ttatgaatgc gagcagctcg gggaagaacg gcttctccac gtgctcctcg tagtggcgtc
     1201 gggccagagc atcgtcgatc cgcagctgcc tgatcttctc caggatgagt cccttgcgct
     1261 ccaggcgccc gatcacctcg ccgaccagac cacgttcgac accatcgggt ttgaccatga
     1321 tgaaggtgtt ctcgattgcc atctcgctgc aggctactgc ctggatcctg gccgacttcg
     1381 tgctcgaagg ctcatgtcag aagctcgcgc acctctccca ccagatacag cgagccggcc
     1441 accaggaccg cgccctggct cccggcctcc gccctggcca tgtccagggc cagggcgact
     1501 tcatcggcga ccaggaccgg cacgtcgacc agtggtctga ccctctcggc caaggtcact
     1561 gggtcgacgg cccgctccga tttcggggca gtgaccacaa tcccctcgat caggtgcgcc
     1621 aggctctcca ccatcgactc cacgttcttg tcgcccatca cccccaacac cagctgccat
     1681 ctggtggtcg ggtactc
//
LOCUS       DATITW010000664         2891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_633773, whole genome shotgun sequence.
ACCESSION   DATITW010000664 DATITW010000000
VERSION     DATITW010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633773"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VLS86_09295"
     CDS             <1..301
                     /locus_tag="VLS86_09295"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HSO50730.1"
                     /translation="AERPGFVNSHPMLHSRWWPAIESDGSDALDELVTMSGYGGEVGD
                     IWSGTAEVDLSESPSEELSLLHPIEIVAGYYHRVGVSWGGGTTLRRTFPMEVEGI"
     gene            298..1752
                     /locus_tag="VLS86_09300"
     CDS             298..1752
                     /locus_tag="VLS86_09300"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HSO50731.1"
                     /translation="MTAILDELKKQGKTGPFIGGSWSHGAGELAVVDPATEDVLVDIG
                     LAEASDAEDAVTAAYEALAPWGATPPRERGEILRRTFEAMRAREEDLAELIVLENGKA
                     FPDALGEVRYAAEFFRWFSEEASRIGGELRLAPGGDKRIITVRQPVGVSVMITPWNFP
                     AAMATRKIGPALAAGCTAIVKPASDTPLTTLAVVHLMEQAGLPAGVVNVVVAKGSSGV
                     VEQMLHDPRVRKLSFTGSTEVGQTLLKVAADQVLNVSMELGGNAPFIVFEDADLEAAL
                     EGAMVAKMRNAGEACTAANRFLVHSSVVDEFTSMLAGAMGAMKVGPGIDRSTQVGPMI
                     NQRSRDDIAGMVQAAVDSGAGVAVGANVPDRRGFFYAPTVLSGVSPDSAILDQEIFGP
                     VAPVVTFDSDDEAIAMANDTVHGLISYVYTTDLARGLRTAEAIESGMVGLNRGLVSDP
                     AAPFGGVKQSGIGREGSHEGIEEFLETKYIATSW"
     gene            complement(1772..2338)
                     /locus_tag="VLS86_09305"
     CDS             complement(1772..2338)
                     /locus_tag="VLS86_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50732.1"
                     /translation="MALVGPEPWLILSTEDYPGTDCVAVGVHQDLQIWNKGTESVTVE
                     WLGANVAIGSDDKHETGPVGEVLDPGTNVLEASPYEAPILHVVDPDDSASAQTEMTLT
                     GFGGIQLGMTVAEAADSFGHPITVDPDLAPGPECWQAVVAGDPYSPILTVEGEGNADS
                     VVIAITTFYPPDAELTISDPRAAAALCP"
     gene            complement(2533..>2891)
                     /locus_tag="VLS86_09310"
     CDS             complement(2533..>2891)
                     /locus_tag="VLS86_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50733.1"
                     /translation="LEQGGLVRTAQSTTDQAALFYAFLAMENARDVVYAFVDTVEKAH
                     RVASDIHLQGPPGVLLGFNESKRGEGYVIVDASVDIAQIVREVTGEDPVRVLTQDQIT
                     AEPDMWGTQVVRADEL"
BASE COUNT          515 a          908 c          952 g          516 t
ORIGIN      
        1 ggccgagagg cccggcttcg tgaactcgca tcccatgctc cacagccgat ggtggccggc
       61 aatcgagtcg gacgggagcg atgcactcga tgagctggtc acgatgagtg gatatggcgg
      121 ggaggttggc gacatctggt cgggaacggc cgaggtcgac ctttccgagt caccgagtga
      181 ggagctttcg ctgctccatc caatcgagat cgtcgccggc tactaccacc gggttggcgt
      241 ctcctggggc ggcggcacca cactgcgcag gacattccca atggaggttg aaggcatatg
      301 acggcgatcc tggacgagct caagaaacag ggaaagaccg gccccttcat tggcggctcc
      361 tggtcgcacg gagcggggga gctggcggtc gtcgacccgg ccaccgagga cgtgctggtc
      421 gacatcggcc tcgctgaggc cagcgacgcc gaggacgcgg tcaccgccgc ctacgaggcc
      481 ctggctccct ggggagccac tccgccacgg gagcgtggcg agatcctgcg tcgcacgttc
      541 gaggcgatgc gggcccgtga agaggacctg gcggagttga tcgtcctcga gaacggcaag
      601 gcgttccccg acgctctggg cgaggttcgt tacgcggccg agttcttcag gtggttttcc
      661 gaggaggcgt cccgaatcgg gggagagctc cgtctggcgc caggcgggga caagcggatc
      721 atcaccgtgc gtcagccggt cggggtgagc gtgatgatca cgccgtggaa cttcccggcc
      781 gccatggcaa cccgcaagat cggccccgcc ctcgccgcgg gctgcaccgc catcgtcaag
      841 ccggcatcgg acacgcccct caccacgctg gcggtggtcc acctgatgga gcaagccggg
      901 cttcccgccg gggtggtcaa cgtcgtcgtc gccaaaggct catccggcgt cgtcgagcag
      961 atgctccatg accctcgtgt caggaagctc tctttcaccg ggtcgaccga ggttggccag
     1021 acactgctca aggtggcggc cgaccaggtg ttgaacgtct ccatggagtt ggggggcaac
     1081 gccccgttca tcgtcttcga ggacgccgac ctcgaagccg ctctcgaagg agccatggtg
     1141 gccaagatgc gcaacgcggg ggaggcgtgc accgcagcca accgcttcct ggtccactcc
     1201 tcggtggtcg acgagttcac ctcgatgctg gccggcgcca tgggggcgat gaaggtcggg
     1261 ccgggcatcg atcggagcac ccaggtcggc ccgatgatca accagaggtc gcgagacgac
     1321 atcgcgggca tggtccaagc cgcggtggat tcgggggccg gtgtcgccgt cggggccaac
     1381 gttcccgatc gtcgtgggtt cttctacgca ccgaccgtgc tctccggagt ctctcccgac
     1441 tcggcaatcc tcgaccagga gatcttcggc ccggtcgctc ccgtcgtcac cttcgactca
     1501 gacgacgagg caatcgccat ggccaacgac acggtgcacg gattgatcag ctacgtgtac
     1561 accaccgacc tggcccgcgg attgcgaacc gcagaggcga tcgaatcggg catggtcggg
     1621 ttgaaccggg gtctggtgtc cgatccggcc gcacctttcg gcggggtcaa gcagtcgggc
     1681 atcggccgtg aggggagtca cgaggggatc gaggagtttc tcgagaccaa gtacatcgca
     1741 accagttggt gagctcaggc cggcgccctg ctcacgggca gagtgccgcg gccgcacgag
     1801 gatcgctgat ggtcaattca gcgtctggtg gatagaacgt ggtgatggcg atgacgacgc
     1861 tgtccgcgtt tccctcgccc tcgacggtga gaatcggtga gtaggggtca cctgcgacga
     1921 ccgcttgcca acattcgggt cccggagcca gatcgggatc caccgtgatc ggatgtccga
     1981 atgagtcggc tgcctccgcg accgtcatgc ccagttggat gccgccgaac cctgtgagcg
     2041 tcatctcggt ctgcgcactg gcactgtcgt cggggtcgac cacatgaagg atgggtgctt
     2101 cataaggact cgcctccagc acgttggttc ccgggtcgag gacctcgccg actggcccgg
     2161 tctcatgttt gtcgtcggat ccgatcgcga cgttcgctcc cagccattcc accgtgaccg
     2221 actcggttcc cttgttccat atctggagat cctgatggac accgaccgcg acgcaatctg
     2281 tgcccggata gtcctcggtg gacaggatca gccaaggttc tggtcctacc agtgccacac
     2341 acgcgtcctc tgctgccgaa gaggtgacgg cagtagaatc agagcaagaa ccaacggcca
     2401 cgccgaacag gacaatgagc gcggccggcg atctcctcat caccaacaga cggccggaag
     2461 aggccgaaag ttccatttcg agggcactga cgagtctctc aactgcggtt tctgacggag
     2521 ggggcgccgc ggtcacagtt catcggcgcg tacgacctga gtgccccaca tgtccggctc
     2581 cgcggtgatt tggtcttgcg tcaggacacg gactggatcc tccccggtga cttcccgaac
     2641 gatctgggcg atgtcgaccg aggcgtccac aattacgtag ccctcgcctc gcttcgattc
     2701 gttgaatccg agtaagaccc cgggtgggcc ttgcaggtgg atatcgctcg caacccgatg
     2761 tgctttctcg accgtgtcta caaatgcata gacaacatct cgagcattct ccatggcgag
     2821 gaaagcgtag aaaagtgcag cctggtccgt cgtcgactga gcggttctga caagcccgcc
     2881 ctgctccagg g
//
LOCUS       DATITW010000665         1692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_272851, whole genome shotgun sequence.
ACCESSION   DATITW010000665 DATITW010000000
VERSION     DATITW010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272851"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VLS86_09315"
     CDS             <1..720
                     /locus_tag="VLS86_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSO50734.1"
                     /translation="LKFTIGYSQRFNSKQALVKRSLGDGTLGDPVSVLISRHITRSLG
                     AKIGSRTKLSPAAMEATHDIDFSLWCLEPRRPVRVYSQVAWGARRGDGVPDSQVMIIT
                     MDDGVTVTIGAGWSLPPGYPNFSATWIEFVGTDGALMMDASHRDIVLNTVKDGVQFPL
                     STMPGEYVDHVYAGPMERETIHFLEAVAYDRPVMVEPRLARQTMEVYIAADLSVETNQ
                     VVELPLVPDSAQQSLLSAVKS"
     gene            complement(710..>1692)
                     /locus_tag="VLS86_09320"
     CDS             complement(710..>1692)
                     /locus_tag="VLS86_09320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSO50735.1"
                     /translation="NPAGRLVARVGPGRSLRIGGLLTLTACVVAGTASTWWVLPIATA
                     VGGAGNGLIQVASNLAIFDGVALRRQGLGFGAKQASVPMASVLAGISLPLIGLVFGWR
                     WVFAIAALIALALAVSAPRLDMARAAERNEGRIGRPPLSLLLLSLGGITGAMAGNGLS
                     LFIVPSAVDIGISEGAAGAVLAACSFLVVAVRLGAGWTVDRSESHGHVEMAWLAGAGA
                     VGGLVLLLSSAPGLYLVSMPVAVLGAWGWPGIFFYTVVHSYPDFPARASGLALSGNLT
                     GTVIGPLVVGSLAGRANYPGAWLFVTLMSAISAIAFVASYRTKARTPVSS"
BASE COUNT          310 a          591 c          564 g          227 t
ORIGIN      
        1 ctcaagttca ccattggcta ctcgcagcgc ttcaactcca agcaggccct ggtgaagcgg
       61 agtctcggcg acggcacctt gggcgacccg gtgagcgtgc tcatcagccg gcacatcacc
      121 cgttcgctgg gggccaagat cgggtcgcgc accaagctgt caccggcggc catggaagca
      181 acccacgaca tcgacttctc gctctggtgt ctggagccgc ggagaccggt gcgggtctac
      241 tcccaggtgg cgtggggagc gagaaggggc gacggggtcc ccgacagcca ggtgatgatc
      301 atcaccatgg acgacggggt gacggtcacc atcggggccg gctggtcgct tccccccgga
      361 taccccaact tctctgccac ctggatcgag ttcgtgggga ccgacggggc actgatgatg
      421 gatgccagtc accgggacat cgtcctcaac acggtcaagg acggggttca gttcccgttg
      481 tcgacgatgc cgggcgagta cgtggaccac gtgtacgccg gtcccatgga gcgggagacg
      541 attcacttcc tggaagcagt ggcctatgac cggccggtga tggtcgagcc gagactcgcc
      601 cggcagacga tggaggtcta tatcgcggcc gatctctcgg tggagaccaa ccaggtggtg
      661 gagctgcccc tggtccccga ctcggcgcag cagagcctgc tgagcgcggt caagagctga
      721 ccggggtcct cgccttggtc cggtaggagg cgacgaaggc gatggccgat atcgccgaca
      781 tcagggtcac gaacagccac gcccccgggt agttggctcg ccccgccagc gatccgacga
      841 cgaggggccc gatcaccgtc ccggtgagat tgccggagag ggccaagccc gaggcgcggg
      901 ccgggaagtc gggatagctg tgcacgaccg tgtagaagaa gatcccgggc catccccacg
      961 cccccagaac cgcgacgggc atcgacacca ggtagaggcc aggcgccgag gacagcaaca
     1021 gcacgagccc acccaccgcc ccggctcccg ccagccaggc catctcgacg tggccgtggc
     1081 tctcgctccg atcgaccgtc catccggcac cgagtctcac cgccaccacc aggaacgagc
     1141 acgccgccag caccgccccg gccgcaccct cgctgatgcc gatatccacg gccgagggga
     1201 cgatgaacaa ggagagcccg ttgcctgcca tggcccccgt gatcccgccg agagacagca
     1261 acagcaagga cagcggcggg cggccgatcc gcccttcgtt tctctccgcg gcccgggcca
     1321 tgtcgagccg gggtgccgag accgcgagag cgagggcgat gagcgcggca atggcgaaca
     1381 cccagcgcca cccgaagacc aagccgatca agggcaggga gatccctgcc aggacgctcg
     1441 ccatggggac cgaggcctgt ttcgccccaa agccgagacc ctggcgacgc agggccacgc
     1501 cgtcgaagat cgccaggttc gatgccacct ggatcaaccc gttgcccgcg cctccgaccg
     1561 cggtggcgat gggtagcacc caccaggtgc tcgccgtccc ggcgacgaca catgcggtga
     1621 gtgtgagcag ccccccgatg cggaggctcc tccccggtcc caccctggcc accagccggc
     1681 ccgccgggtt gg
//
LOCUS       DATITW010000666         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_673146, whole genome shotgun sequence.
ACCESSION   DATITW010000666 DATITW010000000
VERSION     DATITW010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673146"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VLS86_09325"
     CDS             complement(<1..853)
                     /locus_tag="VLS86_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSO50736.1"
                     /translation="MSRTVIKGGTVVTATEQSHSDVLIDGEKVAALAAPGSHNWESGA
                     DKVIDANGRYVMPGGIDVHTHMELPFGGTFAADTFETGTRAAAWGGTTTIIDFAVQAK
                     GQNLMDGLEAWMGKAEGNCAIDYGFHLIVGDVTDQSLKEMDLAIEQGVTSFKMFMAYP
                     GVFYSTDGEIFRAMQKAAENGSTIMMHAENGIAIDILIAQALARGETDPIHHGLTRPS
                     VLEGEATHRAIKLAEVAGCPVYIVHMSAAEALIELTMARDEGQNAFGETCPQYLFLSL
                     DHLGNGFE"
     gene            complement(863..1705)
                     /locus_tag="VLS86_09330"
     CDS             complement(863..1705)
                     /locus_tag="VLS86_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HSO50737.1"
                     /translation="MPNVVRAAIVQTEWTGDKDSMIKKNADYAREAAEQGAQVMCFQE
                     LFYGPYFCQVQENEHFDYAEPIPDGPTTKLMQDLAKETGMVLVVPIFEMEQEGLYYNT
                     AAVIDSDGTYLGKYRKNHIPHVKGFWEKFYFRPGNMGYPVFDTAVGKVGVYICYDRHF
                     PEGWRALGLNGAKIVFNPSATSRGLSMYLWKLEQPASAVANEYFIGAINRVGKEPFGD
                     NDFYGSSYFVDPRGQIIGDSASDTEEELVIRDLDLDQIEEVRQQWAFYRDRRPDAYGD
                     LVAP"
     gene            complement(1820..>2449)
                     /locus_tag="VLS86_09335"
     CDS             complement(1820..>2449)
                     /locus_tag="VLS86_09335"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSO50738.1"
                     /translation="AGITDEVEVVAQDFTMNALLNGEIDAAEAMIYNEYAQVLEAVNP
                     DTGELYQPEDLNVIDFNEVGTAMLQDAVWVDADWIAEEGNEDVAIRFLTATFQGWIHC
                     LDNFDECVEVVLNNGSTLGQSHQEWQLNEILALIFPATNGIGVMNADLWDQTVTVATE
                     QIAELQGVEISGDAYRTDLAEAAIERLGDADVTGEVYERREIELQEGGS"
BASE COUNT          412 a          798 c          746 g          493 t
ORIGIN      
        1 cctcgaaccc gttgccgagg tggtcgagtg acaggaacag gtactgggga caggtctccc
       61 cgaatgcgtt ctgcccttcg tcccgggcca tggtcagctc gatcagcgcc tcggcagccg
      121 acatgtggac gatgtagacc gggcaacccg ccacctctgc cagcttgatc gcccggtgcg
      181 tggcctcgcc ctcgagcacc gaggggcggg tcagtccgtg atgaatcggg tcggtctcgc
      241 ctcgggccag ggcctgggcg atgaggatgt cgatggcgat gccgttctcc gcgtgcatca
      301 tgatcgtcga cccgttctcg gccgccttct gcatggcgcg gaagatctcg ccgtcggtgg
      361 agtagaagac acccgggtag gccatgaaca tcttgaacga ggtgaccccc tgctcgatgg
      421 cgagatccat ctccttcaac gactggtcgg tgacgtcacc gacgatgaga tggaacccgt
      481 agtcgatggc gcagttcccc tccgccttgc ccatccaggc ctccaggccg tccatgaggt
      541 tctgaccctt ggcctgcacc gcaaagtcga tgatggttgt ggtgccgccc caggcggctg
      601 ccctggtccc ggtctcgaag gtgtcggcgg caaaggtgcc gccaaagggc aactccatgt
      661 gggtgtggac gtcgattcct cccggcatca catatcgacc gttggcgtcg atcaccttgt
      721 cggcgcccga ctcccagtta tggctcccgg gagcggccag ggcggccacc ttctccccgt
      781 cgatcaggac gtcggagtgc gattgctcgg tggcggtgac caccgtccct cccttgataa
      841 cggtgcgtga catcgcccct cctcagggtg ctacgagatc gccgtaggcg tccgggcgcc
      901 ggtcccggta gaaggcccac tgctgtcgga cctcttcgat ctgatcgaga tcgaggtcgc
      961 ggatcaccaa ctcctcttcg gtgtcggaag ccgagtcgcc gatgatctga ccccgggggt
     1021 cgacgaagta ggacgacccg tagaagtcgt tgtcaccgaa tggctccttg cccacccggt
     1081 tgatggcgcc gatgaagtac tcgttcgcca ccgcggaggc cggctgctcc agcttccaca
     1141 ggtacatgga gagcccgcgg ctggtggctg acgggttgaa cacgatcttg gcgccgttga
     1201 gccccagggc tcgccacccc tcgggaaagt ggcggtcgta acagatatag accccgacct
     1261 tgcccaccgc ggtgtcgaag accggatagc ccatgttccc cgggcgaaag tagaacttct
     1321 cccagaagcc cttcacatgc ggaatgtggt tcttccggta cttgccgaga taggtgccgt
     1381 cggagtcgat gaccgcagcg gtgttgtagt agagcccctc ctgctccatc tcgaagatgg
     1441 gcaccaccag caccatcccg gtctccttgg ccaggtcctg catcagcttg gtggtggggc
     1501 cgtcgggtat cggctcggcg tagtcgaaat gctcgttctc ctggacctga cagaaatagg
     1561 ggccgtagaa caattcctgg aagcacatga cctgggcgcc ctgctccgcg gcctcacgtg
     1621 catagtcggc gttcttcttg atcatcgagt ccttgtcgcc cgtccactcg gtttggacga
     1681 tggcggccct gacgacgttc ggcattggtc cctcctgaag acgatcgatc acaaacctag
     1741 cgtgtgcatc cacccaccat cacggcggga atctgcacac gctggtctgc atcgagccct
     1801 ggcgcgggca cccggttggc tagctgccgc cctcctggag ctcgatctcg cggcgctcgt
     1861 acacttcacc ggtcacatct gcatctccca accgctcgat cgcggcctcg gccagatcgg
     1921 tgcgataggc gtcgccggag atctccactc cctgcaactc cgcgatctgc tcggtggcca
     1981 cagtcacggt ctggtcccac agatcggcgt tcatgacacc tatcccattg gtggcgggga
     2041 agatgagggc gagaatctcg ttgagctgcc attcctgatg gctctgaccc agagtggagc
     2101 cgttgttgag aacgacctcg acacattcgt cgaagttgtc cagacagtgg atccagccct
     2161 ggaacgtggc ggtcaggaac cggatcgcaa cgtcctcgtt gccctcttcg gcgatccaat
     2221 cggcatcgac ccacaccgca tcctggagca tcgccgtgcc gacctcgttg aagtcgatga
     2281 cgttgagatc ctctggctgg tacagctccc cggtgtcggg gttgaccgcc tccagcacct
     2341 gggcgtactc gttgtagatc atcgcctcgg cagcatcgat ctcgccgttg agcagggcgt
     2401 tcatcgtgaa gtcctgggcg accacctcca cctcgtcggt gatccctgc
//
LOCUS       DATITW010000667         1587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_32924, whole genome shotgun sequence.
ACCESSION   DATITW010000667 DATITW010000000
VERSION     DATITW010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32924"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VLS86_09340"
     CDS             complement(<1..351)
                     /locus_tag="VLS86_09340"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HSO50739.1"
                     /translation="MSSDSARRERFEVISAEVFDPLQRYLRRRASPDDAGDLLSDVLL
                     TIWRRLDDVPAQAPLPWCYGVARRTLSNHRRSTRRRLRLVERIESEPIPPMFLDPAES
                     LQDPDLAQALAGLPI"
     gene            470..625
                     /locus_tag="VLS86_09345"
     CDS             470..625
                     /locus_tag="VLS86_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50740.1"
                     /translation="METDREKADRQETERKEEGARHERERKEEAAKYEEERKDQHERD
                     EQERRDS"
     gene            837..>1587
                     /locus_tag="VLS86_09350"
     CDS             837..>1587
                     /locus_tag="VLS86_09350"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HSO50741.1"
                     /translation="MGERTLDTPALNRALLSRQLLLRRHNLSIPQAVERIAGLQTQYA
                     PSAYIGLWSRLQGFRRSRLTHALEQGRVIQGTLMRVTIHIVSAGDYPLLTEAVRRPRR
                     EWWLRVGKDRALDEAGHGRVAAILRRALEDGPRSRSELIEILEAHGFSKGIWEGAGLW
                     IDMIRVPPSGTWERRRADLYGLAPTASVTESEGLQHLVRRYLGGFGPAGPGDIADWGG
                     VPLTSLKPVIESMTLRRFRDSGGGQLIDLPRL"
BASE COUNT          294 a          512 c          541 g          240 t
ORIGIN      
        1 gatgggaagc ccggccagcg cctgggcgag atccgggtcc tgcaacgact cggcggggtc
       61 aaggaacatg ggcgggatcg gctcggactc gatccgctcc acgagacgga gccgccgtcg
      121 cgtcgagcgt ctgtgattgg acagcgtgcg tcgggcgacg ccgtagcacc agggaagggg
      181 tgcctgggca gggacgtcat cgagacgtcg ccaaatggtg agcaggacgt cgctgaggag
      241 gtctccggcg tcgtcggggg aggcccgacg acgcagatag cgctgcagag ggtcgaacac
      301 ctccgctgag atcacctcga acctctcacg ccgcgctgaa tcggagctca cgtgtctgtt
      361 gtgtccggca cggggatgat tctgtcacct tcgacggcga cgccgccgac tcggagcgtc
      421 gaccagaacc gctgtaccct gggctggaga aaccaatatg gagtgcgctg tggaaaccga
      481 tcgggaaaag gcagatcgtc aggagacgga acgcaaagaa gagggtgccc gtcacgagcg
      541 tgagcgcaag gaagaggccg cgaagtacga agaggagcgc aaggaccagc acgagcgcga
      601 cgagcaggaa cgacgcgata gctgaaccgt gacggggggc ctgggtccgc cgacccagtt
      661 caatgcggga cgaccgacct atgcagcttt ttggcacccg ggtcgatcca ttcaccagca
      721 gcaaggccaa gtgccaagcg atcggctccc caccgatcga gcctccctga gaagcaggtg
      781 cggtcccacc atcccgcacc tgcttcgccg tatgggggtc ttagcctgac ccgatcatgg
      841 gcgagagaac cctcgacacc ccggccctga accgggccct gctctcccgt caactgctgc
      901 tccggcgcca caacctgtcg atcccccaag ccgtggagcg catcgccggg ctccagaccc
      961 aatatgcgcc ttctgcgtac atcggcttgt ggtcacgtct ccaggggttt cggcgttccc
     1021 ggctcaccca cgccctggag caggggcggg tgatccaggg aactctgatg agggtcacca
     1081 tccacatcgt ctccgcaggc gactacccgt tgctcaccga agcagtgcgc cggccgagac
     1141 gggagtggtg gctccgggtg ggcaaggatc gggctctcga tgaggccggc catggtcgag
     1201 tggccgcaat cctgaggaga gccctcgagg acggcccacg ctccaggagc gaactcatcg
     1261 agattctcga ggcccacggg ttcagcaagg ggatctggga aggcgccggt ctttggatcg
     1321 acatgatccg ggtccccccc tcggggacct gggagcggcg ccgagccgac ctctacgggc
     1381 tggcgccgac cgcctcggtg accgagtcgg aagggctcca acatctcgtg cgccgctatc
     1441 tcggtggctt cggccccgcc ggccccggcg acatcgccga ctggggtggc gtgccgttga
     1501 cgagcctgaa accggtgatc gagtcgatga ccctgcgccg attccgcgac agcggcggtg
     1561 gacagctgat cgatcttccc cgcctcc
//
LOCUS       DATITW010000668         1542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_352796, whole genome shotgun sequence.
ACCESSION   DATITW010000668 DATITW010000000
VERSION     DATITW010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352796"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..>1542
                     /gene="pknB"
                     /locus_tag="VLS86_09355"
     CDS             70..>1542
                     /gene="pknB"
                     /locus_tag="VLS86_09355"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HSO50742.1"
                     /translation="MESQTALTDRYVISSHLARGGMADVYEGMDTLLNRRVAIKVLHS
                     QYSADEAFVKRFRREAQAAANLSHPNIVGIYDWGQAQNTYFIVMELVDGRSLRDVLRA
                     EGALLARRATEITAEVAAALSVAHQAGLVHRDVKPGNILLAKDGTVKVTDFGIARAWD
                     DSAELTKTGAVIGTATYFSPEQAQGAGADARSDVYALGVVLYEMLTGRPPFTGDSPMS
                     VAFQHVSTEAPAPSALNSDVPASLDTVVAKAMRKDPNGRYQSADEMRADLVSVLRGQS
                     VEPAAVAAVAAGAVAAGDNATRVMTTQVPPATVPPDEVYRGVEEEPSSQMPFIITAFG
                     LLIALGILLFVLFRLAGGGDAEGELIEIPNVTGMTQEEARDRLEQDGFKVIFTPEASE
                     EIEVNLAIRSNPPAGTEAPEGSEVEVFMSTGIEEFAVPPLVGQNLEAAEAIIEQNGFV
                     LGEIDEQPNADFAEGTVISQDPGAGVLAGRGAAINLVISSG"
BASE COUNT          278 a          513 c          501 g          250 t
ORIGIN      
        1 tcatcgaggt ctggttgggg ggatccccat aggttgggcc aggaccgttc ctacaatcca
       61 acaacttcca tggaatccca gacagctctt accgatcgtt atgtgatcag ctcccacctc
      121 gcccgcggcg gcatggccga cgtgtacgag ggaatggaca cgctgctcaa ccgccgggtg
      181 gcgatcaagg tcctccattc ccagtactca gccgacgagg cgttcgtcaa gcgcttccgg
      241 cgcgaagccc aggccgcggc caacctgagc caccccaaca tcgtcggcat ctacgactgg
      301 ggccaggccc agaacaccta cttcatcgtg atggagttgg tcgacggccg ctcgctgcgg
      361 gacgtgcttc gcgccgaggg ggcgctgctc gcccgccgag ccaccgagat caccgccgag
      421 gtggctgctg cgctgtcggt ggcacaccag gccggcttgg tccatcgcga cgtcaagccc
      481 gggaacatcc ttctcgccaa ggacggaacg gtgaaggtca ccgacttcgg gatcgccaga
      541 gcgtgggatg acagcgctga gttgaccaag accggcgcag tgatcggcac tgccacctac
      601 ttcagcccgg agcaggccca gggcgccggc gccgacgccc gaagtgatgt ctatgcgctc
      661 ggtgtggtcc tctatgagat gctcaccggg aggcccccgt tcaccgggga cagcccgatg
      721 tcggtggcgt tccaacatgt ctccaccgag gcccccgccc cttccgcgct caattccgat
      781 gtccctgcct cactggacac cgtagtcgcc aaggcgatgc ggaaagaccc caacgggaga
      841 taccagagtg ccgacgagat gcgggccgat ctggtgtcgg tgctcagggg acagagtgtg
      901 gaaccagcgg cagttgccgc ggtggcggcc ggggcagtcg cggccggtga caacgccacc
      961 cgggtgatga ccacccaggt tcccccggcg acggttcccc ccgacgaggt ctaccgcggg
     1021 gtcgaggagg agccctcctc ccagatgcca ttcatcatca ccgcgtttgg gcttctgatc
     1081 gctctcggca tcctcctttt cgtcctgttc cgtctcgccg gcggcggtga cgccgaggga
     1141 gagctgatcg agatccccaa cgtcaccggg atgactcagg aggaagcgag agaccgtttg
     1201 gagcaggatg gcttcaaagt catcttcacc ccggaggcca gcgaagagat tgaggtcaac
     1261 ctggcgatcc gatccaaccc cccggccggc accgaggccc cagaaggctc ggaggtcgag
     1321 gtgttcatgt ccaccgggat cgaggagttc gcggtcccgc ccctggtcgg ccagaacctg
     1381 gaggccgccg aggccatcat cgaacagaac ggcttcgtgc tcggtgagat cgacgagcaa
     1441 ccgaacgccg acttcgccga gggcactgtg atcagtcagg acccgggcgc cggagtcctc
     1501 gccgggcgcg gggcggcgat caacctggtg atctcgagcg gg
//
LOCUS       DATITW010000669         1656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_233143, whole genome shotgun sequence.
ACCESSION   DATITW010000669 DATITW010000000
VERSION     DATITW010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233143"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VLS86_09360"
     CDS             <1..580
                     /locus_tag="VLS86_09360"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HSO50743.1"
                     /translation="EISRVVPMIEALTSESHLVAIDTWKPEVAHAAVDAGAVIINDTG
                     GLRDPEMRAVVAQAGVAAIAVYVEGANPHDVGEIEIRPDKARHTAQEFAHLISELEGE
                     GIHSVILDPGIALNYRGDYEAYTGMQLEVIAGSEELHNLGKPVLIPVPRKRDFHRVIA
                     YIALALEHRADIIRVHDVAVACDLARLFDRLP"
     gene            610..1593
                     /locus_tag="VLS86_09365"
     CDS             610..1593
                     /locus_tag="VLS86_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50744.1"
                     /translation="MGVLLISAPSNAGPGERQRMLDAAAERFSEQGVGRAELVRIDVP
                     GRGAGEDGDGSLRTELEPLVPLLQSGSLFGERQGLEIIDAQSLQVAEAEVLADLLTTA
                     DLEAIEIVILVTGTTPKPVAKVVQDRGKSVSVGKIWERQASQWLDAEIRDRGLHIDKG
                     GKEALLQKFGTDTAAMGGALDQLHGAPGKLTKQVILDRFKNRPDEPMFHITDAISKGD
                     VGVALRRLSDFLVHGHPLVYLAAIESDLKRRSLAAAAPDEATFRSWTGSRPADRGASR
                     VWSQRGKVRESSLRKAQEALVRADRVIKSQPEEVNRGTLERLTVALGRWYG"
BASE COUNT          302 a          503 c          566 g          285 t
ORIGIN      
        1 ggagatttcg cgggtggttc caatgatcga ggcactgacc tcggagtcac atctggtggc
       61 catcgacacc tggaagcctg aggtggctca cgccgccgtc gatgccggtg cggtcatcat
      121 caacgacacc ggaggactgc gcgatccgga gatgagagct gtagtggccc aggccggagt
      181 ggccgcgata gcggtgtatg tcgaaggggc caatcctcac gacgtgggcg agatcgagat
      241 acgacccgac aaggcccggc acacggccca ggaatttgcg catctgatct cggagctgga
      301 gggggagggg atacacagcg tgatcctcga cccgggtata gccctcaact atcggggcga
      361 ctacgaggct tacaccggga tgcagctcga agtgatcgcc ggcagcgagg agctgcacaa
      421 cctcggcaaa ccggtgctga tcccggtccc gagaaagcgt gatttccatc gggtcattgc
      481 ctacatcgcc ctggccctcg agcatcgggc cgacatcatc cgggtccacg acgtcgccgt
      541 cgcctgcgac ctggcgagat tgttcgacag gttgccgtaa ctgtcccctg cctgactacg
      601 gtcatgggca tgggcgtgct cctcatctcg gcgccatcga atgccggtcc cggcgaacgt
      661 cagcggatgc tcgacgccgc ggccgagcga ttctcagagc agggcgtagg tagagcggaa
      721 ctggttcgca tcgacgttcc ggggcgaggc gccggggagg acggtgatgg gtcgctccga
      781 accgagctcg agcctctggt cccgttgctg cagtcggggt ccctgtttgg ggagagacag
      841 ggtctggaga tcatcgatgc ccagagtctc caggtggcag aagccgaagt gctggctgac
      901 ttgttgacaa ccgccgacct ggaggccatc gagatcgtga ttctcgtgac cggaaccacc
      961 cccaagcctg tggccaaggt ggtgcaggat cgtggcaaat cggtttcggt gggcaagatc
     1021 tgggagcgtc aggcgtcgca atggctcgac gccgagatcc gagaccgtgg cctccacatc
     1081 gacaagggtg ggaaggaagc actcctccag aagttcggga ctgatacggc ggcgatgggc
     1141 ggggccctcg accaacttca tggggcgcct ggcaagctca ccaagcaggt gatcctcgac
     1201 cggttcaaga accggcccga tgagcccatg ttccacatca ccgacgcgat ctcgaagggc
     1261 gacgtcgggg tggcgttgcg gcgactgtcc gacttcttgg tccacggtca ccccctggtc
     1321 tacctggcgg ccatcgagtc cgatctgaag cgccgctcgc tggcggctgc cgctcctgac
     1381 gaggcgacct ttcgcagttg gaccgggtcc cgacccgccg accggggtgc ctcccgggtc
     1441 tggtcgcaac ggggcaaggt ccgggaatcg tcgttgcgca aggcccagga ggctttggtg
     1501 cgcgccgatc gggtgatcaa gtcgcaacca gaagaagtga atcggggtac gctggagcgg
     1561 ttgacggtcg ctctgggtcg ttggtacggc taacaagccc ccctcgcccg acctgcggtc
     1621 gggcggtccc cccgcttcgc agggggacaa cacaac
//
LOCUS       DATITW010000670         1753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_473175, whole genome shotgun sequence.
ACCESSION   DATITW010000670 DATITW010000000
VERSION     DATITW010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473175"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VLS86_09370"
     CDS             <1..265
                     /locus_tag="VLS86_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HSO50745.1"
                     /translation="RDGTKVTRAGIALTGVGGSTISAEEAARILVGGPLSAEVIGEAA
                     ELAAQAAQPRTDHRGSASFKRQVVRVFTTRILTRLVQSEEEAA"
     gene            267..788
                     /locus_tag="VLS86_09375"
     CDS             267..788
                     /locus_tag="VLS86_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSO50746.1"
                     /translation="MSNLYEVLTPVADDDVPVQTVTIRVNGEEMSAVVEPRLLLAHLL
                     RQGFKLTGTHTGCDTTNCGACTVLFDGQPVKSCTMFAVQANGHDVMTVEGLAGPSELH
                     ALQEGFKEEHGLQCGFCTPGMMMTSKALLEENPNPTEDEIRWALSGNLCRCTGYQNIV
                     KAVMWAAAKLQDQ"
     gene            846..>1753
                     /locus_tag="VLS86_09380"
     CDS             846..>1753
                     /locus_tag="VLS86_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSO50747.1"
                     /translation="MGASRRRVEDNRFIRGKGNYVDDIVLPGMLHMEILRSPLAHARI
                     RSIDTSKAWAMPGVRLVLTGETMAARNLAWMPTLSYDTQAVLATDKVRFQGQEVACVV
                     ADDPYIAKDACEAIIVDYEPLPPIVNPMQSLAPGAPLIRDDKENQADNVIYDWEVGDR
                     SGTDRAFAEADLVSKIELHYPRSHPSPIECCGSIADFNRSTSKLTVYLTTQAPHIIRA
                     AVALVAELPEHMIRIIAPDIGGGFGNKVPVYPGYVCSILCSILLGKPVKWIEDKSGNL
                     ISTGFARDMYFDGELALRKDGKILGIR"
BASE COUNT          334 a          581 c          565 g          273 t
ORIGIN      
        1 ccgcgacggg accaaggtca ccagggcggg catcgccctc accggcgtcg gcgggtcgac
       61 gatctcggcc gaggaagccg cgcggattct cgtcggtggg ccgctgagcg ccgaggtgat
      121 cggcgaagcc gccgaactgg ccgcccaggc cgctcagccc cgcaccgacc accgtggcag
      181 cgcctcgttc aagcgacagg tggtgcgcgt cttcaccacc cggatcctga ccagactcgt
      241 tcaatcggaa gaggaagccg cctgacatga gcaacctgta cgaggtcctc accccggtcg
      301 ccgacgacga cgtcccggtg cagacggtca ccatccgggt caacggcgag gagatgtcgg
      361 cggtcgtcga gccccggctt ctccttgctc accttctccg tcaagggttc aagctcaccg
      421 ggactcacac cggttgtgac acgaccaact gcggggcgtg cacggtcctc ttcgacgggc
      481 agcccgtcaa gagctgcacc atgttcgccg tgcaggcgaa cggccacgac gttatgaccg
      541 tcgagggcct ggccggcccc agcgagcttc acgccctcca ggaggggttc aaggaggagc
      601 atgggctcca gtgtgggttc tgcaccccgg ggatgatgat gacgtccaag gcgctgctgg
      661 aagagaaccc gaacccaacc gaggacgaga tccgttgggc gctgagcggc aacctctgtc
      721 gatgcaccgg ctatcagaac atcgtcaagg cggtcatgtg ggccgctgcc aagctccagg
      781 atcaatgaga tgaagccagc ggaggtagac aagtgacggc gctcgacgag atcaagatcg
      841 gcgggatggg cgccagtcgg cgccgagtcg aggacaaccg cttcatcagg gggaagggca
      901 actacgtcga cgacatcgtc ctgcccggga tgttgcacat ggagatcctg cgcagtcccc
      961 tggcgcatgc ccggatcaga tcgatcgaca cctcgaaggc gtgggccatg cccggggtgc
     1021 gtctggtgct caccggggag acgatggctg cgcgcaatct ggcctggatg cccacactct
     1081 cctacgacac gcaggcggtg ctcgccacgg acaaggtgcg cttccagggc caggaggtcg
     1141 cgtgcgtggt cgccgacgac ccctatatcg ccaaggacgc ctgcgaggcg atcatcgtcg
     1201 actacgagcc actccccccg atcgtcaatc cgatgcagtc gctggcccca ggggcgccgc
     1261 tgatccggga cgacaaggag aaccaggccg acaacgtcat atacgactgg gaggttggcg
     1321 acaggagcgg caccgatcgg gcgttcgccg aggccgacct cgtgtccaag atcgagctcc
     1381 actacccccg ctcccacccc tcaccgatcg agtgttgcgg gtcgatagcc gacttcaacc
     1441 ggtcgacctc gaagctgacc gtctatctca ccactcaggc gccacacatc atccgggccg
     1501 cggtggcact ggtggccgag cttcccgagc acatgatcag gatcatcgcg ccggacatcg
     1561 gcggcgggtt cggcaacaaa gtcccggtat atccaggata cgtgtgctcg atcctctgct
     1621 cgatcctgct cggcaagccg gtcaagtgga tcgaggacaa gtccggcaac ctcatctcca
     1681 ccggcttcgc gcgggacatg tacttcgacg gcgagctggc gttgcgcaag gacggcaaga
     1741 tccttggcat ccg
//
LOCUS       DATITW010000671         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_353031, whole genome shotgun sequence.
ACCESSION   DATITW010000671 DATITW010000000
VERSION     DATITW010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353031"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VLS86_09385"
     CDS             complement(<1..513)
                     /locus_tag="VLS86_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50748.1"
                     /translation="MALTDERQHEVDAPSRRIGISSILEGMGILGAVGTVALGVAGTI
                     PEEHELEVGREVFGNIPDAVQVLFYLTTAGFVWLAFHLFARRATAWSLGKPDRRTGNW
                     GHRVKTLGSGLRMTTLLRDPRAGLIHAAMYYGFVVLFLGTVTLEIDHLLPGNLKFLEG
                     GVYQGYSAILD"
     gene            complement(569..1423)
                     /locus_tag="VLS86_09390"
     CDS             complement(569..1423)
                     /locus_tag="VLS86_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HSO50749.1"
                     /translation="MITGSRPGKALARKFVAGDTLEDAVVVARDLNAKGFMVSLDLLG
                     EEVTDRDAALAARDEYLEGLNRIGAEDLKANISVKLTQLALTMDEALTVDLVDSLAGR
                     AEEIGTTVTIDMEDSRYTDATVRIYEKAQRAHANLGLALQACMHSTPADLDRLMPLGG
                     HLRLCKGAYVEQAEVALTSKGDVDAAFAAQLRVLMAHESVRPAVATHDLDLVDLTRQL
                     ARGRREPFEFQMLYGVRTGMQQGLLSEGYALRVYLPFGSHWYPYLTRRLAERPSNAWF
                     FVRSVFGG"
     gene            complement(1477..2289)
                     /gene="proC"
                     /locus_tag="VLS86_09395"
     CDS             complement(1477..2289)
                     /gene="proC"
                     /locus_tag="VLS86_09395"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019666514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HSO50750.1"
                     /translation="MAVHPSVAILGVGAIGEALFTGLKRAGWAAGDLALCVRREDRAT
                     ELAKRSRSLITMDPIEAIENRDVVVVSVKPRDVPAILDQLKMRVGKTQMILSLAAGIT
                     TATFEAKLGAVAVVRAMPNTPALVGEGVTGIAKGAYANGDHIKRATEILGAVGTVSVL
                     DEALLDAVTAVSGTGPAYVFLMAEALTEAAVREGLPRDIAETFVHQTIRGAGHLLTDT
                     DSSPAELRSQVTSPGGTTAAAVHVLEERGFRALVEDAVRAAARRSAELGEQV"
BASE COUNT          478 a          870 c          820 g          435 t
ORIGIN      
        1 atcgaggatc gccgagtaac cctggtacac cccgccctcg aggaacttca ggttcccggg
       61 gaggagatgg tcgatctcga gggtcaccgt gcccaggaag aggaccacga agccgtagta
      121 catcgcggcg tggatgagac cggcccgcgg atcgcggagc aatgtcgtca tgcgaagacc
      181 ggagccgagg gtcttcaccc ggtggcccca gttccctgtg cggcgatccg gcttccccag
      241 cgaccacgcg gtcgcccgac gtgcgaagag atggaatgcc aaccagacga agcccgcagt
      301 cgtcaggtag aagaggacct ggaccgcgtc ggggatgttg ccgaagacct cccgacccac
      361 ctccagttcg tgctcctccg ggatggtccc ggcgacaccc agggccaccg tgcccacggc
      421 acccaggatg cccatgcctt ccaggatcga ggaaatgcct atccgtcggc tcggggcgtc
      481 gacctcatgc tgccgctcgt cggtcagggc catgaccggc gagtgtagga gggccgattt
      541 ccggtgaccg catcatcaga agccgacatc aacccccgaa cacggagcga acgaaaaacc
      601 aggcgttgga tggccgctcg gcgagacggc gcgtcagata tgggtaccag tgggagccaa
      661 acgggagata gaccctgagt gcgtaaccct cggacaacag cccctgctgc atgccggtgc
      721 gaaccccgta cagcatctgg aactcgaatg gctcacggcg accgcgggcc agttggcggg
      781 tcagatccac gagatcgagg tcgtgagtgg ccacagccgg tctcaccgac tcgtgagcca
      841 tcaacaccct gagctgtgcg gcgaaagccg catcgacatc gcccttcgac gtgagcgcca
      901 cctcggcctg ctccacgtac gcccccttgc acaatcgaag gtgaccacca agtggcatca
      961 aacggtccag atcggccggg gtgctgtgca tgcacgcctg gagcgcaagc cccaggttgg
     1021 catgagccct ctgcgccttc tcgtagatac ggaccgtggc atcggtgtag cgggaatcct
     1081 ccatgtcgat ggtgactgtg gtcccgatct cctcggctcg acccgccagg ctgtcgacca
     1141 gatcgaccgt gagcgcctca tccatggtca aagcgagttg ggtgagtttg accgaaatgt
     1201 tggctttcag gtcctcggcg ccgatccggt tcagaccctc caggtactcg tcccgggccg
     1261 cgagcgcagc gtcacggtcc gtcacctcct cccccagcag gtcgagggag accatgaacc
     1321 ccttggcgtt gaggtcgcgg gccaccacga ccgcgtcttc gagggtgtcg ccggccacga
     1381 acttccgggc cagcgccttg ccgggtcggc tcccggtgat cattctctcc acaggccgag
     1441 cctgagtggt cgtgacgacc accctggaga aaacactcaa acttgctccc cgagctccgc
     1501 cgacctcctg gcagcagccc gaaccgcgtc ttccaccagt gcccgaaacc cccgctcctc
     1561 gagcacatgg accgcagccg cggtcgtgcc acccggggat gtcacctggg atcggagctc
     1621 ggcagggctg gagtcggtgt cggtgaggag gtgccctgcc ccccggatcg tctgatggac
     1681 gaaggtctcg gcgatgtcac gcggcaagcc ctcgcgtacc gcagcctcgg tgagagcctc
     1741 ggccatgagg aacacgtaag ccgggccggt tcccgacacc gcggtgaccg cgtcgagcaa
     1801 ggcctcgtcg aggacgctga cggtgcccac cgcccccagg atctcggtag ctcgcttgat
     1861 gtggtcgccg ttggcataag cgcccttggc gatgccggtg acgccctccc cgaccagggc
     1921 cggggtgttg ggcatcgccc ggaccactgc caccgctccc agcttggcct caaaggtcgc
     1981 ggtggttatc ccggccgcca aagagaggat catctgagtc ttgcccaccc gcatcttgag
     2041 ctggtcgagg atggcgggga catcccgcgg cttcaccgag accactacga cgtcccgatt
     2101 ctctatcgcc tcgatcgggt ccatcgtgat cagtgatctc gatcgctttg ccagttcggt
     2161 cgcccggtcc tcacgtcgta cgcagagtgc gaggtccccg gcggcccagc cggcacgctt
     2221 caagccagtg aacagagctt cgcctatggc cccgacgcca aggatcgcaa cggaaggatg
     2281 aacagccata tcccgattat atggagatat cagattgggg aaccgatgaa tccgagatca
     2341 gatctgatca ggtcgagcac ggtcaggtct cgctgcacgc agacaggtcg gcaatgatca
     2401 gcgggcttgg atcggccgtc atgaccggat gggcatcaga tgcccgggcg agctacttct
     2461 cgccgactct gcttcggaga cggcgaatct cgtcgtagac accatcggtg agggtggtgt
     2521 tggcccgggc agagacgccc ttcatgcccg ccagcacccg gtccggcctt ggggtcggca
     2581 ccaccggctc ggtctgctgc tca
//
LOCUS       DATITW010000672         2698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_754117, whole genome shotgun sequence.
ACCESSION   DATITW010000672 DATITW010000000
VERSION     DATITW010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754117"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VLS86_09400"
     CDS             <1..562
                     /locus_tag="VLS86_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012998528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSO50751.1"
                     /translation="QMARFHLLCAGYGGGRVASTVSLVESGSSLVVIDPGMVSSVSVI
                     LDPIRALGRSPEEVTDVILSHHHPDHTINVALFPKARVHDHWAIYKDDVWTARPAEGF
                     EVAEDVTLWETPGHTAQDITTIVRQDDQTLAFSHLWWYEDAPFHDPLCTDPDGLHVGR
                     ERVLEVATLIIPGHGEPFTPSESTSR"
     gene            572..1747
                     /locus_tag="VLS86_09405"
     CDS             572..1747
                     /locus_tag="VLS86_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSO50752.1"
                     /translation="MAAPGPVEELASKDAYLAEAEGRVLEVVKGGILLDRTVFYARGG
                     GQPGDIGVLRWDGGEVEVLDTIRHEGKPLHVVDAEAALPGPGTSVYGVIDWERRYRTM
                     KTHTALHALSGVVFRDFGAKVTGGNMEPGMARMDFELDGISVEFGQQVERKLNEELTK
                     GYPTEILYLARQEALQDPDLIRTKVNLIPEWVEEIRVVDIVGLDRQADGGTHVASTLE
                     VGHVTVVKTESKGKANKRMRIELTGQSRPLPSVHQSSERHPMSVIVGYLSSDRGIKAL
                     ERGIEEAKLRNTNLVVVHSLHGVGKDDDEDVIKSDRDIAALEESLQGKGVEFSVHNYV
                     RGNEPAQDLIRAAEEFDGELIVIGLRQRSSAGKFLLGSNAHDILMDAPCPVLTVKAD"
     gene            1842..>2698
                     /locus_tag="VLS86_09410"
     CDS             1842..>2698
                     /locus_tag="VLS86_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HSO50753.1"
                     /translation="MIDFILGLFLAGLLVRGWLRGFVRESLDLVGLVVGLWVAFRLSG
                     PLGEFLTDRFGVSPEVATIGGGITLFLLFGIAMSIAAHYLSKVMNLPGLTLINRFGGA
                     AVAAAWGVAIVLVIVNLQRAMPLPPSLDQRFEDSTVAQAIAGPEALPQQVFESLGSDG
                     VLSSLASMRSLFGANRAVPEGDEVLTIPPAGPDEVRQAREEAVEVLSQVNEHRTEQGL
                     SALGVSGSLNRVAETRAVEMYTTGRISRDHPPGGNVADDLAEAGIRLARMGENLALAS
                     SSRAAVDGMT"
BASE COUNT          474 a          838 c          917 g          469 t
ORIGIN      
        1 ccagatggcc agattccatc tgctctgcgc cggatacggc gggggaaggg tcgcatcgac
       61 ggtgtcgctg gtggagtcgg ggtcttcgct ggtggtcatc gacccgggga tggtctcgtc
      121 ggtttcggtc atcctcgacc cgatccgggc gctggggaga tctcccgagg aggtcaccga
      181 cgtgatcctg tcccatcatc accccgacca caccatcaac gttgcgctct tccccaaggc
      241 ccgggttcac gaccactggg ccatctacaa ggacgacgtg tggacggcgc gccccgcgga
      301 gggattcgag gtggccgagg acgtcactct ctgggagacc ccgggccaca ccgcccagga
      361 catcacgacc atcgtccgcc aggacgatca gaccctggct ttcagtcacc tgtggtggta
      421 cgaagacgca ccgtttcacg acccgctgtg caccgacccc gacggtctcc atgtgggcag
      481 ggagcgggtg ctcgaggtcg ccaccctcat catccccggc catggcgagc cgttcactcc
      541 ttcagaatcg acctcccggt agcctcggag catggccgca ccaggacccg tcgaggagct
      601 cgcctctaaa gacgcctatc tcgccgaggc ggagggcagg gtgctggaag tggtcaaagg
      661 ggggatcctg ctcgaccgga cggtgttcta cgcccgaggc ggcggtcaac ccggtgacat
      721 tggagtgctg cgctgggatg gaggcgaggt cgaagtcctc gacaccatcc gtcatgaagg
      781 caagcccttg cacgtggtgg atgcagaggc ggccctcccc ggcccgggca cttcggtgta
      841 cggcgtgatc gactgggagc gtcggtatcg gaccatgaag acccacaccg cccttcacgc
      901 attgtccgga gtcgtctttc gagacttcgg agccaaggtc accgggggca acatggagcc
      961 ggggatggcc cggatggact tcgagctcga cgggatctcg gtcgagttcg gccagcaggt
     1021 ggagcgaaag ctgaacgagg agctgaccaa gggctacccg accgagatcc tctatctggc
     1081 ccgccaggaa gcgttgcagg acccggacct gatccgaacc aaagtgaacc tgatcccgga
     1141 gtgggtggag gagattcggg tggtggacat cgtcggtctc gatcgccagg cggatggtgg
     1201 cacccacgtg gcatccacgc tcgaggtggg ccacgtcacc gtggtgaaga ccgagtcgaa
     1261 gggcaaggcc aacaagagga tgaggatcga gctcaccggc caatcgcgtc cgctaccttc
     1321 cgttcaccag tccagcgaaa ggcaccccat gagcgtgatc gtcggatacc tgtcctcgga
     1381 ccggggaatc aaagccctgg agaggggtat cgaggaggcc aagctccgaa acaccaattt
     1441 ggtcgtggtc cactcgctgc atggtgtcgg gaaggacgat gacgaggatg tcatcaagag
     1501 cgatcgtgac atcgctgccc tcgaagagtc gcttcagggc aagggagtgg aattctcggt
     1561 ccacaactat gtccgcggca acgagccggc gcaggacctc atccgggccg ccgaggagtt
     1621 cgacggtgag ctgatcgtca tcggtcttcg tcagcgcagc tcggcgggga agttcctgct
     1681 ggggtcgaat gcacacgaca tcctcatgga tgccccatgt cctgtcctca ccgtgaaggc
     1741 ggactgagca gaggcgggcc tccggcttcg caacgcccct gaaccggcat cggctcgata
     1801 tgtcggttca ggcaccaggc agctagtacc aagtacccga gatgatcgac ttcatcctcg
     1861 gtttgtttct cgccgggctg ctggtccggg gttggctgcg gggctttgtc cgggagtcac
     1921 tggacctcgt cggcctggtg gtggggctct gggtcgcctt ccggctgagc gggccgttgg
     1981 gcgagttcct caccgatcgc ttcggcgtgt ccccggaggt ggccacgatt ggcggcggga
     2041 tcaccctgtt tctcctgttt gggatcgcga tgagcatcgc cgcccactac ctatccaagg
     2101 tgatgaatct gcccggattg accctgatca acaggttcgg tggggccgcg gtggccgcgg
     2161 cgtggggagt ggccatcgtc ctcgtgatcg tcaacctgca gcgggccatg cctctccccc
     2221 cgagcctcga tcagcgattc gaggattcga ctgtcgccca ggccatcgcc ggcccggagg
     2281 ccctgcccca acaggtcttc gaaagccttg gctctgacgg tgtgctcagc tcgctggcgt
     2341 cgatgcggag tctgtttggc gccaaccgcg cggtcccgga aggcgacgag gtgctgacca
     2401 tccctccggc cgggccggac gaggtgcgac aggcgaggga ggaggcggtc gaggtcctca
     2461 gccaggtcaa cgagcaccgg accgagcagg gtctgtcggc attgggtgtc tccggcagtc
     2521 tcaaccgggt ggccgagacc agggcggtcg agatgtacac cacgggacgc atctcgcgag
     2581 accacccacc cggggggaac gtggcagacg acctggccga ggccgggatc cgtctggcca
     2641 ggatgggtga gaacctggcc ctggccagca gctcgcgtgc cgcggtggac ggcatgac
//
LOCUS       DATITW010000673         1553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_33434, whole genome shotgun sequence.
ACCESSION   DATITW010000673 DATITW010000000
VERSION     DATITW010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33434"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VLS86_09415"
     CDS             complement(<1..454)
                     /locus_tag="VLS86_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50754.1"
                     /translation="MSLVHAEYLKLSRRKLYPVMVLILAFLVALTAFFLLIFGQIAPE
                     LAEDVPVLTKPTADLIGAQQVAGQTWFPLILAVVLIGADLSSTVWATSLTRDPSKPAH
                     IGARILVVGAASWLAMLLGTAGWAVVVAIASPGEGGPSISEWLDVFWKI"
     gene            complement(451..1353)
                     /locus_tag="VLS86_09420"
     CDS             complement(451..1353)
                     /locus_tag="VLS86_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50755.1"
                     /translation="MVGSSTAITVEGLIKHYKKSKALDGLDFTIPSGAITGFLGPNGA
                     GKTTTFRSLLGLTRPQAGRIEVLGHQVPGDLKVLTKQVGAIIEEPGLIRALGGRINLQ
                     VAADTLGFGHERIDELLEFVELTYDAKRRVEEYSKGMRQRLALAAALLGEPELLLLDE
                     PLDGLDPAGQRGFRERLRQLADAGRTVVVSSHDLADIEALADYVVVINHGRLVAQGYL
                     EELLGGAGGTRVIVADQAAAVAALAAAGIEATAEASSLTVHEGDGSRIVEVLARSGIY
                     PSEVMSARSSLESVVLGMTTEATE"
BASE COUNT          275 a          518 c          509 g          251 t
ORIGIN      
        1 cgatcttcca gaagacgtcc aaccactccg agatcgaagg cccgccctcg ccgggcgagg
       61 cgatggccac cactacggcc cagccggcgg taccgagcaa catcgccagc catgaggctg
      121 ccccgaccac caggatccgc gccccgatgt gagccggctt gctcgggtcg cgagttagtg
      181 aggtcgccca gaccgtcgag ctcaggtcgg cgccgatcaa taccaccgcg aggatcagcg
      241 ggaaccaggt ttggcccgcc acctgttgtg cgccgatcag gtcggcggtc ggcttggtca
      301 gcaccggaac gtcctcagcc agttcgggag cgatctgtcc gaagatgagg aggaagaacg
      361 cagtcagcgc gaccaggaaa gcaaggatga ggaccatcac cgggtacaac ttgcgcctgc
      421 tcagcttgag gtattcggcg tgaaccaggc tcattccgtc gcctcggttg tcatccccag
      481 gaccaccgat tccagcgagc ttcgcgcgga catcacctcg gatggataga tgccggatcg
      541 ggccagcact tcgacaatcc ggctgccgtc gccttcatgc acggtcaggg aactcgcctc
      601 ggcggtggct tcgatcccgg ccgcggcgag ggcggcgacc gccgctgcct ggtcggccac
      661 gatcacccgg gtgccccctg cgcccccgag cagctcttcg agatagccct gggccaccag
      721 acggccgtgg ttgatgacga cgacgtagtc ggccagggcc tcgatgtcgg cgagatcgtg
      781 actggacacg accacggtgc gccccgcatc ggcaagctgg cggagccgct cgcggaaacc
      841 tctctggcct gcggggtcga ggccgtcgag cggctcgtcg agcaggagaa gctcaggctc
      901 gccgaggagt gccgcggcca gagcgagacg ctggcgcatc ccctttgagt actcctcgac
      961 gcgccgtttg gcgtcgtagg tgagctcgac gaattccagt agctcgtcga tgcgctcgtg
     1021 accgaacccg agggtgtcgg ccgcgacctg gaggttgatc cttcctccca gtgccctgat
     1081 cagccccggc tcttcgatga tcgcaccgac ctgtttggtc aacaccttca gatctcccgg
     1141 cacctgatgg ccgaggacct cgatacggcc ggcctggggc cgggtgagac cgagcagcga
     1201 ccgaaacgtg gtggtcttgc cggctccatt gggaccgaga aacccggtga tggcacccga
     1261 cgggatggtg aagtcgaggc cgtcgagggc cttcgacttc ttgtaatgct tgatcagccc
     1321 ttcgactgtt atcgcggtgg aggagccgac cattgcgggg aagctaggga cactccgaaa
     1381 ctcgggtcaa accccgccgg acacccaatg gaaggtgacg gcgtcgggag cgacccgacc
     1441 cccgacgaag tcgaccctgt tggcgcccga caggcgagcg aggcgaccaa ccgtctcccg
     1501 cttggcaggg tcggccgcgt cgattggctc tccctcaccg gtgatcgtcc gca
//
LOCUS       DATITW010000674         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_233624, whole genome shotgun sequence.
ACCESSION   DATITW010000674 DATITW010000000
VERSION     DATITW010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233624"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1104)
                     /locus_tag="VLS86_09425"
     CDS             complement(97..1104)
                     /locus_tag="VLS86_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HSO50756.1"
                     /translation="MSVDLSTRYLGLDLAHPVLPSASPLTGDIDHIHALVGAGASAIV
                     LPSLFEEQIEHNAMAVHQSLELGADGFGEAPDGFFPQLDDYNTGPKDYLTLVRQAKKE
                     IDVPVIASLNGTSTGGWTLYARILADEGIDALELNTYQIAASIKHGASEIEGGYLRLV
                     ETIRGAIEIPLAVKIGPFFSSTANMAGRLVDAGADALVIFNRLYQPDIDTQTLEVIPK
                     LALSEPEELRLVLRWLAILHGRIECDLAATTGVHEADDAVKAILAGATVTMMASALLR
                     HGPIRLTETKNGLETWLAEREYASVDQARGSMSYQSVPDPSAFERAGYMKTLTSYVPS
                     W"
     gene            complement(1101..>3049)
                     /locus_tag="VLS86_09430"
     CDS             complement(1101..>3049)
                     /locus_tag="VLS86_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSO50757.1"
                     /translation="QLWVIDAAQIAHDAGLGGRVNTVLQTCFFHLTGLLPADDAVSSI
                     KEHIRKTYGNRGQAVVKRNEDAVDRAVAGLLRVQVGPVADGRAMADPVPTVAPDFVTR
                     VTAAMMAGRGDLLPVSALPVDGTFPTGTARWEKRSIAEEIPIWDPEICIDCGRCALVC
                     PHAAIRFKAFDAGTTIPDGFLWKEPTGTDLAGQRIAIQVAPEDCTGCGICVEVCPAKS
                     KDVIKHKALNMAPKHDHLDRERRNFEVFLDIPETDRALVNAKSVKGSQLLEPLFEFSG
                     ACAGCGETPYLKLLTQMFGDRMIVANATGCSSIYGGNLPTTPWTVDGNGRGPAWSNSL
                     FEDNAEFGLGMRLAMEAQRSDAVLLVTALAPELGQLAADILELVEDPDHSEQDALIAR
                     QRELVAELKVRLSDMEGRLPRRLEGVADSLIETSIWIVGGDGWAYDIGFGGLDHVLGS
                     GRNLNILVLDTEVYSNTGGQASKATPRAAIAKFAAGGKKTGKKDLGQIAMAYGDVYVG
                     QIAMGANPTQTVKTIAEAEEHRGVSLVIAYSPCIAHGLDMRYQLSHQKAATDSGYWPL
                     YRYDPARESKGEPGLRLDSRAPTIRFEEFARSEARFAMLGKVDPEGEEELLRRAQADI
                     NDRWHYYEQMVHVHRTAEYAEVEE"
BASE COUNT          527 a          946 c          968 g          608 t
ORIGIN      
        1 gccggcgatc tcgatgccct ggcggccgag gtcgaggcag ccgactccgg tctcgaagag
       61 tcgtgactgg agacgttcgt ggacggcagc ccttggtcac caagagggca cgtaagacgt
      121 gagtgtcttc atgtacccag ctcgttcgaa agcagatgga tcgggaacgg actgatagct
      181 catgctgccc cgagcctggt cgaccgacgc atactctcgc tcggcaagcc aggtttcgag
      241 accgttcttc gtctcggtca acctgatggg accgtgacgt agcaaggcag aggccatcat
      301 tgtgactgtg gcgccggcga ggattgcttt gactgcatca tcggcctcat gaacaccggt
      361 agtcgccgcg agatcgcact cgatccggcc gtggaggatg gccagccagc gcaacacgag
      421 tctcagttcc tccggctccg atagcgccag cttgggaatc acctcgagag tctgtgtgtc
      481 gatgtccggt tggtacagcc ggttgaagat gacgagggcg tcggctccgg cgtcgaccag
      541 tcgacccgcc atgtttgcgg tcgaggagaa gaaggggccg atcttcacgg cgagtgggat
      601 ctcgatcgcg ccgcgtatgg tctcgacgag gcgtaggtag ccgccctcga tttcgcttgc
      661 cccgtgcttg atgctggcgg cgatttggta ggtgttgagc tccagagcgt cgatgccctc
      721 gtcggcgagg atccgggcat agagagtcca accccccgtc gaggtgccgt tgaggcttgc
      781 gatcaccggc acgtcgatct ccttctttgc ctggcggacc agtgtcaggt agtccttcgg
      841 tccggtgttg tagtcgtcga gctgtggaaa aaacccgtcg ggcgcctcgc caaatccgtc
      901 ggcgcccagc tcgaggctct gatgtacagc catcgcgttg tgttcgatct gctcctcgaa
      961 gagactcggt aggacgatgg cagacgcgcc ggccccgacc agggcgtgga tgtggtcgat
     1021 gtcacccgtc agcggtgacg ccgacggcaa gaccgggtga gcgagatcga gtcccaggta
     1081 tctggtcgac aggtcgacac tcattcttct acctccgcat actcggcggt ccgatgaacg
     1141 tgcaccatct gctcgtagta gtgccaccgg tcattgatat ccgcctgtgc ccgtcggagc
     1201 agctcctcct caccttcggg atcgaccttc cccaacatgg cgaatcgagc ttcagagcgg
     1261 gcgaactcct cgaagcggat ggtcggggca cggctgtcga ggcgtaagcc gggctcgccc
     1321 ttggattctc gggccgggtc ataccgatag aggggccagt aacccgagtc ggtggcggct
     1381 ttctgatgac tcaactggta cctcatgtcg aggccatgag cgatgcatgg gctgtaggcg
     1441 atgaccaggg agactcctct gtgttcctcc gcctcggcga tcgtcttgac ggtctgggtc
     1501 gggttggctc ccatggcgat ctggcccaca tacacgtcgc cgtaagccat cgctatctga
     1561 cccaggtcct tcttgccggt cttcttgccc ccggccgcga acttggcaat cgcggctcgg
     1621 ggtgtggctt tggaggcttg tcctccagtg ttcgaataga cctcggtatc gagcacgagg
     1681 atgttgaggt tgcgccccga ccccagaacg tggtcgagac ctccgaagcc gatgtcgtat
     1741 gcccagccgt cgccgccgac tatccagata ctggtctcga tcagcgagtc ggcgacacct
     1801 tcgagccgcc tcggcagccg gccctccatg tcggagaggc gcaccttcag ctcggccacc
     1861 agctcacgct ggcgggcgat gagcgcgtct tgctcggagt ggtcagggtc ctccaccaat
     1921 tccaggatgt cggccgccag ttggccgagc tcgggggcga gggcggtgac gaggagaacg
     1981 gcgtcgctgc gttgggcttc catcgcgagc ctcatgccca acccgaactc ggcgttgtcc
     2041 tcgaacaggc tgttggacca ggccggcccc cggccattgc cgtccacagt ccagggagtc
     2101 gttggaagat ttccaccata gatcgaggaa caaccggtgg cgttggccac gatcatgcgg
     2161 tccccgaaca tctgtgtcag gagcttgagg tagggcgttt ccccgcatcc ggcacaggca
     2221 ccggagaact cgaacaatgg ctccagcaac tgtgatcctt tcaccgactt ggcgttgacc
     2281 agcgcacggt cggtctcagg tatgtcgagg aagacttcga aattccgtcg ctcccgatcg
     2341 aggtggtcgt gcttcggcgc catgttcaat gccttgtgct tgatcacgtc cttcgacttg
     2401 gccgggcaca cctcgacgca gataccacat ccggtgcagt cttccggcgc cacctggata
     2461 gcgatccgct ggcctgccag atccgttccg gtcggctcct tccacaagaa cccgtcgggg
     2521 atggtggtgc cggcgtcgaa ggccttgaag cggatggcgg catggggaca gaccagcgca
     2581 caacggccac agtcgatgca gatctcgggg tcccagatcg ggatctcctc ggcaatagat
     2641 ctcttttccc accttgctgt gccggtcgga aaggtcccat ccaccggaag cgccgacacg
     2701 gggagcaggt ctcctctgcc cgccatcatc gctgcggtta cccgcgttac gaagtcggga
     2761 gcgacagtcg ggacagggtc cgccatcgcc ctgccgtcgg cgactggccc cacctggacc
     2821 cgaagtagcc cggcaacggc ccggtcgaca gcgtcctcgt tgcgtttgac cacggcctgc
     2881 cccctgttgc cataggtctt tcgaatgtgc tccttgatcg agctgacggc gtcatccgca
     2941 ggtagaagac cggtgagatg gaagaagcag gtctgaagga ccgtgttgac ccttcctccc
     3001 aggccggcgt catgggcgat ctgagccgcg tcgatgaccc agagctgta
//
LOCUS       DATITW010000675         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_73672, whole genome shotgun sequence.
ACCESSION   DATITW010000675 DATITW010000000
VERSION     DATITW010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73672"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1018)
                     /locus_tag="VLS86_09435"
     CDS             complement(116..1018)
                     /locus_tag="VLS86_09435"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HSO50758.1"
                     /translation="MAHSEKLELVEAIDTEVERLTAEHLARRSDWYFHDIVPWERGES
                     FRDKPWDVSQCTISENARTALVLNLLTEDNLPYYHSLIERYMPQTSAFSTWNHLWTAE
                     EGQHAIAIRSYLLTSRNCDPYELEDDRKATMINGYHPSYDDPTEIFAYTAAQELATRV
                     SHRNAGKITDDPAAFELMRNIATDENHHFIFYKSVMQAMLDQAPDQVLGGIYRVFDNF
                     QMPGVAMPNFLRRSVEVAKAGVYNLRIHHDRVVLPLIKDWKIAELAGLKPAAAELQDR
                     ILALPAQIMEKAERFEKRVGMSYA"
     gene            1147..2016
                     /locus_tag="VLS86_09440"
     CDS             1147..2016
                     /locus_tag="VLS86_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO50759.1"
                     /translation="MGTPASRRHLRTGVSILALIGVLVLVWESYKLIWTALGWEWPVR
                     PDDRTLPHTWDILLTLFQPAQRNGPLFIFVESRAALLTLREAFVGFVVGAGIGFGLGV
                     LFVRSRTAERAFMPYVVASQTVPILAIAPMVVVWGGRLDVPQWLTVSIIAAYLTFFPV
                     AINTLRGLRSTPSTALELMRSYASNPRETLWKLQVPTAMPYIFTALKVSAAASVIGAI
                     VGELPAGFRGGLGRDLLQYSQQFTAAPEKLFAAVLIAGLAGMAFVGLVTLAEHRWVGR
                     GRRRSTELQGTVG"
     gene            2013..>2666
                     /locus_tag="VLS86_09445"
     CDS             2013..>2666
                     /locus_tag="VLS86_09445"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50760.1"
                     /translation="MSLVVEAKDIGKVFGSGPDRTEALRGIDLVIAQGDFVSLIGPSG
                     CGKSTLLRLIGDLVGPTTGTISINGKTPGQAREDRDYGIVFQSATLLDWRTVQKNVEL
                     PLEIMDFPAGERPARAREMLDLVQLGGFADHFPWQLSGGMQQRVAIARALAFKPSLLL
                     MDEPFGALDEMTRELMQQEVLRLWEETGTSVVFVTHSIPEAVFLSTRVVVMSPRPGRI
                     "
BASE COUNT          417 a          815 c          899 g          535 t
ORIGIN      
        1 ttcttcttcc ccacggccac caggctcgat cgcaccaggt cggtcgacgt gccgaggaca
       61 gggctggccg accccaccga cgagcctcgc tcagtcgggc actgagcggt cggggtcagg
      121 cgtaggacat cccgacccgc ttctcgaaac gctcggcctt ctccatgatc tgggcgggca
      181 gggccaggat cctgtcctgc agctcggcgg cagccggctt gagcccggcg agctctgcga
      241 tcttccaatc cttgatcagc ggtagaacca ctcgatcgtg atggatccgg aggttgtaca
      301 cgccggcctt ggcgacttcg accgatcgtc gcaggaagtt gggcatggcc actcccggca
      361 tctggaagtt gtcgaacacg cggtagatcc cgcccaggac ctgatccgga gcctgatcca
      421 acatcgcctg catcacgctc ttgtagaaga tgaaatggtg gttctcgtca gtggcgatgt
      481 tgcgcatgag ctcgaaggcc gccgggtcgt cggtgatctt gcccgcgttg cgatggctca
      541 ctctggtggc cagctcctgg gccgcggtgt aggcgaagat ctcggtcgga tcgtcgtatg
      601 aaggatggta tccgttgatc atcgtcgcct tgcgatcgtc ttccagctcg tatgggtcgc
      661 agttgcgcga ggtgagcagg tagctgcgga tcgcgatcgc gtgctggccc tcttcggcgg
      721 tccacagatg gttccacgtc gagaatgccg aagtctgtgg catgtacctc tcgatcaggg
      781 agtggtagta ggggagattg tcctcggtga ggaggttgag caccagggcg gtcctcgcgt
      841 tttccgagat ggtgcattgg gagacatccc atggcttgtc ccggaagctc tcgccccgct
      901 cccaggggac gatgtcgtgg aaataccagt cagagcgacg tgcgagatgc tcggcggtca
      961 gtcgttcgac ctcggtgtcg atagcctcga cgagttcgag cttctcggag tgtgccatgg
     1021 ggatccttgg gtggagcgga ggggggctga cggtagttag cgagtacgtg gcctcataac
     1081 tgtcgccgat tggacccagg ggaggccggt cgggttacac cgatggtcgt tagagtctcc
     1141 gcctccatgg gcacaccagc cagtcgtcga cacctccgca ccggcgtgtc gatcctggct
     1201 ctgatcggtg tcctcgtcct ggtttgggag agctacaagc tgatttggac cgcgctcggc
     1261 tgggagtggc cggtccgacc cgacgatcga acgctgcccc acacctggga catcttgctc
     1321 accctgttcc agccggcgca gcgcaacggg cccctgttca tcttcgtcga gtccagggcc
     1381 gccctgctca ccctgcggga ggcgttcgtc ggtttcgtcg tcggggcggg catcgggttt
     1441 gggctggggg tgttgttcgt gaggtcccgt accgccgagc gcgccttcat gccctacgtc
     1501 gtcgcctccc agaccgtccc catcctggcc atcgccccga tggtcgtggt gtggggtggg
     1561 cgacttgatg tcccgcagtg gctgacggtc tcgatcatcg ccgcctatct gaccttcttc
     1621 ccggtggcga tcaacacgct gcgcgggctt cgatcgaccc cttccacggc cctcgagctg
     1681 atgaggagct atgccagcaa cccacgggag acactgtgga agctccaagt gccgacggcg
     1741 atgccgtaca tcttcaccgc gctcaaggtc tccgcggcgg caagcgtgat cggggcgatc
     1801 gtcggcgagc tgccggctgg gttccgcggc ggtctgggtc gggacctcct ccagtactcc
     1861 cagcagttca ccgcagcacc ggagaagctg tttgccgcgg tgctgatcgc gggtctggcc
     1921 ggcatggcct tcgtcggact ggtgacgctg gccgagcacc gctgggtggg aaggggtcgg
     1981 agacgatcca ccgagttgca ggggactgtt ggatgagcct ggtggtggag gccaaggaca
     2041 tcggcaaggt cttcggctcc ggcccggacc ggaccgaggc gctccgtggg atagacctgg
     2101 tgatcgccca gggcgacttc gtctcattga tcggcccttc cggttgcggc aagtcgacgc
     2161 tgctccggct gatcggcgat ctggtcggcc cgacgacagg cacgatctcc atcaacggga
     2221 agactcccgg tcaggcgaga gaggaccgcg attacgggat cgtcttccag tcggcgacat
     2281 tgctcgactg gcggaccgtg cagaagaacg tcgagctgcc actcgagatc atggactttc
     2341 cggcagggga gcgaccggca agggccaggg agatgctgga tctggtccag ctcggcgggt
     2401 tcgccgacca tttcccgtgg cagctctccg gtgggatgca gcagcgagtg gcgatagcga
     2461 gggccctcgc cttcaagcct tcgctcctcc tcatggacga gcctttcggc gccctcgacg
     2521 agatgacgag ggagttgatg caacaggagg tacttcgact gtgggaggag accgggacca
     2581 gcgtcgtgtt cgtcacccat tccattcccg aagcggtgtt tctctccacc agggtcgtcg
     2641 tgatgtctcc ccgccccggg cggatc
//
LOCUS       DATITW010000676         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_233779, whole genome shotgun sequence.
ACCESSION   DATITW010000676 DATITW010000000
VERSION     DATITW010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233779"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VLS86_09450"
     CDS             complement(<1..227)
                     /locus_tag="VLS86_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50761.1"
                     /translation="MAKFKATLRLPGDNQALPATVHIDDGRMQVASGEHVIGDWDIKT
                     IEISRIPEGFRVAAEGEVLLLDIEDRASFEE"
     gene            317..1237
                     /gene="coaA"
                     /locus_tag="VLS86_09455"
     CDS             317..1237
                     /gene="coaA"
                     /locus_tag="VLS86_09455"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HSO50762.1"
                     /translation="MPLSHPSYLDFTSDEWAALRANTPLTLTESDVDKLRGINVNLDI
                     DMVEHSFLPLSRLLNLYVLGSHQLATVTDTFLGAPPQPRPFVLGVAGSVAVGKSTISR
                     VLQELLARWPQHPQVELVTTDGFLYPNHVLEERGLMERKGFPESYDRRALLEFVARSK
                     SGSEHLEVPVYSHFAYDVVPGETRVISRPDILILEGLNVLQAGEKGAFVSDYFDFSIF
                     VDASPEAVREWYIQRFITLRDTAFAQPDAYFHRYANLSDEEAVAVATDIWTRINEVNL
                     FENILPTRERATLILEKAPDHSIERVRLRR"
     gene            1268..1780
                     /locus_tag="VLS86_09460"
     CDS             1268..1780
                     /locus_tag="VLS86_09460"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HSO50763.1"
                     /translation="MFSRAGRASLLDRKPFTEAFFDGDSAADAAILVSTVAAVTYVGR
                     LLVIGEIGAFSFPRLLQVVIAGVVSWLILGFATWFTATRLFGSSGRPQTMIALQGLAV
                     LPLVLELFGRIGGTLGLIWYLVILVVGTREAAEIKTRDAAVSVLIGFAIAAIFRALLN
                     VPFAVFGALF"
     gene            1780..>2274
                     /locus_tag="VLS86_09465"
     CDS             1780..>2274
                     /locus_tag="VLS86_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HSO50764.1"
                     /translation="MPTLRLDLAYDGSGFRGYARQQGLRTVQGVLEDAIATALGSAVE
                     TAVAGRTDAGVHARGQVVSLQVEDEVDVERLARSVNGMVGPEISVTAVTEVASDFNAR
                     FSASWRRYRYSISTGEAPDPLTRGFVWHVGPRIDIEAIVGTAGEFLGEHDFSAFCRSV
                     AGKSN"
BASE COUNT          357 a          760 c          687 g          470 t
ORIGIN      
        1 tcttcctcga acgaggcgcg atcctcgatg tccaacaaca ggacttcgcc ctcggccgcc
       61 acccggaatc cttccgggat ccgtgagatc tctatcgtct tgatatccca gtcgccgatg
      121 acgtgctccc cggaggccac ctgcatccga ccgtcatcga tgtggacggt cgcgggcagc
      181 gcctggttgt cacccggcag tcggagcgtc gctttgaact ttgccatcgc ctgtttatcg
      241 gtcggttttg gctcctactt atggataact atcgcgatct gagaaagagc gattttcggc
      301 ttccggatta gcctgaatgc ccttgagcca tccttcgtat ctcgacttca ccagcgacga
      361 gtgggctgcg ctccgcgcca acaccccgtt gaccctcacc gagtccgacg tcgacaagct
      421 ccggggcatc aacgtcaatc tcgacataga catggtcgag cacagctttc tgccgctctc
      481 ccggctcctg aacctctacg tcctgggaag ccaccagctg gcaaccgtca ccgacacctt
      541 cctcggggcc ccgccccagc cccgaccctt cgtcctgggg gtcgccggat cggtcgccgt
      601 cgggaagtcg accatctccc gcgtcctcca agagctcctc gcccgatggc cgcagcatcc
      661 ccaggtcgag ctggtgacca ccgatgggtt cctgtatccg aatcacgtgc tcgaagagcg
      721 gggattgatg gagcggaagg gcttccccga gagttacgac cggcgggccc tgctcgagtt
      781 cgtcgcccgt tccaagtcgg gcagtgagca tctagaggtc ccggtctact cgcacttcgc
      841 atacgacgtc gttccgggcg agaccagggt gatcagccgc cccgacatcc tgatcctcga
      901 agggctcaac gtgcttcagg cgggcgagaa gggtgcgttc gtctctgact acttcgactt
      961 ctcgatcttc gtcgacgcct cccccgaggc ggtgcgcgag tggtacatcc agcgcttcat
     1021 caccctgcgc gacaccgcct tcgcccagcc cgacgcctac tttcaccgtt acgccaatct
     1081 ctcggacgag gaggcggtgg cggtggcgac cgacatctgg acccggatca acgaggtcaa
     1141 cctcttcgag aacatcctcc ccacccgaga acgggcgacc ttgatcctgg agaaggctcc
     1201 tgaccactcc atcgaacggg tccgtctacg tcgttgaccc ggttagcctc gcccgactgt
     1261 gagcaacatg ttctctcgtg ccggtcgggc ctcgcttctc gatcgtaagc cgttcaccga
     1321 ggcgttcttc gacggcgact cggccgcaga tgcggcgatc ctggtctcga cggtggccgc
     1381 ggtcacctat gtcggtcgtc tcctcgtcat cggggagatc ggggctttct cgtttcccag
     1441 gctgctgcag gtcgtcatcg ccggcgtggt ctcatggttg atcctcggat tcgccacctg
     1501 gttcaccgcc accaggctgt ttggctcgtc ggggcgaccg caaacgatga tcgctctcca
     1561 gggtcttgcc gtgctgcccc tcgtgctcga gctgttcggc aggatcggtg gcaccctggg
     1621 gctgatctgg tacctcgtca tcctggtcgt cggcaccagg gaggcggccg agatcaagac
     1681 ccgagatgcc gcggtctcgg tgctgatcgg atttgcgatc gcggccatct tcagggcgct
     1741 tctcaacgtc ccgtttgcgg tgttcggcgc attgttctga tgccgacgct gcggctcgac
     1801 ctggcctatg acggttcggg tttcaggggc tacgcacgtc aacagggtct gcgcaccgtt
     1861 cagggtgtcc tcgaggacgc catcgcgacc gccctgggat cggcggtgga gaccgcagtc
     1921 gccggtcgca ccgatgccgg cgtccatgcc cgcggtcagg tcgtctcgct ccaggtcgag
     1981 gacgaggtcg atgtcgagcg tctggcccga tcggtcaacg ggatggttgg ccctgagatc
     2041 tcggtcactg ccgtcaccga ggtggcgagc gacttcaacg cgcggttctc ggcctcttgg
     2101 aggcgatacc gctactcgat ctcaaccggc gaagcgcccg acccactgac ccgaggattc
     2161 gtctggcatg tcgggccgag gatcgacatc gaggcgatcg tgggaacggc cggcgagttc
     2221 ctcggtgagc acgacttcag cgccttttgt cgctcggtcg cgggaaagag caat
//
LOCUS       DATITW010000677         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_73869, whole genome shotgun sequence.
ACCESSION   DATITW010000677 DATITW010000000
VERSION     DATITW010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73869"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VLS86_09470"
     CDS             <1..426
                     /locus_tag="VLS86_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50765.1"
                     /translation="SHRRRVSDARRRVPGAHWVVADANDLPFNGATFDRVLLDAPCSG
                     LGTLRRRPEIRFRVTEAEIARLARVQEGMLEKALELVAPGGQLVYSVCTVTPEETIDM
                     VEDRGFEPPPAPGIVWGSGRLLAPHLTGTDGMFIALHRG"
     gene            453..1115
                     /gene="rpe"
                     /locus_tag="VLS86_09475"
     CDS             453..1115
                     /gene="rpe"
                     /locus_tag="VLS86_09475"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HSO50766.1"
                     /translation="MTVVKIAPSLLAADFGCLREEIQGVEAAADWLHIDVMDGHFVPN
                     VSFGIPVIRSIRPLTELYFDCHLMTTNPDAFLPDLAEAGADLVTMHIEAMPDPTRASK
                     LARDNGLDFGLVCNPGTPFSGVAPFLDLCDVLLVMSVEPGFGGQSFMSEVLTKIETAR
                     EWVEERGLATDIQIDGGITPDTARMAKDAGANVFVAGTAIFRAQDPLAAVAELRRAVE
                     EK"
     gene            1112..2041
                     /locus_tag="VLS86_09480"
     CDS             1112..2041
                     /locus_tag="VLS86_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSO50767.1"
                     /translation="MSERVLIADDDPDILAVVQVNFELEGLEVETALDGEDSLQKATA
                     NPPNVIVLDIMMPRMDGLTALHRLRSQASTGNIPIILLTARGLPEDRVRGLELGADDY
                     ITKPFDINELVARVRAVVRRTQAARDLSPLTGLPGNFKITAEIEERITSGRDYALVHG
                     DLDNFKAFNDHYGFMRGDEVIRFCAKCFRDASENLGITGAFVGHIGGDDFVAIIPPEM
                     AESFCKEVIERFDDGILDLYDTADALRGYIEVIDRRGERFAFPVVSLSLGVASTDVRE
                     ISTQWEASAIAVEMKEFAKKQPGSTYRIDRRSA"
     gene            2060..>2359
                     /locus_tag="VLS86_09485"
     CDS             2060..>2359
                     /locus_tag="VLS86_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014002387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HSO50768.1"
                     /translation="MLRAIELATLHRTHPNPRVGAVLVDQAGNVVGEGAHLGVGSPHA
                     EVVALEQAGHRARGSTLYVTLEPCTHHGNTPPCVTAIVTAGVSRVVVGAIDPDERV"
BASE COUNT          422 a          730 c          752 g          455 t
ORIGIN      
        1 tcgcaccggc ggagagtctc ggacgcgcga cgacgggtcc ccggcgcgca ctgggtagtc
       61 gccgatgcca acgacctgcc gttcaacggc gccacgtttg atcgggttct gctcgatgcg
      121 ccatgcagcg gtttgggaac attgcggcga agacccgaga tcaggtttcg ggtcactgaa
      181 gccgaaatcg cccgtttggc ccgagttcag gagggcatgt tggagaaggc tctggagctg
      241 gttgcccccg ggggacagct ggtctactcg gtgtgcaccg tcacccccga ggagacgatc
      301 gatatggtag aggacagggg gtttgaaccg ccccctgcgc ctgggatcgt ctgggggagc
      361 ggtcgtctcc tcgccccgca cctgaccggc accgacggga tgttcatcgc cctccacagg
      421 ggttgaggcc tcccctttac actcaggtac ccgtgaccgt cgtcaagatc gccccgtcat
      481 tgctcgctgc cgacttcggc tgcctccggg aggagatcca gggggtggag gccgccgccg
      541 actggctgca catagatgtg atggatggcc atttcgtgcc caacgtgtcc tttgggatcc
      601 ccgttatcag gagcattcgg ccgttgaccg agctctactt cgattgtcac ttgatgacga
      661 cgaatcccga cgcctttctc cctgatctgg ccgaggccgg agcagatctg gtcacaatgc
      721 acatcgaggc gatgccggac cccactcgag cgtcgaaact ggcacgggac aacggtctcg
      781 atttcggcct tgtctgcaac ccgggcaccc ctttttcggg ggtcgcccca ttcctcgatt
      841 tgtgcgatgt gctgctggtg atgtcggttg agcccgggtt cgggggtcag agcttcatgt
      901 ccgaggtgct gacaaagatc gagaccgcca gagaatgggt tgaggagcgc ggattggcga
      961 ccgatattca gatcgacggg ggcattacgc ccgataccgc acgtatggcc aaggacgcag
     1021 gtgccaacgt gttcgtggcc ggaaccgcga tatttcgggc ccaagatccc ctcgcggcgg
     1081 ttgccgaatt gagaagggca gtagaggaga aatgagcgaa cgggtcctga ttgcggacga
     1141 tgatccggac atccttgcgg tagtccaggt caacttcgag ttggagggac tcgaggtgga
     1201 aacagctttg gatggggagg attctctcca gaaggccacc gccaatccgc cgaacgtgat
     1261 cgtcctcgac atcatgatgc cccgcatgga cggtctcacc gctctgcatc gtcttcgcag
     1321 ccaggccagc accggcaaca tcccgatcat cctgctcacc gcccgcggtc ttccagagga
     1381 ccgggtgcgg ggtctcgagc tgggtgccga tgactacatc accaagccat tcgacatcaa
     1441 cgagctggtc gctcgcgtgc gtgcagtggt tcgccggacc caggccgccc gcgacctctc
     1501 gccgctgacc ggtctccccg ggaacttcaa gatcaccgcc gagatcgagg agcgcatcac
     1561 gtcgggtcgc gactatgcat tggttcacgg cgacctggac aacttcaaag ccttcaacga
     1621 ccactacggg ttcatgcggg gcgacgaagt gattcgcttc tgtgccaagt gcttccgcga
     1681 cgcttcggag aatcttggga tcaccggagc ctttgtcggg cacatcggtg gtgacgactt
     1741 cgtggcgatc atcccgcccg agatggccga gtccttctgc aaggaagtca tcgagcgttt
     1801 cgacgacggg atcctcgacc tctacgacac agcagacgct ctccggggct atatcgaagt
     1861 gatcgaccgt cgcggcgagc gcttcgcctt ccccgtggtg tcgttgtctc tgggtgtagc
     1921 gtccaccgac gtgcgagaga tctccacaca atgggaggcg tcggcgattg cggtggaaat
     1981 gaaggaattt gccaagaagc agccgggctc tacctaccgc atcgatcgtc gctctgcata
     2041 ggtgggcgtg gcccgtcaca tgctgcgagc cattgagctc gccacccttc accggacgca
     2101 tcccaatcca cgcgtaggcg ccgttctggt cgaccaggcg gggaacgtgg tcggtgaggg
     2161 tgcccacctg ggcgtgggca gcccacatgc cgaagtagtt gccctcgaac aggccgggca
     2221 ccgagcgcgt ggctccaccc tctacgtcac cctcgagccc tgcactcatc acgggaacac
     2281 cccgccctgt gtgaccgcga tcgttaccgc cggcgtgagc cgggtggtgg tcggggcgat
     2341 tgatcccgac gagcgggtc
//
LOCUS       DATITW010000678         1714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_73886, whole genome shotgun sequence.
ACCESSION   DATITW010000678 DATITW010000000
VERSION     DATITW010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73886"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VLS86_09490"
     CDS             <1..371
                     /locus_tag="VLS86_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HSO50769.1"
                     /translation="HAREFELIADLGSGTHAARAYARKNGMIVLLKGNPTSVTDGSDT
                     VLLRTGGPELASIGTGDVLAGMIGALWARGLDARTATISGGYWHGVAAADLSRSTTVT
                     ASALAEHVGRFAYSGDGRAR"
     gene            368..1444
                     /gene="alr"
                     /locus_tag="VLS86_09495"
     CDS             368..1444
                     /gene="alr"
                     /locus_tag="VLS86_09495"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HSO50770.1"
                     /translation="MRPTYAEVDLTAVKHNVAAFVALVAPSQVCVVVKADAYGHGDVP
                     IATAALDAGAAWLAVALVAEGIRLREAGIEAPILVLSEPEPGSAPDVAEWDLTPTVYS
                     IPFAEALTATGASLQAHVKVDTGMHRVGAAPEQVDDVIRVLRASHNVEVAALWTHLAV
                     ADEDREFTLRQIELFEEVVARHEAAMTHVANTAGAALFPEARQSLCRIGLGTYGLHPC
                     PQTRDVVDLLPAMRVVSHVSHVQHLEAGARPSYGRIRPLPGQAHVATVAIGYADGYPR
                     RLSAEGRVLIAGQRRPLAGIVTMAQIMVDVGPDVVAVGDEVVLLGSQDGVEVTADEWA
                     RLLGTISYEVVSGIGPRVPRRYVE"
     gene            1441..>1714
                     /locus_tag="VLS86_09500"
     CDS             1441..>1714
                     /locus_tag="VLS86_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HSO50771.1"
                     /translation="MNDTITAVPGVTVGHWSDEEARTGCTVVVFPEPNVASAEVRGAA
                     PGSRETALLEHGMRVQQIQAILLTGGSAFGLAAADGVVRGLEADGRG"
BASE COUNT          259 a          541 c          629 g          285 t
ORIGIN      
        1 ctcacgcccg agagttcgag ctaatcgccg acctgggaag cggcacccac gcagcgcggg
       61 cctacgccag gaagaacggg atgatcgtgc tcctgaaggg aaacccgacc tcggtcaccg
      121 acgggtcgga cacggtgctg ctgcggaccg gggggcccga gttggccagc atcggcaccg
      181 gcgatgtgct ggccgggatg atcggcgctc tttgggcccg ggggctcgat gctcgcaccg
      241 cgaccatctc cggcggttac tggcacggtg tggccgctgc cgatctctcg cgatcgacca
      301 cggtgacggc gagtgcgctg gcggagcacg tcgggcgatt cgcctactcc ggagatggaa
      361 gagcgcgatg aggccgacct acgccgaggt ggatctcacc gcggtcaaac acaacgtcgc
      421 tgcattcgtc gcgctggtgg ctccctcgca ggtctgtgtc gtggtcaagg ccgatgccta
      481 cggccacggc gacgtgccga tcgcgaccgc cgctctggac gcgggtgccg cctggctggc
      541 ggtggcgttg gtcgcggagg ggatccgtct tcgggaggca gggatcgagg ccccgatcct
      601 ggtcctatcc gagcccgagc ccggttccgc tcccgacgtc gccgagtggg acctgacccc
      661 taccgtctat tcgatcccgt ttgccgaggc gctgacggcc accggcgcct cgttgcaggc
      721 tcatgtcaag gtcgacaccg ggatgcatcg agtcggtgct gcgcccgagc aggtcgatga
      781 cgtgattcgg gtgcttcgtg cttcgcacaa tgtcgaagtg gcggcgctct ggacccatct
      841 ggcggtggcc gacgaggacc gagagttcac ccttcgccag atcgagctgt tcgaagaagt
      901 cgtcgcccgc catgaagcgg ctatgaccca tgtcgccaac accgccggcg cggccctgtt
      961 ccccgaggca cggcagtcgt tgtgccggat cggtctcggg acctatgggc tccacccttg
     1021 tccccaaacc cgagatgtgg tcgacctcct gcctgcgatg cgggtggtga gccatgtctc
     1081 ccatgtccag catctcgagg cgggcgccag gccgagctat ggccgcatca ggccgttgcc
     1141 gggacaggct catgtggcga cggtcgcgat cggctatgcc gacggctacc cgagacggct
     1201 ctcggcggag ggccgggtcc tgatcgccgg ccagagacgt ccgctggcgg gaatcgtgac
     1261 catggctcag ataatggtgg acgtcggtcc cgacgtcgtc gcggtcggag acgaggtggt
     1321 gttgctcgga agccaggacg gggtcgaagt gaccgccgac gagtgggccc gcctcctggg
     1381 aacgatctcg tacgaggtgg ttagcggcat tggccccagg gtgccgagga ggtatgtcga
     1441 gtgaacgaca cgatcacggc cgtgcccggc gtcacggtgg ggcactggtc ggacgaggag
     1501 gcgcggaccg ggtgcaccgt ggtcgtgttc cccgagccaa acgtcgcttc ggcagaggtg
     1561 cgtggggcgg ctccgggatc gcgggagacc gccctgctcg agcatgggat gagggtgcag
     1621 cagatccagg ccatcctgct caccggaggg agcgcatttg gcctggccgc ggccgatggc
     1681 gtcgtgcgcg gtctggaagc ggatgggcgg ggcc
//
LOCUS       DATITW010000679         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_794233, whole genome shotgun sequence.
ACCESSION   DATITW010000679 DATITW010000000
VERSION     DATITW010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794233"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VLS86_09505"
     CDS             complement(<1..419)
                     /locus_tag="VLS86_09505"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HSO50772.1"
                     /translation="MQRRFKTWRGGLEVDLSPEEKVFLTELLEVLAAVPGMGDDPASQ
                     RLRVPVYLDDPESNDEWWRLMGEELTTARNDDRRIFQRVLSEPGRARLTHEEADGFLR
                     VLNEGRLALGARFGVEVEEDHDRLPEDRRDILDYLGW"
     gene            complement(419..679)
                     /gene="clpS"
                     /locus_tag="VLS86_09510"
     CDS             complement(419..679)
                     /gene="clpS"
                     /locus_tag="VLS86_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HSO50773.1"
                     /translation="MDPDTDEDQEIDRPWQVIVWNDPVNTMSYVVYVLRKLFGYNETK
                     ATELMLQVHFEGRAIVSDGPREKAEVDCFRLHGHGLWATMEH"
     gene            complement(723..1151)
                     /locus_tag="VLS86_09515"
     CDS             complement(723..1151)
                     /locus_tag="VLS86_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50774.1"
                     /translation="MASAEAISAFRTCLSDAGIEIEEIPIDATGRPRLDLVMASLDFG
                     DPGVAEAVSFCAVHLETGALDLGGEDVLHQAILGHLEDFSRCMVDLGVEGFPEPVPGF
                     LGIGSPFPVAEIPYSDPAFADAVVVCRAALLEALSDVEEE"
     gene            complement(1283..>2858)
                     /locus_tag="VLS86_09520"
     CDS             complement(1283..>2858)
                     /locus_tag="VLS86_09520"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HSO50775.1"
                     /translation="TGLLTEAARISLVRRPDFEMWSFVGYPLSALIAESSAADLHLLF
                     WLVHVASFVGFLVVLPVTKLRHMFTSPANLFLSAHPRPKGAMREMPNLLETDDIETIG
                     ASLVTDLTWKQIFDTDACTICGRCTSVCPANITGKPLDPREMVLKTGEVAAAVAGVSP
                     PVSSLAVLDGEGNLFDRVRPEEVFACVTCRACDEICPVNIEIVDRILDMRRYLTLMES
                     EFPTELGKAFVAMENQSNPWGLSQQTRGDWARELDFEVPVFGENGKTTADYLWFVGCA
                     GSFDDRNTAVSVALARLLHRAGIDFAILGTRELCSGDPARRAGNELVFQQLALQNIAT
                     FEEIGVSRVITQCAHCFNTIANEYSQFGGNYDVVHHSQLLAELLTQGRLQAPTRPDGE
                     VKKIAYHDPCYLGRYNDVYLAPRSVIGASPSVEVVEMPRHGTRALCCGAGGARFWMEE
                     QTGKKINIERAQEALDTEADEIAVACPVCFVMLDDGVKELGRDDVRVRDLAMILADGM
                     ADGMSNDTADEGPGSD"
BASE COUNT          525 a          935 c          910 g          488 t
ORIGIN      
        1 atccagccca gatagtcgag gatgtcacgc cgatcctcgg gcaagcgatc gtggtcctcc
       61 tccacctcga ccccgaaccg ggcgcccagg gcaagtctcc cctcattgag cacccggaga
      121 aagccatcgg cctcttcgtg ggtgagccgg gcccgaccag gctcgctgag gactcgttgg
      181 aagatcctcc ggtcgtcgtt gcgggcggtg gtcagttcct cgcccatgag ccgccaccac
      241 tcgtcgttcg attcggggtc atcgagatag accggcaccc gcagccgctg cgaagccggg
      301 tcgtctccca tcccgggcac cgcggccagg acctcgagga gctcggtgag aaacaccttc
      361 tcctccggcg agagatcgac ctcgaggccc ccacgccagg tcttgaaccg tctctgcatc
      421 aatgctccat cgtcgcccag agaccatgac cgtgaagacg gaagcagtcc acctctgcct
      481 tctccctggg accgtcggag acgatggcgc gcccttcgaa atggacctgg agcatgagct
      541 cggttgcctt ggtctcgttg tagccgaaca acttcctcag cacgtagacg acgtaggaca
      601 tggtgttgac cgggtcgttc cagacgatga cctgccacgg tcggtcgatc tcctgatcct
      661 catcggtatc ggggtccatg accggtgtcg ggctggtagt cacctggacg ttgatcgtag
      721 gcctactcct cttcgacatc agaaagggcc tcgaggagag ccgcccgaca caccacgacc
      781 gcgtcggcga aggcgggatc ggaataaggg atctcagcca ccgggaacgg ggatccgatt
      841 ccgaggaagc cggggactgg ctcggggaag ccctcgaccc cgagatcgac catgcaccgg
      901 ctgaagtctt ccaggtgacc gaggatcgcc tgatggagca catcctcccc cccgaggtcg
      961 agcgccccgg tctcgaggtg cacggcgcag aaggagacag cctcggccac ccccggatcg
     1021 ccgaagtcga ggctcgccat gacgaggtcg agacggggac ggccggtggc atcgatcggg
     1081 atctcctcga tctcgatccc ggcgtcgctg agacacgtcc ggaaggcgct gatcgcctcg
     1141 gccgaggcca cggtggtcgt ggtcgtggga gccgtggtgg tggtggttgc agggacatcg
     1201 gtggccgacg actggtcggc atcaggggtg cacgccccca gaagcagcgc cgccagccac
     1261 gcaatgagag ccgcccgtcg gatcagtcgc tccccggccc ttcgtcggcc gtgtcgttgc
     1321 tcatcccatc ggccatccca tcggcaagga tcatcgccag atcccggacc cggacgtcat
     1381 ctcgacccaa ctccttgacc ccgtcgtcga gcatcacgaa gcagacggga caagccaccg
     1441 caatctcgtc ggcctcggtg tccagtgctt cctgcgcacg ctcgatgttg atcttcttcc
     1501 cggtctgctc ctccatccag aagcgcgctc cgcctgcgcc acagcacagc gccctggtcc
     1561 cgtgccgagg catctccacc acctccaccg agggggaggc accaatcacc gaacgtggag
     1621 cgagatacac gtcgttgtat cgccccagat agcaggggtc gtggtaggcg atcttcttga
     1681 cctcaccgtc gggccgggtc ggcgcctgga gacgaccctg ggtcaggagc tcggcgagaa
     1741 gctggctgtg gtggacgacg tcgtagttgc caccgaactg tgagtactcg ttggcgatgg
     1801 tgttgaagca atgagcgcat tgcgtgatca cccggctcac acctatctcc tcgaacgtgg
     1861 cgatgttctg cagggccagc tgctgaaaca ccagctcgtt gccggcgcgt cgcgccgggt
     1921 cgcccgagca gagctcgcgg gttccgagga tggcgaaatc gatcccggct cggtgcagta
     1981 gccgtgccag cgccaccgac accgcggtgt tgcggtcgtc gaaggacccg gcacagccca
     2041 cgaaccagag atagtcggcc gtggtcttgc cgttctcccc gaagaccggc acctcgaagt
     2101 cgagctcccg ggcccagtct ccccttgtct gctggctcaa accccaaggg ttggattgat
     2161 tctccatggc tacgaacgcc ttgcccaact cggtcgggaa ctccgactcc atgagggtga
     2221 gatagcgccg catgtcgagg attcgatcga cgatctcgat gttgaccggg cagatctcgt
     2281 cgcatgcccg acaggtcacg caggcgaaga cctcctcggg cctgacccgg tcgaagagat
     2341 tgccctcacc gtcgagaact gcaagcgagg agaccggtgg ggataccccc gctactgcgg
     2401 cggccacctc gccggtcttg agcaccatct cccgagggtc gagcggcttg ccggtgatgt
     2461 tggccgggca caccgaagtg catctgccac atatggtgca agcgtcggtg tcgaagatct
     2521 gcttccaggt gagatcggtt accagcgagg cgccgatggt ctcgatgtca tcggtctcga
     2581 ggaggttcgg catctcccgc atcgcgccct ttggcctggg gtgagcggag aggaagagat
     2641 tggccggcga ggtgaacatg tgtcgcaact tggtcacggg gaggaccacc aggaacccga
     2701 cgaacgacgc cacgtggacc aaccagaaca gaagatggag atcggcggcg ctcgactccg
     2761 cgatcagcgc cgacagcgga tagccaacga acgaccacat ctcgaagtcg ggacgacgga
     2821 ccagggagat gcgggccgcc tcggtgagta gtccggtg
//
LOCUS       DATITW010000680         3315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_153407, whole genome shotgun sequence.
ACCESSION   DATITW010000680 DATITW010000000
VERSION     DATITW010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153407"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2106)
                     /locus_tag="VLS86_09525"
     CDS             complement(<1..2106)
                     /locus_tag="VLS86_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSO50776.1"
                     /translation="MPVLNNKVRFVTATTLFDGHDASINIFRRILQASGAEVIHLGHN
                     RTGLDIVETAIEEDAHAIAVTSYQGGHMEFYRYLHDLLVERDATHIRLFGGGGGTILP
                     AEGDQLTEYGITRVYSPDDGRALGLQGMVSDMLSRSDFSTGELGDDIDLDELGPGNRK
                     LVARLISAAENYSEKYPEIFEQIEERARQASAPVVGVTGTGGSGKSSLVDELLRRFLS
                     DFEDLRVAVISVDPSKRRSGGALLGDRIRMNSLPHPRAYMRSLATRQANLALSPHIQH
                     ALNVVKAAGYDLVVLETSGIGQSDTEIVDLSDLAVYVMTPDYGAATQLEKIDMLDFAD
                     LVAINKADKEGALDALRDVRKQYKRNTLAFDAGDESLPIHLTVASDFNDPGTNRFYRD
                     FVGRLGAHGLELESTLDLPVSDEAKLHVVPPERTRYLAEIVEAIREYDSWAAEQAAVA
                     DTLYRLEGARQAGADVAEILAATAQELDPENLRALEHWDAMVAEYAGQEFVYHVRGNE
                     VRVPLHHETLSHTRIPKVALPRYRSWGDRLHWLLQENVPGEFPYAAGVFPFRRIEEDP
                     ARMFAGEGPPEQTNRRFHYLAAGQPAKRLSTAFDSVTLYGEDPHERPDVYGKVGNSGV
                     SIATLDDAKKLYSGFDLSDPATSVSMTINGPAPMILAFFMNAAVDQACESHITENAMW
                     DQVESRIDDILGSDPRPVYR"
     gene            2219..2527
                     /locus_tag="VLS86_09530"
     CDS             2219..2527
                     /locus_tag="VLS86_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50777.1"
                     /translation="MAYYIGRLQGPKLEPTVVTVEIDDGRLRIVAGRRHMGSWPLPTM
                     DILRTSIYRFAFEIEGDNFEFFPDDPSAFSDAAGAIVDLTGREGRYGLKARIQDATSS
                     "
     gene            2553..3299
                     /locus_tag="VLS86_09535"
     CDS             2553..3299
                     /locus_tag="VLS86_09535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSO50778.1"
                     /translation="MVPRLLRHGVTAGAVLLGAEAAYAVLRPVPKLSDFDPSGSFGES
                     HLPSLRVAVLGDSSVTAPGVAGPEEIWVSRVCSRLSETSYVTLESFAVSGSKAADLIE
                     NQLEPTLAFDPDLVLVAVGANDVIRGVSVRAFEGQLDDLIGALAETDALVIMSGVGDM
                     GTIPRLHPPLRNLMTRRSQRFNEIHLEVAARYGAYVIDQRSDDPDSWYTDRSLWSPDL
                     FHVSAAGHERWANLTWRTIEPLLGVRHGKG"
BASE COUNT          572 a         1063 c         1065 g          615 t
ORIGIN      
        1 gcgatacacc ggtctcgggt cggagccgag gatgtcgtcg atcctcgact caacctgatc
       61 ccacatcgcg ttctcggtga tgtggctctc acaagcctgg tcgacggcag cgttcatgaa
      121 gaaggcgagg atcatcggag ccggcccgtt gatcgtcatc gagaccgagg tagcgggatc
      181 ggagagatcg aacccggagt acagcttctt tgcgtcgtcg agcgtggcga tcgagactcc
      241 ggagttgccg accttcccgt agacatcggg ccgctcgtgc gggtcttcgc cgtacagcgt
      301 caccgagtcg aaggcggtcg acagacgctt cgccggctgg cctgccgcca gatagtgaaa
      361 ccgccggttg gtctgttccg gggggccctc accggcgaac atccgcgccg ggtcttcctc
      421 gatccgccgg aacggaaaga ccccggcggc gtaggggaac tcaccgggta cgttctcctg
      481 aagcagccag tggagacggt ccccccagga tcgatatcgg ggcagggcca ccttggggat
      541 ccgggtgtga ctcagcgtct cgtgatgaag cggcaccctg acctcgttgc ctctcacgtg
      601 gtagacgaac tcctggccgg cgtactcggc gaccatcgcg tcccagtgct cgagagcgcg
      661 gaggttctcg ggatccagct cctgagccgt ggcggcgagg atctcggcga catcggcgcc
      721 tgcctgacgc gccccttcca ggcggtagag ggtgtcggcg acagcagcct gctcggctgc
      781 ccaggagtcg tactcgcgga tcgcctccac gatctcggcc aggtacctgg tgcgctccgg
      841 cggcaccacg tggagcttcg cctcgtcact caccgggaga tcgagagtcg actccagctc
      901 gagcccgtgg gcaccgaggc gcccgacgaa gtcccggtag aagcggttgg tccctgggtc
      961 gttgaagtca gaggcgacag tcaggtggat aggcagcgac tcgtcaccgg cgtcgaaggc
     1021 gagagtgttg cgcttgtatt gcttgcggac gtccctcagg gcatccaatg ctccctcttt
     1081 gtccgccttg ttgatcgcca ccaggtcggc gaagtccagc atgtcgatct tctccagctg
     1141 agtcgccgcg ccgtagtccg gggtcatcac gtatacggcg aggtcggaga gatcgacgat
     1201 ctcggtgtcg gactgcccga tgcccgacgt ctccaggaca acgaggtcat agcctgcggc
     1261 tttcaccacg ttcagtgcgt gctggatgtg gggagagagg gcgagattcg cctgacgtgt
     1321 cgccagcgac cgcatgtagg cccgggggtg gggcagggag ttcatccgga tccggtcacc
     1381 gagcagagcc ccaccggatc ttcgcttgga tgggtccacc gagatcacgg cgactcgcaa
     1441 atcctcgaag tcgctgagaa agcgacgcag caactcgtcg accagactcg acttgcccga
     1501 gcccccggtg ccggtgactc cgaccaccgg tgccgacgcc tgcctggccc tctcctcgat
     1561 ctgctcgaag atctccgggt acttctcgga gtagttctcg gcggccgata tcagcctggc
     1621 caccaacttc cggttcccgg gtccgagctc atcgagatcg atgtcgtcgc ccagctcgcc
     1681 ggtggagaag tcggaccggc tcagcatgtc gctcaccatc ccctggaggc ccagggcgcg
     1741 accatcgtcg ggcgagtaga cgcgagtgat cccgtactcc gtgagctggt cgccctccgc
     1801 ggggagaatg gtccctccgc ccccgccgaa gagccggatg tgagtcgcat ccctctccac
     1861 caggagatca tggagatagc gatagaactc catgtgaccc ccctggtagg aggtcaccgc
     1921 gatggcgtgg gcatcctcct cgatggcagt ctccactatg tccagcccgg tgcggttgtg
     1981 accgagatgt atcacctcgg cgcccgaggc ctggaggatg cggcgaaaga tgttgatcga
     2041 ggcgtcgtga ccgtcgaaga gagtggtggc ggtcacgaat cggaccttgt tgttcaatac
     2101 gggcatggtg gacgcggccg tgatgcagcc ttcccatgga ctagctcaaa ctgtaacccc
     2161 agattgggag ttttccccct gttcactatc cacaagaacg actacattgg ataacgggat
     2221 ggcttattac atagggcgct tgcaggggcc gaaactcgag cccaccgtgg ttaccgtgga
     2281 aatcgacgac gggcgccttc gcatcgtcgc cgggcgacgt cacatgggtt cgtggcctct
     2341 tcctaccatg gacatcctcc gcacctcgat ctatcgcttc gcgttcgaga tcgagggcga
     2401 caacttcgag ttcttccccg acgacccatc ggcgttctcc gacgccgcgg gggcgatcgt
     2461 cgacctcacc ggccgcgagg gcagatacgg cctcaaggcc cgaattcagg acgcaacgag
     2521 ctcctgaacc aagaaccctg gcgcagttga ccatggttcc ccgactgttg aggcacggag
     2581 tcaccgcagg cgccgtgctc ctcggagccg aagctgccta cgccgtgctc cgcccggtcc
     2641 cgaagctgtc cgatttcgac ccttccggca gttttggtga gtcacatctc ccgtcactgc
     2701 gggtcgcggt cctcggcgac tcctcggtga ccgcccctgg cgtggcaggc ccggaggaga
     2761 tctgggtgtc tcgggtgtgc agccgtctct ctgagaccag ctacgtgacg ctggagtcgt
     2821 ttgcggtcag cgggtcgaag gcggcggacc tgatcgagaa ccagctcgag ccgactctgg
     2881 cctttgaccc cgacctggtc ctggtggcgg tcggagccaa cgacgtgatc cgtggcgtga
     2941 gcgtcagggc cttcgagggc cagctcgacg atctgatcgg ggcgctggca gaaaccgatg
     3001 ccctggtgat catgagtggt gtcggcgaca tgggcaccat cccccggctc cacccacccc
     3061 ttcgcaacct gatgacgaga cgctcgcaga gattcaacga gatccacctc gaggttgctg
     3121 cccgctacgg ggcctacgtg atcgaccaga ggtccgatga cccggattcc tggtacacgg
     3181 accgaagcct ctggtctccc gacctgttcc acgtgagcgc agccggccac gaacggtggg
     3241 ccaatctgac ctggcgaacg atcgagccac ttctcggcgt gcgtcatgga aagggctgag
     3301 gcgctcgatc gtctc
//
LOCUS       DATITW010000681         1549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_33837, whole genome shotgun sequence.
ACCESSION   DATITW010000681 DATITW010000000
VERSION     DATITW010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33837"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..902)
                     /locus_tag="VLS86_09540"
     CDS             complement(138..902)
                     /locus_tag="VLS86_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO50779.1"
                     /translation="MRPDNSLPLLSVRSGTKRFGGVLALDEMDVNVFAGEVLAILGDN
                     GAGKSTLIKCMSGVHRLDTGVIEVDGDPVQLHSPSDARGLGIETVYQDLALFDNLSPS
                     ANFYAGRELAGPRWLPMGMRLMQRRTMSQATQDVLDRLQVTLPDFEAPVGLMSGGQRQ
                     AVAVARATAFASRLVILDEPTAALGVRESARVLDLIRRLRDEGKAVVVISHAMDHVME
                     VADRAVVMRRGRNVGELVPSESSRERMVSLIVGGEG"
     gene            complement(899..>1549)
                     /locus_tag="VLS86_09545"
     CDS             complement(899..>1549)
                     /locus_tag="VLS86_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO50780.1"
                     /translation="LFVKGRIPHPFIVTLATFTLVRGVALWLSDGRPIQEMPTVVQVI
                     GGDTILGWIPVSSFVVAGVAVAVWMLTTRVVWGRWIYAVGGNPEAARRTAIPISTILI
                     SVYVFSGLTAGIAGLLTAGRTNAGAPTFGDLAELDAIAAVIIGGASFLGGRGKVSHAL
                     VGALMIGVIRNAMNLANLDSFFQLIVLGIVIIVAVESDVLRGHLENKLRLRQATRT"
BASE COUNT          300 a          532 c          452 g          265 t
ORIGIN      
        1 cccaccacaa gtaccgccca ccggcgcact gtctttgaat tcatcccgtt cctcctgttg
       61 tctgttgggt ggaatcgttt ctctgcaccc ggcgccgcca cccaggtcgg caccgggaac
      121 caaggcaagt caatccgtca accctcacct ccaacgatca gagagaccat tcgctctcga
      181 gagctttctg aagggaccaa ctcgcccacg ttgcgtcccc ggcgcatcac taccgcccga
      241 tcggccacct ccatcacgtg gtccatcgca tgggagatga cgaccaccgc tttcccttcg
      301 tcccgaaggc gtctgatgag gtcgaggacc cgtgcagatt cacgcactcc gagagcggct
      361 gtcggctcgt ccaggatgac aagccgtgag gcgaaggcgg tagcccgcgc caccgccacc
      421 gcttgacgct gccctccgga catcaatcca accggtgcct cgaagtcagg cagggtgacc
      481 tggagacggt cgaggacatc ttgtgtcgcc tgcgacatgg tccgtctctg catcagccgc
      541 atccccatcg gcagccagcg cggcccggcc agctcccgac cggcgtagaa gttggcggat
      601 gggctcaggt tgtcgaagag cgccaggtcc tgatagacgg tctcgatgcc gaggccacga
      661 gcgtcggagg ggctgtgcag ctgcaccggg tcgccgtcga cctcgatcac ccccgtgtcg
      721 aggcgatgga ctccgctcat gcacttgatc agggtcgatt tgccggcacc attgtcccct
      781 aggatcgcca gcacctcccc ggcgaagaca ttgacgtcca tctcgtcgag ggcgagcacc
      841 ccgccgaagc gcttggttcc actccgcacg ctcagtaggg ggagagaatt gtccggtctc
      901 atgttctggt cgcctgtcgc agacggagct tgttttccag gtggccgcgc aacacgtcag
      961 actcgacggc cacgatgatc acgatcccaa ggacgatcag ctggaagaac gaatccaggt
     1021 tggccaggtt catggcgttt ctgatcacgc cgatcatcaa cgccccgacc agggcgtgac
     1081 tcacctttcc ccgcccaccc agaaagctcg cgccgccgat gatcactgcc gctatggcat
     1141 cgagctcggc caagtcgcca aaggtggggg cgccggcatt cgttcgaccc gcggtgagca
     1201 agccggcaat gccggcggtc aagccgctga aaacatagac cgagatcagg atcgtcgaaa
     1261 tcgggatcgc cgtccgacgc gctgcctccg gattgccgcc gaccgcgtag atccagcggc
     1321 cccagacgac acgggtggtc aacatccaca ctgccactgc cacgccagca acgacaaacg
     1381 atgacactgg gatccatcca aggatcgtgt ccccgccgat cacctggacc accgtgggca
     1441 tctcctggat ggggcggccg tccgagagcc acagggccac tccccgcacc agcgtgaacg
     1501 tggcgagtgt gacgatgaac gggtgcggta tccgtccttt cacgaaaag
//
LOCUS       DATITW010000682         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_393949, whole genome shotgun sequence.
ACCESSION   DATITW010000682 DATITW010000000
VERSION     DATITW010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393949"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VLS86_09550"
     CDS             complement(<1..959)
                     /locus_tag="VLS86_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSO50781.1"
                     /translation="MAVLAVLVSACAGSTEDTTTTAAAEETTTTAAAGDTTTTGEAPA
                     QGAMTMTVDINPDAVWSDSTPITVADFACTRQANLNTPGAISTAGWTEILSVEPGTSD
                     KQVVVEFSTVYAPYKNTFNQILPSHLIADCNDVSADFLDNIPVSARPWQLQEWSDQQA
                     ILVPNEAYWGDDTAVASRLVQVPRTEDGGIAALGAGEVDFIFPQAFAGITDALAADNI
                     TFEPGYGTNYEGLYFQQGADRGGPFSDPVFREAFSKSVDRQLILTNINEPALPGAPLL
                     ECGLWVPTVGPWCDNTQFVDSYDPEGAVALLEGDGWTLNGDGF"
     gene            complement(1213..>2307)
                     /locus_tag="VLS86_09555"
     CDS             complement(1213..>2307)
                     /locus_tag="VLS86_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSO50782.1"
                     /translation="LPMAESHDRQDNRTFGRRPVVVAGVLVATGVAAYLLFQIRSLLF
                     MIFVAAFIAVAIEPPVHFLEKRGWKRGAATGLVFLVGFALFVVFSVALAPLFVGQLND
                     LIEALPGYIESVSLLAQDWFGLDLSTEALQEEAAGLPEIISGAGGTIVGGVVSITTGI
                     ASFLFFALTVALFSFYMVAELPQLQRTILSTMPEERQHDLMHVWDVAVEKMGSYIYSR
                     LILAALSGTLTVIVLSILEVPFAVSLGIWVGVLSQFIPVVGTYLAAILPAIVALSSQG
                     TTTMLWVIGFFLAYQLVENYLIAPRITKRTMEIHPAVSIGAIIVGSQLLGPIGIILAL
                     PMAGIIQALISESRRRHEVILDNPKNEAKK"
BASE COUNT          459 a          711 c          742 g          395 t
ORIGIN      
        1 cagaagccgt cgccgttcag ggtccagccg tcaccctcga gcagtgcgac tgcaccctcg
       61 gggtcgtagc tgtccacgaa ctgggtgttg tcacaccagg ggccaaccgt cggaacccag
      121 aggccacact cgagcaacgg ggctccgggc agagcgggct cgttgatgtt ggtcaggatc
      181 agttggcggt cgacggactt gctgaaggcc tcacggaaca ccgggtcaga gaacgggcca
      241 ccgcggtccg caccctgctg gaagtagagg ccctcgtagt tggtgccgta tccaggctcg
      301 aaggtgatgt tgtccgcagc caacgcatcg gtgatgccgg caaatgcctg ggggaagatg
      361 aagtcgactt caccagcacc caaagcagcg atgccaccgt cctccgtgcg cgggacctgg
      421 accagacggc tcgcaaccgc agtgtcgtca ccccagtacg cctcgttggg aacgaggatc
      481 gcctgctggt cactccactc ctgcagctgc catggccggg ccgacaccgg gatgttgtcc
      541 aggaagtcgg cagacacgtc gttgcagtcc gcaatcaggt gcgaaggcag gatctggttg
      601 aacgtgttct tgtagggggc atagacggtc gagaactcga caactacctg cttgtcgctc
      661 gtccctggct ccaccgacag gatctcagtc catccggcag tcgagatcgc tccgggtgtg
      721 ttcaggttcg cctgcctggt gcacgcaaag tcggcaacgg tgatcggagt gctgtcactc
      781 cacaccgcgt cggggttgat gtcgactgtc atcgtcatcg caccctgggc cggagcctca
      841 ccggtcgtcg tcgtgtcgcc ggcagccgcc gtggttgtcg tctcttcggc cgccgcagtg
      901 gtggtggtgt cctcggtgga gccagcacaa gcgctgacca acacggcgag aacggccacc
      961 aatgcgatcc atcgagaacg attcctcatt cttttcattg attcccttcc tgctttattg
     1021 cattgccatg gtcgggaccg agcccgagcc acgtgggaaa gacggacgaa tccgcacctc
     1081 cccgccagag gtcgagggtg atggcacctg cccggccagt ggacgcgcgg attgtatacc
     1141 ctgaccgttt cctccgcacc taacgcgccg cccatatcgc ggaccggcac cgcgatctca
     1201 cgagcgtcac ggtcacttct tcgcctcgtt ttttggattg tcgaggatca cttcgtgacg
     1261 acgtctcgac tccgagatca gggcctggat gattcccgcc atggggaggg cgaggatgat
     1321 tccgatcggg cccaggagct gggacccgac gatgatggcg ccgatcgaga ccgcggggtg
     1381 gatctccatc gtcctcttgg tgattcgagg agcgatcagg taattctcca ccaattggta
     1441 ggcgaggaag aacccgatca cccagagcat ggtcgtcgtg ccctgggacg acaaggcgac
     1501 gatggcgggg aggatcgcag ccagataggt gccgacgaca gggatgaact ggctgaggac
     1561 accgacccag attcccaggg agaccgcgaa gggcacctcg aggatggaga ggacgatcac
     1621 ggtgagagtg ccgctcagag ccgccaggat cagcctcgag tagatgtagc tgcccatctt
     1681 ctccaccgcc acgtcccaca catgcatcag gtcgtgctgg cgctcctcgg gcatcgtcga
     1741 caggatggtc cgctgcagct gggggagctc ggcgaccatg tagaaggaga acagggcaac
     1801 cgtcagcgcg aagaacaaga agctggcgat gccggtcgtg atgctgacca ctcctccgac
     1861 gatcgtgcct ccggcgccgg agatgatctc gggcagcccg gcagcctcct cctggagcgc
     1921 ctcggtggag agatcgaggc cgaaccagtc ctgagccagc agagacacag actcgatgta
     1981 gccgggcagt gcttcgatca ggtcgttcag ctggccgacg aagagagggg ccagcgccac
     2041 agagaacacc acgaacaggg cgaaaccaac caggaacacc aaaccggtgg cggcgccccg
     2101 cttccaaccc cgcttctcga gaaagtgcac cggaggctcg atggccactg cgatgaaagc
     2161 ggcgacaaag atcatgaaga gcagcgagcg gatctggaac agcaggtagg cggcgacacc
     2221 ggtcgccacc aggaccccgg ctaccacgac gggtctccgg ccgaaggttc gattgtcctg
     2281 gcggtcgtga ctttcggcca ttgggag
//
LOCUS       DATITW010000683         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_674157, whole genome shotgun sequence.
ACCESSION   DATITW010000683 DATITW010000000
VERSION     DATITW010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674157"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VLS86_09560"
     CDS             complement(<1..877)
                     /locus_tag="VLS86_09560"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF014553.4"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HSO50783.1"
                     /translation="MESFNPENYLNRELSWLEFNQRVMLLASRPDFPLLEQVKFLAIW
                     ASNLDEFFQVRVAGLKEQVAHGVGHTPPDGMTPSQQLAAIRRDVDLQYEAISKQFLSE
                     IAPALAEEGIVFSDYSTLDPEDRDFLDHQFRELVYPVLTPLAVDPSHPFPYISNLSLN
                     MAVFVRNPETGLARFARVKVPPILPRFVVLPDLERFVPLEQVMAAHLDALFPGMEVLS
                     NHVFRVTRNADFEVEEDEGGDLLMAIESELTRRRFGSVVRLEIHPDMPEDVLDLLMRE
                     MGATEDDVYRIEGPLD"
     gene            complement(917..1531)
                     /locus_tag="VLS86_09565"
     CDS             complement(917..1531)
                     /locus_tag="VLS86_09565"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSO50784.1"
                     /translation="MPRIRATSIDEHKALTRSALIESAKELIATAGTAEVPLGEVALA
                     AGVGRTTLYDYFTDRDDLIATMVEEELPGVVDDLISTSSRIGPAQLRLADLAVRTVEF
                     VVNDPVLGLILHREVGRMGLEAQQRIRAAHSNLADAMVGLYYEGVEKGEFRSMPPELA
                     GRLIQDTIMSAARTVISSRAPEDTIGLVTGHLRRFLVGGLSYER"
     gene            1626..>2416
                     /locus_tag="VLS86_09570"
     CDS             1626..>2416
                     /locus_tag="VLS86_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50785.1"
                     /translation="MGNEQGQIETAVVVKEARPFTGFFLGLLFGVALAVILQQAGVWP
                     LDRLLLFGAAGIFSLIGILIAGAGRAKVGPFSSILPLLLAVGLIGIGATGLTTLNETG
                     ELNGGCTVQATSSLDETVVTDTSRQDPFVIDPEGSLSWVATSPGPITDHLWNIYVDVG
                     GFSVRIAGNDEPEPNTDGDQENVGDVADVSAYVREVSDFTGVELRGVFEVAGDIEGGG
                     GACDGFGFVRLEADPLTTIISQIAAGVALLALIGLGAIAFNRTREA"
BASE COUNT          438 a          761 c          782 g          435 t
ORIGIN      
        1 gatcgagagg cccttcgatc cggtagacat cgtcctcagt cgcacccatc tcccgcatca
       61 gcaggtcgag cacgtcctcg ggcatgtcgg ggtggatctc caaccgaacc acgctcccga
      121 aacgacgacg ggtcaactca gattcgatcg ccatcagcag atcgccgccc tcgtcctcct
      181 cgacttcgaa gtcggcgttg cgggtcaccc gaaacacgtg gttgctcagc acctccatcc
      241 ccgggaagag agcatcgaga tgggccgcca tcacctgctc caagggcacg aagcgctcga
      301 ggtcgggcag gaccacgaag cgagggagga tgggtgggac cttgacccgg gcgaacctgg
      361 ccagcccggt ctccgggttt cgcacgaaga ccgccatgtt gagcgacagg ttggagatat
      421 aggggaaagg atgggatgga tcgaccgcga gcggagtcag caccggatag accaactccc
      481 gaaactgatg atcgagaaaa tcccggtctt cgggatcgag ggtcgagtaa tcgctgaaga
      541 cgattccctc ttctgccagg gccggagcga tctctgacag gaactgcttg gagatcgcct
      601 cgtactggag atcgacatcg cgtcggatcg cggccagttg ctggctgggt gtcatcccgt
      661 ccggcggcgt gtgcccgacg ccatgggcca cctgctcctt gagcccggcg acgcgcacct
      721 ggaagaactc gtccagattc gaggcccaga tcgccaggaa cttgacctgt tccagcaacg
      781 ggaagtcggg tctcgaggca aggagcatga cccgttggtt gaactcgagc caggacagct
      841 cccgattcag gtaattctct gggttgaagg attccacttt gtcccgattg actagcaggt
      901 ctgatttacc aaacggttaa cgctcgtagc tgagccctcc cacgaggaag cgccggagat
      961 ggcccgtgac caagccgatc gtgtcctccg gggcccggga cgagatcacg gtcctggctg
     1021 cggacatgat cgtgtcctgg atcaggcgcc cggccagctc gggtggcatc gacctgaact
     1081 cccctttctc gactccttcg tagtagagcc cgaccatggc atcggccagg ttggaatggg
     1141 cggcacggat gcgctgctgt gcctcgaggc ccattcgccc cacttcacga tggaggatca
     1201 agccgaggac cgggtcgttg acgacgaatt cgacggtcct cactgcgagg tcggcgagtc
     1261 tcagctgtgc agggccgatt cgggatgagg tggagatcag gtcgtccacc acgccgggca
     1321 gctcttcctc gaccatggtt gcgatcaagt cgtcccggtc ggtgaagtag tcatagaggg
     1381 tggtccggcc cactcccgcg gcaagggcga cctcacccag aggcacctcc gcggttcccg
     1441 ccgtggcgat cagctccttc gccgactcga tcagggcact acgggtcagc gccttgtgct
     1501 cgtcgatcga ggtcgcgcga atccggggca tgaccagagg ctatatcgac cgggcgcaaa
     1561 caaccatcgg atgtcgacac cgcgcccccc tttccgcgta ggttcggtgc gacgagagga
     1621 gttccatggg caacgagcaa ggacagatcg agacggctgt ggtggtgaag gaggccaggc
     1681 cgttcaccgg cttcttcctc ggactgctgt tcggggtcgc actggccgtc atccttcagc
     1741 aggccggggt ctggccgctg gaccgcctgc tcctcttcgg ggcggccggg atcttctccc
     1801 tgatcgggat cctgatcgcc ggcgcgggcc gggccaaggt tggcccgttc tccagcatcc
     1861 tccccctcct cctggcggtc ggcctcatcg gaatcggggc gaccggcctg accaccctga
     1921 acgagacggg ggagctcaac gggggatgca cggtgcaggc gacctcgagc ctcgacgaaa
     1981 cggtggtcac cgacaccagc cggcaggatc cattcgtcat cgatcccgag gggagtctct
     2041 cctgggtggc gacctccccg ggaccgatca cggatcacct ctggaacatc tacgtcgatg
     2101 tcggcggatt ctcggtgcgt atcgccggga atgacgagcc ggagccaaac accgacgggg
     2161 accaggagaa tgtcggcgac gtcgccgatg tgtcggccta cgtccgggag gtttccgact
     2221 tcaccggtgt cgagttgcgt ggtgtcttcg aggtggctgg ggacatcgaa ggtggaggcg
     2281 gcgcctgcga cggtttcgga ttcgttcgcc tggaggccga tcccctgacc acgatcatct
     2341 cccaaatcgc ggccggtgtc gcccttctcg cgctgatcgg cctcggcgcc atcgccttca
     2401 acaggaccag agaggc
//
LOCUS       DATITW010000684         3047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_474141, whole genome shotgun sequence.
ACCESSION   DATITW010000684 DATITW010000000
VERSION     DATITW010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474141"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VLS86_09575"
     CDS             <1..449
                     /locus_tag="VLS86_09575"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HSO50786.1"
                     /translation="ATELPRHDVKPEVEVFHSGAVWNLNKLHSEGLLAEPVWATLFID
                     WAGGSWTPPSEAAIIFMTDLLPDFVNYNVSVMSPTTQWKLLSMVIGLGGHVRVGAEDN
                     PYLRPGELADSNARLVEKIVRIAGEHGREIATPAEARQIIALTGAG"
     gene            455..1384
                     /locus_tag="VLS86_09580"
     CDS             455..1384
                     /locus_tag="VLS86_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HSO50787.1"
                     /translation="MAKLVVSEFLQEHYLDLLRASHEVVFDPDMYSDRPRLLGEVGEA
                     EAIFIRNRTRIDEELIAAATRLRVVGRLGVGLDNIDMEACARVGIEVIPAVGANAVAV
                     AEYVMGAMLVLTRGVFDMTPSMVAGEWPRQGHAFGHELMGMTLGLIGFGAIAREVANR
                     ATAFGMRIVAFDPFVPESDPVWRTVARLSFEEVLAESDVVSLHAPLTEATRNLIGESA
                     LALMKPNAVLINTSRGGTLDETAVADALRRGVISGAAIDVFESEPLGPEPASTFAAVP
                     NLLLTPHVAGNTHEAVDRVARMIVSRVLEAIQR"
     gene            complement(1417..2676)
                     /locus_tag="VLS86_09585"
     CDS             complement(1417..2676)
                     /locus_tag="VLS86_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HSO50788.1"
                     /translation="MHHLRSPGVGRADITGYAKGPFAVDWEDRYDFGELRRQRVDKAQ
                     TALRASDADAILVWKDENVRYLTSLRAQLIAGKTTSLNGALLTADADPVLLGSGGEID
                     KARFGMSWIGEAHAVPIMEQRELVDGFVKNILTPLLETRALTSGRLAVDQANISFIQS
                     MRAHFPDLELIDGDTVMQQARWIKTDLEVAVIEEACAIGDSVTQRALDETRAGRRELD
                     IAADAMQTLFQLGGEMAHVITPFVASGEHMSPPHRLATDKIIRNQDLCFIDIGAMWNG
                     YFADIGRTTIVGRPSKQQQKIYTAVYEGLMAGLEQMRPGNTNQDCADAIIAKIADHGL
                     DDHLFSLFIGHGIGMGANEPPYIGETLPGATTYEFQPNMVFAVEPLVWVPNVRGGGGV
                     RLEDMVLITEGDPHLLSRVEYEERLLI"
     gene            2760..>3047
                     /locus_tag="VLS86_09590"
     CDS             2760..>3047
                     /locus_tag="VLS86_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005257390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSO50789.1"
                     /translation="MTSQDSLRLDATTSRRITAVDLVRDALRAAILRGDLPGGSRLVQ
                     TEIAAQLKVSTTPVREAMRDLSSEGLITLDSHRIGTVRKPDWDEMVEIVEIR"
BASE COUNT          529 a         1001 c          970 g          547 t
ORIGIN      
        1 acgcgacgga attgccccga cacgacgtga agcccgaggt cgaggtgttc cactccggcg
       61 cggtctggaa cctgaacaag ctccactccg agggtctgct cgccgaaccg gtgtgggcca
      121 ctctgttcat cgattgggca ggcggctcct ggaccccgcc gagcgaggcc gcgatcatct
      181 tcatgaccga tctgttgccc gatttcgtca actacaacgt cagcgtcatg agccccacca
      241 cccaatggaa gctgctctcc atggtcatcg gcctgggagg ccacgtccgg gtgggcgccg
      301 aggacaaccc ctatctcaga cccggcgagc tcgccgacag caacgcccga ctggtggaga
      361 agatcgtgcg catcgccggc gagcacggtc gtgagatcgc caccccagcc gaggcgagac
      421 agatcatcgc tctcaccggg gccggatagc caccatggcc aagctcgtcg tgtcggagtt
      481 cctccaggag cactatctcg acctgctccg cgccagccac gaagtggtgt tcgacccgga
      541 catgtactcc gatcgccccc gcctcctcgg cgaggtcggc gaggcggagg cgatcttcat
      601 ccggaatcgg acacggatcg acgaagagct gatcgcagcc gcgacaaggc tgcgcgtcgt
      661 cggccggttg ggagtcggcc tcgacaacat cgacatggaa gcctgcgccc gggtggggat
      721 cgaggtgatc ccggcggtgg gggccaacgc ggtggcggta gccgagtacg tgatgggagc
      781 gatgctcgtc ctgacccgcg gggtgttcga catgacccca tcgatggtcg ccggggagtg
      841 gccgcgacag ggtcatgctt tcgggcacga actcatgggc atgaccctcg gtctcatcgg
      901 attcggggcg atcgccagag aggtggcgaa ccgggccacc gcattcggga tgagaatcgt
      961 tgccttcgac cccttcgtcc ccgaatcaga cccggtgtgg cggaccgtgg ctcgactttc
     1021 cttcgaggaa gtgctggccg agtccgatgt ggtcagtctt cacgccccgc tcaccgaagc
     1081 cacccggaat ctgatcggtg agagcgccct ggccctgatg aagccgaatg cggtgctgat
     1141 caacacctcg cgcgggggga ccctggacga gacggctgtg gccgacgccc tgcgccgcgg
     1201 ggtcatctcg ggagccgcca tcgacgtgtt cgaatccgag ccgttgggcc cggagccagc
     1261 ctccacgttc gccgctgtgc ccaacttgct gctcacgcct cacgtcgccg ggaacaccca
     1321 cgaggccgtg gaccgggtgg ctcggatgat cgtctccagg gtgctcgaag ccatccagcg
     1381 gtgagcagcg gtcccccgcg aaatagggga acggcctcag atcagcaatc gctcctcgta
     1441 ctcgacccgg gacaggagat ggggatcacc ttcggtgatg agcaccatgt cctcgagtcg
     1501 caccccgccg cctccgcgca cgttgggcac ccacaccaaa ggctcgaccg cgaacaccat
     1561 gttcggctgg aactcgtagg tggtggcgcc gggaagggtc tccccgatgt acggcggctc
     1621 gttggcgccc atcccgatgc catggccgat gaacaggctg aagagatggt cgtcgagacc
     1681 atgatcggcg atcttggcga tgatggcgtc ggcgcagtcc tggttggtgt tccccggccg
     1741 catctgctcc agaccggcca tcagcccctc gtagaccgcg gtgtagatct tctgctgctg
     1801 tttcgaaggt ctgcccacga tcgtggtgcg gccgatatcg gcgaagtagc cattccacat
     1861 cgccccgatg tcgatgaagc acagatcctg gttgcggatg atcttgtccg tggccagacg
     1921 atggggcggg gacatgtgct ccccggaggc gacgaatggc gtgatcacgt gagccatctc
     1981 cccgccgagt tggaacagtg tctgcatcgc atcggctgcg atatccagct cacgtcgccc
     2041 ggccctggtc tcatcgaggg cccgctgcgt gaccgagtcg ccgatggcac acgcctcctc
     2101 gatcaccgcc acctcgagat cggtcttgat ccaacgggct tgctgcatca ccgtgtcgcc
     2161 gtcgatcagc tccagatcgg ggaaatgggc ccgcatcgac tggatgaacg agatgttggc
     2221 ctggtccacg gcgagtcttc ccgaggtcag ggcccgggtc tcgagcagcg gggtgaggat
     2281 gttcttgacg aatccgtcga ccaactccct ctgctccatg atggggaccg cgtgcgcctc
     2341 cccgatccaa ctcatcccga aacgcgcctt gtcgatctcg cctcctgagc cgagaaggac
     2401 ggggtcggcg tccgcggtga gcagcgcgcc gttcagcgag gtcgtcttgc cggcgatgag
     2461 ctgagcccgg agcgaggtca gatacctgac gttctcgtcc ttccacacca ggatggcgtc
     2521 tgcgtccgaa gcgcgtaacg cggtctgtgc cttgtccacg cgctgtcgac ggagctctcc
     2581 gaagtcgtat cggtcctccc agtcgacggc gaatgggccc ttggcgtatc cggtgatgtc
     2641 ggctctccct actcccggtg acctcaaatg gtgcaccgct ttactctcta tagtcggtat
     2701 agtacagaac gcatttccgt ttaccaatag tgctcgtcga tcacgccgag gagtccgcca
     2761 tgacttccca agacagtctc cgtctcgacg caaccacctc gcgccggatc accgccgtcg
     2821 acctggttcg agacgccctg cgcgccgcga tccttcgtgg agacctcccg ggaggctcga
     2881 gactggtcca gaccgagatc gcggcgcagc tcaaggtgag caccacgccg gtccgtgagg
     2941 cgatgcgaga tctctccagc gagggcctga tcaccctcga cagtcaccgg atcggcaccg
     3001 tccgcaaacc ggattgggac gagatggtgg agatcgtcga gattcgc
//
LOCUS       DATITW010000685         1508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_34010, whole genome shotgun sequence.
ACCESSION   DATITW010000685 DATITW010000000
VERSION     DATITW010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34010"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VLS86_09595"
     CDS             <1..835
                     /locus_tag="VLS86_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HSO50790.1"
                     /translation="EAGEDITLAVADPTWRIVGGWWPSHQVGAVLGAFPKTVAVVGSD
                     ARPNEDREQTRADSIHFVVLAGDGAAAVVGLPRDSWVPIAGAGTSKVNAALANGGPET
                     MMATFAELTGAEFDGYLLTGFAGFQGMIEVLGGLEIEVPRALNDRSAKASIEAGIQLL
                     SASDALAFTRVRKALPDGDFGRQANGGLALIAAATMVKTLGPVAIPGLMEQTDDLYST
                     DMSVEGLLTLAVAITQVDPALVANVVAAGSPGMAGSASVVFLRDSAYDTFADMLDGHI
                     E"
     gene            complement(900..1016)
                     /locus_tag="VLS86_09600"
                     /pseudo
     CDS             complement(900..1016)
                     /locus_tag="VLS86_09600"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
     gene            1136..>1508
                     /locus_tag="VLS86_09605"
     CDS             1136..>1508
                     /locus_tag="VLS86_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50791.1"
                     /translation="MPSDSTMRRWRSRYFTQVQAETDALSAAYMASTQTRQANRKVAI
                     VLVTAALVLTCINYAATPYWLISALEGVGWDRLATTAEDLFLGPHQGFNKLMFWGAVQ
                     VLSYVAIPLLVITLVLRERPAD"
BASE COUNT          239 a          501 c          507 g          261 t
ORIGIN      
        1 cgaggcggga gaggacatca cgctggcggt ggccgacccg acctggcgga tcgtcggcgg
       61 ctggtggccc tcccatcagg tcggggcggt gctcggtgcg ttcccgaaga cggtggcggt
      121 ggtcggctcc gacgcccgcc ccaacgagga ccgtgagcag acgagggccg actcgatcca
      181 cttcgtggtc ctggccggcg acggtgccgc ggcggtggtc gggctgcctc gagactcctg
      241 ggtgccgatc gccggcgccg gaaccagcaa ggtgaacgcc gcgctggcca atggcgggcc
      301 ggagaccatg atggcaacct tcgccgagtt gaccggcgcc gagttcgatg gctatctcct
      361 gacggggttc gccggattcc agggcatgat cgaggtcctg ggcggtctcg agatcgaagt
      421 gccccgggca ctcaacgacc ggtcggccaa ggcctcgatc gaagcgggga tccagctgct
      481 gagcgcctcc gacgccctcg ccttcaccag ggtccgtaag gctctccccg atggggactt
      541 cggccgtcag gccaacggtg gcctggcctt gatcgcggcc gcgaccatgg tcaagacact
      601 ggggcctgtc gccatccccg gtctcatgga gcagaccgac gacctgtact cgacggacat
      661 gtccgtcgag ggcctgctga ccctggccgt cgctatcaca caggtcgacc ccgcgctggt
      721 ggccaacgtc gtggcagcag gctcaccggg aatggctgga agcgccagcg tcgtgttcct
      781 ccgggacagc gcctacgaca cgttcgccga catgctcgac ggtcacatcg agtgaccgcg
      841 gcccgaccgg cgaacggttc gaaactcagg tgaagcctgc cccctccgga tagctccggc
      901 acctggatct cgaggacgag cagggtgatg acgatggcga acacaccgtc ggtgagcgcc
      961 atgacgcggg tctggtcgca ccccgcctcg tacacgtctg ggttgacgac tcccatgaga
     1021 acccccgtcc ttgtctggac gcaccgtctg gaaccgatgt tcctggggtg tcagcgatcg
     1081 attatcgcga tttggtgaga cagacggggg ggagcctgtg gctaccctcg gcatgatgcc
     1141 ctccgactcc accatgcggc gttggcggag tcgctacttc acccaggtgc aggcggagac
     1201 cgatgctctt tccgctgcct acatggcctc cactcagacc cggcaggcca accggaaggt
     1261 cgccatcgtc ctggtcaccg ccgccctcgt cctcacctgc atcaattacg cggcaacgcc
     1321 gtattggctg atctcggcgc tggaaggggt gggctgggat cgtctcgcca ccacggccga
     1381 ggacttgttt ctcgggccgc accaggggtt caacaagctc atgttctggg gtgcggtcca
     1441 ggtcctcagc tatgtggcga tcccactgct ggtgatcacc ttggtgctca gggagcgccc
     1501 ggccgatt
//
LOCUS       DATITW010000686         1616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_274131, whole genome shotgun sequence.
ACCESSION   DATITW010000686 DATITW010000000
VERSION     DATITW010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274131"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VLS86_09610"
     CDS             <1..651
                     /locus_tag="VLS86_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSO50792.1"
                     /translation="DLAGLAKVIAGDEHVLFLVDSVTGICGTEVRTDDWKLDWVLTGS
                     QKAMALPPGLSFGVASDALLERSAGLAGKGVYFDLVSLNRSLEALQTPATPAISLMYA
                     LRLQLERILEEGIETRWKRHTAMRERTLEWVEDRSGLGVAPFAPEGYRSPTVTCVSLP
                     EGVTGPAVVAGMKERDWVIGSGYGKLKETTIRIGHMGDRTVDELNLLLDDLEHVIR"
     gene            648..1607
                     /locus_tag="VLS86_09615"
     CDS             648..1607
                     /locus_tag="VLS86_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HSO50793.1"
                     /translation="MSGPYRIVVTDHVSESGLAPLLEDGRFQVTRIDDSGTDAFQAAL
                     ADADGLVVRSATRVTRDMLEKGPRLRVVGRAGVGVDNIDLPAATERGIAVLNSPAGNT
                     VAAAELTMALILSVVRRVAEADRSMRGGEWARSRLRGVQLKDRTLGLVGAGRIGSEVA
                     NRCRAFGMKILAYDPYLTIERADDLGLSLTNLDEVLTHGDIISLHVPLPDETRHLIDG
                     KAMARMKQGAFVVNVSRGGVIDEDALAEALTRGHLAGAALDVFESEPLAADSPLLELP
                     NVVLTPHLGASTVEAQELVAREIAESVRDALVLGDLSGSVNSS"
BASE COUNT          267 a          494 c          562 g          293 t
ORIGIN      
        1 gacctggcag gcctggccaa ggtgatcgct ggtgacgagc atgtcttgtt cctggtggac
       61 tcggtgaccg gcatctgcgg cacagaggtc cgcaccgacg attggaagct cgattgggtg
      121 ctcacgggct cgcagaaggc gatggcactc cccccagggc tgtcgtttgg ggttgcctcg
      181 gatgcccttc tggagcgttc ggccggtctt gccgggaagg gcgtctactt cgacttggtg
      241 tccctgaacc gcagcctgga ggctttgcag acccctgcga ccccggcgat ctcgctgatg
      301 tacgccttgc ggctgcagtt ggagaggatc ctcgaagagg gcatcgagac gcgttggaaa
      361 cggcatacgg cgatgcgaga acgcacgctg gagtgggtcg aagataggtc ggggctgggt
      421 gtagcaccct tcgcccccga ggggtaccgc tccccgaccg tgacgtgtgt cagcctcccg
      481 gagggggtga ctggccctgc cgtggtggcc ggcatgaagg aacgggattg ggtcatcgga
      541 tcgggttacg gaaagctgaa agaaaccacg atccgtatcg gacacatggg cgatcgaacg
      601 gtcgacgagt tgaacctcct tctcgacgac ctggaacacg tcattcgatg agtggcccgt
      661 accggatcgt cgtcaccgat cacgtttccg aatcgggtct cgccccgctt ctggaagacg
      721 ggagattcca ggtgacccgg atcgacgact cgggtaccga tgccttccaa gcggcgcttg
      781 ccgatgcgga cggcttggtc gtccgcagcg ccaccagggt gacccgcgac atgctggaaa
      841 aaggaccccg actgagggtc gtcggccggg ccggggtcgg ggtcgacaac atcgatctcc
      901 cggctgcgac cgagcgggga attgcggtcc tcaactcccc ggcgggaaac accgtggctg
      961 cggccgagct caccatggcc ctgatcctgt cagtcgtccg ccgggtggcc gaggctgatc
     1021 gctccatgcg cgggggcgag tgggcgcgat cacggctgcg gggggtccag ctcaaggacc
     1081 gcaccctggg cctggtggga gcgggccgga tcgggagcga ggtcgccaac cggtgtcggg
     1141 cattcgggat gaagatcctc gcctacgacc cctacttgac catcgagcgc gccgacgacc
     1201 tcgggctttc cttgaccaat ctggatgagg tcctgaccca cggcgacatc atctccctcc
     1261 acgttccgct ccccgatgag acgcggcatc tgatcgacgg caaggccatg gcgcggatga
     1321 agcaaggtgc gttcgtggtc aatgtctcca gagggggagt gatcgatgag gatgcgctcg
     1381 ccgaggcgct cacccggggt catctggcgg gtgccgcgct tgacgtgttc gaatccgagc
     1441 cgctcgctgc ggacagcccg ttactcgagc tgccaaatgt ggtgctgacg ccgcatctcg
     1501 gggcgtccac cgttgaggcg caggaactgg tcgcccgcga gatcgcagag tcggtccgcg
     1561 atgcgctggt cttgggcgac ctctccggtt ctgtcaactc gtcctaggcg cgtccg
//
LOCUS       DATITW010000687         1928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_193847, whole genome shotgun sequence.
ACCESSION   DATITW010000687 DATITW010000000
VERSION     DATITW010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193847"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VLS86_09620"
     CDS             <1..1001
                     /locus_tag="VLS86_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSO50794.1"
                     /translation="EGRPMFIDIGSFEAYRPGEPWIGYRQFTRQFLFPLLLRAWVGVP
                     FQPWLRGDIEGPTPAQMARLLGNKKRFSTGALFHVRMQARMEARMSGRAVREDLRSAG
                     FSSELILANVKKLRALVTSLEWEHEADGWSEYGSCTHVGRDRDTKSEFLGSAIERLRP
                     ARVLDLGANDGHFSMIAAKAGAETIAVDSDEAVLDDLYQRSRGLSLGIALSDLANPSP
                     AQGWAGTERSALTARARPDLVIAYGLIHHLIYAASIPPRAVVAWLRSFDCPVIVEFVS
                     PTDEMVAKLVGNKLPHELHAEREEGQFRTLLGEMFDTAEEMVLASGTRALFSLRPI"
     gene            998..>1928
                     /locus_tag="VLS86_09625"
     CDS             998..>1928
                     /locus_tag="VLS86_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50795.1"
                     /translation="MTLVRLGTTPWWKVPLSLGAVGAFALAQPLLDLLGRNPEFFIAR
                     GFTPLDVLLLPLALLTLPATLAVPVLALRLVGPRTSAIAHLIALGALFALLVASVWVA
                     LLGSETPPGVFGLVTVLSGAAFAVAYLRFPTLQSAVGYASWALLAFPAWFLIMTPASD
                     VAFASAGDLPETGEISNPGPIVMLVFDEFPLATIIDSDGELLDELFPNFAQLAEDGIW
                     YRNGVGVRQQTEEALPTILTGIGADPGSIPIASDHPLNLFTLLSDAYDIAAVETVTDL
                     CPDFACTNSSRRIEPFATRWSALTVDLAVVYGHL"
BASE COUNT          290 a          663 c          617 g          358 t
ORIGIN      
        1 tcgagggccg accgatgttc atcgacatcg ggtcgttcga ggcctaccgg ccgggtgagc
       61 cctggatcgg ctatcgccag ttcacccgtc aattcctctt cccgttgctg ctccgggcct
      121 gggtcggcgt tccgttccaa ccctggcttc gaggagacat cgaaggcccg accccggcgc
      181 agatggcgcg tcttctcggc aacaagaagc ggttcagcac gggcgccctg tttcacgtcc
      241 gaatgcaggc ccggatggaa gcgcggatgt cgggccgggc ggtcagggag gacttgcgca
      301 gcgcaggctt cagctcggag ctcatcctgg ccaacgtcaa gaagctccgc gctctggtga
      361 cgtccctcga atgggagcac gaagccgatg gttggtccga gtacggctcc tgcacccacg
      421 tcggacggga tcgcgacacc aagagcgagt tcctcgggtc ggcgatcgag cgcttgcgcc
      481 cggcccgggt cctcgacctc ggagcgaacg acggtcactt ctcgatgatc gcggccaaag
      541 ccggggcgga gacgatcgct gtcgacagcg acgaggcggt cctcgacgac ctctaccaac
      601 ggagcagagg gctgagtctg gggatcgcgc tcagcgacct ggccaacccg tctcccgctc
      661 agggatgggc gggaaccgag aggtcggcgc tgacggcccg ggctcgtccc gacctggtga
      721 tcgcctacgg tctgattcac cacctgatct acgccgcgag catcccgccc cgggcggtgg
      781 tcgcctggct gcggagcttt gactgcccgg tgatcgtcga gttcgtctct cccaccgacg
      841 agatggtggc caagctcgtc ggcaacaagt tgccccacga gctccacgcc gagcgggagg
      901 agggccagtt ccgcacgctt ctgggagaga tgttcgacac cgccgaggag atggtgctcg
      961 catcgggcac ccgggctctc ttttccctgc ggcccatatg acgctcgtcc gactcgggac
     1021 gaccccgtgg tggaaagtgc ctttgtcgct cggcgccgtc ggagccttcg cccttgccca
     1081 gcctctcctc gatctcctcg ggcgcaatcc cgagttcttc atcgcacggg ggttcacccc
     1141 cctcgatgtc ctgctcttgc ccctcgctct cctgacgctg cccgcaaccc tcgccgtgcc
     1201 ggtgctggcc ctgcgattgg tagggccacg aacgtcggcg atcgcgcatc tgattgctct
     1261 cggcgcactc ttcgcgttgc tggtggcctc ggtctgggtg gcacttctcg gaagtgagac
     1321 gccaccgggg gtcttcgggc tggtcactgt cctgagcggc gccgcgttcg ctgtcgccta
     1381 cctccgattc cccaccctgc agagcgcggt cgggtacgca tcttgggccc tgctggcctt
     1441 ccccgcctgg ttcctgatca tgactccggc gagcgacgtc gcgttcgcca gcgccgggga
     1501 ccttcccgaa accggcgaga tctccaatcc ggggccgatc gtgatgctgg tgttcgacga
     1561 gttcccactg gcgacgatca tcgacagcga cggcgaactg ctcgacgagc tcttcccgaa
     1621 cttcgcccag ctcgccgagg acggcatctg gtatcgaaac ggggtggggg tacgccaaca
     1681 aaccgaggag gcgcttccca ccatcctgac cgggatcggg gccgacccgg gaagcatccc
     1741 gatcgcctcg gaccatccgc tcaacctctt cacactgttg tcggatgcgt acgacatcgc
     1801 agctgtggag acggtcacgg acctgtgccc ggacttcgcc tgtaccaact cgagtcgccg
     1861 gatcgagccg ttcgcaaccc gttggagcgc cctcaccgtt gatctggcgg tggtgtacgg
     1921 gcatctcg
//
LOCUS       DATITW010000688         1745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_394259, whole genome shotgun sequence.
ACCESSION   DATITW010000688 DATITW010000000
VERSION     DATITW010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394259"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..>1745
                     /locus_tag="VLS86_09630"
     CDS             146..>1745
                     /locus_tag="VLS86_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSO50796.1"
                     /translation="MLQTLVAPGRACARRNGVIKLNPSHEAIQRSLEHLIREDSPDLH
                     TVTTVDGVFRFVSPASFPLFGWEPDYLVGRHQDGFIHPDDAALVDQARHRASSNPPTA
                     VTTVYRFRCADGAYRWTEAVATVVDAEGENLVVSAVRDIGDRKKSELDLERQATTDVL
                     TGTANRAVFMDRLQQALRRLERRPGVVAVLFLDLDRFKLINDSVGHLIGDAVLLKMAQ
                     RLRRFMRPQDPLARWGGDEFAIVIEGISSSVEAETLGARIIEAGRVPFVVGDERFICT
                     TSVGIAVTTDPHHSAEGLLQESDMALYRAKDRGRDRAEMFDEDLRTQAVGRLGTERML
                     RRAIDEDRLRVSYQPIVDLKTGDTVAAEALVRVWDPEQSGLIAAEAFIGIAEETGLLA
                     GIDDWVLGQTIEQAKIWRDLFAGTGFSDIAINLTARHLADAGFAQSVLDSLSAHLLPT
                     ASLQIEVTERVLMEASNSAMTGLKLLRDAGVKVGLDDFGTGYSSLSYLRQFPLDFVKI
                     DQSFIHDLVRTEGDRAIVAATIGLC"
BASE COUNT          312 a          574 c          547 g          312 t
ORIGIN      
        1 cctgccgtcc tcggcaccaa cctgcccaga ccggatcccg gtgacgacgg gtcttggtgg
       61 tggcctatcc cgggactagt gttatccaca gcagacagtt gccgcgaggt ccgtcgcact
      121 cgagggacca cacgggcttt acgcgttgct ccagaccctg gtagcgcccg gcagggcatg
      181 tgccaggagg aacggcgtca tcaaactcaa cccgtcccat gaggcgatcc agcgatccct
      241 ggagcatctg atccgggaag actcccccga tctccacacg gtcaccacgg tcgatggggt
      301 gttcagattt gtctccccgg cgagctttcc cttgtttggc tgggagcccg actatctggt
      361 gggacgacac caggacggtt tcatccatcc cgacgatgcg gctctcgtcg accaagctcg
      421 tcatcgcgcc tcgagcaacc ctccgactgc ggtcaccacg gtgtatcgat tccgatgcgc
      481 cgatggcgcc taccgatgga cggaggcagt cgccaccgtg gtcgacgccg agggtgagaa
      541 cctggtcgtt tctgccgtgc gtgacatcgg ggaccgcaag aaatccgagc tcgacctcga
      601 gcgtcaggcg acgacagacg tgcttaccgg caccgcgaat cgggcggtgt tcatggatcg
      661 gctgcaacag gcactgcgcc ggctggaacg ccgaccgggc gtcgtagccg tcctcttcct
      721 ggatctcgac cggttcaagc tcatcaacga ctccgtgggg catctcatcg gcgacgcggt
      781 cctactcaag atggcccagc gtctgcgcag gttcatgcga cctcaggacc cgttggcgcg
      841 ctggggtgga gatgagtttg caatcgtgat cgagggaatc tcgtcctcgg tggaagcgga
      901 gacacttggc gcccgaatca tcgaggccgg ccgagtcccg tttgtggtgg gtgacgagcg
      961 attcatctgc acgaccagcg tggggatcgc agtgacaacg gatccccacc acagcgccga
     1021 ggggctgctg caggagtccg acatggcgct gtaccgggcc aaggaccggg gccgtgaccg
     1081 ggccgagatg ttcgacgagg accttcgcac ccaggcggtc gggcgcctcg gcaccgagag
     1141 aatgctgcgc agggccatcg acgaggacag actccgggtc agctaccaac ccatcgtcga
     1201 tctcaagacc ggggacacgg tcgcagccga ggcattggtc cgggtgtggg accccgagca
     1261 gtccggtctc atcgccgccg aggctttcat cgggatcgcc gaagagacag ggctcctggc
     1321 cggaatcgac gactgggttc tgggacagac catcgagcaa gccaagatct ggcgcgactt
     1381 gttcgcgggc accggcttct ccgacatcgc catcaacctg accgcgcgtc acctggccga
     1441 cgccgggttt gcccaatcgg tgctcgacag cctcagcgcc cacctcctcc cgacggcctc
     1501 actgcagatc gaggtcactg aacgagtcct gatggaggcc tccaactcgg cgatgaccgg
     1561 tctgaagctg ctccgggatg cgggagttaa ggtcggtctc gacgactttg gcaccggcta
     1621 ttcctcgctg tcctacctcc gacagttccc actcgacttc gtcaagatcg accagtcctt
     1681 catccacgac cttgttcgca ccgagggcga ccgagccatc gtcgccgcga cgatcggcct
     1741 ttgtc
//
LOCUS       DATITW010000689         4595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_635232, whole genome shotgun sequence.
ACCESSION   DATITW010000689 DATITW010000000
VERSION     DATITW010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635232"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VLS86_09635"
     CDS             <1..1001
                     /locus_tag="VLS86_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016909860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HSO50797.1"
                     /translation="LLVAACGGSEGGQPDSLTLIAHESFAGGVTEGTFAAFIEDTGIE
                     VEVIAAGDAGSMVNQAALTKDNPLADVLFGVDDTFLSRALDEEIFSVYRSPLLDKVGI
                     EADPSDRVTPIDFGDVCINYDKSWFDETGTPIPSTLDELRDPTYAGVLTVEHPATSSP
                     GLAFMLATIDEFGEEGWLEFWADLEEGRVGVAPDWDTAYYSDFRPYGGESSMVVSYAS
                     SPPAEVIFATEPLTEAPTGVIEAGCFRQVEYAGILAGTEFPEAAGELIDFMLSTGFQE
                     TIPLTWFVFPVNEDAALPQEFIDHTIVPSDPARLDAATIAQNRDRWIGEWVELMEG"
     gene            1002..2552
                     /locus_tag="VLS86_09640"
     CDS             1002..2552
                     /locus_tag="VLS86_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSO50798.1"
                     /translation="MKRIRWGQVLVAGIPALFITYFFLYPVARIIILGLSELSVGTDS
                     VEVRLLRVGWFTLWQATVSTILTLLVAAPMTWAVSHFEFRGRRLATALVTVPFVLPTV
                     VVGAAFVSLGWRDSIGAILAAHVFFNIAVVVRTVGALWSRLDPDLHDAGRVLGAGEWA
                     VFRTITLPLLRPAIAAAASIVFLFAFTSFGVVLILGGYQYATLEVEIYRQAVALFDLP
                     LAAALAVIQLIGVTATLYAYSRYQERHTSTWALRSDAARPRPQGRTRLLVAASVGVTL
                     LALSLPIAVLVARSLQPGSYRALVEQDPVVGVPLSSAGNSLLFAVIASTIAVSIGVMA
                     SAVISGRTGRLSRWFDLTLMLPLGTSAVTIGFGFIVALGWPVDLRASFWLVPIAHALV
                     AIPFVVRATVPTLRSIPGETREAAKVLGASPGRVWREIDLPIVSRAALVGAAFAFVIS
                     LGEFGATSFVARPSTATMPIMIFRLLSRPGAGSLAMARALAVVLAGLTAAVVLWIDRL
                     QFGEIGTF"
     gene            2552..3253
                     /locus_tag="VLS86_09645"
     CDS             2552..3253
                     /locus_tag="VLS86_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50799.1"
                     /translation="MLRVEHLEVRFGDIEVLHDVNLAVGDGESVAVLGPSGSGKSTLL
                     RAVAGLVPLASGAITWDGADVTAVPTHKRGFGLMFQGYALFPHMDVAGNVGFGLKMAG
                     IAPGEIRDRVDTALDWVGLAGFGERAIVDLSGGEKQRVALARTLAPEPVLVMLDEPLG
                     ALDRALRERLMTETRQILTERGVTTLVVTHDREEADALSDRLALMRDGTIVQSGSLED
                     LLDHPADDWVRDFLS"
     gene            complement(3250..>4595)
                     /locus_tag="VLS86_09650"
     CDS             complement(3250..>4595)
                     /locus_tag="VLS86_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSO50800.1"
                     /translation="AHPPTRLVSRPALPGEHMSGLGVQLTLILVLLLVNGLLAGSEIA
                     LISLREPQIARLEAGSRTGRRLAALARDPNRFLATIQIGITLSGFLASATAAVSLAEP
                     LIPVFGFAGDAARALAIVSITLVLSFVTLVVGELAPKRLALQQAERWAMLVANPLHVG
                     AIIARPVVWLLSRSTDFVVRLLGGEPGQTRETVEFEELRDVVLAHRGLSEAHQEVLVG
                     AFEVAERTVRQVLVPRSDVVVIGSDMTAGQALATLLDSAHSRAPVAPGRDLDQTIGIA
                     HIRDLVAAGPSDPVSSVAIEPVAVPESVPVLAALRQLQTERQQMALVVDEHGGVEGII
                     TVEDLVEELVGEIYDESDPDLVAVRHNADGSMVVAGSFPLHDLVDLEVEVPPADQTTV
                     AGLVLSHLAQVPEAPGDTVVIADWKFTVLSVSGRKITEVELRRAEPEPTSRGARP"
BASE COUNT          697 a         1564 c         1509 g          825 t
ORIGIN      
        1 tgctcttggt cgccgcctgt gggggatccg agggcggcca accggatagc ctcaccctca
       61 tcgcgcatga gtccttcgcc ggtggtgtga ccgaaggcac cttcgccgcg ttcatcgagg
      121 acaccgggat cgaggtcgag gtgatcgcgg cgggtgatgc cggatcgatg gtcaatcagg
      181 cagccctgac caaggacaac ccgctggccg acgtcctgtt cggtgtcgac gacacttttc
      241 tctctcgggc cctcgacgag gagatcttct cggtgtatcg cagcccgctt ctggacaagg
      301 tcgggatcga agccgacccc agcgaccggg tgacacccat cgacttcggg gacgtgtgca
      361 tcaactacga caagtcgtgg ttcgacgaaa ccgggacacc gatcccgagc acgctcgacg
      421 agttgcgaga tccgacttat gccggagtgc tgaccgtcga gcacccggcc acatcctctc
      481 cgggtctggc gttcatgctg gccacgatcg acgagttcgg cgaggagggc tggctcgagt
      541 tctgggccga cctcgaggaa ggcagggttg gcgtcgcccc cgactgggac accgcctatt
      601 acagcgactt ccggccctac ggtggggaga gctcgatggt ggtgtcctac gcctcatctc
      661 ccccggccga ggtgatcttc gccaccgagc ccctgaccga agccccgacc ggagtcatcg
      721 aagccgggtg cttccgccag gtcgaatacg cagggatcct cgccgggacc gaattccccg
      781 aggcagcagg cgaactgatc gacttcatgc tgtccaccgg gttccaggag acgatccccc
      841 tcacctggtt cgtctttccg gtcaacgagg acgccgctct ccctcaggag ttcatcgacc
      901 acaccatcgt cccctccgac ccggcccgtc tcgacgccgc caccatcgcc cagaaccgcg
      961 accggtggat cggtgagtgg gtcgagctga tggagggctg attgaagagg attcgctggg
     1021 gccaggtgct ggttgccggg atcccggcgc tgttcatcac ctacttcttc ctctatccgg
     1081 tggcccggat catcattctc gggctgtcgg agttgagcgt cggcaccgac agcgtcgagg
     1141 ttcgactgct cagggtcggt tggttcacac tgtggcaggc gacggtctcg accattctca
     1201 ccctgctcgt cgccgctccg atgacctggg cggtgtccca cttcgagttt cggggccgcc
     1261 ggctggcaac ggccctggtg accgtgccct tcgtcctccc cacggtcgtg gtcggtgcgg
     1321 cgttcgtgtc gttgggatgg cgagactcga tcggagccat cctcgccgca catgtcttct
     1381 tcaacattgc ggtggtggtt cgcacggtcg gcgcactgtg gtcccgtctc gaccctgacc
     1441 tccacgatgc gggccgggtg ctcggggcgg gtgagtgggc agtgttcagg accatcaccc
     1501 ttcctttgct ccgccccgcg atcgctgccg cggcttcgat cgtcttcctg ttcgccttca
     1561 cctcgttcgg cgtggtcctc atcctcggtg gctatcagta cgcgacgctc gaggtggaga
     1621 tctaccgcca ggcggtggcg ctcttcgacc tgcccctggc cgcagccctc gccgtgatcc
     1681 agttgatcgg ggtcacggcc accctctacg cctactcgcg ataccaggag cggcacacct
     1741 cgacgtgggc gttgcgctcg gacgctgcga gaccccggcc acagggtcgc acccggctcc
     1801 tggtcgcagc aagtgtcggc gtcaccctgt tggctctgtc cttgccgatc gccgtcctgg
     1861 tcgcccgatc gctgcagccc ggctcatatc gggccctggt cgaacaggac ccggtggtcg
     1921 gcgtgcccct ctcctcggcc gggaacagcc tgctgttcgc cgtcatcgcc tcgaccatcg
     1981 cggtctcgat cggggtcatg gcgtcagcag tgatcagcgg ccgcaccggc cgcctctcgc
     2041 gctggttcga cctgacgctg atgctccccc tgggcacctc ggcggtcacc atcggattcg
     2101 gtttcatcgt ggccctcggg tggcctgtcg acctcagggc ctcgttctgg ttggtcccga
     2161 tcgcccacgc cctggtcgcg atcccgttcg tcgtcagggc aaccgtcccc acgctccgct
     2221 cgatacccgg cgagacccgg gaggcggcga aggtgctcgg ggcgtctccg ggacgggtgt
     2281 ggcgtgagat agacctgccc atcgtcagcc gcgccgcctt ggtgggggcg gccttcgcct
     2341 tcgtgatctc cttgggtgag tttggcgcta catcgtttgt cgccaggccg agcacggcca
     2401 ccatgccgat catgatcttc cgtctcctct ccagacccgg cgccggttcc ctggccatgg
     2461 ccagggcgct ggccgtggtc ctggctggcc tgaccgcggc ggttgtcctc tggatcgatc
     2521 gtctccagtt cggcgagatc gggaccttct gatgctccgg gtcgagcatc tcgaggtccg
     2581 attcggagac atcgaggtgc tgcacgatgt gaatctcgcg gtgggggacg gggagtcggt
     2641 cgcggtcctc ggcccctccg ggtccggcaa gtcgaccctg cttcgagccg tcgccggtct
     2701 ggttccgctg gcctcggggg cgatcacctg ggacggagcc gacgtcaccg ccgtgccaac
     2761 ccacaaacgg ggttttggcc tcatgttcca gggctatgcc ctcttcccgc acatggacgt
     2821 ggcggggaac gtcggctttg gcctgaagat ggccggcatc gccccgggcg agatcaggga
     2881 ccgggtcgac accgcgctcg actgggtcgg tctcgccggt tttggcgaac gcgccatcgt
     2941 cgatctctcc gggggcgaga agcaaagggt tgccctcgcc cgcaccctcg cacccgaacc
     3001 ggtgctcgtg atgctggacg agccgctggg ggcgctggac cgggcgctac gggagcggct
     3061 gatgaccgag acccgtcaga tactgaccga gcgaggcgtc accacactgg tggtgacaca
     3121 cgaccgtgag gaggccgacg ccctctccga ccgtctggcg ctgatgaggg acggcaccat
     3181 tgtccaatcg ggctcactgg aagatctcct cgaccacccc gccgacgact gggtgaggga
     3241 ctttctctcc tagggccgtg cgccccggga tgtcggttca ggttcggccc tccgcagctc
     3301 gacctctgtg atcttgcggc cggaaacgga gagaaccgtg aacttccaat cggcgatcac
     3361 cacggtgtcg ccgggcgctt cgggcacctg ggccagatgg ctcaggacca gcccggctac
     3421 cgtcgtctgg tccgcaggcg gcacctccac ctcgaggtcg acgaggtcgt ggaggggaaa
     3481 gctgccggcg acgaccatcg agccgtcagc gttgtggcgg actgccacca ggtcgggatc
     3541 gctctcgtcg tagatctcgc cgaccagctc ctcgacgagg tcctccacgg tgatgattcc
     3601 ttccacccct ccgtgctcgt cgacgaccag agccatctgt tggcgctcgg tctgaagctg
     3661 ccgcaaggcg gcaaggaccg ggaccgattc ggggacggcc accggttcga tcgccaccga
     3721 cgaaaccggg tcggagggac cggccgccac caggtctcga atgtgggcga tcccgatggt
     3781 ctgatcgagg tctctgcccg gagccacggg cgccctcgag tgagcgctgt cgagaagcgt
     3841 cgccagggcc tggccagcgg tcatatccga accgatgacg accacgtcgg atcggggaac
     3901 caacacctgt ctcacggtcc gctccgctac ctcgaacgcg cccaccagca cctcctggtg
     3961 ggcctccgag agcccgcgat gggccaggac cacatcgcgc agctcctcga attcgaccgt
     4021 ctccctggtt tgaccgggct cgccaccgag gagacggacg acgaagtcgg tggatcggct
     4081 cagcagccac accacggggc gggcgatgat ggccccgacg tgcagtgggt tggcgaccaa
     4141 catcgcccac cgctccgcct gctggagcgc aagacgtttc ggggcgagct cccctaccac
     4201 cagcgtgaca aatgacagca cgagcgtgat cgacacgatc gccagcgccc gggccgcgtc
     4261 gcccgcaaaa ccgaacaccg ggatcagtgg ttcggcaagc gagaccgcag cggtcgccga
     4321 agcgagaaag ccggacaggg tgatcccgat ctggatggtg gccaggaacc ggttcgggtc
     4381 gcgggccaat gcggcaagcc ggcgaccggt cctggacccg gcttcgagcc gggctatctg
     4441 cggctcgcgc agcgagatga gcgcgatctc gcttcccgcc aggagaccat tgaccaacag
     4501 gagaaccaga atcagtgtga gctggacgcc caggccactc atgtgctcac cgggcagcgc
     4561 gggccgcgag acgagacggg tcggcggatg ggccg
//
LOCUS       DATITW010000690         1600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_674508, whole genome shotgun sequence.
ACCESSION   DATITW010000690 DATITW010000000
VERSION     DATITW010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674508"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..>1600)
                     /locus_tag="VLS86_09655"
     CDS             complement(71..>1600)
                     /locus_tag="VLS86_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HSO50801.1"
                     /translation="YGDEYLSPKPRVYASKVKGAQEAHEAIRPAGEAFRTPEQVGAEV
                     GPDEARLYDLIWKRTLASQMADAVGVTVSAKLGATTPDGVKAVFSTSGRTITFPGFLR
                     AYVQGADDPESDLDDREEILPPLAEGDPLAAQRLEPTGHETRPPARYTEASLIQRLEE
                     LGVGRPSTYASIMSTIQDRGYVWKRGTALVPTFTAFGVVTLLELHFPNLVDYAFTAEM
                     EDDLDRIAAGDEQTLPYLTAFYFGADGLKEKVATRLDEIDPRSVGSIPIGEDGTGQPI
                     LARIGRYGPYVECGEERASIPQDVAPDELTVAKAVELLQTQNAGDKIFGTDPDTGLTV
                     YGRTGRFGPYVQLGEVEEGSKEKPKRASLLKTMSLDGLSLDDALRLLSLPRIVGTDES
                     GVEIVALNGRYGPYIQRADDRRSLVAEEHLFTLTVDEALKLLAEPPRRRGQRNAGPLK
                     ELGADPVSGKAVSVRSGRFGPYVTDGEVNASLRQGDSPETMTLERAAELLAGRRERIG
                     G"
BASE COUNT          251 a          509 c          523 g          317 t
ORIGIN      
        1 tcggcctatt gggcccggta gttgagcctc cccgtacccg atcttgtgtt cgagcgccca
       61 ctgggggcga tcaacctccg atgcgctccc ggcgaccggc gagcaattcg gccgcccgct
      121 ccaatgtcat ggtctccggt gagtcgcctt gtcgcaggga tgcgttcacc tcaccgtcgg
      181 tcacgtaggg gccaaagcgc cctgagcgca ccgacaccgc ttttccgctg accgggtcgg
      241 ctccgagctc cttgagaggg ccggcattgc gctggccgcg tcgtcgaggt ggctcggcga
      301 ggagcttgag cgcttcatcg acggtgaggg tgaagagatg ctcttcggct acgaggcttc
      361 gccggtcgtc ggctctttgg atgtacgggc cgtagcgccc gttcaaagcc acgatctcga
      421 cgcctgattc gtcggttccc acgatgcgag ggagggacag gagacgcagg gcgtcgtcga
      481 gggacagccc gtcgagtgac atcgtcttga gcaatgacgc tcgctttggc ttttccttgc
      541 tcccctcctc gacctccccg agctggacgt agggcccgaa tcgcccggtt cttccgtaca
      601 cggtgaggcc ggtgtcggga tcggtcccga agatcttgtc acctgcgttt tgggtttgga
      661 gaagctcgac tgctttggca actgtcagtt cgtcgggcgc cacatcctgg gggatggaag
      721 ccctttcctc gccacactcg acgtatggcc cataacgacc gatccgagcc aggatcggct
      781 gacctgtgcc gtcctcacct atgggaatcg agccgaccga ccgggggtcg atctcgtcga
      841 gacgggtcgc caccttctcc ttgagaccgt ccgcaccgaa gtagaaagcg gtcaggtagg
      901 gaagcgtctg ctcatcgccg gcggcgattc ggtcgaggtc gtcctccatc tcggcggtga
      961 acgcgtagtc gacgaggttg ggaaagtgga gctcgagcag ggtcaccact ccgaaggcgg
     1021 tgaacgtcgg aaccagggca gtccctcgct tccagacata ccctcggtcc tggatcgtcg
     1081 acatgatcga cgcgtacgtg gacggtcggc ccaccccgag ctcctcgagt ctctgaatca
     1141 gggaggcttc ggtgtagcgg gcaggtggtc gggtctcgtg accggtcggc tccagacgtt
     1201 gtgccgccag cggatcacct tcggccagtg gggggagaat ctcctcacgg tcgtcgaggt
     1261 cggactcggg atcatccgct ccctgcacgt aggcgcgcaa gaagccgggg aaggtgatgg
     1321 tgcgcccact tgtgctgaaa accgccttca ctccgtccgg ggtggttgcg cccagcttcg
     1381 ccgaaacggt gactccaaca gcgtccgcca tctgcgatgc cagggtccgt ttccagatca
     1441 ggtcatagag cctcgcctca tcgggaccga cctcggcgcc gacttgctcc ggcgtccgga
     1501 aggcctcacc cgcggggcga atcgcctcgt gggcctcctg cgcgcctttg accttcgagg
     1561 cgtagaccct gggcttgggc gagaggtatt cgtctccgta
//
LOCUS       DATITW010000691         1880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_635266, whole genome shotgun sequence.
ACCESSION   DATITW010000691 DATITW010000000
VERSION     DATITW010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635266"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..>1880)
                     /locus_tag="VLS86_09660"
     CDS             complement(4..>1880)
                     /locus_tag="VLS86_09660"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HSO50802.1"
                     /translation="GFSAAQDLEAADIEASRLTIDNMRIWDPSVLPRTYQNFQELRNY
                     YTLGVVDTDRYLNQGTPTQVMLAVRELEEVALPRDDWLNQRLLYTHGFGAVVNVANVV
                     ADDGQPRFLLSDVPPVAAVEDFELTEPRVYFGETYQEGRPIIVKTGDEPQEVDFPLAD
                     EGTAYNEYAGEAGVELNSIWKRLAFAFRYRDLNLFISGEVRADSRVLVERNVGHILEA
                     VAPFLHLDTDPYPVILDGKILWVVDLYTTSSWYPYSQPLENAAIGRLAQSSQLRRGLN
                     YMRNSVKAVVDSHDGHVTFYLVDPDDPLAVAWSEAFPGLFTPASEMPEGLEEHLRYPQ
                     DLFRVQGQLYLEYHVNDANELFSGNDAWSLPLDPATISRDSASLGGSELLFGDVRDPT
                     TAELRYRAEILPYYLLTKLPGEEDLSYLLLQPFTPSSRRNMSSFLVADSTPGRYGRLI
                     DFRMPQGDFVDGTEQVGQRIDQDGDISEQFTLWDNQGSTVIKGDMLVIPIEDALLYIQ
                     PIFLEAEGGGIPEFRRVIVVYGDQVEWAETLDLALAQVFGEGTGGEPVEPEPTPPGGE
                     TVAELLAEAVAAFRQADEALTAGDLAGYQRWVNEAERLIDEAQAILESAVEARAGSAG
                     "
BASE COUNT          320 a          614 c          610 g          336 t
ORIGIN      
        1 cctctagccg gcagagcctg cccgtgcctc gaccgcactc tccaggatcg cctgagcctc
       61 gtcgatcagc cgctccgcct cgttgaccca cctctgatac ccggcgagat caccggcggt
      121 gagcgcctcg tctgcctgcc ggaatgccgc aacggcctcg gccagcaact cggccaccgt
      181 ctcgccgccc ggcggcgtcg gctctggctc caccggctcg cccccggtgc cctcgccgaa
      241 cacctgcgcc agggccaggt cgagagtctc ggcccattcc acctggtcgc cgtagaccac
      301 gatgaccctg cggaactcgg ggatcccgcc cccctcggcc tcgaggaaga tcggctggat
      361 gtagaggagt gcgtcctcga tcggaatcac gagcatgtcg cccttgatca cggtggagcc
      421 ctggttgtcc cacagcgtga actgctcgga gatgtcgccg tcctgatcga tgcgctgtcc
      481 gacctgctcg gtcccatcga cgaagtcccc ttgcggcatc ctgaagtcga tcagcctccc
      541 atagcggccc ggggtggagt cggcgacgag gaagctggac atgttcctcc gactcgaggg
      601 ggtgaagggc tggagcagca gatacgacag atcctcttcg ccggggagct tggtcaggag
      661 gtagtagggc aggatctccg cccgatacct gagctcggcg gtggtcgggt cacggacgtc
      721 gccaaacagc agctccgagc cacccagcga cgcgctgtca cggctgatgg tggccgggtc
      781 gagcgggagt gaccacgcat cgttcccgct gaacaactcg ttggcgtcgt ttacgtggta
      841 ttccaggtag agctggccct gcacccggaa caggtcctgg ggatagcgga gatgctcctc
      901 gaggccttcg ggcatctctg aggccggggt gaacaacccg ggaaacgcct cggaccacgc
      961 tacggccagc gggtcgtcgg gatcgaccag gtagaaggtg acgtgcccgt cgtgggaatc
     1021 gaccacagcc ttcaccgagt tgcgcatgta gttgagcccc cggcgcagct gggaggactg
     1081 ggccagccgg ccgatggccg cattctccaa tggctgggag tagggatacc agctgctggt
     1141 ggtgtacaga tcgaccaccc ataggatctt cccatcgagg atcaccgggt aggggtcggt
     1201 gtcgaggtgg aggaatggcg ccacggcctc caggatgtgg ccgacgttcc gctccacgag
     1261 tacccggctg tcggcgcgga cctctcccga gatgaacagg ttcaggtcgc ggtatcggaa
     1321 ggcgaatgcc agccgcttcc agatgctgtt cagctcgacg cctgcctcac cggcgtactc
     1381 gttgtaggcg gtcccctcgt cggccagggg gaagtcgacc tcctgaggct cgtctccggt
     1441 cttgacgatt atcggccgac cctcctgata ggtctcgccg aaatagaccc tcggctcggt
     1501 cagctcgaag tcctcgaccg cggccaccgg cgggacgtcg ctgagcagga agcggggttg
     1561 cccgtcgtcg gcgaccacgt tggccacgtt gaccacagcg ccaaacccgt gggtatacag
     1621 gagtcgctga ttcagccagt cgtcacgggg gagcgcaacc tcctccaact cacgaacggc
     1681 gagcatgacc tgggtaggtg ttccctggtt gagatagcgg tcggtgtcga ccactcccaa
     1741 cgtgtagtag ttcctcagct cctggaagtt ctgataggtc cggggaagga ccgatggatc
     1801 ccatatcctc atgttgtcga tggtcagccg acttgcctcg atatcggcgg cctccaggtc
     1861 ctgggcagcg gagaaccccc
//
LOCUS       DATITW010000692         1828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_635325, whole genome shotgun sequence.
ACCESSION   DATITW010000692 DATITW010000000
VERSION     DATITW010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635325"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="VLS86_09665"
     CDS             <1..1183
                     /locus_tag="VLS86_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50803.1"
                     /translation="MSLAAGWISVGALVIATLIGGILGIALGIVIESILGLEVLEANG
                     AAWVAIAMLFLLWLLLVVAVLLSWRPAEHTSTQGAQVLLRRVVLNAGWLFRAAAGYGL
                     MAGGVATVVVWQAGSWDPSRLTVPEPGGTIYWVAIGMGVLLVVLAFIRVWTHFGWKPA
                     PLVLIGAALVVFAASKDFFAVEFFKVQIALKDNLVDIAVAVAIVVPGAFMLRSIWTGA
                     GSSEAGRNKRRSVGILWDIGSFWPRWFHPLAPPAYGPVAVTELRKEIQTHQRQVLAAH
                     SQGSLIAALALIHTAEGKRPQRFLTYGSQLGHLYPQMFPDVGIDGEAGLVDTVRGIYT
                     GRWINLYRETDPIGGHFVEILGGFNGVVRTGTGHSRYEPTPEFRRARAGQPATDSLTG
                     E"
     gene            complement(1227..1481)
                     /locus_tag="VLS86_09670"
     CDS             complement(1227..1481)
                     /locus_tag="VLS86_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50804.1"
                     /translation="MSPRLAVVTLWLVTDWVGDAFNDGWILPVLGIVLLPWTTALYVT
                     GFALGDGAAPWGILGAMIGLFLDVAVHAGSAPMGRRRYAS"
BASE COUNT          314 a          602 c          592 g          320 t
ORIGIN      
        1 catgtcgttg gccgccggat ggatctcggt tggggccctg gtgattgcca ctctgatcgg
       61 cgggatactc ggcatcgccc tcggcatcgt catcgaatcg atcctcggtc tcgaggtgct
      121 cgaagccaat ggggccgcct gggtggccat cgccatgctc ttcctgctct ggctgctgct
      181 ggtggtcgca gtcctcctca gctggcggcc cgccgagcac acctcaaccc agggtgccca
      241 ggtgttgttg cgtcgggtgg tcctcaatgc cggctggctg tttcgggcgg ctgcgggtta
      301 tgggctgatg gcgggcggag tggcgactgt cgtggtgtgg caagccggct cgtgggaccc
      361 gagcagactc accgtgcccg aacccggcgg caccatctac tgggtggcaa tcgggatggg
      421 cgtcttgctc gtggtcctcg ccttcatccg ggtgtggacc cacttcggct ggaagccggc
      481 gcccctcgtc ctgatcggtg cggccctggt cgtgtttgcg gcaagcaagg acttcttcgc
      541 tgtcgagttc ttcaaggtcc agatcgcact caaggacaac ctggtggaca tcgcagtggc
      601 cgtcgcgatc gtggtgcccg gcgccttcat gctgcgcagc atctggaccg gagccggaag
      661 cagcgaggcg ggccggaaca agcgaaggag cgttggaatc ctgtgggaca tcggctcgtt
      721 ctggcctcgc tggttccatc cccttgcccc gccggcgtat ggcccggtgg cggtcaccga
      781 gctgcgcaag gagatccaga cccatcagag acaggtcctg gccgcgcact cgcaagggtc
      841 tctgatcgcc gccctggccc tgatccacac cgcagagggc aaacgtcctc agcggttcct
      901 cacctacggc tcacaactgg ggcacctcta tccgcagatg ttccccgacg tgggaatcga
      961 cggcgaggcc gggctggtgg acacggtgag gggcatctac accgggaggt ggatcaatct
     1021 ctaccgggag accgacccga tcggagggca cttcgttgag atcctcggtg gcttcaacgg
     1081 ggtggtgcgc accgggacgg gtcactcccg atatgagccg acacctgagt ttcgcagagc
     1141 ccgagccggg caaccggcga ccgactccct gactggcgag tgagtcgaca ggcaggtcag
     1201 ggcaacggca tcacccgaat cggaggtcag gaggcgtagc gacgacgccc catcggcgca
     1261 gagccggcat gaaccgcaac gtctaggaag agcccgatca tcgctccgag gattccccac
     1321 ggcgcggcac cgtctccgag cgcgaatccc gtcacataca gggctgtggt ccatggcagg
     1381 agcacgatcc cgaggaccgg gaggatccag ccgtcgttga acgcatcgcc gacccagtcg
     1441 gtcaccaacc agagggtcac caccgcgagt ctcgggctca ccccgagcaa gatcaggaac
     1501 aaacaaccca taaccggcct ttctcgtcca tgccatccaa caccaggccc gccccgccgt
     1561 catcacctcg atcgggtgac gcccaccggg atcgccagtc gatcatccct ctcaggtgat
     1621 gcactgaccc actctttgtt gttcagttct caccatcacc gaggagagaa ccgtgcggat
     1681 cgtcaacagc ccgggtcggc tgccccaggc cgctgcgtat gctcgtcgca tcatgccaga
     1741 cccgagaagc acatgacggc gcaagaccca ttgggccggc ttatgcgtcg gcgggcgagc
     1801 cacacaggtt tggtcagggc ggccgccg
//
LOCUS       DATITW010000693         1779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_34455, whole genome shotgun sequence.
ACCESSION   DATITW010000693 DATITW010000000
VERSION     DATITW010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34455"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1767
                     /locus_tag="VLS86_09675"
     CDS             <1..1767
                     /locus_tag="VLS86_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HSO50805.1"
                     /translation="GEFLGRHGSVDDPAALRVTGKLSGSIGGGLDPCAALQRSLVLAP
                     GEEVEVVFLLGQGKNREEARALVERYRGTDPESVRAEVATRWNDILGTVRVKTPDRSF
                     DLMINQWLLYQTLACRVLARSAFYQSGGAFGFRDQLQDVMALVTPMRDVAREHLLRAA
                     AHQFEEGDVLHWWHGPSGKGVRTRISDDYLWLPYAVAHYLEVTGDRDILEETVPYLRG
                     MELTQDQDEIFFTPERSPIQGTLFDHCRRALDRARSRLGSRGLPLIGTGDWNDGFNRV
                     GREGRGESVWLGWFLEANLRQFADIAEEHGETELADGWREDALDLQASLEAEAWDGDW
                     YRRAFFDDGTPLGSSVNPECRIDSIAQSWSVIHGGAPDARARRAMDSVEEYLVHRGDG
                     LVLLFTPPFNDWDVDPGYIKGYLPGVRENGGQYTHASIWCVIAFATLGDGDRAGELFG
                     LLNPINHASTRAGIHRYRVEPYVAAADVYSEAPHVGRGGWTWYTGSAGWMYRAGIEWI
                     LGFRVRGTQLLIDPCIPRAWRGFEIVFRYHSSRYEIQVANPHGVTKGVAGLTLDGATL
                     APDSALELVDDGSTHQVQVTLG"
BASE COUNT          292 a          560 c          615 g          312 t
ORIGIN      
        1 ggggagttcc tgggcaggca cggcagtgtc gacgacccgg cagcgctgcg agtcaccggc
       61 aaattgtcgg gctcgatcgg cggcgggctc gatccctgcg ccgcactaca gcgctccctg
      121 gtcctggccc ctggcgaaga agtcgaggtg gtcttcctcc tcggccaggg caagaaccga
      181 gaggaggcgc gggcactggt cgagcgctac cgaggcacgg atcccgagtc ggtgcgggcc
      241 gaggtcgcca ccagatggaa tgacatactg gggacggttc gggtcaagac cccggaccgt
      301 tccttcgacc tgatgatcaa ccaatggttg ctctatcaga cgctcgcctg ccgggtgctg
      361 gcccggagcg cgttctacca gtcagggggc gccttcggct tccgggacca actgcaggat
      421 gtgatggcgc tggtgacccc gatgcgggat gtggcccgag agcatctgct tcgagctgcc
      481 gctcatcagt tcgaagaggg cgacgtcctc cactggtggc acgggccgtc gggcaagggc
      541 gtgcgaaccc gcatctccga cgactacctg tggcttccct atgccgtggc ccactatctg
      601 gaggtcaccg gagaccggga catcctggag gaaaccgttc cctacctgcg tgggatggaa
      661 ctgacccagg accaggacga gatcttcttc acccccgagc gatccccgat tcagggaacc
      721 ctgttcgacc attgccggcg agccctggac cgggcgcggt ccagactggg gagtcgtggt
      781 ctccccctga tcggcaccgg tgactggaac gacgggttca accgggtggg tcgggaggga
      841 cgaggcgaga gtgtgtggct gggctggttc ctcgaggcca acctgaggca attcgccgac
      901 atagccgagg agcacggtga gaccgagctc gccgacggct ggcgggagga tgctctggac
      961 ctccaggcca gcctcgaagc ggaggcatgg gacggtgact ggtatcggcg cgcgttcttc
     1021 gatgacggga cgccactcgg ctcgtcggtc aaccctgaat gtcgaatcga ctccatcgcc
     1081 caatcgtggt cggtgatcca cggtggggcc cccgacgccc gggcacggcg ggccatggac
     1141 tcggtggagg agtatctggt gcatcgaggt gacggtctgg tgctcctctt caccccgccg
     1201 ttcaacgatt gggatgtcga ccccgggtac atcaagggct atctccccgg cgtccgcgag
     1261 aacggtgggc agtacaccca tgcctccatc tggtgcgtca tcgccttcgc caccctgggc
     1321 gacggcgatc gggccggcga gctgttcggt ttgctcaacc cgatcaacca tgccagcacc
     1381 agagcaggga tccaccgtta ccgggtggag ccctacgtgg cggcagcgga cgtgtattcg
     1441 gaggctcccc acgtgggtcg tggcggctgg acctggtaca cgggctctgc gggttggatg
     1501 taccgggcgg gtatcgagtg gatactcggc tttcgggttc gggggaccca actcctcatc
     1561 gatccctgca tcccgcgggc gtggcggggc ttcgagatcg tgttccgcta ccactcctcc
     1621 cgatatgaga tccaggtggc caatccccat ggggtgacaa agggtgttgc cgggctgacc
     1681 ctggacggag cgacgcttgc tcccgactcg gctctggaac tggtcgacga cggctccacc
     1741 caccaggtgc aggtgaccct gggctgaccc agcgacggc
//
LOCUS       DATITW010000694         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_674698, whole genome shotgun sequence.
ACCESSION   DATITW010000694 DATITW010000000
VERSION     DATITW010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674698"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1353
                     /gene="lysA"
                     /locus_tag="VLS86_09680"
     CDS             76..1353
                     /gene="lysA"
                     /locus_tag="VLS86_09680"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HSO50806.1"
                     /translation="MSALPLDLLPETATVGPTGHLAIGGCPVSQLASEFGTPLFVYDE
                     DHVRRRCREAVTAFPDGVAYATKAFLCGAMAQLAHEEGMQLDVATGGEIHVALASGVP
                     ADRLVFHGNNKSETELRQALDAGVGRIVIDSTTEFEKIESLAAAGFPAPTVLVRVNPG
                     VEAHTHEFLQTGAADSKFGITIVGGEAATLVERARASDAMRFIGIHAHVGSQVFDVSS
                     FERAIGVIAGFAAPLGIEELSIGGGLGVAYIEGEKAPTITEWGAAIANAVSASGLEAR
                     IVAEPGRSIVARAAVTIYTVGTIKQIPGVRTYVSVDGGMSDNPRPVLYGSGYEVFVPT
                     ATSADRTREIRLVGKHCESGDVLAWSARVPDELAPGDLLATPVTGAYGHSMGSNYNMV
                     RRPAVVFVRDGEARLVVRRETHQDLLARDLVAG"
     gene            complement(1356..>1538)
                     /locus_tag="VLS86_09685"
     CDS             complement(1356..>1538)
                     /locus_tag="VLS86_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50807.1"
                     /translation="GFVARDSDQFNFITATGGECPGQTGVYEATITASDLVLVVVDDP
                     CQLRSAGFAQAFARGD"
BASE COUNT          259 a          496 c          522 g          261 t
ORIGIN      
        1 aaaggcgccg cttgggcctg accacagcag gcgcgcccgg ggcgccaatc ctccactgtt
       61 aggttgcctt ccccgatgtc cgctctcccc ctcgacctcc tgcccgaaac tgcgaccgtg
      121 ggccccaccg ggcacctggc catcggcggt tgcccggtgt cacagctcgc atccgagttc
      181 ggcacacctc tgttcgtcta tgacgaggac catgttcggc gacggtgccg cgaggcagtc
      241 accgccttcc ccgacggtgt ggcctacgcc accaaggctt tcctgtgcgg agccatggcc
      301 caactggcgc atgaggaggg aatgcagctg gatgtggcga caggtggcga gatccacgtc
      361 gccctcgctt cgggtgtccc cgccgatcgc cttgtcttcc acggcaacaa caagtcggag
      421 accgagctgc gacaagccct ggacgccggg gtcggccgca tcgtgatcga ttcgaccacg
      481 gagttcgaga agatcgagtc gttggccgca gccggcttcc ctgctcccac cgtgctggtg
      541 agggtcaacc ccggtgtcga agcccacacc cacgagttcc tgcaaaccgg ggccgcggac
      601 tccaagtttg ggatcaccat cgtcgggggc gaggcggcga cactcgtcga gcgggcccgc
      661 gcctcggacg cgatgagatt catcgggatc catgcccacg tcgggagcca ggtgttcgat
      721 gtgagctcct tcgagagagc gatcggggtg atcgccgggt tcgctgcccc actggggatc
      781 gaggaattgt cgataggagg gggattgggg gtggcataca tcgaaggtga gaaggcgccc
      841 acgatcaccg aatggggtgc ggccatcgcc aacgcggttt ctgcgtcggg cctcgaggct
      901 cggatcgtcg ccgagccggg caggtcgatc gtggctcggg ccgcggtcac catctacacc
      961 gtcgggacca tcaagcagat cccaggtgtg aggacctatg tctcggtcga cggggggatg
     1021 agcgacaacc cgagaccggt tctgtacggc agcggctacg aagtgttcgt tccgaccgcg
     1081 accagcgctg accgaacccg cgaaatacgt cttgtgggca agcactgcga atcgggcgac
     1141 gtcctcgcct ggagtgcccg ggtgccggac gaactggccc cgggcgacct ccttgccaca
     1201 ccggtgaccg gtgcctacgg gcattcgatg ggctcgaact acaacatggt ccgccggccg
     1261 gcggtggtct tcgtccgcga tggcgaagcc cgcctggtgg tcagacgcga gacgcatcag
     1321 gacctgctgg cccgtgattt ggtggccgga taggttcaat cgcccctggc gaaagcctgg
     1381 gcgaagcctg cggaccgcaa ctggcatggg tcgtcgacga ctaccagcac gaggtcggag
     1441 gcggtgatcg tggcctcgta gacaccggtc tgaccggggc actctccgcc ggtcgcggtg
     1501 atgaagttga actggtcgct gtcccgggcg acgaaacc
//
LOCUS       DATITW010000695         1798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_635474, whole genome shotgun sequence.
ACCESSION   DATITW010000695 DATITW010000000
VERSION     DATITW010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635474"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1263)
                     /locus_tag="VLS86_09690"
     CDS             complement(22..1263)
                     /locus_tag="VLS86_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012942899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSO50808.1"
                     /translation="MSELIETNELVLLVLGLAAFGVVLLPHVLSRRPLSYPIIFVAAG
                     ALLFSLPLGLPEPDPIEYAEATETLTEIGVITALMGAGLALNRVPGWKSWASTTRLLA
                     ITMPLTIAAVAGLGWWALGLTPAAAILLGAVLAPTDPVLASDVQVPAPDRDDTDDDEV
                     RFALTSEAGLNDGLAFPFTNLAILIAVYGLAPSGWLGEFLLVDVLYRVTIGVLGGLLV
                     GRVLAWAIFTRRSPLPGIRDGMIAFAATLIAYAGSEIVGGYGFLAVFVAAVVIRNQER
                     KHSYLEVMHQFSDQIERLLSAIVLIHFGGALTSGILGSLDWQGVLAAVVIVLIVRPIA
                     GIAGLARLPMPDRERAAIAFFGIRGVGSFYYLAYASNQVEFIQIEQLWAVTALVVVIS
                     VVVHGVSATPAMAYIDRRAES"
     gene            1375..1788
                     /locus_tag="VLS86_09695"
     CDS             1375..1788
                     /locus_tag="VLS86_09695"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSO50809.1"
                     /translation="MHIVDQHFAAENAHDVAATLATYTDDIVWDDVTHPLSPVHGKEA
                     VGAVYTDIIEAIPDVNFVSIRRFTSEDGEWVVDVSDVTGHVEGDWAGVSGGGAPVEIR
                     ILHLFQLRDGLIAYENTWFDSAAVLRQVEAWKTSH"
BASE COUNT          373 a          593 c          567 g          265 t
ORIGIN      
        1 gctcaggctg ccgccgcccg atcagctctc agcccgacgg tcgatgtatg ccatggccgg
       61 cgtggcggag actccgtgga ccaccaccga gatcaccacc accagtgcgg tgaccgccca
      121 caactgctcg atctggatga attcgacctg gttggaggcg taggcgaggt agtagaacga
      181 gcccactccc cggatcccga agaaggcgat cgcggcacgt tctcgatcgg gcatcggcaa
      241 acgtgccagg ccggcgatcc cggcaattgg gcggacgatg agcacgatca ccaccgcggc
      301 cagtactccc tgccagtcca gggacccgag gatcccgctg gtcaacgccc ctccgaagtg
      361 aatgagcaca atcgcactca acagccgctc gatctggtcg gagaactgat gcatgacctc
      421 caggtaggag tgctttcgct cctgattgcg gatgacgacg gcagccacga acacggcgag
      481 gaacccgtac ccacccacga tctcggaccc ggcataagcg atcagggtgg cggcgaaggc
      541 gatcatcccg tccctgatcc cgggcagagg cgagcgccgg gtgaagatgg cccaggccaa
      601 caccctgccc accagcaatc cacccaagac cccgatggtg accctgtaga gcacgtcgac
      661 caggaggaat tcgccgagcc aaccggatgg cgccaggcca tacaccgcga tcaggatggc
      721 gagattggtg aagggaaacg ccagcccgtc gttgaggcca gcttccgaag tgagcgcgaa
      781 acgcacctcg tcatcatcgg tgtcatcccg gtccggggcc ggaacctgga cgtcactggc
      841 cagcaccggg tcggtcgggg cgagaaccgc gccgagcagg atcgccgccg ccggtgtcaa
      901 cccgagtgcc caccagccga gcccggcgac cgcggcgatc gtcaggggca tggtgatcgc
      961 cagcaatctg gtggtcgatg cccaggactt ccagcccgga acccgattga gagcgagccc
     1021 tgcacccatc agggcggtga tcaccccgat ctcggtgagg gtctcggtgg cttccgcgta
     1081 ctcgatcggg tctggctccg ggagccccaa cggcagtgag aagagaagag ctcctgctgc
     1141 cacgaagatg atcggatagg aaagtggccg ccgactcaac acatggggta gcaacaccac
     1201 cccaaaggcg gcgagaccga gcaccagaag caccaactcg ttcgtctcga tgagctcact
     1261 cacctccgac gcccatccgg agcgcgccgt gcaacggatc gagagtgacg catcgactca
     1321 ttcaaacagg caacggatcg ggcctggagg gccgcggtcg gctagaaagc caccatgcac
     1381 atcgtcgatc agcactttgc cgccgagaac gcccacgacg tcgccgccac gctggcgact
     1441 tacaccgacg acatcgtctg ggacgacgtg acccacccac tgagcccggt gcacggcaag
     1501 gaggcggtcg gcgcggtcta tacggacatc atcgaagcca tccccgacgt caacttcgtc
     1561 tcgattcggc gtttcacctc ggaggacggg gagtgggtgg tcgacgtctc cgatgtcacc
     1621 ggtcatgtcg agggcgactg ggccggggtc tctggcggcg gggcaccggt cgagatcagg
     1681 atccttcacc tgttccaatt gcgcgacggc ctcattgcat acgagaacac ctggttcgac
     1741 tcggcggcag tcctgcgcca ggtcgaggct tggaagacat cgcactgagg tctagagc
//
LOCUS       DATITW010000696         2909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_234746, whole genome shotgun sequence.
ACCESSION   DATITW010000696 DATITW010000000
VERSION     DATITW010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234746"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VLS86_09700"
     CDS             <1..861
                     /locus_tag="VLS86_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HSO50810.1"
                     /translation="ELPTRSPEVAIDNPVFRPPFVGTRVVKGIPLDVIAEYLNETALF
                     RNQWQYRPETGERDPEFKERMAPLLREQIAKARADDLLHPKVVYGYFPANADGDDLVI
                     WDDERAQTERTRFSFPRQQEDPYLCIADFFRPVGEEVDHAAFHIVTMGGRISEVTKEL
                     FEADRYTDYLLLHGLGVELAEALAEHWHHRIREEWGFASEDGPSLLGLFRQQYRGGRY
                     SWGYPACPDLEDNEKVAYLLGADRIGITVGEETSYQYQPEQTTSAVICHHPQAKYFVA
                     RKPREKKDAD"
     gene            851..1642
                     /locus_tag="VLS86_09705"
     CDS             851..1642
                     /locus_tag="VLS86_09705"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HSO50811.1"
                     /translation="MLTEANHWLRIAEINPPELPNLQHRSLSPTWSHVLVTDNVFGRI
                     RVSPYAFSARITHDTPNVHPTVVVSTRDRNILAIESEVRGALGNGVDSFLVVIGDTVP
                     HVDHLAHHYEIVEHLRELQENLPNFEVGMPTRFREWQFRRRIELGAQFFVTGPILDPE
                     VVEEHVARLNLKPEDPPVFLMIIPPFSPAWVEQMETIGAVPVTDGLKQRLSELAPDVA
                     RKLAWDLTTEMERRARDAGAAGVVLMGLKFDSLVSEAAENWPHPG"
     gene            1785..>2909
                     /locus_tag="VLS86_09710"
     CDS             1785..>2909
                     /locus_tag="VLS86_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSO50812.1"
                     /translation="MTLLLVVLVVGLAFANSVGASRVASNAKDLHWTNAALGTAALTR
                     AALVQATTFSELESTGLATSDDLTAALDEALDARDRLTELRDGGGANTAVADLTHFLT
                     KVGVATSALEDGDFGTAKTLLMSEMETAYVDLADSLMAEQANIQLQIDANTAGAARMT
                     GFIQFFLMLAIPASAVLIYRTIAKRQIREYRLKVEVELEAERAVGRAKDEFIAGLSHE
                     LRTPLTSIYGFAEILTDGGAEDPATSRELAQIIANESAEMTRMVDDLLAAARLESTGI
                     GIELAPTRVSDVVSSALLPFQRAGLVVKRGGEEAVVATDAARLRHVLINLISNAARHG
                     GPKVGIEVSAGDGVVDIEVWDDGSGVPDDQLEKLFETFVHN"
BASE COUNT          534 a          926 c          943 g          506 t
ORIGIN      
        1 gaactcccaa cgcgatcgcc tgaggtggcc atcgacaatc cagtgtttcg gccacccttt
       61 gtcggaacaa gggtggtgaa ggggatccct ctcgatgtga tcgctgagta tctcaacgag
      121 accgccctct ttcgcaacca gtggcagtat cgacccgaga cgggggagcg ggacccggag
      181 ttcaaggagc ggatggcgcc gctgctccgg gagcagatcg ccaaggcgcg tgccgacgac
      241 ctgctgcacc caaaggtcgt ctacgggtac ttcccggcga atgccgacgg cgacgatctg
      301 gtcatctggg acgacgagcg ggcccagacc gagcggacca gattcagctt tccccgccag
      361 caggaggacc cctatctgtg catcgcagac ttcttcagac cggtcgggga ggaggtcgac
      421 catgctgcct tccacatcgt caccatgggg ggccggatct cggaggtcac caaggagctg
      481 ttcgaagcgg atcgctacac cgattacctg ttgctccacg gtctcggggt cgagctggcc
      541 gaggcactcg ccgagcactg gcaccaccgg atcagggagg aatgggggtt cgcctccgag
      601 gacgggccct cattgctcgg gttgttccgt cagcagtacc ggggtgggcg ttactcgtgg
      661 ggctatccgg cgtgccccga cctcgaggac aacgagaagg tggcctattt gttgggggca
      721 gatcggatcg ggatcaccgt cggcgaggag acctcctacc agtaccaacc ggagcagacc
      781 acatcggcgg tgatctgcca ccacccccag gcgaagtact ttgtcgcccg caagccaagg
      841 gagaagaaag atgctgactg aggccaacca ctggcttcgc atcgcggaga tcaacccgcc
      901 cgagctgccc aatctccagc acaggagcct gtcaccgacc tggagccatg tcttggttac
      961 ggacaacgtc ttcgggcgga tccgtgtctc gccatatgcg ttctcggccc ggatcaccca
     1021 tgacactccc aacgtccatc ccacggtggt ggtctccacc cgagaccgaa acatcctggc
     1081 catcgagtcc gaggtgcgtg gcgcgctggg aaacggcgtc gactcgttcc tggtcgtgat
     1141 cggcgacacc gttccccacg tcgatcatct cgctcatcat tacgagatcg tggagcacct
     1201 gagagagctc caggagaacc tgccgaactt cgaggtgggc atgcccaccc gtttccggga
     1261 gtggcagttc aggcgacgga tcgagctcgg ggcccagttc ttcgtcaccg gccccatcct
     1321 ggacccggag gtggtggagg aacacgtggc ccggctcaat ctcaagcccg aggacccacc
     1381 ggtgtttctg atgatcatcc ctccgttcag cccggcctgg gtggagcaga tggagacgat
     1441 cggcgccgtg ccggtgaccg acgggttgaa gcagcggttg tcggagctgg ccccagacgt
     1501 ggcccgcaag ctggcatggg atctgaccac cgagatggag cgccgggctc gcgacgccgg
     1561 ggcggccggc gtggtcctga tgggcttgaa attcgattcg ctggtcagcg aggcggcgga
     1621 gaactggccc catccgggct gaaccggtct ctcgtctccg cggtagtcca tggatcgcgc
     1681 ggcctataaa gaaatcgcgc ggagaatgcc gataaccgac gatggggtcg gagcgtccgc
     1741 ttcgtgcaac tgcgccaagc cgcgcagtcg gcgtcgccat cggggtgacg ctgcttctgg
     1801 tcgttctcgt agtcggtctg gctttcgcca actcggtcgg tgcatcgaga gtcgcctcca
     1861 acgccaagga ccttcactgg accaacgccg ccctgggcac cgccgcactg acacgggcgg
     1921 ccctggtcca ggccacgacc ttctccgagc tggagagcac ggggttggcg acgagcgacg
     1981 acctcacggc ggccctcgac gaggcattgg atgcccggga ccgtctcacc gagctccgtg
     2041 acggcggagg cgccaacacc gctgtcgccg acctgaccca tttcctgacc aaggtcggag
     2101 tggccacttc ggccctcgag gatggggact ttggcaccgc gaagacgctg ctgatgtctg
     2161 agatggagac cgcctacgtc gaccttgccg actccctcat ggccgagcaa gccaatatcc
     2221 agttgcagat cgacgccaac accgcgggag cggcgaggat gaccgggttc atccagttct
     2281 tcctcatgct ggccatccca gcttccgccg ttctcatcta tcggaccatc gccaaacgac
     2341 agatccgtga gtatcgactc aaggtcgagg tcgagctcga ggcggaacgg gccgtgggca
     2401 gggccaagga cgagttcatc gccggtctgt ctcacgaatt gcgcacccca ttgacctcga
     2461 tctacgggtt tgccgagatc ctcaccgatg gcggagcgga ggatccggcc acatcacgcg
     2521 agctggcaca gatcatcgcc aacgaatcgg cggagatgac gaggatggtg gacgatctcc
     2581 tggcggcggc tcgcctggag agcaccggaa tcgggataga gctggctccg acccgggtct
     2641 cggacgtcgt ctcctctgcc ctcctcccgt tccagcgcgc cgggctcgtg gtcaagcgcg
     2701 gcggggagga ggccgtggtc gccaccgatg cagctcgctt gcgtcatgtg ttgatcaacc
     2761 tgatctccaa tgccgcccgt catggagggc cgaaggtcgg aatcgaggtg tcagcgggcg
     2821 atggcgtggt cgacatcgag gtctgggacg acggctccgg ggtccccgac gaccagctag
     2881 agaagctgtt cgagaccttc gtacacaac
//
LOCUS       DATITW010000697         1875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_194151, whole genome shotgun sequence.
ACCESSION   DATITW010000697 DATITW010000000
VERSION     DATITW010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194151"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..188
                     /locus_tag="VLS86_09715"
     tRNA            115..188
                     /locus_tag="VLS86_09715"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:149..151,aa:Pro,seq:tgg)
     gene            214..284
                     /locus_tag="VLS86_09720"
     tRNA            214..284
                     /locus_tag="VLS86_09720"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:246..248,aa:Gly,seq:tcc)
     gene            325..398
                     /locus_tag="VLS86_09725"
     tRNA            325..398
                     /locus_tag="VLS86_09725"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:359..361,aa:Arg,seq:tct)
     gene            422..496
                     /locus_tag="VLS86_09730"
     tRNA            422..496
                     /locus_tag="VLS86_09730"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:457..459,aa:His,seq:gtg)
     gene            559..>1875
                     /gene="tig"
                     /locus_tag="VLS86_09735"
     CDS             559..>1875
                     /gene="tig"
                     /locus_tag="VLS86_09735"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HSO50813.1"
                     /translation="MPTTVTETGPFERLVTFQLTEDQIAAGKVATARKLSQDLKLKGF
                     RPGRAPLPVVEAAVGPDRLRSEVIDDLVPPVLTDILKDEEIRPAVTPQLESLNDVDGG
                     VEVEVRVTLWPTIELPNYRNRNVEVTSPEVTDEDLDTQIERILEQFATVEEVTRPAAV
                     GDFVSIDLKATKENEKVEEASASDLLYEVGSGVFLDGIDERLEGTEADTTFAFDAPLP
                     EGFGERAGEVVTFEVTVNEVKERVLPELDDGWVDENTEFETVQALKDELRERLTEAKI
                     QAVSRQFSERALNTLVEQVEVELPEALVRSEMDDHLHRFVHRLEDNELTLDDYFQASG
                     VDQDQFLEDLRQQAQLSLRNQLVLEAVARDAEIEVTPEDVSTVIQGLAARSGDPVAYL
                     KAFSQSGQELALAGDILRNRALEAILSEASAVDEEGNPVDLKPMVTE"
BASE COUNT          379 a          565 c          609 g          322 t
ORIGIN      
        1 gacaatcggt cccagtccgc gatccgagcc accaattccg gctccacccc catgttcccg
       61 aagataggga tggggtggga cagaaacgcg aggggaatta cgctgagagc tcaacgggct
      121 gtggcgcagc ttggtagcgc gcgacgtttg ggacgtcgag gtcgtcggtt cgagtccgac
      181 cagcccgacg aaggccgtaa actgaccctc cctgcgggtg tagctcaatg gtagagctcc
      241 agccttccaa gctggtggtg agggttcgat tcccttcacc cgctcgattg ggttgccctt
      301 cagggcggta tgtccaaggc ccttgcccct gtagctcaat gggatagagc agcggacttc
      361 taatccgcag gttgggggtt cgagtcctcc cgggggcgca aaggacgacc cattcgacat
      421 ggtggccgta gctcagtccg gttagagcac ctggttgtgg tccaggaagt cgcgggttcg
      481 aatcccgtcg gtcacccaaa cagcgccgcc cggtgtcagc cgggcggcga acgatcaccg
      541 tctagagcca ggaagagaat gccaacaacc gtcaccgaga ccggtccctt cgagcgtctg
      601 gtcacgttcc aactcaccga ggaccagatc gcagccggca aggtggcgac ggcgcggaag
      661 ctctcccagg acctgaagct caaggggttt cgccccggcc gcgccccgct ccccgtcgtc
      721 gaagccgcag tcggtccgga tcgtctccgc agtgaagtga tcgacgacct ggtgccgccg
      781 gtgctgaccg acatcctcaa agacgaggag atccggcctg ccgtgacgcc acagctggag
      841 agcctcaacg acgtcgacgg tggggtggag gtcgaggtcc gggtcacgct gtggcccacc
      901 atcgagctcc ccaattacag aaaccgaaat gtcgaagtca cctccccgga ggtgaccgac
      961 gaagatctgg acacccagat cgagcgaatc ctcgagcagt tcgccaccgt ggaagaggtc
     1021 acccggcccg cggccgtcgg cgacttcgtt tctatcgacc tcaaggcgac caaggagaac
     1081 gagaaggtcg aagaggcctc ggcgtcggac cttctctatg aagttggctc tggagtcttt
     1141 ctcgatggca tcgacgagcg actcgagggg acggaggcgg acacgacctt cgcattcgac
     1201 gccccgctgc cggagggctt tggagagcgt gcaggggagg tcgtgacctt cgaagtcacg
     1261 gtcaacgagg tcaaggagcg agtcctgccc gagctcgacg acgggtgggt ggacgagaac
     1321 accgagttcg aaaccgtcca ggcgttgaaa gacgagctcc gcgagcgtct caccgaggcc
     1381 aagatccagg cagtgtcccg ccagttcagc gaacgggcgc tcaacaccct ggtcgaacag
     1441 gtcgaggtcg agcttcccga ggcgctagtc cggtcggaga tggacgatca tctccatcgt
     1501 ttcgttcacc gtctggagga caacgagctc accctggatg actacttcca ggcatcgggt
     1561 gtggatcagg accagttcct cgaggacctg agacagcagg cgcagctctc gcttcggaat
     1621 cagctggtcc tggaagcggt ggcccgagat gccgagatcg aggtgacgcc ggaggatgtc
     1681 tcaaccgtga tccaaggcct ggccgcgagg tccggtgacc cggtcgccta tctgaaggcc
     1741 ttctcccaga gcgggcaaga attggcgctg gccggtgata ttctcagaaa cagggcgctg
     1801 gaagccattc tctccgaggc cagtgcggtc gatgaagagg gcaatccggt cgatttgaaa
     1861 cctatggtga ccgaa
//
LOCUS       DATITW010000698         1894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_514679, whole genome shotgun sequence.
ACCESSION   DATITW010000698 DATITW010000000
VERSION     DATITW010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514679"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VLS86_09740"
     CDS             complement(<1..334)
                     /locus_tag="VLS86_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSO50814.1"
                     /translation="MKLIDRLLPLQPGQRVLEVGCGQGHLTGRLAERGVDVVGIDANP
                     NAPVVSGSDRVLCMNAENLDFADSEFDAIVSVHAIEHIPPLEEAVAEMARVLKPGGRA
                     LFIYPAEPI"
     gene            complement(402..1634)
                     /locus_tag="VLS86_09745"
     CDS             complement(402..1634)
                     /locus_tag="VLS86_09745"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSO50815.1"
                     /translation="MTSITLIRHAESQANVDHIWNGQIDGPLSAGGEATLDVLGERLG
                     QQRFDVVISSPLERARRTAQAFAPDFEVSEEYMELDIGRWEGMTRDQVMAEAGDDLRE
                     AVLGRELAMGGTGESLNALHRRAVLALEALAKRLGEDGRAAVVTHGGFIQEVLRGHLA
                     GRSRRVHTFAGNTSLTRLVWSWGRPRLAVFNDMGHLAPRPAEVESHLEKGEPVIALIR
                     HGQTRANTEGRWQGQGDWGLDDHGHQQAAALRDWYGLASTVYASPLGRAQSTAGYVAS
                     NGIVTVDGLKEIAMGQWEGLTSDEIFEKWPGQMKTIYEEGIDLKRGETGESWGELTGR
                     FRATVHGVAPSLEGPTVVVAHGGAIRAYVSSLTVTSDTHSESLYTPGNTSVTHVAMTA
                     EGPLLLDYGVAAHVEKLS"
     gene            complement(1715..>1894)
                     /locus_tag="VLS86_09750"
     CDS             complement(1715..>1894)
                     /locus_tag="VLS86_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HSO50816.1"
                     /translation="AKKFIESGAFGGKGSDAHKAAVIGDTVGDPFKDTSGPAMNIVIK
                     VMTIVSLIFASAFVG"
BASE COUNT          308 a          648 c          618 g          320 t
ORIGIN      
        1 ggatcggctc cgccggatag atgaacaggg ccctgccccc cggcttcagt acccgcgcca
       61 tctcggccac cgcctcctcc agcggcggga tgtgctcgat ggcgtggacc gagacgatgg
      121 cgtcgaactc ggagtcggcg aagtccaggt tctcggcgtt catgcagagg acacgatccg
      181 aaccggagac cacaggtgca ttggggttgg cgtcgatacc gacgacatcg acgccccgtt
      241 cggcgagacg accggtgaga tgtccctgac cacaaccgac ctcgagcacc cgctgcccgg
      301 gctggagagg aagcaggcga tcgatcagct tcaagtgaga tgggtcccac cgggtgacgg
      361 tggcgtagcg gtgagagtcg tacccggacc tcgaggggag gctaggagag cttctcgaca
      421 tgagcggcga ccccgtagtc gagcagcaag gggccttcgg cggtcatcgc cacgtgggtc
      481 accgacgtgt tgcccggcgt gtacagcgac tcggagtggg tgtcggaggt gacggtgagc
      541 gaggagacgt aggcgcgtat cgccccaccg tgggcgacca caaccgtcgg cccctccagc
      601 gagggcgcca ccccgtggac cgtcgcccga aagcgcccgg tgagctcacc ccagctctcc
      661 ccggtctcac ctcgtttgag gtcgatgcct tcctcgtaga tcgtcttcat ctgccccggc
      721 cacttctcga agatctcgtc ggaggtgagc ccctcccatt gccccatggc gatctccttg
      781 agcccatcca cggtcacgat cccgttgctt gcgacgtagc cggccgtcga ctgggcacgg
      841 ccgagggggg acgcatagac ggtgctcgcc aggccatacc aatctcgaag cgcggcggcc
      901 tgctggtgcc cgtgatcgtc cagtccccag tcgccctgcc cctgccagcg gccctcggtg
      961 ttggcccggg tctggccatg acgaatcagg gcgatcaccg gctctccctt ttccaagtgc
     1021 gactcgacct cggccggtcg tggggcgaga tggcccatgt cgttgaacac ggccagtcgg
     1081 gggcgtcccc aggaccagac cagacgggtg agcgaggtgt tcccggcgaa ggtgtgcacc
     1141 cgtcggctcc tcccggcgag gtgaccacga aggacctcct ggatgaaccc cccatgcgtc
     1201 accaccgccg ccctgccatc ctcgccgagc cgcttcgcca gggcctcgag ggccagaact
     1261 gcgcggcggt gcagggcatt gagactctcc ccggtgccgc ccatcgccag ctcgcgaccg
     1321 agcacggcct ctctgagatc gtccccggcc tcggccatga cctggtcccg ggtcatcccc
     1381 tcccatctcc cgatgtcgag ctccatgtac tcctcggaga cctcgaagtc cggggcgaag
     1441 gcctgtgccg tgcgtcgggc gcgctcgagg ggcgagctga tgacaacgtc gaaccgttgc
     1501 tgcccaagac gctcgccgag gacatcgagg gtggcttctc ccccggcgct gagtgggccg
     1561 tcgatctgcc cgttccagat gtggtcgacg ttggcctggg actcggcgtg acggatcagg
     1621 gtgatcgagg tcatttgcgt gttaggtggt aggtcgtggg atttgccaag tcaagaccgg
     1681 atcccgcacc gccaccccgg gacctgccgc ggtctcaacc cacgaaggcg gaggcgaaga
     1741 tcagcgagac gatggtcatc accttgatga cgatgttcat cgccggcccc gaggtgtcct
     1801 tgaacgggtc acccaccgtg tcaccgatca ccgccgcctt gtgcgcatcg gagcccttgc
     1861 cgccaaaagc gccggactcg atgaacttct tcgc
//
LOCUS       DATITW010000699         1740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_74855, whole genome shotgun sequence.
ACCESSION   DATITW010000699 DATITW010000000
VERSION     DATITW010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74855"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VLS86_09755"
     CDS             <1..696
                     /locus_tag="VLS86_09755"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HSO50817.1"
                     /translation="RGQEIAEAAKRAGVSVMYEAAVGGGIPVIRPLAESLAGEDIDTV
                     SGIVNGTTNFILTAMAEEGRSYQDALNEAQRLGYAETDPADDVSGRDAAFKTAILASL
                     AFGERVPPWEVECEGIEGVSAQDVADAADRGEVIKLIGSARRVNGVVKATVSPKSLPK
                     DHPLAGISGVTNAIFIEGPSVGRLLFTGPGAGGSATASAMLGDLIQIASRGRGPRQRS
                     PHDPKPKRQVLHR"
     gene            complement(693..>1740)
                     /locus_tag="VLS86_09760"
     CDS             complement(693..>1740)
                     /locus_tag="VLS86_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HSO50818.1"
                     /translation="ARAGITPVATLSHWDLPQALKDRGGWLHPESVAWVTEYARVAKA
                     ALGDMVSWWATLNEPWCSAFLGYSAGEHAPGLTDTAGSLVVAHHLMLAHHSAIRVMRE
                     TSARDDDRLGIVLNLIPAWPQSDTEEDRLAARSVDAVSNRLFTEAVFHGRYPDEVLEI
                     FDRYQVSDRIDLEALLGVVRPIDFLGVNYYNINHVEHAPGAESMGAWPGSWEARIASP
                     PGMLTEMNWGVEPEGLTWMLERVAREYPGVPTLVCENGGAYGDEPGPDGFVNDVARIQ
                     YLQGHIEAVRVAIERGADVRGYFVWSLLDNFEWARGYAKRFGIVRVDYETMARTIKAS
                     GLWYRDFLAGRSIV"
BASE COUNT          299 a          589 c          577 g          275 t
ORIGIN      
        1 cgaggccagg agatcgccga agcggccaag cgggccgggg tctcggtcat gtacgaggca
       61 gccgtcggtg gtgggatccc ggtgatccgg ccgctggccg agagtctcgc cggtgaggac
      121 atcgacaccg tcagcggaat cgtcaacggc accaccaatt tcatactcac cgccatggcc
      181 gaggagggcc gcagctacca ggatgccctc aacgaggcgc agcggctggg atacgccgaa
      241 accgacccgg ccgacgatgt ctccgggagg gatgccgcgt tcaagaccgc gatcctggcc
      301 agtctcgcct tcggcgagag ggtcccgccc tgggaggtgg agtgtgaggg gatcgaaggg
      361 gtgtcggcgc aggacgtggc cgatgccgcc gatcggggcg aggtgatcaa gctcatcggg
      421 tcggcgcggc gtgtgaacgg ggtggtcaag gccaccgttt cgccaaagag cctccccaaa
      481 gaccaccctc ttgccgggat ctccggagtg accaacgcca tcttcatcga agggccgtcg
      541 gtgggtcgtc ttctcttcac cgggcccggg gccgggggct cggcgacggc gagcgccatg
      601 ctcggcgacc tgatccagat cgcttcacgg ggccgggggc cgcgccagcg gtcaccacac
      661 gatcccaagc cgaagcggca ggtcctgcac cgctagacga ttgatctccc ggcgaggaag
      721 tcgcggtacc acagcccgct ggctttgatc gtcctggcca tggtctcata gtcgacccgc
      781 acgatcccga agcgcttggc gtaaccgcgc gcccattcga agttgtcgag aagggaccag
      841 acgaagtagc cccggacgtc cgctccccgt tcgatcgcca cccgcaccgc ctcgatgtgg
      901 ccctggaggt actggatgcg tgccacatcg ttgacgaacc cgtcgggccc gggctcgtct
      961 ccgtaggcgc ctccgttctc gcacaccaaa gtcgggacac ctgggtactc cctggcgaca
     1021 cgctccagca tccaggtcag cccttcgggc tccacacccc agttcatctc ggtcagcatc
     1081 cccggtggtg aggcgatccg ggcctcccag ctccccggcc aggcacccat cgactcggca
     1141 ccgggagcat gctcgacgtg gttgatgttg tagtagttga cccccaggaa gtcgatgggt
     1201 cgcaccaccc cgagcagggc ctccaggtcg atgcgatcac tcacctggta ccggtcgaag
     1261 atctcgagga cctcgtcggg atacctgccg tgaaacacgg cttcggtgaa caaccggttg
     1321 ctgacggcat ccacggaccg ggccgccaga cgatcctcct cggtgtccga ctgaggccag
     1381 gccgggatca ggttgagcac gatcccgaga cggtcgtcgt ctcgggccga ggtctcacgc
     1441 atcaccctga tcgccgagtg atgggccagc atgaggtggt gggctacgac cagcgagccg
     1501 gcggtgtcgg tgagacctgg cgcatgctcg ccagcgctgt agccgaggaa tgccgagcac
     1561 cagggctcat tcagcgtggc ccaccagctc accatgtccc ccaaggctgc cttggccact
     1621 cttgcgtact cggtgaccca ggccaccgac tcgggatgga gccagccccc ccgatcctta
     1681 agcgcctggg gcaggtccca gtgggagagg gtcgccaccg gggttatccc ggcacgggcc
//
LOCUS       DATITW010000700         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_154258, whole genome shotgun sequence.
ACCESSION   DATITW010000700 DATITW010000000
VERSION     DATITW010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154258"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VLS86_09765"
     CDS             complement(<1..684)
                     /locus_tag="VLS86_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HSO50819.1"
                     /translation="MARAVGIDLGTTNSVIAVLDGGDPTVIPNAEGGRTTPSVVSFKD
                     GEVLVGEVAKRQAITNPDQTVRSVKRHMGTDWTIEIDDKKLTAQEISARILMKLKRDA
                     ESYLGDKVTDAVITVPAYFNDSQRQATKEAGQIAGLNVLRIINEPTAASLAYGLDKVA
                     DHTILVFDLGGGTFDVSVLEIGDGVFEVKATSGDTQLGGDDWDQKVIDWLVEGFKNDH
                     GVDLGKDRMA"
     gene            complement(812..1237)
                     /locus_tag="VLS86_09770"
     CDS             complement(812..1237)
                     /locus_tag="VLS86_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSO50820.1"
                     /translation="MRSWAEGALALGLTLVLGLGLWLFTGESIGADPTATTTAIAFDS
                     EAAARGEAIAAATGCLACHTIDGASGTGPTWKGLAGASRPLVTGESVLADDAYLFSAV
                     VDPAAQVVAGFDPVMPVTYSENLTEDEINDLVEYIRSLS"
     gene            1416..1928
                     /locus_tag="VLS86_09775"
     CDS             1416..1928
                     /locus_tag="VLS86_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSO50821.1"
                     /translation="MTGIDVALLLLRLWAGIVIIAHGVNHGRTLEGTAKWFASKGFKS
                     AGLNARISALNEIAIGVALIAGLLTSVAAAGLVATMFVAFWSIHRFVGFFNFRRPDEG
                     YEYVATLAVIGTAVAIAGPGRYSVDEAIGIAGNLDGWVGAAIVGAGLLVAVGQLATFW
                     RKPAGDGGSA"
BASE COUNT          332 a          678 c          686 g          400 t
ORIGIN      
        1 ggccatgcgg tccttgccca ggtcgacgcc gtggtcgttc ttgaagccct cgaccagcca
       61 gtcgatgacc ttctggtccc aatcgtcgcc accgagctgg gtgtcgcccg aggtggcctt
      121 gacctcgaac acgccgtcgc cgatctccag caccgacacg tcgaatgtgc caccaccgag
      181 gtcgaagacg aggatcgtgt ggtccgccac cttgtccagc ccgtaggcca gcgaggccgc
      241 ggtcggctcg ttgatgatgc gcaggacgtt gagccccgcg atctggccgg cttccttggt
      301 cgcctggcgc tgggagtcgt tgaaataggc gggaacggtg atgacggcgt cggtgacctt
      361 gtcaccgagg tacgactcgg cgtcccgctt caacttcatc aggatccgcg ccgagatctc
      421 ctgggcggtg agcttcttgt cgtcgatctc gatcgtccag tcggtgccca tgtgacgttt
      481 caccgagcgg acggtctggt cggggttggt gatggcctgt ctcttggcta cctcgccaac
      541 cagcacctca ccgtccttga acgagacgac cgatggggtg gttcgccccc cctcggcgtt
      601 ggggatgacc gtggggtcgc ccccgtcgag caccgcgatg acactgtttg tggtgccaag
      661 atcgatgcct actgcgcgtg ccattgagag atcctccgat tcaggtttga gtctgttgcg
      721 atactacaac ctgagtcggg tagtgtcata ttcccgtgac aatcctctga tcggagtctg
      781 accgtggagc accatcgatc aaccggctga ctcagctcaa agacctgatg tactccacca
      841 ggtcgttgat ttcgtcctcg gtcagattct ccgaataggt gaccggcatc accgggtcga
      901 acccggccac cacctgggcc gcagggtcca ccaccgcgct gaacaggtag gcgtcgtcgg
      961 cgagcaccga ctccccggtg accaagggcc ggctcgcccc ggcgagacct ttccaggtgg
     1021 gaccggtgcc gctggccccg tcgatggtgt gacaggccag acacccggtg gcggcggcaa
     1081 tcgcctcccc ccgggccgcg gcctcggagt cgaaggcgat ggcggtggtg gttgcggtgg
     1141 ggtcggcgcc gatcgactcg ccggtgaaca gccacagacc caaacccaga accagcgtca
     1201 acccgagggc gagcgctcct tcagcccagc ttctcataga cggtgaaccg tagacatcga
     1261 ggcggtcaat cctatggttt gagggcgatg accgagctca cgagaggccc gtgatctggc
     1321 agtcacctca gcccctcggc gccgccaggc gtgcgcaccg gaacggcgat cgttagcttt
     1381 ccggcagcag tgaccgctcg agtcgaggag gaaacgtgac cggcatcgat gttgccctct
     1441 tgttgttgag actgtgggcc gggatcgtga tcattgccca cggggtgaac catggccgca
     1501 ctctggaggg aaccgccaaa tggttcgcct ccaagggctt caagtcggcc gggttgaacg
     1561 ccaggatctc cgccctcaac gagatcgcga tcggagtcgc gctgatcgcc gggctgttga
     1621 cctcggtggc ggccgccggt ctggtcgcaa cgatgttcgt cgccttctgg tcgatccatc
     1681 gcttcgtcgg gttcttcaac ttccgccgac ccgatgaggg ctatgagtac gtcgccacgc
     1741 tcgccgtcat cggcactgcc gtggccatcg ccggccccgg gcggtactcg gtcgacgagg
     1801 cgatcggaat cgccggcaac ctggacggct gggtgggagc agccatcgtc ggcgcgggcc
     1861 tgttggtggc ggtcgggcag ctggccacct tctggcgcaa gcccgccggc gacggagggt
     1921 ctgcttgagc ctgacaccgc tccccgatga cttccagttc acccgggacc agctgcatca
     1981 gatcgccttc tatgcgctgg cccctgcccg ctacgcggtg gaggggcgga tgggcctcag
     2041 ggccactccc ggaggttttg ggactccgga gttcgacggg cgtgtcgctc gagtcg
//
LOCUS       DATITW010000701         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_154261, whole genome shotgun sequence.
ACCESSION   DATITW010000701 DATITW010000000
VERSION     DATITW010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154261"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VLS86_09780"
     CDS             complement(<1..182)
                     /locus_tag="VLS86_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSO50822.1"
                     /translation="MDTKTLDSRISDLTASLGDPTRRAIYIAVRESAEPLTTSNVAAL
                     FDIHPNVARHHLDRLA"
     gene            252..1334
                     /locus_tag="VLS86_09785"
     CDS             252..1334
                     /locus_tag="VLS86_09785"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HSO50823.1"
                     /translation="MSSNNEVFQAVASVESPLLPGRTLGDLGLVKGAEKTISGKIIVD
                     LLLPAPNPSPTLNDRLQVALATFGRGADVTVEVMTEPAATEWIGKLRSESGPALGEPG
                     SATRVVAISSGKGGVGKSSVSSNLAVSLRRAGKTIGIVDGDIWGFSIPRMLGIDHPPV
                     LIGESIIPPTAHGVKVMSMDYFVGDDKAVIWRGPMLHRAIEQFLKDVFWDNLDFLIVD
                     MPPGTGDIAISMAQFLPRAQVLLVTTPQPTAQRVARRAALMAAEVDQQVIGVVENMSW
                     FTGDDGTRYELFGSGGGAALAGELGVDLMAQIPLLPAMGRGADQGHPVNVAAAGSEAA
                     EAFDHLAGAVIEKRPRIRTNPALVIN"
     gene            1331..>1649
                     /locus_tag="VLS86_09790"
     CDS             1331..>1649
                     /locus_tag="VLS86_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50824.1"
                     /translation="MSLRTSTRSTLAWAFGICLSILFLSLWGRAVVADTETLGESLAP
                     LGQSDVVGDLLADWMSAELGESAVDPAMTAPTVGLVLGSSTFENTIAGLVDEMVGAAA
                     SSDP"
BASE COUNT          270 a          514 c          556 g          309 t
ORIGIN      
        1 tcggcgagac ggtcgagatg atgacgggcg acattgggat gaatatcgaa cagggcggcc
       61 acgttcgagg tggtgagcgg ctcggcagac tccctgaccg cgatgtagat cgcacgtcgc
      121 gtcgggtcac caagcgacgc ggtgaggtca gagatcctgg agtcgagtgt ctttgtgtcc
      181 atgaggggta ccgacaggtc aaaactatag tttggcgggt cttctatcca ctgtggccgt
      241 aggggaaatc aatgtcttcc aacaacgagg tcttccaagc ggtcgcgtcc gtcgagtccc
      301 cgctgctccc cgggcgcacc ctcggagatc tcgggttggt caagggcgcc gagaagacga
      361 tctcggggaa gatcatagtc gatctgctgc ttccggcgcc aaatccttcc ccgaccctga
      421 atgaccggct tcaggtggcg ctggccacct ttggtcgtgg cgctgatgtc acggtcgagg
      481 tgatgaccga gcctgcggcc accgagtgga tcggaaagct cagatcggag tccggtccgg
      541 ccctcggcga gccgggctcg gcgacgaggg tggtcgccat ctcctcggga aagggcgggg
      601 tgggcaagtc ctctgtcagc tcgaacctcg cggtctccct tcgccgcgcc ggcaagacga
      661 tcggcatcgt cgacggcgac atctgggggt tctcgatccc gaggatgctc ggaatcgacc
      721 atcccccggt gctcatcggg gagtcgatca tcccaccgac cgcccacggg gtgaaggtca
      781 tgtcgatgga ctacttcgtc ggtgacgaca aggcggtcat ctggagaggg ccgatgctcc
      841 atcgcgccat cgagcagttc ttgaaggacg tgttctggga caatctcgac ttcctgatcg
      901 tcgacatgcc gccgggcacg ggagacatcg cgatctcgat ggcccaattc ctgcccaggg
      961 cccaggttct cctggtgacg accccgcagc cgactgcgca gcgcgtggcc cgtcgagcgg
     1021 cgctgatggc ggccgaggtc gaccagcagg tgatcggtgt cgtggagaac atgagctggt
     1081 tcaccggcga cgatggaacg cgctatgagc tgttcggctc gggcggcggg gccgctctgg
     1141 ccggagagct cggggtcgac ctcatggcgc agataccgct gttgccggcc atggggcggg
     1201 gggcagacca gggtcacccg gtgaacgtgg ccgccgccgg ctccgaggcc gccgaggcct
     1261 ttgaccatct ggccggcgcc gtcatcgaaa agcgacctcg aattcggacc aaccccgccc
     1321 tggtgatcaa ttgagtctcc gcacctcgac gcggtccacg ctggcctggg ccttcggcat
     1381 ctgcctctcc atactcttcc tctcgttgtg gggtcgggcg gtggtcgccg acaccgagac
     1441 cctgggtgag tctctcgccc ctctcggtca gtccgacgtg gtcggcgatc tcctcgccga
     1501 ctggatgtcc gccgagctcg gcgagtcggc tgtcgatccg gcgatgacag cccccactgt
     1561 tggcctggtg ctgggatcct cgacattcga gaacaccatc gccggcctcg tcgatgagat
     1621 ggttggtgca gccgcatcgt cagatccag
//
LOCUS       DATITW010000702         1645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_274662, whole genome shotgun sequence.
ACCESSION   DATITW010000702 DATITW010000000
VERSION     DATITW010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274662"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VLS86_09795"
     CDS             <1..217
                     /locus_tag="VLS86_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HSO50825.1"
                     /translation="RSNLRELTAMAPNKATRAKLRLFRTYDPDSDEDEIPDPWGGATE
                     GYEETIRIVRAAARGLVEDLFVRAQAW"
     gene            complement(205..918)
                     /locus_tag="VLS86_09800"
     CDS             complement(205..918)
                     /locus_tag="VLS86_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50826.1"
                     /translation="MNPLPPGLVETRDQLHQLAFFALAAARYKAVGRMGLRATPGGFG
                     TPEFEGRSARIEGDLLVFASEGNIATQPVTTIRSAAQFVGIEYEEVWFPGFGDPLPPA
                     DPDADLALDDAGVEAVADWFEFGYELLNRLRGYGTGDDAVSEVQIWPEHFDAATEMGA
                     EARGQRASYGASPGDTAHPEPYVYVSAWGEIDRSNRFWNDTAFNGASLGYRSLADEVD
                     PVTTALVFLLEGHRILHQA"
     gene            complement(915..1427)
                     /locus_tag="VLS86_09805"
     CDS             complement(915..1427)
                     /locus_tag="VLS86_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSO50827.1"
                     /translation="MNEIDFVLTLLRVWAGIVILAHGINHGRTLAGTADWFAKKGFRS
                     PALNARISAANEIALGLGLIVGLLTTVAAAGLAATMFVAFWSIHRFAGFFNFRRPDEG
                     YEYVTTLALVALAIAILGPGSLSIDSALGIADDLDGWLGAGIVGVGLVVAVLQLAVFW
                     RRPAPSGSVS"
BASE COUNT          320 a          540 c          536 g          249 t
ORIGIN      
        1 tcgctccaac ctgcgcgaac tgaccgcgat ggcgccgaac aaggccacac gggccaaact
       61 tcgactgttt cgcacctacg accccgactc ggacgaggac gagatacccg atccctgggg
      121 aggggccacc gaaggctacg aggagaccat ccgcatcgtc cgggccgcag cccgggggct
      181 ggtcgaggac ctcttcgtcc gggctcaggc ctggtgaagg atccggtgcc cttctagtag
      241 gaagacgagg gcggtcgtca ccggatccac ctcgtccgcg agcgaccgat aaccgaggct
      301 tgccccattg aacgcggtgt cgttccagaa ccgattggac cggtcgatct cgccccacgc
      361 cgacacatag acgtatggct ccggatgggc ggtgtccccg ggcgaggccc cgtaactggc
      421 tcgctggcca cgtgcctccg cccccatctc agtggcggcg tcgaaatgct ccggccagat
      481 ctgcacctcg gataccgcgt catcgccggt cccgtacccc cgcagccggt tcagcaactc
      541 gtacccgaac tcgaaccaat cagccacggc ctcgactccg gcgtcgtcga gggcgagatc
      601 ggcgtcgggg tcggccggtg gcaatgggtc gccaaagccc gggaaccaaa cttcctcgta
      661 ctcgataccg acaaactggg ccgccgaacg gatagtggtg accggttgag tggcgatgtt
      721 gccctccgac gcaaagacca ggaggtcgcc ctcgatccgt gccgatcggc cctcgaactc
      781 tggcgtgccg aatccgccgg gtgtggcacg cagacccatc cggccaacgg ccttgtacct
      841 ggcggcggcc aacgcgaaga atgcaagctg atgaagctgg tcccgggtct cgaccagccc
      901 gggtggaagg ggattcaaga taccgatccg ctcggtgccg gcctgcgcca gaacacggcg
      961 agctggagaa cggcgacgac caggcctacg ccgacgatgc cggccccgag ccagccatcg
     1021 aggtcatctg cgatgcccag cgcagagtcg atggagagcg agcccggtcc caggatcgcg
     1081 atcgccagtg cgaccagggc cagggttgtc acgtactcat agccctcgtc tggccgacgg
     1141 aagttgaaga acccggcgaa gcgatgaatc gaccagaaag ccacgaacat ggtggccgcc
     1201 agaccggcag cggcaacagt ggtgagcagc ccgacaatca gcccgagccc aagagcaatc
     1261 tcattggcag ccgagattcg ggcgttgagg gcgggcgagc ggaacccctt cttggcgaac
     1321 cagtcggcgg ttcccgcgag ggtccggccg tgattgatgc catgggcgag gatcacgatg
     1381 cctgcccaaa cccgaagcaa tgtgagcaca aagtcgatct cgttcatgcc agaaccttac
     1441 gttccgagcg ccgattgtta cggttccgct gacctggaaa tggagacttc tcgatgaacg
     1501 gttgggcaga gggggcactg gccctggcag tgaccctggt cttgggggcc gggctctggt
     1561 tgttcagagg cgagccggtg ggcgacgaga ccacggacac caccttcccg ctcgtggtgg
     1621 acaccgaagc cgcggcccgg gctca
//
LOCUS       DATITW010000703         1869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_314941, whole genome shotgun sequence.
ACCESSION   DATITW010000703 DATITW010000000
VERSION     DATITW010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314941"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VLS86_09810"
     CDS             <1..206
                     /locus_tag="VLS86_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSO50828.1"
                     /translation="LGRSGVKAVSHYVPLHNSPAGRALGREGGSCLVAEDVSDRLIRL
                     PLYPTLTDSEVERVTEAVMSFRP"
     gene            251..988
                     /locus_tag="VLS86_09815"
     CDS             251..988
                     /locus_tag="VLS86_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSO50829.1"
                     /translation="MLSSDVESARFGYRFGRCTIPTTSPATPTDIARAIEDHDAEITV
                     LRYPADRVTWFDHLARELSEHVLIHADTLVYWELEAGSGNPPQAIDGIATTSPIDAEM
                     IGTLTARVFEGYSNHYTANPLLSASAAEAGYREWAMRTPLQDALVLEFDGSPVGIATT
                     DPTAAHIEIMLAGVVPEQRGRGLYQYLLRDVESRARQQALESVVISTQAHNTAVQRAW
                     ARYGFLPLAAFTTLHVIRATTWAPDRD"
     gene            complement(994..>1869)
                     /locus_tag="VLS86_09820"
     CDS             complement(994..>1869)
                     /locus_tag="VLS86_09820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSO50830.1"
                     /translation="ETLQILVERIHGVLGPLGIDYEVVLVVDGSPDDTWEIARRLASH
                     ERVRALNLMRNYGQHNAILAGVLNAENDLIVTMDDDLQHPPEAIPQLIEALTDDLDLV
                     YGVARFEEHGFWRSLASKSVKSAMAMTLGVEHARKISAFRVFRSELRQPFVEMRDAFV
                     SLDVILSWVTTRIASIEIDMDRRTESESNYNVRSLFSHALNMVTGYSTTPLKLVTLLG
                     FATALVGFGLLVFVLVLFFTGQTEVAGFTTIASMVALFAGAQMTAIGILGEYLGRLHF
                     RSMHRPTFVIRDEAK"
BASE COUNT          356 a          600 c          574 g          339 t
ORIGIN      
        1 atcttggccg aagcggagtc aaggcggtca gccactatgt cccgttgcac aactcccctg
       61 ccggccgggc attggggaga gagggcggct cttgtctcgt cgccgaggat gtgtcggacc
      121 gcctgattcg gcttccgctg tatcccaccc tcaccgattc ggaggtggag cgggtgacgg
      181 aggcggtcat gagctttcgg ccctgagcac agtggcgctc tccgatctca ccgcggacca
      241 tcgactggag gtgttgtcca gcgatgtgga gtcggcccgt ttcgggtatc ggttcgggcg
      301 gtgcacgatc ccgaccacgt caccggccac gcccaccgac atcgcccgag cgatcgagga
      361 tcatgacgcc gagatcaccg ttctccgcta tccggccgac cgggtgacct ggttcgacca
      421 cctggcacgg gaactgagcg agcatgtgct gatccacgcc gacaccctgg tctactggga
      481 gttggaagcc ggctcgggga acccaccgca agctatcgat ggcatcgcca ccacgagccc
      541 gatcgacgcc gagatgatcg gcactctgac cgccagggtg ttcgaggggt actcgaacca
      601 ctacacggca aacccactcc tgtctgcgtc ggcggcggag gccgggtacc gggagtgggc
      661 gatgcgaacg cccctgcagg acgcattggt gctcgaattc gacggttcac cggtcgggat
      721 tgcgaccacc gaccccacgg cagcgcacat cgagatcatg ttggctggtg tcgttcccga
      781 acaacggggc aggggcctgt accaatatct gctgcgggat gtcgagtccc gcgccaggca
      841 acaggcactg gaatcagtgg tgatctcgac ccaggcgcac aacacagccg tgcagcgggc
      901 gtgggcgcgc tacgggttcc tccccctcgc cgccttcacc accctccatg tcatccgggc
      961 aacgacctgg gccccggatc gagactgagg cgtctacttg gcctcgtccc tgatcacgaa
     1021 cgtcggccga tgcatgctcc gaaaatgaag acgccccagg tattcaccga ggattccgat
     1081 cgctgtcatc tgggcgcctg cgaacagagc caccatcgag gcgatggtgg tgaatcccgc
     1141 cacttccgtt tgaccggtga agaagagaac cagcacgaac accaggagcc cgaacccgac
     1201 cagtgcggtg gcgaacccga gcaacgtcac caacttcaac ggtgtggtgc tgtagcccgt
     1261 caccatgttc aacgcatggc tgaacaggga cctgacgttg tagttcgatt cactctccgt
     1321 tcgccggtcc atgtcgatct cgatcgatgc gatccgggtg gtgacccagg agagaatcac
     1381 gtccaatgac acgaacgcgt cccgcatctc cacgaatggc tgcctgagct cactgcgaaa
     1441 caccctgaag gcgctgatct tgcgtgcgtg ctcgacgccg agagtcatcg ccatcgcact
     1501 cttcacggac ttcgaggcca gacttcgcca gaaaccgtgc tcctcgaacc tggcgactcc
     1561 gtacaccaga tcgagatcgt cggtgagagc ctcgatcagt tgggggatgg cctccggagg
     1621 gtgctggagg tcgtcatcca tggtgacgat cagatcgttc tcggcattca ggaccccggc
     1681 cagaatcgcg ttgtgctggc cgtagttccg catcaagttg agggcccgaa cccgctcgtg
     1741 agacgcgagt cggcgtgcga tctcccaggt gtcgtcgggg ctgccgtcga ccacgagaac
     1801 gacctcatag tcgattccga ggggaccgag tactccatga attctctcga cgagaatctg
     1861 cagcgtctc
//
LOCUS       DATITW010000704         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_715737, whole genome shotgun sequence.
ACCESSION   DATITW010000704 DATITW010000000
VERSION     DATITW010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715737"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1068)
                     /gene="ftsY"
                     /locus_tag="VLS86_09825"
     CDS             complement(70..1068)
                     /gene="ftsY"
                     /locus_tag="VLS86_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HSO50831.1"
                     /translation="MDTSTVLIGVAVVILLLVGVIWWVRRSSKQETVTSLRPLDIRPS
                     RLGRSAVVLGAALRRAWGEGLGESTWSELEEALLGADVGVEATSSVVAGVKAANPETP
                     ADARLELARQLRAALGDGDRDMHLEGSPAVILVVGVNGTGKTTTIAKLAERLKGEHKS
                     VILAAGDTFRAGAAAQLETWGDRLGLVVVSGESGGDPASVAFDAIGAARARGTDVVIV
                     DTAGRLHGKKNLMSELGKIHKVAAGDRGQVDEVLLVLDATSGQNGVTQVREFTAAVPV
                     TGIVLTKLDGTAKGGIVIAVERDLGVPVKFVGLGEGIDDLVPFDPDEFVTDLLEQA"
     gene            complement(1090..>2121)
                     /locus_tag="VLS86_09830"
     CDS             complement(1090..>2121)
                     /locus_tag="VLS86_09830"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSO50832.1"
                     /translation="EAELGRVGDPDQPGAGVGRLEVVEQCSRRAVEVLHRRLTELRER
                     QAGLRSTNEVTLAELARTRAEFEERRDAIGSRRQRLGELQVRLTELRLMRESVIEGIR
                     RDADAEADAALGAVRPDYPPDADLSQILEERTARLRRLGPINPLAAAEHAELDERHRF
                     LAEQLADVESSRSELRKVIRALEEEIEQRFQEAFTEVAEAYQRYFSILFPGGRGRIRP
                     TDHEDPHAGLVIEAQPLGKKVSQLSLLSGGERSLAALAFLFSIFEARPSPFYVLDEVE
                     AALDDANLRRFLRIVDEFRQRAQLLIVTHQQQTMEAADVLYGVTMEPGGSSQVVRKDM
                     SSVGIDLSA"
BASE COUNT          357 a          747 c          659 g          358 t
ORIGIN      
        1 ggaagttcga gtagggggag tacgagttct cggcggcagc ccgcgctgca gcgatcagtt
       61 catctcgagt cacgcttgct ccaggagatc ggtgacgaat tcatccgggt cgaaaggcac
      121 gaggtcgtcg atcccctctc ccaggcccac gaactttacc ggcacaccga gatcacgctc
      181 cactgcgatc acgatcccgc ctttggcggt tccgtcgagc ttggtgagca cgatcccggt
      241 gaccgggacc gcggcggtga actctcgcac ctgagtcacc ccgttctgcc cactggtggc
      301 gtcgaggacg agcagcacct cgtcgacttg accacggtcg ccggcggcga ccttgtggat
      361 cttgcccagc tcggacatga ggttcttctt gccatggaga cgccctgcgg tgtcgacgat
      421 gacgacgtcg gtgcctctcg cccgagccgc cccgatcgcg tcgaaggcca ccgaagccgg
      481 gtccccaccc gactccccgg aaacgaccac caggcccagc cggtcgcccc atgtctcgag
      541 ttgggccgca gccccggccc ggaaggtgtc gccggcggcc aggatcaccg acttgtgctc
      601 gcccttgagt cgctcggcca gtttggcaat ggtcgtagtc ttcccggttc cgttcacacc
      661 gaccaccagg atcacagccg gtgatccctc caaatgcatg tcgcgatctc cgtcgcccag
      721 cgcggcgcgc agctgtcgcg ccagctcgag acgagcgtcg gcgggtgtct cgggattggc
      781 ggccttgact ccggcaacca ccgacgaagt ggcctcgacg ccgacatcgg cgccgagcag
      841 cgcctcctcc aactcgctcc aggtgctttc accgagacct tctccccacg cccggcgcag
      901 ggcggcgccc aacaccacgg cgctgcggcc gaggcgggag ggtcggatat cgagcgggcg
      961 caggctggtg acggtctcct gcttcgagct gcgacgcacc caccagatga ccccgaccag
     1021 aagcaggatc acgacggcga cgccgatcaa gactgtggaa gtgtccattg agtcgatcaa
     1081 gctaacggtt caggcagaaa ggtcgatgcc gacgctgctc atatccttgc gaactacctg
     1141 agacgagccc ccgggctcca tcgtgacccc gtacaggaca tcggccgctt ccatggtctg
     1201 ttgctggtgg gtcacgatca acaactgggc cctttgcctg aactcgtcga cgatccggag
     1261 aaatcggcgc aggttggcgt cgtccagagc ggcttcgacc tcgtcgagca catagaacgg
     1321 gctgggtcgc gcctcgaaga tggaaaacag gaaagcgagg gcggcgagac tccgctcacc
     1381 tcccgagagg aggctcagct ggctcacctt cttgcccagc ggctgggcct cgatcaccaa
     1441 gccggcatgc gggtcttcgt ggtcggtcgg tcggatccgc ccccgcccac cgggaaacag
     1501 aatcgagaaa tagcgctggt aggcctcggc cacctcggtg aatgcctcct ggaaccgctg
     1561 ttcgatctcc tcttccaggg ccctgatcac cttgcgcaac tccgagcgcg acgactccac
     1621 gtcggcgagc tgctcggcga ggaacctgtg acgctcatcc agctcggcat gctccgcggc
     1681 ggcaagcggg ttgatcgggc cgagacgtcg gagccgggct gtcctctcct cgaggatctg
     1741 actcaggtcg gcatccggcg ggtagtcggg ccggaccgct cctagggcgg catcggcctc
     1801 ggcatcggcg tcacgtctga tcccctcgat cacactctcc cgcatcagac gcagctcggt
     1861 gagccgcacc tgaagctctc cgagccgctg tcgtcggcta ccgatggcgt cacgtcgctc
     1921 ttcgaactcg gcgcgcgtcc tcgccagctc ggccaatgtg acctcattgg tcgaacgcaa
     1981 ccccgcctga cgctcccgga gctcggtcaa acgccggtga aggacctcga ccgcccgtcg
     2041 tgagcactgc tcgaccactt cgagccgccc gacaccggcg ccgggctggt ccgggtcgcc
     2101 gacacggccg agctcggcct c
//
LOCUS       DATITW010000705         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_154350, whole genome shotgun sequence.
ACCESSION   DATITW010000705 DATITW010000000
VERSION     DATITW010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154350"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VLS86_09835"
     CDS             complement(<1..1026)
                     /locus_tag="VLS86_09835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSO50833.1"
                     /translation="MSARPSSLHPFSRFSLAAVTATAMGVATFTQSAFGVLAADLIAE
                     FDVARWQIGFLVTASGFTGAFLSPTFGRLTDRIGSVRSMVGVFIFGALYMSVVFTAPN
                     YVILVLGALLAGVPNGWTNPATNALIVDNVAAGTRGVITGIKQSGVHMGTTLGGFFLP
                     MISAIAGWRAAFASFLVFPVAGLIGMWRRPQVERAPRPRGEKQGKVPTAIRWVALYGA
                     LSGLGVSATITFLPLFANEDQGWSTARAGLLIAGIGLVGVISRIVWGSASERWLGHGR
                     TLRLMALQSMVAAVLLSLASVELAPSWVLVPAALLLGSGAIAWNAVGMLAVMDFSPSH
                     MVGRGTGL"
     gene            1058..2092
                     /locus_tag="VLS86_09840"
     CDS             1058..2092
                     /locus_tag="VLS86_09840"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HSO50834.1"
                     /translation="MKRIVVGTEKGGYLLTRSNGSWTVDGPMFPGWKVTAFGRAPDGA
                     AMAAVGSNWFGVGIHVSEDWRNWKQTESPPSWPEQTGRKMEQIWALHTEGGRLWAGVA
                     QAGLFTSDDGGGSWDPVDGLNEHPTRDDWHPGFGGLCAHRILTAGEQIWVGISAVGVF
                     RSDDGGATWVAKNLGVPPVDTAEGAAPPEVGYCVHGLAQDPADPRRLWRQDHRGVFRS
                     TDGGDHWERIENGLPAGFGFVMWRHPGSGRLLTVPLTSDENRVPVDGVLRAYVSDDDG
                     DTWQVAGRGWQVAPQFTGTLRGAFDGDDEGMFCFGTTGGKLWLTLDNAMSWQELEPAF
                     PRIAAVRIEE"
BASE COUNT          422 a          710 c          774 g          346 t
ORIGIN      
        1 caacccggtt cccctgccca ccatgtgtga aggggagaag tccatcacgg ccagcatccc
       61 caccgcgttc caggcgatgg cgcccgagcc gaggagcagc gctgcgggga ccaacaccca
      121 actcggcgcc aactcgactg aggcgaggct gaggaggacc gcggcgacca tcgattgcaa
      181 tgccatgagt cgcagggtgc gaccgtggcc cagccatcgt tccgaagccg agccccacac
      241 gatccgggag atcacgccga ccaggccgat gccggcgatg aggaggccgg cgcgggcagt
      301 gctccagccc tggtcttcat tggcaaacag cgggaggaac gtgatcgtgg cggagacgcc
      361 gagcccgctc agcgcgccgt aaagcgcgac ccatctgatc gcggttggca cctttccctg
      421 tttctcgccg cgaggcctcg gggctcgctc gacctgggga cgacgccaca tcccgatcag
      481 acccgcgacg gggaacacga gaaatgaggc aaacgcggcg cgccagccgg caatcgccga
      541 gatcatcggg agaaagaaac caccgagcgt ggtgcccata tgaacacctg actgcttgat
      601 cccggtgatc acaccccggg tgccggctgc gacgttgtcg acgatgaggg cgttggtggc
      661 gggattggtc cacccattgg gcacaccggc aagcaatgcc ccgagcacca agatgacata
      721 gttgggcgcc gtgaacacca cgctcatgta gagcgctccg aatatgaaca cccccaccat
      781 ggatcggacg cttccgatgc gatcggtcag ccggccgaac gttggtgaga gaaacgcgcc
      841 ggtgaaccca ctggcagtca ccaggaaccc gatctgccat cgcgcgacat cgaactccgc
      901 gatgagatcg gctgcgagca ccccaaatgc gctctgggtg aaggtggcga cccccatcgc
      961 cgtcgccgtg accgcggcca gggagaaccg actaaatgga tgcagcgatg acggtcttgc
     1021 tgacatgtag attcggattg tcgatcggag cagccgtgtg aagcgaatcg tggtgggcac
     1081 ggagaagggc ggttaccttc tcacccggtc caatgggtca tggacggtag acggcccgat
     1141 gttccccggg tggaaggtga cggcatttgg ccgcgctcct gacggggcgg cgatggctgc
     1201 ggtcggcagc aactggttcg gtgtcggcat ccatgtcagc gaggactgga ggaattggaa
     1261 gcagaccgag tcgcccccat cctggccgga gcagacaggc cgcaagatgg agcagatctg
     1321 ggccttgcac accgagggag ggcgcttgtg ggcgggcgtc gcccaggccg ggttgttcac
     1381 atcggacgac gggggcggga gctgggaccc ggtcgacggg ctcaacgagc acccgacccg
     1441 cgacgactgg catccgggtt ttggcgggct ctgcgcccac cggatcctga ccgcgggtga
     1501 gcagatatgg gttgggatat cagcggtggg tgtgttcaga tccgacgacg gcggggcaac
     1561 ctgggtggcg aagaacctcg gtgtgccgcc ggtcgacacc gccgaaggcg ccgccccgcc
     1621 cgaggtgggt tactgcgtcc atggactggc acaggatcct gcggacccgc gacgcttgtg
     1681 gcgtcaggat caccgaggtg ttttccgatc gacggacggt ggcgatcact gggagcggat
     1741 cgagaacggt ctccccgcag gattcggatt cgtgatgtgg cgccatcccg gctcgggccg
     1801 cctcctcacc gtgccactca cctccgacga gaaccgggtc ccggtcgacg gcgtgctccg
     1861 ggcctacgtc tccgacgacg acggcgacac atggcaagtc gctggacgtg gttggcaggt
     1921 agccccacag ttcaccggca ccctccgcgg tgccttcgat ggcgacgacg aagggatgtt
     1981 ctgcttcggc accactggcg gcaagctctg gttgacgctc gacaacgcga tgtcgtggca
     2041 ggagttggag ccggcgttcc cgcggatcgc cgcggtgcgg atcgaagagt gagtgccagc
     2101 gtccgactgc cgcgggtcct gtctcaggct gtcggcatag ggcccaggca cgaggcagag
     2161 ggccacaacc tgggcgaggt cctcggtgac ctcttcgccc ttcacccggg tctgaagaac
     2221 cacctcctcg acgagcaggg acgaatcaga cc
//
LOCUS       DATITW010000706         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_394722, whole genome shotgun sequence.
ACCESSION   DATITW010000706 DATITW010000000
VERSION     DATITW010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394722"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VLS86_09845"
     CDS             <1..396
                     /locus_tag="VLS86_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSO50835.1"
                     /translation="SRMLRREQDRAMELLSFVGIDKLASLPARSLSYGQKKLLEFASV
                     LMPDPKLILLDEPAGGVNPALLERIMDHIRELNRSGITFLIVEHNMDVVMSLCNSVIV
                     MAHGEVLAEGSPEAIQADDIVLDAYLGRV"
     gene            397..1134
                     /locus_tag="VLS86_09850"
     CDS             397..1134
                     /locus_tag="VLS86_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50836.1"
                     /translation="MALIEVHDVVAGYGAGPDILTGVSVTIEERTSYCIVGPNGAGKS
                     TLLRAIAGILEPRSGEVLLSGTVVNGLRPDQMLALGVCFVPQDPTLFPAMTVRENLLM
                     GGYLIRDRKLLGERLDLVYENFPALAGRESQNAGTLSGGEQQMVALGRSLMLEPKVLM
                     IDEPSLGLAPMIAELIFEKVREFASLGITVVLVEQNAVRGLESSDWGIVLDLGSKECE
                     GPATGILEDPRIRELYLGKRLARKNLQ"
     gene            1131..2006
                     /locus_tag="VLS86_09855"
     CDS             1131..2006
                     /locus_tag="VLS86_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSO50837.1"
                     /translation="MTNFAQTLILGLLLGGVYALMASGLTLLFGVMRIVNLAHAALIL
                     VAAYLAFVLFEATSLDPIVSLVITMPVLFGLGVGIYLLIFPRIEGTHRYVEMTVLLTF
                     GLALIVEGLLTFAFTGIFRSTTPDYVLDSIAVGDLFIPTAQLYASIVCAVLLFALWAF
                     LQLTRTGRAIRATTQNRAAAQIVGINVRRTSALAFGIGLSLAGASGSLISFLFSFFPA
                     KHWQWIALMLALIVLGGMGSIKGAVIGALGLAVASAFVSDSFGPTWSPITFYLALFLI
                     LLVRPQGLYGTKLRL"
     gene            2006..>2370
                     /locus_tag="VLS86_09860"
     CDS             2006..>2370
                     /locus_tag="VLS86_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50838.1"
                     /translation="MTNIPIIEKSGEGDLTPTSDGRPKGVMSRRVTLVVLLAMVLLYP
                     FIARSIFSSYNYVMQLGIVVLMWIAMSSSWNIIGGFAGYISLGHAVFYAIGGYVSGMM
                     LIHWGVSPFLTAPIAGLAAV"
BASE COUNT          362 a          762 c          742 g          504 t
ORIGIN      
        1 agccggatgc tgcgtcgtga gcaggaccgc gccatggaac tgctttcgtt cgtcggcatc
       61 gacaagttgg cgtcgctgcc ggcgagatcg ctgtcctacg gtcagaagaa gctgctcgag
      121 ttcgcctcgg tgctgatgcc cgatcccaag ctcatccttc tcgacgagcc cgccggtggg
      181 gtcaaccccg ctcttctgga gcggataatg gaccacatcc gggagctgaa ccggtctggc
      241 atcacctttc tgatcgtgga gcacaacatg gacgtggtga tgagcctgtg caactcggtc
      301 atcgtcatgg cccacggtga ggtcttggcc gagggctccc ctgaggccat ccaggcggac
      361 gacatcgtcc tcgatgcgta cctggggagg gtgtaggtgg cgttgatcga agtccacgac
      421 gtcgtcgccg gctatggcgc cgggccggac atcctcaccg gggtcagtgt caccatcgag
      481 gaacgcacct cgtactgcat cgtcggcccc aacggcgcgg gcaaatccac cctcctccgc
      541 gccatcgccg ggatcctcga gccccggtcc ggtgaggtgc tcctgagcgg aacggtcgtg
      601 aacgggcttc ggcccgacca gatgttggcc ctgggcgtct gcttcgtccc tcaggacccc
      661 acgctgttcc cggcgatgac agtccgcgag aacttgctga tgggtggata tctgatccgc
      721 gaccgaaagc tcctgggcga gcgtctcgat ctcgtctacg agaacttccc cgcgctggct
      781 ggccgggaga gccagaacgc cgggacgttg tcgggtggcg agcagcagat ggtcgcactg
      841 ggtcggtcac tgatgctcga gcccaaggtg ctgatgatcg acgagcccag tctcggtctt
      901 gccccgatga tcgccgaatt gatcttcgag aaggtccggg agttcgcgtc tctggggatc
      961 acggtggtcc tggtcgagca gaacgcggtg cgggggctgg agagctcgga ctggggaata
     1021 gtgctcgacc tcggatcgaa agagtgcgag ggcccggcca ccggcattct cgaggatccg
     1081 cggatccgcg agctctatct cggcaagcgg ctggcgcgca agaacctgca atgacgaact
     1141 tcgctcagac actcatcctc ggtttgttgc tgggcggggt ctacgccctg atggcgtccg
     1201 ggctcaccct cctcttcggg gtgatgcgca tcgtcaacct cgctcacgcc gctctgatct
     1261 tggtagccgc ctacctcgcc ttcgttctct tcgaagcgac gtccctcgat ccgatcgtgt
     1321 cgctggtgat caccatgcct gtgttgttcg ggctcggtgt gggcatctat ctgcttattt
     1381 tcccgcggat cgaggggaca caccggtacg tggagatgac tgtgctgctc acgttcggcc
     1441 tggccctgat cgtggaaggg ctgctcacct ttgccttcac cgggatcttc aggtcgacca
     1501 cgccggatta cgtgctggat tccatcgccg tcggggacct cttcatcccc accgcccagc
     1561 tctacgcgtc gatcgtctgt gcggtcctgc tcttcgcgct gtgggcattc ctccaattga
     1621 cacgcaccgg tcgggcgata cgtgccacga cgcagaaccg cgccgcggcc cagatcgtcg
     1681 ggatcaacgt gcgcaggacc tcggcactgg ccttcggcat cgggttgtcg ttggccgggg
     1741 cttcggggtc tctgatcagc ttcctcttct ccttcttccc ggccaagcac tggcagtgga
     1801 tcgcgttgat gctggccctc atcgtcctcg gcgggatggg gagcatcaag ggcgcggtga
     1861 tcggggcgct gggcctcgcc gtcgccagcg ccttcgtcag cgacagcttc ggccccacct
     1921 ggtcgccgat caccttctac ctcgccctgt ttctcatcct cctcgtgcgg ccccaaggtc
     1981 tgtacgggac caagctgagg ctctgatgac caacatccca atcatcgaga aatcgggtga
     2041 aggagacctg actccgacca gcgatgggcg accgaagggg gtgatgtcgc gccgggtcac
     2101 gctcgttgtg ctgctggcga tggtcctcct ctacccgttc atcgcccgct ccatcttctc
     2161 ttcgtacaac tacgtcatgc agctcgggat cgtggtcctg atgtggatcg ccatgtcctc
     2221 gagttggaac atcatcggcg ggtttgccgg ctacatctca ctgggccacg ccgtgttcta
     2281 cgccattggc ggatacgtgt ccgggatgat gctcatccat tggggggtat cccctttcct
     2341 caccgctccc atcgccggac tggccgcggt
//
LOCUS       DATITW010000707         1851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_34764, whole genome shotgun sequence.
ACCESSION   DATITW010000707 DATITW010000000
VERSION     DATITW010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34764"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VLS86_09865"
     CDS             complement(<1..334)
                     /locus_tag="VLS86_09865"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HSO50839.1"
                     /translation="MPPVTWTKVAQDGRARAGWLDTPHGRVMTPAFMPVGTRASVKTV
                     DNDDLERIGVEIILANTYHLMLRPGAGLIAKLGGLHGFTSWQSPILTDSGGFQIFSLG
                     PEIDEGGAR"
     gene            complement(342..1355)
                     /gene="queA"
                     /locus_tag="VLS86_09870"
     CDS             complement(342..1355)
                     /gene="queA"
                     /locus_tag="VLS86_09870"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HSO50840.1"
                     /translation="MRVEEFEYPLPESAIAQEPIEPRHDSRLLDTRDLSDHRFIDLPL
                     LLRAGDLVVVNETRVRAARLLGRRKGSGGAVELLVLAARPDGLWEALVRPARRLRPGV
                     ELVFDGLTAEIVEGPGEGLVMVDLRAPDVEAAIETAGEVPLPPYFKGRLDDASRYQTM
                     FARTMGSAAAPTAGLHFTPEVVNGLGARGIAITSVDLHVGLGTFRPMTVEEVESHRMH
                     HEWCSVSEVAAESIAEVRRNGGRVVAIGTTVVRTLETRGRPDGTVEPGESDTDLFLMP
                     GSEFNVVDALVTNFHVPGSTLVVLVAAFMGREWRRAYEVALERGYRFLSFGDAMYAEW
                     QGT"
     gene            complement(1403..>1851)
                     /locus_tag="VLS86_09875"
     CDS             complement(1403..>1851)
                     /locus_tag="VLS86_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010838837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HSO50841.1"
                     /translation="RRSAGIIDVEISDDAAWIIAGRRRSTPRIANRLLARVRDFAVAR
                     AAGVIDPEVADEALRVFEVDDLGLDKVDRSILEAIVGGFGGGPVGLSTLAIAVGEEPE
                     TVEDAYEPFLLQSGLIKRTPRGRMATSRAYTHLGIEQPPDPQGTLL"
BASE COUNT          310 a          656 c          580 g          305 t
ORIGIN      
        1 acctggctcc tccctcgtcg atctcaggcc ccagagagaa gatctggaac ccaccggagt
       61 cagtgaggat cgggctctgc cacgaggtga atccgtgcag gcccccgagc ttggcgatca
      121 ggccggcccc cggacgcagc atcaggtgat aggtgttggc gaggatgatc tccacgccaa
      181 tcctctccag gtcgtcgttg tccaccgtct tgaccgaggc cctggtgccg accggcatga
      241 acgccggggt catcacccgc ccgtgcggcg tgtccagcca accggctcgt gcccggccgt
      301 cttgggcgac cttggtccag gtgacagggg gcatcgtggg attatgtccc ctgccactcg
      361 gcatacatcg cgtcgccaaa ggacaagaag cgataacccc gctccagtgc cacctcgtat
      421 gccctgcgcc attccctccc catgaatgcc gcgaccagca caaccagcgt ggagccgggg
      481 acgtggaagt tggtgaccag ggcgtcgacg acgttgaact ccgatccggg catcaggaac
      541 aggtcggtgt cgctctcccc cggctcgacc gtcccgtcgg gcctgccccg cgtctccagg
      601 gttctcacca cggtggtccc gatcgccacc acccgccccc cattcctccg gacctccgca
      661 atcgactcgg ctgcgacctc tgagaccgag caccattcgt ggtgcatccg atgcgattcg
      721 acctcctcga ccgtcatggg tcgaaaggtg ccgagcccga cgtgaaggtc gactgaggtg
      781 atcgcgatcc ctctggcccc caaaccgttg accacctccg gagtgaagtg gagcccggcg
      841 gtgggggcgg cggccgagcc catggtcctg gcgaacatgg tttggtacct ggaggcatcg
      901 tcgagccgcc ccttgaaata gggggggagc ggaacctcgc ctgcggtctc gattgccgcc
      961 tccacgtccg gggcccggag atcgaccatc accaaccctt caccgggccc ttcgacgatc
     1021 tcggcggtca gcccgtcgaa cacgagctcg acaccggggc gcagcctcct ggcgggcctg
     1081 accagcgcct cccagagacc gtcaggtctg gctgcgagaa ccagcagctc gacggcgccg
     1141 ccgcttccct tcctccgccc cagcagacgc gccgcgcgca cccgggtctc gttgaccacc
     1201 accagatccc ccgcccgcag cagcagtggc aggtcgatga agcgatgatc gctcaggtcg
     1261 cgagtgtcga gcagcctcga gtcgtgtctc ggctcgatcg gctcttgggc aatcgccgat
     1321 tccggcagtg gatattcgaa ctcctcgacc cgcatcgaac gtggaggcta tcgacaggac
     1381 ctctgccgac cagtcggcag agtcagagca gggtcccctg cgggtcggga ggctgttcga
     1441 tgccgagatg ggtgtaggcc cgggaggttg ccatccgacc tctcggcgtc cgcttgatca
     1501 acccggattg gagcaagaaa ggctcatagg catcctcgac ggtctccggc tcctcgccta
     1561 ccgcgatggc cagtgtcgag agcccgaccg ggcctccccc gaatcccccg acaatggctt
     1621 cgagaatcga tctgtccacc ttgtccagac cgaggtcgtc gacttcgaac accctgagcg
     1681 cctcgtcggc gacctcgggg tcgatgacgc ccgcggcgcg ggccacggcg aagtccctca
     1741 cccgagccag gagccggttg gcgatgcgtg gcgtggaccg acggcgtccg gcgatgatcc
     1801 acgccgcgtc gtcggagatc tcgacgtcga tgatcccggc cgagcgacgg a
//
LOCUS       DATITW010000708         1543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_514856, whole genome shotgun sequence.
ACCESSION   DATITW010000708 DATITW010000000
VERSION     DATITW010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514856"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VLS86_09880"
     CDS             complement(<1..805)
                     /locus_tag="VLS86_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50842.1"
                     /translation="MTDLLTQLRDYGRQVESDPMLHAPTPLEEDVLTVPRGTRRISRS
                     WVRGLIAAAMVLLVFLPLMLGRFSEEPTATSTPTTETNIGWVAFAADDAPAGDLDIFM
                     VRPGVEPLRLIGSQGDALDQFCPSFSPDGSKLAYLEADHSGLVPGELGGSAQIVVVGL
                     DETAVSGELLRATATWRVCPTWSPDSTQIAYVTENWELTIGTVGGGVTPLYPMNAGHT
                     GIEWSPDGRVIAVWSFVDRSAPTTAIRLVPVDGSEMKVLATLSESMPELV"
     gene            complement(802..1272)
                     /locus_tag="VLS86_09885"
     CDS             complement(802..1272)
                     /locus_tag="VLS86_09885"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HSO50843.1"
                     /translation="MHQKVPISDAFTEFAQVAEPRLRLALGAAFGFDMAQEATAEALA
                     FAWEHWDRVQATSNPIGYVFGVGRNRARRSLNRQRVPFLPPVRVSEIPWVEPGLPGAL
                     GRLSERQREVIMLLHCFEWSFSEVSEVLGMARGTVQAHERRGMARLRKDLGVEL"
BASE COUNT          317 a          506 c          448 g          272 t
ORIGIN      
        1 cgactagttc aggcatcgat tcggagagtg tggcgagcac cttcatttcg gacccgtcta
       61 ccggaacaag ccggatcgcg gtggttggtg ccgaacggtc cacaaagctc cacacagcaa
      121 tgacacgtcc gtccggggac cactcaatac cggtatgccc agcattcatg gggtagagag
      181 gcgtaacacc gcccccgacc gtcccgatgg tcagttccca gttctctgtc acataagcga
      241 tttgagtgct gtcgggcgac caggtggggc atacgcgcca ggttgccgtc gcccggagaa
      301 gttctccgga tacggccgtt tcgtcgagcc caacaacgac aatctgggcg gaccctccaa
      361 gttcacccgg gacgaggcca ctgtgatccg cctccagata ggccagcttc gagccatctg
      421 gtgaaaacga tgggcagaac tggtcgagcg cgtcaccctg ggatccgatg aggcgaagcg
      481 gctcgacacc aggacggacc atgaagatgt cgaggtctcc agctggtgca tcgtctgcgg
      541 cgaaggccac ccaaccaatg ttggtctcgg ttgtgggagt cgatgtcgcc gttggttcct
      601 cgctgaatcg gcccagcatc agaggcagga agaccaacag gaccatcgca gctgcgatca
      661 agcccctgac ccacgatctc gaaattcggc gtgtcccccg tggcaccgta agaacgtcct
      721 cttccagcgg cgtcggggcg tggagcatcg gatcggattc gacctgtcgt ccgtaatcac
      781 gaagctgggt caacagatcg gtcacagctc cactcccaga tccttgcgga gcctggccat
      841 tccccgccgc tcatgagcct gcacggttcc tctcgccatt cccagcacct cggaaacctc
      901 gctaaagctc cattcgaagc agtgaagcag catgatcacc tcccgctgac gttccgacag
      961 ccgcccgagc gctcccggta ggccgggctc gacccacgga atctcagaga cacggaccgg
     1021 tggcagaaag gggacgcgct gtcggttcag gctccgtcgg gccctattgc gcccgacacc
     1081 gaacacataa ccaatcgggt tggaagtcgc ctgcacccga tcccagtgct cccaggcgaa
     1141 ggccaacgcc tcggcggtcg cctcctgagc catgtcgaac ccgaacgcag caccaagcgc
     1201 caaccgcagc cgaggttcag cgacttgggc gaactcagtg aaggcgtcgg atatcgggac
     1261 cttctggtgc atgttcaccg gatccaagtc tgtcgacgag tccagatgcg tgacaacatc
     1321 attcggaatc gtctgccccg cccgcatcgc ccgaggctcg cgatgtgccg gtctcatgac
     1381 tcggtcaggt gtcgaccatg tcacgtaaca cgccgacgat cgtgtagacc tgatccaagc
     1441 tcgcatttgg cgcctgcacc ccaactacga gacccagttc tgggaaaacc acgagtgccg
     1501 tggcgctcgg tggtccaaga ccgccatggc cgatagccgg cct
//
LOCUS       DATITW010000709         1646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_235152, whole genome shotgun sequence.
ACCESSION   DATITW010000709 DATITW010000000
VERSION     DATITW010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235152"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VLS86_09890"
     CDS             <1..506
                     /locus_tag="VLS86_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50844.1"
                     /translation="AATERGARILAEMLGGASTADAHHITAPHPSGDGAERAIRLALA
                     DSGLEASDVSYINAHGTGTELNDRTEGMVIERIFGDTQPAGSSIKGTTGHGLGASGSI
                     EAVAVVEAIRRRELPPNIGIVNQDPEIPLTDIVREPRDWEPGPVLSNSFGFGGHNTVL
                     AFAPYAD"
     gene            779..>1646
                     /locus_tag="VLS86_09895"
     CDS             779..>1646
                     /locus_tag="VLS86_09895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSO50845.1"
                     /translation="MTFEPRLPTIGFLSTYPPTSCGLATFTHALRTAIADERGFDDGL
                     GVISLVDFAESEAGPDVVYSHLNGDPASVIRTINTLNRFDVVLIQHEYGIYVGPDGSE
                     VLDILAGLHVPSVVTLHTVLSRPSDSQRSILENVVALADQTIVMSDTASQRLIENYDV
                     DPAKITTVPHGARISLSGPSLADGHRPTFLTWGLIGPGKGLETAIDAFAALKDVDPLP
                     RYIILGKTHPKVQAASGDAYLNGLQTRVEELGLQDVVEFDGRYLDIDALTLEVRSADF
                     VVLPYESTEQVTS"
BASE COUNT          316 a          562 c          484 g          284 t
ORIGIN      
        1 aagcggcaac ggagcgaggg gcgaggatcc tcgccgagat gctcggtggt gcctccacgg
       61 ccgatgccca ccacatcacc gccccccatc cgtccggtga tggcgcagag cgtgccatcc
      121 gtctcgcctt ggccgactcc ggtctcgagg caagcgacgt gtcctacatc aatgcccacg
      181 gcaccggaac cgagctgaac gaccgcaccg agggaatggt catcgagcgg atcttcggcg
      241 acacccagcc ggctgggtct tcgatcaagg gcaccaccgg tcatggcctg ggggcttcgg
      301 gctcgatcga ggcggttgca gtggtggagg cgatccgccg acgcgagctc ccgcccaaca
      361 tcgggatcgt caaccaggac cccgagatcc cgctcaccga catcgtgcgc gaaccgcggg
      421 actgggaacc gggaccggtg ctctccaact cattcggttt cggtgggcac aacacggtgc
      481 tcgccttcgc tccctacgcc gactgatcgt tcccccgccc cgtacttggg accaggaggg
      541 ttcatatacg gccctgcgca acccaattac cagggggcaa ctcaggtccg tccgagcgcc
      601 aatctcgggt atggagtacc ccggatccgg gtatagaatg ggtgctacac ggtcgcacaa
      661 cgcgacacac gttcgtaacg cggagttcac aagaactcgc atctccgctg attgagtgac
      721 cgccaggcaa ccacccgggg cggccacccc ggaagtgttt cccgagcgga ggatgacgat
      781 gacatttgaa ccaaggctcc ccacgatcgg gtttctgagc acctacccgc ccacctcttg
      841 tgggctggcc accttcaccc acgcattgcg caccgccatc gctgacgagc gcggtttcga
      901 cgacgggttg ggcgtgatca gcctggtcga cttcgctgag agtgaagccg ggcccgacgt
      961 cgtctactcg catctcaacg gcgacccggc ttcggtgatc aggacgatca acaccctcaa
     1021 caggttcgac gtggtcctga ttcagcacga gtacgggatc tatgtcggac ccgacggctc
     1081 cgaggtgctc gacatcctcg ctgggcttca cgtcccatcc gtggtgaccc tccacaccgt
     1141 ccttagccgg cccagcgaca gccagcgctc catccttgag aacgtggtgg ctcttgccga
     1201 ccagacgatc gtcatgagcg ataccgccag tcagcgtctg atcgagaact acgacgtcga
     1261 cccagcaaag atcacaaccg tcccccatgg agccaggatc agcctgagcg ggccttcgct
     1321 ggcagacggg catcgaccca ccttcctcac ctggggcttg atcggccccg gaaagggcct
     1381 ggagacggca atcgatgcct tcgcggctct caaagacgtc gatcccctgc cccgttacat
     1441 cattctcggg aagacccacc ccaaggttca ggcggcatcc ggtgacgcct atctcaacgg
     1501 gctccagaca cgagtcgagg aactcggact ccaggatgtg gtcgagttcg acgggcggta
     1561 tctcgacatc gacgccctca ccctggaggt gcgaagcgcc gacttcgtgg ttcttcccta
     1621 tgagtcgacg gaacaagtca cctctg
//
LOCUS       DATITW010000710         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_475107, whole genome shotgun sequence.
ACCESSION   DATITW010000710 DATITW010000000
VERSION     DATITW010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475107"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..549
                     /locus_tag="VLS86_09900"
     CDS             46..549
                     /locus_tag="VLS86_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HSO50846.1"
                     /translation="MLYETKFASIENFNKGGIEVIDDDPKHYAFSNMFEVARDSKPYE
                     KVVVGMNREYTLETIRAEGTSEWRTSPHDEFALVMDGEVLIRLLKLDNPRDFHSPDDA
                     GSRRIEGKPVGEPMGVVKAKRGHMTLLPADAAYQFHADAPGVVLLQTIKGPDSLERWG
                     DICITTV"
     gene            571..1560
                     /locus_tag="VLS86_09905"
     CDS             571..1560
                     /locus_tag="VLS86_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HSO50847.1"
                     /translation="MTTLQEKPTVLASAQNEQGYREFSLGEFHFRRDEYFVYLTWPTG
                     SHILSADAFLRALQRDVAWEFFYGIVNFDGVLGTVNHYGTVDLFAGRYNDSYRKAELD
                     HVENFPTPQIKATFEEMLDDWTNQTFDPFAAPRETGSAFGVKNGDNHKAVTRHRVAAA
                     RMVSCTGDEPLRSDETGHRINEHFKDVSQEEPEIIAEPGYEDEVKSFSLFAYLSRSDV
                     TWNPSVVSVCKDSLFCPTTEEYILPIIHGNDRVEWFVQLSDEIRWDVEDRDAGTVRAK
                     VVMRAGDVAAMPADIRHQGYSRKRSMLLVWENADAALPELIATGQTPTYPAEF"
     gene            1636..>2208
                     /locus_tag="VLS86_09910"
     CDS             1636..>2208
                     /locus_tag="VLS86_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSO50848.1"
                     /translation="MEDRLYVGGRFTTSLAGATIEVRNPHDGAKLADVAEARAEDVDL
                     AVEAASEAFAGWAATSAMERGRLLLRLADAIEADADELARIETLDTGHPIRDTSVLDI
                     PRTSATFRYFGGMADKFQGSIPPVEAGFLNHVYRRPVGVVGQIVPWNFPLMFTSWKLG
                     PALAAGNSVVYKPAELTPLSTLRLAGLIDEV"
BASE COUNT          429 a          692 c          695 g          392 t
ORIGIN      
        1 ttgtgcgcta gttgaacatc gcagccggca caaccgggag gttgaatctt gtacgagacc
       61 aagttcgcat cgatcgagaa cttcaacaag ggtgggatcg aggtcatcga cgacgacccc
      121 aagcactacg ccttctccaa catgttcgag gtcgctcgtg actcgaagcc gtacgagaaa
      181 gtcgtcgtgg ggatgaaccg ggagtacacc ctcgagacga tccgagccga agggacatcc
      241 gagtggcgca cgtcgccgca cgacgagttc gccctggtca tggatggtga agtcctgatc
      301 cggctcttga aactcgacaa ccctcgggac ttccacagtc cggacgacgc aggctcccgt
      361 cggatcgagg gaaagcccgt aggagagccg atgggcgtgg tgaaggcgaa gcgagggcac
      421 atgacactcc tacctgccga cgccgcctac cagttccacg ccgacgctcc tggcgtcgtg
      481 ctgctccaaa cgatcaaagg ccctgacagc ctcgagcggt ggggcgacat ttgcatcacc
      541 acagtttgac cacgaaaagg agtaggtgac atgacaacgc tgcaggagaa gccgaccgtc
      601 ctggcctcag cgcagaacga gcagggctac cgcgagttct cgctagggga gttccatttt
      661 cgccgggacg agtacttcgt ctacctgacc tggcccaccg gctcacacat actctcggcc
      721 gatgccttcc tgcgcgccct ccagcgcgat gtggcctggg agttcttcta cggaatcgtg
      781 aacttcgacg gcgttctggg cacggtgaac cactacggga cggtcgacct gttcgccggc
      841 aggtacaacg actcgtaccg gaaagccgag ctggatcacg tggagaactt cccgactcct
      901 cagatcaagg cgactttcga ggagatgctg gacgactgga ccaaccagac cttcgatccc
      961 tttgccgctc ctcgtgagac gggcagcgct ttcggcgtta agaacggtga caatcacaag
     1021 gccgtcaccc gccatcgggt ggctgcggct cgcatggtta gttgcaccgg cgacgagccg
     1081 ctccggtccg atgagaccgg tcatcgcatc aacgagcatt tcaaggatgt gtcacaggag
     1141 gagcccgaga tcatcgccga gccggggtac gaggacgagg tcaagtcgtt cagcctgttc
     1201 gcatatctct cccgctccga cgtgacctgg aacccatcgg tcgtgtctgt gtgcaaggac
     1261 agtctcttct gcccgaccac cgaggagtac atcctcccga tcatccacgg caacgaccgg
     1321 gtggagtggt tcgtgcaact ctccgacgag atccgctggg atgtcgaaga ccgtgacgct
     1381 gggacggtga gggccaaggt ggtgatgagg gccggcgacg tcgccgccat gcccgccgac
     1441 atcaggcacc agggctactc acgtaagagg tcgatgctcc tggtctggga gaacgctgac
     1501 gcagcgcttc ccgagctgat cgccaccggt cagacgccga catacccggc cgagttctag
     1561 ccgaccggct gggcccgcat gaggctcttg gagcgacagc cgaggtccgg tagggcccac
     1621 gccgggggcg actgagtgga ggaccggctc tacgtcggtg gccggttcac gacttcgctg
     1681 gctggtgcca ccatcgaggt tcgcaaccca cacgacgggg ccaagctggc cgatgtcgcc
     1741 gaggcccggg ctgaggacgt cgacctcgcg gtcgaagctg cctcggaggc cttcgcgggt
     1801 tgggcggcga cctcggcaat ggaacgcggc cgtctgctac tccggctagc cgatgcgatc
     1861 gaggcggacg ccgatgagtt ggcccgaatc gaaaccctcg acaccgggca tccgatcagg
     1921 gacacgtcgg tcctcgacat cccgaggacg tccgccacat tccgctattt cggtgggatg
     1981 gccgacaagt tccaggggtc cattcccccg gtcgaggcgg gatttctcaa ccacgtctat
     2041 cggcgccctg tgggagtggt gggccagatc gtgccctgga acttcccgct catgttcacg
     2101 agttggaagc tcggtccggc tctcgccgcc ggtaactctg tggtttacaa accggccgag
     2161 ttgaccccac tgtccacgct tcgactcgcc gggctgatcg atgaagtc
//
LOCUS       DATITW010000711         1796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_235162, whole genome shotgun sequence.
ACCESSION   DATITW010000711 DATITW010000000
VERSION     DATITW010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235162"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VLS86_09915"
     CDS             complement(<1..1184)
                     /locus_tag="VLS86_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HSO50849.1"
                     /translation="MITADDRSFSYAELHSAMNRVAGRLQDLGIRRDERILLVLDDTP
                     TFPAVFLGAMRIGAVPIPVNFLARPQDFGYFLDDSYAVAAVVDSVFMDKVGPEVAKRA
                     GVKLIVANGDSGEAESADDWLADGDFEVDPVFTHPDDMAFWLYSSGSTGAPKGVVHRH
                     ASVHATCENYAVPTLGINEDDVVFSSTKMFHAYGLGNNLTFPLFAGATSVYSTGPPTP
                     AKLLERVATHSPSLYFSVPALYNAILAHPEFDSTDWSSVTLGISAAEPLPPETWRRFH
                     DRTGIEILDGLGSTEMLHIYCTNRAGAVRPGSSGTAVDGYDIELRDEEGAPVTDGESG
                     ELWVRGPSALSYYWHQSERTRDKLAHGWYASVDRYHRDADGYFAYEGRVDDMMKIGGL
                     WV"
BASE COUNT          313 a          569 c          581 g          333 t
ORIGIN      
        1 ctcacccaga ggcccccgat cttcatcatg tcgtcgaccc ggccctcata cgcgaaataa
       61 ccgtctgcgt cccggtgata tcgatcgact gaggcgtacc atccgtgcgc gagcttgtcc
      121 ctggtgcgct ccgattgatg ccagtagtag gacagggcgc tgggccccct cacccatagc
      181 tcacctgact ccccgtcggt caccggcgcg ccttcctcat cccgaagctc gatgtcatat
      241 ccatccacag ccgtccccga gctccccggc ctcaccgcgc ccgcgcgatt ggtgcagtag
      301 atatggagca tctccgtcga gccgagcccg tccaggatct cgatgccggt acggtcatgg
      361 aaccggcgcc aggtttcggg agggagcggc tcggcggcgg agatcccaag cgtcactgac
      421 gaccaatccg tggagtcgaa ctcgggatgg gccaggatgg cgttgtacag ggcgggcacc
      481 gagaagtaga gcgatggtga atgggtggcc actcgctcca gaagcttggc cggggtcggt
      541 gggccggtgg agtagaccga ggtggcgcct gcgaaaagcg ggaaggtgag gttgttgccc
      601 aacccgtagg catggaacat cttggtgctg gagaagacga cgtcgtcttc gttgatgccc
      661 agggtgggaa cggcatagtt ctcacaggtg gcgtgcacgc tggcatgccg atgaaccaca
      721 cccttcggcg ctccggtcga gcccgagctg tacagccaga aggccatgtc gtcggggtgg
      781 gtgaacaccg ggtcgacctc gaagtcgccg tcggcgagcc agtcatcggc cgactccgcc
      841 tccccggaat ctccgttggc gacgatcagc ttcacaccgg ccctcttggc gacctcgggg
      901 ccgaccttgt ccatgaacac ggaatcgacg acggccgcca ccgcatacga gtcgtctagg
      961 aaatagccaa agtcctgggg tcgtgccagg aaattgaccg ggatgggcac cgcgccgatg
     1021 cgcatcgctc ccaagaacac cgccgggaag gtcggtgtgt cgtcgaggac gaggaggatc
     1081 cgctcgtcgc ggcgaatgcc caagtcttgg agccggcccg caacccggtt catcgcagaa
     1141 tggagttctg cgtagctgaa agaccggtcg tcggccgtga tcagggctgt cttggtcgcg
     1201 cgccccgcta cgaggttgtc gtcgacgaga gtgctgacat tcgacgcggt ccggtcgccc
     1261 cctgtgccta agttcccttg tcttcatcgg acaacggatg cggcgggtcg tgccggggac
     1321 ggtcgccact ccgggcgcgc ccgatcacga tgtcaccatg actggaatct ctcgtctcac
     1381 gtcgtcagtc tcttcacgac ctcggcgtac gtcgacatct cttcggatgt atgtgctcaa
     1441 cctacgcgaa cgagccgtgt cgctcccgac gaccacctcg tgatggttgc agtcgaccga
     1501 ggtggcagct cgaatcgatc caacttcgtt cgccgtcatg gcctcctcac ggtgttctcg
     1561 ctcggtggcc ccagatgagg caaccgtcga tggggcggtg actgtaatga ctggctgacc
     1621 ggccagtcaa ccgagacgac ccccctccct gcgggcgatc tggcatggtc ggcgccggcg
     1681 ggtttgggag gtggcggcag accaggccaa tttgagcgat caccaccggc ggtctgcggt
     1741 cagggcagca gcacgtattc gaactcggtt ggcttgtggt cgatacccct ggtcgg
//
LOCUS       DATITW010000712         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_635945, whole genome shotgun sequence.
ACCESSION   DATITW010000712 DATITW010000000
VERSION     DATITW010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635945"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VLS86_09920"
     CDS             complement(<1..561)
                     /locus_tag="VLS86_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50850.1"
                     /translation="MERLGSLPWWVVPLSLATVGAFAFGQPLLDLLGRNPDYFIARGL
                     TSWDVILFPLAVLVLPLLLSIPVLALRWVGPRTAGLAHAVVIGALFTMLVASAWIALL
                     GSDTSAAGFALVAVGSGALLAVAFARYQMVRTGVGYASWALLAFGAWFLVGAPSSDIA
                     FASFDNRPDLGEAANPVPIVMVVFDEF"
     gene            complement(564..1922)
                     /locus_tag="VLS86_09925"
     CDS             complement(564..1922)
                     /locus_tag="VLS86_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSO50851.1"
                     /translation="MISPDPGSFRDPASRVVLDGERVLRLLDERGLQGWRALAATDFF
                     KTAVSEGRLIAAVEVDPPPGSPGALEHPRVPLISYPYEWTFSMLKAAALLQLDLLEGA
                     LHAGLTIKDSTPYNVQFVDGRPVFIDIGSFEAYRQGEPWIGYRQFTRQYLFPLMLRAW
                     VGVPFQPWLRGDLEGPTPAQMARILGGKKRFNMGALLHVRLQARMEARMSDRAVREDL
                     RTAGFTPELILANVKKLRSLVTSLEWEHGIDGWSDYGSCTHVSRDRDAKSEFLQSAIE
                     RVRPRRVLDLGANDGHFSLIATEGGAQAIAVDSDEAVLDELYRRSIGKSLSVVLTDLG
                     NPSPAQGWAGVERSGIVERARPDLVIAYGLIHHLIYSASIPPREVLQWLRNFGTPVVV
                     EFVSPADEMVAKLVGNKLPAELHSDREEAEFRALLDEFFEVRSERKLQSDTRVLFDLD
                     PR"
BASE COUNT          404 a          678 c          679 g          309 t
ORIGIN      
        1 gaactcgtcg aacaccacca tcacgatcgg caccggattc gccgcttcgc ccagatcagg
       61 gcggttgtcg aagctggcaa aagcgatatc actggaggga gcccccacca ggaaccaggc
      121 gccgaaggcg agcagcgccc aggaggcgta gccgaccccg gtccgcacca tttggtaccg
      181 ggcgaaggcg accgctaaca gggcgccgga cccaacagcc accagggcga atcccgctgc
      241 agacgtgtcg gagccgagca aggcaatcca cgccgacgca accaacatcg tgaagagggc
      301 tccaatcacg acggcgtggg cgagcccagc agtccgcggg ccgacccagc gcagagccag
      361 caccgggatc gacagaagca ggggcagcac gaggaccgcc agtggaaaca ggatcacgtc
      421 ccaagaggtc agcccgcgtg cgatgaagta atcggggttg cggcccaaga gatcgagcaa
      481 tggctgaccg aaggcgaagg cgccgaccgt ggccaaggag agcggaacga cccaccaggg
      541 gagcgaaccg agccgctcca acctcatcgc gggtcgagat cgaagagaac cctcgtgtct
      601 gattgcaatt tccgttcgga cctgacctcg aagaactcgt cgagcagcgc ccggaactcg
      661 gcctcctcgc ggtcgctgtg gagctcggct ggaagcttgt tcccaaccag cttggccacc
      721 atctcgtcag ccggcgacac gaactcgacg acgaccggag tgccaaagtt gcgcagccat
      781 tggaggacct cccggggcgg gatgctcgcc gagtagatga ggtggtggat gagcccgtat
      841 gcgatcacga ggtcgggccg cgcccgctcg acgatccccg acctctcgac tccggcccaa
      901 ccttgagccg gggaagggtt gcccaggtcg gtgagcacca cggagaggct cttcccgatg
      961 ctccggcgat agagttcgtc gagaaccgcc tcatcgctgt cgaccgcgat cgcctgcgcc
     1021 ccgccctcgg tagcgatcag agagaaatgc ccgtcgttgg cgcccaggtc gagcacacgc
     1081 cgaggacgca cccgctcgat cgccgattgc aggaactcac tcttggcatc ccgatcccgg
     1141 gacacatggg tgcaggagcc gtagtccgac cagccgtcga tgccgtgctc ccattcgagg
     1201 gaagtgacca gagaccggag tttcttcacg ttcgccagga tcaactcagg ggtgaaaccc
     1261 gcggtccgca ggtcctccct caccgcccgg tccgacatcc gggcctccat ccgagcctgc
     1321 agccgcacgt ggagcagggc gcccatgttg aatcgcttct tgccgcccag gatcctcgcc
     1381 atctgggcgg gagtcggccc ttcgagatca ccacgcagcc atggttggaa cgggactccg
     1441 acccaagccc gcagcatgag tgggaacagg tactgcctgg tgaactgccg gtagccgatc
     1501 cagggctctc cttgtcggta ggcctcaaag gaaccgatgt cgatgaacac cgggcgtccg
     1561 tcgacgaatt ggacgttgta cggggtgctg tccttgatgg tcaacccggc gtggagggcg
     1621 ccctccaata ggtcgagttg gagcagcgcc gcggccttga gcatggagaa cgtccactcg
     1681 tagggatagc tgatgagcgg caccctgggg tgttcgagtg ctccggggct gccgggcggg
     1741 ggatcgacct cgaccgccgc gatcaggcgc ccctcggaga ccgcggtctt gaagaagtcg
     1801 gttgccgcca gggcgcgcca tccctgaaga ccgcgctcgt cgagaagacg gaggactctc
     1861 tccccatcga ggacgactcg tgaggccggg tcgcgaaacg acccggggtc gggcgatatc
     1921 actgcgcggt caggattcgg tggacagggc tgtgtcttcg gctgctgcgt ccacctcgcc
     1981 ctcgatggcc tctgattcga ggttgcgacg ggcgagcttc atccgcatgg ccttgtaggc
     2041 gaccccgatc ccggccaggc caccgatcag
//
LOCUS       DATITW010000713         1750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_35057, whole genome shotgun sequence.
ACCESSION   DATITW010000713 DATITW010000000
VERSION     DATITW010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35057"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /gene="atpG"
                     /locus_tag="VLS86_09930"
     CDS             complement(<1..708)
                     /gene="atpG"
                     /locus_tag="VLS86_09930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HSO50852.1"
                     /translation="MAGAQLRDTRRRIRGIESTKKITRAMELIAASRIPKAIARVQGS
                     KPYTEKLIEVIENVGAAQGGASHRLLERREVQTVGLLIVTSDRGLAGAYASNVIRLAE
                     QRINQLRKEGKDVLLFTVGKKAQSYFRYRGYRIEQAYLGVTDTPGYGDARAVASRVMN
                     SYAESHIDALETFFTNFRTAMTQVPIRRDLLPIAPPTSEPGEDVGRVSYQFEPSPEEI
                     LGRLLPRYVEAVIFNILL"
     gene            complement(718..>1750)
                     /gene="atpA"
                     /locus_tag="VLS86_09935"
     CDS             complement(718..>1750)
                     /gene="atpA"
                     /locus_tag="VLS86_09935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HSO50853.1"
                     /translation="IGDRQIGKTAILVDTIINQKVNQGTPDAVKCIYVAIGQKASTVA
                     EVVATLEAAGAMEYTVVVHAPASSAPALQMYAPYSGSAIGQHWMYQGEHALVVFDDLS
                     KQAVAYREISLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDELGGGSLTGLPVIETK
                     ANDISAYIPTNVISITDGQCYLQGDLFFQGIRPALDAGISVSRVGGAAQIGAMRKIAG
                     PLRLNLAQFRELAAFAEFGSELDSVSLAQLERGRRVVEVLKQPQFSPVPVEEQVLAIY
                     AVTEGHMDDVALRDVARFEIGLREFFRARHTNLLGTIAETGKLPEAADMDSAIVAFKE
                     TFEGSLIEE"
BASE COUNT          314 a          537 c          579 g          320 t
ORIGIN      
        1 aagcaggatg ttgaagatca cggcctccac gtatctaggc aagagccggc ccaggatctc
       61 ctcgggtgag ggctcgaact gataggagac acgcccgacg tcttcccccg gctccgaagt
      121 gggaggagcg atggggagga ggtcacggcg gatcgggacc tgagtcattg cggtgcgaaa
      181 gttggtgaag aaggtctcca gggcatcgat gtggctttcc gcataagagt tcatgacccg
      241 ggatgcgacg gcgcgggcgt cgccgtaccc cggcgtgtcg gtcaccccca aataagcctg
      301 ctcgatgcga taaccccgat accgaaagta gctctgagct ttcttgccga cggtgaagag
      361 gaggacgtct ttcccctcct tgcgcagctg gttgatccgt tgctcggcga gacggatgac
      421 gttggaggcg taggcccctg cgaggccccg atcggaagtc acaatgagaa gcccgaccgt
      481 ttgcacctcg cggcgttcca gcaggcggtg cgacgcccca ccctgagcgg cgcccacgtt
      541 ctcgatgacc tcgatcagct tctcggtgta gggcttggac ccctgaaccc gggcaatggc
      601 cttcgggatc cggctggctg cgatcagctc catggcgcgt gtgatcttct tcgtcgactc
      661 gatgccgcga atgcggcgtc gggtgtcccg gagctgggcg cctgccattt cagtcgctca
      721 ctcttcgatc agggagccct cgaaggtctc cttgaatgcc acgattgcgc tgtccatgtc
      781 ggccgcctcg ggaagcttgc cggtctcggc aatggttccg agcaggttgg tgtgacgtgc
      841 cctgaagaac tcgcgcagcc caatctcgaa acgggccacg tcacggagcg cgacatcgtc
      901 catgtgcccc tcggtgactg cgtagatcgc gagcacctgc tcctcgaccg gcaccggaga
      961 gaattggggc tgcttgagca cctccaccac ccggcggccg cgctctagct gagcgagtga
     1021 aaccgagtcc aactccgatc cgaactcggc gaaggctgcc aattcccgga actgagcgag
     1081 gttgagacgc aacggccctg caatctttct cattgcgccg atctgggccg cgccgccgac
     1141 gcgagacacc gagatcccgg cgtcgagggc cgggcgaatc ccttggaaga agaggtcgcc
     1201 ctggagatag cactgcccgt cagtgatcga gatcacgttg gttgggatgt aggcggagat
     1261 gtcgttggcc ttggtctcga taacggggag accggtgaga gagccgccgc ccaactcgtc
     1321 ggagagcttg gcgcagcgct ccaggagacg gctgtggaga tagaacacgt cgccgggata
     1381 cgcctcacga cccgggggtc ggcgaaggag cagcgagatc tcgcgatagg ccactgcctg
     1441 cttggacagg tcgtcgaaga cgaccagggc gtgctctccc tggtacatcc agtgctggcc
     1501 gatcgccgag cccgagtagg gggcgtacat ctggagggcg ggggcggatg acgccggggc
     1561 atggaccacg actgtgtact ccatcgctcc cgccgcctcg agggtggcta ccacctcggc
     1621 cacggtcgag gccttctgac cgatcgccac gtagatgcac ttgacagcgt ccggcgtccc
     1681 ctgattcacc ttctggttga tgatcgtgtc gaccaggatc gcggtcttgc cgatctggcg
     1741 gtcaccgatg
//
LOCUS       DATITW010000714         1760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_435577, whole genome shotgun sequence.
ACCESSION   DATITW010000714 DATITW010000000
VERSION     DATITW010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435577"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VLS86_09940"
     CDS             <1..1250
                     /locus_tag="VLS86_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSO50854.1"
                     /translation="NLEFLDGQRGAHVNISGISGVATKTSYATFLLHSLFTSGVLGSR
                     AVNTKALIFNVKGEDLLFLDHANAVLDDEQRLRYQQLGLPAEAFRSMSVFAPPRPGGA
                     AVPDVASRSTGVTAFFWTLEEFCHDDLLPFLFADAEDDRAQYTMVVYSVMVQLRRVVA
                     IGDGGVRLEGQEARTFRDLVDLITQKVQSEDDAPVWAGTAIGRGTVNAFVRRLQGSVR
                     HVERLIRGDQPDPEKNRINLDRQVTVVDLHNLHDRAKRFAVGVTVRKAFDDKEGSGQS
                     AELLFLVLDELNKYAPREGNSPIKEILLDVAERGRSLGVILIGAQQTASEVERRIISN
                     CSIRVVGRLDAAEASREEYGFLPAMQRQRATILKPGTMLLSQPELPVPLVLEFPFPAW
                     ATRASEALALPGRKSEDPFAGLT"
     gene            1263..>1760
                     /gene="sbcD"
                     /locus_tag="VLS86_09945"
     CDS             1263..>1760
                     /gene="sbcD"
                     /locus_tag="VLS86_09945"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HSO50855.1"
                     /translation="MKLLHTADWHVGRTIRGRSRDDEHRQVLAEIAAIARDEQVDLIL
                     VAGDIFDTAAPSPAAEEIVYRALLDLAEVAPVVIIAGNHDNPARLRAVAPLLSLGRVS
                     VGATIARPDDGGVIDVPDLAVRIALVPFIGKRGIVRVEEILRLGQAERIGEYADRVGD
                     ILEALT"
BASE COUNT          309 a          566 c          552 g          333 t
ORIGIN      
        1 tcaacctcga gtttctcgac ggccagcgcg gtgcccatgt gaacatctcc gggatctctg
       61 gtgtcgccac caagacgagt tacgccactt tcctgctcca ctcgctcttc acgtcgggag
      121 tgctcggcag tcgggcggtc aataccaagg cattgatctt caacgtcaag ggcgaagacc
      181 tcttgttcct cgatcacgcc aacgccgttc tcgatgacga gcaacgcctt cgttatcagc
      241 aactcggatt gccagccgaa gcgtttcgat cgatgagcgt gtttgcccca ccccggcccg
      301 gcggcgccgc cgtgcccgat gtcgcttctc gctcgactgg agtcaccgcg tttttctgga
      361 ccctcgaaga gttctgccat gacgacctgc tcccgtttct cttcgccgat gcggaggacg
      421 atcgcgctca atacacgatg gtcgtgtaca gcgtgatggt tcaactccga cgcgtggtcg
      481 ccatcggcga tggtggggtc cgtttggaag gtcaggaagc gaggaccttc cgcgacctgg
      541 tcgatctgat cacccagaag gtgcagagcg aagacgacgc cccggtgtgg gccgggacag
      601 ccatcggtcg tggaaccgtc aatgcgttcg tgcgccgact ccagggttcg gtccggcacg
      661 tcgagcggct catccgtggt gatcaacccg acccggaaaa gaaccggatc aatctcgatc
      721 gccaggtcac ggtcgtcgac cttcacaacc tccacgatcg ggccaaacgg ttcgcggtcg
      781 gcgtgacggt tcgcaaggcc ttcgatgaca aggagggctc gggtcagtcc gccgagctct
      841 tattcctggt gctcgacgag ctcaacaagt acgcgccccg cgaagggaac agcccgatca
      901 aagagattct gctcgacgtg gccgaacgcg ggcgcagcct cggggtcatc ctcatcggag
      961 cccagcagac cgccagcgag gtcgagcgac ggatcatctc caactgttcg atccgggtgg
     1021 tcggccgcct cgacgccgcc gaggcgtccc gcgaggaata cgggttcctt ccggccatgc
     1081 agcgccagcg ggccaccatc ctcaaaccgg gaacaatgct gctgtcccag cccgagttgc
     1141 cggtgccgct ggttctcgag ttcccctttc cggcttgggc gacgcgggcc tcggaggcgc
     1201 tggccctccc ggggcgcaag tcggaggacc catttgccgg cctcacatga agtgaggacg
     1261 agatgaaact tctccatacc gccgattggc atgtaggccg aacgatccgc ggacgatcac
     1321 gcgacgacga gcatcgccag gtgctggccg agatcgccgc gattgcccgc gacgagcagg
     1381 tcgacctcat cctggttgcc ggcgacatct ttgacacggc cgctccctca cccgcagcgg
     1441 aggagatcgt ctatcgagcc ttgctcgatc tagccgaggt tgctccggtg gtgattatcg
     1501 ccggcaacca tgacaatccg gcccgcctgc gggcggtggc accgttgctt tcgctgggcc
     1561 gcgtcagcgt gggggcgacc attgcccgac cggacgacgg gggtgtcatc gatgtgcccg
     1621 atctggcggt tcgaattgca ttggtcccct tcataggcaa acgcggcatc gttcgggtcg
     1681 aggagattct tcgactgggt caggccgagc gaatcggcga gtatgccgac cgggtgggcg
     1741 acattctgga ggcactgacg
//
LOCUS       DATITW010000715         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_595797, whole genome shotgun sequence.
ACCESSION   DATITW010000715 DATITW010000000
VERSION     DATITW010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595797"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VLS86_09950"
     CDS             <1..864
                     /locus_tag="VLS86_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HSO50856.1"
                     /translation="MTGDGVNDAPALKSADIGVAMGVTGTDVSKEAADMILADDNFAT
                     IVSAVREGRAILANIRKFLRFLLSSNIGEVFTMFFGVVLAGLIGLDQVDEAVAVPLLA
                     TQILWINLLTDTGPALAIGVDPAPDDVMRRPPRRLTDRVIDRQMWVGILWVGLVMSVV
                     TLAALDLTLPGGLLEGDGTIVEARTMAFTTLVLAQLFNCFNARSDRVTAFHNLFSNPL
                     LWGAIALSLGLQVAVVHVPFLNEAFETTPLGAGDWLVCTALASLVLWADEAKKLVEGR
                     LRRVRARGAME"
     gene            939..2105
                     /locus_tag="VLS86_09955"
     CDS             939..2105
                     /locus_tag="VLS86_09955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019545952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSO50857.1"
                     /translation="MQFGVFTVGDVTVDPTTGRAPSEAERIKSMVAIAEKTEEVGLDV
                     FATGEHHNPPFVPSSPTTLLGYVAARTERLILSTATTLITTNDPVKIAEDYAMLQHLA
                     DGRVDLMMGRGNTEPVYPWFGRDIRDGIPLAIENYHLLHRLWREDVVDWEGNFRTPLR
                     SFTSTPRPLDRVPPFVWHGSIRSPEIAEQAAYYGDGFFANNIFWPKEHYIRLINLYRA
                     RYEHYGHGRSDQAIVGLGGQVFMRRNSQDAVREFRPYFDHAPVYGYGPSLEEFTEQTP
                     LTVGSPQQVIDKTLRFREFFGDYQRQLFLMDHAGLPLSVVLEQLEMLGGEVVPTLRRE
                     FEALRPAHVPDAPTHASLKAAAANDAADIDSADSDPESDRVGEQVFEGRLVVDK"
BASE COUNT          383 a          702 c          693 g          404 t
ORIGIN      
        1 atgaccggcg acggcgtgaa cgacgctccg gcgctgaagt cggcggacat cggtgtggcg
       61 atgggtgtca ccggcaccga cgtcagcaag gaggctgcgg acatgatcct ggccgacgac
      121 aacttcgcca ccatcgtgtc cgcggtgcgt gaggggcggg cgatcctggc caacatccga
      181 aagttcttgc gttttctgct gtcgtccaac atcggtgagg tgttcaccat gttctttggc
      241 gtggtgctgg cgggtctcat cggcctcgac caggtcgacg aagcggtcgc tgtgccgttg
      301 ctcgcgaccc agatcctatg gatcaacctg ctcaccgaca ccgggccagc cctggcgatc
      361 ggcgtggatc ctgcacccga tgacgtgatg cgtcgtccac cccgccgcct caccgaccgg
      421 gtcatcgacc gtcagatgtg ggtggggatc ctgtgggtgg ggctcgtgat gtcggtggtg
      481 actcttgccg cgctggacct gacgctcccc ggcgggctgc tcgagggtga tggcaccatc
      541 gtcgaggcgc ggacgatggc gttcaccacc ctggttctcg cccagttgtt caactgcttc
      601 aacgcacgat cggaccgcgt caccgccttt cacaacctgt tcagcaatcc gctgttgtgg
      661 ggagcaatag cactctcgct aggcctgcag gtggcggtcg tccatgtgcc gttcctcaac
      721 gaggccttcg agaccacacc actcggcgct ggtgattggc tggtctgcac cgctctggcc
      781 agcctggtgt tgtgggctga cgaggccaag aagctggtcg aagggcgact gcgccgggtc
      841 cgggctcgag gggcgatgga atagcgccct acggcgcccg gttgaccaag ctgcagtagt
      901 tgaaggatca actactgctt gaagtcgaaa ggacccacat gcaattcgga gtgttcacgg
      961 tcggtgatgt caccgtcgat ccgaccacgg gccgggctcc gagtgaggcg gagcggatca
     1021 agtcgatggt ggccatcgcc gagaagaccg aagaggtcgg tctcgacgtg ttcgccaccg
     1081 gggagcacca caacccgcca ttcgtgccct cgtcaccgac caccttgctc ggttacgtcg
     1141 ccgcccgcac cgagcggctg atcctgtcga ccgccaccac cctcatcacc accaatgacc
     1201 cggtgaagat cgccgaggac tacgcgatgc tgcagcacct ggccgacggt cgcgtcgacc
     1261 tgatgatggg caggggcaac accgagccgg tgtacccatg gttcgggagg gacattcggg
     1321 acgggatccc gctggccata gagaactacc acctcctgca tcgtctctgg cgtgaggatg
     1381 tcgtcgactg ggagggcaac ttccggaccc cgcttcgctc cttcacctcg acaccgagac
     1441 cgctcgaccg ggtgcccccg ttcgtttggc atggctcgat ccgaagcccc gagatcgcag
     1501 agcaagccgc gtactacggc gacggcttct tcgccaacaa catcttctgg cccaaggagc
     1561 actacatccg gctgatcaac ctctaccggg cccgttacga gcactacggc cacgggcggt
     1621 cagaccaggc aatcgtgggg ctcgggggtc aggtgttcat gcgtcgcaac tcacaggatg
     1681 cggttcgtga gtttcgcccg tacttcgacc atgccccggt gtacggatac ggtccgtcgc
     1741 tcgaggagtt caccgagcag accccgctga cagtggggag cccacagcaa gtgatcgaca
     1801 agaccctgag gttcagggag ttcttcgggg actaccaacg ccagctcttc ctgatggacc
     1861 atgccggtct ccctctgagc gtcgttctcg agcagctgga gatgctgggc ggtgaggtgg
     1921 taccgaccct ccgccgagag ttcgaggccc tacgtccggc tcatgtcccc gacgccccaa
     1981 cccatgccag cctcaaggcc gcggcggcga acgacgccgc agacatcgac tcggccgatt
     2041 ccgaccccga atcagacagg gtcggagaac aggtcttcga gggccggttg gtcgtcgaca
     2101 agtagggcct gttctgctcg aaggctctcc aggtcgcggt agatcgaagc cacggatgcg
     2161 atcttgccct ccttgcggaa tc
//
LOCUS       DATITW010000716         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_675440, whole genome shotgun sequence.
ACCESSION   DATITW010000716 DATITW010000000
VERSION     DATITW010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675440"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="VLS86_09960"
     CDS             complement(<1..1375)
                     /locus_tag="VLS86_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO50858.1"
                     /translation="MLDRDGVGIYFEVYGEGDTTLLLIPPAPITHSRIWKAMIPHLAR
                     HYRVVTFDGRGNGRSGRPVDKEAHGRAVVQEDILAVLGATSTTRVVVVAHCHANWWAV
                     ELASSHPELVQALVAIEPGVPHLGTSQPHWVETGPHWDEVLEDPSGWELNNRHAMVTR
                     HRQWIEFFFGEQLVEPHSTKQYQDAVGWAMESSGEVLAAGEEGFELDWPERDTVERIC
                     RELDVPTLTIHGELDICQHVDRGRAFAEITNGELIVIEGAGHLTVAREPVKVNRAIED
                     FVDRTEGIPMKQRVWSRGLDRRKRALYVSSTIGLGHARRDIAIARELRKLEPDLEIDW
                     LAQDPVTRVLAGEGETIHPASDWLASESAHFVSEASGHDLHCFQALRRMDEILVANFM
                     IFQEVLEEGLYDIVLGDEAWEVDHFWHENPELKRGAHVWMTDFVGFLPMPSGGGSEEF
                     LTADYNAE"
     gene            complement(1402..2283)
                     /locus_tag="VLS86_09965"
     CDS             complement(1402..2283)
                     /locus_tag="VLS86_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO50859.1"
                     /translation="MRARQPDRQGLVERGGVRIHHETYENSGPTVVLVPTWCLLHSRS
                     WKFQIPYLSRHYRVVTYDPRGNGGSDRPRGPEQHSWRHYLDDLLAVLDVTRTEHAVLV
                     GVSFSGYWANLASVLHPDRVRGVVSIAPVAALGIGHPERARHSYTEVLDTDEGWAKDN
                     VHFIRSNYREYVEFFISEIFSEAHSTKAIEDCVGWAMETDAEMLISSDLADLVPDPDM
                     PAFYRTMTRPLLLIHGDQDRIIPLESSRRLADLTGGALVTLQGAGHCPQVRDPVKVNL
                     LIRECVDRLAAARGVPA"
     gene            complement(2273..>2851)
                     /locus_tag="VLS86_09970"
     CDS             complement(2273..>2851)
                     /locus_tag="VLS86_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020512228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSO50860.1"
                     /translation="RSILAAAKRVLLDGGYARLSTRIIAEQAGVPLSQIHYHFGSKQK
                     LVLAVLDDENRRLLDRQKRMYESELPIWKQWEQACDFLEDDLASGYVRVLHEMIAAGW
                     SEPEIANAVWEQLGGWYRLLAEVAERAEGLAGGLGPFTAGEIAALAGLQFVGAESLIL
                     LGIGEDLLPARSALRKLGSLIRAIEEAPPDAG"
BASE COUNT          507 a          956 c          904 g          484 t
ORIGIN      
        1 tctcggcgtt gtagtcagcg gtgaggaact cctcggagcc cccacccgac ggcatgggga
       61 ggaacccgac gaagtcggtc atccagacat gggcgccccg cttcagctca gggttctcgt
      121 gccaaaagtg gtcgacctcc cacgcctcgt cgccgagcac gatgtcgtag agaccttcct
      181 cgaggacctc ctggaagatc atgaagttgg ccacaaggat ctcgtccatg cggcgcaggg
      241 cctgaaagca atgcaggtcg tggcccgagg cttcggagac gaagtgggcc gattcgctgg
      301 ccagccaatc gctggcaggg tggatggtct cgccctcccc ggccagcacc cgcgtcaccg
      361 ggtcctgggc cagccagtcg atctccaggt ctggctccag cttcctcaac tcccgagcga
      421 tggcaatgtc gcgtcgggca tgacccagac cgatcgtcga cgagacgtag agggcgcgct
      481 ttcgccgatc gaggccccgt gaccagactc gttgtttcat gggaatcccc tccgttcgat
      541 cgacgaagtc ctcgatggcg cgattgacct tgaccggctc ccgggcgacg gtgaggtgcc
      601 ccgccccctc gatgacgatc agctcgccgt tggtgatctc ggcaaaggcc ctcccccgat
      661 ccacgtgctg gcagatgtcg agctctccat ggatggtcag cgtggggaca tcgagctcgc
      721 ggcagattcg ctcgacggtg tcgcgctccg gccaatcaag ctcgaacccc tcctccccgg
      781 cggcaagcac ctcccccgac gactccatcg cccagcccac cgcgtcctgg tactgcttgg
      841 tggaatgggg ctccaccagt tgctcaccga agaagaactc gatccactgc cgatgacggg
      901 tgaccatggc atgacggttg ttgagctccc atccgctggg gtcctcgagc acttcgtccc
      961 agtgcggccc ggtctccacc caatgcggtt gcgatgtccc cagatgcggg accccgggct
     1021 cgatggcgac cagggcctgg accagttccg gatgagagga agcgagctcc accgcccacc
     1081 aattggcatg gcagtgggca acgaccacga cccgggtagt gctcgtcgcc ccgaggacag
     1141 cgaggatgtc ttcctggacg acggcgcggc catgggcctc cttgtcgaca gggcgcccgg
     1201 agcgaccgtt gccccggccg tcgaacgtga cgacccggta atggcgggcc aggtgcggga
     1261 tcatggcctt ccagatcctg gagtgggtga tgggcgccgg tgggatgagc agcagcgtgg
     1321 tgtccccttc cccatacacc tcgaaataga tgcccacccc atcacggtcg agcacgccct
     1381 catggtcggg ataacgcgcc ctcacgccgg gacgccccgc gccgccgcca gccggtcgac
     1441 gcattccctg atcagcagat tgactttcac tgggtcccgc acctggggac agtgtcctgc
     1501 cccctggaga gtgaccaggg ccccgccggt gaggtcggcg agtctccgac tcgactccag
     1561 gggaatgatt cgatcctggt cgccatggat caggagcagt ggccgggtca tcgtccgata
     1621 gaacgccggc atgtcgggat cgggcacgag atcggcgaga tccgaggaga tcagcatctc
     1681 ggcgtcggtt tccatcgccc aaccgacgca gtcctcgatg gccttggtcg agtgggcctc
     1741 gctgaagatc tcggagatga agaactcgac gtactcccga tagttgctcc gaatgaagtg
     1801 gacattgtcc ttggcccaac cctcgtcggt gtcgagcacc tcggtgtagg agtgccgggc
     1861 tcgctccggg tggccgatcc cgagcgcggc cacgggagcg atggaaacga cgccccgaac
     1921 tcgatcggga tggagcaccg aggcgagatt ggcccaatag ccgctgaagg agaccccgac
     1981 gagaacggcg tgctcggttc tggtgacgtc gaggacggcg agcaggtcgt ccagatagtg
     2041 ccgccagctg tgttgctccg ggccccgggg acgatccgac cccccgttgc cccgcgggtc
     2101 gtaggtgaca acccgatagt gacgggacag atacgggatc tggaacttcc aggaccggga
     2161 gtggagcaga caccaggtgg ggaccagaac cacggtcggg cccgagtttt cgtaggtctc
     2221 gtgatggatc ctcaccccgc cgcgctccac caaaccctgc cggtcgggct gtctagcccg
     2281 catccggtgg cgcctcctcg atggcccgga tcagcgaacc gagcttgcgc agtgcggacc
     2341 tggcaggaag caggtcctcc ccgatgccga gcaggatcaa cgattcggca ccgacgaact
     2401 gaagcccggc gagggccgcg atctcgcccg cggtgaatgg gccgagacca cccgccagcc
     2461 cttccgccct ttctgcaact tcggcgagga gccggtacca acctccaagc tgttcccaaa
     2521 ccgcgtttgc gatctcgggc tccgaccagc cggccgcgat catctcgtga agcacccgca
     2581 catagcccga cgccaggtcg tcctcgagaa agtcgcaagc ctgctcccac tgcttccaga
     2641 tcggaagctc cgactcgtac atccgcttct gtcgatcgag cagcctccgg ttctcgtcgt
     2701 cgagcactgc cagcaccaac ttctgcttcg agccgaagtg gtagtggatc tggctcaacg
     2761 ggacacctgc ctgctcggcg atgattcggg tggagagccg ggcataaccc ccgtcgagca
     2821 ggactcgctt ggcggcggcc agaatcgatc t
//
LOCUS       DATITW010000717         1923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_716329, whole genome shotgun sequence.
ACCESSION   DATITW010000717 DATITW010000000
VERSION     DATITW010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716329"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VLS86_09975"
     CDS             complement(<1..522)
                     /locus_tag="VLS86_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSO50861.1"
                     /translation="MNPWTPLVAAILGWGSAAVLTRALLGSGVSTFTVVPMRFGIALI
                     ILLLVSRFWPRFRGADRRHWTRGFILGGVALALPNTFFTMGLEDLPVSLGGLLVALIP
                     AATVGAAHFLVEGERFDPKSIPGLFVPLIGTGVLVGVGGSAMEGVGNMWRGVTRSLIA
                     VGLAGLGGALTRRF"
     gene            594..1703
                     /locus_tag="VLS86_09980"
     CDS             594..1703
                     /locus_tag="VLS86_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50862.1"
                     /translation="MTKRLLSVLALLALVAGACGGTTEGTTTTGPDNETSTTRPETTT
                     TEAAPEAMLLSYALTAGDEYQYEVGLDQHIEMTATGDASLMGDEEMPGDAVIDIAGTA
                     AFNHVVSEGPEPGTFEIHITGEFTDVSVTGTVDGEPVDSNEAPDFAALEPIDVTVVVD
                     EQGNLIQDGVSGDDPLGGMFGDLGALGGSSPAPGLDPGQFIGPPFPDEEVAVGDTWTE
                     EIETPGLTGDDSIVTTVTSTVTGAETLDGAEVLVIESQAATSLIEFDLAEFFAGMFGA
                     FLPEDATEEETAELEQMLEELQFLITIDDTTSDSTTWFDAEAGVARQSEITAVTSIMM
                     DMNVPDEDTGEMVGFVMDMALDQDITYRLISGPDA"
     gene            complement(1713..>1923)
                     /locus_tag="VLS86_09985"
     CDS             complement(1713..>1923)
                     /locus_tag="VLS86_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50863.1"
                     /translation="AEARERAAKAETEALFLRERLAELRATGDTSPGTPRSAAPEEVV
                     DQRRPVPSFSVYLWRSAIAGLRRKR"
BASE COUNT          363 a          652 c          623 g          285 t
ORIGIN      
        1 gaagcgccgg gtcagcgccc cacccagacc ggcgaggccc accgcgatca gagagcgggt
       61 gacgccacgc cacatgttcc ccacaccttc catcgcactt cccccgactc cgaccagaac
      121 cccggtgccg atgaggggga cgaagaggcc gggtatcgat ttggggtcga agcgctcgcc
      181 ctcgaccagg aaatgggctg ctcccaccgt cgccgcgggg atcagggcga ccagcagccc
      241 tcccaggctc accgggagat cctcgagccc catggtgaag aaggtgttgg gcaacgccag
      301 cgccactccg ccgaggatga agcccctggt ccaatggcgt cgatcggcac cacgaaagcg
      361 cggccagaat cgggacacca gcaagaggat gatcagggcg atgccgaatc tcatggggac
      421 cacggtgaac gtggacactc cgctgccgag aagggccctg gtcagcaccg ctgcggatcc
      481 ccacccgagg atggcggcca cgagcggggt ccacggattc atggggcggc acggtacaag
      541 acgctcgagt tctggctcaa aagaaagcaa ccggatgccg ataacctgtg tctatgacca
      601 agcgtttact gtccgtcctt gccctgctgg ctctcgttgc cggcgcatgc ggcggcacca
      661 ccgaaggcac gaccaccacc ggccccgaca acgagacgtc gaccaccagg cccgagacca
      721 ccaccacgga ggctgcgccg gaggccatgc tgctctccta cgccttgaca gcaggtgacg
      781 agtaccagta cgaagtcggt ctcgaccagc acatcgagat gaccgccacc ggtgacgcct
      841 cactgatggg tgacgaggag atgccgggcg atgccgtcat cgacatcgcg gggacagcgg
      901 ccttcaatca tgtcgtttcc gaaggacccg aaccgggcac attcgaaatc cacatcaccg
      961 gtgagttcac cgacgtgagt gtgacgggca ccgtcgacgg ggagcctgtc gactcgaatg
     1021 aggcgccgga ttttgcagcc ctcgagccca tcgacgtcac cgtggtggtc gacgagcagg
     1081 gcaacctgat ccaggatggg gtgtctggcg acgacccgct gggcgggatg tttggtgatc
     1141 tgggtgccct cgggggcagc agcccggccc ccggtttgga ccccgggcag ttcatcgggc
     1201 ccccgttccc ggacgaggag gtcgccgtcg gcgacacctg gaccgaggag atcgagaccc
     1261 ccggcctcac cggagacgac tcgatcgtca ccacggtgac cagcacggtg accggggcgg
     1321 agaccctcga cggcgccgag gtcctggtca tcgagagcca ggccgcgacg tcgttgatcg
     1381 agttcgacct cgccgagttc tttgccggga tgttcggggc cttcctgccc gaggacgcca
     1441 cggaggagga gaccgccgag ttggagcaga tgctcgagga gcttcagttc ctgatcacca
     1501 ttgacgacac cacctcggac agcaccacct ggttcgacgc cgaggccggg gtcgccaggc
     1561 agtccgagat caccgccgtc acctccatca tgatggacat gaatgtcccc gacgaggaca
     1621 ccggcgagat ggtgggcttc gtgatggaca tggccctcga ccaggacatc acctatcggc
     1681 tgatatccgg tcctgacgcc tgacgccgcg actcaccgct tccggcgtag cccggcgatg
     1741 gcgctcctcc acaagtagac cgagaaactg gggaccggtc tcctctggtc gaccacctcc
     1801 tccggcgcag ccgatcgggg agtgcccggt gacgtgtcac cggtggcgcg cagctcggcc
     1861 agccgctccc ggaggaaaag cgcctcggtc tcggccttgg ccgccctctc ccgtgcctcg
     1921 gcg
//
LOCUS       DATITW010000718         1593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_395312, whole genome shotgun sequence.
ACCESSION   DATITW010000718 DATITW010000000
VERSION     DATITW010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395312"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VLS86_09990"
     CDS             complement(<1..746)
                     /locus_tag="VLS86_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO50864.1"
                     /translation="MTRYVLLRTAWAIPVLIIVTFLTFWAVKSATDPVQSYLRTNPRA
                     TPEMVQEYEEVHGLIGSTPEQYVRWLGGFLTLNWGNSIKGNTPVWPDLKSALANSLVL
                     GITASVIGISVGLSIGILSALRQYSKFDTLSTGAAFVGISIPPFVSAVLLQVFFAIAL
                     TSWLGLQSPFLPVAGIYPPGQTEFDLGLRIKHMILPVTVIAIQIIAVYSRYMRASLLD
                     VKESDYMRTARSKGISEKRVVMKHAMRNAL"
     gene            complement(827..>1593)
                     /locus_tag="VLS86_09995"
     CDS             complement(827..>1593)
                     /locus_tag="VLS86_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50865.1"
                     /translation="GDGWTLNGAGMWEKDGVVPEIKWMVNTPNPRREAAQALMIPEFA
                     AAGFNVVADNGDAATVFQQRLPAGDYDLAMYINTASPDPTVTPIMSCDQVPSAENNNQ
                     GQNSVFWCNEEATALMDQSDAEIDETARADLIHQIGQYLVDDHVMLPLFQFPNIAAWR
                     NDRIEGDAPAADAANYRAFNNSSQLWTPIDKEEIVVGAEQWPECINPVTECANSSWMV
                     WTTSFAVLPAIWDTTSDGDFALTNLVSGEPTVTVNE"
BASE COUNT          293 a          469 c          503 g          328 t
ORIGIN      
        1 atgagcgcgt tccgcattgc gtgcttcatc accaccctct tctcggagat ccccttcgac
       61 cgggcggtgc gcatgtagtc ggactccttg acatcgagca gcgatgccct catgtatctg
      121 ctgtagaccg cgatgatctg aatcgcgatc accgtgaccg ggaggatcat gtgcttgatc
      181 cttagcccga ggtcgaattc ggtctggcct ggcgggtaga tcccggccac gggtaagaac
      241 ggggactgaa gcccgagcca gctggtgaga gctatcgcaa agaagacctg gaggagcacc
      301 gccgacacga acggtgggat ggagatgccc acgaaggcgg cgccggtgga cagcgtgtcg
      361 aacttggagt actggcgcaa agcggagagg atcccgatgc tgagcccgac cgagatgcca
      421 atcacgctcg cagtgatccc gagcaccagg ctgttggcga gggcgctctt gaggtcgggc
      481 cataccgggg tgttgccctt gatcgaattg ccccagttga gggtcagaaa accgccgagc
      541 cagcgcacgt attgctcggg agtggagcca atcagcccgt ggacttcctc gtactcctgg
      601 accatctccg gggtggctcg cgggttggtt cggaggtagc tctgcaccgg atcggtggct
      661 gacttgaccg cccagaaggt gagaaaggtc acgatgatga ggaccgggat tgcccaagcc
      721 gttcgcagca gaacgtatct cgtcatctag ccactcccct tggtgtcaca tcgggtcgtg
      781 ttgtctgggg tgcccgtgaa cgggcacccc agacagccta cttcatctac tcgttgaccg
      841 taacggtcgg ttcgccggaa acgaggttgg tgagtgcgaa gtcaccatca ctcgtcgtat
      901 cccaaatggc aggcaggacc gcaaacgatg tggtccacac catccatgag gagttggcgc
      961 actcggtcac cgggttgatg cactccggcc actgctcagc acctacgacg atctcctctt
     1021 tgtcaatcgg agtccatagt tgtgagctgt tgttgaaggc ccggtagttg gccgcatcgg
     1081 ctgcgggcgc gtcgccctcg atccggtcgt tccgccaggc ggcgatgttc gggaactgga
     1141 acaacggcag catgacgtgg tcgtcgacca ggtactgacc gatctgatgg atcaggtcgg
     1201 cacgtgccgt ctcgtcgatc tcggcgtcgg actggtccat gagggcggtt gcttcctcgt
     1261 tgcaccagaa caccgagttc tgaccctggt tgttgttctc cgcgcttggc acctgatcac
     1321 aactcatgat cggagtcacg gttgggtccg gtgaagcggt gttgatgtac atcgccaggt
     1381 cgtagtcacc tgccgggagt cgctgctgga acacagtcgc cgcgtcaccg ttgtcggcaa
     1441 ccacgttgaa tccagccgcg gcgaattccg ggatcatgag agcctgagcg gcctcacgac
     1501 gtggattggg cgtgttgacc atccacttga tctcggggac gacgccgtcc ttctcccaca
     1561 tgcctgcgcc gttcagggtc cagccgtctc cct
//
LOCUS       DATITW010000719         1881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_596003, whole genome shotgun sequence.
ACCESSION   DATITW010000719 DATITW010000000
VERSION     DATITW010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596003"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1621
                     /locus_tag="VLS86_10000"
     CDS             <1..1621
                     /locus_tag="VLS86_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSO50866.1"
                     /translation="IPIRRPDAGSLGGLLSSVSESLLGGATRVDTGDLYRLINRIVEV
                     ALDTEALADLALISIEGSDPAVFRRSWRGQLEKLSASIGDIQDQAVALANVSFRDAAS
                     TFPQFARYLGRRMGKDVRFEIIGDDVQLDRQIVDLLREPLRHLLVNAVDPGIESSAER
                     IAVGKTATGVVRVAARVVDEGVEVSVSDDGGGIDWDKVAEVATENNFPFVHPDLSPLL
                     FQPGFSTAGETTDFSGSGEGLAAARDTVERVHGAVHIESVKGRGTTVTMTLPMSMVLQ
                     NVVVVAAGDQFWGFPEASVEAAMPLSKAEIQTTSGGRELHFETGAIPCVSLSQAVGNG
                     VDDDESEILVMNTRFGLVAVTVGELIDRRRVAVKNLGPILEGPGHVTGAALLGGGQVL
                     VVIDPNFLGILARRRPRLVGRRPRILVVDDSAGVRQLLSATLNGAGFEVEVAGGAREA
                     VMAMAEDDFDAMVVDYSMPRSNGVELVRAMRAAEVRVPIVMVSGVATQEEKRAAWEVG
                     VNAYLDKFDLRQGVLTTTLRRLIGLEDAGTA"
     gene            1669..>1881
                     /locus_tag="VLS86_10005"
     CDS             1669..>1881
                     /locus_tag="VLS86_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSO50867.1"
                     /translation="MSELSASEILVYLIPVVGIACVIWALAITRHGRQREREVVRLSE
                     EAHRIKNDFVAMVSHELRTPLTSIAGF"
BASE COUNT          312 a          569 c          649 g          351 t
ORIGIN      
        1 gattccgatc cggcgccccg acgcaggaag tcttggcggc ttgctgtcct ctgtgtccga
       61 atcgctcctc ggcggagcga cccgggtcga caccggggac ctctatcggc tgatcaaccg
      121 gatcgtcgaa gtcgcattgg acaccgaagc gctggccgac ctggccctga tatcgatcga
      181 agggtcggat cccgccgtgt tccgccgctc gtggcgaggc cagctggaga agctgagcgc
      241 gtcgatcgga gacattcagg accaggcagt ggccctggcc aacgtctcgt tccgcgatgc
      301 cgcctccacc ttccctcaat tcgcccgcta cctgggtcgc cgaatgggca aggacgtccg
      361 gttcgagatc atcggggacg acgttcaact cgaccgtcag atcgtcgacc tgttgcgaga
      421 gccgctgcgc catctcctcg tgaacgcggt cgaccctggg atcgaaagct ccgccgaacg
      481 aatcgccgtt ggcaagacgg cgaccggagt ggtgcgggtg gcagcgcggg tggtggatga
      541 gggggtggag gtctcggtct ccgacgacgg gggtgggatc gactgggaca aggtggccga
      601 ggtcgccaca gagaacaact tccccttcgt gcatccggac ctgagcccgc tcttgttcca
      661 gcccgggttc tccacggccg gcgaaaccac cgatttttcg ggcagcgggg aaggcctggc
      721 ggcggctcgg gacacggtgg aacgggttca tggagccgtt cacatcgagt cggtcaaggg
      781 tcgggggacc acggtgacga tgaccctccc catgtcgatg gtcctccaga atgtcgtcgt
      841 ggtcgcggcc ggtgatcagt tctggggctt ccccgaagcc tcggtcgagg ccgccatgcc
      901 tctttccaag gctgagatcc agaccaccag cggtgggcgg gaacttcact tcgagaccgg
      961 ggccatcccg tgtgtgtcgc tttcacaggc tgtgggcaat ggggtcgacg acgacgagtc
     1021 ggagatcctg gtgatgaaca ccaggtttgg cctggtggcg gtcaccgtcg gcgagttgat
     1081 cgatcggcgc cgtgtcgcgg tcaagaacct ggggccgatc ctggaaggac cgggacatgt
     1141 caccggggcg gcgctgctcg gagggggcca agtcctggtc gtcatcgacc cgaatttcct
     1201 cgggatcctg gcgaggcggc gtccgcgtct ggtgggacgg cgcccacgca tcctggtggt
     1261 cgacgactcg gccggggtgc gccagcttct ctcggcgacg ttgaacggcg ccggcttcga
     1321 ggtcgaggtg gccggcggcg cccgtgaagc ggtgatggcg atggcggagg atgacttcga
     1381 cgccatggtt gtcgactatt cgatgccccg ttccaacgga gtcgagctgg tgcgggcaat
     1441 gcgggccgcc gaggtgcggg ttcctatcgt tatggtctcc ggtgtcgcca cccaggagga
     1501 aaagcgggcc gcgtgggaag tcggtgtcaa cgcctatctg gacaagttcg acctccgcca
     1561 gggggtcctc accaccactc tgcggcgtct gatcggtctc gaggacgccg ggacggcctg
     1621 acccgtaaac cttcaggtcg ccgcgaaact ccccgataga agctgagaat gtctgagctt
     1681 tccgccagcg agatcttggt gtatctcata ccggtcgtgg gcattgcctg tgtcatctgg
     1741 gcattggcga tcaccagaca tggtcgacag cgagagcgcg aagtcgttcg tctgtccgaa
     1801 gaggcacatc ggatcaagaa cgactttgta gccatggtca gccacgagct gcggactccg
     1861 ctcacctcga tcgcaggttt c
//
LOCUS       DATITW010000720         1640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_475550, whole genome shotgun sequence.
ACCESSION   DATITW010000720 DATITW010000000
VERSION     DATITW010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475550"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VLS86_10010"
     CDS             <1..461
                     /locus_tag="VLS86_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HSO50868.1"
                     /translation="FGTFMVGEVAMPVGQTISGVEIPTDLVVSQEIPMVLNDAGVIGL
                     SLNGKSFPATQPYVGKVGDWVVTHYFNEGLQIHPMHQHQFPQLVFAKDGIPLDHPYWA
                     DVVNVAPGERYSVLYQLSDPGVWVWHCHILTHVEREEGMFGMVTAWIVEE"
     gene            472..1200
                     /locus_tag="VLS86_10015"
     CDS             472..1200
                     /locus_tag="VLS86_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HSO50869.1"
                     /translation="MLRRINATYEPIDDLLGLYLGEERRAGDRPWLMLNFVSSIDGGT
                     AFEGKSSRLGDDDDRELFLTLRAVPDVILVGAGTVAAEDYGPVVLDEKRRGRRLEAGL
                     SEVPVLAIVSGRLSFDPEARVFSDPEHRPLVITGPDAEPAKLALLGDAADVVILEEVT
                     PAAILARLGAASVILCEGGPSLAGQFASEGLVDELHLTLAPKMISGRSARIAYGPPVE
                     APLEMALDRALIGDRSLFLRYLRA"
     gene            1219..>1640
                     /locus_tag="VLS86_10020"
     CDS             1219..>1640
                     /locus_tag="VLS86_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018383285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSO50870.1"
                     /translation="MGKHAELFERHKAVLPGWAPLYYQEPIAIVDGDGRHVIDAEGNR
                     YLDFFGGILVTMSGYKVPEVVEAIKAQADKMLHTSTLYLIESQIELAEKVAALSGIRD
                     AKVFFTNSGTEANDAALMLATTYRKSNHVLAIRGSYHG"
BASE COUNT          294 a          495 c          552 g          299 t
ORIGIN      
        1 tgttcgggac gttcatggtc ggcgaggtcg cgatgccggt gggtcagacc atctctggcg
       61 tggagatacc gaccgacctc gtggtttcgc aggagatccc gatggtgctc aacgacgccg
      121 gggtcatcgg tctcagtctc aacggcaagt cattcccggc gacccagcct tacgtcggca
      181 aggtggggga ttgggtggtg acccactact tcaacgaggg gttgcagatc catccgatgc
      241 atcagcatca gttcccgcag ttggtcttcg ccaaggatgg gatcccactc gatcatccct
      301 attgggccga cgtcgtgaac gtggcgcccg gggagagata ctcggtcctg taccagctga
      361 gcgacccggg tgtctgggtc tggcactgtc acatcctgac ccatgtcgag cgcgaagaag
      421 gcatgttcgg aatggtcacc gcctggatag tcgaggagta gcgtcgattg gatgctccgg
      481 agaatcaatg cgacctatga gcccatcgat gacctgctcg gcctctatct aggtgaagag
      541 cgccgggccg gagaccggcc atggctgatg ctgaacttcg tgtcctcgat cgacggcggc
      601 acagccttcg aaggcaagtc gtccagactc ggagatgacg acgaccggga actgttcctg
      661 acgctgcgag cggtcccgga cgtgatcctg gtgggagcgg gcacggtggc ggccgaggac
      721 tatggaccgg tcgtcctcga cgagaagcgt cggggacgca ggctcgaagc cggcttgagc
      781 gaggtgccgg tcctggccat cgtctccggt cgtctcagct tcgatcccga agcgagggtg
      841 ttctccgacc cggagcatcg gccgctggtc atcaccgggc cggacgccga accggcgaag
      901 ctggcactgc tgggcgacgc cgccgacgtc gtcatcctcg aagaagtgac gccggcggcg
      961 atcctggccc gtctcggcgc agcctcggtg atcctgtgtg agggggggcc cagcctggcc
     1021 ggccagttcg ccagcgaagg cctggtggac gagctgcacc tgaccctggc gccgaagatg
     1081 atcagcggcc ggtcggcgcg gattgcgtac ggtccgccgg tcgaagcgcc cctggagatg
     1141 gcactcgacc gggccctcat cggcgatcgt tcgttgtttc tgcgatattt gagggcgtga
     1201 atcgccaagg agggaccaat gggtaagcat gccgagctct tcgaacggca caaggcggtc
     1261 ctgccgggtt gggcccctct ctactaccag gagccgatag ccatcgtcga cggggacgga
     1321 cgccatgtca tcgatgccga gggcaaccgc tatctcgatt tcttcggcgg gatcctggtg
     1381 acgatgtcgg gctacaaggt ccctgaggtc gtcgaggcga tcaaggccca ggcggacaag
     1441 atgctccata cctcgacgct gtacctgatc gaatcgcaga tcgagcttgc cgagaaggtg
     1501 gcagcgctgt ccgggattcg cgacgccaag gtcttcttca ccaactcggg caccgaggcc
     1561 aacgatgccg ccctgatgct ggccacgacc taccggaagt cgaaccacgt cctcgcgatt
     1621 cgggggagct atcacggcaa
//
LOCUS       DATITW010000721         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_515501, whole genome shotgun sequence.
ACCESSION   DATITW010000721 DATITW010000000
VERSION     DATITW010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515501"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /gene="gnd"
                     /locus_tag="VLS86_10025"
     CDS             <1..1040
                     /gene="gnd"
                     /locus_tag="VLS86_10025"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HSO50871.1"
                     /translation="LYLGMGISGGEEGARHGPSLMPGGSVAAWERVEPMLKGIAARAP
                     DGAPCCAWIGPGGAGHFVKMVHNGIEYGDMQVIAEAYDLMRRGMGMSPAQISEVFGTW
                     NEGRLRSYLVEITRDILAADEDGTPMVDLILDAAGQKGTGKWTVISSMEFGEPTTLVA
                     EAVYARIVSSDPDRRRRAAQVFPTPTGDMSGVLTEDIEQSLYASKIISYAQGFRLMGG
                     ASAEHDWNLSPGSIASIWRAGCIIRAAFLEDITAAYLREPDLADMTEDGFFADALLAA
                     EPPWRRVVAGAVTAGIPAPAYSSALAYFDSMRSARLPADLIHAQRDYFGDHTFERGDR
                     PRGEFFHHQWP"
     gene            complement(1156..>2069)
                     /gene="serB"
                     /locus_tag="VLS86_10030"
     CDS             complement(1156..>2069)
                     /gene="serB"
                     /locus_tag="VLS86_10030"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017626347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HSO50872.1"
                     /translation="GAVASARAAGDGNIERIFRLSRYPVVSYELLVSGGDLSVIREGL
                     VLAALQLPMDVAIQPDGLERRAKRMVIMDVDSTLIQHEVINLLAREAGVEEQVAEMTH
                     LALTGEMDFERSLRERVALLKGLDQVALTRVFENITLTSGARTFIRSLKRLGMVTAIV
                     SAGFTRFTTVLAADLGIDYSLSNTLVDVDGVLTGELTGEFVDGPRKAQFLKAIADAEG
                     IPLAQVVAVGDGANDLDMLAAAGLGIAFNAKPVVRERADTNISVPYLDAILFIMGIRR
                     QHVEDADASDPEFDAPDHIEVPGIPPV"
BASE COUNT          372 a          673 c          665 g          359 t
ORIGIN      
        1 ttctctacct ggggatgggg atctccggcg gtgaagaagg agcccgacat ggcccatccc
       61 tgatgcctgg cggcagcgtg gccgcctggg agcgggtcga gccgatgctc aagggcatcg
      121 ccgccagggc gccggatggc gctccgtgtt gtgcgtggat cgggcccggc ggcgccgggc
      181 atttcgtgaa gatggtccac aacgggatcg agtacgggga catgcaggtg atcgccgagg
      241 cctacgacct gatgcgtcgg ggtatgggca tgagcccggc ccagatctcg gaggtgttcg
      301 ggacctggaa cgagggtcgt ctccgctcgt atctcgtcga gatcacccgc gacatcctgg
      361 ccgccgacga ggacggcacc ccgatggtcg atctgatcct cgacgccgcc ggacagaaag
      421 ggaccgggaa gtggacggtg atctcgtcga tggagttcgg tgagccgacc accctggtcg
      481 ccgaagcggt ctatgcgcgc atcgtcagct ccgaccccga ccggcgccgg cgtgccgcac
      541 aggtctttcc gacaccgacc ggcgacatgt caggggtgct gaccgaggac atcgaacagt
      601 ccctctatgc atcgaagatc atctcctatg cccaaggatt ccgtctgatg ggcggtgcct
      661 ccgccgaaca cgattggaac ctgagcccgg gttcgatcgc atcgatctgg cgggcaggct
      721 gcatcattcg ggccgccttc ctcgaagaca tcaccgccgc ctaccttcga gaacccgacc
      781 tcgcagacat gaccgaggac gggttcttcg ccgatgccct cctcgcagca gagcccccat
      841 ggcgcagggt ggtggccggg gcagtcaccg ccgggatccc cgcacccgcc tactccagcg
      901 ccctggccta cttcgactcg atgcgctcgg cccgtctccc agccgacctg atccacgcgc
      961 aacgggacta tttcggggac cataccttcg aacgggggga tcgtccccga ggtgagttct
     1021 tccatcatca atggccgtag gccattgatg atcctaagcc tcaggcctta agccataggc
     1081 tgggttcggc gttggggctt gtggctctgg cctgaggctt gaggcctaag gcctgggacg
     1141 atgagcgaag ctcgcctaga ccggtgggat gccggggacc tcgatgtggt caggggcgtc
     1201 gaactcgggg tctgaggcgt cggcatcctc gacgtgttgg cgtcggatcc ccatgatgaa
     1261 gaggatggcg tcgaggtagg ggaccgagat gttggtgtca gcacgctccc ggaccaccgg
     1321 tttggcgttg aaggcgatcc ccaaccccgc cgcggccagc atgtccaggt cgttcgcccc
     1381 atcgccgacc gccaccacct gagccagggg gatcccttcg gcgtcagcaa ttgccttcag
     1441 gaactgggct ttacggggcc cgtcgacgaa ctcaccggtc aactcaccgg tgagaactcc
     1501 gtccacgtcc accagtgtgt tcgacaggga gtagtcgatc ccgagatccg cagccaggac
     1561 agtggtgaaa cgggtgaatc cggcagagac gatcgcggtc accatcccca gccgtttcag
     1621 ggagcgaatg aaggttcgcg ccccgctggt caaggtgatg ttctcgaaca cccgggtgag
     1681 cgccacctga tccaagcctt tcaacagcgc cacccgctcc cgcaacgatc tctcgaaatc
     1741 catctcgccg gtgagggcga ggtgggtcat ctcggcgacc tgttcctcca cccccgcctc
     1801 gcgggccaag aggttgatca cctcgtgttg gatcagagtc gagtcgacat ccatgatcac
     1861 catgcgcttg gcgcgtcgct ccaggccgtc gggctggatc gccacgtcca tcgggagctg
     1921 gagggcggcg aggacgagcc cttcgcgaat taccgaaaga tcgcctccgg agaccaacaa
     1981 ctcgtaagag accacgggat agcgagacag ccgaaagatc cgctcgatgt tcccatcacc
     2041 agccgctctc gccgaggcca ccgccccga
//
LOCUS       DATITW010000722         1886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_275446, whole genome shotgun sequence.
ACCESSION   DATITW010000722 DATITW010000000
VERSION     DATITW010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275446"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VLS86_10035"
     CDS             <1..455
                     /locus_tag="VLS86_10035"
                     /inference="COORDINATES: protein motif:HMM:NF037386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50873.1"
                     /translation="EHVEELADVRVEVGLDWAGFFPYSAEPGTPAAAMEDQVPREVAM
                     ERLRYLSSSQEEVTESRHSEWMGRTDTVLIDQVEDDVPIARSHRQAPEIDGVIRVDRG
                     EVGDWVTVEYTGVFGPDMEAVRVGSPGELADPDRWSGAGRGRAVGAAP"
     gene            452..1687
                     /locus_tag="VLS86_10040"
     CDS             452..1687
                     /locus_tag="VLS86_10040"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HSO50874.1"
                     /translation="MIVEVLAVGTELLLGQIVNSNTSTMGAALAERGFDAHYQQTVGD
                     NLERISSAIRTALGRADALIITGGIGPTQDDITREAVCHVTGREMVFSEEYAEHLREW
                     WARRGREMPESNLRQAQHPKGAELLPNPRGTAPGLALQHEGKWIFCVPGVPAEMENLL
                     ITEVLPRLGAAAGDEQVIASRLIRTWGRPESEVAEILDDLYTGSVNPSLAFLASNSEI
                     KVRITAKAATAPEARRLIEPMEQEVRSRLGDSVFGSDDETIERVLLRLLSDLEYTIGT
                     AESMTGGMVTARLTDLPGSSAVVKGGLVAYDAELKRKLLGVSDISHVVSEETALTVAK
                     GAQALLGVDVTVSVTGSAGPQALEKPVGTVVIGVATPDEARAREMRFSGDRERIRTYA
                     TAAALQLTRLALIGKWWGS"
     gene            1672..>1886
                     /locus_tag="VLS86_10045"
     CDS             1672..>1886
                     /locus_tag="VLS86_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HSO50875.1"
                     /translation="MVGLLGRVFVAAPLPDEVRMALADRLLQVELPGKVVPPESWHIT
                     LRFLGNLDEVVYERFLGLMDTSDLGQS"
BASE COUNT          310 a          585 c          681 g          310 t
ORIGIN      
        1 aagagcatgt cgaggagctg gccgatgtcc gggtcgaggt cgggctcgat tgggccgggt
       61 tcttccccta ctcggccgag ccggggacac cggccgcggc catggaggac caggtgccgc
      121 gagaggtggc catggagcgt ctccgttatc tgtcttcgag tcaggaagag gtgaccgagt
      181 cacgtcactc ggagtggatg ggccgcaccg acacggtgct gatcgaccag gtggaggacg
      241 atgtccccat cgcccgttct caccgtcagg cccccgagat cgacggggtg attcgggtcg
      301 accgaggcga ggtcggcgac tgggtcaccg tcgagtacac aggtgtcttc ggccccgaca
      361 tggaggctgt ccgcgtcggg tcccccggcg agctcgccga tcccgaccga tggtcgggtg
      421 ccgggcgggg acgagcggtg ggtgccgctc cgtgatcgtc gaagtgctcg cggtcggcac
      481 cgagctcctg ctcgggcaga tcgtcaactc gaacacctcg accatggggg cggcgctggc
      541 cgagcgtggt ttcgacgccc actatcagca gactgtcggc gacaacctgg agcggatctc
      601 ctcggcgatc aggactgcgc tgggccgtgc cgacgcactg atcatcaccg gcggcatcgg
      661 gccgacccag gatgacatca cccgggaggc ggtctgtcac gtcaccgggc gggagatggt
      721 gttcagcgag gagtacgccg agcatctccg cgagtggtgg gcgaggcggg gacgtgagat
      781 gcccgagagc aacctccgcc aggcgcaaca tcccaaaggt gccgagttgc tccccaatcc
      841 acgggggacc gcgcccggtc tggcgctgca gcacgagggc aagtggatct tctgcgtgcc
      901 gggggtgccg gccgagatgg agaacctcct gatcaccgag gtgctgccga gactgggggc
      961 ggcggcagga gacgagcagg tgatcgccag tcgtctgatc cggacctggg gacggcccga
     1021 gtcggaagtg gctgagatcc tcgacgacct gtacaccggg tcggtaaacc cgtcgctcgc
     1081 ttttctcgcc tcgaactcgg agatcaaggt ccggatcacc gccaaggcgg ccaccgcgcc
     1141 ggaagcgagg cggttgatcg agcccatgga gcaggaggtc cgctcccgtc tcggcgacag
     1201 tgttttcggc tccgatgacg agaccatcga gcgtgtccta ttgcggttgc tctcggatct
     1261 cgagtacacg atcggcaccg ccgagtcgat gaccgggggg atggtgaccg cccgcctgac
     1321 cgatctgccc ggctcgtcgg ccgtggtcaa agggggattg gtcgcctatg acgcagagct
     1381 gaagcgaaag ctgctcggtg tgagcgatat cagtcacgtc gtcagcgaag agaccgcgtt
     1441 gacggttgcg aagggagcac aggccttgct gggggtcgac gtgacagtct cggtgaccgg
     1501 gtcggccggc ccccaagctc tggagaagcc ggtgggaacg gtggtcatcg gggtggccac
     1561 ccccgatgag gcgcgggccc gggagatgag attctccggt gaccgggagc ggatccgcac
     1621 ctacgccacc gcggccgccc ttcaactcac ccgtctcgct ctcatcggga agtggtgggg
     1681 ctcctaggcc gggtcttcgt cgccgcaccc ctgcccgacg aggtgcggat ggccctggcc
     1741 gaccgcttgc tgcaggtcga gctcccgggc aaggtcgtcc cgccggagag ctggcacata
     1801 acgctgcgat tcctcggcaa cctcgacgag gtggtgtacg agaggtttct cgggctcatg
     1861 gacacgtcgg acctcggtca atcgtt
//
LOCUS       DATITW010000723         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_35718, whole genome shotgun sequence.
ACCESSION   DATITW010000723 DATITW010000000
VERSION     DATITW010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35718"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VLS86_10050"
     CDS             <1..852
                     /locus_tag="VLS86_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSO50876.1"
                     /translation="EGKSGPFLAGILDSVVLSKVRDGLGMDAVDLAITAAAPINSELI
                     TFFHTIGIPLFELYGMSETTGPATTNLPGANKTGSVGRALPGVEVELAEDGEVLMRGG
                     VITSGYYKLPEATEESYDSDGWLHSGDLGRIDEDGYLWIVGRKKEIIITAAGKNIAPA
                     KLETVLGNHPIISKACIIGDNRKFLSVVIALDHEEAPAWAEARGLEYQDLDAFSQLPE
                     VLAEVEKAVREANEKVSSVEQIKKWIVVPDEWTPDSGEVTPSLKLKRRVVVEKYADEI
                     DSLYTGV"
     gene            855..1757
                     /locus_tag="VLS86_10055"
     CDS             855..1757
                     /locus_tag="VLS86_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSO50877.1"
                     /translation="MPITFDGRVAVVTGAGGGLGREYALELGRRGARVVVNDLGGAVD
                     GTGSSTAAADVVVEEIRGSGGEAVPNYDSVSTREGGAAVIQCALDTFGTVDVVVNNAG
                     ILRDHSFANMTFDEIDAVIDVHLRGAFHVSQPAFVVMKEKGYGRFVHTTSASGLFGNF
                     GQANYGAAKAGIMGLSNVLAIEGAKYNVKSNAVAPIARTRMTEDLLGPFADMVDPKQV
                     MPMVVYLCSEANEHTHEIFTVGGGRYARIFLGTNRGWLAGQGVVPEVEQVAEHIDEVR
                     DISQFEVPLSNNEEIMLLAQMLAG"
     gene            1760..>2107
                     /locus_tag="VLS86_10060"
     CDS             1760..>2107
                     /locus_tag="VLS86_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-alpha,7-alpha,
                     12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase"
                     /protein_id="HSO50878.1"
                     /translation="MPVDIERALGASLPGSTYSWTEDDIILYHLGIGAGVPPTDPNEL
                     RYVYEGDLHVLPTYGTIPPFGVMMSLGAVDGLEFDLASVLHGEQELEVHRAIPTAGSV
                     TQTGTVTAIYDKGS"
BASE COUNT          394 a          647 c          703 g          363 t
ORIGIN      
        1 gagggcaagt caggcccgtt cctggccggt atcctcgatt cggtggttct ctccaaggtc
       61 cgcgatggct tggggatgga tgccgtggat ctggcaatca ccgcggccgc cccgatcaac
      121 tccgagctga tcaccttctt ccacaccatc gggattccgc tgttcgagct ctacgggatg
      181 tcggagacca ccggaccagc gaccaccaac ctccccggag ccaacaagac cgggtccgtg
      241 gggagagcgc tccccggggt cgaggtcgag ctcgccgagg acggcgaggt cctgatgcga
      301 ggcggggtga tcaccagtgg ctactacaag ctcccggaag ccaccgaaga atcgtatgac
      361 tccgatggat ggctccactc aggggatctg ggccgcatcg atgaggatgg ctatctctgg
      421 atcgtcgggc gcaagaagga gatcatcatc accgcggcgg gcaagaacat tgctcccgcc
      481 aagctggaaa cggtcctggg caaccacccg atcatctcca aggcatgcat catcggcgac
      541 aaccgcaagt tcctgagcgt ggtgatcgcc ctggatcacg aggaggcccc ggcctgggcc
      601 gaggcccgtg ggctcgagta ccaggatctc gatgccttct cccaactccc cgaagtgctg
      661 gccgaggtgg agaaggcggt gcgggaggcc aacgagaagg tctccagcgt ggagcagatc
      721 aagaagtgga tcgtggtccc tgacgagtgg actccggact ccggagaggt gactccctcg
      781 ctcaagctga agcggcgggt ggtcgtcgag aagtacgcgg acgagatcga ctccctgtac
      841 accggcgtct gagcatgccc atcacattcg atggccgggt ggcggtcgtc accggcgccg
      901 gcggcggcct gggcagggaa tacgcgctcg agctcggtcg gcggggtgcc cgggtggtcg
      961 tgaacgacct gggtggcgcg gtagacggca ccgggtcatc gacggcggcc gcagacgttg
     1021 tggtcgagga gatccggggc agcgggggag aggccgttcc caattacgac tcggtttcaa
     1081 cacgtgaggg gggcgccgcg gtcatccagt gcgccctgga tacgttcggg accgtggacg
     1141 tggtggtcaa caacgccggc atcctgcgcg accactcgtt cgcaaacatg accttcgacg
     1201 agatcgacgc cgtgatcgat gtccatctcc gaggggcgtt tcacgtgtcg cagccagcct
     1261 tcgtggtgat gaaggaaaaa gggtatgggc gctttgtcca caccacctcg gcttcgggtc
     1321 tcttcgggaa cttcggccag gcgaattacg gggccgccaa ggcggggatc atgggtctgt
     1381 ccaacgtcct tgcgatcgaa ggcgcgaagt acaacgtgaa gtccaatgcc gtggccccga
     1441 tcgcccggac ccggatgacc gaggatctgc tcgggccatt cgccgacatg gtcgatccca
     1501 aacaggtgat gccgatggtc gtgtatctgt gctccgaggc caacgagcac acccatgaga
     1561 tcttcacggt tggaggcggg cggtacgcgc ggatcttcct cgggaccaac cgaggctggc
     1621 tggccggcca gggggtggtg cccgaggtcg agcaggtggc cgagcacatc gacgaagtcc
     1681 gtgacatctc acagttcgag gtccctctca gcaacaacga ggagatcatg ctcctcgccc
     1741 agatgctggc tggctgaaga tgccggtcga catcgagagg gcgcttggag cctctttgcc
     1801 cggctcgacc tacagctgga ctgaggacga catcatcctc taccacctcg gcatcggagc
     1861 cggtgtcccg ccgacggatc ccaacgagct tcgctatgtc tacgaagggg atctgcacgt
     1921 gctccccacc tacggcacca ttcccccgtt tggggtgatg atgagcctgg gagccgtcga
     1981 cggtctcgag ttcgacctcg cctcggttct ccacggcgag caggagctgg aggtccatcg
     2041 cgcgatcccc accgcgggct ccgtcaccca gacgggaacc gtgactgcga tctacgacaa
     2101 aggtagc
//
LOCUS       DATITW010000724         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_155320, whole genome shotgun sequence.
ACCESSION   DATITW010000724 DATITW010000000
VERSION     DATITW010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155320"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /gene="rpsK"
                     /locus_tag="VLS86_10065"
     CDS             <1..128
                     /gene="rpsK"
                     /locus_tag="VLS86_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HSO50879.1"
                     /translation="TGSGRDTAVRTLQNMGMEVTWIRDVTPIPHNGCRPKKRRRG"
     gene            128..733
                     /gene="rpsD"
                     /locus_tag="VLS86_10070"
     CDS             128..733
                     /gene="rpsD"
                     /locus_tag="VLS86_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017976781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HSO50880.1"
                     /translation="MARYTGPRHKPCRRARTPLCQSKRCPVDRRPYPPGQHGRGRIRE
                     SDYLIQLREKQKLRTMYGVLERQFRRYYEEAARRPGITVEILLQLLEQRLDNVVWRSG
                     LAATRPQARQLVNHGHFQVNGKKVDIPSYQVRPGDVVTIMDKTSDAIVIKHAVDTQGR
                     EAPAWLHVDLGERKVEVTDRPRRDQIDTAINEQLIVELYSK"
     gene            746..1690
                     /locus_tag="VLS86_10075"
     CDS             746..1690
                     /locus_tag="VLS86_10075"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016664054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HSO50881.1"
                     /translation="MIVQRPQIEEETISDVRSKIVVEPLEPGFGYTLGNTLRRTLLSR
                     IPGAAVTSIQIEGVEHEFSTVPGVVEDVVDIILNIKRIVVRMEDNDVQTLYLSAKGAG
                     EVTAGDLKLPAGVEIVNPDLHIATLSASGRLEMEMTVAPGVGYRSADGNKGASSVIGV
                     IPVDSIFSPVRKVSYKVENTQVGQMTNFDRLVLDIETNGAVEPGEAVSSAGKTLRELM
                     GLFADIGEGVGLELGEVQPEFSGSPDLDLPIEALDLSERPRNCLRRAQIKTVGELVER
                     TSDDLLNITNFGQKSLEEVTAKLDELGLSLADAGGGGS"
     gene            1690..2040
                     /gene="rplQ"
                     /locus_tag="VLS86_10080"
     CDS             1690..2040
                     /gene="rplQ"
                     /locus_tag="VLS86_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HSO50882.1"
                     /translation="MPRPKKGPRFGGSPSHNKKIMANLATELFLHEKVTTTLPRAQAV
                     RPLAEKLITKARKGDLHAKRIVLRTIPNRDAVVKLFDDVAPRYADRQGGYTRITKLGP
                     RRGDGAEEAIIELV"
BASE COUNT          399 a          691 c          661 g          338 t
ORIGIN      
        1 gcaccggctc cgggcgcgac accgcggtca ggacgttgca gaacatgggc atggaggtca
       61 cctggatccg tgacgtgact ccgatccccc acaacggctg tcgacccaag aagcgtcgga
      121 ggggctgatg gctcgttaca caggcccccg ccacaagccc tgtcgtcggg cgcgcacccc
      181 gttgtgccag tccaagcggt gcccggtcga tcgtcgcccc tacccgccgg ggcagcacgg
      241 ccgaggtcgg atccgggaga gcgactacct catccagctc cgcgagaagc agaagctccg
      301 caccatgtat ggcgtcctcg agcggcagtt ccgtcgctac tacgaggagg ccgcgagacg
      361 ccccgggatc accgtcgaga tcctgctcca gctactcgag cagagactcg acaacgtcgt
      421 ctggcgctca ggtctcgccg ccacccgtcc ccaggccagg cagctcgtga accacgggca
      481 cttccaggtc aacgggaaga aggtggacat cccgtcctat caggtccgtc ccggtgacgt
      541 ggtcaccatc atggacaaga ccagtgacgc catcgtcata aagcatgccg tggacacaca
      601 gggacgcgag gctccggcct ggctccatgt cgacctcggc gagcggaagg tggaggtgac
      661 cgatcgccct cgccgggatc agatcgacac cgccatcaat gagcagttga ttgtcgagct
      721 ctactcgaag tgaggtattg acttgttgat cgtccaacgc ccccagatcg aagaggagac
      781 gatttccgac gtccggtcga agatcgtcgt cgagcctctg gagcctggtt tcgggtacac
      841 cctcggtaac actctccgtc ggacgctcct ctcccgcatc cccggggcag cagtcacctc
      901 gattcagatc gaaggggtgg agcacgagtt ctccaccgtg cccggggtcg tggaagacgt
      961 ggtcgacatc atcctcaaca tcaagcgcat cgtggtgcgg atggaggaca acgacgtaca
     1021 gaccctctat ctctcggcca agggcgccgg agaggtgacg gccggcgact tgaagctccc
     1081 ggccggcgtc gagatcgtga accccgacct ccacatcgcc accttgagcg ccagcggccg
     1141 tctcgagatg gagatgacag tcgctcccgg cgtcgggtat cggagcgcgg acgggaacaa
     1201 gggagccagc tcggtgatcg gggtgatccc ggtcgactcg atcttctccc cggtgcgcaa
     1261 ggtctcctac aaggtggaga acactcaggt cggccagatg accaacttcg accgtctggt
     1321 gctcgacatc gagaccaatg gcgccgtcga gcccggcgag gccgtgtctt cggcgggcaa
     1381 gacgctgcgg gagctgatgg gtctcttcgc cgacatcggt gaaggtgtcg gtctcgagct
     1441 gggcgaggtc cagcccgagt tctcgggctc accggacctc gacctcccga tcgaggccct
     1501 ggatctctcc gagcggcctc gcaactgcct gcgtcgggcc cagatcaaga ctgtcggcga
     1561 gctggtcgag cgcacttcgg acgatctgct caacatcacc aactttggcc agaagagcct
     1621 cgaggaggtc accgccaagc tcgacgagct cgggctcagc ctcgccgatg ccggcggcgg
     1681 cggttcctga tgccacgccc caagaagggg cccaggtttg gcgggtctcc atcgcacaac
     1741 aagaagatca tggccaatct ggccaccgag ctgtttctcc acgagaaggt gaccaccacc
     1801 ctgccccggg cccaagcggt gagaccgctg gccgagaagc tgatcaccaa ggctcgcaag
     1861 ggcgatctcc acgccaagcg gatcgtgctg aggacgattc ccaaccggga cgcagtggtg
     1921 aagctcttcg acgacgtggc accccgctac gccgatcgtc agggtggcta cacgcgaatc
     1981 acgaagcttg gcccgcgtcg cggtgatggg gccgaggaag cgatcatcga gctcgtctga
     2041 tcggggctcg cccccgagcc gtctacgacc agattcgctg ggcgaccac
//
LOCUS       DATITW010000725         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_35828, whole genome shotgun sequence.
ACCESSION   DATITW010000725 DATITW010000000
VERSION     DATITW010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35828"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VLS86_10085"
     CDS             complement(<1..363)
                     /locus_tag="VLS86_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HSO50883.1"
                     /translation="MFERFTDRARRVVVLAQEEARLLNHNYIGTEHILLGLLNEGEGI
                     AAQALESLDIDLASVREEVVKIIGQGQQSPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIHEGEGVAA"
     gene            464..1453
                     /locus_tag="VLS86_10090"
     CDS             464..1453
                     /locus_tag="VLS86_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSO50884.1"
                     /translation="MEDLQSLIRIPIVWDSLARVEERLLEAAAAEDPYLTKIAQHLLL
                     AGGKRFRPLLALLAAELGPSRDHRPVEAGVSVELIHVGSLYHDDVIDEADVRRGAASV
                     NANWSNTVAILAGDFLMARASEVAATHLSQESVRLLAATYADLVQGQTRELQLDFDLD
                     HGVDEYEEVIAGKTASLIRTSARLGAMAADAPDDVVEALSDWAWEVGMVFQIADDALD
                     LVADEETIGKPAGSDIREGKFTMPVLEALTGSDGPRVRELLQTAHPYPDDVIVEVIEI
                     VRSGGHVDTALSEATRRLDVATKALTGLPDGHPRQVLESLGSYLLQRVEAARR"
     gene            complement(1470..>2582)
                     /gene="lysS"
                     /locus_tag="VLS86_10095"
     CDS             complement(1470..>2582)
                     /gene="lysS"
                     /locus_tag="VLS86_10095"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HSO50885.1"
                     /translation="GELSVSVEEFSVLQKSLRPLPEKWHGLHDVELRSRRRYLDLMVN
                     DEARDVAIKRAAIVSELRRQFESRGFVEVDTPVLLRQATGATARPFATHHHALDMEMF
                     LRIATELYLKRLVVGGFEKVFEIGRIFRNEGLDSTHNPEFTRLEAYQALADYSDVMVL
                     VEEVFVGVTETVLGSTKVTFQGRELDLSPPFLRASMTSLVSERLGQEVTLDTGIPTLL
                     ALARAAGVEVEDGWSAGRVIAELYEELVESDIWQPTFVTHPPIEVSPLARRNPGDPRL
                     ADRFELVIAGSEYANAFSELNDPDDQRERFLTQAAARDAGDHEAHPVDEDYLLALEYG
                     LPPTGGLGIGVDRLVMLLTDQPHIREVILFPTMRSE"
BASE COUNT          444 a          857 c          817 g          464 t
ORIGIN      
        1 ggcggcgacg ccctcgccct cgtggatcag gccgaggagg atgtgctcgg tcccgatgta
       61 gttgtgcccc aactggagcg cctcgcgcaa gctgagctcg aggaccttct tggcccgtgg
      121 ggtgaacggg atgtgacctg aaggcgactg ctggccctgg ccgatgatct tgactacctc
      181 ttcgcgcacc gacgccaggt cgatgtcgag gctctccagt gcctgggcgg cgatcccctc
      241 accctcgttc aggagcccga ggaggatgtg ctcggtgccg atgtaattgt gatttaggag
      301 acgtgcctct tcttgggcca ggactacgac ccgtcgcgca cggtctgtga agcgttcaaa
      361 cactttcgac tcctgaccct gtcatacgca agtttaccac cggcgtgttc ctgaccttcg
      421 ggcatgcctg ctcatactcg ctcacgtaat accatcgtca cgaatggaag acctccaaag
      481 tctcattcgt attcccattg tctgggattc gttggcacgt gtcgaggaga ggctgctcga
      541 ggccgccgcc gccgaagacc cctatctgac caagatcgca caacacctcc tcctggcggg
      601 cggcaagcgc ttccgaccac tcctggccct gctcgccgcc gagctcgggc cgagccggga
      661 tcatcgcccg gtcgaggccg gggtctcggt ggagctgatc catgtcgggt cgctgtatca
      721 cgacgatgtc atcgacgagg cggacgtccg acgcggggcg gcgtcggtca acgccaattg
      781 gagcaacacc gtcgccatcc tcgccggcga cttcctcatg gccagggcct ccgaggtggc
      841 ggccacccac ctcagccagg agtcggtgag gctgctcgcc gccacctacg ccgatctggt
      901 ccagggtcaa acccgtgagc tgcaactcga cttcgatctc gaccatggcg tcgacgagta
      961 cgaggaggtg atcgcaggca agaccgcctc tttgatccgg acctcggctc gtctcggggc
     1021 catggccgcg gacgctcccg acgacgtggt ggaggcgttg tcggattggg cctgggaggt
     1081 ggggatggtg ttccagatcg ccgacgacgc cctcgatctc gtcgccgacg aggagacgat
     1141 cggcaagcct gccggatcgg acatccgcga gggcaagttc accatgccgg tgctcgaggc
     1201 cttgaccggc agcgacgggc ccagagtccg cgagctgctg cagaccgccc atccctatcc
     1261 ggacgacgtg attgtcgagg tcatcgagat agtccggtcg ggtggccacg tggacaccgc
     1321 cctctccgag gccacgcggc ggctcgatgt ggcgaccaag gccctgaccg gtctccccga
     1381 cggccacccg agacaggttc tggagagcct cggctcctac ctcctgcaac gggtcgaggc
     1441 agctcggcgc tagcccacct cggccaccgt cattcggatc gcatcgtcgg gaacaggatc
     1501 acctcgcgaa tgtgtggctg gtcggtgagc agcatgacca gccggtcgac cccgatgcca
     1561 aggccaccgg tcgggggcag gccgtactcc aacgccagca ggtagtcctc gtccaccgga
     1621 tgagcctcat ggtcaccggc gtcccgtgcc gcagcctggg tcaggaacct ctcacgctgg
     1681 tcgtcggggt cgttcaactc gctgaacgca ttggcgtact cggagccggc gatcaccaac
     1741 tcgaagcgat cggcaagtcg tggatcgccc gggttgcgtc gagccagcgg agagacttcg
     1801 atcggggggt gggtgacaaa ggtcggctgc cagatgtcgc tctcgaccaa ttcctcgtac
     1861 aactcggcga tgacacggcc ggcgctccac ccgtcctcca cctccacgcc cgctgcccgg
     1921 gccagggcca gcagcgtcgg aatcccggtg tcgagggtga cctcctgacc cagccgctcc
     1981 gagaccaggc tggtcatcga cgcccggagg aatggtgggg acaaatcgag ctcgcgcccc
     2041 tgaaaggtca ctttggtcga cccgagcacg gtctcggtga ccccgacgaa gacctcctcg
     2101 accaggacca tcacgtctga gtagtcggcc agggcttgat aggcctcgag cctcgtgaac
     2161 tcggggttgt gcgtcgagtc gagtccctcg ttgcggaaga tccgaccgat ctcgaacacc
     2221 ttttcgaaac ctcccaccac cagtcgcttc aagtagagct cggtggcgat gcgcaagaac
     2281 atctccatgt ccagggcgtg atggtgggtg gcgaagggcc gggcggtagc ccctgtcgcc
     2341 tgtcgcagca gcaccggggt gtccacctcg acgaatcccc tcgattcgaa ttggcgacgc
     2401 aactcggaga cgatcgcggc gcgcttgatc gcgacgtccc tcgcctcgtc gttgaccatc
     2461 agatcgaggt atcgcctccg actcctgagc tcgacgtcgt ggagaccgtg ccatttctcg
     2521 gggagaggtc gaaggctctt ctggagcacg ctgaactcct cgacactaac gctgagctca
     2581 cc
//
LOCUS       DATITW010000726         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_676000, whole genome shotgun sequence.
ACCESSION   DATITW010000726 DATITW010000000
VERSION     DATITW010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676000"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VLS86_10100"
     CDS             complement(<1..552)
                     /locus_tag="VLS86_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HSO50886.1"
                     /translation="MRIAFAGNRSVARRCLQHLIEVGVKPMALLIPTGDETELQELAG
                     LGPEHILVGGEFRESGGIELLRSLDLDYILGVHFPYIVPEAVLGIPRIGALNLHPALL
                     PHNRGWHTASWAILEGTPIGATLHFMDRGVDTGDIVAQVELEVRPDDTAHTLYARLLD
                     LEVELFRSAWPLLASGDPPRMTQS"
     gene            complement(552..1145)
                     /locus_tag="VLS86_10105"
     CDS             complement(552..1145)
                     /locus_tag="VLS86_10105"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HSO50887.1"
                     /translation="MKRALDAAVSALALVILSPLLLVIAALVRWRLGSPVLFRQERPG
                     LHAKPFTLVKFRTMSLQPADPTDGSGDQARLDPFGAKLRSTSLDELPELWNVLKGDMS
                     LVGPRPLLMHYLPLYSERQARRHEVRPGVTGWAQINGRNATPWPERLEMDVWYVENRS
                     MWLDLKILAMTVPSALRRSGVTQEGQATVEYFTGSET"
     gene            complement(1142..>2080)
                     /locus_tag="VLS86_10110"
     CDS             complement(1142..>2080)
                     /locus_tag="VLS86_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50888.1"
                     /translation="GFVLFAMISYLGQLGLYAAFRRWAKPHQLKPYAFLVLFLPTYAF
                     WPSSIGKDALVVLFLGLSAYSISRLLESFELRWVFPVALALGGLGLIRIHVAALVALA
                     LVGAVIVSKLKIGAGFLVKGRKFITFAGAAAAVVLAVTLIPDIVGLELSSADDIAPFT
                     EEITRRTSEDGTVASGNPVRSIVDVPAALALVLFRPFIFEASQVQHLLAAAETTLVLL
                     LTVWKLPAMLRNWGQWRRNAYLVFCTFYTVGFAIVFSVVRNLGIIARQRGQVLAFFLA
                     VVIGLGWNESRKAIRERAESALLTPETELTGTAIDR"
BASE COUNT          395 a          658 c          706 g          321 t
ORIGIN      
        1 tgattgggtc atccggggcg ggtcaccgga ggccaggagc ggccaggcac tgcgaaacag
       61 ctcgacctcg aggtcgagca ggcgggcata gagggtgtgt gccgtgtcat cggggcgaac
      121 ctcgagctcg acctgggcca cgatgtcccc ggtgtccaca ccgcggtcca tgaaatggag
      181 tgtggctccg atcggtgtgc cctcgaggat cgcccagctc gctgtgtgcc acccccgatt
      241 gtgggggagg agggcagggt gaaggttgag cgccccgatc ctcgggatcc ccaggaccgc
      301 ctcggggacg atgtagggaa aatgcacccc gaggatgtag tcgagatcga gggaacgcag
      361 gagctcgatg ccaccactct cgcgaaactc tcccccgacc aggatgtgct cggggcccaa
      421 ccccgccagt tcctgcagct cggtctcgtc gccggtgggg atgagcaagg ccatgggctt
      481 caccccaact tcgatcaggt gctggagaca cctcctggcc acgctcctgt tgcctgcgaa
      541 cgcgattctc atcacgtctc cgagccggtg aagtactcga cggtcgcctg accctcttgg
      601 gtgacaccac tccgacgcaa agcgctgggc accgtcatgg cgaggatctt gaggtcgagc
      661 cacatcgatc ggttctccac gtaccagaca tccatctcca gacgctccgg ccagggtgtc
      721 gcatttcgac cgttgatctg cgcccacccg gtgacccccg gtctcacctc atggcggcgc
      781 gcctgtcgct cggagtacag cggcaggtag tgcatgagaa gggggcgggg accgaccagg
      841 ctcatgtcgc ctttgaggac gttccacagc tccggaagct catcgaggct cgtgcttctg
      901 agcttggccc cgaacgggtc gagtcgagcc tggtcgccgg agccgtcggt cgggtcggcc
      961 ggctggaggc tcatggttcg gaacttgacc agggtgaacg gcttggcgtg gagaccgggg
     1021 cgctcctgtc ggaacagcac gggactgccc aatctccatc gaacgagagc ggcgatgacg
     1081 agcagcagtg gtgagaggat caccagcgcc agcgccgaga cggctgcgtc cagggcgcgt
     1141 ttcaccgatc gatagccgtc ccggtgagct cggtctccgg cgtcagaagc gcgctctccg
     1201 cgcgttcccg gattgccttc cggctctcgt tccagcccag accgatgacc accgccagga
     1261 agaacgcaag cacctgtccc cgctgacggg cgatgatccc aaggttgcga acgacactga
     1321 acacgatggc gaagccgacg gtgtaaaagg tgcagaagac cagataggcg ttacgtcgcc
     1381 actgccccca gtttcggagc atggcgggga gcttccacac cgtgagcaac aggaccaggg
     1441 tggtctcggc ggcggcgagc aggtgctgca cctgcgacgc ttcgaagatg aacggccgga
     1501 acagcaccag ggccagcgcc gcgggcacat cgacgatgga gcgaaccgga ttccccgagg
     1561 cgacggtccc gtcctctgag gtgcgtcttg tgatctcctc ggtgaacggg gcgatgtcgt
     1621 cggcgctgct gagctcgagc ccgacgatgt cagggatgag ggtcaccgcg aggaccaccg
     1681 ctgccgccgc cccggcgaag gtgatgaact ttcggccctt caccaggaag ccggcgccga
     1741 tcttgagctt ggagacgatg accgcaccga ccagggcgag ggcgaccagt gccgcgacat
     1801 ggatcctgat caggcccagg cctcccagcg ccagggccac cgggaacacc cagcgcaact
     1861 cgaagctctc cagcagcctg gagatggagt aagccgacag cccgaggaag agcacgacga
     1921 gcgcgtcctt gccgatgctc gacggccaga aggcataggt gggcaggaag aggacgagga
     1981 aggcgtacgg cttgagctgg tggggcttgg cccaccggcg gaacgcggcg tagaggccga
     2041 gctgacccag atacgagatc atggcgaaca gcacgaaccc
//
LOCUS       DATITW010000727         1605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_355710, whole genome shotgun sequence.
ACCESSION   DATITW010000727 DATITW010000000
VERSION     DATITW010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355710"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..522
                     /locus_tag="VLS86_10115"
     CDS             157..522
                     /locus_tag="VLS86_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019525264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSO50889.1"
                     /translation="MSPYFESYLTVGAFAVLGAILVMVMLGVASVLRPTNPTTTKRLT
                     YECGVDPVGSGWSQSYVRYYVYGLLFVIFDVEAVFIFPWAIVIEQLGTFGLVEMFIFI
                     DILILGLVYAIRKGVLRWE"
     gene            513..1010
                     /locus_tag="VLS86_10120"
     CDS             513..1010
                     /locus_tag="VLS86_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSO50890.1"
                     /translation="MGVVQKFGAPKPVSWLLNWSRKYSLWLFQFGLACSAIELMAASG
                     PRYDFMRFGIIPLPASPRQADLMVVAGTVTDKMAPAVRRLYDQMPEPKYVISMGSCSN
                     CGGPYWDSYSVTKGVDQVIPVDVYVPGCPPRPEALMDGIVLLQKKIQGEDIKEKWNQR
                     DLEPV"
     gene            991..1596
                     /locus_tag="VLS86_10125"
     CDS             991..1596
                     /locus_tag="VLS86_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSO50891.1"
                     /translation="MTSSPSETETTVHPLQEFADRVAEAVGGVAEITFDTVKVKVPVE
                     RWVEIHRQARDDLGLVFFSWLSAIDWTVEVAVGDPPSGEVVDRFEMLTTVGDLGEGKR
                     VTFTADLSKDNPVIASLVEIYAGATWHEREAAEMFGIQFEGHPDLSHLYLVDSFIGNP
                     LRKSYPLLSREVKPWPGTVDVEGMPGSAAEATEPSEENPEA"
BASE COUNT          281 a          493 c          516 g          315 t
ORIGIN      
        1 cagaccagcg gagtctggag agggggcgtc cgccatcgat ccaggctagg gccggctcca
       61 cggtttttgc cgatggcagt tggagtttcc aaacttcgac ggaaaaggac ccgtgcgtat
      121 actcgcccgc ggtggtgtcc atcgacccgc agaggcatga gcccttactt tgagagctat
      181 ctgacggtcg gcgctttcgc cgtactgggc gccatcctgg tgatggtgat gctcggggtc
      241 gcctctgtgc ttcggcccac caaccccacc acgaccaaac gcctcaccta tgagtgcggc
      301 gtcgacccgg tcggctcagg atggagccag agctatgtcc gctactacgt ctacgggctt
      361 ctcttcgtaa tcttcgacgt ggaggctgtg ttcatcttcc cctgggcgat cgtgatcgag
      421 cagttgggca ccttcggtct ggtcgagatg ttcatcttca tcgacatcct gatcctgggt
      481 ctggtctatg ccatccgcaa gggagtcctg cggtgggagt agtccagaag ttcggggccc
      541 cgaagccggt gagctggctt ctcaattggt cccggaaata ctccctgtgg ctcttccagt
      601 tcggcctcgc ctgcagcgcc atcgagctga tggctgcctc gggcccgcgc tacgacttca
      661 tgcggttcgg gatcatcccg ctgcccgcct cgccgaggca ggcagaccta atggtggtgg
      721 ccggcactgt gaccgacaag atggcgccgg cagtgcgacg gctctacgac cagatgcctg
      781 agccgaaata cgtgatatcg atgggctcct gctccaactg cggcggcccg tattgggatt
      841 cgtactcggt gaccaagggt gtcgaccagg tgatcccggt cgatgtctac gttccaggtt
      901 gcccgccccg gcccgaggcg ctgatggacg ggatcgtcct cctccagaag aagatccagg
      961 gcgaggacat caaagagaag tggaaccagc gtgacctcga gcccgtctga gaccgagacg
     1021 acggtccatc ctcttcagga gttcgccgac cgggtcgcgg aggcggttgg gggcgtggcc
     1081 gagatcacct tcgacacggt aaaggtgaaa gttcccgtcg aacgctgggt ggagatccac
     1141 cgccaggccc gggacgatct cgggcttgtt ttcttctcat ggctgtccgc gatcgactgg
     1201 acggtcgagg tcgcggttgg agacccgccc agcggtgagg tggtggaccg tttcgagatg
     1261 ttgaccaccg tcggtgacct cggcgagggg aagcgggtca ctttcaccgc cgacctctcc
     1321 aaagacaacc cggtgatcgc cagcctggtc gaaatctacg ccggcgccac ctggcacgag
     1381 cgcgaggcgg ccgagatgtt cgggatccag ttcgaaggtc accccgacct ctctcatctc
     1441 tacctcgtcg actccttcat cggcaacccg ctgcgcaaga gctacccgct gctcagtcgt
     1501 gaggtgaagc catggccggg gacggtggac gtggaaggaa tgccgggatc cgcggccgag
     1561 gccaccgagc catctgaaga gaaccctgag gcctgaagtg gtcgc
//
LOCUS       DATITW010000728         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_676133, whole genome shotgun sequence.
ACCESSION   DATITW010000728 DATITW010000000
VERSION     DATITW010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676133"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VLS86_10130"
     CDS             <1..291
                     /locus_tag="VLS86_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50892.1"
                     /translation="VLTMLEGILVVAAPLWLLDLFRRRYGHRGRLALLMSRSAFAAFV
                     MHQLVLVGLVLASRLIPWPPEVEYLLVSGLGVAVSFGLGALLVRVPGVNRFV"
     gene            317..1012
                     /locus_tag="VLS86_10135"
     CDS             317..1012
                     /locus_tag="VLS86_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HSO50893.1"
                     /translation="MSAGRIGRWWSVAAAKLRHMEHESRLDHDGSAGSAAIPLGAALA
                     ASVGPRAAVLVEGVSDQAAIETLARRRGRDLGTEGVAVVPIGGASKIGHFLTLLGPRG
                     FDIRLAGLCDVAEQGSFLRGLERAGIGSDLTRSGMESLGFHVCVVDLEDELIRTLGPG
                     TVEQLIDAQGELGSFRTMQRQPAQRDRPVHDQLRRFMGTRSGRKILYARLLVEALDLE
                     RVPRPLDGVLEAV"
     gene            complement(1026..1379)
                     /locus_tag="VLS86_10140"
     CDS             complement(1026..1379)
                     /locus_tag="VLS86_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50894.1"
                     /translation="MSERPPQPEEALGPEELQRFMYACSHERRSHTTHRLIDAASYGW
                     IHMFIRHHGEEAPPRESGDDWLAPHDADPAKIAAFFGRVAIRLRNTGAFDYATTLETY
                     FEELAEAYQAPWPQG"
     gene            complement(1450..1848)
                     /locus_tag="VLS86_10145"
     CDS             complement(1450..1848)
                     /locus_tag="VLS86_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50895.1"
                     /translation="MTETITTTAASLFLDDHGLLHIASNGVPSTKKTVTETFAAARTL
                     APHPVPTLFDARLWPIGGADFWVTFIDLLPSVVSAGAILVAPDQATGLGGFPREVNRL
                     MIPFEVFTVEDEAIEFLTPFVRPIPEDEEE"
     gene            complement(1909..2085)
                     /locus_tag="VLS86_10150"
     CDS             complement(1909..2085)
                     /locus_tag="VLS86_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50896.1"
                     /translation="MRLATGIILLVAGTVWTLQGLDVFFAPKSFMSGDRAWVWFGILA
                     VAVGGGLAWTARPK"
BASE COUNT          347 a          677 c          726 g          414 t
ORIGIN      
        1 gtcctgacca tgctcgaggg gatactggtg gtggccgccc ctctgtggtt gctcgatctc
       61 tttcggcggc gatacggaca tcgaggtcgg ctcgccctcc tcatgagtcg gtcggcgttc
      121 gccgcgttcg tcatgcacca gctggtactg gtcgggttgg tcctggccag ccggctgatc
      181 ccatggccgc cagaggtgga gtacctgttg gtgagcgggc tcggcgtggc tgtttcgttt
      241 ggtctcggtg ccctcctggt acgggtgccc ggtgtgaacc ggttcgtctg accgcggtcc
      301 cacccggcgc caatcggtgt ccgcgggccg aatcggtcgc tggtggagcg ttgccgctgc
      361 caagctgcgg cacatggagc acgaatcacg actcgatcat gacgggtccg cggggtctgc
      421 ggccatccct ctcggcgccg ccctggccgc cagcgtgggc ccgcgcgcgg ccgtgctcgt
      481 ggaaggggtc agtgaccaag cggcgatcga gaccctggcc agacgtcgcg gtcgtgatct
      541 gggcaccgaa ggcgttgccg tggtccccat cggtggtgcg tccaagatcg ggcactttct
      601 gaccctgttg ggcccgcggg gattcgacat caggttggcc ggtctctgcg atgtcgcaga
      661 gcaaggcagc tttcttcgcg gtctggagcg ggccggaatc gggtccgatc taacgcggtc
      721 cgggatggag tcgctcggat tccacgtctg cgtcgtcgac ctggaggacg agctcatccg
      781 gaccctcggg cctggcaccg tggagcaact gatcgacgct cagggagagc tcgggtcgtt
      841 ccgcaccatg cagaggcaac cggcgcaacg agatcggccc gtgcatgacc agttgcgacg
      901 tttcatgggg acccgttccg ggaggaagat cctctacgcc cgtctgctcg tggaggctct
      961 cgatctggag cgggttcctc gacctctgga tggggtgctc gaggcagtgt gaccgctctc
     1021 cggcctcacc cctggggcca gggcgcctgg taggcctcgg ccaactcctc gaaataggtc
     1081 tcgagtgtcg tggcgtagtc gaaggcgccg gtgttcctga gcctgatggc gacccgcccg
     1141 aagaaggcgg cgatcttcgc cgggtcggcg tcgtgaggag ccagccagtc atcaccagac
     1201 tcccgtgggg gagcctcctc cccatggtgg cggatgaaca tgtggatcca tccataggag
     1261 gccgcgtcga tcagacgatg ggtggtgtga gatcgccgct cgtgtgagca ggcatacatg
     1321 aagcgctgaa gctcctcggg ccccagggct tcctctggct gaggtggtcg ttcgctcatc
     1381 aggaggagtc ccgaacggag tctaagaccg gaggggccgg cgcccccggc tatccagagc
     1441 tttcctgcgt cattcctctt cgtcctcggg gatgggtcgg acgaacggag tcaggaactc
     1501 gattgcctcg tcttcaaccg tgaacacctc gaaggggatc atcaggcgat tgacttcccg
     1561 cgggaaccca cccaatcccg tcgcctgatc cggggcgacg aggattgctc cggcgctgac
     1621 cacggagggc agaagatcga tgaaggtcac ccagaagtcc gcacccccga ttggccacaa
     1681 tcgagcatcg aacagggtgg ggacggggtg cggggccagg gtgcgtgctg cggcgaaggt
     1741 ctcggtcacg gtcttcttgg tgctcgggac tccattgctc gcgatgtgga gcagaccgtg
     1801 gtcatcgagg aagagcgacg ccgcggtggt tgtgatcgtc tcggtcatgt tggcccccgt
     1861 tgtctcgaag cctatccaag ttcttccata ccgtgggtgg cttgtccact atttgggacg
     1921 agccgtccag gccagcccgc cacccactgc caccgccaag atcccaaacc aaacccaggc
     1981 tcggtcgccc gacatgaagc tcttcggggc gaagaacaca tcgaggccct gcagggtcca
     2041 gaccgtccct gccacaagca ggatgatccc tgtggcgagc ctcatttgcc tcacctcgcc
     2101 aacctagctc ttgacatagt gcgccattca gcgaataatc ccgcagacat gacgacgagc
     2161 ctgc
//
LOCUS       DATITW010000729         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_596657, whole genome shotgun sequence.
ACCESSION   DATITW010000729 DATITW010000000
VERSION     DATITW010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596657"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..815
                     /locus_tag="VLS86_10155"
     CDS             327..815
                     /locus_tag="VLS86_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50897.1"
                     /translation="MSESRRGADEVVPVPEAPPSRGEAYVPSREPSGAAVGWITFAGV
                     VMIVEGSFEILAGLAALINPDSFTGVDTIFEQNAETWGWWHLVIGGVILLSGFGVFTG
                     NVLARTVGVIAASISAIAAFVWLPFYPVWGIIVIAIDIAVIWALTVHGRDIQKAERMG
                     EF"
     gene            complement(1185..1520)
                     /locus_tag="VLS86_10160"
     CDS             complement(1185..1520)
                     /locus_tag="VLS86_10160"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HSO50898.1"
                     /translation="MDRFFTAFSTLVAKWTGSHWTFAIVAVLVVVSLLTIGVEDTNIA
                     ISIATLLMVFVLQNTQNRDSAALHLKLDEMIHAEPDAREIVRGIEVKSEAEIKGMTLE
                     ETDSVTPGE"
BASE COUNT          303 a          537 c          542 g          327 t
ORIGIN      
        1 gtccgcgttc tcctgatcct gcacgtcgag atcggcctgg tgcgccttca gcagatcctc
       61 gggcgtgatg cccttcatgt tgcgatggac gtccatgaac tccggcatgc tcgctcctct
      121 cgttggtcga tccattgtgt acgccagccg tcggtccctc gcaacctggc gacgatcgtc
      181 tcaccacgat gagcgggtgc gtgcaggtcc accgcaccgc ctggagcggg tttgcgtagg
      241 tcgttttact gactcgaacg gaggtaggga cgaggtagta cggtggtcac ggccgcgatg
      301 gccgagacct ggaggaggga gaggccatgt cagagagtcg cagaggggcc gatgaggttg
      361 tgcctgttcc cgaggcgccg ccgtcacgag gggaggcata cgtcccgagc agagagccga
      421 gcggcgcggc cgtcgggtgg atcactttcg ccggggtggt catgatcgtc gaaggctcgt
      481 tcgaaatcct ggcgggcctg gccgcactga tcaaccccga ctcgttcacg ggcgtcgaca
      541 ccatcttcga gcagaacgcg gagacctggg gatggtggca cctggtgatc ggcggggtca
      601 tactcctgtc cggcttcggc gtgttcacgg gcaacgtcct ggctcggacc gtcggcgtga
      661 tcgcggcatc gatcagcgcc atcgcggcct tcgtgtggct gccgttctac ccggtctggg
      721 gcatcatcgt gatcgcgatc gacatcgccg tgatctgggc gctgaccgtc cacggtcgcg
      781 acatccagaa ggccgagcgg atgggcgagt tctagccacc gggacatcca ggctctatcc
      841 aatacgaggc tgttcccaag ggacctccca cccgcaccct gtttgcgtta tcggcaatgg
      901 agactccttg atggggcaac cccagaaccc gcacattcgg cgaactgctg atcgactgcg
      961 aggaggaccg gatccttcgg gggagcgcgg ttcgcggtag cgaagaggct ggggccggat
     1021 tcccggcctc tcgcactcta tgggttaggc gatgccgctc cacggcgagt cgcgcacgac
     1081 ggatccctgt cccgatagct ctacgcgtct cgatgcacga accacagacg aagtgccgtc
     1141 tcttcgcacg atgttcgagc gacaccgaga ctgagactga ggggctactc tccgggcgtt
     1201 accgagtccg tctcctccag cgtcatgccc ttgatctcgg cttcggactt gacctcgatg
     1261 ccgcgcacga tctcacgtgc atcgggttcg gcatggatca tctcgtctag cttgagatgc
     1321 agcgcggcgg agtctcggtt ttgggtgttc tgaaggacga acaccatcag cagggtcgcg
     1381 atggagatcg cgatgttggt gtcctccacg ccgatggtga gcaggctcac caccacgagc
     1441 accgcgacga tcgcgaaggt ccagtgactc ccggtccact tcgccaccaa ggtcgagaag
     1501 gcagtgaaga accgatccat cccgccctca ctattgctcc cgctgttcgg atgagtcacc
     1561 ccggttccca ccttccctgg acggcattcc ggccaaaggg tctagcgccg aacgaagtag
     1621 aggatcacga ggacaacaat caccacacga cgatgccaac gctcccgata cctccaagag
     1681 cggcgatcaa tggggcctcc cttctcgga
//
LOCUS       DATITW010000730         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_195746, whole genome shotgun sequence.
ACCESSION   DATITW010000730 DATITW010000000
VERSION     DATITW010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195746"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VLS86_10165"
     CDS             <1..353
                     /locus_tag="VLS86_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSO50899.1"
                     /translation="RLAAEHLKMLQDCAPNLTDEKILARKAKSPVDIEASNPHMWRGT
                     IHGGDRGLAYGGAQRPVPGWAQHRMPIPGLYQTGATTHPGGSITGGPGRNAATVILDD
                     LGTTIEAVVAEQER"
     gene            406..1539
                     /locus_tag="VLS86_10170"
     CDS             406..1539
                     /locus_tag="VLS86_10170"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO50900.1"
                     /translation="MTLELAFAPNEDPFVLAHGGFYRRWFSMIDDLAELQATVARLEG
                     TTQDKWVPVWEEVARRHEAEGDRLEEAGDHQGSRRSFLQAKTYYAIGRFPGEVSDVKR
                     RISEDCARAYRKACAHLDPPMEVVEFDHQGKTIRAHFRGPHTDSLGPAVLVMCGADVF
                     KEDRGWAQDLCIDNGMAALVMDGPGTGENPFPWAPESVDAWEAAVDYLADRPEVDRNR
                     IGAFGISRGGYSVMQLAGTAPDKLKAVVANAGHPFGYVMGDDELEEFVRVRNERATWR
                     FGAPDDPPSFPPTSVEEERSEFSRWALSEQGILDNITMPVLMINGKHDHLSPIGNIYF
                     MLETGPATGKEARIYSDGGHCAFKYFDHWAPATFHWLAEKLAR"
BASE COUNT          287 a          510 c          500 g          260 t
ORIGIN      
        1 accggctcgc cgccgagcac ctgaagatgc ttcaggattg cgccccgaac ctgaccgatg
       61 aaaagatctt ggcccgcaaa gcaaagagcc ccgtcgacat cgaggcctcc aacccgcaca
      121 tgtggcgagg gaccattcac ggtggtgacc gcgggttggc ctacggagga gcgcagcgcc
      181 ccgtcccggg ctgggcccag caccggatgc cgatcccggg cctgtaccaa acgggagcca
      241 cgacccatcc aggtgggtcg atcaccggtg gccccggtcg caacgcggcg acggtgatcc
      301 tcgacgacct ggggacgacc atcgaggctg tggtcgcgga gcaggaacgg tgacggtaaa
      361 cgatcccgtg gctagcgtca cccgacccga tcaggagagc atgcattgac actcgaactc
      421 gctttcgccc ccaacgaaga cccattcgtt ttggcccacg gcgggttcta tcgccgctgg
      481 ttctcgatga tcgacgacct cgctgagctc caagcgaccg tggcacgtct cgagggcacg
      541 acccaagaca agtgggtccc ggtgtgggag gaggtcgccc gtcgccatga ggcggagggg
      601 gatcggctgg aggaggcggg tgaccaccag gggtcacgac gctccttcct ccaggccaaa
      661 acctattacg ccattgggcg ctttccggga gaagtgtcgg atgtcaagcg gcggatcagc
      721 gaggactgcg cacgcgccta ccgaaaggcc tgcgctcatc tcgacccacc tatggaggtc
      781 gtcgagttcg accaccaggg caagacgatc cgggcccatt ttcgtggtcc acacaccgac
      841 tcgctcggtc ccgccgtcct cgtcatgtgt ggcgccgacg tcttcaagga ggatcggggc
      901 tgggcccagg acctgtgcat cgacaacggg atggccgccc tggtaatgga tggccccggc
      961 accggagaga acccgttccc atgggcgccc gagtcggtgg acgcctggga ggcggccgtc
     1021 gactatctgg ccgaccgacc cgaggtcgat cggaaccgga tcggggcctt cggcatcagt
     1081 cggggcggct attcggtcat gcaattggcc ggcactgctc ctgacaagtt gaaggcagtg
     1141 gtggcgaacg ccggtcatcc cttcgggtac gtgatgggcg acgacgagct cgaggagttc
     1201 gtccgtgtcc gcaacgagcg cgcgacctgg cgcttcggcg ccccggacga tcccccttct
     1261 ttccctccga catcggttga ggaggagcgc tccgagttct cccgctgggc tctgtcggag
     1321 cagggaatcc tcgacaacat caccatgccg gtgctgatga tcaacggcaa gcatgatcac
     1381 ctgtccccga tcggcaacat ctatttcatg ctcgagaccg gccccgccac cggtaaggag
     1441 gcccgcatct actccgacgg cggtcactgt gctttcaaat acttcgacca ttgggcgccg
     1501 gccaccttcc actggctcgc cgagaaattg gcacggtgac catcgggaca tcgctcg
//
LOCUS       DATITW010000731         1668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_116462, whole genome shotgun sequence.
ACCESSION   DATITW010000731 DATITW010000000
VERSION     DATITW010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116462"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VLS86_10175"
     CDS             complement(<1..257)
                     /locus_tag="VLS86_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020027749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO50901.1"
                     /translation="MSKIFPPPQKDGEPVTAIGDITLEVEGNVFVSIVGPSGCGKSTL
                     LNILSGVETPTSGTVSVTEGGRPARLGYVFQDARLLPWRTV"
     gene            complement(283..1155)
                     /locus_tag="VLS86_10180"
     CDS             complement(283..1155)
                     /locus_tag="VLS86_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSO50902.1"
                     /translation="MTIAPVVIPEAPSEPGRLRQILTSPQGAQVTAIVVFIGAWQLLT
                     LVAPRVPGPVEVTQFLYTEITGGSHGGIITGEFFEHFAITLRRFSFGLLLAFVPGLLI
                     GVLIGSSRIASAFLNDTVLVLLTMPAFVWAFLTTMWFGISWRAPVLTVAFAAFPFVAI
                     NVAQGVRSITPDLKNMSSAFDVGRWKRFRHLTLAAVTGYIFAGLRFAIIVGWNGILLS
                     EWFSGQAGVGFRTRYWFDANRYRGFLGWVIVFIFFIVVLDRLVLNRLQQRAFRWRDPV
                     EVKVEVAIPEQAGI"
     gene            complement(1152..>1668)
                     /locus_tag="VLS86_10185"
     CDS             complement(1152..>1668)
                     /locus_tag="VLS86_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSO50903.1"
                     /translation="SKYWGNFLQSPVVVAGSIPGLTYAVMAFVVFGISFWGPVLAVGL
                     IAMPYVAINVAEGVRGVDRRLIDMSTAYGRNEAQVFRHVTVPSVLPFIFAGVRLSFSL
                     AWKVGTLTEVFGSSKGIGFQIRRNYQLFNMPAVIAWVLLFVAFMLLLERLVLVRIERR
                     LFRWRQWERDR"
BASE COUNT          362 a          532 c          537 g          237 t
ORIGIN      
        1 atcaccgttc gccaggggag gaggcgggca tcctggaaca catacccgag acgcgcaggc
       61 cggccgccct cggtgaccga gaccgtcccg ctggtcgggg tctcgacccc ggacaggatg
      121 ttgagcaggg tgctcttccc gcaccccgag ggcccgacga tcgagacgaa cacattccct
      181 tcgacctcga gcgtgatgtc gccgatcgcg gtcaccggct cgccgtcctt ctggggcggc
      241 gggaagatct tgctcaatcg ctgaatgtcc aaccgggcca tctcaaatac ctgcctgctc
      301 gggtatggcg acctcgacct tgacctcgac cgggtctctc catcggaacg cgcgctgctg
      361 aaggcgattc aagaccagcc gatccagaac cacgatgaag aatatgaaca cgatcaccca
      421 ccccaggaac ccccggtagc gattggcatc gaaccaatac cgggtgcgaa agccgactcc
      481 ggcctgcccg ctgaaccact cggagaggag gatcccgttc cagccgacga tgatggcgaa
      541 ccggagcccg gcgaagatgt atccggtgac cgcggccagg gtcaggtgtc ggaatcgctt
      601 ccaccggccc acgtcgaatg ccgacgacat gttcttgagg tcgggggtta tggagcggac
      661 accctgcgcg acgttgatgg cgacgaacgg gaaggcggcg aaggccacgg tgagcaccgg
      721 tgcccgccat gagatgccga accacatcgt ggtgaggaac gcccagacga aggccggcat
      781 ggtgagcaag acgagaacgg tgtcgttgag gaatgcgctg gcgatcctcg acgatccgat
      841 caggaccccg atgaggagcc cgggcacgaa cgcgagcagc aggccaaacg agaagcgtct
      901 gagcgttatc gcaaaatgct cgaagaactc accggtgatg atgcccccgt gcgacccgcc
      961 ggtgatctcg gtgtagagga actgcgtcac ctcgaccggg ccggggaccc ggggcgccac
     1021 cagtgtgagc aactgccagg ctccgatgaa gacaacgatc gcggtgacct gtgctccctg
     1081 ggggctggtc aggatctgcc ggagccggcc cggctcagag ggagcctcgg ggatcacgac
     1141 cggggcaatt gtcaccggtc acgctcccat tgacgccatc tgaagagacg acgctcgatt
     1201 cgcaccagga ccagacgctc gaggaggagc atgaaggcaa cgaagaggag cacccaggcg
     1261 atgaccgccg gcatgttgaa cagctggtag ttgcgccgga tctggaaccc gatgcccttg
     1321 gacgacccga agacctcggt gagagtcccc accttccacg ccagggagaa ggagagccgc
     1381 acgccggcga agatgaaagg aagcaccgac ggcaccgtca catggcggaa gacctgcgct
     1441 tcgttccgcc cgtaggcagt gctcatgtcg atgaggcgtc gatcgacccc gcgaaccccc
     1501 tcggctacgt tgatcgccac ataaggcatc gcgatcaggc ccacagccag caccgggccc
     1561 cagaacgaga tgccgaacac gacaaaggcc atcacggcat aggtgagccc ggggatcgac
     1621 cccgctacca cgaccgggga ctggaggaag ttgccccagt acttgctt
//
LOCUS       DATITW010000732         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_355978, whole genome shotgun sequence.
ACCESSION   DATITW010000732 DATITW010000000
VERSION     DATITW010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355978"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VLS86_10190"
     CDS             complement(<1..773)
                     /locus_tag="VLS86_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSO50904.1"
                     /translation="MSLPVRRPQFSPDTVYLDTPTYGLAASSVIAVMTEALDRWRRGI
                     ATMAEYDEAVAGSRRLFSEIVNVDPERVAVANQVSVFVGVVASSLADGNVVLAPEGDF
                     ASLMFPVLVQGRRGITLRTAPLSGLAEAVDADTDLVAFSLVQSADGVQADLDSILDAA
                     ARHGAATLVDATQAAGWLPIEASRFDNMVVSAYKWLLCPRGTAFMTLGAGHDPGMPPI
                     LAGWYSGEDPWESVYGTPLRLADSARRYDVSPAWLSWMGS"
     gene            858..1397
                     /locus_tag="VLS86_10195"
     CDS             858..1397
                     /locus_tag="VLS86_10195"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HSO50905.1"
                     /translation="MRFLAFFALIALAEVATFFWVGSKIGLLWALGLAITTALLGAFL
                     VRRAGLSVLGRIRERMDQGQLPGRELSDGAAILVAGAFLISPGFITDALGFLLLIPAV
                     RALIHRVLWIRLAGRMRVFTVNRQTMSGVWADRTGSSGGRRDDVIDIDDVELFPLADP
                     RDERANSPLPSGEPAEPGE"
     gene            complement(1368..>2163)
                     /locus_tag="VLS86_10200"
     CDS             complement(1368..>2163)
                     /locus_tag="VLS86_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSO50906.1"
                     /translation="LVAVVVATVAFARPVVAGETQESIQLVVLAIDTSISMEADDVTP
                     SRVDAARESAGAFLDTVPEGVAVGLVGFDGEARLLIAPTTNLEAVRRVVDSAIDRNGL
                     GEGTAIGEAVFLGIDAIESSAAQVDGAGEETGEAPGTIVVLSDGDTTMGRTNDEAASA
                     ARGAGIPVHTIAFGTDSGTIVDPYGEVIPVPVNAKALEDLARQTDGRPLTAATAEQLS
                     EVYADLGRSVQVETEPVEVGDWFVGAALVILALAGVGSLAWFGRLP"
BASE COUNT          352 a          745 c          712 g          354 t
ORIGIN      
        1 gagcccatcc acgacagcca ggcaggcgac acgtcatagc gacgagcgga gtcggccagc
       61 cgcagcggtg tcccgtagac ggattcccac ggatcctccc ccgaatacca ccccgccaga
      121 atcggcggca tgcccgggtc gtgacccgct ccgagggtca tgaacgcggt cccgcgtggg
      181 cacaacagcc acttgtaggc ggagaccacc atgttgtcga agcgagaagc ctcgatcggc
      241 agccatcctg ccgcctgggt ggcgtcgacc agtgtcgccg ccccgtgccg ggcggcggcg
      301 tcgagtatcg agtcgaggtc ggcctgaacg ccgtcggctg actgcacgag gctgaacgcc
      361 accagatcgg tgtcggcgtc gaccgcctcg gcgaggccgg acagcggcgc agtgcgcaag
      421 gtgatccctc gtcttccctg gaccagcacg gggaacatca gcgatgcgaa gtcaccctcg
      481 ggggcgagga caacatttcc gtccgccagt gatgacgcca caacacccac gaagaccgag
      541 acctggttgg ccaccgctac ccgctccgga tcgacgttga cgatctcgga gaagagacgc
      601 cgggaaccag ccaccgcctc gtcgtactcg gccatggtgg cgatgcctcg tcgccaccta
      661 tcgagtgctt cggtcatgac cgcgatcacc gagctggcgg ccaacccgta ggtaggagtg
      721 tccaggtaga cggtgtccgg gctgaactgg ggtcggcgga ccgggaggct catgtcaggc
      781 cgcgcctggt gcgtcggggt tggacgggat cggtcacccg gggagcgtaa gacctcgtcg
      841 cttgtaccgt tgccgtcatg cggttcctgg ccttcttcgc cctcatcgcc ctcgccgagg
      901 tggcgacctt cttctgggtg ggttcgaaga tcggtctgct ctgggcactc gggctggcga
      961 tcacaaccgc tctgctcggg gctttcctgg ttcgtcgcgc cgggctctcc gtcctcggcc
     1021 gaatccggga aagaatggat caaggacaac tccccgggcg cgagctcagt gacggtgccg
     1081 ccatcctcgt cgccggcgct ttcctgatct caccagggtt catcaccgat gccctggggt
     1141 tcctgcttct gatcccggcg gtgcgagcgc tgattcaccg tgtgttgtgg atacggctcg
     1201 ccgggcggat gagggtcttc accgtgaacc gccagacgat gtccggggtg tgggccgacc
     1261 ggaccgggtc atctggaggt cggcgagacg atgtgatcga tatcgacgac gtggagctat
     1321 tcccactcgc tgatccccgc gacgagcggg caaattcgcc actcccgtca ggggagcctg
     1381 ccgaaccagg cgagtgaccc gacaccggcc agcgccagga tcaccagcgc ggccccgacg
     1441 aaccagtccc ccacctcgac tggctcggtc tcgacctgga ccgagcgacc gagatccgcg
     1501 tacacctcgc tcagctgctc ggcagttgcc gcggtgagtg gccgaccgtc ggtctgacga
     1561 gctagatctt cgagtgcctt ggcattgacc gggaccggaa tcacctcgcc gtaggggtcg
     1621 acgatggtcc ccgaatcggt gccgaaggcg atggtgtgca ccgggatccc cgccccacgt
     1681 gccgcagacg cggcctcgtc gttggttcgg cccatggtgg tatcgccgtc ggagagcacc
     1741 acgatggtcc ccggcgcctc gccagtttcc tcgccggccc catcgacctg cgctgcggaa
     1801 gactcgatgg cgtcgatccc caggaacacc gcctcgccta tcgcggtgcc ctcccccagc
     1861 ccgttccgat cgatggcgct gtccacgacc cggcgcaccg cctcgaggtt ggtggtcggg
     1921 gcaatgagca gacgtgcctc gccgtcgaat ccgaccagcc cgacggccac accttcgggc
     1981 acggtgtcga ggaacgctcc ggcggactcg cgggcagcgt cgacccggga cggagtgacg
     2041 tcgtcggcct ccatcgagat cgaggtgtcg atggccagca ccacgagctg gatcgactcc
     2101 tgggtctctc ccgccaccac ggggcgagcg aaggcaacgg tggccacgac caccgccacc
     2161 agt
//
LOCUS       DATITW010000733         1635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_355992, whole genome shotgun sequence.
ACCESSION   DATITW010000733 DATITW010000000
VERSION     DATITW010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355992"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..571
                     /locus_tag="VLS86_10205"
     CDS             335..571
                     /locus_tag="VLS86_10205"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HSO50907.1"
                     /translation="MKRESATSHVAAAMGDGWIQGGSFLGSILSGTLLGYLADMWLGT
                     EPWLVVTGIVVGSYSGFLNVWRQSKRAEEAGRES"
     gene            561..974
                     /locus_tag="VLS86_10210"
     CDS             561..974
                     /locus_tag="VLS86_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50908.1"
                     /translation="MSLEHGPAIEADLARATVARAIYVGPALILLFGLLRGWEGAWSA
                     ALGVVVVIANFLLAGAILSISARISLQAYHAAALIGFFLRLVLFIGAVYLVASVVEVD
                     RVAFGISAVVAYFTLLTWEAVAMTKDREREPSWRR"
     gene            971..>1635
                     /locus_tag="VLS86_10215"
     CDS             971..>1635
                     /locus_tag="VLS86_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HSO50909.1"
                     /translation="MILAADCDVVNEVICAPANVTELFEFPTIFTIGNFEVTRTEILM
                     FLAAFIVLTLFYFGLRQQRLVPSKFGVAMEGMVGFVRDGIGRDVIGPESTKYVPYLLA
                     MFSFLLVGNLFSVTPLINFPITSRMAIPAFLSLVTYWIFVIVGFSKNHFRYLIDTAWP
                     KAVPIGLRWLVGLIELVSIFILRPITLAVRLFANMVAGHLMLTLLLGSGVIFVAAIPE
                     IGV"
BASE COUNT          253 a          512 c          511 g          359 t
ORIGIN      
        1 ggggatgaag gagccggaag cggccgaggt ggcgtcgctg atcgcccggg cgttgcaaaa
       61 tcgtgccgat ccctcggtgc tttcggaggt ggcggggcgg gtctccgagt tggcggccag
      121 tttctcgccc tatccccacg aattctctgg gcatgtttga agtatctccc ccatcctcgc
      181 tggagggtcg gcaggggttg tccccctgcg gagcgggggg accgacgcca gcgagcgcag
      241 cgagtgcgtc gaggggggcc aagtcgaccc gcaataagat ggattttgag tacttgatgc
      301 tgggactaac ctttgtgaaa gaatgcacaa gcccgtgaag cgtgaatcgg ccacgagcca
      361 tgttgccgca gccatgggcg acggttggat ccagggtggt tcgttcctcg gctcgatcct
      421 ctccgggacc ctcctggggt atctggccga catgtggctg ggcaccgagc cctggctggt
      481 cgtcaccggg atcgtggtcg gctcgtactc gggattcctg aacgtgtggc gtcaatcaaa
      541 acgagccgag gaggccggac gtgagtcttg agcacggacc ggccatcgaa gccgatctgg
      601 cacgagccac agtcgcccgc gccatctacg tcggccctgc tctgatcctg ctcttcggcc
      661 tgcttcgggg ctgggagggt gcttggagcg ccgcgctcgg agtggtggtg gtgatcgcca
      721 acttcctcct cgccggagcc atcctctcga tttcggcaag gatcagcctt caggcttatc
      781 atgccgccgc tctgatcggc ttcttcctac gtctggtcct gttcatcggc gcggtctact
      841 tggttgcgag cgtcgtcgag gtggaccggg tggcgttcgg gatctcggca gtggtcgcct
      901 acttcacact gctgacctgg gaagccgtag cgatgaccaa ggacagagaa agggaaccca
      961 gttggcgcag atgatcctcg ccgccgattg tgacgtagtc aacgaggtga tctgcgcccc
     1021 ggcgaacgtg accgagctgt tcgagttccc caccatcttc accatcggca acttcgaggt
     1081 gacccgcacc gagatcctca tgttcctcgc cgccttcatc gtgctgaccc tcttctactt
     1141 cgggctccgc cagcagcggc tggtcccttc caaattcggc gtagcgatgg agggcatggt
     1201 cggtttcgtg cgcgacggga tcggccgtga cgtcatcggg ccggagtcga ccaagtacgt
     1261 cccctacctg ctggcgatgt tctcctttct cctggtcggc aacctgttct cggtcacccc
     1321 gctgatcaac ttcccgatca cctcccggat ggcgattcct gccttcctct ccctggtcac
     1381 ctactggatc ttcgtgatcg tcggcttctc caagaaccac ttcaggtacc tgatcgacac
     1441 cgcatggccg aaggcggttc ccatcggtct caggtggctg gtcggtctca tcgagctggt
     1501 ctcgatcttc atcttgcgac cgatcacgct tgcggtgcgg ctcttcgcca acatggtggc
     1561 cggtcacctg atgctgacgc tgctcctcgg atccggcgtc atcttcgtag ccgccatccc
     1621 cgagatcggg gtcaa
//
LOCUS       DATITW010000734         1661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_516299, whole genome shotgun sequence.
ACCESSION   DATITW010000734 DATITW010000000
VERSION     DATITW010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516299"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..917
                     /locus_tag="VLS86_10220"
     CDS             216..917
                     /locus_tag="VLS86_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSO50910.1"
                     /translation="MDIILIPGLWLDGSSWEKVTPLLEEAGHRTHALTLPGMESMHAD
                     RSAVTRRDHVDAVVAALDSIDPADGKVVLVGHSAGGAIAHAAVDARPDRVARAIYVGG
                     FPLADGEGDPGEFPAENGEVPLPDWSDFEEEDLADLDDEAKADFRERAIPSPAGVTRD
                     LQRLVDERRFDVPITVICTEFSSEMLRSWIDKGLAPVRELSVILKVEYVDLPTGHWPQ
                     FSRPEELAGAILRAL"
     gene            968..1597
                     /locus_tag="VLS86_10225"
     CDS             968..1597
                     /locus_tag="VLS86_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HSO50911.1"
                     /translation="MTRTADLDDPSSPNGKEVLTPPSRAAWRRWLASNPDRHEGLWVV
                     YRKKSSSLDGPMYDDLVEEALCFGWIDSQARRVDDDRVIQWFSPRRKGGLWSTINKAR
                     IERLVDQGLMTEAGQAAIDEAKADGSFTQADEVDALIVPADLKAALDGAPGAKTAYQA
                     LANSAKREHLWWIHTAKRAETRASRIEETIRRLSSESPPSGPNGDSPDR"
BASE COUNT          298 a          556 c          544 g          263 t
ORIGIN      
        1 aggatggtgc tccggatctg gtgggccatg gcccgaagct cctgcggaga cgacaccacg
       61 agcacgtcct cgaggttgta atcgggggtg ggggcattgt tcgacaaatt tggatcgttc
      121 cgctaccttg cgaatgatcg agaagtttgg atcataaccg gtcccatcgt cacgggctgg
      181 aagcgcaacc cggcagccaa gaagaacggc acgcaatgga catcattctg atccccggtc
      241 tctggctcga cggctcatcg tgggagaagg tcaccccgct cctggaggag gcgggccatc
      301 gcacccatgc cctcaccctg cccggcatgg agtcgatgca tgccgaccgc tccgcggtca
      361 cccgacgtga tcacgtcgat gcggtggtcg ccgccctcga ctcgatcgac ccggccgacg
      421 gcaaggtggt tctggtcggg cactccgccg ggggcgccat cgcccacgcc gcggtcgacg
      481 ccagacctga ccgtgtcgcc agagcgatct acgtcggtgg cttcccgttg gctgatggcg
      541 aaggcgatcc cggcgagttc ccggccgaga acggggaggt gcccctgccc gactggtcgg
      601 acttcgagga ggaggacctg gccgatctcg acgacgaggc gaaggctgac ttccgggagc
      661 gggcgatccc atcgcccgcc ggcgtcaccc gcgatctgca gcgactcgtc gatgagcgcc
      721 gcttcgacgt tccgatcacc gtgatctgca ccgagttcag cagcgagatg ctccggagct
      781 ggatcgacaa gggcctggct ccggtgcgcg agctctccgt gatcttgaag gtggagtatg
      841 tcgacctgcc caccggccac tggccgcagt tctcacggcc cgaagaattg gcgggggcca
      901 tcctccgggc tctctgaccg gcaccgaccc gcgccggcat cgcggaccgc gcattaggtt
      961 gggcgagatg acacggaccg ccgacctcga cgatccgtct tccccaaacg gcaaggaagt
     1021 cctgaccccg ccgagtcgtg ccgcctggcg tcggtggctc gcttccaacc ccgaccggca
     1081 cgagggactg tgggtggtct accgcaagaa gtcgagcagc ctcgacggcc cgatgtacga
     1141 cgacctggtc gaagaggcac tgtgctttgg gtggatcgac agtcaggcgc ggcgagtaga
     1201 cgacgaccgc gtgatccagt ggttctcccc ccgccgcaag ggcggactgt ggtcgacgat
     1261 caacaaggcc cggatcgagc gtctggtgga ccagggcctg atgaccgagg ccggtcaggc
     1321 ggcgatcgac gaggcgaagg ccgacggctc attcacccag gccgacgagg tcgacgccct
     1381 gatcgtccct gctgatctga aagccgctct ggatggcgct cccggggcca agaccgcata
     1441 tcaagccctg gccaactcgg cgaagcggga acatctgtgg tggatccaca ccgccaaacg
     1501 tgccgagacc cgtgccagcc gaatcgagga gaccatccgc aggctctcct ctgagtcacc
     1561 accctctggc ccgaacggtg acagccccga ccgatgagct cccagtcacc actcctgccc
     1621 ggaggtggtc agcccagcaa caccacgatc gtctgggcga g
//
LOCUS       DATITW010000735         1814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_676340, whole genome shotgun sequence.
ACCESSION   DATITW010000735 DATITW010000000
VERSION     DATITW010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676340"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1814
                     /locus_tag="VLS86_10230"
     CDS             <1..>1814
                     /locus_tag="VLS86_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSO50912.1"
                     /translation="ALDRARDLVKEESELAAALAPTWGEQAPEAGAHPALFLDLEHSL
                     AGRRYLGVPSTPTGPGDAVLEIRALDAVPGDPESVAAGLGRLLARGIETVVAMDGDPA
                     ADRVAGALGEHGLLLDRLQSLTHRGSAILPVGIHKGFVAPGLGVAVLGEQEIAGRRRA
                     HRKIGRARSPGPQQYRDLTEGDYVVHHQHGIGRFEGLVSRTMAGIERDYLVIGYAGED
                     RLYVPVDQLAAVRRYTGGEVPRVSRMGGRDWSEQRSRVRRAVAAVAQEVVELHRMRAA
                     ATGHAYDPDTPWQLEMEGAFPFEETHDQMQAIVDVKADMESGQPMDRLVFGDVGYGKT
                     EVAIRAAFKAVQAGKQVAILVPTTLLAQQHHQVFTERFESYPVRVEALSRFLSPRQQK
                     TVIAGLASGEVDVVIGTHRLLSDDIRFKALGLLVIDEEQRFGVTAKDAMKRLKVGVDV
                     LTLTATPIPRTLEMALTGIREVSHIRTPPEDRHPILTYVGAYDDQAVSAAIRRELLRE
                     GQVFYVHNRVQSIDRAVARLQELVPDARYVVAHGQMSEGQLEQVMIDFWTGEYDVMVA
                     TTIIESGLDLPKVNTLIVERADLLGLAQLYQLRGRVGRS"
BASE COUNT          297 a          594 c          639 g          284 t
ORIGIN      
        1 gccctcgacc gggctcgaga cctggtgaag gaggagtcgg agctggccgc cgcactggcg
       61 ccgacctggg gagagcaggc gcccgaggcc ggggcccatc ccgccctctt cctcgatctc
      121 gagcacagtc tcgccggacg ccgctatctc ggtgtgccat ccaccccgac cgggcccggg
      181 gacgcggtcc tggagatcag ggccctggac gcggttcccg gcgacccgga gtcggtggct
      241 gcagggctcg gtcgactgct ggcacgggga atcgaaacgg tggtcgcgat ggatggcgac
      301 ccggcggccg atcgggtggc tggcgcgctc ggcgagcatg gtctccttct cgaccggctg
      361 cagtcgctca cccaccgggg gtcggcgatc ctcccggtcg ggatccacaa ggggttcgtg
      421 gccccaggcc tgggggtggc ggtcctcggc gagcaggaga tcgccggccg gcgtcgcgcc
      481 catcgcaaga tcggccgggc ccgaagcccc ggcccgcagc aataccgcga cctgaccgaa
      541 ggcgactacg tggtccacca ccaacatggc atcggccgtt tcgaggggct ggtatcgcgg
      601 acgatggccg ggatcgaacg cgactatctc gtcatcggct atgccgggga ggaccgtctc
      661 tacgttccgg tcgatcagct ggcagccgtg cgtcgttaca ccggcggcga ggtgccccgg
      721 gtgtcgcgca tggggggcag ggactggtcc gagcagagga gcagggtgcg ccgggccgtg
      781 gcggcggtcg cccaggaggt ggtcgagctg catcggatgc gggctgcggc gacggggcac
      841 gcctatgacc ctgacacccc gtggcagctg gagatggagg gggcgttccc cttcgaggag
      901 acccacgacc agatgcaggc catcgtcgac gtcaaggccg acatggaatc gggtcagccc
      961 atggaccgcc tggtgttcgg cgacgtcggc tacgggaaga ccgaggttgc gatcagggct
     1021 gcgttcaaag ccgtgcaggc cgggaagcag gtggcgatcc tggtcccgac cacgttgctc
     1081 gcccagcagc accaccaggt cttcaccgag cgattcgaga gttacccggt ccgggtcgag
     1141 gcgctgtccc ggttcctctc accgcgacaa cagaagacag tgatcgccgg tctggccagc
     1201 ggggaggtcg acgtcgtgat cggcacccat cgtctgctat ccgatgacat acggttcaag
     1261 gcgctcggtc ttctcgtgat cgacgaggag caacgattcg gggtgaccgc caaagacgcc
     1321 atgaaacggc tcaaagtcgg agtcgacgtc ctgactctca ccgcgacgcc gatcccgcgg
     1381 acactggaga tggctctcac cgggatccgt gaggtctccc acatcaggac tccgcccgag
     1441 gaccgccatc ccatcctcac ctatgtcggg gcctacgacg accaggcggt ctccgcggcg
     1501 atccgccgtg agctcctgcg tgaaggccag gtcttctacg tccacaaccg ggtgcaatcg
     1561 atcgatcggg cggtggcccg tctccaggag ctcgtccccg atgcccgcta tgtggtggct
     1621 cacggccaga tgtccgaggg ccagctggaa caggtcatga tcgacttctg gaccggcgaa
     1681 tacgacgtga tggtggcgac cacgatcatc gaatcgggtc tcgacctgcc caaggtgaac
     1741 accctcatcg tggagcgggc ggatctgctc ggcctggcac agctctacca gctccggggg
     1801 agggtcgggc gatc
//
LOCUS       DATITW010000736         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_637178, whole genome shotgun sequence.
ACCESSION   DATITW010000736 DATITW010000000
VERSION     DATITW010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637178"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1241
                     /locus_tag="VLS86_10235"
     CDS             90..1241
                     /locus_tag="VLS86_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSO50913.1"
                     /translation="MTTPLIDGDKAPVTATVPVEEGATDHSLLVWFLRILSALVVPAV
                     LVLFFFTLEFLRGDVNKVIQVLVAVLVGVLGIWALYWGMDRLVSALPSRAAAGVRPYA
                     FAGPAMVLLGFYLVYPAVNTFILSFQNDAGDAFVGIANYQRIFTESTYLVAIRNSIAW
                     VIIVPLTAVIIGLTFATLADKLPARSESAAKSLIFLPLAISFVGASVVWAFIYRFRPE
                     GFGDQIGILNAIMVGVGKEPVDWLALPVWNSLFLMVILVWLQTGFAMVILSSAIKAVP
                     EEITEAARIDGANEWHVFRRIVLPSISSTVVVVWTTILITTWKVFDIVYVMTGGREGT
                     SVVAQQMVTEFFTFNNDGMGAALAVILILEVVPILVINIRRVREQEATR"
     gene            1238..2143
                     /locus_tag="VLS86_10240"
     CDS             1238..2143
                     /locus_tag="VLS86_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSO50914.1"
                     /translation="MTSTTDTVQAPQAPRPSRPTVKAKASRFPVYLAVGLITMLWTIP
                     SLGLLINSFRAPQSIRASGWWTVIGEVFDSATWTIQNYQEVLATGFSNAFANTVAVTI
                     PSVVIPITVAAFAAYAFAWMDFPGRNLIFIAVVGLLVVPLQMALIPVLRIYTRFDLNG
                     TFPAVWLAHAGFGLPLAIYLLRNYMGSISSSLIESAKIDGADHFTIFTRLVVPLSVPV
                     LASFAIFQFLWVWNDFLVAIVFLGYDPAIRVLTVELSDLAGRGEGSLSLLPAAAFVTM
                     ILPLVVFFALQRYFVRGLTAGSVKG"
BASE COUNT          355 a          713 c          624 g          508 t
ORIGIN      
        1 tggccgcggc ggccacccca ttcttttgtc cggcttcacc cttaccggtt gggtagggtc
       61 gaccgaaccg gtgttgagaa aggagtgaag tgaccacacc gctcatcgac ggagacaaag
      121 ctccggtcac ggcaaccgta cctgtcgaag agggcgcgac cgaccactcg cttctggtct
      181 ggttcctccg aatcctcagc gcgctcgtgg ttccagcggt cctggtcctg ttcttcttca
      241 ctctcgagtt cctccgcggc gacgtcaaca aggtcatcca ggttttggtg gccgtgctcg
      301 tcggtgtgct cgggatctgg gccctgtatt ggggaatgga ccggttggtg agcgcgcttc
      361 catccagggc tgcagccggt gtccgcccct acgcttttgc cggtccggcg atggtcttgc
      421 tcggcttcta tctcgtctac cccgcggtca acaccttcat cctcagcttc cagaacgacg
      481 ccggtgacgc cttcgtcggg atcgccaact accagcgcat attcaccgag agcacatatc
      541 tggtcgccat ccgaaacagc attgcctggg tcatcatcgt ccctctcacc gcagtgatca
      601 tcgggttgac cttcgccacg ctcgccgaca agctccccgc ccgctcggag tcggccgcaa
      661 agtcattgat cttcctcccc ttggccatct cgttcgtcgg cgcctcggtg gtttgggcgt
      721 tcatctaccg gttccggccc gagggattcg gcgatcagat cgggatcctc aacgcgatca
      781 tggtcggcgt tgggaaggag ccggtggact ggctagctct tccggtctgg aacagcctgt
      841 tcctgatggt gattctcgtc tggctccaga ccggcttcgc aatggtcata ctttcatcgg
      901 ccatcaaggc cgtgccggaa gagataaccg aggccgcccg aatcgacggc gccaacgaat
      961 ggcacgtgtt caggaggatc gtcctcccct cgatctcgtc gacggtggtc gtggtctgga
     1021 ccacgatcct catcacaacc tggaaggtct tcgacatcgt ctatgtgatg accggtggcc
     1081 gtgaaggcac ctcggtcgtc gcacagcaga tggtgaccga gttcttcacc ttcaacaacg
     1141 acggcatggg cgccgcgcta gcggtgatcc tgatcctcga agtggtcccg atcctggtaa
     1201 tcaacatccg aagggtccga gaacaggagg caaccaggtg acctccacca ccgacaccgt
     1261 gcaggcaccg caagctcctc gcccgtcccg gcctaccgtg aaggccaagg cgtctcgatt
     1321 tccggtctat ctggcggtcg gtctgatcac gatgctctgg acaatcccat cgttgggctt
     1381 gctgatcaac tccttccggg cgccgcaatc gatccgagcc agcggctggt ggactgttat
     1441 tggcgaggtc ttcgactcgg cgacctggac catccagaac taccaagagg tcctggccac
     1501 aggcttctcc aatgccttcg caaataccgt ggccgtgacg atcccatcag tggtcatccc
     1561 gatcaccgtc gccgcctttg ccgcgtatgc attcgcatgg atggactttc cgggtcgcaa
     1621 cctgatcttc atcgccgtgg tgggtttgct ggtggtgcct ttgcagatgg ccctgatccc
     1681 cgtacttcgc atttacacca gattcgatct gaacggcacg tttcccgccg tctggctcgc
     1741 ccacgccggg ttcgggctgc cactcgccat ctatctgcta cgcaactaca tgggttcgat
     1801 ctcgtcgtcg ctcatcgagt cggccaagat agatggcgcc gaccatttca ccatcttcac
     1861 cagactcgtc gtgcctcttt cggtcccggt ccttgcgtcc ttcgccatct ttcagttcct
     1921 ctgggtgtgg aacgacttct tggtggcgat cgtgttcctc ggctacgacc ctgcgatcag
     1981 ggtgctcacc gtggagttgt ccgacctcgc cggtcgtgga gaaggctctc tgagtctgct
     2041 tcctgccgcc gcgttcgtca ccatgattct tccgctcgtc gtcttcttcg cattgcagcg
     2101 gtacttcgta agaggcctaa cggccggttc cgtgaagggc taggtaatcc tggcccggtc
     2161 ggagacgccg gagtctgccc gctggcggaa ctggcttgcg
//
LOCUS       DATITW010000737         1743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_717396, whole genome shotgun sequence.
ACCESSION   DATITW010000737 DATITW010000000
VERSION     DATITW010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717396"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VLS86_10245"
     CDS             complement(<1..215)
                     /locus_tag="VLS86_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HSO50915.1"
                     /translation="MKDLLEGLLQGSDLTVEEAETLLRLLTGGEMDPASAGAVLIALR
                     AKGETPEEVLGFARAMRELATDPEIDV"
     gene            complement(212..796)
                     /locus_tag="VLS86_10250"
     CDS             complement(212..796)
                     /locus_tag="VLS86_10250"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HSO50916.1"
                     /translation="MSKVLLIDNYDSFTYNVVQSLRILGAEVVVRTNDEISLDEAREM
                     EATHLVISPGPGRPEAAGESMKLIAGMLEEVPILGVCLGHQALAAALGGTVDHAEELR
                     HGKASPVYHDSATIYAGLPNPFDAGRYHSLAVMEEDLPDDLVVTAFTSDGVIMGIRHR
                     SLPVEGVQFHPESLLTPHGDLLLRNFLDLGGSKR"
     gene            complement(793..>1743)
                     /locus_tag="VLS86_10255"
     CDS             complement(793..>1743)
                     /locus_tag="VLS86_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HSO50917.1"
                     /translation="SRRAALLHDGSDAERLALRQEVMALLSQGLPSRAVTGHFSAPVA
                     SMSKEQYEAVVVRAQHHIAEGDVYQIVPSVRFSGESDLDPFTAYRGLRLLNPSPYMYF
                     CDLGSTKVIGSSPEALVRLRGTTAELRPIAGTRPRHGDDDRDRRAEEELIGDAKEAAE
                     HVMLVDLARNDLGRVAVPGSIKVDPYRAIERYSHVIHLVSGVSGILGPGVDAFDLFAA
                     AFPAGTVVGAPKIRALELLEEMEPVDRSLYAGTVGYFGHGGTMDQAIAIRTIVMSEGE
                     YSFQAGAGIVADSVPAAEWEEVLSKSGAMRGALEAAAGGL"
BASE COUNT          291 a          604 c          571 g          277 t
ORIGIN      
        1 ctgacatcga tctcggggtc ggtggccagc tcccgcatgg cccgggcgaa cccaaggact
       61 tcctccggcg tctccccctt ggcccggaga gcgatcagca ccgccccggc cgatgccgga
      121 tccatctccc cacccgtcag cagacgcaac aatgtctcgg cttcttcgac cgtgaggtct
      181 gatccctgca gcagaccctc cagcaagtcc ttcatcgctt ggagcctccg aggtcgagga
      241 agttgcggag aagcagatcg ccatgtgggg tgagcaggct ctcggggtgg aactggacgc
      301 cttcgaccgg aagggagcga tggcggatcc ccatgatcac accgtccgag gtgaaggcgg
      361 tcaccaccag gtcgtcgggg agatcctcct ccatcacggc cagcgagtgg tagcgcccgg
      421 cgtcgaacgg gttgggcagc ccggcataga tggtggccga gtcgtggtag acgggtgacg
      481 ccttgccgtg acgaagctcc tcggcgtgat ccacggtgcc gccgagggca gcggccaggg
      541 cctggtgacc gagacagaca ccgaggatcg gcacctcctc gagcatcccg gcgatcagct
      601 tcatcgactc cccggcggct tcgggccgcc ccgggcctgg agagatgacg agatgggtgg
      661 cctccatctc ccgcgcctcg tcgaggctga tctcgtcgtt ggtccgaacc actacctcgg
      721 cgccgaggat gcggagcgac tgaacgacgt tgtaggtgaa cgagtcgtag ttgtcgatga
      781 ggaggacctt gctcatagcc cacccgctgc cgcttccagg gccccgcgca tcgcccccga
      841 cttggacagg acttcctccc attccgccgc cgggacgctg tcggcgacga tgccggcccc
      901 ggcctgaaac gagtactcgc cctcggacat cacgatggtc cggatcgcga tcgcctgatc
      961 catggttcct ccatgaccga agtatccgac ggtgccggcg taaagggagc ggtcgacggg
     1021 ctccatctcc tccaggagct cgagcgcccg gatcttgggg gcgccgacca cggtgcccgc
     1081 gggaaacgca gccgcaaaca ggtcgaaggc gtcgaccccc gggcccagga tcccgctcac
     1141 gccgctcacg agatggatca cgtggctgta cctctcgatc gcccggtagg ggtccacctt
     1201 gatcgagccc ggcaccgcca cccggcccag gtcgttcctg gccaagtcga ccagcatcac
     1261 gtgctcggcg gcctccttgg cgtcaccgat cagctcttcc tcggcgcgac ggtcacgatc
     1321 gtcgtcaccg tggcgggggc gggtccccgc aatcgggcgc aactccgcgg tggtcccccg
     1381 caagcggacc agggcctcgg gcgaggagcc gatcaccttg gtcgacccca ggtcgcagaa
     1441 gtacatgtag ggagacgggt tgagcaggcg cagcccgcga tacgccgtga acgggtcgag
     1501 atcgctctcc ccggagaacc tgaccgaggg cacgatctga tacacgtccc cttcggcgat
     1561 gtggtgctgg gctcgcacca ccaccgcctc gtactgctct ttgctcatcg acgccaccgg
     1621 cgcgctgaag tggccggtga ctgcccgcga ggggaggcct tgggagagaa gagccatcac
     1681 ctcctgtcgc aatgcgagac gctcggcgtc cgacccgtcg tggagcagag ccgcccgccg
     1741 gct
//
LOCUS       DATITW010000738         1643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_436834, whole genome shotgun sequence.
ACCESSION   DATITW010000738 DATITW010000000
VERSION     DATITW010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436834"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..924
                     /locus_tag="VLS86_10260"
     CDS             118..924
                     /locus_tag="VLS86_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HSO50918.1"
                     /translation="MISTILINGEPSRDGVPVTDSSVLRGDGCFEVLKAYNGVPFTLD
                     AHLDRLERSAAALEITLPARSELARWVEAVSSECPDCAIRVVVTRGSALPDEPGESMV
                     IVYAHPWAGSRGPARLLPVVAPWHAAGVDWDLAGAKVLSYAPNLSASRRASHAGFDDA
                     LLITVDEVMLEGPTFCVAWVVDGVLETPSLDLGILDSITRRVVLEDANRLGVPVVEGA
                     WALDRLQQASEVMAMSTIREIQPVSMVGEMAFGEGPVTSDLARAFAQRLS"
     gene            complement(909..1397)
                     /locus_tag="VLS86_10265"
     CDS             complement(909..1397)
                     /locus_tag="VLS86_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50919.1"
                     /translation="MGLDFCPDQVADPAFVRTVVRHLTGTEPPLIEGRSQEMPQVLEP
                     LGDEQRVIGELMGCLTPEPCLAFGVPESELARIAAWALDHIQSGAAPDAEFVSDLGQL
                     DTLPSAAEAEQQLRAKGLIGACWPPLDHDQLATREQVLTNLVRWIAGPAIDCVGTSQL
                     RR"
     gene            complement(1398..>1643)
                     /locus_tag="VLS86_10270"
     CDS             complement(1398..>1643)
                     /locus_tag="VLS86_10270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSO50920.1"
                     /translation="GEVIGWVGDSGNAEDSVDHLHFELRAPNGEAVDPAPSLRKARLQ
                     AELPEDEPIWPYLDDEARGMHWIAATLLTEGMFVPGD"
BASE COUNT          262 a          530 c          556 g          295 t
ORIGIN      
        1 ggtcgaccgc accgagtaca agcggagaca ggcagccccg ggggtgaaga tcaccaccaa
       61 ggcttttggc aaagatcgac gtcttcccat caccaacgcc taccagccac ctgtgccgtg
      121 atctcaacga tcctgatcaa cggcgagcct tcccgagacg gtgttccggt caccgattcc
      181 tcggtcctgc gcggcgacgg gtgcttcgag gtgctcaagg cctacaacgg ggtcccgttc
      241 accctcgacg cccacctgga ccggctggaa cggagcgccg cagcactgga gatcacgctg
      301 cccgctcggt ctgaactggc gcggtgggta gaggcggtgt cgagcgaatg ccccgattgt
      361 gcgatccggg tggtggtgac caggggctcg gctctccccg acgagccggg ggagtccatg
      421 gtgatcgtct acgcccatcc ctgggccggc agccgaggac cggcccggct tcttcctgtg
      481 gtcgccccgt ggcacgcggc cggcgtcgat tgggatctcg ccggcgccaa ggtcctctcc
      541 tatgccccga atctgtcggc cagcaggcgg gccagccatg cgggctttga cgatgcccta
      601 ctgatcacgg tcgacgaggt gatgctcgag ggcccgacct tctgtgtggc ttgggtggtg
      661 gacggggtgc tggagacccc gtctcttgac ctggggatcc tggactcgat cacccgccgt
      721 gtggtcctcg aggacgcaaa tcgcttgggc gtgccggtcg tcgagggggc atgggccctc
      781 gatcggttgc agcaggcgtc ggaggtgatg gcgatgtcga cgattcgcga gatccagcca
      841 gtgtcgatgg tcggcgagat ggcctttggc gaagggccgg tcaccagtga tctggctcgg
      901 gccttcgctc agcgtctgag ctgactggta ccgacacaat cgatcgcggg gccagcgatc
      961 catcgcacga gattggtcag cacctgttcc cgggtcgcca gctggtcgtg gtcgagcggt
     1021 ggccagcagg ccccgatcag ccccttggcg cgcagttgct gctcggcctc agctgctgag
     1081 gggagggtgt ccaattgccc caagtcgctc acgaactcgg catccggggc cgcaccgctt
     1141 tggatgtggt ccagggccca ggctgcgatt ctcgccagtt ccgattccgg gacaccgaat
     1201 gcgagacatg gctccggggt cagacacccc atcagctcac cgatcacacg ctgctcgtcg
     1261 cctaggggct ccagcacctg tggcatctct tgagagcgcc cttcgatgag agggggctcg
     1321 gtgccggtga gatgacggac gacggtgcgg acgaacgcag ggtccgctac ctgatccggg
     1381 cagaagtcga ggcccaccta atcaccgggc acgaacatcc cttcggtgag cagtgtcgcg
     1441 gcgatccagt gcatgccccg tgcctcgtcg tcgaggtaag gccagatggg ctcgtcttcg
     1501 ggaagttccg cctggagcct cgccttgcga agactggggg ccgggtcgac cgcctcgccg
     1561 ttaggggcgc gaagctcgaa atggagatgg tcgacactgt cctcggcatt gcccgagtcc
     1621 cccacccaac cgatgacctc gcc
//
LOCUS       DATITW010000739         1967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_757015, whole genome shotgun sequence.
ACCESSION   DATITW010000739 DATITW010000000
VERSION     DATITW010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757015"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="VLS86_10275"
     CDS             <1..1296
                     /locus_tag="VLS86_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50921.1"
                     /translation="RPVVVFLSNLDDLSWLTRETLVALEHELRATILWNIVPDSIEDV
                     GGYPMAYFSDRRTPDLQFDHVVYSIPAPSETRLPVSDASDDRDVFFVGDPGQTGVIHP
                     NEAWSHLRSRSFPELPEPPVLIRKTGLGPGEADRLRLENYNATITHFDRAHNELWVLR
                     IEPDSRDVSHLPGQYATLGLGYWEPRVDDAGEDLSPERARAMIRRSYSISSRIFDSRG
                     YLVDPALENAIEFYIVHVTPDEGKVPALTPRLAAKAEGDRIYIGPKIVGRYTLAPVDD
                     PGLDVVFMATGTGEAPHNAMIAELLRRGHFGARVSLVTVRYAEDLAYASTYESLQSRF
                     PNVRYLPLVTRDPVGPKRYIQDLIRSGEQPDLLPHHLDPRRTQVDLCGNPAMIGLPVW
                     ESDQPEFPEPQGVCQLLLDRGFTLDRRGVIGNVHYEEYW"
     gene            complement(1307..1519)
                     /locus_tag="VLS86_10280"
     CDS             complement(1307..1519)
                     /locus_tag="VLS86_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50922.1"
                     /translation="MSDGVTRMEIADAVQPAFERGGADREEILAMASSKQTRPEVLAV
                     LSGLTKRRYSRLNQLWEELVDIPVKF"
     gene            1636..>1967
                     /locus_tag="VLS86_10285"
     CDS             1636..>1967
                     /locus_tag="VLS86_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019356193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSO50923.1"
                     /translation="MIDEHHQIGAVAERLDLSLRTIRYYEEIGLVSPSGRTEGGFRLY
                     TESDIERLRLVKVLKPIGMSLEAMSELLECADRLGSGHGEDLEEVGKRLEAVVEMAIG
                     RCDELEQR"
BASE COUNT          355 a          631 c          620 g          361 t
ORIGIN      
        1 cggccggtcg tggtgttcct ctccaatctc gatgaccttt cctggctgac cagggagacc
       61 ctggtcgccc tggagcacga gctccgggcg accattctct ggaacatcgt gccggattcg
      121 atcgaagacg tcggcgggta cccgatggcg tacttctccg atcgcagaac tcccgatctc
      181 cagttcgatc acgtggtgta ttcgatcccg gccccgagcg agaccaggct gccggtctcc
      241 gacgcgagcg acgaccgcga cgtgttcttc gtcggcgatc cagggcagac gggtgtgatc
      301 catcccaacg aggcttggtc ccatctccgg agccggagct ttcccgagct acccgagccg
      361 ccggtcctga tccgaaaaac cggcctcggc cctggcgagg cagaccgcct gcggctcgag
      421 aactacaacg cgaccatcac ccacttcgac cgggcgcaca acgagctgtg ggtgcttcgg
      481 atcgagcccg attcacgcga cgtgtcacac ctgccggggc agtacgccac gctaggtctg
      541 ggatattggg agcccagggt cgatgacgcg ggggaggatc tgtccccgga gcgggccagg
      601 gccatgatca ggaggtcgta ctcgatctcg agtcgtatct tcgattcccg cggctatctg
      661 gtcgatcctg cgctggagaa cgcgatcgag ttctacatcg tgcacgtcac gcccgatgag
      721 gggaaggtgc cggccctcac cccccggctt gcggccaagg ccgaagggga tcggatctac
      781 atcgggccca agatcgtcgg gcgatacacc ctggccccgg tggacgaccc cggtttggat
      841 gtggtgttca tggccaccgg caccggcgag gcgccacaca acgcgatgat cgccgagctg
      901 ctccgacggg ggcatttcgg ggccagggtg tcactggtga cggttcgcta cgccgaggac
      961 ctcgcctacg cgtcgaccta tgagagtctt cagtcccgct ttcccaacgt gcgctatcta
     1021 cccctcgtca cccgcgaccc ggtcggcccc aagcgataca tccaggactt gatccgcagc
     1081 ggtgagcagc ccgacctgct cccccatcac ctcgacccgc ggcgaaccca ggtcgaccta
     1141 tgtggcaatc cggcgatgat cgggttgccg gtctgggagt cggaccagcc ggagttcccc
     1201 gagccgcagg gggtttgcca gttgctcctc gaccgggggt tcaccctcga tcgacgcggc
     1261 gtgatcggca acgtccacta cgaggagtac tggtagaccg gtcgaatcag aacttgaccg
     1321 ggatgtcgac gagctcttcc cagagctggt tgagacggga gtagcgacgc ttggtcagac
     1381 cggagaggac cgcgagcacc tcggggcggg tctgtttgct ggaggccatg gcgaggattt
     1441 cctcacggtc ggccccacca cgctcgaagg cgggctgcac cgcgtctgcg atctccatcc
     1501 tggtcactcc gtcactcacc gattcgctcc atttcaactc ttcgtttacg ttagggtaga
     1561 gtgtacctca gtggtggagc gttcccaaaa cgcctctttt gcgcgacgcc cacaccaaac
     1621 catccaggga aagcaatgat cgacgaacat catcaaatag gagccgtggc cgagcgtctc
     1681 gatctctcgt tgcgcaccat ccgctactac gaggagatcg gtctggtcag cccctccggg
     1741 cgcaccgagg gtgggtttcg cctctatacc gaatcggaca tcgagcgact gcgtctggtc
     1801 aaggtgctca aaccgatcgg gatgtcgctc gaagccatga gcgagttgct ggaatgcgca
     1861 gatcgtctcg gaagcggaca cggagaggac ctcgaggagg tcgggaagcg gctcgaggcc
     1921 gtcgtcgaga tggccattgg ccgttgcgac gagctcgagc agagatt
//
LOCUS       DATITW010000740         2744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_196019, whole genome shotgun sequence.
ACCESSION   DATITW010000740 DATITW010000000
VERSION     DATITW010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196019"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VLS86_10290"
     CDS             complement(<1..213)
                     /locus_tag="VLS86_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50924.1"
                     /translation="MSDRTDSERDKGFRRPWETEDSEGDAEVFTLPDTERPAPSDVDL
                     TDWDAMTSDNGALEDYTAEDYAAATTE"
     gene            complement(210..761)
                     /gene="frr"
                     /locus_tag="VLS86_10295"
     CDS             complement(210..761)
                     /gene="frr"
                     /locus_tag="VLS86_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HSO50925.1"
                     /translation="MISDLLTEAEHKMEMAIDHVATEFSTVRTGRANPQLLQRIHVDY
                     YGSPTPLQQLASIAVPEPRLLVVNPFDRSTVSDIERAIQQSDLGLNPTNDGSVIRIAF
                     PPLTEERRKDLIKVVKHMAEEGRVAVRNIRRHLKTDMEALHGEISDDDIRRGEEDLQK
                     LTDRFVVKIDHLLAGKEEELLEV"
     gene            complement(776..1489)
                     /gene="pyrH"
                     /locus_tag="VLS86_10300"
     CDS             complement(776..1489)
                     /gene="pyrH"
                     /locus_tag="VLS86_10300"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HSO50926.1"
                     /translation="MTDRPRRVVLKLSGEAFADPSIGYGIDPATVVRVAGEVAAAHAT
                     GVQIAVVVGGGNIFRGVSQAAKGMDPASADYMGMLATVINALALRDALEKQGVPTRVQ
                     SAITMQELAEPYIRLRAIRHLEKGRAVIFAAGTGNPLITTDTTAALRAVEIGADVLLK
                     ASKVDGIYDADPATNSDANRMGDLSYMDFISRELRVMDTTAVTMCKEHGVPIVVFDMF
                     APGNILKAVNGERIGTVIH"
     gene            complement(1497..2108)
                     /gene="tsf"
                     /locus_tag="VLS86_10305"
     CDS             complement(1497..2108)
                     /gene="tsf"
                     /locus_tag="VLS86_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336547.1"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HSO50927.1"
                     /translation="MADFTAKDIQKLRVDAGVGMMDAKQALTDADGDFDKAFELLRER
                     GQASIAKRAGREANEGTIGTYIHIQNGRPVMGVMVELACETDFVAKSEEFRETADDIA
                     MHISWGNPSWVTREQADPDEVAKERDLLARQASAEGKPEGVVERIVEGRLSSWYTDRV
                     LYDQKFVNAEKFEGTVGDLVSQLAAKMGENISVRKFARVAVGE"
     gene            complement(2115..>2744)
                     /gene="rpsB"
                     /locus_tag="VLS86_10310"
     CDS             complement(2115..>2744)
                     /gene="rpsB"
                     /locus_tag="VLS86_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HSO50928.1"
                     /translation="GERNGIHIIDLRQTLEQINDATAYVKDLVAGGGTVLFVGTKKQA
                     QTAVAEHASRSGMPYVNFRWLGGMLTNFATIQKRIFYMRELRRLEESGEINSLPKKER
                     LKLRRELGKLEQNLGGVADLQRVPDAVFVIDVNVETTAVTEASRLGLPVIALVDSNCD
                     PDQVEYVIPCNDDAIRAADLIAGALADAALEGRELATAKTAKADDVEES"
BASE COUNT          424 a          900 c          858 g          562 t
ORIGIN      
        1 ctcggtagtg gcggcggcat agtcctcggc tgtgtagtcc tcgagggcgc cattgtcgga
       61 ggtcatggcg tcccagtcgg tcagatcgac atcgctgggg gcgggacgct cggtgtcggg
      121 aagggtgaac acttccgcat cgccctcgga gtcctcggtt tcccacggac ggcggaaacc
      181 cttgtcccgt tcgctgtcgg tccggtcact catacctcga gcaactcttc ttccttgccg
      241 gcaagcagat gatcgatctt gacgacgaaa cgatcggtga gcttctgcaa gtcctcctcg
      301 ccgcggcgga tgtcgtcgtc cgagatctct ccatgcaacg cctccatgtc ggtcttcaga
      361 tgccgccgga tgttacggac ggcgacccgt ccttcctcgg ccatgtgctt cacgaccttg
      421 atcaggtcct tacggcgttc ctcggtgagg ggcgggaaag cgatcctgat caccgacccg
      481 tcgttggtcg ggttgaggcc gagatccgat tgctggatcg cccgctcgat gtcggagacg
      541 gttgacctgt cgaacgggtt cacgaccaac agccgcggct cgggcaccgc gatcgaggcc
      601 aattgctgaa ggggggtcgg agatccgtag tagtcgacgt gaatccgctg caggagctgg
      661 gggttggccc gtccggtcct cacggtggag aactcggtgg cgacgtgatc gattgccatc
      721 tccatcttgt gctcggcctc ggtcaagaga tcgctgatca ttcgccctca cctcctcagt
      781 ggatcaccgt gccgatgcgc tcgccgttga ccgctttcag aatgttgccc ggggcgaaca
      841 tatcgaagac cacgatcggc actccgtgct ccttgcacat cgtaacggcg gtggtgtcca
      901 tgaccctgag ttcgcgggag atgaaatcca tgtaggagag atcgcccatc cggttggcat
      961 ccgagttggt tgccggatcc gcgtcataga tgccgtccac cttgctcgcc ttgagcagga
     1021 cgtcggcgcc gatctccacc gccctgagcg ccgccgtggt gtcggtcgtg attaacgggt
     1081 tgccggtgcc ggcggcaaag atcaccgccc ggcccttctc cagatggcgg atggcgcgga
     1141 gtcggatgta gggctcagcc agttcctgca tggtgatcgc gctctggacc cgggtgggga
     1201 ctccttgctt ctccagggcg tcgcgcaggg ccagggcatt gatcaccgtg gccagcattc
     1261 ccatgtagtc ggcggaggcg ggatccatgc ccttcgccgc ttgggagacc ccgcggaaga
     1321 tgttgccgcc accgaccaca accgcgatct ggaccccggt ggcgtgcgcc gccgcgacct
     1381 cgccggcaac ccggaccaca gtggccgggt cgatgccata gccgatgctc ggatcggcga
     1441 aggcctcgcc ggaaagcttg agcaccaccc gtcgcggcct gtctgtcatt tcgctcttac
     1501 tcaccgacgg ccacccttgc gaacttgcgc accgatatgt tctcacccat cttcgccgcg
     1561 agctgggaga ccaggtcgcc caccgtccct tcgaatttct cggcgttcac gaacttctgg
     1621 tcgtagagca cccggtcggt gtaccaggag ctgagtctcc cctcgacgat gcgctcgacc
     1681 acaccctccg gcttgccctc ggcggatgcc tggcgagcga gcaagtcccg ctctttggca
     1741 acctcgtcgg gatcggcctg ctcccgggtc acccacgacg ggttgcccca gctgatgtgc
     1801 atcgcgatgt cgtcggcggt ctcccgaaat tcctcgctct tggcgacgaa gtcggtctcg
     1861 caggccaact cgaccatcac ccccatcacg ggacgcccgt tctggatatg gatgtaggtc
     1921 ccgatggtgc cttcattggc ctcacggccc gctcgcttgg cgatgctggc ttgtccccgc
     1981 tcccggagca gttcgaacgc cttgtcgaag tcgccatcgg cgtcagtcag ggcctgcttg
     2041 gcgtccatca tcccgacccc ggcatcgacg cggagtttct ggatgtcctt ggcagtgaaa
     2101 tcggccatcg tcgatcagga ctcttcgacg tcgtccgctt tggccgtctt ggcggtggcc
     2161 agctcgcgcc cctcgagggc ggcgtccgcc agagccccgg cgatgagatc ggcggcacgg
     2221 atcgcatcgt cattgcacgg gatcacgtac tcgacctgat ccgggtcgca gttgctgtca
     2281 acgagggcga tcacgggcaa cccgagtctg gaggcttcgg tgacggcggt ggtctccacg
     2341 ttgacgtcga tgacgaagac cgcgtcgggg acccgctgca ggtcggcgac accgcccagg
     2401 ttctgctcga gcttgcccag ttcgcgtcgg agcttgagac gctccttctt gggcagcgag
     2461 ttgatctccc cgctctcctc gagccgacgc aactcgcgca tgtagaagat ccgcttttgg
     2521 atggttgcga aattggtgag catcccgccc aaccaccgga agttgacgta gggcatcccc
     2581 gagcggctcg catgctcggc gaccgcggtc tgcgcctgct tcttggtgcc gacgaagagc
     2641 accgttcccc caccggccac cagatccttg acgtaggcgg tggcgtcgtt gatctgctcc
     2701 agggtctgac gcaggtcgat gatgtgtatc ccgttgcgct cccc
//
LOCUS       DATITW010000741         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_76560, whole genome shotgun sequence.
ACCESSION   DATITW010000741 DATITW010000000
VERSION     DATITW010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76560"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VLS86_10315"
     CDS             complement(<1..201)
                     /locus_tag="VLS86_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkA"
                     /protein_id="HSO50929.1"
                     /translation="MSRIEETKLPGVGIRFDFITQHGQRVGVVHHRGGRREIFICPPE
                     DPDTALMVLDLNDDDVHTLADVL"
     gene            327..1550
                     /locus_tag="VLS86_10320"
     CDS             327..1550
                     /locus_tag="VLS86_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50930.1"
                     /translation="MQRFLLAAAGLLLVGACTTVDDSASSTTAAVTIPPTTGSALAET
                     SGRLVVIDQEGNVVTVRPDGSDQRAVTDDAGESIGYAQPSWSPESDRIAWAEVTAEGF
                     GVGLSDPEGGERSVIPMNAPPFYLYWSPDGQSIGVLHNGAQGAIEFELVDVGGGSAAV
                     VATGSPFYFSWSPESDRVVVHVRGEIFGLIELDGTITDLGSTAVGYQSPHWTPSGIFH
                     RGEQGLELRDVAGAAQTLATARGPLALVANQAGTRVAVQSIADDGPGGVTAAISETPM
                     LPPNSVAVVDVATGEVTQITERPSIGFFWSPDGEKLLVLDPTGEAAEVNALVWDGEET
                     TTLFSFVPHPALVRDVLQFFDQYSQSLQLWSPDSSAVVAVGAVEGEPGIWVHEIEGSG
                     PVDVIDGSWAAWSQQ"
     gene            1689..>2049
                     /locus_tag="VLS86_10325"
     CDS             1689..>2049
                     /locus_tag="VLS86_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010112907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSO50931.1"
                     /translation="MESGDIAWVLTSSALVLFMTPGLALFYGGMVRSRNVLNMLLMSF
                     FTISIVTVVWILVGYSLSFSEGTPLIGDFDFLFLRDVSSGEDLLNVMFQMTFAIITPA
                     LISGAVAERMKFSAWVLF"
BASE COUNT          337 a          653 c          661 g          398 t
ORIGIN      
        1 cagcacgtcg gccagggtgt gcacgtcatc gtcgttcagg tcgagcacca tcaaggcggt
       61 gtcgggatcc tcgggcgggc agatgaagat ctctcttctc cctccacgat gatgcaccac
      121 tccgactcgc tgcccgtgtt gagtgatgaa gtcgaatctg atcccgaccc cggggagctt
      181 ggtctcttcg attctggaca ttcagggcct cctcgatccg gggcatcctt gcagagaatc
      241 ctccgagcca cgaccagagg tggacggagg tcgtgatcga ggaggtcggg ggaacgggca
      301 ggcgtcgatc ggtaccctcc cgtcagatgc agcgcttcct tctcgccgcg gcaggtttgc
      361 tcctggtggg ggcatgcacc acggtcgacg attccgcatc gagtaccacg gccgcggtca
      421 ccatcccacc caccaccggg tccgctctcg cagaaacgtc cgggcggctc gtcgtcatcg
      481 accaggaggg caacgtcgtc actgtccgcc ccgacggctc ggaccagaga gccgtgaccg
      541 acgacgccgg ggagtccatc ggctacgcac aaccctcatg gtcccctgag tcggaccgaa
      601 ttgcatgggc cgaggtcaca gccgagggct ttggtgtggg tctcagcgat cccgaaggag
      661 gcgagcgctc ggtgatcccg atgaacgccc cacccttcta cctgtactgg tcgccggacg
      721 gacagagcat cggtgtgctc cacaacggcg cccagggtgc catcgagttc gagttggtcg
      781 acgtcggcgg cgggtcggcg gcggtggtgg ccactggctc acctttctac ttctcgtgga
      841 gcccggagag cgaccgggtg gtggtccacg tgcgaggcga gatcttcggt ctcatcgagc
      901 tcgacgggac catcaccgat ctggggtcca ccgctgtcgg ctatcagtcc ccccattgga
      961 ctccatcggg gatcttccac cgcggcgagc aaggtctcga gctgcgcgat gtcgctgggg
     1021 cggcccagac cctggccacg gcccgggggc cgctcgccct cgtcgccaac caggctggga
     1081 cccgagtggc ggtgcagtcg atcgcggatg atggccccgg aggcgtcacc gccgcgatat
     1141 ccgagacgcc gatgctcccg ccgaactccg tggccgtagt cgatgtcgcc accggggagg
     1201 taacccagat caccgagcga ccgtcgatcg ggttcttttg gagcccggat ggcgagaagc
     1261 tgctcgtgct cgatccgaca ggggaggccg cagaggtcaa cgcgctggta tgggatggtg
     1321 aggagacgac gacactgttc agcttcgtgc cccatcccgc gctggtgcga gacgtcctgc
     1381 agttcttcga ccagtactcg cagtcgcttc agctctggtc gccggactcc tcggctgtcg
     1441 tcgccgtggg cgccgtggag ggcgagcctg ggatctgggt ccacgaaatc gaggggagtg
     1501 ggccggtgga tgtgatcgac gggagctggg cggcctggtc ccaacaatga ccttgcccaa
     1561 ggcctgagga ccactcgatc cggcatgcgc ccggatccgc cccggcgtta accacttaga
     1621 aacgacctcg aaaccttcct gaaacgaaat ggtgaaactg ttccgtcgtc acgacctgag
     1681 gaggaagcgt ggagtccggt gatatcgcct gggtcttgac cagttccgcg ctggtcttgt
     1741 tcatgacacc ggggcttgcc ctcttctacg gcggcatggt gcggtcgaga aacgtcctca
     1801 acatgctgtt gatgagcttc ttcaccatct cgatcgtgac ggtggtctgg atcctggtcg
     1861 gctacagcct gtccttctcc gaaggcacgc ctttgatcgg ggacttcgac tttctcttcc
     1921 ttcgcgatgt ctcctcgggt gaggatctgc tcaacgtcat gttccagatg acgttcgcga
     1981 tcatcacacc cgccctgatc tcaggcgccg ttgccgagcg gatgaaattc tccgcctggg
     2041 tcctgttca
//
LOCUS       DATITW010000742         1548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_396482, whole genome shotgun sequence.
ACCESSION   DATITW010000742 DATITW010000000
VERSION     DATITW010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396482"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VLS86_10330"
     CDS             <1..480
                     /locus_tag="VLS86_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HSO50932.1"
                     /translation="LLAFPECNSTLEGDDLVLHASHHIGIAVDAADGLLVAVIRDAGG
                     RGVLELAAEVRRLGDGAKARSLAPEELTGQTFTVSNIGAVGGGHGTPIVPYGTTAILS
                     VGRAQDKVIVAGGELEIAPVMPLSLSYDHRVVDGAQGRRFMSLVIENLEEPALFLAG"
     gene            484..729
                     /locus_tag="VLS86_10335"
     CDS             484..729
                     /locus_tag="VLS86_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50933.1"
                     /translation="MPHVHVTPVEEATGVTKGEYDAALARAGRIWNIVSIQSQLPEVM
                     RDSLRLYRSIMFGPSPLSRAQREMIAVVTSNANECHY"
     gene            778..1044
                     /locus_tag="VLS86_10340"
     CDS             778..1044
                     /locus_tag="VLS86_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HSO50934.1"
                     /translation="MVDRFALDWRSCGLDEPTQALLLFAEKLTDECAAISAEDIDDLR
                     AHGFDDQGISSCVQVVAYFNYINRIAEGLGVTAEDWIEDDGRIS"
     gene            complement(1037..>1548)
                     /locus_tag="VLS86_10345"
     CDS             complement(1037..>1548)
                     /locus_tag="VLS86_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HSO50935.1"
                     /translation="KTHLIASDRMGTVIKGYANDDVPIPRDDHGGMETQRLCRRWGAR
                     LTFQSTERYATHHQFTDTPAGTFFCTTHVEAGRDPAHAITLGVAFPDARWFRGRDTEN
                     RQSSTCPDPTCCRVPSAELEDKWSGKVIVSARSQSRILGLLAPDPYPELDMPEVLDLV
                     EKHSDTGLS"
BASE COUNT          252 a          483 c          532 g          281 t
ORIGIN      
        1 ctcctagcct ttcccgagtg caactccacc ctcgaggggg acgacctcgt gctgcacgcc
       61 tcgcatcaca tcggaatcgc ggtcgatgcc gctgatgggc tcctggtggc ggtgattcgg
      121 gacgccggag gtcgcggggt gctcgagctg gccgccgagg tccgacgtct cggtgatggc
      181 gccaaagcaa ggtcactcgc cccggaggag ctgaccggcc agacctttac cgtgtccaac
      241 atcggcgcgg tgggcggggg gcacgggaca ccgatcgtcc cgtacgggac cactgcgatc
      301 ctctcggtgg ggcgggccca ggacaaggtg atcgtggccg gcggcgaatt ggagatagcg
      361 ccggtgatgc cgctctcgct ctcctacgac catcgggtcg tcgacggggc ccagggtcgt
      421 cggttcatgt cgctcgtgat cgagaacctg gaggagccgg cgctgttcct cgccggctga
      481 ccgatgcccc acgttcacgt cacccccgtc gaggaggcaa ccggggtgac caagggggag
      541 tacgacgccg cactggcccg tgcggggcgg atctggaaca tcgtctcgat ccagagtcag
      601 ttgcccgagg tgatgcgtga ctcgcttcgg ctctaccgat cgatcatgtt cggcccttcc
      661 ccgctgagca gggcacagcg ggagatgatc gccgtcgtca cctccaacgc caacgagtgt
      721 cactactgaa tatcggcgca tctacacgac ctccgggatg aggtcgacga tcaggagatg
      781 gtggatcggt tcgccttgga ctggaggagc tgtgggctcg atgagccaac ccaggccctc
      841 ctcctcttcg ccgagaagct gaccgacgag tgcgccgcga tctcggccga agacatcgac
      901 gatctgcggg cccatggctt cgacgaccag gggatctcga gctgtgtcca ggtcgtcgcc
      961 tatttcaact acatcaaccg gattgccgaa ggtctcggag tcaccgcgga ggattggatc
     1021 gaggacgacg ggcggatcag ctgagtccgg tgtctgagtg cttctccacc agatcgagga
     1081 cttcgggcat gtccagctcc gggtagggat cgggggcgag aaggccgagg atccggctct
     1141 gggagcgcgc cgagacgatc accttccccg accacttgtc ctcgagctcc gcggacggga
     1201 cccgacaaca cgtcggatcg gggcaggtcg atgactgccg gttctcggtg tcgcggcccc
     1261 ggaaccaccg tgcgtcgggg aaggccacac ccagggtgat ggcgtgcgcc gggtctctgc
     1321 ccgcttcgac gtgggtggtg cagaagaagg tgccggccgg ggtgtcggtg aactggtggt
     1381 gggtcgcata tctctcggtc gactggaacg tcaaccgcgc cccccatctt ctgcacagtc
     1441 tctgtgtctc catgccgcca tggtcgtcgc ggggaatcgg gacgtcgtcg ttggcgtagc
     1501 ccttgatcac cgtgcccatc cggtcgctgg cgatgagatg ggtcttga
//
LOCUS       DATITW010000743         1695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_597061, whole genome shotgun sequence.
ACCESSION   DATITW010000743 DATITW010000000
VERSION     DATITW010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597061"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VLS86_10350"
     CDS             complement(<1..461)
                     /locus_tag="VLS86_10350"
                     /inference="COORDINATES: protein motif:HMM:NF014128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HSO50936.1"
                     /translation="MRNTSRLIALLTIVAMVATSCGGDDSGPGTGDPATDGTESPEGA
                     TGANCALDEVDGDLNLYNWSEYMDPELITAFEDLHQVDIVEDFYESNEALLAQMQAGA
                     VYDLIVPSDYMVGIMIQNGLLAPINTDAVPNIANLSPRFTELPYDPGPEFSV"
     gene            complement(559..1440)
                     /locus_tag="VLS86_10355"
     CDS             complement(559..1440)
                     /locus_tag="VLS86_10355"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO50937.1"
                     /translation="MSEGTSTTRSTDRILGLNAWLVYLFLYAPILLLIFFSFNDNDNV
                     AIWTEPSLRWYGEMFQDDNVMSALRNSLIVALFATVVSTVIGTMLAIALERYRFRGRG
                     TLDGIAYLPIIIPDVTMAVMLLIFFFQAFGVVDDLFGIQLRNGLPTIILSHIAFSISF
                     VAVVVRARLAQLDNSLEEAAADLYASRWQAFRRVTLPLIAPGVAGGALLALTLSLDDV
                     VVTQFVSGAGATTLPVYVFGLVRRGVTPLINAVSTVMLVASMVLVGISLGFQRGAVAR
                     GQNLADPASLLDDVTAD"
     gene            complement(1437..>1695)
                     /locus_tag="VLS86_10360"
     CDS             complement(1437..>1695)
                     /locus_tag="VLS86_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSO50938.1"
                     /translation="FRKITLPLSMPGVVAGSILVFIPSLGAYVTPAILGGAKTTLLGD
                     YIVSQFLAARNWPFGSALSFAVMFVMLLATIFYFRAGAKTL"
BASE COUNT          340 a          517 c          573 g          265 t
ORIGIN      
        1 accgagaact cgggacccgg gtcgtaagga agctcggtga acctcggcga cagattggcg
       61 atgttgggca cggcatcggt gttgatcggg gcgaggagac cgttctggat catgatcccc
      121 accatgtagt cggacggaac gatcaggtcg tagacggctc cggcctgcat ctgggcgagg
      181 agcgcctcgt tcgactcgta gaagtcctcg acgatgtcga cctggtggag gtcctcgaac
      241 gcagtgatca gctcggggtc catgtactcc gaccagttgt agaggttgag gtcgccgtcg
      301 acctcgtcga gggcgcagtt ggcgccggtg gccccctcgg gcgactccgt gccgtcggtt
      361 gcggggtccc cggtcccagg gccgctgtcg tctccgccac aggatgtcgc caccatggcc
      421 acaattgtca gcaaggcaat cagcctcgag gtgttccgca tctcgtcaat cctcccggga
      481 atatcgatct gccgcgttgg ccgcgccatt taacttatcc ggaccgggag gattcgcaca
      541 tcggcgagtg gggaacgctc agtccgccgt cacgtcgtcg agaagcgagg cgggatcggc
      601 caggttctgc ccccgagcca cggctccgcg ctggaagccg agcgagatcc cgaccaggac
      661 catcgaggcg accagcatca cggtgctgac cgcgttgatc agtggggtga cccctctccg
      721 caccagcccg aacacgtaca cgggcaacgt ggtcgcgcct gcgcccgaga cgaactgggt
      781 gaccaccacg tcgtcgagag acagggtcag cgcgagcagc gccccgcccg cgaccccggg
      841 agctatcaac gggagcgtca cccggcgaaa cgcctgccac cggctggcgt agaggtcggc
      901 ggcagcttcc tcgagggagt tgtcgagctg ggcgagccgg gcgcgaacca ccaccgccac
      961 gaacgagatg ctgaacgcga tgtgggagag gatgatcgtg gggagaccgt tccggagctg
     1021 gatgccgaac aagtcgtcca ccacgccgaa ggcctggaag aagaagatga ggagcatcac
     1081 cgccatggtc acgtccggga tgatgatggg gaggtaggcg atcccgtcca gggtgcctct
     1141 cccccggaac cgatagcgct ccagggcgat ggccagcatg gtcccgatca cggtggagac
     1201 caccgtcgcg aacagggcga cgatcaacga gttcctcagg gcgctcatga cattgtcgtc
     1261 ctgaaacatc tccccgtacc agcgcaacga gggctcggtc cagatggcga cgttgtcgtt
     1321 gtcgttgaac gagaagaaga tcagcagcag gatcggtgcg tagaggaaca ggtacacgag
     1381 ccaggcattg agaccgagga tccggtcggt ggatcgcgtg gtcgaagtcc cctcgctcac
     1441 aacgtcttcg ccccggcgcg gaagtagaag atcgtcgcca gcagcatgac gaacatgacc
     1501 gcgaacgaca acgccgatcc gaacggccag ttcctggccg cgaggaactg gctgacgatg
     1561 tagtccccga gcagggtggt cttcgcccct cccaggatgg caggagtgac gtacgcgccc
     1621 agtgacggga tgaaaacgag gatcgacccg gcgaccacgc cgggcatcga gaggggaaga
     1681 gtgatcttgc ggaat
//
LOCUS       DATITW010000744         1600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_276445, whole genome shotgun sequence.
ACCESSION   DATITW010000744 DATITW010000000
VERSION     DATITW010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276445"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VLS86_10365"
     CDS             <1..297
                     /locus_tag="VLS86_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014280813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HSO50939.1"
                     /translation="VKVAASRQTPALGSAMFAAVAAGTGAGGYASIVEASENMARLGG
                     DEYSPSEESRDVYDDLYGIYRDLHDTYAAQSSEMRRLRSIQRRVQDMSPARVPA"
     gene            294..>1600
                     /gene="araA"
                     /locus_tag="VLS86_10370"
     CDS             294..>1600
                     /gene="araA"
                     /locus_tag="VLS86_10370"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018622590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HSO50940.1"
                     /translation="MKPLDEYTVWFLTGSQVLYGEDVLRLVAENSQAMAKGLDAADAI
                     PVRIVAKPVVTSAESIEEMCRLANSDPGCVGVIAWMHTFSPAKMWIPGLLALDKPLLH
                     LHTQFNRDLPWGEIDMDFMNLNQAAHGDREFAFILSRLRVARKIVVGHWQDPKVADRI
                     GAWSRAACGLHEARNLKVARFGSNMRRVAVTDGDRLEAQVRLGVAVDGFGVADLEMAM
                     SNVAQESIDKLIEEYHDTYDVKPELSPGGGRHESLIEAARIEQGLRDFLEAGGFMAFT
                     DTFEDLAGLPQLPGIAVQRLMADGYGFGAEGDWKTATLVRVMKVMGAGGDRGTSFMEE
                     YTYHLGESGPKVLGAHMLEVCPSISGERPSCEIHPLSIGGKPDPVRLVFTARTGPGVT
                     VGLIDLGNRFRLVANEVELTEPDAPLPNLPVARAVWEPKPDFET"
BASE COUNT          281 a          492 c          548 g          279 t
ORIGIN      
        1 gtcaaggtgg cagcatcgcg tcagaccccg gcgctcgggt cggcgatgtt cgccgcggtg
       61 gccgcgggga ctggagccgg gggctatgcc agcatcgtcg aggcgtccga gaacatggct
      121 cgtctcggtg gggacgagta ctcgccgtcc gaggagtcga gagatgtcta cgacgacctg
      181 tacggcatat accgcgacct ccatgacacc tatgccgccc agtcctcgga gatgcggcga
      241 ttgaggtcga tccagagacg ggtgcaggac atgtcaccag cccgggtgcc ggcgtgaagc
      301 cgctcgacga atacacggtt tggttcctca ccgggagcca ggtcctctac ggagaggacg
      361 tgctccggct ggtggcggag aactcccagg ccatggccaa gggtctcgac gctgcggatg
      421 cgattccggt gcggatcgtc gccaagcccg tcgtcaccag cgccgagtcg atcgaggaga
      481 tgtgccgcct ggccaactcc gacccggggt gcgtcggtgt gatcgcctgg atgcacacct
      541 tctccccggc gaagatgtgg atccccgggc ttctcgcact ggacaagccc ttgctccatc
      601 tccacaccca gttcaaccgg gacctgccct ggggtgagat cgacatggat ttcatgaatc
      661 tcaatcaggc agcccacggt gatcgtgagt tcgcgttcat cctgagccgg ttgcgggtcg
      721 cgaggaagat cgtggtcggg cattggcagg accccaaagt cgcagatcgg atcggcgcct
      781 ggtcccgagc cgcctgtggc ctccatgagg cgcggaatct gaaggttgcc cgattcggga
      841 gcaacatgcg acgggtggcg gtgaccgatg gcgaccggct cgaagcccag gtgaggttgg
      901 gtgttgccgt cgacggattt ggtgttgccg acctcgagat ggcaatgagc aacgtggcgc
      961 aagaatcgat cgacaagctg atcgaggagt accacgacac ctacgacgtg aaaccggagc
     1021 tgagccctgg tggtggccgc catgagtccc tgatcgaggc agccagaatc gaacagggtc
     1081 tgcgcgactt cctcgaagcc ggcgggttca tggccttcac cgacaccttc gaagatctcg
     1141 ccgggctgcc ccagctgcca gggatcgcgg tgcaacgtct catggccgac ggctacgggt
     1201 tcggagcgga gggggattgg aagaccgcca ccctggtacg ggtgatgaag gtcatggggg
     1261 cggggggcga tcggggcacc tcgttcatgg aggagtacac ctaccacctc ggcgagtccg
     1321 ggcccaaggt cctcggtgcc cacatgttgg aggtgtgccc ctcgatctcg ggtgagcgcc
     1381 cgtcgtgtga gattcaccca ctgtccatcg gcgggaaacc ggacccggtc cgcctggtgt
     1441 tcaccgctcg caccggtccc ggggtgaccg tcggcctgat cgatctcggt aaccggttcc
     1501 gcctcgtcgc caacgaagtc gagctcaccg agcctgacgc cccacttccg aacctgccgg
     1561 tggcgagggc tgtctgggag ccgaagccgg acttcgagac
//
LOCUS       DATITW010000745         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_637425, whole genome shotgun sequence.
ACCESSION   DATITW010000745 DATITW010000000
VERSION     DATITW010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637425"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VLS86_10375"
     CDS             <1..657
                     /locus_tag="VLS86_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSO50941.1"
                     /translation="EPGSSWTLLCGNRTANTVMFLEELEGLKDRYPERFQLMHLLSRE
                     GSDLPLLSGRIDEAKVRVVHDQILVGRRVDGWYLCGPYDMVMSARATLSSLGVDADLI
                     HDELFFAGPVDPSTLPPEPPPGEGSIELRVILDGRAVDTRMTAETSILDAALRVRSEL
                     PFSCKGGMCATCKGRIEEGEVRMDKNYALIDSEVEAGYVLTCQSHPVSDRVVVRYDHR
                     "
     gene            657..1232
                     /locus_tag="VLS86_10380"
     CDS             657..1232
                     /locus_tag="VLS86_10380"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSO50942.1"
                     /translation="MVTTRNTRADVVVAAGRLFAEKGYHGTSMRDLGRELGLLGSSLY
                     AHVDSKQDLLVEVVEEGARLFGDSATRALATPGTANDRFRALISGHIEVVLANQDVVR
                     TFLNEARMLDDEHRARVLSARDNYEYAFREVIRQGIGDGSFRDGTDPKMTSIFVLSVL
                     NAVQRWYQPDGDLSPAQLAEAIFQFAVGSLI"
     gene            1366..>2346
                     /locus_tag="VLS86_10385"
     CDS             1366..>2346
                     /locus_tag="VLS86_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HSO50943.1"
                     /translation="MIVAAVLLGAVAPVALAEQRGAFVDDDRSRFEPYIETARSEGLV
                     SGCNPPANDQFCPHRTVTRGEMALMLSRSLQYPAATDDFFADDDGHPAEAAINALAQA
                     GVTRGCGEGRFCPDRSLTRGEMSSLIVGAMRWSLPEDTYPYVDLDDSPYGKSTKTLAR
                     RGALEPCNQPTGNRLCPDRTVARDEAVFSLTSALEMGPSRLSPPEAAAPGLGFADAFE
                     ALDLWDGRVPSARNRVDLTNSGFLGSGLRVTIPEGSHYGADFKLDLSRVVGDEPEHLF
                     FRYFLRLDPDWAPRRSGKLPGFSGVYTQSGKGGYQSTPSAPGWSARMEFFG"
BASE COUNT          363 a          780 c          794 g          409 t
ORIGIN      
        1 gagcccggat cctcgtggac ccttctgtgc ggcaaccgca ccgccaacac cgtcatgttc
       61 ctcgaggagc tggagggatt gaaggaccgc tacccggagc ggttccagct catgcacctc
      121 ctcagtcgcg agggctccga cttgcccctc ctctccgggc gcatcgacga agccaaggtc
      181 cgcgtcgtcc atgaccagat cctcgttggg cgcagggtgg acggctggta cctgtgtggt
      241 ccgtacgaca tggtgatgtc ggcgagggcg accctttcct cgctgggagt cgatgccgac
      301 ctgatccacg acgagttgtt cttcgccggg ccggtcgatc cttcgacgct cccgcccgag
      361 ccaccaccgg gggaggggtc gatcgagctc agggtgattc tcgacggccg cgccgtcgac
      421 accaggatga cggcggagac ctcgatcctc gatgccgccc tccgggtccg gtccgagctc
      481 cccttctcct gcaagggcgg gatgtgcgca acctgcaagg ggcgaatcga ggaaggagag
      541 gtgcggatgg acaagaacta cgcgctgatc gactccgagg tcgaagcggg ctatgtcctc
      601 acctgtcagt cgcatcccgt ctcggaccgg gtggtcgtcc gctacgacca ccgctgatgg
      661 tcaccacccg caacacccgc gccgatgtgg tggtggcagc cgggcgcttg ttcgccgaaa
      721 aggggtatca cgggacttcg atgcgcgacc tcggtcgcga gctggggctc ctcggctcgt
      781 cgctctacgc acatgtcgac tccaagcagg acctcctggt cgaggtggtc gaggaggggg
      841 cgcggctgtt cggggattcg gcgacccgtg cgctggcgac gccaggaacc gccaacgacc
      901 ggttccgggc gctcatcagc ggccacatcg aggtggtcct cgctaaccag gacgtggtca
      961 ggaccttcct caacgaggcg cggatgctcg acgacgagca tcgggcccgg gtgctctccg
     1021 cccgggacaa ctatgagtat gcgtttcgcg aggtgatccg acaggggatc ggggacgggt
     1081 cgtttcggga cggcaccgat cccaagatga cctcgatctt cgtgctttcg gtgctgaacg
     1141 cggtccagcg ctggtatcag ccggacgggg atctgagccc ggcgcagctc gccgaggcca
     1201 tcttccaatt cgccgtcggg agtctcatct gagctcgtgg ggccggcgtc tgcgttgccg
     1261 gtgtcccccg gagccgtagc cccagcgcga aaccccttca ggaaccgggt gcaacagacc
     1321 gacgttgcac cagagatgct ctcgtcgcgt caggctgtca ggtcgatgat cgttgccgcc
     1381 gtcttgctcg gtgccgtggc tcccgtggcc ctggccgagc agcgtggggc gttcgtcgac
     1441 gatgatcgga gcaggttcga gccctacatc gaaaccgcac gctcggaagg tctcgtctcc
     1501 gggtgcaacc ccccggccaa cgaccagttc tgtccgcacc ggacggtgac ccgaggggag
     1561 atggccctga tgctgagccg gtcgttgcag tacccggccg cgaccgatga cttcttcgcc
     1621 gatgacgacg ggcatcccgc ggaggccgcg atcaacgccc tggcccaagc cggtgtcact
     1681 cgcggctgcg gagaagggcg gttctgcccg gaccgcagtc tgaccagggg cgagatgtca
     1741 tcgctgatcg tgggggcgat gcgctggtcg ctccccgagg acacctaccc ctatgtcgac
     1801 ctcgacgact cgccgtatgg caagtcgacc aagaccctgg cgagacgggg cgcgctcgag
     1861 ccatgcaatc agccgaccgg caaccgtctc tgtccggacc ggacggtggc tcgtgacgag
     1921 gcagtgttct cgctgacctc ggccctggag atgggccctt cccggctgag cccgccggag
     1981 gccgccgctc ccggtctggg attcgccgac gctttcgagg ccctcgatct ctgggacggc
     2041 cgtgtcccca gcgcccgaaa ccgggtcgac ctcaccaact cggggttcct cggctcaggg
     2101 ctgcgggtga cgatcccgga ggggtcgcac tatggggcag acttcaagct cgatctgagc
     2161 cgtgtcgtcg gcgacgagcc ggagcatctc ttctttcgct acttcctgcg tctcgacccg
     2221 gattgggcgc ccaggcgcag cggcaagctg cccgggttct ccggggtgta cacccagagc
     2281 gggaagggcg gctatcaatc caccccgtcg gcgccgggat ggtcggcgcg catggagttc
     2341 ttcggg
//
LOCUS       DATITW010000746         4428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_796975, whole genome shotgun sequence.
ACCESSION   DATITW010000746 DATITW010000000
VERSION     DATITW010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796975"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VLS86_10390"
     CDS             <1..937
                     /locus_tag="VLS86_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HSO50944.1"
                     /translation="VQLVQQLLERDDIHFLLPPWGTGFNMAILDIVNEFQTPVLGCTL
                     SSAALAADPGSNQYFYVMLNQPSQQGGALVELLVDLGVESVAIIHHDDLHGIEVNDVV
                     EPALEDAGIETLSNQTYPVNTDDLDQVLRNAQATGAQALLAFSYPAETFLMTGQMRAI
                     GYSPDLFYATVGIAFPVYPGPIAGAGAEGVMGAGAWNPNLDIEGARDYFDRHVAMHGA
                     EPDRWASAACYASGQVLEQAIEEAGTLDPIAVKEAMDTTEFNTILGTFRFENNFNPVY
                     PGQVGQWQNGEFEIISPTSARTAEPIFPKPAWPAG"
     gene            1032..1913
                     /locus_tag="VLS86_10395"
     CDS             1032..1913
                     /locus_tag="VLS86_10395"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSO50945.1"
                     /translation="MDHLLNILIPGLILGGIYALIAVGLSLQYGVGRVLNVSHGEFII
                     LGALITASLFTTAGINPFLVIVIVTPVFFGIGFGIDRTMFNKLRRTSPNDDAFEGRSL
                     LASFGVLFIVQNLMLMYWGPNIIGYSYLTTSVTILGQRYGLNRVTALVVSVVVCVGFL
                     AFIRWTRLGKAIRASAEVPEIADALGVNRKLVMATCFGLGTALAGLAGVLYSLMFDLQ
                     GSRGIELTIIAIVVVVFGGLGSISGSLIGGFALGLLISMVNYFDPSLSLVAFYLVLIL
                     VLLLKPSGILGRQLHAD"
     gene            1903..2904
                     /locus_tag="VLS86_10400"
     CDS             1903..2904
                     /locus_tag="VLS86_10400"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSO50946.1"
                     /translation="MLISTVLSEQKPQLVLIPLSIALLAVAPAYVSSRMLISLITFLM
                     YATLTLSWTMFSAPTNHISLATAGLFGVGVYTSAVLRGSVPIELIVIAGGLIAFALAL
                     AIGLLTLRLRGVYFILFTFGVTALIRNMVQWWETHITGTVGRSVAGATNETVYLLAMI
                     IFSVTLIGLFLLRNSSFGLALQGIGDNQDAAEHLGVDVTRVKVLVFASSAFLMGAMGA
                     VMATRWRYVDPSIAFNPLHSFLPVVMAIFGGTARLYGPIVGTLALFILRDYLITEHPY
                     VYMLIFGVILVVVVLWLPGGLVSQIEKAIFRVKVWWIKTRMWISRQRRLIGVRRAQR"
     gene            2891..3607
                     /locus_tag="VLS86_10405"
     CDS             2891..3607
                     /locus_tag="VLS86_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009804104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50947.1"
                     /translation="MLSGRGVTHSFGGLKAVKNLDFDVSEGEIVGLIGPNGAGKTTLF
                     NLVSGAIPPKEGTIRFKGEDITGLRPDQICHRGLARTFQTTKLFTEMSGFDNVRLALV
                     FGNPGRSFTFKQAEGEANRMMASAGMLGDRNKPVKDLPLASRRRLEIARALATNPDML
                     MLDEAMAGLTAGEVSRSIELVRSLRDRGVTILMIEHVMAAIMEVCDRIVVLHNGEKLA
                     EGTPAEVAEDPKVRSVYLGS"
     gene            3610..4314
                     /locus_tag="VLS86_10410"
     CDS             3610..4314
                     /locus_tag="VLS86_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50948.1"
                     /translation="MLKVNSINSHYGQIQALWDVSLNVEDGEVVVVLGANGAGKTTLV
                     KTIMGLIEPTSGMIKFAGRRIEGRDPHKISRLGVAYGPEGGGLFRDMSVRENLEMGSF
                     NRFAWRRRDTTLERVLRRFPRLEERERTLARNLSGGERQMLVIGRVMMSLPTLCIFDE
                     PSIGLSPVAVDEAFDAIRQLRDEGITVLLIEQNARKGLEIADRAYVLENGRVKMEGDR
                     KSILDEESIKKAYLGL"
BASE COUNT          770 a         1357 c         1432 g          869 t
ORIGIN      
        1 ggtgcagctc gtccaacagc tgttggagag agacgacatc cacttcctcc tcccgccatg
       61 ggggaccggc ttcaacatgg cgatcctcga catagtcaac gagtttcaga caccggtcct
      121 gggttgcact ctgagctcgg ctgccctcgc cgccgatccg gggtccaacc agtacttcta
      181 cgtgatgctc aaccagcctt cccagcaggg aggcgcgctg gtcgagttgc tggtcgacct
      241 gggcgtcgag agtgtggcca tcatccacca cgatgacctc cacggcatcg aggtaaacga
      301 cgtggtcgag cctgcgttgg aggacgccgg aatcgagacc ctgtcaaacc agacttaccc
      361 ggtcaacacc gatgacctcg atcaggtcct ccgcaatgct caggccacgg gggcacaggc
      421 cctgctggcg ttctcctacc cggccgagac cttcctgatg accggccaga tgcgggccat
      481 cggttacagc ccggatctct tctacgcaac cgtgggaatt gccttcccgg tctaccccgg
      541 gcccatcgcc ggcgcaggcg ccgagggggt gatgggggcc ggggcgtgga accccaactt
      601 agacatcgaa ggagcacgcg actacttcga tcgccacgtg gccatgcacg gggcggagcc
      661 cgaccgctgg gcgtcggcag cttgttatgc gagtggtcag gtgctcgagc aggccatcga
      721 ggaggcgggc acgctcgacc cgattgcggt gaaggaagcc atggacacca cggagttcaa
      781 tacgatcctt gggaccttcc gtttcgagaa caacttcaac ccggtctatc ccggccaggt
      841 cggtcagtgg cagaacggcg agttcgagat catctcaccg acatcggctc ggaccgccga
      901 gcccatcttc cccaagccgg cgtggcccgc cgggtaagaa ctgccgtagg ttggttggtg
      961 gcgggctgag gcccgtcacc aaccaccatc aaaggcgata atccacccca ggcgagggct
     1021 gctggcgtcc gatggaccac cttctcaaca tactgatccc gggtctgatc cttggtggga
     1081 tctacgccct gatcgccgtc ggtctcagcc tccaatacgg cgtcggccgg gtgctaaacg
     1141 tgtcccacgg tgagttcatc atcctcgggg cgctgatcac cgccagcttg ttcaccaccg
     1201 cggggatcaa ccctttcctg gtgatcgtga tcgtcacccc ggtcttcttc ggcatcggat
     1261 tcgggatcga ccgaaccatg ttcaacaagc tgaggagaac ctcccccaac gacgacgcct
     1321 tcgagggacg ttcactcctc gcctcctttg gtgtgctgtt catcgtccag aacctgatgt
     1381 tgatgtactg gggaccgaac atcatcggct actcgtacct gaccacctcg gtgaccatcc
     1441 tcggtcagcg gtatgggctc aatcgggtga ccgccctggt cgtctcagtg gtggtgtgcg
     1501 tcggcttttt ggctttcatc aggtggaccc gactgggcaa ggcgatccgg gcttctgccg
     1561 aggttccgga gatcgccgac gccctgggtg tcaatcgaaa gctggtgatg gctacctgct
     1621 tcggtctggg gacggcgctg gccgggctcg ccggggtgct ctacagcttg atgttcgatc
     1681 tccagggctc gcgggggata gagctcacca tcatcgccat cgtggtggtg gtctttggcg
     1741 gtctcgggag catctccggg agcctgatcg ggggattcgc actggggctt ctcatcagca
     1801 tggtcaacta cttcgacccc agcctgtcat tggtcgcttt ctacctggtc ctgatcctcg
     1861 tcctcctcct caagccgtcc gggatcctgg gaagacaact ccatgctgat tagcaccgtc
     1921 ctcagcgagc agaagcccca gctcgtgctg ataccgctga gcatcgccct gctcgcggtg
     1981 gcgccggcgt atgtgtcctc ccggatgctg atcagcctga tcacctttct catgtacgcc
     2041 accctgacct tgagctggac tatgttctcg gcgccaacca accacatctc gttggccaca
     2101 gccgggttgt tcggggtcgg ggtctacacc tcggcggtgt tgcgggggtc ggtccccatc
     2161 gagctgatcg tgatcgcggg agggctgatc gccttcgccc tcgccctggc catcggtctc
     2221 ctcaccctcc ggctcagggg ggtctacttc atcctcttca ccttcggggt gaccgctctg
     2281 atccgcaaca tggtccaatg gtgggagacc cacatcaccg ggacggtggg ccgctcggtg
     2341 gccggggcca ccaacgagac ggtttacctg ctggcgatga tcatcttctc ggtcacgctg
     2401 atcggcctgt tcctcctccg caactcgtcg ttcgggctcg ccctccaggg gatcggcgac
     2461 aaccaggacg ccgccgagca tctcggtgtc gacgtgaccc gggtcaaggt cctggtcttc
     2521 gcctccagcg ccttcctcat gggggcgatg ggggcggtga tggccacccg gtggcgttat
     2581 gtcgaccctt ccatcgcctt caaccccctc cactcgtttc tccctgtagt gatggcgatc
     2641 tttggtggga ccgccaggtt gtacgggccg atcgtgggga ccctggccct cttcatcctg
     2701 cgcgactact tgatcaccga gcatccctac gtctatatgc tcatcttcgg ggtgatcctg
     2761 gtggtggtgg tgctctggct ccccggcggt ctggtgtccc agatcgagaa ggcgatcttc
     2821 agggtcaagg tgtggtggat caagaccagg atgtggatca gccggcaacg gcggctgatc
     2881 ggggtgagac gtgctcagcg gtaggggagt cacccacagc ttcgggggcc tcaaggcggt
     2941 caagaacctc gacttcgacg tctccgaagg tgagatcgtc ggtctgatcg gtcccaacgg
     3001 ggcgggcaag acgacactgt tcaacctggt ctcaggggcg ataccgccca aggaggggac
     3061 gatccggttc aagggtgagg acatcaccgg gttgagaccc gaccagatct gtcaccgggg
     3121 cctggcccgc accttccaga ccaccaagct attcaccgag atgagcggtt tcgacaatgt
     3181 gaggctggca ctggtgttcg ggaacccggg gcgaagcttc accttcaaac aggccgaggg
     3241 agaggccaac cggatgatgg cgtcggcagg catgctcggc gatcggaaca agccggtgaa
     3301 ggacctccct ctggccagcc ggcgtcgact cgagatcgcc agggccctgg ccaccaatcc
     3361 ggacatgctg atgctcgacg aggccatggc cggcttgaca gcaggggagg tctcccggtc
     3421 gatcgagctg gtccgcagtc tccgcgaccg gggagtgaca atcctgatga tcgagcacgt
     3481 gatggcggcg atcatggagg tgtgcgaccg gatcgtcgtc ctccacaacg gtgagaagct
     3541 ggccgagggg acgccagccg aggtcgccga agatcccaag gttcgatcgg tctatctggg
     3601 gagttgacca tgctcaaggt gaacagcatc aactcgcatt acggccaaat ccaggcgctg
     3661 tgggacgtgt cgctgaacgt cgaggatggc gaggtggtgg tggtcctcgg tgccaacggg
     3721 gcgggcaaga ccactctggt caagacgatc atgggtctga tcgagccgac atcggggatg
     3781 atcaagttcg ccgggcggcg gatagaaggc cgagatccgc acaagatcag caggttgggg
     3841 gtggcctacg ggcccgaggg tggagggctg ttccgggaca tgtcggtccg ggagaacctg
     3901 gagatggggt cattcaatcg gttcgcctgg cgaaggcgcg acacaactct ggagagggtg
     3961 ctccgccgtt tccccaggct ggaggagagg gagaggacgt tggcccggaa cctcagtggc
     4021 ggggagcgtc agatgctggt tatcggtcgg gtgatgatgt ccctgcccac tctgtgcatc
     4081 ttcgacgagc catcgatcgg gctctccccg gtggcggtcg atgaggcgtt cgacgccatc
     4141 cgccagctcc gtgacgaggg gatcaccgtg ttgctcatcg agcagaacgc caggaagggc
     4201 ctggagatcg ccgatcgggc gtatgtgctg gagaacgggc gggtcaaaat ggagggtgac
     4261 agaaagtcga tcctcgacga ggagtcgatc aagaaggcgt atctggggct gtgaccggtg
     4321 gcaacgatga cccggtttcc taagcgcacc caggtggtga tcgtcggtgg ttggcccggc
     4381 ccctccctgc tggccctgat gctccacagg gccggcgtgg ctgcgatc
//
LOCUS       DATITW010000747         1620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_676709, whole genome shotgun sequence.
ACCESSION   DATITW010000747 DATITW010000000
VERSION     DATITW010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676709"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1620)
                     /gene="carB"
                     /locus_tag="VLS86_10415"
     CDS             complement(<1..>1620)
                     /gene="carB"
                     /locus_tag="VLS86_10415"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HSO50949.1"
                     /translation="NPRVSRSSALASKATGFPIAKIAALLAVGFTLDEIANDITGATP
                     ASFEPALDYVVVKIPRFDFAKFPSVPTRLGTAMRSVGEAMAIGRTFPEALQKALRSLE
                     NGRAGLNADPGEEAITSVSEDELAQMVAVPSPSRLFAISEALRRGWPVDQVAAMSMVD
                     PWFVDQIAELCEVRAEIELSHLRDSGMLREAKRHGFSDRQLAFLWDKTEDEVRAARKA
                     IGLRRTYKTVDTCAAEFEGVSPYLYGTFEDESEVPPAVRPRVVVLGSGPNRIGQGIEF
                     DYCCVHASFALRDAGYETVMINCNPETVSTDYDTSDRLYFEPLTVEDVLEVIEAESPV
                     AVVVQLGGQTPLNLAHELFEAGVPIAGTSPASIALAEDREQFSELCRSLGLKQPPSGS
                     ASSVTEAVDVVDQVGLPVMVRPSFVLGGRRMKVIYSIEELADYVREIYGEADPELTGA
                     PILIDRFLEAAVEIDVDAVYDGSELLLGGVMEHVEEAGVHSGDSDCVTPPPTISDEAH
                     IEIVSASRELAAALEVRGLINLQFAVKGDEV"
BASE COUNT          291 a          539 c          512 g          278 t
ORIGIN      
        1 agacctcgtc tcccttgacc gcgaattgga ggttgatcaa gcctctcacc tcaagggcgg
       61 ctgccagttc ccgggatgcg gacacgatct ctatgtgtgc ttcgtccgag atcgtcggcg
      121 gaggggtcac gcaatcggaa tcccccgaat gcacaccggc ctcctcgaca tgctccatga
      181 ccccgcccag gagcaactcg ctcccgtcgt agaccgcgtc gacatcgatc tccaccgcag
      241 cttcgagaaa gcgatcgatc aagatcgggg cgccggtgag ctcgggatcg gcttcgccgt
      301 agatctccct gacgtagtcg gcgagctcct cgatgctgta tatgaccttc atcctccggc
      361 cgccgaggac gaaggagggg cgcaccatca ccggcagccc cacctggtcg accacgtcga
      421 cagcctcggt caccgagctg gctgagcccg atggcggctg cttgaggccc aggctccgac
      481 acagctcgga gaattgctcc cgatcctcgg ccagtgcgat ggacgcgggc gaggtcccgg
      541 cgatgggaac cccggcctcg aacaactcgt gggcgagatt gagcggagtc tggccgccca
      601 gctggaccac caccgccacc gggctttccg cctcgatgac ctccaggacg tcttcaacgg
      661 tgagcggttc gaaatacaac cggtccgacg tgtcgtagtc ggtggagacg gtttccgggt
      721 tgcagttgat catcaccgtc tcgtatccgg cgtcccgtag ggcgaatgag gcgtgtacgc
      781 agcagtaatc gaactcgatc ccctgcccga tccggttggg gcctgagccc aggaccacga
      841 cccgcggcct gacggcgggg gggacctcgg attcgtcctc gaaggtgccg tacaggtagg
      901 gagagactcc ctcgaattca gccgcacagg tatccaccgt cttgtaggtg cggcggagac
      961 cgatcgcctt tctcgccgcc cgcacctcgt cctcggtctt gtcccagaga aaggccagct
     1021 ggcggtcgga gaagccgtgt cgcttcgcct cccggagcat ccccgaatcc cgcaaatggg
     1081 aaagctcgat ctcggcgcgg acctcgcaga gctctgcgat ctgatcgacg aaccagggat
     1141 cgaccatgct catggctgcc acctgatcga ccggccaacc ccgccgcaga gcctcgctga
     1201 tcgcaaacaa cctggatggt gacggaacag ccaccatctg ggccagctcg tcctcggaga
     1261 cagaagtgat cgcttcctca ccgggatcgg cgttgagccc ggcccgtccg ttctccaggc
     1321 tgcgcaatgc cttttgcaga gcctcgggga aggtgcggcc gatcgccatc gcctccccca
     1381 cgctgcgcat cgcagtcccc agccgggtcg gcaccgaagg gaacttggca aagtcgaatc
     1441 tcgggatctt caccacgacg tagtcgaggg cgggctcgaa cgaggcaggg gtcgctccgg
     1501 tgatgtcgtt ggcgatctcg tccagggtga agccgaccgc gagcagagcc gcgatcttgg
     1561 cgatcgggaa gccggtcgcc ttggaggcga gcgctgagga gcggctgacc cgggggttca
//
LOCUS       DATITW010000748         1780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_676778, whole genome shotgun sequence.
ACCESSION   DATITW010000748 DATITW010000000
VERSION     DATITW010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676778"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VLS86_10420"
     CDS             <1..129
                     /locus_tag="VLS86_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180902.1"
                     /note="catalyzes the formation of citrate from acetyl-CoA
                     and oxaloacetate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HSO50950.1"
                     /translation="GRLPGWIAQWRELSSDPHARIFRPRQVYVGSPRRDYVPIDER"
     gene            complement(105..689)
                     /locus_tag="VLS86_10425"
     CDS             complement(105..689)
                     /locus_tag="VLS86_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50951.1"
                     /translation="MNEPGEPRVDRSGIFIERDLADSIAIEDELDSSQVGPYRFPEPT
                     RRRTAGWVFVAAALITMLVIDGAWLPVIGLLGLAAWNFWSAWPLAVHEGRALAIAGAA
                     VDFPVGHASAAVTFHGLRSRPRWSVVLYSAEEPPEQRALVVVDAVSGELAEEPYVEPV
                     EALSPGPEGPSIGGSLLQAVADVRRGIYRSSIGT"
     gene            complement(686..>1780)
                     /gene="thrS"
                     /locus_tag="VLS86_10430"
     CDS             complement(686..>1780)
                     /gene="thrS"
                     /locus_tag="VLS86_10430"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HSO50952.1"
                     /translation="IEDHSRELHQRYGFDFVFTPHIAKSDLWRTSGHLDYYAESMFPG
                     MELDNEQEYRVKPMNCPFHVLIYKSRPRSYRDLPIRLSELGAVYRYEMSGVVHGLLRA
                     RGFTQDDSHTFCTEDQLASELALHLDFVLTWLKDVGFTEFEAELSTQPEKSVGDQDRW
                     DFSEKALAATLEDSGLPYRVAEGEGAFYGPKIDIHVKDAIGRGWQLSTIQLDFNNPDR
                     FELEYAASGNVTERPYMIHCAKAGSIERFMGVLIEHYAGALPMWLAPVQVSIIPVADR
                     HQEYADSVAERLRDGQLRVEVDKSDETVGDKIRKALTQKHPAVIVVGDDDVAASTVGL
                     RLYGEDRDSRGVPVIEAANRLVQMAKVPGS"
BASE COUNT          310 a          603 c          566 g          301 t
ORIGIN      
        1 ggccggctgc cggggtggat cgcgcagtgg cgcgagctct cctccgaccc ccacgcccgc
       61 atcttccgac cgagacaggt ctacgtcggc tcgccacggc gcgactacgt gccgatcgac
      121 gagcggtaga tccccctccg gacatcggct accgcctgga ggagactccc tccgatggaa
      181 gggccctccg ggcccgggga caacgcctct accggctcca catatggctc ctcggccagc
      241 tcgccggaca ccgcgtcgac aacgaccagg gcgcgttgct ccgggggctc ctcggccgag
      301 tagagcacca cgctccagcg gggacgggaa cgaagcccgt ggaacgtgac cgcggccgaa
      361 gcgtgcccca cggggaagtc gaccgccgcg ccggcgatgg ccaaagcccg accctcgtgg
      421 acggcgagtg gccatgccga ccagaagttc catgcggcga ggccgaggag gccgatgacc
      481 ggcagccagg ctccgtcgat caccagcatc gtgatcaacg cagccgcgac gaacacccag
      541 cctgcggtcc gtcgacgcgt cggctcggga aaccggtatg ggcccacctg gctggagtcg
      601 agctcgtctt cgattgcgat cgaatcggcg agatccctct cgatgaagat ccctgacctg
      661 tccaccctgg gctcgccggg ctcgttcacg agcccggcac cttggccatc tgcaccaaac
      721 ggttggccgc ctcgatgacc ggcacgccgc gtgagtctcg atcctcgccg tagagcctca
      781 gccccacggt cgacgcggcc acatcgtcgt cgcccaccac gatcacggcc gggtgctttt
      841 gggtgagtgc ctttcggatc ttgtccccga ccgtctcatc ggacttgtcc acctcgaccc
      901 ggagttggcc gtcccgcagc cgctcggcga ccgaatcggc gtattcctga tgacgatcgg
      961 ccaccgggat gatcgagacc tgaaccggcg ccagccacat gggaagagcg ccggcgtaat
     1021 gctcgatgag cacacccatg aacctctcga tcgatcccgc cttggcgcag tggatcatgt
     1081 acgggcgctc agtcacgttt cccgacgccg catattcgag ctcgaagcgg tcggggttgt
     1141 tgaagtcgag ctggatcgtc gagagctgcc acccccgacc gatggcatcc ttgacgtgga
     1201 tatcgatctt ggggccatag aaggcgccct caccctcggc cacccggtag ggaagacccg
     1261 agtcttcgag cgtcgcggcc agggccttct cgctgaagtc ccaacgatcc tggtcgccga
     1321 ccgacttctc cggttgggtg ctcagctcgg cctcgaactc ggtgaatccc acgtccttga
     1381 gccaggtcag gacgaagtcg agatggagcg ccagctcgga ggccagctgg tcctcggtgc
     1441 agaaggtgtg ggagtcgtcc tgggtgaatc ccctggcccg aagcagcccg tggaccaccc
     1501 cggacatctc atacctatac accgccccga gctcggagag acgtatcggc aggtcgcgat
     1561 atgagcgtgg gcggctcttg tagatgagga cgtggaacgg gcaattcatc ggcttgaccc
     1621 gatactcctg ctcgttgtcc agctccatgc ccgggaacat gctctccgcg tagtagtcga
     1681 ggtggcccga ggtccgccac aggtccgatt tggcgatgtg aggggtgaag acgaagtcga
     1741 agccgtatcg ttgatgaagc tcgcgactgt ggtcttcgat
//
LOCUS       DATITW010000749         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_637727, whole genome shotgun sequence.
ACCESSION   DATITW010000749 DATITW010000000
VERSION     DATITW010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637727"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..841
                     /locus_tag="VLS86_10435"
     CDS             131..841
                     /locus_tag="VLS86_10435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSO50953.1"
                     /translation="MGDDSAGVRRGLRTRLSRARNAMFYVVPPRWRGRVAAALPARLA
                     PAPTQPDFFDSFYDSGDPFGFDVNPEEQLKFKKTLDVCGEGVFGRVLEIGCAVGSFTE
                     MIAPRAIEVLAIDVSPAAIEQATRRLRGHSNVRLETRTLPGGFPDGPFDLVIASDVLY
                     YLPVDAVLTCLGQIEAALASGGSLVAVHFVTRMGTMLDGDEVHDILTEHTTMSRTLGE
                     RTEFGPGRPYRIDRYQKA"
     gene            complement(977..>2466)
                     /locus_tag="VLS86_10440"
     CDS             complement(977..>2466)
                     /locus_tag="VLS86_10440"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSO50954.1"
                     /translation="TGRPAQMLYSREQEFISSRTRHPMRVRVKLGAKADGLLHALQMD
                     VLSNTGAYGAHSLTVLSNAGSKTLPLYNKAPHVRFSGDVAYTNLPVAGAYRGYGATQG
                     YFALETAVDRLAAALGIDPLEWRMRNHIREGETSPIFAALGEGREGVEQTITSCALDE
                     CIRQGAERIGWTERRGKRERHGSWVHGVGMSTHMQGSGIPLIDMGSASIKMNDDGSFN
                     LAIGATDLGTGSDTILGQIAAEILAVPLDKIVVTSSDTDLTPFDVGAYASSTTYVSGT
                     AVARAAEDVRAQILEVAGDMLDVEPGSLTLEDERVTAADGTSATLAEVGIRTLYGAEQ
                     RQIAAIHSFTPELSPPPFLAAFCEVAVDEDTGRVVVLRFVAAVDCGTPINPRLAEGQV
                     EGAVANGIGYALFEEMVFDARGRPKTTDYARYKIPGPEDLPRIETILVDSFEPTGPMG
                     AKSVAEIGINAPIPAIANAIHDATGIWLTDTPFTAERVWRALRSR"
BASE COUNT          399 a          789 c          825 g          453 t
ORIGIN      
        1 ccctgcttcc ccaccacgga gagtagccga gctgtcaaac caaaacgcca aggtgagggg
       61 agggcacttc tgggaaccgc gaggtcgaca ctatcgtgag cggtcgctca caccgaaggg
      121 acgtatcgag atgggggacg attccgctgg tgtacgtcgt ggcctgcgta cccggctctc
      181 cagggcccgc aacgcgatgt tctacgtggt gccgccgcga tggcggggac gcgtcgcggc
      241 tgcgcttccg gcgcgcctgg cacccgctcc gactcagcct gatttcttcg acagcttcta
      301 cgactccggc gacccgttcg ggttcgatgt caatccagaa gagcaactca agttcaagaa
      361 gacgctcgac gtgtgtgggg aaggtgtgtt cgggcgagtg ttggagatcg gctgtgccgt
      421 cggctcattc accgagatga tcgccccccg ggccatcgag gtcctcgcca tcgacgtctc
      481 gcccgccgcc atcgaacaag cgactcggcg actgcgcggt cattcaaatg tccgactcga
      541 aacgagaaca ctccccggcg ggtttcccga tgggcctttt gacctcgtca tcgcctccga
      601 tgttctctac taccttccgg tggacgcggt gctcacctgc cttggccaga tcgaggcagc
      661 attggcgagt ggcggtagcc tcgtagccgt tcatttcgtg acgcggatgg ggaccatgct
      721 cgacggcgac gaggtgcatg acatcctgac cgaacacaca acgatgagcc gcacgctcgg
      781 agagaggacc gagttcggac cagggcgtcc ttaccgcatc gatcgttacc agaaagcctg
      841 acgacggctg atgactccca aggagccctc cgggctgatg actcctcgcg atcgccgtgt
      901 ccatcctgga ttgcgctcca ggccaggtgc gactcgactg tccgcggcgg agaccggtcg
      961 ccgccgggcc ccagggtcag cgactccgca gagctcgcca cacccgttcg gcggtgaacg
     1021 gggtgtcggt cagccagatg ccggtggcgt cgtggatggc attggcgatg gcggggatgg
     1081 gggcgttgat cccgatctcc gccaccgact tggcccccat cggcccggtt ggctcgaacg
     1141 aatccaccag gatggtctcg atccggggca ggtcctcggg gccggggatc ttgtagcggg
     1201 cgtagtcggt ggtcttcggt cggccccgag cgtcgaagac catctcctcg aacagggcgt
     1261 agccgatgcc gttggctacc gccccctcga cctgcccttc tgccaatctc gggttgatcg
     1321 gggtgccgca gtcgacggcg gcgacgaagc ggagcaccac cacccggccg gtgtcctcgt
     1381 cgacggccac ctcgcagaag gcggcgagga aaggaggcgg cgagagctcg ggggtgaacg
     1441 agtggatggc tgcgatctgc cgctgctcgg cgccgtagag ggtccgaatc ccgacttcgg
     1501 cgagtgtggc cgatgtcccg tccgctgcgg tcacacgttc gtcctccagg gtgagtgacc
     1561 cgggctcgac atccaacatg tcgccggcca cctcgaggat ctgagcacgt acgtcctcgg
     1621 ccgccctcgc caccgcggtt cccgacacgt aggtggtcga gctggcgtag gccccgacat
     1681 cgaagggtgt taggtcggtg tccgatgaag tgacgacgat cttgtccaac ggcacggcga
     1741 ggatctcggc agcgatctgg ccgaggatgg tgtccgaccc cgttccaaga tcggtggcgc
     1801 ctattgccag gttgaacgac ccgtcgtcgt tcatcttgat ggaggccgat cccatgtcga
     1861 tcagggggat tcccgatccc tgcatgtgag tcgacatgcc gaccccatgg acccaggatc
     1921 cgtggcgttc acgcttgcct cgcctctcgg tccagccgat tctctccgcg ccctgccgga
     1981 tgcactcgtc gagggcacat gaggtgatgg tctgctccac cccttcccgg ccctcgccca
     2041 gcgcggcgaa gatgggggag gtctcccctt cgcggatgtg attgcgcatc cgccactcca
     2101 agggatcgat cccgagggcg gcggcgagcc ggtcgaccgc cgtctccaag gcgaagtagc
     2161 cctgggtggc gccatatccc cgatatgcgc ctgccaccgg gaggttggtg taggcgacgt
     2221 ccccggagaa ccttacgtgg ggcgccttgt tgtacagggg cagggtcttc gatccagcat
     2281 tggagaggac cgtgaggctg tgtgccccgt aggctccggt gttggagagg acgtccatct
     2341 ggagggcatg gagaaggccg tctgctttgg cgccgagctt gacgcggacc cgcatcgggt
     2401 gcctggtccg cgaggagatg aactcctgct cccggctgta cagcatctgg gccgggcgtc
     2461 cggttt
//
LOCUS       DATITW010000750         1522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_196542, whole genome shotgun sequence.
ACCESSION   DATITW010000750 DATITW010000000
VERSION     DATITW010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196542"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VLS86_10445"
     CDS             complement(<1..418)
                     /locus_tag="VLS86_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSO50955.1"
                     /translation="MFDVLETSNFPTEPPLELTAPEDLVDELAAAFRSREREEARVVA
                     LLARAGETKAFERDGFPSLTALLVHRMSLHPGEAQRLIRRANALEEMPLTELAYERGA
                     LSGAQVDVLTETRSVAPEVFTKEEGRLVELAMDTPPV"
     gene            complement(486..1316)
                     /locus_tag="VLS86_10450"
     CDS             complement(486..1316)
                     /locus_tag="VLS86_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017264374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSO50956.1"
                     /translation="MADRFLAGRTAMVTGGASGMGLAMAQAFASQGASVSIGSLLSAG
                     SKTEGEVVHIPGQEQMDHAIAVIEGEGVECLGLDLDVTSDDSVEAFHQATVDRFGTVD
                     ILANAAGITAEQTIVGHPDALWDKVIDVNLTGTYRAIKRVLPGMIDRSWGRIINIAST
                     AATVGAVTSGAYCASKAGVVGLTRCVALEGAPHGVTCNAISPTWVDTSFGREWMSDIA
                     EKQERRSGEEYISDVEKTNPQGRLISPTEIGAMALFLCTDAARGITMEDLRVSAGSLW
                     "
     gene            complement(1344..>1522)
                     /locus_tag="VLS86_10455"
     CDS             complement(1344..>1522)
                     /locus_tag="VLS86_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSO50957.1"
                     /translation="RLEAGTTWINNYNITPIEMPFGGYKQSGIGRENSLVTIDHYTQV
                     KSVYVEMGDVEAPY"
BASE COUNT          279 a          488 c          474 g          281 t
ORIGIN      
        1 gaaccggcgg agtgtccatg gccaattcga ccaggcgccc ctcctctttg gtgaacactt
       61 caggagcgac cgagcgggtc tcggtgagga catcgacctg tgccccgctc aaggctcccc
      121 gctcataggc caattcagtc aatggcattt cttcgagggc gttggctcgg cggattagac
      181 gttgcgcctc accggggtga agcgacatcc gatgaacgag cagagcggtc agagagggaa
      241 aaccgtcccg ttcgaaagcc ttggtctccc cggcccgggc cagaagggcg acgacccgag
      301 cttcctcgcg ttctctgcta cggaaggcgg cggccaactc gtcgaccagg tcttcgggag
      361 cggtcagctc caaaggaggt tcggtgggga agttcgaggt ttcgagaaca tcgaacatat
      421 gttcgactgt accagccacc cccgacaaac tcaagagaat cgcagcagaa atcagagaaa
      481 atctcttacc agagcgatcc agccgagacc ctcaggtcct ccatggtgat gccacgggcg
      541 gcgtcggtgc agaggaacag cgccatggcg ccgatctcgg tcggtgagat gagacggccc
      601 tgtgggttgg tcttctccac gtcggagatg tactcctccc cggaacggcg ctcttgtttc
      661 tcggcgatgt cggacatcca ctcccgcccg aacgaggtgt caacccaggt ggggctgatt
      721 gcgttgcagg tgactccgtg cggagcgccc tcgagggcaa cgcatcgagt gagaccgacc
      781 acgccggcct tggaggcgca gtaggccccg gaggtgaccg ccccgactgt cgccgcggtg
      841 gaggcgatgt tgatgatgcg gccccagctt cgatcgatca tgcccgggag aacccgcttg
      901 atcgcccggt aggtaccggt caggttgacg tcgatgacct tgtcccacag cgcatccggg
      961 tgcccgacga tggtctgctc ggcggtgatg ccggcggcgt tggccaggat gtcgacggta
     1021 ccgaaccggt ccacagtcgc ctgatgaaat gcctcgacgg aatcgtcgga ggtgacgtcg
     1081 agatcgaggc cgagacactc caccccctcc ccctcgatga ccgcgatggc atggtccatc
     1141 tgctcctgac cggggatgtg aaccacttca ccttcggtct tcgaaccggc ggagaggagg
     1201 gacccaatgc tgacactggc accttgcgag gcgaacgcct gagccattgc cagccccatc
     1261 cccgacgccc cacccgtcac catggcggtc ctgcccgcca ggaagcgatc agccacttcc
     1321 tcacctccct ctcggattcc tacctagtac ggcgcctcga cgtcgcccat ctccacgtac
     1381 acgctcttga cctgggtgta gtggtcgatg gtgaccaggc tgttctcccg gccgatcccg
     1441 gactgcttgt agccgccgaa gggcatctcg atcggggtga tgttgtagtt gttgatccag
     1501 gttgtccccg cctcgagccg gg
//
LOCUS       DATITW010000751         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_718132, whole genome shotgun sequence.
ACCESSION   DATITW010000751 DATITW010000000
VERSION     DATITW010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718132"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VLS86_10460"
     CDS             <1..521
                     /locus_tag="VLS86_10460"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSO50958.1"
                     /translation="TQADITATAEVTATLQTSCGELVIQLDPAGFPETVNSFVFLARE
                     GFYDGQVFHRIVADFQLFSGDPLADGSGGPGYRIADELPPEDFVYTPGFVAMDNEGKG
                     TTGSQFFIVLGEAADVLNPQFNVLGEMISGQETLDRIQEVETARRPGSVEDSLPLETV
                     YIESVTIDVTGS"
     gene            558..1406
                     /locus_tag="VLS86_10465"
     CDS             558..1406
                     /locus_tag="VLS86_10465"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HSO50959.1"
                     /translation="MAVDLHSHSTASDGSEKPAALIEQALQIGLTTLALTDHDTQDGI
                     ADAKLAADGTGLELIAGTELSLEYDVGGMHLVVLWLEPGPGPLQDRLLELRHGRDQRN
                     EMMAQRLTELGMPVTAEEILHEGGSGSIGRPHIAAVMMRKGYVETIDEAFELWLTSGK
                     PAYIGRPRLSPEEAIQLARASGSVPVLAHPHTLGINRAHEMADLLTRLIAAGLVGLEA
                     YCAGYRRHEREGYADLARRFGLVPAGGSDFHGTYKPGLSLGTGYGDLIVPDVVVEELR
                     PHAGAA"
     gene            1403..>2437
                     /locus_tag="VLS86_10470"
     CDS             1403..>2437
                     /locus_tag="VLS86_10470"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HSO50960.1"
                     /translation="MSRPLRMGAAAAVVAGSILLSRLLGLAREALLASLIGGNVEGDL
                     YRQAFTIPDFLNYLLAGAYLTITLIPILSRHLEQDDEEGANRAFSSVFRFVALAIGLL
                     TVLMWVAAGPLVSLMFPEAADHDRLVSLTRLVLPAQVFLVMGSLLMAVQYTHRRFVVP
                     ALAPIVYNLGIILGGLASALFGEASPEGFLWGAVAGSAVGNFGLQWFGAKATGTRLVP
                     VPRGQSSIGEYLLLALPLMVGQSIAVLDEQFVRWFGQVEEGATSALSFARQLNMVPVA
                     VIAQAAGVAAYPFLARLAARDDTEGLTATTSKAARNTVFIAAAATAALLVLARPMVRL
                     LYQWGAFTAED"
BASE COUNT          385 a          838 c          786 g          428 t
ORIGIN      
        1 cgacacaagc cgacatcacc gccacggccg aggtgacggc cacgctccag acctcatgtg
       61 gtgagctggt catccaactc gaccctgccg gttttccgga gacggtgaac tcgttcgtgt
      121 tcctggcccg ggaaggcttc tacgacggac aggtcttcca tcgaatcgtc gccgacttcc
      181 agttgttcag cggcgatccc ctcgccgacg gaagcggagg gcctggatac cggatcgccg
      241 acgagttgcc acccgaggac ttcgtctaca cccctgggtt cgtcgccatg gacaacgaag
      301 ggaagggcac caccggaagc cagttcttca tcgttctcgg ggaggcggcc gacgtgctca
      361 acccccaatt caacgtcctc ggggagatga tctcgggcca ggagacgctg gaccggatcc
      421 aggaagtcga gaccgcgcgg cgacccggct ccgtcgagga cagccttccc ctggaaaccg
      481 tctacatcga gagtgtcacc atcgacgtaa ccggttcttg acggtcatac ccgccatcat
      541 cggggtgtag gagacccatg gccgtagacc tccactccca ttcgacggca tccgacggct
      601 cagagaagcc agccgcactg atcgagcagg cgctccagat cgggctcacc accctcgcgc
      661 tgaccgacca cgacacccag gatgggatcg cagacgccaa gctcgccgcc gatggcaccg
      721 ggctcgagtt gatcgcggga accgagctct ctctggagta cgacgtcggg gggatgcacc
      781 tggtggtgct ttggctggag ccgggccccg gccccctcca ggaccggctt ctcgagcttc
      841 gccacggccg ggaccagcgc aacgagatga tggcgcagcg tctgaccgag ttggggatgc
      901 cggtcaccgc cgaggagatc ctccacgagg ggggatcggg gagcatcggt cggccccata
      961 tcgcggcggt catgatgcgc aagggttacg tcgagaccat cgacgaggca ttcgagctgt
     1021 ggctgacatc gggcaagccc gcatacatcg ggcgaccccg actgtccccg gaggaggcga
     1081 tccagctggc tcgtgcctcg ggcagcgtgc cggtgctcgc tcaccctcac acgctgggga
     1141 tcaatcgagc ccacgagatg gcggatctgt tgacccgtct catcgcagcc ggcctcgtcg
     1201 ggctcgaggc gtattgcgcc ggctatcgcc gtcacgagcg ggagggctac gccgacctgg
     1261 cccgacgttt cgggttggtt ccggccggag gcagtgactt ccacggcacc tacaagccag
     1321 ggctgagcct gggcaccgga tacggggacc tgatcgtccc cgatgtcgtc gtcgaagagc
     1381 ttcgccctca cgccggagcg gcatgagccg cccgttgcgc atgggggcgg cggccgcggt
     1441 cgtcgccggg agcattctgc tgtcccggct cctcggtctg gcccgtgagg cgcttctcgc
     1501 ctccctgatc gggggcaacg tggagggcga tctctaccgc caggcgttca ccatccccga
     1561 cttcctgaat tacctcctgg ccggtgccta cctgacgatc accctgatcc cgatcctgag
     1621 ccgtcatctg gaacaggacg acgaggaagg agccaatcgg gccttcagct ccgtctttcg
     1681 cttcgtcgct ttggccatcg gcctgctcac ggtcctgatg tgggtagcgg cgggccccct
     1741 cgtctcgttg atgttcccgg aggccgctga tcacgaccgg ctcgtctctt tgaccaggct
     1801 cgtgctgccc gcccaggtct ttctggtcat gggctccttg ttgatggctg tccagtacac
     1861 ccaccgcagg ttcgtggtgc cggctctggc cccgatcgtc tacaacctcg ggataatcct
     1921 gggaggcctg gcctcagcgt tgttcgggga agccagcccg gaggggttcc tctggggcgc
     1981 ggtggcagga tcggcggtcg ggaacttcgg gctgcagtgg ttcggcgcca aggccaccgg
     2041 cacccgcctc gtccccgtgc cccgggggca atcgtctatc ggcgagtacc tgctgctggc
     2101 actgcccctc atggtgggcc agtcgatcgc ggtgctcgac gagcaattcg ttcgatggtt
     2161 cgggcaggtg gaggaagggg ccacctcggc cctcagcttt gccagacagc tcaacatggt
     2221 cccggtcgcg gtgatcgccc aggcagccgg ggtcgccgcc tatcccttcc tcgcccgtct
     2281 cgcagcccgc gacgacaccg agggcctcac cgccaccaca tccaaggcgg cgcgcaacac
     2341 cgtcttcatc gcggccgccg ccaccgccgc gctcctcgtc ctcgcccgcc cgatggtgag
     2401 gctgctctac cagtggggag cgttcaccgc cgaggac
//
LOCUS       DATITW010000752         1715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_317296, whole genome shotgun sequence.
ACCESSION   DATITW010000752 DATITW010000000
VERSION     DATITW010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317296"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /gene="ccsA"
                     /locus_tag="VLS86_10475"
     CDS             complement(<1..796)
                     /gene="ccsA"
                     /locus_tag="VLS86_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HSO50961.1"
                     /translation="MLAFLGSAGVVAALVGAILLVIRGIGVARGGSSDLIAPSRLLLG
                     GAITAMVAMELALLTDDFSLSYVANHHATETPFPFDVATAWAALEGSIILWGLVLAVF
                     TWVVVRQASDRLGSAAVAVMAGVGVIFFGLMVTVATPFEVCVDVLATGCAASSAWPFT
                     AADVPANGPGPNPLLQNHFLMAVHPPVLYIGYVGLTVPFAYAIGALALGVPGPEWLRR
                     SHRSTLVAWSFLTLGITLGGWWAYEVLSWGGYWAWDPVENASLMPWL"
     gene            complement(796..1203)
                     /locus_tag="VLS86_10480"
     CDS             complement(796..1203)
                     /locus_tag="VLS86_10480"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HSO50962.1"
                     /translation="MDHRRFVYLGIGGVIALVAVLTFSGLNQNLTYYLYPTEAVEQRA
                     DFPDGERFRLAGTVVAGTVVEAADSTSFEVSDGGAAIAVTLTGPPPSLFGEDVPLLLE
                     GAWSGDTFQADTALIRHDENYVVPEEGGAYPDD"
     gene            complement(1303..1575)
                     /locus_tag="VLS86_10485"
     CDS             complement(1303..1575)
                     /locus_tag="VLS86_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50963.1"
                     /translation="MAVVQVPLVYFSVYMFRTLHQTPSIRPDGATLPREMLVALLVNF
                     AVFTYLFVALLIARLEVARAEEGASVEAGLAGDSVKPPRFSEVKDV"
BASE COUNT          351 a          555 c          590 g          219 t
ORIGIN      
        1 cgagccaggg catgagggaa gcgttctcaa cggggtccca ggcccagtaa ccgccccagg
       61 agaggacctc ataagcccac cagccgccca acgtgatccc aagcgtcagg aacgaccatg
      121 cgaccagcgt cgagcggtgg cttcgccgca accactcggg gccggggacg ccgagtgcta
      181 gggctccgat cgcataggcg aacggcaccg tgagcccgac gtagccgatg tacaggaccg
      241 gcgggtggac ggccatgagg aaatggttct gaagtagcgg gttcggcccc ggcccgttgg
      301 cgggaacgtc ggcagcggtg aaaggccagg cgctcgaagc ggcgcacccg gtggcgagaa
      361 cgtccacaca cacctcgaac ggggtggcca cggtgaccat gagaccgaag aaaatgacgc
      421 cgactccagc catcacagcc acggcggccg atcccaagcg atcggacgcc tgccgcacga
      481 ccacccaggt gaagacggcc aggaccaggc cccagaggat gatcgagccc tccagggccg
      541 cccacgccgt ggcgacgtcg aaggggaagg gtgtttccgt ggcgtgatgg ttggcgacat
      601 aggacaggct gaagtcgtcg gtgaggaggg ccagctccat ggcgaccatg gcggtgatcg
      661 cgcctccgag caacaaccgg ctgggcgcga tcagatcgga ggaaccgccc cgggccacgc
      721 cgatgccccg aatcacgagc aggatggcgc cgaccagggc ggcaacgacc ccggccgagc
      781 ccagaaaggc cagcatcagt cgtcggggta ggccccgccc tcctcgggga ccacgtagtt
      841 ctcgtcgtgg cgaatcaggg ccgtgtccgc ctggaaggtg tcacccgacc atgccccctc
      901 gaggagaagg ggcacgtcct ccccgaacaa cgacggcgga ggcccggtca acgtcacggc
      961 gatggcggcg ccaccgtcgg agacctcgaa cgaggtcgag tcagcagcct cgaccacggt
     1021 cccggccacc accgtcccgg cgagacggaa cctctcgccg tcgggaaagt cggcccgctg
     1081 ctcgaccgcc tcggtggggt agaggtagta ggtcaggttc tggttgagac cgctgaaggt
     1141 gagtacggcc acgagagcga tcacgcctcc gattccgagg tagacgaatc gacggtggtc
     1201 caccgtcagc ccttgacccg gcgacgacgc agatggaggt aggcggcgta accgatgatc
     1261 gcggcgtaag agatgacaaa ggtgccgatg acgatgagct gatcagacat ctttcacctc
     1321 ggagaatcgg ggaggcttga ccgagtcacc ggcgagaccg gcttcgactg atgctccttc
     1381 ctcggctcgc gccacttcga gcctggcgat gagaagtgcg acgaacagat aggtgaatac
     1441 ggcgaagttg accaggagcg ccaccagcat ctcacgtggc agcgtggccc cgtcggggcg
     1501 gatcgacggg gtctggtgga gggtgcggaa catgtacacc gagaagtaga cgaggggcac
     1561 ctgcaccact gccaccgctc cgaggatcgc cgagcgccgg gctcgcatct ccgggtcggg
     1621 ggtcgccctg cgcagagcca gatacccgat ctagacgaac agcatcaccg cggtggaagc
     1681 catccgggca tcgccccagt cccaggccct gcccc
//
LOCUS       DATITW010000753         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_718183, whole genome shotgun sequence.
ACCESSION   DATITW010000753 DATITW010000000
VERSION     DATITW010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718183"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VLS86_10490"
     CDS             complement(<1..467)
                     /locus_tag="VLS86_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HSO50964.1"
                     /translation="MPRRGPEQRRKEADIYPPRFRYEAPRSIEEAHTLLTDGGGDAKI
                     LAGGQSLVPMIKLRFASPEMLVDINEIPGLDYHQIDADGSIHVGALCRHADLERSALL
                     ASRQPTMAAAAPLVADPIVRNRGTLVGSLCHADPQGDWASVMVSLDGLVVAQGP"
     gene            complement(448..723)
                     /locus_tag="VLS86_10495"
     CDS             complement(448..723)
                     /locus_tag="VLS86_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50965.1"
                     /translation="MTVEASGHSRGNPRLYELVEHAPKTGKSPTIWAWPQIIDYLKVA
                     GQPVQGPWPPHQKPRPDPGAESLPVAVPDPQSIPPHHTTDPDSAPRT"
     gene            complement(948..1562)
                     /locus_tag="VLS86_10500"
     CDS             complement(948..1562)
                     /locus_tag="VLS86_10500"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HSO50966.1"
                     /translation="MHPLLPVLLAASEGLFPQVDGGVTYFPPHDRGLESIVSFTGHAF
                     VSSRLDEATLADLEPDGFGAVLNPEVQLRMAGAGGIIGVIDVSMVALGQGGGSLPRRG
                     DLDDHPRVRYARSIRDDVAVHGSDSGLVTIGRGLGGRLEMSIEIADNGVRGRDLIQEA
                     LRLVPAGQRLFAAVSPGNARSLRAFLGCGFVPIGSEVIIRPAQT"
     gene            1644..>2185
                     /locus_tag="VLS86_10505"
     CDS             1644..>2185
                     /locus_tag="VLS86_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO50967.1"
                     /translation="MSTAISVSELLKSFGQTRALDGLDLEVATGEVHGFLGPNGAGKT
                     TTIRVLLGLLRHDSGQATLLGGNPWADSTELHRRLAYVPGDVMLWPNLTGGEVIDLLG
                     RLRGGLDQAKRERLLERFDLDPTKKGRTYSKGNRQKVALVAALASNVELLILDEPTAG
                     LDPLMEAEFRNSIEEDRDAGR"
BASE COUNT          379 a          705 c          747 g          354 t
ORIGIN      
        1 gggccttggg caaccaccag gccgtccagt gagaccatga ccgaggccca gtcaccttgg
       61 gggtcggcgt gacagagcga gccgaccaga gtcccccgat tcctcacgat cgggtccgcc
      121 accagtggcg ctgctgccgc catggttggc tggcgggagg cgagcagggc ggaacgctcc
      181 aggtcggcgt gacggcacag cgccccgacg tgtatcgatc cgtcggcatc gatctggtga
      241 tagtccaatc cggggatctc gttgatgtcg accaacatct caggcgaggc aaaccggagc
      301 ttgatcatcg ggaccaggct ctggccaccg gccaggatct tggcatctcc gccgccgtcg
      361 gtgaggagcg tgtgggcctc ctcgatcgac cgaggggcct cgtaacggaa tcgaggggga
      421 tagatgtctg cctccttgcg tcgctgctca ggtccgcggc gcggaatccg ggtcggtggt
      481 gtggtgagga gggatcgact gtgggtcggg cactgccacc gggagcgatt cggccccggg
      541 gtcgggtctc ggcttctggt gtggcggcca cgggccctgg acgggctggc cggcgacctt
      601 gaggtaatcg atgatctgcg gccatgccca gatggtcggg ctcttgccgg tcttgggggc
      661 gtgctccacc aattcgtaca atcgtgggtt gccgcgactg tgcccggaag cctcgactgt
      721 cactgctaac cctccagtct cacgggccga atctcgatga aaacgcggag acgtcgaaca
      781 gcggtgggcg cgtggtagtc gacgttgctc ccatacatag acgcctcctg gccgcggacg
      841 tgctggacgc actgaacgct ccccaacacc ctaaaccgat gaatgcagcc atgccggaaa
      901 aaacgtccag tgtcactgga caccccggca tcccggcctg cgccgtctca ggtctgggcg
      961 gggcggatta tgacttcgct gccgatgggg acgaatccac agccgagaaa ggcgcggagc
     1021 gatctggcgt tgcccgggga gacggcggcg aacagacgct gccctgccgg gaccagacgg
     1081 agtgcctctt ggatcaggtc ccgtcctcgc accccgttgt ctgcgatctc gatgctcatc
     1141 tccagcctgc ctcccagacc ccgcccgatg gtgaccaggc cggagtcgga gccatgcacc
     1201 gcgacatcgt cccggatcga tcgcgcgtag cggacccggg ggtggtcgtc gaggtcaccg
     1261 cggcggggaa gcgacccccc gccctgcccc agagccacca tcgagacgtc gatgacaccg
     1321 atgatcccgc cggctcccgc catgcggagt tggacctccg ggttgaggac ggcaccaaag
     1381 ccgtcaggct cgaggtcggc aagggtcgcc tcgtcgagac gactcgagac gaaggcgtgc
     1441 ccggtgaacg agacaatcga ctccaagccc cggtcgtgag gcgggaaata ggtgacgccg
     1501 ccgtcgacct gggggaacaa gccttcactt gcggcgagga ggaccggcag cagcggatgc
     1561 acggccgaac tatgtcacgc ccccgagtgc gacccaacct ggccgaggag gtccgtggcg
     1621 ccttcgtcgt atcatcctcg atcatgtcta ccgcgatatc ggtctcggaa ttgctcaaga
     1681 gcttcggaca gacccgggcc ctggacgggc tcgacctgga ggtggccacg ggtgaggtgc
     1741 acggcttcct cggccccaac ggagcgggca agacgacgac gatccgcgtt ctgctcggcc
     1801 tgctccgtca cgactccggg caggcgacac ttctcggtgg caacccctgg gccgactcca
     1861 cggaactgca ccggaggctg gcctacgtcc ctggggacgt catgttgtgg cccaacctga
     1921 ccgggggaga ggtcatcgat ctcctgggcc gattgcgcgg cggcctggat caggccaagc
     1981 gggagcgtct cctggagcgg ttcgacctcg accccacgaa aaagggtcgc acctactcaa
     2041 agggaaaccg ccagaaggtg gccctggtcg cggcgcttgc ctccaatgtg gagttgctga
     2101 tcctcgacga gccgaccgcg ggtctcgatc cgctgatgga ggccgagttc cgcaacagca
     2161 tcgaggagga tcgggacgcg ggtcg
//
LOCUS       DATITW010000754         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_37107, whole genome shotgun sequence.
ACCESSION   DATITW010000754 DATITW010000000
VERSION     DATITW010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37107"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..641)
                     /locus_tag="VLS86_10510"
     CDS             complement(30..641)
                     /locus_tag="VLS86_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HSO50968.1"
                     /translation="MSDRDPALALFEFDSISAGIVTGDAMIKAAPLGAIYAGTVHPGR
                     YLVLVTGDTASVEEAQRVGEEVGGDALSDSVFLADVHPAVAGAVRGNSSRATVGDEAI
                     GIVETTTVAAVLDSADAGVKAADAELASIRLADGLGGKAYVIFTGVVAEVEAALEAAV
                     ARTLVHGTLLRSDLIPQLHQEMAENLRRDLHFLNRVSPSRGGR"
     gene            complement(634..2091)
                     /locus_tag="VLS86_10515"
     CDS             complement(634..2091)
                     /locus_tag="VLS86_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSO50969.1"
                     /translation="MINEAEIQAIIERVKGRVAAADVPGRAAPALRASDDLAVSDVQL
                     GDGIHPSIDEAVQAARNAFLGYREMGLEARKTIVEAMRAAMLREGERLGYMAAEETGI
                     GRGEDKAIKNRLVTTKTPGPEDLEPQVVTGDAGMMVTEYAPYGVIGSITPTTNPTSTI
                     INNSISIVSAGNAVTFNVHPNARLVSVENVQLLNRAIVGAGGPPDLVTAIPQPTLDSA
                     RELMNHPEVRVLLVTGGPGVVKEALKTSKKPITAGPGNPPAVVDQTADVEAAGRAIVK
                     GASFDNNVICTDEKTTIVVDTVADRLVQSMVSAGAYRLKEHELKRLERVIFKEMGPPG
                     KPGMINTDWIGKDAAKILSEIGVTVSDEVRLVLAEVPREHNLVWTEQMMPVMPVVRVT
                     NVSDAIDLAVRSEHGFRHTASIHSTNVETITSMARAMNCSIFVANGSNFNGLGQGGEG
                     FTSFSIASPTGEGLTRPRTFSRVRRVTVVGALRIV"
     gene            complement(2094..2405)
                     /locus_tag="VLS86_10520"
     CDS             complement(2094..2405)
                     /locus_tag="VLS86_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HSO50970.1"
                     /translation="MHLGRVAGTLVASRKEALMEGMVFLIVRQLDADNAETGSYVVAV
                     DTVGAGIGEVVMYASGSSARQTELTRDRPVDAVVMAIVDSWDVGGEVRYHKGREEAPT
                     G"
     gene            complement(2442..2744)
                     /locus_tag="VLS86_10525"
     CDS             complement(2442..2744)
                     /locus_tag="VLS86_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HSO50971.1"
                     /translation="MAEALGMIETRSFSAVVEAADAMVKAAKVELVSMEKTGGGYVTA
                     VVRGDVAAVKAAVDAGVRGAEKVGEVIATHVIARPHVNIDLVLPLGRQDQAQEDLG"
BASE COUNT          426 a          991 c          840 g          495 t
ORIGIN      
        1 caccctgccc agcacccgtc ccagctgcat cacctaccac cccgggacgg cgagacccgg
       61 ttgaggaagt ggagatcccg tcgcaggttc tccgccatct cctggtgaag ctgcgggatc
      121 agatcggagc ggaggagtgt cccgtgaacc agggtcctgg ccaccgccgc ttccagcgcg
      181 gcctcgacct cggccaccac cccggtgaag atgacatagg ccttgccccc caggccgtcg
      241 gcaagacgga tcgaagcgag ctcggcgtct gccgccttga ccccggcgtc ggccgagtcg
      301 agcaccgccg ccacagtcgt ggtttcgacg atcccgatcg cctcgtcgcc cacggtcgcc
      361 ctgctgctgt tccccctcac cgccccggcc accgcggggt ggacgtcggc taggaacacg
      421 ctgtcggaga gggcatcgcc cccgacctcc tcgccaaccc tctgcgcctc ctcgacactg
      481 gcagtgtctc ctgtgaccaa caccaggtag cggcccgggt gaacggtgcc ggcatagatc
      541 gcccccagag gcgcggcctt gatcatcgcg tcgccggtga cgatgccggc gctgatcgaa
      601 tcgaactcga agagggcgag agcaggatcg cgatcagaca atgcgcaggg ctccaaccac
      661 ggtcacccgg cgtacccggg agaaggtgcg tggccgggtc aacccttccc cggtgggact
      721 ggcgatcgag aacgaggtga acccctcccc tccctggccg agcccgttga agttggagcc
      781 gttggcaacg aagatgctgc agttcatggc cctcgccatg ctggtgatgg tctcgacgtt
      841 ggtgctgtgt atcgacgccg tgtgcctgaa accgtgctcg gagcggactg cgagatcgat
      901 ggcgtcgctg acgttggtca cccgcaccac gggcatcacc ggcatcatct gctcggtcca
      961 gaccaggttg tgctcacgcg gcacttcggc aaggaccagt ctcacctcgt cgctcacggt
     1021 cacaccgatc tcggagagga tcttcgccgc gtccttgccg atccagtcgg tgttgatcat
     1081 ccccggcttg ccgggtgggc ccatctcctt gaagatcacc ctctccagac gcttcagctc
     1141 gtgctccttg agccgatagg cgccggcgga gaccatcgac tgcaccagac ggtcggccac
     1201 cgtgtccacc acgatcgtgg tcttctcatc ggtgcagatg acgttgttgt cgaaggaagc
     1261 acccttgacg atggcccgtc ccgcggcctc cacgtcggcg gtctggtcga ccactgcggg
     1321 gggattgccg ggcccggccg tgatcggctt cttgctggtc ttcagcgcct ctttcaccac
     1381 gccggggccc ccggtcacca gcaacacccg gacctcgggg tggttcatca gctcccgcgc
     1441 cgaatcgagg gtcggttgcg ggatggccgt caccagatcg ggtgggccac cggcaccgac
     1501 gatggcccga ttgagcagct ggacgttctc cacggagacc aatcgggcgt tggggtggac
     1561 gttgaacgtg accgcgttgc ccgccgagac gatcgagatc gagttgttga tgatggtgga
     1621 ggtcgggttg gtggtgggtg tgatcgaacc gatcacaccg taaggggcgt actcggtgac
     1681 catcatcccc gcatccccgg tcaccacctg tggctcgagg tcctcggggc ccggggtctt
     1741 ggtggtgacg agacggttct tgatcgcctt gtcctcgccg cggccgatcc ccgtctcctc
     1801 ggctgccatg tagccgagtc gctccccttc gcgcagcatc gcggctcgca tcgcctcgac
     1861 gattgtcttc cgagcctcca gacccatctc cctatacccg aggaaggcgt ttctggccgc
     1921 ctgaaccgcc tcatcgatcg aggggtggat cccgtcaccg agttggacat ccgacactgc
     1981 gagatcgtcg gaggcgcgca acgcaggagc cgcccgcccg ggcacgtcgg cggcggcgac
     2041 ccggcccttg acccgctcga tgattgcctg aatctctgcc tcgttgatca tcgctacccg
     2101 gtgggtgcct cctcccggcc tttgtggtag cggacctctc ccccgacgtc ccaggagtcg
     2161 acgatcgcca tcaccacggc gtcgaccgga cggtcgcggg tcagctccgt ctggcgcgcc
     2221 gagctgcccg aggcgtacat gaccacctcg ccgattccag ccccgacggt gtcgaccgcg
     2281 accacgtaac tcccggtctc cgcattgtcg gcgtcgagtt ggcgcacgat gagaaacacc
     2341 atgccctcca tcagcgcctc tttgcggctg gcgaccaacg taccggcgac cctgcctaga
     2401 tgcacggccc tatctcctga ctcgctcccg atgcagtcgg actatcccag atcttcctgt
     2461 gcctggtcct gacgcccgag cggcagcacc aggtcgatgt tcacgtgcgg gcgcgcgatc
     2521 acgtgggtgg cgatcacctc gcccaccttc tccgctcccc ggacaccggc gtccaccgcg
     2581 gccttcaccg cggcaacgtc gccgcgcacc accgcagtca cgtatccgcc gccggtcttc
     2641 tccatgctca ccagctccac cttggcggcc ttgaccatgg cgtcggccgc ctcgaccacc
     2701 gccgagaagc ttcgcgtctc gatcattccc agagcctcag ccatgaagct cc
//
LOCUS       DATITW010000755         4815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_797579, whole genome shotgun sequence.
ACCESSION   DATITW010000755 DATITW010000000
VERSION     DATITW010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797579"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1170)
                     /gene="metX"
                     /locus_tag="VLS86_10530"
     CDS             complement(76..1170)
                     /gene="metX"
                     /locus_tag="VLS86_10530"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HSO50972.1"
                     /translation="MTYPVSDETRYFDLPSVFSMESGETLTGARVAFRTWGKPRPEAT
                     LICHALTGSADADVWWEGLFGPSRSFDPEHDFIIASNVLGGCYGTTGPTSPKPGTETW
                     YGNDFPRVTIRDMVNLQAQLLDHLDVDSLRLVIGGSMGGMQALEWAAAFPDRVKGVVA
                     IGVGAHHSAWSVAFSEAQRAAIWADPKFREGDYGPEDGPDNGLSVARMIAMISYRGKD
                     NFETRFGRNTVEDEFEIHKYLRHQGQKLVDRFDANTYLRLMDAMDSHDLARDRGDPAL
                     VLAGIKTAVLTVGISSDILYPASEVEALAVALPNARYQMLHAPQGHDAFLIETANLDG
                     IVSRFKNDLARGITPADDLSTGRGKGAAWA"
     gene            complement(1167..2486)
                     /locus_tag="VLS86_10535"
     CDS             complement(1167..2486)
                     /locus_tag="VLS86_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HSO50973.1"
                     /translation="MSTEYRFETLQVHAGQEPAPGTNARAVPIYQTTSYTFDDAGHGA
                     RLFALQEFGNIYTRIMNPTTDVFEKRIAALEGGVAAVATSSGQAAQLTAILTIAEAGD
                     NIVSTSFLYGGTYNQFKVALPRLGIEVRFVDGDDSAQFEALIDDRTKAVYLETIGNPQ
                     FNVPDIPAIAEKAHALGVPVIVDNTFGAAGYLSRPIEHGADIVVASATKWIGGHGTSI
                     GGVIVDSGNFDWRNGRFPIFTEPAPGYHGLVFSDVFGPDGSFGNIAFAIRARVEGLRD
                     LGAAISPFNSWLFIQGLETLSLRVQRHVDNAMELARWLESHDSVTWVDYPGLESHAYH
                     ENAKRLLRNGFGSVLTFGIVGGDEPARKFIDSVELASHLANVGDAKTLVIHPYSTTHQ
                     QLSEEEQRAAGVRSDQIRVSVGIEHIDDIIADFEQAFATATEKEPVA"
     gene            2820..3821
                     /locus_tag="VLS86_10540"
     CDS             2820..3821
                     /locus_tag="VLS86_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006135026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HSO50974.1"
                     /translation="MDFGVVLQTDPPAQRVVELAKKAEGLGFSHVWTFDSHVLWQEPF
                     VIYSRILAETEHMVVGPMVTNPGTRDSTVLASLFATLNDMYGPRTICGMGRGDSALRY
                     IGRPPKTLEAMVEAMDVIKRLVAGEEIQYNGQPLSIPWIKEGWDLPIWVAGYGPRALA
                     TVGEHADGFILQLADPQVLQWTVAAVREAAVKAGRDPDQVKICVVAPAYVGEDLAHQR
                     DQLRWFGGMVGNHVVDMVNRYGEDEAKVPKVLSDYIKARESYDYDHHGKSGNPSTDFV
                     PDEIVDRFCVLGPVEDHIEKLSLLKSLGVDHFGIYLMHDDMDGTLDAYGEKVIAAIG"
     gene            3881..>4815
                     /locus_tag="VLS86_10545"
     CDS             3881..>4815
                     /locus_tag="VLS86_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSO50975.1"
                     /translation="MTSMSLPTGTVTFLFTDIEGSTRLVQQLAEAWVPVLESHNRVVA
                     EAIESNAGIVVKTEGDSFFAVFAHATDGLQAALAAQRALMAHSWPSDGVVRSRMGLHT
                     GLGALGAADYVGIDVHRAARIADAAHGGQTILSEPTAVLVERELPAGVRLRDLGKHRL
                     KDLSEPEAIFQVVADGLQSEFPLLRTLDAIPNNLPKQLTSFIGRDRELALAIRLLEGT
                     RVLTLTGPGGTGKTRLSLQVAAEAADAFSDGVFFVELAPVTDIEVVPSSILETLGIRA
                     STRDQSPAERLMSQLSAKSVLLVLDNFEQLLDAAPL"
BASE COUNT          822 a         1525 c         1587 g          881 t
ORIGIN      
        1 ggaggtctag ggggagagcg gacatcgggg aggcaaccta acagtggagg attggcgtcc
       61 agggtgagcc tgtcgtcagg cccaagcggc gcctttgccc cttccggtgg agaggtcgtc
      121 ggcgggcgtg atccctcgcg ccaggtcgtt cttgaaccgg ctgacgatgc cgtcgaggtt
      181 ggcggtctct atgaggaagg catcgtgccc ttgtggcgca tggagcatct gataccgggc
      241 gttggggagg gcgacggcca gggcctcgac ctcggatgcc ggatagagga tgtcgctgga
      301 tatgccgacg gtcaacaccg cggtcttgat cccggcgaga accaatgccg ggtctccccg
      361 gtcccgggcg aggtcatggc tgtccatggc atccatcagc cgcaggtagg tgttggcatc
      421 gaagcgatcg accagtttct gaccctgatg acggaggtac ttgtgaatct cgaactcgtc
      481 ctcgaccgtg ttgcggccga agcgggtctc gaagttgtcc ttcccccgat aggagatcat
      541 tgcgatcatc cgggcaaccg acaggccatt gtccggtccg tcctcgggcc cgtagtcgcc
      601 ctcacggaac ttggggtcgg cccagatggc ggcacgctgc gcttcggaga aggccacgct
      661 ccatgccgag tgatgggcgc caacccctat ggcaaccacg cccttcaccc ggtcggggaa
      721 agccgccgcc cactccaggg cctgcatccc ccccatcgag ccgccgatca cgagacgcag
      781 gctgtccacg tcgagatggt cgagcagctg ggcctggagg ttgaccatgt ccctgatcgt
      841 gaccctcggg aagtcgttcc cgtaccaggt ctccgttccc ggcttgggcg acgtgggccc
      901 ggtggtgccg tagcaaccac cgaggacatt gctggcgatg atgaagtcat gctcggggtc
      961 gaacgagcgg gatggcccga acaggccctc ccaccaaacg tcggcgtcgg cgcttccggt
     1021 cagggcatga cagatcagag tcgcctcggg tcggggcttt ccccaggtgc gaaaggcgac
     1081 acgagccccg gtgagtgtct ccccgctctc catggagaag acactgggga ggtcgaagta
     1141 ccgcgtttcg tccgaaaccg ggtaggtcaa gcgactggct ccttctcggt cgccgtggcg
     1201 aatgcctgct cgaagtcggc gatgatgtcg tcgatgtgct cgatcccgac cgagaccctg
     1261 atctgatccg accggacccc ggccgcccgc tgctcctcct cggagagctg ctgatgggtc
     1321 gtcgagtagg ggtggatcac cagtgtcttg gcgtcaccga cgttcgccag atgtgaggcc
     1381 agttcgaccg agtcgatgaa cttgcgcgcc ggttcgtcac caccgactat gccgaaggtc
     1441 aacaccgacc cgaagccgtt gcggagcagg cgcttggcgt tctcgtggta ggcatggctc
     1501 tccagcccgg gatagtcgac ccaggtgacc gagtcgtgcg actcgagcca tctcgccagt
     1561 tccatcgcat tgtcaacatg tcgctgcacc cgcagagaca gtgtctccag gccctggatg
     1621 aacagccacg agttgaacgg gctgatggcc gcccccaggt ctcgcagacc ttccacccgg
     1681 gcccggatcg cgaaggcgat gttgccaaaa gacccatcgg ggccgaacac gtcggagaac
     1741 accagcccgt gatagcccgg ggcgggctcg gtgaagatcg ggaatcggcc attgcgccag
     1801 tcgaagttgc ccgagtcgac gatgacgcct ccgatcgagg tcccatgtcc gccaatccac
     1861 ttggtggccg aggcgacgac gatgtccgct ccgtgctcga ttgggcggct gagatagccg
     1921 gcggcgccga acgtgttgtc gacgatgacc ggcaccccga gggcgtgcgc cttctcggcg
     1981 atggccggga tgtccgggac attgaattgg gggttgccga tggtctccag atacacggcc
     2041 ttggtccggt cgtcgatcag cgcctcgaat tgagcggagt cgtcgccgtc gacgaatcga
     2101 acctcgatgc ccaacctggg tagggcgacc ttgaactggt tgtaggttcc cccgtacagg
     2161 aagcttgtcg acacgatgtt gtccccggcc tctgcgatgg tgaggatcgc agtgagctgc
     2221 gccgcctgac cactcgaggt ggccaccgcg gccacacccc cctcgagggc ggcgatgcgc
     2281 ttctcgaaga cgtccgtggt cgggttcatg atccgggtgt agatgttccc gaactcctgc
     2341 aatgcgaaga gtcgggcgcc gtggccggcg tcatcgaagg tgtacgacgt cgtctggtag
     2401 atggggacgg cccgggcgtt ggtgcccggc gcaggttcct ggccggcatg gacctgaagg
     2461 gtctcgaatc ggtactcggt ggacattcgc agttctcctc tggcgaggat tcgccccgtc
     2521 aaccgccccg gaatcgaaaa gacccgcgac cgtcgccggt gcggatccca tcttccgggt
     2581 ctgtggcgct gctacgcgtc tcgtccgcac ggctcgtcgt gagccatgca catacaagtg
     2641 gtggtccgtg cagcgaacat gtgagacagg aaggtatcgg agacccatca ccccgtcaac
     2701 ccgtccccga ccgtaatcgg gacggtgaag ggccggatat gacccctggt ggtcccaatt
     2761 acaggaggcc gtgtgcagta actttgagtg atgaaccaga tgacggctcc ggtccgttga
     2821 tggacttcgg cgtcgttctc caaaccgacc ccccggccca gcgggtagtc gaactggcga
     2881 agaaggccga ggggctcggc ttcagccatg tctggacgtt cgactcccat gtcctgtggc
     2941 aggagccctt cgtcatctac tcgcggatcc tggccgagac cgagcacatg gtggtggggc
     3001 ccatggtcac caacccgggc accagggact cgaccgtgct cgcctcgctg ttcgccaccc
     3061 tcaatgacat gtacggcccc cggaccattt gtggtatggg gcggggcgac tccgcccttc
     3121 gctacatcgg ccgtcccccc aagacgctcg aggcgatggt ggaggccatg gacgtgatta
     3181 agcgtctggt cgccggtgag gagatccagt acaacggaca gccgttgagc attccctgga
     3241 tcaaggaggg ttgggacctg ccgatctggg tcgccgggta cggcccgaga gccctggcca
     3301 ctgtcggcga gcatgccgac gggttcatac tccagctcgc cgacccccag gtgctgcagt
     3361 ggaccgtcgc cgcggtgcgc gaagccgcgg tgaaggccgg aagggacccc gaccaggtca
     3421 agatatgcgt ggtcgcaccc gcttatgtcg gtgaggatct ggcccaccag cgggaccagt
     3481 tgcgctggtt cggtgggatg gtcggcaatc acgtcgtgga catggtgaac cggtacgggg
     3541 aagacgaggc gaaggtgccc aaggtcctct ccgactacat caaggcgcga gagtcttacg
     3601 attacgacca ccacggcaag tcgggcaacc cttccaccga cttcgtcccc gacgagatcg
     3661 tcgaccggtt ctgtgtcctc gggccggtgg aggatcacat cgagaagctc tcgttgctga
     3721 agtcgttggg cgtcgatcac ttcggcatct atttgatgca cgacgacatg gacggcaccc
     3781 tcgatgccta cggcgagaag gtcatcgcgg cgatcgggtg agccgggtcg ggctcaacta
     3841 tcttctccct gtgcattcca ggtccggtgg tgtctgagtc atgacctcca tgtcgctgcc
     3901 cacgggcacg gtgacctttc tcttcaccga catcgagggc tcgacccgcc tggtccaaca
     3961 gctcgcggag gcttgggtcc cggtactcga atcccacaat cgggtcgtcg ccgaggccat
     4021 cgagagcaat gcggggatcg tcgtcaagac cgagggtgac tccttcttcg ccgtgtttgc
     4081 ccacgccacc gacggcttgc aggccgctct cgccgcccaa cgcgcactga tggcccattc
     4141 ctggcccagc gacggggtgg tcaggtcccg gatgggcctg cacactgggc tcggcgccct
     4201 gggtgcggcg gactacgtcg ggatcgacgt gcatcgggcg gcccgcatcg cagacgcggc
     4261 acacggtggt cagacgatcc tctccgagcc gaccgccgtg ctggtggaga gggaactgcc
     4321 cgcaggagtg cggcttcggg atctggggaa gcatcggctg aaggaccttt ccgagcccga
     4381 ggcgatattc caggtggtgg ccgatggcct ccagtcggag ttcccgttgc tgagaacgct
     4441 cgacgccatc cccaacaacc tccccaagca gctcaccagc ttcatcggac gggatcgtga
     4501 gcttgcgttg gcgattcgtc tgctggaagg gacacgggtc ctcaccctca ccggcccggg
     4561 agggaccggc aagactcggc tttcgctcca ggtcgcggcg gaagcggcgg atgccttctc
     4621 cgacggggtg ttcttcgtcg agctggcccc ggtcaccgac atcgaggtcg tcccctcgag
     4681 catcctcgag actctcggga tccgggcctc cactcgtgac caatcacctg cggagcggct
     4741 catgagccag ctctcggcca agagcgtgct cctcgtcctg gacaacttcg agcagcttct
     4801 cgacgcggcg ccgct
//
LOCUS       DATITW010000756         2991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_156806, whole genome shotgun sequence.
ACCESSION   DATITW010000756 DATITW010000000
VERSION     DATITW010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156806"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VLS86_10550"
     CDS             <1..185
                     /locus_tag="VLS86_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HSO50976.1"
                     /translation="VSRIIKVAAAYDWHVYQDRLSPLQALENLHAGAAYEYDPEVVAS
                     LRRLLESRGVLIPARA"
     gene            complement(186..776)
                     /locus_tag="VLS86_10555"
     CDS             complement(186..776)
                     /locus_tag="VLS86_10555"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HSO50977.1"
                     /translation="MLWLAIVLFIALAIAVLRGGRLTNLGEVELRAWWLLVIALALQF
                     GTSLLPDTDWAEGLGLAMVLSSFLLLMALVLLNRNRTGMWLAGLGVLMNFTVIGLNGG
                     MPVLAEAAEVASGFTVTAPDLSGSFKHVILDESSRLTMLADVIPLRLGALGQVISLGD
                     VFLAVGLGRFLEQELRRPRRYFRKGAHAQPGSASKP"
     gene            775..1584
                     /locus_tag="VLS86_10560"
     CDS             775..1584
                     /locus_tag="VLS86_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSO50978.1"
                     /translation="MRSDGNTSRPASKESDLIRYEVVEGRATITIDDPERRNPLSTDT
                     MAGLVDATRRALDDPEVRVLVFTGAGDQAFSAGGDLSGSFVDDPVGLHRARGALADLF
                     RLMYRGGKPTVARVNGHALAGGFGLAVACDVTICVDDAMLGTTEVKVGLWPMMISAVL
                     IRVMPRKAVLDLMLTGRIIDPLEALRLGAVSRVVARADLDDAVSEVVEALKTSSPATL
                     MIGRDAFFGIADLDFDAALDRLQTGLTATAMTEDAAEGLAAFREKREPRWK"
     gene            1575..2291
                     /locus_tag="VLS86_10565"
     CDS             1575..2291
                     /locus_tag="VLS86_10565"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HSO50979.1"
                     /translation="MEVKTAPVATRSGWARAVLGWTLIWSGLFIFGYLGWQLFVTDLV
                     NARVQATAVVELDETLEEAREDPPVVEEVEADDGVVVEFYPEEAPAEDEEFAMIRVPS
                     LGLEAVVFEGVSLDTLSKGPGHMPGTPLPGQPGNAVISGHRTTHGRPFYDFDLLVSGD
                     RIEVETALGRHVYEVRDSFVVEPTDVWVADDRSGGWLTLTTCNPKFSARERLIIVAEM
                     VEGPNLEYVNLIEDGVGGLA"
     gene            complement(2288..2743)
                     /locus_tag="VLS86_10570"
     CDS             complement(2288..2743)
                     /locus_tag="VLS86_10570"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSO50980.1"
                     /translation="MFGLALSWVLDGGGDIATEGDAAPDFSVELFNGSTFSLQGHLED
                     DGRPVLLNLWASWCAPCREEIPALSDFAVRNPEIAVIGVAVEDTLDDALAFAAELEPS
                     DPLGLGGDDFETAYPNFGLPVTYFIDADGVIREVFNGILTEESLQGLIP"
     gene            complement(2808..>2991)
                     /locus_tag="VLS86_10575"
     CDS             complement(2808..>2991)
                     /locus_tag="VLS86_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50981.1"
                     /translation="FDIEIDDGLQPRQSVLVRATAQDDSRKEFHTIARVDTPIEVEYL
                     RNGGILHYVLRKMASA"
BASE COUNT          526 a          967 c          999 g          499 t
ORIGIN      
        1 tcgtgtcccg gatcatcaag gtggccgctg cctacgactg gcacgtgtac caggatcgtc
       61 tctcaccgct gcaggccctg gagaatctcc atgccggcgc cgcctatgag tacgatcccg
      121 aggtcgtcgc ctcgttgcgg cgtctcctcg agagccgggg cgttctcatc ccggcccgcg
      181 cttgatcagg gtttgctggc cgagcccggc tgggcgtgtg cgcccttcct gaaatagcga
      241 cggggccgtc tcagctcctg ttccaggaac cggcccaacc ccaccgccag gaacacgtcg
      301 ccgagactta tcacctgtcc cagcgcaccg agacggagcg ggatgacgtc ggccagcatg
      361 gtcagtcgcg agctctcgtc caggatcacg tgcttgaacg acccggagag gtcgggtgcg
      421 gtgacggtga acccgctcgc cacctccgcc gcttcggcca acaccggcat tcccccgttg
      481 aggccgatca cggtgaagtt catcaggaca ccgagacccg ccaaccacat cccggtccgg
      541 ttgcggttga gcagcaccaa cgccatcagc aacaggaagg aggagaggac cattgccagc
      601 ccgagaccct cggcccagtc tgtgtccggg aggagagacg ttccgaactg aagggcgagc
      661 gctatcacta gcagccacca ggcacgaagc tctacttcac cgaggttggt cagccggccg
      721 ccgcgtagca ccgctatagc gagagcaatg aagaggacga tggccagcca tagcatgagg
      781 tcggacggta ataccagccg ccccgcatcg aaagagagcg acttgatcag gtacgaagta
      841 gtcgagggca gggccaccat caccatcgac gaccccgagc gtcgcaaccc cctgtcgacc
      901 gacaccatgg ccggcctggt cgacgccacg cgccgcgcac tcgacgatcc cgaggtccgg
      961 gtcctggtct tcactggcgc cggggatcag gccttctcgg ccggtggcga tctctccggt
     1021 tcgttcgtgg acgacccggt gggcctgcac cgggcgaggg gcgctctcgc cgatctgttc
     1081 cgtctcatgt atcggggggg caagcccacc gttgcccggg tcaacgggca tgctctggcc
     1141 ggtggcttcg gactcgcggt ggcctgcgat gtcacgattt gtgtcgacga cgccatgctg
     1201 gggacgacgg aggtcaaggt cgggctttgg ccgatgatga tctcggccgt cctgatcagg
     1261 gtcatgcccc gcaaggcggt cctcgacttg atgctcaccg ggcggatcat cgaccccctc
     1321 gaggccctgc ggctgggtgc ggtgtcgaga gtcgtcgcca gggcggacct cgacgatgcg
     1381 gtgagcgagg tggtcgaagc gctcaagacg agcagccccg cgacgctcat gatcggccgc
     1441 gacgccttct tcgggatcgc cgacctcgac ttcgatgcgg ccctcgatcg tctccagacc
     1501 gggcttaccg ccaccgccat gaccgaagac gcggcggagg gattggccgc gttccgtgag
     1561 aagcgggaac cgagatggaa gtaaagaccg ccccggtcgc caccaggtcg ggctgggcac
     1621 gcgccgtgct cggctggacg ctgatctgga gcgggctgtt catcttcggc tatctcggat
     1681 ggcagctgtt cgtcacagat ctggtcaatg cccgggtcca ggcgactgcg gtcgtggaac
     1741 tcgacgagac cctcgaggaa gccagggaag acccgccggt ggtcgaggaa gtcgaggccg
     1801 acgacggtgt ggtggtcgag ttctatcccg aggaggcccc ggccgaggac gaggagttcg
     1861 ccatgatccg ggtcccgagc ctgggtctgg aggcggtggt gttcgagggg gtgagcctgg
     1921 acaccctgag caaggggccg ggtcacatgc cgggaacccc gctgccgggc cagccgggaa
     1981 acgcggtgat ctcgggtcat cgcaccactc acggccgccc gttctacgac ttcgacctgc
     2041 tggtctcggg agacaggatc gaggtggaga ccgcgctggg ccggcacgtc tacgaggttc
     2101 gagactcgtt cgtcgtcgag ccgaccgatg tctgggtcgc cgatgaccgt tccggtggct
     2161 ggctgaccct caccacgtgc aatcccaagt tctctgcccg tgagcggctg atcatcgttg
     2221 ccgagatggt ggaaggcccc aatctggagt atgtgaacct gatcgaggat ggcgtcggcg
     2281 ggctcgccta ggggatcagc ccctgcaggc tctcttcggt caggatcccg ttgaacacct
     2341 cgcggatcac accgtcggcg tcgatgaagt aggtcaccgg gagaccgaag ttgggatagg
     2401 cggtctcgaa gtcgtctcca cccagcccca gcggatcaga aggctcgagc tcggcggcga
     2461 aggcgagtgc gtcgtcgagc gtgtcctcta ccgcgacccc gatcacagcg atctccggat
     2521 tgcgaaccgc gaagtcggag agggccggga tctcctcccg gcacggggcg caccacgacg
     2581 cccagagatt gagcaggacc ggacgcccgt cgtcctcgag gtggccctgg aggctgaacg
     2641 tcgagccgtt gaacagctcg acgctgaagt cgggcgcggc atctccttca gttgcgatgt
     2701 cgccgccacc gtccagcacc catgagagcg ccagcccgaa caccagcaca ccgatgaaga
     2761 ctccgctgag caggaaccgc cggctcggga gggggtcgac cggttcttca ggcagaggcc
     2821 atcttcctca acacgtagtg aaggatccca ccgttgcgca ggtactcgac ctcgatgggc
     2881 gtgtcgacgc gggcgatggt gtggaactcc ttccgcgaat cgtcctgagc cgtggcacga
     2941 accaacacac tctgtcgtgg ctgcagaccg tcgtcgatct cgatgtcgaa g
//
LOCUS       DATITW010000757         1936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_517389, whole genome shotgun sequence.
ACCESSION   DATITW010000757 DATITW010000000
VERSION     DATITW010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517389"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1357
                     /locus_tag="VLS86_10580"
     CDS             86..1357
                     /locus_tag="VLS86_10580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSO50982.1"
                     /translation="MNQTPFWVDDHPRPRNITSELPPQADVAIIGSGLTGLTAALRLA
                     RGGKTVVVVDSGEIASGASSINGGMVSPDIKAGIQVIFAQHGPELGRAMWQATVRSVE
                     IVSELAAAEAIDARIVRRGMAALGSNQATRAKFEASVAWYRENVGVDWEVLGEDRIGE
                     VVGGNHFTSALFEPEGMGIHPARFVFGIAERAATAGAILVEQCQARSFDRDGSGFRVH
                     TAKGVVTAGDVVLATNGYTTAEPSPELASGVVPVGSYIIVTEPLGHRAAAAIFPGGAM
                     TYTKKRLLNYMRRTPDDRILIGGRRNLHTGLDLEESAADLHRRLLGFFPDLEGVPITH
                     AWGGKLGVTFDLVPHMGQVDGAWYALGYAGHGVGLSTLMGHDLAGMLLGEEPPSVFTK
                     VPHPRRFYYRRNPWFLTPASLLYRALDRLNR"
     gene            1367..1663
                     /locus_tag="VLS86_10585"
     CDS             1367..1663
                     /locus_tag="VLS86_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014988829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HSO50983.1"
                     /translation="MSFPETPAYPESSQATTALVMGILGIVCCQPLGIVAWIMGNNEL
                     EGIRAGRRNPANEGTANAARILGMVSVALLAIGLVILALIIAGAMVLPFTSEFS"
     gene            1687..>1936
                     /locus_tag="VLS86_10590"
     CDS             1687..>1936
                     /locus_tag="VLS86_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006898744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HSO50984.1"
                     /translation="MTLAAPVLLVGFLGVLAPAEGGPTMCPFALCTGMACPGCGMTRA
                     ASNLIRGDLTTALAYHPLVPLAAAMSLAGWIWYLLRRSG"
BASE COUNT          304 a          651 c          644 g          337 t
ORIGIN      
        1 ggcttcaggt gtccccaccc ttgatcatga ccccggatca ggtcgccgag atggccaccc
       61 tgttccgtgc cggtctagaa agtctgtgaa ccaaaccccg ttctgggtcg acgatcaccc
      121 ccgaccccgg aacatcacct cagagcttcc cccccaggcc gatgtcgcga tcatcggctc
      181 cggcctgacc ggtctcaccg cggcactgcg gttggcgcgg ggcggcaaga cggtggtggt
      241 ggtcgattcg ggcgagatcg cctccggggc ttcgagcatc aacgggggca tggtctcccc
      301 cgacatcaag gccgggattc aggtcatctt cgcccagcac ggcccggagc tgggccgcgc
      361 catgtggcag gcaacggtgc gctcggtcga aatcgtctcg gaactggccg ccgccgaggc
      421 catcgacgcc cggatcgtgc ggaggggtat ggccgcgctc ggctcgaatc aggcgacgag
      481 agcgaagttc gaggccagtg tcgcgtggta tcgcgagaac gtcggcgtcg actgggaggt
      541 gctcggggag gaccggatcg gtgaggtagt cggcgggaat catttcacga gcgctctctt
      601 cgagcccgag ggcatgggga tccacccggc acgcttcgtg ttcgggatcg cggaacgggc
      661 ggcaacggcg ggggcgatcc tcgtcgagca gtgtcaggcc agatctttcg acagggacgg
      721 gagcgggttc agggtccaca ccgccaaagg cgtggtcacc gcaggtgacg tcgtcctcgc
      781 caccaacggc tacaccaccg ccgagccgtc tccggagttg gccagcgggg tggtgcccgt
      841 cggctcctac atcatcgtca ccgagccctt ggggcatcgt gccgccgccg ccatcttccc
      901 cggaggggcg atgacctaca ccaagaagag gctcctcaac tacatgaggc ggacgcctga
      961 cgaccggatc ttgatcgggg gcagacgcaa cctgcacacc ggtctcgacc tcgaagagtc
     1021 cgcggccgat ctccatcgcc ggcttctcgg gttctttccc gacctggagg gggtcccgat
     1081 cacccatgcg tggggcggca agctcggcgt caccttcgat ctcgtccctc acatgggcca
     1141 agtcgacggc gcctggtacg ccctcggata cgccgggcac ggggtgggtc tgtcgacgct
     1201 catgggccac gatctggccg gcatgctgct cggtgaggag ccgccgagtg tgttcaccaa
     1261 ggtcccgcac ccgagacggt tctactaccg gcgcaatcca tggtttctca ctccggcgtc
     1321 gctcctatac cgggcactgg atcgtctcaa ccgataggct cgggccatga gcttccccga
     1381 aacccccgca tacccggagt ccagccaggc gacgaccgca ttggtcatgg gaatcctcgg
     1441 aatcgtctgc tgtcagccgc tcgggatcgt ggcctggatc atgggcaaca acgagttgga
     1501 ggggatccgc gccgggcgga ggaacccggc caacgagggg acggccaacg cggcgaggat
     1561 cctgggcatg gtcagcgtgg cgctgctcgc aatcgggttg gtgattctcg ccctcatcat
     1621 cgccggggcc atggtgctcc cgttcaccag cgagttcagt tgatgaccca gacgtggcgg
     1681 caccgcttga ctctcgccgc cccggtgctc ctcgtcggct tcctcggggt gctggccccg
     1741 gcggagggcg gtccgaccat gtgcccattc gccctgtgca ccgggatggc gtgccccgga
     1801 tgtggcatga cccgtgccgc ctcgaacctg atcagaggcg atctgaccac cgctctcgcc
     1861 taccacccgt tggttccact ggccgcggcc atgtcgctcg ccgggtggat ctggtacctg
     1921 cttcgccgat ccggtc
//
LOCUS       DATITW010000758         1702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_397310, whole genome shotgun sequence.
ACCESSION   DATITW010000758 DATITW010000000
VERSION     DATITW010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397310"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VLS86_10595"
     CDS             <1..226
                     /locus_tag="VLS86_10595"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HSO50985.1"
                     /translation="YLGMFTRFRVDVGGQLLEIATQNKGVDEENPSVQVGQSVRVTWL
                     KSSCRPLDESPQADDAGEAAPALAGVETTP"
     gene            257..1429
                     /locus_tag="VLS86_10600"
     CDS             257..1429
                     /locus_tag="VLS86_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013654575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSO50986.1"
                     /translation="MLLLTVFALFLTACGDDAGDEEGEDLPTEIGDGEGSVVIVAWAG
                     YIERGDTDPAYDWVTGFEEETGCMVEVKQANTSDEMVALMTASEEYDLVTASGDASLR
                     LIAGGTVQEVNVDLVPSWDTVDDRLQDAPWHTVDDKHYGVPYQWGANVLAYNTEVFGD
                     TTPDSWSVVFEETELPDGATNAQRVQAYDGPIYVADAALYLKSAQPELGIEDPYQLND
                     EQYAAALDLLRQQRALVGRYWHDAFVQIDDFTNEGVAASSSWPFQVNILQGSEAPIAS
                     VIPAEGATGWADTTMMHANAPHPNCAYMWLEWSISPAVQGDLAAWFGSVPAVPSACEG
                     NDLLGPDGCVTNGIDNFDQISFWKTPTEDCNGTAEAGSCVPYYRWVTDYLAVIGGR"
     gene            1479..>1702
                     /locus_tag="VLS86_10605"
     CDS             1479..>1702
                     /locus_tag="VLS86_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO50987.1"
                     /translation="MTTDTKPRPILHRAATGLYRRRWLRLALLLALPVLWIVGVYGGS
                     LIALLTQSFYALNPFTGTVDDQLTLDTWRTL"
BASE COUNT          315 a          548 c          556 g          283 t
ORIGIN      
        1 ctatctcggg atgttcaccc gcttccgggt cgacgtcggt ggtcagctgc tcgagatcgc
       61 gacccagaac aagggagtcg atgaggagaa tccgagcgtt caggtgggac agagtgtgcg
      121 tgtcacctgg ctcaagagtt cgtgtcggcc gctggacgag agtccccaag cggacgacgc
      181 gggcgaggca gccccggccc tggccggggt ggaaaccacc ccttagagga ggaatagaca
      241 tatgagatct cgtttggtgt tgctcttgac cgtcttcgcc ctgtttctca cggcatgtgg
      301 cgacgacgcc ggcgatgagg agggcgagga tctgcccacc gagatcgggg acggcgaagg
      361 cagcgtcgtg atcgtcgcct gggccgggta tatcgagcgc ggggacacag acccggcgta
      421 tgactgggtg acggggttcg aggaggagac aggctgcatg gtcgaggtga agcaggccaa
      481 cacctccgat gagatggtcg ccctcatgac cgcgtccgag gagtacgacc tggtcacggc
      541 ctccggcgat gccagtctcc gcctgatcgc aggcgggacc gttcaggagg tgaatgtcga
      601 cctggtgccg agttgggaca cggtggacga tcggctccag gacgcgccct ggcacacggt
      661 ggacgacaag cactatggag ttccctatca gtggggcgcc aacgtgctcg cctacaacac
      721 cgaggtgttc ggcgacacga cacccgactc atggagcgtc gtcttcgagg agacggagct
      781 ccccgacggt gccaccaatg cccagcgggt gcaggcctat gacggcccga tctacgtggc
      841 cgacgcggcc ctctatctga agagcgcaca acccgagctc ggcatcgagg acccatacca
      901 gctcaacgac gagcagtacg ccgccgccct ggatctgctg cgacagcagc gagccctggt
      961 cggtcgctac tggcacgacg ccttcgtgca gatcgacgac ttcaccaacg agggagtggc
     1021 ggcatcgtcg agctggccgt tccaggtcaa catcctccag ggatccgaag cgcccatcgc
     1081 ctcggtgatc cctgcagagg gagccaccgg atgggccgac accacgatga tgcatgccaa
     1141 tgccccccac cccaactgcg cctacatgtg gttggagtgg tcgatcagcc cggctgttca
     1201 aggcgacctg gccgcgtggt tcggatcggt cccggccgtc ccctccgcat gtgagggcaa
     1261 cgacctcctc ggccccgatg gctgtgtcac caacgggatc gacaacttcg accagatttc
     1321 gttctggaag accccgaccg aagactgcaa cgggacggcg gaggccggat cttgcgttcc
     1381 ctactaccgc tgggtgaccg actacctggc tgtgatcggc ggacgctgat cgaatcgagt
     1441 gggtggggcg gcctcggccg ccccacccac cagacgtgat gacaacggac accaagccaa
     1501 gaccgatcct gcatcgcgcc gccaccgggc tctaccggcg tcgctggctg cggctcgcgc
     1561 tcctgctcgc cctcccggtg ctctggatcg tcggggtcta cggcggaagt ctgatcgcgc
     1621 tcctgacgca gagcttctat gccctaaacc ctttcaccgg caccgtcgac gaccagctga
     1681 cgctcgacac atggaggacg ct
//
LOCUS       DATITW010000759         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_37317, whole genome shotgun sequence.
ACCESSION   DATITW010000759 DATITW010000000
VERSION     DATITW010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37317"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /gene="purN"
                     /locus_tag="VLS86_10610"
     CDS             complement(<1..544)
                     /gene="purN"
                     /locus_tag="VLS86_10610"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HSO50988.1"
                     /translation="MSGNTLPPRSDGRGEVTIRVAVLASGSGTNLQALIDTADLGAEI
                     AVVISDRADATALRRAESAGIATRVIGRDGFESREGFSEAIGVEIEAAGARGVVLAGF
                     MRILAPNLIGRFPNRILNIHPSLLPSFPGANAVARALEHGVKVTGVTVHFVEVEVDQG
                     PIIAQRAVPVLPDDDLTSLHA"
     gene            596..892
                     /locus_tag="VLS86_10615"
     CDS             596..892
                     /locus_tag="VLS86_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSO50989.1"
                     /translation="MIENVPAEKWETWVEETGALILDIREPFEWTQGTLPGATLIPMG
                     EIPTRMVELDTEQALLVVCRSGGRSHQVAMYLTANGYARAANMAGGMKALGLQD"
     gene            1033..1476
                     /locus_tag="VLS86_10620"
     CDS             1033..1476
                     /locus_tag="VLS86_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO50990.1"
                     /translation="MEDESRLPGWMVPAVGVAVVVVLVIIGLSRGPATLDPSTPEGTV
                     QLYIQSVVEGDFDTASTYWADDGCTPTSEIPTGGTPDVSASLVSVDGNDIEASVTVRL
                     TESSPDPLGGLYEYEEWFTLIRNGETWKIRQPAWPYWDMVCEESA"
     gene            1473..>2626
                     /locus_tag="VLS86_10625"
     CDS             1473..>2626
                     /locus_tag="VLS86_10625"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HSO50991.1"
                     /translation="MTAALGGLVLLAIVGGLIAFGVKAWRGRNEQEDHGGLDLIPYLI
                     LALAVGIAGFSLSRLARASLTPDRFAGRPTGEIAAALAGLVVAAPVAYFLWRRQVGRR
                     KTYPHNPGWPIYLSVIEVVFLTAFYVSVAQIADALTSTAITATWPDLLVYGGIVAFHW
                     WAERREPPYGDAGELPRLIGSGVSGIALTVGAIGTLTWLLSEAYESIGPTITIPEPAV
                     PLALTLVGAPIWAWRWLPAWRDETSVFRSLYLAAVTALSLAVAVGAAVATTAVLLSYL
                     LGQAGPAADHFSSYPPAIAFGIVGGALWIHHRRRLGPGRTGALRGYQYAMAAAGLSAL
                     IGATVALIELVFSQRLAGDATGEGLIALGCAAIAAGWVWLWFWRKVQAAPR"
BASE COUNT          411 a          839 c          916 g          460 t
ORIGIN      
        1 gggcatggag cgatgtgagg tcgtcatcgg gcaggacggg gacggctcgt tgggcgatga
       61 tcggtccctg gtccacttct acttccacga aatggacggt aaccccggtc accttcactc
      121 cgtgctccag ggcgcgagcg acagcattgg cccccgggaa cgacgggagg agggaggggt
      181 ggatgttcag tatccggttg gggaaacggc cgatcagatt cggggcgagg atgcgcatga
      241 agccggcgag aaccacacct cgtgccccgg cagcctcgat ctcgaccccg atcgcctccg
      301 agaagccctc tcgcgactcg aagccgtcgc ggccgatgac acgggtcgcg atcccggcgc
      361 tctcggcccg ccgcagagct gtggcgtctg cgcggtcgga gatcaccact gcgatctcgg
      421 cgcccagatc ggcggtgtcg atgagggcct ggagattggt cccggacccc gaggcgagga
      481 ctgcgactct gattgtgacc tcccctcgac cgtccgatcg cggcggcagt gtattccccg
      541 acatcacggc caatcggctt gaatcagcgg tgaacatgac tgagcgggtg gattcatgat
      601 cgagaacgtc ccggcggaga aatgggagac ctgggtcgag gagaccgggg cgctcatcct
      661 cgacatccgc gagccgttcg agtggacgca ggggaccctt cccggcgcga ccttgatccc
      721 catgggggag atcccgacga gaatggtcga gctcgacacc gagcaggcgc tcctcgtggt
      781 gtgccgatcc ggcggcagaa gtcaccaggt ggcgatgtac ctcaccgcca acgggtatgc
      841 cagggccgcc aacatggccg gtgggatgaa ggccctcggc ctccaggact gacgcctcgc
      901 cctcgccccc ctcaccgtaa ttgggagccc caggtccgta tttgacccct ggcggtccca
      961 attacggggg tggggatggc ggaaccggct ccgggcaccg agaccgtgcc cggctctgcg
     1021 agaatgagcg ccatggaaga tgagagcagg cttccgggat ggatggtccc ggccgtcggt
     1081 gtggccgtgg tggtcgtgct ggtgatcatc ggtctctccc gcggccctgc cacgctcgat
     1141 ccgtcgaccc cggagggaac ggttcagctc tacatccagt cggtggtcga gggtgacttc
     1201 gacaccgcct ccacatactg ggcggatgac ggctgcacgc cgacatcgga gatcccgacc
     1261 gggggaacac ccgacgtctc cgcatcactg gtgagcgtcg acggcaacga catcgaagcg
     1321 tcggtcaccg tgaggctcac cgagagctcc cccgacccgc tcgggggtct ctacgagtac
     1381 gaggagtggt tcacgttgat cagaaacggc gaaacgtgga agatccgtca gccggcctgg
     1441 ccctactggg acatggtctg tgaggaatcg gcgtgacggc ggcactgggc ggcctggtgc
     1501 tcctcgccat cgtgggcggt ttgatcgcgt tcggggtgaa agcgtggcgt ggtcgcaacg
     1561 agcaggagga ccatggcggg ctcgatctga tcccgtatct gatccttgcg ctggcggttg
     1621 gcatcgccgg gttctctcta tcccgtctcg ccagggcgag tctcactccc gaccgcttcg
     1681 ccggacgacc gaccggtgag atagcggcag cgctggccgg tctggtggtg gcggccccgg
     1741 tcgcctattt cctgtggcga cgccaggttg ggcggaggaa gacctacccc cacaacccgg
     1801 gatggccgat ctacctcagc gtgatcgagg tggtgtttct caccgctttc tacgtctcgg
     1861 tggcccagat cgcagatgcc ctgaccagca ccgcgatcac cgcgacctgg ccggatctcc
     1921 tggtgtatgg gggaatcgtg gccttccatt ggtgggccga gagacgcgag ccgccctatg
     1981 gcgacgcggg cgagctaccc cgcctgatcg gatcaggggt gtcggggatt gccttgaccg
     2041 tgggagccat cggcacgctc acctggctgt tgtccgaggc gtatgagagc atcgggccga
     2101 cgatcaccat ccccgagccg gccgtgccac tcgccctcac cctggtcggc gccccgatct
     2161 gggcctggag atggctgccc gcatggcggg acgaaaccag cgtgttccgc agtctctatt
     2221 tggctgcggt gacggcgctg tcgctggcgg tggctgtcgg tgccgcagtg gcgacgaccg
     2281 ctgtgctcct ctcctacctg ctggggcagg ccggtccggc agcggaccac ttcagcagct
     2341 atccgccggc gattgcgttc gggatcgtcg gtggcgcgct atggatccac caccgccgtc
     2401 gtctcggccc gggacggacc ggagcactcc gcggctacca gtacgccatg gctgcagcag
     2461 gcttgagtgc gctcatcggg gcgacggtgg ccctgatcga actcgtcttc tcgcagcggc
     2521 tggccgggga cgccaccggt gagggtctca tcgccttggg ctgtgctgcg atcgcggcgg
     2581 gatgggtatg gctctggttc tggcgcaagg tccaggcggc gccccg
//
LOCUS       DATITW010000760         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_196930, whole genome shotgun sequence.
ACCESSION   DATITW010000760 DATITW010000000
VERSION     DATITW010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196930"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="VLS86_10630"
     CDS             complement(<1..1583)
                     /locus_tag="VLS86_10630"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HSO50992.1"
                     /translation="MIPVHYDLQLAPDLDSFTFEGSVSIQIGITETLDEIILNAAEID
                     MKSVVLRGEDLEIPVTGIAHDDEYERATLTLETPANPGTYVLDISYTGQINDQLRGLY
                     RSLYRDADGTEHVIATSQCQSTEARRVFPCWDEPDLKATFKTTMVVAEGLEAYSNGAE
                     LARTPLEDGRVRFEFSPTMKMSTYLLAFIVGPFEATEPAMVRGTPIRVIVPKGNLHLT
                     DVALENAVFCFEYLSDYYGIPYPGDKLDHIAIPDFAAGAMENVGLITYRDAYLILDRA
                     KASQGELQGCLDVIGHEIAHQWFGNLVTMKWWEGAWLNEAFASFMELKATDAKKPEWK
                     RWLAFNNLEIPWAMGTDQLVTTRPIEFEVNSPSEVDEMFDAITYGKGSAVLRMIEQFI
                     GEEEFRTGVGNYLRKHEYANTVTSDLWEGLDGASDWPVGEIMNTWVYQRGFPQIDVKV
                     AGDGIQLAQHRYLAIPDETDATIWDVPIQLRGIVDGRPFEKKVLLTEDEMVIPLDASV
                     DYMVANAGGHGFYRTRYSE"
     gene            1723..>2811
                     /gene="serB"
                     /locus_tag="VLS86_10635"
     CDS             1723..>2811
                     /gene="serB"
                     /locus_tag="VLS86_10635"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HSO50993.1"
                     /translation="MSEHSTILVGVHGPDAPGISAGLMKLLAEADVEIYDIEQIVVRG
                     RLTLNVLIGVSGERATVRDLLFFGWERGLHVEFEVVEDKPTPGRPMSVVTVIGMRVGP
                     EDFGAVASAIAEGGGNIDRIFRLSRYPVVSYELLVGNGDLDRIRANLVLAAAERPIDV
                     AFQREGLERRAKRMVVMDVDSTLIQNEVINLLAREAGVEDRVSELTQLALTGEVDFEA
                     ALRERVGMLKGLDQTAITRVHENITLTSGARTFIRTLKRLGLVTAIVSAGFTRFTDVL
                     AADLGIDYSLSNTLEMVDGVLTGELVGEFVDGPKKAAFLRTIAEAEGIPLSQVVAVGD
                     GANDLEMLATAGLGIAFNAKPIVSERADT"
BASE COUNT          474 a          869 c          935 g          533 t
ORIGIN      
        1 tcttcggagt agcgggttcg gtagaagccg tgcccgccgg cgttggccac catgtagtcg
       61 acgctggcgt cgagcgggat caccatctcg tcctcggtga gcagcacctt tttctcgaac
      121 ggccggccgt cgacgatgcc tctgagctgg atcggcacat cccagatcgt ggcgtcggtc
      181 tcgtcgggga tggccagata gcgatgctgg gcgagttgga tgccgtcgcc tgccaccttg
      241 acgtcgatct gggggaatcc acgctggtac acccaggtgt tcatgatctc gcccacgggc
      301 cagtccgacg ctccgtcgag accctcccag agatcactgg tgacggtgtt ggcgtactcg
      361 tgcttgcgca ggtagttgcc caccccggtg cggaactctt cctcgccgat gaactgctcg
      421 atcatgcgga ggacggctga gcccttgcca taggtgatgg catcgaacat ctcgtcgact
      481 tcgctgggcg agttgacctc gaactcgatg ggtcgtgtcg tcaccagctg atcggtgccc
      541 atcgcccacg ggatctccag gttgttgaac gccagccagc gcttccactc gggtttcttg
      601 gcatcggtcg ccttgagctc catgaagctg gcgaatgcct cgttgagcca ggctccctcc
      661 caccatttca tggtcaccag attgccgaac cactggtggg cgatctcatg gccgatcaca
      721 tcgagacacc cctggagctc gccctggctg gccttggccc ggtccaggat gagataggca
      781 tcccgatagg tgatcaggcc cacgttctcc atcgccccgg cggcgaagtc agggatcgca
      841 atgtggtcca gcttgtcgcc cggataggga atcccgtagt agtcggagag atactcgaag
      901 cagaacaccg cgttctccag cgccacgtcg gtgagatgca ggttgccttt gggcacgatc
      961 acccgaatcg gcgtcccccg caccatggcc ggctcggtcg cctcgaacgg gccgacgatg
     1021 aaggcgagga ggtaggtcga catcttcatc gtcgggctga actcgaacct caccctcccg
     1081 tcttcgagcg gggtgcgcgc cagctcggcg ccgttggagt aggcctcgag accttcggcc
     1141 accaccatcg tggtcttgaa cgtcgccttg aggtcaggct cgtcccagca gggaaagacc
     1201 cggcgggcct cggtcgactg gcactgggac gtggcgatca cgtgctcggt gccgtccgcg
     1261 tcgcggtaga gcgagcggta caggccgcga agctggtcgt tgatctgacc ggtgtaggag
     1321 atgtccagga cgtaggtgcc cgggttggcc ggcgtctcca gggtcagggt cgccctctcg
     1381 tactcgtcgt cgtgagcgat gccggtcaca ggtatctcga ggtcctcgcc gcggagcacg
     1441 accgacttca tgtcgatctc tgcggcgttg aggatgatct catcgagggt ctcggtgatg
     1501 ccgatctgga tcgagaccga tccctcgaag gtgaagctgt ccaggtcggg cgcaagctgc
     1561 aggtcgtaat ggaccggaat cacggtccga ggcaggcgga aagggttgtc agtccataga
     1621 gcctcttggt cgtaggtgac ctgcagatgg taccgacaac ccctcaatcc cccaatcgcg
     1681 gcctgacatt gaacacgatg gcgaactgct taccctgtac atgtgagcga gcactcgacg
     1741 atcctggttg gggtccatgg ccctgacgcc cctggcatct ccgccggcct gatgaagctc
     1801 ctcgccgagg cggatgtcga gatctacgac atagagcaga tcgtcgtccg tggccgcttg
     1861 acgttgaacg tcctgatcgg ggtctccggg gagcgggcca cagttcgcga cctgctcttc
     1921 ttcggttggg agcgtggcct tcatgtcgag ttcgaggtgg tcgaggacaa gccgacaccg
     1981 gggcgcccga tgtccgtggt cactgtcatc gggatgagag tgggcccgga ggacttcggc
     2041 gccgtggcgt cggccatcgc cgaaggggga gggaacatcg accgcatctt ccgtctctcc
     2101 cgctatccgg tggtcagcta cgagttgctg gtcggcaacg gggatctgga tcggatccgg
     2161 gccaacctgg tgctcgccgc cgcggagcga ccgatcgacg tcgccttcca gagggagggt
     2221 ctggagcgca gggcgaagcg gatggtggtc atggacgtcg attccaccct gatccagaac
     2281 gaggtgatca acctgctggc gcgcgaggcg ggagtcgagg accgggtctc ggagctgacc
     2341 caactggcgc tgaccggcga ggtcgacttc gaggcggcgc ttcgggagcg ggtgggcatg
     2401 ctcaaggggc tggatcagac cgcgatcacc cgggtccacg agaacatcac cctcaccagc
     2461 ggggctcgca ccttcatccg gaccctgaag cgtctcggtc tggtgacggc gatcgtctcc
     2521 gcaggcttca cccgattcac cgatgtcctc gccgccgacc tgggaatcga ctactcgctg
     2581 tccaacactc tggagatggt cgacggcgtg ctcaccgggg agctggtcgg cgagttcgtc
     2641 gacgggccga agaaggcggc attcctgcgc acgatcgccg aggccgaggg gatccccctg
     2701 tcacaggtgg tggcggtcgg cgacggcgcc aacgacttgg agatgctggc cacagcaggc
     2761 ctgggcatcg cgttcaacgc caagccgatc gtcagtgagc gggccgacac c
//
LOCUS       DATITW010000761         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_718421, whole genome shotgun sequence.
ACCESSION   DATITW010000761 DATITW010000000
VERSION     DATITW010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718421"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VLS86_10640"
     CDS             complement(<1..488)
                     /locus_tag="VLS86_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018516015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HSO50994.1"
                     /translation="MDLNRLTEKSQQALSEAQSSAIRLGHTEVDGEHLLLALLTQPEG
                     LVPRLVTQAGGDPARLVDELESDLAQRPRVSGSGLSPGEIRVTRRLAQLLEAAEREAR
                     QLKDEYVSVEHLVIAFLGERDDTAAKRSLTSQGITRDGFLGALTAVRGNQRVTSATPE
                     AA"
     gene            712..1365
                     /locus_tag="VLS86_10645"
     CDS             712..1365
                     /locus_tag="VLS86_10645"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HSO50995.1"
                     /translation="MNWGRLYFGSLIVAVGTLLLLDNADILDAGDVIGDWWPAALIVA
                     GLLTFAANPRHWPVALVITAVGVAFLLSNLGIVDIGDIIIPAAIILVGILVILGRGLG
                     SRTEAGDTVNSFNVFSGSEVASHSKQFRGGSITAVFGGAEVDLRDAVPAADAQLDVFA
                     AFGGVEIRVPDGWHVITRGLPLFGGIENVTAREQLPEDAPVLPISATALFGGVEIKH"
     gene            complement(1362..1808)
                     /locus_tag="VLS86_10650"
     CDS             complement(1362..1808)
                     /locus_tag="VLS86_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HSO50996.1"
                     /translation="MDVAPQPRTRHPWGDAPTPYEALGGDDVVRALVECFYDLIEAES
                     PVLRAMLPRDTSTSRQKLYVFLSGWLGGTPLYMERRGHPRLRLRHMPFDIGSPEAEEW
                     MRCMKKAIDVVGIEDPMASFLSGELAKTAAGLRNRPDPGVIFPRGT"
     gene            1938..>2771
                     /locus_tag="VLS86_10655"
     CDS             1938..>2771
                     /locus_tag="VLS86_10655"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HSO50997.1"
                     /translation="MDRVSARVLNRTTLHRQSLLDRSDSTAAAMIEHLVGMQAQNPND
                     PYFALWARLTSFDQSELSTMIREGLAVRGQLMRATIHLVTTRDFLALRPQLQSVCART
                     LGSTSFARDTAAVDRSALLNEGRALLEERPMTRAQLGGALEPRWPGVPPASLAQVVTY
                     LLPAVQVPPRGMWQRSAPAAWTTIEGLTGGELTDQPSVEDTVLRYLAAFGPASIKDVR
                     AWSGLPGLKEIVDRIRPRLRTFTGESGVELLDLPQATLVEPDVPAPPRFLPEYDNVLL
                     GH"
BASE COUNT          460 a          930 c          899 g          482 t
ORIGIN      
        1 taagccgcct ccggcgtcgc agaggtcacg cgctgattgc ctcgcaccgc ggtgagcgca
       61 ccgagaaagc catctcgtgt gatcccctga gatgtcaggc ttcgcttcgc ggccgtgtca
      121 tcccgctccc ccaggaaggc gatcaccaga tgctccaccg agacgtactc gtccttgagc
      181 tgtctggcct ccctctccgc cgcctcgagt agctgagcca gacgtcgtgt gaccctgatc
      241 tcgcccggcg acagacccga gccgctcacc ctcgggcgct gcgccaggtc ggactccagc
      301 tcgtcgacca gtcgagcagg atcgccaccg gcctgggtaa ccagacgtgg cacgagaccc
      361 tcgggctgcg tcaacaatgc aagcagcaga tgctcgccgt cgacctcggt gtggccgagg
      421 cgaatcgccg atgactgcgc ttcgctcagg gcttgctgtg atttctcggt gagacggttc
      481 aggtccatag tccgctacca ggtccacgat cgcggtgccc gatcctacgc cgaggatggt
      541 ccaaagttcg ggcgcgccgg cgaccgacat cgtgcggatt ttgccgggag agggcgattc
      601 gaacgacaga gacagctgcc gtcctccccg ggtgggagcc gaatcgttgg acgtggccga
      661 ccgccctccg aaccggtgag ccgatagagt cagccgccca aaggaggctc tatgaactgg
      721 ggacggttgt acttcgggtc cctgatcgtg gcggtgggca cactgttgct tctggacaac
      781 gccgacatcc tcgacgccgg tgacgtgatc ggcgactggt ggccggcggc cttgatcgtg
      841 gccgggctcc tcaccttcgc ggccaaccca cgccattggc cggtggccct ggtcatcacc
      901 gcggtcgggg tcgcgtttct gctctccaac ctcggcatcg tggacatcgg cgacatcatc
      961 atcccggctg cgatcatcct cgtcggcatc ctggtcatcc tcgggcgggg gctcgggtcg
     1021 aggaccgaag ccggggacac ggtcaacagc ttcaacgtct tttccggaag cgaggtcgcc
     1081 tcccattcca agcagttccg cggggggagc atcaccgcgg tgttcggcgg ggccgaagtg
     1141 gacttgaggg atgcagtccc tgcggcggac gcccaactcg acgtctttgc cgcctttggc
     1201 ggcgtcgaga tcagggtccc cgacggatgg catgtcatca ccaggggtct gccccttttc
     1261 ggcggcatcg agaacgtgac cgccagagag caattgcccg aggatgctcc ggtcctaccc
     1321 atcagtgcca ccgcgctgtt cggaggggtc gagatcaagc actaggtccc gcgggggaag
     1381 atcaccccgg gatcgggccg attgcgaagc ccggccgccg tctttgccag ctcgcccgag
     1441 agaaacgaag ccatcgggtc ctcgatgccg acgacatcga tcgccttctt catgcaccgc
     1501 atccactcct cggcctcggg actgccgatg tcgaaaggca tgtggcgaag ccggagtcgg
     1561 ggatggcccc gacgctccat gtagagcggg gtgcctccca gccatccgct gaggaacacg
     1621 tacagcttct ggcgggaggt ggaggtatct ctggggagca tcgctcgcag aacgggtgac
     1681 tcggcctcga tcaggtcgta gaagcactcg accagggccc ggaccacatc atcgccacca
     1741 agcgcctcat atggcgtcgg ggcgtctccc caggggtgcc tggttctggg ctggggtgcg
     1801 acatccatgg ccggcccacg gtaccgagac ccgtgagaaa gccaatgggc agggatcggg
     1861 gcggatagtc gtgctccagg cgtcgggagg ggctcgacga cccgtggcat ggcgtcctta
     1921 gagtccgggg ccaagtcatg gacagagtca gcgcccgagt cctgaaccga acaacccttc
     1981 accgtcaatc tctgctcgat cgatccgact ccaccgcggc ggccatgatc gagcatctgg
     2041 tggggatgca ggcacagaat cccaacgacc cctacttcgc cctttgggca cgactgacga
     2101 gcttcgacca gtcagagctc tccaccatga tcagagaagg gctggcggtg cggggccagc
     2161 tgatgcgggc cacgattcac ctggtcacga cacgagattt cctggccctc cgaccccagc
     2221 tccagtcggt gtgtgcccgg accctgggct cgacatcgtt tgccagagac accgcggcgg
     2281 tcgaccggtc ggcgctgttg aacgagggca gggcgctgct cgaggaacga ccgatgaccc
     2341 gcgcccagtt gggcggtgct ctcgagcccc gatggccggg ggttccgcct gcctccctcg
     2401 cccaggtggt cacctatctg ctgcccgcgg tgcaggtgcc accgcgcggg atgtggcaga
     2461 ggagcgctcc tgccgcctgg acgaccatcg agggcctcac cgggggggag ctcaccgacc
     2521 agccgtcggt cgaggacacg gtcctgcgtt atctcgccgc atttggcccg gcctcgatca
     2581 aggatgtgcg ggcgtggtcc ggtctccccg ggctcaagga gatcgtggat cgaatcaggc
     2641 cccggcttcg caccttcacc ggcgagagcg gcgtcgagct tctagatctg ccccaggcca
     2701 ccctcgtcga gcccgatgtc cctgccccgc cccgattcct tcccgaatac gacaacgtcc
     2761 tgctgggtca c
//
LOCUS       DATITW010000762         1854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_517517, whole genome shotgun sequence.
ACCESSION   DATITW010000762 DATITW010000000
VERSION     DATITW010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517517"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VLS86_10660"
     CDS             <1..639
                     /locus_tag="VLS86_10660"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HSO50998.1"
                     /translation="GDLAAVPAQALGASVGSGSAGALHALSWNRDPRRVHGGRRAGSV
                     GSQQALGRGLTDPGELSVVVLGLADRVGRRLRKKQRSGSTVSLRVRFPGPRLVSRSHT
                     LPTPTSSTAALSELGGVLLGRAIQGQHEPVTLVGLSVSNLTTDDFLQLELDIDPGDVL
                     RAGSGLDLKRRALDASVDGVRERFGRKLLKLGAAPERGVASEEFRRLAEHSD"
     gene            complement(631..1197)
                     /locus_tag="VLS86_10665"
     CDS             complement(631..1197)
                     /locus_tag="VLS86_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HSO50999.1"
                     /translation="MTWLAEKWLKLRGWRFVGDMPDLDQMVLIGAPHTTNWDFIVYLG
                     AIRHWRISPRYIAKHTLFRWPFGAFFRRFGGIPVDRTRGGGLVGQVTAAFRESERMIL
                     VMAPEGTRSAAPYWKSGFVKIAADAGVPIVPVYINFPRKEVVVGSPIHFTGDEKQLMD
                     ELRVFFQAGVGKHPEGAGPVRLKEERSV"
     gene            complement(1238..>1854)
                     /locus_tag="VLS86_10670"
     CDS             complement(1238..>1854)
                     /locus_tag="VLS86_10670"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HSO51000.1"
                     /translation="GATVALIELAFSQRLAGDATGEGLIALGCAAIASGWVWLWFWRK
                     VQAAPREDEIRVLQRRIYLIGMTVVTGLTAASALIGALVVVFRAVLGETEDMIDSLRL
                     PLSLAVVSGLAAWHLFTLLRADSVGLRQVEVPPFTVTVICSHPGNITTLFPREATTRV
                     LYRADATGIVSDEMAAAIVAEVGSSSSLVWVDDTGYKIAPARES"
BASE COUNT          300 a          632 c          630 g          292 t
ORIGIN      
        1 ggcgacctgg ccgcggtccc cgcccaggcg ctgggcgcat ctgtcggctc cggctcggcc
       61 ggggcgcttc acgccttgtc ctggaacaga gacccacgcc gggtccatgg tggccggcgc
      121 gccgggagcg tcggcagtca gcaggccttg ggtcgcgggc tcaccgaccc cggggagctg
      181 tcggtggttg tgctgggtct tgccgaccgg gtcggacgca ggctgcgcaa gaagcagcgt
      241 tcggggtcca cggtctcgtt gcgggtcagg ttccccggcc cgcgtctcgt ttccaggagc
      301 cacaccctgc ccacgcccac ctcgtcgaca gccgcattgt ccgagctggg cggggtgctc
      361 ctgggccggg cgatccaggg tcagcacgag ccggtgaccc tcgtcggcct ctcggtgtcg
      421 aatctgacca ccgatgactt cctccagctc gagctcgaca tcgatcccgg cgacgtcctc
      481 cgcgccggga gtgggctcga cctcaagcgg cgcgccctcg acgcctccgt cgacggggtc
      541 agagagcggt tcggcagaaa gctgttgaaa ctgggagcgg ccccggaacg gggggtggcc
      601 tctgaggagt tccgccggct ggcggagcac tcagactgac cgctcctcct tgagtcggac
      661 cgggccggcg ccctcggggt gcttgcccac cccggcctgg aagaagacac gaagctcgtc
      721 catcagctgc ttttcgtcgc cggtgaaatg gatcggtgac ccgacgacca cttcctttct
      781 cgggaagttg atgtagacgg ggacgatcgg caccccggcg tcggctgcga tcttgacgaa
      841 acccgatttc cagtacggtg ctgcgctgcg ggtcccttcc ggggccatga ccaagatcat
      901 ccgctccgat tcccggaacg cggccgtcac ctggcccacc agaccgccgc ctcgggtcct
      961 gtcgaccggg atcccgccaa aacgtctgaa gaaggcgcca aagggccagc ggaagagggt
     1021 gtgcttggcg atgtatcggg gcgagatccg ccagtgcctg atcgcgccca ggtagacgat
     1081 gaagtcccag ttcgtggtgt gcggggcccc gatcaggacc atctggtcca ggtccggcat
     1141 gtcaccgacg aaccgccatc ccctgagctt gagccacttc tcggcaagcc aggtcacgcg
     1201 gcatcacccg tgagacatgc cgtggtccgg ggaagggtca ggattccctc gccggtgcga
     1261 tcttgtagcc ggtgtcgtcc acccagacca gcgacgagct gctcccgacc tcggcgacga
     1321 tcgccgcagc catctcgtcg ctgacgatgc ccgtggcatc ggctcgatac aggacgcggg
     1381 tcgtggcctc ccgggggaag agtgttgtga tgttgccggg atgggagcag atcacggtca
     1441 ccgtgaacgg agggacctcg acctgccgca gaccgacgct gtccgcccgg agcagggtga
     1501 agagatgcca ggcggccaaa ccggacacca cggcgaggct gagcggcagg cgcagggaat
     1561 cgatcatgtc ctccgtctcg cccaacaccg cccgaaacac gacgaccagg gcgccgatca
     1621 gggcgctggc ggcagtgagt ccggtgacga cggtcatccc gatcaggtag atccttcgct
     1681 gcaggacgcg gatctcgtct tcgcggggcg ccgcctggac cttgcgccag aaccagagcc
     1741 atacccatcc cgacgcgatc gcagcacagc ccagggcgat gagaccctca ccggtggcgt
     1801 cgcccgccag ccgctgggag aaggcgagtt cgatcagtgc caccgtcgcc ccga
//
LOCUS       DATITW010000763         1659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_758165, whole genome shotgun sequence.
ACCESSION   DATITW010000763 DATITW010000000
VERSION     DATITW010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758165"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VLS86_10675"
     CDS             complement(<1..390)
                     /locus_tag="VLS86_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019813993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSO51001.1"
                     /translation="MTQVLDGVLDLARSGLEDAIDLRRRLHRRPELGLELPATQATVL
                     EALDGLPLSVTTGTTTTSVVAVLDGDEPGPTVLLRGAMDALPVPEDTGLDYASTVDGA
                     MHACGHDAHVAMLAGAAHLLAARRGDLS"
     gene            complement(418..1605)
                     /gene="tuf"
                     /locus_tag="VLS86_10680"
     CDS             complement(418..1605)
                     /gene="tuf"
                     /locus_tag="VLS86_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HSO51002.1"
                     /translation="MGKAKFARTKPHVNVGTMGHIDHGKTTLTAAITKVLAEANPKVQ
                     FTAFDQIDKAPEEKQRGITINIAHVEYETDKRHYAHVDMPGHADYIKNMITGAAQVDG
                     AILVVSAADGPMPQTREHVLLARQVGVPSIVVALNKVDMVDDEELLELVELEVRELLS
                     EYEFPGDDTPVVRVSGLKALDGEPEAAAQVLELMAACDSFIPEPVRELDKPFLMPIED
                     VFSITGRGTVVTGKVEQGVIHTGDTVEIVGIRPTQSTTCTGVEMFRKLLDEGQAGDNI
                     GALLRGTKKEEVERGQVLCKPGSITPHTEFEGQVYVLTKEEGGRHKPFFANYRPQFYF
                     RTTDVTGTVELPEGTEMCMPGDNTLMKVTLIHPIAMDEGLRFAIREGGRTVGAGRVTK
                     IIK"
BASE COUNT          261 a          588 c          507 g          303 t
ORIGIN      
        1 gctcaggtcg ccccggcgcg cggccagcag gtgggcggcg ccggcgagca tcgcgacgtg
       61 ggcgtcgtgc ccgcaggcgt gcatggcgcc gtcgaccgtg ctggcgtagt cgagcccggt
      121 gtcctccgga accgggagcg cgtccatggc gccgcgcagc agcaccgtcg gcccgggttc
      181 gtcgccgtcg agcacggcga cgaccgacgt ggtggtggtg cccgtggtca ccgacagcgg
      241 gaggccgtcg agcgcctcga gcaccgtcgc ctgcgtggcc ggtagctcca agccgagctc
      301 gggacgccgg tgaagccgac ggcgcaggtc gatcgcgtcc tcgagtccgg accgtgcgag
      361 gtcgagcacg ccgtcgagca cctgggtcat ccctgccgac ggtagcgatc ccgggggcta
      421 tttgatgatt ttggtgacgc ggccggcgcc gacggtgcgg ccgccttcgc ggatggcgaa
      481 gcggaggcct tcgtccatgg cgatgggatg gatgagggtg accttcatga gggtgttgtc
      541 tccgggcatg cacatctcgg tcccctcggg cagttcgacg gtgccggtca cgtcggtggt
      601 ccggaagtag aactggggcc ggtagttggc gaagaacggc ttgtgacggc cgccctcttc
      661 cttggtcaac acgtacacct gaccctcgaa ctcggtgtgg ggggtgatcg aaccgggctt
      721 gcacaacacc tggccccgct ccacctcctc tttcttcgtg ccccgcagca acgccccgat
      781 gttgtcgccc gcctggccct catcgagaag cttgcggaac atctccaccc cggtacaggt
      841 cgtggactgg gtcgggcgga tccccacgat ctccaccgtg tcaccggtgt ggatcacccc
      901 ctgttcgacc ttgccggtca ccaccgtgcc ccgcccggtg atggagaaca cgtcctcgat
      961 cggcatcagg aacggcttgt ccagctcccg gaccggctcg gggatgaaac tgtcacaggc
     1021 cgccatcaac tccaacacct gggcggccgc ctcgggctca ccatcgaggg ccttgagccc
     1081 cgacacccgc accaccggcg tgtcatcacc gggaaactcg tactcggaga gcaactcgcg
     1141 cacctcgagc tcgaccaact ccaacaactc ttcgtcatcg accatgtcga ccttgttgag
     1201 cgccaccacg atcgaaggca cccccacctg gcgggccagc aacacatgct cacgggtctg
     1261 gggcatgggc ccatcagccg ccgagacgac caggatggcc ccatcgacct gggccgcccc
     1321 cgtgatcatg ttcttgatgt aatcggcgtg gcccggcata tccacatgcg cgtaatgccg
     1381 tttatcggtc tcatactcga catgggcgat gttgatcgtg atcccccgct gcttctcctc
     1441 cggcgccttg tcaatctgat caaacgccgt gaactgcacc ttcgggttcg cctcggccaa
     1501 caccttcgtg atcgccgccg tcaacgtcgt cttcccatga tcaatatgac ccatcgtccc
     1561 cacattcaca tgcggcttcg tccgcgcaaa cttcgcctta cccatggctc acatcctctt
     1621 ccggtttccg tattcatgca gcccccctac ccccggcct
//
LOCUS       DATITW010000764         1615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_277398, whole genome shotgun sequence.
ACCESSION   DATITW010000764 DATITW010000000
VERSION     DATITW010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277398"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1615
                     /locus_tag="VLS86_10685"
     CDS             <1..>1615
                     /locus_tag="VLS86_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSO51003.1"
                     /translation="LHRLGIQAFEPVLVEGKAIQIHPMVCEAFNADFDGDQMAVHLPL
                     SAEAQAEARILMLSTNNVLRPSNGRPIITPSQDMVIGVYYLSEMVETANGAERRFGDV
                     SEAIMAYDLGQLSLHAPIRLRVGNLAGTPEKHAQFKTSLGHLITEKPVDGSVLFVTTL
                     GRALINQAFPEDFPFIDHMVAKADIGRLIEVLIEHYPRTDVATTLDAIKDLGFRFATR
                     AGLTIGLEDVKTPENKAEILADYEARADKVQSLYQKGVVTEEERRQELIEIWTEATDK
                     VKDAMEANLKMEKFNPLDMMVGSGARGNMLQVRQIAGMRGLVANPKGDIIPRPIVSTF
                     REGLSVLEYFISTHGVRKGLADTALRTADSGYLTRRLVDVSQEVIIKQVDCQTDRGIK
                     VLTVDEKGRRIRNLRSRVFGRVLADDIKVDKKLVENDDVKLKAGTLIGTKEFQLVEAT
                     ESIPELRVRSPLTCESALGVCAICYGRSLATGKLVDVGEAVGIIAAQSIGEPGTQLTM
                     RTFHTGGVAGDDITHGLPRVVELFEARVPK"
BASE COUNT          312 a          515 c          499 g          289 t
ORIGIN      
        1 gcttcaccga ctcggcatcc aggctttcga gccggtactg gtcgaaggca aggccattca
       61 gatccatccg atggtgtgtg aggcgttcaa cgccgacttc gacggcgacc agatggcggt
      121 tcacctgcca ctgtcggccg aagcgcaggc agaagccaga atcctgatgt tgtccaccaa
      181 caacgtcctg cgtccctcca acgggcggcc gatcatcact ccttcccagg acatggtcat
      241 cggcgtgtac tacctgtcgg agatggtgga gacagccaac ggagccgaac gccggttcgg
      301 cgacgtgtca gaggccatca tggcctacga cctcggccag ttgtcgctcc acgctcccat
      361 caggttgcgg gtcggcaatc tggccggtac gcctgagaag catgcgcagt tcaagacctc
      421 gctcggccat ctcatcaccg agaagccggt cgacggttcg gtgctgttcg tcaccactct
      481 gggtcgtgcc ttgatcaatc aggcgtttcc cgaggacttc ccgttcatcg accacatggt
      541 ggccaaggcc gacatcggcc ggttgatcga ggtgctcatc gagcactacc cccgcaccga
      601 cgtcgccacg accctcgacg cgatcaagga tctcggattc cgattcgcca cgagggccgg
      661 tctcaccatc ggcctggagg atgtcaagac gcccgagaac aaggcggaga tcctcgccga
      721 ctacgaagcc cgggccgaca aggtgcagtc tctgtatcag aagggtgttg tcaccgaaga
      781 ggagcggcgc caggagctga tcgagatctg gaccgaggcg actgacaagg tcaaggacgc
      841 catggaagcc aacctcaaga tggagaagtt caatcctctc gacatgatgg tcggctccgg
      901 tgcccgcggg aacatgctgc aggttcggca gatcgccggt atgcgaggcc tggtggccaa
      961 ccccaagggc gacatcatcc cgcggccgat cgtgtccacc ttccgggaag gactgtctgt
     1021 gctggagtat ttcatctcca cccacggcgt tcgcaagggt ctggccgata cggccctgcg
     1081 caccgccgac tccgggtatc tcacccggcg tctggtcgac gtttcccagg aagtgatcat
     1141 caagcaggtc gattgccaga ccgatcgcgg catcaaggtt ctaacggtgg acgaaaaggg
     1201 tcggaggatt cgcaacctgc gctcacgggt gttcggtcgg gtcctggccg atgacatcaa
     1261 ggtggacaag aagctggtcg agaacgacga cgtcaagctc aaggccggca ccctcatcgg
     1321 gaccaaggag ttccaactgg tcgaggccac cgagagcatc cccgagcttc gggtccggtc
     1381 gccactcacc tgcgagtcgg cgctcggtgt ctgcgcgatc tgctacggca ggtcgctggc
     1441 caccggcaag ctcgtcgacg tcggcgaggc ggtcggcatc atcgccgccc agtcgatcgg
     1501 tgagcccggt acccagctca cgatgcgcac cttccacacc ggtggtgtgg ctggtgacga
     1561 catcacccac ggtctgcccc gtgttgtcga gctcttcgag gcccgggtcc cgaag
//
LOCUS       DATITW010000765         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_277452, whole genome shotgun sequence.
ACCESSION   DATITW010000765 DATITW010000000
VERSION     DATITW010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277452"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VLS86_10690"
     CDS             complement(<1..1070)
                     /locus_tag="VLS86_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSO51004.1"
                     /translation="MTGLARSTVAQRVDALMNAGLVVEVGGAPSTGGRPPSLLGFNGD
                     AGLILAADLGATHSRVSVCNLLAETITEITAEVDINSGPEAVLGWLEGTFDELLATIH
                     RSADEVRAVGIGVPGPVDFSRGMAVNPPIMAGWHMYPIKERLAERHRAPVLVDNDVNT
                     MALGEYWVMEPKVDDFIFVKVGTGIGSGLILGGVLHRGANGAAGDIGHMRATSEDVVC
                     RCGNNGCLEASAGGAALAAHLRALGHSASGSRDVVKLLERGNKDATRAVREAGRLIGQ
                     VLASTVNLLNPSLIMIGGDMARADQQLLAGIREVVYQRSTTLSTTDLQITTTTLGDRA
                     GVTGAAALAIDHVMTPRAIDEAL"
     gene            complement(1273..2079)
                     /locus_tag="VLS86_10695"
     CDS             complement(1273..2079)
                     /locus_tag="VLS86_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HSO51005.1"
                     /translation="MTETDGNETGYEPKTWIDAARGLLDHIESTQLEAIDRVAGWCAE
                     AIAADGLVHLFGSGHSRIPLEEMFPRYGSFSGFHPMAELSMTFHTEVVGSNGQRQAMS
                     IERVEGLAEVILSNFEFGPNDVMIVFSAGGRSAVPIEIAMGARDRGLRVVAVTSLAES
                     MAAESGHSSRTRLLDNADIVIDIGTPAGDAMISVPGLGNPVGPGSTLANTTLVNEIKV
                     RVARILVSRGLPPVVLTSATLVGPEQSSLLFDEAYADYARRLARVLRATN"
     gene            2159..2878
                     /locus_tag="VLS86_10700"
     CDS             2159..2878
                     /locus_tag="VLS86_10700"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSO51006.1"
                     /translation="MTTRQSARYEEIARYLRGLIARASPGDRLPSDADLCQRFEVSRM
                     TARQAVQLVANDGLIERRRGAGTFVRSQPVLRDLGSPLSFSESMRMRGMIATSRILRW
                     SEIRPSEEERAALRLAPGESAHVLERLRLADGTPMAIERAVMPLDLARAIDGDLEKGS
                     LHAAFERVGRHPAQALAEVSARHPTKRQRDLLRLPASGILLCERRIISDQAGEPLERT
                     ETFYVSSRYSFRAVLVGEPRS"
     gene            2875..>3060
                     /locus_tag="VLS86_10705"
     CDS             2875..>3060
                     /locus_tag="VLS86_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51007.1"
                     /translation="MNDRHLGLDLGGTSIKVAILEQTATGFETALTDQRGTQAWLGPE
                     AVTGNLIAAAREAIASSG"
BASE COUNT          538 a         1014 c          991 g          517 t
ORIGIN      
        1 agcgcctcgt cgatggccct cggggtcatg acgtgatcga tggcgagcgc cgccgctccg
       61 gtcaccccgg cgcgatcacc caatgtggtg gtggtgatct ggaggtcggt cgtgctcagg
      121 gtcgtggatc gctggtacac cacctcgcga atacccgcca ggagctgctg gtcggctcgg
      181 gccatgtcgc ccccaatcat gatcagtgac gggttgagaa gattgaccgt cgacgccagg
      241 acctgaccaa tgagtcggcc cgcctcccgc acggctctgg tcgcgtcctt gttccccctc
      301 tccaggagct tcaccacgtc gcggcttccc gacgcggagt gacccagagc ccggagatgg
      361 gcggccagcg ccgctccacc cgctgacgcc tcgagacagc cattgttgcc acaacggcac
      421 acgacgtcct cggatgtggc gcgcatgtgg ccgatgtccc cggccgcccc gttggcgccg
      481 cggtggagca caccgccgag gatcagcccg gatccgatgc cggtccccac tttgacgaag
      541 atgaagtcgt cgaccttggg ctccatcacc cagtactcgc cgagggccat ggtgttcacg
      601 tcgttgtcga ccagcaccgg cgcccggtgt cgttcggcga gtcgctcttt gatcgggtac
      661 atgtgccagc cggccatgat cggaggattg accgccatgc cgcgtgagaa atcgaccggg
      721 ccggggaccc cgatgccgac agcgcgcacc tcatccgccg accggtggat cgtggccagc
      781 agctcgtcga aagtgccctc cagccaaccc agcaccgcct cgggcccgct gttgatgtcg
      841 acctcggctg tgatctcggt gatcgtctcg gccagcaggt tgcacaccga gacccgggaa
      901 tgggtggcac cgaggtcggc ggccaggatc agtccggcat ccccgttgaa gccgagaagc
      961 gaggggggtc gaccaccggt tgacggtgcc cctccaacct cgaccacgag accggcattc
     1021 atcagggcgt ctacgcgctg ggccacggtg gagcgagcga gcccggtcat ctcccccaac
     1081 tctccccgag tccgggcccg accggtgcgg atgagatgga gcagatctcc cggtgagtcg
     1141 gaatcgggcg ggaccgctct ctcgacactg ctcaaagttt tgccacaatg ccagagactt
     1201 ttgcgcgtgt caagtgcata agccgcgttc ttccgctctc gcgacgcatg taactggggg
     1261 cctcgccgac gatcagttgg tggctctgag cacccgagcc agacgtcggg cgtagtcggc
     1321 gtatgcctcg tcgaagagca ggctcgactg ctcgggaccg accagggtgg cgctggtcag
     1381 caccaccgga ggcaatcccc tggagaccag gatccgagcc actctcacct tgatctcgtt
     1441 cacgagcgtg gtgttggcga gggtcgaacc gggaccgacc ggattgccca agccagggac
     1501 agagatcatg gcatcgccgg ccggggtgcc gatgtcgatg acgatgtcgg cgttgtcgag
     1561 caggcgcgtc cgcgacgagt gcccggattc ggccgccatc gactcagcca gtgatgtgac
     1621 cgccaccacc cgcagacccc gatcgcgggc acccatcgcg atctcgatgg gaaccgcgga
     1681 ccgcccgccg gcgctgaaca cgatcatcac gtcgttcggc ccgaactcga agttggagag
     1741 gatcacctcg gcgagaccct cgactctctc gatagacatg gcctggcgct ggccgttgct
     1801 gccgacgact tcggtgtgaa aggtcatgga gagctcggcc atcgggtgga atcccgagaa
     1861 cgacccatat cgggggaaca tctcctcgag cgggattcgg gagtgccccg agccgaacag
     1921 gtggaccagc ccgtcggcag caatggcctc cgcgcaccag ccggccactc gatcgatcgc
     1981 ctccaattgt gtgctttcga tgtgatcgag aagaccccgg gccgcgtcga tccaagtctt
     2041 cggctcgtag ccggtctcgt tcccgtcggt ctctgtcata gctctctttc caaatgtata
     2101 tacatattat ggggacggac cgtggcgcgt tgcgaaaggc cccgaggtga gttagccggt
     2161 gacgacgaga cagtcggctc gatacgagga gatcgcccga tacctgaggg ggctcatagc
     2221 ccgtgcctcc cccggcgacc ggctgccatc ggacgccgac ctgtgccaga ggttcgaggt
     2281 gagccggatg actgctcgcc aggcagtgca actcgtggcc aacgacggtc tcatcgagag
     2341 acgacgcggg gcaggcacct ttgtccgatc ccagcccgtc ctccgggacc tgggctcgcc
     2401 cctctctttc tcggagtcga tgcggatgcg agggatgatc gcaacgtcac gaatcctgag
     2461 atggtccgag atcagaccca gcgaagagga acgcgccgcg ctgcgtctgg cacccggtga
     2521 gagcgcgcat gtcctggaac gactgagact ggcagatggg actccgatgg cgatcgagcg
     2581 ggcagtgatg cctctcgacc ttgcccgggc aatcgacggc gatctcgaga agggctcgct
     2641 gcacgctgcc ttcgagcgcg tcggtcgcca cccggcccag gcgctggccg aagtatcggc
     2701 caggcacccc accaaacgcc agcgcgacct gcttcgcctc cctgcttcgg ggattctcct
     2761 ctgtgagcgg aggatcatca gcgatcaggc cggagagcct ctggaaagga ccgagacgtt
     2821 ctacgtgtcg agtcgctaca gcttccgggc cgtcctggtc ggagagccgc gatcgtgaac
     2881 gatcgacatc tgggactcga tctgggcggc acgagcatca aggtcgcgat cctcgagcag
     2941 accgccaccg gtttcgagac agcgctcacc gatcaacgag gcacccaagc ctggctgggt
     3001 ccggaggcag tgacggggaa cctgatagct gccgcccgcg aggcaatcgc ctcctccggg
//
LOCUS       DATITW010000766         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_117834, whole genome shotgun sequence.
ACCESSION   DATITW010000766 DATITW010000000
VERSION     DATITW010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117834"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VLS86_10710"
     CDS             complement(<1..538)
                     /locus_tag="VLS86_10710"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSO51008.1"
                     /translation="MDLGWIEILILIVVVLLGFFAKGVTGIGAPLLIVPIMAGFTGLE
                     FAVAVIAIPTVVANTWLLWRTRGSVRDVAWFLWPLLVAGALGTVIGSWILVNLDDRIM
                     TVTLAVIVLAYIAWSMANRDFELSDVWARRLSAPAGLFAGVLQGGTGASGPIVATYVH
                     ALNLDRAAFVLAVTIPFQV"
     gene            complement(609..1166)
                     /locus_tag="VLS86_10715"
     CDS             complement(609..1166)
                     /locus_tag="VLS86_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase"
                     /protein_id="HSO51009.1"
                     /translation="MARIPYPDLDSVTDPEILAILERARQHGTPRPESQAIRAHVPAV
                     LKAFSTAWQDTFVDGVVDHALKELCRVYVTKTVDCGYCAAQRSVDSPTPEEHYSELLE
                     YRTSDRYTDREKAALAYTDAIVWNPDLADDEVWDALHANFTDPELVELGFFIALTSGQ
                     QRWIKTLGLGHREVLADTEAGLAVR"
     gene            complement(1519..1710)
                     /locus_tag="VLS86_10720"
     CDS             complement(1519..1710)
                     /locus_tag="VLS86_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51010.1"
                     /translation="MRLHKGTLFAGVIYLGIGIAFALEAMEVWTLRIGDLRFVAPLAL
                     VVVGLAVVIGSLGRAERQT"
     gene            complement(1707..>2217)
                     /locus_tag="VLS86_10725"
     CDS             complement(1707..>2217)
                     /locus_tag="VLS86_10725"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HSO51011.1"
                     /translation="ELRAAMVLPVVLAVIGLALIIGAFDGPHTGLVVAGVFVSIAVLA
                     VAAAPPDAFHGGIGERDIRVTDQANLAPRYDVGVGEMRLDLGDLEMTYSDAVDVTVGA
                     GDLTILLPPDVPVDIDASVGAGQVNLLGETSDGLSVNRTYTSDGFESAAVRLTLDLDV
                     AAGNIRVDR"
BASE COUNT          425 a          718 c          727 g          347 t
ORIGIN      
        1 acacctggaa ggggatggtc accgccagca cgaatgccgc ccgatccagg ttgagggcgt
       61 ggacataggt ggcgacgatc gggcccgatg cgccggttcc tccctgaaga acgcctgcga
      121 acagcccggc cggcgccgag agacgacggg cccagacgtc gctcagctcg aaatcacggt
      181 tggccattga ccaggcgatg taggcgagga cgatgacggc gagggtgacg gtcatgatcc
      241 ggtcgtcgag gttcaccagg atccaggatc caatcactgt gcccagggcg ccggcgacga
      301 gaaggggcca gagaaaccaa gcaacgtcgc ggaccgagcc ccgggtgcgc cacaacaacc
      361 aggtgttggc cacaacggtc gggatcgcga tcactgccac cgcgaactcg agaccggtga
      421 agccggccat tatcggaacg atgagcaacg gggcaccgat ccccgtcacg cccttggcaa
      481 agaacccgag gagcacgacc acgatcaaga tgaggatctc gatccatccc agatccatga
      541 ggtgccctga ctacgccctg gtcagaccac ttcgtcgcgt cgagcaagga cgacgagcgg
      601 gactgctctc aacgaacggc cagaccggcc tcggtgtccg ccaacacctc gcggtggccc
      661 aacccgaggg tcttgatcca tctctgctga ccggaggtga gggcgatgaa gaagccgagc
      721 tcgaccagct ctgggtcggt gaagttggca tggagcgcat cccagacctc gtcgtcggcg
      781 agatccgggt tccagacgat ggcgtcggtg taggccaggg ccgccttctc ccggtcggtg
      841 tagcggtcgg aagtccggta ctcgagaagc tcggagtagt gctcctccgg agtcggcgag
      901 tcgaccgaac gctgggcggc gcaatacccg caatccaccg tcttggtcac gtagacccgg
      961 cacagctcct tgagggcgtg atcgacgacc ccgtcgacga aggtgtcctg ccaggcggtg
     1021 gagaaggcct tgagcacagc agggacatga gctcggattg cctgagattc ggggcgcggc
     1081 gtcccgtgct gtctcgcccg ttccaggatg gccaggatct cgggatcggt cacgctatcg
     1141 aggtcggggt acgggattcg ggccacggct gttctccgat cgaagacttg gagctgccca
     1201 gcctaggcgg gccacgagct cagccctcga tgacccggtc ccaccagtag cgaatcatca
     1261 gcgccgcctc cggcccggcg tcgtcctgcc agtggtgacc ggcgtgacgc aggttgtagg
     1321 tccagcggtt ggagaggatc gaatcccatc gtctcttctc ggtgccccgg aacgcggggt
     1381 cctggtcggc ccagaccagg agggtcggcc acagtgacca gcctccgatc ggttggcccg
     1441 ccggttagag cgctctcagg ggacggcaac gcagaaggcc ccgcccgtag gagcgcgggg
     1501 cctcctgttt ggatgggctc aggtctgtcg ctctgcccgc cccagcgagc cgatgacgac
     1561 cgcgagcccg acaaccacga gggcgagagg ggccacgaac cggaggtctc cgatccgaag
     1621 cgtccacact tccatggctt ccaaggcgaa tgcgatcccg atccccaggt agatcactcc
     1681 tgcgaacaag gtccctttgt gaagtctcat ctgtccaccc tgatgtttcc ggctgccaca
     1741 tcgaggtcga gtgtcagcct caccgcggcg gattcgaatc cgtcactggt gtaggtgcgg
     1801 tttaccgaaa ggccatcact ggtctctccg agcaggttca cctgacctgc gccgaccgag
     1861 gcgtcgatgt cgaccgggac gtccggaggc aggaggatcg tgagatctcc ggcaccgacc
     1921 gtcacatcga cagcgtccga gtaggtcatc tccagatcac cgagatcgag tctcatctcg
     1981 ccgaccccga cgtcatatcg gggagcgagg ttggcctggt cggtgacccg aatgtcgcgt
     2041 tcaccgatcc ccccatggaa ggcgtccggc ggcgccgcgg ccaccgccaa cacggcgatg
     2101 ctcacgaata ccccggcgac gacgagcccg gtatggggcc cgtcgaaggc accgatgatc
     2161 aatgccaggc caatgacggc gagcaccacg ggcaggacca tcgccgcccg cagttcg
//
LOCUS       DATITW010000767         1943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_77732, whole genome shotgun sequence.
ACCESSION   DATITW010000767 DATITW010000000
VERSION     DATITW010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77732"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..792
                     /locus_tag="VLS86_10730"
     CDS             208..792
                     /locus_tag="VLS86_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011790823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51012.1"
                     /translation="MTELLYQALETELGGVQVYETALRCTVNEDLREEWQGYLEETEN
                     HVRIVSDVFAELDLDPEVETPGRAIVRFKGEALITAMQMALDAGDMGAAQLVAAECVV
                     DAEIKDHANWELIGEVASELKGDMAKVLQDAYEQVEEEEDEHLYHTQGWARELWFESL
                     GMTAVLPPPEEIKKVKTAIGAARAEQSREDYIDS"
     gene            968..1897
                     /locus_tag="VLS86_10735"
     CDS             968..1897
                     /locus_tag="VLS86_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HSO51013.1"
                     /translation="MVRRRLYIVLALSLVLAACGDGASDDTSTTAGGGETTTAPPAET
                     TTTAAETTTTAADVCSTTSEALFEPGSLTIATGEPVFPPWMIDDDPTNGQGFESAVAY
                     ALAEELGFAAEDVNWVRTTFEEAIAPGERPYDFNMQQYGITEERDEIVDFSIPYYVEQ
                     KTVVALEGTAAASATTFAELAESNLGATIGTTDLDYIVEVIGVPDAEVAVYNDQAATF
                     AALAAGQIDATVIGLSTAYFITAVEIDNAVISGVLPDVGGTEGGMGLLFTDGNPYVEC
                     VNAALQTLTDDGTLTALGDEWLEAGGDIPEITE"
BASE COUNT          383 a          636 c          648 g          276 t
ORIGIN      
        1 ctcgtcgtag tcgtggtggc gtcggcgccg gacgaggagc acgccacgat cagcagcagg
       61 agtaagggga gagacctggt catcggatcg cgatcttatg gccggccccc gcattggttt
      121 ggacttcacc ggcctcgggt aagcgccgct accgccacca gacgtggcgc aaactcgcct
      181 aggaggaaaa acgtgaatga tgcacaagtg accgagctcc tgtaccaggc cctggagacg
      241 gagctgggtg gggttcaggt ctacgagacc gcccttcgct gcacggtcaa cgaggacctg
      301 cgtgaagagt ggcagggtta cctggaggag accgagaacc acgtacgcat cgtcagcgat
      361 gtcttcgccg agctcgacct cgatcccgag gtggagaccc cggggcgggc aatcgtccgt
      421 ttcaagggcg aggcgctgat caccgcgatg cagatggcgc tcgacgccgg tgacatgggc
      481 gcggcccagc tggtcgccgc cgagtgtgtg gtcgacgccg agatcaagga ccatgccaac
      541 tgggagctga tcggcgaagt cgcctcagag ctcaagggag acatggcaaa ggtgctccag
      601 gacgcctacg agcaggtgga ggaagaagag gacgagcatc tctatcacac ccagggttgg
      661 gcgcgagagc tctggttcga gtccctgggc atgaccgccg tcctgccgcc acccgaagag
      721 atcaagaagg tcaagaccgc gatcggggcc gccagggccg agcagtcgcg cgaggactac
      781 atcgacagct aaccgccggg tccacgtgag ccctgcgctc cgccccccgg gcaggagcgc
      841 agggccgtcg gcggagaggc gacgacgcag gcttagaatc cacgccgctg ctagcgcagt
      901 cggtcccgac ccacgcacag tgggatgggc tcgtacctga gacaccctga acgaggaggc
      961 acctgcgatg gttcgcagac gcttgtacat agtcctggca ctctccctgg ttctggctgc
     1021 gtgtggcgac ggagcatccg acgacaccag cacgacggcc ggaggcggcg agaccaccac
     1081 cgcgccaccg gcggagacca cgaccaccgc agccgagacg accaccacgg cagcagacgt
     1141 ctgctcgacg accagcgagg cgctgttcga gccggggagc ctgaccatcg ccaccggtga
     1201 gccggtgttc ccgccatgga tgatcgacga cgacccgacc aacggccagg gattcgaaag
     1261 cgccgtcgcc tatgcgctgg ccgaggagct cgggttcgcc gccgaggacg tgaattgggt
     1321 ccgcaccacc tttgaggagg ccatcgcccc cggtgagcgg ccctacgact tcaacatgca
     1381 gcagtacggg atcaccgagg agcgcgacga gatcgtcgac ttctcgatcc cctactacgt
     1441 cgagcagaag accgtcgtgg ccctcgaagg caccgcggcc gccagcgcga ccaccttcgc
     1501 cgagctcgca gagtcgaacc tcggcgcaac catcggcacc accgacctcg actacatcgt
     1561 ggaggtgatc ggtgtccccg atgccgaggt agcggtctac aacgaccagg ccgccacctt
     1621 cgccgcactc gccgccggtc agatcgacgc cacggtgatc ggtctgtcca cggcctactt
     1681 catcaccgcc gtcgagatcg acaacgcggt gatctcgggt gtcctccccg acgtgggagg
     1741 aaccgagggt gggatgggtc tcctgttcac cgacgggaac ccgtacgtcg agtgcgtcaa
     1801 cgccgcgctc cagaccctga ccgacgacgg gactctcacg gcactcggtg acgagtggct
     1861 ggaggccggc ggggacatcc cggagataac tgagtgacag aggagcgttc cgcgccctcc
     1921 cggcgcacac ggaacgctcg tga
//
LOCUS       DATITW010000768         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_598347, whole genome shotgun sequence.
ACCESSION   DATITW010000768 DATITW010000000
VERSION     DATITW010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598347"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VLS86_10740"
     CDS             complement(<1..195)
                     /locus_tag="VLS86_10740"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HSO51014.1"
                     /translation="MPRVAILGTGLIGASVGLALKEQGCDTVGWDPSSDALERAAERH
                     ATDSTALSVEEAVEDADLIVL"
     gene            complement(199..1113)
                     /locus_tag="VLS86_10745"
     CDS             complement(199..1113)
                     /locus_tag="VLS86_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSO51015.1"
                     /translation="MEYAVQTRGHWGLILTTARWAEDRGLVALALPDHYLERGDAAER
                     PAFDHLIHLAALARETTDLELVSLLSPVTFRHPAVFYKMAVTLDEISGGRFTLGVGTG
                     GLDDEFELFGIPYPDRRTRFELLDECMAYLSAALAPEPRGFAGTHYRLAEFDPHPHPL
                     NLRLLVGGGGEVKTPQLAGRYADEFNIYACPPDEYVSKLELARATASESGRDPDAILF
                     STACPAIAASAEADYRRLLQLLAEQTKSTPERIEHVYEQRGYPHGSGSKPSEMLHALE
                     EAGCQRFYPQMFLGNPADYDTILDAYLG"
     gene            complement(1144..1704)
                     /locus_tag="VLS86_10750"
     CDS             complement(1144..1704)
                     /locus_tag="VLS86_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSO51016.1"
                     /translation="MRYKAGLETRDRILEAARALIAANGLEGTTIKAICQEAGVLPGS
                     FYNLFPSKEQAILTVVREAIDAVDPDPAHQGIDTLDELVSAYVRFLVEQPDLSRVYIR
                     IGVSGAGSSPELTGRMVRHHQGRVARFAAAMKRESPDLAETLAERRAETLVLALNGIA
                     LHRVIDPEFDVAWHARALLEDAAAVS"
     gene            1825..>2694
                     /locus_tag="VLS86_10755"
     CDS             1825..>2694
                     /locus_tag="VLS86_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSO51017.1"
                     /translation="MSHYKSNLRDLEFNLFEVNRIQDYLGTGEWPDCDEATARDILVE
                     LERLSREDFAASYVDQDRIPLVLDNGEVRLPESVLAGVRAFKEGGWDRLGVPAEMDGM
                     PVPPSLGWATQEMQLAANTTVSWYSGGALFARVLFEEGTEAQKELARLWLDKQWSGSM
                     ILTEPDAGSDVGAAVTKAIDMGDGTWHIEGVKRFITGGEHDAAENILHLVLARPEGAI
                     AGTKGLSLFVVPKYLINDDGSLGERNGVYATNLEKKMGLKGSTTCEMTFGLDHPAIGY
                     LVGDRHDGIRQMFR"
BASE COUNT          481 a          825 c          908 g          480 t
ORIGIN      
        1 caacacgatg agatcggcat cctcgacagc ttcttccacc gatagggcgg tgctgtcggt
       61 ggcgtgccgc tcggctgccc tttccagcgc gtcagacgac gggtcccacc caacggtgtc
      121 acacccctgc tctttcaatg cgagcccgac ggaggcgccg atcagcccgg ttccgaggat
      181 tgctacgcgt ggcatggatc agccgaggta ggcgtcgagg atcgtgtcgt agtcggctgg
      241 gttgcccaag aacatctgag gatagaaccg ttgacagccg gcctcttcca gggcgtgaag
      301 catctcggag ggcttggacc cggagccatg tgggtacccc cgctgctcgt agacatgctc
      361 gattcgctcc ggcgtggatt tggtctgctc ggcgagcagc tggagcagac gccgatagtc
      421 ggcttccgcg ctggccgcga tcgcaggaca ggcggtggag aagaggatgg cgtcgggatc
      481 tcgccccgac tccgatgcgg tggccctcgc cagctccagc ttcgagacgt actcgtcggg
      541 agggcacgcg tagatgttga actcgtcggc gtagcgaccg gccagctgcg gggtcttgac
      601 ctcgccacct cctccgacga gcagtctcag gttcagaggg tggggatgtg ggtcgaactc
      661 ggcgagtcga tagtgggtcc cggcgaagcc gcgcggctcc ggagccagcg ctgcggacag
      721 ataggccatg cactcgtcga gcaactcgaa tcgggtccga cgatcagggt acgggatgcc
      781 aaacagctcg aactcatcgt cgagcccacc ggtcccaacg cccagggtga accgcccgcc
      841 tgagatctca tcgagggtca ccgccatctt gtagaagacc gcagggtggc gaaatgtgac
      901 cggcgacaac agggaaacca gctcgaggtc tgtggtttcc cgggccaggg ctgcgaggtg
      961 gatcagatgg tcgaacgcgg ggcgctccgc ggcatcgcct cgttccagat agtggtcggg
     1021 gagtgccagt gcgaccagac ctcggtcctc ggcccatctc gcagtggtga ggatcagccc
     1081 ccagtggccg cgggtctgca ctgcgtattc catcgataga gcctattcgg gggtcggatc
     1141 gagtcaggaa acggcggccg cgtcctcgag caaggcgcgt gcgtgccagg cgacgtcgaa
     1201 ctcggggtcg atgacccgat gcagggcgat gccattgagc gccagcacca gggtctccgc
     1261 ccgtctttcg gccagggtct cggcgagatc ggggctctcg cgtttcatcg ccgctgcgaa
     1321 acgggcgacc cttccctggt gatggcggac catccgacct gtcagctcgg ggctggaacc
     1381 ggcgccggag acaccgatgc ggatgtagac ccgcgacaga tcgggctgtt cgaccaagaa
     1441 gcggacgtag gcgctcacca gctcgtcgag ggtgtcgatg ccctggtgcg caggatcggg
     1501 gtcgacggca tcgatcgcct cgcggaccac ggtgaggatg gcctgctcct tggacgggaa
     1561 caggttgtag aaggatccgg gcaggacccc cgcctcctgg catatcgcct tgatcgtcgt
     1621 cccctcgagc ccgttggcgg caatgagagc ccgggcggcc tccagaattc ggtcacgggt
     1681 ctcgagaccg gccttgtaac gcatgtcgga acggtaatgc ctcgtgggca cgacaaccgt
     1741 cgcgatcggc tcgtggaacc gactactttc tttgagcgtt gattcaaatt agagtattca
     1801 ctcaaagaac gggagacggt cggcatgagc cattacaaat cgaatctccg tgatctcgaa
     1861 ttcaacctct tcgaggtcaa tcggattcag gactatctag gcaccggaga gtggcctgac
     1921 tgcgacgaag cgaccgcccg tgacatcctg gtcgagttgg aacgtctctc ccgtgaggac
     1981 ttcgctgcgt cctacgtcga ccaggatcga atcccgctcg tcctcgacaa cggcgaggtc
     2041 cgtcttcccg agtcggtgct ggccggggtc cgtgccttca aggagggtgg ctgggatcgt
     2101 ctcggggtcc ccgccgagat ggacgggatg ccggtccccc cgagtctggg ttgggcgacc
     2161 caggagatgc agctggctgc caacaccacc gtttcgtggt actccggcgg tgcgctcttc
     2221 gcccgtgtcc tgttcgagga gggaaccgag gcccaaaagg agctggcaag gctctggctc
     2281 gacaagcaat ggtccgggtc gatgatcctc accgagcccg acgccggctc ggacgtcggc
     2341 gccgccgtca ccaaggccat cgacatgggc gatgggacct ggcacatcga gggcgtcaag
     2401 cggttcatca ccggtgggga gcacgacgcc gccgagaaca tcctgcatct ggtgctggcc
     2461 cgtcccgagg gtgcgatcgc cggcaccaag gggctctcgt tgttcgtcgt ccccaagtac
     2521 ctgatcaacg acgacggcag ccttggtgag cggaacggcg tgtacgccac gaacctcgag
     2581 aagaagatgg gcttgaaggg ctccaccacg tgcgagatga cgtttgggtt ggatcacccc
     2641 gcgatcggct atctggtcgg cgaccggcac gatggcatcc ggcagatgtt cagg
//
LOCUS       DATITW010000769         1891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_438246, whole genome shotgun sequence.
ACCESSION   DATITW010000769 DATITW010000000
VERSION     DATITW010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438246"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1891
                     /locus_tag="VLS86_10760"
     CDS             <1..>1891
                     /locus_tag="VLS86_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSO51018.1"
                     /translation="VKKPETINYRTLRPEKEGLFDERIFGPQRDWECYCGKYKRIRYK
                     GIICERCGVEVTRSKVRRERMGHIELAAPVTHIWFFKGVPSRLGYLLDMSPKELEKVI
                     YFASYIITSVDDEKRQKDLPELEAAMRDEIEVVRNEFNEWHGVRLEALEDELERMEEG
                     GAKSQEINARKKEVEREIKDRQERSDREIELIEDAYDTFRKFKPKDLVESEQLWREIY
                     FRYADYFEGGMGAEAVKALLLRLDLDTEMTLLEEHLEAGSAQKRQRATQRMKVVKPFW
                     EGQNRPEDMILETVPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLG
                     APEIIVNNEKRMLQEAVDALFDNGRRGRAVTGPGNRALKSLSDMLKGKQGRFRQNLLG
                     KRVDYSGRSVIVVGPQLKLHQCGLPKIMALELFKPFVMKRLVDLDLAQNIKAAKRMVE
                     RQRPQVWDVLSDVIQEHPVLLNRAPTLHRLGIQAFEPVLVEGKAIQIHPLVCEAFNAD
                     FDGDQMAVHLPLSAEAQAEARILMLSSNNVLSPASGRPIVTPSQDMVIGIYYLSEHVE
                     DATGAGRYFVDFDEALMAHEIGELSLHAPIKIRLGELAGDPELHADLKPLLGTLLPEE
                     PMSSVG"
BASE COUNT          376 a          604 c          604 g          307 t
ORIGIN      
        1 aggtcaagaa gccggagacg atcaactacc ggaccctgcg acccgagaag gaagggctgt
       61 tcgacgagcg catcttcggt ccgcagagag actgggagtg ctactgcggc aagtacaaga
      121 ggatccgcta caagggcatc atctgtgagc gctgcggggt cgaggtcaca cggtccaagg
      181 ttcgtcggga gcgcatgggc cacatcgagt tggcggcgcc ggtcacccac atctggttct
      241 tcaagggcgt gcccagccgt ctcggctatc tgctcgacat gtcgcccaag gagctggaga
      301 aggtcatcta cttcgccagc tacatcatca cctcggtcga cgacgagaag cggcagaagg
      361 acctgcccga gctcgaagcc gcgatgcgcg acgagatcga agtggtccgc aatgagttca
      421 acgaatggca cggagtccgt ctcgaggccc tcgaggacga gctggagcgc atggaggaag
      481 gcggggccaa gagccaggag atcaacgctc gaaagaagga agtcgagcgg gagatcaagg
      541 accgccagga gcgatccgat cgcgagatcg agctgatcga ggacgcttac gacacgttcc
      601 gcaagttcaa gcccaaggat ctcgtcgagt ccgagcagct ttggcgcgag atctacttcc
      661 gctacgccga ctacttcgag ggcgggatgg gcgccgaggc ggtcaaggcc ctgttgctcc
      721 gtctcgacct cgacaccgag atgaccctgc tcgaggagca tctggaggca ggttcggccc
      781 agaagcggca gcgggcaacg cagcggatga aggtggtcaa gccgttctgg gagggtcaga
      841 accgtcccga ggacatgatc ctggagaccg tcccggtcat cccgcccgac ctgcggccga
      901 tggtccagct cgacggcggc cgcttcgcca cctccgacct gaacgacctc tatcgccggg
      961 tgatcaaccg gaacaaccgg ctcaagcggc tgctcgacct cggcgccccc gagatcatcg
     1021 tcaacaacga gaagcgcatg ctccaggagg ctgtcgacgc cctgttcgac aacggccgcc
     1081 gcgggcgagc ggtgaccggc ccgggcaacc gcgccctcaa gtccctctcc gacatgctca
     1141 agggaaagca ggggcggttc cgtcagaacc tcctcggcaa gcgcgtcgac tattccggtc
     1201 gctcggtcat cgtcgtcggc cctcagctca agctgcatca gtgcggtctg cccaagatca
     1261 tggccctgga gctgttcaag ccattcgtga tgaagcgtct ggtcgatctc gacctggccc
     1321 agaacatcaa ggccgcaaag cggatggtgg agagacagcg accccaggtc tgggacgtcc
     1381 tctccgacgt catccaggag cacccggtgc ttctcaaccg ggcgccgacc ctgcaccgtc
     1441 tgggcatcca ggcgttcgag ccggtcctgg tcgagggcaa ggcgattcag atccacccct
     1501 tggtgtgtga ggcgttcaac gccgacttcg acggcgacca gatggcagtc catctcccgc
     1561 tgtcggcaga ggcccaggct gaggccagga tcctcatgct gtcctcgaac aacgtcctct
     1621 cgcctgcttc gggacggccg atcgtcaccc cttctcagga catggtgatc gggatctact
     1681 acctatccga gcacgtcgag gatgccacag gggccggccg ctacttcgtc gatttcgacg
     1741 aggcgctgat ggcccacgag atcggcgagt tgtcgctgca tgccccgatc aagatcaggc
     1801 tcggcgagct ggccggcgat cccgagctgc acgccgacct caagccgctg ctgggaaccc
     1861 tccttcccga ggagccgatg agctcggtcg g
//
LOCUS       DATITW010000770         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_477986, whole genome shotgun sequence.
ACCESSION   DATITW010000770 DATITW010000000
VERSION     DATITW010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477986"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1906
                     /locus_tag="VLS86_10765"
     CDS             <1..1906
                     /locus_tag="VLS86_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006550221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HSO51019.1"
                     /translation="DFAKIVNRLQRDVHYELDERKRQVLTTEEGVARVEQVLGVENLF
                     DHAAVDFVHHLEAALKAKELFLRDVDYLIDHGEVKIVDEFTGRVLEGRRYSEGLHQAI
                     EAKEGVAIKEENQTLATITLQNYFRLYDKLAGMTGTAETEAAELAQIYSLEVIAVPTN
                     QPVARDDEGDLVYKTEDGKFGAVVADVTARTEIGQPVLLGTVSIEKSERLSKAFDKHG
                     IKHEVLNAKQHSREAEIIAQAGRPGAVTVATNMAGRGVDIMLGGNPDGMARAELKRRG
                     ITPDDPSYKALEDKLTEEFATQIASDKQKVIDAGGLYVVGTERHDSRRIDNQLRGRSG
                     RQGDPGEARFYLSLEDDLMRRFQGEWVTSIMDRLRLPEDQPIEANMVSKAIERAQRQV
                     ESQNFEIRKNVLKYDEVMNRQREVIYDWRRSILEGTAEDDLIGDWIEGVVSEVVETEL
                     SPGIPQSEWDWETVQRELTQFYPTEIESSKVAGLDAAGVVDYVVEEALDAYARREEDL
                     GSEVLRQVERSVMLSVIDNKWREHLSDMDYLRAGIGLRAMGQRDPLSEYQREAYDMFT
                     ELVETVKRDAVRYLFRVELAKPKTEPQRVAANPTSSGPKKPAVSDKIGRNDACPCGSG
                     KKYKRCHGAA"
     gene            1985..>2604
                     /locus_tag="VLS86_10770"
     CDS             1985..>2604
                     /locus_tag="VLS86_10770"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HSO51020.1"
                     /translation="MTQNQEITDPKAMLAELRQRLVEARGFLDLEAKERELAELRDKA
                     SSPDLWDHPDEARTVSQRLARYEGLFDMVTDLTSKIDDAEVLLELADEAEDAASRTEA
                     LAELSGVSGQLDALELESLYFDEYDDADAILSVHAGAGGVDAQDWAQMMARMYQRFLA
                     DAGFSITVEEVTEGEEAGIKSATFTVKGDRAYGTLESERGVHRLVR"
BASE COUNT          534 a          793 c          876 g          401 t
ORIGIN      
        1 ggacttcgcc aagatcgtca accgtctcca gcgggacgtc cactacgagt tggacgagag
       61 gaagcgtcag gtgctcacca ccgaggaagg cgttgcccga gtcgagcagg tccttggtgt
      121 ggagaacctg ttcgaccatg ccgcggtcga cttcgtccac catctggagg cggcgctcaa
      181 ggccaaggag ctgttccttc gcgatgtgga ctacctgatc gaccacggcg aggtgaagat
      241 cgtcgatgag ttcaccgggc gtgtcctcga aggccgtcgc tactcggaag gccttcatca
      301 ggccatcgag gccaaagaag gggtggcgat caaagaggag aaccagaccc tggccacgat
      361 cactctccag aactacttcc ggctctacga caagctggcg ggaatgacgg ggacggcgga
      421 gaccgaggca gccgagctcg cccagatcta ctcgctcgag gtgatcgcgg tgcccaccaa
      481 ccagccggtg gcccgtgacg acgaaggcga cctggtgtac aagacggagg acggcaagtt
      541 cggtgcagtg gtggccgacg tgaccgcccg caccgagatc ggccagccgg tcctgctcgg
      601 caccgtctcc atcgagaaat cggagaggct gtcgaaggcg ttcgacaagc acgggatcaa
      661 acacgaggtg ctcaacgcaa agcagcactc acgtgaggcc gagatcatcg cccaggccgg
      721 acgtcccggg gcggtcaccg tcgccaccaa catggccggt cgcggtgtcg acatcatgct
      781 cgggggaaac cccgacggca tggcgcgggc cgagctcaag cggaggggca ttactccgga
      841 cgacccgtcc tacaaggcac tcgaagacaa gctcaccgag gagttcgcca cccagatcgc
      901 atccgacaag cagaaggtga tcgatgccgg cggtctctat gtcgtgggca ccgagcgcca
      961 tgattcgagg cggatcgaca accagctccg tggtcgatcg gggcgccagg gcgaccctgg
     1021 ggaggcgcgt ttctatctct cgctcgaaga cgacctgatg cgtcgtttcc agggggagtg
     1081 ggtcacctcg atcatggacc ggctgcgtct tcccgaggac cagccgatcg aggccaacat
     1141 ggtgtcgaag gcgatcgaga gggcgcaacg ccaggtggag tcgcagaact tcgagatccg
     1201 caagaacgtc ttgaagtacg acgaggtgat gaaccgccag cgtgaggtga tctacgactg
     1261 gcggcgctcg atcctggaag ggaccgcaga ggacgacctg atcggcgact ggatcgaggg
     1321 cgtggtcagc gaggtggtcg agaccgagct gtcgccgggc attccccagt cggaatggga
     1381 ctgggaaacc gtgcagcggg aattgaccca gttctacccg accgagatcg agtcatcgaa
     1441 ggttgccggg ctcgatgccg cgggggtggt cgattacgtg gtcgaagagg ctctcgacgc
     1501 ctacgcccgg cgggaggagg acctcggctc cgaagtcctg cgccaggtcg agaggtcggt
     1561 gatgctctcg gtcatcgaca acaagtggcg cgagcatctc tcggacatgg actatctgcg
     1621 cgccgggatc gggcttcggg cgatgggcca gcgcgatccc ctgtcggagt atcagcgcga
     1681 ggcatacgac atgttcaccg aactggtgga gaccgtgaag cgggacgctg tgcgctatct
     1741 gttccgggtc gagctggcca aacccaagac cgagccgcaa agggtggcgg ccaaccccac
     1801 cagcagcggg ccgaagaagc ctgcagtctc ggacaagatc ggtcgcaacg acgcctgccc
     1861 ttgcggatcg gggaagaagt acaagcgctg ccacggagca gcttgagcgg tctcacccct
     1921 caccggccct gatcagcccc acggcccggc catccggcgg gccacaactc acaacccacg
     1981 acccatgacc cagaaccaag agataaccga tcccaaggcg atgctcgccg agttgcggca
     2041 gcgtctggtc gaggcgcggg gattcctcga tctcgaggcc aaggagagag agctggccga
     2101 gctcagggac aaggcatcat cccctgatct ctgggaccat cccgacgagg cgcggaccgt
     2161 cagtcagcgt ctcgcccgct atgagggcct gttcgacatg gtcaccgatc tcacctcgaa
     2221 gatcgacgac gccgaagtgc tgttggaact ggccgacgaa gcggaggatg ctgcatcacg
     2281 caccgaagcg ctggccgagc tctcgggtgt ctcaggacag ctcgacgccc tcgagctgga
     2341 gtcgctgtac ttcgacgagt acgacgacgc cgacgccatt ctcagcgttc acgccggcgc
     2401 gggcggagtc gacgctcagg actgggcgca gatgatggcc cggatgtacc agcggttcct
     2461 cgccgacgcc ggtttcagca tcaccgtcga ggaggtgacc gaaggcgagg aagccgggat
     2521 caagtcggcg actttcaccg tgaagggtga tcgcgcctat gggactctgg agtccgagcg
     2581 cggggtccat cgactggtcc ggat
//
LOCUS       DATITW010000771         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_758559, whole genome shotgun sequence.
ACCESSION   DATITW010000771 DATITW010000000
VERSION     DATITW010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758559"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1121
                     /locus_tag="VLS86_10775"
     CDS             99..1121
                     /locus_tag="VLS86_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSO51021.1"
                     /translation="MEEGAVLVTGALGCIGAWTVRELVMSDVPVVAFDRSTDPRRLRQ
                     ITTQEQFERVRLIQGDLTDLANLQETIVANEVDRVVHLGALQIPFCRADPPLGALVNV
                     VGTVNVFEAARRAGIPHIVYTSSIAVFDWGPERGRLTVDAPARPVSHYGAYKLANEGT
                     ARAYWADFGVSSIGIRPMTVYGPGRDQGLTSSPTRGILAAVLGHRYEIGFGGFTLFHY
                     AGDVGRALAVAANTPGAGAHVVNLNGVRAPISNVVASLHDLVGSSAEGITVGPNALPF
                     PDDVDTSGLDIIGPPSVTPLNEGIAATVEFFRDLQSRGALEVVEHGLVVGDGVVLDRE
                     SVPSTE"
     gene            1127..1891
                     /locus_tag="VLS86_10780"
                     /pseudo
     CDS             1127..1891
                     /locus_tag="VLS86_10780"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            complement(2033..>2350)
                     /locus_tag="VLS86_10785"
     CDS             complement(2033..>2350)
                     /locus_tag="VLS86_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSO51022.1"
                     /translation="GMIATTLRNAKTAGINDGGAILHAEGVFHESRIRSDLEIYDRVG
                     GGDGFASGMIWALLEGRSPAEAVEIGAAHGALTMTTPGDTSMVTSSEVMRAVVAHGAR
                     VVR"
BASE COUNT          405 a          777 c          743 g          425 t
ORIGIN      
        1 gccccgggga cgagcatcac catgcattct tccagcatca gcgccttttc ctacccgttc
       61 ggggctgaat ggtcgggacc cctggtcgac ggcacctgat ggaagaaggt gcagtcctgg
      121 tcacgggggc cttgggctgt atcggggcat ggactgttcg tgagctggtg atgagcgacg
      181 ttccggttgt ggccttcgat cggtccaccg acccgagacg gctgcggcag atcacgaccc
      241 aggaacagtt cgagagggtc cgtttgatcc agggagacct cacagacctc gccaacctcc
      301 aggagacaat cgtcgccaac gaggttgaca gggtggtcca tctcggggcc cttcagatcc
      361 ccttttgccg ggccgacccc ccacttggcg cactggtcaa cgtcgtggga accgtgaacg
      421 tcttcgaggc cgcccgtcgg gctggcatcc cgcacatcgt gtatacgagc tcgattgcgg
      481 tcttcgactg gggccccgag cgcggtcgtc tcacggtcga tgctccggca cgacccgtca
      541 gccactatgg cgcctacaag ctggccaacg aaggcactgc ccgcgcctac tgggctgatt
      601 tcggtgtgtc gagcatcggt atcaggccaa tgacggtgta tgggccgggg cgggaccagg
      661 ggctcaccag ctcaccaacc agaggcatct tggccgccgt gctcggacac cggtatgaga
      721 tcggtttcgg tggtttcacg ctgttccact acgccggaga cgtgggtcgt gcgctggccg
      781 tggccgcaaa cacgccgggc gccggggcac atgtggtgaa cctgaacggc gtgcgcgccc
      841 ctatctccaa cgtcgtcgcc tccttgcatg atctcgtcgg ctcatcggcg gaggggatca
      901 ccgtcggccc gaatgccctg cccttcccgg acgacgtgga cacatcgggg ctcgacatca
      961 tcgggccgcc atccgtgaca ccgctcaacg aaggcatcgc cgccaccgtg gagttctttc
     1021 gagacctgca atcacgagga gcccttgaag tcgtggagca cggcctcgtc gtcggcgacg
     1081 gtgtcgtcct tgaccgggaa tccgtcccga gcacagagtg aggaccatgg cctcggcaat
     1141 cgtcgacgcc caccaccact tctgggatcc caggaccgcg gactatccgt ggatgaccgg
     1201 tgcatacgag ccactacgga aggcctttgg accatccgat ctcgccccgc tactcgaggc
     1261 cgtcggggtg acggccacga tcgttgtgca ggcccgacag gagctcgacg agacgctttc
     1321 cctgctcgag atggcagaca tgacaccatg ggtggcaggg gtcgtgggat gggtcgacgt
     1381 cacccgggcc gatgtggggg agaccatcgc gaggatccgt gaaggagaac acgggaatcg
     1441 gttggtcggg atccggcatc tggttcacga cgagcccgac cccgaatggc tgcttcgcga
     1501 cgacgtccta cgaggactat ccgccgtggc cgaaaccgga ttggtctacg acctgctggt
     1561 gaggacccgc gagctgcctg cggccaccga ggcggccagg cgactccccg ggctccgctt
     1621 cgctctcgat catctggcca agcctccgat ctcgacgcgg cagatcgagc cgtgggcctc
     1681 cgccatcagc gagatcgcca gtctccccaa tgtgacctgc aaggtgtctg gcatggtcac
     1741 cgaagccgac tggtcgtcat ggcgttcagc cgacctgacg ccctacatcg tcaaagccgt
     1801 ggagatgttt ggcgccgatc gactgatgtg gggatccgac tggcccgtct gcacaatggc
     1861 cgcgacatac tccgaggtgt acgagaccgc agtcgagatt ctctcctccc aggtgggaaa
     1921 cgagctcgag tcgatcctcg ggaggtgtgc gtcctcgacc tatggtctcg agcccgaggg
     1981 cgtgcagcac actctccacc gggaagccgt cggactcaga ctcgacgtcg cctcagcgca
     2041 ccacacgggc accgtgagcg acgacggctc tcatcacttc cgagctcgtc accatcgaag
     2101 tgtcccccgg tgtcgtcatg gtcaaggctc catgggcggc accgatctcc actgcctccg
     2161 ccggcgacct tccttctaga agagcccaga tcatgccgct ggcgaagcca tcgcccccgc
     2221 caaccctgtc gtagatctcg aggtccgaac ggatccggga ctcgtggaat accccctcgg
     2281 cgtggaggat cgctcccccg tcgttgatcc cggcggtctt cgcgttccgg agggtggtcg
     2341 cgatcatccc
//
LOCUS       DATITW010000772         1604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_77960, whole genome shotgun sequence.
ACCESSION   DATITW010000772 DATITW010000000
VERSION     DATITW010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77960"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VLS86_10790"
     CDS             complement(<1..383)
                     /locus_tag="VLS86_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO51023.1"
                     /translation="MPRRIGDVILEVEDLHTYCFSRWGVVKAVDGVSFSLRQGEALGI
                     VGESGCGKTMTALSLLRLVPRPAARIVKGTIRLEGENLLDKSEREMRKIRGRRISMIL
                     QDPQTSLNPVFTVGNQLIEAIKIHHR"
     gene            complement(437..>1604)
                     /locus_tag="VLS86_10795"
     CDS             complement(437..>1604)
                     /locus_tag="VLS86_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSO51024.1"
                     /translation="HEVVFRFKRPTTVMPYAASRAGDLRIVSKAQWDKEGQEGFEKRP
                     AGTGSYRYVGRQPGLSVSFERADTHWKGEKPAFKELVFRLAREESTRLALLLSGEAHI
                     ADLPRELQKDALKRGMKIYSSSFPVDWMTVYLGGQYYMPGDPAFKPNVAWTKKPVRQA
                     LNMAINRKELLSTVFAGKGTLTYVTGWSPQSEGYNPEWKSRFDQMYGYNPTKAKALLK
                     DAGYGPGQLKLKVLAFTEPGESEGPQVAESLGIYYKDIGVETEIEVLDWAKVREMFRK
                     KAIQCCIWPNIISWRPSEEWIRTSYYSKSPNHHYENEFIDKHYDALTKALKPDERQRI
                     ARTIGDHLFEEFADIPLFWFANEVVANPKVVGEWVYPGLGAGRSTHFHLVKPGK"
BASE COUNT          243 a          532 c          515 g          314 t
ORIGIN      
        1 cggtggtgga tcttgatggc ctcgatgagc tggttgccga cggtgaacac ggggttcagg
       61 gaggtctgag gatcctgcag gatcatggag atgcgtcgcc cccggatctt gcgcatctcc
      121 cgctcgctct tgtcgaggag attctcgccc tcgagccgga tggtgccctt gacgatgcgg
      181 gcggccggcc gggggaccag cctcaacaac gacagcgcgg tcatggtctt cccgcaccct
      241 gactcgccca cgatgcccag agcttccccc tggcgtaagg aaaagctcac cccgtccacc
      301 gccttcacca caccccagcg gctgaagcag tacgtgtgga ggtcctcgac ctccaggatg
      361 acgtcgccga tccgtctggg catgcacgac ctgaccgcca ggggaggagg gccgtcccct
      421 ggcggccgcg gcggtcctat ttcccgggct tcacgagatg gaagtgcgtc gatctcccgg
      481 ccccgagccc ggggtacacc cactcgccca cgactttggg gttggcgacg acctcgttgg
      541 cgaaccagaa gagcgggatg tcggcgaatt cctcgaagag gtggtcaccg atcgtccgcg
      601 caatgcgctg ccgttcgtcg ggcttcagcg ccttggtcag ggcgtcgtag tgcttgtcga
      661 tgaactcgtt ctcgtagtgg tggttcgggc tcttgctgta gtagctcgtc cggatccact
      721 cctccgacgg cctccaggag atgatattgg gccagatgca gcactggatg gccttcttgc
      781 gaaacatctc ccgcaccttg gcccagtcga gcacctcgat ctccgtctcg accccgatgt
      841 ctttgtagta gatgccgagg gactcggcga cctgcgggcc ttccgactct ccgggctcgg
      901 tgaacgccag gaccttgagc ttgagttgcc cgggaccgta accggcgtcc ttcaagagcg
      961 ccttggcctt ggtcgggttg tagccgtaca tctggtcgaa tcgactcttc cactccgggt
     1021 tgtagccctc ggactgcggg gaccagccgg tcacgtacgt gagcgtccct ttgccggcga
     1081 acaccgtgct cagcagctct ttgcggttga tcgccatgtt gagcgcctgt cggaccggct
     1141 tcttcgtcca ggccacgttc ggcttgaagg ccgggtcccc gggcatgtag tactggccgc
     1201 ccaggtacac cgtcatccag tccaccggga acgacgacga gtagatcttc atgcccctct
     1261 tgagcgcgtc cttctgcagc tcgcgaggca ggtcggcgat gtgggcctcg cccgacagga
     1321 gcaatgccag ccgggtcgac tcctcgcggg ccagtcggaa caccagctcc ttgaacgccg
     1381 gcttctcgcc cttccagtgg gtgtccgccc gctcgaagct gacggagagg cccggctgcc
     1441 ggccgacgta gcggtaggag ccggtgccgg cgggccgctt ctcgaatccc tcctggccct
     1501 ccttgtccca ctgggccttg ctgacgatcc gcagatcgcc ggcccgggag gccgcgtagg
     1561 gcatgacggt cgtcgggcgc ttgaagcgga acacgacctc gtgg
//
LOCUS       DATITW010000773         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_719068, whole genome shotgun sequence.
ACCESSION   DATITW010000773 DATITW010000000
VERSION     DATITW010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719068"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VLS86_10800"
     CDS             complement(<1..827)
                     /locus_tag="VLS86_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51025.1"
                     /translation="MASRLDVSAVPLQGGYIAKQCPVVIQNQILVPEARTEPSPEIRH
                     RMDAGILFEAELFDRVRVEGPGDWVIIPSGPPQLAQELTVEALTRGALVIEQAFLPPD
                     ETGRRTGRPDLLVSHGGGYVPVDVKHHRTLDIAEGTVLVSDPPSPSPTDAFSLEGHSL
                     RTNSADALQLAHYRRMLEASGHASSSTLAGVLGKEAVIVWYDLDKPMWSTPAKSDGKK
                     RKLRTSGERYDFEFGFRLDIAATALTHLRSGDIALLVEPMACGECGGCGFHDHCFTTL
                     "
     gene            1018..1176
                     /locus_tag="VLS86_10805"
     CDS             1018..1176
                     /locus_tag="VLS86_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51026.1"
                     /translation="MPEWIGTIVFLSALGLSGAKIAGDFGKRGLLGYAMLLVMLVIYA
                     AAVAVAFA"
     gene            1218..1544
                     /locus_tag="VLS86_10810"
     CDS             1218..1544
                     /locus_tag="VLS86_10810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005272590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSO51027.1"
                     /translation="MEFRHDPDNHRYLAESDGETIGVAVYHLRNGRHIFVHTEVSPGH
                     EGEGVASDLARFALDDVRAGGGTVVPVCPFISTWVRRHPEYQDLVDQELLDRINGVQA
                     SRHADH"
     gene            complement(1561..1953)
                     /locus_tag="VLS86_10815"
     CDS             complement(1561..1953)
                     /locus_tag="VLS86_10815"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HSO51028.1"
                     /translation="MDPYRRKVRYWDVDANAHVFNTRYLVYVDDALTDLLDELGLGHG
                     SEVWDGYLMVLVHTEIDYRTEAVIGETLATTVGVERIGTSSITFGFRIVEEESGRIVA
                     AGKEVYVTVADGDRRPIRVPDQVRSRLG"
     gene            2030..>2805
                     /locus_tag="VLS86_10820"
     CDS             2030..>2805
                     /locus_tag="VLS86_10820"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HSO51029.1"
                     /translation="MRGLFATAELAPFVKVGGLGDAAAGLIRALRRRGVEIEVVLPDY
                     GWLPLDQDDEETLDVPDWAGPARVRRGSLDGFDNVSLIVTPTMARPHPYNDAFGRGWP
                     GNDTRFFSFAAAVAALAASSRPDVLHLNDWHTGAVLGFLPDPPPTVFTIHNLAYQGAA
                     DGAWLELIPHRAPAYEWYGGANPLTGAVALADRVVTVSPSFAAEALRPDTGFGAHEAL
                     AARGDAFSGILNGIDTEVWDPAVDPYLPVHFDGANPGRNR"
BASE COUNT          464 a          953 c          916 g          472 t
ORIGIN      
        1 agggtggtga agcagtggtc gtggaagcca cagcccccgc actcgccaca ggccatcggc
       61 tccaccagga gcgcgatgtc cccgcttcgg aggtgggtga gggcggtcgc cgcgatgtcg
      121 aggcggaacc cgaactcgaa gtcatacctc tcccccgagg tccgcagctt gcgcttcttc
      181 ccgtccgact tggccggcgt ggaccacatg ggcttgtcga ggtcgtacca gacgatcacc
      241 gcctccttgc ccagcacccc ggccagggtc gagctcgaag cgtgccccga cgcctccagc
      301 attcggcggt agtgggcgag ttggagagcg tcggcggagt tggttcggag cgagtgcccc
      361 tcgagagaaa aggcgtcggt ggggctgggg ctcggtgggt cggagacgag gacggtgccc
      421 tcggcgatgt cgagggtccg gtgatgcttg acgtcgaccg gcacataccc cccgccatgg
      481 gatacgagca ggtcaggccg gccggtcctc ctccccgtct cgtccggagg cagaaacgcc
      541 tgctcgataa ccaaggcgcc ccgggtcaac gcctcgaccg tcagctcctg ggccagctgg
      601 ggtgggccgg aagggatgat cacccagtcg ccggggccct cgacgcggac acgatcgaac
      661 agctcggctt cgaacaggat gccggcatcc atccggtgcc tgatctcggg agaaggctcg
      721 gtcctcgcct cggggaccag gatctggttc tggatgacga ccgggcattg ctttgcgatg
      781 tatccgccct gcagcggtac cgccgaaacg tcgagacggc tggccatgtc tgctgctcac
      841 ggtagatcac cgtctcggcc cgtttcgtgc gtgcaacctg acgcggggag gcaccgtcgt
      901 gttcgcctcc ctcccccgta attgggaccg agaggggtca aatacggacc tgtggctccc
      961 aattacgggg aggggggagt agattcggga tagcggagcg agtggagcgg aggtctcatg
     1021 cctgagtgga tcggaaccat cgtcttcctc agtgccctcg ggctgtcagg ggccaagatc
     1081 gcaggggatt ttggtaaacg gggcctgctc ggatacgcca tgctgctcgt gatgctggtc
     1141 atctacgccg cggccgtcgc cgtagccttc gcctaaccga cccggccggt gcccggctgc
     1201 cctaagttgg cgccgacatg gaattccgcc acgaccccga caaccaccgc tatctggccg
     1261 aatccgacgg cgagaccatc ggggtcgcgg tttaccacct acgcaacggg cgccacatct
     1321 tcgtccacac cgaggtgtcg ccgggacacg aaggagaggg agtcgcctcc gatctggcgc
     1381 gctttgcact cgacgatgtc agagcgggcg gaggaaccgt ggtaccggtg tgccccttca
     1441 tctccacctg ggtgagacgc caccccgagt accaggacct ggtcgatcag gagctgctcg
     1501 accggatcaa cggggtgcag gcctcgcgtc acgccgacca ctgagcgtgt tggatccgac
     1561 tcagccgagc cgcgacctga cctgatccgg gaccctgatc ggacgtcggt cgccgtcagc
     1621 caccgtcaca tagacctcct tgcccgcggc gactatccga cccgactcct cctcgacgat
     1681 gcgaaacccg aacgtgatgg acgaggtccc gatccgctcg acaccgaccg tcgtcgccag
     1741 cgtctccccg atcaccgcct cggtccggta gtcgatctcg gtgtggacca gcaccatgag
     1801 atagccgtcc cagacttccg agccatgacc gagcccgagc tcgtcgagaa ggtcggtgag
     1861 ggcgtcgtcg acgtacacca gatacctggt gttgaacaca tgggcgttgg catccacgtc
     1921 ccaatagcgg accttgcgcc ggtacggatc catggccgac aatctaggca ggagaccacc
     1981 cgcgagattc ccctgcatat acggtatctc tcctgctaga catcagccga tgcggggact
     2041 ctttgccaca gccgagctgg cgccattcgt caaggtgggt ggtctcgggg atgccgccgc
     2101 cggtctgatt cgagccttgc gccgtcgcgg ggtggagatc gaggtggttc tccccgacta
     2161 cggctggctg cccctggacc aggacgacga ggaaacgctc gacgtccccg actgggcggg
     2221 gccggcccgg gtgcggcggg gcagcctcga cgggttcgac aacgtctctt tgatcgttac
     2281 cccgaccatg gcccgacccc atccctacaa cgacgcgttc gggcggggct ggccgggcaa
     2341 cgacacccgc ttcttctcct tcgccgccgc ggtggccgcc ctcgccgcca gcagtcgccc
     2401 cgacgtgctc catctcaacg attggcatac gggagccgtc ctgggctttc tccccgaccc
     2461 accacccaca gtgttcacca tccataatct cgcctatcag ggagcagccg atggcgcctg
     2521 gctggagctg atcccccatc gcgccccggc ctacgagtgg tacggggggg ccaacccgct
     2581 gaccggggcc gtcgcccttg ccgacagggt ggtcacggtc agccccagct ttgccgccga
     2641 ggcgctgcgg cccgacaccg ggttcggggc ccacgaggcg ctggcggcgc ggggcgacgc
     2701 attctccggg atcctcaacg ggatcgacac cgaagtctgg gacccggcgg tcgaccccta
     2761 tctcccggtt catttcgacg gggccaaccc gggccggaat cgaga
//
LOCUS       DATITW010000774         1882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_558767, whole genome shotgun sequence.
ACCESSION   DATITW010000774 DATITW010000000
VERSION     DATITW010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558767"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VLS86_10825"
     CDS             complement(<1..417)
                     /locus_tag="VLS86_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HSO51030.1"
                     /translation="MNRDWVDKDFYQILGVGSESTPEEIKKAYRKLAQTYHPDANPGN
                     DEAEEKFKEISEAYATLSNVEQRKEYDQVRRLVGSGGYPGYGAGGGGFGGQQVRVEDL
                     SDLLGGLGGIGDIFGFGSGSRGGPARGADVLADLSIS"
     gene            complement(414..965)
                     /locus_tag="VLS86_10830"
     CDS             complement(414..965)
                     /locus_tag="VLS86_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HSO51031.1"
                     /translation="MSELEPEVVIGEIVEARAPGIDLPEDPDEAIGLLLSLLEESQQE
                     ATSYLDDLKRVAADFDNFRKRALREQSAVVDHAAERVTRALLPVLDSFDAALAVEVNT
                     DAERRLLSGMIGTREQLLNALAQEGLEVIPTVGEPFDPEIHEPAGAPEGDGPFVVAQE
                     LRRGYRLRGKLLRAALVALESER"
     gene            complement(958..>1882)
                     /locus_tag="VLS86_10835"
     CDS             complement(958..>1882)
                     /locus_tag="VLS86_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HSO51032.1"
                     /translation="GVKITDIDGVILVGGQTRMPLVQQRVKDFFGREPRKDVNPDEAV
                     AIGASIQAGVLKGEVKDILLLDVTPLTLGIETKGGVMTRMIERNTTIPTKRSEIFTTA
                     ENNQPEVEIHVLQGERTMANDNKSLGKFRLTGIPPAPMGVPQIEVAFDIDANGIVNVN
                     AKDLATGKEQAMTITGGSALAEGEIDRMIKDAEAYAEEDAKRREAVETRNQADQLVHQ
                     TDKLVEEQGEQMTEDEKAGIASALTALKEAMADESTETSEIRSRMDSLISASQAMATR
                     LYQQAGSTGEPGEATAEDDDVVEAEIIEDDE"
BASE COUNT          271 a          637 c          582 g          392 t
ORIGIN      
        1 gctgatgctg aggtcggcca ggacgtcggc gccgcgggcc gggccgcccc gggagcccga
       61 gccaaagccg aagatgtcac caatcccgcc caatcctccg aggaggtcgg agaggtcttc
      121 aacccgcacc tgctggccac cgaaaccgcc gccgccggcg ccgtatccgg ggtacccgcc
      181 cgagcccacc agacgccgca cctggtcgta ttccttgcgc tgctccacgt tggacaatgt
      241 ggcgtaggcc tccgagatct ccttgaactt ctcctcggcc tcgtcgttcc ccgggttggc
      301 gtcggggtga taggtctggg ccagcttgcg ataggccttc tttatctctt cgggagtcga
      361 ttcggagccg acaccgagga tctggtagaa gtccttgtcg acccagtccc ggttcatcgt
      421 tccgattcca gggccaccag ggcggcgcgc agcagtttcc ctctgagccg gtagcccctc
      481 cgcagctcct gggccaccac gaatgggccg tcgccctcgg gtgccccggc aggctcgtgg
      541 atctccggat cgaacggctc cccaacggtc ggaatgacct cgagcccttc ctgggccaga
      601 gcgttgagga gttgctctcg ggtcccgatc atcccgctca gcagacgccg ctcggcatca
      661 gtgttgactt cgacggccag ggcggcgtcg aacgagtcga gcaccggcaa cagggctcgg
      721 gtaacgcgct cggcggcatg gtcgaccacg gccgactgct cgcggagcgc ccgcttgcga
      781 aaattgtcga aatcggcggc gacccgtttc aggtcatcga gatatgaggt tgcctcctgc
      841 tgtgactctt cgagcagcga cagcagcagc ccgatggcct catccgggtc ctcggggaga
      901 tcgattcccg gtgctcgcgc ctcgacgatc tccccgatga cgacttccgg ctctaactca
      961 ctcatcgtcc tcaatgatct ccgcttcgac cacgtcgtca tcctcggctg tggcctcccc
     1021 gggctcaccg gtggagccgg cctgttggta gagacgcgtc gccatggcct ggctcgccga
     1081 gatcaggctg tccatgcggg accgaatctc ggaggtctcc gtcgattcgt cggccatggc
     1141 ttccttgagg gccgtcagtg cggatgcgat tccggccttc tcgtcttcgg tcatctgctc
     1201 gccctgctcc tcgacgagct tgtccgtctg atgcacgagc tggtcggcct gattgcgggt
     1261 ttcaaccgcc tctcggcgct tggcgtcctc ttcggcgtag gcctcagcgt ccttgatcat
     1321 tcgatcgatc tcgccttccg ccagtgccga tcctccggtg atcgtcatcg cctgctcctt
     1381 gcccgtggcc aggtccttgg cgttcacatt cacgatccca ttggcgtcga tgtcgaaggc
     1441 aacctcgatt tgagggaccc ccatgggagc cggcgggatc ccggtcagcc ggaacttgcc
     1501 caggctcttg ttgtcattgg ccatggtgcg ctcgccctgg aggacgtgga tctcgacctc
     1561 gggctggttg ttctcggcag tggtgaagat ctccgatcgc ttggtgggga tcgtcgtgtt
     1621 gcgctcgatc atccgtgtca tgaccccacc cttggtctcg atgccgaggg tgagtggcgt
     1681 gacgtcgagg aggaggatgt ccttgacttc gcccttcaag acaccggcct ggatcgacgc
     1741 gccgatcgcg acggcttcgt cgggattcac gtccttgcgc ggctcgcggc cgaagaagtc
     1801 cttcacgcgt tgctgcacga gcggcatgcg cgtctgcccc ccgacgagga tcaccccgtc
     1861 gatgtcggtg atcttcacac ca
//
LOCUS       DATITW010000775         1671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_277908, whole genome shotgun sequence.
ACCESSION   DATITW010000775 DATITW010000000
VERSION     DATITW010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277908"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VLS86_10840"
     CDS             complement(<1..1305)
                     /locus_tag="VLS86_10840"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HSO51033.1"
                     /translation="MNYTEALAYLDRHIALGVKPGLDRMRGLLEAMGRPDEGYPIIHV
                     AGTNGKTSTSRLATLLLVAHGLTTGTYISPHLQRVEERLAVNGRIATEEEFALAVSDV
                     EAFAVVREQQGHDANTYFELTTAAAFAFFANQPVQAAVLEVGLGGRLDATNVVDAEVC
                     VMTSIGIEHTEYLGPDLASIAGEKAAIAGLNSILVTGPLPEPALEVAEGRARELGIHH
                     RHYGKDFSVLAADRGVGGWLVSIDGAEDTYEDLFLPLHGRHQVVNLATAIAAVEALLG
                     RKLDAEAVHDAAASATMPGRMETLGAEPLLMLDGAHNTDGVDVLVESLFEEFPSKSWQ
                     VVFGVMGDKNVEAMAERLADVADGFVVTAPESERSVTPDELAIRVAAVTGLPVREVGS
                     VEDAVEIARRLAGPEGAVLVTGSLYLVGEVRSFLTGGTGLRGG"
     gene            complement(1370..>1671)
                     /locus_tag="VLS86_10845"
     CDS             complement(1370..>1671)
                     /locus_tag="VLS86_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51034.1"
                     /translation="YVLNGVNVSGQTLAGPISGTVLVEVQPGYVLSNGAPTQMTFSVA
                     AADVCDEVLAEVIDPDEVAALPLTGIDSKALLGVSILLLGAGIYLVHVAQRGEEG"
BASE COUNT          294 a          580 c          508 g          289 t
ORIGIN      
        1 accgccgcgc aaccctgtcc cgccggtaag gaagcttcgt acctcgccga ccagatacag
       61 agagccggtc accaggaccg ccccctccgg cccggccagc cttctcgcga tctccaccgc
      121 atcctcgact gaccccacct cccgcaccgg gagaccggtg accgcggcga ccctgatcgc
      181 cagctcatcg ggagtgaccg accgctccga ttcgggcgcg gtcacgacga acccgtcggc
      241 gacgtcggcc aggcgctccg ccattgcctc cacgttcttg tcacccatca ccccgaacac
      301 cacttgccag ctcttggatg ggaactcctc gaagagcgac tccacgagga cgtccacccc
      361 gtcggtgttg tgcgctccgt ccagcatcag caagggctcg gcgcccagtg tctccatccg
      421 accgggcatg gtcgcggacg ccgccgcgtc gtgaaccgcc tcggcatcca gcttcctccc
      481 cagcaacgcc tcgacggctg ctatcgcagt cgcgaggttg accacctgat ggcggccgtg
      541 gaggggaaga aacaggtcct cgtaggtgtc ctcggctccg tcgatgctca ccaaccaccc
      601 gccaactccc cgatcggcgg caagaacgga gaagtccttc ccgtagtgac gatgatgaat
      661 ccccagttcg cgggcccgtc cctccgccac ctccaatgcc ggttccggca atggcccggt
      721 gaccaggatc gagttgagac cggcgatcgc cgccttctcc cctgcgatgc tggccagatc
      781 cgggcctagg tactcggtgt gctcgatacc gatgctggtc atgacacaga cctcggcgtc
      841 aacgacgttg gtggcgtcga ggcgtccccc gagcccgacc tccagcaccg cggcctgcac
      901 cggttggttg gcaaagaacg cgaacgccgc ggcggtggtc aattcgaagt aggtgttggc
      961 gtcatgacct tgttgttccc ggacgacggc gaacgcctcg acgtccgaga cggcaagcgc
     1021 gaactcttcc tcggtcgcga ttcgcccgtt cacggccaac ctctcctcga cacgctgaag
     1081 atgcggggag atgtaggtcc cagtggtcag tccgtgagct acgaggagga gcgtggccag
     1141 acgtgacgtc gaggtcttcc cgttggtgcc tgcgacatgg attatgggat acccctcgtc
     1201 gggtcggccc attgcctcga gcagcccgcg catgcgatcg aggccaggct tcaccccaag
     1261 ggcgatgtgg cgatcgaggt aggcgagagc ttcggtgtag ttcatgcaag aggaagggcc
     1321 tcgggaggtt aatcccgagg cccttccccc tctcagaagg ccggggcgat cagccttcct
     1381 ccccccgctg ggcgacgtgg accaagtaga tccccgctcc cagcagcagg atcgagacgc
     1441 cgaggagggc tttactgtcg attcctgtca acggaagggc tgcgacctcg tccgggtcga
     1501 tcacctcagc caacacctcg tcacaaacgt cagctgcagc gacactgaat gtcatctgtg
     1561 tcggcgctcc gttggatagg acgtagccgg gctgtacctc gacgaggacg gttccggaga
     1621 tcgggccggc gagggtttga ccgctcacgt tgaccccgtt gaggacatat c
//
LOCUS       DATITW010000776         1711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_798412, whole genome shotgun sequence.
ACCESSION   DATITW010000776 DATITW010000000
VERSION     DATITW010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798412"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1711)
                     /gene="carB"
                     /locus_tag="VLS86_10850"
     CDS             complement(<1..>1711)
                     /gene="carB"
                     /locus_tag="VLS86_10850"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HSO51035.1"
                     /translation="VLWGLAQSPRGEVLVEQSVLGWKEYELEIMRDRKDNAVIVCSIE
                     NLDPMGVHTGDSITVAPIQTLSDREYQEMRDDGIAVLRAIGVETGGSNVQFAVDPETG
                     RRLVIEMNPRVSRSSALASKATGFPIAKIAALLAVGFTLDEIANDITGATPASFEPAL
                     DYVVVKIPRFDFAKFPSASTLLNTSMRSVGEAMAIGRTFPEALQKALRSMENGRFGLN
                     ADPAEQAILGMSDDDLASSVEEPRPARIFAVGEALRRGWGVDRVSALSSIDPWFVDQI
                     AEIVELRTEIETVGPSDVELMRQAKRFGYSDRQLAHLWGGTEQEVRAQRLAAGIVRSY
                     KTVDTCAAEFEAFTPYLYGTFEEESEVPPAQRPRVVVLGSGPNRIGQGIEFDYCCVHA
                     SFALRDAGYETVMINCNPETVSTDYDTSDRLYFEPLTIEDVLAVIDAEQPEAVIVQLG
                     GQTPLNLAKALHDAGVHIAGTSPASIALAEDREQFGELCRSLGLRQPAAGSATSATEA
                     EDVVDQVGLPVMVRPSFVLGGRMMRVIFSVEELAAYVREIYGEANPELTGAPILIDRF
                     LEAA"
BASE COUNT          283 a          588 c          544 g          296 t
ORIGIN      
        1 cgcggcctcg agaaaccggt cgatgaggat cggcgcaccg gtcagctcgg ggttcgcctc
       61 cccatagatc tccctgacat acgcagccag ctcctcgacg ctgaagatga ccctcatcat
      121 ccggcccccg agcacgaacg acggccggac catcaccggc aaacccacct ggtcgaccac
      181 gtcctcggct tccgtcgccg aagtggcaga gccggctgcg ggttgtcgaa gacccaggct
      241 tcgacacagc tcgccaaact gctcccgatc ctcggcgagc gcaatcgagg cgggagaggt
      301 cccggcgatg tgaacgccgg cgtcgtgcaa agccttggcc aggttgagcg gggtctggcc
      361 gccgagctgg acgatgaccg cctcgggctg ctcggcgtcg atcacggcca gcacgtcctc
      421 gatggtgagt ggctcgaagt agagccggtc cgaggtgtcg tagtcggtgg acaccgtctc
      481 cgggttgcag ttgatcatga ccgtctcata gccggcatcc cgaagcgcaa aggacgcgtg
      541 cacgcagcag tagtcgaact cgatgccctg tccgatgcgg ttcgggcccg atccgagcac
      601 caccaccctc ggccgttgcg cagggggcac ctccgactcc tcctcgaacg tcccgtacag
      661 gtatggggtg aacgcctcga actcggcggc acaagtgtcc acggtcttgt agctgcggac
      721 gatccccgcg gcgagacgct gcgccctgac ttcctgctcc gtgccgcccc agaggtgggc
      781 gagttggcga tcggagtacc cgaaccgctt cgcctgtcgc atcagctcga catctgaggg
      841 accgacggtc tcgatctcgg tgcgcagttc gacgatctcg gcgatctgat cgacgaacca
      901 cgggtcgatc gagctcagcg ccgacacccg gtccacgccc cagcctcgac gcaatgcttc
      961 acccactgcg aagatccggg cgggacgggg ctcttcgacg gagctggcca ggtcgtcgtc
     1021 gctcatcccc aggatggcct gctcggccgg atcggcgttc agcccgaacc tgccgttctc
     1081 catgctgcgc agggccttct gcagcgcctc gggaaaggtc cggccgatcg ccatcgcctc
     1141 tccaacgctt ctcatcgatg tgttgagcag cgtcgaggcc gaggggaact tggcgaagtc
     1201 gaatcgtggg atcttgacca ccacgtaatc cagcgcaggc tcgaacgagg ccggtgtggc
     1261 cccggtgatg tcgttggcaa tctcgtcgag agtgaagccc accgcgagga gcgccgcgat
     1321 cttggcgatg gggaagccgg tggctttcga ggccagcgcc gatgaccggc tcaccctggg
     1381 gttcatctcg atgaccagtc tccggccggt ctcggggtcc acggcgaact ggacgttcga
     1441 tcccccggtt tccacgccga tggccctgag caccgcgatc ccgtcgtcgc gcatctcctg
     1501 gtactcgcgg tcggagaggg tctggatcgg ggcaaccgtg atcgagtccc cggtgtgcac
     1561 ccccatcggg tccaggttct cgatcgagca gacgatgacc gcgttgtcct tgcggtcgcg
     1621 catgatctcg agctcgtatt ccttccagcc gagcaccgac tgttcgacca gcacctcgcc
     1681 gcgcgggctc tgcgccaggc cccacagcac c
//
LOCUS       DATITW010000777         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_558912, whole genome shotgun sequence.
ACCESSION   DATITW010000777 DATITW010000000
VERSION     DATITW010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558912"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..841
                     /locus_tag="VLS86_10855"
     CDS             146..841
                     /locus_tag="VLS86_10855"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSO51036.1"
                     /translation="MAETESTTVTDRAYAQLRELLTSDSVSPGDHLKEVALAEWLGIS
                     RTPVRAALQRLELDQLVRRSHNSGYSVDGITPTDVREVCDVLRLVDTAVFIRASENLT
                     EEQGVALRECVDMMEEAAAEKDLDRWSEGDGVFHEILQKASNHALLSDIAAKQRRRLQ
                     RFRTSDPGRLERLALCAEEHASIAGSVAATDHEATRRLVHDHIDHMEASLIRQLDVAR
                     PFISTNSSALDHV"
     gene            867..>1557
                     /locus_tag="VLS86_10860"
     CDS             867..>1557
                     /locus_tag="VLS86_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSO51037.1"
                     /translation="MLQHELSDVVGAKHTSIDAGELSEQAADWSWMSQLYTLHGMQLP
                     VADVMVRPGTAQEVVSVLRIASDHRVPVVPRGGGSGTQGGTFALYGGISIDLTRMDRI
                     IEVDETSLVITAEAGVTGPQLEQALSPLGLSVAHEPGSFHFGATVGGWLAARGSGVRS
                     TKYGKAEDLVLQAEVALPPGRLVSTLPVPSHAAGPGLLPLLVGSEGTMGVLTAATLRM
                     DPIPETQAFLTF"
BASE COUNT          278 a          498 c          509 g          272 t
ORIGIN      
        1 tcatcggcaa cccgatgcgt gccaccaagg agaaaggcga ggagtcgttc aggagatact
       61 ccgagcacgt ggctcgaggt gtgctcgagc tcatgcaggt gccggtcaag gtcgagaacc
      121 gagagttcgt cgaccgggtc atgtgatggc agaaaccgag tcgaccaccg tcaccgaccg
      181 ggcatatgcc cagctgcgcg agctcctcac cagtgacagt gtgagcccgg gtgaccacct
      241 caaagaggtg gcgctggcgg agtggctcgg aatcagtcga acaccggtgc gggcggcttt
      301 gcaacggttg gagcttgacc agctggtgcg tcgaagtcac aacagcgggt attcggtcga
      361 cgggatcacc ccgaccgatg tgcgggaggt gtgtgacgtg ctccgcctcg tcgacaccgc
      421 tgtgttcatt cgagcatcgg agaatctgac cgaggaacaa ggtgtggcac tacgcgagtg
      481 tgtcgacatg atggaggagg cggcggcaga aaaagatctg gaccgatggt cagaaggcga
      541 cggtgtcttc cacgagatcc tgcagaaggc ttccaatcac gccttgctct cggacatcgc
      601 ggccaagcag cggcgcaggc tccagcggtt caggacgtcc gaccccgggc gcctcgagcg
      661 gctggctctt tgcgccgagg agcacgcctc gatcgccggc tcggtcgccg ccacggacca
      721 tgaggcgaca cggcggctcg tccacgacca catcgatcac atggaggcca gcctgattcg
      781 ccagctcgac gtggcgcgtc cattcatctc gacgaacagc tccgccctcg atcatgtctg
      841 acgcccggtc cgagtatcga ctgaggatgc tccaacacga gctgtccgac gtcgtcggcg
      901 ccaaacacac ctcgattgac gcaggtgagc tctccgagca ggccgcggac tggtcctgga
      961 tgtctcagct ctacacgctt cacgggatgc agctacctgt tgccgatgtc atggttcgtc
     1021 ccggcaccgc ccaggaggtc gtcagtgtgt tgcgcatagc cagcgaccat cgggtcccgg
     1081 tggtgccgcg gggcggtggt tccggcacac agggtgggac cttcgccctg tatggcggga
     1141 tctccatcga cctgacccga atggaccgga tcatcgaagt cgacgagacg agcctcgtga
     1201 tcaccgccga ggcaggcgtc accgggccac aactggaaca ggccctgtcc cctctcgggc
     1261 tgtccgtcgc ccacgagccg gggtcttttc acttcggagc caccgtgggc ggttggctgg
     1321 cggctcgcgg gtccggggtt aggtcaacga agtacggcaa ggccgaagat ttggtcctcc
     1381 aagccgaggt cgccctcccc ccggggcggt tggtctcgac gctcccggtc ccgagccatg
     1441 ccgccggacc ggggctcctc cccctcctcg tcggctcgga aggcacgatg ggtgttctga
     1501 ccgctgccac gcttcgcatg gacccgattc cggaaacgca ggcctttctc accttcg
//
LOCUS       DATITW010000778         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_157779, whole genome shotgun sequence.
ACCESSION   DATITW010000778 DATITW010000000
VERSION     DATITW010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157779"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VLS86_10865"
     CDS             complement(<1..374)
                     /locus_tag="VLS86_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HSO51038.1"
                     /translation="MSGPGDARSVAAEVVGRVLRSGAYSNVLAEKATGQLSAQDRAKA
                     KALIFGVLRRLETIDRAIETGSRRRIDELDAEVVDRLRVACFEILYGELPIPIAVSAG
                     VDLVREGAPRAAGLANATLRRIS"
     gene            complement(371..1285)
                     /gene="fmt"
                     /locus_tag="VLS86_10870"
     CDS             complement(371..1285)
                     /gene="fmt"
                     /locus_tag="VLS86_10870"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HSO51039.1"
                     /translation="MSRIAFLGTPGAAVPTLRALAGSHDVVVVITQPDRPRGRSKSPA
                     PSPVKEEAADLGLDVRQPATGDEIRHVLDEAGPLDLGVVVAYGRLLPPEALGVPRNGM
                     LNVHFSLLPRWRGAAPVARALLAGDSMTGVTIIRLDHGLDTGPVLTAQAVDVQSEETS
                     GELTIRLAGLGARLLTEVIPPYLRGEMEPVTQTDEGMTYAAKIEPGDRPLQPQSPARE
                     AVNRVRALSPEPGATLAIDGETIQILRARLAGSSPGIGIWASQSDQPIAGFVDGGVEL
                     VELKPPGKAAMSGEAWLRGRNRAAGKIG"
     gene            complement(1282..1791)
                     /gene="def"
                     /locus_tag="VLS86_10875"
     CDS             complement(1282..1791)
                     /gene="def"
                     /locus_tag="VLS86_10875"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HSO51040.1"
                     /translation="MAIYPIRTYGDPVLRLETKPVVYVDDVVRTLAQDMIETMYEAPG
                     VGLAANQIGITRKIAVFDAQDGLGPRVMLNPEIVEVSGEWEYEEGCLSVPGHWWLIKR
                     PGFARVRALDLDGNEVEYAGDGLMGRVLQHEVDHLNKKLLIDNLDKSTRKKALRDLRD
                     EALGLATFE"
     gene            complement(1846..3588)
                     /locus_tag="VLS86_10880"
     CDS             complement(1846..3588)
                     /locus_tag="VLS86_10880"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51041.1"
                     /translation="MATFAVDNGFWYSIPDRFGDSIPVGSIVRVPLSGRKVRGFVVEI
                     DGDREGQLKEISALSGELPVFGPDLLKTLRWAATHYVAPLSVLLERAAPPNLPTSGDP
                     AVVSIGIEPDGGHPLHEIALMVARGQKRPTTALIGRWQSLDWLGSVSPILGSGASVLV
                     VTGTEAEASSIAEAASARGIVPVVVAGEVARDLTRAWVEAQQPGRLVVGTPRVASWQI
                     GGLALAIVLEEGRRAMKDRQTPTIQVRDLMTTRSRIEGFSLVFYGPTPSVEVLAAGAE
                     VVRHGSRAWPLVEVVDRRDDPPGAGLLAERTVAAIRAMRDEGRRVFVFTHRRNSDASM
                     RCVGCRRVRTCAGCGSRIGREQSCRRCGRESGPCAGCGGTVFEEMGSEPQRLVVEVNR
                     RLGEDLAGLHPTHRLVTVGTERDLVTLESVPLVVAVDVDGLMLGHNYRTSEEALRILA
                     RLANVVEPGHGRRMMAQTSLPDAPLVVALRRGEPVEYLEHLLGERARLGFPPASEMLA
                     VEIREASDPSGFDRELRELGGASVLGPAPAAPGWRWLIQGSLGRFKVALRPLAQRWRE
                     SGATVRFDADPIDL"
BASE COUNT          601 a         1357 c         1199 g          553 t
ORIGIN      
        1 gagatgcgcc tcagggtcgc attggccaat ccggcggctc gcggagcacc ttcccgaacc
       61 agatcgacac cggcgctgac cgcgatcggg atcggcagct ccccgtagag gatctcgaag
      121 cacgccaccc ggagacggtc gaccacttcg gcgtcgagct cgtcgatcct gcgtcgcgag
      181 ccggtctcga tcgcgcggtc gatggtctcg aggcgccgga gcacaccaaa gatgagcgcc
      241 ttggccttgg cgcgatcctg ggccgacagc tgcccggtgg ccttctccgc cagcacgttc
      301 gagtaggccc cggagcggag gacccgcccc accacctcgg cggcgacgga gcgggcgtca
      361 cccgggccgc tcaaccgatc ttccccgcgg cccggttccg tcctcgaagc cacgcctcgc
      421 cgctcatcgc cgccttgccc ggcggcttga gctcgacgag ttcgacgccg ccgtcgacga
      481 aaccggcgat cggctggtcg ctctggctcg cccagatccc gatcccgggc gaggagccgg
      541 cgagacgagc gcggaggatc tgaatcgtct cgccatctat tgccagcgtc gcccccggct
      601 ccggggagag ggctcgcacc cgattcaccg cctcccgggc cgggctctga ggttggaggg
      661 gccggtcacc gggctcgatc ttggccgcat aggtcattcc ctcgtcggtc tgggtgaccg
      721 gctccatctc accccgcagg tagggcggga tgacttcggt cagcaatcgg gcgcccaacc
      781 cggcgagcct gatcgtcaac tcccccgagg tctcctccga ttgcacgtcg acggcctggg
      841 cggtcagcac cggtccggta tcgaggccgt ggtcgagcct gatgatggtg acaccggtca
      901 tcgagtcccc cgccagcaag gcccgggcca caggggcggc tcccctccat cgcggaagca
      961 gtgagaagtg cacgttcagc atgccgttac ggggaacacc caaggcctcg ggcgggagca
     1021 ggcggccgta ggccacgacc accccgaggt cgaggggccc ggcttcgtcc aggacgtgtc
     1081 tgatctcgtc accggtggcc ggttgacgca cgtcgagccc cagatcggcc gcttcctcct
     1141 tgaccgggga gggggccggg ctcttggagc gaccgcgggg gcggtcaggc tgggttatca
     1201 ccaccaccac gtcgtggctg cctgccagcg cccggagagt tggcacggcc gctcccgggg
     1261 tgcccaggaa cgcgatgcga ctcattcgaa ggtggccaac cccagcgcct cgtcccgcag
     1321 gtcccgcaga gccttcttgc gcgtcgactt gtcgagattg tcgatcagga gcttcttgtt
     1381 cagatggtcc acctcatgct ggaggacacg gcccatgagc ccgtcgcccg cgtattcgac
     1441 ctcgttcccg tccaggtcga gtgccctgac ccgggcgaac ccgggccgct tgatcagcca
     1501 ccagtgcccg ggcacggaga gacagccctc ctcgtactcc cactccccgc tcacctcgac
     1561 gatctcgggg ttgagcatca ctcgcggccc caatccgtcc tgggcatcga agaccgcgat
     1621 cttcctggtg attccgatct gattcgcggc cagacccacc cccggcgcct cgtacatggt
     1681 ctcgatcatg tcctgggcca gggtccgcac cacgtcatcc acatagacga ccggcttggt
     1741 ctcgagacga agcaccgggt cgccataggt tcggatcggg tagatggcca tgatctcggg
     1801 ttgggggcgc cgtgagctta aaggctcacc gggggttggg agaggtcaga gatcgatggg
     1861 gtcggcatcg aagcggacag tcgcccccga ctctctccac cgctgcgcca gagggcgaag
     1921 agcgaccttg aaccgcccca gcgagccctg gatcaaccac cgccagcccg gggcggccgg
     1981 agccggcccg agcacactcg ccccaccgag ctcccgcagc tcgcggtcga atccgctggg
     2041 gtcactcgct tctcgtatct ccaccgcaag catctctgat gccgggggaa acccgagcct
     2101 ggcccgctcc ccgagcagat gctcgagata ctccaccggc tcgccgcgac gaagcgccac
     2161 caccaacgga gcgtccggca gcgaggtctg ggccatcatc cgtctcccgt gcccgggctc
     2221 gacgacgttg gccagcctgg cgaggatgcg aagcgcctct tccgatgtcc ggtagttgtg
     2281 ccccaacatc agcccgtcca catcgactgc caccaccagc gggaccgatt ccagcgtgac
     2341 gaggtcccgc tcggtgccca cggtgaccag acgatgggtg gggtgaagcc ctgcgagatc
     2401 ctcgccaaga cgccggttca cctccacgac cagacgctgc ggctccgatc ccatctcttc
     2461 gaacaccgtt ccgccgcacc ccgcacacgg ccccgattcc cgtccacagc gtcggcatga
     2521 ttgctccctg ccgatccggg agccacaacc ggcacaggtc cgcaccctcc tgcagcccac
     2581 acaccgcatc gaggcatcag agttgcggcg gtgggtgaag acgaacaccc ggcgtccctc
     2641 gtctcgcatg gcgcggatgg cagccacggt gcgctcggcg aggagcccgg cgccgggtgg
     2701 gtcgtcgcga cgatcgacca cctccaccag cggccaggcg cgggatccgt gacgcaccac
     2761 ctcggcgccc gcggccagca cctccaccga cggagtcggg ccgtagaaga caaggctgaa
     2821 tccttcgatt ctcgacctcg tcgtcatcag gtcgcggacc tggatcgtcg gcgtctgccg
     2881 gtccttcatc gcccgccgcc cctcttcgag gactatggcg agggccagcc caccgatctg
     2941 ccaggaggcg acccgcggcg tccccacgac gagtctcccc ggctgctgcg cctcgaccca
     3001 tgccctggtc aggtcccggg ccacctcgcc ggccacaacc acggggacga tgccccgggc
     3061 cgatgccgcc tcggcgatcg acgaagcttc ggcctcagtc ccggtcacga ccagcaccga
     3121 tgcccctgac ccgaggatcg gggacaccga ccccagccag tcgagcgact gccagcggcc
     3181 gatcagggca gtcgtcggtc gcttctgtcc ccgggcgacc atgagcgcga tctcgtgcag
     3241 cggatggccc ccatccggtt cgattccgat cgagacgacg gctggatcgc cggacgtggg
     3301 cagattcggg ggggcggccc gctccaggag cacggacagt ggggccacgt agtgggtcgc
     3361 cgcccaacgc agggtcttga gaagatcggg gccgaacacc ggcagctccc cggagagggc
     3421 cgagatctcc ttgagctggc cttcccgatc accgtcgatc tccacgacga agccgcggac
     3481 cttgcgcccg gagagaggaa ccctgacgat ggagccgacc gggatcgagt ccccgaagcg
     3541 gtcggggatc gaataccaga acccgttgtc aacagcgaac gtcgccacgc tggggacgac
     3601 gcgggccgtg ggtcgactca gaggtcggcc gcgccctgga tcgcctcgac catgtccaga
     3661 cgcgcccagg cgaagccggg ccggccaaag tggccgtaag cggcggtacc
//
LOCUS       DATITW010000779         1669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_358093, whole genome shotgun sequence.
ACCESSION   DATITW010000779 DATITW010000000
VERSION     DATITW010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358093"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VLS86_10885"
     CDS             complement(<1..981)
                     /locus_tag="VLS86_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HSO51042.1"
                     /translation="MAGDYYEILGVGRDATSEEIKKAFRRIARATHPDANPGDPTANA
                     NFRQAAEAYEVLSDPDRRRRYDRGDTIDLSDLFSGWGGLDDLLRSVFGEGSGFAGAGN
                     PSRPTRGRDILVRAQVDLAGAAFGTEATIQFRSRATCQECSGTGAQGEGGLTPCPECH
                     GNGSVRMARRSLFGTMMTVGTCPTCRGEGVLITEPCPACRGQGSLDEDTSMTVEIPAG
                     VSSGTRLRLSGRGESGGRNGPRGDLYVEVQVDPDPRFERQDSELVHRVTLGIAESTLG
                     TRLSVPLLEGGDIDLDIPAGTQPGAVFRLPGLGVTRLGRRARGDLHVVVAV"
     gene            complement(984..>1669)
                     /locus_tag="VLS86_10890"
     CDS             complement(984..>1669)
                     /locus_tag="VLS86_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HSO51043.1"
                     /translation="KATSDLLAEVTDLPAVVVAPGLAGDRVSALHSVQVTADQVLLIV
                     VTEGGRVLQQRARVRVPISPGELETAHRLVSAAAVGMDLGSDLSISDEEMSSLAEPAR
                     EAVLTINDGLHLAGAAGTEMFIGGTQRLAAVWEDLSAVQRILEVLEREAEVMKILAGA
                     SGTSIQIGSELPVLDVDMAVVSTTYEAGSVGGSVGVIGPMRMNYKQAISAVEEVSREL
                     EDRISSNQD"
BASE COUNT          264 a          611 c          531 g          263 t
ORIGIN      
        1 caccgcgacc accacatgga ggtctcccct cgcccggcgg ccgagacggg tcactcccag
       61 ccccggcagc cggaacaccg cccctggctg ggtgccggcg gggatgtcga ggtcgatgtc
      121 gcccccttcc agcaagggga ccgagagccg ggtgccgagc gtcgactcgg cgatccccag
      181 agtcacgcga tgaaccaact cggagtcctg tctctcgaat cgcgggtccg gatccacctg
      241 cacctcgacg tagaggtcgc cccgggggcc gttcctccct cccgactcac cccgaccgga
      301 gagcctgaga cgggtcccgg acgatacgcc ggccgggatc tcgaccgtca tcgacgtgtc
      361 ctcgtcgaga gagccctggc cgcgacaggc cgggcaaggc tcggttatga ggaccccctc
      421 accacgacag gtggggcagg tcccgaccgt catcatcgtc ccgaacagtg agcgtcttgc
      481 catccgaacc gagccgttgc cgtgacattc cgggcagggg gtcagaccac cctctccttg
      541 ggcgccggtg cccgagcact cctggcaggt ggccctggag cgaaactgga tggtcgcctc
      601 ggtgccaaag gcggctccgg ccagatcgac ctgggcccgg accaggatgt cccgaccccg
      661 ggtggggcgg ctgggattgc ccgcccccgc aaaaccggac ccctcgccga acaccgagcg
      721 gagtaggtcg tcgagaccgc cccagcccga gaacagatcc gacaggtcga tcgtgtcgcc
      781 ccggtcgtac cggcgtcgcc gatcgggatc ggagaggacc tcataggcct cggcggcctg
      841 gcggaagttg gcgttggcgg tcgggtcgcc agggttggca tcggggtgcg tggcccgagc
      901 gattcgccgg aaggccttct tgatctcctc gctggttgca tcacggccga cgcccaggat
      961 ctcgtagtag tccccggcca tgctcaatcc tgattggagc tgatccggtc ctccaactcc
     1021 cgggagacct cctcgactgc agatatcgcc tgcttgtagt tcatgcgcat cgggccgatg
     1081 acccccaccg agcctccgac cgagcctgcc tcgtaggtcg tggacacgac cgccatgtcc
     1141 acgtcgagga ccggcaactc ggagccgatc tgaatcgagg tgcccgaagc gccggcgagg
     1201 atcttcatca cctcggcctc tcgttccagg acttcgagga tccgctgcac tgcgctcagg
     1261 tcctcccaca ccgccgccag ccgctgcgtg cccccgatga acatctccgt ccctgccgcc
     1321 ccggcgagat gcagaccgtc attgatggtg agcaccgcct cccgtgccgg ctcggcgagg
     1381 ctgctcatct cctcgtcgct gatcgagagg tcactgccga gatccatccc caccgcggcg
     1441 gcactgacca gccgatgggc ggtttccagc tccccgggcg agatcggcac cctcaccctg
     1501 gctctttgct ggaggacacg cccgccttcg gtgaccacga tcagaagcac ctggtcggct
     1561 gtcacctgga cactgtgcag ggcgctcacc cggtccccgg cgagacccgg cgccaccacc
     1621 acggcgggga ggtcggtcac ctcggccagg aggtccgatg tcgccttga
//
LOCUS       DATITW010000780         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_678334, whole genome shotgun sequence.
ACCESSION   DATITW010000780 DATITW010000000
VERSION     DATITW010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678334"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1185
                     /locus_tag="VLS86_10895"
     CDS             79..1185
                     /locus_tag="VLS86_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSO51044.1"
                     /translation="MAAAVSLDNVSKLFGEVTAVDGVSLDIADGEFFSMLGPSGSGKT
                     TCLRMIAGFEVPSDGRVLLHGSDVSDLPPYDRDVNTVFQDYALFPHMTVEQNVGYGPM
                     VRKVDDAERAQRVSGALAMVHLTEFAQRKPSQLSGGQRQRVALARALINRPSVLLLDE
                     PLGALDLKLRQAMQLELKTLQREVGITFIYVTHDQEEALTMSDRLAVFNHGKVEQVGT
                     PTEVYENPRNAFVAGFVGTSNVISGPLAQRIVGSSKPFTVRPEKITIQEPASPVIDGR
                     TAVDGTVAEVVYLGMYTRYRVDIGGAVIEVAAQNTAAEHDDAQSQVGRQVRLTWIEDS
                     CRPLEESPREVGANEKAPALAGAGNNVPEEEILE"
     gene            1224..>2279
                     /locus_tag="VLS86_10900"
     CDS             1224..>2279
                     /locus_tag="VLS86_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSO51045.1"
                     /translation="MLAAACTDSGGDEGAELPIEIGEGEGSVVIVSWAGYIERGATDP
                     AFDWVTQFEEETGCLVENKVAGTSDEMVALMTGSTEYDLVTASGDSSLRLIAGGSVQE
                     INIDLIESWDTIDPRLQDAAWHTVDGKHYGTPYSSGFNVLGYNTEVFGDTPPDSWNVV
                     FEEQTLPDGESNAGRIQAYDGPIYIADAALYLMATQPELGIEDPYQLTPGEDGTYEVA
                     LDLLRQQRELIGRYWHDAFIQIDDFTNEGVAASSTWPFQTNVLQAGGAPIDKVVPVEG
                     ATGWADTTMMSSNAPHPNCAYMWMEWSLNPKLQGDLAAWFGTVPAVPAACEGNELLGP
                     DGCANNGSESFDEIQFWK"
BASE COUNT          453 a          714 c          730 g          382 t
ORIGIN      
        1 ggacctcgga acctactccc acgcaggccg gctcgagcct cgatagtctc cgccccgatt
       61 tgggaggtca ggatctagtt ggctgcggcc gtgagtctcg acaacgtgag caagctgttc
      121 ggcgaggtga ccgccgttga cggcgtgagc ctcgacatcg ccgacggcga gttcttctcg
      181 atgctgggcc cgtcgggaag cggcaagacc acgtgtctcc ggatgatcgc cggtttcgaa
      241 gtccccagtg acggccgggt cctactgcac ggaagcgacg tgtccgacct ccccccctac
      301 gaccgtgacg tcaacaccgt gttccaggac tacgccctgt tccctcacat gaccgtggaa
      361 caaaacgtcg gctacgggcc gatggtgcgc aaagtcgacg acgccgagcg tgcccagcgg
      421 gtgagtggag cattggcgat ggtccatctg accgagttcg cccaacgcaa gccgagccag
      481 ttgtccggag gccagagaca gcgggtcgcc ttggcgcgag ccctgatcaa ccggccctcg
      541 gtcctcctcc tcgacgagcc ccttggcgcc ctcgatctca agctgcgtca ggcgatgcaa
      601 ctcgagctga agaccctgca acgagaggtc gggatcacct tcatctatgt cactcacgac
      661 caggaggaag cgctgacgat gagcgaccgt ttggcggtgt tcaatcacgg caaggtggaa
      721 caggtgggga cacccaccga ggtgtacgag aaccctcgca acgccttcgt tgccgggttc
      781 gtcggcacct ccaatgtcat ctccggcccg ctcgcccagc gcatcgtcgg atcgagcaaa
      841 ccgttcacgg tgcgacccga gaagatcact atccaggaac cggccagccc cgtcattgac
      901 gggagaacag cggtcgacgg gaccgtggcc gaggtcgtct acctgggcat gtacaccagg
      961 tacagggtcg acatcggtgg cgcggtgatc gaggtcgcgg cgcagaacac ggcggccgaa
     1021 cacgacgatg ctcaaagtca ggttggtcgc caggtcaggc tgacctggat cgaggattcg
     1081 tgccgccctc tcgaggaaag ccctcgagag gtcggcgcga acgaaaaggc cccggccctg
     1141 gccggagccg ggaacaacgt cccagaggag gagattctcg aatgagatca cgaaagtgga
     1201 tattcctgat aacggtgatc gccatgctcg ccgctgcctg taccgattca ggtggcgacg
     1261 agggtgccga gctgccgatc gagatcggcg aaggcgaggg gagcgtcgtc atcgtctctt
     1321 gggccggcta tatcgagcgg ggcgcgaccg atcccgcctt tgactgggtc acccagttcg
     1381 aagaagagac cggttgtttg gtcgagaaca aggtcgcggg cacctcggac gagatggtgg
     1441 cgctgatgac agggtcaacc gagtacgacc tggtcaccgc ctccggggac tccagcctcc
     1501 ggctgatcgc cggtggcagt gtgcaagaga tcaacatcga cctgatcgag agttgggaca
     1561 ccatcgaccc ccgccttcag gacgccgcat ggcacacggt ggacggcaag cactacggga
     1621 cgccgtattc gtcgggattc aacgttctcg gctacaacac cgaggtgttc ggggacaccc
     1681 cacccgactc ctggaacgtc gtcttcgaag agcagaccct ccccgatggc gagtcgaatg
     1741 cgggtcggat ccaggcctat gacggcccga tctacatcgc ggatgccgcc ctgtatctca
     1801 tggccaccca acccgaattg ggcatcgagg atccgtacca gctgacaccg ggagaggacg
     1861 gcacgtacga agtcgccctc gatttgcttc gtcagcagcg ggagctgatc ggccggtatt
     1921 ggcacgacgc cttcatccag atcgacgact tcaccaacga gggtgtcgcc gcctcatcga
     1981 cctggccctt ccagaccaac gtcctgcagg cgggtggagc cccgatcgac aaggtggtgc
     2041 cggtcgaagg tgcaaccggg tgggcggaca ccacgatgat gagctcgaat gctccgcacc
     2101 ccaactgcgc ctacatgtgg atggagtggt cgttgaaccc gaagctccaa ggtgacctgg
     2161 cggcatggtt cggaaccgtg cccgcggtcc ccgcggcctg cgaggggaac gaattgctcg
     2221 ggccggatgg ctgcgccaat aacggcagcg agtccttcga cgaaatccaa ttctggaag
//
LOCUS       DATITW010000781         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_639216, whole genome shotgun sequence.
ACCESSION   DATITW010000781 DATITW010000000
VERSION     DATITW010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639216"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VLS86_10905"
     CDS             complement(<1..390)
                     /locus_tag="VLS86_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSO51046.1"
                     /translation="MEEREIMEIDPPSSVLRRSARPRTATDYVSDSLRRSILNGDLVG
                     GTRLSLAELASTFDVSTTPVREALRELSFEGLVKMDSYRGGTVNAVTRSDVEEIIRIR
                     QVLEPMAVAEAVKGMTPQVLAGAEAILE"
     gene            complement(401..1264)
                     /locus_tag="VLS86_10910"
     CDS             complement(401..1264)
                     /locus_tag="VLS86_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSO51047.1"
                     /translation="MRLANFQVEGERRVGVVVEHSVIDLSNSGLPNDTVDLIRQWDRA
                     KVDHAVAQGARLDVDEVRLLAPLIPRKNIMAVGRNYRDHAQEFSDSGFDASEKQMIPD
                     HPVIFTKSPTSVIGPGDAIVLANDPTGTTDYEGEMAVVIGRTARNVAVESALDHVFGW
                     TIVNDVTARDLQKRHVQWFIGKSPDTFCPMGPWITTIDELPDIGSSWMRTEVNGELRQ
                     EAPISALIFGVETLIATLSEVMTLEPGDVIATGTGKGVGIGFEPPRYLVSGDVVEVSI
                     DGIGTLANPVI"
     gene            1409..2461
                     /locus_tag="VLS86_10915"
     CDS             1409..2461
                     /locus_tag="VLS86_10915"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSO51048.1"
                     /translation="MSGEWWPELGPRWEPTAAVKPPYPTIDIHSHVEVPEAAAVAAPH
                     FRPEYDPRMAFQPEESTRYNRELRATQTAQFVDPAARIADMDLQGIDIQALAIVPPQY
                     FYWLDADTGPKTSAMQNDAIRAMVETNPDRFVGIANLPMDHPEAAVVEMARANSDLGF
                     NGFEVNADVNGGDLDQARFDPVWAKAEELEMLVILHPHGWTEPRRMDDYYLINVVCMP
                     LASTVAVSRMILGGVFERFPGLRVLVVHGGGYLPFYFARTDHAFWVRPETRRHIPRPP
                     SEYLHRLYFDTTVFDPAAVEYLVGEFGADHVLMGTDYPFDMGPTDPLAFLADARLTQE
                     EHALIVGGNAARLLRI"
BASE COUNT          450 a          901 c          793 g          451 t
ORIGIN      
        1 ctccaggatc gcctccgccc cggcgagaac ctggggcgtc atgcctttca ccgcttcggc
       61 aacggccatc ggctccagca cctggcggat gcggatgatc tcctcgacat ccgacctggt
      121 cacggcattg acggtgccgc cgcggtagct gtccatcttg accagaccct cgaacgacag
      181 ctcgcgaagc gcctcgcgca ccggcgtggt gctgacatcg aaagtggagg ccagctcggc
      241 caggctcagc ctggtccccc cgaccaagtc cccgttcaag atcgatcgtc gcagcgaatc
      301 cgacacgtag tcggtggcgg tgcggggtcg cgccgaccga cgcagcaccg agctgggcgg
      361 gtcgatctcc atgatttctc tctcctccac tttcgtcctc tcagatcacg gggttggcca
      421 gggtgccaat cccatcgatg gaaacctcaa ccacgtcccc ggacaccagg taccggggcg
      481 gctcgaaccc gattccgacg cccttgccgg tgccggtggc gatcacatcc ccgggttcca
      541 gggtcatcac ctcgctcagg gttgcgatca gggtctcgac cccgaagatc agggcgctga
      601 tcggagcctc ctgacgcaac tccccgttga cctcggtccg catccaggat gacccgatat
      661 caggaagctc gtcgatggtg gtgatccatg gccccatcgg gcaaaacgtg tccgggctct
      721 tcccgatgaa ccactggacg tgccgctttt gcaggtcgcg tgcggtcacg tcgttgacga
      781 tggtccaccc gaacacgtgg tcgagcgcag actcgaccgc gacattgcgg gccgtgcgcc
      841 cgatgaccac cgccatctcc ccctcgtagt cggtggtccc ggtggggtcg ttggcgagca
      901 cgatggcgtc cccggggccg atgaccgagg tgggcgactt ggtgaagatg accgggtgat
      961 cggggatcat ctgcttctcc gacgcatcga aaccggaatc ggagaactcc tgggcatggt
     1021 cccgatagtt gcggcccacg gccatgatgt tcttgcgcgg gatcaacggc gccagcagcc
     1081 ggacctcgtc cacgtcgaga cgcgcgccct gagccaccgc atgatccact ttggcccgat
     1141 cccattgccg gatcagatcg acggtgtcgt tgggcaatcc ggagttggaa agatcgatga
     1201 cgctgtgctc gacgacgaca ccgacgcgtc gctccccctc gacttgaaag ttggccaacc
     1261 gcatgaccgg cgatggtaca tcgtgcatga tgcatcttgt atcgggggcg agacctgatt
     1321 catgccgatt tctggccaaa accggaggca atgtgagccg caacagattg gggtcgtcgc
     1381 cccgccgtct gaccgacagc ccgaccccat gagcggggag tggtggccgg agttggggcc
     1441 ccgatgggag ccgacggcag cggtcaaacc gccctacccc acgatcgaca tccacagcca
     1501 cgtggaggtc cccgaggcgg ccgcggtcgc ggccccgcac tttcgaccgg agtacgaccc
     1561 gagaatggcc ttccagcccg aggagagcac ccgctacaac cgtgaattga gagccacaca
     1621 gacggctcag ttcgtcgacc cggccgcccg gatcgccgac atggacctgc agggaatcga
     1681 catccaggcc ctggccatcg tcccgcccca atatttctac tggctcgacg ccgacaccgg
     1741 gcccaagacg agcgcaatgc agaatgacgc gatccgggcc atggtcgaga ccaatcccga
     1801 tcgattcgtg ggaatcgcca accttcccat ggaccacccc gaagcggcgg tggtcgagat
     1861 ggcgcgggcc aactccgacc tgggcttcaa cgggttcgag gtcaatgccg atgtgaatgg
     1921 cggcgatctg gaccaggcgc ggttcgaccc ggtttgggcc aaggcggaag agctggagat
     1981 gctggtcatc ctccatcccc acggctggac cgagccgcgc cggatggacg actactacct
     2041 gatcaacgtg gtgtgcatgc ccctggcatc gaccgtcgcc gtgtcccgca tgatcctggg
     2101 gggagtgttc gagcgatttc ccgggctccg ggtgctcgtg gtgcatggcg ggggctatct
     2161 ccccttctac ttcgcccgga ccgatcacgc cttctgggtg cgccccgaga ccagacgtca
     2221 catcccccgg cctcccagcg agtacctgca caggctctac ttcgatacca ccgtcttcga
     2281 tcccgcggca gtggaatacc tcgtcggcga gttcggcgcc gaccatgtcc tgatgggaac
     2341 cgactacccc ttcgacatgg ggcccaccga tcccctcgcc ttcctcgccg acgcccgact
     2401 cacccaggaa gagcacgccc tgatcgttgg cggcaacgcc gcccgactgc tgcggatctg
     2461 aatctctttc gccggtcatc cggcgtcggt gaggacctcg atcacggcac ttatgtcgct
     2521 ccgcccccaa ccccgctgct tggcctcggc catcaccccg gccgcggctg cggtcaccga
     2581 ccctggtggc cgccg
//
LOCUS       DATITW010000782         1531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_478515, whole genome shotgun sequence.
ACCESSION   DATITW010000782 DATITW010000000
VERSION     DATITW010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478515"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1119)
                     /gene="galE"
                     /locus_tag="VLS86_10920"
     CDS             complement(121..1119)
                     /gene="galE"
                     /locus_tag="VLS86_10920"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HSO51049.1"
                     /translation="MRVLVTGGSGFIGSHTAIALIEAGHEPVLVDNLSNSRRSVVARI
                     GEISGVTPEFVEADVRDHEAMRKVVGQGFDACMHFAALKSVGESVEEPLLYYDNNVGG
                     TIALVETLLEFDVRCFVFSSSATVYGDPVTLPLVESVPSGNAVNPYGWTKIMMEQVLR
                     DLHSANSDWSISLLRYFNPVGAHPSGLIGEDPEGDPSNLMPYVARVAAGLLPKVRVFG
                     GDYPTPDGTGVRDFIHVVDLAEGHVKALDRHAQESGVFTYNLGTGEGHSVLEIIKTYE
                     QESGQQIPYDIVERRPGDVASSYADVSKAATELGWKTTRDLAEMCADSWRWQKHPR"
     gene            complement(1146..>1531)
                     /locus_tag="VLS86_10925"
     CDS             complement(1146..>1531)
                     /locus_tag="VLS86_10925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSO51050.1"
                     /translation="LEVEEHPIDLQVFDGSSEVNADPRRTRQILKNLVSNALRHGGET
                     MSVALAKVGDRIEVKVRDSGEAIPDTDVERIFKAFEMGSRGAHPSSVGLGLAVARRLA
                     RLMEGDLTYRYEDGWSSFVLSLPTA"
BASE COUNT          287 a          488 c          471 g          285 t
ORIGIN      
        1 gcatgtcttc cgagtcgatc agctcctgca gggcacccct ggtctccgga tccgggtccc
       61 cgctgatcca agcccgtgcc gcctcgagga tgtcagtcac gggttggagg ctaccgccgg
      121 tcagcgggga tgcttctgcc accgccagga gtcggcgcac atctcggcga gatcccgcgt
      181 cgtcttccag cccagctcgg tagctgcttt ggatacgtcg gcgtagctgg aggcaacatc
      241 ccccgggcga cgttcgacga tgtcgtaggg aatctgttgg cccgattcct gctcataggt
      301 cttgatgatc tccagaacgc tatgaccctc accggtcccc aggttgtagg tgaacacacc
      361 actctcttgg gcatgccgat cgagcgcttt gacatggccc tcggcgagat cgacgacatg
      421 gatgaagtcc cggaccccgg tgccatccgg cgtcgggtag tcacccccga agacgcgcac
      481 cttgggaagc aggccggcag ccacccgggc cacatagggc atcaagttgc tcgggtctcc
      541 ctccgggtcc tcaccgatca gccctgaggg atgcgccccc accgggttga aatagcgaag
      601 cagggagatc gaccagtcag aattggcact gtgcaggtcg cgtagaacct gctccatcat
      661 gatcttcgtc cacccgtagg ggttgaccgc attgccggaa ggcaccgact caaccagtgg
      721 gagcgtgacg ggatcgccat agaccgtcgc cgaagagctg aagacgaagc atctcacgtc
      781 gaactcgagc agggtctcga cgagggcgat cgtgcccccg acattgttgt cgtagtagag
      841 gagcggctcc tccacggact cgccgacgga cttgagggcg gcaaagtgca tgcaggcgtc
      901 gaatccttgg cccaccacct ttcgcatcgc ctcgtgatcc ctgacgtcgg cctccacgaa
      961 ctcgggggtt accccgctta tctcgccaat ccgggccacc accgaccgac gggagttgga
     1021 gagattgtcg accagaaccg gctcatggcc ggcttcgatg agggcaatgg cggtgtggct
     1081 gccgataaag ccggagccac ctgtgacgag aacacgcatg atgtgaggtt agggggtatc
     1141 ggttgtcagg cagtcggcag cgagagcacg aagctggacc acccgtcctc atagcgatag
     1201 gtgagatcac cctccatcaa tcgtgccagg cggcgcgcga cggcgagacc gagcccgacc
     1261 gagctcgggt gggcgcctct actccccatt tcgaaagctt tgaagatccg ctccacgtcg
     1321 gtgtcaggta tggcttcacc cgagtctctg accttgacct cgatccggtc gccgaccttg
     1381 gccagagcca cgctcatcgt ctctccccca tgacgaaggg cattggacac caagttcttc
     1441 aggatctgcc gggtgcgtct tgggtcggcg ttgacctcgg acgagccgtc gaacacctga
     1501 aggtcgatcg ggtgctcttc gacctcgagg a
//
LOCUS       DATITW010000783         1946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_157934, whole genome shotgun sequence.
ACCESSION   DATITW010000783 DATITW010000000
VERSION     DATITW010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157934"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /locus_tag="VLS86_10930"
     CDS             complement(<1..1517)
                     /locus_tag="VLS86_10930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HSO51051.1"
                     /translation="MTSPELTSPEVGLTANEVRQRIDAGLVNQVADSPSRTTGEIVRA
                     NVVTRFNILLGAYLIVVLAVLREPRDALFGIVLVINAAIGIIQELRAKRTLDRLELLA
                     APRVRLVRSGRVTEHAVDQIVVDDLIDLRSGDQLTVDGLVVSSSGLEIDESLLTGEAD
                     PVLKRPDDTCLSGSFVAAGAGRYRATRVGNDAYAARLAHAAKRFTLVRSELREGIDWI
                     LGVVSWAVIPVGALLVWSSIRGGRTFLDGLGGAVAAGVAMIPQGLVLLASIAFAVGVI
                     RLGRSNVLVQELPAIEGLARVDTVCFDKTGTLTEGRLTVEELILLSERDPEPALGALV
                     RVETHPNATLAAISAAFPDAPDWGVQTAVPFSSARKWSGVTFFGAGTWVIGAPDIIAP
                     GDRRAIDIASTVSATGNRVLMLGASDSFLADGRLPSDVVPVAILVLSDQVRVDAAETI
                     RYFADQGIRVKVISGDHPETVAAVARQAGIEGSGEVVDGRRLPEDSEELAAVMER"
     gene            1599..>1946
                     /locus_tag="VLS86_10935"
     CDS             1599..>1946
                     /locus_tag="VLS86_10935"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSO51052.1"
                     /translation="MQSFLFTDIEASTRLWEEHPDHMAAALARHDEILAEAVGRVGGE
                     VVKTTGDGMIAVFDSVAEALSAATQAQKALVAEDWGSTGPLRARMGVHAGDTESRDGD
                     YFGPAMNRTARIMA"
BASE COUNT          343 a          680 c          616 g          307 t
ORIGIN      
        1 tgccgctcca tgaccgccgc cagctcctcg ctgtcttcgg ggagacgacg gccgtcgacc
       61 acctcgcctg agccctcgat ccctgcctgc ctggcgactg cagccaccgt ctccgggtga
      121 tccccggata tcaccttcac ccttatcccc tgatctgcga agtacctgat cgtctcggcg
      181 gcgtcgaccc gaacctggtc ggagaggacc aggatcgcca ctgggaccac gtccgacggg
      241 agccggccat cggccaggaa cgagtcgctg gcgcccaaca tcagcacccg attccccgtc
      301 gccgacaccg tggacgcgat gtcgatcgcc ctccggtcgc caggggcgat gatgtcaggt
      361 gctccgatca cccaggtgcc tgccccgaag aaggtgaccc cgctccactt gcgcgccgag
      421 gagaagggaa ctgctgtctg cactccccaa tccggggcgt cggggaaggc ggcactgatc
      481 gcagccaggg tcgcattggg atgggtctcg actctgacca gggcgcccag cgccggctct
      541 ggatcacgct cggagaggag gatcagctcc tctacggtca gacgcccttc ggtcaaggtc
      601 cccgtcttgt cgaagcacac cgtgtcgacc cgggccagcc cctcgatcgc cggcagctcc
      661 tggaccagga cgttgctcct gcccaacctg atgacgccaa cggcgaaggc gatggaagcc
      721 aacagcacca gaccctgggg gatcatggcc acgccggcgg ccaccgcccc acccagaccg
      781 tcgagaaagg tcctcccgcc ccggatggag ctccacacca ggagcgctcc caccgggatc
      841 accgcccagc tcaccacccc gaggatccag tcgatcccct cacggagctc ggatcggacc
      901 agggtgaacc gtttcgcggc gtgagccagt cgtgcggcat aggcgtcatt gccgacccgg
      961 gtcgcccggt accgcccggc gccggcggcg acgaaactcc ccgagagaca ggtgtcgtcg
     1021 ggccgtttca ggaccggatc cgcctctccg gtgagcaacg actcgtcgat ctccagcccg
     1081 ctggagctga cgaccaaacc gtcgacagtc agctggtctc cagacctgag atcgatcaga
     1141 tcgtcgacca cgatctggtc gaccgcatgc tcggtgaccc gtcccgacct caccaatctc
     1201 actcgtggcg cggccagcag ctccaaccgg tccagggtgc gtttcgcccg cagctcctgg
     1261 atgatcccga tcgcggcgtt gatcacgagg acaatcccga acagggcgtc tcgtggctcc
     1321 cgcaggacgg cgagcacaac gatgaggtac gcaccgagga ggatgttgaa tcgggtgacg
     1381 acattggcgc ggacgatctc gccggtcgtt ctgctcgggc tgtccgcaac ctggttgacc
     1441 aacccggcat cgatgcgctg acgcacctcg ttcgcggtga gccccacttc gggagaggtc
     1501 aactcggggc ttgtcatccg agactcagct taggttcgac ggcgtcacga gagttggcga
     1561 aggcagccgc tccgacgtct ttctatattc gccatgcgat gcagtccttt ctgttcaccg
     1621 acatcgaggc gagcacacgt ctctgggagg agcatcccga ccacatggca gcggctctgg
     1681 cccggcacga cgagatcctg gccgaagcgg tgggccgggt cgggggcgag gtggtgaaga
     1741 ccacgggtga tgggatgatc gccgtgttcg actccgtcgc cgaagccttg tccgccgcga
     1801 cccaggcaca gaaggcactg gtcgccgaag actggggatc caccgggccg cttcgggcga
     1861 ggatgggtgt ccacgccggg gacaccgaaa gccgtgacgg ggactacttc ggcccggcga
     1921 tgaaccggac ggcccggatc atggcc
//
LOCUS       DATITW010000784         3509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_398529, whole genome shotgun sequence.
ACCESSION   DATITW010000784 DATITW010000000
VERSION     DATITW010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398529"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..907)
                     /gene="truB"
                     /locus_tag="VLS86_10940"
     CDS             complement(29..907)
                     /gene="truB"
                     /locus_tag="VLS86_10940"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HSO51053.1"
                     /translation="MTKGFLVIDKPRGMTSNAVVLRVRRATGEKKVGHAGTLDPMATG
                     VVVVAVGPVTRLIRFIQEQPKEYLATAQFGVVTDTLDSDGAVLAREPMDFSEAELLGV
                     ARRFVGTIHQVPPMVSALKHKGRRLYELAREGEVVEREARPVEIHELEIGAVGSGPYP
                     EVEFRVVCGKGTYVRSLADDLAAALGGGAHLTSLRRTRTGSLDVETHGLAIDRLEEWE
                     TRFLAPSDALGDLPAITVTEETARGVCHGMRFVGGEMVGAPENEAVRVLDESGDLIAV
                     YRREGDQARPEVVLAS"
     gene            complement(904..1875)
                     /locus_tag="VLS86_10945"
     CDS             complement(904..1875)
                     /locus_tag="VLS86_10945"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HSO51054.1"
                     /translation="MVDRSLLDQAAKVIADSDELALACHVGPDGDALGSMIGFGMAAR
                     DAGKKVTASFGSPFSVPSNLAFLPTDLLVPPQSFPVSPRVMVVFDVGSADRLAELGSA
                     AARAEHLVVIDHHVTNDGFGHIALIDPQAAATSVLVADLLQILGWPLTPEIATCLHTA
                     LVTDTGRFQYSNTSPSTLRLAADLVAAGARPEQISQKVYEEAPFGYLKVAGIALSRAT
                     LDMDAGLVSTVITEAELDEAGVDWGDIDNLINTLRLAEEADVAVLAKVHADKRVKLSL
                     RSRGGTDVGAIAFELGGGGHRFASGFTYDGPAEDAIDEVRKRIEAHR"
     gene            complement(1868..2206)
                     /gene="rbfA"
                     /locus_tag="VLS86_10950"
     CDS             complement(1868..2206)
                     /gene="rbfA"
                     /locus_tag="VLS86_10950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HSO51055.1"
                     /translation="MLKVNSTLREVIAEEIERLNDARLEMVSITGVDTSPNLRRAIVY
                     VDVLGEADAPAAIAALRRDSSRIQAAIAGQVRMKFTPTLDFQVDPGVLGGERIEAILR
                     SLDKDAEPDG"
     gene            complement(2224..2517)
                     /locus_tag="VLS86_10955"
     CDS             complement(2224..2517)
                     /locus_tag="VLS86_10955"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HSO51056.1"
                     /translation="MRVAALRVELHLPNPQSLKEKRAVLRPLIEGIKRLGSYSVSEVD
                     HHDLWQRAAIGVAVVAPDGESLAMQISKLRRYLDRQMEADVLDVLISELEEPE"
     gene            complement(2517..>3509)
                     /locus_tag="VLS86_10960"
     CDS             complement(2517..>3509)
                     /locus_tag="VLS86_10960"
                     /inference="COORDINATES: protein motif:HMM:NF023413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HSO51057.1"
                     /translation="ANPDTEASGVVVEAQVELGRGPVASVIVQRGTLNTGDSFVAGAV
                     SGRARALMDDTGKQIKSAGPSSPVQIMGWDDVPAAGDFFKVVDSDREARGIAEERQEE
                     LKAASQAMPSARDRLQGLLDQLRSEEATLNVILKADAQGSLEALRDSIPKIEREGGKI
                     QIVHGAIGGINENDVSLAEVTSSVIVGFNVRPEPKARKAAEQAGVQIRSYGIIYELLD
                     DIELMLVGQLAPEEHEQVLGTAEIRALFKVPRAGTIAGCYVVEGVIQRGAKARLIRDG
                     VVIHDGVISSLKRFKDDVREVASGFECGIGFENYNDLKEGDSIEDYLIREVART"
BASE COUNT          584 a         1227 c         1086 g          612 t
ORIGIN      
        1 cagtcggcga tcgggccgct gaggatcttc acgaggcgag caccacctcg ggcctggcct
       61 gatcgccttc cctccgatac accgcgatca ggtcgcctga ctcgtcgagg acgcggaccg
      121 cctcgttctc cggggccccc accatctcac cgccgacgaa acgcatcccg tgacagacac
      181 cgcgggcggt ctcttcggtc accgttatcg ccggcaggtc accgagcgca tcggatggag
      241 ccagaaaccg cgtctcccat tcctcgagcc ggtcgatcgc cagaccgtgg gtctccacgt
      301 ccaatgaccc ggtgcgggtg cgacgcagtg aggtcaggtg tgccccaccg cccagggcag
      361 cggcgagatc gtcggcaagc gagcgcacat aggtcccttt cccgcacacc accctgaact
      421 ccacctccgg atatggcccg gacccgacag ctccgatctc caactcgtgg atctccaccg
      481 ggcgggcctc ccgctcgacc acctcaccct cacgggcgag ctcgtagaga cgacgaccct
      541 tgtgcttgag ggcggagacc atcggcggca cctgatggat cgtccccacg aatcgccggg
      601 ccacgccgag aagctcggct tcggagaagt ccatgggctc cctggccagg accgctccat
      661 cggagtcgag ggtgtcggtg acgaccccga actgagccgt cgccaagtac tcctttggct
      721 gctcctggat gaatctgatc aacctggtca ccgggcccac ggccaccacc accactccgg
      781 tcgccatcgg gtcgagggtg ccggcatgac cgaccttctt ctcgcccgtg gcgcggcgga
      841 cccggagcac cactgcgttg gaagtcatgc ctctcggctt gtcgatgacc agaaagccct
      901 tggtcatcgg tgggcctcga tcctctttcg cacctcgtcg atggcgtctt ctgccgggcc
      961 gtcataggtg aatcccgagg cgaagcggtg ccccccacct cccagctcga acgcgatggc
     1021 cccgacatcg gtgccacccc gtgagcgcag gctcagcttc actcgtttgt cggcatggac
     1081 cttggccagg acggcgacgt cggcctcctc ggcgagacga agggtgttga tcaggttgtc
     1141 gatgtcgccc caatccactc ccgcctcatc cagctcggct tcggtgatga ccgtcgagac
     1201 cagaccggcg tccatgtcca gcgtggcccg ggacagggct atcccggcca ccttcaaata
     1261 cccgaacggc gcttcctcgt acaccttctg gctgatctgc tccggtcggg cgccggctgc
     1321 gaccaggtcg gcagccagcc tcaaagtcga aggtgaggtg ttcgagtact gaaaccgccc
     1381 ggtgtcggtg acgagggcgg tgtggaggca ggtcgcgatc tccggggtga gcggccaacc
     1441 cagtatctgg aggaggtccg ccaccaggac gctggtggca gcggcctggg ggtcgatcag
     1501 ggcgatgtgg ccgaacccgt cgttggtgac gtggtggtcg atcaccacca ggtgctcggc
     1561 tcgagccgcc gccgacccca actccgccag acggtcggcg gagccgacgt cgaacacgac
     1621 catgacccga ggtgagaccg ggaagctctg gggcggcacc aggaggtcag taggcaggaa
     1681 ggccaggttg gaggggaccg agaacgggga tccaaagctg gcggtgacct tcttgccggc
     1741 atcgcgggcc gccatcccga atccgatcat cgatcccagc gcgtctccat cggggccgac
     1801 gtggcaggcc aatgccaact cgtcgctgtc ggctatcacc ttcgccgcct gatcgaggag
     1861 ggagcggtca accatcaggc tctgcgtcct tgtccaggga gcgaaggatg gcctcgatcc
     1921 tctcgcctcc aagcaccccg ggatcgacct ggaagtcgag tgtcggggtg aacttcatcc
     1981 tcacctggcc ggcgatcgca gcctgaatcc ggctcgagtc ccggcgcaag gcggcgatgg
     2041 cagccggtgc gtcggcttcg cccaggacgt ccacatagac gatggcgcgg cgcagattgg
     2101 ggctggtgtc gacgccggtg atcgagacca tctcgagtct ggcgtcgttg agacgctcga
     2161 tctcctcggc gatcacctcg cgcagcgtgg agttgacctt gagcatccgc gggctcgcct
     2221 ctctcactct ggttcctcca actcgctgat gagcacgtcg agcacatccg cctccatttg
     2281 ccggtcaagg tagcgacgaa gcttggaaat ctgcatggcg aggctctcgc cgtcgggggc
     2341 cacgaccgcc accccgatcg cagcccgttg ccacagatcg tgatgatcca cttcggagac
     2401 gctgtacgac ccgagacgct tgatcccctc gatcagcggt cggaggacgg cccgtttctc
     2461 cttcagcgac tgagggttgg ggagatgcag ctcgacccgc agggccgcga cccgcatcag
     2521 gtgcgggcca cctcgcggat caagtagtcc tcgatgctgt ctccttcctt gaggtcgttg
     2581 tagttctcga agccgatccc acactcgaac ccggatgcca cctcacgcac atcgtccttg
     2641 aaccgcttca gggaggagat gaccccgtcg tggatcacca caccgtcgcg gatcagcctc
     2701 gccttggccc cgcgctggat cacgccctcg accacatagc aaccggcgat ggtccccgcc
     2761 cgtggcacct tgaacagagc ccggatctcg gcggtcccca gcacctgctc gtgctcctcg
     2821 ggggcaagct ggcccacgag catcagctcg atgtcgtcca ggagctcata gatgatcccg
     2881 tagctccgga tctggacgcc ggcttgttcc gcagcctttc gggcctttgg ctccgggcga
     2941 acgttgaacc ccacgatcac cgagctcgtc acctcggcga gactcacatc gttctcgttg
     3001 atgcccccga tcgcaccgtg gacgatctgg atcttcccgc cctcccgctc gatcttcgga
     3061 atcgagtcgc gcagtgcctc gagtgatccc tgggcgtcgg ccttgaggat gacgttgaga
     3121 gtggcctcct cggaacgaag ctggtcgagc agaccctgga gtcgatcgcg ggcagaaggc
     3181 atcgcctggc tggctgcctt caactcctct tgacgctcct cggcgatccc tcgcgcctcg
     3241 cggtcgctgt cgaccacctt gaagaagtca ccggcggcgg gcacgtcgtc ccagcccatg
     3301 atctggaccg gggacgaggg cccggccgac ttgatctgct taccggtgtc atccatcaaa
     3361 gccctggccc gccccgacac cgccccggcc acgaaggagt cgcccgtgtt caaggtcccg
     3421 cgctggacga tgaccgaggc aaccggcccc cttcccagct cgacctgtgc ctcgaccacg
     3481 acgccagagg cctcggtgtc gggattggc
//
LOCUS       DATITW010000785         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_559325, whole genome shotgun sequence.
ACCESSION   DATITW010000785 DATITW010000000
VERSION     DATITW010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559325"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1294
                     /locus_tag="VLS86_10965"
     CDS             161..1294
                     /locus_tag="VLS86_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HSO51058.1"
                     /translation="MIYLDHAATTPMGPGVWDAMRPFSEEVFGNSSGIHETSRRAKNA
                     LEEARERIAATIGARPLEIVLTSGGTESDNLALKGSALSPGPRRGVVTLTTEHEAVLE
                     SADYLRRLQFPTAVVGVDESGAVDVEALLDAIDPETAVVSVMHVNNETGVVQDLAGLV
                     AAVKGRDPGVLFHSDAVQAFASEMIDVDELGVDLLTITGHKFGGPKGAGLLYVREGTP
                     LEPVLHGGGQELGRRSGTHDVAGAVGLATAMEAAAGDRARFRSQVGQIRDDFEKRMLA
                     GVDDLVVNTPDTGRSPQHLNLRFPGVRNETLLVRLDRAQVAASAGSACQSGASEVSHV
                     LEGMGLSADQARESVRFSFGWNSTPDEAIEASDIVLGLIEELR"
     gene            1291..2340
                     /gene="mnmA"
                     /locus_tag="VLS86_10970"
     CDS             1291..2340
                     /gene="mnmA"
                     /locus_tag="VLS86_10970"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HSO51059.1"
                     /translation="MRVMVAMSGGVDSSVAAALMRQEGHEVVGVTLKLWSGPNGEAPT
                     AGCCTVSDAEDARRVAAQLDIPYYVLDYTEEFRDGVVDRFVADYASGRTPNPCIECNR
                     TVKFDRLLRRLAEFDAEALVTGHHARSRFQDGSWQLHRGADQGKDQSYVLSMLTQFEL
                     SRTRFPVGELTKTEVRRLAADAGLRTAAKPESMDICFVGQRDYRGFLRDTAPAAFEPG
                     EVVGSDGAELGRHDGVIGFTIGQRRGLGVAVGEPRFVIRIDPGTSTVVIGSRDELAVA
                     GAKLRQLTWTTGEAVEGRVMAQFRAHGEPVSAALHDGRLLFSTPQSAVAPGQTVAFYD
                     GDRVLGGGLIADTYS"
     gene            2366..>2890
                     /locus_tag="VLS86_10975"
     CDS             2366..>2890
                     /locus_tag="VLS86_10975"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HSO51060.1"
                     /translation="MSSEATRIDELRSQIEQLRSQVNEHLYRYHVLDDPTISDAQFDA
                     LIGELRRLEEESPELVTPDSPTQRVGAPVGDLFKPVRHLRPMFSLDNADTADVLLAWE
                     QRMERQLGRPPSSYVAELKIDGLAVVLTYRDGVLATGATRGDGVTGEDITANLRTIKS
                     VPLRLLGDSPPAIIE"
BASE COUNT          478 a          944 c          988 g          480 t
ORIGIN      
        1 cctctcgcct ccagctcggg gatcaagcgc tgccaacacc aggacccgtg ataggcccca
       61 tgaaccagga cgaaatccaa ctcaacctcc ggtgacctcc gatgctaggt cgtggtgcag
      121 ttgtggccca gccccgggat cctgcgacaa tctgcagcga atgatctatc tcgaccacgc
      181 cgccaccacg ccgatgggtc ccggtgtctg ggacgcgatg cgtcccttct ccgaggaggt
      241 gttcgggaat tcctcgggca tccacgagac ctcgcgacgc gccaagaacg ccctggagga
      301 ggcgcgggag aggatcgccg ccaccatcgg ggcccgacct ctggagatcg tgctcacctc
      361 gggcgggacc gagtcggaca acctcgcgtt gaagggatcg gcactcagcc cgggcccgcg
      421 gcgcggggtg gtcacgctga cgaccgagca cgaggcggtg cttgagagcg ctgactacct
      481 tcgtcgtctc cagttcccga cggctgtggt cggcgtcgac gagtcgggcg cggtcgacgt
      541 ggaagcactt ctcgacgcga tcgatcccga gacggccgtg gtctcggtca tgcacgtcaa
      601 caacgagacc ggtgtcgtgc aggacctggc aggtctggtc gccgcggtca aggggcgcga
      661 tcctggcgtc ttgttccatt ccgacgcggt ccaggcattc gcctcggaga tgatcgatgt
      721 cgatgagctg ggtgtcgacc tgctcacgat caccgggcac aagttcggcg ggcccaaagg
      781 cgccggcctt ctctatgtcc gggagggaac ccccctggag ccggtgctcc atggtggtgg
      841 ccaggagctg gggcgacgct ccggcaccca cgacgtagcg ggggcggtgg gtctggccac
      901 ggcgatggag gcggcggccg gcgaccgggc gcgattccga agccaggtgg gccagatccg
      961 tgacgacttc gagaagcgga tgctggcagg ggtggacgac ctggtggtga acacccccga
     1021 caccggtcgc tccccacagc atctgaacct gaggttcccc ggggtccgca acgagacgct
     1081 cttggtccgc ctcgaccggg cccaggtggc cgcttcagcc ggctcggcgt gccagtcggg
     1141 tgcgtccgag gtgtcccacg tcctcgaggg aatgggactg agcgccgacc aggcgcgaga
     1201 gagcgtgcga ttcagctttg ggtggaacag cacccctgac gaagcgatcg aagcctccga
     1261 catcgtcctc gggttgatcg aggagctccg atgagagtga tggtcgccat gtccggtggc
     1321 gtcgactcgt cggtcgcggc cgccttgatg agacaggaag gccacgaggt ggtcggtgtc
     1381 accctcaagc tgtggtccgg ccccaacggg gaggctccca ccgccgggtg ctgcacggtc
     1441 tccgacgccg aggacgcgcg acgggttgcg gctcagctcg acatccccta ctacgtgctg
     1501 gactacaccg aggagttccg ggacggggtg gtcgatcgct ttgttgccga ctatgcctcg
     1561 ggccgaaccc cgaacccatg catcgagtgc aaccggaccg tgaagttcga tcgtcttctc
     1621 cgtcgtctgg ccgaattcga cgcagaggca ctggtcaccg gtcatcatgc ccgcagtcgt
     1681 ttccaggacg gctcgtggca gctgcatcgg ggggcggatc agggcaaaga ccagtcatac
     1741 gtcctgtcca tgctcaccca gttcgagctg tcgaggacca ggttcccggt cggggagttg
     1801 accaagaccg aggtgaggcg tctggctgca gatgccgggt tgcgcaccgc ggccaagccg
     1861 gagtcgatgg acatctgctt cgtgggtcag cgtgactatc ggggttttct ccgcgacacc
     1921 gctccggccg cattcgagcc gggagaggtg gtcgggagcg acggcgccga acttgggcgc
     1981 cacgacggag tgatcgggtt caccatcggc cagcgtcgcg gcctgggagt ggcggtgggc
     2041 gagccgcgat tcgtgatccg gatcgacccc ggcacctcca ccgtggtgat cgggtcccgt
     2101 gacgaactgg cggtggcggg ggccaagctc cgtcaactga cttggaccac gggggaggcg
     2161 gtcgagggcc gggtgatggc ccagttccgg gcccacggcg aaccggtttc cgcggcgctt
     2221 cacgacggga ggcttctctt ctccacgccg cagagcgccg tggctccggg tcagaccgtc
     2281 gccttctatg acggcgatcg tgtgctcggc ggcgggctca tcgccgacac ctattcctga
     2341 tgcgcgcccg gtaacttcga tctcgatgtc gtctgaagca acgcgaatcg acgagcttcg
     2401 ctcacagatc gagcagctgc gctcccaggt caacgagcat ctctatcgct accacgtcct
     2461 cgatgacccg acgatctcag acgcacaatt cgacgccctg atcggcgagc tgcgccgact
     2521 cgaggaggag agcccggagc tggtcacccc ggacagcccg acgcagcggg tcggggcacc
     2581 ggtcggggac ctgttcaagc cggtccgtca tctccggccc atgttctccc tggacaacgc
     2641 cgacacggcg gacgtgctgc tcgcctggga gcagcgcatg gagcgccagt tgggccgccc
     2701 accctccagc tatgtcgccg agctgaagat cgacgggctc gctgtcgtcc tcacttatcg
     2761 cgacggagtc ctcgctaccg gggcgacacg gggcgacggg gtcaccggcg aggacatcac
     2821 ggccaacctg cgcacgatca agtcagtccc gctgagactt ctcggagaca gcccacccgc
     2881 catcatcgag
//
LOCUS       DATITW010000786         2304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_358481, whole genome shotgun sequence.
ACCESSION   DATITW010000786 DATITW010000000
VERSION     DATITW010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358481"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VLS86_10980"
     CDS             <1..701
                     /locus_tag="VLS86_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HSO51061.1"
                     /translation="RFGFGVYWAQIVVPRDSDIETIEDLEALTWAIPDLGSTSGYLVP
                     LVMLDEAGVTPGETVETGGHPNAALAVYRGDAEFGTTFYSPPLIADGQWEIGDPPEIP
                     DEYIDSCAVDAEGDLYCGPNQEYRVLDARASATTDAPDIVQQVRILAISPEIPNDTLS
                     FGPDFPAEQRTQIEEALVACAAECEDDENCAWNDSIGNQDFYGWTGIEPATDAEYDAL
                     RAVVETAGIELEDL"
     gene            759..1511
                     /gene="phnC"
                     /locus_tag="VLS86_10985"
     CDS             759..1511
                     /gene="phnC"
                     /locus_tag="VLS86_10985"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HSO51062.1"
                     /translation="MLQVEHLTKVYDDGTVALKDVSFEVPDGQFLAIIGLSGSGKSTL
                     LRCINRLVDPTEGRIIWNGEDITAASQEDLRKIRRRIGMVFQHFNLVHRSKVITNVLA
                     GRLGYTSPALSVANRFSKIDRERAQAQLARFGLEGKEQNRSEELSGGQQQRVGIARAM
                     MQEPEMILADEPVASLDPVLAHSIMQHLEDINKTDGVTVLCSLHFLDLVQRYGQRVIA
                     LNEGQLVFEGLPSEIDDAMFKDIYGQEAERVG"
     gene            1504..>2304
                     /locus_tag="VLS86_10990"
     CDS             1504..>2304
                     /locus_tag="VLS86_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51063.1"
                     /translation="MADLIHEEKAVSPETPDEELKTSPRPRRRRTLWLVLGMLAAFVI
                     YAYGFKVTEVNLDRINDETRQESLFRVIRALGQPDLLTYDVAETSVDLEYAIPCPAGG
                     FQPEPMEGSATFTVEPVCADPRSVVIVRGEGFRANDEVQVFFVPPNGTELRLENVRTD
                     ADGGFETTARLPARPDTEVQTVRVKSKLNVGSVFSPVQVEQPDGTMARSPRWSQNAID
                     TLDKIIETVFLALLATTIGTVVAVPLSFFAARNLMRDIRLPPLQLGLAI"
BASE COUNT          443 a          752 c          729 g          380 t
ORIGIN      
        1 ttcgcttcgg gttcggcgtc tactgggccc agatcgtggt gcctcgggac agcgacatcg
       61 agaccatcga ggacctcgag gccctgacct gggcgatccc cgacctggga tcgacctcgg
      121 gctatctggt gccactggtg atgctcgacg aggcaggggt cacccccggt gagaccgtcg
      181 agaccggtgg acatcccaac gcagccctgg ccgtttaccg gggtgacgcc gagttcggga
      241 cgaccttcta cagcccacca ctgatcgccg acgggcaatg ggagatcggc gatccgccgg
      301 agatcccgga cgagtacatc gactcttgcg ccgtggacgc agagggagac ctctactgcg
      361 ggcccaacca ggagtaccgg gtcctcgacg caagggccag cgccaccacc gacgcccccg
      421 acatcgtcca gcaggtgagg atcctcgcca tctcgcccga gatccccaac gacaccttgt
      481 cgttcgggcc cgacttcccc gccgagcagc ggacacagat cgaggaagcc ctggtcgcct
      541 gtgccgcgga gtgcgaggac gacgagaact gcgcctggaa cgactccatc ggcaaccagg
      601 acttctacgg ctggaccggg atcgagccgg caaccgacgc cgaatacgac gctctgcgcg
      661 cggtggtgga gaccgccggg atcgaactgg aagatctgta gacgagcaat gaggagggag
      721 acgggaaccg tcgtctccct cctcatctcc ggaggagcat gctccaggta gagcacctga
      781 ccaaggtgta cgacgacggc acggttgccc tcaaagacgt gtcattcgag gtgcccgacg
      841 gccagttcct ggcgatcatc ggcctctccg gatcgggcaa gtcgacactg ctccgctgca
      901 tcaaccgtct ggtcgatccc acggagggtc gaatcatctg gaacggtgag gacatcaccg
      961 cggcctcaca ggaggacctg cgcaagatcc gacggcgaat cggcatggtc ttccagcatt
     1021 tcaatctggt ccatcgctcc aaggtcatca ccaacgtcct cgccgggcga ttgggctaca
     1081 ccagcccggc gctgtccgtc gccaatcggt tctcgaagat cgaccgcgaa cgagcgcagg
     1141 cccaactggc tcgattcgga ctcgaaggca aggagcagaa ccggtccgag gagctgagcg
     1201 gtggccagca gcaacgggtc ggcatcgcca gggcgatgat gcaggagccc gagatgatcc
     1261 tcgccgacga gccggtggcc agtctcgacc cggtcctggc ccattcgatc atgcagcacc
     1321 tggaggacat caacaagacc gacggggtca ccgtgctctg cagcctgcat ttcctcgacc
     1381 tggtccagcg ctatgggcaa cgggtgatag ccctcaatga ggggcagctg gtgttcgaag
     1441 ggctcccttc ggagatcgac gacgcgatgt tcaaggacat ctacggccag gaggccgagc
     1501 gggttggctg acctcatcca cgaggagaag gcggtttcgc cggagactcc ggacgaggag
     1561 ctcaagactt caccccgacc acgacggcga cgaaccctgt ggttggtcct cgggatgctc
     1621 gccgccttcg tcatctacgc gtacgggttc aaggtcaccg aggtgaatct ggatcggatc
     1681 aacgacgaga cccgccagga gtccttgttc cgggtgatcc gggccctggg ccaacccgac
     1741 ctgctgacct acgacgtcgc ggagacctcg gtcgacctcg aatacgcgat cccatgcccc
     1801 gccgggggtt tccaacccga gccgatggag ggcagcgcca ccttcaccgt cgagccggtc
     1861 tgcgccgacc ctcgatccgt ggtgattgtg cggggcgagg gtttcagggc caacgacgag
     1921 gtacaggtgt tcttcgtgcc tccgaacggg accgagcttc gactcgagaa cgtgcgcacg
     1981 gatgccgacg gtggcttcga gacgaccgcg aggcttccgg cacgtcccga caccgaggtg
     2041 caaacggttc gggtgaagag caagttgaat gtgggaagcg tcttcagccc ggttcaggtc
     2101 gaacagcccg atgggaccat ggcacggtcg ccacgatggt cccagaacgc gatcgacacc
     2161 ttggacaaga tcatcgagac cgtgtttctc gcccttctcg ccaccacgat cgggaccgtg
     2221 gtcgcggttc ctctctcctt cttcgccgcc cgcaacctga tgcgtgacat ccgcctgcca
     2281 cccctgcagc tgggtcttgc cata
//
LOCUS       DATITW010000787         1596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_639516, whole genome shotgun sequence.
ACCESSION   DATITW010000787 DATITW010000000
VERSION     DATITW010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639516"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VLS86_10995"
     CDS             complement(<1..476)
                     /locus_tag="VLS86_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSO51064.1"
                     /translation="MMGTSTVAPFYVESAVAGGVKPGVAGGLFAVGSVSAVVGRLIWG
                     HIASLRTGVHFAMIAGFQALGAASFVLLAFFGSVLPLAAATILLFAAGWGWPGLLTFA
                     IVSRSPQAPALAAGIVASGQFGGGIFGPLGFGLLVEATSYRVAWLAGAALLVIGATL"
     gene            complement(597..1097)
                     /locus_tag="VLS86_11000"
     CDS             complement(597..1097)
                     /locus_tag="VLS86_11000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSO51065.1"
                     /translation="MPSVLFTATLSATLGTLPMFLVGALAIFIRADLGFGESALGLAA
                     SLFYLFAALGSSPGGRLAERVGERPAMAVAAALTFASSVGLAWLTDGWLALAICLVLA
                     GIAVGIAQPASSLALVRGIPFHRQGMAFGLKQSSGAFGTLLGGMSVPLIGLTVGWRWA
                     FLGAAI"
     gene            complement(1115..>1596)
                     /locus_tag="VLS86_11005"
     CDS             complement(1115..>1596)
                     /locus_tag="VLS86_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSO51066.1"
                     /translation="PRAVRDEKFWRNLCDAVSRPDLKNDPRTIDNGARVANRRFLVEL
                     LDEVFITRTKDEWMAILDEFDIPAAPVNDFAGIFSDPQVEARQMVKTYEHPTLGAIRY
                     SPSPMKFSDWEFPNRPAPMLGEHTREVLSDRLGFSSGDIDRLTAGGVVKTWPETDPG"
BASE COUNT          334 a          536 c          525 g          201 t
ORIGIN      
        1 agcgtggcgc cgatgactag gagagccgcc ccggcgagcc aggcgacccg gtatgaggtt
       61 gcttcgacca gaagtccgaa tcccagaggt ccaaaaatac cgcccccgaa ttggccggac
      121 gcgacaatcc cggcggcgag ggcaggcgct tgcggactgc gggagacgat ggcgaaggtc
      181 aggaggcccg gccaacccca cccggccgcg aagagaagaa tggtggcggc ggccagcggc
      241 aacaccgacc cgaaaaatgc cagcagcacg aacgaggcgg cgccaagtgc ctggaatccg
      301 gcgatcatgg cgaagtgcac gccggtccga agactggcga tatgtcccca gatcaggcgc
      361 ccgaccaccg ccgagacgct gcccaccgca aagagtcctc ccgccactcc cggcttgacg
      421 ccacccgcca ccgccgactc gacgtagaag ggcgccacgg tgctcgttcc catcaccgcg
      481 aacaggctcg cgatcgatag gtaccggagg ggccggatgg ccaggtcgtt cctcgttcca
      541 cgatcgagcg cctccggctg cctggacccg cgatgaagcc tgataagggg aaggcactag
      601 atcgccgcac cgaggaacgc ccagcgccag cccacggtca atccgatgag agggaccgac
      661 atgcctccca gcaacgtccc gaaagcgccc gacgactgct tcaaaccaaa ggccatcccc
      721 tgccgatgga atggaatccc ccgcaccagg gccaagctcg atgccggttg ggcgatcccg
      781 acggcgatcc ccgccagcac caggcagatg gccagcgcca gccaaccgtc ggtcaaccag
      841 gcgaggccga cgctggaggc gaacgtgagg gcggccgcca ctgccatcgc tgggcgctcg
      901 cccactcgct cggcgagacg accgccgggc gaactcccca gggccgcaaa gaggtagaag
      961 agggacgcag ccaatccgag ggcggactca ccgaagccga ggtcggctct gatgaagatg
     1021 gccagcgcac cgaccaggaa catcggcagc gtcccgaggg tggccgacag ggtggccgtg
     1081 aagaggacgg agggcaccga ggggccgacg acgttcagcc gggatcggtc tccggccacg
     1141 tcttcaccac ccccccggcc gtaagccgat cgatgtcccc ggaagagaag ccgaggcgat
     1201 ccgaaagcac ctcccgggtg tgctccccga gcatgggggc aggacgattg gggaactccc
     1261 aatcgctgaa cttcatggga ctcggtgaat agcggatcgc gcccagggtc ggatgctcgt
     1321 aggtcttcac catttgacgg gcctcgacct gggggtccga gaagatgccc gcgaagtcat
     1381 tgacgggggc ggcggggatg tcgaactcgt cgaggatggc catccattca tccttcgtcc
     1441 gggtgatgaa gacttcatcc aacaactcga cgaggaaccg acggttggcg acccgggcgc
     1501 cgttgtcgat ggtgcgggga tcattcttca agtcgggtcg gctcaccgca tcgcacagat
     1561 tccgccagaa cttctcatca cggaccgccc gaggcc
//
LOCUS       DATITW010000788         1562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_278698, whole genome shotgun sequence.
ACCESSION   DATITW010000788 DATITW010000000
VERSION     DATITW010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278698"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VLS86_11010"
     CDS             <1..585
                     /locus_tag="VLS86_11010"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSO51067.1"
                     /translation="IKGFQATFDAGGNERSAWTLQEAINTLEGLEKPTIAAVHGYALG
                     GGLELAMGADFRYLAEDAQAGQPEILLGLIPGAGGTQRLARIVGYQRAKELILTGRQV
                     SADEALAIGLADKVLPAEDLLEAALRDAREWATRPTRAVAAAKRALNAGFGLPVSEGL
                     LIERDAFNETFATEDAREGVAAFIDKRPAEFKGR"
     gene            complement(572..1009)
                     /locus_tag="VLS86_11015"
     CDS             complement(572..1009)
                     /locus_tag="VLS86_11015"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSO51068.1"
                     /translation="MIQITMDFAAPPQRLWEELADLASHSEWMADAEDVRFVTDQRSG
                     VGTQMRVPTRIGPLRTNDLMTVVEWEEGRVIAVEHVGAVSGVGRFEVVATGEGARLLW
                     SESLRFPWWLGGRIGAWVAGPILARVWRGNLARLGERVTLSDP"
     gene            complement(1006..>1562)
                     /locus_tag="VLS86_11020"
     CDS             complement(1006..>1562)
                     /locus_tag="VLS86_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO51069.1"
                     /translation="CEVVDSPDATVVGHSMLGLAIPLIPERVAVKRLVFLCAFLPEPG
                     SSFKELREREDIEPDREMQHAQFTNLGEDVWMVGPETANELFYHDAPSEIAAWSVPLL
                     RPQAYRITREKTPLTAWPDVPCSYVLCRDDRVIDSTWARRAARERLGVEALELDGGHS
                     PFLTRPGELATVLDGLETDSRNQS"
BASE COUNT          274 a          539 c          497 g          252 t
ORIGIN      
        1 atcaagggct ttcaggccac cttcgacgcc ggtggcaacg agcgctcggc ctggaccctc
       61 caggaggcga tcaacaccct ggagggcttg gagaagccga ccatcgctgc ggttcacggc
      121 tacgccctcg gtggcgggct cgagttggcg atgggcgccg acttccggta tctggccgaa
      181 gacgcccagg ccggccagcc cgagatcctg ctcgggctca tccctggagc cgggggtacc
      241 cagcgtctgg cccggatcgt gggctatcag cgagccaagg agttgatcct gaccgggcga
      301 caggtcagcg ccgatgaggc cctggccatc gggctcgccg acaaggtgct gccggcagag
      361 gatctgctcg aggccgcact gcgcgatgcc agggaatggg ccacgagacc gacccgcgcc
      421 gtggccgctg cgaagagagc cttgaacgcc gggttcgggc ttccggtgag cgaagggctg
      481 ctcatcgagc gcgacgcgtt caacgagacc ttcgccaccg aggacgcccg ggagggtgtt
      541 gccgccttca tcgacaagcg ccccgccgag ttcaagggtc gctgagcgtc acccgctctc
      601 cgagtcgggc gaggttcccc ctccacaccc gcgccaggat cggccccgcc acccacgctc
      661 cgatccgtcc cccaagccac cacgggaacc ggagcgattc gctccagagc agccgggcgc
      721 cctccccggt cgccaccacc tcgaagcggc cgactccgga gaccgcgccc acatgctcca
      781 ccgcgatcac acgcccttct tcccactcga ccaccgtcat caggtcgttg gttcgcagcg
      841 ggccgatgcg ggtgggcacc cgcatctggg tccccacccc gctcctctga tcggtcacga
      901 agcgaacgtc ttcggcgtcg gccatccatt ccgagtgaga ggcgagatcg gccagctcct
      961 cccacaaacg ctgcggtggc gcagcgaaat ccatggtgat ctggatcaac tttgattcct
     1021 cgagtcggtt tcgaggccgt caagcactgt tgccagctcg ccgggccggg tgagaaaggg
     1081 cgagtgcccg ccgtcgagct cgagcgcctc cacgccgagt cgttctcttg ccgcccgcct
     1141 tgcccaggtc gagtcgatga cacggtcgtc gcggcacaga acatatgagc aggggacatc
     1201 gggccaagcc gtcagggggg tcttctccct tgtgattcga taggcctggg gccgaaggag
     1261 cggcacggac caagcggcga tttccgacgg cgcatcgtgg tagaagagct cgttggccgt
     1321 ctccggcccc accatccaaa cgtcttcgcc gagattcgtg aactgggcgt gttgcatttc
     1381 tcgatccggc tcgatgtcct cccgctcccg caactctttg aagctggagc caggttcggg
     1441 aagaaaggcg caaaggaaca cgagacgctt cactgcaaca cgttccggga tgagtgggat
     1501 agccagaccc aacatggagt gtccgaccac tgtcgcatcc gggctgtcga caacctcaca
     1561 ga
//
LOCUS       DATITW010000789         1822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_719906, whole genome shotgun sequence.
ACCESSION   DATITW010000789 DATITW010000000
VERSION     DATITW010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719906"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1448)
                     /locus_tag="VLS86_11025"
     CDS             complement(198..1448)
                     /locus_tag="VLS86_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSO51070.1"
                     /translation="MTEDRSGDVTHPESEMMRRGWDPADGLNSLKPPLFQTSTFVFES
                     SAAAERLFEVVYGGAAPEEGEEIDFIYTRMDHPNLVIVEGRLAVWDNAESALVFSSGV
                     SAIFTTLLTFARPGDLILHSAPLYGGTNTMLHKVLGPLGYLAAGYGPGATESEIEALI
                     DGRRLGAIYVETPANPTNDVFDIAMASRVAAEHSDTGHRVLTIVDNTFLGPYAQRPLD
                     HGADLVVYSATKYFGGHSDLVAGVVTGPTDLIEPLKTMRYRIGTTADPHTAWLLGRSM
                     ETYSIRVEKQTANAASVAEFLAGHPKVASVRYLGLIGDDDPNADLAKRQWSSGGAMIS
                     FDVVGGKQEAFRFLDAMRLIANTTSLGGTESIACHPWTTTHSNVDQETKKQIGVTEAM
                     IRLSIGIENADDIIGDLEHALARV"
     gene            1500..>1822
                     /locus_tag="VLS86_11030"
     CDS             1500..>1822
                     /locus_tag="VLS86_11030"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HSO51071.1"
                     /translation="METNLAVDVAYAVEILRPTRRILVFTGAGISTESGIPDFRGPEG
                     LWTKVDPEDFHIDRYRANPELRKRGWRMHLDGELWGARSTVRPNLGHEAIKRLADSGR
                     LSGVVT"
BASE COUNT          327 a          570 c          602 g          323 t
ORIGIN      
        1 gaaatcgcgt ggaaagactt cgaaccgcgg tggggcaaag atatcgggcg cggctgggag
       61 gcggctctgt aaatccccca aaaaacaatc agcccgctgc tcattgtcct ctccctcagt
      121 cacgcctccc aagcgcgtcc cgccgccatt attccaaaac ccgccccagg gcagaatggg
      181 cttttcgacc tatgtcttca cactcgcgct agcgcgtgct cgaggtcgcc aatgatgtcg
      241 tcggcgttct cgatcccgat cgagagccgg atcatcgcct cggtgacccc gatctgcttc
      301 ttggtctcct ggtccacgtt ggaatgggtg gtggtccagg ggtgacaggc gatcgactcg
      361 gtgccaccga gagaggtggt gttggcgatc agccgcatcg cgtcgaggaa gcggaacgct
      421 tcctgctttc cgccgacgac gtcgaaggag atcattgcgc cccccgacga ccactgccgc
      481 ttggcgaggt cggcattggg atcgtcgtca ccaatcagcc ccaggtagcg aacactcgcc
      541 accttgggat gacccgcgag gaattcggcg accgaggccg cgttggccgt ctgcttctcg
      601 accctgatcg agtaggtctc catgctcctg ccgagtagcc atgcggtgtg cgggtcggcg
      661 gtggtgccga tccggtaccg catcgtcttc agtggttcga tgaggtcggt gggcccggtg
      721 acaacaccgg ccacgaggtc gctgtgcccg ccgaagtact tcgtcgccga gtagaccacc
      781 agatcggcgc cgtggtcgag gggtcgttgg gcgtagggcc cgagaaaggt gttgtcgacg
      841 atggtgagca cccggtgccc ggtgtccgag tgctcggccg cgacacggga tgccatggcg
      901 atgtcgaaca cgtcgttggt cgggttggcc ggggtctcca cgtagatcgc gcccagccgc
      961 cggccatcga tcagagcttc gatctccgac tcggtggctc ccggcccgta tcccgccgcc
     1021 aggtaaccga gcgggcccag gaccttgtgc agcatcgtgt tggtccctcc gtacaggggt
     1081 gcgctgtgca agatgaggtc tccggggcga gcgaaggtga gcagggtggt gaagatcgcg
     1141 gacaccccac tggagaagac gagggccgat tcggcgttgt cccacaccgc gagccggccc
     1201 tcgacgatga cgagattcgg gtgatccatc cgggtgtaga tgaagtcgat ctcctccccc
     1261 tcctccgggg cagcaccgcc gtagaccacc tcgaacagac gctcggcggc ggctgaggac
     1321 tcgaagacga aggtcgacgt ctggaataga ggaggcttca gcgagttcaa gccatcggcc
     1381 ggatcccacc cccggcgcat catctccgac tcggggtggg tgacgtcccc ggagcggtct
     1441 tcggtcatgc cgtgagccta agcgcggcca tagacccccc ggacgtcgat aatcggcaca
     1501 tggagacgaa tctcgccgtt gatgtggcct acgccgtcga gatcctgcgt cccacgcgcc
     1561 ggatactcgt gttcaccggc gccgggatat ccaccgagtc ggggataccc gacttccggg
     1621 gtccggaagg actgtggacc aaggtcgatc ccgaggattt tcacatcgac cgctaccggg
     1681 ccaaccccga attgaggaag agagggtggc ggatgcacct cgacggcgag ctgtggggag
     1741 cgagatcgac agtgcgaccc aacctcgggc atgaggcgat caagcgactc gccgacagcg
     1801 gaagactctc gggagtggtc ac
//
LOCUS       DATITW010000790         1634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_398782, whole genome shotgun sequence.
ACCESSION   DATITW010000790 DATITW010000000
VERSION     DATITW010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398782"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="VLS86_11035"
     CDS             complement(<1..135)
                     /locus_tag="VLS86_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018119509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSO51072.1"
                     /translation="MIQGYRAPQVCKLVGITYRQLDYWARTNLIKPSLQQANGSGSQR
                     L"
     gene            complement(339..851)
                     /locus_tag="VLS86_11040"
     CDS             complement(339..851)
                     /locus_tag="VLS86_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HSO51073.1"
                     /translation="MPGNGSPIAMEVVGVRVELPSNQPIVLLREQEGNRYLPIWIGGS
                     EATAIATALEGVNPPRPLTHDLMRSVIDAVGAFAVRVVITEMRDSVFYADLSLDISGR
                     EVHVSSRPSDAIALAVRTGTPVFALPGVLEDAGVVFEEEADEEDEVARFREFLEEVTV
                     EDFLGGEPGD"
     gene            complement(864..1466)
                     /locus_tag="VLS86_11045"
     CDS             complement(864..1466)
                     /locus_tag="VLS86_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HSO51074.1"
                     /translation="MTSGSEPAVEAIPESEIQSRVAELGHAITVDYAGRQPLFVVVLM
                     GSIPFAADLVRRVEVPSEVDFLDLNRFGEGGRIGIAMDLWTPVTDRDVVIVEDITDTG
                     LTLSVLRKTILDRGARSVATASLVDKTRRRVTEVPIEYRGFEVGDEYLVGYGMDWQGH
                     YRNLRSIWAVLDMEAFVNDPRALVDAVGWRKPASPDSQTR"
BASE COUNT          308 a          527 c          515 g          284 t
ORIGIN      
        1 gagccgctgg gacccagagc cgttcgcctg ctgcagcgaa ggcttgatca ggttggtgcg
       61 cgcccagtag tcgagttgcc ggtaggtgat tcccaccagc ttgcagacct gtggagcgcg
      121 gtagccctgg atcatctgtc ctccgtgtta caagcgtgta actcagccgt tggcagcaag
      181 ctacccctgt ccgggaggca atgtcaacga aatctgtgga taactctgtg cagggcgggc
      241 gcggaggccg ggcaccttct cccaattggg tggcaccggg ccggatatga cccccctgga
      301 tcccaactgg gcaagggggt tttccccatc cgccgggatc agtccccggg ctcgcccccg
      361 agaaagtcct ccacggtgac ctcttcgaga aactcacgaa atctggcgac ttcgtcctct
      421 tcgtcggcct cctcttcgaa gaccacaccg gcgtcttcga ggaccccggg aagggcgaac
      481 accggggttc cggtacggac ggcaagggcg atggcatcgg agggacgtga ggagacgtgg
      541 acctcgcgcc ccgaaatgtc gagcgagaga tcggcgtaga acaccgaatc tcgcatctct
      601 gtgatcacga cccgcacggc aaacgcccct actgcgtcga tcaccgaccg catcaggtca
      661 tgggtgagag ggcggggcgg gttgacccct tcgagggccg tggcaatggc ggtggcctcc
      721 gagcctccga tccagatcgg caggtaccgg ttcccctcct gttccctgag gagcacgatc
      781 ggctgattgg acggaagctc gacccggact ccgacgacct ccatcgcgat gggtgatccg
      841 ttacccggca tgacgcgagg ttatcaccgg gtctgtgaat ccggactggc cggtttccgc
      901 cagccaaccg catcgaccaa agcccggggg tcgttgacaa aggcctccat gtccaggacg
      961 gcccaaatcg atcgaagatt tcgatagtgg ccctgccaat ccatcccgta cccgaccagg
     1021 tactcgtccc ccacttcgaa cccgcggtac tcgatgggca cctcggtgac gcggcgtcgg
     1081 gtcttgtcga ccagcgaggc ggtggcgaca ctgcgcgctc ccctgtcgag aatggtcttc
     1141 cgcagcaccg agagagtgag gccggtgtcc gtgatgtcct cgacgattac gacgtcgcga
     1201 tctgtgaccg gggtccacag atccatggcg atgccgatac gacctccctc accgaaccgg
     1261 ttgagatcga ggaagtcgac ctccgaaggc acctcgacac gtcgcaccaa gtcggctgcg
     1321 aacggaatcg atcccatcag gaccactaca aacaggggct gtcggcccgc gtaatcgacc
     1381 gtgatcgcat gccccagctc agctactcgt gactggattt cggattcggg gattgcctcc
     1441 acggcaggct cagaacccga cgtcacgctc cgaggacccg gtcgatccag accaggaggg
     1501 cgtcggcaac cgaggtccgg ccgccctgag catgccgggt cacctcgacc accggcggag
     1561 gctcgagctc gggtacgtcg cccattggag acggctcttc gagggtgagg ccttgtgcgg
     1621 cgagatggag atag
//
LOCUS       DATITW010000791         2907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_239003, whole genome shotgun sequence.
ACCESSION   DATITW010000791 DATITW010000000
VERSION     DATITW010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239003"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VLS86_11050"
     CDS             <1..631
                     /locus_tag="VLS86_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51075.1"
                     /translation="GRLIYEGPVIEIYPGPLLPNYQMTQLTEDLVDGLLTLIDEIGLP
                     EMDSEVDDSAAATVADATTEVVTFWDENGAHTYSVYGLGIDPNPSNPATAATLELVAA
                     LSEASFSADSVEYTGDRVRVISGVAQVAPDPEFEDVRPWPLDEDDPAEWTELSLGFTC
                     KTFGAEVLDEFRDATQGTQWLHPDEMMDAPPFILLVRPLHPGEPDCPVT"
     gene            complement(717..2075)
                     /locus_tag="VLS86_11055"
     CDS             complement(717..2075)
                     /locus_tag="VLS86_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HSO51076.1"
                     /translation="MVTKIMLPESELPSHWYNIVPDLPVPPPPVLHPGTHQPVGPDDL
                     APLFPMDLILQEVSGERFIEIPEEVREVYRLWRPTPMYRAHNLEKLIGGPARIYYKYE
                     GVSPAGSHKPNTAVAQAYYNAKAGVKKLSTETGAGQWGSALSFACALFGLECQVFMVR
                     ASYDQKPYRRAMIETWGSTIYPSPSDRTETGRRILEADPDTPGSLGIAISEAVEVAAT
                     NDDINYSLGSVLNHVLMHQTVIGEESLKQLALVEETADVVIGCAGGGSNFAGFAFPFV
                     REKLAGKMDPTVIAVEPAAAPSLTRGSYQFDFGDTAGMTPLVKMHTLGHDFVPDPIHA
                     GGLRYHGMAPLVSLLKDQGIIEAQSMHQSETFSKAVQFARSEGIIPGPEPTHAVAAAM
                     NEAEKAKEAGEEKVIVFNLSGHGHFDMAAYQAYFAGELIDYEHPEERIAAAMARVPVV
                     AE"
     gene            2143..>2907
                     /locus_tag="VLS86_11060"
     CDS             2143..>2907
                     /locus_tag="VLS86_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSO51077.1"
                     /translation="MTTRRLISRRLAIKEKGKAGHAVMVLGTAACANGAGEGTTSTTG
                     RPATTTSTAPPSTIAGTTSSTTTAAPPAGSSFQRVDLSFVSAYILYRAGEAALVDTGV
                     DGSADAIEASLAEVGLGWEAVGHVILTHKHPDHVGSIDDVLQRAPGAAVYAGAADIPA
                     ITSSLAPVAIGDGGTVFGLEIIGTPGHTAGHISVLDSVAGILVAGDALNGTGDGVAGP
                     DPGFSEDMAIAIDSVIKLAGLDYEVALFGHGDPVLEG"
BASE COUNT          483 a          966 c          956 g          502 t
ORIGIN      
        1 cgggcgactc atctacgagg ggccggtgat cgagatctac cccgggcctc tgctccccaa
       61 ctatcagatg actcagctca ccgaggatct ggtcgacggc ctgctcaccc tgatcgacga
      121 gatagggctt ccggagatgg actcggaggt cgacgactct gcagcggcga cggtggccga
      181 cgccacgacc gaggtcgtca ccttctggga cgagaacggc gcgcatacct actcggtcta
      241 cgggcttggg atcgacccca atccctccaa cccggcgacc gcggcgacgc tggagttggt
      301 cgccgccctc tccgaggcct ccttctcggc cgattccgtg gagtacacag gtgaccgggt
      361 acgggtgatc tctggagtcg cgcaggtcgc ccccgatccc gagttcgagg acgtcagacc
      421 ctggccgctc gacgaggacg atccggcgga gtggaccgag ttgagtctcg ggttcacctg
      481 caagaccttt ggcgccgaag tcctcgacga gttccgggac gccacccagg ggacccagtg
      541 gctgcacccc gacgagatga tggacgcccc acccttcatc ctcctggtcc gccccctcca
      601 ccccggagag cctgactgcc ccgtgacgta gcaccaccct ctcgtaattg ggacggggag
      661 gggtcaaata cggacctgtg gatcccaatt tggcggtccc aaccgggacg gcgggtctac
      721 tcggcgacca ccggaacccg ggccatggcg gcggcgatgc gctcctcggg gtgctcgtag
      781 tcgatcagct ccccggcgaa gtaggcctga taggcggcca tgtcgaagtg cccgtgcccg
      841 gacaggttga acacgatcac cttctcctcc cccgcttcct tcgccttctc ggcctcgttc
      901 atcgccgcgg cgacggcgtg ggtcggctcg ggcccgggga tgatcccttc cgacctggcg
      961 aactggacag ccttgctgaa ggtctcggac tgatgcatgg actgggcctc gatgatcccc
     1021 tggtccttga gcagtgacac caatggcgcc atcccgtgat agcggagccc gccggcgtgg
     1081 atcggatcgg ggacgaaatc gtgtcccagc gtgtgcatct tgaccagtgg cgtcatcccg
     1141 gccgtgtcgc cgaaatcgaa ctggtagctg cccctggtca gcgacggcgc cgcggcgggt
     1201 tcgactgcga tgaccgtggg gtccatcttc cctgcgagct tctcccttac gaacgggaac
     1261 gcgaagccgg cgaagttcga tcctccaccg gcacagccga tcacgacgtc ggctgtctcc
     1321 tcgaccaggg ccagctgctt caacgactcc tccccgatga cggtctggtg catcagcacg
     1381 tggttgagca ccgaccccaa cgagtagttg atgtcgtcgt tggtggccgc aacctcgact
     1441 gcctcggaga tggctatccc gagactcccc ggggtgtcgg ggtccgcctc caggatcctg
     1501 cgcccggtct cggtccggtc cgacggtgag ggatagatgg tcgagcccca ggtctcgatc
     1561 atggccctgc ggtacggctt ctggtcgtag gaagcgcgca ccatgaacac ctgacattcc
     1621 agaccgaaca gggcacaggc aaaggagagc gccgagcccc actgacctgc tcctgtctcg
     1681 gtggagagct tcttcacccc ggccttggcg ttgtaatagg cctgagccac cgcggtgttg
     1741 ggcttgtgag agcccgccgg gctcacccct tcgtacttgt agtagatccg ggccggtccc
     1801 ccgatcagct tctccaggtt gtgggcccgg tacatcggcg tcgggcgcca cagccgatag
     1861 acctcacgca cctcttcggg gatctcgatg aaacgctctc cactgacctc ctggaggatc
     1921 agatccatcg ggaacaatgg ggcgagatcg tcggggccga ccggctgatg ggtgcccggg
     1981 tgcagaacgg gtggtggtgg gaccgggagg tcggggacga tgttgtacca gtgggatgga
     2041 agctcggatt cgggaagcat gatcttggta accatccgcc cgaagctaca cacaatcccg
     2101 gggggcaggt aggttgcact gttcccaacc gggagcagac tcttgaccac ccgccgtctc
     2161 atcagccgtc gcctcgccat caaggagaag ggcaaggcag gtcacgcggt gatggtcctc
     2221 ggaaccgcgg cctgtgccaa cggcgccggg gaggggacca catccaccac gggccgaccg
     2281 gcgacgacca cctcgacggc cccaccgtcg accatcgccg gcaccacgtc gagcaccacc
     2341 accgccgcgc cgcccgccgg ttcgtccttt cagcgtgtcg atctgagctt tgtctccgcc
     2401 tacatccttt accgggccgg tgaggcggct ctcgtcgaca ccggggtcga cgggagcgcc
     2461 gacgccatcg aggcgtcgtt ggccgaggtg ggacttggct gggaggcggt cggccatgtg
     2521 atcctcaccc acaagcatcc cgaccacgtg gggagcatcg acgatgtgct gcagcgggcg
     2581 ccaggagccg cggtatacgc gggagccgcc gatatacccg cgattacctc gtcgctggcc
     2641 ccggtggcga tcggagacgg agggacggtg ttcggcctcg agataatcgg gacccccggg
     2701 cacaccgcgg gtcacatctc ggttctggac agcgtcgccg ggatcctggt ggcaggggat
     2761 gcgctcaacg ggaccggcga cggggtcgcc gggccggatc ccgggttcag cgaggacatg
     2821 gccatcgcca tcgactcggt gataaagctc gccgggctcg actacgaggt tgccctgttc
     2881 gggcacggcg atccggtgct cgagggt
//
LOCUS       DATITW010000792         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_198568, whole genome shotgun sequence.
ACCESSION   DATITW010000792 DATITW010000000
VERSION     DATITW010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198568"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VLS86_11065"
     CDS             complement(<1..565)
                     /locus_tag="VLS86_11065"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSO51078.1"
                     /translation="MAATRAASRYSAPKVRVALIGLFLVAAWLGMAYRLVQVQVVQAA
                     DLAGQGLTQRLVTRDLAPQRGKIFDRNGELLAMTVEAQSVWANPGQIEEPLWVAQQVG
                     GLLGVDSEVLYERLTSERDFVYLKRQVEPVMAEQIASLEVRGLYTHPEATRIYPAGQV
                     AAHVTGFVDIDGNGQEGLELVYEDQLRG"
     gene            complement(646..1032)
                     /locus_tag="VLS86_11070"
     CDS             complement(646..1032)
                     /locus_tag="VLS86_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51079.1"
                     /translation="MTALPLRGTASEERLRVLKGRRLQRLTLAPWMIMVLIGVVAFLG
                     LGFARTSLDKSAFELDQLNRAITEQGAINEQLRLEIARLENPARIAPLAEEMGLVIPG
                     DTRQLLVDLAPRGPAVASVESEGETQ"
     gene            complement(1029..1964)
                     /gene="rsmH"
                     /locus_tag="VLS86_11075"
     CDS             complement(1029..1964)
                     /gene="rsmH"
                     /locus_tag="VLS86_11075"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HSO51080.1"
                     /translation="MSQSRNNRDRDYHRPVLEQEVVDLLAPVLPGVVVDATLGGGGHS
                     RRLLDEFGEDVTVVGIDRDPEALANARMLGVRAVQGNFADLADVVRAATDQPISGILF
                     DFGVSSHQLDQASRGFSYRHEGPLDMRMGPDAAHTAAEIVNEWPESRLTEIIRRYGEE
                     PLARRIAQAIVAGRPYQTTSQLSTVIAGAMPAARRRAGHPARRTFQALRIAVNDELDA
                     ISSGLDQAIEIVRPQGRILAISYHSLEDRIVKRRFTRGATGCVCPPDLPVCGCGQTAE
                     LRILTRKPIRASDAEIEANNRARSAVLRAAEKVAA"
     gene            complement(2063..2476)
                     /locus_tag="VLS86_11080"
     CDS             complement(2063..2476)
                     /locus_tag="VLS86_11080"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HSO51081.1"
                     /translation="MFLGEYFHSLDEKGRVVMPSGFRRRLEDGCVVAKGQDGQLVLFP
                     TGEFEARAAEVREMPQDRPGRRFSRTVFSGADMQMVDKSGRVLVKPDLRKFADLNAST
                     EIAVIGVFDHVELWNMERYLADRAAGDESYLEEGE"
BASE COUNT          437 a          910 c          820 g          467 t
ORIGIN      
        1 caccgcggag ctggtcttcg tagaccaact cgagaccctc ctggccgttg ccgtcgatgt
       61 cgacaaaccc ggtgacatga gccgcaacct gaccggcggg gtagatccgt gttgcctcag
      121 ggtgggtgta cagcccgcga acctcgagtg atgcgatctg ctccgccatg accggctcca
      181 cctgacgctt caggtagacg aagtcccgct ccgaggtgag gcgctcgtag aggacctcgg
      241 agtcgacacc gagcaggcct ccgacctgtt gtgccaccca gagtggctcc tcgatctggc
      301 ccgggttggc ccagacactc tgcgcctcga ccgtcatcgc cagcagctcg ccgttgcgat
      361 cgaagatctt tccccgctgc ggggccaggt cgcgggtgac gagtcgctgg gtcagaccct
      421 ggccggccag gtcggcggcc tgaaccacct gcacctgaac cagtcgatag gccatgccca
      481 gccatgccgc aaccaggaac aggccgatca gagccacgcg taccttgggc gcggagtaac
      541 ggctggcggc ccgggtggca gccacgacct atgtccccgg ccgtgactcc ggaggttcgg
      601 ggcgacctgc cccgtccgtg agcccggctc cagcgcgccg tcgggtcatt gtgtttcgcc
      661 ttcgctttcg accgaggcca ccgccggccc ccgaggtgcg agatcgacca ggagctgcct
      721 ggtgtcaccc gggatcacca accccatctc ctccgccaga ggcgcgattc gggccggatt
      781 ctccagacgg gcgatctcca gtcgtagctg ctcgttgatc gccccctgct cggtgatcgc
      841 ccggttcaac tggtccagct cgaacgccga cttgtccagc gaggtcctgg cgaaacccaa
      901 tcccaggaaa gccaccactc cgatcaggac catgatcatc cacggcgcca gtgtcagccg
      961 ctgcagtcgt cgccctttga ggacccggag tctctcctcg ctcgccgtgc cccgcagtgg
     1021 tagggccgtc atgccgccac cttctccgcc gcgcgcagga ccgcggagcg agccctgttg
     1081 ttcgcctcga tctccgcgtc ggaggctcgt atcggcttcc gggtgaggat ccggagctcg
     1141 gcggtctggc cacagccaca caccggcagg tcgggggggc agacgcatcc tgtggccccc
     1201 cgggtgaaac gcctcttgac gattcgatcc tcgagcgagt ggtaggagat ggcgaggatc
     1261 ctgccctgag gcctcacgat ctcgatcgcc tgatccagcc cgctgcttat ggcgtccagc
     1321 tcgtcgttga ccgcgatccg gagcgcctga aaggtccgtc gggcgggatg acccgcccga
     1381 cggcgagccg cgggcatggc cccggcaatg acggtggaaa gctggctggt ggtctgatag
     1441 gggcgaccgg cgacgatcgc ctgtgcgatg cgacgggcga gaggctcctc gccataacgc
     1501 cggattatct cggtgagacg actctcgggc cattcgttga cgatctcggc agcggtatgg
     1561 gcggcgtcgg gccccatacg catgtcgagt ggcccctcgt gacgatagga gaagcctcgc
     1621 gatgcctgat ccagctggtg cgaggagacc ccgaagtcga agaggatccc ggagatcggc
     1681 tggtcggtcg ccgccctgac cacgtcggcg agatcggcga aattcccctg aacggcgcga
     1741 acgccgagca tcctggcgtt ggccagggcc tcaggatcgc ggtctattcc cacaactgtc
     1801 acgtcttctc cgaactcatc cagcaaccga cgactgtgtc ccccgccacc cagagtcgcg
     1861 tccaccacaa ctccgggcag caccggggcc aacagatcga ccacctcctg ctccagaacc
     1921 ggccggtgat agtcccggtc tcgattgttc cggctctggc tcatggccag ccccccgggc
     1981 gctggtacca ataggaagcg ggcggagtca ggggccttcc attggttccg gggggctggc
     2041 gatgaaccag ccacgtgctg ggttattcgc cctcctcgag gtagctctcg tcacccgcgg
     2101 ctcgatcggc caggtaacgc tccatgttcc acagctccac atggtcgaag accccgatca
     2161 cggcgatctc ggtggaggcg ttgagatcgg cgaacttgcg cagatcgggc ttgaccagga
     2221 cccggcccga cttgtccacc atctgcatgt cggcgccgga gaacacggtg cgcgagaatc
     2281 ggcgaccggg gcggtcctgc ggcatctctc tcacttccgc cgcccttgcc tcgaactcac
     2341 cggtcgggaa gagaacgagc tgaccgtcct ggcccttggc aacgacacaa ccgtcttcga
     2401 gacgacggcg aaaaccggag ggcatgacca ctcggccctt ctcgtcgagg ctgtggaagt
     2461 actcaccgag gaacacttat gttcttctcc gtccgttctc attgcttgga ccccttctcg
     2521 ctggggtgct gtccttgcgg gactgttcgc cactatgacc cactttgccc cacttgtcaa
     2581 ccttttcccc cactaggacc catccggtga cccacgggca ggatcgagga tctc
//
LOCUS       DATITW010000793         1958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_198664, whole genome shotgun sequence.
ACCESSION   DATITW010000793 DATITW010000000
VERSION     DATITW010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198664"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..>1958
                     /locus_tag="VLS86_11085"
     CDS             17..>1958
                     /locus_tag="VLS86_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSO51082.1"
                     /translation="MTATQESPEVRALDEIKIGGMGASRKRVEDNRFIRGKGNYVDDI
                     VLPGMLHMEILRSPLAHARIKSIDTSKAYAIPGVRLVLTGDMMATRNLAWMPTLSYDT
                     QAVLATDKVRFQGQEVACVIADDPYIAKDACEAILVDYEPLPPIVNPTQSLAEGAPLI
                     RDDKENQPDNVIYDWEVGDKNGTDQAFAQADLVSKIDLFYPRSHPSPIETCGSIADFN
                     RSTSKLTVYMTTQAPHIIRAAVALVAELPEQMIRIIAPDIGGGFGNKVPVYPGYVASI
                     LASILLEKPVKWIEDRTGNLISTGFARDVYLHGELALRKDGRILGVRMQTIADHGAFF
                     SDAQPSKFKIGLMHSAFACYDIPNAHLVARGVYTNKAPGGVAYRCSFRVTEAMFFQER
                     MVQAAADDLGMDQAEFRRINFVRDDDFPHRTPWGFLTDSGQYAKCLDVGLEAIGYQDF
                     LKQKEEAAKRGRLLGIGISTMTEPLGAGNSREYDILGIKMFDSAELKVHITGKALLRT
                     GAKSQGQGHETTWAQIVAHELGIPAEDIFVEEGDTDTAPFGMGTSASRSTPVAGAAVA
                     MVSRKIRAKARTIAAHLLEISEEDVEWELGRFYVRSQPDRGVTIQECAVAAYSNMPDG
                     MEPGLDNTAYYDPPNLTWPFACY"
BASE COUNT          374 a          671 c          611 g          302 t
ORIGIN      
        1 aggagagtgc gcagccgtga cggccacgca ggagagcccg gaggttcgcg ctctcgacga
       61 gatcaagatc ggcgggatgg gggccagccg caagcgggtc gaggacaacc ggttcatccg
      121 cggcaagggc aactacgtcg acgacatcgt gctgcccggc atgctgcaca tggagatcct
      181 gcgcagcccc ctggcgcacg cgcggatcaa gtccatcgac accagcaagg cctacgccat
      241 ccccggcgta cggctggtgc tcaccggcga catgatggcc acccgcaacc tggcctggat
      301 gccgaccctc tcctacgaca cccaagcggt gctggccacc gacaaggtcc gattccaggg
      361 acaggaagtc gcatgcgtca tcgccgatga cccctacatc gccaaggacg cctgcgaggc
      421 gatcctggtg gactacgagc cgcttccgcc gatagtcaac ccgacccagt cgctagccga
      481 aggggcgccg ttgatccggg atgacaagga gaaccagccc gacaacgtca tctacgactg
      541 ggaggtgggc gacaagaacg ggaccgacca ggccttcgcc caggccgacc tcgtctccaa
      601 gatcgatctc ttctaccccc gctcccaccc ctcgccgatc gagacctgcg ggtcgatcgc
      661 cgacttcaac cgctccactt cgaagctcac ggtctacatg accacccagg cgccccacat
      721 catcagggcg gcggtggcat tggtggccga gctccccgag cagatgatcc ggatcatcgc
      781 cccggacatc ggcggtgggt tcggcaacaa ggtgccggtc taccccgggt acgtggcgtc
      841 gatcctggct tcgatcctcc tggagaagcc ggtgaaatgg atcgaggacc gcaccggaaa
      901 cctgatctcc accggcttcg cccgcgatgt ctatctccat ggtgagctgg cgttgcgcaa
      961 ggacgggcgg atcctgggag tgcggatgca gaccattgcc gaccacggcg cgttcttctc
     1021 cgacgcccag ccttccaagt tcaagatcgg gctgatgcat tcggcgttcg cctgttacga
     1081 catccccaac gcccatctgg tggcacgcgg ggtgtacacc aacaaggcgc ccggtggcgt
     1141 cgcctatcgg tgctcgttcc gggtcaccga ggcgatgttc ttccaggagc gaatggtcca
     1201 ggcggcggcc gacgacctgg ggatggatca agccgagttc cgccggatca acttcgtccg
     1261 cgatgacgac ttcccccacc gcaccccatg ggggttcctc accgactcgg gccagtacgc
     1321 caagtgcctc gacgtcggtc tggaggcaat cggctaccag gatttcctca agcagaagga
     1381 agaggccgcc aagcgcggcc gtctgctggg catcgggatt tccacgatga ccgagccgtt
     1441 gggcgccggg aacagccggg aatacgacat cctcgggatc aaaatgttcg actcggccga
     1501 gttgaaggtc cacatcacgg gcaaggctct gctccgcacc ggggccaaga gccagggcca
     1561 ggggcacgag accacctggg cccagatcgt ggcccacgag ctcggcatcc cggccgagga
     1621 catcttcgtc gaggaggggg acaccgacac cgccccattt ggaatgggca cctctgcctc
     1681 ccgaagcacc ccggtggcag gcgcggccgt ggccatggtc tcccgcaaaa tccgggccaa
     1741 ggcccgcacg atcgccgctc atctgctcga gatctcggag gaagacgtcg agtgggagct
     1801 ggggcgcttc tacgtgcgca gccagcccga ccggggcgtg accatccagg agtgcgccgt
     1861 tgccgcctac tccaacatgc ccgacggcat ggagccgggt ctggacaaca cggcttacta
     1921 cgacccgccc aacctcacct ggccgttcgc gtgctaca
//
LOCUS       DATITW010000794         1575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_39086, whole genome shotgun sequence.
ACCESSION   DATITW010000794 DATITW010000000
VERSION     DATITW010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39086"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VLS86_11090"
     CDS             complement(<1..402)
                     /locus_tag="VLS86_11090"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HSO51083.1"
                     /translation="MRIVLVTGKGGVGKTTVAASTALAAADAGYRTLVTSTDPAHSLS
                     DALMIGLGSDPSKVVPGLDGQQIDTQRQLDKYWGSIRRQLMDVLDWGGAGGIEAEEFF
                     VFPGMDELFALLDVNRHARSGVYDVIVVDCAP"
     gene            complement(407..844)
                     /locus_tag="VLS86_11095"
     CDS             complement(407..844)
                     /locus_tag="VLS86_11095"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSO51084.1"
                     /translation="MAEGTFQTLEIDASPEVLYDAAADIASYPEWATGVKEVEVLESG
                     PDGRVDRARFVIEVFVKEIEYVLRYEHDRPNRLSWVAEESDDLEMLEGSYTFTPNNGA
                     TEVLYGLKVGLKFTMPGFLMRQAEKQIVTTALRGLRKRVTGSG"
     gene            complement(890..>1575)
                     /locus_tag="VLS86_11100"
     CDS             complement(890..>1575)
                     /locus_tag="VLS86_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSO51085.1"
                     /translation="VNLTDYRTGEGAVADVLGLDFARASATARAAGDYQGMRDLWSGQ
                     AAGRFDEVRAEIGEAIDSQYQEVALALQGGSGYVIHGNVDRPASLRASLPAGFEYVHG
                     QVVEIEGIRFGFAGGGAPTPLQAEGEVSDDEMRLLLEHIGPVDVLCTHVPAAVHPLRT
                     DVITGRAERGSAPILEYLIREKPRVHLFGDVHQPQATTWRLGATRCRNVGYFRATGRP
                     VRIDRSWIE"
BASE COUNT          275 a          535 c          494 g          271 t
ORIGIN      
        1 cggggcgcag tcgaccacga tcacgtcgta gaccccggat cgggcgtggc gattgacgtc
       61 gagcagggcg aacagctcgt ccatcccggg gaacacaaag aactcctcgg cttcgatccc
      121 accggcgcct ccccaatcca gcacgtccat cagctggcgg cggatcgagc cccagtactt
      181 gtcgagctgt cgctgggtgt cgatctgctg cccgtcgaga ccggggacga ccttcgacgg
      241 atcgctgccg agaccgatca tcaaggcgtc ggacagagag tgcgcggggt cggtcgaggt
      301 gaccagggtg cgatatccgg cgtcggccgc agcgagggcg gtggaggcgg ccaccgtggt
      361 cttgcccacc cctcccttgc cggtgacgag gactattcgc atgatctcag cccgagccgg
      421 tgactctctt gcgaagcccc cgaagcgcag tggtcacgat ctgcttctcg gcctgacgca
      481 tgaggaaacc cggcatcgtg aacttgaggc cgaccttcaa gccgtagagg acctcggtgg
      541 cgccgttgtt gggtgtgaag gtgtaggagc cttcgagcat ctccaggtcg tcgctctcct
      601 cggccaccca gctcaaccgg ttcggccggt cgtgctcgta gcggaggacg tactcgatct
      661 cctttacgaa gacctcgatc acgaaccgtg cccgatcgac ccggccgtca gggccgctct
      721 cgagaacctc cacctccttc accccggtgg cccactccgg gtaggaggcg atgtcggcgg
      781 cggcgtcata gagcacctcg ggtgaggcgt ctatctcaag agtctgaaag gttccctcgg
      841 ccatcgctca cagtcctcct ccggcagacg agactagcga gacatcggcc tattcgatcc
      901 aggaccggtc gatgcggacc ggccgcccgg tggcccggaa gtacccgaca ttgcgacacc
      961 gggtagcccc gagacgccag gtggtcgcct ggggctgatg cacgtcaccg aagagatgga
     1021 cccgaggctt ttcccgaatc agatactcga ggattggcgc cgagccgcgt tccgcccttc
     1081 cggtgatgac atctgtgcgc agcggatgga ccgccgccgg gacatgggtg cagaggacat
     1141 cgaccggacc gatgtgctcg agcaacagcc tcatttcgtc atcagagacc tctccttcgg
     1201 cctgcaaagg cgtcggcgcc ccgccaccgg cgaaaccaaa ccgaatcccc tcgatctcga
     1261 ccacctgacc gtggacatac tcgaatcctg cgggcaggct ggctcggaga ctcgccgggc
     1321 gatcgacatt cccatggatg acgtacccgc tccccccttg caatgcgagg gccacctcct
     1381 gatattggga gtcgatcgcc tcaccgatct ccgcccggac ctcgtcgaat ctcccggcgg
     1441 cctgccccga ccacaggtct ctcattccct ggtagtcacc ggcggcgcgg gctgtggcgg
     1501 aagctcgggc gaagtcgagg ccgaggacgt cggccactgc cccctccccg gttcggtagt
     1561 cggtgaggtt gacca
//
LOCUS       DATITW010000795         3438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_639969, whole genome shotgun sequence.
ACCESSION   DATITW010000795 DATITW010000000
VERSION     DATITW010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639969"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VLS86_11105"
     CDS             complement(<1..876)
                     /locus_tag="VLS86_11105"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HSO51086.1"
                     /translation="MAHILGFGGYVPERVMTNDDWAALLDTSDEWITQRTGIKRRRVA
                     AADETTLTLACHAASMAIKDAGLAPDDIDEIIVATDTPEVYLPDTASFVQHRLGARHV
                     PAYDLGGSGCAGFLQALDVACSRVRSGKERVLVVGVEVLTRLMNWQDRNTCVLFGDAA
                     GAAVVGRGPEAREVLAAVAGTDGSRWDILTLETGGTRLPFSLEGARQDLHHRIVMKGR
                     EVFKEAVSRMSEAAREVLAKAGRQMEEVALVIPHQANLRIIEAVGKALGVAPERVFVN
                     VQDYGNTGSASVPLAL"
     gene            complement(922..1260)
                     /locus_tag="VLS86_11110"
     CDS             complement(922..1260)
                     /locus_tag="VLS86_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006517382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSO51087.1"
                     /translation="MTEFRYTSWFAAEAAGADQGDPYAVMLRRGTLELGFYAPIGVDR
                     QEPHEQDEVYVIVSGTGRFLNGGVETEFGPGDALFVPAGVEHRFIDFSDDTEMWVVFY
                     GPPGGEARDI"
     gene            complement(1265..2356)
                     /locus_tag="VLS86_11115"
     CDS             complement(1265..2356)
                     /locus_tag="VLS86_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HSO51088.1"
                     /translation="MSDTPSRRARLRQLGIGIGALETGAENAISDVPGVLVGHATVIR
                     DHPVPVRTGITMIVPRAGEIWTDYAYAGSHVLNGNGEMTGLTWVEESGMLGSPVGITS
                     TAQVGLVRDSLVKESYRLGVFEGFHLPVVGETWDGWLSTAESFPLTDAEVAESLGSAA
                     AGPVAEGSVGGGTGMICHEFKSGIGTSSRLVEGGGERHTVGVLVQANYGAREDLRVDG
                     APVGRLIGPDIVPSAWDKPPTGGSIIAVIATDAPLLPGQCRRLAQRATVGLARVGGYG
                     HDSSGDIFFAFSTGNHLMHGESSARSVRSLPNEEIDGLFHAVADATEESIVNALCSAE
                     TMIGREGRTAHALPLERMVDIVDQHRAGK"
     gene            2566..>3438
                     /locus_tag="VLS86_11120"
     CDS             2566..>3438
                     /locus_tag="VLS86_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSO51089.1"
                     /translation="MSARGAWITDARGKMYLDAAGGAIVVGIGHGDPDVAAALTSQAS
                     ALDWVHASAFTTEPVEDYAAALASLVPLDEARVFPVSGGSEAMETALKMARAYHVARG
                     EVERDVIIARAHSYHGNTRGALDVSGREPLRRPYQPWLGQTARVPGVSEYRCPNPGHP
                     EACAVFHATTLEQTILELGPQRVAAFVAEPVGGAASGAALPPDGYWDEIARVCRRHGV
                     LIIADEVMTGFGRTGEWFASDHFGLRPDILVAAKGASSGYWPLGLAVTTGPVHDTIMS
                     GGGLVHGLTWSHHPM"
BASE COUNT          520 a         1207 c         1157 g          554 t
ORIGIN      
        1 cagggccagc ggcaccgagg ccgagccggt gttgccgtag tcctggacgt tgacgaacac
       61 ccgctcgggc gccacgccca gggccttgcc caccgcctcg atgatccgca ggttggcctg
      121 gtgggggatg acgagggcga cctcctccat ctggcgtccg gccttggcca gcacctcccg
      181 cgccgcctcg ctcatgcgcg acacggcctc tttgaacacc tcgcggccct tcatcacgat
      241 gcggtggtgg aggtcctggc gcgccccctc gaggctgaag gggagccgcg tgccgcccgt
      301 ctccagggtg aggatgtccc agcggctgcc gtcggtgccg gcgacggcgg cgagcacctc
      361 acgggcctcg gggccgcggc ccaccaccgc cgctcccgcc gcgtcgccga acagcacgca
      421 ggtgttgcgg tcctgccagt tcatgaggcg ggtgagcacc tcgacgccca ccaccagcac
      481 ccgctccttg cccgagcgca cccgcgagca ggccacgtcg agggcctgga ggaacccggc
      541 gcagccgctg ccgcccaggt cgtaggccgg gacgtggcgc gcccccaggc ggtgctggac
      601 gaacgaggcg gtgtccggca ggtagacctc cggggtgtcg gtggcgacga tgatctcgtc
      661 gatgtcgtcg ggtgccagcc cggcgtcttt gatcgccatg ctcgccgcgt gacaggccag
      721 ggtgagcgtc gtctcgtcgg ctgctgcgac acgacgtcgc ttgatcccgg tccgctgggt
      781 gatccactcg tcactggtgt cgagcagtgc agcccagtcg tcgttggtca tcaccctctc
      841 gggcacgtag ccaccaaagc ccaggatgtg tgccattgcg gggaggttag ggcccgctct
      901 cggcggttgc gcgagcaggt ctcagatgtc gcgagcctcg ccgcccggag ggccgtagaa
      961 gacgacccac atctcggtgt cgtcgctgaa gtcgatgaag cgatgctcca ccccggcggg
     1021 gacgaacaag gcatctccgg ggccgaactc ggtttcgacc ccaccgttga ggaaccggcc
     1081 ggtgcccgag acgatcacgt agacctcgtc ctgctcgtgc ggctcttggc gatcgacccc
     1141 gatgggggcg tagaagccca gctcgagggt cccccggcgc agcatcaccg catacggatc
     1201 accctggtcg gccccggccg cctcggcggc gaaccacgac gtgtatctga actcggtcaa
     1261 agcctcactt ccccgctctg tgctgatcga cgatatcgac cattcgctcc agaggcagag
     1321 catgagccgt ccggccttcc ctgccgatca tcgtctccgc cgagcagagg gcgttgacga
     1381 tcgattcctc ggtggcatcc gccaccgcat ggaacaagcc atctatctcc tcgttgggga
     1441 gtgacctcac cgagcgcgcc gagctctcac cgtgcatcag gtgatttcct gtggagaagg
     1501 cgaagaagat gtcgccactc gagtcgtgac cgtatccacc gactcgcgcc aggccgacgg
     1561 tggcgcgttg cgccagacgt cggcactgcc cgggtagcag cggggcatcg gtggcgatga
     1621 ccgcgatgat cgagcccccg gtgggaggtt tgtcccatgc cgaggggacg atgtcgggcc
     1681 cgatcaaccg gcctactgga gccccgtcca cccgcaggtc ctcgcgcgcc ccatagttgg
     1741 cctggaccag cacgccgacc gtgtgacgct cgcccccacc ctcgaccagg cgcgaggagg
     1801 tgccgatccc ggacttgaac tcgtgacaga tcatgccggt gccacccccg accgagccct
     1861 cggccaccgg gccggccgcg gcggatccga gcgactccgc cacctcggca tcggtgagtg
     1921 ggaacgactc cgcggtgctc agccacccat cccacgtctc cccgaccacg gggagatgga
     1981 aaccttcgaa taccccgagc cggtaggact ccttgaccag agagtcgcgc accaatccaa
     2041 cctgagccgt gctggtgatg ccgaccgggg agccgagcat ccccgactct tcgacccagg
     2101 tgagaccggt catctccccg ttcccgttga ggacgtgact gcctgcgtag gcgtaatcgg
     2161 tccatatctc acctgcccgg ggcacgatca tggtgatccc ggtgcggacc ggcaccggat
     2221 ggtcccggat cacggtggca tgaccaacca ggacacccgg cacatcgctg atcgcgttct
     2281 ccgccccggt ctccagagcc ccgatcccga tcccgagctg acgcagcctg gctctccggg
     2341 acggggtgtc tgacatcgtc cggacggtac ccgggggcgt atcgtcctgc cctccatgag
     2401 agagacggat cacgtggcga gggcagagga gcagagggaa gcgggtgtca cccccggccc
     2461 cggttcgctg gcggccgagc gtcgtgctgt gcccggggac aacccccgga tgggcccgcc
     2521 gacttgagct ctgtcttcgc cagaggcgtc gatttgccgc ttgcggtgtc tgcccgaggc
     2581 gcctggataa ccgacgccag ggggaagatg tacctcgacg ctgcgggtgg ggcgatcgtg
     2641 gtcgggatcg gccatggcga cccggatgtg gccgcggccc tgacctccca ggcctccgcc
     2701 ctggactggg tgcatgcgtc cgccttcacc accgagccgg tcgaggatta cgcggccgcc
     2761 cttgcctcgc tggtcccgct cgacgaggcc agggtcttcc cggtttccgg tgggtcagag
     2821 gcgatggaga ccgctctcaa gatggccagg gcctatcacg tggcgcgggg ggaagtggag
     2881 cgggacgtga tcatcgcccg cgcccactcc tatcacggga acacccgcgg agcgctcgac
     2941 gtctcgggcc gtgagccctt gcgaaggccc taccagccct ggctcgggca gacggccagg
     3001 gtcccggggg tgagcgagta tcggtgcccc aacccgggtc atcccgaggc ttgcgccgtg
     3061 ttccatgcca ccaccctgga gcagacgatc ctggagttgg gcccgcaacg ggtggccgcc
     3121 ttcgtggccg agccggtcgg tggtgccgcc agtggggcgg ccctccctcc cgacggctac
     3181 tgggatgaga tcgcccgggt gtgccgcaga catggcgtcc tcatcatcgc ggatgaggtg
     3241 atgaccggct tcgggcgcac cggtgagtgg ttcgcttcgg atcacttcgg tctccggccc
     3301 gacatcctgg tcgcggccaa aggagcctcc tccggctact ggccattggg tctggccgtg
     3361 acgacggggc cggttcacga caccatcatg agtggtggcg gactggtcca tgggctcacc
     3421 tggtcccacc acccgatg
//
LOCUS       DATITW010000796         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_239295, whole genome shotgun sequence.
ACCESSION   DATITW010000796 DATITW010000000
VERSION     DATITW010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239295"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2477)
                     /gene="acnA"
                     /locus_tag="VLS86_11125"
     CDS             complement(<1..2477)
                     /gene="acnA"
                     /locus_tag="VLS86_11125"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HSO51090.1"
                     /translation="MFIDPFGSRATLDTALGSRDIVRLDAIEGVERLPYSIKVLLESA
                     LRNLDGLSVHENDVRRIAAYDATNVGEQEIPFVPGRVVLQDFTGVPAVVDLAAMRSAI
                     VRLTGDPGAAQRVNPLIPADLVVDHSVQVDAFARSDALVINSRKEFERNEERYEFLKW
                     GQSGFANFRVVPPATGIVHQVNLEYLAKVAWDDGQWLYPDSLVGTDSHTTMINGLGVV
                     GWGVGGIEAEAAMVGQPIHMLLPEVVGFRLTERLPEGATATDLVLRVTEILREHGVVD
                     KFVEFHGPGLADMPVANRATIANMAPEYGATVGFFPVDEQTLKYLRLTGRDDKLIDAV
                     EQYYKLQGMWRDDSRAITYSAELELDMSTVEPSLAGPRRPQDRIALSTMEPQWKSDLT
                     NLFGKNGGAKTSTVVSWEDEGGSPAPTGVLKAKVGVDVVYDGDEFKLSDGDVVIAAIT
                     SCTNTSNPDVMVGAGLVARKARALGLHRQPWVKSSLAPGSKVVTEYLTSAGLLDDLES
                     LGFYIVGYGCTTCIGNSGPLPEPISEAISANDLVAVSVLSGNRNFEGRISPDVRANYL
                     ASPPLVVAYAIAGTVDWDPVNEPIGQDRNGNDVFLRDIWPTQEEIALIVASHVNRGQF
                     VDKYSDVFTGSDEWREIVTTESDLYSWDEGSTYIQEPPFFVALTPEITPITPVTGARA
                     LAKLGDSVTTDHISPAGSIAATSPAGRYLISKGVQPPSFNSYGSRRGNDRVMTRGTFA
                     NIRVRNQLAPGTEGGWTTDFIDGEVKSIYDAAIHYQEAGIPLVVLAGKDYGMGSSRDW
                     AAKGTFLLGVKAVLTESFERIHRSNLV"
     gene            complement(2618..>3120)
                     /locus_tag="VLS86_11130"
     CDS             complement(2618..>3120)
                     /locus_tag="VLS86_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51091.1"
                     /translation="RPSGKGGPGAGRPAKCYEASAKEVSVHFAPRRFEMLVEMLIRIL
                     DRVGPENLSEVAESVGRAYGQEIAAEIGVPEQPGYDGAVQAVASAMTGLGFSVDPDIG
                     GQRLLTSYCPFGEAATSHPEVICSLDRGIVAGMFGALSYACDPVIIPHTVLEEACVTR
                     VPVTIG"
BASE COUNT          529 a         1005 c         1035 g          551 t
ORIGIN      
        1 atcaccaggt tggagcggtg gatcctctcg aacgactcgg tcagcacagc cttcaccccg
       61 aggagaaaag tccccttcgc cgcccaatcg cgtgacgagc ccatcccgta gtccttccct
      121 gccaggacga cgaggggaat cccggcctcc tgatagtgga tcgccgcgtc gtagatcgac
      181 ttcacctcgc catcgatgaa gtcggtcgtc cacccgccct cggtgcccgg ggcgagttgg
      241 ttgcggaccc ggatgttggc gaaagtgccg cgggtcatca cccggtcgtt gccacgacgt
      301 gagccgtagg aattgaacga cggtggttgg acccccttgg atatgagata cctgccggcg
      361 gggctggtcg ccgcgatcga gcctgccggg ctgatgtggt cggtggtcac cgagtcaccg
      421 agcttggcca gggccctggc gccggtcacc ggcgtgatcg gcgtgatctc gggggtcagg
      481 gccacgaaga agggtggctc ctggatgtag gtcgatccct cgtcccagga gtagagatct
      541 gattccgtgg tcacgatctc ccgccattcg tcggatccgg tgaagacatc ggagtacttg
      601 tccacgaact ggccccgatt gacgtgggag gcgacgatca gggcgatctc ctcctgggtc
      661 ggccagatgt cgcggaggaa cacgtcattc ccgtttcggt cctggccgat cggctcgttg
      721 accgggtccc agtccaccgt ccccgcgatc gcataggcca cgaccagcgg tggcgaggcg
      781 aggtagttgg cccggacgtc gggggagatc cgaccttcga agttccggtt gcccgaaagc
      841 acggacaccg cgaccaggtc attggcactg atcgcctccg agatcggctc cgggagcggg
      901 cccgagtttc cgatgcacgt cgtgcacccg tagccgacga tgtagaagcc aagcgactcg
      961 aggtcgtcga ggagaccggc gctggtgagg tactcggtga ccaccttgga ccctggagca
     1021 agggaggact tgacccaggg ctggcggtgg agacccaatg cccgtgcctt gcgggccacg
     1081 agccctgctc ccaccatgac gtcggggttc gatgtgttgg tgcacgatgt gatggctgcg
     1141 atgacaacgt cgccgtcgga gagcttgaac tcgtcgccgt catacacgac gtcgaccccc
     1201 accttggcct tgagcacccc ggtcggggcg ggactgccac cctcgtcctc ccagctgacc
     1261 accgtcgatg tcttcgcccc cccgttcttg ccgaagagat tggtcaggtc cgacttccac
     1321 tgcggctcca tcgtggacag cgcgattcga tcctggggtc gtctcgggcc ggccagcgat
     1381 ggctccactg tcgacatgtc gagctcgagc tcggcggagt aggtgatcgc ccggctgtcg
     1441 tcacgccaca tgccctggag cttgtagtac tgctccacgg catcgatcag cttgtcatcg
     1501 cgaccggtca ggcggaggta cttgagcgtc tgctcgtcga ccgggaagaa gccgacggtg
     1561 gcaccgtatt ccggcgccat gttggcgatg gtcgcccggt tagccaccgg catgtcggcg
     1621 agaccaggac cgtggaactc gacgaacttg tcgacgaccc catgctccct gagaatctcg
     1681 gtgacccgga gcaccaggtc cgttgccgtc gcgccttccg gcagtctctc ggtgagcctg
     1741 aacccgacga cctcgggaag gagcatgtgg atcggctggc cgaccatcgc cgcctcggcc
     1801 tcgatcccac cgacccccca gcccacgacg ccgagcccgt tgatcatggt ggtgtggctg
     1861 tcggtcccga ccaggctgtc gggatagagc cactgcccgt cgtcccaggc caccttggcc
     1921 aggtactcga gattgacctg gtggacgatc ccggtggccg gggggaccac tctgaagttg
     1981 gcgaagccgg actgtcccca tttcaggaac tcgtaacgtt cctcgttgcg ctcgaactcc
     2041 ttcctggagt tgatcaccag ggcgtcggag cgggcgaagg cgtccacctg gaccgagtgg
     2101 tcgacgacca ggtccgcagg gatcagaggg ttgacccgct gcgcggcccc ggggtccccg
     2161 gtgagccgga cgatcgcgga gcgcatcgct gccagatcga ccacggcagg gacgccggtg
     2221 aagtcctgga ggacgacacg cccggggacg aaggggatct cctgctcgcc gacgttggtt
     2281 gcgtcgtagg cggcgatgcg ccggacgtcg ttctcgtgca ccgagagccc gtcgaggttg
     2341 cgcagggccg actcgagcag gaccttgatg gagtagggga gacgctccac cccctcgatc
     2401 gcgtcgagtc ggacgatgtc tcggctgccg agcgcagtgt ccagggtggc ccgggaccca
     2461 aagggatcaa tgaacatgga gacctttcac ctgatcgaga accaagccaa ggctactgat
     2521 gacccgaagt agcgcagcag tggaggcgtc cgcaccgctg aggggtttgc ggtttccgaa
     2581 agaactggcg gttttccccc gaatccccgc tgaccgtcta tccgatcgtc accgggaccc
     2641 gagtgacaca ggcctcctcc agcacggtgt gggggatgat caccgggtca caggcatagg
     2701 agagggcacc gaacatcccg gcaacgatgc cgcgatcgag cgagcagatg acctcgggat
     2761 gactggttgc ggcttcgccg aacgggcaat aggaggtgag gagtctctgg cccccgatgt
     2821 cggggtcgac cgagaacccg agaccggtca ttgcgctggc tactgcctgc acggcgccgt
     2881 catagccagg ctgttcgggg acgccgatct ccgcggcgat ctcctgtccg taggccctgc
     2941 ccaccgactc ggcgacctcg gacaggttct cgggcccgac ccggtcgagg atcctgatca
     3001 gcatctccac cagcatctcg aagcggcgtg gagcgaagtg gaccgagact tccttggctg
     3061 acgcctcgta gcacttggcg ggtcggcctg ctcccggccc acccttgccc gaaggtcgct
//
LOCUS       DATITW010000797         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_239303, whole genome shotgun sequence.
ACCESSION   DATITW010000797 DATITW010000000
VERSION     DATITW010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239303"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VLS86_11135"
     CDS             <1..1174
                     /locus_tag="VLS86_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HSO51092.1"
                     /translation="ITIDGTSGLVFLGEVDLVEPESIPELQELLTWADGIRRLEVRAN
                     ADTADDATLARGRGAAGIGLARTEHMFLGERLAIVQRVILSNNPEERVQALSDLEQQQ
                     IGDFETILEAMDGLPVVIRLLDPPLHEFLPSRLELEHEMLGRVRAGRPIDDLQAMSDQ
                     VARWEEDNPMLGLRGVRLGLMLKDLYRMQARAATTALTRRLQAGGDPRLEIMIPLVAA
                     AEELIRMREIIKEELARATAGSGVELEIPIGTMIELPRAALTAGEIAKVADFFSFGTN
                     DLTQMTYGLSRDDAEGLFLRDYLEQGILSSDPFQTIDRDGVGRLIRVATKEGREANPA
                     LTVGLCGEHGGDPASIAFVHDLGLDYVSCSPPRIEGARLAAAQAALGDKDSAHRVD"
     gene            complement(1154..1864)
                     /gene="meaB"
                     /locus_tag="VLS86_11140"
     CDS             complement(1154..1864)
                     /gene="meaB"
                     /locus_tag="VLS86_11140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HSO51093.1"
                     /translation="MSDSVTARRVRDGDRRALARLITNIESGDPHAQAVVDELIASGT
                     IARRVGITGPPGAGKSTLVNSMVGELRSSGETVAVLAVDSSSPITGGALLGDRIRMQD
                     HVDDPEVYVRSMASRGHLGGLAIAASPTIATLAHAGFDVVLVETVGVGQSEIEVMDLE
                     DVVVLVLGPGWGDSIQADKAGILEISDVFVVNKADRPGVDIVQRALLDSLGDSEAPVL
                     LTTATTGEGVPALVDAVR"
     gene            complement(1903..2202)
                     /locus_tag="VLS86_11145"
     CDS             complement(1903..2202)
                     /locus_tag="VLS86_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HSO51094.1"
                     /translation="MRAEGPIDHDKLRETLDYIRPAVQADGGDLILLGTDGGTVNIQM
                     VGACGGCPLSMMTLKAGIERILIDRVPGVDEVNASADAPGLDDSEAINGIQLPVL"
     gene            complement(2298..>2726)
                     /locus_tag="VLS86_11150"
     CDS             complement(2298..>2726)
                     /locus_tag="VLS86_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51095.1"
                     /translation="PQYTIRSEMRIGRFHNEVYPNPHYVVEGGGWGHLIGMSQFGAQA
                     MAESGATYPEILAHYYGGLTPVPGESFLPDELLVGLMVGAETVSVSSDALLRADIDGV
                     VHEAPAPGVWEFDLEDGRIRVKVPRANPPLVPPGPPLTPF"
BASE COUNT          475 a          925 c          879 g          447 t
ORIGIN      
        1 gatcaccatc gacggcacgt ccgggttggt ctttctcggg gaggtcgacc tcgtcgagcc
       61 ggaatcgatc cccgagctgc aggagctcct cacctgggcc gacgggatcc gcaggctcga
      121 ggtgcgggcc aacgccgaca ccgccgacga cgcgacgctg gccagaggcc gcggcgcggc
      181 agggatcggc ctggctcgaa ccgagcacat gttcctcggt gagcgactcg ccatcgtgca
      241 gcgggtcatt ctctccaaca accccgaaga gcgggtgcaa gccctcagcg acctcgagca
      301 gcagcagatc ggtgacttcg aaacgattct ggaggcaatg gacgggctgc cggttgtgat
      361 ccggctcctc gacccccccc ttcacgagtt tctcccatca cgactcgagc tcgagcacga
      421 gatgctgggg cgggtgcggg ccgggcgccc gatcgacgac ctgcaggcaa tgtccgacca
      481 ggtggcgcgt tgggaggagg acaacccgat gctcgggctg cgtggggtcc gtctcgggct
      541 gatgctcaag gacctgtacc ggatgcaggc ccgagcggca accaccgcgc tcacccggcg
      601 tctgcaggct ggcggcgacc cccgtctcga gatcatgatc cccctggtgg ccgcggccga
      661 agagctgatc cggatgcgcg agatcatcaa ggaggagctc gccagggcga cggcaggctc
      721 gggcgtcgag ctcgagatcc cgatcgggac catgatcgag ctccccaggg ccgccctgac
      781 cgcgggtgag atcgccaagg tggccgactt cttctccttc gggaccaacg acctgaccca
      841 gatgacctac gggctgagcc gtgacgacgc cgaaggcctg ttcctgcggg actacctgga
      901 acagggaatc ctgtcgtctg acccgttcca gaccatcgac cgtgacggtg tgggtcgtct
      961 gatccgggtg gcgaccaagg aaggccggga ggccaacccc gcgctcaccg tcgggctgtg
     1021 tggagagcat ggcggcgacc cggcctccat agccttcgtc catgatctgg ggctcgatta
     1081 tgtgtcctgc agcccgcccc ggatcgaagg agcacgtctc gccgcggccc aggccgcgtt
     1141 gggcgacaag gactcagcgc accgcgtcga ctagcgccgg caccccttct ccggtggtcg
     1201 cagtggtgag gaggaccggt gcctccgagt cgccgagact gtccagcagc gcccgctgga
     1261 cgatgtcgac tcccggccgg tcagccttgt tgacgacaaa gacgtccgag atctcgagga
     1321 tcccggcctt gtccgcctgg atcgagtccc cccagcccgg gccgaggacc aggaccacga
     1381 catcctccaa atccatgact tcgatctcgg attgcccgac tccaacggtc tccaccagga
     1441 cgacgtcgaa cccggcatgg gcgagagtcg cgatggtcgg ggaggcggcg atggccaggc
     1501 cgccgagatg gccccgcgaa gccatcgagc gcacgtagac ctcagggtcg tcgacatggt
     1561 cctgcatccg gatccggtcc ccgagcagcg ccccaccggt gatcgggctc gacgagtcga
     1621 ccgccagaac ggccaccgtc tcaccgctag atcgcagctc accgaccatc gaattgacca
     1681 gggtcgactt gccggcgccg ggtgggccgg tgattccgac tctgcgggcg atggtgcccg
     1741 acgcgatcaa ctcgtcgacg acggcttggg cgtgagggtc acccgactcg atgttcgtta
     1801 tcagcctggc gagagcgcgg cggtcgccat ctcgcacccg gcgagccgtc acagagtcgc
     1861 tcattccgcg acagattacg gagtcgatcg ctcgccgagc gatcagagaa cgggaagctg
     1921 gatcccgttg atggcctcag agtcgtcgag gccgggggcg tcggcgctcg cattgacctc
     1981 gtcgaccccg gggacgcgat cgatcaggat tcgctcgatt cctgccttga gcgtcatcat
     2041 cgagagcggg caaccaccac aggcaccaac catctgaatg ttgaccgtcc cgccatctgt
     2101 gccgagcagg atcaagtcac ccccgtccgc ctgaaccgca ggccggatgt aatcgagggt
     2161 ctctctgagc ttgtcgtgat cgatcgggcc ttcagccctc acgctgctcg aaatggtcac
     2221 ttgtgtctct ggcatcgcgc ttgacaaccc tcggatcgtg cagatttgtt cccattctac
     2281 tgtgcgcccg gctccggtca gaacggtgtc aacggtgggc cgggtggcac caacggtgga
     2341 ttggcccggg gcactttgac ccgaatccgg ccgtcctcca agtcgaactc ccaaacgccg
     2401 ggcgccggag cctcatggac cacgccatcg atgtcggccc ggagcaacgc gtccgagctc
     2461 acgctcaccg tttcggctcc caccatcaac cccactaaca gctcatcggg aaggaaactc
     2521 tcccccggaa ccggggtcag accgccgtaa tagtgggcga ggatctcggg ataggtggcc
     2581 ccgctctcgg ccatcgcctg ggcgccgaac tgcgacatcc cgatcagatg cccccacccc
     2641 ccgccttcga ccacatagtg ggggttgggg tagacctcgt tgtgaaagcg cccgatgcgc
     2701 atctcgctgc ggatggtgta ctgcgg
//
LOCUS       DATITW010000798         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_319429, whole genome shotgun sequence.
ACCESSION   DATITW010000798 DATITW010000000
VERSION     DATITW010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319429"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VLS86_11155"
     CDS             complement(<1..221)
                     /locus_tag="VLS86_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO51096.1"
                     /translation="MTAGYQGGDVVRNVDFHVDAGEAVMIIGSNGAGKTTLFRAVLGL
                     LPPSSGSVEFQGESITGLPTERVARLGIS"
     gene            complement(232..975)
                     /locus_tag="VLS86_11160"
     CDS             complement(232..975)
                     /locus_tag="VLS86_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSO51097.1"
                     /translation="MPYLELNEIGKHLGGLPAVVGVSVGADVGEILAIVGPNGAGKST
                     LLKLIVGLEEPTSGTVRLGEHDITGFPTHRVRRLGVAMVQQTPRAFATMTVLENATLG
                     AMFGTGEDHRGESEAIEIGAEMLALVGLKDRWDDHVGNLNLHEPRFQELARALSGYPK
                     LLLLDEVMAGLNDAELSASIEMVRTIRDRLGITVVWVEHVMQAVMQLAERVVVLNFGR
                     VLAEGPAEVVMRDESVVDAYLGSGGIDRA"
     gene            complement(983..2284)
                     /locus_tag="VLS86_11165"
     CDS             complement(983..2284)
                     /locus_tag="VLS86_11165"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSO51098.1"
                     /translation="MNRGLPRSRLLVSIVGLALVVAACGDGAGDSTTTAAPGTTEATA
                     TTEPTATTEPTGTPAPASGEPIKIGILTDLTGFTPWAVNARDGMLLAAEEINAAGGVD
                     GRMIEIVQEDSANDADAGTSGFERLAEAEVVAIGGIISSTVGGTVAPLAEELQIPTFT
                     VKSGTEAALTQDSRFLFRSCLPAAPMTAPPVLQYAQEEGITNVGVIVADYPWGQSFKA
                     AVESTFEGSGIDFGEIQVAPVPPDTDFTPYVRALADAGVGMVIATGHPPGNAAIVTLS
                     ADLIGDVPVTGAWIPPDLAVGGLADLAIGRYFDFSCADYTSDGYAALAARYLAFSDNA
                     FMSDDAVAGFGIVTMVADAVANVGDDGVAISDYLHDQTYELEGYAHPVSWTEWGELAS
                     SAPVLHVISEGPAPAGLNEAGEWWLERLSQSETLEPYVPAS"
BASE COUNT          424 a          834 c          785 g          417 t
ORIGIN      
        1 aagctgatcc cgaggcgggc gacacgctcc gtgggcagcc cggtgatgct ctctccctgg
       61 aactccacgc tccccgagga gggcgggagc aggccgagca cggcgcgaaa cagcgtggtc
      121 ttaccggcgc cgttggagcc gatgatcatg actgcctcgc cggcgtcgac atggaagtcg
      181 acgttcctga ccacgtcgcc accctgataa ccggcggtca tgccttggac ctcaagcacg
      241 gtcgatgccc cccgagccga ggtacgcgtc caccaccgac tcgtctcgca tcacgacctc
      301 ggccgggccc tcggccagaa cccgcccgaa gttgaggacc accacccgct cggcgagctg
      361 catcaccgcc tgcatcacat gctcgaccca tacgaccgtg atcccgaggc ggtcacggat
      421 ggtccgcacc atctcgatcg atgcactcaa ctctgcatcg ttgagcccgg ccatgacctc
      481 gtcgagcagg agcagcttcg ggtacccgga caacgcccgg gccagttcct ggaaccgtgg
      541 ctcgtgcaga ttcaggttgc caacgtgatc gtcccaccgg tccttcagac cgaccagcgc
      601 gagcatttct gcgccgatct cgatggcttc ggattcgcct cgatgatcct ctccggtgcc
      661 gaacatcgca ccaagggtgg cgttctcgag gaccgtcatg gtggcaaatg cacgcggggt
      721 ctgctgcacc atcgccacac cgagtctgcg gacgcgatga gtgggaaaac cggtgatgtc
      781 atgctcgccc aggcgtaccg tcccactcgt cggctcttcg aggccaacga tcagcttgag
      841 cagggtgctc ttgccggcgc cgttggggcc gacgattgcg aggatctcac caacgtcggc
      901 cccgacgctc actccaacga cggccggcag accgcccaaa tgcttcccga tctcgttgag
      961 ctcgagatac ggcacctgaa cgtcaggacg ccggcacgta cggctccagc gtttcggact
     1021 ggctcagccg ctcgagccac cactccccgg cctcgttcag ccccgcgggg gccggacctt
     1081 cgctgatgac gtggagaacc ggagccgatg acgccagttc gccccattct gtccagctca
     1141 ccgggtgggc gtaaccctcc agctcatagg tctggtcgtg gaggtagtcc gagatcgcca
     1201 ccccgtcgtc gccgacattg gcaactgcgt cagccaccat cgtcacgatc ccgaagccgg
     1261 ccacggcgtc gtcagacatg aacgcgttat ccgagaacgc cagatagcgc gcggccaggg
     1321 cggcgtagcc gtcgctggtg tagtcggcgc aggagaagtc gaaatagcgg ccgatggcca
     1381 ggtcggccag ccctcccact gccaggtctg gcgggatcca tgccccggtg accgggacgt
     1441 cgccgatcag gtctgccgac aacgtgacga tggcggcgtt ccctggcggg tggcctgtgg
     1501 cgatcaccat tccgacgccg gcgtcggcca acgcccggac gtaaggggtg aaatcggtgt
     1561 ccggcggaac cggagcgacc tgaatctctc caaaatcgat cccggagccc tcgaaggtgc
     1621 tctcgacggc cgccttgaag gactgccccc agggatagtc ggcgacgatc acgccgacat
     1681 tggtgatgcc ttcctcctgg gcgtactgaa gcaccggtgg cgctgtcatc ggagccgccg
     1741 gcagacagct acggaacaag aaccggctgt cctgggtgag ggcggcctcg gtgcccgact
     1801 tgaccgtgaa ggtgggtatc tgcagttcct cagcgagggg agccacggtg ccgcccacag
     1861 tgctggagat gatgccaccg atggccacca cctccgcctc cgccagacgc tcgaatccgg
     1921 aagtgccggc gtcggcgtcg ttggccgagt cctcttggac gatctcgatc atccggccgt
     1981 cgacaccgcc ggcggcgttg atctcctcgg cggcgagcag catcccgtcc cgggcgttga
     2041 ccgcccacgg ggtgaacccg gtgaggtcgg tcaggatccc gatcttgatc ggctctccac
     2101 tggccggggc cggggtcccg gtgggctcgg tggttgcagt gggctcggtg gttgcggtgg
     2161 cctcggtagt gccgggagcg gcagtggtgg tggagtcacc agcaccatca ccacaagctg
     2221 ccaccaccag cgccagccca acgatcgaga cgagcagcct cgaccgggga agtcctcgat
     2281 tcatctcagt gtcctcctcg tcgtgcgctt agcgaacgtc cgtgcggcag acgaactcat
     2341 ccgagtgtaa caaacggcct tgtggcgtga cggtgtgcag actcaccact cattccatgc
     2401 gttgagcaca gtgttggtga acggttccgg ctggtccaga ttggccaggt gggcggcgcc
//
LOCUS       DATITW010000799         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_319431, whole genome shotgun sequence.
ACCESSION   DATITW010000799 DATITW010000000
VERSION     DATITW010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319431"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1335)
                     /gene="icd"
                     /locus_tag="VLS86_11170"
     CDS             complement(133..1335)
                     /gene="icd"
                     /locus_tag="VLS86_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HSO51099.1"
                     /translation="MATPITMGPNGLSVPDDPIVPFIEGDGTGPDIWAASVRVFDAAV
                     EKAYEGSRKLNWMEVLAGEKAHNAVGSWLPDETVEAFATYLVGIKGPLTTPIGGGFRS
                     LNVALRQILDLYACVRPIRWFEGVPSPVKEPGLVDMIIFRENIEDVYAGLELEEGSED
                     ASKLIAFLKAEYGWDVRSDSGVGIKPISITGSKRLIRAAINHALANDRKSVTLVHKGN
                     IMKFTEGAFRSWGYDLVREEFSDVAVSWEDCGGKPGDKLLVQDVIADAMLQQVLTRPG
                     NYDMIATTNLNGDYISDALAAQVGGIGIAPGGNINYDTGVAVFEATHGTAPKYAGLDK
                     VNPGSVILSGEMMFRYMRWFEAADSIIKGLEGAIRAKTVTYDFARLTDDATEVKTSEF
                     GDAIIGHM"
     gene            complement(1402..2247)
                     /gene="folD"
                     /locus_tag="VLS86_11175"
     CDS             complement(1402..2247)
                     /gene="folD"
                     /locus_tag="VLS86_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HSO51100.1"
                     /translation="MSALILDGKAVAAAVKAEVAEGVAALDYSPGLATVLVGDDPASH
                     TYVRGKRSDAGEVGIVSLHHELGADVSQGELEALIDALNRDDTVDGILVQLPLPGDLD
                     SERIVERISPAKDVDGLHPHNLGLLVLDRPGLRPCTPSGIMRILAHYEIPVSGARAIV
                     IGRSFLVGRPLALMLANRGVDATVSIAHSRTKDLAAVARAADILVAAVGSPEMVTADF
                     VKAGATVIDVGINRTERGMVGDVEFESVQEVAGAITPVPGGVGPMTRAMLLVNTLTAA
                     GGRRG"
     gene            complement(2249..>2648)
                     /gene="purH"
                     /locus_tag="VLS86_11180"
     CDS             complement(2249..>2648)
                     /gene="purH"
                     /locus_tag="VLS86_11180"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627741.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HSO51101.1"
                     /translation="GQETDAWETVSERAPTEGEKLDLGFAWAVAMNTKSNAIVIVKDG
                     AAVGVGAGDQSRVGAAERAVIKAGERARGGAAASDAFFPFRDGLDALAETGVTAVIEP
                     GGSRNDQEVIDAANEHGIALVFASNRHFRH"
BASE COUNT          475 a          887 c          815 g          471 t
ORIGIN      
        1 tcctcttcag gcaggacatc agaggaacgg gtgatctgga gggtggtcat gggctcccgc
       61 taggtgaaat cagtgtagga gaggcccgtg tgcagggagc caccggcaga gtggccgaag
      121 cacacgacac cgctacatgt ggccgatgat ggcgtcgccg aattcggaag tcttgacctc
      181 ggtggcatcg tcggtgaggc gggcgaagtc gtaggtcacg gtcttggccc tgatcgcacc
      241 ctccaggccc ttgatgatgg aatccgcggc ctcgaaccat ctcatgtagc ggaacatcat
      301 ctccccggag aggatcaccg atcccgggtt caccttgtcc agaccggcgt acttgggcgc
      361 ggtgccatga gtcgcctcga acaccgctac cccggtgtcg tagttgatgt tgccaccggg
      421 agcgatcccg atcccaccca cctgggcggc cagcgcgtcc gatatgtagt caccgttgag
      481 gttggtggtg gcgatcatgt cgtagttgcc gggtcgggtc aggacctgtt gcagcatggc
      541 gtcggcgatg acgtcctgga ccaggagctt gtcgcccggc ttccccccgc agtcctccca
      601 cgacaccgcc acgtctgaga actcctcacg caccaggtcg tagccccagc tgcgaaaggc
      661 gccctcggtg aacttcatga tgttgccctt gtggaccagc gtcaccgact tgcgatcgtt
      721 ggcgagggca tggttgatcg cggcgcgaat caaacgcttg gagccggtga tcgagatcgg
      781 cttgatgcca acccctgaat ccgaccggac gtcccaccca tactccgcct tgagaaaggc
      841 gatcagcttc gacgcatcct cgctgccctc ctccaattcg agaccggcgt agacgtcctc
      901 gatgttctct ctgaagatga tcatgtcaac gagaccgggc tccttgaccg gcgacggaac
      961 gccttcgaac catcggatcg gccgcacaca tgcgtaaaga tcgaggatct ggcgaagggc
     1021 gacgttgagc gagcggaagc cacctccgat tggagtggtg agcgggcctt ttatacccac
     1081 cagataggtg gcgaaagcct ccaccgtctc gtcgggaagc caggagccca ccgcgttgtg
     1141 cgccttctct cccgccagaa cctccatcca gttcagcttc ctggagccct cgtaggcctt
     1201 ctccacggcg gcatcgaaga cccgaaccga ggcagcccag atgtcggggc cggtgccgtc
     1261 accctcgatg aaggggacga tcggatcgtc gggaacactg agaccgttcg ggcccatggt
     1321 gatgggtgtg gccatgacag agagctcctg gaagaaacgg ggaccgggcc agggacacta
     1381 gccgggccgg ttatcgctcc gtcagccgcg ccggccgccg gcggcggtga gggtgttgac
     1441 caagagcatc gccctggtca tcgggcccac ccctccgggc acgggggtga tcgctcctgc
     1501 cacctcctga actgattcga actcgacatc gccgaccatt cctctttcag tccggttgat
     1561 gccgacgtcg atcacggtcg ctcccgcctt cacgaagtcc gcggtgacca tctcagggga
     1621 gccaaccgcc gcgaccagga tgtcggctgc gcgggcaacc gcggccaggt ccttggtgcg
     1681 ggaatgagcg atcgaaacgg tggcgtccac tccacggttg gcgagcatca gtgccaatgg
     1741 ccgacccacc aggaacgagc ggccgatgac aatcgcccgg gcccctgaaa ccgggatctc
     1801 ataatgggcc agaatccgca tgatcccgct cggggtgcat ggcctcagac ctggccggtc
     1861 gagcacgagg aggccgaggt tgtgcggatg caacccgtcg acgtccttcg ccggggagat
     1921 tcgctcaacg atccgctccg agtcgagatc acccggcagt ggcagctgaa caaggatccc
     1981 gtccaccgtg tcgtcgcggt tgagagcgtc gatcagcgcc tccaattctc cctgagagac
     2041 gtctgcgccc aactcgtggt gaagggacac gatgccgacc tccccggcat cgcttcgctt
     2101 gcctctcaca taggtgtgcg aagcgggatc gtcacccacc agcacggtgg ccagacccgg
     2161 cgagtagtcg agagcagcca caccctcggc cacctcggcc ttcaccgcgg ctgcgaccgc
     2221 ctttccgtcg aggatcaatg cgctcatatc aatgccgaaa atgccggttg ctggcgaaga
     2281 cgagggcgat cccgtgctcg ttggcggcgt cgatgacttc ctggtcgttg cgcgacccac
     2341 cgggctcgat gactgcggtc accccggtct cggccagcgc gtcgagaccg tcgcggaacg
     2401 gaaagaaggc gtcagaggcc gccgccccac cccgcgcccg ttcacctgcc ttgatcaccg
     2461 ctcgctcggc cgctccaacc cgcgactggt ctcctgcccc caccccaacg gccgctccgt
     2521 ccttgacgat cacgatcgca ttcgatttgg tgttcatcgc caccgcccag gcgaatccca
     2581 gatcgagctt ctccccctcg gtcggagccc gctctgagac ggtctcccag gcgtcggtct
     2641 cctgcccg
//
LOCUS       DATITW010000800         1513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_759922, whole genome shotgun sequence.
ACCESSION   DATITW010000800 DATITW010000000
VERSION     DATITW010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759922"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1148)
                     /locus_tag="VLS86_11185"
     CDS             complement(69..1148)
                     /locus_tag="VLS86_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSO51102.1"
                     /translation="MTATLERKSTSTPALAGRPPKRRSQGWIITGITGLVLFVFLLPL
                     GFMVITSLKSEQMLSDFEGPILPQSAATFEYEGEQLDIYTVPMPDGTERELAMLQPGR
                     QTSQFVDPADPSAGPIEWEGNWRLLEPAYALDPQWQNYSQAWDLMDFPLLLRNTLVIA
                     GLGTIGAVGSSILVAYGLARFHVPFGRSIMVVLIATIILPRYATLVPTYTLFLRIGWV
                     GTWLPLIVPHFVANAFNVFLLRQYFLTIPRELDEAATIDGAGPFRTLWSVLLPQMKAP
                     IVAVSLFHFYFAWNDFFEPLIYLSSRPELQPIAVGLRTFNTLYDDTPSLIQSGAMLAL
                     IIPVLVFLFAQRVFLKGIDLSGVQK"
     gene            complement(1153..>1513)
                     /locus_tag="VLS86_11190"
     CDS             complement(1153..>1513)
                     /locus_tag="VLS86_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSO51103.1"
                     /translation="MKGVPSDLYAAARIDGAGEWRSFRHITIPFISPIIFYNLVITLI
                     GIFGYFLVPFVLNNGSGAPNGATQFYALYFFRTGFQFFDMGYAATLAWALFIVAVLTT
                     GLLFWSAKYWVHYEFEG"
BASE COUNT          332 a          426 c          504 g          251 t
ORIGIN      
        1 gaaaccgtgc gggaactccg atgcggcgaa catttaccct ccgaccttcc tcattctcgg
       61 tcacctacct atttctgcac gccggagagg tcgattccct tgaggaacac cctttgcgcg
      121 aagaggaaca ccagcaccgg gatgatcagg gcgagcatgg ctcccgactg gatgaggctg
      181 ggcgtgtcgt cgtagagcgt gttgaaagtg cgcaggccca ctgcgatcgg ctgcaattcg
      241 ggccgtgacg agaggtagat caggggctcg aaaaagtcat tccacgcgaa gtagaaatga
      301 aagagggata cggccacgat cggggctttc atctggggga ggaggaccga ccacagagtg
      361 cggaacggcc cagccccgtc gatggtggcc gcttcgtcga gctccctggg gatggtgagg
      421 aaatactgtc tgagcaggaa cacattgaag gcgtttgcga cgaagtgggg cacgatcaac
      481 ggcagccatg tgcccaccca accgatgcgg aggaagagtg tgtaggtggg aacaagtgtg
      541 gcataacgag ggaggatgat cgtcgcgatc agcaccacca tgatcgacct gccgaacggc
      601 acgtggaagc gggccaaccc gtaggcgacc aggatggagg acccgaccgc cccgatcgtc
      661 cccagtccgg cgatgaccag cgtgttcctc agcagaagcg ggaagtccat caagtcccag
      721 gcctggctgt agttctgcca ttgcgggtcg agcgcgtagg cgggctcgag caacctccag
      781 ttgccttccc actcgatagg gccggcggac gggtcggccg ggtcgacgaa ctgagatgtc
      841 tgccgccccg gctggagcat cgccagctct cgttccgtgc catcgggcat gggaacggtg
      901 tagatgtcga gttgctcgcc ctcgtactcg aaggtggctg ccgactgggg aaggatcgga
      961 ccctcgaagt cggagagcat ctgctcggac ttgagcgagg tgatcaccat gaatccgagg
     1021 ggaagaagga acacgaacag gaccaacccg gtgatcccgg tgataatcca gccctgagac
     1081 cgcctcttcg gaggacgacc ggcaagggcg ggggtcgagg tgctcttgcg ctcgagggtg
     1141 gcggtcatcg tctcatccct cgaactcgta gtggacccag tacttggccg accagaacag
     1201 gagccccgtc gtcagcaccg cgacgatgaa cagagcccag gccaatgtcg ccgcgtatcc
     1261 catgtcgaag aactggaacc cggtcctgaa gaagtagagg gcatagaact gggtagcccc
     1321 gttgggtgcg ccgctgccgt tgttgaggac gaaggggacc agaaagtagc cgaagatccc
     1381 gatcagagtg atcacgaggt tgtagaagat gatcggggag atgaagggga tggtgatgtg
     1441 ccggaacgac cgccactccc cggcgccatc gatgcgcgct gccgcataca gatcagacgg
     1501 cacccccttc atc
//
LOCUS       DATITW010000801         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_679344, whole genome shotgun sequence.
ACCESSION   DATITW010000801 DATITW010000000
VERSION     DATITW010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679344"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(369..1184)
                     /locus_tag="VLS86_11195"
     CDS             complement(369..1184)
                     /locus_tag="VLS86_11195"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HSO51104.1"
                     /translation="MGLTLGVAALMVALQLGSSDFGDPGSMGTTPTASVPSSGPPPTA
                     PATTTSTGPEPAWQPNPRSVLGDTEPGLPAPIRLRIPTLGVDAPIEGYGVNRGTGQMD
                     IPDNATDVAWYRHGPAPGEPGSAVLAAHVDVSGQGPGVFFDLDDLEAGDEIVVSYEDR
                     SERRFVVSARQTFLKTELPVEDIFDREGPPLLTLVTCGGGFSASTRSYDSNVVVYSVP
                     MESTGARQPGTASGQSDEPRARMFPSESLNHAVFSPSGDTRTPSTVRKSSPKS"
     gene            complement(1299..>1500)
                     /locus_tag="VLS86_11200"
     CDS             complement(1299..>1500)
                     /locus_tag="VLS86_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51105.1"
                     /translation="VGSVEGGSFQLVTAAITGLGSSPEGVPTGSGGLATGDNSLLLPI
                     ALAGLALAGVAAGSVALARREG"
BASE COUNT          267 a          502 c          499 g          232 t
ORIGIN      
        1 gccctccccg ggatcctcca cgctattgcg gcggggcggc ccgtgcgccg ccaagcaaga
       61 aggcgacctc aaccagggag acagcgatct aggttgcagt gggaaggaga ggcttccatg
      121 gacttcatac tcaagctcga acttccggcg tcggacgtcg atcgggccag ggcctggtat
      181 tcggacaagc tggggctgga accgatcgtc tccggcgagg agctgaccta tcaggccggt
      241 gccacccgat tcgggatcta tccctctgcc aacgccggca ccaaccgggc cacggcggcc
      301 aggctcatcg tcgccgactt cgatgcggcc cgggccgatc tgctcgcgaa gggcgtcgtc
      361 ttcgaggact acgacttcgg tgacgacttc cgcaccgtcg acggggttct ggtgtcaccc
      421 gacggggaga agacggcatg gttcaaggac tccgaaggga acatcctcgc cctcggctcg
      481 tccgactgac ccgacgccgt cccgggctgc cttgcgccgg tcgactccat cggcaccgag
      541 tacaccacca cgttgctgtc ataggaccgg gtcgacgcgc tgaagcctcc cccgcaagtg
      601 accaaggtga gcagtggagg gccttcacga tcgaagatgt cctccaccgg cagctccgtc
      661 ttgagaaagg tctgccgggc cgataccacg aagcgtcgct ccgagcggtc ctcataggaa
      721 acaacgatct cgtcacctgc ttcgagatcg tcgagatcga agaagacacc agggccctga
      781 ccgctgacgt cgacgtgggc tgccagcacc gccgaccctg gctcgccggg cgctggccca
      841 tgtcgatacc aggcgacatc ggtggcgttg tcggggatgt ccatctggcc ggtcccgcgg
      901 ttcactccgt agccctcgat cggggcatcg actcccaagg tcggaatccg gagacggatg
      961 ggggcgggga gacctggctc ggtatctccc aaaaccgagc gcgggtttgg ctgccaggcc
     1021 ggctccggcc cggtgctggt cgtcgtggca ggagcggtcg gcggtgggcc ggagctcggc
     1081 accgaggcgg tcggcgtcgt tcccatcgag cccgggtctc caaaatcgga gctgcccaac
     1141 tgcaaagcca ccatcaatgc ggcgacgccg agggtcaggc ccacggcgat caatgcccat
     1201 cctcgtgtcc gatgctcggc caactgactc tccaggtgca atgagtgagg gagacctgcc
     1261 gggggcaggt ctccctcata cggtagaact cgcgccgttc agccctcgcg gcgagcgaga
     1321 gcgaccgagc cagcggcaac gccggccagg gccaggccgg ccagggcaat cggaagcaac
     1381 aggctgttgt cgccggttgc gagcccgccg ctgccggtgg gaacgccctc aggggaggat
     1441 ccaagaccgg tgatggccgc agtcaccagc tgaaagctgc cgccttcgac cgagcccacg
//
LOCUS       DATITW010000802         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_679370, whole genome shotgun sequence.
ACCESSION   DATITW010000802 DATITW010000000
VERSION     DATITW010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679370"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /gene="uppS"
                     /locus_tag="VLS86_11205"
     CDS             <1..581
                     /gene="uppS"
                     /locus_tag="VLS86_11205"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HSO51106.1"
                     /translation="WLTAFTFSTENWSRSPDEVEFLMWFNEDLLTRRRDDLDAQGVRM
                     LFAGDMGDPRIPDRNRRHMAEAAELTAANTKLNLVFAFNYGSRREIVEAARSLASQVL
                     NSEIAIVDIDEASIARALYIPDMPDVDLVIRSSGEHRLSNFLLWQSAYAEFYFPGILW
                     PDFDRHGLLDAVIDYQGRERRFGAAEDAPQID"
     gene            615..1922
                     /locus_tag="VLS86_11210"
     CDS             615..1922
                     /locus_tag="VLS86_11210"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HSO51107.1"
                     /translation="MTEPTLDTISNLAKKRGFVFQSSEIYGGIRSAYDYGPLGVELLR
                     NVKEQWWRDMVQTRDDVVGIDSAILQARQVWQASGHEEVFTDPLVECRNCNARHRVDK
                     LEDPNTCPTCGKSGTFTEPRNFNLMFRTHMGPVDSEDNLIYLRPETAQGIFTNFENIR
                     RTNRLKLPFGIAQVGKSFRNEITPGQFVFRTREFEQMEMEYFVRPEEADQWFEYWKEK
                     RFKWYTDLGMTEANLRIRAHEADELSHYSAGTVDIEYRFPWDWDELEGVANRTDFDLK
                     QHSEHSGEDLRYFDPDSDERFFPYVIEPAAGATRTTFAFLIDAYHEEKVNDEERTVLR
                     LHHRLAPYKVAVLPLSKKPELIEPATRIATELRQRWMVEMDVTQSIGRRYRRQDEIGT
                     PYAVTVDFDSLDDHAVTVRDRDTMLQERVSMDRLADLLSAKLS"
     gene            1965..2684
                     /locus_tag="VLS86_11215"
     CDS             1965..2684
                     /locus_tag="VLS86_11215"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HSO51108.1"
                     /translation="MRRAILWVLASVLVIGGGAVAYLYLAGGSGEPSTELTTPSLATG
                     TTTTEDSAPDTTPPEAPSAFVIDAAQSTATFEIDEVLRGSPQRVIGTTSELAGQIQVD
                     PSDLATVEFSPIVVNARTFQTGSGNRDRAIRGPVILNSASDEFELITFDVTTVDGLSG
                     SLAIGEPVSFTVGGDLLIRDTTTNVTFEVSATLVDESTIEGSAESTVLRSDYGIGIPS
                     APGVADVGDEVVIRLDFVATS"
BASE COUNT          550 a          957 c          882 g          492 t
ORIGIN      
        1 aatggctcac cgcgttcacc ttctccaccg agaactggtc gcgatccccg gacgaggtcg
       61 agttcctgat gtggttcaac gaggacctac tgacccgacg cagggacgac ctcgacgccc
      121 agggggtcag gatgctgttc gccggcgaca tgggcgaccc ccgcatcccc gatcgcaatc
      181 gccggcacat ggccgaggcc gccgagctga ccgccgccaa caccaagctc aacctggtgt
      241 tcgccttcaa ctacggcagt cgccgggaga tcgtcgaagc ggcgagatcg cttgcctccc
      301 aagtgttgaa cagcgagatc gccatcgtcg acatcgacga ggcatcgatc gctagagcgc
      361 tctacatccc ggacatgccg gatgtggacc tggtgatacg gtcgtcggga gagcaccggc
      421 tctccaactt cctcctctgg caatcggcct atgccgagtt ctacttcccc gggatcctct
      481 ggcccgactt cgaccgtcac gggctgctcg acgcggtgat cgactaccag gggcgggagc
      541 gacgatttgg cgcagccgag gacgctcccc agattgacta accgacccga ccgggccacc
      601 gttcccacct cgaaatgaca gaaccgaccc tcgacaccat ctccaacctg gccaagaagc
      661 gtggcttcgt cttccaatct tccgagatct acgggggaat ccgctccgcc tacgactacg
      721 gcccgctcgg cgtcgagctg ctccgcaacg tcaaggagca gtggtggcgg gacatggtgc
      781 agacccggga cgacgtggtc gggatcgact cggcgatcct ccaggcgcgt caggtgtggc
      841 aggcgtcggg ccacgaggag gtgttcaccg atcccctggt cgaatgtcgc aactgcaacg
      901 cccgtcatcg tgtcgacaag ctcgaggatc caaacacttg ccccacgtgt ggcaagtcgg
      961 ggacgttcac cgagccccgc aacttcaacc tcatgttccg cacccacatg ggaccggttg
     1021 actccgagga caacctgatc tatctccgcc cggagaccgc ccagggcatc ttcaccaact
     1081 tcgagaacat ccgccgcacc aatcggctca agctcccgtt cgggatcgcg caggtgggca
     1141 agtcgttccg aaacgagatc acccccggcc agttcgtgtt tcgcacccgg gagttcgagc
     1201 agatggagat ggagtacttc gttcgccccg aggaggccga ccagtggttc gagtactgga
     1261 aagagaagcg cttcaagtgg tacaccgatc tcgggatgac cgaggccaac ttgagaatcc
     1321 gcgcccacga ggccgatgag ctcagccact actcggcagg gaccgtggac atcgagtacc
     1381 gcttcccctg ggactgggac gagctggagg gagtggccaa ccgcaccgac ttcgacctga
     1441 aacagcactc cgagcactct ggggaggatc tccggtattt cgatcccgac agcgacgagc
     1501 gcttctttcc ctacgtgatc gagccggcag ccggggccac caggaccacc ttcgccttcc
     1561 tgatcgatgc ctatcacgag gagaaggtca acgacgagga gcggaccgtg ctccggcttc
     1621 accaccgcct cgctccgtac aaggtcgccg tgctgccgct ctccaagaag cccgagctga
     1681 tagagcccgc gacccggatc gcaaccgagc tgcgtcagcg ttggatggtg gagatggacg
     1741 tgacacagtc gatcggccgc cgctatcgac gtcaggacga gatcggcacc ccatatgcgg
     1801 tgaccgtgga cttcgactcg ctcgacgatc acgcggtgac ggtgcgggat cgagacacga
     1861 tgctgcaaga gcgggtttcg atggaccggc tggccgatct cctctcggcg aagctgtcct
     1921 gaggtttacc cgaccttgac acggtgaccg gagactgtga cagaatgcga cgagcgatcc
     1981 tctgggtgct ggcttcggtc ctggtgatcg ggggaggcgc cgtggcctac ctctatctgg
     2041 ccggcggctc aggtgagccc tccaccgagc tgaccacacc cagcctggca acgggcacga
     2101 ccaccaccga ggactccgct ccggacacca cgccaccgga ggcgccgtca gcctttgtga
     2161 tcgatgccgc ccaatccacc gccaccttcg agatcgacga ggtgctgcgc gggtcgccac
     2221 aacgggtcat cggcaccacc tccgagctcg ccgggcagat ccaggtcgac ccctcagacc
     2281 tcgccaccgt cgagttctcc ccgatcgtgg tcaatgccag gaccttccag accggctccg
     2341 ggaaccgtga ccgggcgatt cgggggccgg tgattctgaa ctccgcgagc gacgagttcg
     2401 agctcatcac cttcgacgtg accacggtcg acggactgag cggttctctg gccatcggcg
     2461 agccggtgtc gttcacggtg ggcggcgacc ttctcatccg ggacacgacc accaatgtga
     2521 cgttcgaggt gtctgcgacc ctggtggacg agagcaccat cgaaggctca gccgagagca
     2581 cagtgcttcg ctccgactac ggcatcggca tccccagtgc ccccggggtg gcggacgtcg
     2641 gggatgaggt cgtgatccgt ctcgattttg tcgctacctc gtgatcgaac ccctggtcgt
     2701 acttatgatc gaccggcgat ggcagaacga ggagacatag accgggtccg ggactcgacc
     2761 gacctcgtcg agttgatctc agaggtgacc aaggtcaagc gctcgggccg cacgttcatg
     2821 gcggtttgcc ccttccacga ggagaagacc ccgtcgatgt cggtggatca ggcacgcggg
     2881 c
//
LOCUS       DATITW010000803         1591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_39275, whole genome shotgun sequence.
ACCESSION   DATITW010000803 DATITW010000000
VERSION     DATITW010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39275"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VLS86_11220"
     CDS             <1..232
                     /locus_tag="VLS86_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683798.1"
                     /note="with PdxT forms pyridoxal 5'-phosphate from
                     glutamine, either ribose 5-phosphate or ribulose
                     5-phosphate, and either glyceraldehyde 3-phosphate or
                     dihydroxyacetone phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HSO51109.1"
                     /translation="SDAVLMMSLGVDGVFVGSGIFKSSHPEAYARAIVEATTFWTEPE
                     VVAKVSRGLGEAMPGIEIDTLKDSERLASRGW"
     gene            234..797
                     /gene="pdxT"
                     /locus_tag="VLS86_11225"
     CDS             234..797
                     /gene="pdxT"
                     /locus_tag="VLS86_11225"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HSO51110.1"
                     /translation="MTSVGVLALQGDVREHLRTLESIGVNAVEVRKPDQIGSIDAMVI
                     PGGESTTIGKMSARFGMLDPLRSAVAGGLPTFGTCAGLILLSGAVTDGDQPLLGVLDV
                     VVRRNAFGRQNESFEADLEVSGLGGPFHAVFIRAPWVEKVGAEVEVLAAIEEHPVMVR
                     QGNILASSFHPELAGDGRIHQMLIDLI"
     gene            809..1558
                     /locus_tag="VLS86_11230"
     CDS             809..1558
                     /locus_tag="VLS86_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSO51111.1"
                     /translation="MSGHSKWANIKHRKGRQDAARGKLFGKLAKAIEIAAREGGANPD
                     FNPVLATAIFKAKSASMPNDNIDRAVKRGSGEVEGAHYEEIWYEGYGPGGVALYVQIL
                     TDNRNRAASDVRSTFTRSGGNLGEPGSVGYLFSQKGLIEVKGSEDEVMLVALESGAED
                     IRDNGDGWLEVITSPGDLQAVRSSLAEAGIEIESADVTQLPSTTVPVGEGDARRLLRL
                     IDALDDLDDVQSVYSNYDIEDDVMERVLEEV"
BASE COUNT          276 a          475 c          552 g          288 t
ORIGIN      
        1 atccgacgct gtcttgatga tgtcgctcgg tgtcgacggc gtgttcgtcg gctccgggat
       61 cttcaagagc tctcatcccg aggcctatgc ccgtgccatc gtcgaggcga cgaccttctg
      121 gaccgagccc gaggtggtgg ccaaggtctc caggggtctg ggcgaggcga tgccgggcat
      181 cgagatcgac accctcaagg acagcgaacg gctcgcctcc cgggggtggt gagatgacct
      241 ccgtgggcgt ccttgccctg cagggggacg tcagggagca tctgcgcact ctcgaatcca
      301 tcggtgtcaa cgcggttgaa gtcaggaagc cggaccagat cgggtcgatc gacgccatgg
      361 tgatccccgg gggggagtcg accaccatcg ggaagatgtc tgcccggttc gggatgctcg
      421 acccgcttcg gtccgcggtg gccggcggtc tccccacttt cgggacctgc gccggattga
      481 tccttctctc cggcgcggtg acagatgggg atcagccgtt gctcggcgtc ctcgacgtgg
      541 tcgttcgacg caatgcgttc ggtcgtcaga acgagtcttt cgaggccgac ctggaggtct
      601 ccgggctcgg cgggccgttc catgcggtgt tcatccgggc gccatgggtg gagaaggtgg
      661 gagccgaggt ggaagtgctg gccgcgatcg aggagcatcc ggtgatggtg cgtcagggga
      721 acatcctggc ttcgtcgttt catcccgagc tcgccgggga tgggcgaatc caccaaatgc
      781 tgatcgatct gatatgaggt agctcgacgt gtccggtcat tccaaatggg ccaacatcaa
      841 gcatcgcaag ggacgtcagg acgcggccag gggcaagctg ttcggcaagc tggccaaggc
      901 catcgagatc gcggcccgcg agggtggggc caaccccgac ttcaacccgg tcctggcgac
      961 agcgatcttc aaggccaagt cggcttcgat gcccaacgac aacatcgatc gtgccgtcaa
     1021 gcgggggagt ggcgaggtcg aaggggccca ctacgaggag atctggtacg agggctacgg
     1081 cccgggtggt gtcgctctct acgtccagat cctgaccgac aatcgcaatc gagccgcctc
     1141 cgacgtcagg tcgactttca cccgaagcgg gggcaatctc ggcgagccgg ggtcggtggg
     1201 ctatctgttc agccagaagg gcctgatcga ggtgaagggc tctgaggacg aggtgatgct
     1261 cgtcgccctc gagtcaggtg ccgaggacat cagggacaac ggggacggct ggctggaggt
     1321 gatcacctcg cccggcgacc tgcaagccgt tcgctcgtca ctcgccgagg ccggcatcga
     1381 gatcgagtct gcggacgtga ctcaactccc gtcgaccact gtgccggtgg gagagggcga
     1441 cgcccgcaga ctccttcgtc tgatcgatgc cctcgacgac ctcgacgacg tccagtcggt
     1501 gtactccaac tacgacatcg aggacgacgt catggagaga gtcctcgaag aggtctgagg
     1561 atctcgggtc aggtgctggt cctgcgcccc g
//
LOCUS       DATITW010000804         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_198899, whole genome shotgun sequence.
ACCESSION   DATITW010000804 DATITW010000000
VERSION     DATITW010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198899"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VLS86_11235"
     CDS             complement(<1..210)
                     /locus_tag="VLS86_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HSO51112.1"
                     /translation="MTTTMEAPVPVFDEERSGRKLAIICSKGSLDMAYPGLVLANAAL
                     GEGIETHIFFTFWGFDMINKETMAHL"
     gene            complement(229..543)
                     /locus_tag="VLS86_11240"
     CDS             complement(229..543)
                     /locus_tag="VLS86_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012184598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HSO51113.1"
                     /translation="MSLSLIAGREVHVDQEGFMTEYDEWTDELAETLAANIDVQLTDP
                     HWKAIRFLREDFKAQGETPTLRRVTTVGGIPTKQLFELFPKKPAKKMAYIAGLPKPAG
                     CV"
     gene            complement(540..1814)
                     /locus_tag="VLS86_11245"
     CDS             complement(540..1814)
                     /locus_tag="VLS86_11245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HSO51114.1"
                     /translation="MNRLLVLGAGTAGTMVVNKLRPLLPREQWAITIVDQDSAHHYQP
                     GYLFVPFDPARAGDVVKPRQKFLTGDVDLILGQIDQVLPDDNVVRLSDGSELGYDYLV
                     IATGVSPRPDQTPGMLGEEWRKSIFDFYTLEGATALGEKLKTWDGGRLVVHITEMPVK
                     CPPAPLEFTFLADAFFKERGMRDRVEITFVTPLDGAFTKPVASNLLGGMLETRAITLE
                     PDFYIERVDDETKKIVSYDEREIPFDLLVTVPLNMGADFIARSGMGNELNCVPVDKHT
                     FLSPKWDNVFALGDAADLPTSKAGSVAHFAVEIFTENFLRHIAGEAMTDQFDGHANCF
                     VESGAGRAMLIDFNYDVQPLPGKYPVPGLGPMSLLVENRINHWGKLLFRWIYWHILLP
                     GRRLPVPTLMSMAGKRDVEPIAKSPTEKESVR"
     gene            complement(1940..>2797)
                     /locus_tag="VLS86_11250"
     CDS             complement(1940..>2797)
                     /locus_tag="VLS86_11250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSO51115.1"
                     /translation="NQRIALTEDTTLEELELDPIVTAEGTSLNVAMEGEGTVPLVLLH
                     DVDVAGSVIWDQVVSALDPKFRVMRVDLPGFGLSERIPVEGPRHTVASMAVEVGDAVE
                     QTFDQPVVIAGVGLGGEVAAEMAVINPDMVAAVVLIDVDFYKPDSWLQLVEKFPWLGT
                     AVTLAFETSGPFAAERWAPNCADGGWCPTQSQVEARDQAVVIVDTTNSMRAFRRTPAA
                     SVVPSKLNEITAPVHYLWSQQGDVPRESVDQVQEILPEAQFDVLADAWKAHLDLPRDV
                     AAAILAIAP"
BASE COUNT          496 a          877 c          894 g          530 t
ORIGIN      
        1 gaggtgggcc atggtctcct tgttgatcat gtcgaatccc cagaaggtga agaagatgtg
       61 ggtctctatg ccttcgccca gtgctgcgtt ggccaggacc agcccgggat acgccatgtc
      121 gagtgacccc ttcgagcaga tgatcgccag cttgcgaccg gatctctcct cgtcgaacac
      181 cggtactggt gcctccattg tcgtggtcat gtggtcaata cctccgaatc agacgcagcc
      241 ggccggtttt ggcaggcctg cgatgtaggc catcttcttc gccggtttct tggggaacag
      301 ctcgaagagt tgcttggttg ggatgcctcc cacggtggtc acccgacgca acgtcggggt
      361 ctccccctgg gccttgaaat cctcgcggag aaagcgaatc gccttccagt gggggtcggt
      421 gagctggaca tcgatgttgg cggcgagggt ctcagccagc tcatcggtcc actcgtcgta
      481 ctcggtcatg aagccctctt gatcgacgtg gacctcacgg cccgcgatca gcgacagact
      541 catcgcacac tctccttttc agtgggggat ttcgcgattg gctccacatc tcgcttcccg
      601 gccatcgaca tcagggtcgg gaccggcagt cttcgccccg gaagcaagat gtgccagtag
      661 atccagcgga acaggagctt gccccagtgg ttgatccggt tctccacgag cagggacatg
      721 gggccgaggc cgggcaccgg gtacttgccg gggagcggct gaacgtcgta gttgaagtcg
      781 atcagcatcg ccctccccgc gccggactcg acgaagcagt tggcatgacc gtcgaactga
      841 tcggtcatcg cctcgcctgc gatgtgtcgg aggaagttct ccgtgaagat ctcgacggcg
      901 aagtgggcca cggagccggc cttcgatgtc gggagatcgg cggcgtcgcc gagcgcgaac
      961 acgttgtccc acttcggcga gagaaaggtg tgcttgtcga ctgggacaca gttcaattcg
     1021 ttgcccatgc cactgcgggc gatgaagtcg gcgcccatgt tgagcggaac ggtcaccagc
     1081 aggtcgaagg ggatctcccg ctcgtcgtag gacacgatct tcttggtctc gtcatcgact
     1141 cgctcgatgt agaaatcggg ctcgagtgtg attgcccgcg tctcgagcat ccctccgagc
     1201 aggttcgagg caaccggctt ggtgaaggcg ccgtcgaggg gggtgacgaa ggtgatctcg
     1261 acccgatccc tcattccccg ctccttgaag aaggcgtcgg cgagaaaggt gaattcgaga
     1321 ggggccgggg ggcacttgac gggcatctcc gtgatgtgga ccacgagacg gcccccatcc
     1381 catgtcttca acttctcgcc cagggcagtc gcgccctcca gggtgtagaa gtcgaagatg
     1441 ctcttgcgcc attcctcacc gagcattccc ggggtctggt cgggccgggg actgaccccg
     1501 gtggcgatca cgaggtagtc gtagcccaac tcggagccgt cgctgaggcg gacgacgttg
     1561 tcgtcgggca gcacctggtc gatctgcccg agaatcaagt cgacgtcgcc ggtcaggaac
     1621 ttctgccggg gtttgaccac gtccccggcc cgggccgggt cgaacgggac gaagaggtat
     1681 cccggttgat agtgatgcgc cgaatcctga tctacgatgg tgatggccca ttgttcgcgg
     1741 ggaagcaggg gtcgcagctt gttgaccacc atggtcccgg cggtgcctgc gcccaatacc
     1801 agcagacggt tcatcggttg gctcctatca gaggcagatc tgcgctgccc tcccgaccag
     1861 catcgacggc agcggaaagc accgacaggg gcctaaggcc acacagttag ggtcctaaag
     1921 gcggtatcgc gggcgaggtc tacggcgcga tcgccagaat ggcggcggca acatcccggg
     1981 ggaggtcgag atgtgccttc caggcgtcgg ccaggacatc gaactgggcc tcggggagaa
     2041 tctcctgcac ctggtcgacc gactccctcg gcacgtcgcc ttgctgactc cacaggtagt
     2101 gaacaggcgc cgtgatctcg ttgagcttgg atggcaccac cgaggccgcc ggtgtcctgc
     2161 gaaaggccct catcgagttg gtggtatcga ctatgaccac ggcctggtcc cgcgcctcga
     2221 cctggctctg ggtcgggcac caacccccgt cggcacagtt cggcgcccaa cgttcggctg
     2281 cgaacgggcc tgacgtctcg aaggccaagg tgaccgcggt cccgagccac gggaacttct
     2341 ccaccagctg cagccagctg tcgggcttgt agaagtcgac gtcgatcagc accaccgctg
     2401 ccaccatgtc ggggttgatc accgccatct cggcggccac ctcaccaccc agtcccaccc
     2461 cggcgatcac caccggctga tcgaaggtct gctccacggc gtcgccgact tcgacagcca
     2521 tcgaggccac ggtgtgcctc gggccctcga ccgggatcct ctcgctgaga ccgaagccgg
     2581 gaaggtcgac tcgcatcacc ctgaacttgg gatcgagggc ggagacgacc tgatcccaaa
     2641 tcacacttcc ggcgacatcg acgtcgtgga gcaggaccag tggcaccgtc ccctcgcctt
     2701 ccatcgccac gttcagcgaa gtgccttccg cggtgacgat cggatcgagc tcgagctcct
     2761 cgagggtggt gtcctcggtc aacgcgattc gctggtt
//
LOCUS       DATITW010000805         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_319788, whole genome shotgun sequence.
ACCESSION   DATITW010000805 DATITW010000000
VERSION     DATITW010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319788"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1272
                     /locus_tag="VLS86_11255"
     CDS             154..1272
                     /locus_tag="VLS86_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51116.1"
                     /translation="MGARWWGRALLAVASVAGLVVILSVTEPRVPPVDWTRTVGPSGS
                     VNLDSLVATPDGFALLSGMTTEGVLLWSSADGSTWRSQPLQDAPSQLAVVDDGLIAYS
                     VRRGRMIEPTGEGWVEAADSLVFPDEVRSRQGSGRPSLIGTEDGLLAMSLFGDVWWSG
                     DGTQFDRVVADPDWGPGAELPFDSVCRPPIRTSPDVPPMVATDSGLVAMISSNLAEPF
                     GIWPVCEPQLWFSDDGQTWTDSDSTLGDGAYVFNLGWRDGRFTAVGGFGIGRPAAWTS
                     TDGRAWEPMAFFTDLEEVDLFTVESGAAGWVVLGRDSEGSETVGWTSSDGLCWNVLPD
                     SIGGGDAAVSAEHIIVFDRTTYPELWQGSTTGGNGACT"
     gene            1269..2915
                     /locus_tag="VLS86_11260"
     CDS             1269..2915
                     /locus_tag="VLS86_11260"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSO51117.1"
                     /translation="MTDSRSGAHILVESLLAEGVDLLFGIPGVGTLAVYDAFVDHPEL
                     RHIEVRHEQSAVFMADGYARASGGVGVAFTSNGPGALNTVTAMATAHNDSVPVLHLVS
                     ENPPEVRRKGKGHFHDISDQFSVFRPVSGYAAQVALADEIPAAVSSAFFALRNRRPRP
                     ALVEFANEAFTAASSATVLPRTERVERAGDSAAVTRAAAALAGASRPLVWAGGGIATT
                     AAAAELTRLVERLGAPVITTQKGKGAMSADHPLHIGNWANEQPVRDLIAGCDLVLAVG
                     TRFSYFPTGGWSLRLPERVVQIDIDPAEIGRNYRVETGVVGDAATVLGDIWSELDRLG
                     HEAAPWRSDDVAEVLARITEAVGTPVEVEVLEQIRSVLPPEALVFNDPTTIAFWARSL
                     WRSDRPRTWLVPSGFGTLGFALPASIGARLARPDTPSIAIIGDAGIMFTIQDLMTAVQ
                     EDIPAIVVVFNDEGYGVERRHQDHLYGRRSGVDVRPPDFVALAAAFGARGIKVDDLSR
                     VGSTIESVLDARGPTLIEVPNHFSHPGYGAFGSYKGRTSP"
BASE COUNT          452 a         1009 c         1007 g          508 t
ORIGIN      
        1 cccggaactg tctcttcgct ctccaccatc tgtcgtccac ggatcatata ggatgcacga
       61 tcgctcgaac cgcagcacct cctttcgaac cgagagccca tggccccttc accggaaatt
      121 ctcgtggaga tcccctctcc cgagagttct cccgtggggg cgcgctggtg gggacgcgcc
      181 ctgttggctg tcgcctcggt ggccgggctg gtggtgatcc tgtccgtcac cgagccccgg
      241 gtgccacctg tcgattggac gcgaacggtc ggcccatcgg gatcggtcaa cctcgacagc
      301 ctggtggcca cgcctgatgg cttcgcgttg ttgtcgggca tgaccaccga aggtgtgctg
      361 ctctggtcgt cggccgacgg ctccacatgg cgctcccaac ccctccagga tgctccgtcc
      421 caactggccg ttgtcgacga cggtctgatc gcgtacagcg tgcgcagggg gcggatgatc
      481 gagccgaccg gggagggctg ggtggaggcg gcggatagtc tggtgtttcc cgacgaagtc
      541 cgctcccgcc agggctcggg acgaccgagt ctgatcggaa ccgaggacgg gctccttgcc
      601 atgtcgctct tcggcgacgt gtggtggtcc ggtgacggga cgcagttcga cagggtggtg
      661 gccgaccccg actgggggcc gggggcggag ctgcctttcg actccgtgtg ccgtccaccg
      721 atccgcacct cgccggacgt cccgccaatg gtggcgaccg attcggggtt ggtggccatg
      781 atctcgagca acctcgccga gccgtttggc atctggcccg tttgcgagcc gcagctgtgg
      841 ttctcggacg acgggcagac ctggaccgac tcggattcca cccttggcga cggggcctac
      901 gtgttcaacc tgggttggcg ggacgggagg ttcaccgcag tcgggggctt tggcatcggc
      961 cgccccgcag cctggacttc caccgacggc cgggcgtggg agcccatggc cttcttcacc
     1021 gatctcgagg aagtcgacct tttcaccgtc gagtcgggag cagcgggttg ggtggtgctg
     1081 gggagagaca gcgagggctc ggagaccgtt ggctggacct cgtcggatgg tctgtgctgg
     1141 aacgtccttc ccgattcgat cggcgggggg gacgccgccg tttcggcaga gcacatcatc
     1201 gtcttcgacc ggaccacata ccccgagctg tggcaaggct cgaccaccgg cggaaacggc
     1261 gcctgtacgt gacagattcg cggagtgggg cccacatcct ggtcgagtcg ctcctggccg
     1321 agggagtcga cctcctcttc ggaatacccg gagtgggaac tctcgcggtc tacgacgcat
     1381 tcgtcgacca ccccgagctc cgccacatcg aggtccgtca cgagcagtcg gcggtgttca
     1441 tggccgacgg atacgcccgg gcgtcgggag gggtgggcgt cgccttcacc tccaacgggc
     1501 ccggggcgct caacaccgtc accgccatgg ccaccgccca caacgacagt gtccccgtcc
     1561 tgcacctggt gagcgagaac ccacccgagg tgcgtcgcaa gggcaagggg cactttcacg
     1621 acatatccga ccagttctcc gtgttccgcc cggtctccgg gtacgccgca caggtggcgc
     1681 tggccgacga gatcccggcc gcggtgagct cggcgttctt cgcacttcgc aaccgccgcc
     1741 cgcgtcccgc cctggtcgag ttcgcaaacg aggcgttcac cgccgcatca tcggccacgg
     1801 tgctcccccg gacggagcgg gtcgagcgtg ccggagactc tgcggcggtg acacgagccg
     1861 cggccgccct ggccggggcg agccgaccgc tggtatgggc cggcgggggg atcgccacca
     1921 cggccgccgc cgccgagttg acccggctcg tcgagcggct cggggccccg gtcatcacca
     1981 cccagaaggg caagggggcg atgagcgccg accacccgct gcacatcggg aactgggcca
     2041 acgagcagcc ggtccgggac ctcatcgccg gctgtgacct ggtgctggcg gtcggtaccc
     2101 gcttttccta cttccccacc gggggctggt cgctccgcct ccccgagcgt gtcgtccaga
     2161 tcgacatcga ccccgccgag atcggccgca actaccgggt ggagaccggt gtggtcggtg
     2221 acgcagcaac cgtcttgggg gacatctgga gcgagctaga tcggctgggc cacgaagctg
     2281 cgccgtggcg aagcgacgac gtggccgagg tcctggctcg gatcaccgag gcggtcggca
     2341 ccccggtcga ggtcgaggtc ctggagcaga tcaggtcggt cctgcccccg gaggccctgg
     2401 tgttcaacga cccgaccacg atcgccttct gggcccgctc gttgtggaga agcgatcgtc
     2461 cccgcacctg gttagttccc tccggtttcg ggaccctcgg attcgccctg cccgcctcga
     2521 tcggagccag actggcccgc cccgacaccc cgagcatcgc catcatcggc gacgccggga
     2581 tcatgttcac cattcaggac ctgatgaccg cggtgcagga ggacattccg gcgatcgtgg
     2641 tggtgttcaa cgacgaaggg tatggcgtcg agcgccgcca tcaggaccac ctctacggga
     2701 gacggagtgg agtcgatgtc agacctcctg atttcgtcgc tctggccgca gcctttgggg
     2761 cgagagggat caaagtcgac gacctctccc gggtcgggag cacgatcgag tcggtgctcg
     2821 acgcccgggg cccaactctg atcgaagtcc ccaaccactt cagccatccc ggctacgggg
     2881 ctttcggcag ctacaagggg aggacatcgc cttgaccacg atcgacatcc acgcccacgc
     2941 catcgtccct caggcgctga ccgagatggc tgcgag
//
LOCUS       DATITW010000806         1559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_119929, whole genome shotgun sequence.
ACCESSION   DATITW010000806 DATITW010000000
VERSION     DATITW010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119929"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VLS86_11265"
     CDS             <1..1114
                     /locus_tag="VLS86_11265"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSO51118.1"
                     /translation="LLRQAGETKAFGRDGYSSLTAMLKHRMSLHPGEALRHVTRANGL
                     AQAPLTSLAYARGAISGAQVDVLLEAKFVAGYAFSEAEGRLVTLAMDTPLVRDLKKHL
                     EYWLEQVAPVDLGAERNLMRDLRSLTLRRDGEMIRINGWVDIEAGERLRAGLELGPPA
                     EGDTRSTPARRADLLLDIMNGASGRPDITVHVAADALVEGSRGISETTSGTFLTIDEI
                     RRLACDANLTRVIFDPESRPLDVGRTKRLVTGPLRTAISARDLRCVFATCDRDANWCD
                     VHHLVHWADGGETNIDNLVLLCRSHHVLVHEAGWTITGQPGDLRFYRPDGTELGSEPP
                     PRPPYRSPRITVDTKRELPPGGLLPIILSVPRLRPP"
     gene            complement(1101..>1559)
                     /locus_tag="VLS86_11270"
     CDS             complement(1101..>1559)
                     /locus_tag="VLS86_11270"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HSO51119.1"
                     /translation="SDDFARWLGRAHKTARELMRTAPARLVPVAVEGWTGWMTTAGAA
                     GASTAGPVPGVYLLPGFDPYTLSPISHREQTIPPGRVNDVSRTAGWIAPVILDEGRII
                     GTWESGPRDTITLQPFGRVGSSTLAGLEHHVNDRYQGLLGADPRIQVREA"
BASE COUNT          262 a          533 c          487 g          277 t
ORIGIN      
        1 cctgctccgc caggcgggcg agaccaaggc ctttggtcgt gacgggtact cctcgctgac
       61 cgccatgttg aagcatcgga tgtccctgca tcccggagaa gccctgcggc atgtcacccg
      121 agccaatggg ctcgctcagg cgccactcac ctccctcgcc tatgcccgcg gtgccatctc
      181 gggtgctcag gtcgatgtgc tcctcgaagc gaagttcgta gctggctacg ccttttccga
      241 agccgaaggc cgactcgtga cgctggcaat ggacacaccg ctcgtccgag acttgaagaa
      301 gcatctcgag tattggttgg agcaggtagc tcccgtcgac ctcggcgccg agcggaacct
      361 gatgcgcgac ctgcgatctc tgactctgcg ccgggacggg gagatgatcc ggatcaacgg
      421 gtgggtcgac atcgaggccg gggagcgtct ccgcgccggg ttggagctcg ggccgccggc
      481 agagggtgac actcgatcca cgccggcgcg tcgtgccgac ctcctcctcg acatcatgaa
      541 cggagcgtcc ggccgtcccg acatcacggt ccatgtcgcc gccgacgctc tggtcgaagg
      601 gagccggggg atctccgaga ccaccagcgg gacctttctc acgatcgacg agatcaggcg
      661 gctcgcatgt gacgccaacc tgacccgggt catcttcgat cccgagtccc ggcccctgga
      721 cgtgggcagg accaagcgcc tggtcaccgg cccgctgcgg accgcaatct ccgctagaga
      781 cctccgatgc gtcttcgcca catgcgaccg cgacgcgaac tggtgtgatg tccatcacct
      841 ggttcactgg gctgacggtg gcgagaccaa tatcgacaac ctggtcttgc tctgtcgaag
      901 ccaccacgtt ctcgtccatg aggccggttg gaccatcacc ggccaacccg gagacttgcg
      961 gttctatcgg cctgacggca ccgagttggg aagtgagccc ccgccgcgac cgccatatcg
     1021 cagtcctcgg atcactgtcg acaccaaacg ggagcttccg cctggcggtt tgctcccgat
     1081 catcctcagt gttccccggc tcaggcctcc ctgacctgga tccgtggatc cgcccccagc
     1141 agaccctgat aacggtcatt cacgtgatgt tcgagcccag ccaaggttga acttccgacc
     1201 ctgccgaacg gttgcagcgt gatggtgtct ctcggcccgc tctcccaggt gccaatgatc
     1261 cggccctcgt cgaggatcac cggggcgatc cacccggcgg tccgggagac atcattgact
     1321 cgtcccgggg ggatggtctg ctcccgatgg ctgatcgggg agagggtgta ggggtcgaat
     1381 cctgggagta gatacacccc ggggaccggt cctgccgtag aggcaccggc agcgccggcg
     1441 gtggtcatcc atccggtcca accctcaacc gcaaccggga ccaatcgtgc cggcgccgtg
     1501 cgcatgagct ctcgggcggt cttgtgcgcc cgtcccaacc aacgggcgaa gtcgtccga
//
LOCUS       DATITW010000807         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_760285, whole genome shotgun sequence.
ACCESSION   DATITW010000807 DATITW010000000
VERSION     DATITW010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760285"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VLS86_11275"
     CDS             complement(<1..663)
                     /locus_tag="VLS86_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSO51120.1"
                     /translation="MAKPRHQVIVVGAGLAGLTAATRLLDAGLEVQVLEARDRVGGRV
                     WSHRLANGEMVELGGEWISTSQRAVIDLAHRLGLDLVDTGMDFISRDPIGAAPIPIEA
                     HDRLNRALADLMATLGTDTLEGMSVAKLLDRLDGHGPEMTVLRSRLGGTAGAGLDRVA
                     AAEIGVEFGIGDHGSYVRIQGGNDRLAKGLARHLDVRFEKAVSSVHQSEAEVEVVMRN
                     DVL"
     gene            717..>2689
                     /locus_tag="VLS86_11280"
     CDS             717..>2689
                     /locus_tag="VLS86_11280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HSO51121.1"
                     /translation="MTEPTPDYPVRIDRRVFVPMDDGERIALTIYLPDSSHDGPFPTV
                     VESLPYRKDDAFYSADWPTYAYLARRGIAGARIDIRGTGASSGVIEDEYLPREQEDTL
                     AVLGWLAEQSWCNGNLGMWGVSWGGFSALQTAMLRPPRLKAIAPVHATHDRFACDVHY
                     TGGSLHAQEQVDWPPSMVVCNALPPDPDIVGERWLDEWIERLERTPQWPGTWLRHQHR
                     DVYWLHGSPCADYESIQCPTLLIGGWVDGYVDGMLALAENLKCPTRTVIGPWGHYRPA
                     TGVPSPTLDHLDLLARWFGHHLRGDDNGVMDMPPLTLFIRTGAPYDGPTEPPFSDPPS
                     AGHWRVESSWPPGDTTTLETELAAMEHGATTWSGPQWVGVHAPAWDRAGIGSSDSTED
                     DAHSVCFETSPLTTPFEILGTPEVEVTVMSDKSVGMVAARLIAVAPDGTGHLISRGNR
                     NLVFPRDLSTPTPLVPDQPVTTRFPLLATSAVVPAGWRVRLALSGADFPVVWPPGERV
                     TLSFDPARSRLILPTVPHRPPETQLSIPTSPSPPSPPGVEGDEHMTKSVERSSSGVTY
                     RRTRSSTEFQPERADLTMTSEETWTISVEHDDPASTRVRSDAEVGMGRPGWAVTTRGS
                     LELSADAGFFHLAIDLTALHGEAVVFSRTWTEH"
BASE COUNT          452 a          975 c          834 g          428 t
ORIGIN      
        1 gaggacatcg tttcgcatca ccacttcgac ctccgcctct gactggtgaa cggatgacac
       61 ggccttctcg aaccgaacgt cgagatgccg ggccagacct ttggccaggc ggtcgttgcc
      121 cccctggatc cgaacatagg agccatggtc tccgatcccg aactccactc cgatctccgc
      181 cgccgccacc cggtcgagcc cggctcccgc cgtccctccg agacgagagc ggaggacagt
      241 catctccggg ccgtgcccgt ccagacggtc gagcagcttc gcaacggaca tcccctccaa
      301 agtgtctgtt cccagagtcg ccatgagatc ggccagcgct cgattgagcc ggtcatgggc
      361 ctcgatgggg atgggcgcag ctccgatcgg gtcccgtgag atgaagtcca tgccggtatc
      421 gaccagatcg agtcccagcc ggtgcgccag gtcgatcacg gcccgttgcg aggtcgagat
      481 ccactcgccg ccgagctcca ccatctcgcc attggccaga cgatgcgacc agacccgccc
      541 cccgacccgg tcccgggcct cgagaacctg gacctcgagg ccggcgtcga ggagacgggt
      601 ggccgcggtc aacccggcca gaccggcccc gacgacgatc acctggtgtc ggggtttggc
      661 catggggcgg gcaaggttac gcctggcgtg acgggcccgc tcgctagatt cccgccgtga
      721 ccgagccgac acccgactat ccggtccgca tcgatcgccg ggtgttcgtc ccgatggacg
      781 acggcgaacg aatcgccctc accatctact tgcccgattc ctcacatgac ggaccgttcc
      841 ccacggtggt cgagtccctc ccctaccgga aggacgacgc cttctactcg gccgactggc
      901 ccacctacgc ctatctggcc cggcggggaa tcgccggagc ccgcatcgac atccggggga
      961 ccggggcttc cagcggggtc atcgaggacg agtacctgcc cagagagcaa gaagacaccc
     1021 tcgccgtgct cggttggctc gccgaacaga gctggtgcaa cgggaacctg ggaatgtggg
     1081 gcgtctcttg gggagggttc agcgccctgc agacggccat gctgcgcccg ccacgactga
     1141 aagccatcgc cccggtccat gccacacacg accgctttgc atgcgacgtc cactacacag
     1201 gtggctcgct tcatgcccag gagcaggtcg actggccccc ttcgatggtg gtgtgcaatg
     1261 ccctgccccc ggatccggac atcgtcggag agcgatggct cgacgagtgg atcgaacgcc
     1321 tggaacgtac tccgcaatgg cccgggacct ggctccgaca tcaacatcgc gacgtttact
     1381 ggctgcatgg ctcaccctgc gccgactacg agtcgatcca atgcccgacc ctgctcatcg
     1441 ggggatgggt cgacggctac gtcgacggga tgctggccct cgccgagaat ctcaagtgcc
     1501 cgacccgcac cgtgatcggg ccctggggcc actaccggcc ggccaccgga gtcccgtctc
     1561 cgacattgga ccatctcgac ctcctcgccc gatggttcgg ccatcacctc cgcggcgatg
     1621 acaacggggt gatggacatg cccccgctca cccttttcat ccggaccggg gccccgtacg
     1681 acgggccaac ggagcccccc ttctccgacc cacccagcgc agggcactgg cgggtcgaga
     1741 gctcatggcc ccccggcgac acaaccaccc tggagaccga gctcgccgcc atggagcatg
     1801 gggcaaccac ctggtcgggc ccgcagtggg tgggtgtcca cgccccagcc tgggatcgag
     1861 ccgggatcgg aagcagcgac tcgaccgaag acgacgccca ctctgtgtgc ttcgagacga
     1921 gcccattgac cacccccttc gagatcttgg gaacacccga ggtcgaggtg acggtgatgt
     1981 ccgacaagag cgttgggatg gtggcggcgc gtctgatcgc cgtcgcaccc gacgggacgg
     2041 gccacctgat ctcgcggggc aatcggaacc tcgtgttccc ccgcgacctg tccaccccaa
     2101 caccgctggt gcccgatcaa ccggtgacca cgcgttttcc gctgctggcg acatcggcgg
     2161 tggtgccggc cgggtggcgg gtccggctcg ccctgtccgg ggctgacttc cccgtcgtct
     2221 ggccgcccgg ggagcgggtg acactgtcct tcgaccctgc ccgctcgcgt ttgatcctgc
     2281 ccacagtgcc ccatcgcccc ccggagaccc agctttccat ccccacgtct ccctcaccac
     2341 cgtcgccgcc tggagtcgaa ggcgacgagc acatgaccaa gtcggtcgag cgcagctcgt
     2401 ccggtgtcac ctaccggcga acgcggagct cgaccgagtt ccagccggag cgagcagatc
     2461 tgaccatgac ctctgaggag acctggacga tcagcgtcga gcatgacgac cctgcatcga
     2521 cccgggtccg ctccgacgcc gaggtcggga tgggccgccc gggatgggcg gtcacgacca
     2581 gaggcagtct cgaactgtcc gctgacgccg ggttcttcca cctggcgatc gatctcaccg
     2641 ctctacacgg cgaggcggtc gtgttctcca ggacctggac cgaacacat
//
LOCUS       DATITW010000808         1690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_39640, whole genome shotgun sequence.
ACCESSION   DATITW010000808 DATITW010000000
VERSION     DATITW010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39640"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..710
                     /locus_tag="VLS86_11285"
     CDS             273..710
                     /locus_tag="VLS86_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSO51122.1"
                     /translation="MELKVWTPFLDLDKEWRLFDFPRLIGEGREFPLRPSIDIVRKNG
                     DLVVTAELPGLDPEKDIEISVEGGVLSIKGEKTEEKEVSEDNRYMHERSYGKFSRRIS
                     LPGGVSPDKVTADYDKGVLTVHVGLPTEKAEERRTIPVKVKTA"
     gene            complement(799..>1690)
                     /locus_tag="VLS86_11290"
     CDS             complement(799..>1690)
                     /locus_tag="VLS86_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HSO51123.1"
                     /translation="STARAAAQVARMQGATRVVVATPVAPVEVRDGLGGDVDEVIVVE
                     TPYPFYAIGQFYLDFSPTTDDEVAAILYRAGSISSPASVEGPPPVDEEVVVGPIGLGG
                     HLTIPEGALGTVVFAHGSGSSRHSPRNRHVADELNRVGLGTLLFDLLTPIEESDRGNV
                     FDIELLGRRLVDGTRWLTGREGWGNHPVCYFGASTGAAAALWAAAELGDAISAVVSRG
                     GRPDLAGSRLPAVRAPTLLIVGGLDRVVLDMNRAAQAQLTCENRLDVVPGASHLFEEP
                     GALDQVADLARDWFVSHCRS"
BASE COUNT          330 a          555 c          540 g          265 t
ORIGIN      
        1 cccatttcgc ctgctactac ttgggaagtc ttgcgcctag ataggaccga tcctgcagca
       61 cggcggtacc acaggataat cccaatccga ccgctgccgg aatcccgcgt gcatggagtc
      121 cagcccgcgc cggggaccgc aaacccaaga gagatcgggt cgttcggccc tgttcaagcc
      181 ggtcggtgag ttggatgatc gtgatagaga cccggcgggt ccgggccacc tgcctgtttg
      241 tgggcccagg cccgagaacg gaggtgaaac agatggaact caaagtgtgg acacccttct
      301 tggacctaga caaggagtgg aggttgttcg acttcccgag gctcatcggc gagggacgag
      361 agttcccgct tcgcccatcc attgacattg tcaggaagaa cggcgacttg gtcgtgaccg
      421 ccgaactgcc cggtctcgat cctgagaagg acatcgagat ctcggtggaa ggcggtgtgc
      481 tctccatcaa gggagagaag accgaggaga aggaagtctc tgaggacaat cgttacatgc
      541 acgagcgaag ctacgggaag ttctcgcgtc gcatctccct gcccggtggc gtcagtcccg
      601 acaaggtgac tgccgactac gacaagggag tactcaccgt gcatgtcggt ctgcccacgg
      661 agaaggccga ggagcgtcgc acgatcccgg tgaaggtgaa gaccgcctga gctggggcat
      721 ccctgctgtg agaggcgagc caacgattgg ctcgcctctc agttcgaccg ctccgtccgc
      781 aagctgccgg cggagatctc aggatcggca gtgggagacg aaccagtccc gggcgagatc
      841 ggcaacctga tccagggcac ctggctcttc gaagagatgg ctggcgccgg ggaccacgtc
      901 cagtcggttt tcgcaagtga gctgggcctg ggcggcccgg ttcatgtcga gcacgacccg
      961 gtccagacca ccgacgatga gcagcgttgg agcgcgtacc gcggggagac gcgacccggc
     1021 cagatcgggc cgaccgcccc tcgagaccac cgccgagatc gcatcaccca gttcggcggc
     1081 tgcccacagg gcagcagcgg cgccggtcga agcgccgaaa tagcagaccg ggtgattacc
     1141 ccacccttcc cgcccggtga gccaccgggt cccgtccaca agacgccggc ccagaagctc
     1201 gatgtcgaac acattccccc tgtcggactc ctcgatcggg gtcagcaggt cgaagagaag
     1261 agtccccaga cctactcggt tcagttcgtc ggccacatgt cggttgcgcg gactgtgacg
     1321 gctgctcccc gaaccgtggg cgaacaccac cgtccccagc gcaccttcag ggatcgtcag
     1381 gtgccctccg agcccaatgg gaccgaccac cacctcttcg tcgaccgggg gaggtccctc
     1441 gaccgatgcc ggcgaggaga tcgaccccgc ccgatacaga atggcggcca cctcgtcgtc
     1501 cgtcgtcggg gagaaatcga ggtagaactg gccgatggca tagaacggat aaggggtctc
     1561 cacgacgatc acctcgtcca cgtccccgcc cagcccgtcc ctgacctcca ccggggcgac
     1621 cggcgtggcc accaccaccc gggtcgctcc ctgcatccgg gccacctggg ccgcggccct
     1681 ggcggtcgag
//
LOCUS       DATITW010000809         1580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_800101, whole genome shotgun sequence.
ACCESSION   DATITW010000809 DATITW010000000
VERSION     DATITW010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800101"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1552)
                     /locus_tag="VLS86_11295"
     CDS             complement(<1..1552)
                     /locus_tag="VLS86_11295"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HSO51124.1"
                     /translation="MLLALGAVHQHLIDQGVRGSVSLVVVSGEPRDPHDLACLVGFGA
                     SAVNPYLAIEHARDLAASGAIEVDPVPAQENFRLAMEGGLLKIMSKMGICTLSAYRGS
                     ELFEVIGLSEAVCRKAFRNAPRRLRGIGLKELARQALQRHEAYAAGAPESGGFYKHRR
                     GEDNHVTSPMVVLAVQKAVRSGDPEDWDRYIEMISDREPALVRDLLTFVQRDPVPIDE
                     VEPVEQIMRRFTTAAMSLGALSSEAHIALAEAMNLIGGHSNSGEGGEDPARFDTPKNS
                     AIKQVASGRFGVTPAYLDSADELQIKMAQGSKPGEGGQLPGHKVSPEIARLRYTEPGV
                     TLISPPPHHDIYSIEDLAQLIYDLKAFKPTARVSVKLVSEPGVGTIAVGVAKADADVI
                     TISGSEGGTGASPLVSIKHAGSPWELGLAEAHQVLTANGQRHRVLLETDGGLRTGRDI
                     VVAALLGAERFGFGTRPLLALGCKMVRQCHLNTCPVGIATQNEDLRAKFTGSADQVVL
                     LFRRLAEEA"
BASE COUNT          274 a          504 c          536 g          266 t
ORIGIN      
        1 gagcctcctc ggcaagacga cggaatagga gaacgacctg atcagccgag cccgtgaatt
       61 tggcccggag atcctcgttc tgggtggcga tgcccaccgg gcaggtgttc aagtggcatt
      121 gacggaccat cttgcagcca agtgcgagca ggggacgggt gccgaagccg aatcgctccg
      181 cccccagcag cgcagcgacc acgatgtcgc gtccggtgcg cagcccgccg tcggtttcca
      241 gcaggacccg atgtctttgc ccgttggcag tcagaacctg atgcgcttcg gcgagaccga
      301 gctcccacgg cgagccggcg tgtttgatcg agaccagcgg cgaggcaccg gtgccgcctt
      361 cggagcctga gatcgtgatg acatcggcgt ccgccttggc cactcccacc gcgatcgtcc
      421 ccactccggg ctcgctgacg agcttcaccg agacccgggc cgtgggtttg aacgccttga
      481 ggtcatagat cagctgggcg aggtcctcga tggagtagat gtcgtgatga gggggcggcg
      541 agatgagggt gacgcccggc tcggtgtagc gaagacgggc gatctccggg gagaccttgt
      601 gcccgggaag ctggccacct tcgcccggtt tggagccctg ggccatcttg atctggagct
      661 cgtcggcgga gtccaggtag gcgggggtga cgccgaaacg tccggaggcg acctgcttga
      721 tcgcggagtt cttgggggtg tcgaagcgcg ccgggtcttc gcctccctcg cccgagttgg
      781 agtggccgcc gatcaggttc atggcctcgg cgagtgcgat atgtgcctct gaggacaagg
      841 cgccgagcga catcgcggcc gttgtgaagc gacgcatgat ctgctccacc ggctccacct
      901 catcgatggg gaccgggtcc cgttgcacga acgtcagcag atcgcgcacc agcgccggtt
      961 cgcgatccga gatcatctcg atatagcgat cccaatcctc agggtcgccc gagcgcactg
     1021 ccttttgcac cgccagcacc accatcgggc tggtgacatg gttgtcctcc ccgcggcgat
     1081 gcttgtaaaa gccgccggac tcgggcgcgc ctgccgcata ggcctcatga cgttgcaggg
     1141 cctggcgggc cagctccttg aggccgatcc ctcgcagccg acggggtgcg ttgcggaagg
     1201 ccttgcggca aaccgcctcg gagagaccga tcacctcgaa caactccgaa ccgcggtagg
     1261 cggaaagggt gcagatcccc atcttggaca tgatcttgag cagaccaccc tccatcgcga
     1321 gacggaagtt ctcctgagcc ggtaccgggt cgacctcgat cgcccccgac gccgccaggt
     1381 cgcgggcgtg ctcgattgcc agataggggt tgaccgcgga ggccccgaag ccgacgaggc
     1441 aggcgagatc atgggggtca cgcggctcac cggacacgac caccagcgaa accgagccac
     1501 ggaccccttg gtcgatcaga tgttggtgga cggcgcccaa ggcgagcagc atcggaattg
     1561 gtgctcgctt ggcatcggtc
//
LOCUS       DATITW010000810         1884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_720975, whole genome shotgun sequence.
ACCESSION   DATITW010000810 DATITW010000000
VERSION     DATITW010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720975"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            611..1228
                     /locus_tag="VLS86_11300"
     CDS             611..1228
                     /locus_tag="VLS86_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51125.1"
                     /translation="MNLRDFEEKVRRLPGVEAARVVTEGGAVTEVHVLTGMGKAAKQV
                     VRDVQSLAQAAFGLAIDRRVVSVVQLPDPDLAQGDRPVIVDVSEQMDGNHTKVVVTLD
                     WQGDTLVGEVAGAAAHTTRNRLVAEATIEALQQALQETTAIGVAAVDLPVLGSRSVAI
                     AQVVMVTEGAERLMVGSALVDGDGSRAVVRAVLDALNRHIPELRR"
     gene            1317..>1884
                     /locus_tag="VLS86_11305"
     CDS             1317..>1884
                     /locus_tag="VLS86_11305"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HSO51126.1"
                     /translation="MVATGGNAVDAAVAANAVLGVVAPETCGIGGDLFALVHRPGMQR
                     PDVLNSSGRAGAGADPDKLRTQGHRTMPPFDPQTITVPGCVDGWEALLARYGSKSMPA
                     VLAPAIGLASDGFPASFELVGALTRHPELAHQTSARVLYPEGNPPAIGEKLRRPDLVS
                     TLETIAAMGRNGFYLGPAGQGIMKATGGV"
BASE COUNT          358 a          611 c          581 g          334 t
ORIGIN      
        1 gaccgtcgaa gacggcggat gcagcgagca cgccgaggcc agccagcacg taaccggtgg
       61 cgagcacgaa tcgtcgtcga gcaagacctg tcatctgatt tgaagtcctg ggggtttagc
      121 gcgcagtttt tggcgctgct gttttagcta tggggggcgg agaaccggcg acaatccctt
      181 cagccgatct tcatagaggc agaaccagca acgatcaatg ccgtcaggct tcccagagca
      241 gcaacgatcc gaacgatctt gcccttcatg cgatcaccag tttcgttttt aggttcaccg
      301 tggccgctaa caaccaggtg gacccgatcc gctccgctgg ggtgattaaa caaatggcct
      361 cagggcgtgt cgcccggttc cccgcgcgtt cccggtttta tccgggactc ttgttgtcaa
      421 gtgaagactt tctcgccggg cgaacttgcg accgtacttg caaccccaag actctgccca
      481 accggaccgt atatattaca agcggcgggt ggggccaaca aggccacctg gtcttgctct
      541 accacccacc tcacaccgta acactttaag tgacacatca ccaccgtccg cggcggctac
      601 gcttcgaatt gtgaaccttc gtgacttcga agagaaggtc cgtcgattgc ccggcgtcga
      661 agcggcccgg gtcgtcaccg aaggcggagc ggtgaccgag gtccacgtcc tgaccgggat
      721 gggcaaggca gccaagcaag tcgtccgaga tgtccagagc ctggcccagg cagcgttcgg
      781 actagccatc gaccgcaggg ttgtatcggt cgtacaactt cccgatccgg atcttgctca
      841 gggggatcgc cccgtcatcg tcgacgtgtc cgagcagatg gatggcaatc acacgaaagt
      901 cgtggtcacc ctcgattggc aaggcgacac gctggttggc gaagtcgcag gagccgcggc
      961 tcacacgacc cgcaaccgcc tggtcgccga agcgacgatc gaggcccttc aacaggccct
     1021 gcaggagact acggccatcg gagtcgccgc ggtcgaccta cccgtcctcg gctcgaggtc
     1081 ggtggcgatt gcccaggtcg tcatggtcac cgaaggggcg gagcggctca tggtcggctc
     1141 ggccctcgtg gacggggacg gcagccgcgc tgtcgtgcga gcagtgctcg acgccctcaa
     1201 tcgccacatc cctgagcttc gccgctgagg tggaatcgct tcgcagtcat gccctccacg
     1261 ggtacggggc tgccgcggca actccgcatg tcctagcgac ccaggctgcc atggccatgg
     1321 tcgcgaccgg gggaaacgca gtcgatgccg ccgtcgccgc caatgcggta ctcggagtag
     1381 tcgcgccaga gacatgtgga atcggaggcg atctgttcgc cctggtccac cgacccggaa
     1441 tgcaacgacc cgatgttctc aactcgtccg gccgggccgg cgccggtgcc gaccctgaca
     1501 agctccgaac gcagggacac cggacgatgc cgccttttga tcctcagacg atcaccgttc
     1561 ccggatgcgt cgacggctgg gaggcgctgc tcgcccgcta cggcagcaaa agcatgccgg
     1621 ccgttctggc cccggcgatc ggattagcct cggacggctt tccggcttcg ttcgaactgg
     1681 tcggagcctt gacaaggcat ccggagttgg cgcaccagac ctccgcccgg gtcctctacc
     1741 cagaaggcaa tcccccagcc atcggtgaga agctccggcg ccccgacctc gtatccaccc
     1801 tggagaccat tgcagccatg ggccggaatg gcttctatct gggtccggcc gggcaaggaa
     1861 tcatgaaagc aaccggaggg gtca
//
LOCUS       DATITW010000811         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_239959, whole genome shotgun sequence.
ACCESSION   DATITW010000811 DATITW010000000
VERSION     DATITW010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239959"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2426)
                     /locus_tag="VLS86_11310"
     CDS             complement(<1..2426)
                     /locus_tag="VLS86_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSO51127.1"
                     /translation="MKSLKLVGFKSFADRTRLEFRSGVTVIVGPNGSGKSNLVDALSW
                     VMGSQSTKALRTGKMEDVVFAGTATRPSLNRAEATLIIDNAERMLPLDLDEVALTRRL
                     FRDGSSDYEINGVGCRLLDVQELLSDSGVGRHQHVIIGQGQVDGVLNASPEEHRAVVE
                     EAAGILKHRRRKEKAERRLERTDEDVLRLQDLLGEIERQLRPLRRQARAAEVYDGLKS
                     EVRELHLYLSGLSLQAYDTELGEGRGTVERLTAETSADDAAVGRLAASFEELTATAAA
                     VGADLDRDTGAAARLETTIERLRRLGSVAHERNRAVTGRNEASVERRADLQAELEEIR
                     AQIAEIAGSWSEATVDVEAREARFRLLEDEERSIATQTAHSPEGALAAVRGELSVLQA
                     SAVRDAREIEAIEHRLGVVASQIRDESTEISQINEEVRAVDEVVSGLQGAYQKTNRTR
                     EADQERWSTAEAALNEARMHVAAAEARVEAVAAALAGRFDLEARRSVEDAAGALGSLV
                     SRLDIPPGLEKAVDAALGRWVDAVAFEDVAALQMAVEGVKTGGGGGVPVVSALTAAST
                     PASEMAAATGVEALVDRLGPQAHRALASRLLGDVVLVEGWTSAWALVSRHPALRAVTP
                     EGDLVSVDGIQIAHPDGAGPAMLEASEVAAEQARTALSRAVSIHTAAKRDFEKGRDLE
                     RSALEELETAEAGLAGKTEAMARLQKSVDVLEGERQRLMQREAAIRDGATERERQAAE
                     LTRRLQALEGEEAERMKMWEELETKRIQIAADREMARSEWQDAASVLRGISERRHLLE
                     ERLGRIEVELG"
BASE COUNT          411 a          962 c          813 g          435 t
ORIGIN      
        1 ccgagctcga cctcgatgcg ccccagtcgc tcctcgagca gatgacgacg ctcgctgatc
       61 ccacgaagga ccgatgcggc atcctgccac tcggaacggg ccatctcccg gtcggcggcg
      121 atctggatcc gcttcgtctc cagctcctcc cacatcttca tcctctccgc ctcttcgccc
      181 tccagggcct ggagcctgcg ggtgagctcc gcggcctgac gctcgcgctc ggtggcgcca
      241 tcacgaatcg cggcctctct ctgcatgagc cgttgtctct cgccctcgag gacgtcgact
      301 gatttctgga gtctcgccat cgcctcggtc ttgccggcca atccggcctc ggcggtctcc
      361 aactcctcga gagccgaacg ctcgagatcg cgccccttct cgaaatctct cttcgccgcg
      421 gtgtgaatcg acaccgcgcg ggagagagcg gttcgggcct gctccgcggc cacttctgag
      481 gcctcgagca tcgccgggcc cgccccgtcg ggatgggcga tctgaatgcc gtcgaccgag
      541 acgaggtcgc cttccggagt caccgcccgc agcgcagggt gacgggagac gagcgcccac
      601 gccgaggtcc agccctcgac caggacgacg tcgccgagca gacggctggc cagtgcccgg
      661 tgggcctgcg gaccgagacg gtcgaccagt gcctcgacac cggtcgcagc cgccatctcg
      721 gatgcgggcg tggaggcggc tgtcagcgcc gagacgacgg ggacaccccc gcctcctccc
      781 gtcttgacgc cctccacggc catttgcagg gcggcgacgt cttcgaaggc cacagcgtcg
      841 acccagcggc cgagggcggc atcgaccgcc ttctccagac ccggtgggat atcgagccgg
      901 gagaccaggc ttcccagtgc cccggcggca tcctccaccg accgccgggc ctccagatcg
      961 aagcgaccgg ccagggcggc tgcaacggcc tccacccgag cctcggcagc ggccacgtgc
     1021 atccgggcct cgttgagcgc agcctcggct gtggaccatc tctcctgatc cgcctcgcgg
     1081 gtccggttcg tcttctgata ggcaccctgc agaccggaca ccacctcgtc gacagcgcgt
     1141 acctcttcgt tgatctgtga gatctccgtc gactcgtccc ggatctgaga ggccacgacg
     1201 ccgagccgat gctcgatcgc ctcgatctca cgggcgtccc ggacggccga ggcctggagg
     1261 acggacaact cgccccggac cgcggcgagc gcaccctcag gagagtgcgc ggtctgggtc
     1321 gcgatcgaac gctcctcgtc ctcgagaagc cgaaaccggg cttcacgtgc ttcgacgtcg
     1381 acggtggctt ccgaccagga ccctgcgatc tctgcgatct gagcccgaat ctcctccagc
     1441 tcggcctgga ggtcggcccg acgctcgaca cttgcctcgt ttcgaccggt caccgcccga
     1501 tttcgctcgt gggcaacgga tcccagacga cgcagtcgct cgatggtggt ctcgagcctc
     1561 gccgcggcgc ccgtgtcccg gtcgagatcg gcccccacgg ctgcggcagt ggcggtcagc
     1621 tcctcgaacg aggccgccag cctgcccacc gcggcatcat ccgccgaggt ctcggcggtg
     1681 agccgctcga cggtccccct gccctcgccc aactcggtgt catacgcctg gagagacaga
     1741 ccggacagat acaggtgcag ctccctgacc tcggacttca gcccgtcgta gacctcagcg
     1801 gcccgcgcct ggcggcgcag gggccgtagc tgtcgctcga tctcgcccag gaggtcctgg
     1861 aggcgaagca cgtcctcgtc ggtccgctcc agacgccgct ctgccttctc cttgcgccgc
     1921 cggtgcttga ggatgccggc cgcctcctcc accaccgccc gatgctcctc gggtgaggcg
     1981 ttgaggactc cgtcgacctg tccctgcccg atgatcacgt gctgatgacg tcccacaccg
     2041 gaatcgctga gcagttcctg cacgtcgagc aaccgacaac cgactccgtt gatctcatag
     2101 tcactcgatc cgtcccggaa cagccgtctg gtcagggcca cctcgtcgag atcgaggggg
     2161 agcatgcgct cggcgttgtc gatgatgagc gtggcttcgg cgcgattcag cgaaggcctg
     2221 gtggcggtgc cggcgaagac gacgtcctcc atcttcccgg tgcgcagcgc cttggtcgat
     2281 tgggagccca tgacccagga caacgcatcg accaggttcg acttcccgga gccattcggc
     2341 cccacgatca cggtcacccc ggaccggaac tcgagtctgg tccggtcggc gaacgacttg
     2401 aaaccgacga gcttcagtga cttcaaatac acagcaaatt cctggcgaat gacacgactg
     2461 tgattatccg atcaggctaa cagcgccggg ggcatcagcc gctcttcatc gggttgggat
     2521 ggatgaagaa gtcgcgaagg gtgcggtcgg cggcgacgac ttcggactcg acaccgacga
     2581 ccaccgccat cgccgggccg ccccaaagcc agctcacgag c
//
LOCUS       DATITW010000812         1974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_400117, whole genome shotgun sequence.
ACCESSION   DATITW010000812 DATITW010000000
VERSION     DATITW010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400117"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VLS86_11315"
     CDS             complement(<1..537)
                     /locus_tag="VLS86_11315"
                     /inference="COORDINATES: protein motif:HMM:NF016347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HSO51128.1"
                     /translation="MGRHFPHLLVEGDWDHDTLGLTRDQLHHLEKVLRILEVADVSNT
                     NGRGTVANGVFSGGLIVRDREETVPRPYPITLAVAPPASRDRCRFLVEKLSELGVARL
                     VWLATSRGEGRIPHAAKTTLWAASALEQSRGGWLMEVGAALLSWDDLDRPLVVAIPGG
                     GSEIFEVRTVAIGPEGGLE"
     gene            complement(537..1610)
                     /gene="dnaJ"
                     /locus_tag="VLS86_11320"
     CDS             complement(537..1610)
                     /gene="dnaJ"
                     /locus_tag="VLS86_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HSO51129.1"
                     /translation="MARDYYSILGVGRDATTEEIKKAFRRLARATHPDANPGDDGAEA
                     KFREAAEAYEVLVDPERRRRYDRGDTIDLSDLFSGFGGFDDILRSVFGDGGMFGSQSG
                     RSNRGRDILVRVEVDLASAAFGTDASVSFHTKTTCLQCGGSGAEAGSGQSTCPDCGGS
                     GSVRTARRSLFGTMMTVGSCPTCRGEGVLIVEPCQSCSGAGSVPEDTTVTVEVPAGVS
                     AGSRLRLSGRGESGGRSGPPGDLYVEIVVAPDPRFDRVDSDLIHRVTLGIAEATLGSR
                     VEIPLLEGGVTELEIPPATQPGTGFRLAGLGVPRLGRRSRGDLQVLVEVRIPDQLSAE
                     EETLLRQWSDLRGERTDRPASAG"
     gene            complement(1615..>1974)
                     /locus_tag="VLS86_11325"
     CDS             complement(1615..>1974)
                     /locus_tag="VLS86_11325"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51130.1"
                     /translation="IADGLHLAASSGAEVFVGGAQRLSSVWADLSAVQRVLEVLEREA
                     EVMRILAGASGTSIQIGAQLGGDVDLAVVSKSYEAGSAGGSVGVIGPMRMNYKRAISA
                     VEEVSRELEGRISSKPD"
BASE COUNT          361 a          714 c          591 g          308 t
ORIGIN      
        1 ctccagcccg ccctcgggcc cgatggcgac cgtcctcacc tcgaatatct cgctcccacc
       61 tccgggtatg gccaccacca gcggccggtc tagatcgtcc caggacagca gggcggcccc
      121 gacttccatc agccagccgc ctcgcgactg ctccagggca gacgccgccc agagagtggt
      181 cttggcggcg tgggggattc gaccctcgcc acggctggtg gccagccata ccaggcgggc
      241 tacacccagt tcggagagct tctccaccag aaaccggcaa cgatcccggc tggccggtgg
      301 ggccacagcc agagtgatcg ggtagggcct ggggacggtc tcttcacggt cacgaacgat
      361 caggccccct gagaacaccc cgttcgcgac agtgcctcgc ccgttggtgt tgctgacatc
      421 cgcaacctcc aaaatccgga ggaccttctc cagatggtgg agttggtctc gggtcagtcc
      481 gagcgtgtcg tgatcccaat cgccttcgac caggagatgc ggaaaatgac gacccatcag
      541 ccggccgacg cggggcgatc ggtgcgctca cctctcaagt ccgaccactg gcgcagcaaa
      601 gtctcctcct cggcgctgag ctggtccggg attcggacct cgaccagcac ttggaggtcg
      661 cctcgactcc tcctccccaa ccgaggcacg ccgagccccg ccagcctgaa gccggttccg
      721 ggctgggtgg cgggcgggat ctcaagttcg gtgacaccac cctcgagaag agggatctcc
      781 accctcgacc ccagggtcgc ctcggcgatg cccaaggtca ctcgatgaat gaggtccgaa
      841 tcgacccggt cgaaccgagg gtccggagcc accacgattt cgacatagag atcaccgggt
      901 gggccggagc gcccacccga ttctccgcga cccgacaggc gaagccgcga cccggcagaa
      961 accccggcgg ggacctcgac ggtcaccgtg gtgtcctcgg gcaccgagcc tgccccggaa
     1021 caggattgac aaggctcgac gattagcacc ccttcacccc gacaggttgg acagctgccc
     1081 accgtcatca tcgtcccgaa caacgacctc cgcgcggtgc gtaccgaccc actgcccccg
     1141 cagtcagggc aggtcgactg gccggaaccc gcttcggcgc cactcccccc gcactggaga
     1201 caagtggttt tggtgtggaa cgacaccgag gcatcggtcc cgaacgctgc cgacgccaga
     1261 tcgacctcaa ccctgaccag gatgtcccgg ccccggttgg agcggcccga ttgggagccg
     1321 aacatccctc cgtcgccgaa caccgagcgc aagatgtcgt cgaagccacc gaaaccggag
     1381 aagagatcag agagatcgat ggtgtcgccc cggtcgtaac gacgtcgccg ctcggggtcg
     1441 accagcacct cgtaggcctc ggccgcctcc cggaacttcg cttcggcccc gtcgtcgccg
     1501 gggttggcat cggggtgggt ggcgcgcgcc agccgacgga atgccttctt gatctcctcg
     1561 gtggtcgcgt ccctaccgac cccgagtatg gagtagtaat ccctggccat ctactcaatc
     1621 gggcttcgag ctgatccggc cttctagctc tcgggagact tcttccaccg ccgagatcgc
     1681 ccgtttgtag ttcatgcgca tcggaccgat caccccgacc gaccctccag cgcttcccgc
     1741 ctcgtaggac ttggacacga ccgccagatc gacgtcacca cccagttggg caccgatctg
     1801 gatcgaggtg cctgaggcac cggcgaggat cctcatcacc tcggcctcgc gttcgagcac
     1861 ctcgagcacc cgttgcactg cggaaagatc agcccaaacg ctggagagcc gctgtgcacc
     1921 cccaacgaac acctcggccc ccgacgaggc agcgagatgg aggccatcgg caat
//
LOCUS       DATITW010000813         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_199683, whole genome shotgun sequence.
ACCESSION   DATITW010000813 DATITW010000000
VERSION     DATITW010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199683"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..565)
                     /locus_tag="VLS86_11330"
     CDS             complement(116..565)
                     /locus_tag="VLS86_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51131.1"
                     /translation="MARWIALSLMALAVVSCAGDGNPATTTVAETTTVPPTATSSVAT
                     TTPATTPATTTTTDAPSTSAQQIDVYFEGGQVVGPDRVQHALGDLVSVWVLSDVDDQA
                     HVHGYDLFFAAKAGVPLEISLTADIPGIFEVELESTHTLLFELEVTP"
     gene            626..>2188
                     /locus_tag="VLS86_11335"
     CDS             626..>2188
                     /locus_tag="VLS86_11335"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HSO51132.1"
                     /translation="MRWVRRILVALLLVAVAIGVFGVFLVRRSFPHVEGELTLAGLDD
                     FVEIIRDEDGVPHVYATTGHDLFFAQGFVHAQDRFWQMDFWRHIGAGRLSEMFGDGQV
                     ETDMFLRSLGFTDLAAEELESMTPELVAILEAYSEGVNAYLADRSPSQVSLEYGILPL
                     QASGYEIEPWTAVNTLTWAKVMSWDLSWNLLQEIDRATLSSRLPLERIEQLYPGYPEA
                     NPVVVASDQTASVSRTGPTIPPAAREALTAAGQRAGLAWTMTGGGFAGIGSNNWVVGG
                     SRTESGLPILANDPHLAIQMPSIWYQNGLHCVEITTDCPFQVAGFSFAGSPGVIIGHN
                     DRIAWGVTNESVDTQDLFIERLNPDDPAQYEYEGEWVDMEVRAETIEVAGGENVTYDV
                     MSTRHGPIISDLYLDEGQFDDSSLDLPDEYAVSLSWQTLEPSTLVEAIVGLNRATGYD
                     DFRTALSKWDIAAQNVVYADVEGNIAYQSTGEVPIRAAGDGRWPMPGWTGEHEWIGVV
                     PFEDMPSLLNPSP"
BASE COUNT          381 a          682 c          749 g          376 t
ORIGIN      
        1 tagctgaccg gaaccgggag atcgagacgc ccgccgatcc cgtgggccag ggccagggac
       61 gggatggcca ggacgatgcc gacagcaatc agggatgaga cgccgaggcg tctgatcaag
      121 gagtcacctc gagctcgaag agaagggtgt gcgtcgattc caactcgact tcgaagatcc
      181 ctgggatgtc cgccgtcagt gagatctcca gtgggactcc ggccttggcg gcgaagaaga
      241 ggtcgtagcc gtgcacatgg gcctggtcgt cgacgtcgga gaggacccag accgacacca
      301 gatcaccgag tgcgtgctgg acgcgatccg ggcccaccac ttgccccccc tcgaagtaga
      361 cgtcgatctg ctgggccgag gtcgacgggg cgtcggtggt ggtggtcgtc gccggggtgg
      421 tggcgggggt cgtcgtggcc acgctcgagg tcgcggtcgg ggggacggtc gtggtctcgg
      481 cgaccgtcgt cgtggctggg ttgccatcgc cggcacacga cacgacggcc agggccatca
      541 gtgagagcgc aatccatctc gccacgatcg gtgacggtag atcccggccg accgatacac
      601 cgattcggcc gtaggctcgg gccggatgag atgggtccgg cgaatcctcg tggctttgct
      661 cctcgtcgcg gtggcgatcg gggtgttcgg ggtctttctg gttcgcaggt ccttccccca
      721 cgtcgagggc gagttgacgc tggccggcct cgacgatttc gtcgagatca tccgggatga
      781 ggacggtgtg ccccatgtgt acgccacgac cggtcacgac ctgttcttcg cccagggttt
      841 cgtccacgcc caggacaggt tctggcagat ggacttctgg cgccacatcg gggcggggcg
      901 actctccgag atgttcggtg acggccaggt ggaaaccgac atgttcttgc gctcgctcgg
      961 cttcaccgac ctggcggcgg aggaattgga atcgatgacc cccgagctcg tcgcgatcct
     1021 cgaggcctac tcggaaggag tcaatgcgta cctggccgac cggtcgcctt cccaggtcag
     1081 cctggagtac ggcatcctcc ccctgcaggc ttcgggctac gaaatcgagc cctggacggc
     1141 ggtcaacacc ctcacctggg ccaaggtcat gtcatgggac ctgagctgga acctgctgca
     1201 ggagatcgat cgagcgaccc tcagctcacg gctgccattg gagcggatcg agcagctgta
     1261 tccgggttac cccgaggcca acccggtggt ggtggcgtcg gaccagaccg cctcggtgtc
     1321 gagaacaggc cccaccatcc cccctgctgc tcgggaggct ctcaccgcag cggggcaacg
     1381 ggccgggctc gcttggacga tgaccggggg tggcttcgca ggcatcgggt cgaacaactg
     1441 ggtggtggga ggctcgcgca ccgaatccgg gctacccatc ctggccaatg acccccacct
     1501 ggcgatccag atgccttcca tctggtatca gaacgggctt cattgcgtgg agatcacaac
     1561 cgactgtccc ttccaggtgg ccgggttctc gttcgcggga tccccgggtg tgatcatcgg
     1621 ccacaacgac cgcatcgcct ggggagtgac caacgagtcg gtcgacaccc aggacctgtt
     1681 catcgagagg ctcaaccccg acgaccccgc ccaatacgag tacgaggggg agtgggtcga
     1741 catggaggtc agggcggaga ccatcgaggt cgccggagga gagaatgtca cctacgacgt
     1801 gatgtcgacc cggcacgggc cgatcatctc cgatctctac ctggacgagg ggcagttcga
     1861 cgactcctcg ctcgaccttc ccgacgagta cgcggtcagc ctctcctggc agaccctgga
     1921 gccatccacc ctggtcgagg cgatcgtcgg gctcaaccgg gccacgggct atgacgactt
     1981 caggaccgcg ctctccaagt gggatatcgc agcgcagaac gtggtgtatg ccgatgtcga
     2041 agggaacatc gcctatcagt cgaccgggga ggtgccgatc cgggccgccg gtgacggaag
     2101 atggccgatg ccggggtgga cgggtgagca cgagtggatc ggcgtggtgc cattcgagga
     2161 catgccgagc ttgctcaacc cctctccc
//
LOCUS       DATITW010000814         1826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_280051, whole genome shotgun sequence.
ACCESSION   DATITW010000814 DATITW010000000
VERSION     DATITW010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280051"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VLS86_11340"
     CDS             complement(<1..578)
                     /locus_tag="VLS86_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51133.1"
                     /translation="MGTDVLELPATLFQRLSAEGVDYVHWKSNEHLAAALRGQTDLDL
                     LVAVTHKGRFEKVLDDLGFVPLAPARARHIPGSESHLGFDRSTGTLIHLDVQYQLIVG
                     EQLLKNHRLPLDAWLLDDPGDLHGVSVPQPERELLLLYVRTMLKTTTRQLLRSRVKGG
                     SLVPDRILKEAVWLAERVTPEALRSAAETSGL"
     gene            complement(596..1387)
                     /locus_tag="VLS86_11345"
     CDS             complement(596..1387)
                     /locus_tag="VLS86_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSO51134.1"
                     /translation="MTGAFWRIDFIKATNYPLALVMKGLSAFIPIFTFVFVAQLVGSD
                     TADVGFDYYTFVVIGVVTMYILAATLNSFGNDMLELVTQGQLEMFLVEPVRWNLLPFA
                     MTPWAAFQASATAAVMVLWSVPLGAEYRLEAVPMALLIVVLGLAATLAIGILGASIKI
                     LSKRADPVLSLYTLAASVLSGVFFPVEFLPRWLQAMAWVIPHTYVIQALRRVLMPEGE
                     VLPGPTAWQAVAALSIFSLVFYPIALWVFGRILDYGRKIGALSGY"
     gene            complement(1423..>1826)
                     /locus_tag="VLS86_11350"
     CDS             complement(1423..>1826)
                     /locus_tag="VLS86_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51135.1"
                     /translation="KIATESNLAVLISSHRLDEVEMLNDQVLLLNKGHMVYNGPLEAM
                     MTSPLRAKVEIVFDEPGAAGIAAGTLQRVVGIEDVTVEENTVLLDSELPLSQIVKELD
                     GQVDHVSSIGHARPKLRDVLEQMLRPGQGDP"
BASE COUNT          336 a          579 c          631 g          280 t
ORIGIN      
        1 tcgagccccg aggtctccgc agccgagcgc agggcctcag gggtgacccg ctccgccagc
       61 cacaccgcct ctttgaggat gcggtcgggg accagcgagc ctcccttgac ccgggagcgg
      121 agcagctgac gtgtcgtcgt cttcagcatc gtccgcacgt agagaaggag aagctcccgc
      181 tccggctggg gcacgctgac cccgtggagg tccccagggt cgtcgagaag ccaggcgtcc
      241 agcggaagcc gatggttctt cagcagctgc tcgccgacga tcagctggta ctggacgtcg
      301 aggtggatca gggtgccggt cgaccggtcg aatccgagat ggctctccga ccccgggatg
      361 tgccgggccc gggccggggc gagggggacg aagccgagat cgtcgagcac cttctcgaat
      421 cggcccttgt gggtcaccgc caccaacagg tcgaggtcgg tctgacctct cagcgcggcg
      481 gcgaggtgct cgttgctctt ccaatggacg tagtcgacgc cctcggccga gagccgttgg
      541 aagagggtgg ccggcaactc gaggacgtcg gtccccatca gcgcacgccg gtcatctagt
      601 agccgctgag agcgccgatc ttgcgaccgt aatcgaggat gcggccgaag acccagagcg
      661 cgatcgggta gaagacgagg gagaagatgg agagggcggc caccgcctgc caggcagtcg
      721 gaccggggag gacctcgcct tcgggcatca ggacccgacg cagggcctgg atcacatagg
      781 tgtgagggat cacccaggcc atggcctgca gccatcgggg caggaactcg acggggaaga
      841 agaccccgga caacaccgat gcggccaggg tgtacaggga gagcaccggg tcggcccgct
      901 tggacaggat cttgatgctg gcgccgagga tgccgatcgc cagggtggcg gccagaccga
      961 gcaccacgat cagaagcgcc atcggcaccg cctccagccg gtattcggcc ccgaggggca
     1021 cggaccacag caccatgacc gcggcggtgg cggatgcctg gaaggcggcc cacggggtca
     1081 tggcaaaggg gagcaggttc cagcgaaccg gttccaccag gaacatctcc aattgcccct
     1141 gggtcaccaa ctcgagcatg tcgttcccga aggagttcag ggtggcggcc aggatgtaca
     1201 tggtcaccac cccgatcacc acgaaggtgt agtagtcgaa accgacgtcg gcggtgtcgg
     1261 acccgaccag ctgggcgacg aagacgaacg tgaagatcgg gatgaaggca ctcagccctt
     1321 tcatcaccag cgccagcggg tagttggtcg ccttgatgaa gtcgatccgc cagaaggccc
     1381 cggtcacctg gcgcccgcgt cggatacggc gcatgaagga tctcacgggt ctccctgccc
     1441 gggtcgcagc atctgttcca ggacatctcg cagcttgggc cgggcgtggc cgatcgagct
     1501 cacgtgatcg acctggccgt cgagctcctt gacgatctgg gacaggggca actcggagtc
     1561 gagcaggacc gtgttctcct ccaccgtgac gtcctcgatg ccgacgacac gctgcagggt
     1621 gcccgcggcg atccccgcgg cgcccggctc gtcgaagacg atctcgacct tggcccggag
     1681 tggcgaggtc atcatcgcct ccagcggccc gttgtagacc atgtggccct tgttgagaag
     1741 caggacctgg tcgttgagca tctccacctc gtcgagccga tgcgagctga tgagcaccgc
     1801 gaggttgctc tcggtggcga tcttct
//
LOCUS       DATITW010000815         1866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_400498, whole genome shotgun sequence.
ACCESSION   DATITW010000815 DATITW010000000
VERSION     DATITW010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400498"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VLS86_11355"
     CDS             <1..405
                     /locus_tag="VLS86_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSO51136.1"
                     /translation="GVADLDEALGVFFDHGVGMVLLTAGEHGASVITSDQVVNQRGFE
                     VDAVDATGCGDAFCAGIIEYLLRSGSLPTRRILDGLGPEDLAAMLARGQVVGASAATQ
                     PGCVEGVSKQTVDRIWSHQGELVLKSTAISRR"
     gene            409..1491
                     /gene="iolN"
                     /locus_tag="VLS86_11360"
     CDS             409..1491
                     /gene="iolN"
                     /locus_tag="VLS86_11360"
                     /EC_number="3.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-scyllo-inosose hydrolase"
                     /protein_id="HSO51137.1"
                     /translation="MEHPEPEFESNPVGELKRRIWEASDEEIDRILIEDYAIPSPGEM
                     ETPGCYIQMTHPADQERKLAKNDVVLIPLGSTELHGPHSVSAQDTLQVTRLVEGVRRY
                     TAEEGFEVNLALSPWIYGNHPKHHIGMIGTIPISSGVLERQLVDVMFGLWSQGYRKMV
                     FVNNHAQHWIIASAIDMFALRYPELPFFAVAVDWCCAMGEFFRTNDRGGPFEDDFIHA
                     DEAETSLALLLAPETVDMAQAVDTTPTGYLPDGHFNKSAAQRSRPNLWWSVRNNSPLE
                     FRATPVGVVGRATLASADKAKRAVAAALGYLTLLVGDILETFPPGVLPPIEEITLFRQ
                     EEIEGFLKKPGEPGYRNPYLLWRPFE"
     gene            complement(1508..>1866)
                     /locus_tag="VLS86_11365"
     CDS             complement(1508..>1866)
                     /locus_tag="VLS86_11365"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSO51138.1"
                     /translation="WLDRYVAAWRANEPGPIADLFSEDAVYRFQPYHEGEDVLRGRDQ
                     IVAAWLETPDDPATWEASYQAFAVDGDRAVATGISRYLPTGDTEERVYDNCFLMRFSP
                     DGRCTEFTEFYVRRPK"
BASE COUNT          327 a          595 c          610 g          334 t
ORIGIN      
        1 ggggtggctg atctcgacga ggccttgggg gtgttcttcg accacggggt gggcatggtc
       61 cttctcacgg ccggcgaaca tggcgcaagc gtcatcacct ccgaccaggt ggtcaatcag
      121 cggggcttcg aggtcgatgc cgtcgacgcg accgggtgtg gtgacgcgtt ctgtgctggg
      181 atcatcgaat atctgctccg gtccgggtca ctcccgaccc ggcgcatcct cgacgggctg
      241 ggccccgaag acctggctgc gatgctggcg cgaggtcaag tcgtcggagc ttcggctgcc
      301 acccagccgg gttgcgtcga gggggtgtcg aagcagaccg tcgaccgcat ctggtcacat
      361 caaggggaac tggtactcaa gtcgaccgcg atatcgagga ggtaagaagt ggaacacccg
      421 gagccggagt tcgaatccaa cccggtggga gagctgaagc gtcggatctg ggaggcatcc
      481 gacgaggaga tcgatcgcat cctcatcgag gattacgcca ttccttcccc aggcgagatg
      541 gagaccccgg gctgttacat ccagatgacc caccccgccg atcaggagcg gaaactcgcc
      601 aagaacgacg tcgtgctcat cccgctgggc tcgaccgagc tgcatggccc gcacagtgtc
      661 agcgcccagg acacgttgca ggtcaccagg ctggtcgagg gcgttcgccg ttacacggcg
      721 gaggaaggct tcgaggtcaa cctcgccttg tccccctgga tctacgggaa ccatcccaag
      781 caccacatcg ggatgatcgg caccatcccg atcagctccg gcgtgctcga gcgccagctg
      841 gtcgacgtga tgttcgggct gtggtcccag gggtaccgga agatggtatt cgtcaacaac
      901 catgcccagc attggatcat cgcctcggcc atcgacatgt tcgcgctgcg ctaccccgaa
      961 ttgccgttct tcgccgtggc cgtcgactgg tgctgtgcga tgggcgagtt cttccgcacc
     1021 aatgaccgcg gcggcccttt cgaggacgac ttcatccacg ccgatgaggc ggagacctcg
     1081 ctggcgctgc tgctcgcacc cgagaccgtg gacatggctc aggccgtcga caccacaccc
     1141 accggctatc tgcccgatgg gcacttcaac aagtcagcgg cgcaacgcag ccgtcccaac
     1201 ctgtggtgga gcgtgcgcaa caacagtccg ctggagtttc gtgccacccc ggtcggtgtg
     1261 gtgggacggg ccacgttggc ttccgccgac aaggcgaagc gagccgtcgc cgccgccctc
     1321 ggctatctga cgctcctggt cggggacatc ctcgagacat tcccaccggg ggtcctgcca
     1381 cccatcgagg agatcacctt gttccgccag gaagagatcg aaggcttcct caagaagccg
     1441 ggtgagcccg gttaccgcaa cccttatctg ctttggcgtc cattcgagta ggtgtgcggg
     1501 gtgagggtta cttgggccgc cggacgtaga actcggtgaa ctcggtgcat cggccatcgg
     1561 ggctgaaccg catcaggaag cagttgtcgt agacccgttc ctcggtgtcc ccggtgggga
     1621 ggtaacggct gatcccggtg gcgacggcac ggtcgccatc caccgcgaac gcctggtagc
     1681 tcgcctccca ggtggcgggg tcgtccggtg tctccaacca ggcggcgact atctggtccc
     1741 gaccccgaag gacgtcctca ccctcgtggt agggctggaa gcggtagaca gcgtcctcgc
     1801 tgaacaaatc ggcgatgggt cccggttcgt tcgcccgcca ggcggccacg tatcggtcga
     1861 gccagg
//
LOCUS       DATITW010000816         1626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_159982, whole genome shotgun sequence.
ACCESSION   DATITW010000816 DATITW010000000
VERSION     DATITW010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159982"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VLS86_11370"
     CDS             <1..278
                     /locus_tag="VLS86_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51139.1"
                     /translation="VYTVVAYAAIRTREPAELGRVTIAIWAGMLTLVGVVLLILIGTL
                     FGQVSLLGENAASRMILVPLANVAVAALLAPTFVRLVDRDRTALRYP"
     gene            275..>1626
                     /locus_tag="VLS86_11375"
     CDS             275..>1626
                     /locus_tag="VLS86_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HSO51140.1"
                     /translation="MKFALRLATLGVVFISMFSVIGLRLWFVQVAEGPEIAQAAEEST
                     WIGKSSYASRGDIFDRNGTLLATSRLVPAVMIDRTFVQPEQREALIQQLAALVGVDPL
                     ELDTLYEEAGINGRFQVSTISNEMALALNESLQDLPGVEVVKVPERVYLSGDTLAHVI
                     GHLGLPEAADIAANEDLDRNIRIGKLGVERVYDDILQGTSGYVEYRVRRGEIIEQKPP
                     VEPVPGSSVVLNLDLELQDLVEIALEEGVALSNSVKEDARANGDEVFSETQRAAAVVL
                     DVNTFDVLALASFPDFDPQLFVTGIDETTFNGLNETRAFNNLAVSGLYPPASTFKAIT
                     YTAKLEEDLPFPTDVEGVDGDLIHCDGTLVLSELEDGSQQLKHDWYDPRNLGWLDIHG
                     ALQTSCNIYFWNVALGTWLRFRNTAKENILQDWATRVGYGHETGIDLTSEAPGIVPTR
                     "
BASE COUNT          301 a          501 c          524 g          300 t
ORIGIN      
        1 tcgtgtacac cgtggtggcc tatgccgcga tccggaccag ggagccagcc gaattggggc
       61 gggtgacgat tgcgatctgg gctggtatgc tcacactcgt cggggtcgtg ctcctcatcc
      121 tcatcggcac actgtttggt caggtgagtc tcctcgggga aaacgcagca agccgtatga
      181 ttctggttcc gctggccaac gtcgcggtgg cggctttgct ggctccgacg tttgtgcggc
      241 tggtcgaccg tgatcgaacg gcgctgaggt atccatgaag ttcgctctga gactcgcgac
      301 cttgggtgtg gtgttcatct ccatgttctc ggtgatcggg ctgcggctct ggttcgtgca
      361 ggtggccgag ggcccggaga tcgcacaagc ggccgaggaa tcgacctgga tcgggaagtc
      421 gtcctatgcc tcccggggcg acatcttcga ccgcaacggg acactcctcg ccaccagtcg
      481 cctggtcccg gccgtgatga tcgatcgaac ctttgtccag cccgagcaac gcgaggccct
      541 catccagcag ttggcggcgc tggtaggcgt cgacccgctc gagttggaca ccctctacga
      601 ggaggcaggg atcaacgggc gcttccaggt gtcgaccatc tccaacgaga tggccctggc
      661 cctcaacgag tcactccagg acctccctgg ggtggaagtg gtgaaggtcc ccgagcgggt
      721 gtacctctcc ggggacaccc tggcccatgt catcggccat ctcgggctcc ccgaggcggc
      781 cgacatcgcg gcaaacgagg atctcgatcg caacatccgg atcggaaagc tgggcgtgga
      841 gcgggtctac gacgacatcc tccaggggac cagcggatat gtcgagtacc gggtgcgaag
      901 aggcgagatc atcgagcaga aaccaccggt cgagccggtg cccggaagct cggtggtcct
      961 caacctggat ctggagcttc aggatttggt cgagatcgct ctggaggaag gtgtggccct
     1021 ctccaactcc gtgaaggaag acgccagggc caatggggac gaggttttca gcgagaccca
     1081 acgggcagct gcagtcgtcc tcgatgtgaa caccttcgac gtgttggcgc tggcctcgtt
     1141 ccccgacttc gatccccaac tgttcgtgac cgggatcgac gagaccacct tcaacggtct
     1201 caacgagacc cgcgccttca acaatctggc ggtcagcggt ctctacccgc ctgcctcgac
     1261 cttcaaggcg atcacctaca ccgcgaagct ggaagaggac ctgccgttcc ccaccgatgt
     1321 ggagggggtc gacggtgatc tgatccactg cgacggcacg ctggtcctgt ccgagctcga
     1381 agacgggtcc cagcagctca agcacgattg gtacgacccg cgcaacctgg gatggctgga
     1441 catccacggc gccctgcaga cctcctgcaa catctacttc tggaacgtgg cgctggggac
     1501 ctggttgaga ttcaggaaca cggccaaaga gaacatcctc caggattggg cgacccgggt
     1561 cggctatgga cacgagaccg ggatcgatct gaccagcgag gcgcccggga tcgtgcctac
     1621 caggga
//
LOCUS       DATITW010000817         1900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_360384, whole genome shotgun sequence.
ACCESSION   DATITW010000817 DATITW010000000
VERSION     DATITW010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360384"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VLS86_11380"
     CDS             <1..1004
                     /locus_tag="VLS86_11380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSO51141.1"
                     /translation="VTKTQVDEACADSRAALEDYRAAQGAFQAAAEEYEVAVVDVDRV
                     ERKQEAVAGSVTSHSEELDLIQGKIEEQAVELYMRGGLSSPGIILSASSVDEYMTTSE
                     FLTAAATGGQESISELIAARSELDRFQGELAETHVELEAVETQKLDAKDAQEAAMEAE
                     QAAYAKLSGRCQELAARYDAEQAELKARAQQRASGSVQVGSFICPFTPGRTSFIDSWG
                     YPRSGGRRHKGTDMFAAWNEPMYAVTAGRVLLGSSGLGGKTIWLLGSNGIAFYYAHLS
                     DFNVSSGSSVSQGQTIGFNGDSGNARGGSPHLHFEIHPGGRGSAAVNPYPTVAAACR"
     gene            1015..1545
                     /locus_tag="VLS86_11385"
     CDS             1015..1545
                     /locus_tag="VLS86_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51142.1"
                     /translation="MTEQRRRIDMVLEPDYLDDLTEIDFDELRQRRETAEDVENQISY
                     YRRLLHGRMDLLNFELRRRSGDEKRTLLEALPEILAQGMIVGDEPTLRHLETMPPLPT
                     TTGRRLIDRIMDDGILTQLPELDQEELSEAIERLQEVEAQMSVQRRQLHTVIDALQEE
                     IITRYRSQQGEAQVTG"
     gene            1542..>1900
                     /locus_tag="VLS86_11390"
     CDS             1542..>1900
                     /locus_tag="VLS86_11390"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HSO51143.1"
                     /translation="MNLLAAKTRSGLVETIHEGAVAVVSGDGDLLAWSGDIDRPFFLR
                     SAAKPFQAAVSQEEGADLQPTELALAAASHDGEPVHVAIVEAMLASVGLTSSDLQCPP
                     SRPLSETAWRRNQAAGD"
BASE COUNT          340 a          638 c          623 g          299 t
ORIGIN      
        1 ctgtgacgaa aacccaggtc gacgaggcct gtgccgactc gcgggccgcg ctcgaggact
       61 accgggccgc ccagggcgca tttcaggcgg ccgcagagga gtacgaggtt gcggtcgtcg
      121 acgtcgaccg ggtggagcgc aagcaagagg cggtggccgg ctccgtcacc agtcactccg
      181 aggagctgga cctgatacag ggcaagatcg aggagcaggc ggtggagctg tacatgcggg
      241 gcgggctctc cagccccggg atcatcctct cggcctcctc ggtcgacgag tacatgacca
      301 cctccgaatt tctcaccgcc gcggccaccg gtggccagga gtcgatctcc gagttgatcg
      361 cggcccggag cgaactggac cgcttccagg gcgagctcgc ggagactcat gtggagttgg
      421 aggcggtcga gacgcaaaag ctcgacgcca aagacgccca ggaagctgcg atggaggccg
      481 agcaggctgc ctacgccaag ctgtcaggcc ggtgccagga gcttgccgcc cgctacgacg
      541 cggagcaagc cgagctcaag gccagagccc agcagcgagc cagcgggtcg gtccaggtcg
      601 gctctttcat ctgcccgttc accccgggtc gcacctcgtt catcgattcc tggggctacc
      661 ccaggtccgg gggtcggcgt cacaagggca ccgacatgtt cgccgcctgg aacgagccga
      721 tgtatgccgt caccgcgggt cgggtcctgc tcggcagcag cgggctgggt gggaagacca
      781 tctggctcct gggcagcaac gggatcgcct tctactacgc acatctctcc gatttcaacg
      841 tctcgtcggg ctccagtgtc agccagggtc agaccatcgg gttcaacggg gactccggca
      901 acgcccgtgg cgggtcgccc catcttcact tcgagattca cccgggaggc cgtggctcgg
      961 cggcggtcaa cccgtacccg actgtggccg cggcctgcag gtaaccttga tctcgtgacc
     1021 gagcaacgac gccgcatcga catggtcctc gagcccgact atctggacga cctgaccgag
     1081 atagatttcg acgagctgcg gcagcggagg gagaccgcag aagacgtcga gaaccagatc
     1141 tcctactacc ggcgtcttct ccatgggcgg atggacctgc tcaacttcga gcttcgacgt
     1201 cgcagcggtg acgagaagcg gactctgctc gaggccctgc ccgagatcct ggcccaaggc
     1261 atgatcgtcg gcgacgagcc cacgctgcgg cacctcgaga ccatgcctcc ccttccgacc
     1321 acgaccgggc gccgtctcat cgacaggatc atggacgacg gcatcctcac ccagcttccc
     1381 gagctcgacc aggaggagct gagcgaggcg atcgagcgac tgcaagaggt ggaggcccaa
     1441 atgtccgtgc agcgccgaca gctccacacc gtcatcgacg cccttcagga agagatcatc
     1501 acccgctacc ggagccagca gggcgaagcc caggtcactg gttgaacttg ctcgcagcaa
     1561 agacccgctc gggtcttgtc gagaccatcc acgagggtgc ggttgcggtg gtctcgggcg
     1621 atggtgacct gttggcctgg tccggtgaca tcgaccggcc cttcttcctc agatcggccg
     1681 ccaagccgtt tcaggcagcc gtgtcccagg aggagggcgc cgacctccag cccaccgagc
     1741 tcgccctggc cgcggcctcg cacgatggcg agccggtgca tgtcgcgatc gtcgaggcga
     1801 tgctcgcctc ggtagggctc acctcgtccg atctgcaatg cccccctagc cgtccgctgt
     1861 cggagaccgc ctggcggcga aatcaggccg ccggtgacga
//
LOCUS       DATITW010000818         1617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U15965
            k141_200068, whole genome shotgun sequence.
ACCESSION   DATITW010000818 DATITW010000000
VERSION     DATITW010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936675
            Sequence Read Archive: SRR9691037
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 10:09:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,280
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,247
            CDSs (with protein)               :: 2,247
            Genes (RNA)                       :: 14
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 9 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200068"
                     /isolate="SMAG_U15965"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.92 N 102.81 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VLS86_11395"
     CDS             complement(<1..910)
                     /locus_tag="VLS86_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSO51144.1"
                     /translation="MHPVVTVLYVVLVAFIAQTLFFPQSSDLTGPLGYTVAITAAGIV
                     SLRGASKLDGRERLAWSMFGGGLLIASLGLAVFAIMSSAGADLPAFGPLDTFFIVSYL
                     LVCLSVVTLPHKEGRWSTRVLGLVDGLVGAIAVAAAAWVWFLSGYLELLAAAPPLQRA
                     IAMAYPILDVAILISVLMLSVRRTRYRFDRRLLMLSIGVTFQAAADLAFAATGAGSMF
                     AETQPFYPLNITAGLFLLAAASIVHLRPAPREFADRPTRWVLLIAPYGAAALMTLGLV
                     WHAFRAEADHVLLLAVATLVVVGLTIA"
     gene            complement(1019..>1617)
                     /locus_tag="VLS86_11400"
     CDS             complement(1019..>1617)
                     /locus_tag="VLS86_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSO51145.1"
                     /translation="TEQLLGREGWGPISVANLHRAIEAAKDRPVARLLTALGIRHVGG
                     TVARLLVRHFGGLPALMAASEGDIAEVEGIGPVIAHGVATWAMDPDNQSRVAKLGEAG
                     VRLEDEIDPSASSDLLAGATFVVSGTIPGFSREEAQAAIEERGGKAASSVSGKTTALI
                     VGDSPGASKSRKAEELGVPILDENGFKKLLEAGLSFLG"
BASE COUNT          339 a          535 c          513 g          230 t
ORIGIN      
        1 gggcgatggt gagccccacc accaccaacg tcgccacggc aaggagaagg acgtggtccg
       61 cttcagcacg gaaggcgtgc cacacgaggc cgagagtcat gagtgcggct gcgccatacg
      121 gagcgatcag aaggacccac cgggttggtc gatcggcgaa ttcccggggt gccggccgca
      181 agtggacaat ggaggccgct gccagcagga acagcccggc tgtgatattc agcgggtaga
      241 agggttgtgt ctcggcgaac atgctcccgg ccccggttgc ggcgaacgcc agatcggccg
      301 ccgcctggaa ggtgacaccg atcgaaagca tgagcagacg ccgatcgaag cggtatctgg
      361 ttcggcgaac tgacagcatc aggacgctga tcaggatcgc cacatcgagg atcgggtagg
      421 ccatggcgat cgcccgctgc agcggcgggg cggcagcgag gagctcgagg tacccggaca
      481 gaaaccaaac ccaggccgcc gcggcgactg cgatcgctcc caccaaccca tcgacgaggc
      541 cgaggacccg ggtcgaccaa cgaccctcct tgtggggaag ggtgacgaca ctcaggcaaa
      601 ccagaaggta ggacactatg aagaaggtgt cgagtggccc gaaggccggg aggtcggccc
      661 cggctgagga catgatcgca aacacggcca gacccaacga ggcgatcaga agccctccac
      721 cgaacatcga ccaggccagt cgctcccgcc cgtccagctt gctggcgcct cgcagggaga
      781 cgatgcccgc agccgtgatc gccaccgtgt agccgagcgg gccggtcaga tcagaagact
      841 gcgggaaaaa cagcgtctgt gcaatgaagg cgaccaacac gacgtagaga acagtgacca
      901 ccgggtgcat gcggacataa cgccagacag ggcttggcgc actggctgtc aatgggactc
      961 ccacagtcac caatcggcag attgcgctcg aacctgtaaa cgaagtgctt cgaccaggtc
     1021 aaccgaggaa ggaaagaccc gcttcgagga gcttcttgaa accgttctcg tcgaggatcg
     1081 ggacccccaa ctcctcggcc tttctcgact tggaagcccc gggagagtcg ccgacgatca
     1141 gagctgtcgt cttgcccgag accgagcttg cggctttgcc cccccgctcc tcgatggcgg
     1201 cctgtgcctc ctcacggctg aacccgggaa tcgtccccga aaccacgaac gtggcaccag
     1261 ccaacagatc cgaggaagcg ctcgggtcga tctcgtcttc gagacggacg cctgcctccc
     1321 ccaatttggc cacccgggac tgattgtcgg gatccatcgc ccaggtcgcc actccatggg
     1381 cgatcaccgg gccgatcccc tcgacctctg cgatgtcgcc ctcgctggca gccatcagcg
     1441 ccggcagtcc tccgaaatgt cgaaccaaca gcctggccac cgtccccccg acgtgacgga
     1501 tcccgagtgc ggtcagcagc cgggccaccg gtcggtcctt ggccgcctcg atcgcccggt
     1561 gcaggttggc cactgaaatg gggccccaac cctcgcgacc gagcagctgt tcggtgt
//
